LOCUS       DARBDV010000001       128929 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000001 DARBDV010000000
VERSION     DARBDV010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 128929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 128929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..128929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.001"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(12..1535)
                     /locus_tag="SCM56_000001"
     CDS             complement(12..1535)
                     /locus_tag="SCM56_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEG1108836.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            1787..2968
                     /locus_tag="SCM56_000002"
     CDS             1787..2968
                     /locus_tag="SCM56_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEG1108837.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(3028..4656)
                     /gene="treC"
                     /locus_tag="SCM56_000003"
     CDS             complement(3028..4656)
                     /gene="treC"
                     /locus_tag="SCM56_000003"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEG1108838.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(5053..7764)
                     /locus_tag="SCM56_000004"
     CDS             complement(5053..7764)
                     /locus_tag="SCM56_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1108839.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7774..8475)
                     /locus_tag="SCM56_000005"
     CDS             complement(7774..8475)
                     /locus_tag="SCM56_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1108840.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8661..9260
                     /locus_tag="SCM56_000006"
     CDS             8661..9260
                     /locus_tag="SCM56_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEG1108841.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9341..10354
                     /locus_tag="SCM56_000007"
     CDS             9341..10354
                     /locus_tag="SCM56_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEG1108842.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10462..11379
                     /gene="rbsK"
                     /locus_tag="SCM56_000008"
     CDS             10462..11379
                     /gene="rbsK"
                     /locus_tag="SCM56_000008"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEG1108843.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11407..11802
                     /gene="rbsD"
                     /locus_tag="SCM56_000009"
     CDS             11407..11802
                     /gene="rbsD"
                     /locus_tag="SCM56_000009"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HEG1108844.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11802..13175
                     /locus_tag="SCM56_000010"
     CDS             11802..13175
                     /locus_tag="SCM56_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1108845.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            13532..15676
                     /locus_tag="SCM56_000011"
     CDS             13532..15676
                     /locus_tag="SCM56_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEG1108846.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(15728..16768)
                     /locus_tag="SCM56_000012"
     CDS             complement(15728..16768)
                     /locus_tag="SCM56_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEG1108847.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(17045..18847)
                     /locus_tag="SCM56_000013"
     CDS             complement(17045..18847)
                     /locus_tag="SCM56_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1108848.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(18861..19613)
                     /locus_tag="SCM56_000014"
     CDS             complement(18861..19613)
                     /locus_tag="SCM56_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1108849.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(19622..19984)
                     /locus_tag="SCM56_000015"
     CDS             complement(19622..19984)
                     /locus_tag="SCM56_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEG1108850.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(20160..21032)
                     /locus_tag="SCM56_000016"
     CDS             complement(20160..21032)
                     /locus_tag="SCM56_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEG1108851.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(21058..21570)
                     /locus_tag="SCM56_000017"
     CDS             complement(21058..21570)
                     /locus_tag="SCM56_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HEG1108852.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            complement(21708..22499)
                     /locus_tag="SCM56_000018"
     CDS             complement(21708..22499)
                     /locus_tag="SCM56_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HEG1108853.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(22554..23162)
                     /locus_tag="SCM56_000019"
     CDS             complement(22554..23162)
                     /locus_tag="SCM56_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEG1108854.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(23155..23472)
                     /locus_tag="SCM56_000020"
     CDS             complement(23155..23472)
                     /locus_tag="SCM56_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEG1108855.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(23628..24356)
                     /locus_tag="SCM56_000021"
     CDS             complement(23628..24356)
                     /locus_tag="SCM56_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HEG1108856.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            24515..25396
                     /locus_tag="SCM56_000022"
     CDS             24515..25396
                     /locus_tag="SCM56_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG1108857.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(25633..26676)
                     /locus_tag="SCM56_000023"
     CDS             complement(25633..26676)
                     /locus_tag="SCM56_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG1108858.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(26679..27347)
                     /locus_tag="SCM56_000024"
     CDS             complement(26679..27347)
                     /locus_tag="SCM56_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1108859.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            27489..29825
                     /locus_tag="SCM56_000025"
     CDS             27489..29825
                     /locus_tag="SCM56_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1108860.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(29940..30251)
                     /locus_tag="SCM56_000026"
     CDS             complement(29940..30251)
                     /locus_tag="SCM56_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108861.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            30432..31907
                     /locus_tag="SCM56_000027"
     CDS             30432..31907
                     /locus_tag="SCM56_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEG1108862.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            31954..32538
                     /locus_tag="SCM56_000028"
     CDS             31954..32538
                     /locus_tag="SCM56_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEG1108863.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            32540..32959
                     /locus_tag="SCM56_000029"
     CDS             32540..32959
                     /locus_tag="SCM56_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HEG1108864.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(33240..33524)
                     /locus_tag="SCM56_000030"
     CDS             complement(33240..33524)
                     /locus_tag="SCM56_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HEG1108865.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(33876..34586)
                     /locus_tag="SCM56_000031"
     CDS             complement(33876..34586)
                     /locus_tag="SCM56_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HEG1108866.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(34583..36055)
                     /locus_tag="SCM56_000032"
     CDS             complement(34583..36055)
                     /locus_tag="SCM56_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEG1108867.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            36193..36666
                     /locus_tag="SCM56_000033"
     CDS             36193..36666
                     /locus_tag="SCM56_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEG1108868.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(36819..37370)
                     /locus_tag="SCM56_000034"
     CDS             complement(36819..37370)
                     /locus_tag="SCM56_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEG1108869.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            37475..38005
                     /locus_tag="SCM56_000035"
     CDS             37475..38005
                     /locus_tag="SCM56_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HEG1108870.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(38488..38931)
                     /locus_tag="SCM56_000036"
     CDS             complement(38488..38931)
                     /locus_tag="SCM56_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1108871.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(38947..40107)
                     /locus_tag="SCM56_000037"
     CDS             complement(38947..40107)
                     /locus_tag="SCM56_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEG1108872.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            40614..42314
                     /locus_tag="SCM56_000038"
     CDS             40614..42314
                     /locus_tag="SCM56_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108873.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(42416..43249)
                     /locus_tag="SCM56_000039"
     CDS             complement(42416..43249)
                     /locus_tag="SCM56_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEG1108874.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(43553..45718)
                     /locus_tag="SCM56_000040"
     CDS             complement(43553..45718)
                     /locus_tag="SCM56_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1108875.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            45978..48272
                     /locus_tag="SCM56_000041"
     CDS             45978..48272
                     /locus_tag="SCM56_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEG1108876.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            48265..48930
                     /gene="pgmB"
                     /locus_tag="SCM56_000042"
     CDS             48265..48930
                     /gene="pgmB"
                     /locus_tag="SCM56_000042"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEG1108877.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            48923..49930
                     /locus_tag="SCM56_000043"
     CDS             48923..49930
                     /locus_tag="SCM56_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEG1108878.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            49952..50971
                     /locus_tag="SCM56_000044"
     CDS             49952..50971
                     /locus_tag="SCM56_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1108879.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(51252..52619)
                     /gene="nhaC"
                     /locus_tag="SCM56_000045"
     CDS             complement(51252..52619)
                     /gene="nhaC"
                     /locus_tag="SCM56_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEG1108880.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(52729..54150)
                     /locus_tag="SCM56_000046"
     CDS             complement(52729..54150)
                     /locus_tag="SCM56_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1108881.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(54393..56270)
                     /gene="tdc"
                     /locus_tag="SCM56_000047"
     CDS             complement(54393..56270)
                     /gene="tdc"
                     /locus_tag="SCM56_000047"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HEG1108882.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(56561..57817)
                     /locus_tag="SCM56_000048"
     CDS             complement(56561..57817)
                     /locus_tag="SCM56_000048"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEG1108883.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(57929..58134)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            58397..59698
                     /locus_tag="SCM56_000049"
     CDS             58397..59698
                     /locus_tag="SCM56_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEG1108884.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(59767..60366)
                     /locus_tag="SCM56_000050"
     CDS             complement(59767..60366)
                     /locus_tag="SCM56_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1108885.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            60620..61501
                     /locus_tag="SCM56_000051"
     CDS             60620..61501
                     /locus_tag="SCM56_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1108886.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            61514..63124
                     /locus_tag="SCM56_000052"
     CDS             61514..63124
                     /locus_tag="SCM56_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HEG1108887.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(63201..63860)
                     /locus_tag="SCM56_000053"
     CDS             complement(63201..63860)
                     /locus_tag="SCM56_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEG1108888.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(64143..64808)
                     /locus_tag="SCM56_000054"
     CDS             complement(64143..64808)
                     /locus_tag="SCM56_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HEG1108889.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(64955..65560)
                     /gene="dhaL"
                     /locus_tag="SCM56_000055"
     CDS             complement(64955..65560)
                     /gene="dhaL"
                     /locus_tag="SCM56_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HEG1108890.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(65588..66598)
                     /gene="dhaK"
                     /locus_tag="SCM56_000056"
     CDS             complement(65588..66598)
                     /gene="dhaK"
                     /locus_tag="SCM56_000056"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEG1108891.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(66573..67019)
                     /gene="dhaM"
                     /locus_tag="SCM56_000057"
     CDS             complement(66573..67019)
                     /gene="dhaM"
                     /locus_tag="SCM56_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEG1108892.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            67122..67442
                     /locus_tag="SCM56_000058"
     CDS             67122..67442
                     /locus_tag="SCM56_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEG1108893.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(67554..68423)
                     /locus_tag="SCM56_000059"
     CDS             complement(67554..68423)
                     /locus_tag="SCM56_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG1108894.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(68519..69822)
                     /locus_tag="SCM56_000060"
                     /pseudo
     CDS             complement(68519..69822)
                     /locus_tag="SCM56_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(69819..70328)
                     /locus_tag="SCM56_000061"
     CDS             complement(69819..70328)
                     /locus_tag="SCM56_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108895.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(70503..72638)
                     /locus_tag="SCM56_000062"
     CDS             complement(70503..72638)
                     /locus_tag="SCM56_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1108896.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(72721..74376)
                     /locus_tag="SCM56_000063"
     CDS             complement(72721..74376)
                     /locus_tag="SCM56_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEG1108897.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(74378..74692)
                     /locus_tag="SCM56_000064"
     CDS             complement(74378..74692)
                     /locus_tag="SCM56_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEG1108898.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            74862..75122
                     /locus_tag="SCM56_000065"
     CDS             74862..75122
                     /locus_tag="SCM56_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEG1108899.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            75136..75450
                     /locus_tag="SCM56_000066"
     CDS             75136..75450
                     /locus_tag="SCM56_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEG1108900.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(76180..76734)
                     /locus_tag="SCM56_000067"
     CDS             complement(76180..76734)
                     /locus_tag="SCM56_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEG1108901.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(76860..78788)
                     /locus_tag="SCM56_000068"
     CDS             complement(76860..78788)
                     /locus_tag="SCM56_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEG1108902.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(79094..79810)
                     /locus_tag="SCM56_000069"
     CDS             complement(79094..79810)
                     /locus_tag="SCM56_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEG1108903.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(79811..81634)
                     /gene="glpO"
                     /locus_tag="SCM56_000070"
     CDS             complement(79811..81634)
                     /gene="glpO"
                     /locus_tag="SCM56_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEG1108904.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(81648..83144)
                     /gene="glpK"
                     /locus_tag="SCM56_000071"
     CDS             complement(81648..83144)
                     /gene="glpK"
                     /locus_tag="SCM56_000071"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEG1108905.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(83280..84740)
                     /locus_tag="SCM56_000072"
     CDS             complement(83280..84740)
                     /locus_tag="SCM56_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1108906.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(84730..86025)
                     /locus_tag="SCM56_000073"
     CDS             complement(84730..86025)
                     /locus_tag="SCM56_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEG1108907.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(86357..87034)
                     /locus_tag="SCM56_000074"
     CDS             complement(86357..87034)
                     /locus_tag="SCM56_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEG1108908.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(87064..88155)
                     /locus_tag="SCM56_000075"
     CDS             complement(87064..88155)
                     /locus_tag="SCM56_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HEG1108909.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(88256..88690)
                     /locus_tag="SCM56_000076"
     CDS             complement(88256..88690)
                     /locus_tag="SCM56_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG1108910.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(89274..90731)
                     /gene="ascB"
                     /locus_tag="SCM56_000077"
     CDS             complement(89274..90731)
                     /gene="ascB"
                     /locus_tag="SCM56_000077"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEG1108911.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(90728..92623)
                     /locus_tag="SCM56_000078"
     CDS             complement(90728..92623)
                     /locus_tag="SCM56_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1108912.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(92681..94456)
                     /locus_tag="SCM56_000079"
     CDS             complement(92681..94456)
                     /locus_tag="SCM56_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEG1108913.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(94506..96002)
                     /locus_tag="SCM56_000080"
     CDS             complement(94506..96002)
                     /locus_tag="SCM56_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG1108914.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(96196..97818)
                     /locus_tag="SCM56_000081"
     CDS             complement(96196..97818)
                     /locus_tag="SCM56_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEG1108915.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(98267..99580)
                     /locus_tag="SCM56_000082"
     CDS             complement(98267..99580)
                     /locus_tag="SCM56_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEG1108916.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(99825..100049)
                     /locus_tag="SCM56_000083"
     CDS             complement(99825..100049)
                     /locus_tag="SCM56_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108917.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(100069..100491)
                     /locus_tag="SCM56_000084"
     CDS             complement(100069..100491)
                     /locus_tag="SCM56_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEG1108918.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(100635..101345)
                     /locus_tag="SCM56_000085"
     CDS             complement(100635..101345)
                     /locus_tag="SCM56_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108919.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(101363..101860)
                     /locus_tag="SCM56_000086"
     CDS             complement(101363..101860)
                     /locus_tag="SCM56_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1108920.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(101897..102349)
                     /locus_tag="SCM56_000087"
     CDS             complement(101897..102349)
                     /locus_tag="SCM56_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG1108921.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(102484..103089)
                     /locus_tag="SCM56_000088"
     CDS             complement(102484..103089)
                     /locus_tag="SCM56_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEG1108922.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(103539..104159)
                     /locus_tag="SCM56_000089"
     CDS             complement(103539..104159)
                     /locus_tag="SCM56_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1108923.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(104161..105030)
                     /locus_tag="SCM56_000090"
     CDS             complement(104161..105030)
                     /locus_tag="SCM56_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HEG1108924.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(105053..105460)
                     /locus_tag="SCM56_000091"
     CDS             complement(105053..105460)
                     /locus_tag="SCM56_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEG1108925.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(105479..106768)
                     /locus_tag="SCM56_000092"
     CDS             complement(105479..106768)
                     /locus_tag="SCM56_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1108926.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(106795..107097)
                     /locus_tag="SCM56_000093"
     CDS             complement(106795..107097)
                     /locus_tag="SCM56_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1108927.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(107122..109215)
                     /locus_tag="SCM56_000094"
     CDS             complement(107122..109215)
                     /locus_tag="SCM56_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEG1108928.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(109704..110468)
                     /locus_tag="SCM56_000095"
     CDS             complement(109704..110468)
                     /locus_tag="SCM56_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HEG1108929.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(110579..111448)
                     /gene="mdcB"
                     /locus_tag="SCM56_000096"
     CDS             complement(110579..111448)
                     /gene="mdcB"
                     /locus_tag="SCM56_000096"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HEG1108930.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(111473..112549)
                     /locus_tag="SCM56_000097"
     CDS             complement(111473..112549)
                     /locus_tag="SCM56_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEG1108931.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(112573..113166)
                     /locus_tag="SCM56_000098"
     CDS             complement(112573..113166)
                     /locus_tag="SCM56_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HEG1108932.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(113170..114846)
                     /gene="mdcD"
                     /locus_tag="SCM56_000099"
     CDS             complement(113170..114846)
                     /gene="mdcD"
                     /locus_tag="SCM56_000099"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HEG1108933.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(114836..115135)
                     /locus_tag="SCM56_000100"
     CDS             complement(114836..115135)
                     /locus_tag="SCM56_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HEG1108934.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(115237..116883)
                     /gene="mdcA"
                     /locus_tag="SCM56_000101"
     CDS             complement(115237..116883)
                     /gene="mdcA"
                     /locus_tag="SCM56_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HEG1108935.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(116890..117801)
                     /locus_tag="SCM56_000102"
     CDS             complement(116890..117801)
                     /locus_tag="SCM56_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEG1108936.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            117921..118778
                     /locus_tag="SCM56_000103"
     CDS             117921..118778
                     /locus_tag="SCM56_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG1108937.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(118965..119327)
                     /locus_tag="SCM56_000104"
     CDS             complement(118965..119327)
                     /locus_tag="SCM56_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEG1108938.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            119673..121112
                     /locus_tag="SCM56_000105"
     CDS             119673..121112
                     /locus_tag="SCM56_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1108939.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(121119..123671)
                     /locus_tag="SCM56_000106"
     CDS             complement(121119..123671)
                     /locus_tag="SCM56_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEG1108940.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(123785..125116)
                     /locus_tag="SCM56_000107"
     CDS             complement(123785..125116)
                     /locus_tag="SCM56_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1108941.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(125082..126560)
                     /locus_tag="SCM56_000108"
     CDS             complement(125082..126560)
                     /locus_tag="SCM56_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1108942.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(126865..128700)
                     /gene="tdc"
                     /locus_tag="SCM56_000109"
     CDS             complement(126865..128700)
                     /gene="tdc"
                     /locus_tag="SCM56_000109"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HEG1108943.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT        37729 a        26958 c        22545 g        41697 t
ORIGIN      
        1 ttttttgcct atcagtgcaa aagcatttcc tgtgcaattt cacgtccatc cattgtatct
       61 ggataaaatg ttggccaatt ctccatttct ttcagcaata cttcttgact ttcatttcct
      121 gcatataaat gaaaatgttc ggagtcagcc ggtgcaatcg tgtgatcact gaattgaata
      181 tatctaaaag cgccactttc cttatcttct gcttcgaata gataccttac gccacggttt
      241 cctgcctcgt aggtcaagat ttctttccct acataacgat aatttgaacg tttcgttgtt
      301 ccatccttaa acgtaaatgc catctctttc tcactgatct tgattgaagc aatatctgtt
      361 tgatagccct tcgtataata ttctttatat tcttgtgctg tcttatcctg attcaatttt
      421 tctttatatt caaaaacagg atcaaaagtc ccatcttcta catacgtatc tactgtttgc
      481 caagttcctg cccaatctga aagagagcga tcttgtacat cctcgtcttc aaaataaccg
      541 ttgtagacgc tcttttcttt tttgggggtc aaagtatctt ctagcgggtt ttctgtctct
      601 gtcgtttttt tcaacgcttc gaggtttgct tccatgatag aaagatagtt ttctccattt
      661 tctatttgtt cattcgtcaa tccttccaaa ggattcaata cttctaaaga tacacctgct
      721 tcgtttgcta acgttctggc aatactatct ttggcgtttt cttcaaaata aatatagttg
      781 attccatgtt ctttaacaaa gtgtttcagt tcagcaattc tcgccgcaga cggttcttca
      841 ctagaagaaa gaccagcaat cgatacttga ttcaatccat attctaacgc tagatagttg
      901 aatgcagcat gttgcgtgac aaaagatctt tgttttgctt gcttaaaggt ttcttgataa
      961 agctgatgga gtgcttgaag tttctcggta taggctgctg cattttttgt ccatacttcc
     1021 tgtttttctg ggtaagcttc aatcagctga tcacgtatgt ttgctacctg cttgattgct
     1081 aaacttggag ctagccatac atgtgggtct agctcatgtg tatggccttc ttccccgtgt
     1141 tcatggtcat gatcatgtcc ttcttcactt cccggaagta aaagcatacc ttctgtcgct
     1201 tttatcgtat ggacattgcc ttcttgtaat gttttttgga ttgcggggac ccatgtttcc
     1261 atgttttcat cattataaac aaagatatct gtatcttgga tctttgccat gtctttagca
     1321 gaaggttcat aatcgtggga atcagtccct gctggaatca ataactctac ttctccttca
     1381 tccccgacga ttgcttttgt aaaatcatac atgggataaa aggtcgtcat cacgctcaat
     1441 ttttctttac tgtcctcggc tttcccactt gccccgcaag cagttaacat acctgctaaa
     1501 accagcatgg ccaatatacc tatcaatcgt ttcatttatg acactcctct tctcatcttc
     1561 tttcatttga atgttccgat tcgcttttcg caaaccgtaa tagttacgtt ttagcaacag
     1621 gatttaattt atcatatggt taaaagaact gtcaagcgtt ttcttatttt aattgatgag
     1681 aaagtttata tgaatgattg acgatatttt tgcaatcaac ctctatcatt ttccttttat
     1741 acccgctttt gtctataatg aagttataga taaaggagga aaaagagtga aaaaagaaac
     1801 agatacaacg aatcgtttga tccgcttttt aggaggaaaa acctcttact atgttttagg
     1861 aatagttatc ttacttgcgc tgatcattta ttttttccat cagatctcgt ttatattcaa
     1921 gcctgtcgga atcattatcg ctactatctt gcccccatta ttgtttgcac tgatcttgta
     1981 ttatcttttg gaaccagtca tcaaacgttt gagcaagcac ctttcgagga acttgtcagt
     2041 cattatcgtt tatgtgatta ttttattttt attggggctt ggaagcatat ggcttattcc
     2101 ttttcttgaa gaacaggcaa agacattgat cagtagtttg ccagatttgt ttcaagattt
     2161 tcaagagagt ctccgacaat ttttggagaa aacacctttt gccgattcat ttaatcaatt
     2221 ttttgcttct attgacgacg tgacgtctga tatagctgga tttattggaa attattggga
     2281 aagtggcgca caacggatca gcaacatttt ttcaactgtt acagctattt ttattacttt
     2341 atttactggt ccaattgtcg cttttttcct tttgagagat cctcaaaaat tttatcagtc
     2401 agttttagcg attgtcccac cagcatttcg tacagatttt aaaaatttga caaagattgc
     2461 taatcaacaa ttgggttcat ttctaaaggg gcaaatcatt gcttcattta tccttggagc
     2521 agtctactgg gtttgtttct tactgatcgg attagaattt gctagtgttt tagctatttc
     2581 tgctgggttg ttatgtatca ttccatatat tgggccgttt atcgccttct ttccaggttt
     2641 gatcattgca ttccaagact ctacttttat ggctgtcaaa tttgtcattg tctggtttgc
     2701 cgtacaactt ctccacggtg acttggtgat tcctcgagta atgggtgatc gtttacagat
     2761 ccatccgatt acgatcttaa tcgttttact tgtcatgggt gatctaatgg gaattgtggg
     2821 cgttattttt ggtattccta tctatacgtt agtcaaatta ctcgttactt ttattttccg
     2881 taaattcaaa caacgctaca ataaatttta tggcgataaa ggagagtatg aaaactcttc
     2941 attttctgaa gaagattatt ttaaataaaa cacaaaacgt cgatcttacg agaatagctc
     3001 gtaggaacga cgttttgtgt ttttgtttta atttttcagc aaagcaattg cctggtatgg
     3061 ttttagctcc agactttgat gaagaacagt atcggaataa ttatcgataa gaatattggc
     3121 ttctgtgaat ccttctggga tggcgatcct tgtatcttta tcatagaaat ttgttaatac
     3181 aagcagctgc tgtccttcaa attggcgaag aaagccataa acttccggat gatttgcttc
     3241 ataagcatga taatctcctt gtgcaacgat tggatatgtt tttctcagcc gaatcaattt
     3301 cttgtaatag gacaaaatag aatcttcaga agatagttct ttttctacat taatctcttc
     3361 tagcttgcct ccaactttta accaaggttt ccctgtagta aatcctgctt gttctgtgtc
     3421 tgaccattgc atgggtgttc gagagttatc gcgagatttt gctttgatcc gacgaaacgc
     3481 ttctttttct gtgaatcctt gttcaaggag taacttataa gcattcaaac tttctacgtc
     3541 aatatagtcg gaaatcgatg aatagtctgg gtcgatcatg ccgatttctt ctcccatata
     3601 aatatagggc gtcccacggt tcaaatggat catggttgct agcatctctg ctccttgttt
     3661 tcgatactga tcgataacta caaatcgatt gagcgcacgt ggctgatcat gattattaaa
     3721 aaatagagca ttccagccgt taccctcact catattttct ccccatgtat gaaatagtcg
     3781 ctttaattct gcaaaatcaa atggaggagt agaccattta ttcccatttt cataatcgac
     3841 tttcaaatga tggaaattaa agaccattga taattcttct ctactaggtt ctgtatacaa
     3901 gatacaattt tcgatcgtcg tagagctcat ttcaccgact gtgatgatag tggaatccgt
     3961 cccaaacgtt gcttggttca attcttttag atacgtatgg gtgattggtt tgtccgtata
     4021 ttcaggtttg ccatcatttt ctttattatt tttcaattgt tcgtctttac cgataacatt
     4081 gataacatcg aaacggaatc cttttacccc tttttccatc caaaaacgaa caactttgaa
     4141 tagttcctct cttactgctt ggttccgcca gttcaagtca gcttgtgtcc gatcatataa
     4201 gtgcaaataa tatttccccg tatctccaaa tggcgaccaa gcacttccac caaattttga
     4261 tacccaatcc gttggctctt ctcttaaaat gaagaagtcc tgataatgct ggtcccctgc
     4321 tagtgctttt ttgaaccatt catgttctgt cgaaacgtga ttcagcacca tatcgagcat
     4381 gatttctatc cggtatttct ttgcttcggc tattagctgt tcgaattcct ccatcgtacc
     4441 aaagatagga tcgatggctg tgtaatcaga gatatcataa ccgttgtctt tttgagggct
     4501 tggataaata gggtttagcc aaatcatgtc gatgcctaga tcattcagat acgggagttt
     4561 ttctttgatt ccttttaaat ctccaattcc atcaccatta ctgtctttgt aagatttggg
     4621 atagatttgg taaatgactt tgtctttaaa tgtcatggat atttacctcc attcaactga
     4681 tttattacct accacagctt atatatacaa gttatcattt attattataa ctgaacagaa
     4741 agcgtttgta aaatttatct ttatacattc taagaggtct gttgcccaat aaaaaacaag
     4801 agatcagaca tgtatttgtc tatcctcttg tttaaaatag caaaatattc ttgtttcttc
     4861 tataaatggt gttcaaaagc ttttatctac ggcaatcagt cttctcaaat gtaaaacatg
     4921 aggagctaat tttatttgag tattcttttt gctcgatgca ggacggcttt tttacaacct
     4981 ctgattcatg gtgttcaatt agcagctgac cctcgttatt agctcaaaac gctgatttca
     5041 caacctctgt ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt
     5101 gaaatgggta acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac
     5161 ataagcccac catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa
     5221 gatgaagtca gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa
     5281 gatgatattt tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt
     5341 tattgtagat aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac
     5401 gataaaagcc agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc
     5461 cattgattct tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc
     5521 tgtggcaagc agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg
     5581 cagcaaaaat gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga
     5641 catataaata aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc
     5701 tttttgatct tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc
     5761 tactttttgc gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc
     5821 agttaaatgg ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact
     5881 ttgcgaagca tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc
     5941 atttactttt tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga
     6001 tgaatccaat gtaacgattg cttgatagtc aatgatcggt ccaaattgtt tggctgaaac
     6061 agaacttaga gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat
     6121 tccgataatc gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt
     6181 ttgattaaag ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc
     6241 tggcttcggt gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa
     6301 aacgatcatt gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg
     6361 cggccagtca taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa
     6421 aatgatcctt ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga
     6481 ggctagactt gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc
     6541 ctttaatgtc ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt
     6601 gatcaatgcg gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg
     6661 atcagacaga tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta
     6721 tgatggttta gttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga
     6781 ggccagttca cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg
     6841 ttgaggtaat gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc
     6901 tgactgttgc gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg
     6961 tgctttgcca tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt
     7021 cagctcttct aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt
     7081 ttgctccatt ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa
     7141 atcaagatat aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa
     7201 ataatcgacg ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa
     7261 tggagaacgg acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat
     7321 ctgataaata tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg
     7381 gtttggtttt tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt
     7441 gttataagaa aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt
     7501 tcctgtttct acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa
     7561 gccaccagta gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat
     7621 cgttttttgc atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat
     7681 gacaataagg atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc
     7741 tttaagatat gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga
     7801 aggatgatca tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc
     7861 agcgatgggc gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct
     7921 gctagcgttt tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt
     7981 cggttcatca cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct
     8041 ctgctgttcg cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac
     8101 ttgcctcaat acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac
     8161 attttctttt gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc
     8221 tgttcgtcgg taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat
     8281 aatgatctga ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt
     8341 tcctgctcca cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat
     8401 atcgtcattt gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac
     8461 ttcgacatac ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat
     8521 cgaatgcgtt ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg
     8581 aatcctttaa tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt
     8641 tatttgaaag aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa
     8701 aaactgcttt tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc
     8761 cacacactgc agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta
     8821 ccgctgcctt gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac
     8881 aaactgatat gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga
     8941 aggattcttt gaaagataca gataatatgt tgactatcaa aaaatacaat ccaagtgctt
     9001 tttttggtac ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa
     9061 gcggtgtagc gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt
     9121 accatatcgt gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt
     9181 tcataaaaaa gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag
     9241 ctatccaaca aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt
     9301 catgggaaaa taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac
     9361 aatcaaagaa gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa
     9421 tggaaatacg gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga
     9481 attaggctat cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat
     9541 tggtgtactc gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga
     9601 ggatatcttg tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca
     9661 aaaagaaatc gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc
     9721 gacttctgct gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt
     9781 cattgttttg gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt
     9841 tcgcggtggt tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct
     9901 tgtctatcct gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc
     9961 tcttgatgtt taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa
    10021 acaaggtggc taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc
    10081 tttaaatgat gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat
    10141 ccctgaagac tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc
    10201 gaaactgaca acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt
    10261 gttagatcgg atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt
    10321 agaaaaaaga ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc
    10381 gaactattaa aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg
    10441 agatagttgg aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac
    10501 actacccttc gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt
    10561 tttcacgctg gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca
    10621 cggacatctt ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta
    10681 ctgacaaaag aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga
    10741 caagcaatga ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca
    10801 aataatgctt tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac
    10861 tttattattg ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca
    10921 aaagacgcag gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact
    10981 gaactacttg aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca
    11041 ggaatcagag tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta
    11101 ggtattggaa cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa
    11161 gaacacggta tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat
    11221 acatttattg gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg
    11281 atcctttatg gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct
    11341 atcccttacc gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa
    11401 acaaacatga aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg
    11461 gggcactttg ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa
    11521 aaaatagatt tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt
    11581 ttagaagaac ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg
    11641 gaaatattgg aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat
    11701 gaagaaatga aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca
    11761 ccttacgcca atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc
    11821 ctgaacagat ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag
    11881 acaatattga ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc
    11941 cagtagccaa tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac
    12001 tcgatagata tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga
    12061 cgcgtcaaat cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg
    12121 gtttgatatc ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa
    12181 aaggtctggt tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc
    12241 atattttgtc tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt
    12301 ctgctgccaa aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga
    12361 ttgaaccgaa tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg
    12421 ggattcctgt agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt
    12481 tcttttattc ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag
    12541 tatttgtacc tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc
    12601 ctgcgggtgt ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta
    12661 acggaccgat cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta
    12721 tccaatgggc tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa
    12781 tcgttccgtt gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc
    12841 tgcgaagtaa acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac
    12901 ttgcgggagt gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg
    12961 ttgctgcttg cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag
    13021 cgatcgcttt tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt
    13081 tctggtactt agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta
    13141 gcgggtttga tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtatgag
    13201 atgtttatca catatttcag tctttttctt tttaggatca ttatttttat aaatttttgt
    13261 gatactttcc aaactttggt ataatttaca tatgcataac catttttatc cccacttgtt
    13321 tatccccaaa caaaggtggt ctatgcaata aaatgaacgt caaagaaaaa cgaatgaatg
    13381 ctcgttgctt atttacaaat tgttttattt agaatacaca gacagattta tgaaaagaaa
    13441 aagcttttcg acacgattat ctgttccaaa aaatgtctaa tgaatgctga ataagacaga
    13501 aaatcaatga aaggaaaagg actagtttat tatgaaaaat aaaaaaacga tacgtttgct
    13561 agctgctact tggctgattg ctccgtctgt tttgtcgacc aataccgtat ttgccgtaga
    13621 taatcaaata gctgatcaaa cagtcacaac agatacgact acatccagct cagctactag
    13681 tgaaacaacg gattctttta cagaagaccc aactggaaca actaactcaa caacaggatc
    13741 gaccgagcca actgaaattg atccggctga tccatcagaa aatgaaaagc cagccgagac
    13801 atattccctt acaattgctc cttctttgat tggtaagatc caactgactg cttcgacagc
    13861 ggattcccct gaacagcaac ctttggatat tacggaaagt ggaacggtag aaggtttatt
    13921 agcggaaacg aaggtaactt acactattac gccagctgaa ggtgaacaaa ttgcttcagt
    13981 cgctgttaat ggtgccaaag aagtaaatta tgatgcaaag acctttacag taggtacgaa
    14041 caatttatct attactgcag aatttaaagc tggaccggat acttcaacag agaaaccaac
    14101 tgatcctagt gaagaagtcc ctagtacacc agatccggaa acgccaaaca aaccagatgt
    14161 gggtaataac aacaataata atggttcaaa taattcaaac aataataaca ataattcgaa
    14221 cagcgggaat tcttcgaatc aaacaaaccc atctggatcg aataacaatg gaaatactca
    14281 gagagaacca aaccatccaa acaatggttc atcgaatggc ggaacgaaca atgcttcatc
    14341 tcgtccaaat acagcaaatc aaagcggtat agagaatcca tcttctgctt cttctgattt
    14401 tgttgtaaaa tcaccaattg atgctgtatt gcctacaaat acaacgaatg tccaacaagc
    14461 aatcgttcga gaagcattca agcatcttgg aaaaccatat gtatggggag caaaaggtcc
    14521 aaatactttt gactgttctg gtttgactta ttacgtatat atgaaagcaa ccggtcatta
    14581 tatcggtggc tggacaggcg aacagcaata tgcaggtacg cagattccag taagtcaggc
    14641 ccaacctggc gatctcgtct tctggggacc ttcttccggt gttactcacc atgtaggtat
    14701 ctatattggg aacggacaat ttatccatgc gcctcaacca ggagataaag ttcgagtaac
    14761 ttctatttca gatttcacgc ctgattttgc tgtccgagta aacttagcag gtttacctgc
    14821 tgcttcaggt tcgctgacag gcggctcatc tattttagat ggattagatg gctcgttcca
    14881 tttcagtcaa aaccaaacaa ctgatcagtt cttgaaaaag attgcagatg atgcacagga
    14941 aatcgggcaa aaagaaggaa tctacgcatc agtcatgatg gctcaagcta tccttgaaag
    15001 tggatctgga aacagcttat tatcaagcga accaaatcat aacctttttg gtatcaaagg
    15061 atcatataaa ggaagcagcg tgaccttcaa tacacttgaa caagatagtt ccggacaaag
    15121 ctaccaaatc cgcgcacaat tccgtaaata tccttcttac aaggaaagtt tggaagacta
    15181 tgccgacttg atcaaaaatg gactgactgg caatcctgat ttctacaaac caacttggaa
    15241 gtcggaaaca aaagactaca aagaagcaac caaatatttg gaaggacgtt atgctacaga
    15301 tcgccaatat tcacaaaaat tgaacgcgat catcgaagcg tacgacctga caaaatatga
    15361 tgaaccgaaa aaagaagcag caacaactga agaagcaaac gctgaaatcc tcaatatgtc
    15421 taagcgattc gatgtgccaa tcaaatggac aacagatggc gtgccaatca caggattaga
    15481 attttcaacg ataaaacgcg tcaacaacgc ttcttccatc ttccgtgtcg cttcaatctg
    15541 ggatttgtgg aacaatttta cggcaagaca gataccagaa acaactactc gtacagttaa
    15601 agctgcaact gttcctgtac taaccttgtt aagtttagac aaatcattct ggcttgatct
    15661 gcctttaaag aaatagaata aattaaaaag gggatgtgac actgtcacac tcccttttta
    15721 atttattcta tttctttata tttattttgt attccgttac aaactcgcgt acttggttag
    15781 gttccagagt gacatctcca aaactttcaa gtcgctccgc tcctggcagt tcttgagtct
    15841 caaatgtgat accgccatga tttactaatt tattcccgcg catttccggt ccatcttctc
    15901 caaaatttgc tgtaaaaatc acgattcctg aagcatctgt ttttacttgg atcgatactt
    15961 tttcatctgg gcttgtcaat tccgcagcat ttagtaaacc agattgtttt ttcaacaaaa
    16021 atggatgatc gatgccgccc actatcattg attggcgaaa atcactggca aagacctcac
    16081 ctaatttttt aggacgttgg aagtcaaatg ctgtgccact tactgaaact acttctcctg
    16141 taggcgttgt atctttattg agcgtagcaa ataatcggct attcaaccaa agtatatgtt
    16201 cgtcgattga ctgtgctgga tcaccggtta aattgaaata gacatgattt gtaggattga
    16261 atactgtttt gcggtcactg atcccttgga ccgtcactcg ccatgtatca tcgttcgtca
    16321 gcgtataccg tacttctaca tctaaagttc caggaaatcc gtttcccata tctgggctag
    16381 tctgatagaa aatgaccgat gcttcgtttt caccattgat gactgtataa tgccactgct
    16441 tggcttcaaa gctaggtgca ccaccatgta aacaatgacc agtgtccggg tctgttttta
    16501 cttcgaacgt ttcatcatct aatttgaatg tgccattcgt aatccgtcca gccatacgtc
    16561 cgattgaagc accgatataa gggtcttttt ctagatattc ttttgcttca tcaaaaccta
    16621 agatcaactc tcgtttttca ttatcgatta ttgtttgaaa atgaacaacc cgtgccccaa
    16681 gatcactgac agacaaagat gtcccatttt gattcgtgat cgtaatcaag tgatagtttt
    16741 cgccaaaatt ttgttcattg atttccatgt tccttgcacc ctcttttctt tcatttgcac
    16801 tgattccagt atacatggaa accgttttct taacagctta ttccgtgagt aacgagaaaa
    16861 aatttactat attctaataa aagaccgtga aaaaaaggtt gtgacaaaaa tattgtcaca
    16921 acctcttttt ccgaacaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca
    16981 aacgaaaaat atgaggagct atttttgctt ggctgttctt attacttgat gcaggacagc
    17041 tttttcacaa tctctttttc aatacgattc cagtatataa ccatgtcgtc agtacatagt
    17101 agatgaaata caatacgaag aagatcgtaa acggaaccca aatatctttg tatggatttt
    17161 gcatgaatag accaaacatt tgtaatccga ataagacatg gactacacct aagattcctg
    17221 gtgctaagaa taacacgcct acttctctac ggatcgattg tttaagcaac ccgcgtcgtg
    17281 ttcctatctt ttcaagcatt tcataacgag caatgtcact cttgcttcct gacaagatct
    17341 tgaacatcaa acagctagca agcatggtca agaaagctaa acctaggaag aatcccataa
    17401 attcaaaacc agagaataga ccattataca cttcatacat ttcatatttc tggttgctgt
    17461 actctacttt ttgcaaagat ggattgttca ttttattttg agcaacaagt gtttcgattg
    17521 gtttcaggtt tgcttggaag tcttttacct gaactagctg taaagtggat tgtggcaagt
    17581 tcagttgatc aaattctgat tgactgacta ccttgatttc tttttcttgc tgttccggca
    17641 attcatagct tcgaagttcc tcttgtacag tcagttcatt cattttttta gcaggaactt
    17701 tttctttcgt gatcttcggc ggtgttccat cttcatgttt tatcatgatc aatggttgag
    17761 ccgcaaattc acttgcatta tagtaaatcg ttcccgcatc ttctttttga gtataagaag
    17821 cagaaagtgt tggtgaaagt tctttgattt tttgctgatc ttctgcccga ggattattca
    17881 tcactagatc atatccagaa ttgttttctg tcaagtctgc aatttcattt ttaaaaccta
    17941 atcctaccgt caaagcgccc agtgccaagg caaacagcat agcgaccatt gagagcattt
    18001 gtgtatattc acgaatacga aagtttaact gtgacaatgt gaaattgttc aattttttca
    18061 atgaaatctg ttctgatttc tttagtaatg ataaaaagaa aatgatcaca gaatggaaaa
    18121 tgaaataagt tcctaatatg atcgtaacta aggctacccc tataccaaag acttgatagg
    18181 tccccacgtg tttcatcata tagtaaccgc agcctagaaa agcaattccc agtactactt
    18241 caaggaaaaa caagaattta tgctgcttga ttcttgttgg tgtctgatcc gcacgaatca
    18301 atgctaaaat cggttttttg acaatcgaaa aagcattgac aacagcagct aataaaaaca
    18361 aaacagcaaa aaacaaaaca gtgatcaaca acccgtgaat actaaaaggt gtaaaatgcg
    18421 aaatattgag atctaactga tccactaata atcggtggac gatacttgtc aatcctacac
    18481 cgacagctga acccactaaa gtagctgcga tccctactaa gaatgtttca gaaaaaatca
    18541 tctgtgcgat ttttctgcct tttgctccca gcatcataaa cattgcatag tctttttgcc
    18601 gcatcgtcat caaaaaggaa tttgcatata aaatataaac gaatgtgatg atccctagta
    18661 aaacagaacc gaatcggaaa atgataactg tagccgcaac gatcgtatta cttttcaaaa
    18721 aagcttcgtt gcttgccatt gattcaaaca tgtaaaaaat agcagatgcc ataactaatc
    18781 ctgagaataa caccaagtag tctcttaaac ggctcttgat cccagtaagt gataatttcc
    18841 aaagcatagt caatctcccc ctactgttcc aagtttccaa gaatagatag tatttctcga
    18901 tagaaggaat cacggctttg atctccacgt ttcacttctt gatggatacc gccatccttg
    18961 atgaataaaa tacgttggca ataacttgca gaaaacggat catgcgtcac aagaagaatc
    19021 gatacatgat cttttgtatt caattcgtcc atcgtatcta aaagatcccg ggcacttttg
    19081 gaatccagcg cacctgtagg ctcatcccct aatagaatcg ttggttgtgt gatcaatgca
    19141 cgtgcggcgg cgacccgttg tttttgtcca ccggaaatct cagaaggata gctttccaaa
    19201 atatgagaga tggataaacg ttctgcaatt ttttggacac gttgcttgat ttcttttggt
    19261 ctgacgcctt gtaaagaaag aggaacagcg atattttctt gtgctgtcaa gctttctaat
    19321 aaattgaaat cttggaaaat aaagccaatc tcattcgcac gaaaatctgc tagttgattt
    19381 ccttttaatg tggtcacatc tttttgattg attcggatat gcccatctgt tggtttatct
    19441 aatgtggata aaatattcaa aagtgtcgac ttaccagaac cagaagcgcc catgatccca
    19501 ataaattcgc ctttttctac atcgaagctg atgccgctta atgcttgcgt ctttttctca
    19561 ttgttttttc cgtatacttt tgttacattc tgtacttcga ctattttgtt catttctttc
    19621 cctacccttt aattagtttt tccagtgctg ttttcggcac gtcttttttt tcgacttctt
    19681 catacgaagt gacaccttta tttttgttcc aggtgatttt taaatatgct cctttacgaa
    19741 gcggtcgagt caattgtccc ttgaagtcca gttctttttc cttaccctct ttcgtatagc
    19801 caggcaggat atatctatag ttgactgttt cttgtccaga gtctgatttt gaaactgttt
    19861 ttgtaccatc tgtcgtaatt tgtacatagt attcttctcc acccataact acgtaatctg
    19921 cgattttcac ccctgcaagt accacaacga tgaaaacaag tagaccgatc agaagttttt
    19981 tcactttttc cccttctctc ttactttata tactgattat agaaaaaagc gaacaattct
    20041 gccttcgaat caactatcat tcttctttca attttgtcat tttctttaaa acaactaaaa
    20101 aaagagttca agacaataat ccgtcctaaa ctccttcttc ctatcttaca aaaagtatgt
    20161 cacttacggt ctttcatgtc gcctgtattg ataacaggtt gcgttccttt gtctgtaatg
    20221 attgagacaa gtaaattatt gatcaattgg actttccgat catcggtgaa attgatttct
    20281 tgtccatctt cgatttgctc taaggccatt tgagtgattg tgacagctcc ttcaacaatg
    20341 gtttgtcgag cagacaaaat ggcttttgct tgttgacgtt ggagcatggc actagcgatt
    20401 tctgttgcat aggctaaatg gttcaagcgt gtttcgatta cttcgacacc tgctactgct
    20461 aaacgctctt gcagctcttt ttgcaactcg tcagacactg ccgtcgtgtt tcctcgaagc
    20521 gtcaaatcat tctcattgaa tgtatcatac gggtattggc tagcgatatg ccgaatggct
    20581 gtctcacttt ggatttctac aaaatcttga taatacgcta catcgaataa tgcctttgct
    20641 gtatcaacga ctttaaagac tactactgct gagatttcga tggggtttcc atccaaatca
    20701 ttgactttta aaacagaact attgaaattc cgaactttca acgaaacagt cattttttgt
    20761 gccaaaggta tcgtaaggaa aaagccattt tctcgaatcg tacctaaata ttgtccaaaa
    20821 aacaaaatta cttttgcttg attaggactg actacagttg ccgaacttaa caacaataac
    20881 gtgatcaacc aaagaaaaat acttaaaaaa agtgcccaaa ctttcgcttg ccacatacct
    20941 aaatagaaga aaaacaatcc aactaaagct aatacgacca atccgacaat ccctgcataa
    21001 ccattgatat aaaatgctct tttttctctc atgctacttc ctgcgctcct ttatccttca
    21061 ataaatatct aatgttactt ttttaatttt ttttcgaata cgcgccatta caccttctag
    21121 ttctaactct ttgtcttcag tgatgtacca accccttgcc tgtgtccctc cggctgcctg
    21181 cggtaaataa tccaattttg ttcgaaccca gtgcttttct tctgtaaaat agtcttctac
    21241 caagacctct agatccatcc ctgctgaaag aggcaaatct tcccgaacag atttaaagat
    21301 aaatcgttcc ttcttcccat catacttcaa tattcctgta tcgataatcg gtaaagaata
    21361 ataatccagc tctttcttca ccgtcaataa atgtttgaaa acttcatggt acacttgctc
    21421 taattggcga ccttctgaat tgttcgggta attaaccatc actttttctt tgtctggatc
    21481 atagaaggtt tggcttagtt gagtgatata agtatgtaat ctttccattt ctgtttttaa
    21541 cttttctctt tgaccttctt ctaccttcat atactctttc accctgtttt ctttttagat
    21601 attttctttc ttattttact atagttatgt gcgttaagcg atgaaaaaca gcaaagattg
    21661 gaaggatctt tgctgttttt tcttaaaata tccagttgag tacaggatca atctgtttca
    21721 ttgtcctctt gatccgtcgt aatattgatc gatgtgtctt tagcagtgat ctttagcttt
    21781 ttctgtcctt gttctaacaa agtatttgtt attggctggt agtctttttc aatataagat
    21841 tcaccagacg tttggatagt atagccactg ttttccatct catgcaaaga taaattgcca
    21901 ccgtttagga tgacttttcc ttctttgctg atcaaactgt cttctagatg gaagttgctg
    21961 ttttcacctg tgacgatcaa gttatccaca ttggcctcat ctaatcgaag tgagcctaat
    22021 tcatctttcg cagttattgt tcctcctgta aattcattga acgataccca gccatcatgt
    22081 gtcttagtat tcactttttt gattgatact tcgttgatat caattgcact attattccct
    22141 tcgattgtca attcagataa ttcatgatct gcagggacaa caatagtgat ttgtgcactt
    22201 gtgtcttcta aactaatgat tgcacctgtt gattttttga ctgcattcac agtcaacacg
    22261 ccatttttct cctttatttc tggcttttct gtcttatcat aagtcgtttt tatctcaaaa
    22321 tccaatccgc gtatgaccga aactgtttga ttttctgcat ggatgacgat tttgtttgca
    22381 tccttgattt tttttgtctc agtaaccata tcgatcaacc gaggaccatt atcccaagtc
    22441 agactctttt gagcgcctaa tgtataagtt gcacctgcta ggaggccacc agtaagcatc
    22501 agaccaatac cggtaatcaa aaaatgtttc aatctcaatt tatgtgtcct ccttcaaacg
    22561 cccgctgaat gcagatctct ttttgtaata aaacgtcgtc ccatccattt aacaaagttc
    22621 atcatgacat aaaaaagccc ttttgttcct ttcagcaaca gtggcgaaat aaatactgcg
    22681 cttcctgtgg cgaacagacc gacaccgata aagaataatc cactcagaat cgattgtcca
    22741 aacacactga ttcctgagat gatcatgaat aggccaacac ctaagatact gatacataaa
    22801 acatagatac taaaaatgag cgctagtaaa gcaaccaata gtcccccaat aaccaatata
    22861 aaccccagtg ctagtggaat gaggacagga gaagcaaata aaccgataat gattaaccaa
    22921 atcaggcgaa tttgtctttt tggatttttc actaactcgt tttctgcttg ttcttcttct
    22981 gaaaagtaac tgatttttaa ttttaaggca aactgctttg gtgtaccata ttcatcaatg
    23041 atctgatcta atgtttttcc agaatctaaa aaatattcct cataatactg gatcaaatca
    23101 aaacgatcgt cttcaggaat ttcttgaaga tgatccacaa cttcattcaa atattcattc
    23161 atttgtgctc tcctccaata accggtctaa agaatctcga aaaacaatcc attcatcaat
    23221 aatttcttct aaatgacgac ttcccattgg tgtcaacgag taatatcgac ggtttctgcc
    23281 ttcaaaaggt tcatcatagg tctcaagata tccttctttt ttcaatcttc tcaaaaccgg
    23341 atatagtgta gattcactga cagagagact ttcttgtacg atttttgtta aggcataccc
    23401 atacatatct tccttgctta atgctgctag cacagtacca tcaagaagaa cagttggaat
    23461 ctggatgtgc attacgtcac cttctattct caagtgtaat attttatttc aatctatatt
    23521 atacaacata taatatagtt tcaaaatcag tttgtcaaat acaaaaaaac tgactaaaag
    23581 atttgatcct ttttagtcag caaaaaatgg cttctctttg atttttatta taacagtggc
    23641 ataatccaat ttgtccacat ccagctcagt ggcatgataa cgatcattgc aggtatcatg
    23701 tcagctactg ggaacatttt cacgttgatc attctaaaac cggtagcgac catcaagaat
    23761 ccgccgcaag ctttaaaatc taagatcatg tctggtgtag tcaatggata aataaatttt
    23821 gccaataaga acaaaatata aaaaataatg aattggggaa ttgctatcac agaaacgaca
    23881 taccctaaat tacacgcaaa aatagctgct gtgaaaaaat caaggacaga tttagaaatc
    23941 aaaatcgtac tgtctcctgt catcccagaa tctaaacttc catagatccc cgtaccactt
    24001 gcacaaaata gaacaatgac cgtcactaat gtgctgataa attcatcgtg ggtcatacca
    24061 agcttttcac tagggaagat tttagaaata ggtacttgca tcagcgcagc ccccttattt
    24121 atcccattac ctaaatgaac aatcagacca atcgctgttc ctatcactaa agcaaaaata
    24181 acagccggca tatttttcat cggcgcaatg gaatagattc ccatccccat cgaacacaca
    24241 ccaaaaacca tcgtcagctg tgctttaaaa tgttcactta atttatttcc tactaatccg
    24301 ccaattagtc cgccaagcaa aacggaacct acattgatca aaactccagt tggcatcaaa
    24361 aaactcctct ctttttctac gaagcgaaaa aaatcagaaa aatttctgta attattgctt
    24421 tcgctgcaag aaagattgta acagacagag aagattacac caattcattt tttgtgttaa
    24481 agtattcttt attggaataa gtaggaggaa tatgatggat atccataaat tcgaagtttt
    24541 tcttgattta gccgaaacaa tgaatttcac caaaactgct gatcgacaat tcaccaccca
    24601 aggaaatatt tctaaacaga ttctttcgtt agaaacagaa ttagacgtca aattattcga
    24661 acgcgcccat agaaaaatcg aattgaccga agctggaagc ttattgctcc catatgtaaa
    24721 aaatgtcgta gaacaatacc atgctatgca agaagtgctt gaaacctata cgaaagacaa
    24781 caatctttca ttaaatatct taacgatacc gacaatgatc aattataaag gtttttccaa
    24841 aatcaccgag tttttgaaac gccatcctga atttacagtc cagttgaaag aagtggaaag
    24901 tgtcgaatta acttacaatg acgccaatga acatagtgac acgatccact ttgctagaaa
    24961 tttccaggcc ccaccaagtt cgattgagtg gttgcctact gaaacagacg attttgtagc
    25021 agttctacct aaaaatcatc ctttagctag taaaaagaaa ttggatctaa gcgaactgaa
    25081 aaaggagaac tttttgctcc ttggccccac tacgaacttg tatcaacctg tactagatct
    25141 gtgtcgcgaa gctggatttg aaccaaaaat ctcttataaa ggagcaagaa tcgacttgat
    25201 catcaatatg attgcaaatg acatggggat tgctattgtt atggaaaaaa cagtcaagaa
    25261 tctattaaaa gaaaatactg tgattctccc tatttctcca acaacagaga gttatcttgc
    25321 atttactcgt aaagtcggtg agcattcttt ggcttcagat acattttggg agtatttaaa
    25381 tcaagaagaa aactaagccc tacattttct gtccatctat ccacccaaaa aaagagagcg
    25441 acgcatgact ttcgctctct ttcttgcatc atgtccttgt ttcaatgaca cctctaacat
    25501 ttctcgtagt tcaataagaa agcagcgatt tttatgccaa aagaaccgta agaacatttc
    25561 cctaatcaca aaaaaacgac taaaaacccc taaaaacctt caatagaatt tttaggggtt
    25621 ttattctttt tcctattgtt tacttaaccg tatcattgct gtcgttccta ctccttcgat
    25681 actgctataa tcaatacgtc catcatgcag ttctagaatt tttttaacca gtgaaagtcc
    25741 tagcccattc ccagaaattt gacgacttgt atctccttga taaaattttt caaacattcg
    25801 atctctggtc tcttcattca ttcctacacc tgaatcggtc acttccagtc caatctctgt
    25861 ggctgtctca aatagtttga tatgaatctg tccattgatt tgattgtact ttatggcgtt
    25921 atccatgata ttcagccaca cttggtacaa aaactcttca ttgcctgtgt aatttactgc
    25981 agcaagttcg atatccaatt ctagctgttc tttctcccat ttaggctgca aaaaaacgat
    26041 tacttctctg atttgttcat ctaatcgata ctctttcttt tccagttgga tacgctggtt
    26101 ttccagtttg ttcaatttta agatgttctc tgttaattgt gaaagctgcg tgatactttc
    26161 gataatccga tataaaaata tttggcgttc ttcgtcagag agatttgggg cttgtaacaa
    26221 ttgaacatat ccttggattg ttgctagtgg tgttttgaat tcatgggata cactggacac
    26281 aaagtcattc ctcagtgtct cgatactatt gagttcttgg accataacgt tgaaatcttt
    26341 atacagttgc tgtacttctg ctactttctg ctgttcatcc attcgaatcg aaaaatctcc
    26401 cgttgctacc agactcatat ttgtccctag cgcactgatt ggatgcagga tacgccgccc
    26461 aacaaaaatc gatattcctg tacccacgac catactgaac cccgcaaaga cgaataacgg
    26521 gtataacgtt ggttttccag ccgattcagc aggcgagata gaatgtcgag acaataagaa
    26581 aataaaaccg agaaaacaca gcagtgtcac taagatcgaa atgaagacaa tactagcaaa
    26641 atagatccat aattgtgaaa atagcttttt tttcatcttt aaataaccgc cttatagcct
    26701 agtccccgta ctgtgatgat ttgaaaatct tccgtctttt caaaacgggt ccttaaacgt
    26761 ttgatatgta catcgatcgt tcgttcttct gtattcgtat ctaatcccca aatatcatcc
    26821 atcaactgtt ggcgagtgaa gatcttgttg ggataagaca gtaatttata cagaagcaga
    26881 aattcttttt gaggcagaat caacgaatgc tgatcttgta acactgtata agcatctagc
    26941 tctaattttg tttgtccgat tatcaagcta ctgctgtaat tgatctttga acgtcgaagc
    27001 aatgcttcca cacgtaggat catctcattg acatcgatgg gttttgtcat ataatcatct
    27061 gctcctaatt tgaacccgcg tttcttatcc tcaaaatcgg cctttgccgt aatcattaga
    27121 acaggtgtgt caaatctggt gctacgtaat gagtctagca agtcatagcc atccattttc
    27181 ggcatcatga tatctgtaat gattaattct atacgtgtcg cttctagttt tttcagcgca
    27241 tcctctccat tcaatgcggt gatgactgaa aagcccgctc gttccaacac agaacggtaa
    27301 agcaactgta attgcggatc atcttctact actaaaatac gattcattga tctccctctg
    27361 ttttttgaaa aatttctaga ttttttcttg tagttcatct tcagttaaca tttatttcgt
    27421 atactacgat cattgttctg atatacttat tagtataccg aatatttcaa tacgggagaa
    27481 gggtgattat gcttcaatta aaagatataa aaaaatatta taaagtcggc gaaaccacca
    27541 ccaaagcatt ggatggtgtg tctgtcgctt tcagacaaaa agaattcgta gcgattttag
    27601 gtcctagtgg ttctggtaaa acgactatgc tgaatgtgat tggtggacta gataattatg
    27661 attccggtga tatggtaatc aacggaaagt ctactaaaga ctttaaggac agtgactggg
    27721 atgcctaccg aaacaactct atcggtttca tttttcaaag ttacaacttg attggtcatc
    27781 taggcattat tgaaaacgtt gaacttggga tgacattaag tggtgtttct aaagacgaaa
    27841 aacgaaaacg ggcagaagat gcattgcacc gagtcggctt gacagatcat atgcataaaa
    27901 agccaaatca gttatctggt ggacaaatgc agcgagtggc catcgctcga gcactagcca
    27961 atgacccaga tatccttttg tgcgatgaac caaccggagc gttagataca gaaacaagta
    28021 tccagatcat ggaattgatc caagagttat cgaaagaaaa attagtgatc atggtcacac
    28081 ataatcctga actggcccat caatacgcag atcggatcat tgaattttct gatggtaaga
    28141 tcttaacaga ttcccatcct cacatcgaac gaccaaaaga tgatcaattt aacttacgca
    28201 gaacaaagat gagtttttgg acagcgttaa agctttcctt caacaatatc cgaacaaaaa
    28261 aaggacgtac tttcttgacc tcctttgctt ccagtattgg gatcatcgga atcgcgatcg
    28321 ttctgtccct ttcttctgga ttccaaaaac aaatcgataa cacgcaagct gaaacgatgg
    28381 cgaaatttcc gattactatt tctaaggtaa cgactaacca gactcgtgac gatgccggat
    28441 tgggtgcaag caaagcggat tatccagaca gcaaaacgat tactgcaaaa gtcagcgaag
    28501 aagataaagc acagcacacg aataagattg atcaaacata cgtcgattac gtaacagaca
    28561 tcgatcctaa cttaagtaat aatatcggat ttacccgtac tacaggaatc aatcttttac
    28621 gtgatgtaaa tggcaaagta cagcctgtca gcttctcgaa tcaaaatcct gatacggaat
    28681 ctctttcttt atctagtacg atgtcagcaa tgacaggtgt gggagtgtca agtttcccta
    28741 cacagcttga cactagcaaa gaaaactttt taaaagacaa ttattcttta cttgccggaa
    28801 gttatccagc ctcagcaaca gatgtcgtgc tgatcgtaga cggcaataat aatactaata
    28861 tcaacgcatt gaagaatctt ggatttgatg tgaaagaaga cgaaaaactt gattttgatg
    28921 atatcgttgg tacaacattc aaattagtaa acaacaatac ttactataca aaacttccaa
    28981 ctgggaattt tatccctaat acagattatg atgcgatgta tcagaatgct tctgatgaat
    29041 tgaagatctc ggggatccta cgcgtgaaat cttcttctac aatgaatctc ttatctccag
    29101 gaatcgctta tagcgatcaa ttaacgactc aaatcgtcaa cgaaaacaaa gaatcagaaa
    29161 tcgtcaaagc tcaaagagac agtggtgtaa atgtcttaac tactgaaaaa gttgacgaaa
    29221 atgccaaaca aacattgctt agttatttag gtggagacag cttgccttca agtatcatga
    29281 tttatccgaa caattttgaa gacaaagaaa agatcttgga ttatctagat gattacaata
    29341 aaggcaaatc agatgaagat aaaatcattt atactgattt agctggaacg atgactgaac
    29401 tgactggcgg attgatggat gctatcactt atgtccttat tgcttttgca gggatctctt
    29461 tagtaacaag tatgatcatg atcagtatca ttacttatac atctgtgatt gaacggacaa
    29521 aagaaatcgg tgtactaaaa gctttaggtg cacgtaaaaa agacatcact cgcgtatttg
    29581 acgcagaaac gtgtatttta ggtatttctt ctgggatatt aggtgtgttt attgcatggc
    29641 tagctacgtt ccctatcaat agcatcttat acaacatgac ggatctaaaa aatgttgctc
    29701 aactaaatcc tgtccatgcg attattctag tgatcgtctc tactatattg acgatgcttg
    29761 gcggtcatct tcctgcacga atggcagcga aaaaagacgc cgctatcgct ttgcgtgcag
    29821 aataaggttg tgacaaccac atttagattt gagtcataag agcgctgaaa tagtagaaaa
    29881 cagcaagaaa cgaggaagta atatgttcct tgtttcgcgc tgtttttttt aattattttt
    29941 tataattctg gtatactttt ctttcctttg taccacaact cttctggcgt atacctgtct
    30001 tttccattta tgaatactgt tccatctgct tcaatcgtta cttcagtccc aatccacata
    30061 ttacgagaaa gtgcgagaag attttttctt tttcctcttc ctttttttcc aaattgcaag
    30121 gcatcggtta tctcttctgt ccaatgagat ttcacgtatt ttacttctcc gttttcaaat
    30181 gtaactttca aaatattcgc tatattttcc acttttactg ctttaggaaa tgactccttc
    30241 atttttttca ctttccttcc tccaacacta agtatgcgct ttcttttagt gataagtata
    30301 tcatagttta ttctattatc aaaaaaacgc atccattcca ttgtagaata ctttaccgaa
    30361 caagttgaat tgttttcctc tttttcacct tttactatag ataggtaaag acaactaagg
    30421 aggaatctct catgacagat caaccgtatg atttaagaaa agtcatcgaa gaactaaaaa
    30481 cacttccagg tcaatatcac gaaacagata ttgaaatcga tcctgaagca gaattatctg
    30541 gtgtttaccg ttatatcggt gctggcggta cagtaaaacg tccgactcaa gaaggaccag
    30601 ctatgacttt caacaatatc aaaggtttcc ccggcacacg tgtgaacatc ggaacaatgg
    30661 ctagtcgtaa acgtgtaggt gaaattttgc accatgatta tcgaacactt ggtcgtttct
    30721 tgaaagacgc tgtagaaaat ccaatcaaac ctgtgaaagt agacaaagca gacgctccag
    30781 cacaagaagt cgttcatcta gctaccgatg cagacttcga tattcgcaac attttgcctg
    30841 cacctacaaa tactcctgaa gatgctggtc catacatcac aatgggtgta gtcttaggat
    30901 cagatccaga aaaaacaatg agcgatgtaa cgatccatcg tatggttcta gaagataaag
    30961 atacgatcgg tatgtacatc atgcctggcg gacgccacat tggtcacttc caaaaacaat
    31021 tcgaagcctt gaataaacca atgcctatca ctatcaatat tggtttagat ccagcaattg
    31081 ccattggcac gactttcgaa ccacctacca ctccacttgg ctacaatgaa ctatggtgtg
    31141 caggagcttt gcgtaacgaa cctattcaaa tcgttgatgg tatagcagtc gatgaagtgg
    31201 gtattgctcg tgctgaattc atcattgaag cagaaatcat gccaaatgaa acaatgcaag
    31261 aagatatcaa cacgaactca ggaaaagcaa tgccagaatt tcctggctat actggggatg
    31321 ctaatccagc tgtacaagta gtgaaagtca aagcagttac tcaccgaaaa gacaatccaa
    31381 tcatgcaaac aacgattggt cccagtgaag aacacgtatc tatggccggt attcctacag
    31441 aagcaagtat tcttgccttg gtcgataaag ctattccagg aaaagtagta aatgtctaca
    31501 atcctcctgc aggtggtggt aaattgatga ccatcatgca aatccgtaaa gaaaacgaag
    31561 cggatgaagg tattcagcgt caagcagcta ttcttgcttt atcagccttc aaagaattga
    31621 agacagtcat tttagtcgat gaagatgttg atattttcga catgaacgat gtagtttgga
    31681 ctttcaacac tcgtttccaa ggagataaag atatcatcgt gatcccagga atgaggaacc
    31741 atccattgga cccatcagaa ttaccagaat ataatcctgg ctctattcgt ttccgtggga
    31801 tgagtgcaaa aactatcttt gacggtacag ttcctttcga tatgaaagaa cattttgtac
    31861 gtgcaaaatt ccaagaagta gaagattggg aaaaatattt gaaataatga atgatctgca
    31921 actgttttga catcataaaa aggagtgcgg aaaatggaac aattggcaca acggaagaaa
    31981 aaaatcgtga ttggtgtaag tggcgcttca ggcaccattt atgccatcaa cctagtgaag
    32041 aaactaaaag aatatccgat gatagaaaca cacggaatca tcagtccatg ggcaaaacag
    32101 aacttaaagc tggaatccga cttatcacta actgaattcg aaagcctgtt tgattaccat
    32161 tattcaaaca aagatttagg tgctgctgtc gccagcggat catttttgac cgatggaatg
    32221 atcatcgtac cggcaagtat gaaaacagtc gcaggtatct ctgttggttt aggcgacaac
    32281 ctgattgcac gtgctgctga tgtaacaatg aaggagcaac gaaagttagt cattgttcct
    32341 cgtgaaacgc cattaaatgc cattcacttg gaaaacttga ctaaattagc acgactaggt
    32401 gtgcaaatca taccaccaat tcctgctttt tataatcagc ctcaaacgat ccaagatctt
    32461 gttaatcacc atacaatgaa acttttggat caatttggaa ttgtaaatga gcattcaaaa
    32521 cgttggcagg gtgattaata tggaaaaaga aacgatagtt gaagtaacca aactaggttt
    32581 cacgatccaa gctgctgtta aagtaatcgg taaagactta ttgatcactt taacaggcgg
    32641 agatacacca catatcggaa cagtcactac attttctcgt gatacagaga tgcaaactgt
    32701 acgtttccct agccacgatg gtcggttcca taaagacgat gtcttagccg aaaaaatagc
    32761 tgtgatcatc aaaactgccg ttccgggaag ttgtacgatc acttccggta tccatgttga
    32821 ccatatctcc aatgcacaaa tcaacgcctc ttttccgatg tcagaagagt taggttacaa
    32881 gattttagat tggttggaaa aaatagattt ccatatcaaa gaaccagttt attataaaga
    32941 tggtgaaaaa acattatgaa agaagaggtt gtgaaatcag cgttatgacc tgagtatgag
    33001 agaaaaaaat ggaaaaatag cttctcatat tttgccgttt ttgagctaat accgagggac
    33061 agctgattga acgccgttta tcaagaggtt gtgataaagc cgcttcgttt cgagtagtaa
    33121 gaaaaatttt gtaaacatgg gtttccacat ttttgcataa atttgactta ctaccgagaa
    33181 actggctttt gaacaccgtt tatcaagagg ttgtgacacg tcttttgtca caacctcttt
    33241 tatttttgct tcactgtaac tgctacaata tcaccgattt cttttccgat gtttttacga
    33301 atatcttttc gtagtccaag gatgtagcag actgaaccat ctggattttt cacgcccata
    33361 ttcacgatac tgccttcata cggcatattg tcaaaagttg cttggacttt aactcttcca
    33421 gcaccgaatt cttttcttat atcatacgga aaaataatat atgccccgcc cttgtcgact
    33481 tcggaagcat agatttttga ttcaaaagaa taaatttccc tcattttatt tctcctattt
    33541 tttacatcta gtatagcata gcaaaaaaaa gaaggaactg tgacaaaagt cttgtcgcag
    33601 ttcctttttt cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatagt
    33661 caacaaacat gagaagctgt tttttgaaat tgctccttgt gactcagagt taaacggcag
    33721 gctcacaacc tcttccttaa acggtgttca aaagcttgca tctacggcac taagcccaat
    33781 cagatgaaaa atatggagtg ctatttttat ctgattgttc ttactgcttg atgcagagca
    33841 gctttttcac aacctcctac atggtgttca aaatattatt cgccttttgc ttctttcact
    33901 aattttgcga atgcttgttg ttggaattca gcagctaagg ctttcgtacg ttgttcttgc
    33961 tctgctttgc tcatttttgg attttcagtc attgctttta ccataccgtc ttgaacataa
    34021 gcttttgctt cactttccat cgtactttgc tgtttcttca ctaattcagc taacttggct
    34081 gcttggtccg tacttaattc tacccatgtc tctgggattt tgaatgtatt cactcggctg
    34141 tcatattcgt gattgttatc agcaatattg aaccagaatt tatacatgtc gtttttgtat
    34201 tcccaataag tagttttcgt cactttttca gcatcgccgt ctgtcttttc tactttattt
    34261 gtttcgttat ccgtaccagt tgcttttggt ttttcttgtt tttcatttgt tcggtaaaga
    34321 tacacttttt cttttccatt ccctaaagct ttatacaaaa gcatatctgc tccttgggaa
    34381 tcagcactgg aaacaatttt ttctgttttt gtttccgtta ctttttccat tccaaagtga
    34441 ttggacaaat tagcgacgat caaacttagt gaagcgacga agattagtcc gaaaatcaaa
    34501 gataaaaagg tttgccatgt ttttttagcg aacacgttcg ttaaagcaaa agcaagcaag
    34561 ctgacaatca aaactccaat aatcatgcat tatcccccgc tttcattaga acaggttctt
    34621 ttttcttcaa taaaaaggca ataataaagc caagaatccc aaagatgatc gctacgataa
    34681 acgcggcatg gtaacctgac agtgtggcat tgattgcctg atctttataa gctaatggcg
    34741 tattatccaa tacagatttg cttgggatat ttgcttttgt cacattcgtc aacacactga
    34801 tcaaaatagc tgtaccaatc gagcttgcga tttgacggaa tgtattattt accgctgtcc
    34861 catgactgat caagttattt ggcaatgcat tcataccaga agtcgttacc ggcatcatga
    34921 ccatggagat cccaaacata cggatcgcat aaaggatcac gatatataaa actggagtcg
    34981 tttttgtcaa gaaagcaaat ggtgcagttg ccgctgtcaa aatgaacatc cctgtgatgg
    35041 ctaaacgttt cgcgccgtat ttatcgaaaa tcgcacccgt cacaggcatc atagccccca
    35101 tgatcaatgc acccggcaac aacatcaagc cagaatggaa tgctgattct ccacgaatat
    35161 tttgaatata caatggtacg accatttctg cccctaccat cgccatattt gttacaccag
    35221 ctaaaacagc tgcaatcgtg aatgtagatg atttgaatac acgaagttcc aagaatggat
    35281 gttccaaatg taattgacgc catgagaaca aggcgatgaa aatcacacca acaactaaga
    35341 aaccaattac tttcaagctt ccccagccgt catctcctac actagaaaat ccgtaaagta
    35401 aactaccgaa tccaatcgtt gataaaatag cggataaaat atcaatgcta ggatttgaca
    35461 atggaataac acttttcatt aggaagaatg ctaaaaccaa gacaagaaca acgattggca
    35521 gtaccatacc aaacaagtca cgccaatggt agttatcaat gatataacca gataatgtag
    35581 ggcctaaagc aggagcaagt ccaataacga tccctgacat ccccattgct gatcctcttt
    35641 tttctggcgg gaaaatagat agcatgatat tttgcaataa tggcatcgat actcccacgc
    35701 cgcaagcttg gatcaagcga ccgattagta atgtactaaa attaggtgct gtaaagcaag
    35761 taatcgttcc gatcaaaaag atcgtcatgg cagaaagata taaatttctt gaactgaatt
    35821 tattgatcag ccatgctgtt attgggatca tgatcccgtt gactagcaag aaaccagtcg
    35881 ttaaccattg cacatcagac gctgaaatat cgaaagcttt catcaatgta ggaaaagctg
    35941 tcgtcaataa ggtctgattc aaaacggtac aaaatgtacc aattaataag acaacaacta
    36001 aaagtgtccg attatatggt tttccgtaaa tatctacggg ttggttgtta ctcatatttc
    36061 tccttctttc tgaatttttt ctgaaactac tagaatactt tagtcctaaa ttcgctattt
    36121 gtcaacctac ttgacacata agttaatacg gatataatta agggaagcaa gaggacaaaa
    36181 ggagtgacaa aaatggttgg ggcttccctt aaacgtttgt tggaaaatga tcaattggtt
    36241 gttggcatca gtgaattaag tgatatcgta ggtgtgtctc cacgtcaatt aaggtattgg
    36301 gaacaaaagg gattcattcg ttcgatcgaa gctgatgcga attgtcctag aaaatatcgt
    36361 ttacctacag tcataaaagt agaactgatc aaaaaatatt tagatgacgg atttactttg
    36421 tcaaaagctg cagaaaaggc agaaatccgc caaaagaaaa tgcaccatgt ccgtaaagtt
    36481 ttttcaaaag caatcaaaga tatcgaattg atcgacgaac gctatacgat tttacaaatc
    36541 ggtaacttca aagaagagaa tgctaagatg tacatcattc atgacaacga gacagacgaa
    36601 ttaacttaca agctccttcc tgtcgatgaa aaagctgact acgaacagtt gttaaaagaa
    36661 ctctaaacgc cactaatctc ctaagtacca agctttttaa taagtagatg ctacataaat
    36721 acttgaaaaa aggcctgttt gaacataaga atatccgaac ggtgatctgc gtttctttca
    36781 tatagaaagg aattctccat cactgttcgg atattttttt atttttttcg gttactttct
    36841 tctaatacat ctgttagcca atttgcatat tctgtttccc gattgatcgc atgcgctaaa
    36901 atcaggtagt gtccgtaatt ttcatgtttt tctttttcag taggaaagat cgtttttttt
    36961 cgctctaata gatgaagtcg tttttcttca tgaaggtcac gctgctgctg aataatttct
    37021 actaatgccg gatcattcac gtctttgacg aaatacaatt tcagaacaaa ttcgtctcga
    37081 ttaaccggta gttcattggt tggtgtatga atccactctg ccaattgttc cttcccttct
    37141 tctgttaatt cataaacttt cttttccaat ttttcacctg tgatctctac ttgatgctga
    37201 atgattcctt gctcttccat ttttgcaagt tcaggataaa tctgactatg ttttgcttgc
    37261 caaaattctc ctatttcatt tttgaacgat tgattcaatt catatcctgt catcttcttc
    37321 ttacttaata accctaacaa aatatatcga agtgtatttt ttcttgccat cagttttccc
    37381 tccttgacaa atctttaaat ccattctatc ataaatgagt aatcgaaaac atgtagttta
    37441 ttacatgttt taatttataa ggagatgctt aaacatgaaa gaatttaaag aattggaaga
    37501 ttttattgga actcacttta tctatacgta tgacaatggt tgggaatatg aatggtatgc
    37561 caaaaatgac catactgttg attaccgtat ccatggcgga atggtcaaag gccgttgggt
    37621 aaaagaccaa gaagctgata tcgttaaatt gacggacggc gtattcaaaa tcacttggac
    37681 agagccaaca gggacagacg tggcattgga ctttatgcca aatgaaaaga aacttcacgg
    37741 aatgatcttt ttccctaaat gggttgaaga acatccagaa atcactgtta ctttccaaaa
    37801 cgaacatatc gcagaaatgg aagcagctcg tgaaaaatat gaaacttatc caaaattact
    37861 tgttcctgaa tttgctacga tcacttatat gggtgatgca ggtatagaca atgaagatgt
    37921 tattagcgaa gaaccatacg atggaatgcc agatgatatc cgtagcggaa aatacttcga
    37981 tgaaaattat aaacgagtaa aataaggttg tgaaagagag gttgtgaaat cagcgttctg
    38041 acctgagtat tagagaaaga aacggaaaaa tagttcctca tgttttgccg ttttttgagc
    38101 taataccgag ggtcagctga ttgaacaccg tttatcaaga ggttgtgaaa tcagcgttat
    38161 gctttgagta ataagaacgc tagttagaaa atagcttttc atattttcta ggctagtggg
    38221 cttattaccg agaagcagcc gtttgaacac cgtttatcaa gaggttgtga aatcagcgtt
    38281 ttgacctgag tattagagaa acaaatggaa aaatagttcc tcatattttg ccgttttttg
    38341 agctaatacc gagggtcagc tgattgaaca ccgtttatca aagattgtaa caactgcata
    38401 ttcacacagt taaattagtg acttcaaaat gaaataatgc tcccagcaaa aaattaggct
    38461 gggagcatta tttcgttatt ctgttcatca aaaatcagcg ttttgattcc atttcatcac
    38521 aagttcctcc tcatttactg cttcatcttt ttgctgtgtt tgaattgtaa aaccttgtga
    38581 aagatagaaa tgaatagctg attgattttt ttgatagact gtcaaagtca gtgttttatt
    38641 ggtttctttt atttttttca ttagttgcgt accgattctt tgttgccggt attccctttt
    38701 cacaaagatt cctgcgataa actgatcaat caccccagca aaaccaataa tttcttgatc
    38761 tatttctgct aagtaaatca ctgcatctgg taaagctttc ctaacagatt ctacttgatt
    38821 ctcccaataa gttgatggaa tgaattcatg agaatccaag ttagcatgca gccaaatcga
    38881 tagaacagat tccagttctt tggcagacaa ttttgttgtt gtgcggatca ttttttctct
    38941 ccttacttat tcattgattt taaaataaaa accatttttt tgaagctctt ttacaagttt
    39001 tcgcccctca aaaaagtata tcttctcttg ttttactttt ctaataccaa aagaaacagc
    39061 aactttagga tcatcagctg aaacgattcg ataagttcca ccaggcaatt gatccagctt
    39121 gactttccca tgatggtcgc tttgtactga aaacggcaca ccatttctag taagtgtttt
    39181 tttcttatgt ttcaaaatga atgaactacc atttaccggt ttccctgacg aatcactaat
    39241 aataaatgaa aaatcaagca cttttttact taatctatac tgatagatta gtcgtccgat
    39301 aaacactaac agtatgacta cagctattat cgttcctgca agaataaacc attgccgcat
    39361 ggttctttcc tgatttcctt ttccttcttc tttttttacc gattcgttat aaggaatccg
    39421 atgtcctgtc actagcaaac gatgcgtgtt gatcatatac ggcgtacatg taagcaaggt
    39481 aactaagtct tctccttctg aaatcgttag aacagacgtg tcatctggca gtaccacttg
    39541 tatattttta acttgataag ccaatttctc ccccaaaaca gtcaaaataa acgtgtctcc
    39601 gttttctaaa cgatctaatt cggtaaataa ccgtcgatta ggtaaaccgc tgtgagcaga
    39661 gatcactgaa tgcgtcgaat ttccaccaag cgggaaagag gttccttgca agacgcctgc
    39721 tccattttca aggatcctac cattgagccg atcataaagt gaaatattta attggatctt
    39781 aggtatattg atacttccga tcagccattc ctctaattgg ctggatgttg tactttcatt
    39841 tctgttcttt tcattgaaag gatcagtacc aggattgaat ccttggtcag ttaatcgttg
    39901 ttttttctcc tccattttct ttcgctgcac ttcaatttca gctgcggtcc gtttttgtac
    39961 ttgttcgata cgtttttgat caataaaact atttaaagaa tccacataaa atggatataa
    40021 agcgatcagt aaccctacga cgatacatat taataaaaac aaacgaaaca aaagtcgacg
    40081 agactgtttt tgcttactct gcttcacatg tgtcctccct tgcaagtttt tttgttcttt
    40141 cttttattat atgggttcag gaaaaaaata gaaaatgaaa gccgatgagg ttgtgaaaaa
    40201 gctgtcctgc atcaagtaat aagtacagcc caaaaaaata gctactcata ttttttgttg
    40261 ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtgaa
    40321 aactgcgtta tgctttgagt aataagagcg ctagtcagaa aacagcttct catattttct
    40381 aggctagtga gcttattacc gagaagcagc tgattgaaca ccgtttaccg agaggtcatg
    40441 aaaaagctac tttgtttcga gtattaagaa aaattctgtt tcaaaacaag gagatgcaac
    40501 aaaacttatg tcgcatcctc tctccttaga actttcactt atgtttttct tgtaaaattc
    40561 ataaatattt tcatgaattt tctaatttct tgcctacctc tcgcatagga tatatgatga
    40621 atttatctaa agtttgcaaa ggagttcgac aattgaaaaa atatggccca tttttagcaa
    40681 tttttttcat tgctttggca tttctcatgc ctcatttatt cacacataaa ctaattttgg
    40741 gtgctgatgc cccattccat tacaatcggt tctatgatgc cgcgcaacaa attaaatatg
    40801 gggatttcca ttattttcct tccctttatg gattccagca aagtggcagg atcgtgaatg
    40861 ccgtttacgg cccatttttc gcttatttac aaggattgat cgtgttgctt gcaggtagct
    40921 ggtttcatta tcagattctt tcaaatttca ttctttatct tatttcagga ttttctatgc
    40981 tgattttact gagatatgtc aatgttcgaa attgggtaag tgtaccagta gccgttttat
    41041 tcatgagtac gtattctatt caatactggt ctatcaacca aggattcact agctggggga
    41101 cttgttttta tcctttatgt atgatcccta ttgctgattt cgtccttcat aaaaagttcc
    41161 ctgttatcaa agtcgctgtc tcagtgggac ttatgacaca gatccattta ctgtcttcga
    41221 tcatgttagc tttgatgtat atcccttttt atctttatta cttttttaac cagactgaaa
    41281 aaggaaaagc actgcttcaa ctgattaagt cggtcttgct ttattttgtt ctgacagcaa
    41341 atatttgggc aggattagct attgtttaca gtgggaataa gattcttcct ccctttgtta
    41401 acagagaaat gagtcaaaag gcgatagatc tggatggcag ttattggctc cattacccta
    41461 aggcctttcc tatattgata ttatcaggtt ttttgctttt tattttcttt caacaaaaga
    41521 acaccttatt ccaaaaaatc gtgttgtaca caagtcttgt tttctttttt ctttctacta
    41581 gcttgattcc atggacgaca ttgatcgaaa aaaatgtgcc ttttattagt ttgatccagt
    41641 ttccatttcg atttttcgtg ccatttacgc tattgcttct actttacctt gccctttcac
    41701 tgaaccagtg gtctgaacaa aagtggttcc gtcttttaag tatatttggc attcttattt
    41761 tttcagcaca gacgatccag aacttatacc agcatctgga aaagtgggaa aatgaaacat
    41821 ttgtcagtcg acatacttat ctattcgata cgccggaaga agcaaggaaa acattcttca
    41881 gcaaagatat gtctaccagc ttgttcgttt tccaaaaaac cacacctgac tatctgccaa
    41941 tctataagga gacaaaagac aataaatacg accggtataa cgaagaaata ctagaaaacg
    42001 aaacaacttt tataaaaaca catccaggcg gtcttttgac aatcaagtgg aaaaacactg
    42061 gggacaaaac tgttcatatc cctgttatcg tctacgatcg gactgtgcta cagcaaaatg
    42121 gaaaaacatt gactgattat gaagtaacag atatcgggac cccaatcgtg aagcagaaaa
    42181 aaggaatcaa cgagctcacg cttcattatc aaacgccaat ctatttctat ttcattcttt
    42241 ctcttacttt gattggctgg tttaccttgc tatgtttatt tatttatcat agatacaagc
    42301 ttttacgtgc ttaggcaaaa gggataaata catccgatca aaaaactctc caaacggtat
    42361 aatcgtttgg agagttttat tttaatcagt ttttttatgt tatatgttat attttttatt
    42421 cgttcacatc taattcgatt tccaccccgt agtgatcact gatgattgga ctatcgatcc
    42481 catcaaaaat ccgttgatat tttttaacct gcattccttc agggacgaaa ataaaatcta
    42541 tacgcaattt ttctgtattt tgttcccaac catctatttt cttttccaca gtagctgctt
    42601 ttcctttttc attcgcgaca acatacgaat cttgaagaag aaggcaactt tctcttacag
    42661 tataataacc ttgagagtct actggattat tgaaatcacc taggaaaaaa agtggtacgc
    42721 gagtttccaa aaagtagttt tccaagtttt tccactcaaa ataaaagcca ctttttttat
    42781 ctgtccacca agaaaagtgg cagctcgaga caaaaatgtc ctgatcatct atctttgttt
    42841 tcccaatcaa gatcttgcgt gtctgataat cttctttctt ttgagattgg gagacggtaa
    42901 ggacctgact ttccaatgtt tttttcgtaa gcagtgcatt tccttcttca tatttatcat
    42961 aaccgatgtg gctcatttcc caactccaaa aatactcttt tccgtgttcc tttaaaagct
    43021 gaactaaacg gtaggcaaaa ttatctttac ggataggtac ttgattcttt acctgacaga
    43081 acttcattag cttcgcaggt tctacttctt cagattccag taattggttg atttcttgta
    43141 aagcaatcac ttcataatct tctaacagga tcttatctgc taattcttgc aattttttct
    43201 ctggttctgg ctcaagccag ctatgcgtgt tgagtgtcat gaatttcatt ggttttctcc
    43261 ttgtcttcat ttcgaaacaa aagggttgtg acaaaatttt tgtcacaacc tctccagcaa
    43321 atagtgttct cctgctaact cctcagtaat aagctcaatg gaccaggagc tattttttgt
    43381 ttggctgttt ttatcacttg gtgcagggca gctttttcac gacctctcca ctaaacagta
    43441 ttcaaactgc tgcttctcgg taataagccc aaccaaacga aaaataggag gagctatttt
    43501 tgtttggctg ttcttatgac ttgatgcagg acagcttttt cacaacctct ccttacattt
    43561 taatggagcc gatcacttta ccagctgtca ctgtacctaa ttgcttgatc actacttctt
    43621 ctactttatc gtttgtaaat acgatcatga tcgttggatc tttttcttcg ttttggatca
    43681 catcaagatc catctcagct aatttcgttc ctgttcctac ttcttggcct tctttgacag
    43741 caatctcaaa agctggctga ttcatttgga ctgtatcgat tcccatatgg atcaaaactt
    43801 cgataccatc tttcgtttcc agcccaattg catgtttgct agggaaaata cttgtcactt
    43861 ttccagcgat cggcgcaaca acttctcttg atgttggaat aacagcaaag ccgtcaccca
    43921 tcattttttg tgagaatacc ggatctttta cttcagataa aggaatcact tctccagaag
    43981 ctgcggaaac tagttcttgt tctataccta agtattgctt actacctgct tgtccatttc
    44041 ctgtagaaac catttctggc tctggaatct ctgctcctga ttgcagcaga tcttcgatat
    44101 ctgatttcaa gacatctgct tttggtccat aaactgcttg gactccattg tctttgacga
    44161 tcaatcccat cgcccctgct cgtttccagg cttcttcagt ccctactttt tctcgatcat
    44221 ttacacttac gcgtaaccga gtcatacaag catcgacatc tttgatattt tcttttcctc
    44281 ccagcaaatg aatgatctgg accacttgct gatctgctgt tcctgttccg ctgttagata
    44341 caacggtttc ttcactgtca ttgtcgtaat ttccgttacg tcccggtgta gcaaagttga
    44401 attttttgat caagaaattt gctaagaaat acgtaacaac accaaaagcg atcacacaca
    44461 acacgaagtt gataagatct ccacctagac ccgctttgat agctaacggt gtccgagtca
    44521 gcaattcgat attcccaaat gaatggaccc gcaatggcaa gatatctgcc atcgcaaatg
    44581 cagccccttg gatcactgaa taaatgacat ataatggcac agcagcaaac ataaacatga
    44641 actccaatgg ttctgttaca cctgttagaa agacagccaa cgctgctgaa aaatacattg
    44701 atttatatct tgctttctta tcaggatcga cattacgata catcgcaaaa gccattccca
    44761 tcaagatacc tgaagaacca atcatttgtc ctactttgaa acgagccggc gtccagttat
    44821 gcaacacaaa ctcatattgc gacatatccc ctgcaccttt aagattcact aaatcagtgg
    44881 cccaagccaa ccataacgga tcttgcccaa atacttgtgt ccccgcctgt gcgcctgata
    44941 aaatctcata cgatccgcca agctgtgtat agttgatagg aatcgtcagc atatgatgca
    45001 atccaaatgg caatagtaaa cgttctaatg ttccaaataa aaacggtgct agaatcggtg
    45061 cgctttcttg tgattctgca atccataaac caaaattatt gattccagcc tgtacgtttg
    45121 gccacaccac agcaagaatc aatgccacga ttgttgacca taagataaca acaaacggaa
    45181 caaagcgctt tccattgaaa aatgacaaag catccggcaa ttttctataa ttatagtact
    45241 tattataagc cattgccccg acaaatccag cgatgatccc tacgaatact cccatattca
    45301 aagctggtgc ttccaataca cttgtaaaaa atcctttaac catgatcttc gtaccgaaca
    45361 gagtgtgggt aaatgcatct tcattcgcaa gcatgtcact tgtgacacca aagatagccc
    45421 cagtgatccg attgattaaa ataaatgaga tcccagctgc aaatgctccg cctgctcttt
    45481 cttttgccca acttcctcct attgctaaag caaataaaat atgaagatta ccaataattg
    45541 cccaaccaat attttcaatt actccgcccg ttgtgacaag cggcgtccaa tcaggattga
    45601 tcaagggaat tgatttccct atactgatca tcaacccagc tgccggcata actgctacca
    45661 caaccattaa agcctttcca aatttttgcc aaaactcaaa actaaatatc tttttcattc
    45721 cgtttagctc ctttacgcaa tcggtttcgt taaacgtatt atagcgtgcg ttttcttttt
    45781 tgtaaatgct ttatttttta tttttctgtc tataccattc tataaaacct ataatacgtg
    45841 tgttcccttc tataattttc tcctaaaaat ttattttttt aagctttttt ctatgaaata
    45901 ttttcttttg tctattgact tgtgcaatcg gttgcgttta ttatgaacgt ataaaacagc
    45961 tcattaggag gagaaaaatg aaacagatca aacgactatt tgctattgat ccatggaaaa
    46021 tcagcacgac acaattagat aaaaaaaatc ttcgactgca agaatcttta acaagtattg
    46081 gaaacggtta tatggggatg cgcgggaact ttgaagaaac ttactcaggg gaccaccatc
    46141 aaggtactta tttggcgggt gtctggtatc ccgataagac acgtgtcggc tggtggaaaa
    46201 atggttatcc agaatacttt ggaaaagtga tcaacgcctt gaactttatc gcgatagata
    46261 tttcgatcaa cgatcagctg atcgacttgg cttctttgga accagaagac ttttactggg
    46321 aattagatat gaaaaatggt gtattatctc gtacattcac aatcactact tctactaata
    46381 aagtacgcat ttccttcgaa cgttttctga gcattgtcaa aaaagaatca gcttatatct
    46441 atttaactat agaaatgctt gaaggtagcg gaacagtcaa actcgtatca aaattggatg
    46501 gaaatgttca aaacgaagac agcaactatg atgaacattt ttggaatgag attgatcgtg
    46561 gaactataga agaaatcggc tatttgacga ctaaaactat tccaaacaac tttgggattg
    46621 aacaatttac tgttactgca gcaatgcgcc atttcgttaa tggaaaatca gtcatccctg
    46681 actatcacga agatacttta gcattacatg gttgcttgtc tgcagaatta tctcaaggtg
    46741 agacatggtc agtggaaaaa gaaattatcg ttcttactag tagagatatt ccagaagcgc
    46801 agcaagcaga aaaagctgtc tcagaattaa cgaaccagac aaacaattat atccaagcaa
    46861 aagaagagca aacagcggct tgggcagaac gatggaagct tgccgatgtg gtcatcgaag
    46921 gagacgaaga agcacaacaa ggtattcgat tcaatttgtt ccaactattc tctacttact
    46981 acggcgaaga tgaacgattg aatatcggcc ccaaaggatt cacaggagaa aaatacggcg
    47041 gagccactta ctgggataca gaagcgtatg ctgtccctct ttatcttgct ttagccaaac
    47101 ctgaagtaac taagaatctg ctgaaatatc ggcataatca actgccgcaa gcaatccaca
    47161 acgctcagca acaaggatta aaaggcgcgc tttatcctat ggtcaccttc actggtgtcg
    47221 aatgccataa cgagtgggaa atcacattcg aggagattca ccgaaatggc gcgattgcct
    47281 atgcaatcta caattatacg aattacaccg gcgatacgga atatttgaaa aatgaaggac
    47341 tggaagtttt agcagaaatc gcacgcttct gggcagaccg tgtccatttt tcaaaacgag
    47401 caaataaata catgatccac ggtgtgaccg gcccaaacga atatgagaac aatatcaata
    47461 acaattggta tacaaatacg atcgcagctt gggtcttgcg ttacacgcga gaaaattatt
    47521 taacttatca aaaagaagct gctatcacta tcagcgaaga agaactagcc aaatggaaag
    47581 atatcgcaga gaacatgtac ttcccagttg acgaagaatt aggaatcttt gtccagcatg
    47641 acacattctt agataaagat ctcatgcctg tatccgatat tcccacaagc gatcttcctc
    47701 tgaatcagca ttggtcttgg gacaaaatcc ttagatcttg cttcatcaag caggcagatg
    47761 tactacaggg gatttatttc ttcaatgata cttttacaaa agaagaaaag caaaaaaact
    47821 ttgatttcta tgaaccaatg actgtccatg agtcttccct ttcgccaagt atccattcga
    47881 ttttagcagc agaattagga atggaaagca aagctgtaga aatgtaccag cgaacagctc
    47941 gcttggattt ggataattac aataacgaca cagaagacgg cttgcacatc acttccatga
    48001 ctggtagctg gttgtcgatt gtccaaggat ttgctcaaat gaaaactgcc catgatcagt
    48061 tgagattcgc accatttctg ccaaaaactt ggaaagctta ttctttccat atcaattatc
    48121 gcggacgttt attgttcatc tcagtcacag atgaacaagt tactttcgag ttattagaag
    48181 gttttcctct agaaatttac gtctatgaac agtcttattc tttagcggat cgtctagaaa
    48241 taacgctaag aaaggaggaa gcaaatgttt aaaggggtat tgtttgattt agacggtgtc
    48301 attaccgata ctgctgaatt ccattatcgt gcttggcgaa agcttggtca ggaaatcggc
    48361 attacgatcg accacacatt caacgaacag ctaaaaggcg tcagccgaga agactcactc
    48421 tctttgcttt tagcttatgg cggaaaagaa cactcttttt caaaggaaga atttgcagaa
    48481 ttagcgaaaa gaaaaaacga ttattattta gagatgatcc agacgatcga accaaaagat
    48541 gtcttccccg gggtcgtgcc tttactggat tctttaaaag aagaaaaaat caagattgct
    48601 cttgcttctg caagtaaaaa cgggccattc ctacttgaaa aaatgggtct tacaccttac
    48661 tttgatgcta ttgccgaccc ggcagaagca acaaacggaa aacctgcgcc agatatcttc
    48721 ctccttgcag caaaagctgt cggactaaaa gcagaagaat gtatcgggat cgaagacgcg
    48781 caagccggta tccaagccat cctctcaagt ggcgctcaac caattggcgt gggacgtgct
    48841 gaagaccttg gagaggagat cccgcttgtc cctgatacga cttttcttac gatcgattac
    48901 ttgaaagaaa agtgtcatga ccatggataa tccagagatc tccgtcgtac cagttccaga
    48961 gactgaaata aagaaaatca cattgaagaa cgactactta gaagtcgttc ttctcaatta
    49021 cggtgcccgc ctgcaccaaa tcttcgcacc agacaaagaa ggaaaatcag aaaatgtttt
    49081 attgtcttac gatcgtttcg aagatgttct aacagacaaa agcttttttg gagcaactgt
    49141 cggacctgta gccggaagga tccgggatgc ttcttggggt gagcatagat tggagaaaaa
    49201 ctgcggatca caccatatcc acggtggcac gaaaggctgg tctttccaat tttgggaagt
    49261 agaagttttc aaaactccat catcaatagg tgtggtcttt tacttaaaag atgaattttc
    49321 tacctatcca ggaccaatca ctgctacgat cacttaccgt ttaacaaaaa acgaattaga
    49381 gatgacaacc tctgctagta gccttgttga aaccatctgc aatccgacca accatgccta
    49441 cttcaacctt tcaggaaatg gaacaagaga catctatgaa catcagttaa cggtcttttt
    49501 agatggtgtg cttgaattag atcaagaaaa actgcctact ggaaattgga caaaaaaaga
    49561 aaaactgcca atcgacttta gaaaatcccc aacactgcaa gagatccttg catgttatcc
    49621 agatggacta gatgatgtat ttcttttgca tcatcctagg ctttccaaaa cttccttaca
    49681 attattcgag aagcattctg gtcgccaaat gactatagca acttctaata aaagtatggt
    49741 attattttct acgactggat tcgaagctga tttctctgtc aatggaaaac agatgcattc
    49801 aaactacgga ttagcaatcg aaccgcaaga aatccctgat atcgttcatt tccccaaatt
    49861 tggatcgatc aacctccatc ctggtcaaga acggatcagt cagacgattt atcggttctc
    49921 tgcacaataa caagagaaag gtgtttttat catggctatt accgtaaaag atgtggcgaa
    49981 aaaagccggt gttgccacct cgaccgtctc tcgtgtcatc aacgatcatc ctagtatttc
    50041 cgagacaaca aagaagaaag tccgaaaaat tatggatgaa ctaggttatg ttcctaacat
    50101 gactgcccgc aatctaggaa aacgcatttc cagtgccatc ggcgtaatct taccccctct
    50161 tgattcaaaa gagcggatcg gtaacccatt ttatcttgag atcatggaag caatcaacga
    50221 agaagcgcgg aattatgata tgactacagc aatcgctact gctaaaaatt ttgaattgct
    50281 gttagagaac gtccagcgta tgcatttaca aaaacaagta gatggtttta ttctagtgta
    50341 ttcagatagt caagatcctg tcattgatta tttataccaa aaccacatcc ctttcacttt
    50401 gatcggacag ccttatcatc atgagaacga aatcgtttat gtagataatg ataaccagct
    50461 tctaggtaaa caagcaacag aatttttgat ccagaatgga cataagaata tcctgtttgt
    50521 gacaaacacg actcatgaaa gtttgtattt cgaacgatat ttcggttatc aaaaagcaat
    50581 gatgatggct ggtcttcctg cgttatccag tgtagtacta gagcatccgg aggattatct
    50641 gtcctttgag aaaacgttga aagaaaacga agccacagca ttggtggtca ttgacgatat
    50701 cttcgccctt cgcacgatgc agttggcgca aatgtacggc tatgtcattc cagagacact
    50761 ctccgtcatt agcttcaaca actcgatctt ctcaacatta acacaccctt accttacaag
    50821 tatcgatatc gatatctccg aattaggacg tgtcgctatg caaaagctgc gcgaattgat
    50881 ccatgaagaa ttgagcagtg gcgtacgttt ggtcatccct catcgcttga tcaaaagaga
    50941 gacagtcgtt cctttagaaa acccatacta aatagctgac tcactaaata cgctgaccat
    51001 ttatcctatg gtcagctttt tatgtaaaag aagatactac ttctaacaca ttgtttatct
    51061 gtaaaaaaga gcgtgggaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat
    51121 aaacggcgga agcagaagca actccttcgg aaataagctg aaattcacaa aaatttaaaa
    51181 agcaattttc ttgagtttct tcttatttct tggagttaaa cgcttctgtc ccagcctctt
    51241 ttttccttcc ttcaattttt tacacgtcga cgattcaata aaaaaccgcc taatattgta
    51301 aagacaggtg caagaatcga gaaaaagacg aatggcaagt aatgcaacgc gctaactttc
    51361 atcgttccca taatgaatgt gccgctgact ccccacggta cgagagaatt gacggtcgca
    51421 cctgaatccg ctagtgttcg tgtcaaatag gtttttgata agccaacgtc atcaaaagaa
    51481 cgcttgaatg tttctcctgg aagaatgatg gacagatact gttctccaac gatcagattg
    51541 attccgatac agctcaaggc agtaaacccg actaaacgag aaggtcggtc catcttttct
    51601 tttaattctt gaacgatggt atcaacgatc agatatttga tcagtaaccc acctaatgcc
    51661 aatgcaagta aaatcaaact agcagaacct aacatactca tgatcccgcc tcgagaaagc
    51721 agttcatcta gctttggatc aggtgtattt gcgacataac cattcatcaa gatgtctgct
    51781 gcttttccga ccgtcaaact tttctcatgg ataaaagaaa gaacgagagc agctgtcgat
    51841 cccgctagta atgtagggat agccggaatt tttttgattg cccccccaaa taaaataact
    51901 agtggaatca atgtccaagg agagatccaa aagctcgctt tcatgttttg gatcatttca
    51961 ttgatactag ctagatcggc tgaatgactg ttatgtccta atatcgtata aaaaatcaac
    52021 gaaaggacga atgctgggaa atccgtataa cgcataccag cgatatgttc gaatagatta
    52081 actcctccaa tacccgctgc caagttggtc gtatctgata aaggcgagca gttattccct
    52141 aaaaaggcac cggaaacgac tgcgcctgta gtcattgcat cattgaatcc aagtaaatga
    52201 ccgatcccca aaaaagcaat tccaatcgtc gaaatcgttg taaatgaact gccgacagtc
    52261 actccgacaa ttgcacaaac aagaaatgct gtcggtaaaa agaaacgcac agaaacaagt
    52321 ttaaatccat aaaccataat cgtagggatg gttccagata aaatccatga agcaactaag
    52381 acaccaatca aaagaaaaat caggattgga atgatccctg gagtgattcc tttgatgatc
    52441 ccgtcatgga tctcatccca tgtgaacttt ttaaatctgc cataaaacaa caaacacatg
    52501 aaagctgcaa ggatcgggat atgtggcgag agctggaatc cgatgatcag tactcctagg
    52561 agtgccaaca acacgatcaa tacagcgata ctttctttta aagaaatatt tttattcatc
    52621 gtcgatctcc tatttcaaat gaaaaggccc tgccatgaaa aaactattca cttggcagga
    52681 cctcctacta aatcagcgac aaaacgtcgg ctgaaagact tatttttttt atttttcttc
    52741 taaacttttt tctacctcaa tcccccaaga atctttcttg aagtgattga tgatcaatgg
    52801 aataacgaac atgaccactg tgatagctgc tactaataat gcatcactca atgtcgctgt
    52861 acctaatgta gcaaatacga caacgatgat agagaagatc agcatgcagc tgactaccca
    52921 agctaatccg tttccgtttt taccaattcg gtaaggacgt tcgaattctg gatgtttctt
    52981 acgcatttta agcaatgaaa cagcaatcaa aatgtacacg atacaataaa taacagttgt
    53041 agcatttgta agtgtcaaga atacagcact tacgtcatcc attacaccat aaagcaaagc
    53101 aaataatgaa ataacgattg attgtgttag aacgacattt ctagatacgc cgtatttgtt
    53161 ttctttatgg aatccgaatt ttggtggaag gaatccttca cgagctactt ggatgattgt
    53221 ttttgatggt ccagtaaccc aagctgataa ttgtaaaagc acaccaataa ataccatgaa
    53281 gctgaagata tttccgatca cttctggtaa gcctaggatt tgacagtcaa tcaagatagg
    53341 ctgcgtgata tttgctaatt ccatttttcc atctggtacg acatttgcta caagcatcgc
    53401 attgatgaca ttcaataaaa ctaatccgat caaagcgata aatacgcctt ttgtataatt
    53461 ttttgttgcg tctttcaaac gaggcatgta aacagaactc atttcgatcc caacaaaaat
    53521 aaatgtgata ccagctaagt atttcaatga atcaatatgt tccaagtcag ggaaagcttt
    53581 tgaccatgaa aagtcaccta gatagccgtt tgagactagt ccgactttga aggctgccaa
    53641 aacacctaaa acgaacatga tgactactgg gatatatacc cctagccata caccgatgtt
    53701 cccgccgact tttgccatat cgaatttcaa gttcaagatc gtgatgatcc agtagataac
    53761 taaaatacaa cctaaaacga accagtgatt attgccaagt tctacattac caaatgtgtt
    53821 tcctaacaat ggtcctaaag tagaagcgac cattaccata cctgggaaca tttgcaccca
    53881 taatagccat gcaacgacaa atccccattt gctgcccagt gctgttttta cccataactg
    53941 aggaccgcct tcttgaggaa gcatcgtgga aagctcaccg gaaaccattg atatcggacc
    54001 ggcaaaaaac aaaactgcaa aaattgaata aaaaatcaat gtccaaccga ctgcggctaa
    54061 cgtaggaata ctccgaaccg ttgcacacag tgccattgtc agaccgataa aggtcccaag
    54121 acccatttta cttacttcag atttactcac cgttgacact ctccttgatt ttttggataa
    54181 aataacggtg tctttgaagt agtttcagca ataaacgaaa caattcagcg aatatttgag
    54241 gggattcttt gagtctattg cttgaagggt ttcttcaaag acaacctcaa aacaaaggga
    54301 tcgaagagct taggtctccc cttgctcttc agatccaaac atgcgtcaga aacagttagt
    54361 attcaagctg ttttctaaca tgattacctt gcttattttg cttcgcttgc caataattgg
    54421 tctgcgtaga ttttttccaa tttttcttga agagctgctt tgattttttc agcatattct
    54481 tcaaagtgtt cttctttgtt catgtatggt gtcattacgc tggcacgcaa tacagttact
    54541 tttccggcac ggttccattc ttcatctgag aatcctagtt ggttcacaaa ttgtaatggg
    54601 ctgttgccat agtctgggat agcaaagtca gtatgtgaag ttaagaactc attaccgtaa
    54661 atgcttcctt tcacgtatga agaataatca tatacgtcat ggttcaattt attcattgca
    54721 actaagtcat cgttgccttt ttctttgaat acatagtcta ccatattgaa atctggataa
    54781 gttaatggat gaacttcgat ttctttgtct cctactttaa atgacaagtc attcaagaag
    54841 ttgtagaaac gatgtgcacc ttcgattgat gcacccatta atttaccata tcctgtgaca
    54901 ttcaaaggta agacatggtg tgctgcccaa acgctggctg ctgttgcccc tgcttttgaa
    54961 ccttccaaga tataagcccc aagtaatgct gggatatctg cgcctttttc aaatacatat
    55021 gtcgcaaagt aagagataac gtctctcatg cggatatctt tgatgacgat accaccggca
    55081 gaatatggaa catagcccat tttatgtggg tcaatcgtta cagattctgc ttcttcgatt
    55141 gctttatatg cactgtgtac ttcttctaag atataatctt tgttttctgt aaagacattg
    55201 tatttgtaat gaacatcttt taaatcttca aatggaatga agttgttgtc ttcgtctaag
    55261 aaaatcgcac ggccgtatcc gccataagca gcatctacgt gtaagtagaa gtaaataccg
    55321 tctttttcta atacgcgacg caatgcaacg attttatcga taccgtcgat tgctccttct
    55381 tctgttgatc ctacaacacc gactacacct aggattggtg ttttttcagc tgctagacca
    55441 cgaacgattt tttcaagttc gttgatgtcc atgcgatagt tgtggtcaac tggcactgga
    55501 atcacttggt ctaaaccgat accgatgatg tcggcagctt ttagccatga atagtgttta
    55561 gtttgtggaa ccaaccattt tcctaatttt tctaaatgtt ttccgctacg tgctgagtga
    55621 gctttgattt cgtcgatttt ttctgggacg ctgtctaaca gattcatgat ttcttctgtt
    55681 gacaagttca ttaattccca atcgcttttt ccagcaacta gttcaggtgt cacttctttc
    55741 attgcaagcg gcaatgattt gatgttgcgg gcataccaaa gtccttctaa gtttgctaaa
    55801 gaaccatcag caacgatgtg gccccagcca tctttatagc tcattaattt tgcaaattcc
    55861 attcctactt cttcttccat ttggcttgtt gcaggagaag attcataggc aacgttatta
    55921 ccattccata gcatagcaaa attgtacgct agtaaagatg gcatcaatgt ttcagagttc
    55981 atatgacccc agtaacgtcc ggcattatgc caaggaacgg aatgggtacg catccgcgct
    56041 gaaatttctg atagaacatc ctttgtttta ttcacagtgt gttcaaaact agcactgctt
    56101 ttttcttctg gcgtaataat aggcatgtct tgaggcatgt agttttgacg ccagcctaaa
    56161 tgttcatcca ccaattcatt caacaaagct ttatagattt ggccgttctc agctttgtcc
    56221 ccaatgaata gggcatttag gtttagatct ttcgacaatg attcactcat agtgaaaccc
    56281 tcctaaaaaa atatttaaat tttgtcgagc cctacattac acctgttctt ctattctgta
    56341 aactaagttt tggcctgatt tttaatcata ttttaatatg tatcaattat ccgaattgtt
    56401 cacacttttg aaataattaa aaaaaacgag ggaatttaca aatgtaaact ccctcgcaaa
    56461 gattgtatta acgacaacca gcgttctatt ttaggatggg tttcaaaatt ggaatgttgt
    56521 aatagtattt ccctttttca aggtaaaaac ttactaaatt ctaatcaatt actttggcca
    56581 aaaagtaatt tttcttacct tttcgaataa ccacaaactt accatcgaat tcatcggatg
    56641 gatttatgac aaaatttaaa tcggtaacgc ggtcaccgtt aatacttata gctccgtttg
    56701 aaacatcttc acgtgcttga cgtttagatg gttcaatttt tgtggaaaca agcagttcca
    56761 cgatattttc aggtgtgctt gaaatttcaa cgttcggcat tttaccgaat ccttgcgcga
    56821 tttcttccgc atttagttct ttaatatttc cagagaacaa tgcttgcgtt attttttgcg
    56881 cttctttcaa gtcttcttca ctatgcacaa atcttgtaac ttcttctgcc aaacggcgtt
    56941 gtgcttcacg tttctcaggt tctgtctcta cttttttcgc taagtcttcg atttcttctt
    57001 gagacaagaa agtaaagaat ttcaagtatt tcactacatc gcgatcatct tgattcaacc
    57061 agaattggta gaactcaaat ggtgaagttt tcttcggatc aagccaaacg gcaccgcctg
    57121 cagtttttcc gaatttcgtt ccgtcagcct tcagcatcaa tgggatcgtt aaaccgaacg
    57181 cttctacttc tggtccttct aattttcgga ttaggtctaa tccagctgtg atatttcccc
    57241 attggtctgc tccaccgatt tgcaattgca catcgtatgt tttatgcaga tgcaaaaagt
    57301 cgatagattg cagaatttgg taagtaaact ctgtaaatga gattcctact tccaaacggc
    57361 tggcaacgat atcttttgct aacatcgtgt tgatgttgaa gtttttaccg tagtctctca
    57421 aaaattcaag taaagagatt tttgacaacc agtcgtagtt gttcacaaat gtgatatttg
    57481 catctttacc aaacaatttt ctcatttgat tggaaagtgc gtccacgtta tgttgcactt
    57541 gttccatcgt ttgcaacaca cgttctgttt tgcggccgct tgggtcgccg atcgttcctg
    57601 ttccgccgcc aatcagaata tatggatgat ggcctgccaa ttggaatctt ttcatcatca
    57661 taaatggaat caaatgtccg atatgcatcg aatcaccagt tggatcaacg ccgcaataaa
    57721 gtgaaatgct cttttcttca acgagctcgc gaagtctttc ttcgtttgtt tgctgattaa
    57781 tggcgtcgcg ccaagttaat tcatcaataa tattcatttt actgacctcc gtgtagtttt
    57841 tttatgaaag caggaacctg ctagcatgtg taattaatta tttcttcagg ataggatagg
    57901 aaataaaaaa agtcccttgg catataagcc aagggacgaa atctcgcggt accacccaat
    57961 ttgtatgtct agatatccaa catacctctc gaacatttaa cgctgtcgcg tggcaatcac
    58021 tgccaaagac tcctgaaatg taattcgctt taaagcatgt actggtttgc agcgaccacc
    58081 agctttctga aacattggct ttaacaacta cttcgtttcg atcttcgtct tgttattatt
    58141 aatatgtgtc tatcatagag cgcaactttt tctttgtcaa cagaaaaagg atattttttt
    58201 taaattcctc attttttctt ttttgaatcc gctctaattc cgaacatttt attttcaatt
    58261 attgttaaaa gaaataactt tttctgtatt ttttctgcaa tttatcgaga agtcacttta
    58321 ctttgcctct tattctttct tcgttacaat aaggaatggg acaagtaaaa aaatgaaaaa
    58381 gcaagggacg aggtatatgg aaaaaaagca aaaaattatt ttaggagcca cttctgcttt
    58441 aactatcgtt attttaattc taagtttgtt ttttgtcatc cgatcatcta caaaaaaaga
    58501 accaatcatt cctgaaaaaa aaataacaga aaaagcaaaa gaaccgacag atgataagca
    58561 acaactagtt actcaagcaa aaaatctggc tcaaagctac gattatgacc aagcgattgc
    58621 gttattggaa aaagatagta agaacggatc tactgaaaca caacagcttc ttcaaacact
    58681 aaaaaaggag aaagaacaac tgatcgactg gtctgatcct actcagatct ctcatgtctt
    58741 tttccacagc ttgatcgtgg acccgcaaaa agccttccac tctcaacagg cacaaggata
    58801 caaagattat atggtgactg ttgaagaatt caaccgttcg atcgaacaac tttatcaaaa
    58861 tgattttgtt ttagttaatt taaatgattt gatccaaaaa gacgaacaag gaaatctttc
    58921 ctttaacggg cttgcgctac ctgaaggaaa aaaaccattg gttctttcac aagatgatgt
    58981 cagctattat gaatatatgg ataattccgg ttttcctagc cgattggtcg tatcaaataa
    59041 caatgaaatc aaaaatgtct ataaagagaa agataaagaa gaaatcggtg actttgacat
    59101 ggttcctctg atcgatgcct ttgtcaagaa acatcctgat ttttcttatc gaggcgcaaa
    59161 aggtaccttg gccctgacag gttacaacgg agttttaggt tatcgaacat caaaatctca
    59221 atatggagac aatgaaaaaa caaaaaaaga gattaaagaa gctaaaaaag tggcggaaca
    59281 gttgaagaaa gacggctgga gctttgcttc ccatacatgg ggtcacttga atatgactca
    59341 agcttcacta gaagatgtaa aaaaagacaa tgaattgtgg cagcaagaag ttgcacctat
    59401 cctaggaaaa acaaatattc tgatttatcc atttggtgca gacatcagcg actggcagcc
    59461 ttattcagca gaaaatcaaa aatttgctta cttgaagagt caaggtttcg atattttctg
    59521 taatgtcgat gcttctacac cagcttgggg acaattaggt acggatttct atcgaaatgc
    59581 tcggatcaat atcgatggca ttcgttttca atctgattta gacggaaaaa atcctattct
    59641 tgatccattc atcaatgtcc atgatgtcta tgacaaagaa gcacgcgaat ctgcttgatt
    59701 ttacgtcatt tatgaaccaa aaaagccgaa ctgacatttt atcgtcagtt tcggcttttt
    59761 tatgctttat ttcttcttcg gtgcgattcc cttgatcagg acttctgtcc attctgctgt
    59821 atatggaagc agttttgcgt atatctccgg cttagaaatt ccttctacca ttgcttgaaa
    59881 atagaggatc aagaactcat ccgaatattt caaatccact tttccttctt tccgccctcg
    59941 atggaaaaga tcaagcatga tactgaaact ttcttgcgta tacgcatcca tcgcgtcaga
    60001 aaattctttt ttttcttttt ttgtatagta tgtcatcaaa tcgatataaa attgtttgtt
    60061 cactcgattg agataacgga ttttgttttc actcatggca atcagtgttt cttcaaatgg
    60121 aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa tcattcataa atgcaaaaaa
    60181 tacttcataa atcagccctt ccttgctgcc aaaatattta aaaatcgtcg ctttacttac
    60241 ctgagcgttc tcagctacct tatccattgt cactccagcg gttcctatgt ctgtgttcat
    60301 caagtcaaaa gctgcttgta aaatctcttt ctttttctct tgtgctctct tttcaaaacc
    60361 gttcatttgt agagcctccc gtaattttaa acttttataa tatatatagt tcaaatttat
    60421 tctgaggttt attataacta tctattgaga aagtatcaag ctagcaattc cttgtttatg
    60481 aattcattga cagaaattta taaaaagaat attatgtact aaaataataa aaaaagtttt
    60541 ataaaaaggt ataaaacctt tttattaaac tatttaatct tgtttagtat atatttttag
    60601 aaaaaggagt ggggttttta tggcagaaat cgtcaaagtc acacatttga aaaagagctt
    60661 tggtcgtttc gaagctttaa aagatgtctc tttcaccgta cattcaggtg aagtcgttgg
    60721 atttatcgga ccaaatggtt ctggaaaatc cacgacgatt cggacacttt tagggattat
    60781 aaaaaaagac ggcggaacgg caacgatttt tgggcaagat gtatgggaag atagtttatc
    60841 tatccatgaa cgtctttctt atgttccagg cgatatctct ttgtggggaa atttgactgg
    60901 aggagcgatc atcgatctct tcatgaagct tcatggaaaa gggagtgctc aaaaaaaaga
    60961 tgaactgatt caacgtttct ctcttgatcc taagaaaaaa gcaaaaaact attcaaaagg
    61021 aaatcgccaa aaagtgggct tgattgctgc tttggctgtt gattcggatc tctatttatt
    61081 tgatgagcct acttctggtt tagatccttt gatggaaacg attttccaag aagaagttga
    61141 aaaattgaaa gaacagggca aatccatcct actttcttct catattctca gtgaagtcga
    61201 gcgactagcg gataaagtcg tgatcatacg cgaaggaaca gtcgtagaag aaggaacact
    61261 cgatgaattg cgtcatttga ctcgttcgac catcactata gaaacagaag cagagaatag
    61321 tcacttagca caattgaacg gtgtccatga ctttgtgcaa aaaggtcacc tcgctcaatt
    61381 tgctgctgat aacgaagctt tcgatgtgat tttagctgaa gcgtccaaat taggtgtgaa
    61441 aaaatttgaa tctattccac cgactcttga ggatttattc atgagacatt atgaaggatg
    61501 aggaggaagc gaaatgactg ataaattttc acgctggtcc accctttttt ctcaaaacct
    61561 tagccgtgac tggaaaaaga tcctgatatg gctagtcggt gtcactgctt tttctggtgg
    61621 ttttgtccct gcgtttgaag aattgacttc cggacaagga ttagctggat tatacgaaac
    61681 cttgcaaaac ccggcaatga ttgcaatggt cggaccaact ccagcttcga atgctgctga
    61741 gtatacttta ggagcgatgt atgcaaatga aatgttgctt ttttgcagtt tgtttgctgc
    61801 tgtgatctct gttttacacg tggtgagcca aacgagaaaa aaagaagaac ttggtctgat
    61861 tgaacttatt cgttcctttc gagtcggacg tcaagcaaac tctttagctg tcatcgctga
    61921 aagtattctg atcaatttgc ttttagcaat atcgatcagc tgtacgatga tttttttcca
    61981 agcagattcg atcactgcag aaggtgcttg gctttttggg ctatctgtag gtagtatggg
    62041 gattttgggg agtgccgttg cactgtttgc tgcacaactg atgccaagtt cagccagttc
    62101 gaatggtctg tctttagcgt ttttaggtgg actttacctt ctacgagcaa taacagatgt
    62161 agctgattcc tccctttcga tgtggaatcc tttaggctgg tcttatttga cctatccatt
    62221 tacaactaac aactggattc ctttgcttta tttgtttttg ttcagtctaa tcttgataat
    62281 cagttcattt gttttagagg aacatcgaga tttgaacacc ggctatatcc ccgaacgtga
    62341 aggaaaaccg tatgctaaac gttctctttt atccttgcgt ggtttattat tctatctaaa
    62401 taaagggatg acaatcagtt ggcttatcac ttttggtttg atgggggcgg catacggctc
    62461 tatctacggg aatatgcaac agtttttaga aagtaacgac ttaatgaaac agatgtttat
    62521 ccaatctggc gtctcaattg aaaaaaactt tacaagcacg atcatggtcg tcttgatttg
    62581 tctagctgct attttaccaa ttgctagcat caatcgttta tttacagaag aaacccacgg
    62641 acgtatgaat cagctgttcg caacaaaaat ttctaggaac caactttatt ggaccaatat
    62701 gttactagct attctatttg ctatattggc aatcctgatt agttctggtg cactagggct
    62761 ttctgctttg gcttcgatga aggatgcgaa agacatggat ctattgatgt ttcttaaagc
    62821 cgggttcaat ttcctgcctg ccgttttagt gtttatcgga attagtggct ttctattcgg
    62881 atggcttcct cgaatgggta aacttgctta tatctactta gcttactcct ttgcactcaa
    62941 ttactttggc ggaattttgg atttaccaga atggttttcc aaaacagctg tccctagctg
    63001 ggttccacgg atgccaaacg attcttttga tctttccgtg ttccttacgt tatcatgtat
    63061 cagccttgca ctgttcgtcc ttggttctat tggttatagg cgaagagact tactagaagg
    63121 ataataaaaa agcatgtaca atttcatgct agcctccaaa tctgggtcaa aaatccaaca
    63181 tttggaggtc atttttttat ttacaagtct tctaactgct ctttatcaat gatccagact
    63241 tctcgattct ttctttttac ataaccattc tcctctaata atttaaattt tctagataaa
    63301 gtttcggggg tcgtaccgat aaatgcagca agttctttca ttttcattgg caggaaaaag
    63361 tgatccttcc ctgtcgcttt agctaagtca agcagataga cagcgactcg ttcttctact
    63421 ttttccatca tcaaaaaagt tgtctgctgc tgtacttttg ttaatttctc aacgttgatt
    63481 gctaaaagtt tcagactaag ggcgggataa tgcagtaaga gttccgagaa atctttttgt
    63541 ctcaatatgc aaatttccgt tttttctagt gcttcgccaa ataaagactc atttggaacg
    63601 cctagcaact ggctctctcc ctcatatccg cccggctcta ttactcgtaa aagctgttcc
    63661 tttccgctag cagataattg atacactttc atactgccgg aggccacgat cactaattga
    63721 ggttctccat tcggcgcaag aatctgttct cctttttcca catagtgatg tttgacaagt
    63781 gcgtgtactt tttcttgatc ttgctgtact aagtgattga acaaaggaac aaatgaaaca
    63841 cataaatgat catgactcat aatctctcct ccttctaaac agctatttct aataaagaaa
    63901 cataacttcc cttattccaa ctgataattt tcttttatca tagcataaac gtcaagcgaa
    63961 ccagatacca gcaaatagag tggatttctt tttgtccctt gctttatttt ccttcttttg
    64021 agaccttaag taattgattc ctatcaattt tttaaatatt tactttttga ataatcaaaa
    64081 acaaaccttc actcgcaagc cgagagtaag gtttgttttt cgcattgctt atttcgtgtt
    64141 gtctattgaa atttttccgc acctagcttt tgatatagtg gtgctagttc ttgtaaggtt
    64201 tcagcggtta tttgctcaac gtgttcgatt tcttgcggat agaagaatag tttacccatt
    64261 tgaaaggttc ggtctctcat cgcttttttc aactcttctt gtttctttgt atcggacaaa
    64321 tagtcagaca aagctaagtg gtcgtcaagt tcatgttctg gttgcggcca tatgtaataa
    64381 tccttgatat cgactgtttt gctccactct ttcagtatcg caagcaactg gttatacgct
    64441 gcaatgctac catatcgttc ttcgctttta taatgttggt acatcaaata gatttggtat
    64501 tgtttttgat tcaacaaaac tgctagacag gcattctcgt cttgacgaga ttcgcttcgg
    64561 tacgcgctcc aaaaatgact gcgtaaattc cagccattcg tccagctttc gattttaggt
    64621 ttgctcatgc cgtacatatc aggcaaaagt gcagctgtct ggagctgaat ctctttccat
    64681 ttttgccagt gttctttata ttctttcttg atttgttcaa tcttatctgt tggatggctc
    64741 tctttgagct gtttgaatgc gtagattggt cgatcaaaca actgataatc tgattttcca
    64801 agttccattg tttcgcctct ttccgttatc ttttgtattc tacctgaatt ttgcgatatg
    64861 gacaaataga ggttgtgaaa aagctgttct gcatcaagtc ataagaacag ccaaacaaaa
    64921 aactgcgatg agcatcaccg cagttttatt gaacttaagg ttgattgatc acatctaaaa
    64981 gcgttgaaaa taaaatggca ctagaagcag ctcctgggtc gatatgtccg attgcacgct
    65041 ctcctagata ggaagctcgt ccttttttag ctaccaagac tttggtgtgt tcttttgcct
    65101 ggtcaatgat ttcttttgtt aaattccctg ccttcaatgc ctctgtgact ggcagccaaa
    65161 catctaacat cgttttatcg tcagcttctg cctttcctcg gctttgaata cccgctgttc
    65221 cctgctcgat cacttcgcct aatttttctt gagaatcaat agaatcgacc tcttttgtcg
    65281 ctttcatcat gttcatgaat gcagatccgt ataacggacc agaagcccca ccgactttcg
    65341 atatcatcgc catagagaat actttgaatg tatcgctgat tgtttcagga tgatcggctt
    65401 gaaaagcttc ttcataagct gaaacccctc ttcccatgtt atttccatgg tctccatcgc
    65461 caatcggggt atctagatca ctgagatatt gtttgttctg attgattgcc tctgcaaatt
    65521 gcgtgagcca ttcttggatt tcttgaacgg ttagtttcat attatcaccc ttttatcttg
    65581 agatttttta ccatgaaatc gtctgtacat tgctttctaa agaatctttc cattgtgtct
    65641 ccgtcaagtc gatcagtgtt agagaaagac cttgcatatc tagtgaggtc ataaaattac
    65701 cgacttttcg gaaaactaca ttgacgcctc tttcttccaa cagagctaaa acatcattca
    65761 tgaacacgaa ctgttccatc aaaggcgtcc cgcccattcc gttgactagt acacctgctt
    65821 cttttggtaa attcgagtaa tcatctaaaa tcttagtaac aagttccttt gccagtactt
    65881 tagaaggctg catcttttct cgacgatagc ctggctcacc gtggatacca acaccatact
    65941 cgatctcatc ttctcctagt tcgaatcctg gtttccctac ttctggtaca gttgctgctc
    66001 gaagcgctag accgatcgtc ttagttgcct gtacgacctt ttctcccagt tctttcaact
    66061 cagctaggga agcaccgttt cttgctgcat cgcccacgat tttgtgtaca aggacagtac
    66121 ctgcaactcc gcgttttcca gcagtatacg tactattttc aacggcgata tcatcatcta
    66181 cgatcacact ttctacttca atatcgtcca tagcagctaa ttcctttgcc atatcgaagt
    66241 tcaatgcatc ccctgtataa ttcttaacga tcagcaaaac tccttttcca ctatccgctt
    66301 ccttaatagc cgtctgaatc tgatctggag ttggagaagt aaacacatca ccaagcaccg
    66361 ctgcacttaa catcccttcc cctacaaacc cggcatggga tggttcatgt ccgcttcctc
    66421 caccagaaac caaaccaact tgttccgctt tctgattctt ggctacaaca cgggaagatt
    66481 ctacacgatg aacaagttct ggatagcttt ttactaatcc ttccagcatt tcttcgacaa
    66541 tattgcttgg atcattcatg atttttttca atttattccc atcctttctt tccggcattt
    66601 tgtgcttctg ttaagatttg ctgagtatca tctgttatac cggcagtgat ggctgctgca
    66661 aaagctcctt ccactaaagg cgcatccacc agatgataac gttgctgctg ctcttcttcc
    66721 aacatgtctt ttgccagttc cgagttcaat accgcgctac cgatatccgc aaaaacaaaa
    66781 taactagctg catcgcttga ttcattgact gcttctacga ttttcatcgg gtctgatccg
    66841 atctcgccat ttattccgcc gagagaaaaa atttgcacct cttcggattt ttgcatctgc
    66901 tcaatcattt ctttaatacc atctgttact ttttgactat gagaaataag caaaatactt
    66961 tttttcacaa gaatcttcct ttcttttaca tagttgctaa cgattggttt cgctttcatt
    67021 aatgaacaat tcaagtatat aataaatttc taattttggg aagatatgtg tagtgcttct
    67081 tgaagtttgt tatactttaa ttaaaagaga ggagtgatcg aatgattaca acaacaacga
    67141 acagtgtaga gggaaaagcc gtagtagctt ataaagggat cgtttttggt gaggtcatta
    67201 ctgggatcca tgcatttaaa gacttggagg cggggttgcg aaatatcttt ggcggccgct
    67261 caaaaagcta tgaaaacgaa ctaatgggtg caagagaaga agcacttgcc gaaatggccg
    67321 aacgtgcaaa agcattagga gctgatgcga tcattggcgt aaaaatggac tatgaagtat
    67381 taggcagcga caatggcatg ctgatggtca catgtagtgg aacagctgtc tccacgagat
    67441 aagtagggga catctgtttt aataactaaa gagagatggg acatgtagtg gcactcaaga
    67501 attagatttt tcagtctaac ttcgaggtac tctaccttcc catcctctct tttttatttc
    67561 tttttgcgga tgattccgta agctgctgcg actaacacac ccgcccctgc taaggaataa
    67621 ctgacagaag ctgttttttc atttgttttt ggcaaagtct gacttgttga tttggatggc
    67681 tctgtcgttt gctgaactgt ttctgtcgtg cttccagagg tcactgattt ttctcctgcc
    67741 gaatgcaaaa tatcttttat tcgttggaaa ctagctgcta agtcagcttt ttttgcttca
    67801 aaatcattag caactgtttg tgcttcgctg atctttccgt ggattgcttg actggccgct
    67861 gtgatatccg cttgagtttg acttagcttt ccagaagctg tttgagcaac ctgatttaat
    67921 tgctgattga attcttttgc ttgttggatc ttcccgtcaa gatctgtttg gacagcttcc
    67981 gcttttgctt tcgcttcggc aagctttttc tcaagttgtg cacggtcatt tcccacttta
    68041 tttttaagat cagctaaatc ttgatcgatt gctgcttggt cttctttcaa tttgtttttt
    68101 gcagcttcta atgcttcttc gagttctttt cttttatttt ccaagtcttg caaatgatcg
    68161 atttggtcag aagaggtaga actatttatt gatgtatctt ctgaagatga aatcgtttct
    68221 cctgaattat ctgtcggtac attggaagtc atttctgttt ccgaggcacc gttttcagca
    68281 ccagaagagc ttatagttgt tgaaggttct gtttgagcag gttcagaagt ttccgcaaaa
    68341 gctgtctgtc ccaataagaa aatactggaa acagcaattg gccagattaa cggcttcatt
    68401 ctttttttca tcacttttcc catccttact actttttaac taatttctta ttataagcaa
    68461 aaatgtttgg aagaaccata aacacaacag gctgtttgtc taaaaaacgg tgcatctttt
    68521 atttttttgt acgttccggg cttttccatg tcccgcttcc ataacctaaa atcgttttga
    68581 ctgctggaat gaaagtcgtc acgatcgtta tcgcgttcac tatccagaac aaaagcatat
    68641 atagtggtgc gaaaagaagg tatttcaatt ttcttcgtcg atcatctaca atcagcgaag
    68701 ctagcaactg gaaaaatcct gcgaccattt caaagcagat gaacacaaaa ctgaatgtaa
    68761 aagtaatata aatctgttcg tagtttcctt gatatacata atgcccgatt aatccagcaa
    68821 aaagaactac ggaaatacaa aagaatattg accagacaat actcaatgtt tgatcaatga
    68881 agatgatcgt tcttccaata tgtttaaaag gatggagcat cacttttttg aaattcgtca
    68941 gccagacttc tgttccacct tttgcccaac gttttctttg tcgatatagc atcttcaacg
    69001 tcacaggtac ttccatgaaa aagataatct caggagcaaa aacagataac cagtcattta
    69061 actgatgatc ccaagcaata ctgatgtctt ctgtcgctcg atcttgtctg aatccgccca
    69121 catcgatcaa ggcttctttt cgatacatcg tatttgctcc actgtacgca taaagaccac
    69181 cgaaaacagc tgactgtgtt cgtttaatga tgcccacgat acttgagaat tcaaccgttt
    69241 gagattttgc aatcaatttg gtacgatttt ggacatccat atttgcagtg accgcagcaa
    69301 tatgtcttgc accagggcga atgaaataat tcacatacct aatcagtgca tctggctccg
    69361 gtaccgtgtc tgcatcattg cttaaaatca atttcccttt tgcaaaggca agtccgatat
    69421 taaacgcgtg ggcttttcct ttattttttt cgatccgaac cacacgaaga ttagcgtatt
    69481 ttttcatcag tctagctaat atttctggtg tttggtccgt tgaaccatcg tctgtcacta
    69541 atacctcata gttgtgataa ttcaatttcg tcatcaaata ctcgattgta tcttcgatca
    69601 caatttcttc attatgggct ggcaccatga tcgtgatgaa cggttcgaca ctcaagggaa
    69661 catctaccca ttctttttgc ttatgtttgt ataaaaacac ataacaccag acaccaataa
    69721 accatacaaa tccgcctaaa atcggataag aaacgagaaa aaatacataa tagacaaaca
    69781 agtatctaag aataaattac ctgtatgggt gattgagatc atttcagttc caccccattt
    69841 tctttataca aatctgcaat gaatgtcgta tctagatttt tctcttcgga gacagaataa
    69901 aaacggaccg tttctctctc ttcttttgtt ccaaaacgtt ccgtatacac ttcttcaagt
    69961 aattcttgtc tttttttcaa tttttcatct tcatattcaa aagatttatt tacttttttg
    70021 tatagatttc gattgttcca cctagtcaat acaaatgagg taatcaggaa actgaaaaaa
    70081 atcaatacaa aaaagccgat taagaaatac agcattttct tttctgataa aaaagcgtac
    70141 aaggaaaaat ccttggctct ttctggtctc gttaatggga aaaagaccca tataaaagga
    70201 agaacaacga tcatccatcc taaaagagcc actaacgttt gccaaatctt taaccagtaa
    70261 ttaccttttg aaaaatactt atctttcacc aaaattgatg caacttcttc tcttgttact
    70321 tttttcatat cctcacctca cgtttttttg ttacgacgta ggagtttacc atacatttca
    70381 tttcttttca cataaagtgt tcctaaattt ttcctaagaa aaagaacaaa acatttctca
    70441 tcagcaaatg aaaaatattt tgttcttttt atcagttgtt ctttttattt tttatctctt
    70501 aattatgttg ctctttccaa tacgacaatg cttttcattc ctctagatat cgccgtgtac
    70561 atttgtctgc gtcttctttg ctcatctcca tcgttttcaa atggatataa gattacatga
    70621 tcaaattcca gtccttttgc tagactcaaa ggaatgattt ggcagttttc taatccatat
    70681 gaattttttt taatcgattc ttgtagcgtt tgggtttcct cttctgtttg ggtgataact
    70741 gccgcttttt ctccttttag agaaaccaaa atctcagcta gtattttcaa caattcttca
    70801 gaggatcgaa aagagcggta ttcgggttct tttccttgcg gtcggatcga tacgatgtcg
    70861 atcttctctt gatccacagc ataggttttg aaaagctctg tgattgctcc gctagaacgg
    70921 taactatttc taagatcata ccgatggatc ggcagatggt agtcgtcgaa acaacgcata
    70981 atatcagcaa aagtagtgga agaattaaag attgcctgat tctcgtcacc tactaaagta
    71041 aagtgagttt ttggaaataa ttcgatcaat agacctaatt gtgccagggt ataatcttgt
    71101 acttcatcaa tcaatagata gcgaaatgct ggtacattta ccttttcaac aagtaaatga
    71161 cgaatagcta atataatgac tgcttcatca acagtacgtg tgggttgcca agcataagct
    71221 gagccatatc gtttttcata aattctttca aacaactgat gctcatccac ccatgccatt
    71281 tcttcgattt gtctggttat ttttcgatat tttttttgta aaagtttcct tgcataagct
    71341 acaatacttt gttccgaatc atcagaaatc aatttaccga ataattccaa ctgcctatct
    71401 tctgtcaacg agagtacctg attctgcaca gcaggacttt ttgcttgttt caaaagatga
    71461 ttcttccatt cgctcatcag tgcctttttc gttgcttgaa gcctatcgat caatttagga
    71521 tgagcaggaa ctttctcata tattttttcg atcgtttttt tagaaaggac tgtttttcca
    71581 tttttagtaa gtccacggat aaaattcgga tgatctaaaa acaaaggatc tgattgtttt
    71641 agattatcga taaacttttt gctacgcaaa cgttctgtct gctccgatac attttctccc
    71701 gtgatacgtt tgaaataagc ctcttcacct tctatttcct ctgctgaaag ctgtgaaacc
    71761 agctgtatga tggttagatt cagtggattt ctttcaccta atgatggtag gacattcgaa
    71821 atatagtcga taaaccgatg attaggtgat agaatcagca agtcatcgga tgtcatcgta
    71881 tctcgatata aatagagcag atacgcaata cgctgcatga tagcagaggt tttaccactt
    71941 cctgctacgc catttaccaa gatattttga cttttcacat cccgaatgat tgtgttttgt
    72001 tccgattgga tcgtagcagt gatagccttc atttcattgg tctcatcttg gcctaagact
    72061 tctaataata cgtcatcttg aatagctaca gacgtatcaa aaaacttgac caaacgatct
    72121 ctttccaaaa gaaattgtcg tctttcttta ataaccgtat cgattttatt ttgacgaacc
    72181 gtataagaag atggaccaat catgttattg taaaaaagtt cggcaattgg tgaacgccaa
    72241 tcataaacaa gattttcatc tgcatcatct gtgaaatttg ctgtaccgat ataaaaggat
    72301 tcttttggct cattatccag aaagtctaca acgacttttc caaagtaggg acttttcaat
    72361 aaacgttcga tctttgctag ctgattttct gcgtttttct gtttgatatt catctgatcg
    72421 atttctctat ttttcatttc aatcatcgaa aaagtatcca ggttatctaa gtaactgtca
    72481 aaattcaatc gaagctcgcc agccatttct ttcaacgact ggtttccttc tatttttgcc
    72541 gtatccatcg attcccgcag ttcgtttttt ttatcaatta gcttttgata cacgttcttc
    72601 agatatgctt cttctaactg tttttgtgtg ttgaccatac acattctcct ctttttttaa
    72661 agtggtggaa ccgattagtt tacctctttt aagagtcgaa tgcaagcttt ttaacgagta
    72721 tcatttgtta ggatatatca gttcttcagg tcggacagtt tgatataaag aaaatgcacc
    72781 atcaaggttt tttacgtggt agccagactg cttcaagatt ctttccgcta gatagctacg
    72841 taatccgcta tgacagctga cgatatattc ttgttggctg tctaattcat ttaatcggtc
    72901 ccgaagttca ttcaaaggga tatgtttggc ttccttgaat ttgccctttt ccaattcttc
    72961 tgctgttcgc acatctaata aaatttttcc ttttgctagt tctgtcggca attcatgcca
    73021 ttgtacggtt tcactaagcc cttctgcaat gttcattgct gcatatccta gcatgtttac
    73081 tggatctttg gctgagccaa atggcggtgc atacgtcaat tctaattccg gtaaatcgaa
    73141 gatcgtcaaa tgccctttga ttgctgttgc taaaatatcg atccgtttat ccacaccttt
    73201 tgccccaaca ccttgtgcac cataaatctc gccagtctct ggatggaaga tcaacttcag
    73261 taaaatatct gttgcttctg gataatagct tgcatgatct tttcctgata catgaatgac
    73321 ttcaaaagac agttcgtttt ctttggctgt tctttcactt aaaccggtgg atgcggcagt
    73381 cattccaaac gttcgtataa ttgctgttcc aataccgcct tgatttcttc gagaaattcc
    73441 ggaaatcgtg tccgcaactt gtctaccttg acgattggct ggtgaagcaa gagaaataag
    73501 tgcatctttt ccagtgattt gctgttttac aacgatagca tcccccaccg cataaatatc
    73561 aggaatattc gtttgataac gttcgtctac taggataccc ccgcgtatgc ctaattcgat
    73621 tccagcggtc actgctagct ggttatcagg acgaacacca atggataaaa taactaaatc
    73681 agcagggatg gtacgcccat catccagtcg caatcgtttc ccgtggtctt caaatccagc
    73741 gacagcatga gaagtaatca cttctactcc ttgatgagac aattcttttt ctataaaagc
    73801 tgccatttct tcatccagtg ttggaagaat atgtggtgct tgttcgatca ccatgacttc
    73861 taaacctctt ctttttaggt tttctgccat ttccaatccg atgaatcctg caccaacgat
    73921 cacggcacgc tttggctgtt tttctaatgc atgcatcact ttatcgatat ctggtacatt
    73981 tctaatagaa aaaacattgt cggcttctgt tatccctgga atcgatggaa caactggttt
    74041 tgcaccagga gataaaacca atgcatcgta ctgttcaatg tgtttttgac cctcatgtac
    74101 gacttctacc gttttgtttt cgggaaagat ggctgtcact tcatgatttg gacgcacatc
    74161 taaacaaaaa cgagcggata atgattctgg cgtttggaca agaagatttt cccgttcact
    74221 gatttctccc gaaagataat aaggcaagcc gcaatttgca aaagaaacat acggtccttt
    74281 ttcaaaaaca acgatttctg catcttccat taatcggcga agccgtgtag cagcagacat
    74341 tccacctgct acacctccga caataacgat tttcatctta tttccctcct actgttcgat
    74401 caaaccattg attcatgcca ccacgtacat tcactacatc ataaccagat ttctttaatt
    74461 ctttagctgc ttgtttactc ctcatccctg attgacaaat cacatagacg gtcttttctt
    74521 tgtctccatc atatctgttt atactttgta atggaacatt tttagctcct tttatatgcc
    74581 cgccccggta ttctgcaggt gttctaacat caagtaacgc tggattttcc aaaagctttt
    74641 cctttaattc tgggactgtt atcgatttta ttgattgaaa tagcaaattc attttctgca
    74701 cctcatttac atttttataa atacctcatg gggtatatta aacaggagct ttattaatat
    74761 gtcaaccaga atgactcttc atttttttca ctacttttta tatttgcata aaataccgat
    74821 aaggggtatt atctaattat aaaaatagag gagaatgtgt tatggagatt gataataaaa
    74881 atatcctgaa ccgtttgaaa cgtacagagg gacaaattcg tggtatccaa aaaatgatcg
    74941 aagacgaaaa agaatgtatg gatgtcatca ctcaattaag cgctgttcgt tctagtattg
    75001 atcgcgtaat gggcatgatc gttgcagata atctgaaaaa ttgctttgag aaacctgata
    75061 gtaatccaga agaacaagcg gctaaactag aacaagcgat caagatgatt attaaaaagt
    75121 aaaggaatga tttcaatgaa agtcgttttc catattgatg aattagaaaa atggtcagaa
    75181 acagggaaaa acgtaaaaaa cctgataaaa gcttctccag aaacgacgat tgtcgtatct
    75241 gtaaatggca tcgcgattac cggttatcta gactctgcca atgcagaatt cttagatttg
    75301 caaggagtcg tttttcacgc ttgcgccaat gctatgcgtg ctaatcatat ttcagaaagt
    75361 tctttacctg aacaagtaat cgttgtacca gcaggtgtcc ttgatttagt tgaactgcaa
    75421 tcccaaggtt atgcttatat caagccttag tcagtagagg ttgcgacaga agtgatcagc
    75481 ttcaagaaat ttcggcttat ttttcgaaga agctacttct ggaaaaccgt gaatcaaagg
    75541 ttgtgaaaac gctgtcctgc atcaagtaat aagaacagcc aaacaaaaat agcttctcat
    75601 atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc gtttaaggaa
    75661 gaggttgtga aatcagcggt ttgacctaag tattagagaa aaaaacggga aaatagctcc
    75721 tcatattttg ccgttttttg agctaatacc gaaggtcagc tgattgaaca ccgtttaagg
    75781 aagagattgt gaaaaagtcg cttagtttcg agtattaaga aaaattttgt gaaaatggat
    75841 ttctacattt ttgcataaat ttggcttaat accgagaaac tggcttttga ataccgttta
    75901 aggaagaggt tgtaaaatca gcggtttgac ctgagtatta gagaaacaaa cggaaaaata
    75961 gctgctcata ttttgccgtt ttttgagcta ataccgaagg tcagctgatt gaacaccgtt
    76021 taccaagagg ttgtgagcct accatttaac tccgaatcac aagaaataat ttcaaaaaca
    76081 acttctcctg tttttggttt ggttggctta ttatcgagaa gtagctactg ggagtctatt
    76141 tatcaaaaaa aggaatgcga cataggtcac accccttttt tattttcttt gaaacatctt
    76201 tcggtactcg acagcataca agacaaaaga ttgtaaaaga ccgtaagtga tgacggcaaa
    76261 aatgactctc acccacagcg ttggtaattc tgggaaaagc agcaaaagga tcatgccaac
    76321 atagatgaca gcagtcgcta ctttgccata ccacttcgct ccacttaatt tgatattttt
    76381 agatagcccg attaatccag ctacaaacat aaagccttct tttatcaaga atagaccaaa
    76441 caacagccaa agccacggtc gataccatgc catagacaaa atcagaaaaa gttgtgtcaa
    76501 tttatctgca aacggatcaa gcaccttccc taagtctgtt acctgattat atctccgagc
    76561 aatgaccccg tctagataat cagacaaacc tgagacaaca atgaccgctc ctgctatata
    76621 aaatgactgt ctcagcacaa aataaagata aatcggcagc aaaaaaatac ggaatatcga
    76681 taaaagattc ggaatcgttt gtatttcttt acgccagtct gtgcttaatt tcattttatc
    76741 gccctacctc ctcagcactc ttttcttttt attttagctt acacggggaa taaaaccaat
    76801 gatttcactg tttttatttt tctttacagc cactgatatt atgagctaat ttgagataat
    76861 cacttgctgt tttcatcgtt tactcttctt tcttcctgtc gtttgcgcat ttcttttact
    76921 agtactttat gagctttttt tgatcctcgc tctaggtcac gctgccgttt acgtgtttta
    76981 taatcgataa acagactgtc taaatcgtaa cctgtcgggt acagatcttc tgctgaacgc
    77041 accagacgaa cacgctgacg aggcacgtgg attatttttt tgtctaaata tacatcaatc
    77101 gtatcttcac ctatatcttc ataaactaac gcttcttttt ggtattcatt gacaaatatc
    77161 cgatcacctt ttgcaaagag ctggcgttct tttttattct ccttcctatt tgtaactgct
    77221 gtcttaggaa gaaattcttt tttctgtgtc cgataggctt tttccgccaa gtaacgctct
    77281 gcttgatgga tcaatttcat cgacatatcc attttatgag caatccacaa agcttggctt
    77341 tctcctgttt ctccaacccg caactgatat ttaggacgaa gtgcttcccg atcgaaagcc
    77401 atagcagccg gcacaaaatc ttcatgatct ccggcgaatt tcttgatttc tccataatgg
    77461 gtcgtggcaa tgatcaatgc cccttgctca tacatagatt ccatgattgc gatcgctagt
    77521 gcagcccctt catttggctc tgttccactg ccaatctcat ctaaaagtac caacgtattc
    77581 ctttttatct ttttcaaaat agcagcgatg ttttgcatat gaccagaaaa tgtactcaac
    77641 gcattttcta aattttggtg gtcaccgaca tcgacgaaaa tctcatcaaa gacagcaagc
    77701 tctgtccctt cttttgccgg aacttgcata ccgaacatcg ccatcaaagt caataaaccg
    77761 actgtcttca acacgactgt tttgcctccg gcatttgctc cggtgataat caagcctcga
    77821 tagtctttac cgatttcaac atccaatggt accgcatgct caggtaaaaa tggatgtctg
    77881 ccttgcttga taatgatatg ttccgattta ttgattctag gtgtgcttcc attgatttct
    77941 cggctaaatt ttgcccgagc aaagatgata tctaacaccg tgatcgtttc gatcaataaa
    78001 tcgatcactt cttcgttttc tgcgatagca ccagtcaatg ccgccaggac ttgatattct
    78061 tcactgattt cttcagcttt taacaactga tagtgttcat tcaatttaga aatcacagtt
    78121 ggttcaataa aaaccgtcgt tcttttattt gattcatcga taatcgttcc atctatttta
    78181 tttttatagc tggctttgat aggaatcgta tagtattcgc ctttttgaac aatcattgct
    78241 tcttggatca tttctttgtt tttcggatga cgcaagaatt tcagtaattt tgactggatt
    78301 tctttttcga ttagttgtaa ttgtttgcgt actttacgca aattacgtga cgcgtcgtca
    78361 ctgactttat tattttttat tttctggtca atcaactctt ctacatttat caaatcagga
    78421 agatgtttgc tataagcaaa caacaagggt gtctggtatt gattcttatc aaaaaacttt
    78481 gtgatcatcc gactgcttct aagaaaatcc gcatattcga tcaaatcggt tggctgcaga
    78541 atcaaccctt ttttgacttg ttctgtcagt gcatcgatcc tcggcaaccc cataaatgga
    78601 acatgctgac tgctttctaa gatcaagcgt gcttctcttg tttcttcttg acgatccaag
    78661 acagtttgta ggtttgtcga gaccgtcata tcgctgattc gttttttgct atagtttcca
    78721 attgctcttg cttggatttc ctttttgatc cgatcaaatt gtaattgact aatcatttcg
    78781 ttgtccatat tctctccttt tcttgaagag atacctgtcc caaaataaat aagggcctag
    78841 agataaataa aaggatagaa cacagcttaa aaaagtcatg tcctatcctt attagaatac
    78901 aaagatattg ttttcctgac aggtaccgtt tcataagcac accaaataaa ctttgtttat
    78961 tggctattcg acttactgat tagataccag ctacactcac aaaacatacc ccattcttct
    79021 aggtcttatt tattttccac ttaactatag caagacacat tttggttgtc aaactttagt
    79081 atgttttata gatttacacc attaatgaat agacacctac cgcgataatt gctccgatca
    79141 ttggacctgc gattggtacc catgaatatg cccaatctga agttccttta ttagctattg
    79201 gcaataccgc atgtgctaat cgaggaccaa agtcccgagc agggttgatt gcatatcctg
    79261 ttggccctcc taatgaaaga ccgatggcta agatcaatcc acctacagcg aatacgttcg
    79321 ttccaggtgc aaattcgttt tgtccaaaag ctagcaaccc aagaactaat acaaatgtac
    79381 caattaattc tgtgatcaca tttgctggat aattccgtat agcaggacct gttgcaaacg
    79441 ttcctaaaat cgcgctttca tctttcgttg cattccaatg tggcaaataa gaaagccaaa
    79501 caagaatggc ccctgcaaaa gctcctaaaa cttgcgcaat gatgaaagga acgacaagat
    79561 tccaactaat tgcccctgtc atcgccatag ctagagagac tgcaggattc aaatgagctg
    79621 ggcccatgaa tccagaaata tagacagcca ttgttacagc gagtccccaa ccaaatgcga
    79681 tgacaatcca gccagaagcc tgtgctttac ttttgtttaa attcacagcc gcacacacac
    79741 catctccaag caaaactagg atcagtgtcc caataaactc tcccatgatt tgagtcatat
    79801 cactgttcat ttatttctcc ccctttagtt cccgcagatc tgattctgca atcaatgctt
    79861 ctaaacgttt agtctgttgc gccttctcct cttctgtcca ttcaaaatat ccagcaattg
    79921 catcgatcac aggttgcttg atctcatcaa gctgatctcg ttcaaacaac agatgatttg
    79981 tacgacgaat gagataatcg cctggggcca aaaccatttc ttcttctaaa ccataacgta
    80041 accgagctga ctcagcgaga ctcaaaccag gataaggtgc catttctttt gctaattcaa
    80101 agatacgtcg ggcattagtc ccgtaaaaat cagcgatata agtagcatct tcttcctcta
    80161 aaccggctgc tactccttct tttgctaatt ctgtcactgt ttcttctaat ttagttggat
    80221 cgaagtttcc acctgaaatc tgatattttt tagagtcgat ttctttcgtc tctattccgt
    80281 attcttcttt taataattgg cgtatcaatc gcaaagctcc ttctgccatc ttacggtaat
    80341 cggtgatttt cccaccagat aaagtcagca aaccatctgg ttctctttct aaagaactac
    80401 ctctggaaat cgtagaaggt gctttttcat cacggctgtt ttccaaatgg ttcaacacgt
    80461 cttctacttc agcacgagaa actttgtttt ccttatattc acttactgta tcgacaactt
    80521 tattgaagct cttgtctgaa atcgatccat tatctccacc attgtaatca gaaccagaat
    80581 taccaatcaa cagtggacga agtcctgccc agcttgcttc gatatcattc aatgtgatat
    80641 ttgcttctgg atagcgatga ttgatcacat ccaatagata atctacatct tcttgtgtga
    80701 ctttcggatc cgtaaaatct ccttggtaat ccgtatctgt cgtaccaaag taagtcttgt
    80761 tttctcttgg aatagcaaaa accatccgcc catcttgctt tcctgtatcg aagtatgtgg
    80821 gttgcggtac aggcagtttt ttcgcatcta caactaaatg gatcccttta gttggacgca
    80881 ttttaggaga gactggacgc gtaaaattca agtttcgtac tttatctacc caaggaccac
    80941 tcgtattgat cactaatttt gctttgattt cgatcacttc atctgtcagc aggtcacgag
    81001 ctttgacgcc aacgatttga tcgccatcat ataaaaagcc aaccgctttc attttactta
    81061 ctagataagc cccatcttcg gcagcctttt tgatattatc aatcactaaa cgggcatcat
    81121 tgttgcggaa atctagatag acacccgcgc cttttagccc ttcttttttc aaaaacggtt
    81181 ctctttccaa tacttcttct ggtgtcagcg tataattctc atatttggtt cctgtcacat
    81241 ttgccagttt gtcataaagg tccattgcta ctttgaccga gaacatattg aaggttgttg
    81301 ctccttcgcc ctcatagatt ggcaaaagca ttggatctgg ttttgggatg tgtggggcaa
    81361 tactttgtac gactgcacgt tcaccaactg tgtcagctac tacttccaca tcaaatgtct
    81421 tcagataacg aataccgcca tgaacaagtt tggtcgagcg agaggaagtc ccttctgcaa
    81481 aatcctgcat ttcgatcaat cctgttttga ttcctgatgc tgctgactgg actgccacac
    81541 cggcaccagt gattccgcca ccgatgatca acagatccaa ttcttcttgc tgcatttttt
    81601 gaatgctatc ttgtcttgtc ttgtttgaaa acatatggat ttcctcctta ttcaaatgct
    81661 tgggtagctt ttactgcttt tttccaaccg ttgtaaagtt tttctcggcg ctcttcctcc
    81721 atttgaactt caaatagctt gccagcttca taaaattcgc ggatctcgtc tgtatctttc
    81781 caataaccga cagctaatcc tgctaaaaat gctgccccta gcgccgttgt ttccaagttt
    81841 tctgctcgtt ggatcggtac attcaagata tctgtttgga attgcatcag atattcattg
    81901 tttgctgcac cgccatctac tttcaataca ggaatcttga ttccagtgtc atcttgcatc
    81961 gtatcgatga tatctctcac ttgataagca atcgactgta aagtagcttt aatgatatct
    82021 tcttttgtcg taccacgtgt caagccaaac atagcccctc gtgctgcctg atcccaataa
    82081 ggagcaccta gcccaacgaa tgcaggaaca acataaacct catcttcgct tgtagagcgt
    82141 ttcgctgcgt cttctgaatc gctagctttt tgcaacattt gaagtccatc tctcagccat
    82201 tgaatcgaag aaccagccac aaaaatactt ccttctaaag catagtaaac ctttccattt
    82261 atgccgtaac caattgtcgt caataaatta tttttggata attgcggttt ttcaccggta
    82321 ttcatgacaa taaatgaacc tgtaccataa gtattcttga ccatgcctgg ttcgaatgcc
    82381 atttgtccga ataatgcggc ttgctggtct cctgccatcc ctgaaattgg tgcttcgctt
    82441 ccataaaaat gataaccttg cgtataacca taaacttctg agttagaagt gacttttggt
    82501 aacatcactc gaggaatatt tagtaaatct aaaatctcct gatcccaatc tagctgatgg
    82561 atattgaaca acatcgtccg actggcattc gagtaatcag taacatgtgc ttgtccatcg
    82621 gtcagtttcc atactaacca agaatcgatc gttccaaaca tcaattcgcc tttttcagct
    82681 cgttcttgcg cgccttctac atgatcaaga atccagcgta tcttcgttgc tgagaaatag
    82741 gcatcgatca ctaaccctgt tttttcgtgg aaaaattctt gatagccttc attttttaag
    82801 ttatctgcaa ttccagctga ctgtctggat tgccaaacaa ctgcatgata gattggacgt
    82861 cctgtatctt tttcccatat tactgttgtt tcacgctggt tcgttatgcc aatcccagca
    82921 atctgatttg gtttgatccc tgattcgatg aacgctccgg caataactga ctgtacagaa
    82981 ttccagattt catttgcatc atgttccacc caactttctt gcgggaaata ttgcgtgaac
    83041 tctttttgag aactgccgat gtgtcttcct ttcttgtcaa agataattgc tcgtgagcta
    83101 gttgtacctt ggtcaatcgc catgatatac gttgattctg tcattgtgat tccctccatt
    83161 atatgcaaac gctttcttta actactctta tcatactact gttttaaaaa aagaaactgt
    83221 caaaatttta ttcttttttt ctttttttga cagtttttta ttatgaagct attttttaat
    83281 caattctttt cttttttcag cgtctctatt cttcttttca atcgaataat gtcgtaaggt
    83341 gacgcaaatt ctgacagata atattcacac tgttttttgt ttctctggca aaatgtgatc
    83401 accaaatcat attctttacc ttcttcataa cttccaatct caataccgcc gattcccctc
    83461 agttctctga tcagatattg ttgaaaagca atacgataaa tcggcagact ttctaaatcg
    83521 attccaatca ctactggttt tgcaatataa aaatcgatca tcaaaagaac attggcatac
    83581 tggatcatca ggtaactaag ctcactatct tcagctcttt tcctcggcaa ttgctgcacc
    83641 gctgtttcct gtaatttcgc cagtaactgt gttaaggaat gcccgagcat tttctgctgc
    83701 tgttccaaaa gccgatcacg ctcatagact tcgatctttc caacaaagaa aaaccattca
    83761 ttgttgatct gggaaatttg gtagcctact gccttttctt ctttgatttt caaacgacga
    83821 ggacgataat gccataacat cgtttcccga atgtaagtat ctaaaacagc ggttggtaac
    83881 tttttgctcc gtgtcaaatc atactgataa aatatttctt cgtctactat cgaaaaagaa
    83941 acaaaaaagc tgtaaaacat cattgtttcg tatacattca gttctgctgc tgttcgacta
    84001 agatgcatca cgatgattgg atcgattgct tgataaagcc ggtccgactg atagagtaat
    84061 ttcttttctt tcaaagttgc gtacgtactt ttttcattca atagtctttt tttcgtaatc
    84121 ccgagccagc aagcaatctg tgcttctgct gatgccgtaa acgttgtttc taatacacgg
    84181 tccaatccta gaatgaacgg tcttttttct ggtgtatttt gcaaaaaaag cggtcgctga
    84241 tcttccggga tatattggaa tagttcataa taaaaatatc gtatctgtag ctcttctccg
    84301 tataattgac cgtttttgat ttgtaatcca aattcctcta agagctggtt caattctttg
    84361 atctttcgaa aaacagaaga ctcactgata ttaaacgctg tcgctaatcg aacgatggat
    84421 acttgctgat gttctaataa atagacgagc atcttaaact tcaagctttc ctgcaataaa
    84481 aacaatagaa ttttttccaa acttacatct tcgtccaaca caagcagcgc ctgatactca
    84541 tttcgctgta cttctacttt tttttccatc attcggccaa tcaattcaat atcttccaaa
    84601 tattgatcaa acgaggcttt tgacagttgg acatgctctc tcaaaccatt gatagaggct
    84661 gttccccctt gcagaacaag gtttcgcaaa atcaggatct gtaactgctc ttttctttct
    84721 aataatgctt caatcttcat aggcagtatt cgccaccttt gaccacacca tatctggcac
    84781 acggtattct ggctggaaaa atagctcatg ttcttctaac tgttcaaacg tccatccttc
    84841 ttctactgca attgtcagtt ggtcaagaag gtggaacatc tcttttttgc tttggagctg
    84901 tgctccaaga atcttttttt cagaatcaaa tagaaatttg agtttaactt tgctttgatt
    84961 tattgctgta agcttgtagt ctacgtatcc tgtttcaatt ttttggggat aaaaaatgct
    85021 ctcttcttct gtcaaaccta cgcttcctaa gaaatagcca aaccactgat tgccgactgg
    85081 acgataggtt ccctgatcct ttgtctgtga gccactaatt gtcttgctga catttcgtgc
    85141 tgtacggaca gcgttattta ctagagaaac atacattttt tcatttgttg gacgaaaagt
    85201 tacttggatc gcatcgccaa tcgcataaac gtctttttga gacgtttgca agtagtcatc
    85261 tacccagatc gttccatcat cattcaatgt taattgttct tcccacatct gattgtcggg
    85321 gcgagaatga ttagccagca aaacaagatc atagttctcc tttctttttt ccgtctctac
    85381 aatagcggtt tcattttcgg tcaaagacac aacttgttct tctaaaaaaa gtgaaagccc
    85441 ttgatttttc atttccttta ctaacggttc tgtcatttca ggatctagat aacgaaaaag
    85501 aattgacttt cggctttcgt aaagatggat ttgttttttt cttgatgcca acccttcagc
    85561 tatttcgatt cctgcttgtc ctgcaccaat aattgcaatt ttctttgcat tcatgatctt
    85621 ttgctgaaga gccgttacgt tttgtatctt tttaactggg tgaataagcg ttgattctgc
    85681 agaaatattt cgaaactttt gatttgaacc agtcgctaga atcaaacgat caaaatccaa
    85741 tttttttcct cctgctaaaa cgactttttt ttctttcaat cctattactg tcttttctgt
    85801 gtaaacatgg attcgatagc ggtcagtcag ttcttctttc gtgatccaag aaagttgttt
    85861 ttcactggta acctttcctt ttaatatcaa attgaacccg ctgggaatcc atcctaactt
    85921 cgattgcttt tcaatcaaat atatttcact tgttggcatc aattggcgaa gataaatagc
    85981 cgcatgaatc cctgcaaacg acccgccgac aattacaatg ttcatctgca ttcctctttc
    86041 ttataaggtg ttctactgct aacttctcaa tcagaaaaca cagtattcat aactcgcttt
    86101 caaccttatg atacatagcg atgcagcaga atcaaccctc tccatcatac cgtgttctag
    86161 gtctgcttct caataatgta ctagtggacc taattgaaga gtatcatttc tgaagagatg
    86221 tttcaaaaaa tgtactctta taaaagaaaa aaactcgcct tttacgtacc tctgtccaaa
    86281 taggaaacag aaaagaacga aaagacgagt tttagcgtgt gtaatgtaat agttagaata
    86341 gacacatcat ttatttttaa ttctttaatg cttcaacaac tgcttcgcct aattgatgag
    86401 cagattgagg attttgccct gtaagaagcc gcccatcaat ttgaaccact tctgaaaaag
    86461 ctgctccaga ctgatagttt gctccttttt ctttcaaagc atcttctgct aagaatggta
    86521 cttcattcgt tgtaccattt gcttcctctt cttcattact gaacccagtc actgtacgtc
    86581 cagctaaaat agattttcca gactcatctg taactggcaa caatcctaca acaccgtgac
    86641 aaacagctga gaccagccct tcatttcgat agatttgtga agcgactttc ccaagatcta
    86701 ttgcttctgg aaaatcccac attgttccgt ggccaccagc ataataaacc gctgcataat
    86761 catttgcatc cacatcatca ggacgtaagg tatttcccaa ggcccgattt ctgaaatctt
    86821 catcttcata ataggcccaa tcgatttcat ccatattcaa aacactttgc ggttctaaag
    86881 gtacatatcc gcctttagga ctgacaaaat ctgcatcgat cccatttttt actaatacat
    86941 cataaaaatg agtcagttca cttaaccaca aacctgtagg acgctcgatc ttttcgtact
    87001 tttctacatt tgttaatacg atcaatactt tcattttccc atcctctttc tataaaaatt
    87061 gcgttattga taacgtgctt tcaagaaatt atataatggg cttggcaaat aaagctcttt
    87121 atatgcttct tcaaaaggtt tcgttacaaa ttcatcctca cggttttcca ataatttctc
    87181 tgcccagtca ggatcgacga tcatttgctg tccaattgct gcaagttctg catatttcaa
    87241 tacctgctct acatcttcgc gattgcggat cccaccgaca ccaactaaag ggatctttcc
    87301 ttgaatcgtg ttatggacca cttcaagaat cgatttatcg ttgtatttat cagaacgagc
    87361 tattcgatca tacatattta atgaaacatg cagataatct agttttgact cacgtaatcg
    87421 ttcaagaagc cagatcgtgt cttcaaaacg gatacctggt gtttcatatt cttctggtga
    87481 gaaacgatac cctacgatga atggtttttt agcatattgt tcaaccgctg agaaaaccgt
    87541 tgttaataat tcttcaataa aacgataacg attttccaag ctaccgcccc attgatcctc
    87601 tcgtctattc gaatgcggag agaaaaattg ttggatcaaa tacgtatttg ctccatgcaa
    87661 ctcgattcca tcaaatcctg attcaatcgc tctacgtgtg gctttgccaa atgcttggat
    87721 tgtttcaacg atttcttctt cactcattgc tcgtggtgtt tccgcatcag gacgttttgc
    87781 tgcaactgca cttgcagata caggctgttc tccagataaa gttgctcgac ttgtcattcg
    87841 tccaccgtgg aaaatctgaa taatcgcttt tgcgccttct ccttggattg ctttcgctag
    87901 ctcacttaat cgaggaatca tttcatcatg agcgatgctg agttcacctg gccagccttt
    87961 tccgatatct tggacatttg cagcaccagt aataacagca ccaagacctt tagaacgttg
    88021 tttataataa gtgatctcat cttcggtgat cactccattg aaaaagctga gttgtgtcgt
    88081 cataggcgac agcatcaatc ggttcttcaa ttgaactcct gaaggtaaag taattgattg
    88141 atcaaattgt gacattgtca ttcctcccat aattcgctct gattaaccta tcgagaatca
    88201 tctatcatta aaccactcga ttagttgtac atgcaactaa tttgcttaaa aaattttatt
    88261 ttttttgtaa cttatctgtt gcatcattca ttgtctgggc taattcttta cttgcttcat
    88321 ttcccaaaac tgctttgtat gactgatgaa gttcttccca gctaccatcg acttccttag
    88381 caaattggcg tccttgttct gtaagataaa ctaatgacaa gcgaccttcg ctaactgttt
    88441 tgacgtatcc tttatattct aatttttcaa caaaacgagc aatcgttgat ggggcggaat
    88501 agagcatttg tgcaatatct ttttgtggta ttccatcttt ttcttttaat atcaataaaa
    88561 taaaaccata agttggtgcc agacctgtcc ttctaaacgc ttcatcagct aatttattca
    88621 acatgcgatc tagctttttc acgctaaaga aaagacattc atctaacgct ttttcaattg
    88681 gcttttccat ttcatcccct ccattggcgt acatatcata gtatgatttt tattttttgt
    88741 caaaaaacac ggaataatgt ccgcctcctc tgtctagatt tgaaaaaaag aagtagccat
    88801 cttcaaagaa tttcggcttg ttttcgacaa aactccttct ggaacaccgt gaatcagagg
    88861 ttgtgaaaaa gtcgtttagt tttgagtatg aagaaaaatt ttgtgaaaat ggctttccac
    88921 atttttgcat aaatttggct taataccgaa aaactggctt ttgaacaccg tttatatcag
    88981 aggttgtgaa aaagccgtcc tgcatcaagt aataagaaca gccaaacaaa aatagttcct
    89041 catattttgt cgttttttga gctaatgccg atggtcagct gattgtacac cgtttattga
    89101 gaggtcgtga gcctgccgtt gaactccgag tcacaaggag caatttcaaa aaacagcttc
    89161 tcatgtttta tgactatggt gacttgtgat cgaggagttg gcaggagaat actgtttatt
    89221 cgtaaagaag gggctgtgac aagacttttg tcacagtccc ttatttcctt ttattatagg
    89281 atttcgccat ttgattggat gacttgtctg taccaatcaa atgatttttt ctttaaccgt
    89341 ttctttgtgc cattcccttc gtcatcttga tcaacataga tatatccata acgttttgac
    89401 atttgtgaag tagaagcgct gacgatatca atgcagcccc acgacgtata accaaataaa
    89461 tctactccat ctttgatagc ttccttcatc tgctcaatat gctgtttcaa atagtcaatg
    89521 cggtaatcgt cttgaacggt tccatcttct tctactttgt ctatagctcc gagtccgttt
    89581 tcagctatga acagtggttt ttgataccta tcatataatt gattcaaagc aattctcaaa
    89641 cccttaggat caatctgcca gccccattca cttgatggaa gatacggatt tttaattcct
    89701 ccagttatta agtttccttg gaccccttcc caattttctg gatgagcact gactgcacta
    89761 gacatgtagt agctaaaaga aacaaaatca acagggtagg ctgctaagat ttcttcatca
    89821 cctacttcta tttttaagtt gacacctttt tcttcaaaca ttcgttgcgt atacgctgga
    89881 taatatcctc ttgcttgaac atcactaaag aacaaagaag ttctttgctg ctcaattgtt
    89941 ttttgaatat catccggatc acaagaatac ggaacaagaa gctgatagct gatcatacaa
    90001 ccgacttgtg aacctttgat aatttcatga caagcttttg tagctaaagc acttgctata
    90061 aactgatggt gtgcaccttg gtatttcgct tgttccaaat ggggatggtt ttcttcaatt
    90121 actcctacgc taacaaaagg gtgatgtttt acacagttga tctcattgaa agtcatccaa
    90181 tattttactt tatttttata gcgtttgaat actgtttcag cgaaacgaac gtaaaactga
    90241 ataagctttc gattaggcca tccaccatat tctgtaacca aatataacgg catttcataa
    90301 tgggaaagag aaacgactgg ctcaatccca tattttagac attcgtcaaa cacttgatca
    90361 taaaaaaata atcccgcttc attgggtgtt tcttcatcac catgaggaaa aattcttgtc
    90421 catgcgatag acatccgata gcatttaaaa cccatttcag caaataaacg gatgtcttct
    90481 ttaaagtgat gatagaaatc atttccgtgt cttttaggat aataatactt gctgttggga
    90541 tcctgcgccg cttttacttg ctgatgcgtc atcatatgcc attgatctgt ccatttttct
    90601 ttaggtaaat cgctgtcgaa tgtatacaca tcagacacag ataatccttt ttcatctaca
    90661 tcccatgcac cttcacattg attggccgct gtcgctccgc cccataaaaa tccttctgga
    90721 aattttttca tatcgatccc cctaaacttg ctagttttct tgtttgatta tttttcttat
    90781 gactagtaat gatatttttg aatctactac tatttgtaac aataaccatt gtggtcggat
    90841 catatccctc tttgattaat tcttgcaaat ctacttccat caataaatcg cctttacgta
    90901 ctttttttcc atcagatact gcactttgaa agtatttgcc atttaaattc actgtatcaa
    90961 taccgatatg gatcagtatc tctgctcctt cttcactgac taccccaatt gcatgtttcg
    91021 ttggaaaaac tgttgaaact tccccgttta caggactgta gatttttccg tccgttggaa
    91081 tgatcgcaaa cccttctcct aaagatccac tagcaaatgt tggatcgttt acttcagata
    91141 aatcaattat ttcacctgct gcaggagtac ttaacactat atcctttgag gattcttctt
    91201 cgatttcatc cttgtagccg aaaagaaaag ttagcataca ggaactgatc atcgctaaga
    91261 aataactagc aactagccaa acaaatcctt caccaatata aatcgggaaa gacaaaatac
    91321 ttcttgtagc aactgccaaa gcaccggctc ctgcggcacc agctactcca ccaaatatag
    91381 ctccacatat acaagcaatg taaaatggtc gtttatattt aagagtcaca ccataaacag
    91441 caggttcagt gattccaaat aatgctgtga taaatgcaga taaagataaa ctttttactt
    91501 tcttattttt cgttttcaaa aataccccaa gtactgctcc tgcttgcgca gctacggcag
    91561 tcatacatgc tggacctagc gtatcacgac cgtagaaaga aagattgttg gtcattactg
    91621 gtacaagtcc ccaatgtaac ccaaaaatca ctaacacttg ccaaaatccg ccgataacag
    91681 caccagataa aacagggcta aagttataaa cagctaaata tccagctgca atcagctgac
    91741 ttgcataatc tgcaattgga cctacaacta ataaagtaat tgggaccatc acgatgatac
    91801 aaatgaatgg tgtcaataaa tttcgaatac tttcgtgcaa gcttttgcga atcttaggct
    91861 caatataaga aagaacccat acagctaaaa ttgctggaat aaccgtactt gtataccttg
    91921 ctagaatcac gggcatccct agaaatcgca aggttatgct atcactatat gcactgacaa
    91981 tcgtaggata aacgagagct gctgcaataa ccatagcgat gaaacgatct gtattgaact
    92041 tttttgcagc agtatatgcc aaaaatatag gcaagaaata gaaaaatgca tcagctgctg
    92101 cattcaaaat gatataagtt cctgattcag tagttaataa attcaaactc gtacataagg
    92161 cgagtactcc tttgatcaaa ccacttcctg ccaagacgcc taaagtcggg gcaaaaacag
    92221 cggcgatcat gtctataaaa gagtttaaaa tgcctttttt ctcagttcct ctctttccac
    92281 tagatgcatc atcttctagt ttcatttgtt tggataatac gtcatacact tcatttactg
    92341 catttcctac tacgacttga aacattcctc cagcttcgac tacagtgatc acaccttcta
    92401 gttgctctac cttctcctta tctgccttct tattatcttt tagggtaaag cgtaaccttg
    92461 ttgcacaatg gtaaacattg ctgacatttt cttcgcctcc gacaaatcgg acaatatctt
    92521 tcgctaattg tgaatagtcc atttttactt cccccttgtt aattaatgcc gtagcaatcc
    92581 ttcttacaag ataagtatag ttcttagaaa acggtttctc cattcaatta gtacccctta
    92641 taatagcgaa cgaaactgta caatagtgtg tatggtttac ttagccgagc aaatcactca
    92701 ctgcttgaaa gaaaacaagt atatcgtttc tcttatagtc aaaggagaca aaaaaaacac
    92761 gcttgatctt cctttgctga tgtataagtg gtatctcaag ctgatactct gtgatttttg
    92821 tttctacttg gcttttgata agacagcaca acaaagtctc ggcatttaac ggaagcataa
    92881 ctaggtcaga actaggagag attagttgct gaatggaatc tatcttttta tggattttta
    92941 tttgattttc ttctttgtag taatgatcaa gcagttttgt tttctttttt tcttcaacta
    93001 tggtcagctt ttcacgcaaa atctgataaa tctcccaaga ttcaaactca tctagaacac
    93061 taggtagata aatcaaatca tcccactcaa aaataagctc ttgctcagta gtcaaaattg
    93121 catcatattc cgttattttg tttgattgat ttttgaaaag atacactggt tctatccggc
    93181 attctttgat ccacatgttc atactttgtt gaatctgttt ttttagtgta ttcaaaatac
    93241 taattggctg gttactaaca atcagtatct ccccttgttt tcgaaaagga ttcaacgctt
    93301 cggcaagata caaaacaaaa taagcgcttt cagctaaatt gggacgtaat tgaaaatgta
    93361 ttgggaagaa acgccgaatt aaataaattt ctaaaggata acgggagatg atcttttgta
    93421 caaaatgatt catggtatta tgtccggcat ccatcctcgt tttcatttgt ttgatatggg
    93481 ttagcaaaaa ttctgtttca tggaacaata acgaaacagg aagatctaaa aaacgaataa
    93541 tcgtttgctc aaatgctctt agattcttct ctatttcttt gtttttttca tcattaatca
    93601 tcaaataatt taactctaat agtcgatttt caattgcttg tttttcttga ttggaaaaag
    93661 aatagggtag ttctgatgat aacttttttg tgagggtctc taccatcgga ataaataatg
    93721 tatacctgtt tgttttttct aaaagttctt ctcttcgaag aatggtcaaa acgagatacc
    93781 gagaaaatcg tatgaagtcc tctcctgaaa caatataatt atatgatata agaacctctt
    93841 ttaatgtctt ttggacagtc ggcatgatat tggaaaaaat tgtactgatt tgttgaggca
    93901 accttgcgtg ttggatcacc gtttctgttc caacaagaga taagaaaatt ctttttgaat
    93961 tttcttcagc aatgattttt agtccttttg tcgacgaaac ttccagttct aatttaggat
    94021 tgcgctcgat ccaacgcaaa atcgttttta tttgtaatga tacggctgtt tttgaaagaa
    94081 agaaaatgtc tgctatcttt tgcatactga catacccttt ttgaaacaat aagtacgtca
    94141 caatagttga agctcggaaa tccatgctgg aatagatttt atgtttcgtc acctcttgta
    94201 tgatttgctg tttagtttca gattcaattg attctaaaaa ataacctgaa cgtttatttg
    94261 actggataat gattttcttc tcgtagatag caagcatatt tttgatttca gtttggatcg
    94321 ttttcttgtt ggtacctaaa gcttcagcta attcttcgct tgttatccaa tgattcttat
    94381 ttcttaatac tacaagaatt tggagttgtc gtttatttaa gtccatttgc ttacacctcc
    94441 tctttgttat aaacatacag gaaaaacctc tcaaatgaca atagtcactt gcagaggttt
    94501 aaaaatcatt tgaagaaaga tctatattca aggtttttac cattagactg gataatttgt
    94561 ttataatatt caaaactatc ttttttcaat ctcctaaagc ttccgctccc atcattttgt
    94621 ctatccacat aaataaagcc atatcgcttc ctcatttccc ctgttcctgc cgatacgata
    94681 tcgattggtc cccaccataa gtaaccaatg atttggcaac catcttttat tgcctcagca
    94741 atttcctgaa gatgtatttt tagatactca caacgttttg gatcgtggat tcgattcaat
    94801 tcatctggtg cttcatctag tccaatgcca ttttcaacta tgaataatgg taattcataa
    94861 cggtctgtca attcattcat gacataacga aaccctagag gatcaattgg ccaactccaa
    94921 ggttcgggag atttttcctt caaaaaggga ttatcgaaac catttgctcc gccagtatct
    94981 acgcgcattt ctgcttcttt tttatagaca gcgctacgat aataactaaa tgcaatataa
    95041 tctgccgtgt tagcttgcaa gcactgttcg tctccatcta acattactgg tgcacagtct
    95101 tcttccttcc atgtcctgta aatataagct ggataatgcc ctcggcacat gacatcggag
    95161 aaaaaccatg aattgcgttt aaattgcagt gaaccaaata cattctctgg atcacagtca
    95221 tatgcataag tcgctaatgc agaaaagcta cacataatac ccatcttagc atttggatcg
    95281 attttcttta ataattgagt agcacgggcg ttcgctagaa acatatgatg acaggcttga
    95341 taaatcactt tttttgttag tgtagcaatg ggttcaagtt tatctctagc agggaatgta
    95401 accaatccag cagctgcata aggcatacga aaagcattat tgatctcatt aaaggtcatc
    95461 caatatttca ctttcccttg gtagcgttca aagacggtct gcgcataaaa ttcaaaaaag
    95521 tcgattaatt ttcgattttg ccacccccca tactctccta ccaatgctaa gggggtttca
    95581 taatgactaa gagtaattac tggctcgata tcatatcgat ttaatgtatt gattagtctg
    95641 tcataaaata cgagaccagc ctcatttggc ttctgttcgt ctcctcttgg aaatattcgt
    95701 gcccatgaga tgcttgttcg aaaagctttt aaacctaact ccttcagtaa acgaatatcc
    95761 tcttcaaatc gatgataaaa atcaatcgct tcatgagaaa gataatggag tgattcgtct
    95821 aaagcaatct cccaaatctt tttttcttca ttccactgaa tactaggatg ctggttcatg
    95881 atcccgataa gcgtatctgt cacattaggt aatttgccgt cttccaacca tgcaccttca
    95941 cattgattag cagctactgc tccgccccat aaaaaatttt ctggaaatat ttcctgatcc
    96001 ataactatct ctcctcgcta tcaacaattt cttacttgaa gtatagctcg attcatcaaa
    96061 acaagatatt ctaactattt cggcagaaga gaagaaaaaa attgaatgct actcttcatt
    96121 tttaatattt atatataaca aatacataag acgatccccg aaagaaaaca ctcgttaacc
    96181 atctaagcaa acgatttatt ttacttccaa cacaaatgct tcataaggtc gtaaatcaac
    96241 tttgccgtct ggtaactgtt tcagaggata atttgacaca ataaccctta aagcttcacg
    96301 tggaacagtg aacgtctgct tttcactgga catattacaa ataacgagcc attgctggtc
    96361 ttcatatatg cgagaatagg caaagacatg ctcaggcgtc ttttccacta attcatagtc
    96421 tccccagacg atgatgtcat gttttctacg caaatcgatc aatttcttgt acatataaaa
    96481 aacagaatct tgatctgcta acgcttcttc tgcattgatt tctttataat ttggattgac
    96541 tcgaagccat ggaattccag tagtaaaccc agcatgttca cgggcgttcc attgcatagg
    96601 cgtcctagca ttgtctcttc ctttcgcatt gattgattcc aagatttcct cttttgtaaa
    96661 tccagaagaa atcctttcgt tatacatatt gattgtctca atatcttttg cttccgcgat
    96721 atcatcgatt gggcaattcg tcatgcctaa ctcttctcct tgatagatat aaggggtgcc
    96781 tttcatcata tggagcaaga tagcaaacat cttggcactt tcttttcgat acacatcgtc
    96841 atttccccaa cgagaaacaa ttcgcggaag atcgtgatta ttccaaaata aactattcca
    96901 tccttcatca cctaaagatg tttgccattt ggataatact tttttcagtt cagagatact
    96961 caagggtttc aaatcccatt tgtctttgcc ttcttgctga tctaatccta tgtgttcgaa
    97021 ttggaagacc atggataatt catttctttt aggattggaa taaagtttag cgatttcggt
    97081 agttgctccc catgtttcgc caacagtcat cagatttttg tcaccaaaag ttgcttgatt
    97141 catttcctga agatactcat gcagtttagg tccattactt gtgatttctt tatccggttc
    97201 tttgccaatc atatcgatca cgtccatacg aaagccccca atacctttat cgatccagaa
    97261 attcatcatt tgataaatct cttggcgaac ctgtttattt ttccaattca aatcaggttg
    97321 acgcttgcta tacaagtgca gaaaatattg ccccgttttt tcatccaact gccaagcaga
    97381 accactgaat attgagcgca atctattagg ctcttttcca ttgaccggat cacgccaaat
    97441 ataatagtcc cgataaggat tttctttatt tttacgggcc tcaatgaacc acggatgttc
    97501 atcagaagta tgattcacca ccagatccat gatgatacgg atatttcttt tctttgcttc
    97561 ttcaatcagt tcttccattt cttccatcgt accgaattct gccatgattg cttgataatt
    97621 actgatatca tacccattgt catcattcgg agactcgtat actggagaaa gccaaattgc
    97681 atcaatacct aaatacgcca gataatccaa cctggaaata atcccccgca aatctccaat
    97741 accatcttga tttgaatctt gaaaacttct tggatagatt tgataaacaa ctgaatggtg
    97801 ccaccatttt tgttccatga ttttcctcct ataaatggat gtttctattt tttgcttaat
    97861 catttatttt agtctctctc actaaaaatt gtaacgcttt caaatagcta ttgcaccttg
    97921 ttatcggtaa cagaataaaa caaaaaaaca gctgaggaaa ttccctcaac tgttttttga
    97981 aaggattgat ttatgcaggg aagaatgctg atttcacaac ttctttataa acggtgttca
    98041 ctcagctgtc ccttggtatt agctcaaaaa acggcaaaat atgaggaact attttttcgt
    98101 ttttttctct aatactcagg ccagaacact gatttcacaa cctctttata aacggtgttc
    98161 actcagctgt ccctcggtat tagctcaaaa aacggcaaaa tataagaaac tattttttcg
    98221 tttttttctc taatactcag gccagaacgc tgatttcaca acctctttat ttcacattca
    98281 atatccacat gaatcccaca aatacaaaga acagaccata catgattgga gatacttctt
    98341 ttcctctttt agctgctaac atggtaattg gatagaagat aaaacctaat gcaatcccat
    98401 ctgaaatact ataagtcaaa ggcatgccga tcaagatcaa gaatgaaggg atcgcaatct
    98461 ctaagtttcc ccattcgatt tgtcgcaaag attgtgccat caatactcct accataatca
    98521 atgctggtgc agttacttgt gaagtaacaa ctgatagtaa tggagaaaag aacaagccta
    98581 acaagaagaa aatccctgta gtaatagctg tgatacctga acgtcctcca acggcgatac
    98641 cagctgatga ttctacaaat gcaccaacag gagaagtacc aaataaggaa cccgcaagca
    98701 tagctgttga atcagaagct aaagctttcc ctacacgagg catcttgttg tctttcatga
    98761 aacctgcttg gtttgctaat ccaactaaag ttcctgctgt atcaaagaaa gtcacaagca
    98821 agaatgtcag cactacgacc cacatttgaa gagaattgat gtcccctaca tgttttaatg
    98881 caactaaaaa tgttggtttc aaacttggtg ctgcggcaac aatttcagaa ggcattggaa
    98941 tcaaacctgt gaccagacct aaaatcgttg tagctgctat cccgatgaag atgccacctg
    99001 gtacacgccg aacaagcaag atagcagtta cgactaatcc aaaaatcgtc aaccaagtag
    99061 aaccaacgtt gagtgatcct aatcctacta atgtagattc attcgaaacg atgatcccac
    99121 cttcgctcag accaaggaaa gcgataaata gaccgatacc gccagaaatc gcatatttca
    99181 aatctgctgg gatcgcatca atgatcattt cccgcagctt gaagattgtg atcaaaataa
    99241 aaatcaaaga agcaacgaat acgccggcta atgctgtttc ccatggaatc cccattccta
    99301 aacaaacaga ataagcaaaa aaagcattga tcccaagagc aggtgccgtt ccaattggat
    99361 aacgggctaa tacccccatt aaaacacagc cgatcgcact ggctaatgca gtcgcagtaa
    99421 acactgctcc ttcatccatt ccagaagcac ctaatacagt tggatttaca aataagatat
    99481 aagccattga gataaatgtt gtaaatcctg ccagtacctc acgttttacc gtggtgttca
    99541 atttttctat ttcgaagtag gaaagtattt tttccttcat cttccatccc cctaaatccg
    99601 aacattataa ataaagtccg ttaaaatttc gatttattaa taaaacaacg tgttttatca
    99661 tatactaaaa caaaacataa gtaaacaaaa aattcggatt ttaagcaaaa tccgaacgtt
    99721 ccatctgatt aaatcccttt atctcaaagt tcatgaacat tcactttgga ttacaagaat
    99781 ttctttattg tgttaataaa gtttcttcat tctagtctca aaaatcaata gtgtgatttg
    99841 attttaccat ttcgtttctg ttcttctttg atgaattctt ttgtatcgac aggattcaga
    99901 ttatttagtc cgccaaaacc tgtatcgacc atttttcccc agccttttgt cttctttttt
    99961 cctgatttga gattctttgc tcgaagcttc tcactatgtt ttgctcgccg atacttcaaa
   100021 taaccttcta atcttcccat tagatccact gtttttcctc ctttttattc aatcagacga
   100081 ctcagctttt cctataacaa aataattgcc ttcagggtca gaaaaattga aagtcaagat
   100141 gccattgtta tctgtgattt ctcctggttc ttccatgttc tcgtagatag tttcaaattc
   100201 atccgtaaaa aatactaaag aaggcggtgg tcctaatacc tctggtgaaa acttctgcac
   100261 aaatactttt gggaatacgc ctaattctaa ttcttttgag atgctgagta caatactctt
   100321 aaattcttct ggcaattcga ttgtttcaga aatttctgca ccaatttttt ctacaaagaa
   100381 tcggcaaatc atctctacgt cattgacata aagcatcaca cgtgtttttt gtatcatcat
   100441 ttgacccttc tttctatttg attttctttc ctgtctataa actgattcca ttatatcacg
   100501 aaagcgcaaa caccttgtgt ttgatactat tcaagaaaaa taaagcaata gtttctagga
   100561 ttgctttcct ccacaaactt tttttacaaa aaaaaaaaac ggcaatgaat gccgctcttc
   100621 ttatttcgat tcttttataa ttctgcttct gattcatctt tggttagtgt gtgtctaaca
   100681 ctagttacgc caaatgtttg atcgattttt tccttcaacg cttcgattgc ctgtgcgcca
   100741 cgtgccgctt gttcagcatt gatggattca atgaccagca ccgctgcttg tgtttcttcc
   100801 gtcagcattg ttctttgggc ttcacgccag ttgagacagc gacagatcgc tccttgttca
   100861 tcatagtaga tgatttcttc cggaagtgcc ggagcatcgg aatctgcacc taaaggcagg
   100921 aatgattcgc caccttgggc ttttcctaaa tgcagatttc ctgcgatctt ttcgatatct
   100981 tctccgccgc aaggtacgcc aaaagacaag gaaatactat tatagatatc tacaagcgga
   101041 ttgattggga aaaattcccg ttcttgattt gcacgtttca gtaaagcttc aatagaagaa
   101101 cgtgcacctt ttttagtctt gaatgtggta aatgcctgac gccactgagc aatgacttcg
   101161 ttttggctga atgtttcttc tgtaaggtaa tttcttgctt cttttgctgc ctcattcaat
   101221 aattgatgat acgtttcttc actttctttt gttacgtggt tatcgatacc atttacggat
   101281 aagatttcga tggttgcttc aggaaatact tcccagaaat tggtatccac tattactttt
   101341 ttcatctgtt cacacctctt gattagaata attgcttgat gaatagatag tctgtttggg
   101401 gatcatctcc catataaaaa acatgttcgc tgtatttgat aaatcctttt ttacgataaa
   101461 aagcgagcgc aacatcgttg tgttcccaaa caccgagcca aatggctgtt ttttgctttt
   101521 cttttgcttt ttcaatagct gtatctagta aaaatgtacc gataccttga tgtttaaatt
   101581 tttttcgtac atagattcgc tcgatttcta aaaaatgatc agccatttcc tcagattgac
   101641 tcgttcctgt atttagtttc aggtaacccg caggctcttg atcgatactc actaggaaaa
   101701 aatcggtttc tttatctttt agctcttctg taagtttttc tagtgcataa gcttgtgtca
   101761 agtaatcagc taaatgctcc ggtgaattat cttttccaaa tgtatctgta aatgtttcga
   101821 tactaatttg ttgtagtaag tctacatctg aaggcgtcat ttttcgaatt tcgtatttat
   101881 tcacggcttc ttcctttcaa tagatccttt tattgccttt tttgacaaaa ttccaatctt
   101941 cgctaacatt cttgcatact ttatttaaaa gatattccaa ttgttttgct tcttcgtcag
   102001 aaagaccatt taatgcgact gcattggagt agttgttttc tcgaatgatg aaaggataga
   102061 tttctgctcc ttttttagtc ggatagagtt tcttgatttt tttattttct ttatcttcca
   102121 aacgttcgat catcccattg gattctaatt tcttgattgc tcgggcagtc gttgtacgat
   102181 ctactttgat catttctgct agtttttctt ggatgattcc aggattttcg caaatacgta
   102241 ctaaataaag atactgtcct cttgttaaat cgacttcttt gaattcgata ttgctaatag
   102301 aatctagcgc tcgggcaatc attccgatgt ctcgaaggat ttcagccatt tcatcccacc
   102361 tttattttgt ttgtaagact agtttacctc tttttgttgc aaatgcaact attttcaata
   102421 aaaaaatcaa gttctttttt aataactagt aaagtatatt cttttcttct tttacgaaaa
   102481 agctcaatca caatagatct ggtttttgtt ttctaaaata agtaactgtt tcttcgttgt
   102541 tcgcttttct gatagctttg gcaactcttc tatactaaaa aatcccatgt tggaagtttc
   102601 attattctca ataaacttgg cctcttcacc gtggatcgca caagcaaaaa caagtttata
   102661 atactgaaaa agctgtggaa tatctttttg tttattcgta tcaaaaactg ctcgcaactg
   102721 agtagtttct actgttaatc ctgtttcttc atagacttct ttacgcacat tttcttcagg
   102781 agacaatcct atttcagcaa atccaccagg aagtgaccat tctttcgtcc gtttatcttc
   102841 gattagtagg accttgccat cttttttgat ataggcacga acatctactt ttggtgttgg
   102901 atgtccttct ccaatcgttg tgagcgcttt tatttcttcg aaagtttctt ttcctatttc
   102961 actgatcagt tccaatgaaa tcgtccggat ctcctcaaaa cgctctttat caaatacatc
   103021 ttttccataa tatagaccag cttgagaaag tgcgagtagt cttttgtatt gagccaactt
   103081 cttgttcatt ctcttccctt cttttttagc aaatgtatga ctatgtaatc aagtatagaa
   103141 gactggttta attacggcaa ataaatataa tgaaatctgc aataaataaa agaatgttaa
   103201 aaaactcgag aacaaaagac attcgacttc tctggggctg tgaataaatg gcgttcaaaa
   103261 gcttgcatct gcggtaataa gccccaccaa acgaaaaata tgacgagcta ttcttgtctg
   103321 gctgttctta ttaattgagg caggacagct ttttcacaac ctccccacta aacggtgttc
   103381 tcctaccaac tcctcggtca caagtcacaa tattcatcaa acatgagaag ctgttttttt
   103441 gaaattgctc cttgtgactc ggagctgaac gacaggctca cgacctctcg gtaaacagcg
   103501 ttcaaaagcc aatttttcga tattaagcca tttctttgtt acttattgtc attgatttct
   103561 ttttctaatt tatccaatag tggagtgtaa tccacttgag tatagtctgt tacatcaaat
   103621 tcatatagtg ttcctaactg gaatttgtca tattttcggt cggcgatgat ctttttgaat
   103681 gcttctttcg taatatgatt ggtccctttc gttcgatctt ccaatacgtc accatagtga
   103741 taatgatcca tgatatagct caaatgacga tctgattcac gatcacgttt gtaccgacgt
   103801 tgacataaca cttcaaagtc tgctgttagg cgaatcgtga tcacctgata tccgtatcta
   103861 tctgctaatt cttggagtcg tgctttttgc ttatcactaa aaggatactc ggacacaatc
   103921 actttctttc ctgcttccat atacaaatca agtgctttgt aatagaaagg ataaactttc
   103981 gtcctttctt gttccgcccg ttcttccaaa ttgttaaacc cgatggattc tgcatagtat
   104041 tccttgattt catcgagcgt gatggcatac atatctggaa atcgttcccg tagtttattc
   104101 ataagatatg tttttcctgt accaggacta ccagccagaa gaatgaaata ctttttcatc
   104161 tcatttattt cctaagttaa ttggactttc caagttcata aacagtccaa tcttatgctt
   104221 gacaatttct tttactgctt catttgctgg aatcacatta tggcgaagag acttgttgat
   104281 ttctgtttca ctaaaggttt ctttagctac tttcgtccag ttgactaaaa gctccgttcc
   104341 aacattgaat ttgcggatcc cgttattgat caattcagga taatcttctt ctttcacacc
   104401 tgttcctcca tggataacta gggggacatc aactactgca tcgatttctt tcagcaaggg
   104461 aatgtttact tctgtttttg atttgaattg tccatgattc gtaccgatag caatagctaa
   104521 tgcatccacg cctgtagctt ctacaaattt cactgcgtct tccggtcttg tatatacctt
   104581 gtcattttca gcgacatgga ttccttcttc tgtaccgccg attgttccaa gttctccttc
   104641 tacggaaaca ccgtgcgcat gggcatattc tactactgct tttgtcttca ggatattttc
   104701 tttgaaaggc agatgggacc catcatacat gacagaagta tatccgctgt cgattgcttc
   104761 tttgatatca tcaaagtctc gggcgtggtc taagtgcaag actgcatcaa ccatttcttc
   104821 ttctgcaata gctgtcatta cattgactaa cacatgcatg ccgatatatt tagcggtatc
   104881 gacacttgtt tgaataatga tcggcgaacc catttctttt gctgcgccta gcatagcagg
   104941 aagcatttct aggttatgcg tattaaaagc tccaactgta aaattcaact cttctgctgt
   105001 ttttgttact tctttcaatg ttgtatacat cttttattgc caccttatct ttttatttgt
   105061 ttcctttgat cgttacttgt tttgcaattt gtcgcatctc atccattttg cccatgtagt
   105121 aatcgatccc ttgatcatca ccagcacgag cagcatctgc acgcttctgg ttatagaggc
   105181 tcatcagttt tttataccca tctccgatcg tctgtttatt ttccaaactc ttttctaaat
   105241 attggatggc ttgatcgttc acatacaatt cagaaaattt aagtccaact tgttcataaa
   105301 gtttcgctaa tttatgctta tcttgggtag tggaaatctc ttgtttcaat tgtgtaatct
   105361 ctgattgaat cttttcggtc gcttctggcg atagggcttt ttcttctttt gccagaactt
   105421 ttttcggttc tttttctttt ttcttaaaaa atccaaacat cattttccct actttcaatt
   105481 aaaatgcttt caaaactggg cttaagatcc aggcatataa gagtgctgcg gctactgtat
   105541 ctacatcggt tgctgtagca ttgatgaatc ccatcgaatt aaagatcgtt actagcaaag
   105601 cgggcaataa tgtaataaag aaaccatgag caataccgcc aatgatcgct cctcggcgtc
   105661 cgccaactgc gttgccaaat atccctgctg tccctccggc aaagaaattg gttagcatac
   105721 caggtaaaat catcgctaag ccgaacatgg gcaaagtaaa catggcaata atcgtaccaa
   105781 tcgttgtcgt aatgaaacct aaaatgacag catttgggct ataaggaaag aaaactggac
   105841 aatctaaggc agggatcgca tcaggaacta atttcatagc tatccctcga aatgccggca
   105901 cgatttcacc taacagtaga cgtacaccag ctagcaatac atacacaccc actacgaatt
   105961 ggatggcttg taaaaaggca aacatgatat agttttgatc tcctgataat tcagaagcga
   106021 atggttcgcc tgcaaataaa acagtgatga tatatagcgg gaccatcacg accatgacag
   106081 acaaataagt atcttgcaga aattcgaaat gtgtcggcaa cttgatatct tcgatcgact
   106141 ttttattttc gcctttctca ccaaaaagct tagcaacacc ggcttcaaat aagtacccga
   106201 tcgtacaaaa atgacctaaa gcaatatcat ttgatccagt gattttacga atgatcggtt
   106261 gcgctactgc cggcatagca atggcgaaac aagctccgat aaaaccaccg accaagatca
   106321 atacgatccc tcgcagaccg gcaaaatagc caaagactgt cgtcatcgtt gccatccata
   106381 aaattgcttg accagttaaa aaaatgtact tccattttgt gaaacgagcc aaaataatat
   106441 tgaatacaaa aatggctaag aacgtcagtg caatatctct acctaaaccc aaatcattca
   106501 ttgcttgacc attgatagat tcaatagaag ggatgatccc ttgcatatga aatccttcag
   106561 taaagatttt cccgaaataa gttaaacttc caacgatgat gctcgaaccg gcactcaaca
   106621 cttgaaatcc taggagtgtc tttaacgtgc cagagatgac ttgtcctgct gatttcttct
   106681 gcagtagtag acccaacata gcaatcaatg caatggtgat cgatgcctga gtcaaaatat
   106741 tttcgatgat aaaattgata acgcccatgt ttttaccctc ttttcttttt gtatttagat
   106801 aactccctta tcttgcagaa ctggggtgat tttttcttca atttcatttt ttgaaacaat
   106861 atttttcaaa tagatcatcg ccgttttagg atcaactgcg aacttttcga actgcgaacg
   106921 aaaattttct gctgtgatga tcacatctgg tttcatggaa actgcggaag aaatatcaca
   106981 atggtccagt ttggcttcta ctcctaattt gttcaaaaca tcctcagcag acatctgtgc
   107041 tgcaaaacta cttcctaaac ctgcaccgca tacgaataaa atgttcaatt tgttcatctt
   107101 tgttctcccc cgtatcattt attatttctc aactactgaa aaaagcagtt gtttgaaatc
   107161 ttcgatattt gtgcattcaa tcaatcgatc caacttctct tcgttgttga tcaattcaat
   107221 tagttctttc atgatgttca gatgcgagta ggagtctact gctgccaaac aaaaaataat
   107281 ctttactgga tccatttcag gattaccgaa atcgattggt tgctcaatcg tagctacgct
   107341 gactcctaat ttattgaccc catcttctgg tcgggcatgg gctagtgcca tgtgtttgcc
   107401 aatcacgata tacggtccgt aatcattcac tgcacgaatc atcgcttcga catagcgctc
   107461 ttcgacaatg ttttctttaa ccaatggtct agcagctagt tcaatcgatt cctcccaagt
   107521 agtggctttt tctttgatca tgatattgct gtccgtcaaa atatctttta acataggctg
   107581 gatctccctt ttgttgattt ctaaattgtt tgtttcgaat aatttttcta acttgccata
   107641 gatgattcga tctactcggc caccactttc tttcatgaca tctattattt gagtgaataa
   107701 ctccgtcgat tcatgactgt taggctctaa ccgctgtagt tcttcatttc ttttcaaaaa
   107761 gtcttctata accaagatat tcgtctcagt taaaatcgga tcgacaacta aaagcggctt
   107821 atttcgataa ttcaattgaa aggtgctgaa aaccagattg acatccaatt tatctactaa
   107881 ttcgatttct tttgaactta aaactgcagt aatctcgatc tgaggaaact tttctttcag
   107941 attctctgct aacagatttc cagtagccat cccatggtta cagacaacga ctgatttata
   108001 aacataattc aagtctcgct tgattacact ggcgatcgtc gaaaagtgga tgacaagaaa
   108061 agctatctcg tcctccagta tcttgccacc aattacttct tcgatcgtag gaacaaaatg
   108121 agtgactgcg cggtagattg ggaaataatt tgttcggatg ttttctttca atggattcat
   108181 gacttggata tgattcttga ttcttacaat cagtgcagcc atgtgcttat agagattttc
   108241 gaataaagct tcttccttta gatggaaagg tacgtgtgtc tcgttctcta caaattggat
   108301 caattggatc gttagtaatt gagcttgtac ccactcgatt gaattggaga tatctctagc
   108361 attgaacgtc tcaatagtga aacgaagata atttctttct acttgcggga cattgtccaa
   108421 ttgaaattcc tcgatcatgt ggtcaataaa tgcagaaaag ctactgtctt ttatttctgt
   108481 atttttccaa ttaatggcag agataaattc atgtttcctg attcgctgga tccagatcaa
   108541 tgtaaaaagc aggatttgtt ttttataaat atcatcttct tgtttcaaca acttattgga
   108601 atagatctcc tgtatttttt tgatttccac caaaggaaaa taatggagaa agattttttg
   108661 agccggtgtt aattgtgcat cttgattcat aaagaagtcg gtcattccca gattctgatt
   108721 gataaaatca tagatcatcg tacggataga tcgttctgaa cctttataaa ccagtccttg
   108781 ctttccataa ctgacgatct ctatcccata actgaccagt gttgctcgta accgtctcat
   108841 atcttcatct aatgtacttt tagagatttg aaattcttct tcttttttgt ttagatggat
   108901 cgggattcgg ctgtaagcaa cggctaaaac tagatcaaaa atacgttcgc gccgattcaa
   108961 aaagccgtct ttcacatctt ttatttcagt ttctaatcga gcttgctttt ccttttccag
   109021 cttcaacaaa aaaactttgt tgcgaattgt acggatctcc ggaagctttt tactgctcaa
   109081 ccagatattc gcttcaagaa cttcctgacg gatcgtccgc tgactgacat tgaactcact
   109141 tgctagatgt tttgctttta ctggatattg ggctgtcatg attctgtgaa gtatttcaat
   109201 tgttcttgtt ttcatagaaa tgatctcctt ctgatttcaa gtatatggtt ccgcttacat
   109261 aataaaaagt agatatttct gccacttgaa gtggaaagaa agtaagcaaa gcaaacaaaa
   109321 aataagccat atgctttttc atctcctcaa cgaaaaagac atacgcctat tattacaaaa
   109381 attttgccca tctatacaaa aaagaggttg tgaaaaagct gtcctgcatc aagtaataag
   109441 aacaactaaa ctaaaatagt gcttcctatt tttcgtttgg ttgttcttat tatcgcagat
   109501 gcaagctttt aaacaccatt tagtggagag gttgtgacag agatgttcag ccttgagcga
   109561 taagaagcga ttttgaaaaa tcgcttccca tattttacaa aatcgtgcct tattgccgat
   109621 aggctatctc tggcacactg tttattcaga aaaaggagct gcgacaaggc ttttgtcaca
   109681 actccgttca taaatatttc ctgtcaatcc agcataatcg gctgaataaa atcggcttgc
   109741 catctttttt ctttctcaga aaaatcaaaa taccatatct gagaaaaata ctgtcgttgt
   109801 gtcttacttc ctgagatcaa tcgcccatct tttcgatcga ctgaataatc acagcatctg
   109861 aaatcaattc ggatggcaaa actattttcg tggatctgtc gataatttcc tacttcctga
   109921 atagttattt ttttagtatg atttcttaca ccgtctgctt tgtttttttg cagaactttc
   109981 tgatgttcgg caaacagctt gtctgtataa cagcttctgg ctttttcagg agtttcttct
   110041 tcccaagcgg attggatctt catgaatatc ttttggattt ccagaaactg tgctttcttt
   110101 tctttagccg ttcctgacat gcgggacaat atctccgctt tcagcctttt cttttgtctt
   110161 ttattcttga cgatagaaaa agcagaaacc cctcctactg acaaaacagc taatgcaaga
   110221 aatcccgttt gctcataact agaaggacct gtacctggac cataataccc ataagaatct
   110281 ctgccgtaag aacgactgcc aaaataactg cccccatatg aacgaccacc aaatgaacct
   110341 ctgcccgaac ttcgaccgct agatgtacct ccaccatgac cacccgcgac agcgaatcct
   110401 tgggtcggca gagcaaacaa acataggccc actaacaaaa tgattgctaa ataattgatt
   110461 tttttcattg tcttcctcgt tatcttttat tacaaaaaac tatttttatg atagccttgc
   110521 taaaaggaag agtcaataac cagatgcata aactgttttt ttagcaattt tgtcttattt
   110581 agtcattgag taacatcgga caaatgtatg tgtcggcagt ccataattcc tctatcttca
   110641 gcatgaacaa gcttgcagac aagagatctg cgcttcctcc aggagagatt tggttttcct
   110701 gacaaaagtg caacagtgct gtaaatggac gatttggtaa agcactttga ctagcagcat
   110761 gagcaagttt ttgcatttcc actaacgttt tctgatcact gcgatgcaat acgcatgtgt
   110821 catctagcga actcatcaaa actaacaaca tatgcatttg tttctgtaca tatgaatcat
   110881 tatctagctc cttagctgct tggattgcaa gcctgatatg cggataccct aatatcgctt
   110941 cttcatatgc accatttacg ccatattttt gcttcaccct atgaccatga gtggtttttt
   111001 tatgacgata ttttgtatca ggaaaagcag caagcttgct gacatcatca agtattttcg
   111061 ataaagaaaa aggctgttcc atactgattt tttgtgcaat gacactgatc aatagcccca
   111121 ttgcccaaat cgctccttta tgggtattga tgccatttgt agcaatcagc attgcctttt
   111181 ccgcttttcg cccaatctca gcaattcttt ctcttaactc ctgatcaata gcatgatgcc
   111241 atgctatttg agccatttct tcaaaaaaag gagttagcgt cttagcagac ttaagaaata
   111301 aagcatagtc catatcggaa tgtgcacccg catccagtgc atccactaat ccaggttttg
   111361 gacttaatgt tacttctgcg atcaatgctt cctctaccaa cgcagcgagt tgctgagtca
   111421 ctgctttttt tcgttcgtca tttatcatga cactcactcc ttcttcctac tattacttgc
   111481 tgaaaccttt tttgatagct acccaaatga tatgaagtaa ccagcttaaa gcaaatgcac
   111541 aacttgccgt cattagataa gctacaagaa tcatgactcc tgaaggtaac tgcagccaac
   111601 tatctttaaa caataataac agtacttgga aaacaaatac attggctaaa tacgtacgat
   111661 aagcataaac agacaaccat ttgaacaatg gcaattttgg cgaatcgttt acaatcattt
   111721 tggaagcgcc tatgaagaca gcaaagatga tgactaaact gtataaactt tggatcgtat
   111781 tcaagtaagg tgcattcgca aagctcaagt ctcctggatg tgcgagcaat gaacggacag
   111841 aaaagaacgc gataactaag gcaacaggta agaaaagata acgtactctg tccaaatatt
   111901 gatagacttt ttcgtgataa acaaatgcgg ctaccccata aatcatgaaa ctagcaacaa
   111961 accgatctaa taagtaccag ctgtcatgat aaggcccagt caatacttgt gtttgataga
   112021 acagatacca tgcgacatat aaaatcgttg aaccaatcaa tactggccat acatattttt
   112081 tattagcaaa tactttgtaa ccgagccaaa caaaaaacgg catgaacaat tggatctgca
   112141 gcatcataac ggtataccat aaatgaggcg ctccatcacc tgtgacatat ttcagtaaaa
   112201 agcttgcagt atccgtatac ggagtttgtt gttggactgc tggaaatagc caaagataag
   112261 ccgtcgtcca tagaagatac ggaacgacta gttcaaaaaa cttttctttc aaaaattccc
   112321 aataagacaa atgctgactt gtttttacca tattataaac aattgcaaaa ataaacattg
   112381 gtgctgtgta tttaacgatg acgtaaaagg taccaagaaa agctgtattt gttgcgctgt
   112441 ggctgtttgc caaagcaaag ctcaatatcg tttgcatcat aacagccgtt acagctgata
   112501 ctttctggaa gtcacttacg ttgattttga tttttttgtt ttcaaacatc tctttctctc
   112561 tctttttctt tattaccaaa gttctttggt taatttaggt ccttcgactg ttttcaatag
   112621 aatttttttc tctggctctc tcaaatactc ttttagtgaa aaaccgcctt gccgagtcac
   112681 tacttgggta tcgaattgct caaaaaaagc aggatgcgat tgaattacct gaattggcaa
   112741 ttcaatcggt gaatttgcgt acagcagcaa atcaaaatca ctggtttctt ttactgccgg
   112801 aatcccagtc gataattcaa atcctaagct gccgccgatt ccccatttac atcctgataa
   112861 tatgtttcta gcagcatgaa attgctgata aacaggataa tccatgattt tctcttgtct
   112921 atacgattct tgttcgatga tgtcccacgg atgaatgact tgtttgacgg catgttttga
   112981 gatttctgtt gaccatctct gattgcgggc aggtccacgt aaaccaatcg gtagtatttc
   113041 tgcgtgatcc gtcgacgcac gacgaactgt tccgaacatc ttttcagtta gaacgaccca
   113101 ttcaggcaag gtgaaccctt ctatttcatg tttatcaaaa aggacaatat catgtgcttg
   113161 aatcattttt tatgcatccc attcttcgtc cattttggca cggactttat ttgtttcgac
   113221 acgtccgaat gttttggctg tttcgtttgt gtatcgattt gtcagcatcg tgtcatttgt
   113281 attgcggaca tcttgaatgg cttcattgat tgcttcgatg actttgctga cattttgttc
   113341 tgaagcagct tggtcggtga tatcttctag gaaatgatag atcgctccta gttttgtata
   113401 attattgata tcgtaagcga tagctggaac tttttcagca gctgcttcga tttcagcaac
   113461 cgaacgtttt gtcacacgtg cagcactttc tttagacatc gcttggacgg taatttcttt
   113521 attgtttaaa gcaatcaagc ggcttgattg taatccatgt gctaaaaatg cacctgaaat
   113581 agcatttcct gcaatcaatc caataactgg atgtccagct tgacggagtt ttgcataagc
   113641 agcagcacta ttagccaaag ccacatggat accgatcaat tcttcgttat agccgtatgc
   113701 ttggcttggt acatcgatca caacgatgat tggccgtttc acttttttgt ctttatcttc
   113761 ttcgtaaacg tgatccaaaa tttgtcccat gcggaaacct tcaactaaac caacttcacc
   113821 gtttcgtaca cggtacatcg ggtttttagg atcaggtacg attgatgtca cgatggcttc
   113881 ttctatgaat ttacttgatt taccataata cacagtacta atagaagaag ttggattttt
   113941 catgccagtt agtgccgcaa accaatcgta accaacactt gctgcagctg gttcatttcc
   114001 ttctgctact tctggtaagt caattgattt tgcttgatgg ttagcaattg cttcattgta
   114061 atcgtcaatg gccattggtt ttgaaagatc taaagaatca agtaatgata aataaaatgc
   114121 tgagctttca ctgcgatgcg tgtcttttcg ttcgtccatt gctgcgatga cagcagcttt
   114181 gattgcttct acactatcag tcactaattc atcaatgatt tttgattcta cccgttgacg
   114241 agatcctaat gtattccaaa tcaagtcttt gtcactagaa tcaaattctc gaacgcctgc
   114301 ttcttgttca ataacttctg gtccattcaa accgactctc gctttttttg ttgcgattaa
   114361 ataactcatt aatgctgaag taatcgacat accgccaaat gatcctacac gtcctggaat
   114421 caaaccgatg actggtacat attgttttag agcaatgatt acatttccga tttctgaaat
   114481 agataacagt ccgtagtttg cttcttgcaa acgaacacca cctgtatcta gtacaatgac
   114541 tggaaagatc tcatttcctt ttttattttc ttctaacaca tgttcaagtg cagcaacgat
   114601 ttttgcaccg gaaacttcac cgataccgcc accttggaat tttccttcca tagcgattac
   114661 tactgcttgt ttgccattga tcgtgccacg gctgacaacg atcccgtcat cactttcagg
   114721 gacgattcct tgtgctggta aacttggcgc gatgatttga tcgaatggat caatcaattc
   114781 acgtccattg tcaagtagag cgttcactcg ttctctggcg tttaattcta caaaactatt
   114841 tttcatctga aagttcctcc attgcttgcg tcaatcgtag gtacacaact cctggtgtag
   114901 caccgaagtc attgatcacg atatctgctg aaattggata acgatcaaaa aatctcgtta
   114961 atacattgtt ccatacttcg ccgaagccat cgcttcctgt acaaattttt acagtagatt
   115021 ctgttttttc tgttggaaac atcaagattt ccaaatctcc tgaaccgacc actcctacat
   115081 ggattttctt attgatcggc tgtttcgttg gatatttata agctaattgt tccatggatc
   115141 tctccccttt ttgtaaaaca gagcatacac gttctgctta ttcgtctttc ttcttccatc
   115201 tgggtaaatg gcgacaaaag gtttttctcg ccaattttat gaccaagttc tgaatttcat
   115261 tggtggttca tacaggccat ctgaccattc gatcaaatca tcgatggttt gtgcagctaa
   115321 taatgaacgt ttcgcttcac tacgtttgat gcctagatct tctggtaatt tcacgatgcc
   115381 ggctgcacgc aagctatcta gtgattcttt cgtgctagtc attccaagag gtgtaacacc
   115441 gccaattgct gcaattgctg cttgtcgttc ttcttttgtt tttgctttgt acaaataagc
   115501 aattccttct tctgtcacga tatgtgtagt atcttcgcta taaatcatca ctggtgcatt
   115561 ggctaagcct gcttgttttt gaacagctag tgcatctaat tcttcgacaa agactggttt
   115621 tttatttgaa ccatatgttt caaccatttg aacaactaat ttttggcctt tccctaatgg
   115681 atcatcattg tcgcgaagtg attgccatgc tggtgtagaa tgacgacgac cgccaggatt
   115741 atgtcccatg ttaggcgcac cgccaaaacc agataaacgt ccttttgtta cggtagaaga
   115801 gtttccttcg taatctaatt gcaaggttga accgataaac atatcgatgg catattgtcc
   115861 tgcagcttgt gataatgttc ggttactgcg catcgtgccg tctttaccga caaaaaagac
   115921 atctgggcga gcagcaatat atttttccat accaacttcg ccaccaaaac tatggatgct
   115981 ttcaacccaa cctgattcga tggctggaat catcgttgga tgtgggttca atacccagtt
   116041 cttcgcgatt ttccctttta accctagtga ttcaccataa gttggcagta acaattcgat
   116101 agctgctgta ttgaatccaa taccgtgatt caaagattgt acgccgtgtt ctgcgtagat
   116161 tcctttgatt gccatcattg ccattagaat ttgcaattct gtgatgtttt gaggatctcg
   116221 tgtgaataaa gcttctaatt gataaggttt atctgctttc acgacaacgt ctacccagtc
   116281 accagggata tctacacgtg gtaaagaatc gacaacttcg ttgacttgaa cgatcacgat
   116341 accatctttg aaagctgcag cttcgatgat tgttggtgtt tcttccgtat tgaaacctgt
   116401 gtacaagttg ccgtctttat ctgctttatc tgcagctact aaaacaacat ttggaatcaa
   116461 atcgatgaat aagcgtccat aaagttccaa ataagtatgg atatcaccta atttcattgt
   116521 gccgtcttca atcatttggg cgacacgcaa gctttgtgca ccagcataag agaaatcgat
   116581 cttgcttgcg attccttttt caaaaatatt caaatgttct ggtcgtgaga tactagacat
   116641 gatcaaatgc agatcattca ctttttctgc atctactttt tcaagacttt ctgaaagaaa
   116701 acttgcttgt ttttgattgt cgccttctaa tacaactttg tcgccgggag caatcagttt
   116761 ttccattact tctacgattt tttcagtttc tacatacttg ccattgagca agttgctgat
   116821 tttttccaat cgctgcgctt ttttttcgcg tttagaagcc cagttgcggt ttggtttttc
   116881 cataattagt catctccatt tttgatatag aaacgtggcg tcatcaatac catgttggtc
   116941 tagattgatc gtacggatcc ctttatctac accaaatttt gtatgaatca gctttgtcaa
   117001 agtattgccg gggggaaatt caatcgtcag atccattcct aattcttttg ccaagtccat
   117061 catttggttc cactgcacag ggaaagctag gttttccaac aaatctttac gaatctcttc
   117121 tgcgtcctga gtggctcttc catccgtatt ttttatatac aaacatctag catcagatag
   117181 atggacagac tgcatatacg gcttgaattt cttgaccgta tcattcatca agagacagtg
   117241 agatggcaca gggactttca gcagcttcgc tgtttgcgct tggtgccgtt tggcttctgc
   117301 caacgtaacg gataaacctt cgatactccc agataacgta tgttgcatcg gacaattttc
   117361 atttgacaga taaactggat ggtcaggatc aaatgttttt gctacaattt cctcagcttc
   117421 gcttctcgtc acacctacta ctacgcccat tgcataaccg cttggataag cttctttcat
   117481 taagactgcg cggtgataga ctagttccgc tgcattctca aatctcaaag atcgacaagc
   117541 aacagctgca caaaaagcac caatcgaatg tcctgctgca atatcaggct gaataccgtt
   117601 tttttccatt tcatctaaat aaaatactgc ttttgtcagt aatgctagct ggataaaaac
   117661 cgtatcttga tagttttctt ctttatcaac taaccgatac cccgtcacat tttctaaacg
   117721 ttccatgtac atttcaggga tatccgctaa catccctaac ttctgactgc cttgtccagg
   117781 aaataagaaa cctgtactca tgagcctcgc ctcctcagca cttacttttt ccactgctat
   117841 cttagtcaat ttctgctata ataaaaaatg ccaaaaaaag aaaaaaccat aacaaaaagg
   117901 tatggatggg atggataatt atgaaattgc ttcatctgca gtattttaaa aaagtattcg
   117961 aaacaaaaaa tgttacccaa gcagccaaag aattatttat ttctcaacct gccttgagcc
   118021 gtgcgatcaa acatttggaa aacgaattag gtgtcccttt gttttaccat agcggcagaa
   118081 acattgagac gaccgtttat gcagaagaat tttatccata tgcagtcaag acattagaca
   118141 cattagaaga aggaatcaaa ttattgtctt cggtcaatga gaagatcgtt acttccgtca
   118201 tattgtattt agaggtagcc tctgtttcta ttccgaactt ggtccgaatt tttatggaaa
   118261 agcatcctga tatccaatta acgatcatgc aacatcattt accagatgat attaccgatc
   118321 ctgtactgta catcacttcg gagatgaagc ctggcatgac caatattcct atcatgaaag
   118381 aacctgttta tgtcgccatt cctaagaaac atcctcttgc taaaaaagaa catctgcaat
   118441 tagaagatat ccaacgcacc cccatcttga tgctgtcaaa gagcaacgcc ttccgtcata
   118501 cactggatgc agcacttgaa gcgaaaagta tcgaattgac gatcagttcg acgacagatg
   118561 atcctgcgac attacgttct attgtcaagc aaggattagc tgttagcttt ttcccaaaag
   118621 tttcttggtc ttacgataaa aatgatcctt tcgtcttgcg ggaaatcaaa gatttgccct
   118681 tgacacgaac aatttattta tcttctagct tcacagaaga tcatcctttg atcaaaatga
   118741 ttgcaaaaac gatgcgggaa ttttatttag gaaaataaaa aaaaaagagc tgggacaaaa
   118801 ttcttgtcgc agctcttttt tcgaacaaac ggtgttccag aagtaatttc ttagaaaata
   118861 agccgaaatt tcccaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa
   118921 gctaaacatt tctgtcacaa cctcttctct attgtcctag acattcattg cgttgattta
   118981 aaacttccat caatcaaatc tgctagttca tctagctttt ctattttacg tagatcaata
   119041 gagatccaat gttctttatt catgtgatag gctggaaaat acccctcttt tcctcgcaaa
   119101 attccgccaa gttcaggatc gaccttcaaa tcaagaatat tgatgatcct atggtcactt
   119161 gcatcatcat agattttttc ttctggaaca gacatcagga ccccaaacca ttttttgctt
   119221 ttttgatgtc tgaatacgca ataatcagga aattttttaa aaatatattc tggagacaaa
   119281 ttgtatgttt cattgatgtg atcgattagt tcttttttcg ttagcatctg cttccatctc
   119341 tttcttattg attcgcttta ttcaaagata ccacgtaaga atagaaaaat gaatacaagt
   119401 agttgctaat caaatacttc gatatttttc ttttttatca actcttaaat gttcaatccg
   119461 cctgtgttgg tcttgctacc atctactatt aaaatgttta ttattgtttg cccacctatt
   119521 ttttgatttt agtggtatca tttttcaagt tcatgatgaa attcttatca tcagtaaatg
   119581 ctccagaaaa ataaacaatg attgaaaaat cagttgaaac ttgaccaccg tttattttct
   119641 ccgtattctt agaatagaag ggagtctgat tgatgtattc actaatgaaa aaaatcatta
   119701 ccgaaaaaga tattcgacgt catgtttctt tagtagaaca attgctgaat catacaaaga
   119761 taaccgtaaa tgaattagct gaaatcatag ggacaaccga acgaacgatt ttttctgacc
   119821 ttcaatctat tcgctctcag ctgcccgaag gctgggacat catcagcgat caagcaggaa
   119881 tcagtttgca aaaccagcaa aatttattaa caaatgacct ttgggagatt tttttcaaac
   119941 aatcagtcag tgtcgaactg ctgaaaaatc tgctttttac aaaaaaagtt gctgtccctg
   120001 attttctagc cgaccatggg ctttcttatg ggacattgaa aagacatgtg actaaaatca
   120061 atcagcgttt agcaagttac gacttgcaga tcgatttgac gaaatataca gcttgtattc
   120121 ttggaaaaga gcgtgcgatt cgtacttttt atcatcgatt gctgattcct ttcactcata
   120181 acaactattt tttcgaggat tattcgatcc atgaatccca ttattttcaa tttttaagaa
   120241 acctcagtca aactgaacta gcggttgaaa cagaagagat tttcggaaca tgttggttct
   120301 ttatcaatac gatccgtata aaagcgaatt gccgattaga ttcttctatc cataccaaca
   120361 gcacactctc ctccctgtat gactctgcat tgaaaaaatt gtatttaacg gaaggtattt
   120421 atcttaaaga cactgagctt tcttttgctt ctttctgctt tctggaaagt tggaattaca
   120481 acaataacta tggacaagaa accgctcgct gcctgcatca ttctccattt ttagaggtcc
   120541 tcgaaacgtt tgtagaggaa ctagcttcag agctgtctct tgatcaacta aaaaaaacat
   120601 ctctgactga taacttggct ctcctcatat taaaatatca tgaatcgccg atattgattg
   120661 aagcattgga ccgtcaatac catcgttttt tagaaacata tgatcagcaa tatgcacact
   120721 tgtatcaaca gaaagagaat ttactgaacg atctaaaaaa agaaatagaa atcaatgaac
   120781 caaattactt tctccaactc ttgtctctac tgatccaaca aacgattttt tctatcaaac
   120841 cgaatttgtt caatgtctat tttattttcc aaggagaacc ttcatggaag gcgttcttgc
   120901 agcaggaatt aaaagattac ttaggaaaac gtgtctgctt cttacctatc gaactaaccg
   120961 atctttccac tatttctttt gaaaaaacag atatcttgat ttccaatttc cctctagatt
   121021 ctctagatat ccctattgta tacatttctt ctattccaac gaaaaatgag ctcaatcgtt
   121081 tgactgaact cactttcaaa acttttttat agtaacggct agcgactgta ttctctagct
   121141 gttttttctt ttttgcgaaa cagtttgttc atgatccctt tttctatttc tacaactaag
   121201 aatagaagaa tcacatttgc tccaatcaat aactgttgtg tcatcgttaa acttactgta
   121261 ccgatcagct gctggccgac tggtagaaaa ataaccatcc cttgcaataa agcaaggata
   121321 cctaatgaaa gaaaaagcgc tttgttttgc aaaagacctt ggttcaatga cggcttggac
   121381 aactctctgc aattgatcat gtaaacagct tgagctaaga cgatattttg cagaagaatg
   121441 gtttgctgta agctttgccc ttcaaactgc atagccaata aataggcagg aaccatgatc
   121501 agtagggaga catagatgat ccgaaacaaa tgataaccag ataggattcc ttgctttgga
   121561 tctcttggtg ggcgcttcat gatttctgga tctggcgact cgaaccctag tgcataagat
   121621 aaagtgattg tcgttaccat attgacccat aaaatctgta ctggggacaa aggtagcggg
   121681 cgattcagca taagtgccca aatcaagatc aatccttgag ctaatgcagt tggcaagaag
   121741 aaattgatgg ttttttgcaa attatccaaa atccgccggc cctcttttac ggcttttgca
   121801 atcgtatgga aattatcatc tcctaaaacc atatcagctg cctgtttgct tacttcacta
   121861 cctttgattc ccatagcaat tcctacatcg gatcttttca aagcaggtgc atcatttacg
   121921 ccgtctccgg tcattccgac gatttctcca tttttctgca aagctttaac aattcgcagt
   121981 ttatgttctg gtgtcgttcg agcaaagaca tcgactattg gcacttgctg aatcaactct
   122041 tcatcagaca taagatccaa atcgatccct tctaaaactt tggcagtatg ttttaagcca
   122101 atttgttccg caatcgcttt tgctgtgtct ttgtgatccc ctgtaatcat tttcactgag
   122161 atccctgctt gttggcattc ctttacagct ttgactgcac tttcttttgg cggatcgatc
   122221 aatccggcga tccctgcaaa agttaattgg gttaaagtct catgcgtgag ttcctgtttc
   122281 gaatctactt ttttataagc aaatcctaaa acacgctgac cttttctcgc aaattctgca
   122341 gctgtctgac tccatgcttc ttgttctgca gaagaaagag ttgatttagc aaataatact
   122401 tctggtgcac ctttgacgta aatcaccgct cctttttcct ccgcatgcat cgttgccata
   122461 tatttgtatt cagaactgaa cggaatcttt gcttctattt ttttcaaagg tagattggct
   122521 gtttctgtgt gtcgaatcag cgcaacttct gtcggattgc cacttaaatt ttctgttttc
   122581 tgttcttctt ttgtttttaa ttcctgacag tttttcataa tggacaacac gcaggcttct
   122641 tcttttgcag ctacatccac aactgtcatt tcattttttg tcaatgttcc tgtcttatct
   122701 gaacagatca ctgtcataga acctaatgtt tctaccgaag gaagtccttt gatgatcgcc
   122761 ttttcttttg ccatttcatg tactccaacg gatagaatca tggtcaaaac agctggcaat
   122821 ccttcgggta ccattgacac caccaaagca atcgtagccg aaagaaggat atgccaatcc
   122881 ataccatatc gaaatgtcgt aaaaataact aagaataggc tcaaacaaag aatgccttga
   122941 aaaatttgtt tgtttaatcg gttgattttt ttaatcagag gcgtcgtttg ttgttcgacc
   123001 gattgcaatg cttgattgat tttaccgatt tctgtcgtgt ttcctgtttc tacaacgaca
   123061 ccgactgcag aaccagattg tacaagcgtt cctgaaaaag ccatattttt ctggtcgccg
   123121 atcgattctt cttctgtcaa aacggcatga cttttttcag atgcttctga ttctcccgtc
   123181 aaaatcgatt cttccactaa taaatcatgt gcttcgatta atcggatatc tgctggaacg
   123241 acatctccgg cacgtaagac aatgacatct ccttttacca gttctttcgc agggattttt
   123301 tcttgcatcc cgtttgatag tactatcgct tcttgtccca tcagctgctt caaaccattc
   123361 aatgattctt ccgcttttct ttcttgccag taactaacga gtccattgac gatcactacg
   123421 aataaaataa tacttccttc aatatattca ctagaagcaa atttcaaaaa agaggcaatg
   123481 atcaacacga tcatcagcag atcagtaaaa tgctttgcta tttttttcca tcgttttgtc
   123541 tgctgccttg cttttatttc attatatcct tgatcagtca ggcgtttctg tcgttcttct
   123601 tgcgacaagc cttctgttga tgtttgatag tttttcaata catcttctac cgtcaatctg
   123661 taccagttca tcctatcccc tcctgtttaa ttgatactgt tattatacgc agggaagttt
   123721 tttcaggcgt atcgattatt tcaggggggt ctgaaataat tcgttttgca aaatgtatct
   123781 gggcctaact gatatttcta ttgcttattc ttttatatac aaaatttaag aatacaaacc
   123841 agataaccaa taaaaaagga ataaagcgtg tcgtgctttc gaataacaag ctgaaagcaa
   123901 ttgaagtaaa aaacaccaac aaagcaagat ctttatatct ttgtgtagtg ttccgtttct
   123961 tttctttaat aaattgaaca tgtgcacaca agatcgaaga ccacacaaat agaaaacaac
   124021 tcgttgtcat gcctgccaaa agctgaaaaa acaatcgact atcagctaca aaaaaatgta
   124081 gacaaagacc ggcaaaaaat aacattcctg taattaaaat accgtgtaaa ggtacgcctt
   124141 tcttagaaag cgcacaaaac caatggggca aacttttttc ttttgctgtt tgatataaaa
   124201 tcctgctagt actgtagact gcactatttg ctgaggacaa agaagcaacc aaaatcatta
   124261 gattgacaat tgctgctccg ccttggcaat ggatcacttg cagcatagtc actaaaggac
   124321 tttcttttaa atctaaacaa tgccatggaa caacaaataa aatcgacaaa atcgttccta
   124381 tatagaaaaa gagaatacgc caaggcagac agttgattgc ttttttcaaa ttcttttgcg
   124441 gatcacttgt ctcgccaacg gttaagccga tcatttcaat tccagcaaaa ctgaataaaa
   124501 ccataggaaa tgagactacc attccagcaa agccaaacgg tagtaattca tcgatctgtc
   124561 caaaattttc tttgattccg atagactggg tatgaaaatc tgagacactc aatcctatac
   124621 ccaataagat aaaaaggaaa atccccaaga tcttgatgat tgagaaaaaa gattccaatt
   124681 caccaaacca cttgactgca acaaggttga ctgccattaa gatcattagt gcgctacctc
   124741 cgcttatcaa agctggtaca tgtacaaacc agaaatggat atacaatcct attgctgtta
   124801 attccgcaag tccagctcca agccagcaaa gccaataagt ccaattgaca aaaaatgccc
   124861 actttttgcc taaataatag tcaacgaaat cactgataga gttaaatgta ctatttgcta
   124921 aaaataaatc acccatggcc cgcatcaaac cataaatagc caaaccgcat atcaaataaa
   124981 cgagaataac agctggccct gctgatgcaa ttgcctttgc tgaacctaaa aataatcctg
   125041 ttccaattgc gccacctaat gcgattaact ggatatttcg attagttaga gtccgtttca
   125101 ttgccggttt ttccatcaag ttcacctttt tctttttcaa gcagcatggc gacttcctgt
   125161 tcccaagaag gtttctttat tttttcaata cagagtggta ctacaaacaa aatgacactg
   125221 atggctgtga ctgcaattag attgatccat gttccagctt tcatcgtcag tccgacagaa
   125281 aatccgattg ccgcaaataa aacaataacg acagtccaaa ctccaagatt gctctttttc
   125341 tctttttttc caattctaaa tggtcgattc aactttgcat gtgtataacg caatctcaaa
   125401 actgcgatcc ccatgatgac atagacgata cagtagataa cagtagttga atttacgagc
   125461 atcaaaaatg cttgattgac gccaggaatc agcaaataaa aaagtgcaaa gattgagata
   125521 atgacagact gagtcaaaat aacggatttg gatacgtcaa attgattcgt cttccaaaaa
   125581 ttgaattttg gtggatacgc accttttcta gcactttctg tcactgtctt tgctggtcct
   125641 gaggcccaag cagataattg gactgctaca ccaatgaaga caagtaaact aaacagattc
   125701 acaataagat gtggcaaacc aagaatttgg caataaatcg aaatagattg cgcgatatta
   125761 ttcaattcca tttgtccttt tggtacgaca tttgctacaa ctaaagcatt caaaatattg
   125821 acgaaaaata taaaaattaa cgcggcaaat actccgcgaa tatacgtttt aactggattt
   125881 tccaatcgag tgatatacac agaggacatt tcgataccag taaaaataaa catgatcggc
   125941 gcaaaataaa caaatgagga tgcagtcgta gtatctggta ttaatttgtc ccatgaaaaa
   126001 ctgcctaatg ttccggttgg tacgatccca actttgatcc acgcagcaaa ccctaataag
   126061 agcatcatcg tcaaaggcac gtacaatcct aaccaaacac cgacttttcc gccgattttt
   126121 gccatatcaa atttcaaatt caaaagagtg atgatccaat aaacgactaa aatgacaact
   126181 aatgtaaatt tgctgttcaa accaagcggc acattatcga taatattacc aaacaacgga
   126241 gcgagtgctg aagcgaccat caccatccca ggaaacattt ggacccataa cagccatgag
   126301 acaacgaacc cccatttttt tcctagagca ttcgataccc aaagttctgg accacctttt
   126361 tggggaaaca ttccagcaaa ttcaccagag atcaatgaga ttggaaaagc gtacaataat
   126421 actgcaacca gcatgtaaaa aatcatcgtc cagctcgttg ctgctacatc tggaatattt
   126481 cgtacacttg ctacaagggc gcaggtcatt ccgataaagg tcatggtcgt gatttgcttt
   126541 gatgaaccag tatctttcat ctttattcct tctttcaata gcgacatatg tttttgtttt
   126601 cttttatttt tgttcaagaa cagaaatgac aaggtttcat cgattcagta caaaacccag
   126661 tatcaccgaa acatccattg aacgatctta gtgaactggg tttgatacat gaaacgaacg
   126721 atccaactta tagaacgttt tataaaaaag aatatcttgg cgaggaatat atgaaaaaca
   126781 gggggaaata caattgtttt gatgaagcaa ataattgtct gttctccata tgtctcttgt
   126841 tattctttca cacaaaaatc agttctattg gatgatttcg tttagtttct tttccattgc
   126901 tttggcgatt ttttttgtat aaaaattgaa aattcgatca tcattcaaat aaggagtcat
   126961 gatagccgca cgtagcagag tgattttctg ttcttttttc cattcttctt tcgttagacc
   127021 cattctttca acaaaacgaa tcggagaatc accgtaatct tcttgtgtaa atgtagtatg
   127081 agaagtaata aaccgttcac cataaacatc gccatccata taagaagacc gatcgaacat
   127141 tttttcattt aattcattga tagcgttcaa atctgtacag ccttgttctt tgaataccca
   127201 attgaccata ttaaaatcag gatgattcaa tggatagact tcgattgttt ttcctttaac
   127261 agtaaaagtc agatgatcga gaaattctct gaatctttga gctgcctcga ttgaagcccc
   127321 aatcaattga ccatatccgg tcacattcaa tggcaataca cgatgtgctg tccagactgc
   127381 ggcggcggtt gctccagctt ttgatccttc caaaatataa gctcctaaca tatctggagc
   127441 ctttactgat ttttcaaaca catatggcgc gaaataagaa atgatatttc tcatattttt
   127501 atgtttgatt acgattcctc cagcagcata agggacatag cccatcttat gaggatctat
   127561 cgtgacagaa tccgcttctg aaatagctct gaacccttca tatacctctt tgtcaatcgt
   127621 tacgcagtgg tggaaaacat gatgttcttc aaagaattca gctaaggaag catacggcac
   127681 aaattctcct gcttcgttta gaaacagtga acgcgcatag ccgccatatg cggcatcaac
   127741 atgtaaatag aaatagatcc cttcatcttt taacctttct cttaattgga cgattttatc
   127801 tacactgtct acttgacctt cttctgtcgt tcctacgaca gcaaccacac ctagaattgg
   127861 gattttttgt ccagctaatt cacgaattgt tttttccaaa gcattaatat ccattcgata
   127921 atcctcttga actgggatag caaccatctg atctaagccg actccgcaaa tatctaacgc
   127981 cttcatccaa gaatagtgtt ttgtttgagg gaccagccat ttccctaatc tttggatatt
   128041 ttttccgcta cgggaagaag ctgctttgac ctcatccatt tcttcgtccg taaagctttc
   128101 ggtcatttcc aaaatttctt caacagataa attcaaaagt tcccactcag acattttttt
   128161 cactttttca ggcagcactt ccttgacagc taaaggaatc gatttgatgc aacgggcgta
   128221 ccataatcct tcaagattag caatagaacc atctgctgca atgtgtcccc atccgtctgt
   128281 catattgaat aatgaagcaa aatcctgtcc tacttctgct tccatttgcg aagttgccat
   128341 agaagattcc aatgcaacat tatttgggtt ccacagcatc gcatagttgt aagctaataa
   128401 agcaggcatc aatgtttcag cattcatttg tccccagtaa cggcctgctg aatgccatgg
   128461 gatcgatcct gcgcgtaatc gctgactgac ttcatccaag acctctatca tatggtcgcg
   128521 ggtggctaga taatctggag tgagcttatc tccttcgcta attgcaggca tatctgaagg
   128581 aatataattt tctctccatc caagatgttc atctaccatc ttgttcaaca acattttata
   128641 aactggtcca ttttcagctt tgtcgcctat aaagacggcc ttgatatcca tatctttcat
   128701 ttttcttccc tccatatttt gttggtcaat gtcttgcaga tacatgtgtt tatctgtaac
   128761 acctcttcac tatagcgcca aaaatgagaa attttttttt ataacatcaa atccacgtag
   128821 atttttacaa acaaaaataa ataaagtcgg atttttctga taattcaacg caaaaaagcg
   128881 aacaattttt caataaatca gtcttctaaa atagataacc taaaaaaag
//
LOCUS       DARBDV010000002        74717 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000002 DARBDV010000000
VERSION     DARBDV010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 74717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.002"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(105..629)
                     /locus_tag="SCM56_000110"
     CDS             complement(105..629)
                     /locus_tag="SCM56_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1108944.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="SCM56_000111"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="SCM56_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEG1108945.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="SCM56_000112"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="SCM56_000112"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEG1108946.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="SCM56_000113"
     CDS             complement(2351..3031)
                     /locus_tag="SCM56_000113"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEG1108947.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="SCM56_000114"
     CDS             3157..4026
                     /locus_tag="SCM56_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG1108948.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="SCM56_000115"
     CDS             4052..4567
                     /locus_tag="SCM56_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108949.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5207)
                     /locus_tag="SCM56_000116"
     CDS             complement(4614..5207)
                     /locus_tag="SCM56_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1108950.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="SCM56_000117"
     CDS             complement(5232..5411)
                     /locus_tag="SCM56_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108951.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="SCM56_000118"
     CDS             complement(5488..5997)
                     /locus_tag="SCM56_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108952.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="SCM56_000119"
     CDS             complement(6093..6473)
                     /locus_tag="SCM56_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG1108953.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /locus_tag="SCM56_000120"
     CDS             6803..7552
                     /locus_tag="SCM56_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HEG1108954.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7736..9013)
                     /locus_tag="SCM56_000121"
     CDS             complement(7736..9013)
                     /locus_tag="SCM56_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108955.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="SCM56_000122"
     CDS             complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="SCM56_000122"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEG1108956.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9935..10879)
                     /locus_tag="SCM56_000123"
     CDS             complement(9935..10879)
                     /locus_tag="SCM56_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEG1108957.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10881..11567)
                     /locus_tag="SCM56_000124"
     CDS             complement(10881..11567)
                     /locus_tag="SCM56_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEG1108958.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            12041..12361
                     /locus_tag="SCM56_000125"
     CDS             12041..12361
                     /locus_tag="SCM56_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEG1108959.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            12515..13420
                     /locus_tag="SCM56_000126"
     CDS             12515..13420
                     /locus_tag="SCM56_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1108960.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            13417..14190
                     /locus_tag="SCM56_000127"
     CDS             13417..14190
                     /locus_tag="SCM56_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEG1108961.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(14371..15795)
                     /locus_tag="SCM56_000128"
     CDS             complement(14371..15795)
                     /locus_tag="SCM56_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1108962.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            16006..16452
                     /gene="nrdI"
                     /locus_tag="SCM56_000129"
     CDS             16006..16452
                     /gene="nrdI"
                     /locus_tag="SCM56_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEG1108963.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(16517..18499)
                     /locus_tag="SCM56_000130"
     CDS             complement(16517..18499)
                     /locus_tag="SCM56_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1108964.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(18513..19409)
                     /gene="murQ"
                     /locus_tag="SCM56_000131"
     CDS             complement(18513..19409)
                     /gene="murQ"
                     /locus_tag="SCM56_000131"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEG1108965.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(19412..20473)
                     /locus_tag="SCM56_000132"
     CDS             complement(19412..20473)
                     /locus_tag="SCM56_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEG1108966.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(20684..21250)
                     /locus_tag="SCM56_000133"
     CDS             complement(20684..21250)
                     /locus_tag="SCM56_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEG1108967.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(21278..22780)
                     /gene="eat(A)"
                     /locus_tag="SCM56_000134"
     CDS             complement(21278..22780)
                     /gene="eat(A)"
                     /locus_tag="SCM56_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HEG1108968.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(23038..24159)
                     /locus_tag="SCM56_000135"
     CDS             complement(23038..24159)
                     /locus_tag="SCM56_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEG1108969.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            24285..25226
                     /locus_tag="SCM56_000136"
     CDS             24285..25226
                     /locus_tag="SCM56_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEG1108970.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(25376..26272)
                     /locus_tag="SCM56_000137"
     CDS             complement(25376..26272)
                     /locus_tag="SCM56_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HEG1108971.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            26541..27881
                     /locus_tag="SCM56_000138"
     CDS             26541..27881
                     /locus_tag="SCM56_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1108972.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            28099..28542
                     /locus_tag="SCM56_000139"
     CDS             28099..28542
                     /locus_tag="SCM56_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEG1108973.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(29046..30464)
                     /gene="nhaC"
                     /locus_tag="SCM56_000140"
     CDS             complement(29046..30464)
                     /gene="nhaC"
                     /locus_tag="SCM56_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEG1108974.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(30867..32090)
                     /locus_tag="SCM56_000141"
     CDS             complement(30867..32090)
                     /locus_tag="SCM56_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEG1108975.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(32121..32960)
                     /locus_tag="SCM56_000142"
     CDS             complement(32121..32960)
                     /locus_tag="SCM56_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEG1108976.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(32977..33657)
                     /locus_tag="SCM56_000143"
     CDS             complement(32977..33657)
                     /locus_tag="SCM56_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1108977.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(33659..34732)
                     /locus_tag="SCM56_000144"
     CDS             complement(33659..34732)
                     /locus_tag="SCM56_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1108978.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(34788..35026)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(35069..35302)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(35379..36050)
                     /locus_tag="SCM56_000145"
     CDS             complement(35379..36050)
                     /locus_tag="SCM56_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEG1108979.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(36137..38815)
                     /gene="mgtA"
                     /locus_tag="SCM56_000146"
     CDS             complement(36137..38815)
                     /gene="mgtA"
                     /locus_tag="SCM56_000146"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HEG1108980.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            39474..39911
                     /gene="ndk"
                     /locus_tag="SCM56_000147"
     CDS             39474..39911
                     /gene="ndk"
                     /locus_tag="SCM56_000147"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEG1108981.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(39933..40799)
                     /locus_tag="SCM56_000148"
     CDS             complement(39933..40799)
                     /locus_tag="SCM56_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEG1108982.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(40959..43052)
                     /locus_tag="SCM56_000149"
     CDS             complement(40959..43052)
                     /locus_tag="SCM56_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEG1108983.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(43801..44424)
                     /locus_tag="SCM56_000150"
     CDS             complement(43801..44424)
                     /locus_tag="SCM56_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108984.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(44583..45980)
                     /locus_tag="SCM56_000151"
     CDS             complement(44583..45980)
                     /locus_tag="SCM56_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG1108985.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(46012..46983)
                     /locus_tag="SCM56_000152"
     CDS             complement(46012..46983)
                     /locus_tag="SCM56_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1108986.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            47357..47782
                     /locus_tag="SCM56_000153"
     CDS             47357..47782
                     /locus_tag="SCM56_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108987.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(48270..48971)
                     /locus_tag="SCM56_000154"
     CDS             complement(48270..48971)
                     /locus_tag="SCM56_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEG1108988.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(48987..49427)
                     /locus_tag="SCM56_000155"
     CDS             complement(48987..49427)
                     /locus_tag="SCM56_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HEG1108989.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(49427..50002)
                     /locus_tag="SCM56_000156"
     CDS             complement(49427..50002)
                     /locus_tag="SCM56_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEG1108990.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(50850..51329)
                     /locus_tag="SCM56_000157"
                     /pseudo
     CDS             complement(50850..51329)
                     /locus_tag="SCM56_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(51488..52189)
                     /locus_tag="SCM56_000158"
     CDS             complement(51488..52189)
                     /locus_tag="SCM56_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1108991.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(52297..53706)
                     /locus_tag="SCM56_000159"
     CDS             complement(52297..53706)
                     /locus_tag="SCM56_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEG1108992.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(53754..55619)
                     /locus_tag="SCM56_000160"
     CDS             complement(53754..55619)
                     /locus_tag="SCM56_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1108993.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(55740..56591)
                     /locus_tag="SCM56_000161"
     CDS             complement(55740..56591)
                     /locus_tag="SCM56_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG1108994.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(56901..57614)
                     /locus_tag="SCM56_000162"
     CDS             complement(56901..57614)
                     /locus_tag="SCM56_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HEG1108995.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(57682..58008)
                     /locus_tag="SCM56_000163"
     CDS             complement(57682..58008)
                     /locus_tag="SCM56_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEG1108996.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(58131..58358)
                     /locus_tag="SCM56_000164"
     CDS             complement(58131..58358)
                     /locus_tag="SCM56_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEG1108997.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(58680..59933)
                     /locus_tag="SCM56_000165"
     CDS             complement(58680..59933)
                     /locus_tag="SCM56_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEG1108998.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            59994..60734
                     /locus_tag="SCM56_000166"
     CDS             59994..60734
                     /locus_tag="SCM56_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1108999.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(60820..61578)
                     /locus_tag="SCM56_000167"
     CDS             complement(60820..61578)
                     /locus_tag="SCM56_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEG1109000.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(61575..62603)
                     /locus_tag="SCM56_000168"
                     /pseudo
     CDS             complement(61575..62603)
                     /locus_tag="SCM56_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(62623..65790)
                     /locus_tag="SCM56_000169"
     CDS             complement(62623..65790)
                     /locus_tag="SCM56_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109001.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(66014..66898)
                     /locus_tag="SCM56_000170"
     CDS             complement(66014..66898)
                     /locus_tag="SCM56_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEG1109002.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(66993..67430)
                     /locus_tag="SCM56_000171"
     CDS             complement(66993..67430)
                     /locus_tag="SCM56_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109003.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(67647..68558)
                     /locus_tag="SCM56_000172"
     CDS             complement(67647..68558)
                     /locus_tag="SCM56_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEG1109004.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(69015..69443)
                     /locus_tag="SCM56_000173"
     CDS             complement(69015..69443)
                     /locus_tag="SCM56_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109005.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(69471..70109)
                     /locus_tag="SCM56_000174"
     CDS             complement(69471..70109)
                     /locus_tag="SCM56_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1109006.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(70113..72095)
                     /locus_tag="SCM56_000175"
     CDS             complement(70113..72095)
                     /locus_tag="SCM56_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HEG1109007.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            72275..72940
                     /locus_tag="SCM56_000176"
     CDS             72275..72940
                     /locus_tag="SCM56_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1109008.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
BASE COUNT        21713 a        15532 c        12741 g        24731 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct attttttata
     7741 gtaattcctt cagatacttt cttgcctcat cattgaaacg cagtgattct tctaacttct
     7801 gataatattc caacgcttgt ttctgagctt cagtaaaacg ataatgtttt cctttccatt
     7861 tgtcgattgt ctcttgcaca tttccagatt ggatatcttt cactactagt tgccattttt
     7921 cttttgagac tgtacggtct ttcttatcag cagattgtat agcttctcgt tctttacgca
     7981 atacttttaa aaaaccattt ctgactatcc gaggaatgct gtcgaagaac ccttctgtaa
     8041 aaccatagcg acaatattgc ttccaaataa atagatagat ttttggttgg atgaatcgtt
     8101 ggtttttcgc aaaatattcc atctgagcag tgacttctgg tgttcgttga cgttttgtct
     8161 ttctcaacac ggaaataaat gtttcaaaat aagcattagg atgtactggc acttcttcat
     8221 atacgatgga ttgccaatct ttacgtaaaa atgcgcgata gttgatcaag aattcttcta
     8281 catcctttac taattcttct gcaaagtcgt caaatagttc tttttttatc tgtcgctttt
     8341 ttgactgccc attgatgtgt tcacttattt tcttgatttc attttccaat gcttgataat
     8401 ccgctatgta aatcggcatt tctacttggc gaaattctgc gagccgctga gctaatttta
     8461 tatccggtaa atatggctgt ttcatccgac gattttggta caagacaaaa cagtattttt
     8521 gccatagtcc ttcaggaaaa tcgatattct tctgcttaag ccataataat tcgtctaagg
     8581 ccagatcaac ttttgtcatt ccctgatgga tcgaagctgc cactgtcgga gagacatgca
     8641 ggtgatcggc aaagtggttg atgcctagtt ttaggatctt tccggttttt ggcgatttaa
     8701 cgatgtattg cacttttaat tctctaccac actcacaata caatttttgc tggcttcgtt
     8761 ttgttcgaaa aaacggattg atcttgaagt cagaaaacac ccaatctgtt gcagctaaat
     8821 aatgttcttc cataaaacga gagctgatta gatatcggcg gtgcttatcg actagttccc
     8881 gctgtttttt cgttaatttt gcatagatct cccaacgttg actgcttgtg tattgtttag
     8941 aaattttgat gggatagctt ttgctgattt cttttggaaa ccattcttca aagacggatt
     9001 gccattcttc cacggctgtt tctttccttt ctaattacaa aaagctggct actaacataa
     9061 cgttagcaac cagccagtgt ttcttgctaa attagtgagt tgttccggca tttccggaaa
     9121 tatcttcttt attttctact gctgcttcaa ctgcataagt caaagaagaa gcaatgtctt
     9181 ctaaagacat agaaggcgta cccactggac gatcgatcac ttgatcgggt gagaatggca
     9241 cgtggatgaa cccacctcga attgccggga attgtgtttc gatcaaatga agcaaacgat
     9301 acatgatatc attgcagaca aatgttcctg ctgtataaga gatgtacgct ggtagaccat
     9361 gatcttgtac attttttgtc attgctttga ttggcaatga ggtaaagtaa gcggctggac
     9421 cgttttcttc taaagccgta tcgaatggct gatttccttc attatcaggg attcttgctt
     9481 ctgctagatt gattgcgaca cgttcgaacg agactgctga ccgcccacct gcttgtccga
     9541 cacagatcac gacatctggt tggtgctgtt ggatggcttc tttgactact tgactgcttt
     9601 tttcgaatac ggtcggtact tccactttga tgatttcagc accgctgatt tcatctggca
     9661 ttttttttac tgcttcatag gctggattca ctttgtcccc accaaatgga tcaaaacctg
     9721 ttactagtac tttcatctgt tgttcctccg tccgattgat ttaacatatg gttatctttt
     9781 atcataccat tttccttgaa cagcgtaaat aaaggaaaca atagtttaaa taacataaca
     9841 gatcaaactt aatatattct tataaaaaaa tagtgttcca ctacaatgtt agaagaggtt
     9901 gcgacaatta ttctgtcaca acccctctct tctattattt aaatagaatc atataagcaa
     9961 tttggaaaac tagcataaat aacgcaataa acaactggtt tttgatcact ccgtatttgt
    10021 ctttcatctc cagcatcgct acaggcacga tattgaagtt agcagccatc ggtgtcagca
    10081 gggtcccaca aaaaccgcaa gtcaatgcaa ccattcctat taacgctgga ttcgctccat
    10141 gactaaatac gaatggtgca ccgatcccaa ccgtcatcac ggtgatggcc gcaaatgcat
    10201 tccccatgat taccgtaaat atcaccatgc caagagcgta aatgatgatg ccgatgttca
    10261 catttccttc tggaacgatc gtacctactg cattggaaat cactgtccca actccagcgc
    10321 ttgtaaatac cgcacctaga gaagctaaaa gcattggcaa catgcttaac ggccctactg
    10381 tgcccagcat atctgctgca tcatctaaaa atgtacttgg tcgattatct cgagaaaaga
    10441 acatcaagat tagcatggct gcaaaaacac cgacgcccac gccgactaaa gcacctaaat
    10501 tggtaaatag agcaaacaag atcgcaaaga caccgatact caaagctgga ataaaaattt
    10561 tcattcctag tttgtctgac attttttcca tatattcttt tgatggaagc cggctttctc
    10621 ctttgctgac tttcttagca attgctggta tggtcatcgc aaagatcaag ccaccgttca
    10681 cgattgccgg aagccatctt cctcctgcaa taactacccc taagaccgtc cagaaaaagg
    10741 ctgacccata acgatgagga ttggttttat ctgttaaatt tcttactcca gtgtaaatca
    10801 atacgagtcc catcacgata taaaggatct ccagtaattt atcgcctaat aaaatatcag
    10861 gatttgtaaa aaagtccatc ttattgcccc tttcctggat aatatttctt cgctaatttc
    10921 ttttctttaa gatagaagta gacacttgcc acaactaaag ccgtgatagc cactggaact
    10981 tccactaaag caagctgtcc caatgttacg tcatatccta gatctttcag tgttgattgg
    11041 actagcaacg cccctgctcc tccgatgaat agtacttggc caaagaacca gcagacattt
    11101 tccatagctg aagccatacc ttttagttct tcttcatgct taggatggac ttctaaccct
    11161 tttgattcga tggtccccaa tgccatcggt aaaatgattg gacgaacaaa tccagcaact
    11221 ccaccaaaac tcacgttaaa agccgcaaag atcattcgaa agatcccata aatatctaca
    11281 atcataccag cagatacttt cttgaatcgc ttgatcaagg aagccgctga ttctttcaga
    11341 ccatttcttt caagcgttcc tgttgccaac ataatgatga taaaaatcgc catcccgcga
    11401 ttgtttacaa aacttgtacc taaaagttct aacattcctt gaatgccaag accgccagtc
    11461 aaagctgtaa caactaaagc aaccattacg atcaatatcg agtctaattt aaatgcaaac
    11521 ccgagaataa caatcaagac gcctaaaagg ctaagataat tttccatata ttttcctcca
    11581 taaaatgaca atattttttt aattataaag caaaacacat aaaaaatata gttgttttct
    11641 cacgaaatag acactatttt taaatatttt tcgttatttt tttatcacaa aaattatctt
    11701 acttattgta aggtaaagct ttttttaatt tattgcccga taatatatag aaataaggtc
    11761 tagttactgt tttttttaaa tgattaataa ataacaactt ctttctatga aattgcaaag
    11821 cattcatctt cttttccgct aacgatatat ccgtcagtgc ttggtacact ttactaaaaa
    11881 gtatgagatt gccgatatct ggataggtgt gctatactac agaaaaagat atacaatctg
    11941 ccaccgggta ggataaagag gatgatcatt ccattaggtc tttgaaggga tgattttcct
    12001 ctttttgtgt atttttttat agttaaggag gaatgtaagt atggcatgga tggaattaat
    12061 catagcagga attttggaag tattctggtc aacgatgatg aaatggagtg atggtttttc
    12121 taaaatcaat tattcttttt acacagtgat tggcatgatt gctagttttt actttttgtc
    12181 aaaggcaatc aaaagtttgc cgatgagtct ctcttatcct atctggacag ggattggcgc
    12241 agttggttcg gtcataattg gtgtggtctt gtttcatgat aagctgaatc tttccacttg
    12301 gttctttgtg gggatgctac tcatcagtat cattggcatc aagattacta gcggccacta
    12361 gacacttgga ataagtactg atcagaaaaa ataagccttt ttatttaata ttcaattttt
    12421 ttatgaaaaa ataagaaata agtccaatga cctagcgact tctccactca tcaggtgtat
    12481 gcttgtacta tctatagaaa aggagtgaat atttttgaaa agtctttcgg tccaagaatt
    12541 atccaagaac tttggtcaca aacaagccct cagcgatgtc agttttagct tatcccctgg
    12601 caaaatcgtt ggattagtag gtcctaatgg cgctggaaaa acaacgatca tgaaagctat
    12661 tttaggactg atccattact cagatggaac aatctgtatt ggtgaaaatg agatttctcc
    12721 ctctagtcat aaagggttgg aacaagtagg agcattgatc gaatacccgg gtatctatcc
    12781 attcttaaca ggctatgatc atctgaagtt attctctgaa accaacgatg tatctgctat
    12841 cgacactatc gtgaatcagc tcaagatgga aaagtacatt cacaaaaaag cgaaatccta
    12901 ctcgttggga atgaaacaaa aattgggaat tgctcttgcc ttactgaacc atccgtctat
    12961 cgttatttta gatgagccga tgaacggttt ggatccacaa gcaacaagag atgtgcgaaa
    13021 aatcattacc gacttggcta ctcagggcac ctctttcttg atctccagtc acttactcag
    13081 cgagcttgaa aaaatggtcg atgagctgat cctgatcaat caaggaaaga tcatcaaaca
    13141 atgtacaatg gctgaattga accaaacagc gcaagatttc atcgttatct ctacaacaaa
    13201 tgatctagct gctcgaacga tcttgacaga agctggatat actttggaag atcctgataa
    13261 aatcaagctt ttaaaagaaa acgaagaatt attaggaacc gtgatccagc tattgtctgc
    13321 acaaaatatc catgtgacag acgtccagca tctgacgaat gatttggaat cttctctatt
    13381 ggaaattctg gattcagtaa agggggaaga agaaaaatga cattgcttgt aaaacaagaa
    13441 ttatttaaat tgatcaagaa aaagtcaact gctgttttat ctgttctatt agttgttttg
    13501 ctcataggaa cagcacttct agccaaaaaa tacacgacga tcattgatcc agttgaaatg
    13561 actgctcagc tttttagtgc cacatcttgg attgtcttta tcatgattgc agctgcaagc
    13621 acgatcattt cgatggaagc acaatatggt acattgaaaa atctccttta tcgaaagtat
    13681 tctcgaggag aaatattagt aagcaaatgg atcacattag ttatttattc tgtttattta
    13741 taccttttag ctattattgt cactgtgttg atgaaactga ttcttttccc ttcgatttcc
    13801 ttcacggaga aggtttctac tggtcaaaca ctgatccaaa gcttgttcac ttatactttg
    13861 ggtagttata tcggtttatg gctgatttta agtttagtgt tgatgattgc ttgctttatc
    13921 aatagttccg gcgcatccat ctcagcagga atcgtctttt attttgcttc gtcaatcata
    13981 tctggtatcc ttattgcact catccaaaaa tgggagtgga tcaaatggaa tccgatcagt
    14041 atgttgaatc tgcaaaacca aattggaaac gaagagatca tgaagcaatt gactcatctt
    14101 tctactaatc aaatgctatt cggtaattta gcttacatcg ttttgttctt agcattagga
    14161 tatctcgtat ttaagcgaaa aaatgtatga tgactcatca aaaaagaggc tgtgaaagag
    14221 acgttcagcc ttattgccga ttggctgtct cttaaacacc gtttattctg aaaaaggagc
    14281 tgtgacaaca tttgtgtcac agctcctttc ctagtatgtt tttgaataaa cgacaggctc
    14341 acaacctctc tttatacttc ttttatactt ctatacgtga agcatcccag tggcaaataa
    14401 aaccattgca atcactccga ccatcgtcac acctataagt aaaatcttat ctaagtggat
    14461 atgttgtaca ggctcgtttt gctccttacg tgctttgagg taaaaataaa tgcctggcaa
    14521 gaaaagaaca agaacaagca ttacttcatt aaaggctacg actagcatag cccatacttg
    14581 aaaggcactt gctaaaagcc ctactaaaaa ttggacccat tctttccgct gataagaaag
    14641 tttcatttgg tacaatccta agaaaagcca cgtgaagaaa atagctgctg taccaagtgt
    14701 gtaagcaaag ttataagcac tgtcagtgaa taataatgaa agtaaaaaga cattcgtaca
    14761 taacgtcgtc aatacgatag cataagttgg tgccccattt ttgttgagtt ttccccatgc
    14821 tttaggcaaa agtccgtctt cagccatgtt tcccattgtt tcaatcggta agatcgtcca
    14881 tgagagccaa acaccaacta aagaaatgat caaaccgccg ttgatcacga ctgctcccca
    14941 tttaccaacc atcatttcaa acacatagcc catagcaggc tgcgccttga tcgtttgcag
    15001 ctgattcatc gtcattgtcc catatggaag catggaaacc aagacgtaaa taataatcaa
    15061 gccgaatatc cctagaatcg ttgctttccc aacatctgat tgtttttttg ctcgtgaacc
    15121 tacgacagag gctccttcaa taccaacaaa tacccaaagc atcaccatga tgcaactcaa
    15181 tacttgactg gaaaacggga tcggctgtcc attcgcattc aatataatgt cgctagaaaa
    15241 gttatcaatg aattgttgga tcgaaaacgt gctgatagct actacaataa ataaaaataa
    15301 tggcactaat ttacagaatg tgacgatagc attgacgaaa ctagcatttt cgactccccg
    15361 attcactagc catgcatatc cccataaaaa gactgtactc atcacgatcg atgccagatt
    15421 ttgcccacct tcaaatactg ggaagaaata tcctaatgcg ctcatcaaca atgttgcaaa
    15481 agctacgttg cctaaccagc atgtgagcca gtaaccccaa ccgctgataa aaccagcgaa
    15541 acggccaaaa ccttcttctg cgtaactaaa gatcccgcct tttaaatctg gacgtttttt
    15601 attgatatta ttgatcgaca aggagagcat caacattgct gttcctacga gtacccaact
    15661 gaaaatagct gggccgccag ccgatccgga gatgtccgtc ataataccaa atacgccacc
    15721 tccaatcgct gaaccaatta ctaaggcagc aagtggaatt aaaccaattc cttttttctg
    15781 ttgctctgta tccatgtaag catcctccaa aataaaaccg cttttatcaa aaagtataac
    15841 aaaccttttt tatttttact taattaataa aaactttaat agaaatataa ttctaccggc
    15901 ttttttctca ttatacaagc aaagcgccta gtccctttcc aaattacacc accaaaaaac
    15961 ttaaaaaaga tgctatacta aaaggaaaga attggaggat gatccatgaa cattttatat
    16021 atatcgattt caggaaatac gcgtgccttt gccaaacatc tagcagaata tgctgaaaaa
    16081 atgcatgcag aagatcctgt caatcctgaa gttactttaa aagaaatcca tgaaaatagt
    16141 gattttacga aagaaacaga accatttttt actttcgttc ctacttattt agacggcgga
    16201 aatggtttag ataacggtga tacagaaatc ttgaccgaaa cgatgcgtga atatctggaa
    16261 catgaagaca atcataaact ttgtcttggt gtagtcggta gcggaaataa aaacttcaat
    16321 aatcagtatt gtctaacagc caaacaatat gcacaaacat ttggctttcc tttcttggca
    16381 gattatgaat tacggggaac accatctgat gtgacacgaa tttatcagat tttagcagaa
    16441 aacaataaat aataaaaaag aagaagctga aaaatagatt tcagtttctt ctttcgtttc
    16501 tattgtttct atcatattaa gctttgacat ccatcacagg tgcttttgct tttgctttac
    16561 ctgttttttc caagacaaag ttttttacac gatccatatt ggttaccaag acgatcatgg
    16621 atgtttcttt gcctgctgct ttgatcgctt ctaagtcagc ttctgctaca agcgtatcag
    16681 gtgtcacttg ctgcccttct gttactttca catcaaatgg cgtaccgcct aagtcaactg
    16741 tgttgattcc catatgaagc aagatttcta aaccatttgg cgttgtgatc ccaatagcgt
    16801 gtttcgttgg gaagacagag ctgactaccc cagtaactgg agaatagata tgtccgtttt
    16861 ctggttctac aaagtagcct tcacccatca tcttttgtgc aaaaactgga tcacttgctt
    16921 tatctaaaga aacaactgtt ccgtcagcaa ctgatgagag gatagtcaca ctttcagctg
    16981 aagtttgagg tactactttt tcaggcactt catttactgt ggctgtttca gtcgctaatt
    17041 cttctttttc taaacggtct ttaggaatac cgaagaagaa tgtagcaacg aagccaccag
    17101 cataagctgc caataatcca aggatgtagc ctaaccattt accgtcagag atcaatggga
    17161 tcaaagcggc tcctgaaggt ccaatagcga tagccccgac atttccgatc attccgatca
    17221 ctgccccgcc gatacctcca ccgatacaag cagtaataaa tgggcgtcct agaggtaatg
    17281 ttactccgta aatcaatggt tcgccgattc ctaggatccc gactggtaaa gctcctttga
    17341 tcatttctgt taattcttta tcttttttgc agcgaatcca taatgccaat gctgcaccca
    17401 cttgtcctgc accagccatc gcaagaattg gaagcaatag cgtacgacca gtttcatcaa
    17461 tcatttgcac atggattgga gtcaaaattt gatgcagtcc gaacataacc atcggcaaga
    17521 ataatgtgcc tagaacgaag ccggcaaatg ctccaccaac gccaagtacc cagttgatcc
    17581 caccgaccat gccatcagaa atgaaacctg ctaaaggcat gatgaagaaa atttcaatca
    17641 gaccgatggc aatcaatgaa agagttggtg taacgatgat atctaccgca tcaggtacta
    17701 ctttatgcag ttttttctca acgattgaca atagccaaac agcgaagatg acacctagga
    17761 tcccaccttg tccagctgca agagcttcac cagtaaataa gtttttcagt ggtgcttccg
    17821 gattcatccc tgtcagcatc acaacggccc cgataactcc accaagagtt ggtgttgcac
    17881 caaaaacttg tgcagcattg atacctgtat agataactag gtagctaaac atcccgtttt
    17941 tcaagatgtt catcacatcg atatattgtt gccatgttgc tgcatcaagt gttccggctg
    18001 tgactaagtt tgctaaaact gcggcaatcc ctgccacgat cccagtaccg acaaatgcag
    18061 gaatcattgg cacaaagata ttagaaatgt cttttaatac cgatttgatt ttagatggtt
    18121 tttgtttcga tttttgtgcc gctttcattt cttgtgcccg ttcgtttaca cggtcttttc
    18181 cagatgctga tgaatttgct gtagctgtac ttacagaaga aagtttttct cctaatttca
    18241 cgcctgccaa atcaaccatt tgctgtgcta ctttgttcac tttcccaggt ccgatgatga
    18301 cttgaagctg ttcatcttct actacaccaa gcactcctgg aatcgctttt aattcagaga
    18361 catttacaag atcatcattg atgatcttca tacgcacacg agtcatgcag tggacaatgc
    18421 tttctacatt gcccatgccg cctgcccctt tataaatttg ttttgccagt tcttccaaag
    18481 tcagttcgtt ttttgccatg gtcttttccc cctcataagg tcccttttac aaatccgttt
    18541 gcttcagcta attttgcttg agcttcctct ttatcactat ttgtcaaaat catgacgata
    18601 gccagcttaa cattttgatc tgcttcttca aagtactttt cagctgtttc gtaatcagct
    18661 tctgtcgctt gcatgatgat tcgttttgag cgttcgatta gtttttcatt tgttggtttg
    18721 acatcgacca tcaagttatt gaatacttta cctataccaa tcatactgat agtagaaatc
    18781 atattcaaga taagtttttg tgctgttcct gacttcaatc gagtagagcc tgtcaaaaat
    18841 tctggacctg catcgacttc gattgggaaa tccgcgtgtt tactgatttc ggcttgttta
    18901 ttgcaagaaa tcgttcctgt gacagcaccg atcgattttg cgtaatctaa tccaccgatc
    18961 acatatggtg tacgaccgct agctgcgata ccaataacta tatcattttt tgtcaaattc
    19021 agcttgatta gatcttcttg ccctagctca taagaatctt cggccccttc gacagctaca
    19081 gtcattgctt cttctccacc agcaattaga cctatgacca tttccggttc tacaccgaat
    19141 gtcggtacgc attcagcagc atctaacaca cctagtcttc cacttgttcc ggctcccata
    19201 taaatcagac gtccaccttt tttaaaggac tcaatcgtgt tagcaatcac tggttcgata
    19261 cttgtcagtt ctttttcaac cgcagcggcc actttctgat cttctttgtt cattagttga
    19321 acggcttgag aaatactcat ctcatctaat ccaaaagttt cttggtttct gcgttccgtt
    19381 gttaggttct ctaaattgat tttgctcatt tttatcttcc ttcttttctt ataaattgat
    19441 agttcatacc agctttgatt tggttgatca atggtagctc ttctttgata actttagcta
    19501 ctacattcac tttttcatcc gctggcaaat catatttggt taattggatc tcacccatat
    19561 agcgcaaata cttttcgtta tctaaagtaa tagacccttt catacgcgta gctgtatttc
    19621 ttgctgggat aggcggaatc tctcgaaaac gtgcatctac gcttcgtatg acatctcttg
    19681 catcgtcttg ccgattcgta tgcttcccta atatatattc gtaaaattct tcatctacag
    19741 gttccgtatg gagcaagact gtctgttctt tttggaaaga agcaaattgc tcctgaacgt
    19801 tttctgacag acctgcatct ccgatgtaaa caatatccgt gttacttgct aatagatcta
    19861 gagcgcctgc aagaggatgg atccctcgat gttcttccaa agttggcaat ccttgatata
    19921 gtgggcctct caactctgta tctcctggta caaaaccttg gacaaggaga ccttgaattt
    19981 tgagccattg attttttttc tgataccatt ctttgtccag ccctgtttcg ggacgtggat
    20041 aataattatg ccaagcttca atctgtgaga aatcagcttc tgcttctttc aattcatcga
    20101 tatctttagg cgtgatcgtg ctagcattca aactgatttt caattgatgt gaccagtcgg
    20161 caatctgctg attggaaata tgataatcca ttcttaaacc tgttacacca attgctttca
    20221 aaggcgttag atcttctaat gaaaaacccg ctcgttccaa tgcctctcct gaaatatcga
    20281 ccatcaattc tagtttattc tttttcgctt cgcttcctag ttcgatcaat cgcttttgat
    20341 atgccgaaac atcatcttct ggtatatgca tggaagtgaa aataccgaca aagccattgg
    20401 atgccatttg gcggatatac tgtttcttct cttctgacaa agaatcgttc ataaatattg
    20461 aaaagccaaa catattatcc ctctttttct gtatccgtct tcatttctat gactagagta
    20521 tatatctaaa gaaacaaaat ttcaatactt ttattaaaat aattaaattt catttctaaa
    20581 ggttgagttt atattatcaa aagaattgtc ataacctaaa aaaggcactg ctaagcaaaa
    20641 gcagtgcctt gaaatatttt atttaaataa cacaaaaaca taattactca taagaaacca
    20701 attctgtatt atctaccatg aatgtaaaga acccctcatt aggcatcgga tgaaaataag
    20761 cattgatcac tcttatcaag gcattatggc aaactaacag atagacattt tcttcgtctt
    20821 cgatgcattc tttcaataaa ggaacgatcc gtgcatacac atctaagact gactccccat
    20881 ttgggaatcg tacagcgaac gctaaacgtg ctttttgaaa gttctcatct ttgcttggca
    20941 gtccatcata atccccaaaa tccatttcta ccaatcgttc atcaacggtc agcggcaagc
    21001 tgagacgatc agcaactgct tgtgctgtat ctcttgctcg ttgaagaggg gaatggatga
    21061 ttttcgtgat tggaacaggc aaatctacta ccttttctgc tagtttctgt gcctgcatct
    21121 gtcctttttc agtcagcggc acatctgccc gtccgcaaac taatccttgt gcgttccaag
    21181 ttgtttctcc gtgtctcgtt acataaagca tgtgattctc tccttcgttg gcaaagttcc
    21241 ccttaaccat accttgttgt tcttgagatt acaagggtta gattctctca agagaaataa
    21301 tctcagtaga tactttatcc aggaaggttt gatcatgttc cactagtaac atggcaggtt
    21361 tcacgttcaa gatcagttgt tctaattgtt cttgattgaa gacatccaaa taattcaatg
    21421 gttcatccca tgtatatagt tcagctggct gtgataaaga tttagccaat tccacttttt
    21481 tccgttggcc catactcatc tgctcgatct tgttatgaaa aacatctctt tccatcccaa
    21541 gctttcggag gttgttcaaa aatgcttggt agtctacttg gtttttctct gcaaattccg
    21601 ccaacgttcc tcgattgtct tcataatttt ggcttgcata actaatgctc agatgttttg
    21661 gctggtattt ttctcctata accttgccgt tgaatgcccc cagaagataa tggataatgg
    21721 atgactttcc tgcaccgtta ggacctgaaa tcgccacccg ctgatgaggc tcgattgtaa
    21781 aatgaattgg ctcgaataac agattttcat accctaattg aagatcttct acgcttaaaa
    21841 gtcgcttatg gtgagacgct tggctattca tcgtcaaaga atcgatatat tcgatatctt
    21901 ttaatagttt ttctttctcg ctgatctggg tctccatccg attcacgatt gctttggatc
    21961 gtttcatcgt ccgtgcagca tcagcaccca ctgctccttt attcactcgt ctagattcag
    22021 tatcgatgaa tccgacttgt ttctttgttt tatctccttc tcgggaacga gaccattcgg
    22081 ctttttcagc tgctgttttc tttagcctac tgacttcttt tttcaatttt tcattttgag
    22141 ccatttcaaa ttcatcacga agttttttct gttcttcata gatagagaaa ttcccttgat
    22201 aaagttccag ttgacttttt tcgattgcta aaacatggtc cactacttca tcgataaatc
    22261 cccggtcatg gctgaccacg atgaagcctt gttttttctt tttcaaataa gccgctactt
    22321 gttttctacc agagatatcc aaatgattcg ttggttcatc gattaacggg aaatgagtgt
    22381 catccacaaa taaaagtgcc agtaggactt ttgtcttctc tccccccgat agtgtgctga
    22441 attctctcca taagatttca ggatctgttt gcatcaattg gagctctctt tcgatttccc
    22501 atatctcaaa atccgtaata tcatttaaca cgtaataggt taaacgttct ttatcttttg
    22561 tctgttgcgg gaaataggca aattcttgct gatggattac ttgcccttga taaggtagtt
    22621 tgttttgtag aatattcagt aaagttgtct ttcctcgacc gtttcgtccg ataagtccta
    22681 gtttccattg tgtgtcaaaa tttagatttg cttgttcaaa taataatgtg ccttggctgt
    22741 cgtagccgaa tgtcagattt tttatttcga ttttagacat tttccatcac ctcgtaaaaa
    22801 aagaccagtc ctattttcag aactggctaa agaggatgca agggttggat acacacaaaa
    22861 agacccatac atcgaaaaaa ccaagccaat cccgaaaatc tgcacactgc ttccttgtct
    22921 ccacaaggta cagtctgaat gcagctttca tcaatcgact taatttttca atgcacggat
    22981 ccctcacttt gtcctatatt tgaaattacg atagcataaa gaaaatgatt ttgtcaacta
    23041 cttcgggagg atggtgaaaa atctgctaag tgtctgtcct aagatttttg ttttttctaa
    23101 tgtttgcctg tgttccattg cttcttctaa acctattgga cccaaatgaa tacttaagac
    23161 aattgggaga aaatcagaaa gtccttctag ctcttggtct ctacttccgc aaatagcgat
    23221 tattggtttt cgatattttt ttgcaagcac tgctacaccg tatggaactt ttccgtggat
    23281 cgtttgctga tcgatccgcc cttctcctgt aatgatgaca tctgcttgtt cgatcttttt
    23341 ttccaatcca gtgatttggc tgactaaatc gaaaccagaa accatcttcg ctcccaacaa
    23401 aaaaagggct ccaccaatac cgcctgctgc tccacttcct ggaatatcat ctaaatccac
    23461 ctcccagaaa tcttttattt gctgagcaat ctttcctgct tgtttatcta agaaagctag
    23521 cgtcgtatct attcctcctt tttgtcttcc aaattgccaa gcagctcctt gttgacctgt
    23581 atatggattg gtaacatctg cagcgataat caattttact ttagaaacaa gaacgtttct
    23641 tcgatcaaaa tgaagttttt tagtagataa taaaggattt ccttctgtgt acccttctaa
    23701 agaagcaccc aaggattgga gtaatcctag accaccatcg ctacacccgc ttcctcctaa
    23761 agtgacaata atttgttcta cttcttgaag cagtgcatct ttaataagtt ctcctaaggc
    23821 ataactggaa gcaagtcgga tagtttgatc cgttggttta tcaagtagat ctaaccccaa
    23881 tgcttgtgca gattcgatga ctgcggtgtt ttttccttgc caagttgtca ataaatagct
    23941 gatttttcgc tgacggaata agagatccat cccatcaatg gtacgccatg ttcccctgag
    24001 cgcgtaatag attgcttctg cagtattttc tccgccatct gcaatcggca cattgatttt
    24061 tcttccttta cgatcttccc agccatttag aactgcctga tttagttcaa atgaagaagc
    24121 acttccttta aatgaatcaa ttgccgccaa aatatccatc tgtatccctc actttcttct
    24181 taagtaagac actttttttc atttttttca aacaaaacaa tgctattttc ttccattttt
    24241 ctatatacta aaagggaact cattgaataa aggagagaaa aaacatgaaa atcgcaatag
    24301 ctggggccgg agcaatgggc agccgaatcg gattgatgct acatcaaagc ggaaatgagg
    24361 tcctcttgat cgatcgttgg cctgctcaca ttgaagccat tcgtacaaat gggttgattg
    24421 ctgactttaa cggtaaagaa gtggtcgcaa aactgccaat ctattcacca gaagaaatca
    24481 tcgaaagcaa cgaacacgtg gacttgatcg tagcattaac aaaagccaat caattagatg
    24541 atatgttttg ttcgatccaa tccattatta ctgacaatac ttatgttttg tgtttactaa
    24601 atggattagg tcatgaagat gtactggaaa aatatgtgcc aaagaaaaat atcttatttg
    24661 ggatcacgat gtggacagct ggtctagctg gtcccggaaa agtcacattg ttaggtgatg
    24721 gagaaatcga actagagaac ttggaaccag aaggcgaagc atttacaaaa aaagttgtag
    24781 aagtctttca agaagcaaat ttaaatccga tctatagcca taacgttcgt tattccattt
    24841 ggcgaaaagc ttgtgtaaat ggaacattga atggtttatg cacgattttg gattgtaata
    24901 ttgctgaact aggcgcgcaa aaagcggcag agagcatggt tcgaacaatc gtatctgaat
    24961 ttgcttctat cgcagcaaaa gaaggaatca tactagatca agaagaagtg taccaacata
    25021 ttgcttctac ttatgatcct gacaacatcg ggttgcatta cccatctatg taccaagacc
    25081 tgatcaaaaa ccatcgtttg actgaaatcg actacatcaa tggtgccatt tggcgaaaag
    25141 gacaaaaata tgatattgct acaccatatt gtgccttttt gacccaatta gtccatgcaa
    25201 aagaaggaat ccttggcgca gaataaaagg ctctctgatg agaatcagct gaatcagcag
    25261 ctggttctct ttttttaagc ccaataaaag atcaaaaaga aaacacaact ctcatgatgc
    25321 cataagaatt gtgcttgttt gtttcagtac ataatctctt caaaatgtag gttttttatt
    25381 tgttttcaac agaggagagg tcatattcgt attgctcata aatagtggaa tcgttagtga
    25441 taaatcgttt tgcccaattt tctgcatcat aaaattccaa tagctctgat aggattggct
    25501 taaatggtgt atgcagaaat tccggtaatt catctaaatc acgttctcga agcaaaaaaa
    25561 tattttcagg acgataaaaa tgataatgag caatcgcttg attatccgtc actagtttct
    25621 tttgaaaata catggattca aatgtccgca aagtcactcc ttgctgaccc tcccgcaaca
    25681 gttccagaat cccgcgcgat tcccctgttt ttttcaggta ctcttcataa gtaagatatc
    25741 caggttgttc attccctcga tgtggaagaa tgaaatagtt ggtcgtaaca tctcttttta
    25801 taaattcctg cttatagctt tctgctcgtt cttttctccc attattcgtt gcaccaaaaa
    25861 atagatccgt ctttggttcg ctagaagaaa aattcatatg tttcgaataa aatgaactat
    25921 tatgtttcag cccaagttct tttgcttcca aaaaatcaaa atgataaatc tcatcgattg
    25981 ccggttcatc ttgtgcctgc ttcagcatcg cctcgtgttc tgctttgaag tgattccaaa
    26041 aaaataacac aatttttgca ttggtaaatg ggcgcacgcg tcgagccatc tccatatcaa
    26101 atccataatc taaaaaaacg atcagctgat aatttgccaa ctgttgtcta gctgatttaa
    26161 tgaattccga ctgtagtaaa tcttttccgg accaaacatc taaagagcgg atggaaggca
    26221 aaaaataaag tgatttattt tttgcaacta gcagtacgtc atttttgttc atcttttccg
    26281 cctcactttc tctgtcactt tttcctatga tagcctgttt aaatagtaaa aagccagtaa
    26341 aacgtttcct atatttgtat gaaaatttaa catttgtatg aaaatatgat gaaataaatc
    26401 attcttctat taaaattcgc ctaaaagttt tctgaatagt tgtaattgtc tgaaaatttg
    26461 ttatcattag tcattgttaa atattgctat ttattcttaa tataaatttt gattgtgaat
    26521 ttaaaagaaa gaggtcattt atggaagata aaaaattgaa aaaacaactt agctccagac
    26581 atatcaccat gttagcttta ggtggagcaa tcggtgcagg tctatttaaa ggaagtggcg
    26641 aagcgattgg gatcgccggc ccttctgtat tgattgcttt tttgattggt gggctgattt
    26701 tatatatcgt gatgaaaggt ctgggaaaaa tcgttttaag cggtggcgac acgcatcacg
    26761 gattatctgg tttgatacgc ccttatctag gtgctcattc tgctgatttt actgattggg
    26821 tctactggtc tatgtggatt atcaatatca tcgctgaagc tgttgcagct gcttcctttt
    26881 tgcagttgtg gttcccgcac attccagcct gggtatttgt ttttatgttg gctgttttaa
    26941 ctacgatcat caatctttat tcggtacggt tattcgcaga aacagaatat tggctggctt
    27001 ttgctaaaat cagtgtcatt atcttactga tcatttttgc tacttatctt gtcggtcagc
    27061 aaatgttggg cacaggcgtt ttccctaccc ttcagcaatt gactgaccat ggtgggttta
    27121 ctcctcatgg tatgaaggga atcatcagtt ctttgcttgt agttatctat tcttacggcg
    27181 gttctgaact gattgctatc acggtaagcg aagcagatga tcctaaacgt gcgattccta
    27241 aagcaatcaa aggtgtcatg ggaagaatca tttcctttta catcattcct ttgtttttgc
    27301 ttttgattct ttttccttgg aatactttag caggcacgaa tgtcagtcct tttgtgatgg
    27361 tatttgaaaa aatgaatatc ccatttgcag cggatatcgt caattttgtg attgtccttg
    27421 ctttgttctc atctatcaat tcgggtgtgt atgcatcttc aagaattttg ttcttccgtt
    27481 tgaaggatcg aaaaggttcc tctagaaaac tagctgtttt aaacaagcat caagtaccgc
    27541 aacgtgctgt tttattttgt accagtgttc tttatcttgg tgtagctctt tcttattttg
    27601 tcggtgacaa attatttggc tatctggcag gttccctttc gtatacagta ttattgatct
    27661 ggatcattat cagtgcggca tcctttgtac tggcactaag aaaaggaaca ttatgggata
    27721 aaggaatcag cttttttgca ttagctgtct taggattgat tttccttggt atcctgttca
    27781 cgaactccat tggggtaact gtattgactg gcctgcttta cctgttcatt tatttcagtt
    27841 atcaaaaaaa gaatgatgcg ttcgtattga cgaatgaatg aaatcgatag aaaattttat
    27901 tttatttatg tggaatagat ggagctattg aagaaaggag ttgtgacaaa agtcttgttg
    27961 caactccttt ttccgaataa acagtctcat gacctcttgg taaacggtgt tcaaaaaccc
    28021 tctgtgcaaa ataattgatt gttctccatg ctcatgacat gatagaagaa aggaatgaaa
    28081 aaaaggagtt tgttttaaat gaaaaagtat tggaagtttt tgttgatgat cggtgtttca
    28141 acggtagtta tgtttggtat gatgtacttg aatgtctatg cgctcgatca tatcttcttc
    28201 agtcgtacac gagtattcat ggcgttgatg atgggtgcag tcatggcagt catcatgctg
    28261 ttgttcatgt ggaaaatgta tgaaaacaaa aagataaatg ttatgatctt agccgctagt
    28321 cttcttgttt ttgccggttc cttgtttatg gtacgaagcc aaactactgt caacgacaca
    28381 gcttggatga aggccatgat tcctcatcat tctatcgcta tcctggcaag taaacgagct
    28441 gatttaaaag atcctgaagt aaaaaaatta gcggaagaaa tcattcaggc acaagaagaa
    28501 gagatcgaca aaatgaaaaa aatgatcaaa gaaatggaat gaccattatc cgtccaaata
    28561 aaaaaagagg atgttccaga agttatcagc ttcaagaaat aagaagaagc tacttctgga
    28621 acgccgtgaa taaggggttg tgaaaaagct gccctgcatc aagtaataag aacagccaaa
    28681 caaaaatagc ttctcctatt tttcgtttgg ttaggcttat taccgcagat gcaagctttt
    28741 gaacaccgtt tattaagagg ttgtaaaatc agcgttctga cctgagtatt agagagacaa
    28801 atggaaaaat agttcctcat attttgccat tttttgagct aataccgagg gtcagctgat
    28861 tgaacaccgt ttatcgagag gttgtgaaaa agtcgcttag tttcgcgtat taagaaaaat
    28921 tttgtgaaat ggctttccac atttttgtat aaatttggct taataccgag aaactggctt
    28981 ttgaataccg tttattcgta aaagggattg tgacaaaagt cttgtcacaa tcccttttta
    29041 tgtttttatt gttctaattg cgctgtcttg cttactgctt cttgcggttt cacaaatttt
    29101 tttgagattc cccaacctgt gaaggcactg accatcgaaa acacaggaga aagcaaactg
    29161 aaaaatacga atggcaagta agtgagtgta ggaataccaa atgtacttgc tacgaaactt
    29221 ccggcaattc cccaaggcac taagtagttg attactgttc caccatcttc tagcgttcta
    29281 cctaatacag ttggatcaaa tcctgctttt tcataagttt ctttaaatgc attaccaggc
    29341 agaataacag ataggaattg ttcaccaata aaaatattta ctccgatact tgtcatcaat
    29401 gttgtaaaaa tcaaatttgg tgtgttcgtc atctttcttg atactcgttc catcacggtt
    29461 gagatcaagc caaattcgat caacaatcca cctaatgaca aagttagaat aatcaatgac
    29521 acagtaggca tcatactttc gattcctccg cggctaagca gtgaatcgat ttgttggttt
    29581 ccagtatttg aaacaaaccc tgattcgatg accccagcca acttcgttaa ggaagtttgt
    29641 gggttttgaa taaaaatcat tcctactgct acagcgatat tcaacaaaat cgttaaaatt
    29701 gccggcacct ttttccatgc acacagtaac attaaaaata aaggaatcag tgaccagatt
    29761 gaaatagtaa agtttgcttc tagaacatca gtcacttcac gaatagatga aagacttgcg
    29821 gctttacttg tgtgtcctaa aatcatgaat aaaaagaaag aaacaacaaa tgccgggaca
    29881 gttgaccaca tcatatgctt gatgtgtttg aacaaatcgg catcaacaat agctgctgct
    29941 agatttgttg attcggataa tggagacatc ttatctccaa aaacggcacc tgaaacaata
    30001 gctccaacaa ccagcgctgg atttaagcct agtgttgtcc caataccgaa aaatgcaatc
    30061 ccaatcgttg acataacagt aaaggcactc cctacagcgc tacctacgat tgcacacaca
    30121 acaaatacag aagggacaaa ccactggaca ctgatcaatt tgaacccaat gaccattaga
    30181 gttggaatga tccctgcttg tatccataca gcaatcaatg aaccgactaa aataaaaata
    30241 aagattggga tgattcctgg tttgatgcca ttgatgattc cttcgtgcag ccaatctgtc
    30301 gggtatcttt ttatcactgc atacaacatg accattccga tcgctgctaa gatcgtgata
    30361 ttaggtgaga cctttaatcc gacaacgcca atcgcaatgc ctaaaacaat aaccattaat
    30421 acaagtgttg cttctaaaaa acttggtgtt cctttttttc ccatgataat ctctcctttt
    30481 ttagttgttc ttatgttcta aaaaagttaa gatatcggat tactccgccg caattcatcc
    30541 gcgattcctc ctttataaaa ataaaaagac ctgttttaaa ctaaggacga atgactccgc
    30601 ggtaccacct taattaaaaa cagatctgtt tttcactttc aaacttttat tcaaatgtat
    30661 ggactttcca tgtaattcgt caattaggct tgatcggttc acagcgacca ccgactttct
    30721 gaacaaaaca cctaaccgct actgcgtgaa aagttgactt gtacgaagtt taccttccat
    30781 aagatcattt gtcaatgaaa aagaaaatat tttttcattt ttctgaaaat tatttttata
    30841 actttcacac acaagtttat tttttactag aaagtagcgc cctcttgttt ttttcttgct
    30901 actatttctt gatgttcttg cttgattctt tctaatagct ttggctcttt caacagatca
    30961 agagctgtat tggcgagtag ttctgctgca atcgcaatag aagcaaggcc tttgtcactt
    31021 ttagcagcag ctttgaattc ttctgtgtgt ccggcaatat atgtctcaga gattgctacc
    31081 gtaggctgga ttgttggtat gacttggctg acatttccta catcagaaga accgagactt
    31141 gttctttgtt ccgtttctat ttcttcatct ggaacacctg cttcttttgc atgctggaag
    31201 aataagtcat caaaagaagg agtaacgatg acatcatcaa ctgcattttg gaacaatcca
    31261 aactcaaaag tcgtgcccgt acttatcgca gcacctcgta cgatattttc cactttttca
    31321 tatactttat ctaatgtttg ccgatttcct gcacgtaaat agaaacgtcc agcagcgtaa
    31381 tctggaatga cgttggcagc tactcctccg tctgtaatga tcccatggat attgacatct
    31441 tttggcaatt gcaagcgtag agcgttgatt ccattaaaca cttggatcaa tgcttccaaa
    31501 gcatttactc ctttttcagg tgctgctgct gcatgggaag cgacaccgta aaatttgatg
    31561 tcgactggat cattcgctaa agattctgtc gttttcccat aacgaaaagc tggatggacg
    31621 cataacgcta catcgacatc ctcaaaaaaa ccttctcgaa caaagctgcc tttagctgat
    31681 ccgttctcgc ccccttcttc ccctggggtg ccatatacac ggatttctcc accagtttca
    31741 tcaatgaatt gcttcactat gctggcggct aatacggagt aagtaccaaa caaattatgc
    31801 ccgcatgcat gaccaatacc tggtaaagca tcgaattctg ccaaaaaggc aattgtggga
    31861 ccgggtttat tggaaatata acgtgcatca aaggctgttc ggtgtccagc tacttctttt
    31921 tctacccaaa acctttcttt ttctaattgt ttaatcaacg ctgctgagga ataaaactca
    31981 tagttactta cttcaggatg ttcgtggata tctagagcaa gtgcttgata ttctggtaaa
    32041 cgatattgga ttagatcctt caattggttt gttgagattg ttgttgtcat aaaaaattcc
    32101 tcctatgcta gtcatttttc ttattttctc ccgaatgttt cccatgcagg cacgcttgat
    32161 ccttttgatg ttttctcgat tactttgact gtgtcttctg tttgataagc gtctactatt
    32221 ttttgatacg ttttattgtt ttcatcttct tttcttgcaa cgatgatatt cacataagga
    32281 cgtgcatggt catccactgg ttctaagaag atggcatcag aagcaggatt aaatccagca
    32341 tctacagcta caccgctatt gatcactgaa atgtctacat ctgacaaagc acgtgctgtt
    32401 tgggaagcat ctaattcaga aatgtctaga ttttttctat tctcggtaat atcagaaaca
    32461 gtgggcgctt gtccttttgc cttatccact ttgatcaatc cagcagtttg caataacaga
    32521 agagctctgc cgccatttgt aacatcatta gggattgcta cttttgctcc gtctttgata
    32581 tcagctacat ccttcaaagt gttagaataa attcccagcg gtgcattgac agtatttccg
    32641 atcgatacta aatcagttcc gtgttcttta ttgtaattgt ctaaaaagaa ttgatgttgg
    32701 aaagaattca aatcaatcga tccatctgct aaagccacat ttggttgatt gtaatctgta
    32761 aatttcacgt attccaaatt aatgttctcc tttttcaaac ggtctttcac gttgtcccat
    32821 acatctgtat cttctccaat aatccctagt tttacgatag tttctttttt gtcagttgta
    32881 tctgcttttc cttggctaca ccctgccaac caaataattg ctacaaatgt aatgactaca
    32941 aaacgaataa tttttttcat tttttctcct ccactttcaa tgtgtaattt ttttaatcag
    33001 ttgattactg ataaattgac ttaaaaaaac taagattaga atcaataatg tagccacaat
    33061 cgttacatct gtttggaatc gattataccc tctcgatata gcaagattcc ctaatccgcc
    33121 tgcacctact gctccagcca tggtagtcag tccgatcacg ttgatgatag tcaacgccga
    33181 gacccgaatg ataccaggca aaccttctaa caaatacaca cgtaaaatga taccgattgg
    33241 actggttccc atcgcttctg ccgcttcgat caccccagga tcaacttcta gcagtgcgtt
    33301 ctcgatttga cgagcaaaaa acggaacaac tccaaaaatc aatggcacta aagctgcttt
    33361 cgcaccaatc gtcgttccaa ctaagaaacg agtagtcaat gctaaaagag aaagcaagat
    33421 aatgaaaggg atggaacgaa tgatattgat cagcttatca agtatttcaa atagcagacg
    33481 gttagctagt aatccattag ggcgtgtcac tactaacgtc acacctaaga tgattccaat
    33541 catcccgcca atgaatgctg tccaaaaaga catatatagt gtttgcttcg tcgcttcgac
    33601 aaattcatcc ggaatcaaat aaacattggg caaatatgtt ttaaagaaat cagtcatttt
    33661 atcttcctcc ttcgatcact ttgatacgac tatctaaatc gtttttttgt aaaatattta
    33721 tttttacacc agatttgatc aaataatcca atgctttttt ccgctgtttg gtttctccag
    33781 ctaaagtaac gatcaaactc cctaacggcg tttcttgtaa taactctaca ttgccatata
    33841 aaatattcgc tgttacttga tatttgctgt acagatgagc gattagaggt tcgttcgtct
    33901 gatttccaat ataacttaat tccaccagcc atttattggc gatctgatcg gcaaaagcag
    33961 aatgggcaat gatcgtttct aatgcttggt caagatgagt agctgttcga ataaaatctt
    34021 tggtcaattc ttcttcagga ttgctgaaga tctggatgct gctacctttt tcaacgattc
    34081 ttccatcttc cataaccgct accttgttgc agatttcttt gacaacttgc atctcatgag
    34141 tgatcaggac gattgttaaa cctaactggc gattcaattt ttttaacaat gcaaggatct
    34201 gaagggtcgt tttcggatca agtgcactgg ttgcttcatc acataataat accttaggtt
    34261 cattcgccaa agctcgtgcg attgctactc gttgtttttg tccccccgac agttgtttag
    34321 gataagcaga tgctttttct tccaaaccga caagttccag tagttcattc actttttgac
    34381 gacgttcctg tttcgatttt ccagaatact ttaaagaaaa atcaacgttg tcaaaaatag
    34441 ttcggctttc cattagattg aaatgctgaa agatcatacc gatggccttt ctttcttttc
    34501 gcaattcttt gggagagagc tgactcagat tcgtctgatt gacaatgact tctcctgctg
    34561 ttggtttctg cagcaaattc atcacacgaa ctaacgtact tttccctgca cctgagtaac
    34621 cgacgattcc ataaacatcg cctttttcaa tcgttaaatc tacagaatct actgcctgta
    34681 tctctttttc tttttgtttg aaacatacag aaacatctcg caattcaatc attaaaattc
    34741 ctccttatct aaaaaaagcg tcctcaagaa aatgaaaatg tcattttctt gaggacgctt
    34801 ttttgcgtgt taccacctca tgttcaagct gtgttcacac acaactcctc ttcaagtacg
    34861 atcatactct agcaagatat cggtcgcagc cgtcatcgcc ttacatctat gctcgggatg
    34921 aattgctcga tggccatttt ccaatagtcg ttccttgctt cttctcagct accgaagctc
    34981 tctgtcaaag gttcttctat cgtacttacc atttcaatgc aaataatatt caaaaaaaaa
    35041 gtccttttac taacataaac tatgtcagta aaaggacgac gtatcgcggt accacctttt
    35101 ttcggaagtt tccttccctc aagcccaaca tctcaaaaag agagattggc gttcgtttta
    35161 acggtcgaac agttccgaat ttgcctactt atcgacaaat tattctccaa ggccattttc
    35221 agcttttctt acttgactct tctcaccagc cagagcctct ctgcaaagtt tccaaactta
    35281 ctttcctttt caacgaaatc gatattttgt tgttgggatt aatctacatg aggaaaactg
    35341 aaaagtcaag tacgaaattc aaattatttt ttctatattt atttttctgt ttttttattt
    35401 aacatttcaa taacagttcg aagatctttt ccttccgcaa gaagtttcaa tttttccttt
    35461 tcttcgacgg tataatttcg gacaaaactg gaatcaggta tcaaaacaac gcctaatcct
    35521 gctcgtgaag cagctgttgc tcctactaaa gaatcttcca ctgcaaaggc tgaactagct
    35581 tctaatcctg ttcgttccaa agctgtcaga taaggaatcg gtgaaggctt gttctccgac
    35641 acttcgtctc cgaatgtaaa cgtgtccaag taatcagcta atttaaagtg atccaagata
    35701 tctgttgctc ttttcgtaac agtggtcgta gcaatgccta gtttcatatg acgttctttt
    35761 aagtctgcca atatttcttt tgcatatggt tttaagtgga tggctccgct tgtcagctgt
    35821 tgataaaaat attcttcacg cttcgctcgg acttcctgta ctttttcagg gctacctgct
    35881 gctttcacta aaatatcaaa actttgtttc cagttttttc cgccccaagc gtcaacgatt
    35941 tcttttggga tatggatatc aaattgctga aatccccaaa gccagccatc acgatacgtt
    36001 ttttctgtgt caactaacaa accatctaaa tcaaatattg cacctttcat ctatacatta
    36061 atcctttcca aaacaataca catagtttag cacgactggt ccaaaaagaa cagaagcaaa
    36121 gaaccagcct tctcgtttat aagagttctt ggaatcgacg gatatagatt ttcttcacag
    36181 ctgtcacaag cgccagataa gcgatgactg tagcaaataa ccataaccag taattgccag
    36241 gaagtggcat caagcctaat tcagcaccaa aacttgtaaa aggaaggaca gaaccgacaa
    36301 taattcctaa tgtagtgata acaaacatgg caaatgttgc gttgctttgg ataaatggta
    36361 ctttcggtgt acgcagtgca tggagcacta aggtttgtgt ccatagtgat tcgacaaacc
    36421 atcctgcatg gaataatgcg atgaaaatca ctcgctgtga actatccaat gtatggaaac
    36481 ttccgcccac tacagcagga caaataacaa aatacatcaa caagtaagta gtaatatcaa
    36541 atatcgaact tgttggacca aagtaaacca tgaattcccc aatactagat gcattccatt
    36601 tttttggctc gtccaaatat tctttatcca tccgatccca tggcaaggaa atgcaggaaa
    36661 catcatagat cagattcaaa aacaaaagtt gtaaaggcag cattggtaaa aatggcagga
    36721 acgtacttgc aatcagcacg gaaaacatat tgccaaaatt agaacttgct gtagccttga
    36781 tatatttcat gatattccca aaagtttctc gtccagaaag aaggcctctt tccaaaatcg
    36841 tcaaatcttt ttctaaaagg atcacgtctg cagattcttt cgcaatatcc acagctgtat
    36901 ctacagaaat accgatatca gccactttca ttgcaggagc gtcattgata ccatctccta
    36961 aaaatccgac agtatgacca tttttacgta aagctgtcgt caaacgtgct ttttgctgag
    37021 gattcaattt tacgaatact tcatggtctt cagctacttg tgctaattca tgatcactca
    37081 tttccgagat tttctcaccg gtaaccaatt cattgatctc tagacctact tgacggcaca
    37141 ctgaacgtgt aactaattcg ttgtccccag tcagaacttt tacagctaca ccatgttctt
    37201 tcagagcttt caacgcttgt ttcgttgttt cttttggtgg atcaaggaaa gcaagataac
    37261 caatcaaaac catgtctgac tcatctttga ctgaaaattc tccaacgacg cttggattgg
    37321 tcttttgtgc gacaccaatc actcgtaagc catcttcatt aagtgactgt acttttgcaa
    37381 gtacggcttc ttttcgttct tttgttaatg gtgtaacagt accgccaaga tcaataaatt
    37441 tagaaatgtt cagcatttct tcaatcgcgc cttttgtgat catttgtgtt ttaccagctg
    37501 gatcagcaac cacaacactc atacgacgac gttcaaaatc aaaaggaatt tcatccactt
    37561 tttgataatt gatcttatct gtatctaaag tttctgttgc ttcatcaatg atagccacat
    37621 ctaacaaatt tttcagccct gtttgatagt agctatttag atacgcatgg cgcaaaacac
    37681 gatcatcttc atttccttca caatccatat gatattcaag aatgatttta tcttgtgtca
    37741 atgtccccgt tttatccgta cataacacat cgattgcacc aaagttctgg atggcattca
    37801 aatttttgat cactgttcct tttttggcca tcacacttgc tcctttgacc aaattcgtcg
    37861 taacgatcat tggaagcatt tcaggtgtta gaccaactgc tacagatagt ccaaaaagaa
    37921 aagcttctag ccaatcccct tttgtaagcc cattgatgac gataactgct ggagccatca
    37981 atgacataaa tttgatcagt agcatggatg ttttatggat gcctaattca aagcttgatt
    38041 gaattggttt ttcagatagc gtttctgcta catgaccaaa tagtgtttca tttccaacag
    38101 aaacaatgat tccttccgca cttccactga tgacattact tcccataaag acaagatttt
    38161 cgtaacttgt ttcgctttgc tgatcgtctt cacatacttt cgcatgcttt tctactggat
    38221 agctttcccc tgtcaatgct gcttgggaaa tgaataaatc ctttgcggac aataaacgca
    38281 tatccgctgg tatcatatcc cctgctgaaa gccgaacaag atctccacag acgatttctt
    38341 ccataggaat ctctgtgtac tttccattac gttttacagc tgccgttact ttcacaagtg
    38401 atttcagttt ttcagccgct tgattggaac gtacggactg aatcaacgtc attgtcccgc
    38461 tgacgataac catagtgaag ataatggcta caccgaaaag atctttatct ccaggagctg
    38521 ctaatacata ttccgtgatg aatgaaataa ttcctaatgc gatcaacact aatgtaaatg
    38581 gcgtgatgta tgctttcaat atttctacaa tgaatggtgt ttttttgcca tatgaaatcg
    38641 cattttcacc gtaattttct cttgaacgtt cgatttgttc ttctgtcaat ccatttgctg
    38701 atgcatggaa tagttcaaat aattcttgtt tcgtttttcc agcaaaagct gcatagattt
    38761 tttgtgtatc tttctttttt tccatctcaa tctctccttt gtgtccaaaa gacagctgtt
    38821 ctcgctgctt ttggtcttta gctattcatc ggatcgatag agaaaaaact gaaaggggct
    38881 gagaacagat agataatagc cctggccttt cctctagatc cttgtttttc gaatgactgt
    38941 catccatttg ttctcatctc ctttactaag taattttaat tcatgacttc caatcatttt
    39001 tcgaatagtt atttttccaa ttagaccacc tcctttttat ttttcaccga tggggatttg
    39061 cggcaacaaa aaaacctctc gccaaatact cagcgaaagg tttgaaaaag catattaaaa
    39121 aaagcttctc agatctcatc gttgagtttt gacactgtac aacgtaaaaa gcagatacct
    39181 gcttttagct accttatgaa aagccttatc tcgacaattc ctgttctacc cattggcgtc
    39241 tctcgacgtt tttgggcagt agcctatgtt tgtataggag cctcacctaa caaatcttat
    39301 tcggttttcc ttcgttaata taacaactaa aaaagaggat gtcaaatcaa ttttccatgt
    39361 ttttcatact ttttttaaaa tacacacgtt atattcctgt ctcacgcggt tttatttgtc
    39421 tcattttcca cctcccgcta taatagaaat atagtaggaa acgaggtgct gttatggaaa
    39481 gaacacttgt tattatcaag ccagacggcg tgagacgcca tttagtcgga agcatcatcc
    39541 aacgttttga agcaaaggga ctggccattg ctgaaatgaa gtttgaaact atgactcctg
    39601 aattagcaaa agaacattat cagcacttga ctgagcgcag tttttttgat gaactgatcg
    39661 attacatgac ttccggtcct gtcgtttatc ttgtgctggt cggcgaggaa gtgatcgata
    39721 tcgtccgtaa gatggttgga gcaacaaaag ctgccgatgc tgttccaggt acgatccgag
    39781 gagactacgc gctgccgggg actgaaaaca tcattcacgc ttctgattcc cgtgatgctg
    39841 cggtaaaaga gatcgcccgc tttttcccgc cattcgatat aaacaaaccg tctgaaaaag
    39901 cggcgaagtg atacaaattc taaactgctg gtctagttgc tgggttcagc aatggccagc
    39961 agttttttct ctaaggacct ccgttgattc tttcctaaca tatattcgag cactgtgaag
    40021 aattcttggt agtctttcgt cttttcttcc cctaaagcag cagaaatcaa tctttgataa
    40081 taatctgcca tcaatgtttc atagatcact gtacttcttt ctttatgtgc taagctccgt
    40141 ttcatatctt cattcacact acgtgccttt ttcaaccgac cattttctat ataaaacgaa
    40201 gtcaggttca ttaaaaaagt aaagagatac tgctcatgat tgttcaactg tgctaatcgt
    40261 cgcattcttt ttttgatttt caaataattg ttctcaatga accgatcact gaatatccaa
    40321 ggaaaatcag ctagaataga tagttcaaat tgaccatagt ctggtatctg catcaagtat
    40381 tcttcaatga ttcggcattc ataacgtaaa aattcaaatt ctgtctcaga tacttctgtt
    40441 ttttcgacca tgtgaagatt caaaaccaaa taatacagat cttctgtttt cttaaaatgc
    40501 ctacggttat tgctgatctc tttataagga tcaaagctgt cttcttgtac ccgatctagc
    40561 attcgtcttt ttttctttaa ctttattggt tcatccagct ggtttcgcat ccattcatat
    40621 tctgcaagtg tcatgttcat tcgctctaaa aatgatctca atgttacaaa tgacattttt
    40681 ttcactgaat ttgtctcaat cccgactaga gaagtcttaa aacagattcc ttccgctaat
    40741 tgggactgtg tcaacttctt gttgactcga agtttcttga tcaattcacc atcatacata
    40801 atagttctcc tttttaattt aaatttttcc acattcaata aatagatacg ttaaaaagga
    40861 aaaatacacg aaaaaaataa aaaaatgttt ttttcataaa aaataccaac cttcaagcat
    40921 tagaatgttg tctaactact gaaggttggt ttgattgttt attgattatt tttttgctgt
    40981 tcaacgtctg ttcttgttac tggatataag ttggttgttg ttttattacc attttttgaa
    41041 aagacacttg tcgaatgttc gcctttttgc ttttcgattg cttcattacg ttccgcttca
    41101 tttttatagt catatttttt cggatcaatc gtctcaaatc cgtttggcac ataaaaccgc
    41161 aagagatttt cctgattcag cttatctgaa agacttaacg cagtcttgac tttttcctga
    41221 tctttggaga tttcctcatc gattcctgct gctttcgtat cgatgatttc tcctgtctga
    41281 ttattgtaag cttttcctcc taggacggtg tatttgggtg tcacaaagtt cccatttcga
    41341 aaagcaacta cttgatcatg ctgagaagat aataagtctg taccaaaatg aagataatct
    41401 ttatcatcga ttcccaatag atgtagtaat gtcggcaaaa catcgatttc tccaccgtat
    41461 tcatgattga ctgtcccttt agtataacca ggcatatgga tcatcaatgg cactcgttgc
    41521 atctgtgcgt tgtcaaattc atcccatgta tctggatctt taccaagagc gcttgctaaa
    41581 tctttattat ctgtattcga gatcccaaaa tgatccccgt aaatcacaaa gattgaattt
    41641 tggtaaatac cagatttttt caaatacgta aaaaattgtt ccaatgattg atccagataa
    41701 tgtgccgttc gaacataatt atctactgtg ctattgccag tattcaaaga tggaaagtcg
    41761 aaattcttgt catccgtata ataaggcgtg tggttggtta cggaaagaaa tttcgtgtaa
    41821 aacggctgtt gtagatgctc aagatattgg gcagattctc tgaataaatc tttatctaat
    41881 atcccataac ctaacgtttc gtcggattca tcaaaataag atcgatcaaa gaaattgtca
    41941 taacctaagt ttttatatac atgatctcta ttccaaaaac tagctacatt cccatggaaa
    42001 acagcgcttg tatagccttg cttttgtcca aggatcgctg gtgcggcttg aaagacattg
    42061 tctgacccaa gttctgtgaa taatgagcct tgaggtaaac caaatgtgcc agtttccaac
    42121 atattttcag catcacttgt ctttccctgt cctacttcat gaaagaaatt gtcaaagctg
    42181 atcgttgctt gattctggaa aatactattt aaaaacggtg tgacttcttg tccgtctacc
    42241 ttcatattaa tcaaaaactg ctggaaactt tctaaatgga gaacgatcac atttttccct
    42301 ttagcgatcc caaatgtttc gggatttggc tctgcataat gtttctttgt ataatctaaa
    42361 acttcatcaa tcccattact gcttgcatgc gcgcgaacac tgctagtcat tcctgttttg
    42421 attccgtcat aaatagtata cgcatcgatt cctagatatt tgacaatata tgaacggtca
    42481 aatgttcgct gcagcaattg tggacggttg gcttcgctca gcgctagatt tcctgaaaaa
    42541 ataagccctg aaagacaggt aacagcgata gccatcggtt tgccgacagg gttgcttttg
    42601 ataggtattt tcttgacctt caaccaaacc agtagcccaa tgaacacagc gatatccagc
    42661 cagtagatga catcatgggg cttcatcaaa gaaaaggagc ttcctgacaa tccttgcgac
    42721 actttcgaaa agcctaatac agatttgact gtaatgaaat ctgtgaattc ccgataaaaa
    42781 ataatattca agtacaacaa aagcgtattt aaaatatcaa tgatcaaaag aatagctaac
    42841 gctggctttg gctttttaat atacaaagaa aggccaaaaa acaataaggt agtcgcaatt
    42901 gggttgatcc acaagataaa gtactggatc gtaccttcca cacctaatga aaaatctgca
    42961 tagtaggcaa tgatcgtctt cagccagaaa aagaaaacag ctgtcaaaac gattgctaaa
    43021 cgattttggc acaatttttt aagttgtgtc acgtatatca cttctctcta ttcacatttt
    43081 ttcttatttt gctcctgaaa aatgaaaatt tcctaaaaaa gttgttatct taagattttt
    43141 ttattctttc taaagattat tccgtccact tttcacagca taaaaccgct gcgatgtaag
    43201 cgattaaact aatgaagaga taaaagtctt gtggtgaccg cttcactcca cggtattcaa
    43261 aagccagttt ctcggtattc gtacaaaaaa agaggctgtg acaaaattct tgtcgcagct
    43321 ccttatttct tgatgcagga cagctttttc acaacttctt ccttaaacgg tgttctcctg
    43381 ccaactcctc ggccacaagt cacaatattt ataaaacatg agaagctgtt ttttgaaatt
    43441 gttccttgtg gctcagagtt aaacggcagg ctcaagacct cttgatacac ggtgttcaat
    43501 cagctgaccc tcggtattag attatgtcag gacgctaatt ttacaacctc ttggtaaacg
    43561 gtgttcaaaa gcttgcatct gcggtaataa gcccaaccaa acgaaaaata taaggagcta
    43621 ttttttgaaa ttgctccttg tggctcagag ttaaacggca ggctcaagac ctcttgatac
    43681 acggtgttca atcagctgac cttcggtatt agctcaaaaa acggcaaaat atgaggaact
    43741 attttgccgt ttgtttctct aatactcagg tcaaaacgct gatttcacaa cctctgtttt
    43801 tcattgtcca taggattgat attgtccttg tgatgactca ttcgtaataa ttgaggcttg
    43861 attttcttca cttcccgtta tcgtttgtcc actgccagaa tctgaaggac ttgttcctgt
    43921 cgactcgttt ttttgagaag agacagttgt tttcttgact tgttcatttg tctttttcca
    43981 ttccgctaaa cgatcggcga ccactttttt gtctactttt tcagaataag cggtgatcac
    44041 ttttccattt ttcaaacggg ctactttgtc gccatcatga gtcaaaagca aaacggtcaa
    44101 atgcttgtct ttaaattgat attgtctgag agaaatcgca ctaagtacgt catcgatatt
    44161 aataaattga ttttcttctt ttccttttgg caaataaatg tccaattcgc ctttttcgta
    44221 attggcagtt attttatact ccgcccacca ataagttgag aattcttttt tgaaattatc
    44281 tactgattct ttgcttgcta cgattttttc tgtttctttc tgagcaagat ctgctgtcat
    44341 ttttcctgaa aatgctgcgg ataaaataag cagtaagaaa aaaccgaata accaaaagag
    44401 aagaaacggt ctttttttcc tcatgctttc ctccactcct atttaatttt gactattcta
    44461 ttatatacaa aaaatgaaat agaaagacaa aaaagttgaa aaagatcgta aataattgaa
    44521 cttctttttc acaaaatggt aagaatggag aatttcacgc atttgctctc aactattctt
    44581 tcttaagcct caaatccgtt tttatctgat acgtctttga accaatgacc cgattttttg
    44641 attgttcttt ttccatcttg tgctaagtct actgaaataa agccatatcg attcttatat
    44701 gcattggtcc acgaccagtt atccatacat gtccacatat gatagcctaa acaattggca
    44761 ccttcttgaa tggcttgatg gacatatttc agatgagatt ggacaaattc gatccgataa
    44821 tcatcgtgga tcatgccatc ttctttgata aatcgtgctt cgttttctac acccataccg
    44881 ttttcagaaa taaagcagtc gatatttcca taatgatctc gtacattgat caaaatatca
    44941 tagattcctt gttcatagat ctcccatccg cgataaggat tcattttacg accaggcatt
    45001 tcataattgt cgaaataatc ctcaggcatt ggaccgtttt tagtatcgat tggtgtttct
    45061 tttgctttta cccggcgtgg ctggtaataa ttgataccta aaagatctac tgtattttct
    45121 ttgataaccg tcaaatcctc tgcatggatc acaggcatga gatcaagttc ttttactatc
    45181 tcaatcaatt cacttggaaa ctcgcctttt acagctggat caaggaatga acgattgaag
    45241 aacgcatctg ctatttgcgc tgctttaaca tcttcagggt ttgattcgtc tcttggatag
    45301 cttggtgtca gattcaagat gataccaata acaccacctt gctccatttc atggtaagat
    45361 ttgatggcta acgctgaagc caacgcttca tgatagccaa cttggatcgc ttttttcata
    45421 tctgactctt ctggataatg ccatcgatat aaataacctg cttcgactgg aacgattggt
    45481 tcattatgag taaaccattt ttttactttc gaaccaaaca attcaaaaca acttttggca
    45541 tatttttcat aggcttcaac cgtttctcgg ttcaaccatc cgcctttttc ttgcagatac
    45601 atcggcatat cgaaatgata cagattcaca aagggttcga taccgttttc aatcaactca
    45661 tcaaaataac gatgatagaa acgaacggct tcttcgttga ttgttccaga ttcatcaggt
    45721 atcaaacggc tccattggat agatgttcga aatgaattat gcccagtctg tttcatcaac
    45781 tgtacatcct cttgatactt cgtataaact tgcgaggttt tttcaggtcc tacttgatca
    45841 aagaactttt gcggttcttt tttgtaccaa taatcaaaaa tgttctctcc ttttccatcg
    45901 cctgaaatcc ttccttctgt ttgtgggcca cttgctgcag aaccccacca aaaatttgct
    45961 ggaaattgat attttttcat caaagcacct ctttttcttt tttagttcgt tttagttgat
    46021 tccatgtatg atattttcca tcacaatcac tttttcattt tcttcctctt cttttttgat
    46081 tcgttttaac agctgttctg ctgctcgttc cccgatttta tctgttggtg gcgtgatcgt
    46141 gattttcgga aggatactca gatcgttttt atctaaataa tcatagtgac tgaacgcaat
    46201 atccttcccg attctgacac cttcttgatt gaaacattcg atggcaccaa tcgacatatt
    46261 gtaattgctg acaaaaatag cagttgcccc tttttctaat aatgcttttg ccccgtaata
    46321 gccgcttttt ctagaatagt ctcctgtaaa gacctctaat tggatcgatg gatcattttg
    46381 gaccgcttct ttggctcctg ctaaacgttc tcttgctacg tagtctgttt gagtggcagc
    46441 aataaatccg atttttttat gtccttgttc accatgatga gcgatcactt ttgctacact
    46501 ttctcgatca ttcactaata ttgtatcaat acctgctggt ccttctggac aattcaatgc
    46561 gataactgga atactcattt ttgtgagcat ttcaaatcca gaccactctt cttcggataa
    46621 gaagacaatc agcccatcga tcacatgctt catcaggtat tcaatttttt gttcaatttt
    46681 ttctggctga tcggcaaacc cgcttaataa taagctatac ccattttctt cagctactct
    46741 ttcgatccct gagacaactt ccgacccaaa acggctggaa atcccgttta tcaaaagtcc
    46801 aatagagaag cttcgattat tcttcaaccc tttagcaatg atgttttctt tatagtccaa
    46861 cgctgaaatc gcttctgcta tttttttcat gttcttttcc ttcagttgct gaccattcaa
    46921 gtaacgggaa actgttccca ctgaaacacc agccatctta gccacatcac ggatcgatgc
    46981 catacattct cactcctgcc agttacattt gatttctctt tcttgttgtt cgattatgat
    47041 gaccgcattc tttgcatagt gttgcttgtc atcaatatcc atataactaa agtcttcagt
    47101 agagcaactt cctctcacta ttaattatac ttaaacaagt atttgaaccg gttcattttt
    47161 aatatagcat ttttttaaaa gattgcaaca cttttttaaa acgctttctg aaagtggcgg
    47221 attttttgcc tttttaaaat tttattctaa aatttactct atttgtgggt gttttgtgcg
    47281 tttttctctt ccatccgttt gaagttttac attccaatgg tataatttct ttatcctatc
    47341 aaatggggga agtattttga agaaattaat cgtactattt attttgtctg cgggactttt
    47401 gacattgact ggctgtgatt tgtttggcgg ctctgaccaa tcctcggacg aaacaaccga
    47461 ttcttctgtc ttaaaagcgg aaaaagcaga taccaccaac agtgtatata cgctaaaagt
    47521 acgtaaagat atggatttat ccgcgggaca gttgacattt acagataaaa aaatggaatg
    47581 gacacgcacg tatcagacac aaggaactga ttcttctgaa tcatctgaag aaaaaacagt
    47641 gctgacagat atcaagatca ctaccaaaga tgacgaatat attatttcag gaaaagaaaa
    47701 taaaaaagaa acaacagtca tatttacaaa aatcggtaat taccgtttga aggatgagga
    47761 tggcaacatc tacagtctat agtgccgtct gttggatctt cttttgtttc tccccttctt
    47821 ttctgctata gtaaagaagc aagatgattg taaaggagac gaaatggatg atcgatagtc
    47881 ctgattattg tacatgtaat aacaacaaaa atatccatac agaatttaat gaccaaggaa
    47941 gcttgagcat atgcgacaac tgtcacaaac tagtcaaaga ttcatttaaa acatttgaat
    48001 cttatcctgt tcaaaattat gtaaaaagta gaagaagata aacaaatcca agcaaaactc
    48061 actatggctg atttttatga acttcaaact cagcttcttc ctttgatcaa gcttcatcag
    48121 taaaacaaac agtggatcat ttcagtttac aaatcaaaga gtaaagattt atttatttca
    48181 aatatttgaa tataaaataa ccaaacgata gaaaggctta ttctacatca cgtagtaatt
    48241 tttctttccc gtcgtttggt tttttatttt tagcctaatt cttgaccttt tgcatgctgg
    48301 ttatccgtaa aatattttcc agcaggcttt ccttccaatt cagctaattt ttcttggatc
    48361 tgacggatca ccttttttgc atcttctgga ttttctacaa aatcgaattt atccccatcc
    48421 acttgtattt taggacatac atcaaaatct tcataccatt gattatagcg ggtgttcaac
    48481 gtttcataat aggtatataa cgtctcatcg ctttctagct gttcatagtc acgcccacgt
    48541 tttttgatac gttctagcat cgtgtccaaa gaaactttga tatggaccat caaatcaggc
    48601 cgtttttttg gagcaacatc atctaactcg tttagcatcg tatctaataa gctatcgtac
    48661 tgctgtactt cgatatccgt tacccgacct aaatcagcgt ttagatggaa caacaggcta
    48721 tcttcataga tggatcggtc tagcacattg tcatcgttca ctaaagcatt tttcattgct
    48781 aaaaatcgct tgttcaaaaa gaagatttgc aataaaaaag cgtactgctc aggatttgaa
    48841 taaaagagcg gcaatacttc gttatcttcg acattttcat aaaatggttt agaatcaatc
    48901 tcttttgcaa gcatatccgt caaactgctc ttacctgcac caatcgttcc tgaaatcaaa
    48961 atcactgaca tacaaccctt cctcttctat aagtactcgc ctacgtaagt ttcaacaggc
    49021 aatgtttcaa aatcatattc acgtacagct tggtctgatt ttaaatacgt atggagactt
    49081 tgtgtaatca tcaagttcgt aggtaccgtc tcttcttgta caatgatcat cggttttttc
    49141 aacatagttg ccacacctaa ttcgtaagcc gttcctggat cgattgtttg atgctcaaag
    49201 tcaaggatag caacaacaaa ttctgcattt gtcagttctt ccatatcttt ttcgtatact
    49261 tcctgcgccc atccagcgct gaatagttca taattgcttt cttggtgatg acgtggagag
    49321 tagaaacttg tgactgtttt gttttcctcc aacgcttttt cgatacgact tactcgatcg
    49381 atttgctcct ctgaaaaaaa tggtcctgct aaatatattt gactcattat tttgcctcct
    49441 tcaataataa ctgattttta atagatactg ctcgtaattc attgaccagt tccaatggat
    49501 tgttggaaaa gattacttga tccaaccatt ttttatccat aaaatcctct tttgcaaaag
    49561 tttctaataa ttgttgcaag ccgctgtaat aaccgccaat attcaatatt ccgattggct
    49621 tgtcgggcgt cagaccgatt gctatccaag aaagaagttg tgagaattcc tccaatgtac
    49681 ctagacctcc agggaaaacg aggacagcat ctcccttttc aaataataat acctttcttt
    49741 catcgatcgt ttctgtagga ataaatgtag taacatcttt tccaggcaat tcagtttcaa
    49801 aaagcccatt cggatagata cctgtgactt ttccgttatt ttccagtact gttttactaa
    49861 tcactcccat aatcccggag tatgaaccac cgtaaaccag ttcgatttct tcttgtgcca
    49921 tgtattttcc taacgtttgc gcaataaatt tgtaactttc tttattacca aaacgggaac
    49981 cgcaaaaaac agtaagtttc ataaaaaaac actccctaac tcaaaagtct atcttcattc
    50041 tacacgtgtt tttttaaaat aaaagactag attttgtagt tttttagtgg tatataggat
    50101 agacgccact atatatagta tttagaaaaa aatcattttt cagccatttt atagataaaa
    50161 caaaaataaa taacgtgttt tcatcgatat gaaatgactt acttatagaa gatactggga
    50221 tttcgtccta tatgccaata acgtgtatta ttttaaacaa caaatacttg tttcttttat
    50281 taacgaaaaa atcattttgt tctcgtgtta tgtttcactg ataaatgatg tttctctaaa
    50341 taaaaaagcc gacgaactaa acgatcaaaa tttgatcatt tttgttcatc agcttctcct
    50401 aaaaatcttt ttgttaacga taaatagctg gatttgtaaa aatatcactt gaatcgtcac
    50461 tgctggaaga aaattcagtg ataaccgcac ctgttgcgcc tgcctggaac tagtgtttga
    50521 tattgggctc gaacgtgtac tgatctctag catgaagaat aatttctttt tttactgtat
    50581 aataactttc tttccttttt ggaatagtac aggaaagttt ttttgtttcc tttccttcga
    50641 cataaggtat accagaccct ttcgacaacg aaaggttctc atttttcctt catcgtcatt
    50701 tcaaggaggg tgccgatgct ctggacagtt tcgatcgctc ctaaaccaaa atctgtatat
    50761 tcgattcttt ctaactctga ttctttcata ccattcctgt ctcaaggtac atctattgta
    50821 cttttttctt gtaatccatt ctgcctctcc taactaattg tcaaatcatc ccaagatatg
    50881 tcttcttcct ctttttcttc ttggatgatt tctgcattcg gatctactgg ttttttcaat
    50941 aataaaatgg ttattcctgt gacaaaagaa ccagctaaca atgcaccaat gaatgcccat
    51001 ggtctggtca tcgtagcgac tgcaaacatt cccgatgccg gaatacggct atctgcgccc
    51061 caaaacatgc ttaatccgcc acctactgtc ccaccgactc ccgttgctat gacattgcgt
    51121 gccaagtcgt tcaatacgat agggaaacat ccctctgtga tctgacagat ccccatagga
    51181 aaagcggtct tcaatgtatc gatttcttga cgattgagta tatttttacg aatgattttc
    51241 cctaagaaat aagcgatcgt caagccgaat ggggttacca tcgaagctaa aatcaataca
    51301 gtgattggtt catagattcc ttctgaaaat cgtgccacac ccccttatcc agaataaatg
    51361 gtaatccaga aacagccgtg tatctgcggt aataagccta accaaacgaa aaatatgaga
    51421 agctattttt gtttggctgt tcttattact tgatgcaggg cagctttttc acaacctctt
    51481 tttttattta tgtttcataa tcaatatggt aaggatgcag ctccgtatgg tagaccgcct
    51541 ccgaatattc aatcatttcc ccaatttgcg tagacgattt ccgtatccgt ttgatgccga
    51601 tctgtgcgtc tgttttcaga attccttgtt cttcttctgt taaaaatact gccgagaaat
    51661 catcagaaaa tgagtcaata gtatatcctt ttttcgtcag caaacgatac aaagattctt
    51721 tgccaaattc ttctctgctg aattgcatca tttcttgtgg aagataatga acaaaataaa
    51781 tataaggctg attgttcaaa taatataagc gcttaaaaca aacagcttgc tcaccgaaaa
    51841 aggaaaacaa aggatcttcc ttctttatat ggaccaattg caaatccagc gtttctttcc
    51901 ggacttcata tcgggagttc tccaagattt gcgtaaagct tgccgctttg gataatcgat
    51961 tgtaaggacg attgcttaga accgtcgtcc cacgaccact tttctttgcg acatattcat
    52021 ctgatgccag cagttcgatt gcctttcgga cagtgatttt gctgacttga aacagttctt
    52081 ctaattcact ttctgtcggc aacatcgaac cgactggata ttttcctgag aaaatatctt
    52141 ctttaatttt atctgcgact tccaagtata gtacacgtgt ccttttcata acgtcaccct
    52201 ttgctttact attactaaaa gaatagcaca taaaaaataa aaaaagaaca caaatcacat
    52261 gtgagaagtg tgttcttttt cattcagctg ctttaattac aagtttacgc catttgaggc
    52321 aattacttct ttataccaat caaaagattt tttctttgat cgattcagtg tcccatttcc
    52381 ttcatcgtct agatccacat agataaagcc gtaacgttta ctcatttctc ctgttgatgc
    52441 actgacaaga tcgatacatc cccaaggcgt atagcccatc aaatctacac cgtctgctac
    52501 tgcattagcc atcgcttcga tatgacgacg taaataatcg atccgataat catcaataat
    52561 cgtgccatcc tcttccatat gatcaatggc tccaagaccg ttttcaacaa tgaataatgg
    52621 tttctggtaa cgattgtata attcattcaa tgcgattctc agcccttctg gatcgatctg
    52681 ccagccccat tcacttgcct caagaaacgg attttttact cctcccagca agttcccttc
    52741 tgaagttgct gcttgtggat cagtctcatt gattgccgta gacatataat aactaaatcc
    52801 gatgtagtcc actggatatt cttttagcaa ctggaagtct ttttccgttt gctctaattt
    52861 ttccggatcg actccatatt ttttatgggt acgcgctgtg tatgcagggt attctccacg
    52921 tacttggaca tccgtgcaga aaaaattgaa ttcttggttt tggatcattg ttgctgcctg
    52981 attgactgga ttggcatcga tgctgtaagt tgttgcatag ataatcatgc agccgatttg
    53041 gatatccgaa cgcaattcgt ggccgatttt tacggctttg ctacttgcaa cgaattgatt
    53101 atgccatgct tggaaaatat tttggtactc tcctgctcca ttgcttttta ctaatccttg
    53161 acttaaagct ggaaagtgaa aggccgaatt gatttcattg aaagtcatcc aatatttgac
    53221 tttactgctg tatctctcta atactgtctg agcaaatcgt tcatagaagt cgatcagttt
    53281 tcgatttttc cagccaccgt attcttttgc caaatgaaga ggcatttcgt aatgagaaat
    53341 cgtgatcaca ggttcaatat catattttaa gcactcatca atcagctgat cataaaattc
    53401 taatcccgct tcgttaggag taccttcgtc tccatttgga aaaattcgtg tccaggcaat
    53461 cgaaaaacga taacatttga atcccatttc cgcaaacaag gcaagatctt ctttgaaacg
    53521 atcgtaatga tcgattcctc gatgattcgg gtaaatatat ttttcttgat caatcgtcca
    53581 gtcaaattct tcactgccga taatcgcaaa gcgctgctta cctccaggca tagcatctgc
    53641 aacggatagt ccttttccgt ctcgatcata agccccttct aattgatttg gcagcagttg
    53701 ctccacccca taaaaatttt tctgaaaaac gtgattgcat cctgatttcc tccttatttt
    53761 acaattgcca gaacatcttc gtttgcaata atctcttttt ggtctaattc aagtatatcg
    53821 gcaaatgcat cagtattcgt aatgacaact ggagtgatta cgctatagcc agctgctcta
    53881 attgcttcga tgtcaaaacg aatcaataaa tctcctgctt tgacgagatc cccttgtttg
    53941 actaagatct caaagccatt cccatttaat tctactgtat ccatcccgat atgaatcaaa
    54001 atctccgctc cgtcagtcgt tgtgatccct acagcatgtc cagttggaaa aatagtcgtg
    54061 atctctccgt ctgcaggtgc aactagttct cctacttctg gatcaatagc gatacctttt
    54121 cccatcacgc cagaagaaaa aacttggtct ggtacttcac tcaatggcag tactttaccc
    54181 gttaaaggac tttttaaggt atgccgtgct gaagtaatcg gttttcctgc attcgcatgt
    54241 ttgttttcta attgattgtc ttgtgtttgt tcgtcaaatc ctaaaatcaa tgtacctacg
    54301 aatgcaagaa caaaagcaat tcctgtagct attactgcta ctgtcacatt tgactctaca
    54361 ccatccaccg tattaatgaa agtcggaatc gtcaataagc tgaccaaact gctggagaaa
    54421 gcttttactc cggaaaatcc gataatagct ccaccaatac ctccagaaat acaagcggca
    54481 atgaatggtt tcttcaaagg taatgtcaca ccatacacag ttggttcagt gattccaaat
    54541 aacgaagtca ctgtagaaga aatccctaat gctcgaagtt tcgtatcttt tgttcgcaat
    54601 gctacagcta aagcagcccc accttgtgcc aagatggctg gaagaagcat tggcatcaat
    54661 acatcaaacc catattgttc gatattcaaa aacatgatcg gtacgaatcc ccaatgcatg
    54721 ccgaacatca cgaatacttg ccataaactt cccatgatta gacctgctac gattggacta
    54781 aatccataaa tcgagttgaa gagaccgcct aaagcattac cggcaaccgt cccgattggt
    54841 cctaaagcaa gaaatgttaa tggaaccatc actactaaaa cgacaagcgg aactaaaatc
    54901 atttgcagaa attgcggaat cacttttttt acgaatggct ctaatttact ttgaacccat
    54961 acggctagaa tgattggtat gacagaagaa gtataaccag atggtgaaag aatcactggg
    55021 atcccaaaga aactgatagc ttctcccgca ccagcaactg ctgtgatagc tggatacaca
    55081 agtgccatag caattgctac tgctaaaaac tggttggctt tgaacttttt agctgctgta
    55141 aaagcaagca tgacaggtaa gaaggtaaag acaccatctg ctgcagcaaa caatatttga
    55201 taagtaccgc tatcagctgt cagccagccc atcaccgttg ccaatgataa gaatcctttc
    55261 aataccccgg cggctgccat cgcccctaga aaaggtgtaa atatactaga gataatatcg
    55321 atcagacgat tcaaaatatt tttggactct tcttttccat cctccgaaac tgcaagacct
    55381 tcctcatcca cgatcgattg ataaacatct gctacattac tgccaatcac tacttgatac
    55441 tgaccgccac tttgtacgac ttggatcacg tccgggtctt cttttaactt ttgcgtatct
    55501 gctaaagatt catcttttaa tctgaatcgc aaccgcgtag cacaatgtac taaacttttg
    55561 acattctttt gtcctcctac cgcttcccat acacgacgac caactgcttg attatccatt
    55621 gttttttcct tctttctttt ttatttaaaa aataaaaacc cagattgaaa aaaacagtca
    55681 caaacattcg tgaacctatt ttttcaatcc aggttttgcc tgccatacag taacaatcct
    55741 tatgattgga tgcttttttt ctcacttact actctttgaa tatgaatcgt taaataaatc
    55801 tgctctgcga tccccatttc ctgtttttgc gtttgtcgaa gatactcatt gatccgttcg
    55861 acacaatgaa acgcttttgg atactttcct tgaactaatg cgtagagaaa ttcatcttgc
    55921 tcgtcagatc cttccccttt gagataacgc tgggcaaaaa actgcagatg agtaatgata
    55981 cgctgataat tcaaagattc ttcatcgaac acgatgccga agaatttact gatgatactc
    56041 agtatatctc ttaccattgt ggtagcggtc atcgtcactt gcatgtcttc gtttccttgt
    56101 tggctattca caaaatgaaa ggcaataaaa ccggcttcat cctcagaaag ttccacaccc
    56161 gtcttttgtt tcacgaacaa aagagcctct ttcgctgctt gatattcttt cggataaaat
    56221 ttttttgttt caaataataa tggattaggc agttggatat tttcttttgc tcgtgtgatc
    56281 gcataatgaa gatgatcggt caatgtcaga taaatattag gcgaaaattc aacttttagt
    56341 ttatcttcag ccagttcgac tacttgtttg acccattcga tctcctctgc cggaatatca
    56401 gccatcagct gctggaattg gtttttagca aaagaatctc ttaaaacaaa ttctttttgg
    56461 accgcttctg gcgaaatctc gtccccaatc tttttttgaa aagctagtcc ttttcccata
    56521 caaacgactt cttccccgaa tccatttttc gaaatgacga cattgttatt caaaatttta
    56581 tcaattttca ttttgcttcg ccctctcaag aaaaataaaa aaacctagaa ttcgcctact
    56641 gtttcttagc tgaattctgg ttttgcctgc cgtacagtaa caatccattt cttattcaaa
    56701 atcatcatac catcgaattt ttatttatgc aagccttttc atataaaaaa taaaaaaact
    56761 ggaacacaag tctatctaga aaacaaccaa atcagagatg cacctctttg cgtttctctt
    56821 tttttcagca aattggtttt tccttatcga ttcatatcct ttggctgttt ttaatctgta
    56881 acgacttatg tcccagtatc ctaaaatgtt tttgtctcta gattgaaagc agatctcata
    56941 tcatctgtcg catcttttat cgatgtaaca ctttccagtt ctccttgtac aactagtcgc
    57001 gtgacggcgt ccatgtcgcc acaagtaatt agtgtgacaa tttcttttcc ctcgacttca
    57061 ttcagtaatt gggtatctgt aggagcaact ttttctttaa aaaatgtttt gtatgtatac
    57121 acattttcta aatctgtcaa atagatcttg gcacccatct ctaaattttc tagcggtgta
    57181 aacagcaatt cagggttcgt tgcacgatgg cttgctaagg cataatttcc ttttcccatt
    57241 tcttgatctg gagataatgt tcctgctccg taaagtagtg acgtattatc caagcctttg
    57301 aaaaccggta aattgattga aactgaaggg actgcgatac tagcaatcac tggtagatct
    57361 gttcctctca tttgcgacct catgacccct tctgtcgtca ttggttctac cgcatcaaag
    57421 tcaaaactga catctttatc gttattcttt ttcagatctt cttttgttac attcgctata
    57481 gcatatttat ctcctgtctc tctgacaaaa taatccttga tctgttcatt gaaaatcaaa
    57541 gctagtccta ctaataacaa gaggaacaag aggatattga ctagccatct ttttgctttt
    57601 cctttttttt tcattttctc tccctccaga ctcattttac tgtttttctt ttgctctttc
    57661 caacagccga atgacacatt tctacaactc tgtgtatttt tttcgtacac ctgcgctttt
    57721 ttctacagga tatttcaaat tatttttgat caatttttct tcaatgatct catctagatc
    57781 caggtgaagg ttgtctgcca tcatgtatga atagatcaat acatctgcca gttcttcttt
    57841 gattcgttct aattgtgttt cttttacttc ttccggcgtt ttccattgaa aaagttctaa
    57901 caattcagat gcttccaaag aaatagagat agccaaatct ttttcgttat gaaattgtcg
    57961 ccaattacgt tcatctctaa actggttgac tttttccata ctattcattt gcttgccttc
    58021 ttccaatcac ttcttacttg attaatcata ccattttttt acataaaaaa tagatgatgc
    58081 ttttaaaaaa actcctgttt tcttttattt aaaataaatg tcatagaaaa tcaataattt
    58141 aggtagccac catgtgtatg cagtagcgtg tcttcttccc ttgaggcttt ttgtttttcg
    58201 atacgttctt cttcccaaac agacaacgct actagaatct ctcttaatga ttttccttct
    58261 tcggtgagcg agtactctac tttaggcggg acttggttat aggcttttcg atggatactt
    58321 tcatcttgtt ctaactctct taactgctgg gtcagcattt tttttgtgat cgtagggatt
    58381 tgcttgcttc tccacgccta aaataagcga tattgctctt ccctttatct ggtgtaagca
    58441 aagcagttag cattttgacg atcgtgggtt ccgcttttca cttcttatct acttagtatc
    58501 atgaagtata ctttcgtact aatactaagt agcggaagtg tcaactactt tgtttaacta
    58561 catagcaaaa gaaacagaag ggtgacaacg gtcatcacgc ttctgtttct tttgaatatg
    58621 ctgtgttgtt gtctctatct ccgtgtagac tttcctcaaa cttatccttt gacagtaagt
    58681 cagtaggctg atttgctctt tctagatgcc agatacattt ctacaaaaaa atagtgattc
    58741 tgttttgaaa attcgatttt tagttctgca ttttcgtaaa catccaacat ttttttcgca
    58801 ctgtatagac ctaacccgcc atttttttta gaggtttttc ctcttttgac catctccgat
    58861 agcgtcaaat tcgttggatt tgccttgttc gctatactaa aacgtgatcc ctctttttct
    58921 tccagcagat tgatcgtaat cggactctga tcggctacac gatgatcgaa tgcgttagaa
    58981 ataaaaatag ataaacagcg aataaaaaca accagatcaa tcgggattgt tttcacttcg
    59041 acaggaataa ctacttggat cgggattttc tccgattctg ctttttctag aaattttaaa
    59101 aagacacccc tgacagctgg ggatacaata tttgttagct gttgtatttg cggtttttca
    59161 aaaatagctg cagaatgatc ttctaatatt tctaaaaatt ccaacgtttc ttttttattt
    59221 tcctgaagag taatttttaa tgaaaacagt agacttttat aatcatgttt gaattcctct
    59281 agatgatggt aatattcttc atttatttga tacagttgaa gcgattcctg atgttgtctt
    59341 tcttttactt ggaaactgat caacataatg gaaaaaaaca aagaaaaaaa actgatgact
    59401 atcgacaaaa gagatagaag cagataatta gatgagcgga agtgaacact tcccaatacc
    59461 aagtaaatca cgccccagct taataaaata caaatacctc caaaatagtg ttcagttgga
    59521 taagtaaaaa gcaagttgag aaatctcgtc tgtttgtcta tactttttac taatagatag
    59581 aaaataaatg cgataagtag tgcaaaaaaa cttgctgttc caagatgtct ctttcctaaa
    59641 taatccatca gatcaaaaac cagaaaaaag ctgagtccag tgatagcaaa aaaaagatta
    59701 ctgatcaaac agctgataaa caggtttttc gtagtaaaat aaaaaagaaa catcaataat
    59761 aagccaacta ggtaaggaaa gctcacttta gggataggaa gtaaaagtgt caccaccatc
    59821 aaagctatac aaccaaagct gttttttact agcagttgtt tcgaaaagaa taaatagata
    59881 cctaaatcta aaatcaaagt aaaggtcaaa taaagtgtca ctgttgcgac catcttattc
    59941 ctccattgtt ttgtttcaaa atgtacacat atttttttaa taggagcctg catatgaaaa
    60001 agtattttat catagaagat aaccagtttc acagcgaatg gatcatcaga gaattggtca
    60061 atcatcatat cgagaatatc gtgcagctca cttctatctc tcaaatcaat cagttgatcc
    60121 agttgggcac tattgataat cacgatgttt tctttatcga tatcgacttg aagcaatcac
    60181 tcacaggtat tgatgtcgcc gaacaaatac gtaaaaaaaa tgcacatagt aatattgttt
    60241 ttttcaccgc ttatacgaat tttgcagtag agatcatcaa tcggaaaatc cttccgattg
    60301 gatatatcag aaaagacagg aatttatctg aacaaattcg tcaagtgcta gaacagatca
    60361 attggaaaga acagcagatc gtaaatgtcg ataaaatctt tattgatcaa aaaggtggaa
    60421 gtctcgtcct gtatcctcgg gaaattttat atattgaaag tatcaaaggt ttgaaaaacc
    60481 aagttatggt caaaacaact acagaagata aattagtgaa tacttcttta aaacaattga
    60541 agacagagtt agaaaagtat gcttactttt tacttttaaa atcttactgt atcaacttat
    60601 cgcaattgca gaaaattgat tctgctcatt atgtgttaga atttttcaac ggtgactcct
    60661 tactcgttgg tcgaaagatc tttgaaaaga caaaggaacg tttccatgac ttccaaaaaa
    60721 ctgcttcgag ttgatgaata agagctgaga cgtaagcata gcagatttca gggagtgggc
    60781 cttgaatcgg ctccactccc tgaaatcgtt gatatgattt tacgcttcct cttctgtagg
    60841 agcatctgtt aacgaccatg tgaatgttga aacgtagttg tctacagcat aattggatac
    60901 aggaacaaat aattccactg attgtctccc ttgttccaca ttctctccta atgaataaat
    60961 ccatgtgcca atcccttcat ttgctcctgc agttactaga tctactgcct gatctgctgg
    61021 gatttcgatt ccttcagcgc cagttccaga aacggtagat ggcattccac tgctattagt
    61081 tgcgctattt cgaatcacgg cgtttttgaa tttcaccttt gaaccagtta gtttattcgt
    61141 atcagatgtt gtaccaataa attctgttcg tttgactgat aatttccaac cggcataatt
    61201 tccgctttta tccgttactt gtacgaaatt ctgtacttgt gtttgtgctc cagtactgtc
    61261 cgttccgctt tgtaatgcag catatactgt ttttgctgta cctaacttga ttgttccaaa
    61321 atcaagatct ggagcttggt caatcgtcag taatccggat gttccctgat ttggttcgtc
    61381 aggtgtaaaa ggagtatcag gatcatttgg atcaacagga ggaactggaa caacgtcttc
    61441 aggatttgca gcatctggtt tggtcacaga aatcgttcct tctgtagtgg ctgagctctt
    61501 attctcagca aaaacacttt gtgtacataa taatagactt ccagctagaa cggtacttaa
    61561 tgcaattgtt tttttcattt ctttttcctt ctttctttta aaataaataa tccagcaaat
    61621 aagccaataa gaatgactcc aacaagtatc agccatcgcc agtcaaatgt ttctggctga
    61681 ttttttaaat caatatcctt ttcattcaat tggtccgctg catcatcagt tatttcaaaa
    61741 tcggtatgga gattccagcg tttttctttc caagcaccct tcacaaccgc ttgataatgt
    61801 cccggagcta atttttttcc ttctaatggg acaagtaaat caaaatttgt attgggtgct
    61861 acatctattt tttctttttt tattgaaagg taaggctctt ttgtttcttt tttgtagatt
    61921 tcaaccgatg tagtcaagcc gaacatataa gaagcatttg gattttgaag attcgcctta
    61981 atcacatttc ttccatcgct ttgatccgca taagcttttt ttaatttcaa atctggggaa
    62041 gactcttttt cgctatttca aatcaagatt gctttcactt gagaaaactg attgacgaca
    62101 ccaactgtac ttttgttaga aacgttctct tttttttctt gaaacgtgat ccctcctgcg
    62161 accacaccag ccagctcttt ttctggcgct ttcagtgaaa gtgtctcagt gaccgcactt
    62221 tgaggaggaa cagttatttc tcccactgtt tttgctaagt ttttcatatc ataacttaat
    62281 gtttcatcaa atttactttt tgattctcca tattcgatca caccgtttga attcgtcgta
    62341 gcggagtgga tcaatgtctc aactgttact gatttatctg ttcggttaaa gaccgtgtag
    62401 gggatttcct gtgttttccc aggctccatc cttacattta tatatgtttt gttcttatca
    62461 atctgattag ccggtaattc aaaatctacc gcaaaattga attcggaggc atggacaggt
    62521 gtctgccaaa ggatgaatcc aaggagaagg atcataagta aagagcagtt gtttcgatga
    62581 cttttattcc atattcgatt catcaatcat cctccttgat ctttattctg tcggcgcatc
    62641 tgctaatgtc catgttatct ctgctgagta attaccagct atctgatttt ctttggttaa
    62701 gacaagttta aacgcccgac cgttatttaa atattctgaa taatctgctt gcacattcgt
    62761 tgaattttgt cctctgacaa tttccagttc ttcttcattc actaattgat actcctctcc
    62821 tttcttgaaa tataggacat cctgcataga aaatcctgca tcatttgtga aaggctgaga
    62881 taattttatc gttaagatcc acggattttt cgtttctcgc tgatcataaa tccataattt
    62941 ttcctgtata tccatttggc caaatgcttc ttgtgtcaaa ccagttgctt tgatctgacc
    63001 aaaatcgagt ttggaagggg caagaaatga gagaccacct tctactacga tcttcgtagc
    63061 aggtgcaaaa ggcggggcat catgataaac aactggcgta tcacttggat tgtgattccc
    63121 aatttcatcg ttggtacttg gcttgttctg cacaccctta gcagtagctt ctcctgcttg
    63181 gtcgtttaat acaatttcta atacatctcc tgcaaaaagt tgattttctg ggatagaaag
    63241 aacaaattcc cctgtatcat caatagttgt ttctgacaaa acagcttgat tttttttcaa
    63301 tgaaaccaag gctcctgctt cacctgtacc tgtgatcgtg cgagaagctg cattgatcaa
    63361 tgtggtactt aaattagcag gttcaggagg aatcacatcg actgtcgttt tggcagagtc
    63421 cagttctgtc acttcttggc cacctcttac ttttttagca gcaacaactt gcacttgatc
    63481 ctccgttttt aatctctcat cattgggtac ttgaacttcg aacaagccgc cttgttcgcc
    63541 ttctccccat gggttatatc ctgccccttc attttcatct gtttgtcctt gcgtgagagc
    63601 agtaatttct acagatgatt tcgtcgtatc tgcgtaagta atacgtaaag tcacttctac
    63661 ttcgcctgtc caagcatctc ttggctttgc ctcgacacct tcgggaacaa cgacatgccc
    63721 atagattttt ttatctgcat tcgttggcac tcttaaggat tcaatcatcg ggagctgatt
    63781 attggcactg attcgattat aatttttcat tccggcacct gtctgagcaa attcatttgt
    63841 taaacctgca ctagggcttg atacgaaatt gcctaagtcg gtcccattga tcgataagtc
    63901 tgtcatatca gaaaaataat ccgggtcttt ttcaaagtta tcgaatgttg cgcctgtttt
    63961 tcgccataaa gcgatttctg aatgggcgat ctttaaagaa gaatctccat ttccttggaa
    64021 tacccagccg cctatgccat tggtctcgct tccaatccgt ttgttttgga aatcaaaata
    64081 ggtaggagat tctaagtcca cattcaacac atccccactg atgatgccgg tcttatcttc
    64141 ccctgttttc ccgaccatga ctaaataagt tttttcacct gccgtaaaat ttaaatcaat
    64201 atctccagtt gtatctacag ttgctccgga atctgcgcgt atatcgatat ttgatttttc
    64261 tccagtgact gtaaaatcag ctgaacctgg actttctaga aaaccagaag cgtcataaaa
    64321 ttggatcgct tgatttcctt gattgagtct gttcacgtaa gatttgccat cacctgtatt
    64381 tcttaccctc aatgagcctc cattggttac tgtcagcttt tgcgtaccat tctcaaaacg
    64441 aactgcgctg gaacgcccac tctgcttatc gatgtcgata actgatccgg ctccttctac
    64501 gatcaaagaa aggccttttc ctacaaaacg catcccattg ttcccgggtt gatcgtcatc
    64561 tccagccatt ttgaccgaga tttgtccacc acttgataca tggatttgac tagaatctcc
    64621 ttgcaagcga aatccgcccg tagttgctgt attgatggtg attttcccac cattcgatac
    64681 agtgactttc gatcctgaac ctctcatttg gatgattccg cgattgttgt cattggtaat
    64741 attgatcttt gtatttaccg aaaattcgct tcccgaacca ctgacttcaa acatgacatt
    64801 accaggtagc gtatcgactg cctctccttc atcgctaacg ccatctgccc gatcataatc
    64861 aagagagacc tttgaaccgc ttaagatatg gattcctggc gctgtttttc cactaccagt
    64921 ctcatctcct gaatctagaa aaccgtagaa tttccctaga tacggtccat aaagttgact
    64981 accattgatc acattcaatt gatagcttct acaaaaatag gctttacccg gagcggcatt
    65041 cagtccttcc ttcactggtt ggacatccaa cacaccggaa acaccatcta atgtgacagt
    65101 tgcacgagga gcgtaaatca gtcctaattt ggacccatca tacaaatcaa gtgtcccggt
    65161 aaattttatt tctcccgtaa aatgatatct gcttccccaa aaatcggtca tataagcaat
    65221 gataatggca tttccatcga tatttggtgt caatccacct ggatgtgcag ctgttccttt
    65281 aaaattctgg aactcgatcg actgaggtat ctcattgata taaatatggt gtgtgccgaa
    65341 attgtgagcg aatccaccaa aatcaataac gatatctcgt ttgatctcta attccctgtc
    65401 taaagtcaaa ggctgaatca gtcggatctt agttactgat tcgtctgcga gaagctgcgc
    65461 cagttcttct gttgtggcag cttcgctgta cccatccgct gaaacagctc ttgccagtgc
    65521 cgcttcactg ctatcagacg tttgttcagc tacttctgag gtctcacttg tcttattttc
    65581 ttcttgcctt ttattagtag aagcctcact ttgagattct gtagtttcag aagtaatcga
    65641 ttcttctgaa aaagtagtct tgtcaaaact ttgattctct gattgcgttg attcgtttgt
    65701 tttctctaca tcggcaaaaa cggatgtcac catcaagaga gggaagacaa acaaaatcga
    65761 cgcaatcagc ttttttacac attgattcat tctccaccat cctctctgac gttttgacaa
    65821 ttttatttta tcgttttaat ctatttccaa ttgtactttc atatttaaaa tatgtatttt
    65881 tatggaaaaa tttttctatt aggtgtaaat ttcttttttg tacaaaaaaa aggctgtgac
    65941 aaaagtcttg tcacagtccc ttatcccgaa caagcggtgt tctcccgcca actcctcggt
    66001 cacaacctcc attttaacaa taaacctgta aatgtgaggg caatactttg attgtcacag
    66061 gtaatttatc cccttcatct ccatcaacat tcgtcgtcaa ttcttcctca tcagctaatg
    66121 acacaactaa ttgctcacaa gtaaggtagg ctagattttt cgtagattca tctactcctt
    66181 ttaataggtc tggcaatgtt ttaagcgtat cgatcaaaga agaatctttt aaataaacca
    66241 aatggagctt accatcatcc acttttgcat ttggaagtaa tgtttcaaaa ccgccgactg
    66301 agttagttag tcctatcagc aaggtactgc tctcaatttc ttccttcttt ccatcaactt
    66361 ctacgtgaaa cgagtaggat tgtgtggaag ctaactgttt gattcctgac atgaaataag
    66421 ctagttttcc aaatttcgtc ttcttctccg catctacatc attgattgat tcagggatag
    66481 agccaattgc gacgacattc ataaaatagt cgtcatttat ttttccaata tccaatggct
    66541 tgactgattc aatcgaaaaa tgattgatcg cttcctcagg ttctagcggt atccctaatg
    66601 ctcgagccaa gtcattcacc gtacccaatg ggaaaaatcc gaaatttggc cgatgttctt
    66661 gttcagcgat tccactgatt ccttcgttga ctgttccatc tccgcccatg acaaacacac
    66721 tatgataccc ttccgtagcc gcttcgcgag taaagttttt tgcatcccct gctttttttg
    66781 tatgcaagac gaccacttca tcaaacaccg attccaattt tgctatcgct aattgctcaa
    66841 attcctttgc ttgttcaccg cctgaactgg ggtttaccac taataaaact tttttcatca
    66901 ctttctttcc cttctgattg atttctttaa tataacgata gcataaagaa aaaaattagg
    66961 ctgtgatgaa gtactgttca tgaaacattc atttattttt tgacaagata aaacatccat
    67021 actatgaaac tgaggaaaat taaaacaatg atataggaaa taaaagaaaa tgataggcat
    67081 tgtataccta ataaagtaaa aatcttaggt ggcagataaa aacacaaaaa tagcaagaga
    67141 atgtaatagc gtttcttcca ctttttgtta tcagacctta tagcgatcag aggaatacca
    67201 aatgcataac tgaaaaactc ttgatgtact gttaatatgt cacggccttg aataataaat
    67261 gcgacaatcc ccgctataat tagtgtgcct aacaccaaaa cggaaaattg atgaatatat
    67321 tttatataca aatgatccat tgtacttcct gcctttttat ttgtgagaaa aacaaatccg
    67381 tatacgtgaa taacttctag aaataaaaga aatttgcaaa accactccat ttatctactt
    67441 ctacgtgcat tttttgatat tgttttcttt atttaagatt atacagaaaa atatctaggg
    67501 tcgctggatt ctaagtaaaa aaaattttta aaataaaaag aactaaatca agtagtaatt
    67561 aaactactgt tgatttagtt ctaataaaaa gatttagatg aacagtttta ttgatttaaa
    67621 tagttcatat tttttattta taccgcttat ttactacttg ctgtactaga atctgaagtg
    67681 aatgtactca aaacattttc aaagtctttg tctttgattt ttacattagc gtctttcaat
    67741 acttcgccaa ttactttatt ctggaaggta ctgtcactta attttgtgtc tgttgcaatt
    67801 tctttcaatt cttttttgta tttatccatg tcgttgcctt tgttttggtt tttcaccatt
    67861 ttaaccacat aatattccgt agtataagtt gaagcattgg tagaagtgat cacatcagaa
    67921 acttgaccat ctttcaactt aaaggctgct tctttgactt ctgccggaac cgttgtagta
    67981 gtggaatcaa atttcacttt tccgccatct tcttttgtcg tagtatcagt tgacttgtct
    68041 ttggctaatt tagagaaatc gtctccctta tcagcagctt tcttcacatc ttttgcatca
    68101 tcttcacttg ctacttggat aatttgagct tccacttctg gatggaaaga ttcccaagcc
    68161 gtttttaaat cttcatcggt caatttgata tgtttcttta gaccttcttg aaaggctaga
    68221 ctttgtttga tactgtcttt gaatgtttgt tctgtgtaac ctgcagattt caattgtgaa
    68281 tcaaatgaat cacccaattg tttctttgtt tgatcatatt gagaatcgat ttctttggtt
    68341 gatacgtcat ctccatattt atcttcaaat acttgataaa cgatcaactg ttgcacagcg
    68401 gaatctgctc cttgcatttt tttcaagcta tccattactt cttgttcgct gattgtaccg
    68461 cctttataac taacggctgt tttttcaccg gcattcgtac agcctgacaa taagccagtg
    68521 cctagtacga tggcaagtgc taaaaatctt tttttcattt atcaattctc ctacttttcg
    68581 ttttttgcga attgctaaaa acaaaggtgt tcaagatgaa aatcatcaag aacaccttta
    68641 ctaatctgct cgatacgtcc tgaatagata taaggctaca caatccagaa agttcgctca
    68701 gcagaataaa tgtcaagtag cacatcacac cttaatatac tacttctttt gttaccgttt
    68761 ttcaagtcta tcttgcgttc cgtatttaac gtaactaatt cttagaacaa atgttatcga
    68821 ttttgttttt cttcttccgc tgctgggata ttcaaatcag acacttttat gttcgatttt
    68881 ccagacattt ttttgttgtt tttattatga tatattttta tacaactaaa aatgaatata
    68941 ttaaaactac caaaaataac ttttaacgct gtttcattat atgttttttc tattttatat
    69001 aaacttttgg atacttaata ccaaccggtt ggattgtacc aacatgagaa tgtgtcagtt
    69061 ctatttcctg tcgcacttgc ataggcccat actccttttg ttgcatttgc tgatcgaata
    69121 gagtttccaa ctttcgcttt tgcaccgtga cgattataga cacgactata gtggttagaa
    69181 tgacctgttt ttctcgtaca agttgtatca ctataataaa cccatcttcc agagctatga
    69241 ttaattgctg ttctagaaat ttcccggtct actggtaaat ttgacttaat aatattacct
    69301 ttagcatcaa aatgaatctc tcctggcttt aaagatttgt aatagatttc accgtgaact
    69361 gaatttgctt ccacttcaga tgacatagcg tttcctccaa caaaacctat ccctagtgca
    69421 caaataataa ctgctttttt cataaaacga catcctttcc tttttgattt ttaaagcatg
    69481 atcttcttgt ttcctatttt ggtcagatat ggatcatgcg tgactacgat aactaacgta
    69541 ccttgggaag cttgttgcga taagatttca actactttat ccttatttct tttatccaat
    69601 gacccagtcg gttcatcggc taaaatgatt ttgctattct tcaataagac tctcgcacag
    69661 gcgattcttt gcttctcacc accagataat gtgtagatag gcgtacttag cttcttgttt
    69721 atatttactt ttttcaatgc ctcatacttt gctgttcgct tatctgaaac atttaaattc
    69781 gaaaacttca atccaatatc taaattaaaa tcgattgttt catattcaat caacccaaaa
    69841 tcttgaaaaa gaaaagatat ggtatttctt tttatcaagc gcttctctct cccagttaat
    69901 tgtttcgtct cttgatcgaa caaatagaat tgtcctgaac tgaactgatc attcaatcct
    69961 aatatattca ataacgtact tttacctgct ccactttctc caacaacagt cacaagatcc
    70021 ccagcatccg cagtgaaacg atccaaatga agaatcgttt tgtttcgaat gatttttttc
    70081 gcacctttta tcactatcaa cggctccaat tgttaacctc cattcatcag caataactga
    70141 cttttctttt ctgtagacaa aataaaaaca actgaaagaa taaaatcaat gaagctaaag
    70201 aaaaaaatat taaaaagata tttaggctct ctttctataa gaaaaaggaa gattacactt
    70261 gtcgttcccc atttgatgag gttcatcatg aagaaatctt tatacgtttc aaagagcgaa
    70321 taaccacttg aacgaaagat agcgattttc cgacaattgt agacaaaata tatgcataac
    70381 atgacatagc tcaacaagaa gaaagaaaag aaagtgagta aaaatatctg tatcaagtaa
    70441 tgaaatgatc tgcttaacac tttgacagct atctcttcag tctgagtata cggaaatgat
    70501 aactgcaagt tatcacgtaa ctgactttct tcaatcagct caaataacct ttctttcgac
    70561 aaagagccta tcttgatctt taacggtgga tagatttctc cgctgagaat atttctttcc
    70621 aaatacatac tgtttttcaa tgtcagtatt tcaacaaccg gataatgttc gatccagggg
    70681 atagaaggaa taaatgtata aatgggttga ttgtttttta taaatagaaa tttcggttct
    70741 ttttcactga tttcttgata atattctgtt atttgcgcca ggtcattttt taaatctgta
    70801 tgtctatatc gttcagggat caatataaca gggtgcgttt ctttttcggt tatttggatc
    70861 aaccttccag agacatcaat caaaggaaat ttcttaagat aattcagatt tatttttatc
    70921 cctctgccaa actgttcatt ttcttcattc aagtaatctg atacattgac taatatactt
    70981 ccgtttttat tcaaagtttc atataattcc tgatctgctg ctgatgcaaa acgattggaa
    71041 taaataaaat ccacgtggtt tttcccgatg gttactggat aaaatacatg atagtcctca
    71101 ccagcgattt cttttggtgg ttcatgggaa acagctaact gtgaagaaga aaaaacgata
    71161 gttgctaatt cgacattggc aacggtcaaa agaagagaaa acaacaatcc aaatgtaaaa
    71221 agaatgtatt ggatatagtt gaacgcttct cttaacgaag cgattttttc aactaataaa
    71281 agagaaatca aagagatacc atatattccg ctgaacaaga aaacgtacat ctggaacaaa
    71341 taaagaaatt gctccttata atacataaaa ataaaaccac caatgattcc tgcaattatg
    71401 aatgtaataa tgattttctt tttccccaat aaacaccaca atacttgatg taatgaaaag
    71461 tcattattca acaataaact gatttcttta ttcatcaata agaaaaagga tgataacaaa
    71521 aggaataaaa gaatcaatcc aatagttaaa tacactcgat aattatatac gtctgtattt
    71581 gaaataaccg aatacgaata gctattttca aagtcttcaa aaatattagt ggcataatca
    71641 gtgtgaaatg cttgattgaa tcgatcagtg aatagtgaaa tgaaccgttc ataggcttct
    71701 ttttctctcg tcatcaagaa aaattcacct tcaaaatctt ttattttgat cgattcttca
    71761 atggacgtta cgcgaaagga ggtattagaa tagggtacct tctcgtgaga agatttgaca
    71821 tccccttgat aatacaatgt catttcaagc tttggctgta tcttgaagtt ccaagatgta
    71881 tctacttcat tggattcatt tactgttttt ttcatataag aaagctcagt ttctgatgag
    71941 acattttcaa ataccatgat tagctgctca tattcttctt gcccaataaa atgttgtggc
    72001 aatcgaatcg gagtataagt atctgtttga tggaaaaatt catttgtccg atagagtacc
    72061 tgtttattta tactaaccaa ggcaaaacca ctcatgatac cggtcactat gattgtgagt
    72121 aaaactgtga actttttttt gattatcacc actctccctt acttaaatga ttctttgata
    72181 aaatcatacc gtttttcatc aaaaaaacgt atctatcaag tgtataggtc atgtagaata
    72241 aatctatata ctgtagaatc gaaaggagaa gaagatgaac accggagaaa aaatacgaac
    72301 ttaccgcaga aattgtaagc ttacgcaaaa agaattaggc gaaaaaattg gtgtttccga
    72361 taaaacgatt tctagttggg aaaattctcg tacaatgcca gaccttgaaa tgctgtcact
    72421 tttacatcat actttaggtc tgccgattga ttttccttct attgaatcag ctaccgaatc
    72481 aatagaacca acattgccag atgaacctct tgttaacaag aaatcttatt catcaaaaca
    72541 acccttctca ctcaaaaaga cactatcttt ctttctgctc gtcttttgtt tggtctatct
    72601 ttttcttatc aattatcaat acaaaaatga aacgatcgat cgattcaatc cttttttgaa
    72661 accacaaatc ggctatacca cactccctgt aggatacgtg gacaattcca tcgaacgttg
    72721 ggtatgtaat gatatctttg gccatggtac atggctgtct tttcacgctg gagaggtaga
    72781 aaaagagaaa aaccatgctg taatcaaaca caaaggaaaa cacgtcatga acgttcgcct
    72841 gctcacttgg gaagccattc cctcagatta ccaagaaatc ctcagccaag actatgacga
    72901 cgccttaaaa agaagccctc aggagccaga taggcattaa gtaggagatg aacgtagaga
    72961 tagaaaaaat caatgcacat gatcttatcc aaaaaacagg ggtacaaatt aattgaaaaa
    73021 gccttgagat gaaagttcct aaataaaaaa gttcagaatg ataggtattt ctggcttata
    73081 gtataatatt tacgccacag cctcccaatg attctttttg gaatcataat caaggatgga
    73141 cattcacaaa aatattttgc tcattcgatc cgaatagatt aacgaccaat ctagtaagta
    73201 atatagattg gtacgtagtc aagctgcaca taatttagat caattttagt tcttttttat
    73261 atttctatga tctgctcatt tacttttata tgatggctta cagtaacata aataaatatg
    73321 ataatcgctg gtagtttatc caagagtact caataaaata caaaatggat tgttcaagcg
    73381 cctatcataa tgataaaatg aaacaaataa acgatacaag gaggaagaga ctttgagtaa
    73441 tggcagaaca attttttcat tgtaaaaatt gttcagttgt taatctatgg gatttcggta
    73501 gcatgggcat ttttttatag aggaatcaca ccaacctggc cgttttatct cttgttagtg
    73561 ggaatattag taggaaattt tgcccctctt cactatcgta cgaacttttt tccaactgat
    73621 tctggccttt ttcttaaaac aaagacgcga aaattagaaa acattattga aatgacaatt
    73681 acctgtataa tcattttctt agttatattc ctaaacgtct tctaataaga atctcttaaa
    73741 attagcttgt tacagttagt tacctagttt atataacaca gttatcttat cttccttatc
    73801 aacatctttt gccaactctt tgtcttaaat tcgaaacata taagcagagc tacgaacacc
    73861 aagactgttt cggctctgtt ttttgcatca aattcactct tatatagctg ataatctcgg
    73921 ctctttaaaa gaaattattt tcgcttcatt ttattataaa aaggctagga accttgatgt
    73981 tatcaggttc ctagccttta tttaagtatt accccacaga cccttccata tagattttta
    74041 aattacacac tagtttaaac taaaacaaac aaggaaaaac ttaccattta atcaaaaaat
    74101 tagctcatat gaatatatta gaatgtaaat tagactggtc aaaatctggt caaaaaagag
    74161 gacgaataca ggtagtcctc ttttttgtta ttttgatatt ctaaatcatt ttttattgga
    74221 ttattccctt gttcgatata gacccaaatt gatctatcaa taattatcaa actgagtcat
    74281 atagagacaa aaatttcaaa aatgacctgt taaatggtca tcgaatatca tttaaaatca
    74341 atacgacgga gtcagacacg gagtcaaaac aagcccgctg agagtaaaaa atatacagtc
    74401 ccataggtaa agtgccacag gactgtatat ttttttattt gagattacct gaaatgattc
    74461 catttaattt ttatctcatt ttttcctact agttagtatc aaatactctc ctattttctc
    74521 aataaactat tttgggttac atggtatctg aaacagctaa aaccttgtca ttatagcaaa
    74581 aaatcacttt atctccgttt catttcagat acatttcact taagcggatt ccaaaaaaac
    74641 aaaagaaaaa ggaaccccta gttagcctat gctttttatt ggttcctctc aaaacacggt
    74701 catataacaa cggggtg
//
LOCUS       DARBDV010000003        73980 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000003 DARBDV010000000
VERSION     DARBDV010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 73980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.003"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..562
                     /locus_tag="SCM56_000177"
     CDS             <1..562
                     /locus_tag="SCM56_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEG1109009.1"
                     /translation="LKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLYKFVTK
                     HIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGITLTTA
                     HKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNLFLFSE
                     VGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            762..1076
                     /locus_tag="SCM56_000178"
     CDS             762..1076
                     /locus_tag="SCM56_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG1109010.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            1097..1474
                     /locus_tag="SCM56_000179"
     CDS             1097..1474
                     /locus_tag="SCM56_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG1109011.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            1484..2257
                     /locus_tag="SCM56_000180"
     CDS             1484..2257
                     /locus_tag="SCM56_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109012.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            2279..3682
                     /locus_tag="SCM56_000181"
     CDS             2279..3682
                     /locus_tag="SCM56_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEG1109013.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3864..5048
                     /locus_tag="SCM56_000182"
     CDS             3864..5048
                     /locus_tag="SCM56_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEG1109014.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            5045..5335
                     /locus_tag="SCM56_000183"
     CDS             5045..5335
                     /locus_tag="SCM56_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109015.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            5332..5553
                     /locus_tag="SCM56_000184"
     CDS             5332..5553
                     /locus_tag="SCM56_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109016.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5595..6374
                     /locus_tag="SCM56_000185"
     CDS             5595..6374
                     /locus_tag="SCM56_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HEG1109017.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            6441..6935
                     /locus_tag="SCM56_000186"
     CDS             6441..6935
                     /locus_tag="SCM56_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEG1109018.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            6991..7629
                     /locus_tag="SCM56_000187"
     CDS             6991..7629
                     /locus_tag="SCM56_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109019.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7701..8096
                     /locus_tag="SCM56_000188"
     CDS             7701..8096
                     /locus_tag="SCM56_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1109020.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            8080..10533
                     /locus_tag="SCM56_000189"
     CDS             8080..10533
                     /locus_tag="SCM56_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG1109021.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            10530..12557
                     /locus_tag="SCM56_000190"
     CDS             10530..12557
                     /locus_tag="SCM56_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109022.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            12554..13576
                     /locus_tag="SCM56_000191"
     CDS             12554..13576
                     /locus_tag="SCM56_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEG1109023.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13593..14522
                     /locus_tag="SCM56_000192"
     CDS             13593..14522
                     /locus_tag="SCM56_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1109024.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            14767..14883
                     /locus_tag="SCM56_000193"
     CDS             14767..14883
                     /locus_tag="SCM56_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEG1109025.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            14899..15702
                     /locus_tag="SCM56_000194"
     CDS             14899..15702
                     /locus_tag="SCM56_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEG1109026.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            15937..16173
                     /locus_tag="SCM56_000195"
     CDS             15937..16173
                     /locus_tag="SCM56_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109027.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            16180..18132
                     /locus_tag="SCM56_000196"
     CDS             16180..18132
                     /locus_tag="SCM56_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HEG1109028.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(18204..18701)
                     /gene="dfrG"
                     /locus_tag="SCM56_000197"
     CDS             complement(18204..18701)
                     /gene="dfrG"
                     /locus_tag="SCM56_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HEG1109029.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            19063..20103
                     /locus_tag="SCM56_000198"
     CDS             19063..20103
                     /locus_tag="SCM56_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEG1109030.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            20201..20389
                     /locus_tag="SCM56_000199"
     CDS             20201..20389
                     /locus_tag="SCM56_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEG1109031.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(20447..20800)
                     /locus_tag="SCM56_000200"
     CDS             complement(20447..20800)
                     /locus_tag="SCM56_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1109032.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            21331..21753
                     /locus_tag="SCM56_000201"
     CDS             21331..21753
                     /locus_tag="SCM56_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEG1109033.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            21750..21980
                     /locus_tag="SCM56_000202"
     CDS             21750..21980
                     /locus_tag="SCM56_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1109034.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            22478..22678
                     /locus_tag="SCM56_000203"
     CDS             22478..22678
                     /locus_tag="SCM56_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEG1109035.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            22705..23898
                     /locus_tag="SCM56_000204"
     CDS             22705..23898
                     /locus_tag="SCM56_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1109036.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(23966..25531)
                     /gene="guaA"
                     /locus_tag="SCM56_000205"
     CDS             complement(23966..25531)
                     /gene="guaA"
                     /locus_tag="SCM56_000205"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEG1109037.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(25590..26513)
                     /locus_tag="SCM56_000206"
     CDS             complement(25590..26513)
                     /locus_tag="SCM56_000206"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEG1109038.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            26709..27314
                     /locus_tag="SCM56_000207"
     CDS             26709..27314
                     /locus_tag="SCM56_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG1109039.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            27622..28638
                     /locus_tag="SCM56_000208"
     CDS             27622..28638
                     /locus_tag="SCM56_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEG1109040.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            28779..30080
                     /locus_tag="SCM56_000209"
     CDS             28779..30080
                     /locus_tag="SCM56_000209"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEG1109041.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            30126..30788
                     /gene="deoC"
                     /locus_tag="SCM56_000210"
     CDS             30126..30788
                     /gene="deoC"
                     /locus_tag="SCM56_000210"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEG1109042.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            30809..31207
                     /locus_tag="SCM56_000211"
     CDS             30809..31207
                     /locus_tag="SCM56_000211"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEG1109043.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            31273..32355
                     /locus_tag="SCM56_000212"
     CDS             31273..32355
                     /locus_tag="SCM56_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG1109044.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            32567..34129
                     /locus_tag="SCM56_000213"
     CDS             32567..34129
                     /locus_tag="SCM56_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1109045.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            34122..35255
                     /locus_tag="SCM56_000214"
     CDS             34122..35255
                     /locus_tag="SCM56_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1109046.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            35255..36211
                     /locus_tag="SCM56_000215"
     CDS             35255..36211
                     /locus_tag="SCM56_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1109047.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            36310..37476
                     /gene="deoB"
                     /locus_tag="SCM56_000216"
     CDS             36310..37476
                     /gene="deoB"
                     /locus_tag="SCM56_000216"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEG1109048.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            37500..38318
                     /locus_tag="SCM56_000217"
     CDS             37500..38318
                     /locus_tag="SCM56_000217"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEG1109049.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            38335..39042
                     /gene="deoD"
                     /locus_tag="SCM56_000218"
     CDS             38335..39042
                     /gene="deoD"
                     /locus_tag="SCM56_000218"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEG1109050.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(39340..40026)
                     /gene="gpmA"
                     /locus_tag="SCM56_000219"
     CDS             complement(39340..40026)
                     /gene="gpmA"
                     /locus_tag="SCM56_000219"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEG1109051.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(40198..40779)
                     /locus_tag="SCM56_000220"
     CDS             complement(40198..40779)
                     /locus_tag="SCM56_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1109052.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(40912..41595)
                     /gene="rpiA"
                     /locus_tag="SCM56_000221"
     CDS             complement(40912..41595)
                     /gene="rpiA"
                     /locus_tag="SCM56_000221"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEG1109053.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            41914..42327
                     /gene="rpsL"
                     /locus_tag="SCM56_000222"
     CDS             41914..42327
                     /gene="rpsL"
                     /locus_tag="SCM56_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEG1109054.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            42411..42881
                     /gene="rpsG"
                     /locus_tag="SCM56_000223"
     CDS             42411..42881
                     /gene="rpsG"
                     /locus_tag="SCM56_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEG1109055.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            42975..45062
                     /gene="fusA"
                     /locus_tag="SCM56_000224"
     CDS             42975..45062
                     /gene="fusA"
                     /locus_tag="SCM56_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEG1109056.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            45234..46421
                     /gene="tuf"
                     /locus_tag="SCM56_000225"
     CDS             45234..46421
                     /gene="tuf"
                     /locus_tag="SCM56_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEG1109057.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            46828..47136
                     /gene="rpsJ"
                     /locus_tag="SCM56_000226"
     CDS             46828..47136
                     /gene="rpsJ"
                     /locus_tag="SCM56_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEG1109058.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            47167..47796
                     /gene="rplC"
                     /locus_tag="SCM56_000227"
     CDS             47167..47796
                     /gene="rplC"
                     /locus_tag="SCM56_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEG1109059.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            47824..48447
                     /gene="rplD"
                     /locus_tag="SCM56_000228"
     CDS             47824..48447
                     /gene="rplD"
                     /locus_tag="SCM56_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEG1109060.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            48447..48737
                     /gene="rplW"
                     /locus_tag="SCM56_000229"
     CDS             48447..48737
                     /gene="rplW"
                     /locus_tag="SCM56_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEG1109061.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            48776..49609
                     /gene="rplB"
                     /locus_tag="SCM56_000230"
     CDS             48776..49609
                     /gene="rplB"
                     /locus_tag="SCM56_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEG1109062.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            49652..49930
                     /gene="rpsS"
                     /locus_tag="SCM56_000231"
     CDS             49652..49930
                     /gene="rpsS"
                     /locus_tag="SCM56_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEG1109063.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            49953..50300
                     /gene="rplV"
                     /locus_tag="SCM56_000232"
     CDS             49953..50300
                     /gene="rplV"
                     /locus_tag="SCM56_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEG1109064.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            50314..50970
                     /gene="rpsC"
                     /locus_tag="SCM56_000233"
     CDS             50314..50970
                     /gene="rpsC"
                     /locus_tag="SCM56_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEG1109065.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            50973..51407
                     /gene="rplP"
                     /locus_tag="SCM56_000234"
     CDS             50973..51407
                     /gene="rplP"
                     /locus_tag="SCM56_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEG1109066.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            51397..51585
                     /gene="rpmC"
                     /locus_tag="SCM56_000235"
     CDS             51397..51585
                     /gene="rpmC"
                     /locus_tag="SCM56_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEG1109067.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            51610..51876
                     /gene="rpsQ"
                     /locus_tag="SCM56_000236"
     CDS             51610..51876
                     /gene="rpsQ"
                     /locus_tag="SCM56_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEG1109068.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            51934..52302
                     /gene="rplN"
                     /locus_tag="SCM56_000237"
     CDS             51934..52302
                     /gene="rplN"
                     /locus_tag="SCM56_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEG1109069.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            52339..52647
                     /gene="rplX"
                     /locus_tag="SCM56_000238"
     CDS             52339..52647
                     /gene="rplX"
                     /locus_tag="SCM56_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEG1109070.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            52675..53214
                     /gene="rplE"
                     /locus_tag="SCM56_000239"
     CDS             52675..53214
                     /gene="rplE"
                     /locus_tag="SCM56_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEG1109071.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            53233..53418
                     /locus_tag="SCM56_000240"
     CDS             53233..53418
                     /locus_tag="SCM56_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEG1109072.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            53454..53852
                     /gene="rpsH"
                     /locus_tag="SCM56_000241"
     CDS             53454..53852
                     /gene="rpsH"
                     /locus_tag="SCM56_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEG1109073.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            53884..54420
                     /gene="rplF"
                     /locus_tag="SCM56_000242"
     CDS             53884..54420
                     /gene="rplF"
                     /locus_tag="SCM56_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEG1109074.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            54581..54937
                     /gene="rplR"
                     /locus_tag="SCM56_000243"
     CDS             54581..54937
                     /gene="rplR"
                     /locus_tag="SCM56_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEG1109075.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            54958..55458
                     /gene="rpsE"
                     /locus_tag="SCM56_000244"
     CDS             54958..55458
                     /gene="rpsE"
                     /locus_tag="SCM56_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEG1109076.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            55473..55652
                     /gene="rpmD"
                     /locus_tag="SCM56_000245"
     CDS             55473..55652
                     /gene="rpmD"
                     /locus_tag="SCM56_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEG1109077.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            55696..56136
                     /gene="rplO"
                     /locus_tag="SCM56_000246"
     CDS             55696..56136
                     /gene="rplO"
                     /locus_tag="SCM56_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEG1109078.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            56136..57431
                     /gene="secY"
                     /locus_tag="SCM56_000247"
     CDS             56136..57431
                     /gene="secY"
                     /locus_tag="SCM56_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEG1109079.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            57493..58140
                     /locus_tag="SCM56_000248"
     CDS             57493..58140
                     /locus_tag="SCM56_000248"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEG1109080.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            58331..58549
                     /gene="infA"
                     /locus_tag="SCM56_000249"
     CDS             58331..58549
                     /gene="infA"
                     /locus_tag="SCM56_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEG1109081.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            58582..58698
                     /gene="rpmJ"
                     /locus_tag="SCM56_000250"
     CDS             58582..58698
                     /gene="rpmJ"
                     /locus_tag="SCM56_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEG1109082.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            58716..59081
                     /gene="rpsM"
                     /locus_tag="SCM56_000251"
     CDS             58716..59081
                     /gene="rpsM"
                     /locus_tag="SCM56_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEG1109083.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            59109..59498
                     /gene="rpsK"
                     /locus_tag="SCM56_000252"
     CDS             59109..59498
                     /gene="rpsK"
                     /locus_tag="SCM56_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEG1109084.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            59578..60516
                     /locus_tag="SCM56_000253"
     CDS             59578..60516
                     /locus_tag="SCM56_000253"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEG1109085.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            60544..60924
                     /gene="rplQ"
                     /locus_tag="SCM56_000254"
     CDS             60544..60924
                     /gene="rplQ"
                     /locus_tag="SCM56_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEG1109086.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            61196..62035
                     /locus_tag="SCM56_000255"
     CDS             61196..62035
                     /locus_tag="SCM56_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEG1109087.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            62011..62880
                     /locus_tag="SCM56_000256"
     CDS             62011..62880
                     /locus_tag="SCM56_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEG1109088.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            62870..63667
                     /locus_tag="SCM56_000257"
     CDS             62870..63667
                     /locus_tag="SCM56_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEG1109089.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            63870..64616
                     /gene="truA"
                     /locus_tag="SCM56_000258"
     CDS             63870..64616
                     /gene="truA"
                     /locus_tag="SCM56_000258"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEG1109090.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(64949..67618)
                     /locus_tag="SCM56_000259"
     CDS             complement(64949..67618)
                     /locus_tag="SCM56_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEG1109091.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(67856..68044)
                     /locus_tag="SCM56_000260"
     CDS             complement(67856..68044)
                     /locus_tag="SCM56_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEG1109092.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            68314..68676
                     /locus_tag="SCM56_000261"
     CDS             68314..68676
                     /locus_tag="SCM56_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEG1109093.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            68728..70410
                     /locus_tag="SCM56_000262"
     CDS             68728..70410
                     /locus_tag="SCM56_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEG1109094.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            70527..72563
                     /gene="recG"
                     /locus_tag="SCM56_000263"
     CDS             70527..72563
                     /gene="recG"
                     /locus_tag="SCM56_000263"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEG1109095.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            72612..73613
                     /gene="plsX"
                     /locus_tag="SCM56_000264"
     CDS             72612..73613
                     /gene="plsX"
                     /locus_tag="SCM56_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEG1109096.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            73735..73977
                     /gene="acpP"
                     /locus_tag="SCM56_000265"
     CDS             73735..73977
                     /gene="acpP"
                     /locus_tag="SCM56_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEG1109097.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT        24335 a        13158 c        15499 g        20988 t
ORIGIN      
        1 attaaaaaat gaatttggaa tagaagttaa agattatttt attgacttag atttattaca
       61 gctaataaac agtgtgaaaa gaatgtatag tggagacgac aacggtgttg atttatacaa
      121 atttgttacc aaacatatat ttgttaatct taatattgac gagaaaaatt acccgtctat
      181 ttttaagcac tatctatttt ttattaacga agtagaagat cggataaaaa ataattcatt
      241 atctagtagt gtatctagtt ttaagaagat gtttaaatct cgagatggta tcacacttac
      301 tacagcacat aaagttaaag gaaatgagta tgatactgtt atcgctatta atcttctaaa
      361 cgggaaaatt cctcattgga atgaaatttt taatgtttct gataaaggaa ttagttcagc
      421 gagaaaacta ctatacgtaa tttgttcaag agcaaaaaat aatttatttc ttttctcaga
      481 ggtgggttat tttacgagta aaggagactt gctttatccc actcaattat tagataaaat
      541 agaatttgat tataatattt aaaataccta ggtagtcgtt gttttaaata tagtaggaat
      601 cggacgctca gtacggagcg tctatttttt tacccaattt tgaaaggaag tgaacttatg
      661 aaaataaaaa atcaagaatc aaaaggtcgc tctcccccct ttaaaaccat cacacaaata
      721 agattcattc aaacataaaa acagaaagga taggtaaaaa tatggaactt aaatatgtca
      781 ttcccaatat ggaaaaaaca tttggacaat tagaatatgc tggtgaaggg aacattgaac
      841 aacgtcgaat aaatggcaga ccaaccattc tctctcgtag ttacaattta tactcaacga
      901 ttcaacgagc agatgatatt gttgtcattc ttccctccga agctggcgag aaacattttg
      961 aatccgacga aaaagtcaaa ctcatcaacc cacatattac cgcagaaggt tataaaattg
     1021 gcaatcgagg atttaccaat tacattatgc acgcagacga tatggtgaaa gcataagaaa
     1081 ggaaaggtga aaaaatatga gattagcaga aggtattgtg attgaaaaag accagacatt
     1141 tggcacattg aaattttccg ccttgcgtcg tgaggttcgt atccagaatg aagatggtac
     1201 ggtttccgaa gaaattaaag aacgtaccta tgacttaaaa tcaaaaggac aaggacgcat
     1261 gatacaagtc agtatccccg cttctgttcc tttgaaagaa tttgactaca acgcagaagt
     1321 ggaattaatc aatcctgtgg cagataccgt agcgacggct acttttcgag gggcagacgt
     1381 tgattggtac gtgaaagcag acgatcttgt attaaaagga cagacaaaac ctcatcagaa
     1441 atataaaaaa gaagcgtccg aaagtgaaaa ataggtgttg cttatgacaa acacaccatt
     1501 gattttgaaa gaaattccga aggatgaagc catttctttt attcggcaat accattattc
     1561 aaagatactc cctagattgt gtaaatactt tctagggatt ttttcagaag aaaaattatt
     1621 aggcgttgtg gaattaggct ggggtacgca acccctacaa accatacgca aactctttcc
     1681 cgacagttcc cttcaaacca cagattattt agaaattgga aagatgtgtt ttcttccaga
     1741 aatgaatcaa accaattatt ttggcagtca agctctttca gctctaatta agtggttgaa
     1801 agaacatacg gactgtcatt ttttatatac actggcagat ggaattgagg gaaaatgtgg
     1861 ctatgtctat caagcgtcca acttctttta ctgcgggtat ttcaagacaa gtgtctatcg
     1921 tgataagcag tcatgggaaa agatacaccc tcgaagtgct cgccttttat tagaggaaaa
     1981 tgctcgcttt gaacaggtag agaaaaagca ttggctttcc caggcatttt gcgaatacaa
     2041 agggattgag aaaatcaatg gacgcatgtt tcgctatctc tatccgttga caaaagaagc
     2101 gaaaaaactg ttaggacaca ctctctatcg acgtcattat tatccgaaag aaaaaaattt
     2161 acgctttgaa aagcgaatcg cttatcagaa atatgaagcc atttctcaac caacttttga
     2221 taaacaggct cgtatctaca atacacaatt attttgatag gtggaaagga ggatttttat
     2281 gccattgctt catgacagag gagagcgaat caaagccaac gataagcact tggtctatca
     2341 ttttgtacta atttcattgc tggtagtttt tctagtcgtc ctactccctt ttcattggag
     2401 agcgttacaa tcggtgaact ggcaagcagt tcagttagat acatttttct atcaacttca
     2461 tacgccatac ttttggagca gtattctcac tgctcttttt gtatgcggac ttatcagctg
     2521 gctttattat cactatcgta tagattctat aaaacagtta gagcaccgtc aaaagttagc
     2581 tcgtatgatt ttagaaaatc attggtatga agccgaagaa actcaatcag agggattctt
     2641 taaagattta cccagcaaga aaaccaaaca acgaatccgc cattttccta aaatatatta
     2701 tcgaatgaaa gatggactaa tccatattca tgtagaaatc accttaggga aatatcaaga
     2761 tcagcttttg cacctagaaa acaaactaga aacaggattg tattgtgaat tagtggaaaa
     2821 aacgttgaaa gattcttatg tagagtatgt cctactttat gatactattg gcaatcgaat
     2881 tggcattgaa gacgtggtag ctcaagatgg acgtgtcaaa ttgatggaat cggtctattg
     2941 ggagttcgat tctctccctc acatgttaat tgctgggggc actggtagtg gaaaatccta
     3001 ttttattctc acattgattg aatctctcct gcatacaaat gcaaagcttt atattcttga
     3061 ccctaaaaat gctgatttgg cagacttagg ggcggtcatg aataatgtct attaccgtaa
     3121 agaagatatg ttggcatgta tcaatcaatt ttacgaagat atgattgatc gcagtgaaac
     3181 catgaaacaa caccctaact ataaaacagg agaaaattat gcttatcttg gtttgtctgc
     3241 caattttctt atttttgatg aatatgtggc aatgatggat atgctaggtc gtgaaagtac
     3301 caccgttatt cataaactca aacaaattgt aatgttagga cgacaagcag gcttcttttt
     3361 aattcttgcc tgtcaacgtc cagacgcaaa atattttagt gatgggattc gggacaactt
     3421 taacttccgt gtggctttgg gtcgtatgtc tgaaatgggc tttggtatga tgttcggaag
     3481 cgatacacaa aaacaatttt tcttaaaacc catcaaggga agaggttacg tggatacagg
     3541 aaaaagtgtg attagtgagt tttatacacc ccttgtacca aaaaggtacg atttcttagg
     3601 agaaatagga aaagtcattc aaaaaaagca atctgagcct gtaccgcacg aagtgaaagg
     3661 tacaggttca gacttgcttt gattggtgtg gcgttagcca cgccaatcct ttagccccct
     3721 ctatctaaca ggggggcaca aatacaactg aaaacaggca attctcatca accaatgttg
     3781 atggtacaaa ggattgcgga aaatatgaaa ggtgtaaact caagggtcat agtttacatc
     3841 ttttttagat tggaggtttg cgaatggaaa acgaaaaaat ttggattaag gaattgaaag
     3901 aaaagcgact ggcttatggc gtttctcaaa ataaactggc ggttgcttct catattacca
     3961 gaccttacct atcggatatt gaaacaggca aggcagtccc tactcaacag gtgaaagaag
     4021 atttactgaa cgctttggaa cgcttcaatc ccgataaccc attggagatg ttattcgact
     4081 atgtacggat tcgattccct accaatgatg tggctcaaat tatcggtgag gttttacgtt
     4141 tgaatatgga ttatatgcta cacgaagact ttggctatta ttcttatccc gaacactatc
     4201 gtttcggtga tattgttgtc ctagtctctc atgatgtaag taaaggtgtt ttattagaac
     4261 tgaaaggaaa aggctgtcgg caatttgaaa actttctatt ggctcaacat cggagctggt
     4321 atgatttctt tcaagattgc atggaacata aaggtatttt caaacgcttg gatttagcta
     4381 tcaacgataa aacagggatt ttgaatattc cagatttggc aaagaaatgt aaacaggaag
     4441 aatgcatttc cgtctttcga agctttaaaa attatcgcag tggtgaattg gttcaccgtg
     4501 atgaaaaacc atacatggga aacacacttt atattggctc attaaaaagt gaagtctatt
     4561 tctgtatata tgagaaagat tatgagcaat acgtgaaaaa tgatgttccc ttagaagacg
     4621 cagaagtaaa gaatcggttt gaaattcggt taaaaaatga ccgagccacc caagccatga
     4681 aagatttatt ggcacatcaa caggcggaaa aaacagcctt tgaaattatc aatcgctata
     4741 tccgctttgc agataaagac gacactaaac gtcggagtga ttggaaaact aatgaacgtt
     4801 gggaatggtt tatcggtaaa aatcgtggag cattacgcct aaccacacag ccagagcctt
     4861 attcttttga acgaacattg aactggttac atcatcaagt cgcccctaca ttaaaaatcg
     4921 caagcatttt agatgttctc aacggtacaa ccattatctc caccatgatt caggaagcca
     4981 aactgacaga aaaacatgag aaattaattg aacaacaaca tctagccatg gaggatttga
     5041 ttacatgagc cttttaacaa aagaattaaa gaagttaggg tttcagtgtg gcattgagtt
     5101 tcaagcgtat atacaaaata cagggaaata tacgtctctc attattgagg gaaaacggca
     5161 agctggtgat accatctata cctatgattt ttacaaggtg agattttata ataattatac
     5221 caatcgagta accgtctatg gagagcattt aacccctttt caattattaa ggcgtgtaaa
     5281 aagctatatc tattatcgag aaaaatatct taaggaaagg aggacaatca catgaatttt
     5341 gggcagaatt tgtacaactg gttcttggat aatgcacagt cattggtatt gttagcgatt
     5401 gttgtcattg gactgttttt aggttttaaa cgagaatttt caaaactcat tggcttcttg
     5461 attattgctc taatcgccgt cggacttgtg ttcaatgcag ctggcgtgaa agatgtgtta
     5521 ttaaacttat ttaaccgaat cattggtgct taacagatat ttttcatgat aaaataggaa
     5581 gtatatattt tgttatgaaa ggatttatga atatgtttac aaatgatcaa tttaaatctc
     5641 ttttaattaa aacattagaa agaaagagct cttcaaatta ttatgaagga ggtaacgaga
     5701 ttgtttctaa aatgaaaata tcagaggtaa ctttagatga gactgacgat tttacttatt
     5761 ataaaggata taaaaagggc tggaatacgc tttgcacata tcttaaaata agagttcctt
     5821 ttgatgattt agactttttt gaaagtcata aagatttgat tacccagaca gcaagctcca
     5881 tatatgataa acaaggtgat aatgtattag tggatactat tttagtacca cttccagaga
     5941 attacgaagt aattaacttt agtcagttga aaattagtga tgtggtttca caagctattg
     6001 aagacgctga gtcttttatg agtaatggtg aatatcaacg tgcctttgac agagttcaca
     6061 ctgcttttca tggatattta attgaaatac taaaaaaata tgaaattaca gttcctagag
     6121 acgaaaatct atctaaatta tatagtagga tacaacaatt aattgaaaaa gaaattcaac
     6181 ctactgaact tgctgatata gtgaagacaa cgataaggtc atcaaatgga atgattagct
     6241 ctcttaatga agcaagaaat aggcattcac ttgcacaccc taatacaaat attattggaa
     6301 agagagaagc aaaactaata ataggtattt caagtacagt tacagattat attagcggat
     6361 accttgataa gtagcgtcat taactgattg ctactttttt tatgactatt tttataaaaa
     6421 ggatgtgata agaattggaa atgaaagtct atgtggcaaa tttaggacgg tataatgaag
     6481 gtgaattagt cggagcatgg tttactccac caattgatga ggaagaaatg gcagaaagaa
     6541 tcggtttaaa tgaggactac gaagaatatg caatacatga ttttgagctt ccttttgatg
     6601 tggacgaata cacgccgatt agtgaaatca accgtctatg tgaagccatt caagaaatcg
     6661 aaggtacacc tatttataat gaactcaaag aaattcaagg catgtggttt agtagcttgg
     6721 aagaattact ggaaaacaaa gaggatattc attgttatag tgattgtgat tcgatggaag
     6781 atgtcgcccg ttattacgtg gaagaaacag gacagcttgg tgaagtccct tccaatttac
     6841 aaaattatat tgactaccaa tctttgggac gtgatatgga aatagagggt aattacctag
     6901 ttacctctca tggggtattt gaatattgtc agtaagttta acaatccgtg ttataattaa
     6961 attaataaat agcacggaga ggtataaatt atggtaaatg tatttgaaaa tcttaaacta
     7021 ttaaaactag acatgaaaga taaaggctgg gtgatagatt ccttttactt tcgttacaaa
     7081 cagcaaaatt atattgtact tgttaagcta tttgaaaaag aggaaaaagt tccagaatat
     7141 gctctgttaa aactagaatt tttaaaggaa aacgattttt cagatatgct agctgtctat
     7201 gctaattcgg taaaattata tacagacacg aaaacaataa gagaatattt tggtatcgaa
     7261 tacagtagta acttgggaga tgttttattt caatttagcc aaactcttgc tcgatttatt
     7321 ccgacagaag ttagtgagaa gaaaaatgaa gaccaaaaag aagccatgtg tttctcatta
     7381 agtcaaagtg attcagaaga tcccagaaaa aaatattgtt tttcagttag aagaaatcct
     7441 ttaaagggaa atggtgagct gggaaaacga agtcctttta atgacaataa aacgagattg
     7501 tatagaccca gcttatatga acgattaggg tcagatacca atttaagttt cagatattca
     7561 atgaatccag atgatgaaga aactgatgaa ggtattattg caaaatggac aaaaaataaa
     7621 attgaatagt taaagcattg aaagagcaat cacctatttt gattgttctt tttctatttt
     7681 tacggaaagg acgtgaagac atgaaaaaaa taagaagtta caccagtatt tggtcagtag
     7741 aaaaagtgat ttatgcgatt aatgatttac aactcccttt cccaattacc tttacgcaaa
     7801 tgacatggtt tatcgtttct ttgtttgtcg tcatactctt tggcagttta ccaccacttt
     7861 ctctgattga cggagcacta ctcaaatact tagggattcc cattgttctc actgggttca
     7921 tgtgccaaaa gacctttgac ggaaagaaac cctatggatt tctaaagtct atcattacct
     7981 atgctctacg ccctaaagta acctacgctg gcaaacaaat acgattaaaa aaagaaacaa
     8041 tgaatgaatc gattacgatt gtaaggagtg aacaactaga tggcatatcc gattaaatat
     8101 atggaagata atctcgtatt taatcatgat ggcgaagtat tcgcttatta tgaattaatt
     8161 ccctataatt acagtttttt aagttccgat gaaaaaattc aagttcatga taatttctgt
     8221 caattgattg cccaaaatcg tgatggcaag attcatgctc tgcaactagc aaccgaatca
     8281 agtattcgag ccacacagga acaatcgaaa aaagaaatca ctggacgatt aaaagagata
     8341 gcttacgaaa aaatagatga acagaccgaa gcgttaattt ctatgattgg tgacaatcaa
     8401 gtagactatc gcttttttat tggttttaaa ctctacctca atgaacagga agtatccgta
     8461 aaaaatgttg ggaaagaaat tcaaaaagca tttgcggaat tctttcatgg tgtgaatcac
     8521 aaattaatgg gcgattttgt ttctatgcct atggcggaaa tcgaacgctt ttctaagatg
     8581 gaacagttgt tagaaagtaa attatccaga cgctttcaga ttcgtaaatt ggagaaagat
     8641 gattttggtt atattattga acatctttac gggcaaacag gtgtcccttt tgatgactac
     8701 gactaccatt taccgaaacg atacgaagat aaaaaagtgt tggtaaagaa atacgatatt
     8761 ctcaaaccca ctcgctgttt aatcgaagaa aatcaacgct atttacggat tgaacaggaa
     8821 gataccacta cttatgtggc gtattttaca attaatagta ttgttggtga attggatttc
     8881 cctaatagtg aaatttttta ttaccagcaa cagcagttta ctttccccgt tgataccagt
     8941 atgaatgttg aaattgtcgc caataaaaaa gccttatcta ccgttcgcaa taaaaagaaa
     9001 gaactgaaag atttagatag ccacgcatgg gaaagcgaca acgaaaccag tactaatgtg
     9061 gtggacgctt tagaatctgt cgatgaatta gaaagtaatt tagaccaaac caaagaatct
     9121 atgtacaaac taagctatgt ggtacgggta tcagcaaaga atttggacga gctgaaacaa
     9181 cgttgtgatg aagtaaaaga tttttatgat gatctgtcta tcaaattagt acgacctttt
     9241 ggcgatatgc taggcttgca taatgaattt attccctcaa gcaagcgata tatcaatgac
     9301 tatattcaat atgtcacgtc ggattttttg gctggtcttg gtttcggtgc tacacaaatg
     9361 attggtgaaa attctggtat ttacgtcggt tacaatctcg atacaggcag aaatatttat
     9421 ctccaaccta gccttgcaag tcaaggaatt aaaggctctg taacaaatgc attagcgtcc
     9481 gcttttattg gttcattagg tggcggaaaa tcattctcta acaatatgct ggtctattat
     9541 tcagtactct ttggcggaca ggcaaccatt gttgacccaa aagctgaacg tggtgaatgg
     9601 aaagaaacct tacctgatat tgcccatgaa atcaatattg ttaatctcac gagtgagaac
     9661 gacaataaag ggttacttga cccttatgtg attttaaaac tcccgaaaga ttcagaaagc
     9721 cttgccattg atattctcac gttcttaaca ggtatttcca gccgagatag cgagaagttt
     9781 cctgtcctgc gtaaagctat tcggagtgta actcaaagca aacaacgagg gttgttattg
     9841 gtgattgatg aattgcgaaa agaagatacg cccatcagca atagtattgc tgaccatatc
     9901 gaaagctttg tcgattatga ttttgcccat ctgttattca gtgatggcag tgtcacccaa
     9961 tccattagct tggaaaaaca actcaatatt attcaagtgg cagatttggt cttacccgat
    10021 tccaatagct cttttgaaga atacactacc atggaattat taagtgtggc aatgctgatt
    10081 gtcattagta cctttgcttt agactttatc cactctgacc gttccatttt taaaattgtt
    10141 gaccttgatg aggcttggag ttttttacaa gtggcacaag gaaaagctct atctatgcga
    10201 ttggttcgag caggacgtgc catgaatgca ggggtctatt tcgttactca aaacgcggac
    10261 gatttactag atgaaaagat gaagaataat attggcttga aatttgcctt tcgttccact
    10321 gacctagtgg aaatcaaaaa gaccttagaa ttttttggtg tggacaagga agatgaaaac
    10381 aatcagaaac gcttacgtga attagaaaat ggacaatgtt tagtttcaga catctatggt
    10441 cgtgtagggg ttatgcagtt tcacccgatt tttgaagagt tgcttcatgc ctttgatacc
    10501 agaccgcctg tgcgaaatga ggtggaagaa tgacacaaaa acaaaagaaa tttcttcact
    10561 atagttggat aacgctcctt tgtgtgggcg ttattttact tttattaggc acgctgggca
    10621 atgcagtaca agcgacaggc ttagttgatg aaaccattga tacaagcaat gaatactcca
    10681 agtatggttt gaatcaatat cagcttgatt attatgtgga caatagctgg ggctggcttc
    10741 catggaattg gagtgatggc ataggacaat cggtgatgta tggattgtat gccattacta
    10801 attttatttg gacaatttct ctttatctat cgaacgccac aggttatttg gtgaaagaag
    10861 cctatgcctt agatttcatt agtcaaacat cccaagcaat tggcgaaaac atgcagacga
    10921 ttgcaggcat tacaccgaat ggcttttcca gttcagggtt ttatgtcggt ttcctccttc
    10981 tttttattct cattgttgga atctatgttg cctatacagg attggttaag cgagaaacga
    11041 cgaaagtgat acgagctgtt ttaaactttg tcgtggtgtt tattctatcg gcttccttta
    11101 ttgcgtatgc acccgattat attgcgaaaa tcaatgattt ttccaaagat gtcagtaccg
    11161 ccagtctgaa tattgggaca aaaattgttc ttcctaacac ggatagtcaa gggaaagaca
    11221 gtgtggacat gatacgtgac agcttattct ctgttcaagt aaagcaacca tggctattgc
    11281 ttcaatatgg ctccactgat gtagaagcgt taggggaaga acgtgtggaa agtttattgt
    11341 ctaccagtcc agatacaaac aatggtgaag atagagaaaa tgtagtgatt gaagaaattg
    11401 aagatcatga caatacctat ttaaccctac cgaagacgat ttccagatta ggaacagtct
    11461 tctttttgtt tgtattcaat attggtatct cgatttttgt tttccttcta acaggaatca
    11521 tgattttttc gcaagtgcta tttattattt tcgctatgtt tttgccaatc agtttcctat
    11581 taagtatgtt gccgagtttt gatggcatgg gcaggcatgc cattaccaag ttatttaatg
    11641 tgattatgac aagggctggt attaccttga ttattaccgt tgcctttagt atttccacta
    11701 tgctttattc gctgtcagca agttctccct ttttcatgat tatgttttta caaattgtga
    11761 cctttgcagg gatttatttt aaactgggcg atttaatgag cctattcact ttacaaagca
    11821 acgactctca aaacatgggc agacaagtgt ttcgcagacc tcgtcaaatg atgaatcgac
    11881 aaaccagaag atttcaacgc agtatgagta agatttttcg tggaaaagca gggtcaacga
    11941 agcaaaagac aatgacaaca aagcagaatc aaaccaataa agctaaccat actcgtcaaa
    12001 atgaacggac tacaccaaag aaagaaacag cactttccaa tgtgaaaaag caacaaggcg
    12061 gtatcgggca acgattaggt gaaaagacag cccatgtatt ggacacgaaa aatcgtctag
    12121 tcaataaaag aaagcaggca aaacaacaga tacaggcgac accaacccat gtcaaatatg
    12181 cccttcatca aggaaaagag aatgtcactc ataatgtaac agactttaaa caatctatgg
    12241 cagataccaa agctcaacgc aaacaacagc gtatgaacca gcaggagcaa aaacgaaaga
    12301 acattgctca aaagcgtttg gagctggata aagcaaaaga aaaaagacga aaagaaaacc
    12361 catcatctcc tgttcagaat aaccagcagc atgttcgcaa gcgtcagcaa accgtcacgc
    12421 ctgttttgtc acaaaacact aaacgtgaaa atcatcagcg accattgaaa aaagagcaaa
    12481 aacaaaagac accaccgaag caattcaatc agaggaagct aaataaaaga acggataagc
    12541 gaggacaaca aacatgaagc taaaaataac agtgatttgt agtgtggtct tctctctctt
    12601 cttttttctc ttattattcc tagtcatttt ctttgcagat gatacggaca gtggcgaaaa
    12661 caacaaggat tcttctattc cacaaggcgg tgtgaccgtt tcaccagaag tgttggcaca
    12721 ccgtccgttg attgaaaaat atggtaagga atatgggatt gaagactatg tttcttatat
    12781 cttagccatt atgcaagtgg aatcgggcgg tacagcagaa gatgttatgc aaagttccga
    12841 atctttgggt ttaccgccca acagtttgag taccgaagag tccatcaaac aaggggttaa
    12901 atatttcagt gaattattaa ccagtgcaga acaacaagga gtggatatag attctgttat
    12961 ccaatcttat aattatggcg gtggcttttt aaattatgtg aggagtcatg ggaaaaaata
    13021 tacctatgaa ttagccgaac agttttctaa agagaagtcg ggtggtcaaa aagcagacta
    13081 tcccaatcct atagccatac ctgtgaatgg tggttggcga tataactatg gaaatcagtt
    13141 ttatgttcag ttggtttcac aatacctaac cgacacttcc ccaacagaat ttgatgatga
    13201 aaccgtgcaa gtcattatgg acgaagcctt gaaatacgaa gggtttccct atgtctttgg
    13261 aggggcgtcg cctaccacct cttttgattg tagtggctta atacaatggg tgtatgacaa
    13321 agctggcatt tctctccctc gtgtcgccca agatcagtat gacgctacgc aagaaatctc
    13381 tatggaagaa gcccaagcag gcgaccttat cttctttcat tcaacctata atgcaggcac
    13441 atatgtgact catgtggcga tatatttaga gggaaatcgc ttttatcatg caggcgaccc
    13501 aatcggttat ggcgacttaa gcagtcgtta ttggcaagac catctgattg gggctagacg
    13561 tgttattcat aactaagaaa ggaatgaatc aaatgattat aaaaatcgag cgaaaagaaa
    13621 agaaggagaa accaaagaaa tctaaaaaac cgagcaagca aagaaaagtt ccgatgatta
    13681 aagttggcac gcataagaaa ttaacctttg ttttatgggt tttattgatt ggtagtgtcg
    13741 gatttggaat ctataaaaat tttactgcca ttgacactca tactgtccgt gaaacagaaa
    13801 tcattaaaca gcagattgtc gataccaacc aagtagaaag ttttgtgaaa tcctttgcga
    13861 aagactactt ttcgtggcaa caatcccaag aagccattga taaacgaaat gaaaaattga
    13921 cccattattt gacggaagaa ttgcaggtat taaatgaaga aatgattcgt aaggatattc
    13981 ctactagttc tagtgtgaac gatatacagg tgtggcaggt ttctcaagtg aatgagaaca
    14041 cctttgaggt actgttttct gttgaacaag ttattaccga agacaaagac aaggaaacta
    14101 tatcgtctag ctttcatgtg gttgtccata tagatgaatc agataatatg gttattatca
    14161 aaaatccgac aatgagtaag aaacctcaaa aatcggacta ccaacccaaa cagctagaaa
    14221 gtgaccatac agtagataca gaaacgatgg acgaaatcac tagcttttta gaaacgttct
    14281 tccagctcta tcctaccgcc acagaaaaag aattgactta ctatgtaagt aatcatgtgt
    14341 tacctatgat taacaaggaa tatgttttcg aggaattggt gaatcctatc tttaccagaa
    14401 aagataacca agtaatcgtg aatgtcgctg tgaaatattt agatcaagaa acaaaggcaa
    14461 cccaaatctc gcaatttgag ttgatattag aaaaacaaga taattggaag attgtaaaat
    14521 aacaaatatt ggtacatgat tacagatact ttgtaatcat gtactctttt tgataaaaaa
    14581 ttggagattc ctttacaaat atgctcttac gtgctattat ttaagtgact atttaaaagg
    14641 agttaataaa tatgcggcaa ggtattctta aataaactgt caatttgata gcgggaacaa
    14701 ataattagat gtcctttttt aggagggctt agttttttgt acccagttta agaatacctt
    14761 tatcatgtga ttctaaagta tccagagaat atctgtatgc tttgtatacc tatggttatg
    14821 cataaaaatc ccagtgataa aagtatttat cactgggatt tttatgccct tttgggtttt
    14881 tgaatggagg aaaatcacat gaaaattatt aatattggag ttttagctca tgttgatgca
    14941 ggaaaaacta ccttaacaga aagcttatta tataacagtg gagcgattac agaattagga
    15001 agcgtggaca aaggtacaac gaggacggat aatacgcttt tagaacgtca gagaggaatt
    15061 acaattcaga caggaataac ctcttttcag tgggaaaata cgaaggtgaa catcatagac
    15121 acgccaggac atatggattt cttagcagaa gtatatcgtt cattatcagt tttagatggg
    15181 gcaattctac tgatttctgc aaaagatggc gtacaagcac aaactcgtat attatttcat
    15241 gcacttagga aaatggggat tcccacaatc ttttttatca ataagattga ccaaaatgga
    15301 attgatttat caacggttta tcaggatatt aaagagaaac tttctgccga aattgtaatc
    15361 aaacagaagg tagaactgta tcctaatatg tgtgtgacga actttaccga atctgaacaa
    15421 tgggatacgg taatagaggg aaacgatgac cttttagaga aatatatgtc cggtaaatca
    15481 ttagaagcat tggaactcga acaagaggaa agcataagat ttcagaattg ttctctgttc
    15541 cctctttatc atggaagtgc aaaaagtaat atagggattg ataaccttat agaagttatt
    15601 actaataaat tttattcatc aacacatcga ggtccgtctg aactttgcgg aaatgttttc
    15661 aaaattgaat atacaaaaaa aagacaacgt cttgcatatt aagtagttca gttttggagt
    15721 acaaaaagtg aaaatgtaaa cctaaatgag tatcaaattg caactgaatt tgatactcgt
    15781 tttttattgt taaatcaacg tttgttgtta atttacacca caaaatgtaa gttaaaatgt
    15841 atataaaaat gcgaacgtga aataattatt gtaggaatgc aaattggtgt gttaaaatgg
    15901 ttacaattta ctaagcaaaa aggggtgcat tttcctatgc aatttcttga gccaaaaaat
    15961 aagaacgcta agtctgtgga ttgggaaata tcagagcaag taagggtgat agttaagcaa
    16021 tacgctgaat atgcagagcg tactgaaagc gaagcagtgg atgaattctt attgaacatt
    16081 ttagacgata agaagtttat tgaatggatt gccaacaagc gaagtaataa gcggattgtt
    16141 gaaaaaatgg gtatcaaaga tagagtgggt tgatagacaa tgaccaagct taaaagatta
    16201 gccacgaagg aagatgaaat tgtagatgtt aaaattccga tttcaaacga agaattaaaa
    16261 gatagggcta aacaatatga tttattaact ccaaaacgat ttgcaacaag gtataacaaa
    16321 atgttgttct tgccaacgtc ttttaaatgg aacggttctg aatatcccat tcaatataat
    16381 tactgcatca atccgttttg ttgtaatttt gggaaagaac agcacaaatt caaagatgta
    16441 aaagggaaac caagtcgata taaaatgact ggtagttcca aagataaagg tcataaaggt
    16501 atgtactgca atgacaatcc tattggcaga ggtgtatcgc aaaactgtac agtaacccct
    16561 ttatctaatt ggtctgttgt tgaagaaatt aaacgcctta tagaaatcaa tagcatccaa
    16621 gatgttgagc cagattatca atttcataaa gaaggctgct cggaagaaga atcaacccca
    16681 ttcaatgagc cgaagcaatt ttacaaaagg gggaaaagta ggggcaaatc tcaacgctat
    16741 caatgtaagg cgtgtaagaa gttcactaat gttctaccaa aaagagaaga aaccactact
    16801 tatcaccaac aaaagaacac cattcttcca atgttcgcta agatggttgt tggaagggtt
    16861 tcagtcagtc gtacttgtga tattttagga attggagttg gaacctacta tcataagctg
    16921 gagtggctgt ataggcgttg tttggagttt ttagaacgat atgagacaca accccttcaa
    16981 acaaagaaat ttaacgagat gtggcttaat acggacaaga tgcactacta cctaaataac
    17041 gtccgtaaga aaggacaagg ttcaaagaaa tatacggggt ttgaagacct taatatgcaa
    17101 acgtatatag ttgtatctgc tgaggtctta tcaagatatg tgttccgttc tgatgttgcc
    17161 tatgattgga atatttcaat ggatgaatta aatgaagata caagaaaatt caaagaagac
    17221 catctcaata cttttagtcg taaaaatgat aggttagatt ggtcttatta tccacaagaa
    17281 ccatccgcta acgatagcga aaataggaat gcttatcttc acgaattagg gaaaattact
    17341 aataggagta gatttgtcga tggtttaaat gttgatgctc cttatactac aactgctcac
    17401 tattggttaa ttaaacaaat ggttaatgcc gacgaatggc gaatgattag tgatgatgac
    17461 ttctctatta gaaacgcctt ttatagagta ttcacgaaag aattaaggtt gagcgatgca
    17521 catcatttta tttgccaagt taacaagacg aaaagcagaa aacaatgctt aaaagaattc
    17581 ggacaagcaa aagcagaact gctcgattgg ggagatatca gaggattcaa aacaaagttt
    17641 ctacggactt tggcatctca ttatctaact gaattactaa caagtcatca attccacgaa
    17701 gaggctatta gtaaagatgg agaaagatat cgcaaatatg ccgataaccc tatcaaacat
    17761 ccattagcca ctaaggataa aggattttat tcagttgatt gtagaacgga tttatctgct
    17821 ctggaaccaa acgaaatcgc aaagatgcta ttaaatgtta atgaccattc gaccaatagc
    17881 tttattcaac agataagacg ttatatatca tctcttgaaa gaccattaac aacagctcgt
    17941 ggtgataaga agagctatat ttacgctaac ttcaatccga agtatgctca atttgcgatt
    18001 acaattctaa gaacttacta caacttttgt cgccctttta aatcagcgga taaaaaggtt
    18061 ttgacacctg ctcaaagatt agggattaca gataaacaat ttgattggaa agatataatt
    18121 tatttcaaat gacaaaaaga gcttggagta atcccaagct cttgtgcttt atctttactg
    18181 ttcttcttca attaacgcac ccgttaactc aataagtttt ttctttcata tacatgaaaa
    18241 tagtagttat acggattttt atcattcttt atcccttttt gggcaaatac ctcattccat
    18301 tcctcataat tcacttctgg aaaaaaagta tctccttcga attcatgatg tatttttgtg
    18361 atgtacattt tctcaacata agggaaaaac aaattataaa tctgttctcc tccgaaaata
    18421 aaaatttctt cttcgttttt acataactca aaaacatctt ctattgaatg aacaatttca
    18481 caaccattaa aggtaaaccc cttatctctc gtcagaataa tatttcttct gtcaggtaag
    18541 gctcttccga ttgattcaag gttcttccta cctaatatta tcggatgtcc ctttgtagta
    18601 tttttaacat attcccagtc cttgggaatc ctccaaggaa tgtcattctc tttgccaatc
    18661 actctattct tatccatcgc agcaatcaaa gaaactttca tttataacac agccttttta
    18721 actaatttac catctaattt cttatttcta tcaaaaaacc ttttctcatt atcctgcccg
    18781 ttagttgaat aagcaatttt ttctataagt tatattatca taataccaca aaatcacaaa
    18841 ttttcattca attatcattt tcaattgcat tttataaatc attttgtatg taatttggta
    18901 tgtaagttaa aatgaaaaaa gtagtccatt ttgaagtaca ttttgtcatt ttgtactcca
    18961 aaactgaact acttacttgc atatatacgc ctttatagtg gagtactaca tttacgagat
    19021 tcggttagag tatcagaaaa agaaaaaata aaagttacag aaatgtatac ttcaataaat
    19081 ggtgaattat gtaagattga tagagcttat tctggagaaa ttgttatttt gcaaaatgag
    19141 tttttgaagt taaatagtgt tcttggagat acaaaactat tgccacagag aaaaaagatt
    19201 gaaaatccgc accctctact acaaacaact gttgaaccga gtaaacctga acagagagaa
    19261 atgttgcttg atgccctttt ggaaatctca gatagtgatc cgcttctacg atattacgtg
    19321 gattctacga cacatgaaat tatactttct ttcttaggga aagtacaaat ggaagtgatt
    19381 agtgcactgt tgcaagaaaa gtatcatgtg gagatagaac taaaagagcc tacagtcatt
    19441 tatatggaga gaccgttaaa aaatgcagaa tataccattc acatcgaagt gccgccaaat
    19501 cctttctggg cttccattgg tttatctgta tcaccgcttc cgttgggaag tggaatgcag
    19561 tatgagagct cggtttctct tggatactta aatcaatcat ttcaaaatgc agttatggaa
    19621 gggatacgct atggttgcga acaaggatta tatggttgga atgtgacgga ttgtaaaatc
    19681 tgttttaagt acggtttata ctatagccct gttagtactc cagcagattt tcggatgctt
    19741 actcctattg tactggagca agcctttaga aaagctggaa cagaattgtt agagccatat
    19801 cttagtttta aagtttatgc accacaggaa tatctttcac gggcatataa cgatgctccc
    19861 aaatattgtg caaatatcgt aaatactcaa ctgaaaaata atgaggtcat tattattgga
    19921 gaaattcctg ctcgatgtat tcaagattat cgcaatgatt taactttttt tacaaatggg
    19981 cttagtgttt gtttagcaga gctaaaagga tatcaggtta ccactggcga acctgtttgc
    20041 cagacccgtc gtctaaatag tcggatagat aaagtaagat atatgttcaa taaaataact
    20101 tagtgcgttt tatgttgtta tataaatatg gtttcttatt aaataagatg aaatattctt
    20161 taatatagat ttgaattaaa gtggaaagga ggagattgtt attataaact acaagtggat
    20221 attgtgtcct atttgtggaa ataaaacaag actacgaata cgagtggata ctatacttaa
    20281 aaatttccct ttatactgcc ccaaatgtaa gaacgaaact ttaattaatg ttcaaaaaat
    20341 gaatataata acaatcaaag agccagacgc caagacgcag agccgataat ttgagaaatg
    20401 aaactctcat cttatcggct ctttttgttt atctgaattt tactgactag ccttcaatat
    20461 ttcttgcttc attgatacct tttgctagtg cttccattaa ggatagttct ttgtctgtaa
    20521 agctatccat gtatttttct acctgtaacc ttcgagtgct tttaatcaaa ttatctgtag
    20581 gtaagaaaaa ttcatcaata gatacatgga gtaatgatac aagatcatac agcacttgtg
    20641 tacttgggtg ttgtcctttg ttttctatat ttgttaaata acgtgggtca atttcaatca
    20701 ttgttcccac ttgttcacga gtgagacccc gtttcattcg agcctcttta atcgctaaac
    20761 caacggctct aaaatcgtat ttatcttctt ttttacgcat actaaatcac ctctatacat
    20821 tttactgttc ctatataacc ataaacaggt atagaaaaac atgttatata gtttatgagt
    20881 tcctatagaa cactgtggaa tttattaatg cgatgaatcc aatttcaaat agaaaggtcg
    20941 ctgattttat ttacctatac aactttataa aatttagtca tactcctaga aagtagataa
    21001 aattggcgac ttaatggagg gaaaagtcgc catgagaaag ataatctgtc atctagttct
    21061 cttaattaag ttcttatcaa aaaaacttat ccacctgtac tggatagata tgaccatcaa
    21121 tgtggttttg cagattacat agatagaaaa atcaaagcat atgaacagag aaaacaatct
    21181 gtttatgtgc ttttttggtc acttaataaa tatcttttag gatttctctt catttatgca
    21241 acaaatcccc cgttcgtgtc ggggtttagt atgaagggag ataaagaagc acatgccact
    21301 ccctttcacg acagaaaggg ggtgaaaagc atgaaaccat cttcttttca agaaaccata
    21361 gaaaatcagt ttgactatat ctgcaaacaa gttattgaag atgaacgaaa agattacttc
    21421 aaacatctag acagactatc agcaaaagaa gtctcgtttt ctgatataga taactacctt
    21481 gtcaactctt tttctactgt tgatacgtac gtcactgatt tccaactatt taaattattt
    21541 ggattaagaa tcaatgttga aagtgattta ttaagtgaag ccctgcgtca tctttcagag
    21601 aaaaaacgga acattatttt acttcattac tttatggata tgagtgatgt ggagattgcg
    21661 gagttactaa atttgaatcg ttcgactgtt tatcgtcatc gaataagtgg actagccatg
    21721 attaaagaat tcatgaagga gtgtaaagaa tgaaaacagc ttatccaagg gttcccttcc
    21781 ccctcattgt aaaggcgaca gatggcgacg tggaagcaat taatcagatt gttaaacatt
    21841 atcgagggta tacctcaaaa cgttcattac gtcgcatgac agacgaatat ggaaattctc
    21901 atatggttat tgatgaaacc ctacgtggca gaatggaaac gaaactcatc tctaagattt
    21961 tatcgttcga aattaaatag tcccattttt tcgtggaagc gtgtcgaatc gtatccacgc
    22021 ttcccgattt gggaggtttg ttattccatc aaagcatatt gtgtgttcag taggttttga
    22081 taggctaaca agccaaacgt tctttgacaa ccaaataaag gcaatcgaat acgtttcggt
    22141 aagaaaagag ctgatgaact aacacgccat gacctacaaa attagtgagc gattcatgtt
    22201 agtaattcga aagtaatctt tagcaggatt gctgccatga ctttcttatc gtgataatga
    22261 tactcccgta tagccatagt ccgagcgtta gaagcgtcac aggcaatggg tacggctaca
    22321 tgagaaccat gcagaagtga aattcctgtg agctttgcta gagctgttcg attgctaaga
    22381 tattcttctc ttgtcagtgt attattataa aactatttga tataattata ggtgaatatt
    22441 ggagttttcc tagcctataa ttgggaaagg agtaataatg ataacggtca caaaaaagga
    22501 tttagttgag ctaggatatg gaacttcttt ttcagcagac atcataaaaa aagcaaaaga
    22561 attgatgatt acaaaagggc atatctacta tcaatctcga aagctagata gagtccctcg
    22621 tgaagctgtt gaagaaattc taggaattcg gttttcaaat gaaaacaggt ctgagtagtt
    22681 tttgcacagt aaggagtaaa aactatggtt aaagacccaa taaaaaaagc aaagaatgga
    22741 acctattatt ttcgtgcaaa tctaggttat gattctaaag ggaaaaaaat tcaaaaatat
    22801 cgtagtggat tcactactaa aaaagaagca agagaagaat attcgaaact gctattgatg
    22861 aaaccagaag aattaagtga gaataaggat aaaatgacgt ttaaacatta tatctttgaa
    22921 attttccttc catggtataa gacacaggta aagctcagaa catatgaaaa tcgcttacca
    22981 actattaaga agcatttctc ttattttgat aaaatggctg tttccgatat tgagcctatt
    23041 gatgtgcaaa attggcaact aaaattatca aagaagtgta agtcttctta tgtaagagct
    23101 gttcaaggac tattttcagt tgctatggat cgagcaattg tccttgggat aaccaccaca
    23161 aatccatcta agattattgg caatgtgaaa aagcaaaagt ctaaaattga attttggaca
    23221 aaagaagaat ttgaaaaagt aatctctcta atttacaaag aagattacta tcaacacttt
    23281 ttatttatct ctctgtggtt tttatttatg actggaatgc gaattggtga agctactgcc
    23341 attcaatggg aagatattga ttttgactca ggagtactaa ccatcaacaa aacactctat
    23401 tacaagaatc aatcgaatta tagatttgtt gaaccaaaaa caaaggcgag cgttcgtcat
    23461 attgtattag ataaatgcac attaacttat ttatccgaat ggaaggaagt acaacaaaat
    23521 ctaattcaga cagactttgt aatgagctat aacggtattc ctacccaaaa acatacacta
    23581 tcatatgcta ttgaacgcta tgctaagctg gctggtatcc atcgaatcag aatccatggg
    23641 ttacggcatt ctcatgcttc cttgttaatc agtatgggag aaaatccact tatcattaaa
    23701 gatcgtctag gacacgaaga tattgagacg actttaggaa cgtatggaca tctttatcct
    23761 aacagtaatt tcgaggtagc taacaaatta aatggagcca tttcctttaa agaagcaaaa
    23821 caaaattatg atacttcacc aaagaatcaa tttactgtag actacttgcg aaacaaagaa
    23881 atgaaaaaag tgcaataaaa gtgcagtaaa aagataagaa gagccagaaa cccttggtat
    23941 aagaggattt ctagctctcc tacaactatt cccactccac tgttgctggt ggtttagaag
    24001 tgatgtcata cacgatacgg ttgacgtggg ctacttcgtt tacgatacgt acagagattt
    24061 tttgcaagac ttcccatggg atacgggcaa agtcagcggt cattccgtcg attgaagtta
    24121 cagcacggat accgactgtg tagtcgtaag tacgtccgtc acccataaca ccgacagagc
    24181 ggatccctgg aagaacagtg aaatattgcc agatatcacg gtcaagtcca gcagctgcga
    24241 tttcttcacg caagatagca tcagattcgc gtacgatttc tagcttgtct tcagtgattt
    24301 cgccaagaac acgaatacca agaccaggac cagggaatgg ctgacgccaa acgattgaat
    24361 caggcatgcc taactgagtc cctaattcac ggacttcgtc tttgaataat gtgtttaatg
    24421 gttcgatcaa ttcaaattgc atatcttctg gcagtccacc tacattatgg tgagatttga
    24481 tcgtctgtgc tgtttctgta ccagattcga taacgtcagt atacaacgtt ccttgtgcta
    24541 ggaaagagac gccttcttca ccagcaagtt ttgttgcttc gtcatcaaaa acatatacga
    24601 attcattccc aatgatttta cgtttttgtt ctgggtcaga tacacctttc aacttgtcta
    24661 agaagcgatc tcttgcatcg actttgatga tgttcaagcc gaattttccg cctagacttt
    24721 ccattacttg ttccgcttcg cctttgcgta gcagaccgtg gtcaacaaag atagatgtca
    24781 attggtcacc gatcgctttt tgtaaaagaa ccccaacgac actagaatca acgccacctg
    24841 aaagaccaag caagactttt ttgtcaccaa cttgttcacg gattttttct acttgcatat
    24901 cgataaagtt atccattgtc cagttacctt cacagtgaca aacatcaaag gcaaagtggc
    24961 gcagcaattc gattccgtat tcggaatgac gtacttctgc atggaattgg ataccataaa
    25021 aattacgtgt tgtgtcttgg atagaagcaa tcggacaatc tttgcttgtt ccgactgttt
    25081 caaagccttc aggaacttta gttaccaagt caccatggct catccatact gtttgttgtt
    25141 ttggtgtttc tttaaacaag acagcttgat cgcttgttac ttctaacaag gctttcccat
    25201 attcgcggtt tttagcaggc tctacggaac cgccaagttt gtacgtgatc aattgcatac
    25261 catagcaaat ccctaataca ggaataccta aattgaagat ctcaggatcg atcccaaatg
    25321 cattctcatc atatacactg tttggtccgc cagaaagaat gattcccttt ggattcatcg
    25381 cttttacttc ttctgcagta atgcggtggc tcaataattc ggaaaagact ccaagatcac
    25441 gaatacgtct tgtgatcaat tggttatact gactaccgta gtctaatacg ataatctttt
    25501 caacgttcgt catatcggca acgtttgtca aatttattac ccctatccct ttaaatttta
    25561 tctcaaaata gtcaaactat tttaaaatgc taatattttc gcatgaaaat cgaatcgatg
    25621 atatgatttt cagttttatg gagaattata tcggctcttc ctcgtgtagg caagatatat
    25681 tcctgtaaat ttttcaaatt cacatttttc catacatctt ttgccatttt aaaggcttct
    25741 tcacgatctc cgatagcaaa acgataataa taattctctg gattttggaa agccgtatcc
    25801 agtaacgcac caaaacgatc caaataccat ttttcgatca gcacgggatc ggcatcgaca
    25861 tagacagaaa aatcaaaaaa gtcgctgacg tagatttgct gattcgcagg taattgaagc
    25921 gtattgatcc cctcaacgat caagatatca ggctgctgga tcaattcgta cttgtctgga
    25981 atgatatcat acacatcatg tgagtaaaca ggtactttta tctcttcctg accagatttt
    26041 acacggttca agaaatcgat cagcatctcc atatcataac tttcaggaaa tcctttacgg
    26101 tccatgattc cacgctcttt taatatacga tttggataaa gaaatccgtc agtcgtgatc
    26161 agctgaacgt ttcttcgttt gaaggtacga gacaaaatcg tctgcagcag tctggctgtt
    26221 gtactttttc caacggctac acttccggcg atcccgataa taaaaggcgg tacttctacg
    26281 tatttatgaa gaaataaacc tttactcaag aacaaagatt caaattcttt catataaaga
    26341 taaatcagat gagcaagagg tacatagata tctcgaacat cctgcatgga aatgcggtca
    26401 ttcaaacttt tgatattatc tagttcatct tgtgtcaaag gtgcttgccc attacgataa
    26461 aattctcgcc attcttctct tgccacacgg tagtagttca ttggatcatt catattagcc
    26521 tcctgcgttc tcccatcaat ggaactattt taacacaaaa taaacgcaaa ccccaccact
    26581 taagagagat tgccgatctt tttgtataat ctttataata gaaagaaacg gagtcttatt
    26641 cttgacttgc gcacggatac ataaaaggat agactgttgt catgaaagaa atgagaagga
    26701 tggaagacat gacaaatcat tactattctg aacgcccaga tacggcgcac gattttgaac
    26761 aatgggcttt tgagttaaaa ggcagaacct ttcaatttgt aacagattct ggtgtatttt
    26821 cacgtgaaac agtggattat ggttcacgtg tcctgattga tgctttcgat tgggaagagt
    26881 tacctgatga aggaaagatc ttagatgtgg gatgcgggta tggaccgatt gggttagctt
    26941 tggcatttgc gacacagcgg tttgttgaaa tggtcgacat taatgaacga gcggtaagtc
    27001 tagcgcaagg aaacgcaaag cgtaaccaaa tcgaacatgt tgatatccat caatcgaata
    27061 tctatgaagc agttcatgaa gaaacctatg cagctatcgt aagcaatccg ccgatccgtg
    27121 ctggaaaaaa agttgtacat gaaattttga caggcgcata tcctcggttg aagaagggcg
    27181 gtacactgac aattgtgatc cagaagaaac aaggagcgcc gagtgcgcaa aagaaaatgg
    27241 aagaaacatt tggtaatgca gaaatcgtca caaaagataa aggatattat atcatcaaaa
    27301 gtgtgaaaga ataactagct gaaaaatagt ggagcgacga aagagtggaa ctgggcgtgc
    27361 atgtacgggt acgtatcagt tctttttttg ttgagaagtt tttaaaaaca aagaactatt
    27421 ttttcttcaa aaagaaatag taggaacaga gtcgttgttt tttacgataa aataaataag
    27481 atacagaact tgtatagaag gctgccaagt cggtagataa aggaggtttt tatcaaaaaa
    27541 catactttcc ttttttctct attcttttct tgggggtttt ggtacactga tagggaattt
    27601 agatagaagg gagccaatag gatgaagctg accatcagag atatcgcaga aatggcaggc
    27661 gtatcagtaa caactgtctc acagattttg aataacaaag gaagcagatt tagtgataag
    27721 acaagaaaaa aagtgttgtc gatcgtgaat gaacatcatt ataagcccga ttatttcgct
    27781 tcaaatgtga tcaatcgaca ttcaaagacc atcggaatga tcgtccctga tgtcactgat
    27841 ttcttcttct ccaaactgat cgaaggtgta gagagctatt tgaatccttt gggctatgtg
    27901 atcatgttat gcaattctcg tcatagccaa gaaaatgaga tcaagtattt acaagagctt
    27961 tcccaccgct cagtggacgg aatcttgctg gccacaccaa atattttacc tgagaaatat
    28021 gcattagaca gcggttttta tcaaaaaatg ccaatcattt taatcgatcg cggattgaat
    28081 cgtcgggata acggacggct tatcgtaaag gaatatgagg gcgcctatca agctgtttcg
    28141 ctgtttattg aaaatggcca tacgaagatc ggcatgctga aggaaaccac tggatactat
    28201 caattagaag aacgtttcaa cggatatcgt catgcgctga aagatcatgg cctgcctttt
    28261 aatgggaaat ttgttgaaca tggagagttg actgtccaag gtggatatac tgcatcaaaa
    28321 aaattactca aacataaaga tatcacggct attttttgcg ggaatgatgc catggcgatc
    28381 ggatgctatc aggcgatcga tgaattgggc aaaaaaatcc cagaagatat ctcagtcata
    28441 ggattcgatg gattaaagct gtcagaatac atgattccgc aattgacgac agtgcagcag
    28501 ccaagttttg atattggatt ttatgctgca cgtttcctga tcgatacgat cgaatttcct
    28561 caaagaaaag ttccgaacaa agtgttcgaa acaaaattaa ttattcgtga gagcgtaaaa
    28621 gtattgacaa agggctaact cggcggtata ttgcttatgt aagcatttac aaaggctagt
    28681 tagtgctttt tttattttta gtaaaccagt ttactaaagt ggtttacctt tcctaaaaat
    28741 aatgtataat accgaatgaa gacaaggagg atcaggcaat gcgaatggtg gatctgattg
    28801 aaaaaaagag ggatggaaac gagctgtcga aagaggaaat cgagtacatc gtcacgaact
    28861 atacgaatgg aaagattcca gattatcaag tcagtgcatt attgatggca attttttatc
    28921 aagacatgac aaatgaagaa atcacgaact taacattggc gattgctaat tctggtgatg
    28981 tcatcgattt gagttctctt gaaggaataa aggtagataa gcattcgaca ggtggcgttg
    29041 gtgacacgac cacattgatt ttagcccccc tagtagctag tgtcggagtg acagtggcta
    29101 aaatgtctgg acgcggtcta ggttacacag gtggtacact agacaagcta gaagcaattc
    29161 ccggatttca gatcgagctt tcggatgaag aatttgtacg cattgttaat gaaagtaaag
    29221 tagcggtaat tggtcaatca ggaaacttgg caccggcgga caaaaaattg tatgccttgc
    29281 gtgacgtcac agcgaccgtt gattctcttc cgctgattgc cagttcgatt atgagtaaaa
    29341 aaatcgcggc aggagcagat gcaatcgttt tggatgtgac cactggagac ggtgctttca
    29401 tgaagaacat cgaagatgca agacgtctgg caaagaccat gacgagcatt gggaaactgg
    29461 ctaaccgaga aacagtggcg gttatctcag atatgtcaga acctcttggc gaagctatcg
    29521 gaaatagtct agaagttgtc gaggcaatcg aaacattaca aggaaatggt ccagaagatc
    29581 tagtagaaat gtgctacgcc ttaggaagcc aaatggttgt tcttgctggc aaagctaaga
    29641 caaccgatga agcaagaacg ttgcttcaag aagctctgga atcaggtaaa gcgttagcaa
    29701 aattcaaaga aatgatccaa aatcaagggg gagacccagc gatcgtggaa catccagaac
    29761 gaattttgac tgcacgctac acaatggagc ttcctgctaa acaaagcgga gttgtgtcaa
    29821 aaatcgtggc aaatgaatta ggaattgctg caatgatgct aggtgctggg cgtaaaacca
    29881 aagaagaaga tattgatcac gctgttggat tgaaactgca taaaaaaatt ggtgacactg
    29941 tgacaaaagg tgagtcacta ttgacgatct atagtaacga cgaagaaatc acttctgtca
    30001 ttgaattatt atataaaaat attgaaattg gtgaatcggc aatgaagcca acgttgattc
    30061 atgacatcat cacagaataa aggaaaaaag cggactttac cgttttaaag aaaaggggaa
    30121 aagaaatgga actaaaccga atgatcgacc atactatttt gaaagcagac gcatctaaag
    30181 aagctgtgat gcaaattatc gaagaagcaa aaaaatacca tttttattct gtctgcatca
    30241 atccagcatg ggttgcgcta gcgaaagaac aattacaagg tgagccagta gccgtatgta
    30301 cagtaatcgg gtttcctcta ggagcaaaca cacccgagac aaaagcattt gaaacaacag
    30361 atgccatcaa taacggtgca gatgaagtcg atatggtcat caatatcggt gctttgaaat
    30421 caaaggatga tcaacaagtc caaaaagata tcgaagcagt tgttgaagca gcaaaagatc
    30481 gtgcgcttgt caaagttatt attgaaacat cgttattaga tagagaagaa atcatccgag
    30541 cttgcgaaat agcaaaagct gcaggcgctg attttgtcaa aacatcaact ggtttctcaa
    30601 caggcggagc aaaggtagag gatgtccgtt tgatgcgtga aactgtagga ccagaaatgg
    30661 gtgtcaaagc ttccggcggt atccataacg aagaagaagc aatggcaatg atcgaagctg
    30721 gtgctacaag aatcggaaca agcgcaggag tagcaatcgt atctggttca actggtgaag
    30781 gctattaatt ttttcaaagg agaagctcat ggcagaactc aaacaagaat ggatcaacac
    30841 agctattgaa gcgttagata aagcctatgt tccttattcc cattttccgg taggcgcttg
    30901 tctcgtaacg gaatcaggca agatttatca aggcatcaac attgagaatg catcatttgg
    30961 attaacgaat tgtgccgagc gcacagcttt tttcaaagca gtatctgaag gagaaaaaac
    31021 atttacgcat ctagtcgtag cggggcacac ccccgatcct atctctcctt gtggtgcatg
    31081 tcgacaagta atggcagaat tctgttcccc tgatatgccg gttacgctag taggagacaa
    31141 tggtgtgacg aaggcaacga ccgttcgtgc actgctgcct tatgcattta ctgaaaaaga
    31201 tctataactc atgttcattc agtcagctga ctgttgaaaa ataaaacaat tattttagga
    31261 ggctttacag aaatgaaaaa agcaaaatta tttggtttag gtgctgtcgc actagcagca
    31321 gggttatttt taggtgcatg cggcaacaac ggctctactg attcatccgg tggcaaaagc
    31381 tcatcagaca caacaactgc agcattgatc acagacacag gtggtgtaga cgaccgttca
    31441 ttcaaccagt cagcttggga aggactagaa aaatggggga aagatcaagg actttcacga
    31501 ggaaatgatg gcttccaata tttccaatct tcaaatgaat cagattacat cccaaatatt
    31561 gaccaagcat tgaatgctgg attcaaaacg atttttggta ttggttataa actaaaacca
    31621 gcgatccaag aacaagcaac aaacaataca ggtacgaact ttgttattat cgatgatgtc
    31681 atcgatggat tagataacgt tgtatctgca actttcaaag acaacgaagc ttcttatctt
    31741 gcaggtgttg cagcagctta tacgactgaa acaaacgtag tcggctttat cggcggtgta
    31801 aaaggtgaag taatcgatcg ttttgatgct ggattcaaag ccggtgttga tgcaggtgcg
    31861 aaagaattag gaaaagaaat caaagtattg aatcaatatg ctggtgactt ctctgctcca
    31921 gataaaggac gttcaattgc tcaaggtatg tatgcacaaa acgcagatat catcttccat
    31981 gcttcaggcg gtacaggtaa cggtgtattc caagaagcaa aatcattgaa cgaatcaggc
    32041 gacaaaaaag tttgggttat cggtgttgac cgcgaccaat cagacgaagg cgaatacaca
    32101 ttaaacggcg aaaagaaaaa cttcacattg acatctacac tgaaagcagt tggaacagta
    32161 gtagaagatt tggcacaaaa atcagcggac ggtaaattcc ctggcggcga acacactgtt
    32221 tatggattga aagaagacgg tgttggtcta acagaaggac aactttctga cgaagcgaaa
    32281 aaagcagttg atgaagcaaa agaaaaaatc atttcaggtg acgtaaaagt tccagaaact
    32341 cctgaagaaa actaaaacca tttctatagc cttgttagac acatgtttga caaggctatg
    32401 gtttgattag aacaagaatt gttcatttat ccaagctgcc ttgtgacagt ttggataagg
    32461 gaacaactcc ctgaagtaat gtttgtttca aaagaagaac gctcactaag cgtgaatctt
    32521 agtcaatcgt tgccctagat agaaatcgtt aggatgtgaa aaaaaggtgg ttcaagaaaa
    32581 tattgtcata gaaatgcaga atattaccaa agaatttgga acattcaagg caaatgacaa
    32641 catcaattta caagtaaagg caggagaaat tcatgcgttg cttggagaaa acggtgctgg
    32701 caaatctaca ttgatgaacg tgctttccgg attattagag ccgacatcag ggaaaatttt
    32761 gatgcgtggg aaagaagtaa agatcacaag tccgaccaaa gccaaccaat tagggattgg
    32821 gatggtccat cagcacttta tgcttgttga tgcctttact gtaacagaaa acatcgtgtt
    32881 gggaagcgag cctagtcgcg cagggatgct tgaccataaa aaagcacgaa aagagatcca
    32941 aaaagtttct gaacagtatg gattgtcagt caacccagat gcttacgttc gtgatatttc
    33001 agttggaatg gaacaacggg tagaaatttt aaaaacactt taccgaggag cagatgtact
    33061 gatttttgat gagccgacag ctgtattgac ccctcaagaa attgatgaat taatcgtgat
    33121 catgaaggaa ttagtcaaag aaggcaagtc gatcattttg attacgcata agttagatga
    33181 aatcaaagca gtagctgacc gctgtacagt tatccgccgt ggaaaaggaa tcggtacagt
    33241 caacgttaaa gacgtaacct cacaacaatt agccgatatg atggttggaa gaacggtttc
    33301 attcaaaacg atgaaaaaag aagcgaagcc tcaagaagtc gttttgtcta ttgaaaatct
    33361 agtggtgaaa gaaaatcgtg gattagaagc cgtgaaaaac ttgaacttag aggttcgtgc
    33421 tggcgaagta cttggtatcg ctggaatcga tggaaacggg cagtcggagt tgatccaagc
    33481 tttgactggt ttgcgaaagg cagaaagcgg acatatcaag ctaaaagggg aagacatcac
    33541 taataaaaaa ccccgaaaaa tcactgaaca tggtgtagga cacgtgccag aagaccgtca
    33601 taaatacggg ttagtcctag atatgacatt atctgaaaat atcgcactac aaacctatca
    33661 tcaaaaacct tacagtaaaa acggcatgct gaattattca gtaatgaatg aacatgccag
    33721 agaattgatc gaagaatatg atgttcgaac aacgaatgaa cttgttcccg caaaagcttt
    33781 atcaggcgga aatcagcaaa aagcaattat cgctcgtgaa gtcgaccgag atcctgatct
    33841 gttgattgtt gcaaatccaa ctcgtggact ggatgtaggt gcgatcgaat ttattcataa
    33901 acgtctgatc gaacaaaggg acaaatacaa agcagtgtta ctgatcagtt tcgaattaga
    33961 agaaatttta aatgtttcgg atcgtattgc tgttatccat gaaggagaaa ttgtcggtat
    34021 cgttgatccg aaagaaacat ctgaaaatga attaggtcta ttgatggcgg gctatacctt
    34081 agaagaagca cgaaaagagt tagaaaaggg ggctggcgca catgaatgat cgcagcgaaa
    34141 aattccgtaa tttattagtg cctgtacttt cagttgtttt aggattgatc ttaggcgcga
    34201 tattgatggc tgcttttggt tttcagccca tccaaggtta ttcttctatg ctagatgcag
    34261 cccttggttc gcaaagaggg ataggtgaaa cgttaaggca agcaacgccg ttgattttta
    34321 cagcattagg tttttctgta gctaactcag ctgggttttt caatattggg ctttctggac
    34381 aagcactttg cgggtggatc gtcagtgtct gggtggcact tgcgtttcca gatctgccaa
    34441 aagttgtttt gcttccgtta tgcgtgatct taggtgcctt agctggtgcg atggcagcgg
    34501 ctgttccagg tttacttcga gcattttttg gaacaagtga agtgatcgta acaatcatga
    34561 tgaactatat cttattatac gtaagcacat ttgctttaca tgaagtgatg cctgaaagct
    34621 atcgctcaag tttggactct tccaatatga tcagtcaaaa tgcctcactg cggttaccat
    34681 ggttaacaca agcttttggc ggctctcgcg taaactcagg attattttta gcaatcatcg
    34741 cgcttatttt cgtttggctg ctgatgaaga aaacgacttt aggatttgaa atccgttctg
    34801 ttggattgaa tccttttgct tctgagtatg caggaatgag tagtaaacgt acgatcgttt
    34861 tgtcgatggt catttctggc gctttggcag ggcttggcgg tgtggttgaa ggattaggaa
    34921 cgtatcaaaa cttctttgtt caaacgactt cgctatctat tggatttgac ggaatggctg
    34981 tttccttgct tggttctgga tcagcaatcg gtattctttt atcagcgatc ttgttcagta
    35041 ttttgaagat cggtggactt ggcatgcaga caggagcagg tgttccgttt gagatcgtca
    35101 atgtatcgat tgctttgatt atcttctttg tcgggatcaa cttcctgatt cgtttcgtca
    35161 tggctaaagt cttccaaggg aaaaaacaag aagagatcgt tgcgacaata gaatcgaaac
    35221 caacgaatca aaaccatgaa gggggcgaac tataatgtcg actatcgaat taattgcctt
    35281 gatccttact tctacactgg tttattcaac accgctaatt ctaacgtcac taggcggaac
    35341 tttttctgaa cgaagcggga tcgtaaacgt agggctagag ggaatcatgg tcatgggtgc
    35401 ttttagttcc atcgttttca acttgacgat gagtccatca cttggttcta tgacaccatg
    35461 gatcgctatt ttagtgggtg gcgtcgtagg tatcctattt tctctgattc acgcagtagc
    35521 aacagtcagt ttgcgtgctg accatattat cagcgggaca gtaattaatc tgatggcacc
    35581 tgctttaggt gttttcttga tcaaagcatg gtatggtaaa ggtcaaactg ataacatcac
    35641 gcagaatctg gggtattttt ctttcccagg attagcagat attccggtca ttggaccaat
    35701 tttctttaaa aatacattct tacctgctta ttgtgctatt ttgattgctg tcctagcttg
    35761 ggtgatcatc ttcaagacga aattcggctt gcgcctccgt tcagttggtg aaaatcctca
    35821 agctgccgac acattaggta tcaatgtata tgctatgcgt tattcaggcg tactgatttc
    35881 tggcttgtta ggcggtatgg gcggtgctgt ttttgctcag tcgatttccg gtaacttttc
    35941 agctggtacg atcgttggac aagggttcat ctccatggct gctatgatct ttggtaagtg
    36001 gaatccttta ggggcaatgg gtgcctcatt gttctttggt tttgctcaaa gcttgagtat
    36061 cattggcgca caattaccag ttatttcttc gattccgcca gtattgctgc aaattgctcc
    36121 atatttgctg acaatcattg tgctggttgt tttcttaggg aaagcttctg gtccaaaagc
    36181 aaacgggaag aactatatca aatcaaaata atcttttagc gaataaaaag ggtgggttac
    36241 gggaagtgtt tttcgtctcg ccctttttgt tttaaaggca ataaaaataa aaagaaagaa
    36301 ggaataatca tgtttaaacg cgtacatctg atcgtcatgg attcagtagg aattggagaa
    36361 gcaccagatg ccgataagtt cggtgatgtt ggttctgata cattaggaca cattgcgaaa
    36421 gaagcaggat tgaacgttcc acatttagag cagcttggtt taggaacgat ccgtcctctt
    36481 gaaggtgtgg aatcagtgaa agatcatcaa ggctatgcga cgaaattaga agaagtttct
    36541 gtagggaaag atacgatgac aggccactgg gaaatcatgg gattgaatat caaaactcca
    36601 tttcgtgtgt ttcctaatgg attccctgat gatctgatca aacaaatcga agaattttct
    36661 ggccgtaaag tcgtatgtaa caaaccttat agcggaacag ctgttattga cgattacggt
    36721 gaacaccaaa tgaaaacagg cgatttaatt gtatatactt ctgccgatcc tgtccttcag
    36781 atcgcggcac atgaagaaat cattccttta gaagaattat atcggatttg ccaattcgtt
    36841 cgtgatataa caaaagatga tccgtatatg attggccgaa tcattgcacg tccgtacgtg
    36901 ggtgaaccag gagacttcac acgaacaagc aatcgccatg attatgcgtt gaatccattc
    36961 ggacacacag tccttgattc tttgaaagaa gcaggaaagg acgtgattgc tgttggaaag
    37021 atcaacgata ttttcaacgg acaaggaatc actgaatctg tccgcacgaa aagcaatatg
    37081 gatggcgttg accagctgct gaaagttatg aaaaacgatt tttcaggaat cagctttacc
    37141 aatttagtag attttgatgc attatacggt catcgtcggg atgtagtcgg ctatgcgcat
    37201 gcgattgaag aatttgactt acgaattcca gaattgcttg aagcgatgga agaagacgac
    37261 ttgctaatga ttacagccga tcacggaaac gatccgacgt ttactggtac cgatcatacg
    37321 cgagaatatg taccgttatt ggtttacagt aaaaaaatga aagaacaagg ggcattgcct
    37381 caaggatatt attcagatat ttcagcaacg attgctgaga actttagtgt ttcttcgact
    37441 gaaaatggcg aaagcttctt gaaattatta aaataatctt tttaaatagg agaaagcaaa
    37501 tgaccaagtt aagcgagatg ttacaagaaa cgacccagtt tatccttgat aaaggagtaa
    37561 aagaaattga ttttggtttg attctaggtt caggattagg tgaattagca gatgaaatcg
    37621 aagatgctgt aatgatttct tatgatgaga tcccgttctt tcctgtttcg acagtagttg
    37681 ggcatgcagg aaagctagtt tatgggtctc tggcaggcaa gaaagtgtta gccatgcaag
    37741 ggagattcca ctactatgaa ggtcattcca tgcaaacagt gacctatcca gtacgtgtga
    37801 tggccggact aggggcccat tcagtcattg tgactaatgc atgtggtggt gtcaacgaat
    37861 catttgtacc aggagatttg atgctgatta ccgatcatat caattttact ggtgaaaatc
    37921 ctttgatcgg tccgaatgaa gatgcaatgg gccctagatt tccagatatg agtcaagctt
    37981 atacgcctga ctatcaagca acagcaaaag cagcagcaga aaaactaggt ttggatctta
    38041 aagaaggcgt atatatgggc tattctggac caacttatga aacgcctgcg gagatccgaa
    38101 tgtctcggac aataggagcc gatgcagtag ggatgtcgac tgttccagaa gtcatcgtag
    38161 cagctcacag cggattaaaa gtgttaggaa tctcttgtat taccaactta gcagcaggta
    38221 tgcaagcgaa tctgagccat gaagaagttg tagaaacaac tcagagagtc aaacaatctt
    38281 ttaaagcatt ggttaaagaa acattgacat tattgtaata ggaggaacaa taaaatgagt
    38341 gtgcatattg aagcgaaaga aggcgagatc gccgataaga ttttacttcc gggagatcct
    38401 ttgcgagcta aatatattgc ggagactttt ttggaagatc cagtttgtta caatcaggta
    38461 cgcggtatgt taggctttac cggcaaatat aaaggagaac gtatttctgt tcaaggaaca
    38521 ggaatgggaa tgccatcagc caccatctat gcccatgaat tgatccagtc gtatggtgta
    38581 aagaaattga ttcgagtagg tacatgcggt gcattgtcaa aagacgtcca tgttcgtgat
    38641 ttagtactag cacaaggtgc agcgaccagt tcttcgatga tcgaaaaaaa tttccaagcc
    38701 tttcattttc ctccaatcag tgatttcaat ctattactga aagcatatga gatagctaaa
    38761 gaaaagggct atacggttca tgtaggaaat gttctttcgg aagattcttt ctacaaagat
    38821 gatttgacag agacgttcca attagctgaa cttggcgttt taggagtgga aatggaagca
    38881 gcggctcttt attacttagg cgcgaaatac catgtgcaga cattaagttt gatgaccgtt
    38941 agcgaccatt tgatcaccgg tgaagaaaca acagctgcag aacgtcaatc tacgttcaat
    39001 gaaatgatcg aagtaggatt ggaaacagcg atagcagaat aagaaggaaa tgaaaaagct
    39061 gccctgcatt aagtaataag aacatccaag caaaaatagc tcctcatgtt tttgacttgg
    39121 ctggacttat tattgcaaat gtaagctttt gaacaccgtt taccaagagg ttgtgaatct
    39181 gccgtttaac tctgagtcac aaggaacaat ttcaaaaaac agcttttcat gtttgatgaa
    39241 tattgtgact tgtgaccgag aagttggcag gagaaaaccg tttatacgaa aaaagggact
    39301 gtgacaaatc gtgtcacagt ccctttttta tagtgttttt tataggtagt attttttagc
    39361 aactgttaag tcatcattta attcataaac aagtggttga cctgttggaa tttcaagatc
    39421 catgatgtct tcgtcagaga taccttcgat atgtttagct agggcgcgta atgagttacc
    39481 atgtgcagct actaaaacag ttttgttgtc tagtaatgct ggagcaattt catcttgcca
    39541 gaatggcaaa gcgcgttcta atgtcacttt caagttttct ccaccaggaa tatcgcgttt
    39601 gtctaacatt gcgtaacgac ggtcatttgc tgcagaacct tcgtcagttt cttccattaa
    39661 tggaggaaga gtatcgtaag aacgacgcca gatgtgcact tgttcgtcgc cgtatttttc
    39721 agctgtttct tttttgttca aaccttgtaa tttaccatag tgacgttcgt ttaagcgcca
    39781 tgatttgatt tgtggtaccc aaagttgatc tgaatattct aagatcaagt tacatgtttt
    39841 gattgcacga gttaaaacag aagtgtaagc aacatcaaat tcgattccag cttctttgat
    39901 tttgcgtccg ccttcttttg cttcttcgat tccttctggt gctagattta catcagccca
    39961 accagtaaat tggttcaatg cattccattc actaagaccg tgacgagaaa aaactaattt
    40021 tggcatgttt agctctcctt ttaagatagg tttatatttc tgaaattttc agttctgttt
    40081 tattgtacaa ttttttggaa gagatttcca tcgaaaaatt agataagatt ctatgagaaa
    40141 acaagtagag aatataaaaa aacgaacagt gcccacgcaa ctgttcgttt ttaggtatta
    40201 gatgcataat tgctcgattt ctttttcagg taaattgata tatttataac atgttccaac
    40261 tgaaacaccg cattttgaag aaataaattg gatcgtttct tttttttcat aatagagacg
    40321 tcggattttt ttgatcgttt ttgcatcgat ttttggtctt ccgccgattt ttcctttttt
    40381 acgagcttca tctaatccaa ctaatgtacg ttctttgatc aatgcgcact ccatgtttgc
    40441 taggttttcc attaattgga aatagatttt tcccatttcg cgcgtatcga ttccttcatc
    40501 aagactaatc aagtgcagcc cgcgatcttt gaataattgc gtgagttctg ttaattgccg
    40561 cgtagatttg cctaatcgat gaagttggca aatgactagt gcatcgcctt gttgcatgtc
    40621 agcgatcact gactccaaat gaacagcagt agaatcattt tgttccactt catatgtctc
    40681 atggaaaatt tcttgacaac caaagtcaga taattgatca acctgagttg ttaaatcaac
    40741 gtcatttatg gttgtgcgtg cataaccaat aattttcatg gtgattcctt tctagcagtg
    40801 attttctaac tggatataac agtaaaaatg tagcataaag tacgtcaaaa agatagtttt
    40861 ttcgcaaatt ttccgctgtc tttttttatt ggatgatcaa gcagtttttc tttatttacg
    40921 acggattgcg tcaagaatct caacgccttt ttctgagccg acaactactt tagaaaccat
    40981 gtttaggaaa agcccatgtt ctactacccc aacaagttca tctaaatagg tagctaagct
    41041 ttctggctgt tcgatgactt ctagatgcag atcaatgatg taatgaccac catctgtggt
    41101 taagacttct ccgttttcgt ctgtacgcaa gacgggttgg aatctttttt cttcaaaaat
    41161 atgaagtagc tgttgggagc cataagggat cacttcaact gggagtggga acttcccaag
    41221 tttatcgact aatttagaag aatcaacgat ccaaatcgtt tcttttgagt acgtagcgac
    41281 tattttctcg aaaagcaaag cagcgccgcc gcctttgatc ccttgaaaat cagcactgat
    41341 ttcatcagca ccgtctattg tcaaatcgac tacggggact tcatcaatac tctttaacgg
    41401 tattcctaat ttttccgctt gttcttttgt cgcatttgaa gtagtaactc ctgtgatacg
    41461 taaaccttct tcacgcatac ggcgaccaat ctcttctacc atataatagg cggttgaacc
    41521 ggtcccgaga cctacgatca tcccgtcctt tacgaatttc gcggcttcaa tgccagctaa
    41581 ttgttttaaa ttcatctttg cgtccacctt tttttagttt gagcatcgtg ctttcctcac
    41641 tattttagcg tgatttacgg taaaaagata ccattctttt ttattccaat aaagcttttt
    41701 caaaaaaaat tgcatctctt gttgacatgt tttttcattc gtgatattct aacaaaggtg
    41761 ctttatctac aggtttctgt gaagaaacgt gctgactgtt tatcaaagca tatttgataa
    41821 ggatgcagac attgcattta ttttttatca caaaaaaaga accacctgga tgtgtggaac
    41881 tagacgcaaa tcgtgggaag gaggaaacaa ggaatgccta caattaacca attagtacgt
    41941 aaacctcgta aatcaaaggt ggaaaaatct aattcacctg ctttgaacaa aggatataac
    42001 agctttaaga aaactcaaac taacgtaaac tcacctcaaa aacgtggggt agcaactcgt
    42061 gtgggtacaa tgacacctaa aaaaccgaac tcagctttac gtaaatatgc acgtgttcgt
    42121 ttgtcaaact taattgaagt tactgcttat atcccaggga ttggtcataa cttacaagaa
    42181 cacagtgttg tattattacg cggtggtcgt gtaaaagact taccaggggt acgttaccat
    42241 atcgttcgcg gtgctttaga tactgctggt gttaacgacc gtaaacaaag ccgttctaaa
    42301 tacggaacta aaaaacctaa agcttaattt taattattaa acaaatgaaa cttgcttcta
    42361 ctatatagaa gtaattacta tgaacaatat ttcggaagga ggagttacgg atgccacgta
    42421 aaggtcctgt tgcaaaacgt gatgttttac cagatcctat ttataactca aaattagtaa
    42481 ctcgcttgat caaccgtgta atggttgacg gaaaacgcgg gattgcagct aacattatct
    42541 ataatgcctt tgacttgatc aaagaatcta caggtaacga tccattagaa gtgtttgaac
    42601 aagcaatgaa aaacgttcaa cctgttcttg aagttaaagc acgtcgtgtt gggggttcaa
    42661 actaccaagt accagttgaa gttcgccctg aacgccgcgt tactctaggt cttcgttggg
    42721 tagtaaacta cgctcgcttg cgcggtgaac acacaatgga agaacgctta gcaaaagaaa
    42781 tcatggatgc tgcaaacaac acaggtgctt ctgttaaaaa acgtgaagac actcataaaa
    42841 tggctgatgc caaccgtgcg tttgctcact atcgctggta agattctctc tgtttggtcg
    42901 gcttttataa gtcgacctca gatagtgtcc ttatttttag caactagata gtaaagagag
    42961 gagaaacaag gagaatgacc agagaatttt ctttacaaaa cactcgtaat atcggtatca
    43021 tggcccacat cgatgctggt aaaacgactg ctactgaacg tatcttgtac tatactggta
    43081 aaatccataa aattggtgaa actcatgaag gagcttcaca aatggactgg atggaacaag
    43141 aacaagaacg tggtattacg atcacttctg ctgctactac agcacaatgg aagggtcacc
    43201 gaatcaacat catcgacaca ccaggacacg tagacttcac agttgaagtt gaacgttcat
    43261 tgcgtgtact tgatggtgct gtaactgttc ttgatgcaca atcaggtgtt gaacctcaaa
    43321 ctgaaacagt ttggcgtcaa gcaacaactt atggtgtacc tcgtatcgta tttgcaaaca
    43381 aaatggacaa gatcggtgct gacttcttat attcagtatc aactttacat gaccgtttgc
    43441 aagcaaacgc tcacccaatc caattaccaa ttggtgcgga agacgacttc acaggaatta
    43501 tcgaccttgt aacaatgaag gctgaaatgt atacaaacga tcttggaaca gagatcgaag
    43561 aaacagaaat tcctgaagaa taccgcgaat tagctgaaga atggcgcgaa aaattggttg
    43621 aagctgtagc tgaaactgat gaagaactta ctttgaaata cttagaaggt gaagaaatca
    43681 ctgaagctga gttaaaagaa ggtatccgcc gcgcaacagt taacgtagaa ttctacccag
    43741 tactatgtgg ttcagcattt aagaacaaag gggttcaatt actattggac gctgttcttg
    43801 attacttgcc atcaccgtta gatatcccag ctatcaaagg gattgaccct aaaactgatg
    43861 aagaagtaga acgtcctgca gacgacagcg ctcctttctc agctttagca tttaaagtta
    43921 tgactgaccc attcgtaggt cgtttaactt tcttccgtgt gtactctggt gttttacaat
    43981 caggttcata cgtacaaaac gcaacaaaag gcaaacgtga acgtgttggt cgtatcctac
    44041 agatgcatgc caactctcgt tcagaaatct ctgaagttta cgctggagat atcgctgctg
    44101 ctgttggttt gaaagacaca actactgggg acacactatg tgatgaaaag agtctagtta
    44161 ttcttgaatc catggaattc ccagaaccag ttatccaagt cgctattgaa cctaaatcaa
    44221 aagctgacca agataaaatg ggtgtggctt tacaaaaact atctgaagaa gatcctactt
    44281 tccgtgctga aacaaacgtg gaaacaggcg aaacaatcat cgctggtatg ggtgaacttc
    44341 acttagatat catcgttgac cgtatgcgtc gtgaattcaa agttgaagct aacgtaggtg
    44401 cgcctcaagt atcttaccgt gaaactttcc gtgctggcac tcaagccgaa ggtaagtttg
    44461 tacgtcagtc tggtggtaaa ggtcaatacg gtcacgtatg gattgaattt acaccaaatg
    44521 aagaaggtgc tggtttcgaa tttgaaaatg cgatcgtcgg tggggttgtt cctcgtgaat
    44581 acattccagc tgttgaaact ggtttgaaag atgctatgga aaatggtgtc ttagctggct
    44641 atccattagt agatatcaaa gctaaacttt acgatggttc ataccatgat gtcgattcaa
    44701 atgaaactgc cttccgtgtt gctgcttcta tggcattacg tgcagcagct aagaaagcta
    44761 atccagtaat ccttgaacca atcatggctg tagaagttgt tatccctgaa gattatttag
    44821 gtgatgttat gggtcacgta actgctcgtc gtggacgtgt tgaaggtatg gaagctcgcg
    44881 ctggcggcca acaagttgtt cgtgcgatgg ttccacttgc tgaaatgttt ggttatgcta
    44941 caactcttcg ttctgctact caaggtcgtg gtacatttac aatgacattt gaccactacg
    45001 aagatgtacc aaaatcagta caagaagaaa tcattaagaa aaacggcgga aaagctgaat
    45061 aatttcttaa aaccggatag tcctgcaaag agattatcca aagagactat atttttaata
    45121 tagtttcgtg taaaataatt atcgatgata tgggcatgtg gtgtaccttg tgtacatcat
    45181 atacctatca gacaaataaa aaatatattg tatttttagg aggacatttt aaaatggcaa
    45241 aagaaaaatt tgaccgttct aaaccacacg ttaacattgg tactatcgga cacgttgacc
    45301 atggtaaaac tacattaaca gctgcaatca caactgtact atctaagaaa aacggcggcc
    45361 aagctatggc ttacgatcaa atcgatggtg ctccagaaga acgcgaacgc ggaatcacaa
    45421 tctcaacagc tcacgttgag tatgaaacag atacacgcca ctacgctcac gttgactgcc
    45481 caggacacgc ggattacgtt aaaaacatga tcactggtgc tgctcaaatg gacggagcta
    45541 tcttggtagt ttctgctgct gacggcccaa tgcctcaaac tcgtgaacac atcctattgt
    45601 ctcgtcaagt tggtgttcct tacatcgttg tattcttgaa caaagtagac atggttgatg
    45661 acgaagaatt actagaatta gttgaaatgg aagttcgtga cctattaaca gaatacgaat
    45721 tccctggtga cgatgttcct gtagttgctg gatcagcttt gaaagctcta gaaggcgacg
    45781 cttcatacga agaaaaaatt cttgaattaa tggctgcagt tgacgaatac atcccaactc
    45841 cagaacgtga caacgacaaa ccattcatga tgccagttga agacgtgttc tcaattactg
    45901 gacgtggtac tgttgctaca ggtcgtgttg aacgtggaca agttcgcgtt ggtgacgaag
    45961 ttgaagttgt tggtattgct gaagaaactt caaaaacaac agttactggt gttgaaatgt
    46021 tccgtaaatt gttagactac gctgaagctg gagacaacat tggtgcttta ctacgtggtg
    46081 ttgcacgtga agacatccaa cgtggacaag ttttagctaa accaggtaca atcacacctc
    46141 atacaaaatt ctctgcagaa gtatacgtgt tgacaaaaga agaaggtgga cgtcatactc
    46201 cattcttcac taactaccgt ccacaattct acttccgtac aactgacgta acaggtgttg
    46261 ttgaattacc agaaggaact gaaatggtta tgcctggcga caacgtaaca atggaagttg
    46321 aattaatcca cccaatcgct atcgaaaacg gtactaaatt ctcaatccgt gaaggcggac
    46381 gtacagttgg tgccggtgtc gttacagaaa tcaaagctta attcacttaa gttttaaagt
    46441 aaagcaacat ttattcggac gtaatcgctc ggaacgaagg tcttaggatc tttgttccga
    46501 gcttttttat ttattgcagg atcaggaaaa aagacaggag tattataaat agaagaaaca
    46561 cgaaataaaa gagtcagtat tttctaaatg aaactttatg aaaacaaaat gttttttaca
    46621 agaaaaagcc ttgctttgtg agtttatata tattataatg acaaaggtgc gtttacttta
    46681 gtgaattgca cagacgtaat cagaatgatt tcgtcggcta agaaacgaga ggttgcgaca
    46741 cgcccggacg ctttgccacg aacgagcgtg acggaaattt tcgtggagct atgtctactt
    46801 ttcaaaatag gcgaaggagg gaacaagatg gcaaaacaaa aaattcgtat ccgtttaaaa
    46861 gcgtatgaac accgtatttt agatcaatca gcggataaaa tcgtggaaac tgcaaaaaga
    46921 actggagcta gcgtgtctgg tccaattcca ttaccaactg aacgcagtct ttacacaatc
    46981 atccgtgcga ctcataaata caaagattca cgcgaacaat tcgaaatgcg tacacacaaa
    47041 cgtcttatcg acattgtgaa cccaacacca aaaactgttg atgctttgat gaagctagac
    47101 ttaccatctg gtgtaaacat cgaaatcaaa ttataaaact aaaacaaaaa taatggaggt
    47161 gtactcatga ccaaaggaat cttagggaaa aaagtgggaa tgacacaaat cttcactgaa
    47221 tctggtgaat taattccagt aactgttgta gaagctactc caaacgtagt actacaagtg
    47281 aaaacaatgg aaacggatgg ctacgaagct attcaagttg gttaccaaga tatgcgtgaa
    47341 gttttatcaa acaaacctgc gaaaggtcat gttgcaaaag caaacacggc tcctaagcgc
    47401 ttcattcgtg aattcaaaaa tgttgagcta ggagaatatg aagtaggaaa agaaattaca
    47461 gtagatgttt tccaagcagg agacattatt gatgtaacag gtactacgaa aggtaaagga
    47521 ttccaagggg ttatcaaacg ccacggacaa agccgcggac caatggctca cggttctcgt
    47581 taccatcgtc gtcctgggtc aatgggtcca gttgcaccta accgtgtatt taaaaacaaa
    47641 cgtcttgctg gccgtatggg tggcaaccgc gtaactatcc aaaacttgga agttgtacgt
    47701 gtcgatgcag aaagaaatgt tatcttgatc aaaggtaatg ttcctggagc taaaaaatct
    47761 ttaattacta tcaaatcagc tgtgaaagct aaataattgt aggaagaaag gaggaactaa
    47821 ggaatgccga atgtagcatt attcaaacaa gatggaagcc aaaacggtga aatcacactt
    47881 aacgaagaaa tcttcggaat tgaacctaat gaaagtgttg tttacgatgc aatcgtaatg
    47941 caacgtgcgt cattaagaca aggaacacac gcggttaaaa accgtagcgc tgttcgcggc
    48001 ggcggacgta aaccatggcg tcaaaaagga acaggtcgtg cacgtcaagg atctatccgc
    48061 tcaccacaat ggcgtggagg tggcgtagtc tttggaccaa caccacgttc ttacagctac
    48121 aaacttccta aaaaagttcg tcgtttagca atgaaatctg tattgtcaga aaaagttgct
    48181 gaaaacaact tggtagcaat cgaaggcttg aacttcgacg caccaaaaac aaaagaattc
    48241 aaacaagttc ttgctaactt atctatcgac tctaaagtat tggttgttct agaaaaaggt
    48301 aacgactttg ctgctttatc agcacgtaac ttaccaaacg tttctgtagt aacttctgat
    48361 aatgtgagtg tgctagacgt tgtatcaaat acaaaagtgt tagcaacaca aactgctctt
    48421 actcaaattg aggaggtgct tgcataatga acttactaga cgtaatcaaa cgcccagtga
    48481 tcactgaaaa atcaatgctt gccatggatg acaagaaata cacttttgaa gtggacactc
    48541 gcgcaaacaa aactttagta aaacaagcgg tagaagctgc ttttgacgtt aaagtcaaaa
    48601 acgtgaacat cgtgaacgta cgtgcgaaat tcaaacgtat gggcaaatat gcaggatata
    48661 caaaaaaacg tcgcaaagcg atcgttacat tgactgaaga ttcaaaagaa atccaattat
    48721 tcgaagctgc tgaataagca ctaatagata actcattaaa ttaggaggga aaaacgtggc
    48781 gattaaaaag tacaaaccta cctcaaatgg tcgtcgtaac atgacaggtt ctgattttgc
    48841 tgaaatcaca acatcaacac ctgaaaaatc attgttacaa ccattgaaaa accatgctgg
    48901 acgtaacaac aacggccgca tcactgtacg tcatcaaggt ggcggacaca aacgccaata
    48961 tcgcgtgatc gacttcaaac gtaacaaaga taacgttgtt gcaactgttc aaacgatcga
    49021 atatgatcca aaccgttcag ctaacatcgc gttagtacac tatgaagatg gagtgaaagc
    49081 ttacatctta gcacctaaag gactagaagt gggtatgaaa cttgtttcag gtccagaagc
    49141 agatatcaaa gtcggaaatg ctcttccatt agaaaacatt ccagttggta ctgttatcca
    49201 caacattgaa atgaaacctg gtaaaggtgg acaattgatc cgttctgctg gtacaagtgc
    49261 tcaagtactt ggtaaagaag gcaaatacgt attgatccgc ttgaactctg gtgaagttcg
    49321 tatgatctta gctgcatgcc gtgctacaat cggttctgta ggtaacgaac aacacgaatt
    49381 aatcaacatc ggtaaagctg gacgctctcg ttggatgcgt aaacgcccaa ctgtacgtgg
    49441 tagcgtaatg aaccctaatg atcacccaca cggtggtggt gaaggtaaag ctcctatcgg
    49501 acgtaaagca ccagtttctc cttggggtca accagctctt ggttacaaaa ctcgtaacaa
    49561 aaaagctaaa tcagacaaac ttattgttcg tcgtcgcaaa aataaataat tactttgacc
    49621 atgtgtcgca cattttgaga ggaggttcac catgggtcgt agtttaaaga aaggaccttt
    49681 tgtcgatgag catttaatga aaaaagttga agctcaagca ggggttgaaa agaaaaaagt
    49741 tatcaaaact tggtcacgcc gttcaacagt tttcccaagt tttgttggat ataccatcgc
    49801 agtttatgat ggacgtaaac atgtaccagt ttacatccaa gaagacatgg taggacacaa
    49861 attaggtgaa tttgcaccta caagaactta tcgtggacat gctgctgacg ataagaaaac
    49921 gaaacgctaa tttgagggga ggataaatcc aaatggcaga acaaatcaca tcagctaaag
    49981 caactgcaaa aacagttcgc acttcacctc gaaaagcccg tttagtaatt gaccttatca
    50041 gaggtaaaag tgtcgcagat gcaatttcaa tcttgaaatt cacaccaaac aaatctgctg
    50101 gaatcattga aaaagtattg atgtcagcag ttgcgaatgc agaaaataac tttgacttag
    50161 atgttgaaaa cttggtagta agcgaagctt ttgttaacga aggacctaca atgaaacgtt
    50221 tccgtcctcg tgcaaaagga tcagcttctc caatcaacaa acgtacaagt catatcacgg
    50281 tagtcgtatc agaaaaataa ggagggataa tcagtgggtc aaaaagtaca tccaattgga
    50341 atgcgtgtag gcatcatccg cgactgggat gctaaatggt atgctgaaaa agagtatgct
    50401 gagttcttac acgaagattt aagaatccgt aaatttattt caactaaact tgctgatgct
    50461 gctgtatcaa cagttgaaat cgaacgcgca acaggtcgcg taaacatttc aattcacaca
    50521 gctaaaccag gtatggttat tggtaaaggc ggttctgaag tcgaaagcct aagaaaagaa
    50581 ttaaacaaat taactggtaa aagaatccat atcaatatcg tggaaatcaa aaaaccagat
    50641 ctagatgcaa aattagtagg tgaaggaatc gcacgtcaat tagaaaaccg tgttgctttc
    50701 cgtcgcgctc aaaaacaagc catccaacgc tcaatgcgtg ctggcgctca agggatcaaa
    50761 actcaagtat ctggtcgtct aaacggagca gatatcgctc gttcagaagg atactcagaa
    50821 ggaactgttc ctcttcatac tttacgtgcg gacattgatt acgcatggga agaagcagat
    50881 acaacatacg gaaaactagg agttaaagtg tggatctatc gtggagaaat tcttccaact
    50941 aaaaaaaaca ctgagaaagg agggaaataa tcatgttagt acctaaacgt gtaaaacacc
    51001 gtcgtgaatt ccgcggaaaa atgcgcgggg aagctaaagg cggaaaagaa gtagcattcg
    51061 gtgaatacgg tttgcaagct gttgattcac attggatcac aaaccgccaa atcgaagctg
    51121 ctcgtatcgc aatgactcgt tacatgaaac gtggtgggaa agtatggatt aaaattttcc
    51181 ctcacaaatc ttatactgcc aaagcaattg gggtacgtat gggttctggt aaaggggcac
    51241 ctgaaggatg ggttgcacca gtaaaacgtg gtaaaatcat gtttgaaatc gcaggcgttc
    51301 ctgaagaagt agctcgtgaa gcgttacgtc tagcttctca caaattacca atgaaaacta
    51361 agatcgtaaa acgtgaggaa atgggtggtg aatcgaatga aggttaaaga aatcagagaa
    51421 ttaaccactg ccgaaatgct tgatcaagaa aaacaattaa aagaagaatt gtttaatctt
    51481 agattccaat tagcaacagg tcaattagaa aacactgcac gtattaaaga agtacgtaaa
    51541 tcgattgcac gcatcaaaac tgtgttgcgt gaacaagcta agtaattatg gaaggaggcc
    51601 attaagcgta tgactgaagg aagaaatcaa cgcaaagttt atcaaggtcg cgtggtttca
    51661 gacaaaatgg ataaaacaat taccgttgct gtcgaaacaa agaaaaacca ccctatctat
    51721 ggcaaacgca tgaactattc taagaaatac aaagtgcatg atgaaaacaa cacagcaaaa
    51781 gttggcgaca tcgtgagaat tatggaaact cgtccattat cagctacaaa acgtttccgt
    51841 ttgttggaag tagtcgaaga agcagttatt atctaataac acgagatttt cctatataga
    51901 ttgaatgcaa aatctgaaag gaggatacac aacgtgatcc aagcagaaag tcgtttaaaa
    51961 gtcgctgata actcaggcgc acgtgaaatt ttgacgatca aagtccttgg tggatctggt
    52021 cgtaaaactg ctaacatcgg tgatgtgatc gttgctacgg ttaaacaagc aacgccaggt
    52081 ggggttgtca aaaaaggtga cgtcgttaaa gccgttatcg ttcgaacaaa atcaggagct
    52141 cgtcgtacag acggttcata tattaaattt gatgaaaatg ctgcagtgat tattcgtgat
    52201 gataaaagcc ctcgtggaac acgtatcttt gggccagttg cacgtgaact acgtgaaaac
    52261 aacttcatga agatcgtttc actagcacca gaagttttat aatcttgaga cacttatcaa
    52321 aggaggtgcg aaacagttat gtttgttaaa aaaggcgata aagttaaagt tatcactgga
    52381 aaagacaaaa acaaagaagg cgttgtacta gcagcgttcc cgaaaaaaga taaagttatt
    52441 gtcgaaggtg taaatgtcgt taaaaaacac caaaaacctt ctcaagctgc tcctcaaggt
    52501 ggaattgttg agatggaagc gccaattcat gtttctaacg tgatggtggt tgactctact
    52561 ggtgtagctg gccgagttgg ctacaaagaa gtagacggca aaaaagtccg tgtttctaaa
    52621 aaaaccggtg aagttttaga taaataaaag tttaaggaag gagggtctta ctgaatgaac
    52681 cgcctaaaag aaaaatatct taaagaagta actccatcat tgatggaaaa atttaactat
    52741 agctctgtta tgcaaacacc taaagttgaa aaaatcgtca tcaacatggg tgttggtgat
    52801 gcggtatcaa acgctaaaaa cttagataaa gcagttgaag aattaacttt gatcactggt
    52861 caaaaaccaa tgattacaaa agctaaaaaa tcaatcgctg gattccgttt acgtgaagga
    52921 atgccaattg gtgcaaaagt tacattgcgc ggagaaagaa tgtacgaatt cttagacaaa
    52981 ttggtttctg tttcactacc acgtgtacgt gacttccatg gtgtaagtaa aaaagccttt
    53041 gacggtcgtg gaaactatac tttaggtatt aaagaacaat tgatcttccc agaagtagat
    53101 tatgatttag tagataaagt acgtggtatg gacatcgtta ttgtaacaac agcaaactct
    53161 gatgaagaat ctcgtgagtt gttgacacaa ttaggcatgc cattccaaaa ataattaaag
    53221 gaggcgaact acgtggctaa aaaatcaatg attgctaaaa acaaacgtcc tgcaaaattt
    53281 tcaacacaag cttatactcg ttgcgaacgt tgcggacgtc cacattcagt ttatcgtaag
    53341 ttccatcttt gccgtatttg cttccgcgaa cttgcctata aaggtcaaat tcccggcgtg
    53401 aagaaagcta gctggtaaaa agtaaattcg cataagaagg aggtaaaaga tcaatggtca
    53461 tgacagatcc aattgcagat tttctaactc gtatccgtaa cgcaaacatg gttaaacatg
    53521 agtctttaga agtacctgca tcaaaaatta aacgtgacat cgctgaaatc ttgaaacgtg
    53581 aaggtttcgt tcgtgatgtt gaatacatcg aagatgacaa acaaggcgtg atccgtgttt
    53641 tcctaaaatt tggtaaaaac ggcgaacgtg ttatcacaaa cttaaaacgt atctctaaac
    53701 caggtttgcg tgcttatgtc aaagctgacg aagtaccaaa agtattgaac ggtttaggta
    53761 tcgcaatcat ttcgacttct gaaggtgttg tcactgataa agaagctcgt gaaaaaaata
    53821 tcggcggcga agttatcgct tacgtatggt aatcaagtag aatctacaag gaggtgtctt
    53881 taagtgagcc gtatcggaaa taaagtcgtt gttatccctg aaggagtaac agttactcaa
    53941 gacggaaaca acgttacagt taaaggacca aaaggtgaat tgactcgtac tttttcagca
    54001 gatatcaaaa tgaatatcga aggaaacgaa gtaactttca ctcgtccaaa cgacagtaaa
    54061 gaaatgaaaa caattcatgg aacaactcgt gcgaacttca acaacatggt tgtaggtgtc
    54121 agcgaaggtt tccaaaaagc gttagaacta atcggggttg ggtaccgtgc acaattacaa
    54181 ggatcaaaac ttgtattgaa cgttggttac tcacatccag tagaaatcac accaccagct
    54241 ggagtaactg ttgaagtacc agctaacaca caagtaattg ttaaaggtgc gaacaaagaa
    54301 gaagttggcg aattagctgc aaacattcgt ggaactcgtc ctccagaacc ttataaaggt
    54361 aaaggtattc gttatgttgg cgaatatgta cgccgtaaag aaggtaaaac tggtaaataa
    54421 tcgtttagaa aaattgcata gaagccagca gcaggaaaaa ggcatttggc tgaagtttac
    54481 cttcaaaaaa atacattttt acttgtgccg cagcttctat ttttcgaaac gtatttactg
    54541 cttgaaatag tagtacaata aaaataaaga ggtgacaatt gtgattacaa aaccagataa
    54601 gaacaaaaca cgtcaaaaga gacatcgtcg tgtacgtaac aatatctctg gtactgctga
    54661 gcgcccacgc ttgaacgttt ttcgttctaa caaaaacatc tacgctcaaa ttattgatga
    54721 cgtagcgggt gtaacgctag caagtgcctc taccttggat aaagaaattt caggtggaac
    54781 aaaaacagaa gcagcacaag ctgtcggtaa actagttgct gaacgtgcag ctgaaaaagg
    54841 cattaaagta gtagtctttg accgtggtgg atacctttac catggccgtg tgcaagcttt
    54901 agctgaagct gctcgcgaaa atggactaga attttagaag aaggaggaat accattcatg
    54961 gtttatatcg atccaaaaca tttggaatta gaagaccgcg ttgttgcaat taaccgtgta
    55021 acaaaagttg ttaaaggtgg acgtcgtcta cgttttgctg ctttagttgt tgtcggtgac
    55081 aaaaacggac acgtagggtt tggtacaggt aaagctcaag aagttcctga agcaatccgt
    55141 aaagcaattg aagatgcgaa gaaaaactta gttgaagtac ctatggttgg ttcaacaatc
    55201 ccacacgaag taatcggtgc ttttagtggt ggtagaatcc taatgaaacc tgctgtagaa
    55261 ggttctgggg ttgctgctgg tggaccagtt cgtgccgtat tagaattagc tggggtagca
    55321 gatatcactt ctaaatcttt aggctcaaat acaccaatca acgttgttcg cgcaacagtt
    55381 gaaggtttga aacaattgaa acgtgcagaa gaagttgcag aacttcgcgg caaatcagtt
    55441 gaagaattaa tcggataagg aggacaacaa taatggctga attaaaagtt actttaaaac
    55501 gcagtattat cggacgtcct caaaaccaac gcgatacagt taaagcgtta ggtcttacaa
    55561 agattaacag ttcagtggtt aagccttcta atgaagcaat taaaggcatg atcaatactg
    55621 tttctcacct agtggacgtt gaagaaattt aatttagaaa aatcataact agaacattaa
    55681 ggaggtgcca aacctatgaa acttcatgaa ttaaaacctg ctgaaggttc acgccaagta
    55741 cgtaaccgtg ttggtcgtgg tacttcatct ggtaacggaa aaactgccgg acgcggacaa
    55801 aaaggacaaa aagctcgttc aggcggcggt gtgcgtttag gatttgaagg gggacaaaca
    55861 ccattgttcc gtcgtttacc aaaacgagga ttcacaaacg tgaaccgtaa agactatgca
    55921 gtcattaatc ttgatgtatt gaaccgcttt gaagacggag ctgaagtaac tcctgttgct
    55981 ttagttgaag ctggaatcgt gaaaaacgaa aaagctggaa tcaaagtatt agctaatggg
    56041 gaactaaaca agaaattaac tgtgaaagca gcaaaattct ctaaagcagc tcaagaagca
    56101 attgaagcgg ctggtggatc aatcgaggtg atctaatgtt tagactatta aaagatgctt
    56161 ttaaagtaaa agacattaga tcgaaaatcc tatttactgt atttgtgttg tttgtatttc
    56221 gcctaggtgc acatattact gtaccaggcg taaatgctgc ttcattgcaa aacctttctt
    56281 ctttgccatt tttcaacatg ttgaacttgg taagtggtag tgcgatgcaa aacttttcca
    56341 ttttctcaat gggggtttca ccatacatta ctgcttctat cgtcattcag cttcttcaaa
    56401 tggacatcgt gcctcgattt gttgaatggt cgaaacaagg ggaagttggt cgtaaaaaac
    56461 tgaatcaagc gactcgatat ctgacattag tattggcatt tatccaatct gtcggtatca
    56521 ctgctggatt caatcaatac gctcaattag gtttcgtgaa taatcctaac gtagcaactt
    56581 acgtaacgat cggtttgatt ctaacagccg gtacgatgtt tgttacttgg ctaggagaac
    56641 agatcacaga aaaaggtatc ggaaatggcg tatcaatgat catttttgca ggtatcattt
    56701 ctagattgcc tgaaagtatc agagaaattt atgaagatta ctttataaat attgatcctt
    56761 cagacatatg gaaatctgct atttttgtgg ccattttact agtagcggtc ttgttgatcg
    56821 tgacattcgt aacatttttc caacaagctg aacgcaagat cccgatccaa tatacgaaac
    56881 gcgtagctgg cgcgcctaca agcagctatc tgccgttgaa agtaaatgca gctggagtta
    56941 ttccagttat ctttgctagt tcgtttattg ctacgccaaa tgctgtttta caagcattga
    57001 gcggtagatt cggttctgaa aactggtaca aagtgatgat ggagatcttt aactataata
    57061 cagcacctgg tgctacgatt tatacgattc tgatcgttgc cttcactttc ttctacgcct
    57121 ttgttcaagt aaaccctgag aaattagcgg aaaacttgca aaagcaagga agttatatac
    57181 caagcgtacg ccctggtaag gggactgaag agtatgtctc aagattactg atgcgtttaa
    57241 gtacagttgg tgcagtcttc cttggtttag tggcattgct tccaatcatt gctcaaatgg
    57301 tttgggatct tccacaatca atcggattag gcggtacaag cttactgatc gttatcggtg
    57361 tagctttaga aactgcgaaa cagttagaag gcttaatgct gaaacgtaaa tatgttggat
    57421 tcattgagta agaaagcgtg ttgaggtttc ccctcaacac acgttctcac acatttatta
    57481 ggagggaaaa caatgaacct cattttaatg gggctgccag gcgcaggtaa aggaacacaa
    57541 gctgaaaaaa tcattgatgc ttatggtatt ccgcacatct caacaggaga tatgttccgc
    57601 gcagcgatgc aaaacgaaac agctcttggc ctggaagcaa agtcttatat ggataaaggt
    57661 gcattagttc ctgatgaagt aacaaatgga atcgtgaaag agcgactagc tgaaccagat
    57721 acagagaaag gcttcttatt agatggcttt ccacgtacgc tagatcaagc aaaagctctg
    57781 gacgcaatgc taaaagattt gaataaaaaa attgatgctg tcatcgatat ccatgttggt
    57841 gaagaaatct tggtcgaacg tctagccggc cgatttatct gcagcaattg tggagcaact
    57901 taccataaaa ttttcaaccc tacaaaagtt gaagacacat gtgatcgttg cggtgggcat
    57961 gaattctatc aaagagaaga tgataaacct gagacggtta aaaatcgtct ggctatcaac
    58021 atcaaaaata gtgaaccaat cttggcttat tataaagaac aaggtttgct aaacactatt
    58081 gatggtgctc gtgaaatcga tgctgtattt gcagatgtac aaaaaatcat tgaaaaatag
    58141 cacgattgcc cgtagccgac ttgcattatg cagttactct tgtcaagttc aataaattat
    58201 gatagaatat gtcagtccga cttctagaga atcctctaga tgatccattt tgagaatttt
    58261 gaggtgggaa ctgcagtttc cgccatttta tcgtgtgaaa atgcgaaaca agtacaagga
    58321 ggtactgtgc gtggcaaaag aagatatgat tgaagtcgaa ggtacagtcg tcgaaacttt
    58381 gccgaatgca atgtttaaag ttgaactaga aaacggacac caagttcttg ctactgtttc
    58441 aggtaaaatt cgtatgcatt acatccgtat cttacctggt gacaaagtaa cggttgaatt
    58501 gtctccatac gatcttaacc gtggccgtat tacttatcgc tttaaataat tgtactccgt
    58561 aaaactacag gaggtattat catgaaagta agaccatcag taaaaccaat gtgcgaacat
    58621 tgtaaagtaa ttcgtcgtaa aggacgcgtt atggtgattt gcccagcaaa tccaaaacat
    58681 aaacaacgtc aaggataatt ggaggtgcta taaatatggc tcgtattgca ggagtagata
    58741 ttcctcgtga taaacgtgta gtagtttcac ttacttatat ctatggtatt ggtaacacta
    58801 ctgctaaaaa gatcttagct gatgctggtg tatctgaaga tgttcgcgtt cgtgaattaa
    58861 cgaatgaaca aacagatgct atccgtgcag aagtagataa attaaaagtt gaaggggatc
    58921 ttcgtcgtga agtgaacttg aacatcaaac gtttgatgga aatcggttct taccgtggaa
    58981 tccgtcatcg ccgtggattg cctgttcgtg gacaaaacac gaaaaacaat gcacgtactc
    59041 gtaaaggccc agctcgcgct atcgctggca agaaaaaata attcttaagt gaaggaggtt
    59101 aaaacttcat ggcagcaaaa aaagtgaatc gtaaacgccg tgtgaagaag aatatagaag
    59161 caggtattgc acatatccat tctacattca ataacactat catcatgatc actgatactc
    59221 acggaaatgc attagcgtgg tcatcagctg gttcattagg tttcaaagga agcaaaaaat
    59281 caactccttt tgccgctcaa atggcagcag aaactgctac aaaagcagca atggaacacg
    59341 gattaaagac tgttgaagta acagttaaag gacctggttc tggacgtgaa gcagcaattc
    59401 gttcattaca agctacaggt ttagaagtga ctgcaattcg tgacgtgact ccagttcctc
    59461 ataatggtgc ccgccctcca aaacgccgtc gtgtttaatg agtgccgctc attttgagtt
    59521 ttatcagtaa cgtcattgaa caagacactt ttcgttttga aaggggtaaa gataagaatg
    59581 attgagttcg aaaaaccaag aatcgcaaaa attgatgaag aaaaagatta tggcaagttc
    59641 atcgttgaac ctcttgaaag agggtatggg actactttag ggaattccct acgtcgtatt
    59701 ttattatctt ctttgccagg tgctgccatc actaatattc aaatcgatgg tgtactgcat
    59761 gaattctcta ccatcaaagg tgttagagaa gacgtcactc aaatcatctt gaacattaaa
    59821 ggcttagcgc tgaaaatgta tggccaagaa gaaaaaaccc ttgaaatcga tattaccgga
    59881 cctgctacag taactgctgg cgatattatc gttgatagtg atgtagaaat tcttaacaaa
    59941 gatatgtaca tctgtagtgt ctctgaagga gctacgttcc atgctcgctt aacagtgaaa
    60001 cctggacgtg gttatgttca agcagatgaa aataaaaaag aagatatgcc aattggcgtt
    60061 cttccagtcg attcaatcta cacacctgtt cgtcgtgtga actatcaagt tgagaacaca
    60121 cgtgttggac accgtgaaga ttttgataaa ttgacgatgg agatttggac agatggttca
    60181 attgaaccgt tggaagcaat gagtttgtct gcgaaaatca tgactgaaca tttggatatt
    60241 tttgtgaact taactgatga agcaaaaaat gctgaaatca tggtcgaaaa agaagaaaca
    60301 caaaaagaaa aaatgttgga aatgacgatc gaagagttag acttgtctgt tcgctcttac
    60361 aattgtttga aacgtgcagg aatcaatact gtacaagaac ttacaaataa atctgagcct
    60421 gaaatgatca aagtccgtaa cttaggacgt aaatcattag aagaagttaa agcaaaattg
    60481 cacgacttag gtttaggttt acgtaaagaa gattaaaacc ttttacgaag gagggaaacc
    60541 aacgtgagtt atcgtaaact aggacgcaca tctagccaac gtaaagcgat gttgcgtgat
    60601 ttaacaactg atttattaat caacgaacgt attgtgacta ctgaagctcg tgcgaaagaa
    60661 gttcgctcaa ctgcagaaaa aatgatcact ttaggtaaac gcggagattt gcatgcacgt
    60721 cgtcaagctg cggctttcgt acgtaatgaa gttgccagcg tgcgtgaaga aaatgaggac
    60781 atcgtcattg aatcagcttt acaaaaactg ttcaacgata ttgcacctcg ttacgcagaa
    60841 cgtcaaggcg gatacacacg cattttgaag acagaaccaa gacgcggtga tggtgcacca
    60901 atggttgtct tagaacttgt ctaaaatcac actaataaca tcactttata gatgattctt
    60961 ttagagtgtt atgatgttgg aagatatttt tccgagtcta gctcagcgct gctcctcaag
    61021 cgaatatcgc ttggggagta agcactcatc ataaagtgtt tttttatacc ttcaattacc
    61081 tgtcatgaca gatatgtttg aaggcataaa aaaacatttt aagcgagaaa taattgaaaa
    61141 ttttgtcttt ttttgatagt gttagtagga actttaggat gaagaaggag taatcatgga
    61201 gccaattatt gaattagaaa agataaatta taaataccag ccagatgatc ttcgccccgc
    61261 attgaaagat gtctccttta caattgataa aggtgagtgg atcgctatta ttggacacaa
    61321 tggttcagga aaatcgacac ttgccaaaac gatcaatgga ttgcttctgc cagaatcagg
    61381 gatcgtgaaa gtgggaaatc aaatattgga tgaagaaaat atttggacga tccgacagat
    61441 ggttggaatg atttttcaaa atccagacaa tcagtttgtt ggttcgacag tagaagatga
    61501 cgttgctttt ggtcttgaaa atcaagggat tcccagagaa gaaatgctag tgcgtgtgaa
    61561 agatgcactt gagaaagtta gaatggctga gtttgcttca cgcgagcctg ctcgtttatc
    61621 aggagggcaa aaacaacgtg ttgccattgc cggcgtggtt gccttacgac ctgatatcat
    61681 tattttagat gaagcaacca gtatgctaga tccagaaggt cgtgaagaag tgatctctac
    61741 gatcaagaag atcaaagaag aaagtcaatt gaccgttata tctatcactc atgatattga
    61801 tgaagcagct aatgccaatc ggatactagt gatgagacaa ggcgaacttg tccgtgaagg
    61861 tacaccaaaa gaaatttttt ctgctggtcc tgaactgatc gacttaggtc tagatctacc
    61921 atttcctgaa aaactgaaga gcgcactgaa agaacgtggg gttgatgtgc ccagtgaata
    61981 tatgacagaa gaaaggatgg tggattggtt atggacatcc gttttgaaca agtagacttc
    62041 acttatcagc cgaatacacc atttgaacaa agagccttat ttgacatcaa tatgacgatc
    62101 aaagaaaaca gttatactgc attagtaggg cataccggaa gcgggaaatc aaccttactt
    62161 cagcatttga atgcgcttgt taagccaaca agcggtacag tccatattgg agaacgagat
    62221 attcagccgg atacggataa taagaattta aagcccatcc gaaaaaaagt tggcatcgtc
    62281 tttcagtttc cagaagcaca gctattcgaa gaaacggtag caaaagacat tgctttcggt
    62341 cctaaaaact ttggtgtcag cgaagaagaa gcactagtcc tagcaaaaga aacattagaa
    62401 caagttgggc tggatgaaag ctatttggaa cgttcgccat ttgaactttc aggagggcaa
    62461 atgcgtagag tagcaatcgc tggtgtgctt gccatgaggc cggaagtact tgtattggat
    62521 gaaccaacgg caggacttga tccccagggc agaaaagaaa tgatggagat gttttggcgt
    62581 cttcataagg aacagcagat cactattgta ttagttacgc atttaatgga tgatgtagcc
    62641 aattttgcag attatgtcta tgtgttggaa aagggacgaa tcgttaatag cggggagccg
    62701 caagaggtct ttcaaaacat tgaatggcta aaagaaaaac aactaggcgt acctactgcc
    62761 accgagtttg cagaagaatt aatggctaaa gggatgaact ttgctacctt gcctttgaca
    62821 gctgaagaat tagcagacgc tatcgtccat tatgctggag gaaatgccca tgatgaataa
    62881 attgatattt ggtcgttata tgcctggaga ttccttgatc catcgattgg atccccgagc
    62941 taaattatta gccagttttt attttatcgg aatcattttt ttagcaaata attgggaaac
    63001 gtaccttttg ttggcatttt tcacattact ttctgtcttc ttgtcaaaag tagatttgaa
    63061 attttttatc cgaggcattc gcccattgat ctggctaatc ctcttcactg tagctttgca
    63121 ggtcttgttt acaagtggcg gagaaactta ttggagttgg ggtgttttcc atatcactga
    63181 atttggtgtg atgaacggcc tctttatttt ctgtcgattc gtgttgatca tttttatgtc
    63241 gactctactc acgcttacta caccgccttt ggaattatcg gatgcgattg agtacttgct
    63301 tcgtccattg aaagccgtac ggttcccagt ccacgaaatt tcattgatgc tgtcgattgc
    63361 tcttcgtttt gtgcctacat tgatggacga aacagaaaag atcatgaacg ctcagcgcgc
    63421 tcgcggtgtt gattttggtg aaggaaacct agttcagaaa atgaaagcag tcattccttt
    63481 gctgattcct ttatttgtca gcagcttcaa ccgtgcggaa gatcttgcga ctgctatgga
    63541 agcaagaggc tacaaaggtg gggaaggaag aacgaaatat cgtgtattgc attggcataa
    63601 tagagacacg atggttatca ttgcttttgt attattaact gtgctactga tctttttaag
    63661 aggataaacg ttcagtgggc cgctatctat tcttttcttg ctgatctgtt tgcaagaaag
    63721 gatgcaagca gtgtatgaca agaaatatta gggtctgtgg cagactaagg tcacaggcct
    63781 ctttctttta tctgaaaaga aagaacgaaa aaattttata gaaaaatcat tattccagat
    63841 aaccttttag gaacaaagga gagaaaaaca tggtacgtta caaagcaatc attgcgtacg
    63901 acggaacaaa ttttaatgga ttccaaaaac agccaaatgg gcgtactgtt caagaagaag
    63961 tagaaaaaac attacaaaaa atggcaaatg gaaaagaaat cacggtcttt ggttcgggaa
    64021 ggacagatgc cggagtacat gcgatcgggc aagtaatcca ttttgattat ccagaggagc
    64081 gtccgttaga acgaatgcgt ttcgcgctag atacccagtc accagaggat attgctgtaa
    64141 gacaagtaga aattgtttca gaagacttcc acgcaagata tttggtcaaa gaaaagacgt
    64201 atcaatttcg agtggatata gggaaaccac gcagtccttt tcgacgccat tatgcaagtt
    64261 attttcctta cccacttgat ttggaaaaaa ttcagcgggc gcttcctgat ttattaggga
    64321 ctcacgattt cacctctttt tgtgcatcag gaagctcgat agaggataaa atacgtacga
    64381 tctacgaggc taagatggag gtaaatgaaa caaaagacga attgttgttt acatttaggg
    64441 gaaatggctt cttgtataag atgatacgga tcttagtagg aacgcttttg aaaattggca
    64501 atggccgatt gcctgaagat agtatcccag atattattgc gaaaaaagat cgtaatgcag
    64561 caggacctac agcacaccct gaaggacttt atttgtatga agtgaaatat gaataagagg
    64621 ttatgataga agtggtcagc ttcaagaaat aagaaggtat ttacgaaaat tacttctcaa
    64681 aattttgcaa aatttcggct tattttcgaa gaagctactt ctggaacacc gtttaagaaa
    64741 gaggtcgtga gcctgtcatt taactctgag tcacaaagag caatttcaaa aaacagcttc
    64801 tcatctttga tgaatatcgt gacttgtgga cgaggcgttg ataagtgaat catctttttg
    64861 ttacctattt tttctgtaac caaaaaacga tggaacgttg ttttgattgc ccttggtgcg
    64921 gacagtctga acaggttcca tcgtttcttt attttctacg tcttcttctg tgtgtgtttc
    64981 gtggttgttc ttctattgct ggatcaatcg tgccataaaa ggggcgattg cggtcaaggt
    65041 accagacata aagaataaac aatgcaatac ataggacaaa aaggattgca cctatcgtaa
    65101 atccttcagg caagtaactg aaagtgacag tatgtttacc tgctggaact tttatcatca
    65161 agaatgcgtc tttgaaattg ctgaccttga ctggtttccc atcaatttta gctttccatc
    65221 ctttgtcata aggaatagtt gtcaccaaca tggaatcttc agcggtattt acagtcccag
    65281 aagcttttct gccagtggct tccatttcga cacctttttc tttgatctga ttcattgcgg
    65341 cttgataggc tacaacatct agaccaacga ctttaggctc catgaaactt atttctttcg
    65401 tcccataaaa acttgcagta aatgtcactg ttgtctcttg aggataatag ccaagatcat
    65461 agtattgacc agaaatgttg atttgcgttt ttcgcgatgt accattgaca gtgatcgtcg
    65521 cactagaact ttctaattgc gcaaaattgg ttgggaacaa acttaagtaa gcttgtgtat
    65581 ttgcaggcac atggacttcc caagtgagct cttttggctg gttatctgct gtttctgagt
    65641 aagtgacgcc agtcgttgtc tttgtgatcg tcgtattcaa ctgattcttc aaagtaggtg
    65701 tatagaatgt aaaatactgt tcatttagac cagacaatgt attgatcaag tttgtctgac
    65761 tggtcaaatt atctgtcact ggctgctcaa catcattaac ggacaacggt gcagtaaatg
    65821 ctagtggcaa tgcattgaca ttctgataca gtgtatagtc gccgctttga ccagcttggc
    65881 ggaagccata tttactgaac gtgctttcgt ttttagaaat gttatatttg attcctgtaa
    65941 acccatccat cagcaaagta ttattgttat aacggatatt caaatttgtc cctcttgaac
    66001 ggaaacctaa tttatccagg taagaggaag aattacgatt tcttatagaa gaaaacaaac
    66061 tgataccact gtaaccatag ttgatcgaat cgtttgcaga tactggatcc agattttcca
    66121 agcggtaaaa cgagtcggaa tctttttttg tttcatttac aagagctttc aagtcaggat
    66181 aaggatcagt atataaactg cgggaagcat aattccaatc atcaagaatg ccatggatca
    66241 tactgttcgt attgaccagt gcttcagcag acatgatcaa taataacaat atactcagat
    66301 gattgaatga aagtttttta tgctgataaa atcccacagc aaaagcataa atccctaaga
    66361 aaaccactgt caatacaaag gaagtcatcg tcacataggt gtagcttcct tcaggtttga
    66421 tgccccatgc caccgcaaag agagcagcta cgaccaaaat gattcctaac aacagcccac
    66481 catcgtcttg ttctatcttt tcccatcctt ttcctgcaag caggagcact aagaaagaga
    66541 acagataggc ataacggaat aaaaacatat tcggtgcatg cataccatgc cagaacaaat
    66601 tcaatggaac aagataaaaa cttgcgataa gtatggcgaa aagactgccg tacaaaaatt
    66661 tatttttcaa cggtactttc cgactggcaa aataaaacag acagaggact aatggtagca
    66721 gacctgcgta aacgaacgga atcgatccgt attttgtcgt gtcataaaca ccaatcatat
    66781 ttttcatgat tagatccaga taagaagtcg cttctgtttt caacgtagtg atctgactca
    66841 gtgcctcgcc atttgaccga agatccagca ctgctggcaa tacaatgatc attgaagcgc
    66901 cgcctgccag taaagaagtg ataccataag gaacaatcgt ttttttatat tctgaccagt
    66961 ttgtcaatag acgggcgata aaatataaga aagaaaatac gccaatcata aaacccatat
    67021 aaaaactgga taaaaacaag agtaaataac tgataaataa aagtttcggc cgtttaagat
    67081 ccataacacg atgaatcccc aaaatcacta atggaagata aacaaatgca tccagccaca
    67141 tgatgatctc cgaatgtgca gtgataaatg agatcaatgc gtaactgacg ctaagtgcaa
    67201 catgccccca gcgaggaatc ttgaacgtac gtgcaagaaa ccaaaaagcc aatccggcac
    67261 agccgacctt aagcaaagtc agaacataca gtgcatctgg catcatctgg tttggaaaaa
    67321 agaacaccag tggcgtgaag atcccgccta gatagtaaga aattaaagat aaataattca
    67381 atcctagaga agcattccat gtataaaaaa tactttgctt tccaagaaga acatttcgga
    67441 agctggcatg aaaattagaa aactgtgaaa aggcatcact tgccaaaata cttctgctgc
    67501 ttccaggata gattccgata cttagataaa caagtgctaa aatcaaaaac ggaatcagga
    67561 aactagctgc tgcgtagcgt ccgttttctc taaagaatga ctttaaattt tttttcatat
    67621 ttattttcca ccaaatttct atgaagttgt attcagttta tcatatcctt gtttttctct
    67681 gcttaaaaag agtctagttt tcacaaaaaa ataaaattta gaataaataa cttcataatt
    67741 tgttcgtata acaaaaataa acgcaaaaaa agggtaggaa tcaaaagtcc cacccttaaa
    67801 aatttttatt aaataaaaaa ccgttcacta tctgaacggt ccaagtatca ttcaattaaa
    67861 cgcgttcaac ttttcctgat ttcaaagcac gagttgacac ccaaactttt ttaggttttc
    67921 cgtcgaccat aacgcgaact ttttgtaagt taggtttaac agtacgtttt gttgagttca
    67981 tagcgtgtga acggttgttt ccactttttg tcttgcgacc tgtaaagtaa caaacttttg
    68041 ccatggtaat ttcctccttt gccttgattt ggagcaatag aataattcag ctcataccag
    68101 actaacatat catatagaat gggaatttgc aagtacattt tcatagatac aattgacaat
    68161 tgagaagaat aattgtaaat taaacaaggt tatacctgaa attttgcaag gtttttgagt
    68221 actttgggta actgcctttt atttcaagtt gcgctatgat aaaatatgat agaagaaaaa
    68281 tgggagtttt ttacccgtta aggaggctca ttcatggctg taaaaattca aacgtcagct
    68341 ggaaccattg aaattaccaa tgatgtaatt gcaacagttg ttggtggcgc cgcgacagat
    68401 gtgttcggtg ttgtaggaat ggccagcaaa aatcaaatta aagataatat tactgagatt
    68461 ttgcgtaaag aaaattactc aagaggagta gttgtccgcc aagaagataa tggtattgct
    68521 gtagatgttt atacgattat cagctatggt acgaaaatct ctgaagtttc ccgcaacgtc
    68581 caagaaaaag tgaaatacaa tctagaggca ttattaggcg taactgccaa ttcagttaat
    68641 gtctttgtac aaggtgttcg tgtactgcca gactaggcag gagactggct ggtaactcag
    68701 cagtcgatct gaaggaggat tatctaggtg agtgtaacag aaataagtgc aagccagttc
    68761 caggaaatgg tccaggcagg tgcgaatcgt ctgcaagtaa atgcagaata cgtcaattca
    68821 ttaaatgtat tccctgtgcc agatggcgat acgggaacaa atatgaatct atctatgact
    68881 agtggagcta aggcagtagt agattcaacc tcagaaaaag taggagaatt agcagcaatg
    68941 ctttcaaaag gattgctgat gggtgcgcgc ggaaattccg gcgttatttt gtctcaattg
    69001 tttcgtggat tttctaagca aattccagat gttgtctcat taaatgcttc tgatcttgct
    69061 gcagccttca ctcatggagt tgaaactgcg tataaagcag taatgaaacc agtagaagga
    69121 acgattttga cagtagctag agaagcagcg aaagctggag aaaagaaagc aaaaagtact
    69181 gatgatgtcg ttgaagtaat gacagctgtt gtaaaaggag caaagcgtgc tttagctaaa
    69241 actccagact tgcttccagt cctaaaagaa gtcggtgtcg tagacagtgg agggcaagga
    69301 ctgctgtttg tttatgaggg ctttttaagt gcattgaatg gagaattcca agcggatgaa
    69361 acatatgaac cttctccagc agaaatggat gatatggtca atgcagaaca tcatcgcagt
    69421 gtccaaggtc aaatggcgac agaagatatc cattttggtt actgtacaga aatcatggta
    69481 aaaatcggag aaggacctac agtcgatagt acattcgatt acgaagaatt ccgtaactat
    69541 ttagatggta taggagattc tttacttgtg gtcaatgatg acgagatcat caaagtccac
    69601 gtgcatacag aaaacccagg agaagtcttg aattatggac aaaaattcgg ttcattagtc
    69661 aaaatcaaag tcgacaacat gcgcttgcag catgagacga ttttggaaca cgatgaagaa
    69721 gtgaacgagt ttgcgaaaaa agaaatacca cgtatcccgt atggtatcgt agcgatcgct
    69781 gctggtaaag gcgtccaaga gctgtttgaa agtttaggcg ctaattatgt tatcagtggt
    69841 ggacaaacaa tgaatccaag tacggaagac attgttaaag cgattcaaga agtaaacgca
    69901 gataaagtga ttgtcttgcc aaacaataag aacatcttca tggcagctga tcaagcagca
    69961 gaagtagcag atatcccagt agcagttgtt ccttcaagaa cgatttccca aggattgaca
    70021 gctatgcttt cattcaatga tcaagcttct ttagaagaaa acaaagaaac gatgacagaa
    70081 atgttgtcta gcgttgtgag cggtcaagtc actcacgcga tcagagatac ctcaattgac
    70141 ggtgtagaga tcaaagaagg agactttttg ggcatgatcg atggaaaaat cctgatctct
    70201 aacccagata tcacagcaac agcgttaagt acactagaac aaatgatcaa tgaggacaca
    70261 gagatcgtaa cgatcttggc aggtgatgaa ggaacagttg aacaagcaaa tgaatttgct
    70321 gagaaactaa cagaaaaaca tcctgatttg gaaatcgaga tccaccaagg agatcagcct
    70381 gtctatcctt atctattttc tgctgaataa tttctgtgtc agaatatctt tctaacacag
    70441 tattcattca taaaaggcct tcggttagtg atcaggtatg atcgctgacc aagtcctttt
    70501 tttgttaaag gagggaggaa aaatttatgc gttctttaga tgatccaatc actatgctgg
    70561 caggagtcgg agaaaaaaga gcagctagtc ttgccagctt gggcatccaa acgattgaag
    70621 acttactgac ttattatcct tttcgctacg aagatattca ggaaagaaat ctaaacgaga
    70681 tccaggatca agaaaaggtt actttgaaag gaatcgtagt ctcgcctcct gtgatgaatc
    70741 gatttggtta taaaaaaagc cgattgcaat ttcgtatgat gcaagaacat gatgtgttca
    70801 acgtttcttt ttttaatcaa ccttatctaa aagaaaaagt cattttatcg gaggaaatcg
    70861 ccgtatacgg taaatgggat gcaaagcgta aagccttgaa cgggatgaag attttaggtt
    70921 ctcaaacaat ggatgacttt tcgccgatat atcacgtcaa caaatcaatt cgtcaaacga
    70981 cattagttga tctaatcaaa aaagggtttc aagaatacgg cacattgatt gaagaaaatc
    71041 tgccaaacaa ccttgtggag aaatatcgac tgcttgaccg agcttctgct gtaagaagta
    71101 tgcattttcc aaaagaccac gaagaaaacc atcaagccaa acgacgtgtc gtttttgaag
    71161 agttcttttt atttcaaatg cgtcttcaag gattgaaaaa agcagaaaaa gcagaaacga
    71221 atggtttgga gatcctttat gatgtccaaa aattgaaaca atttactcaa aagctacctt
    71281 ttgaactgac aaatgcacaa aaacgtgtaa cgaatgaaat ctgtcgggat cttcgcagtc
    71341 cgcagcatat gcagcgactt ttgcagggag atgtaggtag cggaaaaaca gtggttgccg
    71401 cgattgcgtt atacgctacc gttacagcag gtttccaagg tgcgttgatg gtgcctacag
    71461 aaatcttggc acaacaacat atggaaagct tgcaacagct gtttgatccc aatgaagtaa
    71521 ccatcgcatt gattacgggg tcaacgaaag caaaagaacg acgtgagctc cttgaaaaac
    71581 tagagcaagg ggaaattaac gtcgtgatcg gcacacatgc attgatccaa gatggagtcg
    71641 aatttcagaa tttaggtcta gttattacgg atgagcagca ccgttttggc gtgaaccagc
    71701 ggaaagtact tcgtgaaaaa ggctggcgac cagatgtctt atttatgaca gcgactccga
    71761 ttcctagaac attagctatt acagcatatg gtgagatgga tgtatctatc attgatgaac
    71821 ttccagctgg acgtatcccg atcgaaacac gctgggtaag aacacctcaa ttggacagtg
    71881 tactggattg gacgaaaaaa gaactggcaa gaggacatca aatgtatgtc atctgtccgc
    71941 tgattgaaga atcagaagca ttagatgtaa aaaatgcagt ggaaatctat gaaaaacttc
    72001 gtgaactatt tgcaccaaaa tatgaagttg gcttgttaca tggtaaaatg aagaatcagg
    72061 aaaaagaagt aatcatggaa gccttcaaag acaaccagat gcagatatta gtttcgacta
    72121 ctgtaattga agtaggggta aacgtgccga acgcgaccgt catgctgatc atggatgcgg
    72181 atcgatttgg attggcacag ctgcaccaac ttcgtggacg tgttggacgc ggaagtgatg
    72241 cttcctactg tatcttagtt gctaatccta aaaatgaact aggtgtggaa cggatgaaaa
    72301 tcatgacaga gaccaccaat ggttttgtac taagcgaaaa agatctggaa ttacgtggtc
    72361 ctggtgaagt atttggcttc cgtcaatctg gtctgcctca atttgcgatt gctgacttgg
    72421 tgacggatgg aaatgttttg gaggtagcca gagaagaggc tgcagccctt tggcaaataa
    72481 aggattggca gatgcttcct gagtatcagc ctttagcgca aagcctccaa atagaaaatc
    72541 aggataatcg ttatttcgat tagcagactg tgctatactt tatactgatg acggaaaaag
    72601 gagtggaaac aatgaaaatt gccgtagatg caatgggcgg agatcacgca cctcaagcaa
    72661 ttgtagaagg cgtggcttta gcgcagcaag attttccaga agtagaattc ttactttacg
    72721 gaaaagaagc agaaatcaag aaatacttaa caaatgaaaa aaacatcacg attatccata
    72781 cagacgaaaa aatcaatagt gatgatgagc cagtaaaagc aattcgacga aaaaagactg
    72841 catcaatggt ccttgctgca caagcagtga aaaacggtga agcagatgct gtattttcag
    72901 cagggaatac aggcgcgcta ctagcagcag ggttgttcat cgtaggacgt atcaaaaata
    72961 tcgaacgccc tggcttgatg tctaccttgc cagtagtagg gaaagaaggt gccggtttcg
    73021 atatgctgga tcttggcgct aacgcagaaa acaaaccaga acatttgttg caatacggta
    73081 ttttaggttc tttttatgca cgtaaagtcc gaggtatcgg acgtcctcgt gtcgcactct
    73141 taaacaacgg aacagaagca acaaaaggta gtgaagtgac gaaaaaagcc tatgaactcc
    73201 ttcagaatga aacaagcttg aattttattg gaaacgtaga agctcgtgag ctactcaatg
    73261 gtgtggctga tgtagtggtc acagatggat ttacaggcaa cgctgttttg aaatctattg
    73321 aaggaacagc aatgaatatg atgaatctat taaaatctgc tattctaaat gaagggatca
    73381 aaggaaaaat gggcgctatg cttttaaaag acggcttacg ttctttgaaa gcagaaatgg
    73441 attattccaa acatggcggt gccgttttgt ttggcttgaa agcaccagtc attaaaactc
    73501 atggagcaac aggccccgat gctgttcgct atacgatccg ccaaatccac acaatgcttg
    73561 agacagatgt agttggacaa ttagtcgaac aatttgaaaa aagcgaagaa tagtatttgg
    73621 atatctgaag cgatccgttc cattgaaaaa aaggtggaca ttcctgaaaa atgccagtaa
    73681 aattagccat ggtgtaggta attgacaagt gtatgattct tggaggtgaa aaagttgact
    73741 cgcgaagaag tatttaacaa agtagcgaaa attatttcaa atcattttga gatagatacc
    73801 gataaagtga cggatgaatt gaacatcaaa gatgatttaa aagctgattc aattagtatt
    73861 atggaattcg ttttagagct tgaagatgaa ttcggtacgg aaatttcaga tgaagacgct
    73921 gaacaaattg aaacagtcgg tggcgcagta gattatatca gcagccatct taaataattt
//
LOCUS       DARBDV010000004        66291 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000004 DARBDV010000000
VERSION     DARBDV010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 66291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.004"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..327)
                     /locus_tag="SCM56_000266"
     CDS             complement(<1..327)
                     /locus_tag="SCM56_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109098.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQ"
     gene            complement(344..1549)
                     /locus_tag="SCM56_000267"
     CDS             complement(344..1549)
                     /locus_tag="SCM56_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEG1109099.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            2243..2629
                     /locus_tag="SCM56_000268"
     CDS             2243..2629
                     /locus_tag="SCM56_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEG1109100.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            2723..2914
                     /locus_tag="SCM56_000269"
     CDS             2723..2914
                     /locus_tag="SCM56_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1109101.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            3428..3760
                     /locus_tag="SCM56_000270"
     CDS             3428..3760
                     /locus_tag="SCM56_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1109102.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            4056..4211
                     /locus_tag="SCM56_000271"
     CDS             4056..4211
                     /locus_tag="SCM56_000271"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEG1109103.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            4487..5827
                     /locus_tag="SCM56_000272"
                     /pseudo
     CDS             4487..5827
                     /locus_tag="SCM56_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(6068..7684)
                     /locus_tag="SCM56_000273"
     CDS             complement(6068..7684)
                     /locus_tag="SCM56_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEG1109104.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            7802..8020
                     /locus_tag="SCM56_000274"
     CDS             7802..8020
                     /locus_tag="SCM56_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1109105.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            8215..8676
                     /locus_tag="SCM56_000275"
     CDS             8215..8676
                     /locus_tag="SCM56_000275"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG1109106.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(8869..9108)
                     /locus_tag="SCM56_000276"
     CDS             complement(8869..9108)
                     /locus_tag="SCM56_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1109107.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(9132..10655)
                     /locus_tag="SCM56_000277"
     CDS             complement(9132..10655)
                     /locus_tag="SCM56_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEG1109108.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(10673..10795)
                     /locus_tag="SCM56_000278"
     CDS             complement(10673..10795)
                     /locus_tag="SCM56_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEG1109109.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(10825..11808)
                     /locus_tag="SCM56_000279"
     CDS             complement(10825..11808)
                     /locus_tag="SCM56_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEG1109110.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(11832..11981)
                     /gene="rpmG"
                     /locus_tag="SCM56_000280"
     CDS             complement(11832..11981)
                     /gene="rpmG"
                     /locus_tag="SCM56_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEG1109111.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(12002..12379)
                     /locus_tag="SCM56_000281"
     CDS             complement(12002..12379)
                     /locus_tag="SCM56_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109112.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(12411..12590)
                     /gene="rpmF"
                     /locus_tag="SCM56_000282"
     CDS             complement(12411..12590)
                     /gene="rpmF"
                     /locus_tag="SCM56_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEG1109113.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(12605..12874)
                     /gene="rpsN"
                     /locus_tag="SCM56_000283"
     CDS             complement(12605..12874)
                     /gene="rpsN"
                     /locus_tag="SCM56_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEG1109114.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(13397..15163)
                     /locus_tag="SCM56_000284"
     CDS             complement(13397..15163)
                     /locus_tag="SCM56_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1109115.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(15123..16876)
                     /locus_tag="SCM56_000285"
                     /pseudo
     CDS             complement(15123..16876)
                     /locus_tag="SCM56_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(16986..17555)
                     /locus_tag="SCM56_000286"
     CDS             complement(16986..17555)
                     /locus_tag="SCM56_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEG1109116.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(17573..18988)
                     /locus_tag="SCM56_000287"
     CDS             complement(17573..18988)
                     /locus_tag="SCM56_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEG1109117.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(18999..19667)
                     /locus_tag="SCM56_000288"
     CDS             complement(18999..19667)
                     /locus_tag="SCM56_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEG1109118.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(19797..20381)
                     /locus_tag="SCM56_000289"
     CDS             complement(19797..20381)
                     /locus_tag="SCM56_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1109119.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(20712..20945)
                     /locus_tag="SCM56_000290"
     CDS             complement(20712..20945)
                     /locus_tag="SCM56_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HEG1109120.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(20960..21910)
                     /locus_tag="SCM56_000291"
     CDS             complement(20960..21910)
                     /locus_tag="SCM56_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEG1109121.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(21907..22749)
                     /locus_tag="SCM56_000292"
     CDS             complement(21907..22749)
                     /locus_tag="SCM56_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEG1109122.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(22751..23470)
                     /locus_tag="SCM56_000293"
     CDS             complement(22751..23470)
                     /locus_tag="SCM56_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEG1109123.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            24162..24320
                     /locus_tag="SCM56_000294"
     CDS             24162..24320
                     /locus_tag="SCM56_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1109124.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            24392..25618
                     /locus_tag="SCM56_000295"
     CDS             24392..25618
                     /locus_tag="SCM56_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1109125.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(25706..26098)
                     /gene="rpsI"
                     /locus_tag="SCM56_000296"
     CDS             complement(25706..26098)
                     /gene="rpsI"
                     /locus_tag="SCM56_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEG1109126.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(26114..26557)
                     /gene="rplM"
                     /locus_tag="SCM56_000297"
     CDS             complement(26114..26557)
                     /gene="rplM"
                     /locus_tag="SCM56_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEG1109127.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(27076..27543)
                     /locus_tag="SCM56_000298"
     CDS             complement(27076..27543)
                     /locus_tag="SCM56_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEG1109128.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(27663..29201)
                     /locus_tag="SCM56_000299"
     CDS             complement(27663..29201)
                     /locus_tag="SCM56_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEG1109129.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            29281..29967
                     /locus_tag="SCM56_000300"
     CDS             29281..29967
                     /locus_tag="SCM56_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEG1109130.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(30168..30695)
                     /gene="msrA"
                     /locus_tag="SCM56_000301"
     CDS             complement(30168..30695)
                     /gene="msrA"
                     /locus_tag="SCM56_000301"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEG1109131.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(30779..31456)
                     /gene="pgmB"
                     /locus_tag="SCM56_000302"
     CDS             complement(30779..31456)
                     /gene="pgmB"
                     /locus_tag="SCM56_000302"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEG1109132.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(31453..32583)
                     /locus_tag="SCM56_000303"
     CDS             complement(31453..32583)
                     /locus_tag="SCM56_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEG1109133.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(32583..34766)
                     /locus_tag="SCM56_000304"
     CDS             complement(32583..34766)
                     /locus_tag="SCM56_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEG1109134.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(34763..35788)
                     /locus_tag="SCM56_000305"
     CDS             complement(34763..35788)
                     /locus_tag="SCM56_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG1109135.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(35833..36660)
                     /locus_tag="SCM56_000306"
     CDS             complement(35833..36660)
                     /locus_tag="SCM56_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEG1109136.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(36657..37724)
                     /locus_tag="SCM56_000307"
     CDS             complement(36657..37724)
                     /locus_tag="SCM56_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HEG1109137.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(37750..38586)
                     /locus_tag="SCM56_000308"
     CDS             complement(37750..38586)
                     /locus_tag="SCM56_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1109138.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(38583..39452)
                     /locus_tag="SCM56_000309"
     CDS             complement(38583..39452)
                     /locus_tag="SCM56_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1109139.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(39522..40805)
                     /locus_tag="SCM56_000310"
     CDS             complement(39522..40805)
                     /locus_tag="SCM56_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEG1109140.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(40819..42504)
                     /locus_tag="SCM56_000311"
     CDS             complement(40819..42504)
                     /locus_tag="SCM56_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HEG1109141.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            42779..43804
                     /locus_tag="SCM56_000312"
     CDS             42779..43804
                     /locus_tag="SCM56_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1109142.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(44399..48073)
                     /gene="rpoC"
                     /locus_tag="SCM56_000313"
     CDS             complement(44399..48073)
                     /gene="rpoC"
                     /locus_tag="SCM56_000313"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEG1109143.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(48131..51748)
                     /gene="rpoB"
                     /locus_tag="SCM56_000314"
     CDS             complement(48131..51748)
                     /gene="rpoB"
                     /locus_tag="SCM56_000314"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEG1109144.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            52476..53444
                     /locus_tag="SCM56_000315"
     CDS             52476..53444
                     /locus_tag="SCM56_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1109145.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            53462..54367
                     /locus_tag="SCM56_000316"
     CDS             53462..54367
                     /locus_tag="SCM56_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1109146.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            54364..55170
                     /locus_tag="SCM56_000317"
     CDS             54364..55170
                     /locus_tag="SCM56_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1109147.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            55306..56295
                     /locus_tag="SCM56_000318"
     CDS             55306..56295
                     /locus_tag="SCM56_000318"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEG1109148.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(56612..57136)
                     /locus_tag="SCM56_000319"
     CDS             complement(56612..57136)
                     /locus_tag="SCM56_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HEG1109149.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            57423..59120
                     /gene="scm"
                     /locus_tag="SCM56_000320"
     CDS             57423..59120
                     /gene="scm"
                     /locus_tag="SCM56_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HEG1109150.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSS
                     TTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDN
                     RTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(59275..60261)
                     /locus_tag="SCM56_000321"
     CDS             complement(59275..60261)
                     /locus_tag="SCM56_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEG1109151.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(60284..60850)
                     /locus_tag="SCM56_000322"
     CDS             complement(60284..60850)
                     /locus_tag="SCM56_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HEG1109152.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(60864..61280)
                     /locus_tag="SCM56_000323"
     CDS             complement(60864..61280)
                     /locus_tag="SCM56_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEG1109153.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(61737..63671)
                     /locus_tag="SCM56_000324"
     CDS             complement(61737..63671)
                     /locus_tag="SCM56_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEG1109154.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            63952..64908
                     /locus_tag="SCM56_000325"
     CDS             63952..64908
                     /locus_tag="SCM56_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HEG1109155.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            64998..66101
                     /locus_tag="SCM56_000326"
     CDS             64998..66101
                     /locus_tag="SCM56_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEG1109156.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            66114..>66291
                     /locus_tag="SCM56_000327"
     CDS             66114..>66291
                     /locus_tag="SCM56_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEG1109157.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAIN"
BASE COUNT        20253 a        13375 c        11341 g        21322 t
ORIGIN      
        1 ctgatccaat ttttgataat ccataaattc atcatgtatc aaaaagaata aataacttga
       61 tattttagca tctagcttaa tcagattaaa taatgtttgt ttaaaattgc taccattaat
      121 atgactgata gattctctag cttcttcaca taaacttttt actttattaa ttactacttt
      181 gtcatattca tttaacacac tattattacc attacttaga cgctcagatt ctacgaaaca
      241 actcaaatca gaaatttccc aaaaaaattt tttgttaaaa tccaatttcc tcaactcctc
      301 caaactacgt aaatgatata gtctcataat aaaataaaac ctctcagcta atgttgttga
      361 aattgtccac taaacctaat tgatattgct taggagatac acctgcgtac tttttgaata
      421 ctctcgaaaa gtgtgaagaa tctgaaaatc ctaaacctaa agaaacttca ctgatagtat
      481 ggtttgattt caacattatt ttagccgtag aaatttttcg cttagagata taattataaa
      541 tcgtaatatg catttcattc ttaaagcttt ctcttaattt agtttcactt acagaaaaat
      601 aattagctat ttcctttaca gtaattcttt tatagatatt taagccgatg aattgaacga
      661 cagaagaaat taagggagaa agatgctcta tcttagtttt tccgatttcc ttagtataat
      721 aaacaatccc agaatccctc acttttaaga cctcaggtaa cgatacagct aattcgtttt
      781 cttgtatcaa agcatctcta ctttgatacg cttctattat atccattcct aatgttatag
      841 acgtttgtgc taacttttca aaaacaatga tagaataatt tttttcagac cgaatcgtat
      901 ctccactaac acttggaatt attccattac tcagattaaa aaccatgttt tctaaatttc
      961 gaatatctcc atttttaaca tattccagta ttttattttc ataaagaaac agatatattt
     1021 ctggatcgta attttgtgat aacagattat ctattttgat ttgctcgata gttttactta
     1081 gattttctgt atattcaatt aacggttcat ggaatagatc ttctattttt ccagtaaaaa
     1141 aataatgaac taaaatcaga atatctcgaa tgtctcctaa agaatacaaa gtcaaatttt
     1201 gcaatgaatt atagagtcgt tctctatcag ccattgcaac attcaaattg ttcattttta
     1261 gtttaaaaaa aactttatca atattatttg ctctaaatgg accaaataag taatatctgg
     1321 ttttaaacat gtatagaaga aacatctcat taaaatcacc agtaaagaaa tggaaatctt
     1381 tagttttttt aatattatca gttagtaact ttttataatc ataaaaaaaa taagtagttc
     1441 tatctgagcg atactctttt ataatactga attcggaatt aaaaaccacg ataggtaact
     1501 ttaaacaaac atgaagagac tttaataaga cttctttatc tgaaaacact tttgtttctc
     1561 tctttccttt ctacatcatt aaatagtatt ttatacgtta tctaaaatac tattcctctg
     1621 tcaaaataag ctaacttact tcattataaa atatccactt atcgctttca tgtatattcc
     1681 accgtaaaaa gtacatttta tcaccgagaa ctcatcattt ttcatcatga tgaatactat
     1741 ttacatatat ctagttcaaa gacaataatt attatgatcc gttattatct aagcactata
     1801 cagataatag gaatttttcg tttctaaata ttccaatttg ggaatccaat aaaaattttc
     1861 gttcaaatac gtgtctattc gaactctttt ctagttgtta attatgaagg gggaagaaat
     1921 aaaaaataca atctatacac gaataagaat attatccaaa aggaggtttt agaaaagatt
     1981 tattatacgt aaaaagctca agacattttc ttagccataa ggacatgttt gatatttaag
     2041 aagatatatg aattttcttt tctaagtata aagataacta tccattatca actaaaaaaa
     2101 catttttgaa aataaacatc agcagtccta tcaatgcatg tgagcttata gaagttattc
     2161 gcagcgcagc ataattgcat tagcttcttt tcgtgttttt tgcctctttt ccctttaaat
     2221 cttaatggag gaggtgtgca atatgtcaga taacgacgaa cgcgtaaatg cccagttgat
     2281 tcgattcttt gaaaaggtca ttttgaacac cgctaatact tattataaaa aacaagaaga
     2341 aattcaaaaa catgaacaac ttgatcaact cccaccatat ttgtttacta aagatactat
     2401 aaaatttaaa caaccgatta atgttttaaa tgtgactctc cttgttgaag atataaaatt
     2461 agcaaaaatc cttcctttgt tgaaagaacc tgaacaaatt tttcttatcg aaaaatttat
     2521 ctttgataaa actgataaag aaattggaaa ggttctgggg attacaagac agggtgcgac
     2581 aaatttaaaa catcgacttt acaaaaaaat tcaaaaaatg ctggtttaat atctgcataa
     2641 taactaattt tgtatgcttc tttgctcgtt ctcctccttc ccaaagaaat agattataag
     2701 tccatgggaa gaaggtagat ttatgaattc tttaaaagaa gatttcattt tagcaaaggc
     2761 tggaaatgaa gaagctgttg aggcaattct caaaagattt agctctttga ttcataagca
     2821 atcctggaga actggaaaat atgatcaaga ttgttatcaa gaatgtatgc ttgcaatata
     2881 tctagctatc tcgaaattcg aaattaaaga ataactcaac taagggagtc tgtgacataa
     2941 gtaaaaagat agtttcaaat ttcgaacaat aaatttgaaa ctatcttctt tataattata
     3001 aagtatcgat tttacagcgt tctataatga ttcatagaat ctaaaacgaa tgattttaat
     3061 tatcaacgtt ttttaattcg tttttagctt aaaaataact tttgtcacag tctcactcct
     3121 aaaattactt tatttacgta tgaagtgggc aggaggtcga atccagaact gggctatggt
     3181 acttcaacga ggacttctcc tctagaattg aaaagcatgc gatttacctt ccgtaacgac
     3241 ggtagattta ttcaactatg tgtccaatca aaatttgtct gatttcaaaa atagtcaaga
     3301 gaattttggt tgttaaaaac tcttatctat caacttccac agtttagttg acaaaccctg
     3361 atactaagtt gggctatgat acacttttct tgactatcag ttacgttacc ggaaggaatg
     3421 aactctattg agaaaaacat atgatcgtaa atttaaacta aagatttcac aggatatcct
     3481 tgagaaaaaa gtaactacca agaaaattgc tgaagaatac aatatctctc gtccaacaat
     3541 ttcccgatgg gtttcagaat atcgtcgata cgggaaaaat gcttttgctg gacaaggaaa
     3601 aagattacct gataaagcag atttctacat tcttgaacag gaaaataaac gactaaaaga
     3661 ggaagacgag tgttctacga acacaaagat cgatacggat ctccagggat cactcaaaaa
     3721 ctttacgatg aaggaatcga aatcaataaa cgtgttgtag ctgttcttat gcgtgaaatg
     3781 aatttatgca caagaggatt tcatcgacgc aaatcttctc atggtagaga aaaaacaatc
     3841 aaagaaatgg taaaaaaatg ttgaatcgat aatttgaaca gaaccaaatt gatgctgttt
     3901 gggtgatgga tattacttac attccatgta atgacggtcg cctctatttg tccacctata
     3961 ttgacttagc aactcgtatt ctacgttact atatggtaga ttcacacatg aaaaaagaga
     4021 ttgtcgtccg tccattacaa atttattaag gagaattgcc tacggtaatt cattccgata
     4081 gaggtagcca gcatcggtct tttatttatc aagagctgtt gaacgaccac cacatcacac
     4141 attcagtgag ccaaactggg actcctgttg acaatgcagt aattgaagcg tttcatcgat
     4201 caatcaaatg agaattaatc gactccaaca aatataaaag taaaatagag atgaaggtgt
     4261 taatccaaga ttatttagaa gactactacc caaacaaaag aatccataca aaatttatga
     4321 tgactccttg acaatttgaa gcgaagaaaa taagggatct ataatatcta gtgttggtga
     4381 cgccttctgt tgccaatact ttttcttttt tctaataaac gcaaacagac accttaaaat
     4441 cctttagaat aaagtcgacg aaaaccattc aaaggagtgt ttcaagatgt cctattcaga
     4501 gtttaccaaa gagttactag atattctaga cctaaatctt atctttcatg aagatgcctt
     4561 tcgcaaagag cgaatcaatg acgaaacatg ttttgttttt gatggcacat tgacctatca
     4621 accggaggaa tgctttcatc gccactatca aaatgaacaa acgatcatta aatagggctg
     4681 gaaaaaagta tccatcttat tgaatgatgt cagtaactac aagaccattc tgcggattaa
     4741 caaacagcgc ttcaaatgta aacactgtgg aaagactttt ttagcagaag attcagttag
     4801 tgatcgtcgc tgctccattg ctcgcagagt caaacaagcc attcttgagc tattaagcga
     4861 acctctttct atgtctttga tcgcacgtat gaagcacatt tctccgacca ctgtcattcg
     4921 aatacttcgc agccttcggc ctaaaaccat ttctttgagc cagctgttac cagaagtggt
     4981 gtgtttcgat gaattcaaat cagtgaaaaa tgtctcggga gccatgagct ttgtcatgat
     5041 ggacggcaag acccatcaac tgattgatat tgtggaaaat cggcaactca atcctttacg
     5101 cgactatttc ttgcgctacc ctataaaagt ccgtgaacaa gtgcgtctag tggtttcaga
     5161 tttctataca ccctatcgca cattggtgaa agagtgtttt ccgaatgccc gaatcgtcgc
     5221 cgatcgcttt catattagcc aacacattgg tcgggctttt accaaccacc gaattcaagt
     5281 aatgaagacc tttaaaaaag gggatcgtcg gtcgaagcac ttaaagaaat actggaaact
     5341 acttcaaaag aatgcgtggg aattgaaagg tcagcatcgc tactggcgtc cgtcatttcg
     5401 agatcatctg acagaagcag agatagtcga tcggctatta tcttatgatg attccttaaa
     5461 gcgaggctac gaagtctacc aggattttct ttccgttatc agaagacaag atgttcctga
     5521 attcgtcgcg ctcctaaaag aagactacaa ggagttgcca gagcactacc aaccagtgtt
     5581 cacgaccttc aagaaatacc aaacagaaat caaacgagcg ttgcgcgttc cctattccaa
     5641 cggccctata gaatgtttga ataaccatat caaagtacta aaacgaattg cctatggcta
     5701 tagaaacttt caaaactacc gagaacgaat ctttttatat cgaggaaaat attttaagaa
     5761 aactaagaat actacccaac tgactaaagc cagaacaaca acacggctag acaagatagc
     5821 tgtctagccg tgtgagtatc tcgattattc tattgaattt cttcaccaac actcattgac
     5881 gaagagccaa aataagtaat ctaaccgact gttaactaaa ccattgcagg tcccgttaaa
     5941 gatacttgtg cctgttgtca ccgcttcttt ttcttgtcca caagcgctta acacacttaa
     6001 taaagcccct gctattatta ataataatat ctttttctta ttaaaaacct cctcatctat
     6061 ttccagatta gtaaatgtag taatctgtgc tttctttttt ctcaatacta ataactaact
     6121 tatggggtaa acaaatactc gtttgaccgg gttttgagat ccatccagtt ctaaccgcga
     6181 tttgatcagg actattatct tctttaactc gaattcttcc atctttaatc tcaacaatat
     6241 tatattgtcc cttcgcagga tgataattca cgagtttatg attgatctta tccaaatcga
     6301 agcgatccat ttccttgcca tcaattcgaa cgatggcata tttgatagca tcttcagttg
     6361 tttcattctc cttttctata gagaatacaa ataagggaat aaaagaggta ataatcaaaa
     6421 tgccaataat tatgacatcg cctatcttga caccagcttt tcttaaaaat ttcattaacg
     6481 atatctggct aaacatatta gactttttcc atggaaatgg tgaaattttt cggaaattat
     6541 ctgcattaag agaatcagtt aaacaaccct caagttgttc cttaatacat tgttgatcca
     6601 tcttcatacc aataaaaact agtttattaa tgcgttcacc aaacaacgga tgccaggctt
     6661 ccttcaattc gggatttttt tgtataatca tttgacactc aattttaggt aaactagcta
     6721 cccaataatt taatgattca atacttttat agctacccga tgtttcaaag atcgtcgaaa
     6781 aatcactatg ttgggcaaac caaagaaacc cttttgctct tgtaatattg ccaggaaaat
     6841 ttttatccgt aagatgggca aatttttgtg cgtcaaggag taaccgattt cgatagataa
     6901 aacttgagat accatattct tctgtttcag gaatatgatt ttgatagcca agctctaact
     6961 ctttgaacca gcccactgaa taggaagtct catctatatt aaaaagattt gtatttagaa
     7021 ttttcgataa gtctacttca cctttaattg tcctaattat tttggcattg gactgtaact
     7081 tagcaattag accgttaatt ttgtaaatcg tcttggttga cactaagtca ctattattta
     7141 aaagtaatat gttacaacac tcgaattgat caattaacaa gccacgaata tcctgttctt
     7201 cttcaactac attaccttca gcttttcttt cctgaatctt ttcacccgtt tcgaaattat
     7261 cccaaaaacg ttttacatcc actactataa taatggcacc aatctctaat cgttgggtca
     7321 aatcaatgtc taaagggttt ttagctaata taattgcttg aataattgga attggtttgc
     7381 tgattcctga agcttcaata acaatttgat tcaagtcaga aaattgcact aattgatcta
     7441 gttcaacaat caaatcttcg cgtaatgtac aacaaatgca tccattcggc atcgaaacaa
     7501 gtttttctcc agttctttta aaacctttct tgtcgactaa taccccatca acatttactt
     7561 cccctatgtc atttacaatg acagcaattt cctttccgtg atcaccgtac agaatatgat
     7621 ttagaagtat cgtttttcct gaaccaagat agccagttaa gattgtaata ggtatttttt
     7681 tcatagggaa cctctttcta taccttgaat ttacacttta agtgcaacac ataaaaatga
     7741 gtcataatcg atatccaact agtataagtt tgtcgagaac actcctagaa ggaaaataat
     7801 tatgagccag tatcgtcatc ttactatact cgaacgagaa agaatctttc tgcttcacga
     7861 agaaggattt tctattagta taattggcaa cgacattcag catagcccgt ttactatttt
     7921 acatgaactg agaagaaaca agaaaaaaac tattcacctt ccaaagcgga aaaaaattat
     7981 gcttatcgaa gaaaaaaatg cggacgttat cacattctaa atgatccgtg agtttggtca
     8041 attgttcgtc atttatttgt cgattttcag tggtcgccag aggaaatctc gaatcgtttg
     8101 aagcatgaac atatggactt ttcaattagt tttatcacga tctaacgtgc aatttgcaat
     8161 gatcttttcg atacagaacc cctctcacac ggcaataaag gcctgatccg ttcattgcac
     8221 catcgaggaa aaacaagaca tacaaaaaat catgtcgagt ctcgtggaaa gatttgcctt
     8281 actcattcga tccatggccg tccaaaatcc gcagatgata gaagtgattt tggtcattgg
     8341 aaagccgata ttgtagctgg caaaactggt ggtacttgtt tagtgacttt gacagacaga
     8401 aaatcatgct tccttttagc aggaaagaag gtccccagaa agaaagccgc ttatgtgagc
     8461 gagaaaatga ttgaactttt aagcagtatt ccaaatgagg cggtcaaaac gatcactcca
     8521 gatagaggaa aagaattctc caatcatttc gcagttacag aagcattgaa taatatgccc
     8581 ttttattttc cagatcctca tgcccttggc aacaatgaac aaattaaaat acgaatagac
     8641 tcattcgaga atacttgcca aaatcgaaag agatagctac ggtagataac tcaacgataa
     8701 atcagatgat tgaaaaactg aataacagac ctagaaagtg tttagattgg agaacacctt
     8761 atgaagtctt ctttgataaa gtgttgcgct tgatttgaca attcaagaca taataaaaag
     8821 cagatcaaat ccaaataagg gaaaaatgat ctactctggt attatcaatt aattggccaa
     8881 gctattatgc cgttttgcta aacgtctact tcttccagta ttgaccttga taaaacaaca
     8941 atcaggatcc cgttttaata ctctatgaac tgtgtcaata ctaacatgta aagttgcagc
     9001 aatatcagct aatgtactcc catttaatct catttgcttt gcaaatcgat aacgccgata
     9061 agcatcaata ttcttggaat ccatagacaa taatactttt cttaccatgt atttcctcca
     9121 aaaattgaat tttaatgagc catcatttct tgagcaattt catgtttaga caatgaagct
     9181 ggatagaaag taggccaatt atgcaattca ttatagagtt tttcttgact ttctccacca
     9241 aagaaaatat ggaaatgctc tgccttactt ggtgcaattc catgatcact aaattgaata
     9301 tatttaaaac gaccagcacc tgcatcatca gtttcaaaat taaaacgaac accacggttt
     9361 cctttttcat aattcaaaat tttgtatcca acatatttgt atgtgtattg atgatgttca
     9421 ccgttgacta caaaatcaat ggtgttgtct ttaatgttga tagtatcaat atctgtctta
     9481 tagccagtag tataataatc tttgtactcg gcagctgtca tgtctttatt gatttttgct
     9541 ttgtaatcga aaacttcatc caaaattcca tcttttaaca acggataaac agattgccat
     9601 tcgcctgtat aatcactcaa ttcgcgatcc ttcacatcgg catctttaaa gtacccatta
     9661 gcaactgtct tttcatcctt cgcttctttt tctggaacta cctcttcacc agcaaccata
     9721 gttgtttttt ctaacgcttt tagattgtct tccattactg aaacataatc ttcaccatta
     9781 tccatttgtt cttttgtaag actttcaaga ggattcagta cttccaattt tatcccagct
     9841 tcgttggcca aagtttttgc aattttatca ttagcattct tttcaaagta aatataattg
     9901 atcttatttt ttttaacata ctcttttaat tcagctaatc gaccagaaga tggctcttct
     9961 tcaggactta gtccagcaat aggaacttgt ttcaatccat aatccaaagc taaatatcca
    10021 aaggctgcgt gttgcgtcac aaaacttttt tgtttcgctt ctttaagact agtggtatag
    10081 tcagcatcca accgtttcag tttagtcaaa tacttttcag catttgtctc aaatactttt
    10141 gcttttttcg gatacaattt aactaactga tccttgatat ttgaaacttc cttaattgcc
    10201 attttgggtg aaacccacgt atgaggatct agttcatgat ggtgatcttc ttctccgtga
    10261 tcatgatctt cttcttcact acctggcaat aaaatcatgt cttttgttcc ttctactaca
    10321 ttaggcccag ctttcttcca actttcttta gcttttggta cccatgattc catgttttcg
    10381 ttatgatata caaaaacatc agcatcatga gcttttgcca tatccttagc tgagggctca
    10441 tagtcatgag gttctgatcc cgctggaacc attaaatcca cgttcgcttc atcgccgacg
    10501 atgtttttgg tgaaatcata cattggatag aatgttgtta caatttctaa ttttccatct
    10561 ttatcattcg atacttttgc atcattagtg ctggagcaag ctcccaataa tcctagagct
    10621 gctatcccaa taagtcctac actgattttc ttcattcttt ttccttcctt aattatgcta
    10681 atttcttcat tttatttcgt ttatgttctt tgataatttt taatgccctt tttctagttt
    10741 taatattcgg gcttttaagt cgtcgatatg cactagatat atccgttttc tccattagaa
    10801 attaccccca atcactactt atcattattt gctttccgcc agtaatttat caaaactctg
    10861 tttgatcctt tcttcatcca acatttttcc gattagaata atagtggttt ggtctttttg
    10921 tcgagtggtg tattgaaaat taacctgttc gtttacctct tgcatggcaa ctataaaatc
    10981 ctgctcttgt aaagcaatca atcctttaaa acgatacagg ctcccttggt tggttaaaat
    11041 cagccagtca atccattgga gtaaaaaatc tttttttatg tcttgatctg aagttaagag
    11101 aattgattta aactcatgta cttgatggtg ttgatgatag tatttgccat tatagtgatt
    11161 ttctgatgaa tcactttcaa aaactaaggg atgattaaat tttttcaaat tataaaaatc
    11221 ctttttttga atcgtttcac tatgtgtgaa cattcgaata tcggataaag ggttaatact
    11281 tttgatttct ctctgtaatt tttttagtgc agataaattc aagttttgtt ttctagacac
    11341 aaaaatgcga tctgccatga caagttgctt aagggcttgt ggttctcttt caataagttc
    11401 aaaactgtca ctatcaataa cagtcaataa actatcgata tatactcttc ctcttagttg
    11461 aggcgctgtt aaaagtgttt gaatgattgg ttggggatct gctatacctg tagtttcaat
    11521 gatgatttgt gaaattggtt gttgatgttc ttctattgtt tccagtaaag aaatcaatat
    11581 tgctaataaa tcactgcgaa gactgcaaca catacatcca ttatttagtt gtaacacccg
    11641 ttcctcgcta tgaactaata atttgtgatc aatcccaact tcaccaaact cattttcaat
    11701 tataataatt tcttccctgg ggtagggtga gacttgcaag gcttgattga ttagcgttgt
    11761 ttttccagcc cctaaaaacc cagaaacgat tgtaactggt atgcccattt tgtcaccttt
    11821 ttaccactta gctatttcac ttctttaaaa attgcccgtc tgcgcaattt tggcgaatac
    11881 ttttttaatt ctaaccgttc agggttattc cgtttatttt tgctggtgag atacagacgt
    11941 tctccagttt cgacacattc caaaataata ttttgacgca taaatcttcc tccttcaata
    12001 cttatcgatc ttcaagatat gcttgaattt gttgtgcatc ttcaaaagtt aactcgccag
    12061 attccaattg tgaaactaaa ttttctttca tatcaacagg tatctcccaa taaccatcga
    12121 tacattgaaa taactcctcg tcatactctt caaaaattgg atagattctt aaaaaatcat
    12181 caaatgataa gccttggtcc tcatagtaat ctttgactaa tccattttca attgcctgtt
    12241 gggtcggtaa acccgtttca gtaataattc ctctctctac catcgaacga taaaaagtgc
    12301 atttatctag cgattgctgg gtcgtttcaa gataatactt taatccttct tcaactaaag
    12361 gtgggtaaat aatttccatt ttgattccct cctaaaagtt atatattaaa ctattttgaa
    12421 gctcctttaa ttactttctt tccattatag taccctttta aagatacatg atggctacgt
    12481 ctataatcac cattcgcttc gtcaaatgaa atttctggtt ttactacagt ttgatgagtt
    12541 cttcttaacc gtttcttcgc cttagatgtt tttcttgctg gtactgccat gatatttcct
    12601 ccttttacca acttgctttt tttactccag gaatcaaccc ttgatgggca agttctctaa
    12661 acttaatacg ggacataccg aactttcgca tataccctct tggacgacca tccgtctgat
    12721 cacgaagcct cagacgattc ggatttgaat ctcttggcaa tttagcgaga gcggtgcgat
    12781 ctcctgcggc ttttaattcc tgacgtttat ctgcatactt ttcaatcatc gccatttgtt
    12841 tctttgcttt agcaattttc gacttttttg ccatatacct ttatactcct tttattttga
    12901 tttagacacc tcaaatcgta atcgttacgt tttgacactg atactttacc aacaagagtt
    12961 gctcttgtca acaataaata acatgaaata aaactcatat gaatagataa ctatttgaaa
    13021 acgaatttat cggtacatag agataatcaa atcatttaaa agaatatgca gtttaggctc
    13081 tgaaaaataa gaattcactt ttattagtcc aatcaaacac aaataccgtt ttaagtagct
    13141 tttcctctac ttaaaacggc aatatgaaag aaactataac tttttattca atcttttatg
    13201 tttaactggt ttatcgccaa ttttttaaaa ctttgattat tccttatatt ataagaatag
    13261 acattaagtg aactaattca atcatactga ttctttcgtg tattcaaaat tagatgctgt
    13321 ttaataaaat tattcttttt gaaaaagaat tataactata tcccaaaaat tgtaggttaa
    13381 tttaaagcct atttcatcat cgtgattgag cttctttttc atagacgaat ttctcagata
    13441 gtttagtttt ctttatgaga tttttgtata tttctgacct tcgatacaac tcattatgag
    13501 taccttgatc aactagtttt ccttgatcca tcactacaat tttatttacc ttttcaatag
    13561 acttcaagcg atgcgatata attagtactg tcttatcttg tattaaacga tttaaacttt
    13621 cctgaatacg tttttcattt tccgcatcta aactggcagt aatttcatct aaaattaaaa
    13681 ttggcgcatt tttcaaaaaa gcccgtgcta tggataggcg ttgtcgctct cctccagaaa
    13741 gtgaacttcc gttttctccg accaatgtat gataaccgtc aggtagtttt tcaataaagt
    13801 cttcgcagtt agctaaacga gctgcttttt tcacttcctc atctgacgct tgactatttc
    13861 caatacgtat attttccaaa atacttgtat taaacaaagt tacctcttga aaaacaatag
    13921 cgattttgct aaacaaagaa gctggagata cacgtttaat atcataccca tcaattaaaa
    13981 tacagccttc atcataatca tacagtctag acactaattt caagatacta gttttcccgg
    14041 aaccactagc acctactaac gcagtcactt ctccttgttt agccgtaaaa gagatatgat
    14101 ctaaaattgg ggtattgtta tcatatgaga aagagacatc tcgcaactca atgtcaaaag
    14161 atttcagagg actatctgat ccctcttgga tactggtttc cttcatagct ctaattcgct
    14221 gtattttagg agctaaataa aatatttcaa gcactgcttc tttcatagaa tcgaaggaat
    14281 cctttatttt tattgcggct agtaagtaac caacgacgta gagtatagtc acttcacccg
    14341 ttaacagtaa gtgaacacct actagaatga ctactgctaa actaacatat gagaaaatag
    14401 aggataaggc catgactgaa aaagtactta attctacctt taaatgaatc ctttcactct
    14461 cttccatttt tttgaaaagt ctgtcttgaa cttttttaga tagattaaag ctattaatct
    14521 cttgctgcaa ttctatagtt tcttgaaatt cttccgagtt ttctctcaaa gtatcgtaat
    14581 atttagtatt ggctttcgtt tgggatttct ttgataacaa gattaagaca aaactaaata
    14641 acgttggcaa aataacagct aatcccattt tgacattacc aaccaaaagc atcactgaaa
    14701 taaaagggaa aaacagagcc ataccgccgg ctttaggtat tgcatgactc atcgcatgct
    14761 caataccttc aacgtcagac atgatagttt gagataaatc tgataaatta tgttttgaaa
    14821 aataggataa tggtaaattt gacaaatcgt ctgcaatttg cactcttaaa tgcgcacttt
    14881 ctttataagt actattatat aagttttcat actccctatc taacaaccaa tacatgaaaa
    14941 gcaaagtaac aaacgatacg gcaaaataaa gccaacgggg ctttgtattt tctaaaacta
    15001 attcgtctat cagcatcatt ataatgaatg ctggtaccat attaatgcag tatacaaaaa
    15061 aactagaaat agaagcttta gttaaagctt ctgcgccctg atcacttaaa gcaaatttgt
    15121 ttttataaaa acttttcatt gtcaagcctc caatcatttg cattctcata attagtgacc
    15181 aattgtttat acacttgatg tttagccatt aattcattat gatttccacg ttctactatt
    15241 ttcccttctg acaagacaag aatttcatct acagatttaa tgctggataa tcgatgagct
    15301 atcataataa ctgttttttc tttcatcaga tgttgaaaag ccttttgtag ttcatactca
    15361 ttatctggat caatagatgc actggcttca tccataataa caatctttgc atcttttaaa
    15421 attgctctgg caatagctat acgttgtttt tctcctcctg acagataaac tcctttacta
    15481 ccaattaagg tatgctctct ttcgggaaat ttttctaaaa tcgattcgca accggctaat
    15541 cttagcgcgt caaatacttt acttgctggc gcttgaggat ttgctataga aacgttatcg
    15601 taaatactct gattgaatag tttcgtatct tgaaagacaa aagcaattgt gttaataatt
    15661 gtgtcttttg aatagtgatt taaaggctta ccaccaattt taatctgtcc cttatccaca
    15721 ttgtagaaac ctgaaattag tttagctatc gtactctttc cgcttcctga ttttcctact
    15781 aatgcataaa ttcttttttg ttctaaaact aagttgaaat tttctaaaac acactcttct
    15841 ttgtatgaaa agctgacatt ttcaaatgca atgtcacaat ttttaatctg tgtctcgttc
    15901 ccatatttta gttggtcttg cttcatttct aaataaaggg agtctagtgt ttcgactgca
    15961 tatcttgctt caaacaagtg catacttaca tacattattc tcatgaaaga aacaaaaata
    16021 attccactta aaaaaaacaa catcaacaag tcaacagcta atatcttggg agtggtaaga
    16081 gaagttataa aaaaggttat aggaactatc aaaatgcaaa ctatgccaaa aaatagccat
    16141 tgaaaaagaa caaaaggtgt tttcccactc aaagaatact tatatgcaaa cttagcataa
    16201 tcgttaattg ctttattcaa ggctttaaat gatgttactt tagcaccaaa tatctttata
    16261 acttgaattc cgcggatata ttctaccgtt tcagagctta acacatctaa cgattcttga
    16321 tatttttgaa taaaatttgt attctctgtc attttcttta acaagaatac accaattact
    16381 aacatggcga ttagaaccaa tccaatttta aagttaatga aaaagcctaa ggcaactgat
    16441 aaaacaggtg ttactatggc ttgagcacta tctggaatca agtgagcaat tgccatatgt
    16501 gttttagccg cgttgtcatc aattgtcttt cgaatatatc ccgaagaatt taaatcaaaa
    16561 aaacgaaaac ttgcttctgt taaaccgcta attccctttt ttctcaaatt agtctctaag
    16621 cgaaacccaa ggatatgaga aaataagcca gaaaaaatat aaaataaagc tctaaagtta
    16681 accaagcaac agtttgaacc gcataactct tagctaaata ttcatctcct acaataatta
    16741 gagactgaag aaatttgtaa atactgtaat atccatagac tgttaatacg acagaaaaat
    16801 cagaaaataa agtggctaac catccgtagt atttcatttt agggacataa ctaaataatt
    16861 ttttgtatac tttcattttt tcctcccccg cattcaattt cttctcttat tcgtttacaa
    16921 ctgttaatgc tttccgtaat agcgtaaatt agagatgtac aaacagataa aaggtaaact
    16981 acgttctatt ttttgtattt ttttaagact tttcttccaa tcaatacacc aatcatagat
    17041 aaacttgcta caactaacac tgaaatgatt ccccatatac ctaccgcata gtggatcatt
    17101 gcatccattg ttgcagcaga ttgtccttta tctatccaat cttgacgaaa acgatctgta
    17161 aagaaccata taggaatcca ctgtccggca gcactgccta attgcattag agcccaagac
    17221 gcaacttgag gaattgctcg atcatatcca aatacagata gaataatatc agctaagatc
    17281 gccatactaa tcgaactgat aaaccaaggt aagtacgccc cgcccattaa cgtaaaaaat
    17341 aacaataaaa caattgtgta aatagtaaaa attccttttc tagggtagtt atagctaata
    17401 taaacaaata tgattccaga taataatcca aaaatcccgg gggatatgct gtgaccaaac
    17461 gccccaccaa tactactaac cattattgaa aaaaagtaaa ttactaggga aataactgta
    17521 gctaaaccaa taaaaatata atcttttgtt ttcatatata ttctcctcaa ttctatttcg
    17581 ttatgtcatt aaaaatctgt tctatcaaag ttgaatcttc tactctaagt tttatatctt
    17641 tttttattcc accatttttt aaataaataa ttctattgca tgttttataa agaaattccg
    17701 gatcatgagt agctataact actgcaccct tttcttttaa gtctttaata aggtcagcaa
    17761 tcctttccat attaatataa tccaacccac ttgtaggttc atcaaataga aataaattag
    17821 tttttgacaa tgaagctata gaaaccaata atctttgttt ttctccaccg gataaatcaa
    17881 acggtaattg atctttcatt ttataaagtt ccattttttt taaaattttt tcttgctgat
    17941 ctttactaac tatttcattc cccagggcta actcgctcct cactgattct gtgaaaaatt
    18001 ggtaatccat ttcttgcatt actaaaaacg gcgatgacaa atttttttta ttttcgatta
    18061 tctttccttt ggtagggcga ataatcttca tgagtattct tagtaaggtt gttttcccca
    18121 caccgttttt ccctaccaaa ccaataacat ctccaacttt gacatcaaaa tttatgtctt
    18181 ttagaatatt ttcataagta atttcttcaa cttttaatac atctgcactt accttttcga
    18241 tatagggaac ttcaatcgaa ctattaaaaa tgtcaaattt ccttaatgcg ttattgtttt
    18301 cagtttttaa caactgaata gttgtagttt ttacagttct atttttaaca aatagaactt
    18361 gatcaattaa ttctttcaaa tagtaaaaac gatgttcagc tataacaata gtaaatcctt
    18421 tattttttaa atcacaaagt aagtcagcaa gtttcttagt cgctttgaag tccaaatttg
    18481 aagacggttc atccaatata attaatttag agtttagcga taaactacat ccaatagaaa
    18541 ttttctgccg ttcaccactt gatagcgaaa aaatgttttt atctaatata tttgaaagat
    18601 ctaataattc agctaattca ttgattcttt tatccatatg ctcttttgta aatccaaagt
    18661 tttccatcga aaatactaac tcactcgttg tatttgttgt aaagaattgt cctctaggat
    18721 cttgacttac gtagccaata ttttttgata tactttcttg tgttaaggat tgtagcgagt
    18781 tattttcaaa taaaatttcc ccaaccaatt ttccgtcata aagtcttgga ataagtccat
    18841 tcattaactt taatagtgtg gatttcccac tcccagaaga accagtaatc aatgtaagac
    18901 tgcctttttt tattgaaaaa ttaaggtcaa caagcgcttg ttccttaaca tccttatatg
    18961 aaaaagaaac attgttaaat tcaaacaatt ctttcacctc aaatcaaaat acttaagaaa
    19021 aaaatagtaa cggaaaacag gatgatatag tctaataagc tcatgtttgt tttatagtaa
    19081 gaacttcttt tcacaggaga ttctatcccc tttgttattg cattagcggt catttcttca
    19141 gcaatcatag ttgcacgtat aagaacggga actaacagaa attccaaata ttgcagaggc
    19201 tgaaaaaaag acaagcgaat gtttctcatt tttaaagatt ctttaatcat tcttatttca
    19261 gaacgataag tcggaaagaa tctgatcact actgtaatac ttaacattat ttttttcggt
    19321 aaatgtattt tttctaaaga ggataataat tcattgctag tatagacact aaccaacaac
    19381 catgagatca ttacgaccgg caacattcga aatacaatat ataaggatgt cgcaaaaaat
    19441 atagtaaaaa actctatctt gatagcatca ataattttca ttactaaaaa cagaactgaa
    19501 taaatcaaga tgaaataaat acatgtcttt attttacgaa acatcaatac taaaactgta
    19561 gcaataatga aacaccccac ttgaaatata tttccctttc ctaaaaaaat aatgaccgga
    19621 aaaaaaatat ttaaagtaat caaggttaac ggattgattt ttgacactct ttcacctcca
    19681 aaaaataaga tcgcactgtt atcttaagct gataaaaatg caccgttatt cagataacag
    19741 tgcgatctta tcaaaaagca taactgagaa caattctcaa tgcaagcttt ttttaattaa
    19801 ctcagtaatt caatccaccc aggaacagaa aattgataca atcttctggt taatagcata
    19861 gtcttttctt ttgaatagct atgcttcaat atatcataga atgtagcgta gaatgagtaa
    19921 atgtagaagt ggatttcttc ttcattaaag actaaatttt gattgatcat tccgatagtc
    19981 tttaacttat ctagacaaca gactgtcttt ttcgtcacaa atgatgtaat tttttcaaca
    20041 atatcttgat atttggttcc agctgattgg aataaaatca aatcgaatat acgtttattc
    20101 tcaaaaatat aattaataaa tggatctaat tcattgaagt tctctctcca acttttctct
    20161 atatctatat tttctaagtt taagaggtat ttggtaaact cttcattata catattaata
    20221 agcgcattaa tatgcggctg aataatttct tcaaataacg cttccttaga agtaaaatgt
    20281 ttataaaaag cacccgtagt tacgcctgcg gtcaggcaca gttgccttat cttagtttgc
    20341 tgaaatcctt ctttttcaaa atggcttagt ccactttcta ttaacttttg gtgagttata
    20401 atataatctc tggtttctat gggtacttcc tcctaataag atgaattaac tttattatct
    20461 tacacaggac ttgaagtttc aatcgttaac gtttattact gaactcattc cttagagcta
    20521 aactaatgag aaagagcaga ataactgata caaaaagcaa tcctacaaga attaatagac
    20581 tcatctaaat cattcacctt ttctaaaata atccgagaaa gatttaaaaa aattatatcc
    20641 agctattagg ataagtccta tcataatcac tccaatagcc attttcttat cctccctaaa
    20701 aataaagtct tttacttaaa accttttttg taatcgtcaa taaatttttt ccctttatca
    20761 gttaattgaa atcctatatc taaatagtcc tcatatctaa gtttagccag catctcactt
    20821 acagatgctg gactaacact tagaactttt gctaaatcat tattttttaa tttctttttt
    20881 ctttttgata attcctgaat tgctagtaaa taaattagta tagtagtcgt tttaatttgt
    20941 accataccta ccctccaaat tatttcgaaa ggccttcagc aattttatcc aggttccatt
    21001 tcatcatcgc atagtaacta tcaccatctt gtcctttttc tgcaattgaa tcagtaaaaa
    21061 tcgttgaata gataggaata ttggtatctt ttgatactgt tttcattggt ctatcatcca
    21121 cactgctttc tacgaataag gagggaactt ttgttgtgcg taatttttca actaagtgtt
    21181 ttatttgatc tggtgttcct tcttcttcag tgttgatttc ccaaatgtaa gcagaaggca
    21241 cattatacgc tttcgagaaa tatttaaagc atccttcact tgtaacaatc atttttttat
    21301 cattcggaat tgaagcaaat ttagatttag cttctttgtc tagagaatcc aatttttcaa
    21361 tatacttatc tagattttct ttatagaatt ttttattatc aggatctttt tccgctaact
    21421 gtttttcaat atttttagcg taaataatac cgttttctaa atttaaccaa gcatgaggat
    21481 cttccttccc tttctcactc tgaccctcta agtaaataac atctatgcca tcacttgctg
    21541 caaaatagtc tttgttttcc tctttgttcg catttttaac taattttgta aaccaagcat
    21601 ttcctccagt ttccaagtta acaccgttat aaaaaatcaa atctgctttt gaagtttttt
    21661 gaacatcttc aggcaaaggt tcatattcgt ggggatcttt cccaataggt acgatactgt
    21721 gtaaatcaat tttatctttt gctatatttt cggtaatatc tgctaaaatc gaattagtaa
    21781 ctactacttt taatttttcg ttcgaagcca ctttatcttt gtctttacta tttattgaac
    21841 atgccccgaa tataaaaaga gcaatcaaaa tccccagact tcccagtact gctttccatt
    21901 ttctcatcat aacgatctct ccttctttct taaaaatttt tgttttggtg agattagaaa
    21961 actaattaca aataagaatg ccgctgttaa aacaatactt gatcctgctg ctatgttaaa
    22021 actataacca ataaacaatc caagcaatga tgaacttgct ccgactgttg cagaaataaa
    22081 tatcatagtt ttcaagctat ttgcatataa atatgctgta gctgctggtg taattaacat
    22141 tgcaacaatt aatatagttc ccacactttg catagctgtt actgctacta aagttaatag
    22201 aaccatcaat agataatgat aaaaactgac attcatccct attgcttttg ccattaatgg
    22261 atcaaaagaa gtgattaata attctttaaa aaatagaact actataacca acactaaaac
    22321 cgaaacgcca attgtaatcc acttatcaat atcttgtaca gctaaaatat ttccaaataa
    22381 aatatgaaat aaatccgttg agctatttgc aactcctata aggataactc ctagtgctaa
    22441 aaaagaactg aaagtaatac caattgctgt atctcctttt attacactat tgctttttat
    22501 aaaagtaata atgatagaag ctaatagacc aaatacaatc gcaccaataa aaaaattaat
    22561 acctaaaata taagaaattg ctactcctgg caaaacagca tgagaaatag catcacccat
    22621 aagagacatt cctcttaaaa taataaaaca acctactgtt ccagaaacaa tccctataac
    22681 tacagatgta attagagcat tctgtaaaaa gtgaaagtca tacagaccat taataaaatt
    22741 ttgaagcata ttattccccc tctccaataa atatagtatc tccataggct ttttttaaat
    22801 tttcttcatt aaatacagat tctacacttc cgtgagcaat tagatttcga ttcaaaagaa
    22861 taatattatc aaaatacttc tttaccttgc ttaaatcatg atgcacaatt agcactgttt
    22921 ttccctgatg tttcaatttt tttaacgtat ccataattat tcgttcacta actaagtcta
    22981 tccctacaaa tggttcatct aaaaatatga aatccgcatt ttgagcaaga catcttgcta
    23041 aaagtacacg ttgaaattgt cctccagata attctcctat ttgatgatta gaatattttc
    23101 ccatatcgac cttttccaaa gcctttgaaa ctctttgcca ttctgcattt ttaattcttt
    23161 gaaaaacttt cattccggca tatgttccca tagatacaca ttcttttact tttattggaa
    23221 aagtaaaatc aatatcagct ttttgctcta catatgcaat cttcaccagt tttttattga
    23281 tatcttcatt attaatggag acctctccct gatgagggat aatatttaac attgctttaa
    23341 gcaatgtgga ttttccagca ccatttggac caataatacc tgttaaagca ccttcttgta
    23401 tttttaaaga aatctcgttc aatgctaaaa aatcgttata acaaacatta agctttttca
    23461 cttctaacat aactttctcc ttctaaatga taatgattat cacgatcata tggtatacaa
    23521 aaattatatt ttagtcaacc ctaaatttta tagtttagat ttttttctgt tgctgaagat
    23581 tagaatgaca aaactatttg aaactacgcg tatagtaagt agctttcaaa atatataaaa
    23641 aaggagcgtt ctcaaatgag aacatgctcc taaaactact ttattggcgt atggaaaaat
    23701 tatacttgaa gtggatagcg ggttgaataa gttgataagc atcaaaacat ttataaaaat
    23761 tattcaagac tatgtctgtt ccatatacaa atatgaaaat cagtaaactc catttaactt
    23821 ttttcgtagt gtgtcttttt atacatgttt atcgttatta atagtgaata ggaaaaaaac
    23881 tatttttgat cattgacaat taaatacaat ttttgaataa cgatccctgc tattcgagct
    23941 agaaactcaa gtacgccatg acctgattag cgacaaatac ttagagattt ctttttgttc
    24001 tgggaaaaca aaatgccatg acaatagcag caaataatga tacttccgta aggctcgccg
    24061 taaactgggg agaggtgaaa ttcctatgtg cttacccaaa gcaattcaaa aatttcataa
    24121 ctactatttt aggagatgat tttatagaat ccagagtcta cctgaataaa aaacaaacct
    24181 gtgcatttct aggaatcgat aataatactc tagataagtg gattcgtata tataatttcc
    24241 cagtaataag agttggtggt gtagttcgct ttgacgtaga agatattaaa gactttatga
    24301 atacatttaa aataacttga aattttcttc tcgccgcata tactaaaatt agtgaaatat
    24361 actgccttac tttttttaag gagtgaggat tttgcctaca aagctttcaa atgggaaata
    24421 taaaactaat ctgagatatc caaaaaggtt taaagaaata acaggaattg catcagaaaa
    24481 gtatcaaaaa acatttccaa ataggcaatt ggcgattaaa gccgaaaatg atatgaagaa
    24541 gaaaatagaa aaagttttga gagaggagaa tgcaaactca ttagaattaa aaggaaaaat
    24601 aacgtttaaa aaattttacg aatctaaatg gcttcctaga tatgaattag gtcaaacaat
    24661 tagatcaaac aggcctccta gtgatataac cattagtaat acaaaagata tatttcggtt
    24721 gcatatttta cctatgtttg gtgaatacgc aatgaattat ctaaatatca atactgagat
    24781 aatatcagat gaattaacca aaaaatcgaa agaatatgca aatataaaaa tcataaaagg
    24841 atatgttcgt tccatgttcg atattgcaga aatactaaat tatatcgaat ttaatcgaac
    24901 tactaaaatt attcaaagta tcactgcacc taaaaaaaat gctttagaag aaaaaagaat
    24961 acaagaaggt aagcaagcac tatcatcaaa agaacttact aattggatag aagcagtaaa
    25021 tgatgatttt aataatcatt tacttacttt tcatgattac acacttttta tgattaccct
    25081 gtatcttggt gatagaaaaa gtgagactta cgctttgcaa tggaaatata ttgattttga
    25141 aaaacaaacg gtccgtctta aacatacttt agataaatat caaagaaaaa agtttacaaa
    25201 aggtcgaaaa gataccgtca ttcaagtacc agaagttgta atgacattgc taagtgaatg
    25261 gaaatcggta caagctgatc aattgttgaa attaaaaatt aagcaaacgt tagatcagta
    25321 tctctttact tatactaaac catctggaga agtaaattgt cctgttcatg ccgattacct
    25381 aaactatcga atcaattcaa ttaaaagaag gcatcctgac ttagttcatt tatctccaca
    25441 caaactccgt catacttatg caactatagc tagacaaggt ggagcagata tgaatcaaat
    25501 ttcaaatgct ttaacacact cagatatttc tacaacaaag atatatgtga acacaccaga
    25561 tatagttgat aaggctgttt ttgaagcttt tcagagagga ttgaataagt gtgattgata
    25621 tgtgttctta aatgtgttct taaaaacaaa agaagcactt tccaaataat tgaaaagtgc
    25681 cagaaacgtt gatatataaa ggatattaac gttttgagaa ttgtgaagct ttacgtgctt
    25741 tcttaagacc tggtttttta cgttcgacca tacgtgcgtc acgagtaagt aagccagcac
    25801 gtttaagagc taaacggaag tcaggatcaa cttctagcaa tgcacgagcg ataccatgac
    25861 ggatagctcc tgattgtcca gcgtatcctc cgccgtttac gttaacaaat acgtcatatg
    25921 cgccttttgt ttcagtaaca gcgaaaggtt gattgattac ttcacgcaag tcagcatgtg
    25981 gaatgtattc ttcaacatct tttttattta cagtaatttt gccagttcct ggtactagac
    26041 gtacgcgagc aactgcgttt ttacgacggc ctgtgccgat atattgaact tgtgccaatg
    26101 aaattccctc ctattagatt aagtttgtga tgtctaaaac ttctggttgt tgtgctgcat
    26161 gtggatgttc tgctccaccg tatacgttca attttgtaaa ttgtttgcgg cctaaagtgt
    26221 ttttaggaag catacctttt acagaagttt cgatcaaacg acgagaattt ttagcacgca
    26281 attcgccagc agtgattgat ttcaatcctc ctgggtagtt gctgtgacgg taataaattt
    26341 tgtcagtcgc ttttttacct gttaatttca ctttgtctgc attgatgacg atgacgaaat
    26401 cgccagtatc caaatgaggt gtgaaagttg gtttattttt accacgtagt acagatgcaa
    26461 caactgttga aagacgtccc aatggaacat cagttgcgtc tactacatac catttacgtt
    26521 ctacttcgcc tggtttggcc atatatgttg tacgcacgtt attttcctcc aatttaagtt
    26581 ctgatgtttg acatacactt cgagtttccg gggctctttg tggggcaaac aataccattt
    26641 aacatattac ccgctaatct agtcaatgtc aacgtttttc tgcttgattg aaaaatttat
    26701 atgaaacttt acttcttttc tttcttttat ataaaagggt cgtttcctgt caaaatgaaa
    26761 aacaaaaaga ggctgtgaca aaagtcttgt cacaacccct tattttgaat aaacggtgtt
    26821 ctcctgccaa ctcctcggtc acaagtcaca atatccataa aacataagaa gctgtttttt
    26881 aaaattgttt cttgtgactc ggagctgaac ggcaggctta caacctcttg gtaaacggtg
    26941 ttcaatcagc tgaccctcgg tattagctca aaaaacggca aaatatgagg aactattttg
    27001 acgtttgttt ctctaatact caggtcaaaa cgctgatttc acaacctctt tttcggtcaa
    27061 gttcaacaac agtttttacg cgtcgatctc tggcgctttg cccaaatgag cttggatcat
    27121 ccagatacgt ttttctgtgg ctgttttgaa ttcgataaag atatcttgtg ttggatcgtc
    27181 gccttcttct ccagaaactt cgattccttt ttggtaaaga tctgctagat aacgatagcc
    27241 tgcaacaagt gtttccaaac gttctggaat agtacgatcc cagcggccaa tttcatcagg
    27301 gatttttgtg ttatctgcaa attcacgcaa tgtagaataa ggtgaaccgt ctaatgtgat
    27361 caagcgttcg gatacttcat ctaactgagc gttcaaatca tccatgaact catccatcat
    27421 tgggtgcaat gtaaggaaac ctggtcctct catgtaccaa tgtgtttggt gaacaacaac
    27481 agacatttgg cttagatctg caactaattg atttaatact tctttggttt gttggaattt
    27541 catctttctt gcctcctaac attttcttac acttaaaata atagtagact ttcaatgaaa
    27601 agtacaacaa aatcatttac ctgagaacga ttttcagttc gcttttttat ccgtaaagcc
    27661 aatcattttt cagaatcagc taattcttct cgcagttgag caaattgctg ataagtctgt
    27721 gctaaatgct tttgtgtcga aacaaataat ggatagattt tctgatattt tttatgatca
    27781 gtttcttgta ctccaacttg ctctttcaat tctatcaatt cctgtgcttc actgatactt
    27841 tggatctttc cgatgcttct ccagcctaaa atcacagctc ctaatgcgga tgcttctgtg
    27901 acagtaggga aaacaagcgg gtaacccagt acatcagcca tcatctgacg aaaaatcgga
    27961 gattgagcaa acccgcctgt agcacggatc tctgtgagct gattttcctg gcaaactcct
    28021 tttaaaatcc gataaaggtt gagacagatc ccttctagaa tcgccttcat catttctttt
    28081 tctgtatgtt gccgctgtaa accgataaaa ctgccaaacg catctgcgtc ccaaagcggt
    28141 gctcgctcac caagtaaata gggatgaaaa atcagaccat tggcacccgc tggcacaaaa
    28201 gcgatagccg aaagcagtgc atcatatgga ttttctcctt tttctttagc agattgaacg
    28261 acttcctgta aaaatgtatg ccctgcccaa tcaaagatgc cagcaccatt gctagtcgct
    28321 cctcccacta cccaatgttt ctgatccaga acataacaaa acgtctccat ttctggatgg
    28381 aactgaggct gagtagtaat aaaacgaagt gctccgctcg ttcctacggt caaagcggcg
    28441 atcgtatcac caaaagcacc taaccctaga ttagaaagcg gcccgtctgc ccctcccaaa
    28501 acaaatgata tatcatttgg caaacctagt tctgctgctg tttttgatgg tagacctgtt
    28561 acttgatagg tggaaggaaa gacttgcggc aattgctcta ttcgaatgtt caagtagtca
    28621 agcacttctt ctgcccattg gaattcatgg atatcaaaat atccagttcc tgaagctgtt
    28681 ccgtagtcgc tgatcagttc gccaaacaat ctataaaaga tatagtcttt gataccgaca
    28741 aaataagcag tttttttgaa taattcaggt tggctgtttt gcagccactt gattttgttc
    28801 aatggtgtca ttggatgaat cggcgtccca gtctgccgat aaaattcttt gccaagcacg
    28861 gtatatttca gtttttctgc ttcgtccgct gctcggttat ctgcccaagt gatcattcga
    28921 gataacggtg ttttctcttc atccaacaat atcaggccat gcatcgcaga ggagaaggag
    28981 acaaattgaa tgattgtgtc tgaaggcaac tgtttaatta cttcttttat aacggtgcag
    29041 acaccttgaa agatcaattc ctgatcttgt tctgccatcc cttgtgtttc ttggatcaaa
    29101 gggtagcgaa aataagcgga tgctgcttct tgggcttcat ctgtgaaagc aactgctttg
    29161 gtttgtgtgg ttccgatatc taatccgatt gaaacgttca ttttttcacc tcaaatttta
    29221 tgtatgtaca aaaatttagt ttaagcttgc gtatcttggt tttgaaaagg gggagatggc
    29281 gtgttacttt attttatcat tggttgctgt atcggatcat ttttatgttt aaccgcccag
    29341 cggattccgt taggtcactc tattatttat cctagatctc attgtgtcaa atgctgtcgt
    29401 tcgctttctt ggtatgaatt gattccgatt ctctcgatca tcgtccaacg atttcgctgc
    29461 cgctattgca ggtgtcggtt gcccttctac tatttgttgg cagaagcctt atgtggtggt
    29521 ctttttgcct ggttctttac tttttcttcc gcaagaaatt tttcgacgtt catatggatg
    29581 ctttcggctt tgctattttc gttgatggat ttattttact tcatggtcca ttctcacacg
    29641 ctattctgtt cttgggcaat tttatggatt ttttggttgc aggccggcgt cttccaatgg
    29701 caaagtgtgc tgttaacgtt tatcgtaggc ggtgtgtgcc ttcgctatgg tcaatcctat
    29761 ctcggagctg gcgatactct gttgatgctt tcttggagtg gtggcttgtc tctagaagag
    29821 ttgcttcaga tcttgtttct tgctagtctg tttggaatat ccgttttttc tgtttacttt
    29881 atctgtcata aaaaaaagtt ggaaaagctg cctttcattc cttttttgag cagtgcgttg
    29941 ctgattgtcc ttcatcttag gtattgaaaa aaggttgtga caaacgactt gtcacaacct
    30001 ctgatatata gtgttctctt accaaatcct tgtgacttgg agctaggcga caggttcaca
    30061 acctctccac taaatggtgt tcaatcagct gaccctcagt attagctcaa aatgctggtt
    30121 ttacaacctc tgatatacgg tgttccataa gtagcttttt cggcttctta tttcttgacg
    30181 ccccaatgtc gacgaaggaa acctgcacgt ccagaacctt ctcgataaag attgtaatgg
    30241 agcttgtttt tcttgtaata atcctgatgg taatcttctg caggataaaa ttcgcttctt
    30301 ggttcgattt ttgtcacgat tggcttatcg aatcgcccgc tgtcttgcaa tgcttgtttt
    30361 gaacgctctg caatatttcg ttcttcttca tcattatagt agatcactgg acgataagaa
    30421 tcaccgcgat cagcgaactg tcctaatgcg tcagtcggat cggtttgctg ccaatagatt
    30481 tccaccaact gatcataaga aataacttct ggatcatatt cgatctctac tgcttctgta
    30541 tgccctgttg tgcctgttgt tacttgttca taagttggat ttggtacatg cccgccagta
    30601 taaccggatg tcacagaaat gatccctggc tgctcttcaa atggtttgac catacaccaa
    30661 aagcatccgc ctgcgaaaac tgcttttttt gtcatataaa atcccccttt tttctcatca
    30721 taccttgatt tccaaaagga ggataacgtt ttgcttgtga cagaaaaaac ggaaactatt
    30781 atatacgtga tacggcttct gataaatgcc aaaacgaacg tacttgatca aaaacggtta
    30841 aatcaggaat cacatattca gcagaaggaa gatttatgtt ttcacctatt cccaatacat
    30901 gcatgttacc agccaaagct gcttgacaac cagcttctga atcttcaatg accaagcatg
    30961 catttggcgg tacattcaat tgctgtgctc ctttgagaaa aacttctgga tcaggttttg
    31021 cttttgatac ttgcgtgccg tcaataagga catcaaaata acttgtcaaa ttcgtttttt
    31081 ctaaaatgag gcgtgcattt ttgcttgctg atcccaatcc gatcttgatg tttgtttttt
    31141 tcaagtactc aagaacagct attgcaccag gcaaaacgga tgtttcatcc attttttgaa
    31201 tcgcttgaag atatagattg tttttttgct cagcaaaggc tgatttctct ttttctgaga
    31261 atttctgttc catttttcca aatgacagca atcgctctag tgactcggtt ctggatattc
    31321 ctttcagttg ctcgttttct ttctcagtga atggaatgga cagctggtta gctaaaacca
    31381 gccacgcttc gtaatgataa cgagctgtgt ctacaagaac gccatctaaa tcgaatagtg
    31441 ctgctttgat tttcatcgat tacctccttg aaaatgaacg acttgaagta aagatgcgat
    31501 gtttttagct gtgcgatgaa gattatcagt ggcttgctgt aaagcttctt gtaaaggcac
    31561 agctgcagga aggatcggaa aaacggccga aatgccaaca tctgctgctt tttcgcttcc
    31621 tttaccaaca cttccgcaaa tagctatcac aggaacgccc tctggtactc ttctagcaat
    31681 cccaactggc gcttttcctt ttagactttg tccatctaga cgtccttcgc ctacgattac
    31741 gaggtctgct tgctgtaccc gtttatcgaa ctccagtaaa tccaatacgt agtcgatgcc
    31801 agagactatt ttccctgaag caaatgctaa cagtcctgca cccattccac caccagcact
    31861 actgcccgat gcctcgatta cttgaggggc aaatctcata taaaaatcat gcatggcttg
    31921 gtcagccact gctaattcat tttctttcaa gcctttttgt ggtccgaaaa caaaagaagc
    31981 gccttctttt ccgcataaaa gattagtaac atcagtaatg acagtgattt tggctttaat
    32041 cagctcaata ggcacgttcg ttttatcaac tgtagcaatt ttacctagat tttctcccag
    32101 tgcttctact ttttgattat cttgatcgta aaaagcataa cctagacctt ctgccatgcc
    32161 gatgccgcca tcgttggtac tacttccgcc aacgccgatg atgatgtgtc gaacaccttg
    32221 ttgcaacaat tgtcgtatca gttctcctac tcctcgcgtg gataacctaa gtggctgttg
    32281 ttggtttagt gggacatctt tcaatccgac gatttctgcc atttcaaaaa tcgcagtttg
    32341 accgtctgaa gcgtactgga tttctttgtt tttgccaaat gcattagtaa cgactgctgt
    32401 ttgccgcttc aaccttaaac tgtctgctaa tgatgaaacc gttccttctc ctccatcgcc
    32461 tataggtagc agatcatatt cagcctgaac aaaaacttct tgaaaacctg attgaatggc
    32521 ttgtgctgcc tctaaggctg tcagactttc tttgaaagaa tctggcgcaa tcagaatacg
    32581 cattattttc ccctccgttc caagatcagt tctttttcca gctggtattg gtctccattg
    32641 acaaaaatag gtaatgtttc tttcgtctct ttttttatta ctatttgttt ttttgtcaaa
    32701 acaaatagca acctgtgatt tttccaaata aaaggaaaag aaattttttc ccatgctgaa
    32761 ggaagtcttg gagaaataga aagagttcca ttcgttactg taatacctga aaaaccaaat
    32821 ataactgcta gccatagtgc tcctaatgaa gcagcatgaa ttccgtcatc tgatgaatgc
    32881 ggatctggtc ctaaatcaat caggcacgct tcttggaata aatgatacgc ccattccatt
    32941 tcttggatac ggcaagcctc tatggcgtga atcgctttac tcaacgatga gtcgtggatt
    33001 gttttgtttt catagtaatg caaattctta gaaacgattg gttgagaaaa atactcaggt
    33061 aataagtata acagcatgac aagatctgct tgcttgctga cttggagttc atttacttcg
    33121 ctacgggcat aatcaagtaa aatagactgg ctgccaggac gttctttgta agtggtcaaa
    33181 tcaataactg gcttactgag atacgtgtca tcttgcggaa tcagctgttc tttgttaggc
    33241 actggcaaat ataacttatc taaaaaattt tgacattctt tcaaaaaaag cggatcgcca
    33301 gcttgatatt taacagctaa ggccacatta taatgcgcca gatagttcgt atatgcatta
    33361 ttgtcgatat attctgtgta ttcatctgga ccaatgactc tgtgtatctc taaatgatca
    33421 tcgactttta cagctctgct cagccaaaaa gctgctgttt catgaatgag cgtttttccc
    33481 tttgtttcta aaaacgattg atcctctgtt gcttggacat actcttgaac ggcataagca
    33541 aggtctgcac tgatatgatg ttctgcggca gctgaagcaa cttcttgtct tgttccggtt
    33601 ttgatattca atgcagcaaa tttgggtgtc tcttcctgac cagtgtgtgc gctttcccat
    33661 gggaaaagtg caccttgata gtgataatct cttgcttttt gccgtgcgcc ttctaatcga
    33721 tcataacggt acagcagcaa attctttgct acttcaggaa actgatataa aaagaaaggc
    33781 aggataaaaa tttccgtatc ccagaaaaca tgccctttat aaccttcgcc agttagtccc
    33841 ttcgccccta cactaacact tggatcattg cggggagtca taatctgtaa atggtaagca
    33901 gcgaagtcca tcgctaactg atcgaaagaa gaatcgcctt ggatcgttat ccgatgctga
    33961 tgccaaaaat ctgtccaata agacatcgac tgctctaaaa tttctgtata actaacgctt
    34021 ttctgggctt gggcaatact tttcttaaac gggtctgttt gctgaagaga ggtagagagc
    34081 acgatgatct tctccagcgt aaaggacgta ttttttctga tcttttctga aaaggaagcg
    34141 gtgatccggc gattttttgc ttggaataca cctgctttat tgaatttagc tgcaatccca
    34201 atatcaattt tactttctgt tgtctggtag gatgcactga tcaaatcgtc accaaatact
    34261 cgcagttctt tttcaaccaa ctgctgtgta ccaaaattgg tttgctgtcc atcgataccc
    34321 gttttcacat ctacttggca gtcttcggtc aacggcttga tgaccacttt cagggctgcc
    34381 tgatggagat tattttgatc agcaacacga caagtaatga cttccacttt ttttccttga
    34441 gcagtttcaa tgagcgcata gcgtttaagc tctcccattt ctaaatctag tgcccgctcg
    34501 tatgcaagaa ctttcatcgt ctcaagagaa aaatgttcac cgtcgatttt tatttcccat
    34561 ctgatgacat ctgggagatt cactaactcg gctgattctg ctcctaaagg tcggttatat
    34621 acaccagctg caaacattcc ccttacttga ttccgatagt cttcctcctg gcttcctctg
    34681 ataccaaaaa atccatttcc aatggtcatc tgactcgcat acttattgat atactctaaa
    34741 tcaaactgtt tttcttctac tttcataagg taataacttc tcctgtttct gccgaacgat
    34801 ataaattttc caccagattc tggatgacat atccttgcca gccatctgca atcatctgag
    34861 tctggtcacc gagcgcccga ttcacaaaat gagccatact tagttcatgt gggtcattaa
    34921 ctggcgtggt cttttgtaag gaaaccagct ttccatctac atcttcgaat agttcgcctg
    34981 gatacagatt agcacctgcc tgatcgccac aaaaagccac attcagtttg tcttctctga
    35041 tattcaaagc aaacgaagta ttcagttgga tgatcttgcc gttttctaat tccagtgtgc
    35101 caaaaattgc atcttctact gtgaatttgc ttggatccca tttcccgaat ttccctgaat
    35161 tttttttcgt accaattttt tggaagctgt acgcccgaac gctttttact ttaggaaatc
    35221 cagtaatata catcgctgta tcaagcatgt gaatgccata atcgatcaaa ggcccgcctc
    35281 cttgaagttc tttgtcgata aacgttcccc agcctggaac accagaccga cgaagcgcat
    35341 tcgcttctac gtaataaata tccccgagaa catcaagatg attcttgatc attgttgctt
    35401 cgtcagaaaa acgatgctgg aatccataag ccaaaatctg cccgttttta tcggctgtct
    35461 ctgccatctt tcttgcttga tcagctgtca tagctggtgg tttctcgcac atcactgaag
    35521 cattagcttc gagggcagcc aatacatgtt cgaagtgaaa gcgattgggt gtacagacac
    35581 tcacgatatc gggagaaatc tcattcagca tttgtgtaac tgatgtatac gcatgagaaa
    35641 atcctgtacg tcggcaaaag tcttctgctc gctggctgac tggatcaacc acagcggcca
    35701 attctaagac atctgatctt ttttgataat aagcagcatg tactttttct gctacttgtc
    35761 cagcgcctat gattgctact ttcttcattt atttctctct cctttgctta tttacttatt
    35821 gttattcttt ctctattctt tctctaaatc atttaaatga cgcgctgttt ccagacagtt
    35881 tttcaagtat tgcaatgatt cctgataaat tttcacttca ttttctcctc gcaaacgaca
    35941 ttcatatacc atataacctt catactcatt ttgaagtagc gtttgaaaat ggcgagtaaa
    36001 atcaattgcg ccagatcctg gttggtagcg atgattatct gctaaatgga tatgacccac
    36061 atactctttt tgcgtagtca atgcttttga aatatcatct tcttcaatat tcatatgata
    36121 aaaatcagcg atgatctgca catgtttcaa ttgattagag tcaatgtaat gacgcgcatc
    36181 cgctaacaaa ttgatcatat ggtcttggta tcggttcaaa ggctccaaat aaataaccgt
    36241 acctgtttct tcggctactt tatctaaata acgaagtgat tcgctgactg ctttatagtc
    36301 tccttctttg cttcggggtg aaaccatcgg cggtaatcga taagtgaaca ttccccaagc
    36361 tgctgggata acgattcctt tgcctccaac ctcgcttagt gcacgcaaaa tctcagcaat
    36421 ttgacgtaca ccattaagac gccgttcttc gataaagtca ccgatccacc catcgtagcc
    36481 gttacaagct gtcactactg ggatccctgt ttctttgatt gcctgtttga ctgtgtcgat
    36541 attttctatt aacaacttac cgtcgatttc gtattcttca aatccacagt ctttgataaa
    36601 tctaaatttt tctaaaatgt ctcttgggaa aaatgcttga tcttgcgtac cgaatttcat
    36661 tgttctcctc ctcggacttg aacgcccatt ttgatacttt cttctgggtg ctggtctaca
    36721 taacgcatgt aagcttctgc gctttctgca aatggcacaa ctggatcaat gatttcttca
    36781 caatccaagt aaccgttcat caataattcc caacatgtgt cctcgattcg tttacggttc
    36841 catcttgggt aatctgggtt tggttcacta gatgctcgag caaagacgat ttttgcatta
    36901 ttgaaatgtg cttcgcgacc aagattaaac ccagctggaa aaggtttagc aaaggcaaca
    36961 taagaaatga tcccgccata gccgatccct attaaagaag cttgtaatgc ggcagcactg
    37021 ccactcgttt caataactga atctactcca cgtttatctg tcaattcctt tagacgaaaa
    37081 ccgacatctg ctttcatagg atctagcgtg taatcggcac cgtgtttttc tgctaattct
    37141 ctacgctgcg ctatgggatc gatcccaacg atggtagtag cacctgcttt tttagctaat
    37201 tgaatcgcaa tttgcccgat agcgcctaaa ccgaagattg ctacagcatc acctgctcga
    37261 acatgtgctt cccggatacc acctaaagcg aattgagcag gatcgtaaca tactgcattt
    37321 ttccattgat ccaaccgatc aagtttacgt agtttataat tatcaacacc gtttgtaatc
    37381 agcgtttcac gaatgggtcc ataagtgcag actaactctc ctttttgata ctctgtcacc
    37441 aaactgcccg tttcttcgat cgtaccgaca atcatatttc ctaattgaaa ttcgccaaat
    37501 accacaccgc gttggcttcc ttcttctctt ggcaagaata ggcgccattc ttctgaaaat
    37561 tcttcatcga taaatggact taatcctcta aagtcgacaa tttctgttcc tggttttgga
    37621 gtagcgaaaa ttactcggat acgtacttcg ttcttttcga tctcacggtc ttcgtaatct
    37681 ttgaatccgg ctattcttgg ttctaatgca actaattttt tcatcttttc tttcctcttt
    37741 cttttctttt tatcctttta cgccgccttc gactaaatca cctttaataa atcgttcaga
    37801 taacgcatac ataatgatga ctggtaaagc ggtcaccaat gaagcggcca tcattcgtcc
    37861 ccaaatgtag tctggtgtac tgaataatgt gttcaaaccg attggtaaag taaatttttc
    37921 ggaggtcgac aggaaaatcg aagcaaataa ataatcattc cacgaaacca taaagcaata
    37981 aacgaagaca gaaatgattc cagaaatagc caatggtata atgatccgaa taatgatctg
    38041 gatccggcta agaccatcga tcattgctgc ttcttcaata tcagtcggaa tcgtgtcaaa
    38101 ataacttttt agcatgaata tcgcagtagg aagtgtttga acgaccatcg tgataatcag
    38161 tgctgtattg gtatcataca aacctagacc ggaaatgatt ttaaataatg gaacaacgat
    38221 caaaataccg gaaaacatat aaacggtgta aaagctagca ttgattttcg agcgagcttt
    38281 gaagcgtagt tttgacaaag cgtatgcccc gctgatccca atcactactg cagttacgga
    38341 ggctatcaag gagatcacta aactattttt gaaatacatg atgaatggaa aaatctcagc
    38401 attgaaaata tcctgataat gttcaaaagt ccattctttc ggaaaaatag tttgtgtcgt
    38461 tgcaattgct tcttgtgaat ccttgaatga tgtcatcagc atgatcaaaa atggaaacaa
    38521 attgacagct aatagcaaca aaatcgtcgc gtaaaataag attttcctgc tctttttatt
    38581 tttcatttca agtccacctt ctttcgtgag agcaagacga ccgctgcaat aatgatgaac
    38641 aatagaatcg atatagctgc tgcttttcca agatcattga acgcaaaggc tgtctgatac
    38701 aaataaacgc ctaagatatt gactttattc gtcagtagat agacatctgt aaacatatag
    38761 aacatccaga tagatcgtaa aacaatcaca gttgctagta ctggtttgat tgccggcagt
    38821 gtcacgatct tgaacttctg ccaagcattt gcaccgtcca tttcagctgc ttcataaagc
    38881 gttttgtcga ctgtttgcaa aatagccaaa aatgaaataa acgcatacgg atagtatctc
    38941 cagatagcaa acaaaacgac cataaagaag ctagcaactg gctggtcgaa ccacagcggt
    39001 gcttcgttga aaagctgcaa cctatctact aacagatcat tgacgattcc ataactatta
    39061 ttgaacatat atttccaagc aaaaatcaaa gaaatagatg gcgtaacata acttaataaa
    39121 atcagtgaac gcaccgtttt cctaaatctg aacttgcggt taagcaaaat agctacggct
    39181 aatcctaaag ctgtactgcc tgctacaact aagaatgtgt aagcaactgt taaacctaaa
    39241 gaacgataaa attcactatc acttaaaatt tcaagataat tgcttaatcc gataaaaacg
    39301 gcgtctgagt taggattcag cggcatatcg aaaaaactga tctgaatatt ggaaagcatt
    39361 ggaatcagca ctaaaacagc taataaaagc atgcttggtg ctaaaagtcc catggctaat
    39421 tttaagtcag aatgtttcct tggacgattc attgaaacct ccccctgata atctgccaat
    39481 agctatctac ttttttcgaa caccattggc agaacttctt gttatttcgt atttttttgt
    39541 gcgtcagcta aagcttgatc gacatcttcg tttccgacag ttacttggtt cactgctttt
    39601 ccgatcacac ctgagcttgt gatatctccc attttcgtga aattcttttc cccgactagt
    39661 ccaaagactt gtacttcatc aaaagcacta gcaatctcag atggcagctc gccaaaagac
    39721 ttgatgactt cattttcttt gtaaccatcc agttcgacta cttgtttatt cactggttga
    39781 gcaccaccag gagacatcaa gacccacttt tccatgtttt tcggttgaga taagaaagct
    39841 gtgaatgctt cagcagcctc tttttgtttc tcgtctagac cagaagaaat cgtcaatcca
    39901 gatactgtgc catagacagc ttgatctttt tgtgttggga tagcaaaacc gatattttct
    39961 gagttccccg cttcataaac agaaggcaaa atgtaagttg aatagattgc cattggtgct
    40021 gttccattca tgaaagcatc tttgatttct gtcacatcat tagagcctgg catcgtgtat
    40081 tgggataagt tttgataaaa cgacaatgct tctctcatct tttccgtatc gatcgttacc
    40141 tcgccttcat catccaaaac atttgctcca tttgacaaag caaattgaga gaacgcttgc
    40201 tcactcattg ttccttcagc agtaggcata gcaattccat attgtttatt ctctttatct
    40261 gtgaaatgtt gagcaatttt taaaatatca tcccaatttt ctggttctga aaaaccagct
    40321 tctgataatt tttctttgtc ataccaaata ccttgaaccc agccgctaat tggtgcagca
    40381 atatagcttt ttccatcttc tgaacgaaca aggtttttgg ctccgtcata ataattatct
    40441 tctccagctt cctcgataac gttttggact gccgtctgat caatcagttc atctttatcc
    40501 atcactttag caaaatcctg actgacttcc attacttctg gtaatttacc ggaacgagct
    40561 aacgtaacga tttttgtatt gaacgctgat tcttctaccg gcacttgctt gattttgatg
    40621 tctggatttt ctttttcaaa atcagcaacc agttcattga tcacacttaa tcgttcctgt
    40681 tcgacagaag agtgcatgaa ttgaatcgtt gtctgttgtg attcgttaga gctttgactt
    40741 tcaccacaag cagtaagaag aaatccagta gctagcagac aggtagtaac aataattttt
    40801 ttcatttttt tccacctctt attttattat tttcagccac atatattcat acggctggaa
    40861 tatcaacttt cgttcttgac tgcaatggat aatttgtcca gaaaacaggt ctaggtaatc
    40921 tccttttggt aaaagataac ctgctgattg attggaaaaa ttatggataa ctaagacttt
    40981 ctcttccttc gcttcgcgaa taaaactgat aaaagaatct gctgagtcca gtaagtaaat
    41041 caaactattc ggatggaagg ctttttcttt ttttcgaata gatagcatct gggtcaattc
    41101 accatatact gctgttctta atggatctgt ctgcaactct ttctcaatat ctgctaacaa
    41161 aaatttttgc cgattgattt ttcgattctc tcctgttttt tctacgcctt tgtaatcatt
    41221 tcgacttccc agcacacttt gaatataaat tgccggcacg cctggtaacg ttaccaaaaa
    41281 tgaatgagca gctaaaaagc gtttgcttct ccattccgtc gtatctgttg gagaacttaa
    41341 cgcatctaga tacgtagcgt ttatctcata tggactggtc gttccatctg ggtttttctt
    41401 gtagtttact cgggcaccct ctttcttcag ctgttccact aattcaagga tcgttttttc
    41461 cggcagaata cctcgtaccg gattcaatcc gataccgtca tgggaggcta aaaaattaaa
    41521 aaaagttgtt tttttcgatg cgaaatcgat ggtttttaac cattctttca acttacttgt
    41581 attacccgaa cgaatcgctt gaagggtcaa aggtggtagc gaaaactgat agaccatctg
    41641 tgcttcattc tcgccattcc caaaataact gatattatct ttgtgtggca catttgtctc
    41701 agtgatcaat accgttcctg gagctgcttc atcagcgatt gcacggaata attggatgat
    41761 tttatgcgtt tctggcaggt gtatacaaga agttcctagt tctttccata aaaatcccac
    41821 agcgtccaag cgaataaatg aagcgccttt ttctaagtaa aacaaaagaa tatcgatcat
    41881 tttcaataaa acttctggat ttttaaaatt caagtctact tgatcttcgc taaaagtcgt
    41941 ccatacgtgt ttttctttcc cgtttttgtc aatgaacgtt gtaagtaacg ggctggtcct
    42001 tggtctagtc acgctgcttg tatctgtatc cagtggcaaa tcaataaaaa aatctttgta
    42061 cgtatcattc aagtttaaat attcctgaaa ccaagtactt tcggcagaca tatgattgca
    42121 gacaaagtcg aacattaacc gtgtttcgct tctcatttct tctatatctt cccatgtgcc
    42181 caaacggtcg ttgattgcct gataatttac aacggagaaa ccatcatcag atgtatatgg
    42241 gaaaaatggc aagaaatgta caactctaaa tgtatctgcc aaatactttt gataaaattt
    42301 cttaaatgta cgcaaagttg gttgctgctc ttcaaaaaat tgatccccat aagtaatcaa
    42361 gaaaatatct tcttcgtccc aatgcagttt gcctgcctgg cctttcttct gctgataaac
    42421 actgactttt tgttcaatca tagaggcggt tttctcagac aaatctgagt cgcgataaat
    42481 cgcttgtaaa agttttgtat acatcggatt cccctttctt ttttgggagc gttcccaacg
    42541 atttgatcat attatggaaa acgcttactg tcaatagcat ttttttaaat aattttgata
    42601 tacacaaaaa tacaaataag aaagcttatg tatcaataat taatagcttc ttaaaaaaat
    42661 atggtctctc atgatcatgc aagctattcc taaattttca aaatcataga caaaaaagaa
    42721 tgtctattgt actatggaat tattctaaaa cgctcccaaa aaggaggtgc atataaaatt
    42781 gaaacctact atttatgaca ttgcgaaaaa agctaatgtc tcaaaatcaa ctgtctccag
    42841 agtattaaat aaccagccaa atatctctga taaagcaaaa gcaagtgtac ttcaagcaat
    42901 caaagaattg aattatgaac cgagcaaaat tgcaagagga ctgtcaggaa atggttttga
    42961 cgcgattcta gcattatctc atcgtacttc tcatacgacc tatggcaatc cttttttttc
    43021 agatgttatt caatccattt ctgccgtttc tgaacaaaat aacttcgact tgattctaca
    43081 aacggcaaaa acaccagaag aagagctaga aaaatgcgcg aaaaagatcc aagaaaaaat
    43141 gatccgagga attattattt taagctctac gatggatgaa acacttcttt ttgaattgga
    43201 tcaatataat attcctattg tggtaatcgg aaaagtcgga aacgactaca cgaacatcta
    43261 ctcagtggac acggataatt tccaggatag ttacgatttg gtggaaacac tgatcaagaa
    43321 tggacaccgt catatcggct gtctccatgc tccgacagaa gtttatgtat ccaatgatcg
    43381 tctcaatggt taccgtcaat gcttatttga tcaccaccta gatatccgta atgaatggat
    43441 tatagacggc ggctttcgct tagaagatag catggcagca atgaatgatt taatgaagct
    43501 acgagaaaga ccaactgcga ttttttctac cgatgcatta aaatcattga gtatttacaa
    43561 agtggcagaa caatctggaa agcagatacc tgatgatctt tcgatcgcag gattcagtga
    43621 tcccttattg tcctccctat tttcgccaca gttgactcgg atagacattc cgacaaatca
    43681 gttaggaatc aaagccacag aacttttatt ccaactaatc caccaccaat accctgaaga
    43741 aaaaagaatc attctttcaa ccgagattac tctcacagat tcgatccaac aacaagaaat
    43801 gtagaacaga attataaaag aacagaaaca ctgaggataa gtcagttact ctgttctttt
    43861 ttcaatcact catccctcgt taccccctcc cggtatttta gcgaattttt cttattttcc
    43921 atctgtttta gatagctgtg gataactata atgtttgtgt taaactattt tttccacagg
    43981 gaagaaaaaa ctatttttaa ccacgttgtg tataagtttt tatagttcgt ttttttaaac
    44041 acatttaccc ttatttcaat ccatgttatt tggggatact tatctctaaa acataaatag
    44101 aataacagac atgtctactg gttttgtttt attcgaggaa ttattcaata atagctagca
    44161 aaatattaaa aataaccgct ctatcttccc atcaatttat tttgcattca aaaaatgatc
    44221 tatctttact tttgttgatt gacttctctt ggaagatgat cgttccgata ccttaattgg
    44281 atacgaaccg atgttgttaa gatcctttcc cacttttctt ctagttagac aaattatttt
    44341 agacaaacca aaaaagaggt tgtgacaata atttgtcaca acctcttttt taaaatatct
    44401 attttgtttg attgttcatt gcgtcttctg cagccatttg cgcttcaata tctgaaatac
    44461 tgtagacatt ttcgcttgct acaccgactt cttttggttc catgtcacga taacgttgca
    44521 taccagtacc agctgggatg atcttaccta taataacatt ttctttcaaa ccaagaagag
    44581 gatctttctt accgcggatc gcagcatccg tcaagacacg agttgtttct tggaatgatg
    44641 cagcagataa gaaactattt gtttctaatg atgcttttgt gatacctagt aagacaggtc
    44701 gagaagttgc aggaacgcct cctgagacta gcgtattgta gttttgatct ttaaagtctg
    44761 cgatgtctaa taatgtacct ggtaagatat ctgtatcacc tggatccatt acacgaactt
    44821 tacgcagcat ttgacgaacc attacttcga tatgcttgtc gccgatttct accccttgca
    44881 tacggtaaac acgttgtact tcacgaagca agtagttttc aacagataat acatcgcgaa
    44941 cttgcaacaa ttgttttgga tcgattgatc cttctgtcaa tggtgcacca cggtggatca
    45001 tatcgccttc tgctactttc atacgtgctg tataaggaac agtatacgta cgtgtatctg
    45061 tttttccttt gattgttact tctttggtac gtgttgcagg atcttctgag atatcgatga
    45121 catcccctgt tacttctgtg atcactgctt gccctttcgg attacgtgct tcaaagattt
    45181 cttggacacg aggaagacct tgagtgatat cgtctccggc aaccccacct gtatggaatg
    45241 tacgcatcgt caactgagta ccaggttcac cgattgattg agcggcgatc gtaccaactg
    45301 cttctccgac ttcaacgtca gagccagttg caaggttgcg tccgtaacaa tgtttgcaga
    45361 caccatgttt tgtattacat gtgaagacag aacggattgt cactgtttcg acacctgcat
    45421 caacgatttg ttttgcgatg tcttcggaaa tcaattggtt tcctgcaatg atcaattcat
    45481 ttgtttcagg atgacgtact tctttacggg tgtaacgtcc cactaaacga tcttctaatg
    45541 attcgatgat ttcatttccc tcgcggatgg cttggatttc aagacctcgg tcagtaccgc
    45601 aatcttcttc acggatgatt acatcttggg ccacgtcaac cagacgacga gtcaagtaac
    45661 ctgaatcggc agtcttcaag gctgtatcgg tcatcccttt acgagccccg tgagttgaga
    45721 taaacatttc caagacggat agtccttcac ggaagttcga aatgattggc aattccatga
    45781 ttcgaccatt aggagcggcc atcaaaccac gcattccggc aagctgtgta aagttggaaa
    45841 tgttaccacg agctccagaa tcagacatca tgaagattgg atttttcgct tccaaacctt
    45901 ccatcagttt ttgttggatg ctgtcttttg cagcattcca aacagcaatg acacgttcat
    45961 aacgttcgtc atctgtgatc agaccacgac ggaattgttt tgtaatagtt tcaacttgtt
    46021 tatgggcatt ttcaatgatt tcttgttttt cattcagtac actgatgtca gcgatcccta
    46081 ctgtgatacc ggcatgtgtc gaatgtttgt accctaagtc tttcatacgg tcaagcatct
    46141 tagaagtttc tgttactttg aatcgtttga agacttctgc gatgatgttt cctaagtttt
    46201 tcttcttgaa cggtaatact aattcttgct ctttgatatg tgcaggaata tcagtaccag
    46261 cttcaacaaa gtatttgtcc ggtgtttgga cggtcaagtt gaagtctgtt ggttcattca
    46321 gataagggaa ttcaggaggc atgatctcgt tgaagatgat cttaccaact gttgtgatca
    46381 tcgtacgttc tttttgccat tcagtaaatg gtttttcacc taatgagtta gggttcacac
    46441 cgatacgtga atgtaaatga acatatccgt tgcgccatgc gataacggct tcgttttgat
    46501 ctctgaagac cattccttcg ccttcgcgtc cttcttcttc cattgtcaag tagtagttac
    46561 ctagaaccat atcttgagaa ggagtaacaa ctggtttacc atctttaggg ttcaagatgt
    46621 tttgagcagc taacatcaac atacgtgctt ctgcttgtgc ttcttcattc aatggaacgt
    46681 ggacagccat ttggtctcca tcgaaatcgg cattataagc ttcacataca agtggatgca
    46741 aacggattgc gcgtccttgt actaaaactg gttcgaatgc ttggatacca agtcggtgaa
    46801 gcgtaggtgc ccggttcagc aatactgggt gttccttgat tacgtcttct aaaacatccc
    46861 atacttcgtc ttcttgacgt tcgattttac gtttcgcatt tttgatgttt gaagctagtt
    46921 cgcgttgaac aagctcacgc atcacgaatg gtttgaataa ttcaatggcc atttcttttg
    46981 gaagtccaca ttggtacatc ttcaagaaag gtccaacaac gataacggaa cgacctgagt
    47041 agtcgacacg tttacctagc aagttttgac ggaaacgacc ttgtttccct ttcaacatat
    47101 gagaaagaga tttcaatggg cggttacctg gtccagtaac tggacgtccg cgacgaccgt
    47161 tatcgatcaa tgcatctact gcttcttgca gcatacgttt ttcattttga acgatgattc
    47221 caggtgcatt caagtctaac aaacgtttca aacggttatt acggttgatt acacgacggt
    47281 aaagatcatt caagtcactt gttgcaaaac gtccaccttc taattgaacc attgggcgta
    47341 aatctggtgg aataacaggg ataacgtcca taaccatcca gcttggttga tttcctgaag
    47401 cgcggaatgc ttctaaaata tccaaacggc ggattgcacg tgtacgtttt tgtccttgtg
    47461 cagatttcaa ttcttctttc aattcggctg cttctgcatc tagatcaaca ttatcaagta
    47521 attgtttaat cgcttctgca cccatagcag cttggaattc ttgtccatat tgttcacgtt
    47581 tttcgcgata ttcgcgttct gtcaacaatt gttttttctc taaattcgta tttcctggtt
    47641 cgatgactac atatgaagca aaataaatga tttcttccaa agcgcgtggg ctcatgtcta
    47701 atacaagacc catacgtgaa ggaatccctt taaagtacca gatatgtgaa acaggagctg
    47761 ctaattcaat atgtcccatg cgttcacggc gaactttcga acgagtaact tccactccgc
    47821 atcgatcaca aacgattcct ttataacgga tacgtttgta ttttccgcag gcacattccc
    47881 agtcttttgt aggaccaaaa atccgctcac agaacaaccc ttcacgttca ggttttaagg
    47941 tacgatagtt aatcgtctca ggttttttta cttcaccgta agaccagctt ctgatttttt
    48001 cgggagaagc caaacctatt tgcatacttt cgaatttatt tacatcgatc aaaaggggtt
    48061 ccctccattt catttaatgg caaaccgcaa gacggctgat cttcgaccgc cttgcagctc
    48121 atagaccgga ttagtctaaa ttatcttctg cggtttcaaa gttttggatg acatcgtctt
    48181 tttcatcttc cacttgttca gcagctttct tttctaattc tttggcagtt tgctgttcgg
    48241 caaattttgt caaagcatca acagtgatca aatcgtcgtc ttcatcgtcc atgtcgcgaa
    48301 gttcgatctc tgtttcttcg atatccaaga cacgcatgtc cagacctagt gattgcaatt
    48361 ctttcaccaa tacgcggaat gattccggta cacctggttt tggaattggt tcacccttga
    48421 cgattgcttc gtatgttttc acacgtccga ctacgtcatc tgatttgtat gtcaagattt
    48481 cttgtaaagt ataagccgca ccgtaagctt ccagtgccca tacttccatt tcaccaaaac
    48541 gttgtccacc aaattgagct ttacctccca atggttgttg tgtaaccaat gagtaaggtc
    48601 cgattgaacg tgcatgcaat ttgtcatcga ccatgtgagc aagtttgatc atgtacatca
    48661 caccgacaga cacacgtcca tcaaatggtt cacctgtacg tccgtcgtac agaacagttt
    48721 tcgcatcgct ggccataccg gcttcacgaa ctgtttccca tacgtcttca tcacttgcac
    48781 catcaaatac tggcgttgct acgtggatac ctaactgacg tgcagccata cccaagtgta
    48841 attccaatac ttgcccgatg ttcatacgtg aaggcacccc taaaggattc agcatgatat
    48901 cgattggtgt tccatcaggt aggaatggca tatcttcttc cggcataata cgggatacaa
    48961 cccctttatt tccgtgacga ccggccattt tatccccttc atggatttta cgtttttgaa
    49021 cgatataaac acgaactaac atgtttacgc ctggtgacaa ttcatctcca gcttcacgag
    49081 taaagatttt cacatcgtga acgataccgc cgccgccatg tggtacacgt aatgatgtat
    49141 cgcggacttc acgagctttt tcgccaaaga tcgcatgcaa tagacgttct tctgcagata
    49201 attctgttac cccttttggc gtgactttcc ctacaagcaa atcgccatct tggacttctg
    49261 caccaatacg gatgataccc atttcgtcta aatcttttaa tgcgtcttcc ccgacattag
    49321 ggatttcgcg tgtgatttct tcaggtccta attttgtatc gcgggcttct gattcatatt
    49381 cttcaatatg gatagaagtg tatacgtcat cttttaccaa acgacggctc atgatgatcg
    49441 catcttcata gttgtaccct tcccaagtca tgaaaccaac taaaacgttt tggcctagag
    49501 ccatttcgcc ttgttccata gatggtccgt ctgccagagt atcccctttt tcgactttct
    49561 cacccaaatg aacgattgga cgttggttgt agcttgttcc ggagtttgaa cgacggaatt
    49621 ttgttacatc gtatttatct aatgcaccgt tgtcgcggcg tacgcggatt tcagaagcat
    49681 caacgtattc aacgacaccg tcatgtttac ataacaaagc agctcctgag tcatgcgctg
    49741 atttgtattc cataccagta ccaacccatg gtgcttgagg attgatcaac ggcactgctt
    49801 gacgctgcat gttggcaccc atcaaggcac ggttggagtc atcgttttcc aagaaaggaa
    49861 tacatgctgt cgcaacggca actacttgtt taggtgatac gtccatgtag tctactttat
    49921 caatagatac ttctaagttt tcactttgag cacgagccat aacaacgtct tgagcaaatg
    49981 ttccatcttc attcaatggg ctgtttgctt gggctacgat gtaatggtct tcgatatcag
    50041 ctgtcaagta gtcgatttgg tcagtaacac gacctgtttc acggtctaca cgacgataag
    50101 gtgtttcgat aaatccgaat ttatttactt tcgcataact ggacaaacta ttgatcaacc
    50161 cgatgtttgg gccttcagga gtttcgatcg gacacatacg gccatagtgg gagtagtgaa
    50221 cgtcacgaac ttcataaccg gcgcggtcac gagtcaatcc gccaggtcct aaggctgaaa
    50281 gacgacgttt gtgtgtcaat tcgccaagag ggtttgtttg atccatgaac tgtgacaact
    50341 gagaagaacc aaagaattct ttgatacttg ccacaactgg acggatattg atcaattgtt
    50401 gaggtgtcaa tgtttcagta tcttggatcg acattctttc acgaaccaca cgttccatac
    50461 gtgctagccc gatacggaat tggttttgta aaagttcacc gactgaacgg atacgacgat
    50521 ttcctaagtg gtcgatatca tcgacattgc cgataccttc catcaagttc aagaaatagc
    50581 tcattgaagc aatgatgtcg gctggacgaa ccgtttttac agctgcttct ggatagccgt
    50641 ttccgatcac gtttacttca cgttcagggt ctcttggtga aaatactttg atgacttgga
    50701 cagtcattgg atctgtgacc acaccgtctt ctgatggata gtaagtcact gaatttaggc
    50761 cattatcaat gaaaggagct agtgtttcca tgacttggtg agtcagaact gttccttttt
    50821 caacgatgat ctcgcctgtt tctggatcaa ctaatgtttc agctaatgtc agattcaata
    50881 gacgtgtttt caaatctaat tttttgttta ctttgtaacg accaacgttt gctaagtcgt
    50941 aacgttttgg atcgaagaaa cgagcattca gcaagttacg tgagctgtct gctgtttttg
    51001 gttcgcctgg acgcaaacgt tcgtaaacgt ctttcaaacc ttcttctgtt cttgagtcgc
    51061 ttgcgttttt atgcaagtct ttttcgactg tattgcgtaa tgtttcgctg tcgccgaaga
    51121 tttcaaagat cgtatcatcg gaaccaaatc ctaacgcacg aactaatacg gttaaaggaa
    51181 tcttacgtgt acggtcgata cgtacataag agatgtcttt tgcatcagtt tccatttcta
    51241 gccaagctcc acggttaggg ataacagtag aaccgaatcc ttcttttccg tttttgtcga
    51301 cttttccatg gaaatagaca cctggtgaac ggactaactg agaaacgatt actcgctctg
    51361 ccccgttgat gatgaatgtt ccttgttctg tcattaatgg gaaatcaccg aagaatactt
    51421 cttgtgcttt gatttcgcca gtctcgcggt ttgtcaaacg caatgttaca tgtaatggtg
    51481 cagagtagtt tgcatcatgc gcacgagctt ctgctactgt atactttggt tctttcaatt
    51541 cataatctac aaattccaaa gatagattgc cattgaagtc atcgattggc aaaatgtctt
    51601 caaacatttc tctaagtccc tcatctaaga accattggta agagtctgtt tgaatttcga
    51661 tcaaatttgg taattccaac acttcactga ttcttgcgaa acttctacgt tcgcgatgtt
    51721 ttccgtattt tactacgtgt ccagccaagc tcttcacccc tctaaaatgt cattcaaaaa
    51781 gactacccga tcaattgtta caataatatt tcaaatatta tttttaggta acaacagaca
    51841 ttttttgcgg cttttaggca aaaaaaaacc aaaaatagaa cattctcttg aaaccttact
    51901 tcgtgagctt ctacttttgt ttccttatat taaagccgaa acggacaata tttcgtttct
    51961 gccttttctt catcagatag tttttgcaac ttcatatact acacttattt tctataaaag
    52021 tcaacacttc ctggcgtttc ttctgctttt tcgcaacatt ttcttaatgt tttcaatttt
    52081 atgttttcaa ttatctgaca attcttaatt gacagacctt tttatctcgg ttatacttta
    52141 gaaaattcat tttgtggagg aacttctatg cgaaccaaca tcaaccaaat cgcaccaaca
    52201 caactgaata acttttactt tagctacttt agataggttt gtattcatca tgatggatac
    52261 aaacgttaaa cgacagtttg tatctatgat ggctaaagaa ctttgtattg cagcaaaaag
    52321 ccacgtagac aaaatgctaa gtggctgagc agtgaaaata caaagtaaaa gtatcaagca
    52381 ttctactttt tcaatttatt ttccgacttt tcaatccatt tggacatttt gtctgaatgg
    52441 attttttgtt tcccaaaatt aaggaggaaa aagaaatgaa aaaaaatata atcatcggat
    52501 taggcgctgc cctgctacta gctttaggag gttgcgcaca aaaagacaat tccgcagagg
    52561 catcagatgg acaaaaaaca attggcgtat tgcaacttgt acaacataac tcgttagatg
    52621 ctgcctataa aggattcaaa gaaggattag cagaagaagg gtataaagaa ggcgataatc
    52681 taacaatcga ttatcaaaat gcccaaaaca atcaagacaa cctgaaaagt atgagtgaga
    52741 aactcgtcaa agaagatcct gatttgctac ttggaatcgc cactccagca gcacaatctt
    52801 tattaaatga aacaaccact atcccgatga ctgtcacagc ggtcacagac ttggaacaag
    52861 cccatttagt cacatccaat gaaaaacctg gcggcaatgt gactggcacg acagatatgg
    52921 tgccaatcga aaaacaaatc ggcttactgt taaatatcgt gccagacgcc aaaacaatcg
    52981 gcatcatgta taacaacggt gaagcaaact ctaagatcca agctgatttg gcagaaaaag
    53041 aattgaaaaa agcgggcgtc aaagtcaaag tactgacagc aaattccaca aatgatgtcc
    53101 aacaagtgac gaaaagtcta gctaaagatg tagatggaat ctatattcct actgataaca
    53161 cgttcgcaag tgcggcaagt gttgtcggtg aagtcgcaaa agaaacaaaa acacctgtcg
    53221 ttgctggcgc agttgatcaa gtcaaagacg gcggtctagc aacagtagga atcgactacg
    53281 aaagcttggg caaacaaaca gggatcatgg cagcaaaaat cttagacggc aagaatccaa
    53341 gtgaacttcc tgtcgaaaca gcgaatgatt tggaactgta tgtcaataaa gacatggcca
    53401 aagcattagg catcaatcca gacagcatca aagaagctga ataaaacaca ggaggattct
    53461 catgacaaat ttgctctctc ttttcttatc atctgcatcg caaggggtct tatgggccct
    53521 acttgcgatt ggtgtatacg tcactttccg tatcttggac attgccgact tgactgctga
    53581 aggaagcttt cctttaggtg cagggatcgc ggcagtaagt attacaaatg gctatcatcc
    53641 attgacagct tgtcttctag gctttttagg gggtgcggca gcagggcttg tttccggact
    53701 gcttcacaca aaattgaaaa ttcccgccct tcttgcaggg atcatcacca tgactggttt
    53761 atattctgtc acatcccgtg tgatgggtgc gccgaacatc tctttattgg gtcagccaac
    53821 gatctttcat tgggtcgagt cattcggcat aacaaaagac aacgctgtac tgatcgtcgg
    53881 tctgcttttt gctttgatcg ttgtatgttt acttgttttg ttcttacgta cagaaacagg
    53941 acttgctatt cgtgcaactg gcgataatct ggcaatgagc gaagcaaacg ggatcaacac
    54001 agataatatg aagatcatcg gttatatgat ttctaatgga tgcattgctc tttctggctc
    54061 cttacttgcg caaaacaacg gctttgctga tttaaattct ggtattggaa cgatcgtaat
    54121 tggacttgct tctattatca tcgcagaagt tttgttacgt aaccagccac tatttctccg
    54181 tctcttgact gtcgttttag gtgcggtcat ttatcgtttt attttagcgt tagtcttcga
    54241 actgaatgtt gaaccaagtg attcgaaact tgcttctgct ttcgtgctag tgatctgtct
    54301 ttctttcccg cagatccagc aaaaattcca tttacctcaa aaaaagaaag gcggtagtgt
    54361 caaatgaatg acgtcttaac attggaaaat ctccatcata cctttgaaaa aggaacgatc
    54421 aacgaaaatc atgtcctgaa aggcattaat ctttccttag atcccggcga cttcgtgacg
    54481 atcatcggtg gaaatggggc tggtaaatct actttgctga acagcatcgc cggtacattt
    54541 tctgtcgatc agggtcaaat tttattgaat ggaaaaaata tcacgaaaaa atctgttgtc
    54601 gaacgttcga aatccatcag tcgcgtattc caagatccta aactagggac agccgttcgt
    54661 ttaacggtag aagaaaatct tgctcttgct atgaaacgag gaaaaaaacg cggcttcttc
    54721 cgtggtgtca aaccacaaga ccgaacattt ttcaaagacc agctggttcg gctaaatttg
    54781 gggttggaaa atcgtttatc ctcagaaatc ggtttgctgt ctggaggaca acgtcaagct
    54841 attacgttat tgatggccac attacagcgt ccagaactga tcttattaga tgaacacact
    54901 gcagcgcttg accctgctac ttccaaaacg gtgatggaat tgacagaaaa gctgatcact
    54961 gagcagaatc tcactgcttt tatggtcact catgacatgg aagacgcgat ccgctatggg
    55021 agtcgactga tcatgctgca tcaaggaagg atcgtcgtag atatccagca agaggaaaaa
    55081 cagcagctca cagtgcccca attattagcg ttgttcaaac aaaacagcgg cacggcatta
    55141 aaagacgatc aagtactttt atcttcataa tcagaaagag gatgggacaa aaaatgaacg
    55201 cccaccctct tcttttttct ctttccttct ttcgtttttg ctttatgtat caaccgttgt
    55261 ttcttcgaaa actattttgt tctctccttc ttcctgatag aatggatggg aaaggagctt
    55321 gatgccatga caacgaaaga caaggtactt caattattga aaaagacacc agattttctt
    55381 tccggagaaa agttggcaca gcaactggaa gtttcccgga caagtatttg gaaagccatc
    55441 aaagaattgg aaaaggcagg ctatcgtttt gagcacgggc ctacagggta tcgttacctg
    55501 ccctctgacg tactagatgc aaaagaaatc cagcaagagc tacggtcgtt tattcctgat
    55561 ttgaaagtac aaacgatgtt gacttctgaa tctacgatga aagatgccaa actggcagca
    55621 gctcaaggag attctgtttc gaagttattt gttgctgata cacaagaaca agctcgtgga
    55681 cgttttggac ggcctttttt cgctgaacct ggacgaggaa tctacatgag tctcttgctc
    55741 catccagaca aacggtttga tgaattgcct caatatacga tcatcgcagc cagtgcctgt
    55801 gtgaaagcca ttgaatctgt aaccggtcgt tctgcttcga tcaaatgggt caatgatatc
    55861 tacatggatg gaaaaaaagt ctgtgggatt ttatctgaag cagtcagtga tatggaaagc
    55921 ggcaggatca gtcatatcat cattgggatc gggcttaatt tttctgttcc tcaaaatcat
    55981 tttccagctg atctccaaga aaaagccatc tctgtctttc ccgacggaca agctagtgtc
    56041 acaaggaatc agctaatcag caaaatctgg caagaattgt tcacttatct ttctcaccca
    56101 gaaaaagcgt tggacttcta taaagaaaaa tcattcgtat tgggaaaaac cgtgtcgttc
    56161 aatcaaaaag gaacgactta tgtcggtctt gcaaaagaaa tcggtgatca tggcgaactg
    56221 atcgtccaaa caaatgaaca gaagatgagt ttgtcttctg gcgaaatcag tctcacatct
    56281 atccgtacgc aataattatc agatttcaca aaaggacacc tgatctcata cagtgtcctt
    56341 ttttctgaat tctcttcaaa agactctcct tattctatca gctgacagtt tctctatcct
    56401 ctagaaaaag gtattctaaa aatatctata aagcatggta cactttcttt aaatgaatca
    56461 atgcaaaaaa caagagagtc atcagtttac acgatcaaaa gagatagcag gccgcttcct
    56521 ttttcaatct tatagataga ccatgggtaa cgataaaaac aaaaaagaac ttgcgactta
    56581 ttttgccgta agttcttttt tagctagtgc cttaatctct taatcccaac aaacgtctcg
    56641 catagatcac ctgaccagag tgcatagcag catcatcaat aatagaaacg agacgcattc
    56701 ctcgtgtgac tggtggtgtc cagttctcgt caattacttc atctagctcg gaaggttcca
    56761 gccttcttaa gtaagaaata gcaaaatcag cagctcctcg caagtatcct agcagatctt
    56821 ctatttgatt agcttggatc tgctttgctt gttccagact atgcttccaa tcttgttcgt
    56881 tatctgctat cgtcatcgga aaccggcttt cccagtcttg actatgccaa attggagaag
    56941 ttcctgctaa atcagcaatc tggaaatcta attctcgagc ggtatgccaa gccagccaag
    57001 tcattgattt gatctgcggg gctctatctt ccactggaaa acgattggcc tcctcaatag
    57061 agacgccttc aaacaaacgt tcaaaccgat cgtatgcccg atccacactc tcgcatagca
    57121 tatctaaata atccatttat tttcctcctt tttatttatc ctacttactt tttcctaatt
    57181 ctaggatacc tacttatatg ttactacaca cccttatttt ttacacttta acagtcacca
    57241 tcatcaatag tttggagatt gaaagaaaaa gatagtgtcg aactatcaat tgaacaagaa
    57301 gcaacaatat gaacgtttca ttaatattct ttaaataaac aatgattttt caaacgaaca
    57361 gtttaagact aggagaaaaa gagtataata ggaaaatgtt tgaaaggaaa ggaaggggaa
    57421 aaatgaaaaa aagggcaagt ttactagtcc tagttgcttt gttgttcaat atcttttcgt
    57481 atggtgtggc cgtttcagcg gaaacataca ttcctaaaga ccctgggaat atttataatg
    57541 atctaactgg taactatggc ttgttaggaa ttgctagtca attccacgta tttgctaaaa
    57601 ataagacaac tgtgaatgca catacagacg gaaatatagc aacacatgaa cttgatgcaa
    57661 aaaataattt tggtacagag cttcatgaag gtttattgaa ccaagaaata aattatatac
    57721 aaaaaactga ttctttcgct aattcttcag gcatacctac aggtagcaca agaatgaata
    57781 aatttgtcgt tggcagtaac gtggatgtag cacttcaaga tggtcatcct acagtcaatg
    57841 gatccaggct tgatcactta actccagatg atttttataa agatcgtgct gggcatgaat
    57901 atattgattt cgagcaggaa tttgctaaat tgaatgtagc agcaaatgat cttgcaacga
    57961 tcactccagc taaaacatat actgctgcag attttccaga catgaacaat cggacaatcg
    58021 atttaactga tttatcagat accgggtttt tattggtaaa tatcgatgca gaagtgttaa
    58081 cgatgaatac gccacttcaa atcattaatc caaatgatca agtagttgta tttaatgtca
    58141 tcaacagttc ttctgcattg aacgtacagt caccaatcaa atacaatgat cgttcaaatc
    58201 atgaaacaga agatttttcg gatgcgaata tctcttggaa ttttggaaat gagatgactg
    58261 atttgacaat cagtgctcct tttcaaggaa caatccttgc gcctaatgct actatccgca
    58321 ttactcaaaa ccaagacgga acgattatag gaacaaatgt gattttagat gcagctacca
    58381 atcgttggga tcctaacgaa atatttatca gcgacaatgg tacagatacg acagacacaa
    58441 gcgacagtac agatacatct accacaagtg atagctcaga ttcatcaact tcaagcgaca
    58501 gtacagatac tacctctacc acaagtgata gcacggatac atcagcttca agcgacagta
    58561 cagatactac ctctaccaca agtgatagca cggacacatc agcttcaagt gacagtacag
    58621 atactacctc taccacaagt gatagcacgg acacgtcagc ttcaagcgac agtacggata
    58681 catctaccac aacaagtgat agcacggaca cgtcagcttc aagtgacagt acagatacta
    58741 cctctaccac aagtgatagc tcagattcat ctaccactac aagtgatagc acggacacgt
    58801 cagcttcaag tgacagtaca gatacatcct ctaccacaag tgatagctca gattcatcaa
    58861 cttcaagcga tagtacagac acgtcgtcta ccacaagcga tacacgtcat gcaagtgtct
    58921 ccttctcaaa aacaagttcg gataacagga cgaactttaa cttaactaat aaaacaagca
    58981 atgacaatgg aaacaaatca aaaagagcac ttcctaaaac tggaagtcaa tcgaataatt
    59041 ggataacact cgcaggcgtt atattacttg taatcggttt aagaatcact tttatgagtt
    59101 ataagaaatc gaaaaggtaa aaaaatattc aagataaaaa agagcaacgt cgctaacgga
    59161 cgttgctctt tatttttgct ttttatcata aatacaagaa ctacaattca atttgtcaaa
    59221 aaacacgtag atttccttct gagaaattta tttacgtatc gaacctttcc ctattcagtc
    59281 tttgcgatcc aaaatcagtt gggtaaggga aaagagattc tcttttgttc gaagagaggt
    59341 ggcaggtaat ttctttattt ccttcaaagc cttttctgtg taagaggccg ctaacttcct
    59401 agcttcttcc acgcctcctg tatgttcaac gattttgcgt acggcgtcga catcttcttc
    59461 tgtcattgcc gctttcttct ctaaataagg tagtagcttt ggtttgtttt tttgcaaaga
    59521 gtagatcaac ggtaaagaat agaccccttg acgcatgtct tctagtacag gtttgccgat
    59581 ttcctggctc tcttttgtat aatctaaaat atcatcgata atctgaaagg ccatcccgat
    59641 agaaagtccg atatctcctg ctttttttgc gaagcgctcg cttgttccac tttcaaaagc
    59701 gccaacagaa caacttaatt ggaacagttc agctgtctta cctgaaatat tttccaaata
    59761 ttcttttaca gaaagttcat aattatagcg atcatccatc tgccctaatt cgccattcaa
    59821 tactttttcc atactgcgag aattcaattg gagacttttc aaggaagtgg agtaatcaga
    59881 caatagttta aaacagcaga caaataaata gtctcctgca tagacagaaa cgacattacc
    59941 aaactgacta cggatattag gctgaccacg tcgcagatcc gcttcatcaa cgatatcatc
    60001 atggatcagt gtggccgtat ggagcatttc gatagaagct gccaaagcga tggctttgtc
    60061 tcgatcttgc tctggtccaa attgtgagaa caatagctga taggcaggac gcaacagctt
    60121 tccgccggca tggatcgttt ctttgatggc ttgttcgaca ggtttgtttt tcaggcggac
    60181 agtttcttcc atccgtttta gtgtttctgt caattcttta cttaaagcag gatagttttc
    60241 ccacattgga tgtagcttca taaaaaaact ccttgtgtga cgattatttt ttggtaaaca
    60301 ttgggctcca tttttgtttg gttccctgct cataatcttt ttggaatcgt tgaagtgtct
    60361 gcacatgtcg taaaagataa ttcgctgcga tcccgattgc aatccctgat aaaatcccta
    60421 aaactgaaag gactggcaag tacaacatga ctgtccatga ttgtgcaatg aaagaagcca
    60481 tcaaaagctg tccgacatta tgcatgaaac cgccggccgc actaattccg atgatactga
    60541 cacgttttgg tcctaattgc ttgaccaata acattccaaa ataactaagc aaagcaccac
    60601 tcatactata aaggaatgtg gaaatcgttc cgcctagcaa agtagtcagg aatagtcgta
    60661 gccaaagtag taagaagctg tcttgttttt tcatggtaaa aatggcaacg atcgtgatta
    60721 gattggctaa gcctagtttt gcaccaggcg caaaagaaaa aggataaggg atcatatttt
    60781 ccaataaacc aatgatcact ccttgtgcaa caagtagcga aatataaatc attttttgta
    60841 atttactcat ggtgtgtgaa ggtctaataa atcagactgt cttcgtcacc tccatctgtt
    60901 gaacggactt cgatcactaa tttatgtggc aaacagacga tggtttcgcc atttttcgtt
    60961 gcccagcctc gacgtacgca gatctggtca tcgcaatctg cttcttttat ccgtactcga
    61021 tcgccatcta tctcgatcaa gttataatct ccgtgttcat ctttgtaagt atatgtgttg
    61081 ctctcttctc cagccatcaa agggaatgtt ttgatttctg tgccatccac acgcagtacc
    61141 gcttcttttt ctgctgttgc ggtgttttgc tgaagaccaa aaatcatgac tggtaaaaaa
    61201 gaaagaacag tcaaaagcaa aataataaaa acatcccaag gtttgagcag acttttttca
    61261 gcaaattctt taaggctcat ttttgttctc ctttttccag attaactcac ccaattttag
    61321 cataaaaaca agaggttgtg acacctgtgt cttgctttga gtaataagaa cgaagaatag
    61381 aaaacagctt tccatgtttt ctaattcttt gggcttatta ccgagaagca gcaggtggaa
    61441 caccgtttat aacaagaggt tgtgacagaa gtgggcaact tcaagaaata agaaggaatt
    61501 tacgaaaatt acttttcaag tttttgtgaa atttcggctt atttttcgaa gaagctactt
    61561 ctggaacacc gtttataaca agaggttgta acagaggttg tgacacctgt gtcttgctca
    61621 tggaggctta tctcgggcat cttattcctt taattttaca taaaaaggag gctgtgacgg
    61681 tttgtctcag cccctttcta ttgaaatctt gcattcaaat aacgttcttt atctatttat
    61741 ttcttgccgt actgggaact tggtctaccg taccaccaat tacctagtgt acgttgtagc
    61801 catggaatta cccagcgatc caacccaatc gcacgtccgg aaccgttcat caatgcgaaa
    61861 gcaacaaaaa tgaaccaaat gtttacccag taaaacatgc ctgataagca gaacgtgatc
    61921 accaatacaa tcgttgtcgc actgcatagc catgtgaata atccagcaat caatgctaat
    61981 gccaaggcaa tttcaaagaa taccatgaat ttttggaaga acattgctac atcattgttt
    62041 ggcatgatca cttgcatgat ctttgtgaac cagtctggtg acttctcaat cacagccatt
    62101 ggttcttcac cgtatgcata actcaaacca aaatgtgcag cttgagtcgc tgcttctccg
    62161 gcacttgccc ctgctccgct ggcagctgtg gttgcatcac cagcagcaga agcaccactg
    62221 gcaacatctg ttacattgta ttcccatgtc caagggaaca cactgtttcc agtgaaccat
    62281 gatccttcac caaaccaagt acttggtttg aacacttcac cattcccaat gatttttttg
    62341 gaagcttcaa cgaaccaaac taaaccatag aaaatacgaa gtggcacact ccataagaca
    62401 ttgccgtaac gagaagaatg tcctcgagtc acgttgcgat catctttgat atggaaaaac
    62461 tcatgcatta aatattggaa catataataa ccagaacgaa tatcaaagaa gtatttcaaa
    62521 ttcacgatat gtttcattac gatagctaaa aatccgctta aatggatctt atcaaatagg
    62581 ttagctaccc cccatttggc accaatagaa accataaatc cttgatagtt tcctttaaat
    62641 ttatgctttt ctccgccttt gatcgaagcc acgatattcg ctgcggctgt gtgtcctgtt
    62701 tgttcagcag cttgaacgat ctgcggtgtc ggtgttcctg ggaattcttc ataaaagact
    62761 aaatcaccaa tgatatagat gtttttatct tcgtagcctt ttgcctgcat atattcattg
    62821 gcgatcaacc gctgtccgcg tgctgattct aatccaaagt cagcagcgtc agaagttcct
    62881 ttgacaccag ctgtccaaat cagtgtgtga gtcggaatgt tgcgtccgtc tttcaatttg
    62941 atatgatcag cagccacttc tacgattggt gcattcaata ataattccac gttgtgctta
    63001 cgcaaatagc gttcagcttt tgcagcatca tttcgatcca acatattcaa gatcgtcggc
    63061 attgcctcta cgaccatcag tgtgatttcg tttggatcta atttataatc tttggctagg
    63121 cgatttttcc agtcgatcag ttccccaatc atttcaatac cggtaaatcc agaaccacag
    63181 acaacgaaag tcagcatggc tttacgcagt tcatcatctg gttccaatgc agcttttgct
    63241 actgtcttct caatatgttc ccgaatcctt acagaatctt caaatgacca aagggtgaat
    63301 ccgtgtgctt tgacaccagg tgtaccaaag tcattcggct ctccgcccat tccaaggatc
    63361 agctgatcaa aaggatagga acctaatttg gtcttgacga tttttcgttc tttatcgata
    63421 ccgattacag tgtctgtgac aagttgtaca ttcttttgac gagcaaacaa atgctgcaga
    63481 tcgtattgga ttgcttctgg ttctacacgt ccgccagcta cttcatgcaa ctcggtcatc
    63541 atagtatggt acgaatgacg atcgatcaga gtaattgcta catcttcttt tttcagtttc
    63601 tttgccaagt attttgtagc tgaaacaccg gcatatcctg ctcctacaac gacaatattt
    63661 tgctttgcca ttgatcatcc gctccttata gactatctat ataatgaata tatgataatt
    63721 cttttagttt gatacttatt acacaatctc catatattat atagatgttc tgttcttttg
    63781 tctaactcct tttggatttt tttcaaattt taaaataaag cgttttttca cgaaatttgc
    63841 tgaaagaaaa cgtttgaaat attacatggg ttatttatca aaaatagttg acttataggc
    63901 taaaaagatt agaatgtata atgaatcaca aagaaaaatt gtggactaga aatggaatgg
    63961 tggggtatta aaatgaagaa aaaacgtttt gtaactggat ttgccgtatt agcattttca
    64021 gctctagtgt taggcgcttg cggcgcagat gacaacggaa gctcaaactc ttcttctgaa
    64081 tcatctacag cacaatcttc gactgcaaaa agcacaacag aatcatctgc aaaagttgta
    64141 gctggcggag atttgcaaga cggcacatat aaattagaag aaaaaaatta ttcaaatggt
    64201 taccgtgcag tctttgaaat ggttgtaaaa gacggcaaaa tcactgaatc aaaatatgat
    64261 aacgtcaatg aaaacggcga atcaaaaaca aaagatgcag aatacaacaa aaacatggaa
    64321 gcaaaatctg gcacaaaccc agaaaaatac attcctgagt tgaatgacca attcttaaaa
    64381 gcacaaagcg caagcggtgt ggaagtcgta actggagcta ctcactcatc tgaaagcttc
    64441 caaaactacg cacaacaatt gatccaagct gctcaagctg gtaacacaga tacaatcgaa
    64501 atcgacaatg gtgctgattt gaaagacggc acttacaagc ttgaagaaaa gaactactct
    64561 aacggctacc gtgtgcaatt cgaaatgaca gttgctggcg gcaaagtaac tgaatcaaac
    64621 tttgattata tcgacaaaga cggtaaatca aaacaagatg atacagaata taatgaaaat
    64681 atgaaagcaa aaagcggtac agaaccaaaa acttacatcc ctactctgaa cgacgaattc
    64741 gtcaaagcaa tgggcgaaga agatggctca cctgctaatg tagaagttgt tacaggtgct
    64801 acacacagct cacattcatt catcatgtac gcacaacaat tagtgaatgc agctgaaaaa
    64861 ggcgacactc aaacaatcga agtagataat atcgtaacag aaaaataatc acataatcat
    64921 actaataaaa gttggagggt gggacgatag ccagattagg cagtcgtctc acctttcaaa
    64981 atgaagagag gaaaaagatg cggaaaaaac tactattttc cttaatcaca ctgggcatct
    65041 cttttatggt gctaactggc tgcagcaact cagcaaataa aacaaccact gaaagttcag
    65101 cagctaccaa gatattgaaa gatccatatt ctgatgaaca gttcttgtta gggacttacg
    65161 ttcgtatccg tgtgtatgat gaaggcaaag aatcagcatt gaaacctgcc ttcgatcgag
    65221 taaaagaact tggtgataaa atcacgatca atcaaaaagg ttctgagatc gatgaagtga
    65281 atgagcaagc tgggatcaaa ccagtaaaag tttctgatga cgtctacacg ttagtcaagc
    65341 gggcctacga atatagccaa gattctcaag gcggattcga tatggcgatt ggtgcaatca
    65401 cacaattatg gcgaatcggt tttgatgacg ccagaaaacc ttcccaagaa gaaatcgatc
    65461 aggcattgaa attagtggat tatcataaga tcgaactaaa tgacaaagaa aaaactgttt
    65521 atctaaaaga aaaaggaatg atcatcgatt taggtgcaat cgccaaaggt tacattacgg
    65581 acgaagtagt gaaagtcttg aaaaaacaag gagtgaccac tgccatcgtt gacttgggcg
    65641 gtaatgtcta tgtcttaggc cacagcccaa gaggcgaaaa ccaagactgg acagtcggca
    65701 tccaagatcc gaacatggct cgcggatctg ttttaggttc tattaaagaa cggaacaaaa
    65761 cattagttac ctctggaatt tacgagcgtt atttagaagt agatggcaaa aaataccatc
    65821 atttgtttga tccaaaaacg ggttatccgt tcgacaatga tatcgcaagt gtcacgatca
    65881 taacagataa atcaattgat ggtgacgggt tgtctacagc agtcttttca atgggtgtga
    65941 aaaaaggact ggaatacgtc gaaagcgaat tgaataacgg gacggaagcg atcttcgtga
    66001 caaaagacga taaagtatat gtgaccgatc caatcaaaga caccttcaaa ttatcggagg
    66061 actcccatta cacgatgggt gaccgttctg aactaaaata aggagtacga accatgacac
    66121 taaaaatctt tttagaagta gttgaaatac gaacaaaggt ggctagtgta tttccttttg
    66181 cggtaggggt attattctct atcgcttttt ttcatgagat acattggcta aacacttttt
    66241 tattttttat tggaatgcta gtttttgatt tagcgactac cgctatcaat a
//
LOCUS       DARBDV010000005        60244 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000005 DARBDV010000000
VERSION     DARBDV010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.005"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..821)
                     /locus_tag="SCM56_000328"
     CDS             complement(<1..821)
                     /locus_tag="SCM56_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEG1109158.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPT"
     gene            992..1360
                     /locus_tag="SCM56_000329"
     CDS             992..1360
                     /locus_tag="SCM56_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEG1109159.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            1530..1961
                     /gene="mraZ"
                     /locus_tag="SCM56_000330"
     CDS             1530..1961
                     /gene="mraZ"
                     /locus_tag="SCM56_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEG1109160.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            1982..2941
                     /gene="rsmH"
                     /locus_tag="SCM56_000331"
     CDS             1982..2941
                     /gene="rsmH"
                     /locus_tag="SCM56_000331"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEG1109161.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            2946..3347
                     /gene="ftsL"
                     /locus_tag="SCM56_000332"
     CDS             2946..3347
                     /gene="ftsL"
                     /locus_tag="SCM56_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEG1109162.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            3348..5540
                     /locus_tag="SCM56_000333"
     CDS             3348..5540
                     /locus_tag="SCM56_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEG1109163.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            5566..6528
                     /locus_tag="SCM56_000334"
     CDS             5566..6528
                     /locus_tag="SCM56_000334"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEG1109164.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            6542..7903
                     /locus_tag="SCM56_000335"
     CDS             6542..7903
                     /locus_tag="SCM56_000335"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEG1109165.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            7929..9017
                     /gene="murG"
                     /locus_tag="SCM56_000336"
     CDS             7929..9017
                     /gene="murG"
                     /locus_tag="SCM56_000336"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEG1109166.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            9032..10252
                     /locus_tag="SCM56_000337"
     CDS             9032..10252
                     /locus_tag="SCM56_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEG1109167.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            10408..11733
                     /gene="ftsA"
                     /locus_tag="SCM56_000338"
     CDS             10408..11733
                     /gene="ftsA"
                     /locus_tag="SCM56_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEG1109168.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            11756..12997
                     /gene="ftsZ"
                     /locus_tag="SCM56_000339"
     CDS             11756..12997
                     /gene="ftsZ"
                     /locus_tag="SCM56_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEG1109169.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            13014..13691
                     /locus_tag="SCM56_000340"
     CDS             13014..13691
                     /locus_tag="SCM56_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEG1109170.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            13705..14304
                     /locus_tag="SCM56_000341"
     CDS             13705..14304
                     /locus_tag="SCM56_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEG1109171.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            14319..14606
                     /locus_tag="SCM56_000342"
     CDS             14319..14606
                     /locus_tag="SCM56_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEG1109172.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            14655..15437
                     /locus_tag="SCM56_000343"
     CDS             14655..15437
                     /locus_tag="SCM56_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEG1109173.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            15458..16165
                     /locus_tag="SCM56_000344"
     CDS             15458..16165
                     /locus_tag="SCM56_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEG1109174.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            16427..19225
                     /gene="ileS"
                     /locus_tag="SCM56_000345"
     CDS             16427..19225
                     /gene="ileS"
                     /locus_tag="SCM56_000345"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEG1109175.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(19274..20800)
                     /locus_tag="SCM56_000346"
     CDS             complement(19274..20800)
                     /locus_tag="SCM56_000346"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEG1109176.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(20815..21462)
                     /locus_tag="SCM56_000347"
     CDS             complement(20815..21462)
                     /locus_tag="SCM56_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEG1109177.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(21646..21975)
                     /locus_tag="SCM56_000348"
     CDS             complement(21646..21975)
                     /locus_tag="SCM56_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HEG1109178.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            22152..22880
                     /gene="sfsA"
                     /locus_tag="SCM56_000349"
     CDS             22152..22880
                     /gene="sfsA"
                     /locus_tag="SCM56_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HEG1109179.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            22896..23909
                     /locus_tag="SCM56_000350"
     CDS             22896..23909
                     /locus_tag="SCM56_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG1109180.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            23909..25186
                     /locus_tag="SCM56_000351"
     CDS             23909..25186
                     /locus_tag="SCM56_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEG1109181.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            25249..27951
                     /locus_tag="SCM56_000352"
     CDS             25249..27951
                     /locus_tag="SCM56_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG1109182.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            28103..28420
                     /locus_tag="SCM56_000353"
     CDS             28103..28420
                     /locus_tag="SCM56_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1109183.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            28450..28770
                     /locus_tag="SCM56_000354"
     CDS             28450..28770
                     /locus_tag="SCM56_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEG1109184.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            28878..30338
                     /locus_tag="SCM56_000355"
     CDS             28878..30338
                     /locus_tag="SCM56_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1109185.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            30406..30627
                     /locus_tag="SCM56_000356"
     CDS             30406..30627
                     /locus_tag="SCM56_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109186.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            30658..30840
                     /locus_tag="SCM56_000357"
     CDS             30658..30840
                     /locus_tag="SCM56_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HEG1109187.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            30840..31253
                     /locus_tag="SCM56_000358"
     CDS             30840..31253
                     /locus_tag="SCM56_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HEG1109188.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            31376..32557
                     /locus_tag="SCM56_000359"
     CDS             31376..32557
                     /locus_tag="SCM56_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1109189.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(32628..32792)
                     /locus_tag="SCM56_000360"
     CDS             complement(32628..32792)
                     /locus_tag="SCM56_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109190.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            32946..33019
                     /locus_tag="SCM56_000361"
     tRNA            32946..33019
                     /locus_tag="SCM56_000361"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32980..32982,aa:Arg,seq:cct)
     gene            complement(33056..33256)
                     /locus_tag="SCM56_000362"
     CDS             complement(33056..33256)
                     /locus_tag="SCM56_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1109191.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(33561..34793)
                     /locus_tag="SCM56_000363"
     CDS             complement(33561..34793)
                     /locus_tag="SCM56_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEG1109192.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            35047..35616
                     /locus_tag="SCM56_000364"
     CDS             35047..35616
                     /locus_tag="SCM56_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1109193.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(35794..36234)
                     /locus_tag="SCM56_000365"
     CDS             complement(35794..36234)
                     /locus_tag="SCM56_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEG1109194.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            36392..37156
                     /gene="map"
                     /locus_tag="SCM56_000366"
     CDS             36392..37156
                     /gene="map"
                     /locus_tag="SCM56_000366"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEG1109195.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            37188..38111
                     /locus_tag="SCM56_000367"
     CDS             37188..38111
                     /locus_tag="SCM56_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEG1109196.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            38187..39326
                     /locus_tag="SCM56_000368"
     CDS             38187..39326
                     /locus_tag="SCM56_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEG1109197.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            39319..40119
                     /locus_tag="SCM56_000369"
     CDS             39319..40119
                     /locus_tag="SCM56_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEG1109198.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            40119..40946
                     /locus_tag="SCM56_000370"
     CDS             40119..40946
                     /locus_tag="SCM56_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEG1109199.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            40924..41658
                     /locus_tag="SCM56_000371"
     CDS             40924..41658
                     /locus_tag="SCM56_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEG1109200.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            41758..42624
                     /gene="rfbA"
                     /locus_tag="SCM56_000372"
     CDS             41758..42624
                     /gene="rfbA"
                     /locus_tag="SCM56_000372"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEG1109201.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            42638..43210
                     /gene="rfbC"
                     /locus_tag="SCM56_000373"
     CDS             42638..43210
                     /gene="rfbC"
                     /locus_tag="SCM56_000373"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEG1109202.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            43232..44260
                     /gene="rfbB"
                     /locus_tag="SCM56_000374"
     CDS             43232..44260
                     /gene="rfbB"
                     /locus_tag="SCM56_000374"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEG1109203.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            44358..45209
                     /gene="rfbD"
                     /locus_tag="SCM56_000375"
     CDS             44358..45209
                     /gene="rfbD"
                     /locus_tag="SCM56_000375"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEG1109204.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            45244..47277
                     /locus_tag="SCM56_000376"
     CDS             45244..47277
                     /locus_tag="SCM56_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109205.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            47321..48601
                     /locus_tag="SCM56_000377"
     CDS             47321..48601
                     /locus_tag="SCM56_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HEG1109206.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            48811..49617
                     /locus_tag="SCM56_000378"
     CDS             48811..49617
                     /locus_tag="SCM56_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1109207.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            49629..50849
                     /locus_tag="SCM56_000379"
     CDS             49629..50849
                     /locus_tag="SCM56_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1109208.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            50839..52425
                     /locus_tag="SCM56_000380"
     CDS             50839..52425
                     /locus_tag="SCM56_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEG1109209.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            52464..54602
                     /locus_tag="SCM56_000381"
     CDS             52464..54602
                     /locus_tag="SCM56_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEG1109210.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(54970..55953)
                     /locus_tag="SCM56_000382"
     CDS             complement(54970..55953)
                     /locus_tag="SCM56_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEG1109211.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            56331..57722
                     /locus_tag="SCM56_000383"
     CDS             56331..57722
                     /locus_tag="SCM56_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEG1109212.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            57738..58778
                     /locus_tag="SCM56_000384"
     CDS             57738..58778
                     /locus_tag="SCM56_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEG1109213.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            58797..59882
                     /locus_tag="SCM56_000385"
     CDS             58797..59882
                     /locus_tag="SCM56_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEG1109214.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            59915..>60244
                     /locus_tag="SCM56_000386"
     CDS             59915..>60244
                     /locus_tag="SCM56_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEG1109215.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYF"
BASE COUNT        19758 a        10156 c        12537 g        17793 t
ORIGIN      
        1 attgtcggta tagttaaaat aatcgttaaa gaagttaaaa ttttcataat attattcaga
       61 ttattcgata cgattgctga aaaagtctga ttcatttgtt ctactagttt taattgaatg
      121 cgagaagtag tcattgcctg ctctgtttcc accaatacat cacgtaaatg atttttcaaa
      181 gcatgatttt cttggaaaag tttattttgc gctagttctt tcaatgcatc taaattagca
      241 gaagtcgctt ctctgaaata aacgaggctt ttttggatat ccatgatttg ataaagttgt
      301 ttgttttcag tggatacttg aagtttggct tccaaagtat cgcattgaga ttgaagcgta
      361 gctaaataac tgttgtaaga gagtgtgacc tgccaaatga attgaaagaa gatattgaga
      421 ttaggcgaaa tatcactttt tggaaaggtc tggctaaaga ctttctttaa aaacagtggt
      481 tcatgattcg taattgtgat aagcttcttt ttgggcgtca aaataataga gagcgggtat
      541 gtatccactt gaaaataacc gctaggactt gttttagcaa aaggatattg taacaataat
      601 aacgctggct ttgtcaactt tgattgattg aattcttctg tacgggaatt ttctgcatcg
      661 tccaatatgg aagtgatgta atctgacgga atttcaaatt gatgtgtcag ctgatgaatc
      721 tcttcttgat taggacgttc tacacaatac cagatcgttt ggtcagtttc ctttccggta
      781 ttacgcagtt tctcattatc taatgtaaaa tatttgatca tgattttcct cctgttgcct
      841 gtttctttta ttataacgga tagggaattg gaatacatgg aatgtgtttg taagatatag
      901 aatataactg cataaattta aataaaatga aacagttgtg taaagtatat acagtatact
      961 tgaagcaacg ataaaaaagg agtttacgtt catgggcaca ttcacaccac ttatcttgtt
     1021 ttggtatatt tacccggtaa tcgttttgtt tggttgccaa tttcttgtaa aattattttc
     1081 gttaaatcgt cgttttaaaa taaaagcccc tgatttggcg attccttttt tgtggggcgg
     1141 tatgcatgca ttgtctagaa atacagggac aatttctatc ttaccgtttt tctttatttc
     1201 tgttttgcta atgggaattt gtattgctgt tttccaagca tattattatg aagaaattat
     1261 ttatccgcgt tatttcaaga tgacttggcg tttggttttt ctgttgacaa tcgtcttgta
     1321 cgctgtcttg atcattgtga acatactttc ttacctatga aaaaaataga aaagctgtaa
     1381 aaagtctttt aaaaggcttt ttgcagcttg atttttaaaa aaatgtttat gaataaaaaa
     1441 agttgttccc ggatttccca gaaaaggtgg tagaaagtgg gggattgtgg tagactaatt
     1501 ttagttagtg gaggaatggg gggctttcaa tgttgatggg tgaattccaa cataatattg
     1561 atgcaaaagg acgtttaatc gtgccctcaa aacttcgtga agaacttggc gaaaagtttg
     1621 ttctgacacg cggattggat ggctgtcttt tcggctatcc gatgagcgaa tgggaaaatc
     1681 tggaagctaa actgaacgaa atgccgttgg ctaaaaaaga cgcacgtact tttgtgcgat
     1741 tcttctattc ggctgccact gaatgcgaac ttgataaaca aggacgaatc aatataccca
     1801 gcacgttaag aaattatgca gctttgacaa aaggttgcgt agtcatcggt gtatctaacc
     1861 gaattgaaat ttgggatgag acacgatggc aagatttttc tgcagcagca gaagagaact
     1921 ttgacgaaat tgctgaaagt atgattgatt tcggactata agaataggaa gaggctgact
     1981 aatgactgaa acatttcaac actatacagt catgttgaaa gaaacggtag atggattgaa
     2041 catcaaaccg gatggcgttt atgtagactg tacactcggc ggtgccggac atagtgaata
     2101 tttattaagc caactaagtt ctaaagggca tctgtatgcg tttgaccaag atcaaaaagc
     2161 aatcgatcac gccaagctgc gtttagcttc ttatgtcgaa caaggtatgg tcacatttat
     2221 caaagcgaac ttcagagaat tagaagaacg cttgagtgag catgtagata aggtagacgg
     2281 cattttgtat gatctagggg tttcctctcc tcagctagat gaagcagaac gtggatttag
     2341 ttatcatcaa gatgcaccgt tggatatgcg catggatcag tcagctcctt tatccgctta
     2401 tcacgtaatt aatgaatatt cctaccatga attagtaaag attttcttcc gctatggcga
     2461 ggagaaattt tccaaacaaa ttgctcgtga aatcgaacga gtacgaaaaa caaaaccaat
     2521 ccaaacaact ggtgagcttg tcgaaatcat taaacaagtc atcccagctc ccgcaagaag
     2581 aaaaggcggg catcctgcaa aaagaatttt tcaagctgtg cggattgcag taaatgacga
     2641 actcggtgcc gaagaggcct ctttagaaca ggcaatccgg ttattgaaag tcaatggaag
     2701 aatcagcgta atcacattcc attcattgga agacagaatt gtcaaaagta tgtttaaaga
     2761 atatagtacg gtacaagatc taccaccggg acttcctgtc atacctgacg aattccaacc
     2821 agaactgaaa gtcattactc gtaaaccaat ccttccttca gaggaagagc taacagaaaa
     2881 caatcgatca cgcagtgcaa agttacgtat tgcagaaaaa ataagaatga aagaggagta
     2941 gaatgatggc agaattaaag aaaatgcaag attacgaata tgatttacct gaattggtgg
     3001 atcagccgga acaacaaatg cctgattctt cttcgaatca agaaatcatt cttccacaat
     3061 caccggcaaa acgattaaag cagatttctg ctttggaaaa aattgctgtg gcaagcgtgg
     3121 cgattgctat tgttgcttta tgtgttttaa cagttatgct gcgcacaaat atcagcggag
     3181 ctgaaaaaga aatcacgtca atacaaacag aaatcaatga taaaaagcaa gaaaaaacga
     3241 gtttagaaca agaaaaaaac gaactttctc gtactgaacg gattaaacaa atcgcagaag
     3301 aaaaaggatt gtctattaat gacgacaatt taaggaaagt gaagtaaatg agtcttaaaa
     3361 ataaaattcg gcggtttatg gaaaagaaaa atctaaatcc gatgaacaat cgcaaaaaag
     3421 taggtatcat tttatttgct acgagtattg gattgttctt tttatttgcc gtccgtttct
     3481 cgtatatcgt tattggaggt catgttgctg gtacctcatt agctgaaaaa acaaaacagt
     3541 tgtaccaagg aagtgaagta gtaaaagcaa aaagaggaac gatttatgat cgtaacggtg
     3601 tggcgctcgc agaagatgcc tcctcttatt caattaaagc gatactctca aaaacatata
     3661 cctctgggga taaaaaactg tatgttgaag aaaagaattt tgataaaata gcagaaatcc
     3721 tgcataagaa tctttctata gacaaaaaag atgcactgaa tattttagag gacggcgcaa
     3781 aaaaagagct gtatcaagtt gaatttggta gctatgggaa aaatatctcc caagaaacca
     3841 agcaaaacat cgaagcggac atgaaaaaag aaggtgtcgc tggtctttat tttgtcgacc
     3901 atcaggctcg gatgtatcca aatggcgtct tttcttctca ttttattgga tacgcagttc
     3961 cagacaaaga tgagaatggc ttagttggta aactaggttt ggagtctgct tacaatgata
     4021 tcttgagcgg taaagatggt aagattattt atcaaaaaga taattttcaa aatcctctgc
     4081 caggtacagt ggcagaagaa gaaaaagctg tcgacggaca agatatctac acgactttag
     4141 acagcagact acaaagttat ttagaaacac tgatggatca agtaaatgaa gaatatcagc
     4201 cagaagaatt gacagcagtc ctgatgaaag caaaaaccgg agaaatcttg gcaatgggac
     4261 aacgacctac tttcaatcct gaaacaatgg aaggattgac tggaaaagat gctatctggc
     4321 gtaatttctt ggtacaagat agttatgaac caggatcaac catgaaagtt ttcactactg
     4381 cagctgctat cgaagaagga gaattcaacg agaatgaaac cttccaatct ggaaaaatcc
     4441 aagtagaaga tgctacgatc aatgaccatg actttggtga aaaaggtgtg ctgaccatgc
     4501 gtcaagctct ttcatggtca agtaacgtcg ggatggtcat actggaacag cgattaggcg
     4561 gacgatggta taactattta caaaaactgg gatttggaca aagtactcat tctggattag
     4621 atgatgaagt aaacggtgct ttacctacat taaatattgt cgaccgagca atgagtgctt
     4681 atggacaagc ggtaggtgtg acgaatttcc aaatgatgaa agctttcact tctattgcga
     4741 ataacggtac aatgattcaa cctcgatata tcagcaaagt ggtggatccc caaactggag
     4801 aagagcgaac aactcaaaca gaagttctag ggcaaccttt ttcaaaagaa acaacagaaa
     4861 aagtccgtga atatatgaga gatgttgtcg aaagcgaaaa ctacggtagt gcttatggtg
     4921 tatatagtgt acctggatac aatgtatcgg caaaaaacgg aacagcccaa atcgcttctg
     4981 atacaggggg atatcaaaca ggagatacag cctacctgta ttcgattgtg gagatggttc
     5041 cttctgaaga tccagactat gtcttgtatc taacaatgaa acaccctaag acatatgatc
     5101 gaatggcatt ggctaaaatc gcaaatccat tgatgaaacg ggcaatggat tttaaagaaa
     5161 ctgaagaaga ttcagataca gaaacaaaaa cagaaaaagt atccgtagcc gattatcgta
     5221 atcttgaagc ggacgttgca gcagctgatg ctcaaaaaag tggactgcaa cctgtagtga
     5281 tcggtaatgg taaaaaagta caaaaacaat ctactgccaa cggtgatcag ttgatctctg
     5341 gagaaaaact gatcctatat actggtggtg ataagctgat gcctgacgta acaggatggt
     5401 caaaagcaga tatcatgaag ctggggaaaa tcctcggtat tgaagttagt tttgatggag
     5461 atggttattg tgtcaaacaa gaacttgctc catatgaaaa aatcactgaa gacaaactta
     5521 gttttacttt agaagaataa gaagagaaat aggagagaaa agagcatgga atggacacaa
     5581 gcattgattc ccattgtcag cagttgtgca atgacaattg ccgcaatgcc gctatttatt
     5641 ggttattttc aaatgaaaaa gcaaggtcag gcgattcgtg aggagggacc aaagtggcac
     5701 aatagcaaag ccggaactcc tacaatgggc ggcttagtgt ttctgatcgg ctctattttg
     5761 acaggaattt gggttggagc ttggcaaaaa cagctaactc ctactctttt catcttgctt
     5821 tttgtattag cgttatacgg agtaatcggc tttcttgatg attttataaa aatattcaag
     5881 aaaagaaaca tgggactgaa ctcgaagcag aaattgattg gacagattct agggggaatc
     5941 atcttttatc ttgtttaccg ttctgaaggt tatccaggtg tattgaattt cttcggacta
     6001 gatctacctt taggaatcat ttatggtatt ttcgctctct tctggcttgt tggattttca
     6061 aatgcagtaa atctgacaga tggcatcgat ggattagttg ctggcttagg aagtatctct
     6121 tttgcagctt acggtctaat tgcttggcat cagcatcagt atgatgtatt aatcatttgc
     6181 ttaagtgttt taggaggttt actgggtttc ttcgtctaca acagaaaacc tgccaagatt
     6241 tttatgggag atgtcggttc attagcgttg ggcggacttt tagcggcaat ttctataatg
     6301 ctaaaccaag aatggacatt gcttttgatt ggtttgatct atgttatgga aacagcaagt
     6361 gtgatgcttc aagttacttc ctttaagctt acaggaaaac gaatttttaa aatgtctcca
     6421 attcatcatc actttgaaat gtgcggatgg tcggagtgga agattgatac agttttttgg
     6481 ttgatcagca ttgtgacttc gttgatcaca ttatggatcg tttggtagga gatgacggga
     6541 aatgaaaaaa atatcaacat atgaaaataa gaaagtgctt gtcttaggac tcgcaaaaag
     6601 cggagtaagt gcggccaaat tattacatga attgggcgca cttgtcaccg ttaatgatgg
     6661 aaaacctttt gacgaaaatc cggaagcaca agagctgctt tctttaggga taaaagtaat
     6721 aacaggtagt catccaatcg aattgttgga tgaagaattt tcattgatgg tcaaaaatcc
     6781 agggattccc tacagtcatc ctttagtagc aaaagcccaa gaaatgggta ttccggtaat
     6841 tacggaagtt gagctagctt acgaagtagc ggaatgccca attattggga ttacaggaac
     6901 gaacggaaaa acaacgacaa caacgatgac aggactacta ctgaatgcag gagcagatca
     6961 aggaattgca cgtttagcgg gtaatattgg gtatccagca agtggtgtag cgcaagaagc
     7021 aaaatcagaa gataaaattg tgatggaatt gtctagcttc caactcatgg gaatcacaga
     7081 tttccgccct catatagcgg tgattaccaa tatctatgaa gcacatattg attatcatgg
     7141 tacaagaaaa gaatatgtca aagctaaatg gaaccttcaa aaaaacatga cagaaaaaga
     7201 ttatctgatc cttaactgga atcagtcaga actacaagaa ttagcgcaaa gaacaaaagc
     7261 acgcgtactg cctttctcga caaaagaagt gttggaagac ggtgtgtatg ctgacgatta
     7321 ctcaatatac tataaaaaag aaaaaataat ggaaatatct gaacttggcg taccaggtaa
     7381 gcataatgta gaaaatgcac tagctgctat ttctgtagca aaattgtatg gtatatcaaa
     7441 tgaagctata cgagaaacgt tgcatttttt ccatggcgta ccacatcgta cgcaatatgt
     7501 aggagaaatc caaggcagaa aattttataa tgattcaaaa gcaaccaata ttttagcgac
     7561 taaaatggct ctaagtggat ttgaaacgtc taaggtcgtg ttattggctg gcggattaga
     7621 cagaggaaat acatttgatg aattaattcc ttcattaaaa ggaatcaaag caatggtcgt
     7681 atttggccaa acaaaagaaa agttgatgga tgctggaaaa aaagcaggca tcgagacaat
     7741 agtaacagcc gattctgtag aacaagcagt tcctttagca ttagaaaaca gtacagatgg
     7801 tgatgtagtt ttattatcac cagcgaatgc cagctgggat caatacccaa attttgaaac
     7861 tagaggaaac agattcatgg aagctgtgaa ccgattaaaa tagaaaaagt agaagcgagc
     7921 gataaaaaat gaaaattttg gttacaggcg gaggaacagg gggacatatt tatccagctc
     7981 ttgcctttgt caattatgta aagactcaag aaccgaatgc tgaattcatg tatgtgggag
     8041 caaaacgagg tctagaaaac aaaatcgtcc ctgatacagg aatgcctttc cataccttgg
     8101 agattcaagg atttaaacga aaaatttcca tgcataacat caagacggtc cgattgtttc
     8161 ttaagagtat ccgtcaagca aaaaaaatcc tcagtgattt tcaaccagat atcgttatcg
     8221 gtactggagg atatgtttcc ggagcggtag tgtacgctgc agccaaaatg ggtattccaa
     8281 cagttatcca tgaacaaaac agtgtaccag gtgttaccaa taaattttta agccgatatg
     8341 tggatcggat cgctctttca tttaaagatg cggcttcctt ttttcctgaa aacaaagcag
     8401 ttcttgtagg taatcctcga gcacaagaag tagcagatac taaaaaatca gatattttga
     8461 aaacatttga tttggatcca gaaaagaaaa cagtcttgat ttttggcggt agtcaaggag
     8521 cacttaaaat caatcaagca gtatcagttt ttttaagtga atatattcct tcttcttacc
     8581 aagttttgta tgcttcagga gatagatatt atgaagagat cctaaataaa ataaatcctg
     8641 aaatacaaaa tattagtgtc cagccttata tcaaaaatat ggcagaggtc atggcttcca
     8701 gtgatctgct ggtggggcgt gctggtgcaa cttcgatcgc agaattcaca gctctggggt
     8761 taccggcagt gttggttccc agcccatatg ttacgaatga tcatcagact aaaaatgcaa
     8821 tgagtcttgt tcacgcagga gcagcaaaaa tgattgcaga taatgaacta acaggtgaat
     8881 cactaagtca gacagtcaac gaaatcatgg gtgatgaaga attgcaaaag caaatgtgcc
     8941 gagcttctaa agaacaagga atccctgatg cttcaaaacg tctttatgac ttagtaaaac
     9001 aaataatcaa aaaataaagg cggtggtctt agtgagtaag aaaaaagaac caaaaaaatt
     9061 gtctcatccg tcttctgatg aaaacaattt gactccttgg caaaaggcta accgaaaata
     9121 tctggcagaa caagagatga aaaaccaaga gccaaaaaaa gaagacataa cattagatac
     9181 acccctttca gattctgaag aagaaaaaac ggttcaagag gaacagcaag aagaaagcca
     9241 gtccgagaaa aaaatcgaat tttttgaaga gatcaagcaa aagaaaaacg gtggaccata
     9301 taacggttct tttttgaacc ggctgcccaa tttaaaaagt caacggcaca aagtactatt
     9361 tcgtcggtta gctttgatca tcactgtatt gggtataccg ctgatttttt tgatctatta
     9421 tgtttctcct tatagcaaat tacaagcagt tgcagtatca ggcaataaga cagtcaacag
     9481 ccaagaaatc atctctgata caaaattgtc attaggagaa aatgtttggg gacagtattt
     9541 tcatcgatct acttatatcg atcgccttaa aaaagcacag ccaagaatcg aaacagcaaa
     9601 tatacatttc aaagggatga atgaattcga tttggatgta acagaatata aagagattgc
     9661 tttgatcgca aaaaacaatc aatactaccc agtgattgaa aatggaacag tattagatga
     9721 aaaagtagca aatcctacaa aaaatctccc tatcttggaa gagttcaaag actctgcaaa
     9781 aatcaaagaa ttaacaaagc aatacaatca gttatcatca gaattacaaa aagcaatttc
     9841 tgaaatcaag tacacgccaa gagcaagcaa taaaaatctg atccaattga atatgaatga
     9901 cggcaatcaa gttattgtaa atatcaataa tcttgcgaac caaatgaaat attattcgca
     9961 agtagccaaa gatatggatg aaaaaggtgt gatcgacatg gaagttggta tcttctcgca
    10021 tccttacggt agcactcaag aaacggaaga atcgggccaa tcggaagaaa caactgaaag
    10081 tgaagaaaat gcggatcaaa caaacgaatc aagtgaagag gcagaaacgc aaaaagcaca
    10141 aacacctgga tcgtctagca caaacggatc atctgattca aatgattcaa gtgaaacaag
    10201 tcaaatagaa gataatccaa gtacaactcc gtcaagttta aatgaaaatt gataaaacaa
    10261 ctttaaaaag taagaatgta ctttctaaaa accagtaatt tatggtagaa ttggaagcag
    10321 tgaatatatc tattaataag tatattttaa aacggacgct agttgttcgc ttgtatatgg
    10381 aatagtaagt aggagggaat cccattcatg gcaaaaacag gaatgtatgt aggccttgat
    10441 attggaacga catctgttaa agttgtcgtg gctgaatata tcgacagcca aatgaatatt
    10501 attggagtag gaaacgcaaa atcagaaggg attaaccgag gcattatcgt tgatatcgat
    10561 aaaacggtcc aagcgataca acgagcagta cgacaagcag aagaaaaagc aggtatccag
    10621 atcaaaggcg taagtgtcgg tttgccagca aacatgcttg aagtagaaaa ttgccaggga
    10681 atgattgcag ttaacggaga ttcaaaggaa atcaccgatg aagatgtcag aaatgtggct
    10741 tcagctgccc ttgttcgttc aatacctcct gaacgtcaaa tcgtgtcaat attgccacaa
    10801 gattttactg tcgatgggtt cgagggaatc aaagatcctc gtggaatgat cggcgtaaga
    10861 ttagaaatgt acggtctgtt gtttacagga cctaaaacga tcatccataa catacgtaaa
    10921 tgcgtggaaa atgccggatt aatcgtcaac gaaatggtga ttactccttt agcgctgact
    10981 gaatcaattc tttctgacgg agaaaaagat tttggtacga tcgtcattga tatgggtggc
    11041 ggccaaacaa caacggctgt tatgcatgat aagcaattga agtttaccaa tcttgaccaa
    11101 gaaggtggcg aatttgtcac taaagatatc tctattgtat taaatacgtc atttaacaat
    11161 gcagaagcgt tgaaaataaa ctacggggat gcatacccag aacggacatc accagatgaa
    11221 gaatttccag tagatgtgat tggtcagtca gaaccagtga aagtcgatga acgctacttg
    11281 tcagaaatta tttcagctcg aatggaacag attttcaaca aagcaaaaga tgcattggac
    11341 caaatcgaag cattagagtt gccaggtggc gtcgtattga caggcggagc tgctagtctt
    11401 cctggtgttg tggacttggc acaagaaatc ttcggcgtaa atgtaaaatt gtatgttcca
    11461 aatcacatgg ggctgagaaa cccggtattt acaaatgtaa taagtatcgt ggattattca
    11521 gcaaacttga gtgaagtgta tcaattggca aaaattgctg ttacgggtga aacctcagca
    11581 gctcgtcaaa tggtagttga acaagaaaca acaaacacat atgagagcta tgaagcacct
    11641 gaagaaacaa tctacgatga gccggaacca aaagagtccg gagaaaatgt aaaaaataaa
    11701 atcaagggtt tctttacaaa tatttttgat taattcatcc tgggaggaat aagatatgga
    11761 attctcaatt gataataata tcaatgacgg cgcagtaatc aaagtcatcg gagtcggtgg
    11821 tggcggtgga aatgctgtaa accgaatgat tgaagaaaac gttaaaggtg ttgaattcat
    11881 tacagccaac acagacgtgc aagcattaaa aaattcaaaa gctgaaacag ttatccaatt
    11941 aggccccaaa tatactcgtg gtttaggtgc tggttctcag ccagaagttg gacaaaaagc
    12001 ggcagaagaa agtgaacaat ccttacgcga agctttagac ggtgcagata tgattttcat
    12061 cactgcagga atgggtggag gaaccggtac aggtgctgca ccaatcgttg caggtatcgc
    12121 gaaagaattg ggcgcattga ctgttggtgt cgtgacacgt ccattcactt ttgaaggacc
    12181 gaaacgtggc cgttttgctg cagaaggtat cgctcgtttg aaagaaaatg tggatacatt
    12241 attaattatc tcaaataacc gtttattaga agttgtagat aaaaagacac cgatgcttga
    12301 agctttccgt gaagcagata atgttttacg acaaggtgta caaggtatct ctgatttgat
    12361 cacagcgcca ggttacgtta accttgactt tgctgacgtg aaaacagtga tggaaaacca
    12421 aggtacagca ttgatgggga tcggtgtggc tagtggagaa gatcgcgtag tagaagcaac
    12481 gaaaaaagca atctcctctc cattgcttga aacatctatt gatggagctg aacaagtact
    12541 tcttaacatc acaggcggtt tggatatgac attgttcgaa gcacaagatg cttcagatat
    12601 cgtagcaaat gcagcaactg gcgatgtaaa tatcattcta ggtacatcta tcaatgaaga
    12661 aatgggcgat gaaatccgtg tgacggtcat cgcaacaggt atcgatgaat caaaaaagga
    12721 acgcaagtct agccgaccag ctagacaagc acaaatgcaa tcacctgctc aaaagactgt
    12781 attagatatg gatcaagcta aaccaacttc ttcagaagaa gaaaacagtt ttggcgattg
    12841 ggatatcaga cgtgaacaaa atgttcgtcc tagagttgat gattccaatt ttgacaatat
    12901 cgaaaaaaaa gaatttgata cattcaaccg cgaagaaaca aaatctcatg gcgatgatga
    12961 attaaatacg ccaccgtttt tccgtcgtaa aaaataaagg agaggaagag cacatgattg
    13021 ctgacaactt gcagaaaatc aagcaagaaa ttcaggattc gtgtgctctt gttcatcgtg
    13081 atccgaaaga agttacatta gtagctgtaa caaaatctgt tgaatccgct caagctgaag
    13141 aattggttag agctggcgtt atgaattgtg cagaaaaccg agtagataaa ttattagaaa
    13201 agaaacaaga tttatttgat tttcctgaaa taaagtggca tttaattggt aatttacagc
    13261 gcagaaaggt aaaattgatt gtaaatgaaa ttgattactt tcatgcgttg gacagtttaa
    13321 gattagcaga agaaatccag aagcggttag agaaagaatt atcctgtttt attgaagtaa
    13381 atgtttctgg tgaggaatca aaacatggaa tttcaccaga tgagctttta ccgtttgtgg
    13441 aatctttagc tgcctgcaca aaaatcaagg ttgttgggtt gatgactatg gcgcctaaag
    13501 aagcttctga tgagcaaatc cataaagtgt ttggtacgct gaaagaattg cgtgatcttg
    13561 ttcaagcaaa aggttatgcc catgcaccat gtagtgaatt aagtatgggg atgagtcaag
    13621 actttcaaat tgcgattgaa gaaggtgcaa catttatccg tgtaggaaca gcgctattca
    13681 aagacgaata aaggaggaac aaaaatgccg cttttaaaca gaggagcaat tgctaatttt
    13741 ttcggattag ctgatgagga agagtacgaa taccaagatt atcctgaaac aacaaaaaag
    13801 gctgtacagc caactaaaaa aaatacaaca cagaatcaat caactcattc tgtatctgct
    13861 gcgaatactc atgaaaaaca aaaaacggcg acttcacgtc ctgtaacgca tgaaagtatt
    13921 cagccggctg aaaagaaaaa agtaattgaa ctacatcaat cacctacaaa aactgtagcg
    13981 aaagaaagaa cagcttcaag aacctctaca caacctaaag caaatcttgc tggaaaaatc
    14041 actgtaatgg aaccaagagc ttattctgaa gctatggcga ttgctaaacg aatcattgct
    14101 ggagaagcaa ccttagtgaa ttttcattta gtagaagaaa aacaagcgag aagaatcgtt
    14161 gattttctaa caggaacagt ttatgcacta gatggagata tcaaacgtgt tggagatgag
    14221 atttttcttt gtacaccgac tgggatggag attgacagtt caaccgctca atcttttgca
    14281 gatgcgaatt tgtttgatct gtaagaggag gaactacgat ttatactatt gcactattac
    14341 ttcatttact tcaacgggca gcctatttct atagcattat cttgatggta tatgcattgc
    14401 tctcttggtt tccaggcggg tatcaatcag ggctaggacg attattagct aagatctgcg
    14461 aaccgtatat cagtttgttc gatcgtctgc ctttgaggat tggacccttg gatttcacaa
    14521 ttttaggggc aatcctgatc ttgaacttag ccgtacaagg tttagcacag atcgttatat
    14581 ggataactgt ccatttagtt tattgattga agcaacaaaa tctttattct cagaatagat
    14641 aggaggggaa aattttggat gcaaatgtat accagcactt tcgggcagat gaacgtacat
    14701 ttatcgattc ggtaggcgat tggattgaac aggttcaagg acaatatgca ccctatctga
    14761 ccgaatttct tgacccaaga caatcctata ttttagaaac actgattcga caaagcagtg
    14821 atttgcgttt catgttttat ggcggatatg aacaggcaga aagaaaaaga tgtttgattt
    14881 tccccgatta ttacgaacct gtagaggaag attttgaaac agcattgtat gaaatccatt
    14941 atcctagcaa atttgccagc ctaagtcatg ggaaaatcct tggaacttta gtgggaacgg
    15001 ggatcaaacg ctcttacttt ggcgatatca tctcggacgg tgatcgctgg caagtattta
    15061 ttgcaaatga ggtggatcat tttgttgctg cacaagtaac aaagattgga aaggttgctg
    15121 tacgcttgga accaatcaaa tacacagaaa tgattatgcc gaaagatagc tggacacaag
    15181 aaagaggaac agttagttcc atgcgactag acagtgtgat ctctgagatt tataatattt
    15241 ccagacagcg gtccaagcag ctgatcgaag ctggtaaagt aaaagtgaac tggacagaaa
    15301 atacaagacc tgattttgta cttgaactat tagatattat ttcagtcaga ggttatggca
    15361 gagtccagat ccaagaactt gaggggaaga cgaaaaaaga aaagtacagg gtactgttcg
    15421 gtgtattacg taaataaaat aaaaaggtgg tttactcatg gctttaactc cattagatat
    15481 ccaaaataag acattcccga caaaaatgcg gggatataat caagacgaag tagatgattt
    15541 cttagattta gttgttcgtg actatgagga attgactcaa agaaatcgag aattagaaaa
    15601 agcagtcaaa cattctgaag aaaaacttga gtacttcaat gaattaaaag atgctttgaa
    15661 ccaatcaatc atcgtagctc aagatacggc tgataaagta aaaacaagtg caagcaagga
    15721 atccgaagtg atcgttactt ctgcacaaaa caaagcagat gagttagttg cgaacgctga
    15781 aaaacgtgca catcaattaa caactgatgc agaagaaaaa gcaagaaaaa tcttgacaga
    15841 tgcaacagaa aaagcaagac agcttgcgac agaaactgaa gatcttaaga aaaaaacacg
    15901 agtattccat caacgtatca gcttgatgct tgagtctcaa ttagaacaag tgaaaagtcc
    15961 ggaatgggat gaaattttac aaccattctc cagctacgtg actgacagcc atgaagtgat
    16021 caaagaagtt ttagcaaaac agcttgacaa tgaaaatgat actgatgtaa actcagacac
    16081 aggagtcata gaagcaccta tgacggcatt tgatacacag gctattgata tctcgatcgt
    16141 gcctggtgaa aatgaaaacg agtagaacag aaaagcactt tgttattctt aaagcgagcc
    16201 gatgagagtg gaagttcggt aagacactgc atggtgaaag atcctctact tgagacctgc
    16261 ttttccgaaa ttagagtaag aagcagcggc tgacctcgtt attggtcaca gagagtttgc
    16321 actagataga tgcaactaaa tttgggtggt accacggcat gttcgtccct tgcggctgaa
    16381 catgcttttt tgtgtccgga aaacaaataa aggagtgtat ggttgaatga aaatgaaaga
    16441 aaccttgcaa ttaggaaaaa caaaattccc tatgcgtggt aatttgccaa accgagaagg
    16501 cgagtggcaa aaagaatggg aagaaaatcg aatctaccaa aaaagacaag agttgaacga
    16561 aggaaaacct agctttgttc ttcatgatgg acctccatat gccaatggta atatccattt
    16621 aggacattca ttgaataaaa tcagtaaaga cattatcatt cgttcaaaat caatgtctgg
    16681 ctatcgtgca ccatatgttc caggatggga tacacatgga ttgccgattg aacaagtatt
    16741 ggctaataaa ggcgtgaaac gtaaagaaat gtccatggca gaatacttgg aaaaatgtcg
    16801 tgaatatgca ctttcccaag tagataagca acgagcagat ttcaaacgcc taggagttgc
    16861 aggagattgg gaacatcctt atgttacatt agatccttct tatgaagctg cacaagtacg
    16921 cgtatttggt aaaatggctg aaaaaggcta tatttacaaa ggattgaaac caatttactg
    16981 gtctccgtca agtgagtctt ctttagcgga agctgaaatt gaatataaag atgtaaaatc
    17041 tccatctatt tttgtagctt tcaatgtcaa agacggcaaa gatattcttg atgaagatac
    17101 agcttttgtc atttggacga caacaccatg gacattgcca gcaaaccaag gtattgctgt
    17161 aaatccaaaa tttacgtatg tattggtgga agccgatggc cgtaagtttg tcgtagcgaa
    17221 agatttgcta gaaacagtcc aatcagaaat cggttgggaa aatgtaacag ttctaaaaga
    17281 atttgctgga caagaaatgg aatatatgac tgcaagtcat ccattctatg aacgtgaatc
    17341 acttgtgatt ttaggcgatc atgttacttt agacgctggt actggattag tccatactgc
    17401 tccaggacat ggggaagatg actatatcgc tggtagcaaa tataaactat ctgtagtgtc
    17461 acctgtagac tcaaaaggat tgtttacaga agaagcacct ggatttgaag gtatctttta
    17521 tgacaaagca aatcctatga tcactgatct attaaaagaa aaaggagctt tgctgaagct
    17581 agatttcttt actcatagtt atcctcacga ttggcggacg aaaaagcctg tgatctatcg
    17641 ggcaactcca caatggtttg catctatcga taaattccgt cagaatattt tggatgaagt
    17701 agaaaaagta gagtgggtta tcccatgggg taaaacacgt ttgtataata tgatccgtga
    17761 ccgcggcgat tgggtaattt cccgtcagcg tgcatgggga gtaccacttc cgattttcta
    17821 tgctgaaaat ggagaagcaa tcatcacacc agaaacaatc gaacatgtgg caaaattatt
    17881 tgctgaacat ggatcaatta tctggtttga aaaagaagcg aaagaacttc ttcctgaagg
    17941 atttacccac ccaggttctc ctaatggcga atttacaaaa gaaaaagata tcatggatgt
    18001 ctggtttgat tctggttctt ctcatgaagc tgttttacgt cagcgaccag aattatcatt
    18061 cccagcagat atgtatttag aaggatcaga ccaatatcgt ggctggttca actcaagtat
    18121 cacgactagt gtggctatca atggtgtagc accatacaaa tcagttattt ctcaaggatt
    18181 tgctttagat ggtgaaggcc gtaagatgag taaatcttta ggaaacatca ttgcaccaga
    18241 aaaagtgatc aaacaatttg gtgcagatat ccttcgctta tgggtatcta gtgtagatta
    18301 tgaagcagat gttcgtgttt ctatggatat cttaagtcaa gtggcagaag tatatcgtaa
    18361 aatcagaaat acgatgcgtt tcttattagc gaatacagaa gatttcgatc ctaaaaaaga
    18421 tactgtttcg tacaatgatt tacgttctgt agacaaatat atgaccgttc gattgaacca
    18481 agtaatcaaa gaaatccgag aagaagggta cgacaaatat aacttcatgc atatttaccg
    18541 tacagttatg aacttcttga cggttgatct atcgtcattc tacctggatt ttgctaaaga
    18601 tgtagtttat attgaagcag aagatgatta tcaacgtcgt tgcatgcaaa ctgttttcta
    18661 tcaaacagct gtagctttga caaaattatt gactcctatc attccgcata cagcagaaga
    18721 aatctggtca ttcctgaaag aagaagagga gtacgttcag ttaagcgagt ttcctggtta
    18781 tgaagagttt gcaaaccaag aagaattgat ggatacctgg actgctttca tggacttccg
    18841 tgataatgtc ttaaaagcat tggaagaagc aagaaacagc aaattgattg gtaaatcttt
    18901 ggaagcaaaa ctaactgttt atccaaaaca acaagttcgt gaaatgttaa aagctttgga
    18961 tgcagatatt gcacaattat tgatcgtttc accagactac tttgaagtta tggcagcaga
    19021 tgaacaagtg ccagataacg ctatggtatt tgatgatgtt gctattacag tggaaaaagc
    19081 agacggggaa acttgcgatc gctgtcgcca agttcgtaaa gatgtaggag tagatgaaaa
    19141 acttcctcat ctatgcggtc gttgtgcaaa aatcgttgaa gacttctatc ctgaagctgt
    19201 agctgaagga ttcgaagaaa agtaaaaggg aaaagggggt gtgacatgtt ctttgtcaca
    19261 cccccttttg ttatcaatcg tttaattttc cacgttcacg ataccagtta tctggttgcc
    19321 agatccattc gaatccgtct ttttcaagta gttcgaaagc tgctttaggt cccattgatc
    19381 cagctttgta gttgggaaaa tccggttgtg tttggtccca agcttttcga atgacatcca
    19441 cgatatgcca agattgagcg acttcttccc aatgagagaa gttcgttcca tcaccattca
    19501 acgcatctaa tagtaatttt tcgtaagctt ctgggctatt ttcaaccatt tctgcactat
    19561 ttctaaattc caatttgatt ggttccgttt caaatccttg ccctacttct ttcccattca
    19621 aggataaaga aaatccttca gttggctgaa tatagatcgt tagaacatta ggtggaagtg
    19681 tcgtatcgtc acatggttca tcgacagatg ttttgaatac attaacagga acttgtttga
    19741 atacgatgtt gatccgagtc cctttttcag tcaaacgttt gcctgtacgt acatagaaag
    19801 gaacacctga ccaacggaag ttatcgatag tgaatttgcc tgcgacgaat gtttcagtct
    19861 ctgattgagc atccactttg tcttcttcgc gatagccctt gaattgtaca ccatccaatt
    19921 caccttgagc atattgcccg cgaacgaagt tttctaacac ttcttcttcg ttataaatgc
    19981 ggatagcttt caatgctttg atcttttctg tacggatttc tttttcggaa aatgctactg
    20041 gcggttccat tgcaagtaat gctacaacct gtaaaatatg attttgcacc atgtctttaa
    20101 gtgcgccact atgatcgtag tagcccccgc gatcttctac accgaggttt tcgccaaaag
    20161 taatttggat attgtcgata tagcgattgt tccattgagc ctcaaaaata ttattggcaa
    20221 aacggatagc tgagatgttt tggatcattt cttttcctaa ataatgatca atcctgaaaa
    20281 tatcttgttc tgggaatact tctctgattt gctggttcaa ttcaaaagca gactggtaat
    20341 cagaaccgaa cggtttttct atgatcaaac gattgaagcc attttttgtc ataatatttt
    20401 gtgatttcaa atgagaaacg atcgttccaa aaaattgagg tgccatagcc aaataataga
    20461 cacggttacc gtttaattga tagtgtccat ctaatcggtc agacaattct tttaatgtct
    20521 gataatgcgt tgaatcattt acatcatggg attgatagta aaaatggctt gcaaaggtag
    20581 ttgcttcttc atctgaagga ttcaatgcac ggattgtttc tttgacaact tcacggtagt
    20641 gctcatcgct ccagggacgg cgagcggttc cgattacagc aaaatgctcg cctaaatttc
    20701 cttttctgta aaggcggaac aaagacgggt acaatttacg ctgggctagg tcacctgtac
    20761 cgccgaatat cgtaaacaat acattttttt cattagacat gtggatacac tcctttatat
    20821 tttatcgaca aaaatcgtgc tggccgcctt aaagctgacg gggattttct ttctatcatt
    20881 ttggatagtg atcggtcctt cataagctgc gatgtcaacg atctcatatt cttcgttgat
    20941 attcaggtca atagtgacta agtaatccaa caattctttt tcatcaagca ctcgtgcgat
    21001 acgcactttt gtaccgacag gataggactc caatgtttgt cggcgttctt catggacaac
    21061 ttcctctttt ttaggaatcg atcctccatg aggacaatgt tcaggatgat tcaaatagtt
    21121 ttctaaatga tctgcaagat gttccgaagt cacatgttcc aatacttcag catcatcatg
    21181 gacttcattc cagctatagt ctaaatgttc aactaaaaaa acttcccata agcgatgttt
    21241 gcgaatcaga ctgctagctt tttgcagtcc gcttgcagtc aattgtacac cttgataagg
    21301 ggaatgttct acaagctctt ctttcacaag tttagaaatc atttcactga cagatgcagc
    21361 tgagacatct agactagata cgatttgttt attgttgact ttcgttgtat ctccacctag
    21421 ttctaaaatg attttcaaat aatcttctcg atttggtgtc atattcgatc atcctttcat
    21481 ggtaaaaaac catcattagt aagtgtaaca aaaaaaacgt taattgacta tttttctaaa
    21541 taaaaaacaa aggattttta gacgttttta caacaaaaaa cgaaagatga tagagaagac
    21601 tcattctttc gtttataaag gatatatata tacattggga gggatctatt tttcagataa
    21661 caacttatat gaagtgtctg cagattcgtc ataaagagtt tgttcgggat aagaaaagat
    21721 aagataaaaa ccatactgat cacgtacgac taaacgatat tcatctattt ctaccacaga
    21781 tgtgccaaga gactgaccgt tgatcaaaaa cgtgtaatca gatgtaatct gtaattgttg
    21841 tttttgctgg ctatcactaa agaaccatgt accttgatac tttgagagtc cttctgactt
    21901 ttccgcttgt tttttctttt tcaagtaaat aaaacttgag agaacggtaa tgagtgaacc
    21961 aagaaaatag atcatttttt ttatcaccaa atcactcctt tatgagaaaa taatcgcata
    22021 gacatattct ttcttcttta tttcattgta ccgaataaaa gctaaaattt ctctaagact
    22081 ttcgactggt ttttttactg tttttctaca aattatgata ggatacatag taggaagaaa
    22141 ggtgacataa tttgaagtat cagcaagtgg aaatagcttt ttttattgac cgcccgaatc
    22201 ggtttatagc acattgtatg aaaaaagacg gagaaataat tattgcgcat gtcaaaaata
    22261 caggtagagg aaaagaggtc ctgataccag gcgctgaagt ggctattgta tttgctcctg
    22321 gacctaaacg gaaaaccgcc tatgatctga ttgctgtaaa aaagaagggt gactggttta
    22381 atatcgatag tcaattgcct aatcggctgg caattgatgg tattttagac ggcacgatcc
    22441 ttttaccagg attaactggt ccatttacgg tatacaagcg tgaagtaact tatcttcact
    22501 ccaaatttga tatttattta gagacggtgg tcggacaaaa agcatttgtt gaagtcaaag
    22561 gtatgacact tgaaaataaa gcgattggtg cttttcctga tgcgcccaca ctcagaggat
    22621 tgaaacatat aggagaatta acgtatgccg cacaagatgg ttatgccgct tatgtcttgt
    22681 ttattgctca gttcgaacat cttcatctgg caacgatcca tgaagaaatg caaccagcac
    22741 tagctgatat ggttcgacat gcgcagcaaa gcggagttca aatcctcgcc tataattgtc
    22801 aagtatcggc agatgaggta actgtcaagc aggccattcc atttgatgta gatatccctt
    22861 ttgaagatcc tatacaatag aaagaaggta aaataatgat caacttaggt attatcggaa
    22921 caaattggat tactcatcaa tttgtccaag cagctttaag cacaaaaaaa tatgaattga
    22981 cagcggtata ttctagacat ttggaaaaag cacaggaatt tggttctcat tatgaagggg
    23041 atatcgaatt cgcaacagat ttgaagactt ttttcaatat tactcatatg gacaccattt
    23101 atattgcttc tccgaatagt cttcacttta gtcaagctaa gcaagcaatt ttggcaggaa
    23161 aaaatgtgat agttgaaaag ccagcttttt cgacgccaaa agaaatggat gaaatcatcc
    23221 atttggcaaa tgaacacaat gtgtattttt ttgaagcagc aagaaatatc catgaatcag
    23281 gttttaaaaa gattgccgat tacttaccat taaaagacga gataatcggt gcaaatttca
    23341 gctatatgaa atattcttcg cgctatgacc aagtattaga tggagaagaa ccgaatatct
    23401 tctcgactca tttctcaggt ggtgcattga tggatttagg tgtttacctt gtatatgcag
    23461 ctgttgcttg gtttggaatg ccaaaagaag tccattattt cccaagaaag atttctactg
    23521 gtgtagatgg tcttggatgg gggatcttac gttatgagaa ttttgatgta tcgatccagc
    23581 caggtaaaat cggtgattct tatttaccgt cagagattta ttttgactca ggaacgctag
    23641 tgatgaatgg agtcaatgca attgaacaag cacaatttca tgatcgcgcg cataaagaaa
    23701 tcattgattt ggcggtaaca gctgcagaaa atccaatggc agatgaagca gaggattttg
    23761 ccaatgtcat ggctcatccg aaagatccag cttggggatt gttgtatgaa gagtggatcg
    23821 agctagctag aaatgtgaac caagtgattt atgatttaag aaaaaatggc ggcatcaaat
    23881 tcgatgcaga taatgaggaa gatttttaat gaacgaattt gtacaagtat ttcccgaaaa
    23941 ttggcagaaa aaatatgagg ataaagggtt tatctcacct tctcttattc aacgagcagt
    24001 ttttcagcca ctaagtaata aacaaagtat agtagcggtt tctcctacag gttcaggtaa
    24061 aacactggct tatctttggc ctctactact aaacgtggaa ccaggtgaag caagtgcgtt
    24121 ggtcattttt gcttcttctc aagaactagc tattcaagta gcagatgttg cacgtgaatg
    24181 gggaaaagac aaagagttga aagtccaatc tttggtgggc ggagccaacg tcaaacgtca
    24241 gattgagggt ttgaaggaaa aaccagaaat cttgattggt acaccaggaa gaatattgga
    24301 attgatgaaa gcaaaaaaaa taaaagcaca tcaagtgaaa acaatggtat ttgatgaagc
    24361 agaccagcta tttgacgctg ggaacagcca aatcattgat caaattcttc atcaagctcc
    24421 aacagagtat cagctagctt ttttttcagc gacagcagat cgttcccttg aaagcattga
    24481 aaaaatcact gggaaaacgt ttgagacaat tgacgtgaca gcagaggacg attctcgaaa
    24541 aggactgaga cattactttt tacgtgtacc gatacgaaag aaagatgact acttgagacg
    24601 tctcacgcat atagaagatt ttcatggtct gattttcttt aatcaactga ctgaattagg
    24661 cgtaatggaa gaaaaattgc tgtatagagg tgtaccggta ggaagtctgg cctctgatca
    24721 gaacaagcta ctccgaaaag cagcgctaga acaattcaaa aagaaaatgg tcagagcact
    24781 tttgaccaca gatgtggcgg ctagaggttt agatattaca ggtcttcctt atgtagtgaa
    24841 tgcagaagtt ccgttaagtg aagaagctta tttgcatcgt tcgggaagaa ctggtcgaat
    24901 gggtaatgaa ggaatagtaa ttacactcgt acaagataat aatctaagag atttaaaacg
    24961 gcttttacgt ccatcaaata taacacttga agagattttt ttatacggag gtaagctgca
    25021 aactgagcag ccaaccaaag aagatgcagc tcctgcagta aataaaaagt atgcgaaaac
    25081 tttttctgga aaggagcaga acccagaata taacaaagaa aaaataagaa aaaaagttaa
    25141 aaataaatca aaaaaagaga gaaataaagg agcaaggcgc aaataagcgc ctttttatgc
    25201 tatgattatg aaaacgggaa caaaattggt tgccagagga ggggttatgt gaaagaacgt
    25261 caagaagaga tcttgcagct attgacagaa aaccaacaag gattgacagc aagtgatgta
    25321 gctgagcgct taactatcga tcgaagcaat gcaagccgat atttaagtga actatacaag
    25381 gcccatcata ttgtaaaaac cgctggccga ccagtcgttt actctttgcc aacggaaaag
    25441 tcaaaatctg atgaagtgca tgtcgactct tctacgcaag tgacttttga aacactcgtt
    25501 ggagaaaatg attctttaaa agtcagcata cagcaggcaa aagctgcgat tctttatcca
    25561 ccgcgggggt tgcatacgat tatttttgga gaaactggta cagggaagtc gatgtttgct
    25621 gaatgtatgt atcattttgc aatcgattca gagatgcttt ctgcagatgc gccatttgtt
    25681 tctttcaatt gtgcggatta tgctcaaaat ccgcaactgc tctttggaca catctttggg
    25741 atcaaaaaag gggcttacac aggagcagct caagatagtc ccggtctaat agccaaagca
    25801 gacggcggta tattgttttt agatgagatc caccgtttac cgccggaagg gcaggaaatg
    25861 ctgtttactt tcattgataa aggtatttac cgtccgcttg gagaaagtgc acaagtacat
    25921 gaagcatctg ttcagatcat tggggcgacg actgaatctt cagaaagttt cttatctacg
    25981 ttcaatcgac ggattcctat ggcaatcacc ttgccgaatc ttgcttcacg ttcactggat
    26041 gagcgttatg aaatcatctc tctttttatc aaacaagaag cgaatcgttt gaatcaacgt
    26101 atcgatatcg aaaaagaagc gattcttgcc ttcatgcttt atgatgcgga aggaaatatc
    26161 ggtcaagtga aacgagactt gaaactagtc tgtgcaaaat cttttttaca ttaccgtact
    26221 catcatgaga aaaagctaat cattcgaaaa gaagaacttt cccttcaagt acaaaaagga
    26281 ttactgaaaa tcaaggaaat accggaacgt ctcgaccgat tcatggacag caaaagtcag
    26341 tatttgacat ttgaaccagg atttgctgat gttgtctggt cacaagatcc agaacgaaac
    26401 atgcaggtct ataacgatat tgaggaaaaa gtcacttctc tttccgaaac cggtgtggag
    26461 aacattgatc tagagaccct catttcaaaa gatgtggatg cttatttcca gacatatgtc
    26521 aaagaattga ctaaaagttc gattcccaaa gatcttttgc cagatgaaat ctggcggtta
    26581 accaatcaat tgtatgatgt ggctgaaaaa cggttagacc gtatttataa tgaaaaagct
    26641 cgttttgctt ttgctttgca tctgcaaagt acattggatc gtgtgagaga aggtcatatg
    26701 atcgtccacc cagatttgaa taacgtacgg aaaaatttta agagggaatt ccaggtagca
    26761 atcgatcttt caacgatcat tgaagaagaa gaacaagttg agattccttt tgatgaaatc
    26821 ggctttatta gtatgtttct ttctatcaat ctaggagaaa gtgagcctgt taaagaaaac
    26881 aaagtagatg ttctcatatt gatgcacggc agacagactg cttcaagcat gttggagatg
    26941 gcacaagaac tattaggaac aaaagtaggg aaagctttta atatgccact ggttatggaa
    27001 gttcaaacaa tgtatgaaga agtgttacaa tatgtcaaaa actatcaaga gcatttgacg
    27061 aatggcctac tcttgttaac agatatggga tcattaaaca ccttcagtga tttgatttat
    27121 gaagagactg gcattcggac gaaagctatc acgatgacaa gtaccatgat tgtgatagaa
    27181 gcgatacgga tggcaagtgt tggaagaagc ttagaagata tttatcaaaa tatccagctt
    27241 tcctttgaat cgatcgtacg tgatcagttc aaagcggaaa agtctgaaac tttacagcgt
    27301 aaaaaagccg taatcgtgac atgctttact ggtgaaggag tagctgccaa attatatcag
    27361 cgtattatgc cagtgattaa tcaaaataag gtagaactga tccagatgca gtttttagaa
    27421 cgtgaatcat tcaaaaaaca catcgatgcc ttattggaag aatatgagat caaagcaatt
    27481 gcgggaacag ttgatgttga ttatcaaaat atcccatttt tttcagccta tgacgttttt
    27541 gatgatgaaa aactaaatat tttaaagcgt atagctgatg atgagatccc gacagctaaa
    27601 attgtcgatt cacttgctgg atcattaagc gatgtctttt ctattgatga actgatccgt
    27661 tcattgcaaa agacagttca gcagatccag accgatttgt cgattatcgt ggaacctgga
    27721 gtagatactg gaatcgttat ccatttagcc ttcatgttag acggattgat caaacaagaa
    27781 agccatcggc aatttgagaa actgtctatt ttctcaaaaa tttatcgttt ggaaatggat
    27841 atcgttcggg caaacttgat ggcactagaa cgaaaatatc aggtaaaaat tccagaagat
    27901 gatatcgcat ttcttacaca aatgtttctt gaaaataaaa taaaaaaatg aacacactaa
    27961 tcatactgtt tacacactaa acatctgtgt gcgcactata aaaaagagct aaaaagcatg
    28021 aaaacgctat ttcagctctt ttttttattg gcatgcttct tgcgttatat aaaagtgtaa
    28081 aaaaataaaa ggagtgttaa aaatggcaaa aaaaacaatc atgttagttt gttcagcagg
    28141 gatgagtacg agtttattgg tcacaaaaat gcaaaaagca gcagaagaaa aagggatgga
    28201 agccgatatt tttgcggtct ctgcctcaga tgcagataat aatttagaat ctaagaatgt
    28261 cgatgtatta ttattaggtc ctcaagtacg tttcatgaaa gctcaatttg aacaaaaact
    28321 tgcgcctaaa ggtattccat tagatgtcat caatatgcaa gactatggca tgatgaacgg
    28381 agaaaaagtt cttgcacaag cagaaaatct aatgaaataa atcaagtatg cagttggggg
    28441 tataacaacg tggaagatca acaaaatcta gaagccatca tggggttaat catgtttggc
    28501 ggtaatgcaa agagcgatgc aatggaagcg attgcggctg ctaaaatcgg tgattttgag
    28561 ttagcggata ggaaaatcgc tgatgcagaa gaatcactta tacaagcgca tcatgcacaa
    28621 acagacatgt tgacacaaga agcacaagga aatcatatgc aagtgacatt attgactgtt
    28681 cacagtcaag atcacttaat gacatctatt gcgtttactg atttagcaaa agaaatcatc
    28741 gaactttatc gtcgcgtata cagcgaataa atacaacttc ttttttgaac ggaagaagaa
    28801 aaatttttga acggattttt tattttgtta taaggagaac tctctttata taaaaaactt
    28861 ataggaggaa gtaaaaaatg aacggactat caagttggat ggagaaatac atcctgccgg
    28921 ttgccgcaaa aatcggagcg caaaaacatt tagttgcttt gcgtgatgct tttatcggca
    28981 cactgcccgc aacaatggcg ggatcagtcg ctgtaatgat caatgcaatt attcgtgatt
    29041 taccgcaaca gtttaacagt acctatgctg acagtttagc agcagacaaa ggaatctttg
    29101 ccgtattgaa tgtcatcatc ggaatcaatg gttttgtctg gaacggtaca ctagcaatcg
    29161 ccggattgat ctttgccttt tcttgggggt ataatcttgc ccgagcttat ggagtaaatg
    29221 atctagctgg ggggatcgtt ggtttagcta ccttgattca aggtatttcc tttggtccag
    29281 cattatcagc agcattagat gtgaatgttc cttcaaatgt cgcagatgca atcaatagtt
    29341 ctgagattgg tgcaacgatt gcagatggca cactgtcagt tggattatgg ggttggctaa
    29401 aactagatca tttgaatgga aacgcttatt ttacagttat gatcatggga gctatttcag
    29461 tcattatttt ttgtaaattg atgcttgcca acattacgat caaaatgcct gattcagtac
    29521 caccagcagt atctaaagca tttgcagcca ttttaccagc gacgatcgct ttatacgtca
    29581 ttgctattat caattatatc gtaggtcgtg tagctgatgg gcaactgatc attgatttgg
    29641 tgcaaaaata tattgcagag ccattcctag gattatctca agggttggga gcagtattga
    29701 tcgtcacagt atttgttcaa gtattctggt tctttggtat tcatggacca aatgtattgg
    29761 caccagtgct agagggaatc tggggacaag cacaactaat caatattgat attttccaaa
    29821 aaggttataa cggaaaaact ggtacaccag ccgtgttaga tgcaatcgaa gatggaaaag
    29881 cttatatgtg ggtccgcgga tcgttcgatg cttttgcatg gtttggcgga tcaggcggaa
    29941 cgatcgtctt actgatagct atcttgattt tctctaaacg tgcagattat ctaacagtag
    30001 ctaaattatc cattgggcct ggtatcttca atatcaacga accagtgatg tttggtctgc
    30061 caatcgtatt gaatgcaatc atgtttatcc cattcttgat tgctccagta gtagcaacag
    30121 caattggatg gacagctact tatcttggcc tagtcgctcc ggtatcgcag caagtcacat
    30181 gggtagtacc gccgttctta ttgtcattct tagcaacagg agcggattgg cgggcaccga
    30241 tcgtaacatt agtatgtatg gtcgtgactt tcttgatttg ggcaccattt gtcatctctg
    30301 caaacaaaat ggaccctgcc gcaggcgaac aacaataaga tgaacgtgag gagcgggacg
    30361 ctttaagttt cgttcctcat aatgaatttt cgaggaggaa ctgaaatgat tacaattgcc
    30421 agtgcaatgc aacaagatga aatcaaagaa ttgttagaag gatacaatga agaaggaatg
    30481 acttttacat ttaaagaaaa acaaggaatc aagctgttct ttgaaacaag tgcagaagat
    30541 ccggaagttg cggctaaaaa agcaaaggaa ctaatcaaag cacagccttg gggaacagtc
    30601 ctttattttc aatcaacagc tgtttagaaa atggtttaaa aagataggat ggttgagatg
    30661 ataaaaaacg gtttattttt atgtagtatt ggttctttga tcgtgttgta tagtttgaat
    30721 ccacattcgc aaaggtatga tttgtatagt atggtcacag gtctttttct aacaggatta
    30781 ggaatctatt tcttcatgaa ggggaaaaaa agagaagaag caaaggagaa aaataactaa
    30841 tggaaatcac gaaaaaatta aatattccag catcgttttt ttacgataaa gttatggact
    30901 ctgtcctttt cgatgtacgt aaagcgaccg gcaaatcagt aactagaaaa cagctgaaaa
    30961 atttggagta cgtcaaacaa ttctcgcaaa ataaccgggc tcgcataaaa atcgaagaag
    31021 tcattgaaaa ccagtcttat aagtttcgta catcgacaac gaagaatgaa ttcgtcgttc
    31081 attatcaaat caaaccattg gacgattcga cctgtgaggt tcattacaca gaaaaaatgg
    31141 agtcttatgg catgcttcaa aagttgaatg atatgctttt aggaacaatc ttgatgtatt
    31201 ttaaacggag acgtttcaaa aaaatgctag atatgatcga agcaacgtat taaatcaatg
    31261 aaacatggta ctatttagtt attgaaaaat gaagtaagct agaaggaatt tttcatagga
    31321 agatagatct ttcctgcgaa tcctcactag cttttttgta ggaaggggca gcaaaatgac
    31381 aaacaaagta agagagctag gtgtatcttc gattgaggaa atgtttgatt tagccactta
    31441 tgcatttaat ggggcagata cacctgaaag gcgagaacgt tttcgtacac ttgcggagaa
    31501 ttcttggaat tacggatttt ttgatgagga ggatcggcta acaagtcaag tcatggcgac
    31561 cccttttcca gtgaattttt acggacaaga gtacctaatg gccggcattg gctatgttgc
    31621 ttcttatcca gaagcaaggg gacaaggcgg gatcaatcag atcatggaaa agatccttga
    31681 agattgccgt gatcgtaaag taagcttatc ttatctggcg ccattctcct atcccttcta
    31741 tcggcgctat ggctatgaac aatcatttga taaaatcagt tatcaactaa acagtcgtga
    31801 tgtgccgttt atcaaaaaaa aatctggaaa aataaagcgt atggattttg aagatgcaaa
    31861 agctgcaatc agaagaattt atgaacagat gccggaaaac caacgagctg gtctgaagcg
    31921 cgaagactgg tggtatacgt ataaattcaa gcaaaaaggc aattaccagt ttgctttgca
    31981 ttttgatgat aatgaggaag caacgggata tatcgtttat cagcttgcaa cgtcttcctt
    32041 tattattgcg gagtggggat ttttggatca tgatgctttt tgcagccttg ttcgcttcgt
    32101 aagttctcac aatggtgctt ttgaaacatt tgaatattca tgcggctacg gcggtaatga
    32161 tcaaaattat ctattagaaa atccatttgc ttctgtaatg atcacacctt atatgatggc
    32221 gaggatcgta gatatttctt tgtttttgga aaattatcca tttaaaaaga gacaagctgc
    32281 atttgcccta caaatagaag aagatcatta tgcgaaatgg aatgaaggaa tcgtggaagt
    32341 aacaatcgaa aatggaaaaa atcatctctc aaaagtagaa caaacaaaac tcccaatcgt
    32401 tcgaggaagt atccaacata tcacgcagct attattaggg tatcgtaaag tggaagagtt
    32461 ggtattccaa gaaagaatac aggtgacaaa agaatatgaa gaaacgttaa gctcaatctt
    32521 gccacagcaa cagccaattc ttaatgacta tttttgataa aaaacgggtg atgacaatat
    32581 ttttgtcacc acccttttgt ttttcatttg gcatttgaag gaaaatgtta aagtaaatcc
    32641 tcttttttcg taaatttgaa ttttttgttt tcttttgttt tagggttttc ttttttgtgg
    32701 tcttggattg cttctttcgt aatcttttcc cgattttttt tagatgaatc taagcgttct
    32761 gcgtatactt tttcttttgg cttatcattc attggaaagg acctccctac attatttcat
    32821 tctacctcta gtttatcatc tgttaaggac tagtggaaac gttttgccta acttataagc
    32881 tgattttttt agcagataag cttgcaagta aaaaacaaac aagctacaat tgatttattg
    32941 aaattgttcc tgtagctcag taggatagag caatcgcctc ctaagcgata ggtcgctggt
    33001 tcaattccag tcaggaacgt acaaataacc cctgtatcct ttgcggtaca gggggttatt
    33061 ttgtataagt agacattgta gtttctaaac tgctatgtcc taaacgtttc gatacgctaa
    33121 gtatatttac tccttgataa agtaaaatag atgcatgcgt atgtcttagt ccatcaataa
    33181 acagtcgaag agaaaatagg gaaaaattga ttactaaaga aaaacttact aggtcgacgg
    33241 tgtagttgat tttcatactt caatagattc gaattaaacg atttcgattg gcttatacca
    33301 gtcctatcca acgaaagata tatcgttctt aactctagcg gtataacttg aagctttacg
    33361 taaagccaat gggaatgaac tggagatgac tttttattat ttttagtaat agctgatggg
    33421 ttaatactta caactttcct tttcaaagta ccatataaaa aaacggtggg gttgtttagc
    33481 gaaataaagg atctaagcca atagcttaga tcctttattc tatgctttat attatttttt
    33541 gcatgtgtgc gaaagtatac tcaagcccaa tcgccatttc ggaaaatcgg tactgtagag
    33601 ccatcttcac ggattccgtc gatatccatc ttgtcagatc ctaccatgaa atctacgtgg
    33661 gtttgacttc tattcaatcc agcttctgct aattcttcat cggacatttc tgtaccgcct
    33721 ttcacactga aggcataagc agaacctagt gctaagtgat tcgatgcgtt ttcatcaaat
    33781 aatgtattga aaaagatgat gccagattga gaaataggtg aagggtcagg tacaagtgcg
    33841 acttctccta aacgacgcgc accttcatct gtatcaagca gttttgctaa gacatcttct
    33901 ccttgttcgg cagaaaaatc aacaactttt ccatctttaa aagtaaattt catacccgag
    33961 atgatggtgc cagcataact cagtggcttt gtacttgaaa tgtaaccatc tactcgatga
    34021 ctgtcaggtg ctgtgaacac ttcttccgtc ggcatatttg ccatgaattt ttctccacga
    34081 gcattatagc ttccagcgcc ttcccaaaga tggtttttag gcaatccgat aatgatatct
    34141 gtaccgggag cagtatagtg aagggcagaa aattgttctc gattcaattc ttctgctttt
    34201 tttgctaatt tttcatcatg cttcttccat gctaagacag ggtcttcttc atagacacga
    34261 gtcgttttga aaatctgatc ccataatgcc tcaacttgtt tttcttctgg cagttctggg
    34321 aagacttttg ctgcccattg tttaccagcc gcagccacga cagtccaact gactttattt
    34381 gcttgagttg ccttgcgtaa attcattagt gctttgccat tcgctgattg ataagacgct
    34441 actcgatggc tatctacacc agcaaatgca tcgggatcgg cagaaacaac gctgatcctg
    34501 cttgctcctt tttcaagcca ttcatccgct tgatcgattt tagattgtgg tacattctca
    34561 atacgatccg ttgcagcatg taaaagaaat tctctttgga tctggtcgtc agtccattga
    34621 acgataacct cagcggctcc tagtttataa gcttcttgtg tgatcaatcg agcaaggggt
    34681 gcctgttcca cgctgatctg taaaacaact gtgtgtcctt tttctgtggc tacgccagtt
    34741 tctgcaatta agcgtgcata tttttttaga agttcattaa aatcagataa cataataaaa
    34801 attcctcctc accatttttt ctggtaacta ttagcatgat accatttaat tatgattcaa
    34861 acaatgaggc tttaaataaa aaaaggaggt tattttttta aggttaagtt tcatgatata
    34921 tgttactact tgctatttca gcttgtgaag aaaatcaaaa actaattttg catatcaaaa
    34981 aaacatgtta caatgaaatt aatcaatggt ggaagcgtta taaaaaaatt aaggagatta
    35041 aaaaatatgg caagaaaaaa gacgattact agagaacaga ttttggcagc tgcttatgaa
    35101 gtagctgcca ccgatggatt tacacgattt actgcgagaa acatcgcgaa caaaatgaat
    35161 tgttccacgc aaccgattta tcttgagttt aaaaatatgg atgacttgag acaagctctt
    35221 tttgagcaga tctatgatca tttacaatat gaagtgttca ctgttgagca tacagggaat
    35281 acgattatcg atcttgcttt taattatatc cattttgcaa gaagagagaa gaagttgtat
    35341 cgttcactgt acttagaaga atatggtgga ggaaaagaga tgcagaattt ctcttatact
    35401 tactttgtga atacagtgaa acgcgatcca gcttatgtga atctttcaga agagcagatc
    35461 gaatctttgt ataatgggac atggatcata gctactggga tcgcagcttt gatgtcttca
    35521 agcatcatcc atccaactga tgaacagatt gaaaagatga tccaagacag tatagatgct
    35581 atcttgaaat tcaaagaacc aatcgaaatc gattgaatat actcaaaagt tccacaaatc
    35641 agtaggtaac aagagatgtg tcaaaataag gaaaagttcg tttttcttac agcgtatgca
    35701 tttttaactc ccgaagaact cttccatgaa tgcaatgaat aaaagagcca tacagattga
    35761 ataaatctgg atggctcttt tattgcaagt atattaaatt tttgcgacta gttctttagc
    35821 gaaagcttct aaattttcga tatcttcttc ttcagcattc agatccactt taacattttc
    35881 ggctcctttt gatgcgcctg tttttgcaaa agcagcatcg aaatcatcca ccgatttgca
    35941 gaattcatcg taaaaagtat caccagatcc gcagacaccg taaatttttc ccaatagatc
    36001 caattcttgc aagtcttcat aaaagtctac gatctcatca ggcagatcgc catcatcgta
    36061 tgtataagta gctactacgc aaatatctgc ctcctcgaat tcatctgcat ctacttgggt
    36121 acattcgttg atttcgactt cgatatccag attttcaaat gcttctgcaa caatatcggc
    36181 gatttcttca gtgtttcctg tcatgctagc atatacgatt tttgctaaag ccatgatgtg
    36241 cctcctcgtt tataattatt taacaattat gaattatacc aaaacatcat tgaaaagaaa
    36301 ataaaaccta aagacacctg gggaatgtca tttggctaaa tgtatgccgc tatgttaaaa
    36361 tgagagggat ttgtaaaaag gagacgaaaa tatgattact ttaaaatcag cacgtgaaat
    36421 tgaaatgatg gcagaatctg gagctttatt agcagatgta cataaaaatc tgcgtgattt
    36481 tataaaacca ggaatcacaa gttgggatat tgaggtattt gtcagaaact atatcgagag
    36541 tcatggcgga attgctgcgc aaattggctt tgaagggtat gaatatgcga cttgtatcag
    36601 tataaatgat gaaatttgtc atggattccc tcgtaaaaag cctttgaaaa acggtgattt
    36661 ggtaaaagtc gatatgtgta tcgatttgaa aggcggcatt tctgattctt gttgggccta
    36721 tgtagttgga gaatctacac ctgagatcga ccgattgatg gaagtaacga agaaagcttt
    36781 gtacatcggt attgagcaag cacaagtagg gaatcggatt ggtgacatcg gtcatgctat
    36841 ccaaacttat gtagaaggcg aaaatctagc gatcgttcgt gattttattg gtcatggcgt
    36901 tggtcctacg attcatgaag aaccagcagt tcctcattac ggagaagctg gaaaagggtt
    36961 gcggttgaaa gaaggcatgg ttatcacgat cgaacctatg gttaataccg gaacttggaa
    37021 aatgaaaatg gatcctaacg gctggactgc gtatacaaga gacggcggac tttcttgcca
    37081 atatgaacat acattggcta ttactaaaga tggtccaaga attttgacat cacaaggaga
    37141 agaaggcact tattgatcta tccagtcaat aggtacggga gaggtacatg aaagtttgga
    37201 acaaaataaa acaaaatgaa aatctgatgc gctttattga gacgactcaa aagcgcatca
    37261 ctgattcaga aatgagtacg acctctgtcg tcgttgctta ttatttactc ttgtctttat
    37321 ttccattgat tattgctttt ggtaatattt taccgtttct ccacatcgat caagagactg
    37381 tactgaccta tttacgagaa gtgattcctg agaccgttta ccagtttatc ggtcctgcaa
    37441 tcaaagacct tttgacacaa agttcaggag gcttgttatc cgtttctgca ttagcggctt
    37501 tatggtctgc aagtcaaagt atcaatgctt tgcaaattgc gatgaataaa gcgtatggcg
    37561 tagaaaatag aaaaaatttt ttgatcgtcc gtttcttctc actaattgtc attgtcttgt
    37621 tcatgattgc tattagtggg gtaactatcg ttcttgggtt gggacagctg attttggatg
    37681 ctattcagcc aatcgtacaa atacccgtga actttatcga tcagttccaa actttgaagt
    37741 ggccaatcac attggtagct ttatttgtca tcatgttctt gatttattta attgtcccta
    37801 atgctcagtt gaagttgaag acgatcattc cgggcacgat cttcgctact gtcggctgga
    37861 tgctgttatc acaagtgttt agtatctatg ctaggtattt tgcaacgagg atcagcggtt
    37921 atcagatcat aggaagtttt attgtactca tgctttggct taattttgct gctacgatca
    37981 tcattttggg cggaatcatc aatgcagttg ttcaagaata cgtgacgggt catcaaatca
    38041 aaaaacgtcg agatccaagc cgaaaatggt tcaataaatt gaaaactaaa ttttctcata
    38101 aaaaaaagta gaaacaagag ttcttattgt aaatttatat cagtaattta taataattat
    38161 gactcttaat taaagtaggt gcccatatgc ttgtttcttt tagtatggtt ataagatttt
    38221 ttctgacaat tttattatca ctgatattaa cacctgtatt caaaatcatt tcagtacaaa
    38281 caggtatggt cgacaaacca aatgaaagaa gaataaataa agttccaatg ccttcagcgg
    38341 gcggactgcc tatctttatt tcatttgtta tatcgacttt attcttattc cgtaaaatta
    38401 tccctcaatc gtatattgta ccgatcttga ttgcttcttt agtaatcata gtcacaggag
    38461 tgctcgatga taaatatgag ctgacaccta aacaaaaaag tatagggatc ttgattgctt
    38521 ctcttattat ttattttgta gcagatatac gtattgattc cttcacgctt ccttttattg
    38581 gatatatcca actaggctgg cttagttttc ccgtaacgat tttttggatc ttcggtatca
    38641 cgaatgcggt caatctgatt gacggtttgg atgggctggc ggcagggatt tcattgatcg
    38701 gtttaattac gataggaatc attggatatt ttttcctcca tgcttctaca gtgtatattc
    38761 cagttgttat tttctgtctt gtagcaagta ttatcggttt ttttccttac aatttttatc
    38821 cggcaaagat ttatttaggc gatacaggag ctctattcct agggtttatg atggccgtcc
    38881 tttcactaca agggctgaag aacgtgactt ttgtttcatc gatttctttg ctgatcgtca
    38941 tgggagtgcc ggtaacagac acgttttttg ctatcattag gagaaaagca aaccgtgttt
    39001 ctttttctac agcagataaa aaacatcttc atcaccgact aatcagttta ggctttactc
    39061 ataaaggtgc tgtcctgacg atttatgcga tggctttgat gttttctttc acagctatgg
    39121 tcatgaatta tactggaaga atcggaacga tcgtattgat tattgccatg ttgtttgctg
    39181 caattttatt atttgaattg attggtctga tcaatgagaa acatcagttt attttaaaaa
    39241 cattgcgatt tttagggaat aaagagtacc gtacgcaagt aaaacagaaa tatttgaaga
    39301 aatggaaccg tcatatagat gaataacaaa ttaaaccagc cgatccttat tagtattgtg
    39361 acggcaaaca gtgataaaat ttttcggaca ctagataatt ttatttcaac tgtaaaagat
    39421 gaaacagcag taaaaatccg aatttttgac aacaattcac ttcccgagta caaaaaaaga
    39481 ctaacagagt ataggaaata tccctacatc gatatctatt ttcatgatga aaattccgga
    39541 tttggttatg gacataatca caatctgata gctagcgatt ctcgctatgg cattatttgt
    39601 aacccagata ttttagtgga tgaagagaca gttacagcat tggtcttgtt attgaaagaa
    39661 catccggaat gtgcgatgtt agcacctaag attcttaatg aagatggaac aactcagcac
    39721 ctcatccgtg agcagctggc tgtttttgat tacgttttgc gctttgtgcc tttcaaatgg
    39781 gtcaaaaaaa agtttgacaa acgactggct gtgtttgaat gccgaggatt gcctactgat
    39841 gctaatagtt atgtacggat gatctcaggt agttttatgg tagctgatat tgataaattt
    39901 aaagagataa aggggtttga tgagcgtttc ttcatgtact ttgaagacaa cgacttgtgt
    39961 ttgagttttg aaaaagcagg ttacaagtta ttgtacacac ccttttatgc agttatccat
    40021 ttttacggga agggtgcgca tcgaaatgca aaactgtttc gcgtgttttt gacttctatg
    40081 atgaaatttt tcaataagtg ggggtggcgt tttttctgat gaaaccagaa atattagtag
    40141 tcgttgtgct ttatcagcaa ctattcagtc aatctccagc ttatggacca ttaagcaaag
    40201 cacttagcga aaagcaaatt cagcttatta tttacgataa tagtcctcaa aggcagcaaa
    40261 atgagctgtt tgaaaaagaa aatatcgttt atcatcatga tccgcaaaat cctggtttag
    40321 cggtggctta taattttgcg ttgtctcaag cagatgaagg aacacgttgt ctcgtcactc
    40381 tggatcagga tacgaggcta gtcgacgatt attttgaact attgcgtaaa gtaaccttta
    40441 cagatgaatg tgttgctgca gttccgatga ttttttctgg gagccgacaa gtttctcccc
    40501 tatattcaga tcgttatatc aatcgtacag ctcaagcagt agaagttgaa ggcccaacag
    40561 ctgaacgaat catggcaatc aattcaggtg ttgcctggtc tgtcaaattt ctaaaaaaga
    40621 ttggcgggtt taatcctgct ttttctttag attttttgga tcattggctg ttttggaaag
    40681 tggatcagtt ggaaaaaact gtggagatcc ttcctgtacg aatggaacat gatctttcgg
    40741 ttttagacta tgaaaaagtt tcaagtaatc gataccgttc tattttacaa gcagaaaatc
    40801 gattttatca tgaatatgat caaaggcatt tatccagtca ccgacgacaa ttattgttaa
    40861 gaacagctaa acagttcttg accgtcaaaa accggcatat ctggcggatg actttgcgct
    40921 catatgttaa gaattggaag gtgtgaattc gtgcgttcgg tttgtatagc tacctttaat
    40981 ggaggcagat atttaagaga gcagttagat agtattttat ctcagctttc agcagaagat
    41041 gaagtagtca tttctgatga tgggtcagta gatgatacat gggatatatt aactgaatac
    41101 gcagaaaaag ataagcgaat ccagcttttt tcggggcctc gtcaaggatt gatcgctaat
    41161 tttggttatg cgatccagca gacgaaagga gatctaatct ttttggcaga ccaagatgat
    41221 gtttggttgg agacaaaagt gaaaaagatc actgactatt ttgagcagaa tccggatcag
    41281 ctagtagtag tttctgattt aatcattgtg gacagcgagc tgcaagagat ccatgattcg
    41341 tatttttcgt ttcgaaattc caaagaagga tggcttaata atgtgataag aagtcactat
    41401 attggtgctg gtatggcttt ccggtcttct ttaaaacccg atattttgcc gatcccgtca
    41461 gaagtgccga tgcatgatat gtggattggt ttgctgggag gaaagaagac tgggttccta
    41521 agagaaccgt taactctgta ccgaagacat gatttaaacg cgagtgagat tgaaacacat
    41581 tctaaattac ggcaaaaaat ttcttggcgt gttcatctga ttacggcgtt gatcaaacga
    41641 aaatggttta aacgttgata aaagatgttt aaatccttaa tatttccatg acaaatataa
    41701 caattgatga gataataaca aagtgaaatt tgaaatcaaa aaggagagca gatatccatg
    41761 aagggaatca ttcttgctgg gggcagcgga acacgtctgt accctttaac aaaggcaaca
    41821 tcgaaacaat tgatgccgat ttatgacaaa ccaatgattt attatccaat gtctactctg
    41881 atgttggcgg gaatcaaaga aattctgatt atttcaacgc cacaagatac accacgtttc
    41941 aaggaattat ttggaaacgg aaatgattta ggactgcaga tcgaatatgc agtacaagaa
    42001 agtccagatg gattggcaca agcgtttatc attggagaag aatttattgg agatgatagt
    42061 gtctgcttgg tattagggga caatatttac tatggtggcg gtctgtctaa aatgttgcaa
    42121 cgagcagctt caaaagatac aggcgcgaca gtatttggct atcatgtcaa tgatccagaa
    42181 cgctttggtg tagtagaatt tgatgagaat atgcaagctt tgtcgattga agaaaagcct
    42241 gctcagccaa aatcaaatta cgctgtaact ggcctttatt tttatgacaa tgaggtaatt
    42301 tctatcgcta aaggtatcaa accttcagaa cgtggagaac tagagattac ggatgtcaat
    42361 aaagcatatt tggataaagg aaaactatcc gtagaactta tgggacgcgg atttgcttgg
    42421 cttgatacag gaacacatga atcattgctt gaagcatcta cctttatcga aacaattgaa
    42481 aaacgtcaga acttaaaagt ggcttgtttg gaagaaattg cttaccgtat gggctacatc
    42541 gataaggaac aattgctggc ccttgcacag ccattgaaaa aaaatcaata cggtcaatat
    42601 ttattgaatt tggccgctga ataaggagtc cttacagatg aaagtaacag atacaaaatt
    42661 aacagatgtc aaaatcgtag aaatggacgt ctttggcgat catcgcggtt tcttcacaga
    42721 aagttattca aaagcaaaat ttgcagaaca tggattgaaa tatgactttg ttcaagacaa
    42781 ccattcacta tcagctgaag ctggtgtcct tcgggggctt catttccaaa aaggagaagc
    42841 tgcacagaca aaattgattc gggtagtcac tggagcagtt ttagatgtag tagtggatat
    42901 ccgcaaaggt agcccgactt atggtcaatg ggacggattt atcttatctg aacataatca
    42961 tcgacagttg cttgtaccta aaggatatgc ccatggattc gttactttga caccaaatgt
    43021 gaacttcctt tataagtgtg ataattacta taatgcagaa gcagatgggg gcatcgcatt
    43081 tgatgatcca gatttagcga ttgattggcc aattgatcca tcaagagcga ttatgtcgga
    43141 aaaagatcac cgtcatccga cattaaaaga atttgaagca gaaaatccat ttgtttacgg
    43201 agaaatctaa gaattaagga gaaattttct tatgaaaaac atcattgtta ccggaggtgc
    43261 cggattcatc ggctctaatt tcgttcatta cgtggtaaac aatcatccag aagtgcatgt
    43321 cactgttttg gataaactga cttatgcagg aaacaaagag aacttggccg gacttcctag
    43381 cgatcgcgta gaattggtcg taggagatgt tgcagatgca gaattagtgg accgtctagt
    43441 aaaagaagcg gacgcagttg ttcattacgc ggcagaatcc cataacgata attcattaaa
    43501 agatcctttc ccatttgtac aaacgaatat cattggtacg tatacacttt tagaagcttg
    43561 ccgcaagtat gatgtacgct atcatcacgt atctactgac gaggtttacg gcgatcttcc
    43621 attacgtgaa gacctgccag gacatgggga aggcgaagga gaaaaattca ctgcggaaac
    43681 accatataat ccttcaagtc catattcttc tacaaaagct ggttcagatc tgttagtaaa
    43741 agcatgggtg cgttcttttg gcttacgtgc aaccatttca aactgttcaa acaactatgg
    43801 tccttatcag catattgaaa aatttattcc tcgccaagta acgagtatct taagcggtat
    43861 tcgtccaaaa ttatatggcg aaggaaagaa tgttcgtgac tggatccata cgaatgatca
    43921 ttcttcagca gtgtggctga ttttgacgaa aggcagaatt ggcgaaacct atctgatcgg
    43981 tgcagacggt gaagaagata ataagaccgt gatggaattg atcttagaaa tgatgggcca
    44041 accaaaagac gcatacgacc atgtaaacga ccgcgcaggg cacgatttgc gttatgcaat
    44101 cgattcgaca aaattgcgag aagagctagg ttggcagcct gagtttacga acttccgtga
    44161 tgggttggct gacacgatca aatggtacga agaacatgaa gactggtgga aaaaagaaaa
    44221 agaggccgtt gaagcagcat atgccaaaaa tggacaataa tacagaggaa atttcactgt
    44281 attaatggaa aaacacatgt gaggcaggcg ctcgcctgtc tcttttttaa aataaaaatc
    44341 aaggagcgga taaccatatg tttttattaa ctggaggaaa tggacaatta ggtacagaat
    44401 tgcgccattt attagatgaa aaaaatctgc catatgtttc aacagatgca aaagacttgg
    44461 atatcacaga tgctgagaaa acaatggctt atattacaga attaaaaccc gatgttattt
    44521 tccattgcgc agcttacaca gcagtagata aagcagaaga agaagcaaaa gaattagatg
    44581 agaaaatcaa tgtcgatggc acaagaaatg tagcacttgc agcaaaagct gtgaatgcac
    44641 aattcgtcta tatcagtaca gactatgttt tcgatggaaa aaacaaaaat gtagaatacg
    44701 aagtagacga ccaaacgaat ccactaaatg aatatggtcg tacgaaatta ttaggcgaaa
    44761 aagcagtaga agagatttta gaagactatt atatcatacg aacttcttgg gttttcggga
    44821 tctatggaca caatttcgtc ttcacgatgc aacgcctagc ggaaacccgc gacaaactga
    44881 ctgtagtaaa tgaccagttt gggcgaccaa cttggacacg tactttggca gaatttatgg
    44941 tatatgtcat cgaagaaaaa gcaccatttg gtatttatca tttgtcgaac gaaaatagct
    45001 gttcttggta tgagttcgca aaagaaatat tgaaagatac agatgtagaa gttgcacctg
    45061 ttacgtcaga agaatttccg caaaaagcaa cacgtccgca gtattcagtc atgagcctga
    45121 aaaagacaga agcgttaggt tttgtcatac caacttggca agaagcactt gcgcaaatgc
    45181 tggaagctgc caaaaaaatg aataagtaat ttccttttta tcaaatagaa tttggagcat
    45241 gatatggaaa aacaaacaag aagtgcacga cgaaaagaaa agccaaaagc gaatcaggca
    45301 gattcgaatt tggtgcttga cctgtttcta aaactccgct atcttattgg cgttttagtc
    45361 atcgtggtca tcgttacttt taatttaaac ggaagttcac ttggtgtttg ggataaatat
    45421 gtgtcgcaaa gagacgatgg gaaaaaaact gatgtgattt ttggacagaa tcgggaagtg
    45481 cggtcagacg aatggctggt gcagacacct ttttacctct cacaagctga agaggacttt
    45541 tcgttggtga acgaggatta ttcattaaac ggacaaaaca tgatcattgc gtataattct
    45601 cctgtaaagg atattacggt tatcggaaaa ccattcaact ggggattctt tttcctcgga
    45661 agagatcgag gactttcctt ttattgggcg atgaaattag tcgtcatggt cttgcttgga
    45721 ttcgaagtac tgatgatatt aacgaagagg aaaaaagctc tatcgctaat cggtgcattt
    45781 gcgctgacat tttctccagc agtccagtgg tggttcatgc aacatgtggg cgatttgatt
    45841 ttctttactt tgggactgat gattgctttt tatcattatt tctacaatca tgataaaaaa
    45901 tggctacgaa cgttgatgat gcttttggtg gttattttcg gtattgggtt tattttagtg
    45961 atttacccag cccatcaagt gatgctggcg tacttgctga ttttttattt tgttggcctg
    46021 cttatttact atagcaagaa agtttcatgg gatctgttgg atgtagtgtt gattggaggg
    46081 gcagttttat ttattggcgg agtcatggtt catttctggc taacgtcaaa agacgcattg
    46141 ttagcttctt tgaatacctt atatccagga aaacgggtaa gtactggagg aaagtggacg
    46201 attggcgaat ttttttcttt ccttacaaat tggaaaattc cttttgaaga tatcaactat
    46261 tcgaataact ctgaagtggc cctgttctat cacttttttc caactgtatt cctagcaagt
    46321 ccatttgtgt tatttggcaa aaaagatagc gaacaaaagt tactgggacg tattttgatg
    46381 attttttgtc tgttttctat cttttggatt accgttgggt tgccaaaatg gctagcagaa
    46441 atcactctgc tgtcttatgt tcctccatta agagccatcc ttacttttag ttttgcggct
    46501 tgtttattat cgatctggtt tatcgcttat atttggagag aagatgttat tcctatttgg
    46561 tataaattca ttttgttggc agtaaatgca tggtcttatt tctatgcgat tacgcattcg
    46621 aaaatggata attatttttc ggattttgaa accatcgcag ttgctgtgtt gggaacagtg
    46681 attttagctt ttgcattatt tggctggaga cgcttgtttt acctcgcttt tgtcgcctta
    46741 gttatcgtat ctggcttttt cgtcaatcca gtcgtagaag gtacaggagc gatttttgaa
    46801 aaaacactgg caaaagaaat ccaaaaaatc gatcaacaag atcctggtaa agtttggttg
    46861 tctgaagatg aattatataa cttcacccca acattgggag tgaaatcctt caatacagta
    46921 cgcttttatc cagatatagc tgcatggaag aaaatcgatc cgaaagaaga atacgagaag
    46981 tattataatc ggtatgctca tacaagagct tttatcgcta aggaagatac aaagttcatt
    47041 ttagatcgtc cagatatgtt ttcggtcacg ttgaattttg aagacgcaga aaaattaggg
    47101 atcgactatg tgatcagtaa acgaccatta actgattaca accaattata tgaagcgcag
    47161 ttcacgcagc tatatggacc ggataaagac ggttatatga tttttaaagt agaatatccg
    47221 gattatccag atgtaccaca aacacaaact caggaaaatc agctagtcgt acagtaatag
    47281 gctagctgag aataatcctg aaatattagt agaaaggaga gtgaagataa ttcaagcaat
    47341 cgtacaaaaa tttgtcaaca aaatgagtgt cggtttcttg cttttgttgc tgacgctagt
    47401 cttttttgtt tggacagcag gggtaggttt tccagcaact ttgtttatat ataacaatag
    47461 ttcattactt atcgctttta gtgtggctat tttgctcatt ttaaatgttt ataaagcaaa
    47521 aggcgtcgat tttctttatc taggtgttgc gcttgccctt ttcattttct tttcttactt
    47581 tcgggaatta agaaatgagt ctttatttgg agacttactg tttcctttgg ttattttatt
    47641 ttgtttagta attaaatgga tcgagtggac aaaatgggat cgcggtattt atctgattgt
    47701 ctttacggtc ttcctcggaa tcacgttata tcgtgtattt acagagatcc aagtaccaga
    47761 aggacaaagc atctgggctc caggcaattc gctttctgat atttggatca atacgaatac
    47821 aatcggaagt tctttgatga ctcttgcttt attgatcagt ggatttgcca gttcttttga
    47881 acgatggtat gttcgaatca tcggtgggat agcagtcgtt gcagcctttt taggtatttg
    47941 ggtctgccaa tctagaggtg cattagtcgc gttgattgtt tttgcgatat tggatctttt
    48001 acctaaatcg ttgatgcgtg tcattcgtgc gccatttatt gcctacacgg ttacgattct
    48061 tttagctttg ccgatttctt atctggcggc tgtctctgaa aaagtcaatt tgtttaccga
    48121 tcgagaagat atttggcata aattctatca gacgctagga gaaaaatctg aacaagtttt
    48181 actaggaatg aaaacattta tcttccagcg aggaaaccaa tttttaggaa accacaatag
    48241 ttataattct attttgaata tctatggatt gataggattt gggattgctg ctttgctgct
    48301 tatccttttt attggtcgct taacgttgaa agcagattta tccaacgggc agatgacttt
    48361 tatctgggcc ttttttgcgg tgatgatgca gtcatttatg gaggatacat tgacttcata
    48421 cccatgggtt ccgattattt atatcctgtt ggctatggcc gttcatcgat atgatgaacc
    48481 agaagttgcg gaggaattct atgttatgga agaggcatat gacgatatga cttatgatga
    48541 tacatcttat gaggaacaac cagatagcgc tcattcttcc aggatccaac gctatcattg
    48601 ataggcacaa aatacgagag aggctgtgac aaccgtcgca gccttttttg gtttattgca
    48661 attgtctagc atcttctgta aaatagaaac gttagagaaa atcttgtatc atagaaaatt
    48721 aagaaagaaa taagaacgca gctcggtacc aaaaatatgg taaaatggca cttgactgta
    48781 attcactgaa aatcgcttgg aggaacacaa atgtttaaag atttgtattt atttatgaag
    48841 gacatttatc agaacagaaa attattgcta cagttttcga tcaatgactt caaatcaaga
    48901 tatgcaggat cgtttttagg aatcttatgg gcgtttatca atccgctatt tactgtattg
    48961 atttattggc tggtatttgg tgttggactg aaagcacaaa tgatcgatgc aaaataccca
    49021 tttatcgtat atttagttac cgggatgatt ccctggttct tcttctcaga tgcatttgca
    49081 tcaactacgg ttgtttttcg ggaatatact tatttagtaa agaaagtcgt attcaatatt
    49141 cgtatcttgc ccacgacgaa aatcctatcc aatctgtaca cccatttatt cttcattctg
    49201 attggcatag ctgtcgcgct tataaatggt atatatcctt cagcaatgtc tttacagctg
    49261 atttattatt tattttgcat gtgtgctttc ttgacaggat tgacttggct gacggcatcg
    49321 atccaaccat ttttacctga tatcatgcaa ttaattacga tcgtgctgca gttgatcatg
    49381 tggacattac cgatattatg gagccctaat cagtttgatt caggtcttgt aagcatattg
    49441 aaattaaatc cgctgtacta tgttgttcaa ggttatcgtg agtcattttt aagtgaagct
    49501 tggttctggg agcatggaac atatactttg tatttctggg ttttcacgtt agtaatgttc
    49561 gtaatcggct ctgcagtgtt ccgtagattg aaaccacatt tttcagatgt gctgtaagga
    49621 gaatagctat gacagaatat gcaattagac taaaagatat cacaaaatct tataatatgt
    49681 acgcaaaacc ttcggatcga tttcgtgaag ccctcaatcc ttttaagaaa tcctatcatg
    49741 atgtatttta tgcgttgaaa aacgtgaatg tagaaataaa aaaaggtgag atgatcggtt
    49801 tcgtgggaga aaacggatcg ggaaaatcta caatgttgaa aatcattacg ggagtactga
    49861 caccaacatc cggcacgatg gaaatcgaag gaaaaatctc agctttgcta gaactaggat
    49921 caggattcaa tcctgagtat tctggctatg aaaatatcta tttaaatgga atggttctag
    49981 gattttcaag agaagaagtc gatgaaatgg tcgatgatat cattgaattt gctgacatcg
    50041 gagaccatat ttatcaacct gtaaaaactt attcaagcgg gatgttcgtt cgtctagctt
    50101 ttgctgtagc aatcaatgtg aatcccgata ttttgattgt ggatgaagca ttggcagtag
    50161 gcgatcttga atttcaacta aaatgtatgg aaaaatttac agaaatcaag aactcgggaa
    50221 agacgatttt attcgtttct catgatatca gttcgattcg tcgtttttgt gatcgttcct
    50281 attggttgaa aaacggagaa gtcgtcgaat atggcgatac gatggaagta acgacgaatt
    50341 atgaaaactt tttaaagaaa aaatcagtta aaacagtgga tcggaataaa aaccaagtag
    50401 aacggttcgc cgctccagat atcgtcgacg tgacgaaagc ggaattgttg gataaagaca
    50461 aaaatccggt ggatatgctg gaacaaggag aaaaactata cgttaaagta acttatgaag
    50521 taaaagatga tacaatcaaa aatccggttt taggaattgc gctacgaaca gtcgacaatt
    50581 tttatgtatg cggacttaat actctactag atggtatcaa gattccttgg aaaaaaggag
    50641 agaatatctt ttatctggag tatgaaaagt taggtttgtt agctggtgaa tattattttg
    50701 acgttgcggt ctttgaagaa aatgcaacgg ttcctttggt atataagaca aagtacatga
    50761 acttgtttgt cagcggttct tatatcggag aaggcatcgt ggtcttagac cataaatggg
    50821 aagaagggac tcatagcaat gaaatatgat tttgaaatgg acctagatga acaaagcagt
    50881 gtgggaaaaa ttgccgcaca gatcaaacca ggaagtaaag ttttagagtt tggtcccgga
    50941 aacggccgat taacgaaaca tttaatcgga gcaaaacaat gtgaagtgag cattgttgag
    51001 ttagataagg aattgttcga ttttgtcagc gaatttgctc aagatggatt ttatggtgat
    51061 atcgaaagtt ttgagtgggc aaactattat gctggacaga catttgatta cgtcttgttt
    51121 gcggatgttt tagaacattt ggtagatcct ggaaaaacat tgaagaaagt cagagaattt
    51181 ttgaatgaag aaggagagat cctgattaca ttccctaact tggcgcataa ttctgtcatg
    51241 atcgatttgt tcaataacca attgccgtgg gcttcttatg gtctcctaga tgaaacccat
    51301 aattcttttt atactcatga tggcttccaa aaagtttttg aaaaagcggg attattcatt
    51361 aatattgaag attatttgta cttagctgta ggtgatacag aattaaaatc aacttatgaa
    51421 gaattgccgg aagctgtacg ctatgatttc aaaatgcgac catttggtga agtctaccaa
    51481 tactttttct cattgatgaa acatccagta gcacaaagtt ccattgcaga gccacaaaat
    51541 tctaattatg taaaagtgtt agaagtaacc cagcaaacga aacaagatga aacaatccaa
    51601 caaataccat tcaataattt cacaggggaa aatgagacac taagtttccc cgtatcagaa
    51661 acaacagaaa gaatggtgtt tcgcttcgcg caacaaccta gctttatcga attcagcgca
    51721 gaagctgccg gagaaaaact aacatttatc gattcgaatg ctgtagtcaa aacagtaaat
    51781 gattgttatt tgtttgatgg gaaagaatta ccagagttcg ttctaacgga tatttctggg
    51841 aaagaagtga ctatccattg tcactatcgc tttatcgggg aattgacacc aacaatgaaa
    51901 gaattgttgg aaacgattcg tcccatggct gaagtcacaa agcagctttc tgagaaaaat
    51961 gacgaattag aaagagaaaa tcatcatctt cttgaggaaa atacaagact agaccatacg
    52021 ttaaaaacaa ccacaaatcg ctattgcaca ttgttggaaa gtgatgaatg gacaataaaa
    52081 catagactag gacgacgtaa gaaagaaact tcgaaaaaaa tccaagaaaa agaactttct
    52141 atatgtattg acgagaaaat ctgggatgca gaaacaaaaa tattaaaaat catcggatgg
    52201 ggaatcgcta attcggatcg ccagcccttg tcctataagt tatcagctga ccaatcgcca
    52261 tttttcgaag caattcctgt ttcacgagaa gaagtcaatc aagcagaaca gctcgcaaaa
    52321 ggaacagaag cgggatttga gttacgaatc ctttgcgagc aagaacgttc attcttagtg
    52381 gaagcagtag ctcaaaatgg ccaatcatgg atcattgaaa tgtaaataat gattaactta
    52441 taaagataag gagtaaacga atcgtgcaaa aaaaagaaat tgccgtattt atcgatcaaa
    52501 tcactcgtga acgggccact ggcgatttgc ttattgtggg ctgggcaatt gatgaagtga
    52561 ccaaagaaat ccctacgata aaggtagaaa aagaaaatgt gattgctgaa gcaacacatg
    52621 tcgtaagatt ggatatcaat catttatata atcttgatgt taagacccaa agcggtttca
    52681 aggtacgttt atcagggaaa atgagaggaa aagccatctt agacttccaa acagcgagac
    52741 atcaaaatgg catagctgtg aaattaaacg gtaaataccc ttatgatgac ggcattgaat
    52801 cttcatggga aagaaaaaaa agattactga aaaaagggat aaattatgca aggactcatg
    52861 gcgtaaaaaa agcgatccgt cgggtcaaat tggaattgaa gccaggatcg atcgactatg
    52921 cagagtggat cagtcgtcat gagaaaccag atttgaaaga gcaaaggaaa gaagcacaag
    52981 gatttgcgta ccgtccattg atttccatac ttatgcctgt ctataacgtt gaaatcaaat
    53041 ggctcgaaaa atgcattgat tcggtactgg atcaaacgta tgatcattgg gaactatgta
    53101 tcagtgatga tgcttctact gatccagcta tccgaaagtg tctggaatcc tatcaagcaa
    53161 aagatgatag aatcaaagtc gttttccgaa aagaaaatgg acacatcagt ctggcaacta
    53221 attctgcatt ggaaatagca gaaggagaat ttatagcact cttagataat gatgatgaat
    53281 taccgccatt tgcattatat gaagtggcaa aagtgctaaa cgtacatcca gaactggatg
    53341 tgatctacag tgacgaagat aaaatcgatg cagatggcaa tcgctttgat ccgcatttca
    53401 aagcagactg gtcgcctgat acattgatgg gaaataatta tatctctcac ttaggcgtat
    53461 atcgaacaag cattgttaaa gaacttggcg gtttccgtaa aggctatgaa ggttctcaag
    53521 attatgattt ggtccttcgt gtgacagaac aaatccctgc agatcatatt taccacattg
    53581 accgagtctt gtatcattgg cggacgatcc caggatcaac tgcaagtaat ggcgaagcca
    53641 aaagttatat ctatgattcc ggagtgaaag cattgacaga tgccctgtct agaagaaaca
    53701 tcaaaggaag cgtacgtcct ggcagaattt ctggatttta tgagattgct tatgacgtct
    53761 tgcaagagga tttggtcagt gtgatcattc caacgaagaa tggctatgaa gatctaaaaa
    53821 cttgtgtgga ttcgatcatc gaaaaaacgt cttattcaaa ttatgaaatc attattgcgg
    53881 ataacggaag tacagatcca aaaatgcaag aattatttgc agaatacaag catcagctga
    53941 aagaccgctt tatcgtagaa ttgattgaca ttccattcaa ttattctcga attaacaatt
    54001 tagcagcaga aaaagcaaac ggcaaatact ttttattctt gaataacgat acagaagtga
    54061 ttgagccaga ttggatgacg gtgatggttt cttatgcaca gtttgatcga atcggctgtg
    54121 taggagcaaa actgttttat ccagatgata caacgcagca tgcaggagta ttattaggaa
    54181 tcggtggtgt tgcagggcat gcattgaata actatgatcg aacacattgt ggttatttcg
    54241 gccgattggt cattgacgtc aattacttag cagtcactgc agcttgtatg atggtcaagg
    54301 cagctgactt caatgctgtt aacgggtttg atgaaacatt agaagtagca ttcaacgatg
    54361 ttgacttgtg cttgaaagtt tatgaactgg gtcgttacaa tgtctatgca catcaagcag
    54421 aattgtacca tttcgaatct aaatcaagag gttatgaaga tactccagaa aaacaaagac
    54481 gttttgctgg tgaaatcaaa aaaatgcaag ataaatggcc agcatatatt gcgcatgatc
    54541 cattttacaa tgataattta acaaaagacg gaatcggcga cttttcgttg agaccagatt
    54601 aaactataat aaaaagctgt tatgttgtga ttcgctcgca cataacggct ttttatagtt
    54661 ttttgtcaaa aaaaggcaga aggtgccgac tcaaaaaaat aagtcgaggt tgggacagaa
    54721 gtgtttaact ccgagaaata agaagaaatt cacgaaaatt gcttttcaaa tttttgtgaa
    54781 tttcggctta tttaagaaga agttgcttct gctcccaccg tttattcgtt tttaaagaat
    54841 gagacaaaag tgtttcttac ttttgtccca cgctcttatt catgctcaga gcgccaatag
    54901 atacaaaatc atagccaaag tttcacgaac cctagctgtc aaagaatcga tattttattt
    54961 taatataggc tattttaaaa taaatggttt aaggtattcc tctacaaaag ccactctatc
    55021 aattatcatg ggtttaaaat aagaagtgat ggagataacc agattgttct tagcgtcaat
    55081 ataaagaaca tttccactat taccaattgc tgaatatatt tcgttctcag aatctaatac
    55141 ccaccataaa tagccatatc tcatattttg taagtcttct aaagcactgg ttctaggttt
    55201 gaacatctcg tttatccaca tgggtggtac gatttgtttt cctttataca tccctttatt
    55261 taggcataat acaccaattt ttgccagatc acttgccgat aaacacaaac catatcctgc
    55321 cgtcccgata tttagtggat cacaaaacca gacattggtt ttaggagact tatccattgt
    55381 aaactgctta tgttcttcag ctgtttcagc ataataattg acatggtttg gtatccctat
    55441 aggatcaaat agatacttat tggcaaaatc aacagttgtt agcttacttg tctgatacaa
    55501 caagccagtt aatatgtgta aacagacagt ttgatagttg aattcgccgg ttataccttt
    55561 tcttcctccc aataaatcta gtgatgtttt tgtccaatct tcacttgaac agacttttgt
    55621 ccatggatcg cctttacatt tgtagggtgc tttcattgtt agcaagtgct tgattgtaat
    55681 agcgtaaata gttttctccc cgcgtttcac tttatactct ggaaaataat ctaatacttt
    55741 atcatcgata cttttgatta atccttgctc taaagcaatg ccaatcagca aagatacaat
    55801 acttttcgtt gctgacatga tatgacaagt atcgtctttt ttataaccat tccattcatc
    55861 tgtataaact tctaccccat ccttatatgc gacaatttga catatatttg gttgtcgaaa
    55921 accaataaaa tcatgcaact cttgtttatt cataagatat tacctcactt tcttctataa
    55981 gcataatgtc attaaaaatc gatttcaatt ttttcgattc ttcacgtaat acaggtattc
    56041 ttatggacta gacagcgctt tgggaagatg gattctctga aaaatataaa ggttcacttt
    56101 atgagtggat aaaaaaagca gcaaatactt cgacttcttt aatttaccaa cattagtgaa
    56161 aatcgattca tctggagatt tgtaatttag agtccttgtc ttgattagag gtgtataaag
    56221 ctttttatta gacattttta aattccatta caatttgctt ataaactagg tttttaaagg
    56281 tgactatggt aagataatag agatattatt ttgtgattag gagtgtatat atgaataaaa
    56341 atggagaatg gagtgctgca cgacggatat tcattatatt aatggatgtt attgtataca
    56401 atgcagcaat atatagtagt ttcttactga agtttcgtgg agagatccca ttacgaaatt
    56461 tagaaacttt ccaacactca gcaattatta tttcagtgat ttttattgcg ttgaacattt
    56521 tgttgggtgc atatgttttt tataatcgaa tgatcagtga catcgtgttt gtcacagtca
    56581 tcggtcaggt attgatgaca ctagggatca tggtagtgac ttttgcgggc cgctggtttg
    56641 cttttccacg tcttgttatc ttactttcat ttgttctcgg tacgcttttc ttaagtatct
    56701 atcgtattct cgtatataaa ctttatttac gcgtaagtcg agataaaaag gtcgtcatta
    56761 ttgggttaga aaaggatgtc gcaccagcaa tccaaaattt tggttcaaag aaaaacaaca
    56821 aacataaagt cgaagcagtc attcttgata actattatgc aaacatcaaa aatatgatta
    56881 aagacatcga tattgtctat cttgcttctc atatcgaaga aactgaaaaa atcaaaatct
    56941 atcaattagt tactaaaaat gaaaaaaaat tgtttttgaa tacaacattt gaaaacttga
    57001 cattgatcaa tccaaatatt atgaatttcg aagatgaaag tattattgaa gcatctggat
    57061 ttagaatcca acctgaatat gaactattca aacgtttgtt tgatatcatc gtttcacttg
    57121 ttttattagt tattgcttca ccatttatgt tattcacagc aattttagtc aaagtgacat
    57181 caccgggacc aatcatctat aaacaagtgc ggatcacaaa aaatcaaaag gaattttcaa
    57241 tctataaatt ccgctcgatg accgccacag ccgaagcaaa atctggacca gttttagcta
    57301 agagcaatga tgcacgagta acgcctgtag gtaaatttat tcgagctgtc cgctttgatg
    57361 aattaccaca aatcttcaat gtgctgaaag gcgatatgtc gatcgtagga ccacgtcctg
    57421 aacgttcttt cttcgtcgat cagttcaatg cagaaaaccc ttattactac ttacggcata
    57481 atgttcgtgc tggtatcact gggtatgcac aagtctatgg aaaatatgtc tctgattaca
    57541 acagtaaatt acgttttgat ttactttata tcaaaaaata ttcattgatg cttgatttaa
    57601 agatcttgct gcaaaccatc aagatcctat ttgataaaat gtcctctcaa ggacttgaag
    57661 aagacgaaaa tgtgatctca ttggcagaaa ttgcttttcc gaatgaaaaa atctttcgct
    57721 agttgggagg cgtaaaaatg aacaatccga aattcagcat aatcgtacca gtatacaatg
    57781 cagaagcaac tattggtcga acgattgata ttctaaaaga attggattat gaagagtacg
    57841 aagtcgtttt agtaaatgat ggatcaacag atcaaacaca ggaaatcatt caagaactga
    57901 ttgaaaatga tgaacgtttt gaattgatta ctacgaccaa tcaagggccc ggattagcaa
    57961 gaaacgaagg aataagacag tcaaaaggag aatatctctt attcttcgat gcagatgaca
    58021 cgccagtaaa aacgatcttg tcagattatg atcatttgct agaaaagagt ccaaaagcgg
    58081 atcttgttgt tagttcgttt gtttttcgta caatggacga gggaagaatc gtttccgaaa
    58141 aagaaaactt agttacagaa tgtcactatc taactcacga agagtttata aaagacatgt
    58201 atcagttaat gaatgaacag ttgatgtatg ttgtatggaa caaatgctat cgtagcgata
    58261 ttctgaaaaa taaccaaatt ctatttaaaa cttataatag ttgtgaggat cgaatattta
    58321 acttacatta ttatgaatac tgtcatcaag tgatcatgaa cccaaaagta gaatatattt
    58381 atgaatttga aggtgggaag gggatcacta atcaataccg acctaacaag ttcgaaacat
    58441 ttaaagaatt ctattcatta gcaaatactg taacagacga tattaataaa gacggtatgg
    58501 cggcgcttct tttaaaaggg acaacttcag ttattttttc tatttatagt acctctgcaa
    58561 gaacttcaaa agagaagaaa gacgaagcaa agcagatatt gagtgatcca gtgatccgag
    58621 aagccaagca aattgctaag acggattctt cagcaaaaaa aataacaaag ttgctttaca
    58681 atatgccagc tccactattt ttatctgctg taaaagttgg aagcttcgtt gaagtgaaaa
    58741 tgccagggtt aatggctgct ttgaagcgag tatattagtt aatggaggaa aatataatga
    58801 aaagaatttt gcattttcag ggacgaatgg gtttaggtgg agcagagtct ttcatgatga
    58861 atttatatcg taaaattgat cgtacaaact atcaatttga ttttttgatt tatgaagatt
    58921 atgcagatgt tcaagactat cattcagaga ttgaaagatt aggaggccgt atttttgttg
    58981 tacctaatcc taagaaaaat atttttaaat atttaataga agtgaataag ttactaaaaa
    59041 aagaatcttt ttctatagtt cacaatcagg tttattttgg cgggggaatt aatttatggt
    59101 tagccaaaaa gaatggaatc agacagagaa ttgctcatag ccatgcaact gaagatggaa
    59161 aatctcaaaa tattgtaatg aatgtattgc gtaaattttt aaccaaattg ttgttacaaa
    59221 atgcaacaga ctaccttgcc gttagtcaag aagctggtga aagtctgttt caaaaccatc
    59281 cctttgagat agttcataat ggtattgatt tagagcttta tagtaaaaat tcagaagcta
    59341 aggttaataa gagaaaagaa ttggatatct caatgagtac ttttgttgtg ggaaacattg
    59401 gacgattaga gaaacaaaaa aatcaatctt atttgttaga gattttttta gaaatactaa
    59461 aaatgaattc tgattcttac ttgattttag taggtgaagg gtcacttaga aataaactga
    59521 aagccaaagc agtagaattg gatatagaga aaagagtaaa atttttagga gaaagaaacg
    59581 atattcccga gttattatct gtgatggatg tttttgtaat gccttctctt tatgaaggtc
    59641 tgccaatggt tggattagaa gcacaagcag caaaattaaa attagtctta gcagatacta
    59701 tttccaaaga tacaaagtta acaaataatg tggattttgt atcattagat gaaacaccac
    59761 aagaatgggc aaaaaaagta ataaaagagc cgtatgctaa taaaataaca caagaactaa
    59821 agagttatga tgtttcgtac actactgaac aaatggagag tatatatagt agagatcatt
    59881 aaaaatgttc aagatatttt aggagtgatt atttttgaaa atatgttatt tgatattggc
    59941 atacaatgat attggcaact taaagagatt ggtcaatcga ttgaacgaaa atgcagtttt
    60001 tattattcat atagataaaa aaactgatat agaaccattt attgaagcat ttaaatcata
    60061 tgataatgtt gaatttttat gtaataaaaa ccgtgttaaa atttattggg gtggtttttc
    60121 cattgtacaa gcagaaatca atttagtaaa gagagcatta caaaatgaga aatatttaaa
    60181 atatgtatta ttatctggtg ctgattatcc aatcaaagat aatgagtata tttataatta
    60241 tttt
//
LOCUS       DARBDV010000006        54772 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000006 DARBDV010000000
VERSION     DARBDV010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.006"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..1589)
                     /locus_tag="SCM56_000387"
     CDS             complement(<1..1589)
                     /locus_tag="SCM56_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEG1109216.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKI"
     gene            complement(1604..5125)
                     /gene="mfd"
                     /locus_tag="SCM56_000388"
     CDS             complement(1604..5125)
                     /gene="mfd"
                     /locus_tag="SCM56_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEG1109217.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(5142..5702)
                     /locus_tag="SCM56_000389"
     CDS             complement(5142..5702)
                     /locus_tag="SCM56_000389"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEG1109218.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            6055..7029
                     /locus_tag="SCM56_000390"
     CDS             6055..7029
                     /locus_tag="SCM56_000390"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEG1109219.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            7199..8599
                     /locus_tag="SCM56_000391"
     CDS             7199..8599
                     /locus_tag="SCM56_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HEG1109220.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(8677..9399)
                     /locus_tag="SCM56_000392"
     CDS             complement(8677..9399)
                     /locus_tag="SCM56_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1109221.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(9478..9984)
                     /locus_tag="SCM56_000393"
     CDS             complement(9478..9984)
                     /locus_tag="SCM56_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEG1109222.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            10107..11906
                     /locus_tag="SCM56_000394"
     CDS             10107..11906
                     /locus_tag="SCM56_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEG1109223.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(11974..13548)
                     /locus_tag="SCM56_000395"
     CDS             complement(11974..13548)
                     /locus_tag="SCM56_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEG1109224.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(13803..14312)
                     /gene="mreD"
                     /locus_tag="SCM56_000396"
     CDS             complement(13803..14312)
                     /gene="mreD"
                     /locus_tag="SCM56_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEG1109225.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(14316..15170)
                     /gene="mreC"
                     /locus_tag="SCM56_000397"
     CDS             complement(14316..15170)
                     /gene="mreC"
                     /locus_tag="SCM56_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEG1109226.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(15325..15963)
                     /locus_tag="SCM56_000398"
     CDS             complement(15325..15963)
                     /locus_tag="SCM56_000398"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEG1109227.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(15964..16614)
                     /locus_tag="SCM56_000399"
     CDS             complement(15964..16614)
                     /locus_tag="SCM56_000399"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEG1109228.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(16627..17526)
                     /gene="rsgA"
                     /locus_tag="SCM56_000400"
     CDS             complement(16627..17526)
                     /gene="rsgA"
                     /locus_tag="SCM56_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEG1109229.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(17691..19760)
                     /gene="pknB"
                     /locus_tag="SCM56_000401"
     CDS             complement(17691..19760)
                     /gene="pknB"
                     /locus_tag="SCM56_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEG1109230.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(19757..20497)
                     /locus_tag="SCM56_000402"
     CDS             complement(19757..20497)
                     /locus_tag="SCM56_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEG1109231.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(20510..21868)
                     /gene="rsmB"
                     /locus_tag="SCM56_000403"
     CDS             complement(20510..21868)
                     /gene="rsmB"
                     /locus_tag="SCM56_000403"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEG1109232.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(21868..22806)
                     /locus_tag="SCM56_000404"
     CDS             complement(21868..22806)
                     /locus_tag="SCM56_000404"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEG1109233.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(22799..23290)
                     /gene="def"
                     /locus_tag="SCM56_000405"
     CDS             complement(22799..23290)
                     /gene="def"
                     /locus_tag="SCM56_000405"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEG1109234.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(23305..25713)
                     /gene="priA"
                     /locus_tag="SCM56_000406"
     CDS             complement(23305..25713)
                     /gene="priA"
                     /locus_tag="SCM56_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEG1109235.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(25966..26271)
                     /locus_tag="SCM56_000407"
     CDS             complement(25966..26271)
                     /locus_tag="SCM56_000407"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEG1109236.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(26276..26890)
                     /gene="gmk"
                     /locus_tag="SCM56_000408"
     CDS             complement(26276..26890)
                     /gene="gmk"
                     /locus_tag="SCM56_000408"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEG1109237.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(27377..27661)
                     /locus_tag="SCM56_000409"
     CDS             complement(27377..27661)
                     /locus_tag="SCM56_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109238.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(27679..28569)
                     /locus_tag="SCM56_000410"
     CDS             complement(27679..28569)
                     /locus_tag="SCM56_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HEG1109239.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(28589..28807)
                     /locus_tag="SCM56_000411"
     CDS             complement(28589..28807)
                     /locus_tag="SCM56_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEG1109240.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(28777..30477)
                     /locus_tag="SCM56_000412"
     CDS             complement(28777..30477)
                     /locus_tag="SCM56_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEG1109241.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            30744..31517
                     /locus_tag="SCM56_000413"
     CDS             30744..31517
                     /locus_tag="SCM56_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109242.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(31598..32167)
                     /gene="thiT"
                     /locus_tag="SCM56_000414"
     CDS             complement(31598..32167)
                     /gene="thiT"
                     /locus_tag="SCM56_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEG1109243.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(32233..32335)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(32341..33312)
                     /locus_tag="SCM56_000415"
     CDS             complement(32341..33312)
                     /locus_tag="SCM56_000415"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEG1109244.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(33406..34779)
                     /gene="glmU"
                     /locus_tag="SCM56_000416"
     CDS             complement(33406..34779)
                     /gene="glmU"
                     /locus_tag="SCM56_000416"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEG1109245.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(35282..36100)
                     /gene="purR"
                     /locus_tag="SCM56_000417"
     CDS             complement(35282..36100)
                     /gene="purR"
                     /locus_tag="SCM56_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEG1109246.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(36267..37091)
                     /locus_tag="SCM56_000418"
     CDS             complement(36267..37091)
                     /locus_tag="SCM56_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEG1109247.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(37064..37744)
                     /locus_tag="SCM56_000419"
     CDS             complement(37064..37744)
                     /locus_tag="SCM56_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEG1109248.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(37767..38705)
                     /locus_tag="SCM56_000420"
     CDS             complement(37767..38705)
                     /locus_tag="SCM56_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEG1109249.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(38950..39813)
                     /locus_tag="SCM56_000421"
     CDS             complement(38950..39813)
                     /locus_tag="SCM56_000421"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEG1109250.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(39955..42528)
                     /gene="mprF"
                     /locus_tag="SCM56_000422"
     CDS             complement(39955..42528)
                     /gene="mprF"
                     /locus_tag="SCM56_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEG1109251.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            42669..43475
                     /gene="proC"
                     /locus_tag="SCM56_000423"
     CDS             42669..43475
                     /gene="proC"
                     /locus_tag="SCM56_000423"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEG1109252.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(43554..45110)
                     /gene="rny"
                     /locus_tag="SCM56_000424"
     CDS             complement(43554..45110)
                     /gene="rny"
                     /locus_tag="SCM56_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEG1109253.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(45521..46570)
                     /gene="recA"
                     /locus_tag="SCM56_000425"
     CDS             complement(45521..46570)
                     /gene="recA"
                     /locus_tag="SCM56_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEG1109254.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(46679..47920)
                     /locus_tag="SCM56_000426"
     CDS             complement(46679..47920)
                     /locus_tag="SCM56_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEG1109255.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(48160..48738)
                     /gene="pgsA"
                     /locus_tag="SCM56_000427"
     CDS             complement(48160..48738)
                     /gene="pgsA"
                     /locus_tag="SCM56_000427"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEG1109256.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(48947..49870)
                     /locus_tag="SCM56_000428"
     CDS             complement(48947..49870)
                     /locus_tag="SCM56_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1109257.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(50609..51895)
                     /locus_tag="SCM56_000429"
     CDS             complement(50609..51895)
                     /locus_tag="SCM56_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEG1109258.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(51888..53156)
                     /locus_tag="SCM56_000430"
     CDS             complement(51888..53156)
                     /locus_tag="SCM56_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEG1109259.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            53408..53869
                     /gene="mscL"
                     /locus_tag="SCM56_000431"
     CDS             53408..53869
                     /gene="mscL"
                     /locus_tag="SCM56_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEG1109260.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(53929..54375)
                     /locus_tag="SCM56_000432"
     CDS             complement(53929..54375)
                     /locus_tag="SCM56_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1109261.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(54468..54713)
                     /locus_tag="SCM56_000433"
     CDS             complement(54468..54713)
                     /locus_tag="SCM56_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HEG1109262.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
BASE COUNT        15369 a        11836 c         9856 g        17711 t
ORIGIN      
        1 ttaatttttt tatttaatcg caggattttc ttaccaaaag gcaacattag ccattctcgt
       61 actgtaaaca gacgaagacg aatcactgca taaatgaaca cagaagcccc gatcccaact
      121 cctatcacgc tgacaaaaaa agcagtggaa cgatgagtta caggaccaaa tactatattc
      181 aatacaccat aatacagcag cagtactact gtcatacaaa gaaggctgaa catcaacttc
      241 cgtccaaaat gccgttcttt ccaaaaagca tttagctctt ttgtttcaat tcgaacaaaa
      301 taccaaagcg tcacacctaa tcctaaaagg gtagacaggc ttgcacctgc tgttcctaat
      361 aaaccggtca gaaaagaagt agtcaatcct tttaccaata gtccccatcc tgcgcctttt
      421 aaagaagggc gaaacgagtt cttgctttga gcaatacttt gataagcttg gatgactgca
      481 gtgaaagcaa tggaaaaaac aaataataca agcgctgcat ttcccgcata atctttaaac
      541 aacgcataat tgatataagg gagcagcaag gctaatccca aagacgcagc taatgctaaa
      601 gcggtcgtca atctaagata gattttggct gtctgtaaaa actgacgata gatccggttg
      661 gtcaaatgac gagtcaacgc aggcaggaat gtcgcactta atgcagtggc aatcactaaa
      721 cctaattgta ctaaaggctg acctcgatca tagacccctt ttgctatctt cgcaccgtat
      781 tctgacagtc cgtaaacttc caaagcattt tttacaaaga aagagtcgat cagctggaaa
      841 aagataagca atccactata aatcgaaacc agtccgcctt ctaccagaaa acgccggatc
      901 aagggctttg acggctgttt gaacaatgga aaccgtcgga agcttaacgc tcctccatga
      961 atcttttgtt catagtagtg aaggatccca tatgcgcata aaccgccaaa aacagctcca
     1021 ctcattgctg ccgtccctgt ttggtacacc gtccatccaa gctttctaaa agcaaacgca
     1081 ctacagacaa tcacaaaaac acgaacgaac tgctcaacta cttgagaaac agcagttggt
     1141 tccatcaaga atcttccttg gaaattccct cgataaaaag atagaccagg catcagcaaa
     1201 aaggtaaagg aaactacttg gatcagcggt ttcaattgta tgtctcccat catccaagca
     1261 atccagctgc taaatgcaaa gacaaacaac cacaaaaaga cacctgtcca aaatactaag
     1321 ggataaagat tttgcaaagc ttgctttcgc tcgaaaggat tctttctttc tgcaacgtac
     1381 tttgatatga actgcggaag accggataaa gctaaagtca ttgctatccc ataaattgga
     1441 taaacttgct gataaacata aaaaccttcg tcgccgacta agttttggag gggaattcgg
     1501 taaagggcac ttaatacttt tgctatgaaa gaagcaatcg tcaatacaaa tgctccttgc
     1561 atgacgcgcc gcatttcttt atgatccaaa tcattgctcc ttcctacgct gacagatatt
     1621 tttgctcacg caacgctttg acaaactttt ggatttcctg aagccatact gcttcttcca
     1681 tttggttggg gatcgtcaag cgtatcacca tctgttcttt ttcaacgcct agactggctt
     1741 ttagttttgt cgctgtcagt gcttcgaata actgttccac actatagcgt tttgttccaa
     1801 ctttacttag agtaaagact atatgcgcct cctgtttcct tatcgtttct aggagcgctc
     1861 gatcgccatc cattttgatc tgcccaattg tcaagagatg tgctacttct tctggatatt
     1921 ctccaaaacg gtcgagcaga tcatcttcta gttcatctag catttcttgc gaatcaagtt
     1981 cacgaattcg tttatagatc tcgattttct gccgttgatc tgccacgtat gtttcaggta
     2041 gatacgcatc gacaccgagg tcgatctcaa cagtggtctt ctccacttga ctattctttc
     2101 cttgtttacg agaaacagct tcgcttagca tttgagaata catatcgaaa ccgactgcat
     2161 caataaatcc atgctgctga gcacctaaga gattacctgc accgcgaatg gaaagatccc
     2221 gcatcgcaat cttgaatcca gatcccagtt ctgtaaaatc tttgattgcc tgaagacgtt
     2281 tttcactgac ttcgttaagg atcttctgct gctcatacat aaagtaagca tatgcgacac
     2341 ggctgcttct tcctacccgc cctcgcaatt ggtataacgt ggataatccc atataatcag
     2401 cgttctcaac gaacaacgta tttgcgttcg ggatatctac tcctgtttca atgatggttg
     2461 tagtgacaag aacatcatat tgaccttcaa taaattcaaa aagcgtattt tctaattgcg
     2521 cctctgtcat ttgcccatga gcataaccga tccgtgcttc cggaacgagt tcttggattt
     2581 cttctacttt ttgttcgatc gtttctaccc gattatataa atagaatact tgtccacctc
     2641 gtcccatttc tcgatggatt gcttcgcgaa tagccccagg atttttttcc ataacatacg
     2701 tttggatcgg ataacgattc gcaggcggtg tttcgattac tgaaagatcc cgcacgccta
     2761 acatcgacat atgcaaagtt ctcggaatcg gtgttgcagt cagagtcaaa acatccactt
     2821 gtgcacgtaa ctgtttcaat cgttccttat gttttacacc aaaacgttgt tcttcgtcaa
     2881 tgatcagtag acccaaatcg ctaaattcaa tatcttttga caaaattcgg tgggttccta
     2941 caacaatatc aacttgacct gtacgcagtt gttcgatggt ttctttttgt tgctttcttg
     3001 ttctgaaacg acttaatagc ccgatattta cggggaaacc ttcaaatctt tccagcatcg
     3061 tttcatagtg ctgctgtgcc aagatcgttg ttggaactaa aaaggctact tgtttgcttt
     3121 cctttatcgc tttaaaggcc gctcgaagcg ctacttctgt tttcccataa ccaacatcac
     3181 ctacgagtag tcgatccatt ggtttttctt tttccatatc ccgtttgatc tccgctgcac
     3241 ttctcaactg gtcatcagtt tcagagtagg ggaaggcatt ttcgaattct ttttggtagg
     3301 cgtcatcagg tccaaaagca tatccttttt ctgactctct tgcggcatat aatttgatta
     3361 gatcgtcagc gatatcctcg atttttgaag agacttttcg cttggttttc gtccattcac
     3421 ttccgcctag cttattgatg cgaggcgctt tggcttcaga ggcgacatat ttttggatca
     3481 gattcagttg agtaacagga ataaacagtt tgtcgtcgtt ttgatacaaa atagtcatat
     3541 agtcttggtg aacgccatct acttctagcg tttccatccc aatgtatttc ccgataccat
     3601 gattggcatg gacaacgtaa tctcctgctt ttaactcatt atagcttttc aatcgctctg
     3661 cgtttgttac tgtttgacgt ctcgtttgtt ttttcgtcgt tttttggaaa atttcttttt
     3721 ctgtgatagc tactattttt tcttgaggaa gttcaaaacc agcctgcaaa gctccttcaa
     3781 ctaattgtac ctgtcctctg atcaatgcat ttttttctgt ttctactact aatatgtctt
     3841 catctcgaag catttgttct gctttgcgta ttctgtcctt tgtcggaata aagatcacaa
     3901 cagtctgttt ttgcttgcgc caacggtcca tttctgtttt cagcaatggc atttgtccga
     3961 agaactgttg catcgtccga tattgaaaat tatggattgc ttggaaacga agatttccca
     4021 tccctttttg gaagagtgag aaaaaggttg tggtgaactt ttccttctgc acgatttttt
     4081 gtatatctgc tgaaaatgtc tgctcagaaa acacccgcaa ttcgctgatt ttttgagtct
     4141 gccattctgc cgcttccctt tcgatttccc gatttgtttc catgattcgc tgataatcat
     4201 caacgactag taaactattt gaagggaaat aatccaagat cgtcacagat tcttggtaaa
     4261 gaagatctga gtagaatttt gcttcttctg tcgggacacc tgcttgccag cttgaagtca
     4321 gctgaccgaa gtaatcttgc aaaaactcgc ggtctgcttc ttctgtggtc gcagcaagcc
     4381 gtttttctag cattttttgc atttgctcta ttccatgttc tagatcctct ggtccgtaga
     4441 cttgttcact cgttggggaa acccagattt cttccgtttt ttcgattgag cgttgtgttt
     4501 ctgcttcaaa ataccgcata gaatctatct ctacatcaaa tagttctaca cgaacaggat
     4561 aggttgtcgt taacgggtaa atatcgataa tgcttcctcg aatactgaat tctcccggtt
     4621 ttgcgaccat ctcttctcgt acatagccca tcaaagtcaa ttggcgagga agattttcca
     4681 cgtctatctc atcaccaata tgccaattca attgcgcctg tagccaagtt ttcttattcg
     4741 gcagtcgttt gtgcaaagat gcgactggca acaaatagat acctgctttt ttttcttgaa
     4801 gtgcgttaag cgttgcaact ctctcgcctc gcgcttctgg agaagaaaag gccatttcag
     4861 cagaaagcac ttcatccact ggaaataaat ggatctgatc ttgcggaatc acatgttgca
     4921 gatcttctgc taactgattc gcataataca aatttggcat cagaataatc atgtttttat
     4981 ttgttttctt aaacgctcga gcaaatagaa gtgttttcgc tgagcccgct aatcctgtga
     5041 ttagttgtct aggcattttt tcttctaaat tgctgaccca ttcttttact tgcggatctt
     5101 ttccgactaa ttgaataata tccattcctt ttcctccttc ctcaattgaa tcgattcatc
     5161 gcatccacga acgaatgccc ttctatgaga tattcgactg ctttaacaga ttgacacatg
     5221 ctttcttcta tcaaaggctg attttctttg ctgaaaggac taagcacatg ctggactact
     5281 gtttttttcc cttctggcct gccgattccc accttgattc gatcaaagac attcgtattc
     5341 aaatgggaaa taatactttt gataccgtta tgcccgccgg cacttccctt ttgacggagc
     5401 cggatttttc ccactgccag atccaaatca tcgtagatta cgactaattc ctctggataa
     5461 atcccaaaat aagtcattag tgggccgact gcacgaccag attcattcat gaatgtttgt
     5521 ggtttgacta aaaggatctt ttctccatta tgaaaaaatt cggcaacttc tgcttcgaac
     5581 ggattttttt tgaaagtagc ttgatgctcg cgtgcaattt tatccacgac cataaagccg
     5641 atattgtgtt ttgtatattg gtatttggta cctgggtttc ctaacccgac gatcattttc
     5701 atttttgttt tccacctaat tctaaaaagc agcacaaaag gctggactgc taattagcgg
     5761 tccagtccat cgagcttttt tcttttttac acttgtcaat gcatagtata acacaaaaca
     5821 aggcgtttcg gctaaaagag aatcgttaat aaatgataaa gaaattaaaa atttcttcaa
     5881 agtttaatga aataattgat atataaaaat agtgaaataa cgcacatgta agcttttaca
     5941 tataatacaa ccttgttatc tgttatattt tatagccaaa tagcgtgaca tggggcttga
     6001 gaaatgttag cataagcatg ggaaaaaaca aatcaatgga aggattgata ctaaatgact
     6061 gcagctgcag aaaaaaaaga tcatcaaaaa gttattcttg ttggagacgg cgccgtaggt
     6121 tctagctatg cctttgctct agttactcaa aatatcgctc aagaagtagg tattgtggat
     6181 atcaatactg ctaaaactga aggagacgca attgacttgt ctcacgcttt ggcatttact
     6241 tcacctaaaa aaatctattc tgctacttac gcagacgcac atgatgctga cttagttgtt
     6301 attacagctg gcgcaccaca aaaaccaggt gaaactcgtt tagacttagt aaacaaaaac
     6361 ctaaaaatca accgcgatgt tgtcacacaa atcgttgaat caggatttaa cggtatcttt
     6421 ttagttgccg ctaacccagt agatattttg acttactcaa cttggaaatt ctcaggtttc
     6481 cctaaagaac gcgtaatcgg ttcaggtacc tctcttgact cagctcgttt ccgtcaagca
     6541 cttgcggaat tagttgacgt tgatgcacgt aacgtccatg cttacatctt aggtgaacac
     6601 ggagattcag aattcccagt ttggtcacat gcaaatgttg ccggcttgca aatctatgaa
     6661 tgggtaaaaa acaacccaga aatcgatgaa gaagctatgg ttaacctatt ctttggcgta
     6721 cgtgacgctg cttacacaat tatcgagaaa aaaggcgcta ctttctatgg tatcgcagta
     6781 gcattagcac gtatcactcg tgccatctta gatgatgaaa atgctgttct tcctttatca
     6841 gtattcatga atggcgaata tggattgaac gacatctaca ttggtgcacc tgctgttatc
     6901 aaccgtcaag gtatccaaaa agtaatcgaa attcctttaa gtgacagcga gcaagaccgt
     6961 atggctgctt ctgctaagca attaaaagaa attctagatg aagcttttgc taaactagac
     7021 gctgaataat ctttgaacat gacggaaaac atctattcgg tgttttccgt ttttttattt
     7081 tatcaccctt atggtttcta aattttttca taaagtagag ataaccttcg tcttttcctc
     7141 taaattatgc tatcatgaga ccgttccacc ctaaactttt acttaaggag ttgtcgatat
     7201 gacaagatct tcgcatcgaa aaaaatcgaa acatgttgct ttgctttcag ttcttggtgt
     7261 tctcttagtg ctgatcagct tttataccta tcgcagtaca tactacacaa agcatttttt
     7321 gccgaataca gaaataaatg gaataaatgt cagcaatcta acagtcgaaa aagcaaatga
     7381 aaaattaaaa gaagcttatt ctgataaaaa actggctatt aaagaagatg gaaaagtctg
     7441 gcaggaagta ccaaaatccg agctcggcta caaagatgat ttcacttcgg agctttctcc
     7501 gatcctaaat gaacaaaacg gatggacttg gggaatgact tatgtctcag cggcagaaaa
     7561 acaagaaata gatcctgtca gtcaaaacca ccaaaaacta gacactaccg ttcagacatt
     7621 gacaactaag ctgactgatt tgaacaaaga ccggacacct actactgatg caacgatcga
     7681 aaaagcaggt gactctttcg ttatcaagcc tgaagtcaat ggaaatacga tcgacgtcga
     7741 tgctgcggtc aaacaattaa aaagtgcggt aaactctggt aaagatacaa ttgaattgac
     7801 tgaattcaaa gaaaaaccaa aagtaacatc tgaagacagc tcactgaaag aacaactcgc
     7861 atccatgaat gctatcgcaa atgtaaaagc tacttacagt ataaacggtg agactttcca
     7921 gatcccatca agtgatatta tgagttggct gacctacaat gatggaaaag tcgatttaga
     7981 tacagaacaa gtacgtcaat atgtaacaga tttaggcaca aagtataaca caagtacgaa
     8041 tgacacaaaa tttaaaagta caaaaagagg agaagttacg gtcccagttg gcacttacag
     8101 ttggacgatc caaacagaca gtgagacaga agcattgaaa aaagcgattc tggcaggcca
     8161 agattttact cgttcaccca ttgtgcaagg tggcacaacc gctgatcatc cattgatcga
     8221 agatacgtat atcgaagttg atctggagaa ccagcatatg tggtattaca aagacggaaa
     8281 agtggccctt gaaacagata tcgtttccgg aaagccaact acacctacac ctgctggagt
     8341 cttctatgtt tggaataaag aagaagatgc aactttaaaa ggaacaaatg acgatggaac
     8401 accttacgag agtccggtaa actactggat gccgattgac tggacaggtg ttggtatcca
     8461 cgattcagac tggcaacctg aatacggtgg agacttatgg aaaacccgcg gttcacacgg
     8521 ctgcatcaac accccaccaa gtgtaatgaa ggaattattc gggatggtcg aaaaaggaac
     8581 gcctgtattg gtcttctaac aaaaataata tataaaaaga aagcacccgt acgattggta
     8641 caggtgcttt ctttttatta tctaacaatg tgctacttac atttcagcag cagtaaagac
     8701 ttctgaaaca tcatcatcgt cttctagctt atctactaga cgttccaatt gctctttttg
     8761 ttcctcattt aaatcaatcg tcgtttgtgg aatcatcgtc aattctgctt gcgccaaagt
     8821 gaactctttt tccaactcat ctcgtacagc agcaaaatct tctggtgcag tataaatctc
     8881 gaatacttca ggagaagttt gcagatcttc tgcccccgcc tctaatacat cctcaaacat
     8941 cgtctcttca tcaacagtca acccatcgcg ttcaatagcg atatatcctt tgcggtcaaa
     9001 caagtaactg actgaacccg tttctcccag tgatccaccg tttcgagtaa aagctactcg
     9061 gacatttgtc gctgtccgat tacggttatc tgtcaatgca tggactaaga ttcctactcc
     9121 acccggacca tatccttcat acgtgatttc atcgtaattc gtttcatcta cggcactcgt
     9181 agccttttta atcgcccgtt ccacattgtc gttaggcata tttgcagctt ttgctttgtc
     9241 cacaacaagc cgcaaagctg gattcataga tggatcagga ccgcctgctt tagctgccat
     9301 ataaatttca cgagaaagtt tctgaaagac tttccctcgt tttgcatctt gggcattttt
     9361 tcttccttgg atattatgcc atttactatg tcctgacatg atttttcctc ccttgttctt
     9421 ttgagaatac acttctattc tagtactatt tcacaaaaaa acaaagccac agccaatcta
     9481 taaacgcttc ttgaatgccg caaaaagcac acataatatc acagcagtca gagcaccgat
     9541 cgaatccaac atgacatctt ggaacatagg tgttcgtcca ccggtcatca tctgatggaa
     9601 ttcgtccaaa cctgcgtacc ctgttgctga aagccaagca aggatcgctg tcagccacca
     9661 aatttttagc ttggggaaaa cacctaaaaa caagctgcct cccaaaataa aataggtacc
     9721 gaaatgtgcg cccttcctta aaaagaactc taaaaatttg gcgtagccca gatggttgat
     9781 actcaccata cttccaccat aattaaaacg tatacccgct aatgtgtctt taaatggctg
     9841 attaggcagc cagctttcta atcgcgaaac ttgtgattgc tgtccgtaag tttgagaaga
     9901 actgtaaaat aagattataa cgactatcag tgcgattact aaataaatat ttccgttttt
     9961 catttgtttt tttgaagaat tcataagtca cctctgtatt ttatcttaga ctttttttga
    10021 agaaacaaca atcattttca tagagacttc ttccataaaa aagtgctata cttaattttg
    10081 tattacaata tgaagggagt tttttcatga gttattcatt aaattggaat ctagattcta
    10141 ttttttccgg aggcagtcat tctgatgctt tgaaccaacg gatgaagcaa ttggaagacc
    10201 aaatgaacga gtattatcac cgcgtaacaa aatggtcacc tagttctgac aatgcagagc
    10261 agttaaacgc tattcttcaa ttgcaagaaa cgattacgaa tggttttacc caatgcagca
    10321 gctatattac tgcattgctt tctgccaatg tcaatgattc agatgcaaag gtcttatcag
    10381 gcaagcttta cgcaatgctg cctcgtctgc aatcagccga aactgtattg tcaaagaaat
    10441 ttgcagaaat atctgacaat gattggaatc agatgctttc tcaatcttcg tttgaaacaa
    10501 ttgcttttcg tttaaacgag attcgtcgtg atggctcaca attattatca gaagctgaag
    10561 aaaacattat aaatacgctt tctttagatg gtctaaatgc ttggagcagt cattatgata
    10621 cgattgtagc cagcatttcg attcccttcg aacaagatgg acaagtagtc gaattatctg
    10681 ctggacaagc cttcaataag atgatgggcg atccagatcc aaaagtcaga gaaacacttt
    10741 tcgcagcatg ggaagaagcc tggaaagaaa aggcacccct cttcacggat actttgaatc
    10801 atttagatgg ttttagactt tctgactatg aacttcatgg tgtcacagat tttctacaaa
    10861 aaccattaga gtacaatcgg ttgaagaaag aaacattgtc tgtcatgtgg gatacaatcc
    10921 agaaaaacaa acaacccctt gttaattact tgactagaaa agctaatctt ttcggtaaag
    10981 aaaaaatgga atggcaagat caagatgcac ctattatttt aggcgatttg aaagaaaaaa
    11041 cctttagttt tgacgaagca gcagcattta ttatcgaaaa ctttaacaaa ttcagtccaa
    11101 aaatggctaa atttgcccaa tctgcttttg aaaagagctg gatcgaagca gaagatcgtc
    11161 ctgggaaacg tcctggtggt tattgtactg agttaccaga aacaaaagaa tcaaggatct
    11221 ttatgaccta cagtaattct gttaatgagg ttgcgacact tgctcatgaa ctaggacatg
    11281 ctttccatag cagcacgatg tgggatttac cttctttaaa tcgcgaatat gccatgaatg
    11341 tcgcagaaac agcaagtaca ttcgcagaac tgatcgtagc cgatgctaca ttaaaagtcg
    11401 caaaaacaaa agaagaaaaa atcaatttgc tagatacaaa actgcaaaat gctttagcta
    11461 tgttcatgaa tatccattcc cgattcattt ttgaaaatcg attctatgaa gcacggcaag
    11521 aaggattagt ctcagaagat aagatcacag agatgatggt tgaggcgcaa aaagaaggct
    11581 atcaaggagc tttagctact taccatccat atttctgggc tgcaaaattg catttcttta
    11641 ttgatgacgt accgttttat aacttcccat acacatttgg ctacctcttt agtttgggca
    11701 tctatgctta cgccaaccaa aagggagcaa attttgaaga agaatatatc catttactac
    11761 gcgatactgc ctcaatgacg acggaagatc ttgcgaagaa acatttgaat gtcgatctta
    11821 cccaaccaga tttttggcaa gcaggtatcg acatggtgac tgaagatatc caaacgttca
    11881 tggaattgac agaagaattt atctgatttc tattttatca caataaaaaa agagttagtc
    11941 cagaatgggc taactctttt tgattaatca ttcttacatg ctgacagcaa agtcaggggc
    12001 aaaccattga actgaaccaa cttttacgct ttcgcctggt tgaggagcat ggatatattg
    12061 tcctccgcct aaagcaatcg ctacgtggta agttccgcct tgtgaccccc agaataacaa
    12121 gtctccagct tttgcttgag aaactgaaat ttttgttcca gctgattctt gaggaactgt
    12181 ccaaccaccg atgtcacgac cagttacttg catgtaaaca tagcgtgtga accctgagca
    12241 gtcaaagcca cttggatctt taccgcccca aacatatgga gtaccaatat atttgtaagc
    12301 ttctgctacg attgctgcac cattcacact tccagaagga gctggagcag gtgttacagg
    12361 ttttggttgt tcaggtgttg gtgttggcgt tggagccgga gtactacttc ctgtgccgtt
    12421 tcctgtatca acactttgat ctgttgatgg tgttgtaggc gcaggtgttg ttgattcttc
    12481 tgttgttgaa gattcaggca ctgttgttga ttcttctgtt gctgaagatt caggcactgt
    12541 tgttgattct tctgttgctg aagattcagg cgctgttgtt gattcttctg ttgctgaaga
    12601 ttcaggcgct gttgattctt ctgttgaaga ttcaggtgtt gttgattctt ctgttgctga
    12661 agattgtgta gctgatgatg attcttcagt cattgaagat tgtgtagctg atgattcttc
    12721 tgttgctgag ctttgtgcag tagatgaagc ttgagtagct tgtgcttcag cctctgcttg
    12781 ttcgcgtgct tctttttcag ctttttcttg tgcagcttgt tgacgagctt gttctgctag
    12841 acgttgttgt tcacgaatac gagcttgctc tgcttcagct tctgcttttt gacggttcaa
    12901 gtcagctttc ttgtcttcag cagttgcttg ttcagcagct aatgaagttt tcagtacgtt
    12961 caaatcagct tgtttagata aaagatcgcc tttttgagat tccaaagcag cttggttttc
    13021 agctaattct tttagtttag catcgttttc agcttttttg tcttctacag cttttttatc
    13081 ttgtttttgt tgttccatca agtcgttgtt tgcttttacc atcgttgtca ttgcttgaac
    13141 gcgtccaata gcatctgcta atgaatcagc attcaataca gcatcgatgt agtttgagct
    13201 tgtgttgctt acttgtgctt cgcgtgcttg tttttggatt gtatcttcac gtttttcaat
    13261 acgttcttgt aaatctgcaa tgtcttttac taattgtgca gattcttgac gcaatgtatc
    13321 ttgttttgct aataggtctt gtgcttgtgt gttgatttca gaaacttggc tttctaaggc
    13381 atcgatttgt gattgtgcat ctgcttgttg atttttcaag tctgcaatct tttgatcttg
    13441 ctgttgtatc tgagaatcaa aatcgtccgc cgcggcagcg atcggtgatg ctacggcagt
    13501 caaggtcatt gaacatacca ttactgctga tattaaactc tttttcactc ttcattcctc
    13561 cgactggctt aatttaataa atatctaaat atatttaagt ctttcttaac gatataaaaa
    13621 ttgtaccata gcagtgtgtc aacgatataa tagttatgtt acaaaaatgt ttcaaaacaa
    13681 cttttaaaaa tgacaaaact gttcgtttca gaaaatgatc atgaaaaagc cgcgttaagt
    13741 ctagcatttc ttaagataac aacgattctt tttcgctttc gacctaaaaa taaaaaaaga
    13801 aattattcat cactgaataa tttcttaaag ggaaaaatga ataatacaaa tataatcatg
    13861 ttcaacagca gtgtaggtcc taaaaacctt gtaataaaat acgtgttatt tactactgct
    13921 aatttaaaaa tcaactggac caccatcgta aacaattcat aacccgtgac aaagatgatc
    13981 atactgaaaa attcagtaaa gatgttgaca tggataacgt ctttcattac atacatcaac
    14041 catacaatta aagggagagc tactgcatag ataccgataa cgccaatata gtaagcgtca
    14101 tagatagccc cgagaacaat cgtggtaatc agtaaataac gtttttcaaa cgccatactg
    14161 caacataata atgccaatat cagaaagtgg gcatttgaca tataggtgcc tttcgaccac
    14221 tcccctagca ttcttgttaa atgaccatct atcaacatca aaaagaacaa aacgattggc
    14281 agatagtact tcattgtttc tttcttcaac atggactaat cctccgctag ccgttggaca
    14341 atcgtcacaa ctgacacgtc atacatttct gcataaggtt tcacataaac ttcccgatcc
    14401 agcccataac tatctggttt cgttttaata accgtaccga tcggcaagtt agctggagaa
    14461 ttccctccaa gaccggatgt ctggacaaca tccccttctt tgatatccgt atcaccagtt
    14521 aattgggtca ccactaaggc atggagcttt tcatcatagt ttttaagcaa accaaacgct
    14581 tcgccattag ccgaagatac ccgtactgga aaatgattgg agctttcatt agaggatgac
    14641 agtaattcga ttttagacga agccgtattg acctcgatca ctcggccaat caatcctttt
    14701 tgcgacatga ctgccatatt tgcttcgata ccgtctttag aacccttgtc tacgatcagc
    14761 atgtcctgcc aactgtcagg cgaacgagta atgacatttg ccgttacttt ttcgtaacta
    14821 gtcagcgtct gattcaactc aagttctttt ttcaacgctt cgatttctct ctcgtaattc
    14881 ttgttttgct gtgaaacgtc atcatagcta tcgatttttt cttttagccg ctcattttcg
    14941 gcataagttg aaaaaagatt atggacagaa tctactccat ttcgtaccca ccgtactggg
    15001 gcagagattg ttttatcaac cattgcaaca ctgtcattaa cgatggattg gaagaaattg
    15061 ggctttcctt cattcgctcg ctgcgcagcg gtcacactta gtatcgtcac cacgatgata
    15121 acaataacca aggtaataat aatattttta ttaggattga acttcttcac aaagacacct
    15181 cgaattaatt agactcttgt gttcaatttt atcactaaat gccgtgaaac aaaaggtaag
    15241 ttgctcctct taagcttttt tttagcaaat aatagattct gtcattttta gcctgaaaga
    15301 caaatttttt tatttgttta tggatcaatc tttgctttga ataactgcaa tcataccatc
    15361 tgaaaaagaa aattctgcgg tatctgaaag aaattcgtta cttgcataag atgtcggata
    15421 ttctacttct ttattttcca atacatactt gctgcctttt aatgtaagat tcgagacggg
    15481 agtcaagcag caataagcta aatacttcat atctggtatc tgtcttacta tatgctctcc
    15541 aggcaaataa taagacaaat aattttgaca atctctgata ataagctgat gggcaaaggg
    15601 tttgaagcga ggttccatac ctaaccagag attggctaaa agatgatcca atcttccgcc
    15661 cgtagctcca atcaaagtga cttccgcttg cgggtaacgt tcaaatgttt ttaagatacc
    15721 taactgtgta tctgtatcgt ctttttctgc aggggaacgg gtatactctt tggcttttgc
    15781 aaatacttcc ttctgctctt cgttcgacaa tgaatcaaaa tcacccactg ccagatctac
    15841 tgggtagcct gcttgaagca ggtacagcgc accacggtct atcccgacat aggcatcgta
    15901 tttggacagc agagactgcg gccagttttg cggattacct ccagcagcta ataaaatttt
    15961 catttagtct aatgcctctt ttaatcgttc gatttgtccg actggatttt cggctccata
    16021 gatatatgat cctgcaacga agacatccgc ccctgcatct ttacagattt ttgctgtttc
    16081 tggaacgatt ccgccatcta cttcgattgc ataagtccaa ttgttctctc tacgtagatc
    16141 agcgagttcc gtaatttttt ctactgtttc ttcaataaag ctttgtccgc cgaacccagg
    16201 attgacagtc atgactagta cttgatcagc caaaggcaaa acatgtttga tcatggagac
    16261 tggtgttcct ggattgataa ctactccagc tttaactcct aagctttttg tcatttgaag
    16321 tgcccgatga atatgaggag ttgcttcgac gtggacagta atgatatctg ctccagcttt
    16381 tgcaaatgct ggaatgtagt tttctggatc aacgatcatc agatggacgt ctaacggtaa
    16441 tttagtaaca ggacggattg cctgaacaat atttggccct aatgtgatat ttgggacaaa
    16501 tcggccatcc attacatcta cgtggatgta atctgcgcct cctttttcca ctaactcaat
    16561 gtctctttgt aaattggcaa aatctgcact caaaatggat ggtgctatct tcattcttgt
    16621 ttcctcctat tttcgtttac cttctttttt tgcatacatt ggttttcttt tttcgatttc
    16681 ttgtaagaat tgcaaataat tgtcgtaacg tgtctgtgcg ataccacctg tttctactct
    16741 atgttttact tcgcagccag gttctttagc atgcatgcac tctctaaatt tgcaatggac
    16801 agaagcttct acaaattcag gaaattgtct cggcagttct cctgcttcaa ttgtcaaaaa
    16861 gtcaatcgag ctaaatcctg gtgtatctgc tactaacccg ccatagatag gaatcagttc
    16921 cacatgacgt gttgtatgcc gtcctcttcc aagtgcatca gaaatctctc ctgttgctaa
    16981 attcagttcc ggtgatattt gattcagtag cgtcgatttt cctgctccag actgacccat
    17041 aaagaccgat agccgttctg ggaagtaacg gatcagttct gtcgtatcac ccgtatgatt
    17101 gggtacaatc accggatagc ctattttagt ataggtttgc ttgatctctt cgattttttc
    17161 tgggtcgtct gacaagtcgg tctttgtcaa aaaaataatc ggttcgatcg actcgtattc
    17221 caaggtaact agaaagcggt caagtagatt ataagaaaat gcaggctcta cgatacttgt
    17281 gacaatgatt ccaagatcaa catttgtgac tggtgggcga actaattgat tttttctcgg
    17341 caagatttcc agaagatagc cgtctgtttg attttcactc tcaaagatca cctgatctcc
    17401 gacaagcggc gtgattttcc gattccggaa atttcctctt cctcttgttt gataggtctc
    17461 tccgtttgcg tatacatagt aaaaaccgct gattgctttt ctgatttgtc ctttgataag
    17521 tgccacctcc gtttttgttt attttaccat acgaagagga aagtaggtgt tattccatgc
    17581 aaacttctca aagtatatgg cttgaaaatt gatcaaaaga gatagaaaca caaaaaagag
    17641 aatgcaacaa atcaatgtcg cattcccttc tgatatattt tattcagcac ttattttgaa
    17701 ctttctgagg tgctagctga tgaacttgtg gagctggaag attcttcttt tgaagagttg
    17761 cttgtgctac tggagctact tgagctgcta gaactgtctg aagagtcttt ttcagaacta
    17821 gaagacgctg gatctggtcc tttagagtaa attacgttca caaccattcc tttggtaatt
    17881 gtcgttcctg cccctggtac tgtacgaatc actttatctt tatccaccgt gtttgaatat
    17941 tcggaagtct catgcccgat atattctgca ccgacactag ctaaataact ttgtgcttcg
    18001 ctttttgtat agccggagat atcattcaat ttaggctctg ttccttggct tacgacaaaa
    18061 ttgatgtaag tttcttctgc tacaacttca cccgaggctg gatcctgact gatgatcgaa
    18121 cctgatggta cagtgtcgct atagtcataa gtagcactaa tattattgat attgaatcct
    18181 tgtgtaacaa gccaattacg aacagtttcg tagttttctc ctacatgact actcaaagtc
    18241 actgtttctg gtcctttgct gactgtgaaa gtaatcgtgt ctgttttagg atcgacttct
    18301 gtatcttctg atggagattg cgaaatgatc attccgcttt ctacctctga actgaattcc
    18361 tcttcttttt gaatcaagtc agcagaaaac cctaattctt tcaaacttgc ttcggcatct
    18421 tcataacttt taccactgta atcatctaat ttgatttttt tcgtgccgct actaatatac
    18481 agagtgactt cgcgtttttg cttgactgtc gttcccgctg ccgggtctgt cttcactaca
    18541 ttaccttctt cgattttgtc atctgcaatt tcttctgttt ttgttgctac ttctaatttc
    18601 gcttttgcga gtgtttctct agcagaagct tcagaaagat cagaaacatc aggaacaaca
    18661 acttctcctc gcccgccaga agcaaagaat acagctgttc cgattcctaa aacagcaaga
    18721 atagctaata ggatcagcca ccattttttc tgtcctttct ttttaggatc aggcagaact
    18781 tcttcctcct ctggtaaggt ttcgttttct ggcttgtctt ttggcagagg catagatttg
    18841 aatgaatcag gcattggtgc atcttccgta aggggtgtga taacttttgt ttcattatcc
    18901 atcacatgag gctgccattt ttcttcgttt gcacgagctg gtgataatac tgtagaaaga
    18961 tcatcagaca tttcctcggc tgacttataa cggtctgcag gttctttagc cgttgcccgt
    19021 aaaatcacgt tttccagtgc ttgtggcaca tttggatcaa gtgcttttat agatggaaga
    19081 tcatcttgaa aatgttttaa agcaattgtt acagcagatt caccatcaaa cggtacgcta
    19141 cccgtcaaca tctcataaag gatgatccct aaagcataaa tatctgactg cttcgttgcc
    19201 atacttcctc tggcctgctc aggggacaga taatgaacag atcctagcat cgtattcgtc
    19261 tgcgtgatcg atgtttcaga taaagcgata gcaatcccga aatccgtgat ttttacaaca
    19321 ccttcattat cgatcagtac gttttggggt ttcaaatctc ggtggatgat ttgatggtta
    19381 tgagctaaag aaatagcaga aaggatctgc tgcataatat ttactgctgt ttcataagga
    19441 acaggataat gtgtttggat atatcgtttc aagtccattc ctttgacgta ttccatcacc
    19501 agatactgca tgttgtcttc ttcaccaaca tcgtaaacac taacaatgtt tggatgaacg
    19561 agttctgttg ctgctaaagc ttctctctga aatcgtcgaa tcgcagtctg gtcattttga
    19621 aagtcaaatc gtaagacttt aactgctaca tcacgatcca agattaaatc gtgcgccaaa
    19681 aatacatttg ccataccacc gctaccgata tttccagtga tttgataacg gccatttaat
    19741 tttttgccca actcgatcat acactgtcct ccttatattc gataagaaga acggtgatat
    19801 tatccgctcc gcctgcttca ttggcacgat caatcagctc agttacttta tgagacaatg
    19861 ggccttcttg attgatgatc gttcctattt cttcttcaga cagcatgttc gtcaagccat
    19921 ctgaacataa cagcagtcga tcttttagtt gccaaagatg agtagaaaca tctacttcga
    19981 ctgttccagg catgccgaca gaacgtgtta aaatattttt ccttggatgg ttcactgcca
    20041 tttcttcgct gatttctcct gatttgacta gttcattgac taaagaatga tcctccgtca
    20101 attgcatgat tttatcttca cgaatcaaat aagctctgct atccccaaca ttggcaatcg
    20161 tgaaccagtc atctgacaga gcggccgcta cgattgttgt tcccataccg ttatattccg
    20221 gctgttcttg tccacgctga taaatcagca tattttcttc ttgaatcgat tgaatgaacc
    20281 attgggcgat tttttcatcg tcaacagctt cttgtttttc ccaagcttcg cctagattcg
    20341 ttaccgccat ctgactggca atgtctccgg cacgatgacc acccatcccg tctgctaata
    20401 cagcaagctt gagacctgaa cggctggtaa atacactggc atagtcttga tttgtgttac
    20461 gtcgtcttcc tacatctgat tgatattcaa tctgcatttt ttcacctcat tactttttgc
    20521 gcaaacaaca aatgaaaaat ccatctgtgt aaaactgatg cggatacaaa gtcaacattt
    20581 tctcttcgat tgatgattgt agcataggat tggtcaaaac atcaatcctc tcaaaatcag
    20641 ggtgtctttc caagaaagca gaaacaacgt cttggttttc ttcttttaag atcgtacaag
    20701 tgctataagt cagaataccg gaagacttta aagttggtgc gcagctttct aaaatttcca
    20761 attggatttt tggtaaattc gctaattcat ccggctgttt tttataacga atatctggtt
    20821 ttcgccgaag cagcccaaga cctgaacaag gcgcgtctac taaaatccga tcaaactgct
    20881 ctgcttcgaa ctgtttactg acttctctag catccatctt ttgagcgtag acagcagaag
    20941 agacacctag acgtgctgca ttatcttcaa tgagtttgac tttgtgttcg tgtacatcta
    21001 aagcgaccac ttctccaccg catgacggat ctaaaaaaga agcaatatgc gtcgtcttac
    21061 ctccgggtgc tgcacaggca tctaagacat gatgttcagg ctcgatttgc atagacggcg
    21121 ctacaagcat cgaactttca tcttggatcg tcatgacgcc atcgtggaac aatgagctag
    21181 aagctaaatg cccttttttc gctactatac cgtatggact gacttggctt ttttcagctg
    21241 tgatctcttc ttcttgcaaa acagtgatgg cctgatctct ggaaagtcgg cgtgtatcta
    21301 ctcgggcact agcatggcta ggctgatata gcgataaacc gagcttttca gtttcttcgt
    21361 atccgatttg atccaccagt cgtttcgtca tccatagagg caggctgatt ttgacagaaa
    21421 gtctttcgat aggatcagca atgccttgca gatctggtgc tccttgacgc tgataattcc
    21481 tcaagacacc atttacaaat ttccctgtcc caggatttcc ttttactttt gcgatttcga
    21541 ccgcctcgtt taaaatagca tgtgcaggga ctttatctaa aaattctaat tggtatacag
    21601 aaagataaag aagcatcttg acccagtcat ccactttttt tgctttagcg atcaatggtg
    21661 caacgtaaaa ttccaatagt cgctgatggc taatcgtacc atagatcaat tctgtaaata
    21721 aacccacatc tttttgattt agctcgcttt gtttgatcat ctcattcaac aaaagattcg
    21781 agtaggcgcc acccttgttc actcgctcta atgtcagtaa tgccatatat cgtactgatt
    21841 ttttcatttt tttagggatt tttttcatta tttcacctgc tgaccaatct gaacttgctg
    21901 accgcttccg ttaaggaaat catgtacggc ctgttttcct ttaccagctg gctgtaattc
    21961 attgatggct aagacggtct gtttaccgca agcaatccat agattttttt tgtctttccg
    22021 gatgatcgta ccaggtgctt tgtctgtagt ttctgccaat acttcaaccg ataataattt
    22081 ccagcgtatg ccctcatagg tcgtgaatgc aattggccaa ggacgcattc cgcgtacttg
    22141 gttgtcaatt tcttctgcag ttttatgcca atcgattgct tcttgttccc gtgtgatgtt
    22201 tggcgaaaaa gttgcttctg actcgtcttg aggtactggt ttttgtccat ccaaaatatt
    22261 gggcaatgtt tctagcaata gctttcttcc taaaaggcta agcttttcaa acatcgtccc
    22321 tacatcatct tgtttagtga ttggtatgct ttcttgcgca tagattccgc ctgcatccat
    22381 ttttttgatc atttccatga tcgttacacc agtttctttc tccccattca tgatggcata
    22441 atgaacaggc gcacctcccc gatatttagg aagtaaagaa gcatggacat tgattgcccc
    22501 taattttggt acttgcaata acttttcagg caagaattgg ccaaatgccg cagtgacgat
    22561 cacatcaggc accaattcaa tgatttcttc catttctggc gaaccggaga ttttctctgg
    22621 ctgcagcaca cggataccgt gcttcagagc cgcttttttt accggtgtcg gcgtaattac
    22681 tcgtttccgc ccaactggac ggtcgggctg tgttaccact gcctggatct cgtaacctga
    22741 ttctatcagc ccttcaagaa tcggcacaga aaaagcaggt gtgcccataa atactagctt
    22801 agtcatcaag atgttcctcc atatattctt caagttcttc ttccgggatc tgctgtatca
    22861 ttttctcaat gaacaagata ccgtctaagt gatcgatttc atgctggaag gcacgggcta
    22921 aatagccaaa agccgtcact tctatctctt ctccgtctcg atcataataa cgcacggtca
    22981 cttcatctgc tcgcttgacc gttccataga catgcggaat actgagacaa ccttctacgt
    23041 caaggctttc accttttgct tcgatgattt ctgggttaat cagttcaaat ttctcgcctt
    23101 catctacttc gatcacagca attctcttgt tttgtcctac ttgaggagca gcgatcccta
    23161 ttccgtcatt cgcaatcatt gtctcatata aattgtctaa taaagcgatt gtctcgtccg
    23221 tgattacatc gattggctga gcagttcttt ttaatttatc attagggtgg atcaatatag
    23281 gatagcgcat aatttggccc tttcttaaat aaagtttaat ggttcgttgt caattgccac
    23341 aaaaagacct tgtcgctgtt ctttctggct tccattcaaa atttcatcca aaacatgagg
    23401 aagattcggt tcatgtttat atttgatgat tagttgataa taatatcggt ttttcactct
    23461 caaaatcatg ctgggcgttg gccctaatag caagctttga gggcttaaca cttcttttag
    23521 ctgattggca atctgaaaga ttttctttgc cgctacttgt tcttcaggat ggctggcggt
    23581 tattttcaca gtaaagtaat aaggaggata gcctccacga tgccgtacat gcatttcctg
    23641 ctgataaaac tgctcataat tttgtttttt agccaattct atggcgtaat gttgaggatt
    23701 gaatgtttgg atcacgactt cgcctgcttt ttccgctcgc cctgctcgtc ctgaaacttg
    23761 ggtaagcaat tggaatgtat gctcactaga acgaaaatcc ggtaaattca gggaggtatc
    23821 agcattcaac accccaacta atgtcacttc tggaaagtcc aaacctttag caatcatttg
    23881 tgtccctaat aatatatctg cttcttttgc accgaatttt tgtaaaatct gttcgtgtgc
    23941 cccttttctt cgagtggtat ctacatccat acgcaaaatc ctagcttgcg gataaagttc
    24001 tcggagttcc tcttctactt tttgagtacc tgttccatag tagcggattt tattgccgcc
    24061 acaattagga cagcgattgg gaataggttc ttcatgtccg caataatggc agcgcattga
    24121 acgtgtatcc atatgcagtg taagagagat atcgcaatta ggacatggaa gaacaaatcc
    24181 acagtcccta cacatcacaa aagacgaata tcctcgacga ttgagcagta ggacagtttg
    24241 ttcttttttg tctaatcgat tttgtatttt ttcctgcaaa ttagcagaaa aagtagaggt
    24301 ccgatgattc tgaaactctt cacgcatatc tacgatctca atagctggaa gctgtgcagc
    24361 tgctttggct cggtggttta actgcaataa ctgataaacg cctttttgag ctcgtgctct
    24421 tgattctaaa gagggcgtcg cgcttcctag aactattggg caatgatgat atttactgcg
    24481 ccaaattgct agatctctcg catgataacg cggtgtctca tcttgtttgt aacttgattc
    24541 atgttcttca tcgataatga tcagcccgat atttttcagc ggggcaaaaa tagcagatcg
    24601 tgcgcctact actacttgcg cttcttcccg ctcaatcttg cgccattcat catatttttc
    24661 accttgggaa agtccgctat gtaagaccgc aactgattct ccgaagcgtc ctttgaatcg
    24721 ctcgaccatt tgcggagtca atgcgatttc aggcactaac ataatagccg ttttattttc
    24781 tgacaaaaca tcagcgattg cctgaagata tacttctgtt ttcccgctgc cagtgattcc
    24841 ctccagcaaa aagacctgat ctttttgctg ctgacctgct gtgatgattt gttcgacagc
    24901 atttttttgt tctgcattca gttccaatgc cgtcgtttgg tcaaatactc ggtctttata
    24961 agggtcgcga taacgttctg attctataaa ctccagccag cccttttctt ttccttgatt
    25021 caagacagca gtactgattc ctttattttt gaaataagca attggttctt cttggctcag
    25081 ttcagataaa cacaaaagaa gttctttttg tttcttggca ttttgccgta attttgccca
    25141 ttcttcttcg atttgctttt cagttaacaa agaacggacc aaacgaatcg tttttacttt
    25201 atttcgtgta tggacttcat atttgatatc tactaattgc tcttggcgcc agcgaatcaa
    25261 ttgagaaagg ctgccttctt cttctgcttc cttccaggaa atcacttctc gctgatcaaa
    25321 caatacctga acttctggtg tgtctgccaa aggatagatc aatttatcat attctgcttt
    25381 catcacactc ggaagcatcg tttgtagaca agtgattttg aatgcgtaag taattttttt
    25441 catataatca gctaatgaaa gtaattcttc attgaccacc ggagcaaggt ctagcaaacg
    25501 aataactgcc ttcaaagatg gattagctga ttcttctgat ttttggatag ccataacaaa
    25561 tccttggaca tgacgattgc cattaccaaa agggacttct actcgcatcc cgacttgcaa
    25621 agctgtttcc atttcagagg gaaccaaata agtgaatggc tgatcggtct gcatagtagg
    25681 tacgtccaca atgacttcag ctgttaatgt catggttcgt ttcctccttt ttttgtcttt
    25741 tcttatcaag cgacaaaaag ttccttgcct attttactag agatcgatgg atttgtctta
    25801 tcttctgcca aatcctaaca aaaaattaac agcagataag aaaaaaaaga actgtgtcaa
    25861 aaagttcaga cacagccttt tctaccagac aataagtggc gaatgtttga aaaggaaaca
    25921 gctgggtttg cttttctttt tagatgttcg ctctattttt ctttattaca ttttttgctc
    25981 ttcacggata cgtgattcca attcgtgttg ttcacgctct ttttgtgctt gacgttcctc
    26041 ttcttccatc ttcaaacgtt cacgttttaa ttcaggatgt ggatcattga ctacatttcc
    26101 tgcatcgatt tcttctaaag cttgaccgac gctttttact gaatcaaaag aatctagagt
    26161 aggctgtgca cttgcatcca attcatgcgc gcgtttactt gctaaaatca ctagagaata
    26221 ttttgaattt acacgatcta atagtgagtc aattgaaggt tttaacatca taagactaca
    26281 tctcctttaa cattttaata tatttaccga ttacgcgatc tactcgaaaa tgttcactgg
    26341 caataatgtt tttgattcgt tcaactgcta gcggcacttc atcatttaca acagcatagt
    26401 cgtaaagagc catcatttcg atttcttctt ttgcaacttt cattcgtttt tcgattactt
    26461 catctgcatc tgttcctcgg cctacgatcc gtgattttaa ttccgctaaa tctggcggag
    26521 tcaaaaagat gaatacacca tcgggcactt tttctttgac ttgttttgct ccttggactt
    26581 caatctccaa gaaaacatct tttccttcat ccaatgtctt gttgacatat gacaaaggag
    26641 ttccataata attcccgaca tattctgcgt attccagcat ctctccagct tcgatcattg
    26701 attcaaactc ttctttgctt cggaagaaat aatcaacgcc ttcgacctcc ccttcacgca
    26761 ttttacgggt agtcattgag atcgaatact gaaaatcatt atcctcactc tcaaaaattg
    26821 cttttcgtac tgtgcctttt cctacaccgg atggaccaga caacacgatt aataatccac
    26881 gctctgacat gacgacgtcc tttcaactag tctttttgta cttatcgttt attttgcact
    26941 ttttttctgc aactttcaag gttatcttta attatagcta gaaattggta aaaaagctac
    27001 cctgtaccaa gaggtcgtaa aaaagctgtc ctgcatcaag taataagaac agccaaacat
    27061 aaacagcttc tcatgttttt cgtttggttg gggttattat cgcagatgca agcttttgaa
    27121 cgccgtgtat aagaggtcgt gaagaagccg attagtttcg agtatgaaga aaaattttgt
    27181 gaaaatagtt tctcacattt ttgcataaat ttggcttaat accgagaaaa tggtttttga
    27241 acatcactta ttcaaaataa aaaaagattg tgacacgaat tttgtcacaa cctttaaatg
    27301 tattcctaga gcaaagaagc taagcgaaga aatccaattc tgtttattta ttgtatttct
    27361 ttgttcagca tccttgctaa aagcccatat tggacaaagt attcactaca cccatgatct
    27421 catcttttcg agaacgtgct ttttctgtcg gatcttctac ctcttctgtg tcaattcgaa
    27481 caataaattt ccgattcatc gtcacgtaag caccgtcgtt gatcaaaata aacagttcgt
    27541 tctcattata gtctgcacag gcatctttcc atacgtcaaa gccgtttttt tcagaaggga
    27601 tatcccgttt aaccacagtt tttccactta attcgtggaa cgttacgtca taatatttca
    27661 ttatctctac ctctttcgtc attcaatatt ttgtacttgt tctctgattt tttctaagat
    27721 cgtcttagac tggacgactt gatttttaat ttcgattgca tttgatttgg aaccgatcgt
    27781 gttgatttcc cgattcattt cttggatcag gaaatctaat tcccgaccta caggacattg
    27841 tgcttcgata agttcatcta gtttttgtac atggatcgcc agacgatcca gttcctcgtg
    27901 aatatctccg cgttccaaca agatggctag ctccgtcaac aaccgttgct catcgacttt
    27961 cgctccaaga aattcctcca gctttttttg ataccttatt tgaaattcct tttcatagat
    28021 ttctacaaaa ctatcgagtt tttttagtac ttctttgaat tcttcagtat tctttttcaa
    28081 cactaaagct aaagcttgac cttctttttc tcgactcgcc tcaatagaat caagtgcgct
    28141 tttcaatgta tctagagcta actgtgccaa ttcttccatg ctctcttccg gtttttcttc
    28201 taccgttacg aagttttcgt ccagcacgaa gcgttccgcc atccgactaa ttgacacttg
    28261 attttgacta ccgtatcgtt gttgaattcc gtctgtcaat tgagtgataa gtgaatcaat
    28321 caattcccag ttgatgacga tctgtttttc gtttttgcca atataagcta gattcacaaa
    28381 tacttcgatt cgaccgcggc ttaactgctg tttaataagc tgacgaaaat cgttttcaac
    28441 aaagttcagg attttaggac ttcgtatttg aacatctaga aaacgctggt tgacactttt
    28501 tatttcaata tctaactgat acgcttttgt ttcgcggatg gctttgccaa aaccagtcat
    28561 acttttcatt cgacagggtc cttcctcatt acatattttc tattgctttt tgcagttgtt
    28621 caaactcttc gttcgtcaat gtcaggcctt tccccatttt ttcatggtct ggcgcccaat
    28681 ctcgaagatc gaattttggc gggcgaccat tccagctgac taggttcagt tctttacgcc
    28741 accccttgtt gttttctgac aatatagcga tttcttctac gatttcatat gaaaattctt
    28801 gtgccatggt tgttcctcct tgagataata taaatatagg atcagtaatg tgtcaactgg
    28861 ttgcttaagc aattcatgct gcaaacgatc cttgcgtttt ccttgtagca gataatcaaa
    28921 ataaacagtt ttcgtgcgct cattttgtat tttttgctgg tattttcgat accttcgcca
    28981 ttcttcagta cttaattgac caatttgtgc caataaatca tccggcatac tttttatatc
    29041 ttgttggcag atcatttgaa tcatcatgtc tacttgatag atttcattgg gcagtaaaaa
    29101 atgttttttt gctaaaagtt ctagcatcgc caatccgcat gtctctggta cgaccctgtt
    29161 cttatataaa ttacgcagtt tttgccaaaa ttttatgctt ttgaaaaaag ataattctaa
    29221 ctgaatttct tttgttaagt aagacaaaaa tcctcgccag tatgttcttg caggtttaac
    29281 agaatgaaat gtataccagt tcccatagta atagcctaat cgttttttca tttccagata
    29341 gcctaattgt aaatttaatt ggttcctttg gctatcaaaa agcagaaagc tgcctagtgt
    29401 ccacaatgtt tgacatttcc aatggataaa aatgtctggc atacgaattt ttttcgctgg
    29461 gcctggccct tgcacatcta cgacaaatgc ctctgttgct cctttgttaa tcgcaatatc
    29521 aataggtatt ttgttgcgat acccaccgtc tgcatattta ttttttccga tctttcgata
    29581 agccatagcg ggataaaatg aagcagaggc gagtatccaa tctggtattt cttctgtttt
    29641 tgtctgttgg atatcagtca ctacttccgc tagttctggc agtttagtag acaccgtata
    29701 taaacgtatt ttgggattat gtttgagtaa ggacaaatcc aaattgtctt tgaccatctt
    29761 ttccaatgga gcagaagaaa ccccgccttc aattagtgct gttttcgtca tttggcgtct
    29821 ttctcgatag aagcgttcac gaaggtcttc aactgcagcc atttctggta acgcaagcac
    29881 ttgtcttgtc gacagctttt tccataaatt cacagctttt ttcatatctc cttgtaaaat
    29941 caatgcgccg ttcaacgcac ctaccgaggt acctgtgata atctcaaagt tgatatcatg
    30001 ttccttcagc gcctgccaca caccaatctg ataagcaccg tgcgctcctc ctccacctaa
    30061 aacaagtgct gtatgataga ccagcttttt atttaattgt gtggttcctt gatagccgtt
    30121 tttatgaaaa atacgcaacc attcttctga tagaggagat tgaaataaaa aacaacagtt
    30181 ttctttaata aaaaaacgag cgcaagcttc catataccga aaaaattttt tccattcaaa
    30241 agtttcacct tcaatcaaca gattattaaa aataacatct cctctacttt tagcaatagt
    30301 aaaatagacg gtatattttt tcgatactgc tttaaaagca cattgctttt ttgcagaacg
    30361 ccaaaaagcg tctctacttt cttgatgact ttgaaaaaat ggaaggggtt gttttgcttg
    30421 ctggataggc gaatacagcg acattttctg tgaaaagtaa acttttttta tctccatcgc
    30481 gctctccttt ttcttcaagt ataccagtac gtcataaaaa ggtcatgagt ctcgtattcg
    30541 actttgtaga ttccataaga aaacaattca taaaacctcc tttaaataaa aaaatacaaa
    30601 aatcacaaaa attaattgaa aatgagagtg ttttcaatga aaacgtgatt ttgtaatgga
    30661 attttaaaat aaaagaaaca agctttttta atgcaaatgt tgtaaaataa aagagattaa
    30721 gaggaaataa gaggaggaac atgatgactg agtcagttta tgtacatatt gataccacaa
    30781 gtaatgctgt tttaacaaaa gggctgactg ctactgactt tgcaaacagt atcgtgcatt
    30841 ttcctcaaaa cttgctcttg cttgatccat ctgcttctgc tggagaatat gaatcccata
    30901 cagggctgaa agtaatccgt ggtacagaaa atattcagcg tttcttttcc tcttctcgta
    30961 acaggcgtgg attcgatgaa ttgaagtgga tcgactttac tgatttaacg atgctgaaag
    31021 aattgacacc tttagaaatt tcagaattat tatattttgg tcatatgaaa acacatcttc
    31081 attcgccgtt tttttataaa ctacagaata attttgtata cttcgatctt aatgatcagc
    31141 tgaatcggat ttattatcgc tatttagatg aattttatag gatttttgct agtaaattaa
    31201 gttcgatctt gtcagataag atcaatgtaa aaaaatcttt tttcaaaaaa gaaacacctg
    31261 ttgaaaaaat cagtgtggaa ttattgaaag aaatgcgtaa tttgatgcag gaagggatca
    31321 ttttttcttt tgatcaagtt ggtttgatcg atggtaaata tcgtatccca atctatgtga
    31381 ttgaagatta tatttggaaa ttaaaaggca cccggtatcg agaagaggaa atcatcgcta
    31441 ctttaatcta ctctgtcacc gatcgacact ggcaattggt agaagatgcc gatctttctt
    31501 atctgccatt gaagtagatc tatttcttat caaaaaataa aaaggaaaca agaaactagg
    31561 acatgattct gtctcaagtt tcttgcttct tttttattta cagctcctgt cgtttatctg
    31621 gcacaaaaat cgaaggagtc gtttgttgga tccctaataa aacgatgatc gttacaatag
    31681 ctgtactcaa cccgctggca ccattcatta cgaaagaaaa gagccaagga ttcatccccc
    31741 atagtgcata cgctccccag aaaaccactc ctgcaataaa atgccaaaaa tatctggaaa
    31801 gtgcgccaat caatgcacca aacacgatgc tttttctaat ttgtttcgta ttatcgtcta
    31861 catagccttt ttggacattt gaagccatga gccctgctgc gcctgcaaaa gtataggcaa
    31921 tcgggtactc aatcaacact tgaacgacac ttaaataata aacttgtccc aacggaaaat
    31981 gtaggatgcc ccaaatgaaa cctgccagca tccctgcctt tgtccctctt cttaacgcaa
    32041 aaagagtcaa tggaatctgc cctaatgaaa tagaaaaact gctgccgatc tggatcggaa
    32101 tcaacgacaa caccatcgct aacgctgcaa caatcgttcc ttctacccaa actctatttt
    32161 ttcccataaa aaaacttcct cctttgaata aaaaagtcac aaaggaggaa gcagctttcg
    32221 aaaatagtca aacagctgtt tattcacaat tcctacgctc gcataaccga tacaggtacg
    32281 aagggtttag aaatagattc aatctcagcc caattggact cccctttgtg agtttattta
    32341 ttatttattt tttgtttcaa acaatggact aactggtttg ttttcatgga tacgtttgat
    32401 tgcatctccc attagtccgc caacacttac ttcatcgatt ttgtcgattt tacgatcttc
    32461 tggaagatag atcgagtctg taacaactag acgctcgata gctgaatctt ctattctttg
    32521 taaagcaggg ccggataata ctggatgtgt acatgaagca tacacactag ttgcaccagc
    32581 ttctttcaaa gcattggcag ccaatgtgat cgtacctgct gtatcgatca tatcatcgat
    32641 cagtacacaa gttttgcctt tgacttgtcc aatgatattc attacttctg caacatttgc
    32701 ttttggacgg cgtttgtcta tgatcgcaat cggagatttc aaaaattccg ctaatttacg
    32761 tgcccgtgtg acaccaccat ggtctggtga aacaacgaca acatcatcgc ctttgattcc
    32821 tttttctaag aaataattgg caataagtgg cgcacccatc aaatgatcga ctggaatatc
    32881 aaagaaacct tggatttgga ctgcatgaag gtccaaagtc agcattcttg ttgcaccagc
    32941 tttttgaatc atgtttgcta ctaatttcgc tgtgattggt tcccgtgcac gtgctttacg
    33001 gtcttgacgc gcatagccat aataaggcat gactacgttg attgtttttg cacttgctcg
    33061 tttcagtgcg tcaatcatga tcagcaactc catcaagtta tcattgactg ggctgctcgt
    33121 tgattgaata acatagacat gcgatccacg aatactttct tcaatgttta cttgaatttc
    33181 tccatcacta aattgagtaa cagatgattt tcctaactcg acgccgactg ctgctgcaat
    33241 tttctctgct aatgggcgat tggaatttaa cgcaaaaatt tttaatcttg ggtcaaagta
    33301 atgttttgac atggggacct ccactttctt tttcacaact ctagaattct acctaataaa
    33361 tagtagtcat attttttaaa taaatcaagt gattttgcaa aagtcttagt ttagataagg
    33421 gagttttttt gcatatcctt ctttattgac ttgtcttgct ctcgcaattg ccagcgcata
    33481 ttctggaata ttgtctgtaa ttgttgaccc ggcagcaatc gaactgtttt tagctacctc
    33541 gacaggtccg atgatattcg tgctagaacc aataaagctg tggtcgccga cagtcgtatg
    33601 atgtttgttc tttccatcat agttgacgaa gacaacacca catccgacat tgatttcttc
    33661 tcccagcgta gcatcaccaa cataagtcaa gtgaccgact ttagttcctt tgccgatttg
    33721 agcatttttc acttccacaa agtttccgat atggacgttt tcgccaattt cagcttttgg
    33781 tcgtaaatgt gcataaggtc ccacatctgc atgtttcttg acatggctgc tttcaatgac
    33841 tgagttttca atgacgacgt gatcctcaat cctactgtct acgatttttg aatgtgaccc
    33901 gatgacacaa tcagaaccaa tgactgtatt tccttggatt tgtacacctg cttcgatcaa
    33961 tgtgtctgga ccgatttcta cgcctgcatc gatgtatgtc gttgctgaat cgacaaagct
    34021 tacgccattg accatatgca tctggttaat tcgctttctc atgatctcat ttgctttagc
    34081 taacgcgatc cgatcattga ctcccattga ttcttcaaaa tcatccgttt gataagcagc
    34141 aactgtatgg ccttcttctt ttaagatttc gatgatatct gttagatagt attcaccttg
    34201 agcattgttt gttccaactt tgcttaacgc atcgaacaaa gcttcgttgt caaaacagta
    34261 agttcctgta ttgatttctt gtacaagagc ttcttctggt gttgcatctt tttgttccac
    34321 gattttttct acgatcccaa tatggtcacg gatgattcgt ccatatcctg ttgggttttc
    34381 tgcttgtgcc gtcaagatcg ttgcacttgc attttttcct tggtgatatt caaaaagatt
    34441 gttcagcgtt tcagtagtca ataatggtgt atcgccactg ataactaacg ttgttccttt
    34501 ttttccttcc aagaaagaag cagcttgcaa aacagcatgt cctgttccca attgttctgc
    34561 ttgtaacgca tatttactgc gttctcctag ttgtgcttta acttgttctg ctccgtgtcc
    34621 aacgatcgta atcacttcat ctggtttcgt ttcactgacc cgattaatga tatgttctac
    34681 cataggttta ccagaaacgg gatgtaaaac tttatataat tttgacttca tgcgggtacc
    34741 tttccctgcc gccaaaataa ttgcataacg tgcgtccaac tgcgtcactc ctaaatatag
    34801 tttgctgatt tcagtttatc ccagtatacc cattttttca acaaaacaag tgattttctt
    34861 ttatcccagc gttttctgga taaaaaaata gatagaccat aacgggccta tctatttggt
    34921 aaccggtgct ctcctgccaa ctcctcggtc acaagtcaca ctactcataa aacatgaaaa
    34981 gctgtttttt gaaatcgttt cttgtgactt agagttaaac ggtaggctta caacctcttc
    35041 attaaacgtt tttcaatcag ctgaccttcg gtattagctc aataaacggc aaaatatgag
    35101 gagcaatttt tccgtttatt gagctaatac tcaggacagg acgctgattt cacaacctct
    35161 agataaacgg tgttcaaaag ccagtttctc ggtattaagc caaatttatg aaaaaatgtg
    35221 gaaagccatt ttcacaaaat ttttcttact actcgaaact aagcggcttt ttcacaacct
    35281 cttattctga aaaataattt cctggcacaa ccgtaatatt tttcgtttga gtatctacat
    35341 ctttgacata aagcagtgac gtataatcat cgatggcgcg atgaccgttg aattttgatt
    35401 cggcaaatac agtgatgcca acaagttctg attcgaattc ttcaatcaag ctcttcattc
    35461 cgttgactgt tccgccgcct ttcatgaaat catccactac aaggacacgt gaaccacgtt
    35521 tcaaacttct cttagacagt tccatctttt caattctttc agaagatcca gacacatagt
    35581 tgacacttac tgttgatcct tctgttattt ttgagtcccg gcgtacaata acgaatggcg
    35641 cattcagata ataggacaca gcttgggcaa taggtacgcc ttttgtggct acagtcatta
    35701 cagcatcgat tttttcacca aggtatttag aagcaatgat tcttccaact tgacgcagca
    35761 gttcaggttc tcctaacaaa tcagagagat aaacatatcc tcctggaagc agacggtctt
    35821 gttctgatag acgttctgct agagattcaa tatatttttt cgcctcttca tatgaaattt
    35881 cagggatgaa caatacacca cctgcagcac ccgggattgt ttccaaggtt ccataccctc
    35941 gttctttaaa tgttttcttg atgattccca aatcttcact gatagaagat ttagcagatt
    36001 cataacgttc ggcaaagctt gttaaggaaa tcaattcatg cgggtgattc aataaatact
    36061 gtgtcatgtc aactagacgt tcactgcgac gtactttcac ctattccaac tccttttaag
    36121 aaattttatc gttttacatt atataagttt ttcttcaaaa attcgagcat tttatgaaaa
    36181 aataagaaaa tagttcggta tttattaaaa taaaaagaag cctataaaaa atttttcgtt
    36241 tttataaggc ttcttttctt tataatttag ttattttttc ttctgaaggc aaccaatatt
    36301 cgtttgatga tatttacaat caggaacaaa gcaataaaga ctaatgtgat tgttgctcct
    36361 ggaggtgtat ctagataaaa cgagctagtt agtcctgaca gcatccctag aaatcctaca
    36421 caaacactga tagcaatcac gacattgaag cttttgccta atcgcatacc gattgctgcg
    36481 ggaagaacca tgattgcgga tatcaaaagt gcaccggcaa tcgggatcat cacagcaata
    36541 gccacaccag taatcacatt gaacatcatc gacatcaaat gaacgggtag cccatccacg
    36601 tgggcggtat cttcatcaaa agtcagaaca tacatcggcc gtttgaataa aaagaacaat
    36661 ataccgatgg caacgaataa gattcctaaa aagatgactt gctcccatgt aatggtcacg
    36721 atcgaaccaa acagatagga ttggatactt gttgctgaat tccctccgct caaattcatc
    36781 aatactaacg ctagcgccaa accaccagcc attaaaatgg caatggagat ttctgaatag
    36841 gttcggtaga tcgttcgcag atactccaat atcacagctg ctaatacaac gactagcatg
    36901 gtcgtaagtg taggattaag gttcaaaaag aatcctaacg ctacccctgc taaagagaca
    36961 tgagaaagcg tatcagccat cagcgattgt cttctgatca ctaaaaacac acctagcatc
    37021 ggggcgattc cggcaatgaa gatagcggca agaaacgctc ttctcatgaa ttcatatgaa
    37081 agcaacgcca ttgagaatcc tcctttctca ccagtcggat ctgtctgtcc gcatactgtt
    37141 tgatatcttc atgatcatgg gtgatcatta gaactgattt cccatggaca tgcgcactat
    37201 gtcggagaag acgataaaat tcatttctgg attcttcatc catacctgtt gttggttcat
    37261 caagaacaaa taagtcaggg tcagtagcaa aaatacgcgc taaactgatt cgctgctttt
    37321 gtccgccaga caattcacct attcgccgat ttcgcatatc ccacatccct acagcttcta
    37381 aagcacgctg tacatgttga tgatcttttt cagagagacg cttgaaccag cggcctcttg
    37441 gatatcttcc tgaacggact aattcgatca ccgtactagg aaaacctgca ttgaaagatg
    37501 caacttgttg aggaatataa ccgatactta atttttttcc ttcttgattt ttctcagcaa
    37561 tctttatcat tccttttgaa ggtttcagta atcctaacga tgctttcacc aaggttgact
    37621 tggctgctcc attttctcca gttaaaatga caaattcacc cgcatctaca tgataagaga
    37681 catcttctag tacaggttca tcatcatagt aaaaggtcaa atcagatatt tcgatataac
    37741 gcaccttaca acctccattc taaaacttaa ttgatacttt cttttaaagc tttcagattt
    37801 ttttccatga cagaaatata attttcgcct gctttgattt gttcattagt caagctttct
    37861 aaaggattca acacttctag cttcactcct gtttcattcg acaatgtctc agctaccttc
    37921 gaagaggcat tttcttcaaa ataaatcact ttgatgttgt ttttcttcac aaattctttt
    37981 agctcagcta agcgacttgg tgtgggttcc tgatctggtg aaacgcccga aatcgctact
    38041 tgttttaagt cgtattgatt cgctaaataa gcaaaagcag catgctgagt gacaaatgtg
    38101 cggtttgttg catcttttag ctcatcggag tatttttgat ccagtgcttc aagctttttg
    38161 atataagcat cggcattctt cgtaaaagtt tcctttttat ctggatattt ttcgcctaat
    38221 tcttcagcaa tcgttctgac ttcttttata gctaataccg gatcgagcca aacatgtgga
    38281 tcgtattcat gtgaatgacc atgcccttct tctgattctt catgttcgtc tgcttcttgc
    38341 gattctaaac ggtcgattcc tttagctgct tcgatcatca aggtctgttt gctatccact
    38401 gaatccttta acgaatccac aaaaaattcc atgtctgaac tattatacac gaaaacatct
    38461 gcatcggaaa tttctgctct ttcttttgca cttggttcga aatcatgcgg ctccgttcca
    38521 gcagggatca aaagtttcac gtttccttca tcccctacga tctcttttgt aaaatcatac
    38581 atcggataaa aagtcgtaac gatcgtcaaa tcctcttttt tgtctgcttc cttgttcgta
    38641 tttccacatg ctgcaaacag taaagcacct atcattaccg tagctgtcaa aagatatttt
    38701 ttcatttata tgctcctttc aatgaacaaa tcggaatttt acgatttatt aacgtgaact
    38761 aatgtatcaa aataaaaaaa cacagtcaat agaaatccat caatttctga tttaagcatg
    38821 tatattcttg attaaatcgt aaaatggatg agttgctgct catatctagc catttcacta
    38881 caataaaaaa ctgaagaata ccattttagg attcctcagt ttttcatcac ctattttact
    38941 tcttatattt tattgcaggg ttcgtactaa ataaacttcc tcacaaaaac cttttaaccc
    39001 attatatact ctctgcgctc ttgaataatg ttgacacaag gcaaaaacgg ttggtccgct
    39061 tccactcatc aatgcagcat ctgctccgta cttgatcata cgctctttga tttgatggat
    39121 gactggatgc cgctttcccg ttatatcttc taaactgttt cccatttttt tgatcatctg
    39181 ctgataatct tgctctcgga tggcttcagc aagtccgtgg atgtcttgat gttggatggc
    39241 ttgcgtatct acttctctaa acactgtacg tgtagagaca ctcattcttg gtttgaccaa
    39301 cacaacccag cactgtggca tagaaggtaa tacttggatt tcttcccctt tgccaccgac
    39361 aaatgccgta cttccgtaga cacagtaagg aacatctgta ccgacttcca aaccgatttc
    39421 tgccagttct tctaacgaac agcccagatt ccataattgg ttgatccctc ttagagcagc
    39481 tgcacagtct gtacttcctc cgcctaatcc agctgctact ggaatattct tcttaacaaa
    39541 gatcttgaca ccggttttga tattgtattt attttttagc aataaagcag cttgataaac
    39601 attgttccgt tcatctaccg gtaaaaatgc tttatttgtt tctacaatga ttttgtcttc
    39661 tgttatttct tcaaaaaata gatgatctgc caagtctacg ctcgacatca ccatttccaa
    39721 ctcatgatac ccatcttgcc gtctatataa tacatccagt cctaaattta tcttggctgg
    39781 cgctttttca atcaattcca tcctctcacc cattcgaaaa gtctaagtcg caagtattct
    39841 atcataggga aaaataaaag tatagcttat gtctgcctag tggttgattt tgtcatcgtt
    39901 tcaacagctt tcacagaatt ttctcttcat aatccaaaca agttctagtg attcttataa
    39961 ttctgtgagc acttcttctg ccaaactcgg aacatttcct ttttcttttc ctatcagact
    40021 gagtagctgc atggtagcaa atccgaaatt acttcctttc ctataagcaa aataacgtcc
    40081 ttcccaatga tcgacatatt ttaatttctt ttcataagca gactggaagg aatagaaaaa
    40141 attaccataa ttatatacga tatgcatgac tttttcttga aagaaagaaa aatctgtttc
    40201 tccaacatta gctaatggag ctaagcctag attggcttcc gagtagccca ttttctcgta
    40261 tacaataaac agattggcta acagatagtc agctaattcc tcagattctt taggtaagac
    40321 ccgcaacaaa tcatatcccg cctgcttctt cgtcatcggc tgagcggtga tgaagccaac
    40381 gactttttga tctttacgga caataccgac accgctagat aatacatatg atttatcaaa
    40441 tcgtcccaca gagaagtatt tttctcgtgc accttgaagc catttttgtg aaacttcatt
    40501 tacagcttga tgtaattctt ccggcagttg atcgtaccaa gtaaaatggt atccttcttt
    40561 catcaaacgt tgatattcaa gttgctgact cagccgagac aagtcttctg ttgctagatc
    40621 aacaatccct gcttcaccta ctttcgtgaa gtgaaaaccc aattcatgaa gcatcactac
    40681 gtagctttct gatatcttat aaaacgcaag ctgatagcct aaacaatctg cttgtttcat
    40741 aaactgattc gttgctgccc gcatcatttt aggattgcca actggatctc ctaaaacaaa
    40801 gcatttgttt cctttgatct gataaccaaa aacgacttga tcttggtcgt cttcttggta
    40861 ataaaaataa ctgtaacctg acaagcgaag ctgatggctt gtagctgtgc ctccgtatgt
    40921 ttccaataaa cggtcaaaac ggtctttttc gtactcactg ccaagcttcg tagtatcttc
    40981 agctaaatat tgataaagcg ccactaaagc tagcaatgag atgcccaaac caataagccc
    41041 tgagaaccac acatcatctg aaggaaacaa aataaaatga tttcctgtta ttcgaccgac
    41101 taaaaagcgt ccgctgtgat agcctgcaat tgcgtaaacg ataaacaaaa agccgaacaa
    41161 gctagcatca aagattatag ctccccaaga ataaacgaat tttgttcgat aaaattcttt
    41221 tctggataac cacacagcta acagaatcaa gacatatacc aatatcaact gccaagataa
    41281 cgtacgtgtg attgtattta aaataccaaa agatagtaaa gcaatcgtag gcagaaatgc
    41341 ctttttgact tttactgaaa gtgctctagc aaggccgagt aataaaaagc caaccaatag
    41401 attcactgtc tgatctaaaa aattgaatga gaacggcaat aatacttgaa atagccgact
    41461 gattgcagac agattagtga ttgttgatag aagtaccatc atgatccccg caaaatagac
    41521 agcacttacg agcacgatat gcgcaacctt ttggaaaaac agtcgcggaa tgttatctaa
    41581 aaatcgattg attttgactc ctgtatgtgt gatgaacaat agaagtcccg tcataaaagg
    41641 tagaacataa taaaacaacc gataatataa tagccaaact actgctacat cttgccgaac
    41701 agacaattga ctcattccta agatcatcaa aacgtcaaaa gtccccattc cacctgggac
    41761 catcgtcagc attcctatca atgtagcaat catgaacata ggatagatcg aaagcatcga
    41821 aatatctatt ctcatcaact tgccgatgat caaaaatacc gcaagggctc ccgtccattg
    41881 tccaaaggat gccagtagca gaccaagcaa gccttttgga taaaactcga agaacaatgt
    41941 ctttctcttt agataagcaa aaaagaaaat gactggtgct aaaaaactac cgccgaaaag
    42001 ccagatccaa tagcttcgaa aaatactttc tgactgaata aaaaagacat caataaatgt
    42061 aagaaacgac catacagaca aaccagtcag cataaatagt gcgacttttg atacagtggc
    42121 taaaaccatt tttctatttg tctgctttcc ataaaaacta gcccgcagac ttgctcctac
    42181 cacaccacca aaaccggcga gattgttgat cgtgtttgtt acccacgcag caagaagaaa
    42241 ctcttttctt tccatttttg gttttccttg tttttctaag actctgatcg tcacccaatc
    42301 atacaacaac atcggagtga cacccagcag tccagccaga atcatgagca ggatcgtcat
    42361 gcggctttgt tgtcccatcc tttgccaaat atcttgccaa ctcatgccat tagcaatatt
    42421 tgcgacttga tttgccacaa agactaacac ggagccgaaa aaaatcagtt ttaaaatcag
    42481 actatgttcc tttatccatt gcagcatcaa gtatcgttta tttttcacat tttcccattc
    42541 ctaacttgta tacttttgtg tcatacccca atgttatctt atttatttta atttttccac
    42601 tttagacctg ttctttgctt gcgatgatag aaaaagaaag atatcctaat gctaaaggag
    42661 aggataacat gaaagtagga tttatcggca cagggaatat ggcacaagct attattgatg
    42721 gtttattaaa aaaaggaaca gtctcgaaag aagaggtgtt cgtttccagt ggacattttg
    42781 aaaatgcaca aaaatttgct gctgaaactg gcgtccatgc atgcaaaagt aatcaggaag
    42841 ttgcatcaca atcagatatc gttcttttag ccgtgaaacc aaaaatcatt gaaaaagtat
    42901 tggaagaact aagagaatat agccagaaag tcttattcat ttctattgct tcaggaaaat
    42961 cattaataga tttagaaaca gcgcttgaag atcctcaggc ctccatcgta cgtgtgatgc
    43021 cgaatgtcaa tgtcagcgta ggagcaggcg tttctgcgat ttgcaaaaat gaagctgcca
    43081 cggaggaaca gcttcagcaa gctacgatga tttttgaagc aatcggcagc gtctatcctt
    43141 tagcagaatc agatttcagt acattcattg gccttgctgg cagctcaccc gcattcgttt
    43201 acatgctgat cgatgcgatg ggacggactg gtgtactgaa tggtttgtcc aaacagacag
    43261 ccactgagat tgctgcaaaa gcaattatgg gttcttgtca aaaattcctt tcaagtgata
    43321 aaaatccatg ggaacttgta gaccaagttt cttctcctgg cggtacaact gttgcaggtg
    43381 tagttgcctt agaagaagca ggattcatcc cagcaatcat caaaggaatc aatacgacga
    43441 tcgagaaaga tcttgagatg ttgaataaaa aataatgata gcaatgtttt ttcgtgcctt
    43501 acaaaaaaag aagagctgtg gcttttgccg cggctcttct tttgtatttt ttattattta
    43561 gcataatcag ttgcacgagt ttcccgaata acgattactt tgatgtgtcc aggataatcc
    43621 agttcttctt cgattcgttt acgaatatca cgtactaaac gaacagcgtc taaatccgag
    43681 atttcttctg gcttgaccat tacccgaact tcgcgtccag cttgaactgc gtaacttgat
    43741 tctacaccgg cgaagctgtt cgaaatattt tccagacttc ttaaacgacg gatgtagttt
    43801 tctaatgatt cacttcgcgc accaggtctt gctgctgaga gcgcatctgc tgcagctaca
    43861 aggacagaga taacagaagt agcttccaca tctccatgat gagaagcgat cgcgttgatt
    43921 actactgggt tttctttgta cttggcagct aattccgcac cgatctcaac gtgagatcct
    43981 tcaatctcat ggtcaagtgc tttcccaata tcatggagca acccggcacg ttttgctaat
    44041 tggatatctt cgccaagctc tgctgctaag ataccagcta attttgcaac ctcgaccgaa
    44101 tgcttcaaga cgttttgtcc ataactagtt ctgaatcgca gacgtccgat gatcttgatt
    44161 agatctggat gaagtgtatg agcaccgact tcaaacgctg cctcttcccc gtattcgcga
    44221 atacgttcat ccatttcttt acgagatttc tcgaccattt cttcgatacg cgctggatgg
    44281 attcgtccat cttgtatcag tttttccaag gtcatccgtg cgatttctct tcggattgga
    44341 tcgaatccgg aaagaacaac tgcttccggt gtatcatcga tgattaaatc gataccagtc
    44401 agcgtctcaa gcgttcgaat attacgacct tctcgtccaa tgatacgacc tttcatttca
    44461 tcattcggaa gggtaacgac tgaaacagtc gtttctgaaa cttgatctgc tgcgcagcgt
    44521 tgaatcgcta atgataataa gtttttcgct ttgcgatctg cttcttcttt ggcacgctgt
    44581 tcagattctt ttaccattag agttaactca tgattcagct cttcttcagt cgatttcatg
    44641 atgacatctc gtgcgtcctc ttttgataaa ccagcaattt tttctagttc tttttgttga
    44701 tcttcaatca ttttttcaac gtctttttct cgctcttcaa ttaattgctg ttttgaacta
    44761 agtgcttctt ctttactttc aagtgagttc tcgcgttttt ctaacgagtc atctttacga
    44821 ttcagcgttt gttcgcgttg aatcaaacga ttctcttgag attttaattc taacttactt
    44881 tctctcagct cactttcaac ttcagaacgg tatttctgat tttcttcctt agcttctaac
    44941 aatgcttctt tttttagagt ttctgcttct ttttttgcac tatcgatgat tccagcagct
    45001 gatgattgtg caccagctat ttctttctcg tgacgtgact ttgcgaccat aaaccctaaa
    45061 ccaagaccga caattaaacc gatgatagcg aggagaatgt tgaataccat aaaatccacc
    45121 tccatactat ctttttcatt tttatcatct atacagcttc tatgataaat tattctaaac
    45181 tcgtcaatat gcatcttcgc atatatgtgt acaaaaaaca tgcacaactt tattctaatg
    45241 tttgtacccc gtagtgtcaa ctttaaataa acaaagaaac ggctttctac ttattcatta
    45301 aacgatggtt tttaaaataa atgacactag tttcatatag gtaaaccttt aaattgagaa
    45361 acttttctat taaattattc tttgtagatg aacagatatt tttattttat gtacccactc
    45421 gtataattaa ataactcacg atttctttat tcaataaaaa aacggcaggt caaggagagt
    45481 acctactctt cagaccagcc gtttttgttt atcataattt ttattcttct actaaaggca
    45541 attcttcttg tcctttttca tcttctggga cagcgacttc ttcaccaata ccataagctg
    45601 ctctaactag agcagaaact tctgccatca tttcaggatg attagccata tagattttag
    45661 cgttttcccg tccttgacca atccgatctt ctttataaga ataccaagca ccgcttttat
    45721 caacaatgtc tttttctact gccatgtcta gaagttctcc ttcttgtgag atccctaaac
    45781 catacatgac atctacttca gcgattttga aaggaggtgc tactttgttt tttacgactt
    45841 tgatctttgt acggttacca acaatatctg taccttgttt caattgttct gcacgacgta
    45901 cttccaaacg gatcgttgcg tagaatttca atgcgcgtcc tccaggagtg atttctggat
    45961 tcccaaacat cacgcctact ttttcacgga tttggttgat gaaaatagcg attgtttttg
    46021 ttttgttgat cgaaccagag agtttacgca atgcttgaga catcaaacgt gcttgtaacc
    46081 cgacatgtga gtcacccatt tcgccgtcga tttctgctcg tggaactaaa gcagcaactg
    46141 aatcgactac tacgatatct acagccccac ttgatactaa agcatcagcg atctctagac
    46201 cttgttctcc tgtgtcaggc tgtgaaagaa gtagttcatc gatattcaca cctaattttt
    46261 gtgcatattg cggatctaac gcatgctcag catcaatgaa agcggccgtt ccgccatttt
    46321 tttgtacttc tgcaatagcg tgtagtgcaa ctgttgtttt acctgaactt tcaggtccat
    46381 atacttcgat gatacgtcca cgggggtaac cgccgacacc taaagcgaca tctaacgcaa
    46441 gagagccact tggaattgta gagatttgtt ggtcgatttt ttcgcccaat ttcataatag
    46501 agcctttacc atagctcttt tcaatttttt tcagtgcagc atctaaggct gctttacgat
    46561 catctgccaa tacgataatc ctccttatat ttacaaatca gagcaacatt tcattatcct
    46621 tatcatatct tttttagtga aaaaaagcaa gaaaaaaccg aacaaatatt cgcatttatc
    46681 attttcttaa aagctgtttt ctcaaaagat caaatccttg cataaccgca ttatatctga
    46741 tagcagcacg atctctggca aagtgatatt tttccgaaac cacgccgtct ttagatgaaa
    46801 tagcgatcca tactgttcct gcagattgac cttctaacgt gtccggtcca gccactccgg
    46861 tgaaagctac actgtaatct gttcttgcta tctcacgtgc ttgaatggcc atctgctcca
    46921 cacattcttt gctgactgtt ccatattctg ctaatagctc cggatcaatc tccagaaaac
    46981 cagcttttgt ttggagcgag taggtaacga atccgcctgg aaatacttca gatacaccag
    47041 caatgtcgcc taatgcggct tggaaggcgc cggctgtcaa actttccgct gctgttacag
    47101 tctgcttgtt ttctttcagt agttccacta cgacttttgc tagcgaatta tcatcgccgt
    47161 atccataaaa atattcccct actcgttctt ggattttttc ttctaatgct aaaagagctt
    47221 gattccctgc ctgaacatca tttgttttca ctgtcagtcg taatgtcact tcatttggtt
    47281 tggcatatgg tgcgatcgtt ggattgatct gtgtctcaat cagatctttt agctcagtga
    47341 ccagtcgcga ttcaccaatc ccataaaagc gcaaaacttt agaaattagt ttttcttcag
    47401 aaggaaaacg ttcctctagt aaaggacgta cttgttccac aaacattggt ttcaactcat
    47461 taggcggacc gggaagaagg atatacgcat gtgtatctgt ttgataaaaa gtccctaaag
    47521 caagccctgt acgatttggt agaggaaccc cgccttcgat aatctgggac tgttgcagat
    47581 tgtttttggt catttttcga tgggttgttt caaaatatgc caataatttt ttgtatcctt
    47641 ccgtattttg tatgagagac ttaccgaggt gagccgcagt tgcttctttt gtcaggtcat
    47701 cttctgtcgg tcctaatccg ccacataaga gtatgagttc acttcttgtt tcagctagag
    47761 caatcaactc ttctaaacgc tgctgattat ctcctacaac tgtttgataa taaacttcga
    47821 tacctagatc agctagctgt tctgacaaga aagtggcatt cgtattcacg acttgtccta
    47881 gtaaaagttc tgttccgaca gctattattt ctgctttcat tacttcccgc tcccttatat
    47941 agtatacgct gtatttttat atttttttat ttaaaaagag ggcaaggagc tgtgacaaaa
    48001 atcttgtcac agctccttgt tccaaataaa cagtgttctc ctgccaaaac ctcggtcata
    48061 agtcacaata tacataaaac atgaaaagct gttttttgaa attatccctt gtgactcaga
    48121 gttagacggc aggctcacaa cctctccact aaacgatact tacattgatc ctttgaacac
    48181 accgctgttt ttgatgaagt aatccacacc tgaatagata gtaaagatca gacaagcata
    48241 aagcatgatt tgatctactg gaataccgat caggctgaat gggatattat tgataaacag
    48301 caagataata gcaaccattt gtgtagccgt cttgactttt cccggccaag ctgcagccat
    48361 gacttctcca tgttcgacaa gtagtaaacg caagcctgtg acggctaatt cacgacagac
    48421 aatgatcgct actacccaac caggggcctt tccttggtca accaacatga taaaagcagt
    48481 cataactagc attttatctg ccaacgggtc cgcaaacttg ccaaaattag tgacaaggcc
    48541 tcgcgcacgg gcaatttttc catctaacca gtctgtgata ctagctaaag caaaaataac
    48601 tgcacctact agctgtgtga cagccagtgt ggtgtctcct acgttaaatt gtccccaatc
    48661 taaaggtaaa cctacaacca aaatgaaaat cgggatcata caaattctaa gtacagttaa
    48721 tttatttggt aaattcacac tatcgactcc tttcttccct attctacgtg actatcagcg
    48781 aagtttccac cgatattatt tacttttcca aatgaattta ttcaaaggag tatttttatt
    48841 gaaattgatc tttttgaatt aacgaagaag gaccgcgaca cagatttgtc acgatccttc
    48901 ttttttatac ctacaagtaa acagatgcgt aaactaattt atacgatcat tctgcgtatt
    48961 gaagagtaag gttcacattt tttggactct gattgttttc ccctggatta actggtacgg
    49021 attctccatt cgctttgatc gttgcattgc ttgctgcacc aatcgtgatg gtcgcatttg
    49081 ttgctgtttc cggtaacacg gtactctgtg tttcatttgc tgccagtgtc ccttgataaa
    49141 cataggcatt gtctactagc acacctaacc atacacgatt gacagcagta aattcaaggg
    49201 ttacaggctt ggtagcgtct gtgatattta ctgcgatggc actgccagaa tcttctcctg
    49261 tagctattgc cattttcttt tcttcacttg attttgttgt tgattctact gtagaagact
    49321 ctttggtctg cgtgctttgc tctgaggaag aggactcctc gactgatcct tctactttaa
    49381 gcgaagaagc tgtgtctccg attattggat cagcttgacg atcttgccaa gtcatatagc
    49441 ccacgacaac gacaatcgcc aaagcaacca atcctaataa aatgacagga agacttgtcc
    49501 aaaagcgact tggatgcgat tcttcctgat gcattgcttt tctagaagta cttactgttt
    49561 caggctgcgg acgttcaggg accgatcctg tcaatttgct tttcccatca aaaacatcga
    49621 ctaaatggtc tccatcttct cctacagctt cagcgtattg ccgaataaat gcccgcacgt
    49681 aaaatttacc aggcaattgg tcaaaatcat cattgtcgat tgcttccaaa taacgttttt
    49741 gaatttttgt aatctgctgt aattcatcta aagatatttt tttattcagg cgtgcttgcc
    49801 gtaatttttc tccgatggtt tcaaaagcca ctcgttcatc ttctccagtc tgtttgattc
    49861 tccgaagcat ctgctgttgc aacgaaagac gttatgtttg ggtgctgtga caaaagtcat
    49921 gtcacagctc cttttttccg aataaacatt gttctcctgc caactcctcg gtcacaagtc
    49981 acaatatcca ataaatataa gaagctgttt tttgaacttg ttccttgtga ctcggagtta
    50041 aacggcaggc tcacgacctc tttcttaaac ggtgttcaat cagctgttcc tcggtattag
    50101 ctcaaaaacc ggcaaaatat aaggagatat ttttccgtat gtttctctaa tactcaggtc
    50161 aaaacgctga tttcacaacc tcttccttaa acggtgttca aaagcttgca tctgcggtaa
    50221 taagtccaac caagttaaaa acattaagaa gctgttttta cttggctgtt cttattactt
    50281 gatgcagggc agctttttca caacctcttg gtacacggtg ttccagaagt agcttcttcg
    50341 gcaataagcc gaaatatcac aaaaatttga gaagcaattt tcgtaaattc cttcttattt
    50401 cttgaagctg accacttctg tcacaacctc tttgattact tcgtttgatt ttattttaat
    50461 ataagttgat taaaagagag catgatttgt tttattttct cactaatttt tccaacacaa
    50521 ctaagaatac cataaaagct gattttggag gaggttccta ctacaagtct aaaacgaaat
    50581 ttaagacttt tcctcataaa atactggttt attttggtct catgtaaaac tcgctgaagg
    50641 cctcttcact tataaatgct tccccagctg ccagtacgtc tttcatttgg atcgaatcga
    50701 tgatttcagg aagatcaaat aaagttcgat cgccaaataa actttgcgta aattgattgg
    50761 caatatattc gatcgaattt aatgattgga aatattgtcc aagcattttc ttcttcagca
    50821 aatcgagatt tgtttctgac acttccggat catcagcgaa tccaagaatg atttctttga
    50881 ctttttctgc tgctttctct ggttcgtccg tatctccgct gaagtccgca aagtgaaatt
    50941 cacgatccaa gctgaattca aaaccaaagc tgtcatcgat aattccttga ttgtacatcg
    51001 ctagatagtt tcgagaagta ttgcctaaaa tcatttggaa caacagattg attgctgttt
    51061 tatagcgtaa cagttctgtt ccttcttgcg gcaatgtgtc taatccttta ataccaagta
    51121 caaatttatc tcgtgtaatg gctgcttcca atgcactttg tttgatgatt tcttttgtgt
    51181 tttcagggaa ataacgtaca atttcttgct ttggagggaa attttttgct tcttggtttt
    51241 cccgaataag tttcatcaat ttctcaggct ccatcttacc aactacaaat aaaaccatat
    51301 tgcttggctg ataaaacgtc cgatagcacg tatacaagtc ttgagctgtg attttgtcga
    51361 tactttcaac cgtgcccgca atatcgatat gcaatgggtg tgtaggatac aagttattta
    51421 gtatgccaaa aaacatccgc cagttagggt cgtcttcata catttggatt tcctgaccga
    51481 tgattccttt ttccttgttt acagtttctt ctgtgaagta aggtgcttgt acaaaatcga
    51541 tcagagtcgt cagattcttt tctacctgat ccgtcgtaga aaacagatag ctggttttcg
    51601 taaaacttgt gaaagcattg gcggaagctc cttgtttccc aaataattga aagacatcac
    51661 catcttcttt ttcaaacaac ttatgttcta aaaaatgggc aattccatct ggaactttca
    51721 ccttttcttt ttcaccatag ggaataaatt cgttatcgat cgaaccataa ttcgtactga
    51781 acaaaccata tgtcttgtga tagtcatttt taggcaacaa atacacggtc aaaccgtttg
    51841 gcagtacttc atgataaagt gtttcattga tttgctcata ttctgtttta ttcatttgca
    51901 tttcctcctg ctaaaaagaa gattgcctgc aattcgattt gctcagctac ttgttgtatc
    51961 tctttgatcg ttacgctctc catacgtttc aaccattctt catcagataa ttttgtttcc
    52021 ggcagccagc tgtccaaaaa agaagcttcg attgccgctt gcggagagtc tagagaaagc
    52081 agatattggt ttctcagcat tgcttttgtc tgagacagtt ccaactctgt tatttcacca
    52141 tttcgcaaac tttctaattg ctcgtgaatc agatgaagta cttgatttcg gttcttttca
    52201 tcgataccgg tttggacact catgaatcct cggaacgtat ccacgctact agaggcataa
    52261 taagctagac tttctttctc tctgacattc atgaaaagtt tagagtgagg aaaaccgcca
    52321 aataaaccgt taaagaccat tagcgcaaaa cgttctggtt catcgtaata cacatttgtc
    52381 tgataagcta ggttcagctt tgcttgaacg attggctcac gtacttgtcg ttccttcacg
    52441 atattcactt gaggttgggt ataaaacaga tctggataaa ctgtttcccg cgactcaaat
    52501 ggtaattgcc caactgcttc agctgctttg tccggatcaa catcgccgac aacaaaaatg
    52561 tcgatttgat catttgccat catttcttga tagtaggacg ctagcgaact agcagtgatc
    52621 ttgtccaaat cttcgactac gcccaaccct gggatctttt gttccgggct gttttcaaaa
    52681 taaagttctt gcaaggcata ggaagcaaaa gtttgtttat cttccttcaa actttccaaa
    52741 tatagacgca gattgttctt ttccaagtca aacgtcaact gatcaaactg atgattttta
    52801 atatttggat aaaataaaac ctcttttaaa aaatctaccg cttgagaaaa aaggtttgga
    52861 tcattgacat attcaccatt cacaaaactg atccctgcgt ttagccaatg taaatttcct
    52921 ttttttctaa ctgataatcc aaagcttgcg ccataaagct ctgcaagttg cgcacttaac
    52981 tttgtctgat ccgaataatt caaactattt gtttccaaca tgctgctgag aagtgaacgt
    53041 ttcgtgatga cttcttgttg cagacgcgca gtaaaacgga taaaaatccg gatcgttttg
    53101 tattgttttg ttggtaagac atgaagattc acgccttctg ccaattgata caccatattt
    53161 atatgctcct tttcacagtt gattgcccgc ttactctttt aatgggtcaa cctgcctttt
    53221 ttacttattc ctctgcttga aatttataag ttcaggcaat tcgccgtttt ttctaaatga
    53281 tgttacactt tatagtatag catagatgtt ttttatcaaa agaaacagac ggctaaaagc
    53341 gtatattgtt tttttatttt ttatttttcg atatactaaa gacaaataaa agaagggagg
    53401 gtcattcatg atcaaagaat tcaaagagtt catcatgcgt ggagatgtct tggatcttgc
    53461 tgtcggcgtt gttattggta gtgcgtttac aagcatcgta aaccaagtgg tgcaaggctt
    53521 gatcacacca ctaataggtt ggtttgtcgc aatgatcaca ggtacttctg atttagaagg
    53581 agcactttca attcttgact ggtcgccgac aaaaggcgtc acatttgcgt ttggtaacgt
    53641 tattagtgct atcattacat ttctgatcac tggttttgtc ttattcttag ttgtcaaagc
    53701 ggcaaaccgt gcgaagaatt tacgctccaa agaagagcaa gtggaagtgg aagaaactcc
    53761 tactgcagaa gattatctta gtgacatacg cgatcttctt gccaaacaag tggcagaaga
    53821 caatacacat ccagtagagc aaacaaatct ttccgatact tctaaataat aaagaatcta
    53881 aaaaaaaaga aagccgattt tcagtgtcgg ctttcttttt ttagattctt aacttgctac
    53941 ttgttccttt ttcatgtcga tatgtggaat atcatcttcc aaatacacat ctgagattgc
    54001 cttgaatcca aacgactcat aaaaagcttg taggtattct tgtgcgccga tcacgattgg
    54061 tttttctgga aaatttttct ggatcatgtt aatcgtttct tgaacaatct ttcttcctaa
    54121 accatcttta cggttgtcct gatgaacaag gacacgtcca aaatggatac tctccgcttc
    54181 ttcatatact cgcgtgtagg caaggatctt cccatcagta tcttgaaaaa acgtatgcca
    54241 agcttctcca tcaatgtcat caacttcttg ataaggacac tgttgttcca caacaaaaac
    54301 tgcgattcgt tctttgacga tcgcgtaaaa ctctgctgtt gacaattctt caaatttctt
    54361 tacctgatat tccactaagc aaacgccccc tgctttttta ttcttagcat accgattcgc
    54421 acacaaaaaa gcaaacgaac cttacgaatc tcaggctcgt ttgcttttta aactgcttca
    54481 gttaaaaatt cgatctcaac tgtccgagta agtacatctg aatagctgta agatactcgt
    54541 tcaaaagaat tttcttcagg atccaaatca acaacgaata ctgaaggata ggtttctgtt
    54601 aaaacgccgc ggcgttctgt ttgacgcttt cttcctgtct gagcaaccaa catgattggg
    54661 ctgccgatac gaccttctaa atctttcttg attgaagcta aagttgttgg catacagttc
    54721 acctctacta tcaactatac cacatatttt taaaattttc aagtttacca ta
//
LOCUS       DARBDV010000007        53177 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000007 DARBDV010000000
VERSION     DARBDV010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.007"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            119..1045
                     /gene="cysK"
                     /locus_tag="SCM56_000434"
     CDS             119..1045
                     /gene="cysK"
                     /locus_tag="SCM56_000434"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEG1109263.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            1281..1727
                     /locus_tag="SCM56_000435"
     CDS             1281..1727
                     /locus_tag="SCM56_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEG1109264.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2000..3352
                     /locus_tag="SCM56_000436"
     CDS             2000..3352
                     /locus_tag="SCM56_000436"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HEG1109265.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            3523..3810
                     /locus_tag="SCM56_000437"
     CDS             3523..3810
                     /locus_tag="SCM56_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109266.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            4036..4479
                     /locus_tag="SCM56_000438"
     CDS             4036..4479
                     /locus_tag="SCM56_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG1109267.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(4485..5669)
                     /locus_tag="SCM56_000439"
     CDS             complement(4485..5669)
                     /locus_tag="SCM56_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HEG1109268.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            5937..6635
                     /locus_tag="SCM56_000440"
     CDS             5937..6635
                     /locus_tag="SCM56_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEG1109269.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            6642..7319
                     /gene="nth"
                     /locus_tag="SCM56_000441"
     CDS             6642..7319
                     /gene="nth"
                     /locus_tag="SCM56_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEG1109270.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(7445..7978)
                     /locus_tag="SCM56_000442"
     CDS             complement(7445..7978)
                     /locus_tag="SCM56_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109271.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            8407..9747
                     /locus_tag="SCM56_000443"
     CDS             8407..9747
                     /locus_tag="SCM56_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEG1109272.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(9817..12198)
                     /locus_tag="SCM56_000444"
     CDS             complement(9817..12198)
                     /locus_tag="SCM56_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEG1109273.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(12188..12835)
                     /gene="recU"
                     /locus_tag="SCM56_000445"
     CDS             complement(12188..12835)
                     /gene="recU"
                     /locus_tag="SCM56_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEG1109274.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            12899..13438
                     /locus_tag="SCM56_000446"
     CDS             12899..13438
                     /locus_tag="SCM56_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEG1109275.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            13528..13947
                     /gene="gpsB"
                     /locus_tag="SCM56_000447"
     CDS             13528..13947
                     /gene="gpsB"
                     /locus_tag="SCM56_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEG1109276.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            13969..14336
                     /gene="rnpB"
                     /locus_tag="SCM56_000448"
     ncRNA           13969..14336
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="SCM56_000448"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            14545..15711
                     /locus_tag="SCM56_000449"
     CDS             14545..15711
                     /locus_tag="SCM56_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEG1109277.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            15757..17253
                     /locus_tag="SCM56_000450"
     CDS             15757..17253
                     /locus_tag="SCM56_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HEG1109278.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            17334..18017
                     /locus_tag="SCM56_000451"
     CDS             17334..18017
                     /locus_tag="SCM56_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEG1109279.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            18662..19852
                     /locus_tag="SCM56_000452"
     CDS             18662..19852
                     /locus_tag="SCM56_000452"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEG1109280.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            19954..20907
                     /locus_tag="SCM56_000453"
     CDS             19954..20907
                     /locus_tag="SCM56_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEG1109281.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            20976..22325
                     /locus_tag="SCM56_000454"
     CDS             20976..22325
                     /locus_tag="SCM56_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1109282.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(22508..22831)
                     /locus_tag="SCM56_000455"
     CDS             complement(22508..22831)
                     /locus_tag="SCM56_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109283.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            22960..24123
                     /locus_tag="SCM56_000456"
     CDS             22960..24123
                     /locus_tag="SCM56_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEG1109284.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            24193..25542
                     /locus_tag="SCM56_000457"
     CDS             24193..25542
                     /locus_tag="SCM56_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEG1109285.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(25587..26018)
                     /locus_tag="SCM56_000458"
     CDS             complement(25587..26018)
                     /locus_tag="SCM56_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1109286.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            26406..29048
                     /gene="alaS"
                     /locus_tag="SCM56_000459"
     CDS             26406..29048
                     /gene="alaS"
                     /locus_tag="SCM56_000459"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEG1109287.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            29248..29844
                     /locus_tag="SCM56_000460"
     CDS             29248..29844
                     /locus_tag="SCM56_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEG1109288.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            29966..31114
                     /locus_tag="SCM56_000461"
     CDS             29966..31114
                     /locus_tag="SCM56_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEG1109289.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(31152..31565)
                     /locus_tag="SCM56_000462"
     CDS             complement(31152..31565)
                     /locus_tag="SCM56_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEG1109290.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            31644..32075
                     /locus_tag="SCM56_000463"
     CDS             31644..32075
                     /locus_tag="SCM56_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HEG1109291.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(32174..32407)
                     /locus_tag="SCM56_000464"
     CDS             complement(32174..32407)
                     /locus_tag="SCM56_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEG1109292.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            32590..34260
                     /locus_tag="SCM56_000465"
     CDS             32590..34260
                     /locus_tag="SCM56_000465"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEG1109293.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            34407..34793
                     /locus_tag="SCM56_000466"
     CDS             34407..34793
                     /locus_tag="SCM56_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109294.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            34780..35478
                     /locus_tag="SCM56_000467"
     CDS             34780..35478
                     /locus_tag="SCM56_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEG1109295.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            35483..35947
                     /locus_tag="SCM56_000468"
     CDS             35483..35947
                     /locus_tag="SCM56_000468"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEG1109296.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            35944..36855
                     /locus_tag="SCM56_000469"
     CDS             35944..36855
                     /locus_tag="SCM56_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEG1109297.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            37090..37629
                     /gene="pyrR"
                     /locus_tag="SCM56_000470"
     CDS             37090..37629
                     /gene="pyrR"
                     /locus_tag="SCM56_000470"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEG1109298.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            37643..38926
                     /locus_tag="SCM56_000471"
     CDS             37643..38926
                     /locus_tag="SCM56_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEG1109299.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            38939..39865
                     /locus_tag="SCM56_000472"
     CDS             38939..39865
                     /locus_tag="SCM56_000472"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEG1109300.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            39884..41164
                     /locus_tag="SCM56_000473"
     CDS             39884..41164
                     /locus_tag="SCM56_000473"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEG1109301.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            41169..42245
                     /gene="carA"
                     /locus_tag="SCM56_000474"
     CDS             41169..42245
                     /gene="carA"
                     /locus_tag="SCM56_000474"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEG1109302.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            42250..45435
                     /gene="carB"
                     /locus_tag="SCM56_000475"
     CDS             42250..45435
                     /gene="carB"
                     /locus_tag="SCM56_000475"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEG1109303.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            45489..46265
                     /locus_tag="SCM56_000476"
     CDS             45489..46265
                     /locus_tag="SCM56_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEG1109304.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            46262..47194
                     /locus_tag="SCM56_000477"
     CDS             46262..47194
                     /locus_tag="SCM56_000477"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEG1109305.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            47191..47907
                     /gene="pyrF"
                     /locus_tag="SCM56_000478"
     CDS             47191..47907
                     /gene="pyrF"
                     /locus_tag="SCM56_000478"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEG1109306.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            47914..48603
                     /locus_tag="SCM56_000479"
     CDS             47914..48603
                     /locus_tag="SCM56_000479"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEG1109307.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQIEARLMKIEK
                     SDLCLQNHQLE"
     gene            48576..48845
                     /locus_tag="SCM56_000480"
     CDS             48576..48845
                     /locus_tag="SCM56_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109308.1"
                     /translation="MSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFIFLR
                     LVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYLMI"
     gene            48883..49524
                     /locus_tag="SCM56_000481"
     CDS             48883..49524
                     /locus_tag="SCM56_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEG1109309.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            49567..50259
                     /locus_tag="SCM56_000482"
     CDS             49567..50259
                     /locus_tag="SCM56_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HEG1109310.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            50256..51158
                     /locus_tag="SCM56_000483"
     CDS             50256..51158
                     /locus_tag="SCM56_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG1109311.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(51316..53022)
                     /gene="efbA"
                     /locus_tag="SCM56_000484"
     CDS             complement(51316..53022)
                     /gene="efbA"
                     /locus_tag="SCM56_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HEG1109312.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        17375 a         9322 c        11394 g        15086 t
ORIGIN      
        1 attaccatat atggtatatc tttgtctttt ttttgctagt caaagacgaa tcactcgcca
       61 taaatgaaca gattgtatta tgataagtaa gaagagcaaa gacaaggagg aattgaagat
      121 gacagctatc tataactcag tcaccgaatt gattggcaaa acgccaatcg tcaaattaaa
      181 taaaatcgta ccagaagatt cagcagatgt ttttgtaaag cttgagtttt ttaatccagg
      241 aggaagtgta aaagatcgta ttgctttaag tatgatagaa aaagcagaac acgatggtct
      301 actgaaacca ggtgatacta tcattgaacc tacttctgga aatacaggaa tcggattgtc
      361 gatggtagga gttgcaaaag gatataaagt aatcatcgtg atgcctgaga cgatgagtat
      421 cgaaagacgt ttattgatga aagggtacgg cgcagagttg attttgactc ctggtgcgga
      481 tggtatttct ggatcaatca gagaagcaga acgtttggca aaagaaaacg gctacttttt
      541 accattacaa tttgaaaatg aagcaaatcc tcttgtgcat gagaagacga ctggtcctga
      601 gatacatcaa gcatttggag taaatggctt ggacgcgttt gttgcaggta tcggtacagg
      661 aggaacgatc actggcgctg gccgagaatt aaaacgtgtg tatccgaaaa tcgaattaat
      721 cggggtagaa ccagcagaat ctgctatttt agaagggaaa gaagcgggac ctcacaaaat
      781 ccaaggaatc ggtacaggtt ttgttcctaa aacactggat acttctgtgt atgataaagt
      841 cctttctatt agtggagacg aagcgatgga aacagctaga gaagttgggc gaaaagaagg
      901 gattttagta ggcatctcat ctggcgctgc aattgctgcc gcgctaaaag tcgcaaaaga
      961 attaggaaaa ggcaagaagg tattagctgt ggttcccgac aatggtgaac gttatttatc
     1021 tacagccctt taccaagaga tataaggcac ttggtttttc tccagatagg tttatgtaaa
     1081 aaacttttaa agatgaaaag aacacacaaa aatttatgat gagaaaaatt atttgaatag
     1141 agacgtagaa tagtgaacgc gactgtttta cgtctttttt cattaaaata caataaaatt
     1201 gaattttctt ttcatgttaa gtggatttta aaatgtaaaa atagtatgat aagacgagaa
     1261 taacaacgtg gggtgtttac atggaaaata agttagtcga agaggttatc gaaaagttaa
     1321 aaaaagagaa cattcgaatc acacctcaac ggtacgcggt tttagaatat ttgattgaac
     1381 atcattccca tccaacggct gatgagattt ataaagcact tgaacatcgg tttccaaata
     1441 tgagtgttgc gactgtttac aataaccttc gcttatttac ttcgattgga tttgttcaag
     1501 aaatgaagta cggcgatgct tctagccgat ttgatttcag ttcaaagcgt cattatcatg
     1561 taatttgtga aaactgcggt aagattgttg attttcatta cccagggctt gaagatatag
     1621 aaatggctgc aagcagactg actgattttg agattaatga acatcggttg gaattgtatg
     1681 gactctgtcc ggattgtaaa gagtcagcaa aaaaggcgca aatgtaaaaa cagtttctac
     1741 aagtgttata cctaaaaaaa gctagctgtg taaaaaaaac gctagctttt ttattttttt
     1801 gtcttttatt cacaatctta gatataaaag tgaatattat taaaataaga atcttgtaga
     1861 tattgggttc ttatgcttga aaaggaatga aactacaaac aaacctttgt gaattcacaa
     1921 gcattaaact gttgtatccg ttttatttct gttatagtag ttttgtcgaa ggacaaaatg
     1981 ttaatcggag ggaccataaa tgaaagtagt agtaattggc tgtactcatg caggtactgc
     2041 agctgtaaaa agtattttaa aaaatcatcc agaagcagaa gtgacagtat atgaaagaaa
     2101 tgacaatatt tctttcttat cttgcggtat cgcactttat gtcggcggag tagtaaagga
     2161 cccagcaggc ttgttctact caaatcctga agaattatct tcattaggag caacagttaa
     2221 aatggaacac gatgtaacag acgtcgatac agaaaataaa acagtaacgg caaaagatct
     2281 gaaaacagga gaaacagaaa cggtttctta tgataaatta gtgatgacaa ctggttcatg
     2341 gcctattatc ccaccgatta gtggaatcga tgcgaaaaat gtactcctat gcaaaaacta
     2401 caatcaagca aatgaaatta ttgcacaagc gaaagaagca aaacgtgtag taattgttgg
     2461 tggtggatat atcggtattg aattagtaga agcttttgtc gaatctggaa aacaagtgac
     2521 attgattgat ggattagatc gcatcttgaa taaatatcta gataaaccat tcacagatat
     2581 cttagaaaaa gaattgacag accgcggtgt tactcttgcg ctaggcgaaa atgttactga
     2641 atttatcaca gatgaagaag gaaaagtgaa acaagtagct acaccaacag atacatttga
     2701 tacagatatg gtcattttat gtgtgggctt ccgtccaaat acaaaactga tcgaaggaaa
     2761 agtagataca ctgccaaacg gggcaatcaa agtgaatgaa tacatgcaga caagtaatcc
     2821 agacattttt gcagctggag actctgcagt agtcaactac aatccaagtg gcacgcaaaa
     2881 ctatattcct ttggcaacaa atgccgttcg tcaaggtttg cttgtgggaa ataacttgac
     2941 agatcataaa atggcgtatc gtggtactca aggaacttca ggactttacc tatttggctg
     3001 gacgatcggt tcgacaggtg tgaccaaaga aagcgcaact ttgaacgatc tagaagtcca
     3061 agcgactgta tttgaagata attatcgtcc agagttcatg ccaactactg aaaaagtaat
     3121 gatggagcta gtctatgaaa aaggaacgaa tcgtatcgta ggtgctcagt tcatgtccaa
     3181 atacgacatt acacaatcag caaataccat gtctttagct gttcaaaata aaatgactgt
     3241 agaagatctc gcactttcag atttcttctt ccaaccacat tttgaccgcc catggaatta
     3301 cttgaaccta cttgcacaag cagcgctagg cgaaatcgaa actgcacaat aaagaaaaag
     3361 ggttgtgaca caattttgtc acgaccctct gtcttttttt gtgaaaagta caattagttg
     3421 tctttcttcg aattttttgt cacaataagc agcaaagata aagctgttac ataaaagaac
     3481 aagcagcttt atttgatttt gaaaagaaag gtgagaaaga agatgaataa agaatatttt
     3541 gtccaacaag atgcattttt agaagattat aaaagtgctt cgaagaaatt aacgatcaat
     3601 attgatgagt tggcagatca agtactctct cagttgaatg aaacgcaaga ggaatattac
     3661 acgatgccag caaatgacgc caaagataat gtttctcatc gttttccttt cagtagacgc
     3721 atctactcag atgaaaatga cggcacattg aatacagagt acacatatga aggtcagcca
     3781 tatgtgctag aaactgatga tgatctttaa agacagtttt tgaaaaggcc gctcatcctt
     3841 aagtgatgac aaaaaatcat cacaattacc gatgactgtc tctttactat cttttaatca
     3901 gaaaaagggg ttatgacaag ctttgtcaac cccttttttt cttggtttca gattgggttt
     3961 tcctacaaaa tatttttatc tttgttgaat aggaaacaaa aaaacaatat aatgaaagaa
     4021 aaagaaggtg tagaaatgga taaaagacaa ctgctaatag aagaatatct ggatttagct
     4081 aatgagatta ttcatcgtaa taaaccagaa atggaaaaag aatttgcagg atttactcta
     4141 aatgaactag aagtgattga acatatcggg aaaatccctg atcctaacgt gacaaaatta
     4201 gcgaatgcta gttacatgac tagaggagcc atcagtaagc taacaaaaaa actgatagct
     4261 agagggatca taaacactta tcaaagcgaa gagaataaaa aagaaatcta ttttcaattg
     4321 acgccaatcg gacaaaaaat aaggaataga cacgagcaac tccatcaaaa atgggtacag
     4381 cgagatgcta ctgtatttga aaatatgtca aaagaagaat ttgacacagt atttcgtttt
     4441 atcggtcgat acagagaatt tatacgaagt agcaaatgaa gctactattt tttattgtag
     4501 tgataatagg caaaaatcat aagcaaaaat ccgaataccg tcggaagaaa tggtgctgca
     4561 gcataaagct gtcctgcgat tagcgggccg atgacgcgcg atagagattg tatactttgg
     4621 gtaccgccca ttacaagacc ttgctggtta tctgagacag aattggatag ttggccattg
     4681 aatacaggag taaaaataga atcaccgaaa ccataacaga taatcccaaa taaaaacaaa
     4741 atcggcagta gaagaattcc agataatgtg atgaatagat aggcgaggat ttcactgaac
     4801 atccctattc ttgtgatctg ctgttctgaa aattttttga gcaatcgagg cataatgaac
     4861 aattgcgaga cagtatccaa gattccaatc aaagagaagc ttaaaccaac caacccagcc
     4921 ttccactgaa aggcgtcaat cgaaaattga gaaaagatag attgaagaga gccagctgcc
     4981 aaccaaacag caaaacctgc tgtcaataaa ggaataacgg atgaaatctt gaataattgt
     5041 tgtaattggt gaaaaggtct tacatgggag aaacttaaat ctactgaacg tttttttatt
     5101 ggaaggcttt ctagcataaa tgtgtagcca taaacggcat ttaagaaagt aaagagtgct
     5161 ccaataaata taggaacaga attccccaac tcagctaata gaccccctat aataggaccc
     5221 agcgttgttc ctataccgac taaagcaccc atccatccaa aaactttggt tctttcatta
     5281 ggttccgtga tgtcggcaaa gtaagcatat aaggtggaga tttctcctgc cgtcaatcct
     5341 tcaatgatac gaccaaggaa caacatccat atcgaatttc ctaaaccaaa aagcaaatat
     5401 ccgatacttg atcctaataa actgataata aggatcggat gacgcccaaa atgatcactt
     5461 aagctaccga gaatgggggc agataagaag gcagctagag cataaacaga catgagcaaa
     5521 gtgatataga aagcttgctg atgtatggag tgagaataag gcagcgttaa aaacggaagt
     5581 acaggactga cgatagtaaa tcctaatcca gtgaataaga cagagacgta tccaaaaact
     5641 ttagaatgtt tatttgtgaa actgtacatg taaaaactcc tttatgcaat aatgttaact
     5701 tggaaacaaa aatagtgtat agtaattttg tttccttgtc aacattctaa ggaacagcga
     5761 aatctactta atttatttat tccgaaaagt gaagatacgc atcaaaaaca tgaacaaaca
     5821 aaaatcaagt agacgacatt gtaaatctga ccgatcagtg aagtaaatag aaaagaaacc
     5881 tgttcttgct tcccatgttt tttttacgta tactgtgatt gagcggagga gaaatcatgt
     5941 tgaatttaga aaattactta agcgctggac aaacagtcat atctaatctt ttacttaaaa
     6001 attatcatag gatcggtcta caatctgaag aatttttatt tgttctacag ttgcatatgg
     6061 cacagcttga aggagatact ttccctgatt tgcagatgat tgctatggat atgggaatca
     6121 aacaagatca gatttttcaa atactggatc gtctcgtaac aaatggattt atcaagatag
     6181 aaacgacgat tgtcaacggt aaaaaagcag atcgatataa tctttacccc atctatgatc
     6241 tattaggtga gtatttaaaa acacaagaaa aaaaatacga agaaaaaaca caagaaaaag
     6301 aaatccagtc agtgtatcaa ttatttgaac aagaatttgg acgcccgctt tcttcgatcg
     6361 agttccagcg gatcggacaa tggctagaag aagaccatta tcagccggaa attttgaagc
     6421 tagcactgcg ggaagccgta ttaaatcagg cttatagttt taattacgtt gaccgcattt
     6481 tgctatcgtg ggaaagaaaa aatctacgta caaaacaaca agtggaagaa gaccaaaaac
     6541 gaagaaaaca gcagcttctt caaagagaag tagaacagtc agctcaacaa gaagaattgc
     6601 cgaaagtctc actgaaaaat tggttggagg agtagcggaa tatgttaagc aaacaaaaaa
     6661 caatggaagc tctagaaacg atgtatggaa tgtttcccga agcacatgga gaactcaagc
     6721 acaataatcc atttgagtta ttgattgcag tgatcttaag tgcccaggcg acagatgtat
     6781 cagtgaataa agcaacgcca gatttgtttg cctcttttcc gacacccgat gcgttagcag
     6841 aagcttcaat cgatgaaatc attcttaaaa taaaaacgat tggtttgtat cgaaataaag
     6901 caaaaaacat caaagcgtgt gcacagcagc tgattgaacg tttcgatggg caagtgccaa
     6961 cgtcaagaga agaattgatg tctcttcctg gtgtaggcag aaagacagca aatgtcgtat
     7021 tgggggatgc attcgggata ccagcaatcg cagtagatac ccacgtggaa cgtgtctcaa
     7081 aacgcctgag gatctgcaaa ctagatgcta cggtcatgga agtagaagaa acactgatgc
     7141 ggaaagtacc acaagaatta tgggtaaaga cacatcatac actgattttt tttggtagat
     7201 atcattgcac ggcaagaaat cccaagtgcg aggtatgtcc tttgctatca atatgtcaag
     7261 atgggaaaaa tcggatgcgt ctaaaagaaa aggcgctaaa aaagaaacca cgcctgtaga
     7321 cgtggtttct ttaattgcaa ataaatgtat gaattatctt gttattatgg agaaaagctg
     7381 tttcgtttat cttttttgca tgaaaaagta aatgtacata ctcagtagct aggatggtaa
     7441 cttattacaa tttattgcga taaaaataaa agttgttatt tttattggga aaatacccga
     7501 tgtagaaaat aatgccttcc aatataccta taaaataagt ccagtattcc aaaggattat
     7561 ataaataagt gacgtatcta tctaaaggtg taataaaaat gataagtgat aataaaaaaa
     7621 taccatttat aactaaacat aaactaatgg atttgggcgg tttcaggctc atcagaaaaa
     7681 tatagattct ggtgaacaac aaccataaaa aagtgagaat cattgcatga gagcttcttt
     7741 ccataaattt tcctttaaga gtagtgctta aaagtacaga aaaaataata aatgcaagga
     7801 taggagcaat taaagtaaat ttatttaata ataaacaaaa tttttcagct tttttattct
     7861 tggaataacg aggtattgtt ttcgataaaa aaagatggtg cagacaaatt atgataatta
     7921 agcatactat cagataactc aaaatgaagc cttctttcaa aaaattattc aaaaccatat
     7981 attgtatttt ttatttgata ataacaatgg gattattaag aagtggcact aaataaaaga
     8041 ctagtgtgta tttagccgat ttaattataa acaagtaaat ggataaagta tatgtaatga
     8101 taaaagaaaa atggattttc taatttttaa gctaaatgtt agatcacaca tgtatatgct
     8161 ggatagtctt ataaaaaatc atgtaataca gcttggtaca tgatttttat ttgtagctgt
     8221 tttttacata gacacaaaaa agaaaccacg cttgtagacg tggtttcttt tttaggatct
     8281 ataatatcta gtgttggtga cgccttctgt tgccaatact ttttcttttt tctaataaac
     8341 gcaaacagac accttgaaat cctttagaat aaagtcgacg aaaaccattc aaaggagtgt
     8401 ttcaagatgt cctattcaga gtttaccaaa gagttactag atattctaga cctaaatctt
     8461 acctttcatg aagatgcctt tcgcaaagag cgcattaatg acgaaacatg ttttgttttt
     8521 gatggcacat tgacctatca accagaggaa tgcttccatt gccactatca aaataagcaa
     8581 acgatcatta aatggggctg gaaaaaagta tccatcttat tgaatgatgt cagtaactac
     8641 aagaccattg tgcggattaa caaacagcgc ttcaaatgta aacactgtgg aaagactttt
     8701 ttagcagaag attcagttag cgatcgtcgc tgctccattg cccgcagagt caaacaagcc
     8761 attcttgagc tattaagtga gcctatttct atgtctttga ttgcacgtat gaagcacatt
     8821 tctccgacca ctgtcattcg aatacttcgc agccttcggc ctaaaaccgt ttctttgaac
     8881 cagccgttac cagaagtggt gtgtttcgat gaattcaaat cggtgaaaaa cgtctcggga
     8941 gccatgagct ttgtcatgat ggacggcaag acccatcaac tgattgatat tgtggaaaat
     9001 cggcaactca atcctttacg cgactatttc ttgcgctacc ctagaaaagt ccgtgaacaa
     9061 gtacgtctag tggtttcaga tttctataca ccctatcgca cattggtgaa agagtgtttt
     9121 ccgaatgccc gaatcgtcgc cgatcgcttt catattagcc aacacatcgg tcgggccttt
     9181 accaaccacc gaattcaagt aatgaagacc tttaaaaaag gggatcgtcg gtcgaagcac
     9241 ttaaagaaat actggagact acttcaaaag aatgcgtggg aattgaatgg tcagcatcgc
     9301 tactggcgtc cgtcatttcg agatcatctg acagaagcgg agatagtcga tcggctatta
     9361 tattatgatg actccttaaa gcgaggctac gaagtctacc aggtttttct ttccgctatc
     9421 agaagacaag atgttcctga attcgtcgcg ctcctaaaag aagactacaa ggagttgcca
     9481 gagcactatc aaccagtgtt cacgaccttc aagaaatatc gaacagaaat caaacgagcg
     9541 ttgcgagttc cctattccaa cggccctata gaatgtttga acaaccatat caaagtacta
     9601 aaacgaattg cctatggctt tagaaacttt caaaactacc gagaacgaat ctttttatat
     9661 cgaggaaaat atttcaagaa aactaagaac actacccaac tgactaaagc cagaacaaca
     9721 acacggctag acaagatagc tgtctagccg tgtgagtatc tcgattattc tattgaattt
     9781 cttcaccaac actcattgac gaagagccct tttttactat ctgtttccag aagctgaggt
     9841 gctgctggat ggtgtatggt catttgcagc atccgctccg ctagttgctg gctcactgga
     9901 agaagcagga ggagcagatg aagacggtgt cgagctttct tcggatgaag aagaaggaac
     9961 cgttgttgaa gacgctggct cgcttgtttc tgctgtcgtg ctttcttttg aggactcagc
    10021 agttgattcg ctttgactag tagagctaga gctttgagtc gttgtctctg ttgttgagct
    10081 tccaggagtc tggacatagc tggaagatgg aatagttgtt gatggtgtaa ttgcattcga
    10141 tctagctgta tattgatctt tgtagtacag ttcaccgccc acacgaatta atccgcttgg
    10201 catctcccag tctgtatttg tcacgttagc tgaaacatac tgcataagct cgcgataaac
    10261 atcagaggct acgtgtgacg attctgaagt gacaggtgtc atttttttat tgtaacctgt
    10321 ccacacggaa atcgaataat ttggcgtata gcccgcaaat aggatatctg gatacacccc
    10381 agaagaaata ccaagttttg catactcgtc atctgtgtaa ttggatgttc cggtcttacc
    10441 ggcttgatat aacccagcga tttgcgcatt cgtaccagtt ccttcagtga tcgtatcttt
    10501 taaaatgtct gtgatcatat aagctgtttc tggagacatg gctgtttttc catctggctc
    10561 ataagtttct tctgttccgt cttggaagac gattttatta acatattgag gtttgtaata
    10621 agtccctcca ttggcgaaag ccgcataggc tgctgccatt tttaaggaag aagctccgta
    10681 ttttgtccca tcttgttctt ccgtattact agagatcgca ttcgattgat ggatcgtact
    10741 gtattcgatc ccaagatttt ttaagaaaga agccacttta tctgaaccga cttcattgaa
    10801 taatttgact gctggaacat tacgggataa gtaaagtgct tggcgtaaag taatggtgcc
    10861 catatattga ttatcccagt ttcctacagg agtcgaagtc ccttcatagt tatacggtgc
    10921 gtcagtaata gtttttccag tagaatattt caaatattca aaagctggtc cgtaatctgt
    10981 gacaggtttc atcgtagaac caaaatcacg ggaagtattc actgctaagt tgttgcctaa
    11041 agttacgtct tctgcgatgt gacgtccgcc aatctgggct tttactttcc cggtattggt
    11101 gtcgataagg gtggaggcaa cttgcatctc gtcgtctggg taagagacat actgatccgt
    11161 gttgacgata tcatatagtt tcttttgggc gtccagatcc aaattcgtat aaatctctaa
    11221 accgtctgtg tagacattct tatctgtttt ttcttgtact tcattgatga cttctttgac
    11281 ataattatcg acgatcttag tattgtcatc ggattgagtt agttcttgca gaccatccgt
    11341 taccggtaca ttaacagctt gatcatattc tgtttgagag attttttcgt tttgaagcat
    11401 cgtatacaaa acagtatctc gtcttttctt cgcctgatct ggataaacat aaggatcata
    11461 ggcggaaggg gcttgaggca tcccagcaag caatgctgtt tgcggtaaag aaagttctga
    11521 taatttctta ccaaagtaca tctcagaagc tgtctccatc ccgtacaagc cgtttgacat
    11581 atacacttta tttacgtagt aggtcaagat ctcttgtttc gatttttttt gttccaatct
    11641 tacagccatc cacgcttctt gggccttgcg tttcagagtc tgatcctcgg cactggtgga
    11701 gaagaaggaa agtttgatca attgctgtgt caaagtactt ccgccttgca gtccgccaga
    11761 ggtaaagtta gagagggcag aaccgatgat ccgaataggg tcaaccccga tgtgtttata
    11821 aaaacggcga tcttctaccg acacgatcgc atcttctaat gttttcggta attcattcgc
    11881 agaaattttt tcacgctttt ctgcgcctaa atcttcgaat agttcgccat cttgtgtata
    11941 tagtttggaa gatacggtag cgtctaattt tttatccgtc aattcgggcg catcttttgc
    12001 gtaataccag aaaagtccta cacctgctaa aaagaggata cagaaaaatg agagtattcc
    12061 taaaaggatc ttgataaata atcctttttt atgtgttcca gaggactttc cagaaccttt
    12121 gccagaattc ttcttcacac tctttttagg agtaggctgt ttctgtcgtc tagagcttct
    12181 agtttgttca tttgccattc ttgctttctc cttcaataga taatgttgat aaatagaagt
    12241 caaccgcgtc caaataagga atccgcggag aaataccagt tttgatctct attcctagtt
    12301 cttcgatcaa agataaaggt agtgatttgc gtcctttttc cttttgggcg tcccagtaag
    12361 caatcaaatg aacactattc aaataaaaac agcgcttcaa agacgaaaac cataacaaga
    12421 caaagcagac cccattttgt tcgaggcatg ctttcatatg ttcgatttga tgggcatgga
    12481 agtttttaaa gggaaaagat gttttgttct tggtttcttt tgcttcaaag tccaagtaaa
    12541 agcctcgata gacgccatta tagtctgtag ttgaggcttg ggtaaagtag gcttccttga
    12601 tcacagcagc acttctctta ggatagtcta ctttcacgat ttggataggg gtaggctttt
    12661 tatggataac cgccaacccg cgggaaagat aatatttatt actttcattg attgcttctt
    12721 caaagcgcat cccccgattg ccgaattcag tgatctgatt tcttttttct tgaatttttt
    12781 cgacgggaga ggcattctta taataaggtt ggccatttgg atagcgcaat accatcttta
    12841 atcacactcc taggccacat tatagcaaaa aagcggatag aaagaaaagg aatctgagat
    12901 ggataagtca aaagtaatct atgtgtctgg gtatcgaagt ttcgaactcg gtgtttttaa
    12961 agaaaatgat cctaaaatcc aagttgttaa aaatgtttta aaaaaagaac tccatcagtt
    13021 agcagaagaa gggctggaat gggtacttgt cagtgggaat ttgggcgtcg aactatgggc
    13081 tgtgcaaata gtagcggaat tgaaagaaga atatccggaa ctatctcttg gtattttgta
    13141 tcctttttct gattttggta ataactggaa tgaagggaac caagaaaaaa aacaattagc
    13201 agaacaatta gcagattatg tcgaagctgt tagccatcaa ccctaccaat cacctagtca
    13261 gctaaagaat cacactcgtt ttatacttga gcatacagtg ggctgtctgc tgatctatga
    13321 tgaagaattc cctggaaaaa cgaagtattt ttttgaagat gcacagaatt accaagcaga
    13381 tcatccctac gaaattcggc tgatcagtat ggacgatctt caaaatttta gtgaataatc
    13441 gatattgtgt ttgatttatc aacttttttc ctatacaatg aaaaaggtag aaatgttatt
    13501 acgtaaatga aaactagagg tgtaaatatg gcgaatttgg tttatagtcc taaagacatt
    13561 ttacaacaag aattcaaaac aaaaatgcgc ggctatgatc ccgttgaagt tgatgaattc
    13621 ttagacaata tcatcaaaga ttatgaagct tacagcaaag aactccttgc tttacaagaa
    13681 gaaaacgatc gattgagtgc aaaagtagct caattatcta aaactcaagg agcagctcaa
    13741 acacgcgtgc aacaaacaga ggcaccaaag agcgcagcag tgaccaattt tgatatctta
    13801 aaacgcttgt caaatcttga acgtgaagta tttggtaaaa agcttgatca acaagcaagt
    13861 gcagtcaaac cagcacaacc taatccaaat aactacacaa atgcagacac aagcttagat
    13921 gataatgaaa aaacacgtca attttaattt ttgattaatg aatttgcggt tttcgggtaa
    13981 tcgcggtctg acttgatcag gctgaggaaa gtccatgctc gcacaggctg tgatgcctgt
    14041 agtgttcgtg cttagcgaaa ccataagcta aggtactctt tggagtaacg gcaggaaaac
    14101 aagctaaggc ttcggctatg cttaagtatc cttgaaagtg ccacagtgac gaagcggctg
    14161 gggaaaccca tccggtggaa cgcggtaaac ccctcgagcg agcaacccaa acaatggtag
    14221 gggcgctttt tgtctggaag aagaacagga caaaaaggca gtataactgc agatagatgg
    14281 ttaccgcttt gttttttctc cctggaaaag caaagtacag aacatggctt atagaaaacc
    14341 gcaaacatca gggaattagg tcgtgacaaa agtaatgcgt tataagaaac aagaaaaaca
    14401 tttgaaagct atttttcaat acttcagatg aatcgttctt attacaaaac aaacttttgt
    14461 atcaccggtt attcggaatt tgagtaaaag tgtggggtag gactgatgtc ccacctcctt
    14521 tttttataaa taaggagatt gtgtatggaa aaaaacagga agttcaacct cgtcgcaact
    14581 gcggcaagcg ggctagaagc actggttggt aaagaattaa gagatcttgg aatcgaatgc
    14641 caagttgaga acggacgtgc acgttttgaa ggaacaatgg aaacgatcgc tacagctaat
    14701 ttatggttac gtacagcaga tcgagtaaaa atcgtggttg gggaattcga tgcgtatacg
    14761 tttgatgaat tgtttgaaaa cgtaaaagca ttgccttggg aagattttct tccactagat
    14821 gcagcttttc ctgtagctgg aaaatcgatc aagtcgaaac tatatagcac acctgattgc
    14881 caagcgatca caaaaaaagc aatcgttgaa cgtctccgta cctattatca ccgtccagct
    14941 tctgtaccac tgacggaaac tggcgcacat tttcagttgg aagtagcttt gctgaaagac
    15001 catgtgatgg taactctgga tacgacagga ccaagtttat tcaaacgtgg ttatcgctta
    15061 gaaaaaggtg gagcaccatt gaaagaaaac atggcagcag cacttattat gttgacaaat
    15121 tggcgaaaag accgtccatt ttatgatcct gtatgtggtt caggtacgct ttgtattgaa
    15181 gcagccttaa taggccataa tatcgcacca ggattcaatc gtgagtttgc ttgcgaatct
    15241 tgggattggt tcagtcaaga aatcatggaa aatgttcgat cagcagccga agaaaaagca
    15301 gattatgata ttgaattaga tattactggt tcagatatca atggacggat gattgaaatt
    15361 gccaaagcca atgcagaaga aatcggatta ggctcttcga ttacctttaa gcaacaagca
    15421 gtcaaagact tcaaaacgga taaagaatac ggggtcatcg tggctaatcc gccttacgga
    15481 gaacgtctag gagaagaaga aactgttcgg aaattatata aagaaatggg cgatgtattc
    15541 cgtccattaa aaacatggag taaatatatc ttgactagcg acttagcgtt tgaggaattt
    15601 tacggacaaa aagcaaccaa aaaaagaaaa ttgtacaatg gtgcattgcg tacggatcta
    15661 ttccagtatt ggggaactcg tccgcctaga aaaaaagaga aagacgaata atcttactaa
    15721 ttgattcatt caattggatg tttggaggaa tagggaatga aagaaaaaga gtttttacaa
    15781 gaagtaaaag aaatcaatct tctacaacaa gctttaggaa tcctcgattg ggatacacaa
    15841 acaggtatgc ctgaaaaagc tagtgcatac cgaagtgaag tagacagcta tctttacagc
    15901 atttattttt caaaaaaaat cggtccaaaa atcaaagaag ccattcgcta tttttctgaa
    15961 catccggaag aattgtcgga agttggaact gccgtttttg ccaaagtaaa agaagagtat
    16021 gatttggaac atcgcgtacc ggaagatctt atgagaaaat atacagctgc cgtttcttca
    16081 gcacacggac aatggcaaaa agctagagaa acacaacaat tttctgattt tcaacacgca
    16141 ttgaatcaaa atattgaatt aacaaaacag ttgatacctt actggagaaa agatgaaaag
    16201 acgaattatg atgttttgct gaatcaatac gaacccggga tgactgtcga aatccttgat
    16261 aaggtatttg atcagcttaa agaaggaatt ctttcgatcc gccgtacgct ccaagaaaaa
    16321 gggactgaac ctgaaacaga ctttttaaat cggagaatga cgaaaaaaca acagaagaga
    16381 tttgtagtga aagtaatcga acaacttggt tatgatttta gtcgtggtcg tttagatgac
    16441 actgtccacc catttatgac tggaattaat ccgaatgatg cacgaatcac gacacgatgg
    16501 aacgaaacag atttcaaaat ggctgttttt ggagtgatcc acgaggctgg tcatggtatg
    16561 tatgaacaag acatcgatga taaatatgcg tatacaccgg tttatgaagg tacatcaatg
    16621 ggtatccatg aatcacaatc gttgtttaac gagatcatta ttggaagtag tcgcagcttc
    16681 tggaaaaagc aatatccttt ctttcaagaa tgtgcagaag gaacattcga tgatattcct
    16741 tttgacaaat tttatcgttc attgaaccat acaaaatcaa gtttggttcg tatcgaggca
    16801 gacagtttga cttaccctct ccatatcatc attcgttatg aaatcgaaaa aatgattttc
    16861 aatgaagggg tagatgttgc tgaactacct gaaatatgga atcaaaaata tgaagagtat
    16921 ctgggaatac gaccaacaaa tgatgtggaa ggcatcctac aagacgttca ttggtcaggt
    16981 gcaagctttg gttattttcc ctcttatgca ttagggtaca tgtatgcggc acaattactt
    17041 cattctttgc agcaagaaat agatgtggaa aatgttctag cgtcagacga ttattctcca
    17101 atcaaaaact ggatgtcaga gcatatccat caatatggtg catctagaaa accaaatcaa
    17161 ttaatcatgg atgcaacaca tgaaccgctt aatccgcaat acctgatcga ctatatgaaa
    17221 gacctgtatt ttaatgtata tcaggtaaaa taaaatacaa tttacaaaga tagctgaaat
    17281 tcatctaatc tatagatgtt ttcagctatt tacttaagaa agaagtggaa cgaatggata
    17341 aattacaagt gatcgctgat tatagtatgg accaattagc gcatgatcaa acaggacatg
    17401 ggagcgatca taccaaaaga gttgtcaagt tagctgaaaa aattttggag acggaaccgc
    17461 aggcagaccg ctttgtgacg cttgcagcag cttacctaca tgatacgatt gatgataaag
    17521 tcgttaaaaa tgagaacgag gcaaagcaac agcttcgtgt attccttcgc tctttgccaa
    17581 tagtagagga acagatctcg atgatttttt ccattatcga aaatatgtcc ttttctaaaa
    17641 atctctctga agcggccgaa ctatctctgg aaggtaagat cgttcaagac gctgatcgta
    17701 tcgaagcatt aggagcaatc ggcattttgc gtactgctta tttcggcggt ggacatgggc
    17761 acccgatttt tgatcctgaa ctgtatccgc aaacattcaa ggacaaagaa aattaccgaa
    17821 aaggcacgac agtgatcaat catttttatg aaaaactgtt cttgctgtct gataaaatga
    17881 atactgatta cggatatgaa gaagccaaac gaagagaaac cttcatgcgt gaattcttag
    17941 aagaattttt tctggaatgg tctattgacg atgaaaattt tactccagac aattggatca
    18001 aaaataacaa aaaatgatgg aggttgtgac agaagtggtc agctccaaga tatttcggat
    18061 cattttccgt aaaagttact tctggaacac tgtttaccaa gaggtcgtga gcctgccgtt
    18121 cagctccgag tcacaaggaa caatttcaaa aaatagcttc tcgtgtttga tgaatattgt
    18181 aacttgtgat tcggaattgg caggagaaca ccgtttattc ggaagaatag gactgtgaca
    18241 agacttttgt cacagtcctt ttttcgaatt caacaagtaa aacttttaag aaatattcag
    18301 agagtaaaat taaaaattgc tgataaacga agatggaaat tttatgtatt tttattggtc
    18361 tatgcaataa ataaattaaa tattcagaat acaattgatt ttaacgaaag ggcttctatc
    18421 aatggttgtg ttgtatttat ctgcccaggt agagtaaaat agatcattat ctaaaaggta
    18481 attgttcata ctatataagt catttgaaag taaaaagata ctgtaaataa aaaatgtggt
    18541 taaataattc tcataatcag tgtaagcgtt atcaaaaata tagcatactt gctttgtaga
    18601 tgaatgacac gatatatgtt tggtatgttc tgcaagacag atgaatatgg aggtggagac
    18661 aatgtcttcc aaagaactaa caatgtattt agagaaagaa ttggatcaac tgaaagaaaa
    18721 ggggttatac aatacgatcg acatactaga aagtgaaaat ggtgcttgta tcatcgtaga
    18781 cggaaaaaag atgatcaatc tagcatccaa caattatctt ggatttgcca atcgcgaaga
    18841 attgaaaaaa gcatgtattg aagcaaccga aacttatggt gtaggagcag gagcagtccg
    18901 aacgatcaat ggttcgctga aaatccatca gcaattagaa gaaaaaatcg cagaattcaa
    18961 agggacagaa gcagcaatcg cctttcaatc tggattcaac tgcaatatgg gcgctatctc
    19021 tgcagtaatg acaaaagaag atgctatctt atcggatgaa ttaaaccatg catctattat
    19081 tgacggttgc cgcttatccg gagcaaaaat catccgaatt aaacatcaag atatgaaaga
    19141 ccttgagaaa aaagcaaagg aagcgataga aagtaagaaa tataaaaaaa tcatgtatat
    19201 cacagatggt gttttctcca tggatggaga cattgcacga ttgccggaaa ttattccgat
    19261 tgttgaaaaa tatggactga tcacctacgt cgatgatgcg cacggctcag gcgtaactgg
    19321 aaaaggtgca ggaaccgtca agcattttgg attaagcgac aaaatcgata tgcaaatggg
    19381 cacgttatcg aaagctatag gtgttgttgg gggttatgta gctggatcga aaacactaat
    19441 cgactggttg aaagctagaa gtcggccatt cctattttct acttcgctaa ctccaggtgc
    19501 agctgcagct gcattggctt cgattacttt gatgcaagaa catccggaac ttgtcgaaaa
    19561 agtttgggaa aatgctaatt attttaaaga agaattgaaa aaagtaggct ataacatcgg
    19621 tatgtctgaa acacctatta ctccagtcat tttgggagac gaaaaagtga ctcagacttt
    19681 ctctaagaag ctgatcgaac atgggattta tgctaaacca atcgtttatc caactgtgcc
    19741 tcttgggaca ggcaggatca gaaatatgcc gactgcggaa catacgaggg aaatgcttga
    19801 tgaagcagta gcagtttatc aaaaaatagg aaaagaaatg gaaattattt aactaaattt
    19861 agttttttcg tctatttaaa attagagaac atgttcacgc atatattgtt aatcatctga
    19921 agatagtagt aaaaaagatc agggaggatc atcatgaaaa aagtattggt gacaggatgt
    19981 ttaggacaaa ttggttcaga gttgatcaca cggctacgtc aagaactagg cagtgaaaat
    20041 gtcattgcga cagatatacg catgcctgaa gtcattgaga aaaacgatat ctttgagatc
    20101 ctagatgtaa tggattacga aaaaatgcgc agcatagcag aacaatatca agtggacaca
    20161 ttgattcatt tggctgctct tttgtcagct gttgcagaag caaaaccaca attagcctgg
    20221 aatttgaacg tgactgggct ggtcaatgca ttggaagtcg ctagagaatt ccagctgaaa
    20281 ttttttacgc caagttcaat cggcgcgttt ggtccaaata ctccaaaaga tcatacccca
    20341 caagatactc ttcagcgtcc tacaaccatg tatggggtaa cgaaggtagc tggagaacta
    20401 ctatgtgatt attattatgt gaaatatggc gtagatactc gtggtgtccg tttccctgga
    20461 ttgatttctt atgcaacctt gccaggaggc ggtacgacag actacgcagt cgatatttat
    20521 tattcagcaa tcaaagaagg aaagtacact agtttcattg atcaaggtac atcaatggat
    20581 atgatgtata tgccagatgc gatcgatgcc attataaaac tcatgaatgc agatcctgat
    20641 cgattggttc atcgaaatgc cttcaatata tcggcgatgt cttttgaccc ggaagagatc
    20701 aaagcatcta ttcaaaaaat cataccagat tttgaaatgg attatgatgt ggatcctgta
    20761 cgtcagtcaa tagcagaatc ttggccaaat tcactagatg tttcttgtgc gcaagaggaa
    20821 tggggctttt cgccgaaata cgacttagat gtgatgacaa aggatatgtt ggaaaaactg
    20881 aaagaaaaat tggtagagac ggtataaaac aagaaccaca tccaaaaagt tttttatact
    20941 tttgggattg gctatggtat gaaaggggac aagggatgga agaatatcaa gccacgccag
    21001 taaaagaagt aaaaatagaa aatgaattaa aacgtacgat gggatttttt actgctttat
    21061 cgactgttat gggcacagtg atcggtgcag gggtattttt taaagccgca agcgtagcag
    21121 aagttacagg atccgccagt ttgcatatgt tttcttggtt tctcggtggt atgatttctg
    21181 tctgtgcagg actaacagga gctgaactgg cagctgctat tccagaaaca ggagggatga
    21241 ttaaatatat cgaacggatt tacggaaata cagcagcttt tttattgggc tgggcgcaag
    21301 tcgtgattta ttttccagca aatgtagcag ccttgtcgat cattttcggt acacagttcg
    21361 tcaatctatt tggtctttcc cagtcaatga ttgttcccgt agctgttaca gctgctgtat
    21421 cgatcctgtt gatcaatttt ttaggttcta aagctggagg ggcttttcag tcgattacgc
    21481 ttgtctgcaa gcttattcct ttgtttgtga tcgtcatttt cggtttgttc cgacaagaag
    21541 gagtagactt ccaactgttt ccgatccaag caggagaaaa tctttcattt ttctcagcct
    21601 tgggagcggg cttgctagca acgatgttcg catacgacgg ttggatacac gtaggaaata
    21661 tttcgggaga attgaaaaag ccggcgaaag atttgccaaa agcaatttct ttaggaatca
    21721 tcggaatcat gatcgtttat ttattagtaa atgcggtgtt cttaagaacc gcatctatcg
    21781 acggagtcgc aggaaacagc aatgcggcaa gtgacgttgc aaaaatgatt tttggcggct
    21841 tcggtggaag actggtcaca gttggtatct tgatttctgt ttatggaacc atcaatggct
    21901 atacccttac tggaatgcgt ctcccttatg ttatggcaaa agaaaacaac ctgcctttta
    21961 gcaagctctt tgctaaactt catgataaaa ctaaagttcc tgtagccgca ggtatcttag
    22021 agctagtcat cgctattggc atgatgatga tcggtggttt tgatacacta acggatatgc
    22081 tgatctttgt catttggatt ttttatacga tggtctttgt tggtgtcatt cttcttcgta
    22141 aaaaagaacc tgatttgttt cgaccataca aagttccaat gtatccgttt attccactag
    22201 tagcaatcat tggagggact tttatcgtat caagcacgct tatcactcag acctttttag
    22261 cttcgatggg gatagtattg acacttgctg gtatcccgat ttatctctat ttaaaacgta
    22321 gatagctaaa acattcgaat tttattagaa ctgcacttct ctgccaaaca aaatatttta
    22381 tttaaatgag gagtacgtgg aagaaatgtc aaaagtactc gttaattttg atcacgtaaa
    22441 tgtcgttttg tctacgatga aatagcaaag taaaagacag aaatcgggct caaccgattt
    22501 ctgtctttta ctttgatttg cgtaactgaa ctattcggcg tacgccgcga taaatattta
    22561 ataaaaccaa gcaaccagaa atgaaaatgg gaagccaaat cagatagaca gggtagccaa
    22621 gcactttaga caaagcatgt ggagtcattt tatccatttc aagaagtgct tgctgccaag
    22681 tctgaagatt gaagactagc atgaaacccg aaataatcag gagaataaat cctaaataat
    22741 gaattgtagt tctttttctt acataagcga tcgtagcata aaggaacagt aaaaaaatag
    22801 gtacgccaaa aacaaagtac atattcagca tctcgacacc ccctttgtgt ttatcttact
    22861 ttactttgaa agaaatgaga aataatagcc tcgttacttg cttgaaacag caaaggactg
    22921 ctaagataag agagatacaa agaatcaagg aggagtcata tggcaagtta tatcgaaccg
    22981 attcgttttg cgctgattgt ttttccattt ttagccttag cgatctcatc tgtttttttt
    23041 atttatgaat atcgaaagta tggaacattt ctagtaacaa gggcagttat tttgtattct
    23101 ttcgtttttt atttattgtg tgcgtatttt ttggtgatct taccacttcc ggcaaaggag
    23161 acggttgccc aaatgacagg gccaaagatg gagctgcatt tatttgcttc atggcaacat
    23221 ttcagagaca acacggtatt ggtcttgtct gaacctagta cctacctgcc ggcaatgaag
    23281 cagagtgtat ttttggaacc agcttttaat gttttattac tcataccatt tggcatttat
    23341 ttaagatatt atttccggct ttcttttatg aaaacgatga ttgctagttt ctgcctttct
    23401 ctattttttg aattgacgca gctaagtgga ctttatttta tctacccaag accataccgc
    23461 ttatttgatg tgaatgattt agtgacaaat acattgggtg gaatcattgg ttttgtcgtt
    23521 acgccgttat ttacatttat gcttcctaca agaagacgaa tggatgaact ttcttatgaa
    23581 aaaggaaaga ctgtctcatt gactcgacgc ttggtcgctt atttgatcga ctggacattt
    23641 ttatctctta ttaatttggt agcggcgatt ttctttaaaa ttgcgttcga ttcaggggca
    23701 gttacttctc atatctggtg gcttttatta gaagtagggg tatactttat gattgtaccg
    23761 tttataacca atggatggac acttggcaaa aaaattgtgc gtatccgttt ggtggaagaa
    23821 gaccgcgaac gaatttcttt aaaagcattg atgatccgct acgggtatct gtactttctt
    23881 ttttatggtt taagctggtt ggggcagttg cttcagcaaa tcatgaattc gacaacgaat
    23941 acggtgttgc agatgagtgc agtattcatt tctctcggtg tcttgttact acaaggtatt
    24001 tttattttaa atatcttgct atcatttttg agaaaaaaac ggattctgtt ttatgaaaaa
    24061 gctagtcaca catatactgt tagcacgatt ggccaaacaa aggatgatga ttctcgaaat
    24121 tagctgagca aaaacgcttt gtctgtgtta taatgccaaa gtcgtgataa tttaaagaga
    24181 agaggtgcac gaatgaataa ttttgatata ttccgcttca aaccgtttat caatgaagcg
    24241 ttaaccgcaa agggcttcaa acagcccaca gaggtccagg aacgtttgat tcctttaatt
    24301 gcaaaaggaa aaaatatcat tgggcaatct caaactggta caggaaaaac acatacgttt
    24361 ttgttgcctt tgtttgaaaa aatcaatgca gaaaaagagg aagtacaagt tgttattaca
    24421 gctccaagta gggaactagc tgcacagatc tatgaagcag ctacacaaat cgctaaattt
    24481 tctacaccgg agatccgtgt ctcgaatttt gtcggcggta cagacaaaca gcgtcaatta
    24541 aatcgtctgc atcaccaaca gccacaggtt gtagtaggca cgcctggtag aatattagac
    24601 atgatgaatg aacaagcgct gaagattcac accgcatttg catttgttgt cgatgaagcg
    24661 gatatgacat tggatatggg ttttttaaat gaagtagacc aaattgcaag tcgattacca
    24721 gaaaagctgc agtttttagt tttttctgcc actattccag aaaaacttcg tccattctta
    24781 cgaaaataca tggaaaatcc aatcattgaa gaaatcaaac caaaagcagt tatttctgaa
    24841 gacattgaaa attggctgat ttcaacaaaa ggtaaagatg agaatcaaat gatttatcaa
    24901 ctgctgacaa ttggtcatcc ttatttagca atcgtatttg ccaatacaaa acagcatgtg
    24961 gatgaaattg ccgattactt gaaaaaccaa ggcttgaaag tagcaaagat tcatggagac
    25021 atcaccccaa gagagagaaa acgagtgatg cgacaagtac agaatctgga gtatcaatat
    25081 gttgtagcga ctgatttagc tgctagaggg atcgatatcg aaggtgtctc tcatgttatt
    25141 aatgcagaaa ttccagaaga tctagacttc tttatccatc gtgtcggacg tactggacgt
    25201 aatggcttaa aaggaacggc cattacgtta tattcgccaa atgatgagca agccattgat
    25261 gcgatagaaa aattgggcgt ctcatttgaa ccaaaagaaa tcaaaaacgg ggaaatcgtc
    25321 aaaacatacg atcgtaatag acgtgccaaa cgagaaaaat caaaagatgt attagatccc
    25381 acgctgattg gtcttgtgaa gaagaaaaag aaaaagatca agcctggtta taagaaaaaa
    25441 atcgattggg caattgcaga aaaaaataaa aaagaacgca aaatcgaacg cagacagcaa
    25501 agccgtactg ctcgtaaaaa taagaaaaat tcacataaat aaaaaacaag aggttgtgac
    25561 aaaaggcgtg tcacaatctc ttgtttttaa gctagacttt ttttcattcc gatacaaagt
    25621 acttcatctt caataaaagg ttctgtgcaa gtatgatacc cttgtttttc ataaaaatct
    25681 acagccgtca tttcagctac tacataacta gtctgacaac cttctgaaac cgcccgtttt
    25741 tcagcagctt caagcaactg accgcctaac ccttttcctc ggtattcttt tgctacgcaa
    25801 aatcgttccg gcttgaagca agtaggtgtt ttcttttcat aacgcaaagt agctactggt
    25861 cttttttgat caagaaatag ataataggtt tgttgcggga tatcttttcc gtcgaattct
    25921 agttctgcag gaatcccttg ttctaataca aaaacttgat atctcaaaaa gcagctagct
    25981 gcgcgtatcc attcatccgc tccaactatt tttttcatca tattcctcct tgtcatgaat
    26041 agcagagatg tattgattcc tttattctta tcgtaccaaa aatcgtggag tatgaccaaa
    26101 aggaaatgaa aataaaaatt gagatattga cagtgatcaa tagaatgcgt atactaatta
    26161 agtacgaaaa ggatatgatt tgttttttaa gactcacaga aaattctttc ttggctgaaa
    26221 aaagaatagt cactaatcaa attgctccct ttttctattt tggtagaaat accgacgcag
    26281 ctcagcgtta tcgagtttaa gaggtaatga ttacacatca ttgcaatcaa ggtggtaccg
    26341 cgagttttcg tccttggctg tgatggtgtt tttttgtatt taatttatag aaagcaggta
    26401 atgacatgaa acaattaagt agtgcacaag ttcgacaaat gtttcttgat ttcttccaat
    26461 ctaaaggaca tacgattgaa cctagtgcgt ctttgatccc cgtgaacgat ccaaccttat
    26521 tgtggatcaa ctctggtgtt gcaacattga aaaaatattt tgatggttca gttgtgccag
    26581 aaaatccaag aatcacaaat gcccaaaaat ctattcgaac aaatgatatc gaaaatgtcg
    26641 gtaaaacagc acggcaccat acgatgtttg agatgctggg taatttttcg attggtgact
    26701 atttcaaaaa tgaagccatc cactgggcat gggaattttt gactgatcca aaatggatgg
    26761 cattcgatcc agaaaaactt tatgtaacag tttatccaaa agatacagaa gcaaaacgca
    26821 tctggcatga agaagttggc ctgccactag atcacattat tgatgtagaa gacaacttct
    26881 gggatatcgg tgcaggccca tgtggtccgg atacggaaat tttctatgac cgtggagaag
    26941 cttataacga tttagcagaa gacgatcctg aaaactatcc aggaggagaa aatgaacgtt
    27001 atttagaaat ctggaactta gtattttcag aattcaacca taagcctgat aacacatttg
    27061 aaccacttcc tcataaaaac attgatacgg ggatgggatt ggaacgcatg gtctcaatcg
    27121 ttcaagatgc gtcaacgaac tttgagacag acttgtttat gcctattatc cacgccgtag
    27181 aaaagctaag caaacaagta aaatatggtg aaaacccagt aacagatatt tcttacaaag
    27241 taatcgcaga tcatattcgc gccctttcat ttgctattgg cgacggtgca ttgccatcga
    27301 atgaagggag agggtatgtc ttacgccgtt tgcttcgtcg tgctgtcatg catgggaaaa
    27361 aattaggtat tgaacaagca ttcttatatc gtttagtccc tgttgttggg gaaatcatgg
    27421 taagttatta tcctgaagta cttcaacaaa aagaatttat tgaaaaagtt atccgtacag
    27481 aagaagaacg tttcaatgaa acaatcaatg aaggtttgga aatcttaaat caagtgatcc
    27541 aagaagtaaa aaacgaaaac gggacgactc tagcaggaaa agacatcttc aaactatacg
    27601 acacatatgg cttcccggta gaattaacgg aagaggtggc agaagatgaa ggactgaaag
    27661 ttgatcatgc tggttttgaa aaagaaatgg atgcacaacg cgaacgtgct cgctctgccc
    27721 gcagtactga aaaatcaatg ggggtacaat ctgctttatt gacagatatc aaagtagaaa
    27781 gtaaattcat tggatacaca gaacaagaat cagatagtca attattggtc atcgttaaag
    27841 gtgagtctct tttgaatgaa gtcgcagaag gtgaagctca aatgatcttt gatcaaacac
    27901 ctttctatgc agaaatgggt gggcaagtag cggatcaagg atggatcatg aatgaagtag
    27961 gcgaaatcgt agcagttgtt acagatgttc aaaaagcgcc taatggtcaa tttatgcaca
    28021 ccgtagaagt aaaagcacca atgagtgaag gacaaaccta tcatctattg atcgatcaat
    28081 cgatgcgcaa tcggatcatc aaaaaccata cagcaaccca tctactgcac aaggcattga
    28141 aagaagtact tggcgagcat gcaaatcaag ctggttcttt agtcgctcct ggacatttac
    28201 gattcgactt tacccacttt ggtcaaatca cttcagatga attagctgaa atggaaagaa
    28261 tcgtcaacga aaaaatctgg gaagctatcc cagttgaaac aattgaaaca gatatcgata
    28321 cagccaaaaa catgggtgca atggctttgt ttggtgaaaa atacggacat gacgttcgag
    28381 tagtaaatat tggcggctgg tcaatcgaac tgtgcggagg cacacatgta gcaaatacag
    28441 aaaacatcgg aatcttcaaa atcgtttctg aatcaggaat tggtgcaggc gtgcgacgaa
    28501 tcgaggctgt aacaagtaaa gaagcgtacg aactacttga agaagaagaa aaacaactaa
    28561 aacaagtagc aacaattgtt aaatcacctc aattgaaaga agttgtttca aaagcggaac
    28621 aattgcaaca gcaagtaaga gagctacaaa aggaaaatga acaattagct agcaagttag
    28681 cgaatcaaca agctggtgat gtattcaaag atccagaaac gatcaatgga aaaacaatca
    28741 ttgcagcaca agtaaaagtg aaagatatga atcagctgcg tcaattagct gaccaatgga
    28801 aacaaaaaaa tatttcagat attcttgttt tagcaaacga acaagaagga aaagtcagtc
    28861 tgctagcggc aatgacgaaa gaatcaaatg aagctggact aaaagctggc gacttgatta
    28921 aagcaatcgc accgaaagta ggcggtggcg gtggtggacg tccagacatg gcacaagctg
    28981 gcgggaaaaa tcctgctggt attccagatg cacttaaagc tgcgaaagaa tgggttgcgc
    29041 aacaataggg cgtttacgtt tgacagaaaa acaaaagttc aaacattgaa cgaaacatga
    29101 ttatgggggc gaagacaaac aagtcttgcc ctcatacttt tttcaattgg atggcgcatc
    29161 taatcaaatg gtcggattcg gatggtcttt agttgacgaa agtgacagaa atttttacga
    29221 ttagtataaa taaaaagggg tgaaaaaatg aatagtagaa ataagacctt tcgactagtc
    29281 ttacgtgcga tcttgttagc aatcatcatc gtacaagcga tggttccttg gctggggttt
    29341 attccattag gattcattag tttaacaatt atccatatca cagtaattat tgctgcagta
    29401 gtattaggcc caaaagatgg catggtgatt ggcttgtttt ggggaatcgc tacaattgtt
    29461 cgtgcctatg ccatgccaac aacgccattt gatacattag tgttcacgaa tccgattatc
    29521 tcggtagttc ccagagtact tgttggctta gtagcaggct tggttttcca ctggatttat
    29581 caaaaaaata aatcaataac gatcagctca gcctttgcag gggtactggg ttcattggtc
    29641 aatacagtct tagtgttagg attcatggga ttattttata caggtgcaac ggcagatgcc
    29701 tatggtgtag actctgctct tttatttaaa accttagcag gggttgcagc aatcaatggg
    29761 ataccagaag caataggtgc gggaatcata acaccattac tagcaaaagc gttatttgca
    29821 gcaacaccgt tgaaaccaga ataatgacca tcttttaata gtaataggac gcacgataga
    29881 tagaaaaagg aagagaggag aggaaaacaa tcgtcctctc catcttttta cctgagcaac
    29941 aagcaaacta gaaggggtaa aaaacttggc taagaaacaa gaagaaaaaa agaaacaaat
    30001 accgcaacag cggacaaaaa aaggagtact agcgggtatc ttaggattgg ttacgaatct
    30061 gatcttgttt gtctctaagc taatgatcgg gcttttttca ggaagtgtct ccattatggc
    30121 tgatgcagtg aacagtctat cagatactgc ttcatccata ttgactttga tcggatttaa
    30181 aattgctgca aagccagcag atcaggaaca tccttatgga cacgaacgct tcgaatatat
    30241 cagtgggtta tttgtttcga tcatcattac ttatgtagga tttcaatttt tagagtcttc
    30301 tgctgaaaaa atcatccatc cagaaaatgt ctctttaaca cccatcgtct tttttgtcct
    30361 aatcatttcc atctttttga aatttgcaca aggcaaaatg tatcaggtta ttgcgcaatc
    30421 cattgattct gaaacattga gagctacagc aacagatagt tataatgatg tctttactac
    30481 actggctgta ttgctttcag cagcaatcga atgggtaact ggatggcgta ttgatggata
    30541 tataggtttt gctttagcat tatatatcct ttatagtgga ttgacgatgc ttcgaaattt
    30601 tgtatacgaa ctattaggaa gtcgcccaac gccagcagag attgaagaaa tggaagagca
    30661 tttaaatgct tgcgatgata tacttggcta tcatgattta ttggtgcata actatggacc
    30721 caataaacgc tttgcatcag tccacatcga agtagatgaa tcaatgaatt tgaatgtggc
    30781 tcataagatt attgatctga tagaaaaaga ctttaaaaag tctttggatg tggaacttgt
    30841 ctgtcacttg gatccaatgc ctgttgataa cgaaaaatac tataatatct tgcggcagtt
    30901 aaagcaaatt cttcttgaaa caggtacagg gcttaatatt catgatttta gagtaattca
    30961 tcaaggaaaa acgctgcaat ttgatgtagt ggtccctgag aaattccagt ttgttgatac
    31021 agaactagaa agaatcttac gtagtcaaat agaagaaaaa atcggagatt accaattgga
    31081 aattacgttt gatcacaatt atcttttata ttaaatgaga aactataaaa agactcctta
    31141 aagtttttaa tttaaagagt ctttttttct tttaattgtt tatggagagc ctgacgtgca
    31201 agattatctg ctcctttatt tttgttttcg ggcaaccatt gcaggatcaa taaagaaaat
    31261 tcggatagaa gttcttggat tctttgaagg tagggaagga agagctcgtt cttcgttgcg
    31321 tttttgtcga ccgtagaaac tagtgttttg ctgtctgaaa aacagaaaat cgtttcattc
    31381 tgtaagtcgt ttttctttag aaattctaaa gtttttaaaa agacttcgaa ctctgcttga
    31441 tggtttgtcc ctatagaaag cgggatagat agctgttctt gtttaccttg atagacaatc
    31501 aagattccac ctccactagg accagggttg ccttttgtag aggcatcaat gtatactttt
    31561 aacataaaaa gagattctcc tttttgttca aactaatgtt atggtaagat tatatcatta
    31621 ctgaatcggt gagaggttag agaatgaaaa aaaatattcg atggcagcct gaactggcac
    31681 atacgatcat ctattggtca ttgaccttga tcattctgtt ttttagtctg attctttctt
    31741 tagaaaacac aggtccttat tggaaaagca attttgtttt aggtattttt cttgtttttc
    31801 tatttctagg tttgcggcgt tcttttattt taaaatcaaa ctctgtgatt atacgttatg
    31861 ctgctttttg gaaagaccga gaaatatctg tcggacagat tcgatctttg acgccaacta
    31921 atactggggt gcaaatgatg ttgcaagatg agaaagcccc atctgttttc atgatgcgaa
    31981 aaaaagatca agtagtcctt tttgatcatt ttgaaaaaaa cggacccaaa tcagaatcaa
    32041 ttccttatcc ttatacgaaa aaaagaaaaa gctaatttta aaaatcagaa cctaaaaaaa
    32101 cgaattttta caggaaaaga aaagagtgag acgtacggtt catcccgttt agcgtctcac
    32161 tctatttgtt tatctattca tgcatactag tatcggtagt ctgaacgttg cctttcttga
    32221 catgggcagc ttgtgttcct cgattccctt cgatgatctc aaattcgacc acttgatttt
    32281 cttccaaagt tttgaatccc tcttcttcaa tcgcagtaaa atgtacaaaa atttcttctg
    32341 tgtcatcata actgataaag ccgtatcctt ttttgttgtc aaaccatttt acaattcctg
    32401 tggtcaaagc actcacgacc tttctaaagt ctgatcatag ccttcattat aaagagtatt
    32461 tataagtacg tcaaataaat taaaaacctg taattttatg ttttttttct gaataaaaaa
    32521 aggtataatt ggcatatctt aacaaagaag atattttgtt gaataaagtt cgtgaaaagg
    32581 ggtaatcaaa tgaagaatga tctgcaaatt tcacaagaaa cagagctaga accgattgtt
    32641 gaagtggcta gtaaattaga tcttaaggaa gacgatttgg aactgtatgg gaaatataag
    32701 gcgaaaatca atttttctgg aatcaatcga tcaaaggata acgctgaggg gcatttgatc
    32761 ttagttactt ccatcaatcc aacaccagct ggcgaaggaa agtcaacgat cactgtaggg
    32821 ttaggagacg cgctcaataa aatcaataaa aaatctgtta ttgcattgcg agaaccctca
    32881 cttggtcctg tgatgggaat caaaggtggt gctgcaggcg gaggatatgc acaggtattg
    32941 cctatggaag atatcaactt gcattttact ggtgacatgc atgctatcac aacagctaat
    33001 aatgcattat ctgctctttt agataatcat attcatcaag gaaatgaatt gaacatcgat
    33061 gcccgccgag tgatttggaa acgggtagtc gatctcaatg atcgtgaact gcgtaaagta
    33121 attgttggct tgggtgggcc aatccaaggt gttccaagag aagatgggtt tgatatcaca
    33181 gttgccagcg aaatcatggc tatcttgtgt cttgcggcag accttgaaga tttaaaggct
    33241 cgtcttgcaa ggatcgttgt cggctataca tttgaccgtc agcctgtgac tgctggagac
    33301 ttgaaagcag aaggcgctct ggcattactg ctgaaagatg caatcaagcc gaatcttgta
    33361 caaacaattt acggaacacc agcttttgtc cacgggggac cgtttgccaa tatcgctcat
    33421 ggctgcaata gtattttagc taccaagaca gcattgagat tagcagatta cgttgtcaca
    33481 gaggcaggat ttggtgcaga tcttggcgga gagaaatttt tagatatcaa agtacctaac
    33541 ttgaaaaaag cgccggatgc tgtggtgatt gtcgccactg tccgtgcatt gaaaatgcat
    33601 ggcggaatga aaaaagatga acttaaaaat gagaatcttg atgctttgaa agtaggtttt
    33661 gcaaatttaa agcgccatat acgtaatatg gaacaatatc agctacctgt gatcgtcgca
    33721 atcaatgaat tcgtaacaga tacagacaat gaactcacac tgttggagca cttgtgtgaa
    33781 aatcagggga ttcttgccaa gagagcgagt gtatgggcag atggagcaga aggcggcgtt
    33841 gatttagcaa aagctgtagt acgattgatt gatagaaaag aagctaatta caagccatta
    33901 taccgcctag aagaaacgat acaagaaaag acagaaacaa tcgtcaagaa aatttacgga
    33961 ggaaatggtg ttgttttcag caaaaaagcg caaaaacaaa tagaagagtt tacgaagaat
    34021 ggatgggaca aactgccgat ttgtatggca aaaacacagt attcattttc tgatgatcct
    34081 tcactgctag gtgcaccgga agatttcacc atcacgatcc gcgaaatcat cccaaaatta
    34141 ggggcaggat ttttagtagc cttgactgga gatgttatga cgatgcctgg tttgcctaaa
    34201 aaacctgctg cattgaatat ggacgtgaca aatgacggag aagtgttggg attgttttaa
    34261 gcttgattat aatctaccat cgaaggagct gtattttgac tacagcttct ttttttagac
    34321 gatagggaat taattttgtt tacactagga tcattctttt tctttattgt attttgagct
    34381 atactaaagc ataataggag ggtatgatga aaacgacatt tataagaaaa accggttttt
    34441 acggtatggg tagctcgatt cagctaagaa tcaacaatga aaaacgcagt gttctcagtc
    34501 atgaacaaaa gataacgctt gatctggaag tgccctttac gatgcaggtc accttttatt
    34561 ggctaaaaag ccccgtttat acagtgaaag aagcaaagcc tgcttatgtg atcacaatga
    34621 attacttgat tcttcaaatc tatccgtttt tatttctagc aactgggttg ggggccgttt
    34681 tcctacaaaa tttatttttc agtttttttg cagttattgt gttgattgcg ttctttttct
    34741 atataaaaaa ccgggcatat ttgatcaagg aggaaaatta tggcaaactc tgatcttttt
    34801 ttaatcagtt tcaatcgaat cgaaaaatgg atgagagacg agatgggaaa tgcacgaaat
    34861 atgggattta ccgaacttgt cagacgccta gctcaaagaa aacaattaat gatcagaaaa
    34921 tacgaagatg atcttttaca gcttgctcag ttaaggaatg cgatcgtcca tgatcggatc
    34981 gcagtcgatt ttgtcattgc agagccaaat gattggacag tcaagcgaat ccagctgatc
    35041 gaacaagaac tgattcagcc tgaaaccgtg ctgcctcgat ttgcaaaacg tgtaacaggt
    35101 tttgaacaag atcttccgtt acttgaactg ttaaagatag tagcagaaaa aaggtattca
    35161 caatttccgt tatacaataa aggaaaattt gtagcattga ttactttacg taatatcggg
    35221 ttttggctag caaaagaaag ccaaaaaggt ccggttgatt taacaaataa acgagcgttg
    35281 gatttgatca tccaaaacgg aaagtacaca aattatcatt ttgttccagc taaaacacat
    35341 atttacgagg ttgaagcgat gtttcgtgag caagggactt tggaagccgt acttatcact
    35401 aaagatggga atcccgatgg taatctgctc gggatcgtac gacctcgtga tatttataaa
    35461 caaatagaaa aggattgatt ttttgttaat tatttattgg atattcagcg ctttgctagt
    35521 aggagtggat cagttggtca aatggtggat cactgataat ctagcgcttg gagagacgaa
    35581 aaatcttata ccaggtattt tgtcactgaa tcatattcga aacactggag ccgcatggag
    35641 tatgctagaa gggaagatgt ggttctttac tatagtgaca ctcattgcag tcgttgtgat
    35701 tctcacattg atgattaaaa atcgtgaaaa aggtaatcgc tggttcatga ttggattgag
    35761 tttgattcta gctggtgcga ttggcaattt tatcgaccgt attcgtcttg gctatgtagt
    35821 agatatgttc cagacagatt ttatgagctt tccgattttt aacgtagcag atgtgacact
    35881 tgtatgtgga gtgatttgta tgttaattta tattatttta gatgaaaagg atcaaagaaa
    35941 aaaatgacag aacttaaaac gacgattcac catgaatctg gtaggatcga taaagtattg
    36001 actaatttat tcaccgatta cagtcgttcg caaattcagc tgtggctgaa aaatggagcg
    36061 gtttctgtca atggacagcc agtcaaagca aattacaaag taaaacaagg agacgagatt
    36121 actgtcttga tccccgaacc agaaacatta gatgtggtcc cagaaaatat cccgttgacg
    36181 atcgtttacg aagatgaaga tgtagcggtg ataaacaaac ctcaaggaat ggtcgttcat
    36241 ccttcagcag gtcatacgca tggcacaatg gtcaatgcgc tgatgtacca tatcaaggat
    36301 ctttctacga tcaacggcgt tattcgtcca ggtattgttc accgaatcga caaagatact
    36361 tccggactgc taatggttgc caaaaatgac caggcacacg aatcattagc tcagcaatta
    36421 aaagataaga cctctttgcg caaatatgtt gctttagtcc atggagagat tcctcacgaa
    36481 aaaggaacca tcaatgcgcc aatcggccgt tcgaaggaaa accggaaaat gcaggcgata
    36541 agagaagatg ggaagtcggc tgtcacacat tttactgtat tggaacgttt cgaaggattt
    36601 actctggttg aattaaaact agaaaccgga agaacacacc agatccgtgt gcatatgaag
    36661 tatatcggtt atccgttagc tggagatcca gtatacggac cgaagaaaac tttgaaaggc
    36721 aatggtcagt ttctccatgc gcaaatgtta ggttttacgc acccgactac aaacgaaaag
    36781 atgatctttg aagcacctct tccagaagtt tttgaaaaaa ctttagaaaa attaagaaaa
    36841 aacattgcat tttaatttag ataacggtat agtaattaac aacgaaagca taaaacttaa
    36901 tagaattcct ttaagtttag tcccgtgagg ctaagaaggg tgggagcaag gatacgtatg
    36961 gtgcgccata agtagattgc tcattgacat agtatagcta tcagtgtatc agtatacgct
    37021 cagtctaaac attcctcctg tcctcttagg gcaggagttt ttttatgcaa aaaagtaggg
    37081 ggaatgaaaa tgcaagcaaa agaagtagta gatcaggtaa ccatgaagcg agcattgact
    37141 cgaattactt atgagattat cgagagaaac catagtatcc aagatatcgt attggtaggt
    37201 atcaaaacac gaggaatcta cattgcatca agaattgccg aacgcctgaa gcagctagaa
    37261 gatatcgata ttcctgtagg agaactggat atcactttat accgagatga caaaaaagaa
    37321 aatccagaag aaccggaact tcattcttct gacatcccag tttccttaga aggaaaagaa
    37381 gttattttga ttgatgatgt tttgtataca ggacgaacga ttcgagcagc aatggacgca
    37441 gtaatggatt ttggcagacc aagaaaaatt tcattagcag tcctggtcga tcgtgggcat
    37501 cgagaattgc caatccgtgc agattatgtt gggaagaaca ttccaacatc tcgggcggaa
    37561 gagattttag tagagatgca agagcttgat ggacaagatc gcatcatgat tttgaaagag
    37621 gaagattaga ggagagagaa acatgtcgga gaaaaaagga tttcataatg atgacgtggt
    37681 gttagatatc cacgatcgcc caaccccagg acattgggta ggattaagtt tacaacactt
    37741 gttcacgatg ttcggagcaa ccgtgttagt ccccatctta gtaggtatcg atccaggtat
    37801 tgcgctagtc agttcaggac taggtacatt ggtgtactta tttactacta aaggaaagat
    37861 acctgcgtac ttaggaagca gcttcgcttt cattgcagcg atgcagcttt tgatgaaaac
    37921 agatggttat ccagcgattg cacaaggagc agtcacaact ggattagtct atttgattgt
    37981 tgcgctgatc atcaaaaaga tcggttcagc atggctggat aaaatcttgc ctccaatcgt
    38041 cgtcggtcca gtcatcatgg ttatcgggtt gggacttgcg gcaaatgcag caacaaatgc
    38101 tatgtataat cagggcacgt atgatttcaa gtatattgct gtagcgctga tcacattagg
    38161 attaacaatt ttcttcaaca tgtggctaag aggttttctc ggattgattc cgattttact
    38221 aggtattatt agcggataca tcgtagcctt gctgtttgga atcgtggata ctaagccaat
    38281 cgtagacgct gcctggtttg ctgttcctaa ctttgagatt ccctttgtac aatatgaacc
    38341 aaagctatat cttggagcaa tcacaacaat ggcacccatc gcttttgtga cgatgacaga
    38401 gcatatcggc catcttatgg tattgaataa actaactaaa cgtaatttct ttgaagaacc
    38461 gggtcttcat aagacgttga caggtgatgg attagcacaa atcgtggcag cctttgtcgg
    38521 aggtcctcct gtaacaagtt acggtgaaaa cattggtgtt ctggctatca cacgtgtcca
    38581 tagcgtgttt gtgatcggtg gagctgcagt atttgcagtc attttaggat tcgtaggaaa
    38641 attaagcgct ttgattttaa gtatccctgg accagtcatc tcaggaatca gttttatctt
    38701 atttggagta attgccgcaa gtggaatgaa gattttaatc gagaacaaaa tcgattttga
    38761 taaaaagaaa aatctgttga ttgcttcagt tatcttagta gtaggaattg gcggactcgt
    38821 ctttgaagta ggcacattta cattgtcggc aatggcatta gcaactgtac ttggcatcat
    38881 tttaaatctt atcttaccag aaacttcacg aagcgaagaa aactaaggag gaaacattat
    38941 gatcatcaaa tcagaacgta tcagcttaaa gcatttattg accgttgagg cacttactga
    39001 tcaagaagtt atgggcttga tccgcagagg gcaggaattc aagcaaggtg ctaattggac
    39061 tccgcaaaag cgccagtact ttgctacaaa cctctttttc gaaaatagta ctcgcacaca
    39121 taaaagcttc gatgtagcgg agaaaaaact agggctagaa gtgattgaat ttgaagcaag
    39181 cacaagctct gtccaaaaag gggaaacact atacgataca gtactgacga tgtcagcatt
    39241 aggcgtagac gtagcggtta tccgtcatgg tgatgagaac tattatgacg aactgataca
    39301 aagtaaaaca atccagtgtt cgattatcaa tggaggagac ggaagcggac agcatccaac
    39361 ccaatgcttg cttgatttga tgaccattta tgaagaattt ggtcattttg aaggtctgaa
    39421 agttgccatc gtaggtgata tcactcactc tcgcgtagct aaatccaata tgcagatgtt
    39481 aaaacgtcta ggcgcacaag tattcttctc aggtcctaga gaatggtacg acgatgaatt
    39541 cgaagtgtac ggacattata tgccattgga tgaactacta gatcaagttg atgtcatgat
    39601 gatgcttcgg gtgcaacacg aacgccatga tggaaaagaa agtttctcaa aagaaggcta
    39661 ccatcaggaa catgggttaa cagttgagcg tgcgaagaag ttgcaaaaac atgcaatcat
    39721 tatgcaccca gcaccagtta accgtgatgt agaattagct gattcattag tggaaggatt
    39781 acagtcgcgg atcgttcagc aaatgagcaa tggagtattc gtcagaatgg caatcttaga
    39841 agcagtgcta aatggaaaag cgtaattata ggaggatgaa aaaatgaaaa cactcattaa
    39901 aaacggtcag atcaatacac gaaaaaatat gacgacacca gcagaaattt ggatagaaga
    39961 tgggcgaatc aaagcaatcg gtacaggatt ctcagaagcc gaatttgatg aagtgtttga
    40021 tgcaaaggga caattgatca cacctggttt agtggacgtc catgttcatt tgagagagcc
    40081 aggatttaca tacaaggaga cgatcgaagc tgggactcgc tcagctgctc gtggcggttt
    40141 cactactgta tgcgcgatgc cgaatttaaa tcctgtacct gatacggcag aaaagctgag
    40201 acaagtatat gacatcatta gaaaagatgc agttgtgaag gtattgcagt atgcgccaat
    40261 cacagaaaat ctgcgaagtg agaaattagt tgatcaagaa gcactgattg aagaaggtgc
    40321 ttttgctttc acaaatgacg gtgtaggtgt ccaaacagct gggacgatgt atctggctat
    40381 gaaagaagct gcaaaaaaca ataaagccct agtagcccat acagaagatg aatctttatt
    40441 atttggcggc gttatgcacg ctggcaagaa ggcagaagaa cttggtcttc ccgggatctt
    40501 aagcgtaacc gagtcttctc aaattgctag agatctgttg ctagctgaag caactggtgt
    40561 tcattaccat gtttgccatg tttctactaa agaaagtgtc cgtgtaatca gagacgcaaa
    40621 aaaagcagga atccacgtaa cagcagaagt ttcaccccat catcttattt tgatcgacga
    40681 agatattccc gaagatttcg gcttttggaa aatgaacccg ccgctaagag gacgggagga
    40741 cagagaagct ttgatcgaag gcttgcttga cggtacgatc gattgcatcg ctacagacca
    40801 tgccccccat ggattagaag aaaaaagcca aagtttcatg aaatcaccat ttggtatcgt
    40861 aggaagcgaa acagcgtttc aactgatcta tactcatttt gtagaaactg gtcgattcac
    40921 gttggaacaa gtgatcaatt ggttggcagt taaaccggct gagatttttg gtttgaatgc
    40981 aggaacgcta acggttggtg cccccgctga tgtagcagtt tttgatatta cacaaacttg
    41041 cacgatcgat aaagaagact ttttatcaaa aggggagaat acacctttta tcggctggaa
    41101 agtaaaaggc gaaacacaaa tgacttttgt caacggaaaa cttgtgtggc agaagggaga
    41161 ataaaagtat ggaacgtttg cttatattag aagatggaac agttttcgaa ggaaaggctt
    41221 ttggtgcaga tgctagtgtc ttcggagaaa tcgtttttac aacaagtatg actggttatc
    41281 aagaaactat cactgatcaa agttttaacg gtcaaatcat tacatttacc tatcctatgg
    41341 tgggtaatta tggggtaaat agagatgatt atgaatcgat ttctccaaca tgcaaaggag
    41401 tagtagtaaa agaacatgca cgtgtagctt caaactggcg aaaccaaatg acgctagatt
    41461 catttttgaa acaaaaagga attcctggaa tatcaggtat cgatacgcgc gcccttactc
    41521 gcaaaatccg tgaagtcgga acgatgaaag ctagtatagt tgatgctgga gattccttcg
    41581 aacatgcatt tgatcagctg aaagcaaccg ttctagcaac caatcaagtg cagcaagtct
    41641 caactaacaa accttatcca agtccaggaa caggtcggaa agtcattgtc attgacttcg
    41701 gcttgaaaca tagtatttta cgagaattga gtaaaagaaa ctgtaacctc acagtacttc
    41761 cctataacac agatgccgag acgattttag cgctatcacc agacggagtc atgctgacga
    41821 atggacctgg tgatccaaaa gatgtgccag aagcaatcga aatgatcaaa aaaatccaag
    41881 gaaaagtacc gatttttggt atttgtctcg gtcaccagtt atttgcttta gccaacggtg
    41941 cagataccta taaaatgaaa ttcggacatc gaggattaaa tcacccggta cgagaagtag
    42001 cgaccggaag aattgatttt acttctcaaa atcatggcta tgcagtcgat gaagccacga
    42061 ttgatccaga aaaactcatc gtaactcatg tagaagtaaa tgacggaaca gtagaaggag
    42121 tcaaacacag agattatccc gctttcagtg tgcagtttca tccagatgct gcccctggtc
    42181 cgcatgatgc actacattta tttgatgaat ttatggaaat gatggacgca ggaaaggagc
    42241 agtaaacaga tgccgaaacg tacagatatc aagaaaatta tggtgattgg atctggtccg
    42301 atcatcattg gtcaagccgc agaatttgac tacgcaggaa cacaagcctg ccttgcctta
    42361 aaagaagaag gatatgaagt ggttttagta aattctaatc ctgcgacgat catgacggat
    42421 aaagaagtgg cagatcaagt atatatcgaa ccgatcacac ttgaatttgt atcaaggatt
    42481 cttcgaaaag agcgtccaga tgctctcctt ccaactttag gcggacagac aggcctcaat
    42541 atggcaatgg agctggcagc atcaggtatt ttagaagaat tgaacataga acttctagga
    42601 acaaagcttt ctgcgatcga tcaagcagaa gatcgtgatc tattcaaaca gttgatgaaa
    42661 gaattgaatc agccaatccc cgagtctgaa atcgtcaata cagtagaaga agctgtagat
    42721 tttgcaaata aaatcggcta tcctgtcatc gttcgtccag cctttacttt aggcggaact
    42781 ggtggaggta tgtgtgaaaa tgaagaagaa ttacgtatcg tatcagaaaa tggattaagt
    42841 ctttcccccg ctacccaatg tttgattgaa cgaagtattg caggttttaa agaaattgaa
    42901 tatgaagtga tgagagattc ggctgataat gcaatcgttg tctgcaatat ggaaaatttt
    42961 gacccggtgg gtatccatac aggtgattct atcgtatttg cccctagtca aacattgtct
    43021 gaccatgaat atcaaatgct tcgtgatgct tctctttcga ttattcgagc acttaagatc
    43081 gagggaggct gtaatgtcca actcgctctt gatccacata gttttaatta ttatgtgatt
    43141 gaagtcaatc cgcgtgtttc tcgttcatct gcgttagcaa gtaaggcgac tggctatccg
    43201 atcgctaagc tagctgctaa aatagcgctt ggattaacat tagacgaaat gaaaaatcct
    43261 gttacaggaa cgacttttgc agaatttgaa cctgcattag attatgttgt agctaaaatt
    43321 cctcgctggc cgtttgataa gtttgaaaac ggagaacggc ttttaggcac gcagatgaaa
    43381 gcaactggtg aagtgatggc tatcggtcgg aatatcgaag agtctttatt gaaagctgtg
    43441 cgttctttag aaatcggtac acaccatatc gaacttcctg agttggcaga catcacggac
    43501 gatgacctaa tccaaaaagt ggttcgagca caagatgatc gcctgtttta tgtagctgaa
    43561 gctattagaa gagggtacac catagaagaa ttagcggatt tgacaaaaat cgatttattc
    43621 tttttagata aactgttgca tatcgtagaa atcgaacagc agttgaaaca gcagccgttg
    43681 gatgacaaga tattgaaaac agcgaaacaa aacggtttta cagacaaaaa agtggcggag
    43741 ctgtgggaaa taaacgaaga agccgttcgc cagatgagaa aagaaaatga tttgattcct
    43801 gtatataaga tggttgacac ttgtgcagcg gaattcgaat caaaaacacc ttatttttat
    43861 agttcctatg aatatgaaaa tgaaagttca cgatcagaaa agccctcaat tctagtactg
    43921 ggatctggtc ctatccgtat cggacaaggg gttgaatttg actatgcgac tgtgcattct
    43981 gtcaaagcaa tccagcaggc aggatacgaa gcaatcatca tgaacagcaa tcctgaaaca
    44041 gtttcgactg acttttctat ttcggataaa ttatattttg agccattgac cttcgaagat
    44101 gtgatgaatg tgatcgagct agaacagccg ataggagtaa tcgttcaatt tggtggtcag
    44161 acagcaatca acttagccga acctttgact gctgctggtg taaaaatctt agggacaacg
    44221 atcgaagact tggatcgtgc ggaaaatcgt gatttattcg agcaagcgct gaaagagttg
    44281 gatattcctc aacctttagg ggatactgct acaagtaaag aagaagccgt ggttatcgct
    44341 tcgagaattg gttatccagt tcttgttcgt ccaagctatg tattaggcgg acgtgcgatg
    44401 gaaatcgttg aaaaccagcg agatttagaa gattacatgg aacatgctgt gaaagcatct
    44461 ccagaacatc cagtactagt ggaccgttac ttgcttggtg gtgaatgcga agtggatgcg
    44521 atttgtgacg gtgaaacagt actcatccca gggatcatgg agcatattga gcgtgctggt
    44581 gtccattcag gggattcaat ggctgtgtat ccacctcaaa cattatctga atcgatcaag
    44641 aaaacgatcg aagattacac gatccgctta gcattaggtt tgaattgtgt cgggatgatg
    44701 aatatccagt ttgtcatcca tgaaaatgag gtttatgtga tcgaagtcaa tcctcgagca
    44761 agtcggactg ttccattctt aagtaaggtg acaggaattc caatggcaca aattgcgacg
    44821 aaagctattt tgggagagaa actaaagaca cttggttatg aaaacggact ttatccagaa
    44881 acaaaagaag ttcatgttaa agcacctgtc ttttctttta cgaaattgca gaaagttgat
    44941 acttacttag ggcctgaaat gaaatcgact ggagaagtaa tgggatctga cagacaatta
    45001 gacaaagctt tgtacaaggc attcgaagct tctggattac acttgcctga atatggagcc
    45061 gtattattta cgattgcgga cgaaacaaaa gaagaagcac tagaattagc taaacgtttt
    45121 acagaaattg gctatagtct gataggaacg aaacatacag ctgcttattt cgaaaaaaat
    45181 ggtctggtcg ttgaaacagt agcgaaaatt tctgaggaag cagcagaaaa aaatgtagtc
    45241 gacttaataa gagatggtaa aacacaagtg gttgtcaata cgattgataa agatcgggga
    45301 aatgcttcta aagacggttt catcatccga agagaagcag ttgaacatgg tattccattg
    45361 tttacttctt tagacacagc agatgcgatc gttcgagtga tggaatcccg tgctttttca
    45421 actaacccta tttaacggcg agcacatgtt aagacaaaaa gacgaaaaac cgttgcagga
    45481 ggggacagat gaaacaagaa atgatggaaa tcgttcagca aaaacagctg gcaccaaaga
    45541 tttttgaaat gacattgaaa ggtgatctag taaaagaaat gcagcaacca ggacagttct
    45601 tgcatattct cgtgccgcgt gcagacttgc tattacgccg accgattagc ttgaatcata
    45661 tcgaccacga aacaaatact tgccgtatca tttatcgggt ggaaggtgag gggactagag
    45721 tcttctctac tctttctgcc ggagaccatt tagatgtcat gggtccttta ggcaatggtt
    45781 ttgatttgtc caatcttcat acaggagacg aagtatttat aatcggtgga ggaatcggtg
    45841 tccctcctct atatgagtta tcaaaacagt tagtcgaaaa aggaattaag ccaacgcatt
    45901 ttttaggttt tgccacacat gaagtcgttt attatgaaaa agaatttaat cgtctaggag
    45961 aaacaagaat cgctactgac gatggaacat acggtatgca cggaaatgtc ggtaatttat
    46021 tgttaggaac gaagacggaa cctacagctg tttttgcatg tggttcaaac gggttattga
    46081 agacagtgga acagctgttt tcttcgcatc aaaatgtcca gttatcatta gaatcccgca
    46141 tggcatgtgg gataggggct tgctatgctt gcgtttgtca tacgccggaa gatgaaacag
    46201 gaacaaaaag tgtgaaagtt tgtgacgaag gaccgatttt taaaatagga gaggtaatta
    46261 tatgaatgag aatcctttag cggtcaaact tcctggccta gaattaaaaa atcctatcat
    46321 gccggcaagc ggctgtttcg gttttggcaa agaatacgga aaatactatg atttgaatca
    46381 gcttggatcg atcatggtca aggcaacgac tccaaatgcc cgctttggca atcccacgcc
    46441 aagagtggcg gaaacaccat ctggcatgtt gaatgcaatt ggtttgcaaa atcctggtct
    46501 agacgttgtt atgcataaac ttcttccaga attagaggag tacaatgagt tacccatcat
    46561 cgcaaatgtt gctggtgctt gtgaagaaga ctatgtagaa gtttgtagca aaattggcga
    46621 agcaccaaat gtgaaagcaa tcgagctgaa catttcttgc ccaaatgtca aacatggcgg
    46681 aattgctttt ggaacagatt ctgatgttgc ttttcaatta acacaagcag taaagaaagt
    46741 ttcttctgtt cctgtctatg tcaaattgtc cccaaatgta acagacatcg ttccaatcgc
    46801 tcaggcaatc gaagctggag gggcagatgg gtttacgatg atcaatactc ttcttggtat
    46861 gcggattgac cttaagacaa gaaaaccgat tttagccaat caaacaggtg gactctccgg
    46921 accagcaatc aagcctgtcg ctattcgttt gattcaccaa gtagcaagta tatccagcct
    46981 tccaatcatt gggatgggcg gcgtgcagac agtggatgat gttcttgaga tgtttatggc
    47041 tggagcaagt gctgtagcta tagggaccgc gaatttcacc gatccgtata tctgtcctaa
    47101 actaattaaa gagctccctg tgcgaatgag cgaactaggc atcgaatcgc tggaaagatt
    47161 aatcaaagaa gtgagagagg aacgtgaacg atgaatcaac tagaccaacg accaattatt
    47221 gcattagatt tttcaactag gcaagaggta gaagactttc ttcgattttt cccgaaagaa
    47281 gaaaaattgt ttgtgaaaat cggaatggaa ttgttctacc aagaaggacc tgaaatcgta
    47341 cgttatctaa aagaggctgg tcataatgta tttttagatc tgaaacttca cgatattccg
    47401 aatacggtcg aaaaagcaat gcggggattg gcaaagcttg gtgttgatct cacctgtgtt
    47461 catgccgctg gcggtattcg tatgatggag gcggcaatgc gaggattaga agaggggact
    47521 ccagaaggtg gaaaacgtcc gctacttcta gctatcactc agcttacttc tacaagtgaa
    47581 gaagaaatgc atgccgatca actaatcgaa gtttctttag aaaaaagtgt gatccattat
    47641 gctagctgtg ccaaaaaagc aggattagat ggtgtggttt cttctgcttg ggaagtagaa
    47701 gcgatcaaag aaatggcagg agacgaattt gtttgcttaa ctccgggtat aagaccagaa
    47761 ggaacagtcg caggagatca gacaagagta gtcacacctt ctcaggcaaa aaagatcggc
    47821 tcaactttta tcgttatagg tcgaccaatc actcagtcaa ctaatcctta taaagcatat
    47881 caaacgattc aaacagaatg gagctaacct aaaatgaatg ttgaacaaag tatcgcaaaa
    47941 gacttattag aaattgaagc agtattttta aatccaagta atccttttac atgggcaagt
    48001 ggaatcaaaa gcccaattta ctgtgataac cgaatcacga tgagctatcc aaaagtccga
    48061 aaagagatcg caaaaggatt agcaagcaaa atcaaagaag cttaccctga agtacaagtg
    48121 attgctggaa ctgcaacagc aggaatccct catgccgcat gggtagcgga aatactggat
    48181 ttgccaatgg tctatattcg aagtaaggcg aaagatcatg ggaaaggtaa ccaaatcgaa
    48241 ggacgtatat ttgaaggtca gaagatggtt gtgatcgagg atttgatttc tacaggcgga
    48301 agtgtcttgg aagcagctga agcagcaaaa agagaaggtg cggatatcct tggtgttgcg
    48361 gctatcttta cgtatgagtt gccaaaagga aaagcaaatt tcgaaaaagc cgaaatccct
    48421 cttatgacgt tgaccaatta ttctgtattg attgaagcag ctttagaaga tcgttatatc
    48481 gatgagcaag aattaactct gctaaaagaa tggaaaaaag atcctgaaaa ttggcagatt
    48541 gaagcgcgct tgatgaagat agagaaatcc gatttgtgtc tccagaatca tcaattggaa
    48601 tagcaaaaat ccaaaaactg attgcgagaa gcatcttttc atcaggtatg aaaagaaact
    48661 tctatcgtga tcagtttttg atttttttaa aattcatttt tctaagactg gttagcagaa
    48721 agaagagaat aatctcttct cttttttcta ttatatatag attttctcta ttttttggta
    48781 agggagctgt ttcaaaaatg gtaaagagcg gaaatgttgc gaatatcagt tatttaatga
    48841 tatgatattc ggaaaaagaa atctcaaagg gaggtaactt ttatggggtt gaatgaattt
    48901 attttaggat tgaacaattt agagacgatc acaagggcac ctggattttt taaatataca
    48961 gagcatacag ttgcagcaca ttcgtatcgt gtagcatcta ttgcgcaagt tctaggagac
    49021 attgaagaag cgaatggaca accgatcgat tggaaactgc tctatgaaaa atcattgaat
    49081 catgactaca cagagcgttt tattggtgat atcaaaacgc cggtgaaata cgcaagcaaa
    49141 gagttaagag ggatgctgca aacagttgaa gaaaagatga cggacgaatt catcaaccaa
    49201 gagattccag ctgagttcca agaagtttat cgccgtcggt tgttcgaagg aaaagatgaa
    49261 accgtagaag gagaactatt agccatttca gataaggttg accttcttta tgaatctttt
    49321 gatgaaatca tcaaaaataa cccggaaaaa gtttataagg agatgttcat tgaggcagtg
    49381 actacgatta aaagctttga acatcggcct tctgttcagt atttctttaa agagatattt
    49441 ccggaactat tgaaccagga attttatgga aaagaggcgt ttttagaaac cgtctcaacg
    49501 attgtaaaga aagaagaaaa ataaaaatga tatgagatgc agggggaagt atagttgaaa
    49561 aagaatatgg atgtcgaatg gggctattct ggagacaaag gtccggaaaa ttgggcgagt
    49621 ttatgtgatt ggtttgcgag aggtgcggaa tttccattgc agtcgcctat tcatttgacc
    49681 aagggggaag aaaccaaaat agaaggagac acgctttcct ttcattatca aaagcaacgg
    49741 tttactgaca aggaattcaa aaacacgatt catttagtac cttttgacca actcagttat
    49801 gtagagttca agaaagaacg ctattatttg acagatattc atttccactt gccttctgaa
    49861 catatcatca acggaaaaca ggaagatatt gaatttcatt ttgttcatat gaacagcaaa
    49921 aaagagaatc tagttgtggg tgtcatgtac cagctaatgg tacaggaagg ttggctgtat
    49981 aatcaagaaa atggggaaag ttggaactta gaaaaacatg agcattgggt aaatccggat
    50041 gtgttctttc cggagcaaaa aagccatttc cattatattg gctcattgac aactccgcct
    50101 accagcgggc caatccaatg gtttgtaatg gatcatgtag gaaaactgaa ccaagagttt
    50161 cttctcccct tcgatggtca gtatgctcga ccgaacaacc gaccaatcca gcccttgaat
    50221 ggaagagaaa tttattattt cgaggagttt gagcaatgaa tattcagcag atgaaatacg
    50281 tggtcgctgt agcaaacaat ggtagtttca gagaagcggc aaagaaaatg tttgtctccc
    50341 agccaagtct ttcagctggt atcaaagaat tagaaaccga gttagggatc acgctgttta
    50401 ctcgaacgaa tcgaggggca tatttaacag aagaagggca agacttctta aatcgtgccg
    50461 agaaaatatt ggtccagctt gatacattag aaactcacta tctaacgagt gaaaagagag
    50521 acaatttttc gatcgctgcc cagcattatg atttcttagg tcctttgacc gcacagctac
    50581 tcgaggaatt caaagacgag atcaaacaat ttcggatcat ggagacgaca acagcgagag
    50641 tgatcgaaga agtcaaagag cagcacagtg agatcggcat tatttatatg aatgagcata
    50701 atcaagctgg tatcagaaga tatttacaac aaggagagct aagtggccgc agcattggaa
    50761 aattcaaaac gcatattttt ttaaggcaag gacatccatt agctgaacac gcagagatca
    50821 ccaaagaaga attgttatct tatcctcaag ttcgttttac acaggatgga aataatttcc
    50881 cgtattttta tgaagacctt atcgagatcc ctgatcaaga aacagtgatt tatacgagtg
    50941 atcgaggtac tttgatgaat atcgttcttg aaactgatgc ttacgcttca ggatctggta
    51001 ttgtgattgg agggattcgc gagcacttgc ggttaatccc tttggcagaa ggcgaactaa
    51061 atgaatttta tatcatttat tcggcgaaac gtccgctcag tgaaatcgct caaagattta
    51121 tcaaagagct tactcggtta ctggaccagc agagctaaga aaagcagatg agaacgctgt
    51181 ttattgaaca ccgttgatca aaaaagaggg gttgtgacag aattttgtca caactcctct
    51241 ttttgtagca aacaatttat aactaattgc tgttgtggga aagtacgcaa atatgattct
    51301 agaaacactc attgattacc tgttttgttt catagctgta attttttctt cttctggtgt
    51361 cacgataatc gtcttttggt tttcataaat aacataacca ggcttggcgc catttggttt
    51421 tcggatatgc tttacctgta caagatcaac cggcacttgg gcagagtatc ggtattttga
    51481 aaaataggca gctaactcgg cagcttctgt aattgtttca tctgagggct tgtcactttt
    51541 aatgatgacg tgcgaaccgg gtatattttt tgcatgaagc caatagtcgg tttttttagc
    51601 tgtcttcatt gtcagctggt cattttgaag attgttttta ccaacaagaa tgagcgttcc
    51661 gtcagaagaa aagtattgat caggctgaga tggtttctta cgtttttgtt tttttaagtt
    51721 cttctttttc aggtatcctt ctgaagtcaa ttcttcttta attgcttcga tatccattgg
    51781 gccggcaatt tccagttgag ataagacaga ttcaagatat tggatttcat ctttggcttc
    51841 ttggatttgt tcgccaatca gcttaacagc gttcttcagc ttttggtatc gatggaaata
    51901 tttctgtgca ttttgatttg gtgtcaatgc cggatctagt gctattttga ttggccgatc
    51961 ttcttcataa taattaggta aaacaacttc ttttgcgccg cgtggtactt gtgtcagaaa
    52021 agtagtaagt agttcgccat ttctacggta attctcagcg ttttcggatt ctttgagtgt
    52081 ttgttcacgt tttttcaatt tatttttatt tcgttttagt tcattttcga tttttcgaat
    52141 cagttctccg ccttgttgtt ttgcccgatc tttctctgct ttttcgtgat aatagctatc
    52201 taataattga ctgagtgttg gataagctgt tacagcagat gcctgttcgg acaatacctg
    52261 ataagcaatc ggtgtgaaaa aatctttatt ttccgtttcg taaaaagtag gaataggctg
    52321 atggctgatg gctgagaaaa attcgtccca tacaaccatc ttttcattag gacgttccag
    52381 taatcttttg gtcagctctt gagcgatatc gaaaccgatt ccctgaaact gacgttggat
    52441 tgcttttgga tctagttcag tttgggaaag ccgctgaaag attttttctt tctcggagct
    52501 aaatgggttc aagacctctt gttttggcgg agcaacatat tctacaccag gaagcaaaga
    52561 acgataagtg ttttgagagc ttccaatgtg tttgatcgca tccagtattt ttccagtttc
    52621 tcgattaact aagacgatcg tactatgacg tcccattaat tcaacgatca atatgatatt
    52681 ttgtagatca cccaactcgt ttcgtttagc aaagtggaaa tggatcactc gatcattttc
    52741 gatttgttcg atgtcttcta aaatagcgcc atccaaatat tttcgaagca tcatcacgaa
    52801 gttaggcgga ttatcaggat tctgataatc gatttgggta atctgtacac gagcataact
    52861 tgggtgcgca gataataata atcgatggtt tttgccgcgt gaacgaatca ccagcacgat
    52921 ttcattttca taaggctgat ggattttcga gatccggcca gatagtaacg tttcacgcaa
    52981 ttcgttgacc atcgcgtggg taaatacacc atcaaaggac atggttcatt cctcatttct
    53041 tttcataagt atattcatta taacgaaaaa aagaagaaaa accaatttgg ccgaatagta
    53101 aagcaaaagt ctagaaaaat cttggactaa ttacataaaa aacagtgttt ctatcgttga
    53161 aatccaaatt tttaggt
//
LOCUS       DARBDV010000008        50270 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000008 DARBDV010000000
VERSION     DARBDV010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.008"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..286
                     /locus_tag="SCM56_000485"
     CDS             <1..286
                     /locus_tag="SCM56_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEG1109313.1"
                     /translation="NVSVTESAPVNIEIVPQNVSKGVVFDRLESIYNLKKPLRIAIGD
                     SLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFERILTI"
     gene            567..1787
                     /locus_tag="SCM56_000486"
     CDS             567..1787
                     /locus_tag="SCM56_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HEG1109314.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            1784..4504
                     /locus_tag="SCM56_000487"
     CDS             1784..4504
                     /locus_tag="SCM56_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1109315.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            4488..5432
                     /locus_tag="SCM56_000488"
     CDS             4488..5432
                     /locus_tag="SCM56_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEG1109316.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(5501..6511)
                     /locus_tag="SCM56_000489"
     CDS             complement(5501..6511)
                     /locus_tag="SCM56_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEG1109317.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(6691..7035)
                     /locus_tag="SCM56_000490"
     CDS             complement(6691..7035)
                     /locus_tag="SCM56_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEG1109318.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7028..7462)
                     /locus_tag="SCM56_000491"
     CDS             complement(7028..7462)
                     /locus_tag="SCM56_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEG1109319.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            7600..8337
                     /locus_tag="SCM56_000492"
     CDS             7600..8337
                     /locus_tag="SCM56_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1109320.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            8330..9541
                     /locus_tag="SCM56_000493"
     CDS             8330..9541
                     /locus_tag="SCM56_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1109321.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            9745..10527
                     /locus_tag="SCM56_000494"
     CDS             9745..10527
                     /locus_tag="SCM56_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEG1109322.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            10597..11259
                     /gene="trmB"
                     /locus_tag="SCM56_000495"
     CDS             10597..11259
                     /gene="trmB"
                     /locus_tag="SCM56_000495"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEG1109323.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(11357..13111)
                     /gene="ade"
                     /locus_tag="SCM56_000496"
     CDS             complement(11357..13111)
                     /gene="ade"
                     /locus_tag="SCM56_000496"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HEG1109324.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            13243..14172
                     /gene="tadA"
                     /locus_tag="SCM56_000497"
     CDS             13243..14172
                     /gene="tadA"
                     /locus_tag="SCM56_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HEG1109325.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14129..15178
                     /locus_tag="SCM56_000498"
     CDS             14129..15178
                     /locus_tag="SCM56_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HEG1109326.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            15175..15453
                     /locus_tag="SCM56_000499"
     CDS             15175..15453
                     /locus_tag="SCM56_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEG1109327.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            15428..15898
                     /locus_tag="SCM56_000500"
     CDS             15428..15898
                     /locus_tag="SCM56_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEG1109328.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            15915..16217
                     /locus_tag="SCM56_000501"
     CDS             15915..16217
                     /locus_tag="SCM56_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109329.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            16234..16605
                     /locus_tag="SCM56_000502"
     CDS             16234..16605
                     /locus_tag="SCM56_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HEG1109330.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            16577..16936
                     /locus_tag="SCM56_000503"
     CDS             16577..16936
                     /locus_tag="SCM56_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109331.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17108..18115
                     /locus_tag="SCM56_000504"
     CDS             17108..18115
                     /locus_tag="SCM56_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG1109332.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18140..19324
                     /locus_tag="SCM56_000505"
     CDS             18140..19324
                     /locus_tag="SCM56_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEG1109333.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(19761..21344)
                     /locus_tag="SCM56_000506"
     CDS             complement(19761..21344)
                     /locus_tag="SCM56_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEG1109334.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(21922..22065)
                     /locus_tag="SCM56_000507"
     CDS             complement(21922..22065)
                     /locus_tag="SCM56_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HEG1109335.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(22287..22766)
                     /locus_tag="SCM56_000508"
     CDS             complement(22287..22766)
                     /locus_tag="SCM56_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEG1109336.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(22839..23123)
                     /locus_tag="SCM56_000509"
     CDS             complement(22839..23123)
                     /locus_tag="SCM56_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109337.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(23201..23395)
                     /gene="ssrS"
                     /locus_tag="SCM56_000510"
     ncRNA           complement(23201..23395)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="SCM56_000510"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(23467..24753)
                     /locus_tag="SCM56_000511"
     CDS             complement(23467..24753)
                     /locus_tag="SCM56_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEG1109338.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            24891..25562
                     /locus_tag="SCM56_000512"
     CDS             24891..25562
                     /locus_tag="SCM56_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEG1109339.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            25528..26016
                     /locus_tag="SCM56_000513"
     CDS             25528..26016
                     /locus_tag="SCM56_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEG1109340.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26029..26976
                     /gene="prmA"
                     /locus_tag="SCM56_000514"
     CDS             26029..26976
                     /gene="prmA"
                     /locus_tag="SCM56_000514"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEG1109341.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            26977..27741
                     /locus_tag="SCM56_000515"
     CDS             26977..27741
                     /locus_tag="SCM56_000515"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEG1109342.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            27974..30187
                     /locus_tag="SCM56_000516"
     CDS             27974..30187
                     /locus_tag="SCM56_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEG1109343.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            30198..30644
                     /locus_tag="SCM56_000517"
     CDS             30198..30644
                     /locus_tag="SCM56_000517"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEG1109344.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            30815..32074
                     /locus_tag="SCM56_000518"
     CDS             30815..32074
                     /locus_tag="SCM56_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEG1109345.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(32163..33110)
                     /locus_tag="SCM56_000519"
     CDS             complement(32163..33110)
                     /locus_tag="SCM56_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEG1109346.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            33504..34796
                     /locus_tag="SCM56_000520"
     CDS             33504..34796
                     /locus_tag="SCM56_000520"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEG1109347.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            34811..36580
                     /gene="aspS"
                     /locus_tag="SCM56_000521"
     CDS             34811..36580
                     /gene="aspS"
                     /locus_tag="SCM56_000521"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEG1109348.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            36689..37564
                     /locus_tag="SCM56_000522"
     CDS             36689..37564
                     /locus_tag="SCM56_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG1109349.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            37830..39713
                     /locus_tag="SCM56_000523"
     CDS             37830..39713
                     /locus_tag="SCM56_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEG1109350.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(39788..40072)
                     /locus_tag="SCM56_000524"
     CDS             complement(39788..40072)
                     /locus_tag="SCM56_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEG1109351.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40062..40796)
                     /locus_tag="SCM56_000525"
     CDS             complement(40062..40796)
                     /locus_tag="SCM56_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEG1109352.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            40925..41548
                     /locus_tag="SCM56_000526"
     CDS             40925..41548
                     /locus_tag="SCM56_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEG1109353.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            41740..42861
                     /locus_tag="SCM56_000527"
     CDS             41740..42861
                     /locus_tag="SCM56_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HEG1109354.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            42868..45996
                     /locus_tag="SCM56_000528"
     CDS             42868..45996
                     /locus_tag="SCM56_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HEG1109355.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46015..49209
                     /locus_tag="SCM56_000529"
     CDS             46015..49209
                     /locus_tag="SCM56_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HEG1109356.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(49355..50062)
                     /locus_tag="SCM56_000530"
     CDS             complement(49355..50062)
                     /locus_tag="SCM56_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEG1109357.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16409 a         8837 c        10487 g        14537 t
ORIGIN      
        1 aaatgtgtct gttactgaat cggcacctgt aaatattgaa atcgtacctc aaaatgtatc
       61 taaaggtgtg gtgtttgatc gtttggaatc aatctacaat ttaaagaaac ctttgagaat
      121 cgcgatcgga gatagtttaa atgacttatc tatgtttgaa agtgcagata ttagttttgc
      181 aatgggaaat agtcatcaag aaatcatgaa aaaagctgat tatgtcactg tttcaaatga
      241 taaagacgga gtatctcaag catttgaaag aattcttacc atataaacta attaaggtag
      301 tatagaacat agaattctaa atacggaaaa atggagcata aattccagta caaatagtac
      361 ttcttcagtg tcaaacgaag cgaatataac atagatattt aggattgtga caaaaatcat
      421 gtcacaatct ttttttcgaa taaacttatt ttttgaagtc ggccatttat cgtaatttct
      481 ttgcataata cgaacaaatt ttcggttatt gatgaaaaaa aacattgaaa atggtatgat
      541 aaaagaaaaa aaggaggaaa tttttaatgg tgaaatttat ccatgcggca gatttacata
      601 tggatcggtc atttgaagga ctgacaactc ttgataaaac tgtacaggaa aaactattga
      661 agacgaatct gaccgtttta tcaaatatca ttgaacaggc aataataaat aacgttgatt
      721 ttgttttact tgcaggagat aattttcatc aaaatcgtcc ttcattaaaa atacagaaac
      781 atttttcaga acagatgaaa cggttagaaa agaaccatat acctgtattt ataatattcg
      841 gaaatcatga tttctatcaa aaagagcggt attggttctc ctttcctaaa aatgtccatt
      901 tatttacatc cgaaacggta gagacaaaaa aaataacgat aaaaagtgga gaaactgtct
      961 ctctcagtgg atttagttat cgtcaaccgt ggattcaaaa agacaaagtg atggaatttc
     1021 cttctagaga actcacagat taccatattg ggttgtacca cggggaacct ggcgtctctc
     1081 aaaaaggaaa ctatgctcct ttccaaccct caaagatgca agaaaaggga tacgattatt
     1141 gggctttagg gcatatccat gtgccaacgg tcttaaatga aaagcaaaca attgtttatc
     1201 caggtgcgcc tcaaggtcat actaaaaagg aacaagcttc tacatctatt ctactagttg
     1261 aattaagtaa aggaagttgc caaatacatc cgattaaagt agcagaggtc tattggaaga
     1321 caaaggaaat atcattgagg aaagcaagaa cgacaaaaga ggtcattgtt cacattcgtc
     1381 agcagctttc aaaagtagag gatggattta gtttaattga gataaaatta aaagattatg
     1441 accatctcaa tacagatgtt ttggaacgga tacaatcagg agagctatta gactatttgt
     1501 tagaggaatt ctcagatagg ataaatgatt tctttatttg gcgtatttct ctcatagaga
     1561 atacttttta tacgaagacc ccacttgctg cttctgctaa attgatggaa cagttatttc
     1621 aatactatca gacacctcaa gatttccaac aaattttaaa tgaagtatat agtcatcaag
     1681 aagcggctag aatattgagc gaactgccgg aatatcaaga agaaacactt gaaaaagcca
     1741 agcagctgct taatcaagat tttttatttg aggaggatca ggaatgaaga ttttaaggat
     1801 cgagattgct gcttttggaa aatggcgtca aaaaagtttt gacttttatt caggcaatca
     1861 gctgatctat gggggaaacg aagcaggaaa atcaacgatt tatcaattta ttcaagcaat
     1921 attatttggt ttcccttcaa aaggccgtaa aaaaaaggat tatacaccaa aagacggctc
     1981 agcctatggc ggcaaaatat ggcttaaaca tcctgtatat ggtgaatttg ctgtcgagcg
     2041 ctacaagcag caaaatcgcg gaaaatcgaa ggtgtggctg ggggatcaag tgggaagtga
     2101 tgagttacta gaaaaactga tcgcaccttt gacgaaagag ttattccagc aggtatttac
     2161 tttccagcaa gagcagttga tggaactaga tcatttgaga gaaaaagagc tacatgatgc
     2221 gctgatttct ttaggcatca cgggcagttc tcaagtattc caaaagcggc aagaatatta
     2281 tcagaaagca caacaattat tcaagaaaaa aggccaaaaa ttactaatca atcaaaaatt
     2341 aaatgaatgg aaagagctgc aagaaaaaat tcatcaaaaa caagaacagg aagcttattt
     2401 tggcaagttg atccaacaag aacaggaata cgagtcgcaa cagcagcagt tattggcaca
     2461 attaaaagag gcgaacaaac aattttcatt agccagacag caagaactta atttttcttt
     2521 atatgaagaa tggcaatctc tcaaaaaaaa cagcactaca ggtcttcctg atgagcaact
     2581 tcttttagat ctggagtctt tctccaaacg gtatcagcaa acaaatgagc gtttggaaga
     2641 attagaagca acactggaaa agcatagcgg gatggatcag cagtcagcca gatattattt
     2701 ttatttagag caggagcagg aactaaaaga tctccagcaa cttcaattta cagcggaaca
     2761 attaaacgaa gaatggcaaa gattacaaat tgagactaga gaaacaaatt cagaattgaa
     2821 gattttggaa caaaggtggc attggcagaa aaatccgtta ccggaagaat ttttatatga
     2881 agaagaatgg aagcatcttc tggctgaaga aagaaggatt gctgatctag aaagccagat
     2941 ttctgtgcgt ctccaaatag ctaaagaaca gcaagagatg attgaagcag aagtgaatga
     3001 cttggaagag acctatccag cattattaca aaatcagtca gagatagcac cagtctcttc
     3061 atctaaaccg tatatgctga tggctggcgg agcaataatc attgcaggta tatttgtacc
     3121 aatgcccttg aaagtgatcc ttttactaat tggtgcaggc atgattggct atttcttcta
     3181 caaaaggcaa ccaaaaatta aaactaaaaa tgatttctct gagataaaag gaatgtggca
     3241 agaaaaacta ggacaacttg atctagcctt agctcaatta gctgaagcag aagaaaaaca
     3301 aaagagaact ggagaagaaa aacagattct tcagcaagaa gtatatcgtt ttgcggatca
     3361 tcggcactta ggaaagatgg atacgctcaa actcatgaaa gaatatggac aggaagtcat
     3421 agattatcag aaattatatg gcgtatttga gcggaaacat aaaagagaac aggaaataca
     3481 agaaaaacta cgacaaatcg accaaaaatt tgagtttgtt aaagaatggt taccgataca
     3541 agataagaca atttcagaaa aaatggtatt gctttcccgt tttatacaag agatggaaca
     3601 gatgaaattt gccagaagct atcagcaaaa tactttattg aagcaagaaa tcaatcaatt
     3661 gaaaaagaaa caaaaagtat ggatacaaga atacagcgaa caattgactc aagcaggctt
     3721 atcttatcct tcggaagtac ctgtcttttt acaaaaagcc aaacaagccg aacagcagat
     3781 gaaaagatac aatgaattat cggaaatgct tactccacta tttcctgaag agctgacaaa
     3841 agagaaatta tctgtaagac tgcgccaact gaaagaaaag caatcccaac tccagcaaga
     3901 agaaaaagaa gcaagtgaaa atcggcaacg actccaactt caagtagaag agttgcaaag
     3961 agacggcaca ttggacgaac tttatcaaca agctagccgg caaaaagcaa aattacagga
     4021 attactggag gaatggggag cacttgaaat cgccgggaca ttcttaggtg atctttcaac
     4081 agagatgagt gaaagacaat taccccagtt gttaagagca gcaggtgatt atttaggtat
     4141 tttaacaaaa ggaaattatc agaagatatt actgaaaaat gatacactga ctttaacaga
     4201 cggatataca gaatttccca tctatgagtt atctacaggt acgaaagatc agctgattat
     4261 ggcgatgaga tttgcttacc tctcattaga agctaaccgt tcgatatgcc cgatcatcat
     4321 tgatgatggc tggcttcatt atgatcatca gcgaaagtat caattagctg aacttttagc
     4381 taaatttgga gaaaagtatc aaataatttg tttttcttct gataaagaaa tggtaagcta
     4441 ttaccatgag atgaaccaaa tcgtaaaaca attagaagga gcaaataatg aaaaaaattc
     4501 gtgatttagt aatcgatgag cagtttgaac aatttgtcct gatcaaaagt gcagatgtaa
     4561 gaatcgctaa aaacggaaaa aaatttattg cttttacttt ccaagatact tcaggtacta
     4621 tcgatggtaa atactgggat gcttcggagg aggaaatcaa taaatttaca gccggcacag
     4681 tcgtttttct aaacggtaaa agagaggttt atcaaggaaa ccctcaagta aagattcttc
     4741 atatgcgctt aacaagggta gatgagccag gagagcctag tttatacatg gaaagagccc
     4801 ctttgaagaa agaagaaatg gaagaagaaa tcaatcaatt gttgtttgag atcaccaatg
     4861 cgcattggaa tcgtattgtt cgctatctac ttaataaata tcaaaaacaa ttttttgagt
     4921 ttccggcagc aaaaagaaac catcatgcat ttgccagtgg attagcttat catactttga
     4981 caatgcttca cttagggaaa tcaatcgccg atgaatatcc tgagctgaat cgagcattgt
     5041 tgtatgctgg tatcattttg catgatttag gaaaggtgat tgaactttcc ggtcctatgt
     5101 cgacagaata tacgcttgca ggaaatttga tcggtcatct ggtattgatt gacgaagaaa
     5161 taactaaagc ctgtcttgcg ttgaaaatca gcgaagctga cgaagatgtt cttgtattac
     5221 gtcatatggt tttagcacac catggcttat tagaatatgg ctcacctgta cgtccaagaa
     5281 taatggaagc agaaatatta catcaaatcg acaatatcga tgcttcgatg cagatgatgt
     5341 tgacttcgat tcgtcaaaca gaaccaggaa aatacaccga tcggattttc ggtatggaca
     5401 accgaagttt ctatgtacca aaagatttat agcaaaaatt tatttttttc attcaaaaca
     5461 aaaaaagcga gacataagtc ttcgcttttt ttgttttctt ttattttgat gaatcagttg
     5521 tttcttcagt tgaagattct tttgttgaat cagttgattt tgtgtcagaa ctctttgttg
     5581 aagctgtagt ttcacttgag tctttcgtac ttgatgaagt tgttgtgaat gcactcaaca
     5641 cattttcaaa ggcatcatct ttgattttta catttgcatc ttttaattct tcaccgatta
     5701 ctttagttgt gaatgcattg tcacttagtt tagtttcagt agcgatatct tttagttgat
     5761 ctttgtattt atccatgtca ttgcctttat tttggttttt caccattttg acaacgtagt
     5821 attctgtcgc ataagaagta ggatttgttg tagtaataac atcggagatc tcaccgtctt
     5881 tcaatttgaa agctgcttct tttacttctg caggaatagt tgttgtcgtt gagtcgaatt
     5941 taactttacc gccatcttct tttgtctctg tatcagttga tttgtctttg gctaatttag
     6001 agaaatcatc tccatcatcc gctgattttt tcacatcttt tgcttcatct tcacttgata
     6061 atttgatgat ttgtgcttct acttctggat ggaaagattt ccaagcagtt ttcaaatcgt
     6121 catctgtaat atctacgtga gctttcaagc cagcttcaaa agctaaattg ttacgaatat
     6181 attctttata agtatctttc gtatatccag cagcttctaa ttgtgattca aaagtatcac
     6241 ctaatgattt cgcttgtttg tcgtattctg catccacttg cttatcggtt actttatcgc
     6301 catatttgtt attgaatact ttataaatga tcatccgttg tactaaggat tggttcgatg
     6361 attcaagttt tgcttcatca tagaaatcag atacagtgat cgttccgcct ttcatcgttg
     6421 cgatatcttt attagtatcg cctgaacaag ctgctagagt taaaacagcc aacgcactag
     6481 tagctgccaa aataattgat ttttttttca ttaaaatttg cactcctgta attagttatt
     6541 atttttatat attaactata ccatatccca tttcctttaa aaacgttgtc gtggtagttt
     6601 caaacaaaat tcaaaatttc atcacattcc taggaaatac gcaaactttt ctcacttata
     6661 tagttaaatc tgttgagaaa ataatcatta ttatatttgt tcaggatact ctggcagatc
     6721 agcttttagt ttttctactt gttcattgat ttgtgtgata cgtggttcgg cttggaattt
     6781 aaaatcttcg atatcttttt tgattgcttc ttggaaagca ggaatcacag attcagctgt
     6841 ttcttttgtt tcgatcaatg cgcttttaaa atgattcagg ctgttattta aatcatttgt
     6901 cagagttgtt gcttcttcca attcttcgat cagtttcttt cttgtttcat tcccgctcct
     6961 gggcgccaat aacagacctc cgacagtgcc agcagcagct ccaaataaca gtcctttgaa
     7021 aaagttttta atcatcgcag gacacctgct ttacgattgt ttcttgaata gcttttagtt
     7081 cttccgatcc ataactatct tgatgattga caaagtgaat agaaaaatca tcctctttgc
     7141 tgtagcgagg gactaaatgg atatgtgaat gaaaaacaga ttgataggct agttcacggt
     7201 tgttattcaa gatattaaga cctttcattt ctggaaaagc tttttccaat gcttgagcaa
     7261 tcttaggcac acgcgaaaaa acctctcctg caagttcggg atcatattca aaaatatctg
     7321 ctacatgacg tttaggcaca actaatgtgt gtcctttcgt tgcttgagag atgtctaaaa
     7381 aagcatagac cttgtcgtct tcatatattt tatagcttgg gatttcttga ttgatgattt
     7441 tgcaaaaaat acagttttcc atataaaaca tcctttctat aaagaataaa tacttcagct
     7501 taactttacc ataagttaaa gctattgtaa ggaaaaaaac aaaataccgt cattttcttc
     7561 gtggtataat aaataaaaat aaatggcgga ggaaaaagaa tgagtttaat tatcgatcat
     7621 gtcacagggg ggtacggaca tctccctgtg ttaaaagata tcagtttcga tgtacagcct
     7681 ggagagatag tagggttgat tggactgaac ggagcaggga aaagtacgac catcaagcac
     7741 attatcggtc tgctggaagc ttcaaaagga acgatagaaa tcaatcatct atccttaaag
     7801 aaaaatacgg aagaatatcg caaacaaatt gggtatattc cagagactcc ttctttatat
     7861 gaagaattga cattgagaga acatatcgaa gtcactgcga tggcctacga tatacctatg
     7921 gatatagcaa tgaaaagagc agaaaagttg ttggagactt ttcggttatc taatcgtctt
     7981 gactggttcc cagctaattt ttcaaaggga atgaaacaga aagtgatgat cttatgcgcc
     8041 tttttgatcg agccttcttt atatatcatc gacgaaccat ttttaggctt ggatccacta
     8101 gctatcaatg cattgcttga attgatggta gaaatgaaaa aagaaggagc cggtattttg
     8161 atgtctacgc atattttagc tactgcagaa aaatattgtg atcgttttgt ggtcttacat
     8221 aacggagaga tacgagcaga cggttctctt gagatgttga gaaaagaatt tcagctgccg
     8281 gattcttctt tggacgaaat ctatattgct ttaacaaagg agcaggcaca tgagtagttt
     8341 ttttcaaaaa cgtttagcaa gacatcaaaa gaaaatgatg aaatatatgc gttatgtatt
     8401 gaacgatcat tttgttttag tttgtctttt cttgattggc ggtgtgggat tatattattc
     8461 taattggttg aaaaccctgc agccaccgtt tgcaattgga gggctcataa tctctgtatt
     8521 ttggatgctc tgtttattcg tgggtaagat ggctactttt gcagaggcag cagatattgt
     8581 ttttttactt cctaaagaaa aagaaatgca aacctatttg gaacgagctt ttcgctattc
     8641 ttgcattttt ccgttgatct ttttgatatt ggcatgcgga ttggccatgc cattagtggt
     8701 ggttagtacg gagcaatcct tttcgatgtt tttcgtctat ctgatcatat tatggagttt
     8761 gaaattcagt catctgcaaa tcaagagagc agaattgttt cgactagatc aacaagttat
     8821 tcgttattgg aaattaggct ggtttttttc ttctctgatc tttttgtttc tatcatttta
     8881 ttggctaata tgggcaggaa tagcaggtgc attagtacag gcaagtctat attactggtt
     8941 tttgtggaag caacaagaaa aaggattgga ttgggagaaa atggtgcatg tagaagaaca
     9001 taggttgcat cagatctatc aatttatcaa tttattcaca gatgttcctg aaattacggc
     9061 aaagatcaaa agaagacgct atttggatcc tatattgact catattaaaa gagaatcaaa
     9121 aaacacttat ctttacttgt ttgcaagaag attggcaaga ggttctgatt ttagtggtct
     9181 ttatttccga ttagtaatta taggtgggtt attgattgcc ggagtagtcg acttttattt
     9241 tagtctgatt atcggtttct tatttttata tttgatcggt ttccaactgt tacctttagc
     9301 gaaccagttc cgctatatgg ttctcgttca gctttatcca atccataaga atcagaagaa
     9361 gcaggcgatt cagtatctac tcacatgggt attaattatc gcgtcggtta tttttggttt
     9421 gattgctgca gtcgtattag atgggcagca gcgatgggtt ccgctagcgg cttatttgat
     9481 tgttactatg ctctttgtta aactatatgt accaactcgt ttgaagaaga tgtcagactg
     9541 aaaacaaagc tgtgtagggt aaaaatgaga atgaaaaata gagcgttgac agtaagaatg
     9601 ctgttcgttc tatttttttt gagaaaagct ggttttttga atggcaatta aatgaaaaag
     9661 aacgtatatg tgcttgacgt gtagtgttca ggcgactaga ataataagta ctgcaaaaca
     9721 aagagtaaga ggacgggaga atgcatgatg gagtttcagt tagaccaaga gtggcggtta
     9781 cgtcccatca aaggcgacac aggaaaaaca tacataggca tgcgtgatga ggacaaagtc
     9841 tttattaaac gaaacacgac tccgatgttg gctgctcttt caagggaagg aattacacca
     9901 aaacttgttt ggacaaaacg aacggggaat ggcgatactt tgacagcaca agagtggctg
     9961 gaaggtcgct tgctgactcc taatgagatt ggtcaaagaa acgacgtgat tgatgttttg
    10021 taccatttac atcattcaaa ttctcttaaa accatgctga cccgtatcgg tggtcgtgtc
    10081 atgacgccgg agaagatgct gcaaaaatat gaaaaagaat taccggaaga actccagaaa
    10141 aatcaatttc tgcaaattgt ccatcgtttt cttcgtgagc agatcccgtc aatagataag
    10201 gaaaacttat cagtagtaca cggagatgta aaccatcgta attggttagt atgtaaaaat
    10261 tatttatatc tagtcgattg ggattcaatc atgtttgcag accctgcggt ggatattgca
    10321 acgattttag gaaattatgt gccactgtct agctggaatc agtggctgat cagttatggc
    10381 atacgcccga ctgacgaaat gcttgaaaaa tttcattggt atgcggccat gaatttgctc
    10441 caagaaatca ccagatattg caaacgtgaa gatcatcgac ggatgaacga agaaatttta
    10501 caattaaaac gaattttcag tggctgaggt ttggcccaaa ctctgcggag atttggccag
    10561 acctttttaa ttagaggaaa ggaatggaga taaataatgc gtgtccgaaa tcgtccggga
    10621 gcagcggaaa tgttagcggc tcacccgaat tttgtaatta gtgatccaac attgtggaag
    10681 ggaaaatgga atgaattgtt tgaaaacgat catccaatcc acattgagat tggtatgggg
    10741 aaaggccagt tcatcactgg aatggcaaaa gcccatcctg aaatcaatta tattggagta
    10801 gaaatgcagg tcagtgttgt ttcgattgca ctagacaagt tgatcgaaca accgcttcct
    10861 aacctgaaac ttctacatgt ggatggatca gcgttgacag agtatttcgc tgacagcgaa
    10921 gtagatcaaa tctatctgaa tttttccgat ccatggccaa aaaaacgtca tgaaaaacgg
    10981 cgattgacat ataagacttt tcttgctgta gatgaacaga ttcttcgtcc aaatggagaa
    11041 atccatttca aaacagataa tcaaggattg tttgagtatt ctctggcaag tttttctcaa
    11101 tatggcatga tcctcaaaca ggtatggtta gatttacatc aaagtcaatt tgaaggcaat
    11161 attatgacgg agtatgaaga aaaattttct tcaaaaggtc aacgtattta tcgagtagaa
    11221 gctcaatttc aagataagac caaaaagaaa agtctgtgaa actaactttc tgctttcata
    11281 aaaacattaa tatccataaa taaatctcag aaaatgaaca ttacagatgt aatgtatttt
    11341 ctgggattta tttgatttaa aatgtattta tttcatcggt ttttccacaa ggtggaatca
    11401 attggaagcg agaaaatgaa aataatcctt tgtcggtgat cttcaattcg ggtatgacag
    11461 ataatgttaa gaaagaaagt gtcaaaaaag gatcaaaggt tttagctgct cctagttgat
    11521 gtgctttagt tattaattca ttcaactgtt catagacgat cgaaaaaggt tcctgtgtca
    11581 tgattccgcc tataggaaga gaaacacaag ctatttcctg ctgtttgttg acaacggtca
    11641 tcccgccacc tttttgaatc aaaagattag cagcatacag catatcctcg tcgtttgtcc
    11701 caacgacaac tagattatgc gagtcatggg caatcgttgt cgctatcgct ccttgtttca
    11761 gctgaaatcc tttcactatg cctaatccaa tgtttcccgt ttgatggtga cgctcgatca
    11821 ccgcaatctt taattggtct ttttcgattg aaggaatgaa tcgttcgcct tgtcggtcca
    11881 ctttttctac caaatcttta gtcactagac tattcgggat aatttcgatc acatgggctt
    11941 gtgaagaaga aagaagcagc tctaacgaat gtggatttaa tggttgagcg tttagttttg
    12001 gcagacaaca cgtatatgaa tttgaatgat cagcaaataa atggtcttgg agttgcccag
    12061 attgaacaac taatgttccc ttatagaaaa cttcatgaat tggcagcttg gtaagatcgt
    12121 ctgttaagaa gaaatcagct tgatacccag gggcaatcgc acctagtgtt ttcaaatcaa
    12181 aacattctgc tgcattcaat gttgccatct gaatggcttg taatggatcg attccaagtg
    12241 caatagcttg acggacgata tgatcgattg atccttcttt tactaaatca ttgatcagtt
    12301 tatcatcggt gacaaagaag catcttcttg aattttcagg tgtgatcgcc ggtagtagcg
    12361 atgccagatc ttttgcaacg gtcccttctc gaaccattaa atacatacca agatccaaac
    12421 gttctttagc ttcttttgca gttgtagctt catgatccgt ccgaatacct gctgcaggat
    12481 aaacatttaa ctcttccatg ccgataccag ctgcatgccc gtcaattttt ttcttatgct
    12541 ggtgcatcaa ttggatcttt tctaggatgt ctgtttcatt atttgctact gcttgatagt
    12601 tcatgacttc agctaagcct aaaacttcgg gtttttgtaa aaaaggggct aatttttttg
    12661 catttaaatt tgcaccattg gtctcaaaag gggttacagg tacacaagaa ggtagcataa
    12721 aaaagatatt catgagtgtt tgtcttgcat cttctatcat aaactcaaga cctgtactcc
    12781 cagcaatgtt tcctatttca tggggatctg tcactacagt agtaactcca tgaagcaagg
    12841 atacttttga aaattcgctt ggtgataaaa gtgagctttc gatatgcaca tggccatcga
    12901 tgaatcctgg cactagatat tgcccattag cattatgtac gctttttcct tcgtattcac
    12961 cgattcctac gatcattcct tcgcagattg ctacatcttt ttccatgatt tctttcgtaa
    13021 agacgttgac gatcttagca tgcttgatca ctaggtcagc aggctttttt ttacttgcta
    13081 cttcgatata tttccttaat gttgtttcca atggagtgcc tcccaagata aatttttata
    13141 agtataacat aaattcggct tttgccaata tttttttgta aattcatttt atttgaaaca
    13201 aaaagatcaa aaagacgtat gttttttgtg gaggggagaa taatggaaat aaaacgatta
    13261 tcggatcaaa tgataagtga aagttttgcc aaacatgccc aagacgtata tatattgcca
    13321 gcttcagaag tattttacat ctattttcgc tatggaaaaa aacgaacatt ctggaagaaa
    13381 gtctcttcgg cagatggaca gcaactgatc tcaagattta agtatttggg acagatggat
    13441 atcggagaaa aaagaaaagc tcagttaggt gcatttactt attctttaga aaaacaggaa
    13501 gttcgtctgc ggttgtcttc tgtaggagat taccttcaac gagagagttt agtgattcgg
    13561 ctattatacg gtacttttga aacaccccat cgttgcttta gttctcgaga ttttgcaact
    13621 attttacaga aaaccgtcag acgtggatta taccttttca gcgggacagt cggatcaggc
    13681 aagacttctc taatgtatcg gttggctttg gaagaaaaat tgcaagtgat tactatcgaa
    13741 gatcctgtag agatagaaga accgtttttt ctgcagctac agatcaatcc gaaaatagca
    13801 cagacttatg atacactttt gaaactcgca ttacggcatc gtccagactt gctaatcatt
    13861 ggcgagataa gagatgaatc aactgcacat gctgcggtaa gagcagcact aacagggcac
    13921 cgggtttttg caaccgtaca tgcacgcaat cttgaaggta cattggtcag aatccatgaa
    13981 ctgacaaaga aaaaagaaga gctatctgaa tgtttagctg gtgtcattta tcaagaactt
    14041 ttggaagatg ccaatcacca tccttctgtt ttatggagtt atcaatttat ggaggaagaa
    14101 tcagtaaaaa aatcatggga ggaaagttat gaagaagctc aggaaaatat ttggcagtca
    14161 gaactattct aaaggattaa caagaagcca gcaaaaccag tttgcacaaa ccatgggaga
    14221 actgctttta tctggtttta gtctgcaaga atccctttat ttattactca cgatccgttc
    14281 gtttccacct gttattttgg caaatatcca acatcagcta aaggaagggc aagcgtttag
    14341 tgaggtactt tcgcaaatag gatatggagc agagcaactg gtacagatcg agttagctga
    14401 acgtcatggg aatctgccga caactttgtt ggaaattgcc aaacagtttc ggcttgttga
    14461 aggatttcgc aatgatttga aaaagctgct tgcatatccg atgctattgc tcaccttttt
    14521 gctaggtatc ttgtttagta tgcggttgtt cgttttgcct cagcttattg gttcaggaat
    14581 ggtcatgcaa gagcattggg gtattcagct gatacaagtc tttcattggt atgttctact
    14641 attcatccta gcggttactc tattggcttt actagtaagg tatcgtctga tgaagttgtc
    14701 tcctgttgat cagagtgaat ggataagcag acaattattg attggaccga tatattcctt
    14761 gtatcaatcg gcttatttgt cactagagat tggaaagctt tttcatgaag gtttcgaatt
    14821 aaggcaaatc atagtctgct taaaggaaac gaaacaagaa agtctgattc agcatcttgc
    14881 ctctaagttg attcaaggat tggaggcagg tatgtcattg gctgagcagt tccagaatta
    14941 tcgttttcta tcagaagaat tcagtaaaat tgttttacag ggagaggcaa agggaagttt
    15001 aggtaaagaa ctgatttttt acagcgaatg gacaagaaaa cagcttttca aacgtcttca
    15061 tcactggatg cattttattc agccaatcat ttttttgggc gtagcggctt tgattttatt
    15121 agtttatgcc gctgttttat tacctgttta cggaagcata gaggaggttt tgccatgaaa
    15181 aagagaaaaa tatcttatta tagcggtttt acattgatcg aaatgctaat cgtcttactg
    15241 atcatcagtg tattagtgtt gttgttcgtc cctaatttgt cgcgttatag gaatcatgta
    15301 gaccaagaaa gtcgtgaagc gatcatccag ttggtagaca cacaaaaaga gctctatgcg
    15361 cttcaaaata atggaagagt tcctactgtg gaggaattgt tgaatgaagg ctatatcaaa
    15421 cgagaacatg cagaaatcta tcagcggcct tgaaggtaag gttttagcat caggatatac
    15481 gttgtttgaa tcactcttac tgattttatt acttacaacg atcctttctt ttccagttct
    15541 cgcattttct gcatggaaga atgaattggc agtctatcaa tttttcggcc aatttgaaaa
    15601 acgaatctat tctacgcaaa aaatcgctat tgtgaatcag atccaaacag gcttttattg
    15661 gaatagtgaa gagaatcaaa tcatttttca tgttccgaat ccagataaaa taaattggga
    15721 agtattagat attcctgatg agattacctt gaaacgccat gcttctatta cttttgctgc
    15781 tagaaccggg aatgaaagtt cactaaaagc ttatcaattt tattgggaag aaaaagagcg
    15841 gacgattacg tatcaattcc agatagggag tggtcgctat acaaaaagaa ttgaataaaa
    15901 caggatttgt tttgttggag tcccttacag cgcttatgat cgcttctgtc tgtgtctact
    15961 tagtgagctt tggccagctg tttttgcttc aggcagaaaa gaaaagtggg gaggaactgc
    16021 tatttattcg aatgatttat gaagatatca agacgcagcg gatgtatcac aaaagaccag
    16081 agcgaaggga agaaatgatc ccatacgata tccaaattgt cgataataat ggagtaatcg
    16141 aaaaagtgtt tgtcaatatg gagaaagaag cgatcagtat tgaaaaaaca agtaattcca
    16201 gcatttacgc tgattgaatg tctaattgga ctgatgatat tgagcagtct attgctcaca
    16261 ttcaatcttt tgattcagca aaccatccaa gtaagtgaat ttttgcagcg taaagatcaa
    16321 aaagagtggc taatattttt agcacaatta gaagaagaac tcaagagcag tcacaatgtc
    16381 tctgtgaaaa atcagcaact gcactacacg atcgaggata aacagtatat catcgaaaga
    16441 tatcaggcaa tgatacgtaa aagaagaact tccggcggac atcaacctat gttaacaagt
    16501 atcactgagc ttcaattatt ggaaaaagaa cacacaattt ctttttatgt acactttgaa
    16561 aatggagaag atggatatgc catatggaca aaaaacgatc agtagaaaat gttataaggg
    16621 ctctatttta ttttctgtct tgcttttatt tcttttatgt acgtttttat tgatggcagc
    16681 attagaaagc taccgtttgt caatggatct taatatacga atcaaaaatt attatatagc
    16741 aaaaataatg aagcagatgg ctttatcgga acagtctacg cttgccgaaa gttcggaagg
    16801 agttttttac tacacaactg gatctgtaac atatcagtgg gtacaacagt ccctattctt
    16861 agaggtggaa gtctcaccgt ttatctttcg gttcacagaa gaagtaaaaa atgatacaga
    16921 tactagtaca gaatgatgga atgttcgtga aaacgccctc ttttctgtta taacactgtt
    16981 tttaataaaa aaatgtttgc cgtttcacaa ataaaaaaca gtgaaaacga ttgaaatttt
    17041 gtcaattgtt cgatagaatt aaacaggtga aaaaattgtt ataatatatc tagaaatgag
    17101 gtgcaccatg ttaccagaaa aaatggaaca agggtttaat caaaacctgg aagctattca
    17161 gcttttacag aatgcattgg gaacttcctt tttagaagcc tatgtcgaaa atgcagaaaa
    17221 tttaatagat gattatcaag tacgtgtggt ggatggagtg ccgacaaaag agacaacgca
    17281 acggatcaca gctctttatg aagagttgaa gaaactttct ttcgagccag aagaatggcg
    17341 cagactgtct caattacttt tattgaaggg cagccagaca gaacatttgc aggcgaacca
    17401 tcaactcact cctgatagta tcggtttttt atttgttttt cttattgaac aactatacac
    17461 aaataaaaaa gcaccggtta agattctgga tattgcggca ggaatgggca acttgttatt
    17521 aacagttctt ttaaacctat cgaatgcggg gtaccaaaca gaaggaattg gtgtggatat
    17581 tgatgataca ttgttggctg tcgcagcttc tacaagtgat ttgacacaag caaatgttca
    17641 atactttcat caagacgggt tgcaggaatt attaatagat cctgtcgatt ttgcaatcag
    17701 cgatttgcca atcggttatt atccgaatga cgaaaaagca aaagagtttt taacaagtac
    17761 cgaagaaggc catagttatg cacatcattt attgctagag caatcaatga aatatgtgaa
    17821 gccggatggt tttggtttgt ttttgatgcc gagtggattt ttagaaacgg atcaaagtga
    17881 agaaatcaaa aaatggttta aagaagaagg ttatcttcaa ggaatgattc aattgccaga
    17941 tgaattgttc cgtaataaac aatcgcaaaa aagtattttg atcttacaga aaaaaggacc
    18001 acaagcacaa caagtaaaag aagtcttgct tgtgaaactt gcttcattaa aagaaccaga
    18061 gaaggtgacg gaatttttca acgagtttaa aaactggaaa tcatctgctc tgtaatcatt
    18121 caacgagaag gagagtcata tgtctaaaac aattgcaatc aatgctggga gttcaagttt
    18181 gaaatggcaa ttatatcaaa tgccagaaga aaccgtagtt gcgaaaggga tcgtcgaacg
    18241 tatcggttta caagattcta tttttaccat caagtacggc gataatcaaa aattcgaaca
    18301 aatcatggat atcgaaaacc acgaagtagc agtaaaaatg ctgttagatc agttgattga
    18361 actgcaaatt ttaagctctt atgatgaaat caccggagta gggcaccgtg ttgttcacgg
    18421 aggagaaact tatggggatt cagtagtgat cgatgagaaa gtaatggacc gcattgctga
    18481 attagcagaa tttgctcctt tgcataatcc tgcaaactta atggggatca aagcgtttaa
    18541 aaagatttta cctgacattt tgagtgtggc tgtattcgat acatctttcc actcaacaat
    18601 gccaaagcaa aattatttat atagcttacc ggtgtcttat tatgaagatt tcaaagtaag
    18661 aaaatatggc ttccatggca caagtcatcg ttatgtgtcg gaacgtgcgg caaaattatt
    18721 gaacaaaccg atcgaagaac taaaaatcat cacttgccac ttaggaaatg gtgtatcgat
    18781 cacagcagtc gatggaggta aatccatgga tacttctatg ggattcacac cattagctgg
    18841 ggtgacaatg ggtacacgtt caggtgatat cgatccagct gttctcccat atttaatgga
    18901 aaaattagag attgatattg atgaaatgat caatgttttg aacaagaaat ctggtctgct
    18961 aggcctaaca ggattgtcta gtgatatgcg tgatcttgaa aagaactatg aaaaagaaca
    19021 tattcgttta gcttatgaca ttttcgtgga ccgcatccga aaatacatcg gcggttatgt
    19081 gacaacgatg aatggtgttg atgcaattgt ctttactgct ggtatcggcg agaatgacgg
    19141 acatgtacgt agtgaaatca tcaaaggtat gtcttggttt ggttgtgaaa tcgatccagc
    19201 gttgaatgaa ttgcgtggtg aagaagtaga tatctctaca aaagaatcaa aagtaagagt
    19261 attagtgatc ccaacagatg aggaactaat gattgcccgt gatgtggagc gtctgcgcgg
    19321 ctaagccaaa gcattcgtta ggataaaaga tgaaacgcat gcaagaggga attcttgcat
    19381 gtgttttttt tgcggagaag accgcaaaat ggtaatttaa cttcaatttc ccttcttttt
    19441 tacaggatct tcttagggaa ttttgcttgg aaaagtggtc aaaagggaaa agactggcat
    19501 tttatttatt gattttcagg aaaatcccaa tattttattt tcattcatct ttataaggga
    19561 cattacatat gtaaaaagaa aaaacatttt tagatcagcc tcgtgggctg tcgactttat
    19621 tcttcgcttt atgtttagct agaaaaccga tccgaaacat tcattgatcg aataataaaa
    19681 acttgatttt ccacctgtac tttgcctgaa tcgtgaaagc acaggtatga aaatcaagtt
    19741 ttttgttgcg aaaaaacgtc ctagtcaatc agttggccga gcggctggcg gctgatctgc
    19801 tcctgatcaa taacgagtgc ttcttggaag tgttgctgca aaaagtagcg gtcatggctg
    19861 actgctagga gcgttccttg atagtcttgc agcatttctt caatttcttc tcgagcatag
    19921 ctgtcgaggt gattggttgg ttcatccaaa atcaggacgt tgattttttc taaaaagagt
    19981 ttcagcaacg ccaaacggat tttttcacca ccggataaat ctttgatccg tttacccaca
    20041 tcgctttgat aaaagccact tttggctaat tgccgtcgag cgacttcttc gtttccagtc
    20101 aatgtgatgg cgtaagccag caaacggcta tcgggatcgg caaactgcag ctgctgcggc
    20161 aaataaccga tttgcaagct actacctaat ttgatggtgc caccgtaagg cttttcctca
    20221 ccgagtaata atttgagtaa ggttgatttt cctgcaccat tgtcgccgat gatggctaaa
    20281 tgatctccac gatagactgc aaaactgctg tctttgaata gtacttgatc agcgtactgc
    20341 tggtgcaagt tttgaatgat gaaaacttct ttgccagatt ttccaccatt agacaagctt
    20401 tgcagccgat ttttcggggg aggtggcggt ttgacgagtg tcagtttcgc caggcggcgt
    20461 tccagttctt tggctttttt gaagaaggac tcattgtctc cttcatgtgc ccactgacga
    20521 aaacgacgaa tcatgacttt caaccgctgg acttcttttt gctgcagttc gtaatttttt
    20581 gtcagctcag ccaagcgagc ttctttcaag actcgaaaac ggctgtagtt tccgggatac
    20641 tcaatcaatg caccttcttc cagttcccat gtttttgtgg tgacttgatc gagaaattgc
    20701 cgatcatgag aaacgaccag aaccgatttt tttgtattct gtaaatagtt ttccaaccag
    20761 cgaatactgt taagatctaa atgattggtc ggttcatcga gtaaaagtaa atcggcatcg
    20821 gctgctaaaa ttttggctaa agcgacttta actcgttcgc ctccgctaag ttctgttaac
    20881 cgtgcttcct gacagtgtgg aatccctaag ccttgcaaga tcgctgcaat ccggtcttct
    20941 aatacgtaac cgcctagttc ttcaaagcga tgctgcagct caccgtaacg ggccagacac
    21001 ttctctaagt ttgtatctgg ttctgtcatc aatgcttcgt agcgactcat ttgctgtttg
    21061 aggtcctgta tctcagcggc agaatggagc aagtagtggt tgacggtcgc ttcggaaact
    21121 gtaagttctt gttcgaccgt cccgatcttt aagccttttt tgcgactgat aacaccgctg
    21181 tcgacacctt ctctgccggt caagatttgt aaaaaggttg atttgccagt tccgttgata
    21241 tcgactaagg caattttttc accagcttca atttgacagg ataaatcctc aaataatgga
    21301 ttcccgccga ttgttttttt aatagaaatt gcttgtatga tcatgatctc tctccttgag
    21361 accactcaaa aaggccatgg ttatccctgg cctgtgttat ttaaactcag attatcatga
    21421 gaaaaatacc ttgagatggc cattgatttt acgtgaattc aactgttttg gacaaacttg
    21481 tcccgtcaac agcaactgta aaaccaattg catggctaaa tgcgatatgg tatcgttggt
    21541 aattttcatt acttgttatt ttagtcatgc gcatcatagt attttccctc cttcgtgtag
    21601 ttgtcttttc cagtgtacct gaaagtgtca gaaagttcaa ctagcgctga catttactta
    21661 atatttatcg atgtgtaatt ctgtcagaga gcttttttta tctgactgag ggtaatgtct
    21721 cgtggaccac atcattgtat tctgagctaa accagcgttc aggcgaaatc atatggtgtt
    21781 tgataaaaaa ctttgtctac atgacttcct tttgccattt gcttaatacc gttgcattaa
    21841 acagctcttc tctttggaaa tctcaccaac atcgcgttca ggagtatcgt tgtggttgca
    21901 atggcaagcg gtagcctttc atcaattctt gaatgattta ccgaatgtag cacttgcctt
    21961 ctcgcggtac ccgaacccga aataaaattt tagtttggcg ataaaatcgg tatccgccat
    22021 cgatacttca tattttttcg tatcagattc atcggaaact tccacattga cgaaccaata
    22081 agctttggca tgcttaggtc gttttaaacc acaccagggc gactaatcgc cctggtgttc
    22141 acttttttta ttttacttga actcattgac tgttttccct ttattttaaa cattataaag
    22201 accgatgagt gcttgccgcg tcttttgttt ttatcacggt tttttgtgca aaaaaacctg
    22261 ccgatagttg gcaggtcgtc tacgtgttaa gctttggtgt ggtctttagc tggcaattgg
    22321 ttttctaaat cagttcggac aacaagcaca tcgcaagctg cgtttctaat aacatattca
    22381 gaaacagaac caataaataa acgttctaca gcattcaatc ctgtggctcc taacataatc
    22441 aaatctactt gattatcttc tgggatttct ctggcaataa tttgttttgg tgagccatat
    22501 tctacgacag aggttacatt attcaagcca gctttcttcg cattgctttc ataatctgcc
    22561 aatgtttgct ttgccatttc agtagcttgt tctgcaagca ttccatcaaa ggatgaaacg
    22621 gtttggaaag aacgtgtgtc aatgacatgt gctaatagaa gttctccgtt atttcggata
    22681 gctacattga ccgccttttt aaaagctaat tcagcttcat cagaaccatc tacggcaacc
    22741 atgatttttt tgtattgttg taacataagc attcactcct caagtcaatc gtattgtact
    22801 cttttattgt agaacacttt agggaaaaag agaattattt actgctttgg ttcagcaatt
    22861 ttttttgcaa atcggatact gaaggtggca gaaacaataa ttacgatcag taaaaagacc
    22921 aaggcaataa agcgttgatt gaaaaaagct gttaaaatac ccattacgcc taaaacagca
    22981 tatgcagcac cgtaaaattg taggaatcct tgatttttat catttttgtt gatcaataca
    23041 aaaaagatat cctgtttatt aaataaataa tagctaacga ataataatac taaagtggcg
    23101 ataatcatta aaatcctaat catgaacaaa tcctccaaaa taagcaacaa atagaaaaag
    23161 caatttgcca agagcaaatt gctttattct ttaaaagatg gtaatctccg ttaatgccgt
    23221 tgtttgtcct gtagtattga tcccgcgaat tcaagcaggt gggtcccatg gtcgtagctt
    23281 cgaactacca aatgataagg catgttacca gcagtgacac aagacgggtc ccaagatatc
    23341 aataaaaatg ttcggtcaac acacaaagag gacaattcga acatcacaga tttcccacct
    23401 ctatagtagc atatgaattt aaaaagagca atgagagagc tcgcagaaac agaaccgttt
    23461 tcttgtctag ttttttggag gatgcttttt ccactctttt atacggtaat actgttgacc
    23521 cagagcctgt tcatatttcc ctgtatcttt tggctgataa tactgagcat tcttcagttt
    23581 atctggtaaa tattgctgat tgacccaagc ctgatcaaaa tgatgaggat actgataacc
    23641 cacgccacgg ttgagtgatt tcgcaccttt gtaatggcta tctctcaaat gatcaggaac
    23701 atctccagct ttgccctctc gaatatcagc aagtgctgca tctaatgcca tgtaggcaga
    23761 attggatttt ggcgacaagc aaagatcgac gacgacgtct gctagtggga tacgtgcctc
    23821 aggcaatccc aatttttcag cagctaatac agcgttcact gtacgagccg cagcagcagg
    23881 attacctaaa cctatatctt cataaccgat aaccatcaga cgccggcaaa tacttgccag
    23941 atcacctgcc tcaactaaac gggcgagata atgaagtgca gcatccacat cacttccacg
    24001 aatcgatttt tgaaaagccg aaataacgtc ataatgagca tctccatttt tgtcatgggt
    24061 aagagctttt ctttggacac attcttcaat gatcgacagt gtaagatgaa tccttccttc
    24121 tttatctgaa ggagtcgata gtgtagccag ttccagccca ttcaatgcac tccgcaaatc
    24181 gccgttcgtt gcacgcgaaa ggtgaagaag agcatcttcg tctaactgga ttgcttgttg
    24241 tccaaggccg cgttctttat cccttaaagc atgttcgact gccaactgga tgtcttgttc
    24301 tgttaatggt ttgacttcaa agatctgtgt acggctgcgg atcgctggat tgatggtaat
    24361 ataaggattc tcagtagtcg ctccgatcaa gatgattcgg ccactttcta aatgaggaag
    24421 cagaaaatcc tgttttgttt tatctaatcg gtgtacttca tccaataaga ggatcactgt
    24481 accactcatt tttgcttctt ccgctacaac ttgtagatcc ttttttgaat ctgtagcggc
    24541 gtttagcatg cgaaatgcat aattagtaga gccggcgatc gcacttgcga tactcgtttt
    24601 tccagtacca ggaggtccat atagaatcat ggaagagagc atgcgagcat cgaccatgcg
    24661 gcgtatgatt tttccttccc cgactagatg ctgctgacca acgacttcat cgatcgttcg
    24721 aggtctcatg cgataagcca atggctgttg cataggattt tcctccttct tgtttttacg
    24781 tcccttaatt atatcatatt tagcaagatg cctagaaaga acagccttct gttgttttgt
    24841 tttcatagct tatccaacta atattgtgta tgataaagta ggtgagaaaa atgacggata
    24901 aacaaaagat agatgaattt ttttggaata aaacgacgcc agaagtggca cgggatttat
    24961 tggggatgta tttggagcat gacacaccag aaggaagatt agctggatat attgtagacg
    25021 cggaagcata cctaggtcca gaagacgagg ccgcccatag ctatggttta agaagaacac
    25081 cgcgtgtgcg ggcaatgtat gaaaaaccag gtacgatcta cctttacacg atgcatactc
    25141 atcggatttt gaacatcatt actcagccag aagggattcc tcaaggagta atgatacgtg
    25201 caatcgaacc cgccgcaatg attgatcaaa tgagtaaaaa tcgtggcgga aaaactggtc
    25261 cagatatcag caacggtccg ggaaaattag tggaagcatt agcaattcct caagaacttt
    25321 atggtcagtc tatagcagat agttcgttac gcttagtttt tgagaaaaaa aagacaccaa
    25381 aaaagatcaa tgctttgcct agaatcggta tcccaaacaa aggtgtatgg acagaaaagc
    25441 cgttgcgttt tgttgtgtcg ggaaatcctt atatctcttt acaaagaaaa aaccaaatcg
    25501 agaaagattg gggttggagg aaagaaaatg aaaaagaagg atctattaac atatttagat
    25561 gaaataattg aaaaacaagt gacagactat gatgtagctt tagattggga tacaaaaaat
    25621 catacgatcg agatcgttgt tcgcttgttt gcagaaaata aagcacaatt tgagattgat
    25681 gatgcagaag ggatcgtttc gcaagaacca attatcgaat ttgaagacgg cgtacttctt
    25741 tttaatcctc agaaatctgt ttttgatgaa cgcaattatc tagcagtaat cccttatgaa
    25801 ggaaaaaaag ggttggcaaa atcagtagct gattctttag tggaatatct aaatgaagta
    25861 ttagctcaag ggcaaagcga tttgcttgat tttttggatg aagacgatga cgaagctgta
    25921 ttcgagcttc actgggataa tcaacaacta gcagaattag tagaagtaaa acagcaaaat
    25981 gaagcagata cttatttacc gtacccaagt tattaaggag tgacagatat gaaatggaat
    26041 gaagtaaaag tagaaactga aagtgaagca gtcgaagcag ttgctaatat tttgatggag
    26101 gcaggggcga gtggtgtcgc gattgaagat tctttagatg ttgaacattt tcagtcggat
    26161 caatatggtg agattttaga taaaaatgat tttacatcgc tgaaagaagg cgcactagtc
    26221 atggcttatt ttccggaaac gactttctta ccggaaatct tgccttttat caaagatcgt
    26281 atcacacaat tacctgagtt tggtctagct attggaaaaa ataaagtaac agttagtgaa
    26341 gtagaggaaa gcaactgggc aacagcctgg aagaaatact atcatccggt gcgtattacg
    26401 cgaatgctga cggttgcgcc aagctgggaa aactaccaga cagaagatcc tctagaaaaa
    26461 attatcacac tagatcctgg tatggcattt ggcacaggca ctcatcccac gacttccttg
    26521 acattgacag cactagaaac agtcttgcgt ggtggtgaaa cattgctaga tgtgggaact
    26581 gggtcgggcg tgctaagtat tgcagcaaaa tatcttggtg caaaagatgt ctatgccttt
    26641 gatttagacg aagtagccgt tcgttcggcg aaagaaaata tggatatgaa tgaaatagcc
    26701 aaagatgtcc atgtatcagc taacgatctt ttgacaggaa tcgagattga aagtgatgta
    26761 atcgttgcga acattttagc agatatcatc ttattgatga tcccagatgc ttggcgttta
    26821 ctgaagcaaa caggcacgct gatcgtttca ggaatcattg aagagaagaa gcaaatggtt
    26881 ctggatgcca tgttagaaca agggtttatc gtggatcaaa ttttccagca aaaagactgg
    26941 tatgcaatta ttttgaaaaa accagaggtg gaatagatgc agcggtattt tttaaaagaa
    27001 tcatatgaat caaaagacta ttttgtttta gaaggggaaa actatcacca cattgtacgg
    27061 gttatgagga tggaacccga ggatgaagtt tttttggcat tcaatgatca aatagccatt
    27121 gttgcagaaa tcacggagat tactgaagag gctgtttatc tgaaagaaat taaaaaagaa
    27181 gaaaatcaaa aggaactgcc aagttcgatt accatagcga gtggctatcc taaaggggaa
    27241 aagctggatt ggatcgttca gaaatccact gaattaggag cgcaagcttt tcttggtttc
    27301 ccggcatcag cctctgtcgt taaatgggat gcaaaaaaac gtatgaaacg acaacaacgg
    27361 cttgaaaaga ttgctcaaga ggcagcggaa caatcccatc ggcagatcgt gccatcagta
    27421 gagttattgg aaacgaaaca agatttattt gagcggctag atgattatga cgtggtcttg
    27481 gttgcttatg aggaatcagc aaagcaaggc gaaaaatcaa atgcagccaa aattcttagt
    27541 gaaacaaaac cagatagtcg aatcttagct gtctttggac cagaaggtgg attatcccgc
    27601 gatgaagtcg attattttgt agaacacaaa gggatacttt gtggattagg accaagaatc
    27661 cttcgtacag aaacagcacc tctttattta ttaagtgctg ctagctatca atgggaatta
    27721 atgaatcctt ctttcagcta acgttcttca ttaataaaag agtatcgaca gaaagaattg
    27781 aaaaaatagt caacaatttg tataatagaa acaattattt atacaatgga tacgaaaaat
    27841 cgtttgaaaa agcttttaaa aagcgaacat ttccgtaaaa aaacgtataa ctgcacggat
    27901 tttgggacaa ttatcaaaca gatatttgtc ccgattcccg ttttaccgaa aaaagaaagg
    27961 gggatcgatt cctgtgacaa aagaagaaat tttaacggga cccggtgtca taaagctcgt
    28021 tagtcaatac atgggtcccg aacatgtagc atttgttgag aaagcatgcg aatacgcgac
    28081 tgctgcacat gacggacagt ttagaaaatc aggcgaaccc tacattattc atcctatcca
    28141 agtcgcaggt atattagcag atttaaaaat ggatccccat acagtggcta caggcttctt
    28201 acatgatgtt gttgaagata cagaaatcac tttagaagat ctgagagaag aatttggcga
    28261 tgacgttgct atgttagtgg acggcgtaac caaattaggg aaaataaaat ataaatccca
    28321 cgaagagcag ctagcagaaa accaccgaaa gatgctgcta gcaatggctc aagatttacg
    28381 agttatcatg gtcaaattag ctgatagact acataacatg cgtacgttga agcacctgcg
    28441 agaagataag caaagaagga tcgctcagga aactttagaa atctatgcgc ctcttgcaca
    28501 tcgtctaggg atcagccgga tcaaatggga attagaagat acagcacttc gttatctaaa
    28561 tccaaaacag tattaccgta tcgtccactt gatgcagacg aagagagaag aacgtgaaaa
    28621 atacgtaagt ggtactgttg aagatattcg aatagcgaca gaagagctgg ggatttttgc
    28681 agaaatctat ggacggccaa aacacattta ttcaatctat cgtaaaatga aagatcagaa
    28741 aaagcagttc aacgaaattt atgacttgct agctattcga gtgatcgtag attcaatcaa
    28801 ggattgttat gctgtattag gagcaatcca cacaaaatgg aaaccaatgc ccggcagatt
    28861 taaggattat attgccatgc caaaagctaa tatgtaccaa tctttgcata ctaccgtgat
    28921 cggtccggca ggaaatccgg tagaaattca aatccgaaca caggaaatgc atgaaatcgc
    28981 tgaattcggg gttgctgcac actgggccta taaagaagga aaaaatgaaa aagtagaacc
    29041 agatggtatg acgaaacaat taagctggtt ccatgagata ctcgaacttc aagacgaaag
    29101 ctatgatgct tctgaattta tggaaggcgt aaaaggagat atctttagtg ataaagtcta
    29161 cgtcttcaca ccaaaaggag acgttactga gttaccaaaa ggatccggac cattggactt
    29221 tgcatacagt atccatacag atatcggtaa caaaaccact ggtgcaaaag taaatggcaa
    29281 aatggtgcag cttgattaca aattgaaaaa cggagatatc attgagatca tgacttctcc
    29341 aaattcattt ggcccaagtc gcgactggtt gaaattagtt gctactagca aagcaagaaa
    29401 taagatcaaa cgtttcttca aagcccaaga tcgagaagaa aatgtgatca aaggccacga
    29461 atccgtggtc aaatgtatta cagatctagg atttacgcct aaagatattt tgacgaagaa
    29521 caaactgcaa gaagcactcg atcgttttaa ttatcaaaca gaagatgatc tctatgcagc
    29581 tgtagggtat ggagaagtta gccccttgac gatggccaat cgtctgactg aaaaagaacg
    29641 taaagaacaa aaaatcgagc agcaaaagca agaagcagaa gaaatcatga atcagccgaa
    29701 aaaagaacct gacaaaatga aagtacgtca tgaaggtggc gttgtcattc aaggggtaga
    29761 aaacttacta attcgtatca gtcgctgctg taatccgatt cctggtgatg atatcgttgg
    29821 ttatatcact aaaggcagag ggatatccat tcatcgtcga gattgtccga atgttcagcc
    29881 tgacaaacca aatgtagcag aacgtttgat tgaagtcgaa tgggaagata catcgaatac
    29941 acgaaaagag tatgatgcag atttggaaat ttacggctat aatcgttcag gcttattgaa
    30001 tgatgtactt caaacagtca atgcgctaac gaaaaatctc aacagcgttg aagcacggac
    30061 gaataaagat aaaatggcga cgatccattt gacggttggt atccagaatt tatcccacct
    30121 aaagagtatc gtggataaaa tcaaagcagt acctgatgtc tacagtgtac gccggacgaa
    30181 tggataggag gacagatatg agagcagttg ttcaacgggt aagtaaagct gctgtgtcag
    30241 tcgatcagca agaaattgga aaaattggct acggcctact tgttttatta ggcattcatg
    30301 aaaccgatac gcaaaaagat gtggactatc tggtaaaaaa aattgcacaa atgcgtattt
    30361 ttgaagatga acaaggaaaa atgaatatta gtgtagaaga tgtgggagga caaattttaa
    30421 gtatctctca attcacactg tttgcggata cgaaaaaagg aaatcgacca agctttgtgg
    30481 aagccgcacg accagaaaca gctattcctt tatacgaatc attcaatgaa ggaatcagaa
    30541 gtcgcggcat cacagtcgct accggacaat tcggagcaga tatgtcggtt tctctcgtca
    30601 atgatggacc tgtaacgatc attattgatt ctaaaaacaa ataaagtaat ctggttttca
    30661 ctagtaaaaa ctatgaaaca cgtcgcttaa gttagactga taaactctaa cttaagtggc
    30721 ttttttgtct gcaaaaagcg cttcttttgt cagcagtcgg aaaaaaagtt aagcttaaat
    30781 tatacaagtt attttttata ggaggttgct gaagatgaaa gaagtaccag tagtagatat
    30841 caaaatcaca gatttaaaaa aattgtatca aaaaggatac aacatgttgg aggagttacg
    30901 ccatgaagcg gacgctcccg tcgtcaaagc aaagattttc aataaagaag ctatcacgat
    30961 ctacggatca tcagcagcaa aagtatttta tgatccaagg aatttcaaac gaaaaggtgc
    31021 tatgccaaaa ttagtattga agacactttt tggtcaaggt ggagtacaga ctttggatgg
    31081 agctgctcat caccatcgga aaaatatttt tatggatcta atgacaccgg aaagaatgga
    31141 agattatcat cgtatcttag ataagaatct gacacaagca cttgaagcac agcatggtca
    31201 atttgaacta tttgatttaa gtaagatggt tttctttaca tctatttgcg agtgggcagg
    31261 tatcaattta tcagcgattt ctaaagatga ggtcgaaaaa ttagcagaat atcaaatctc
    31321 tatgatcagc ggaacgttta cttctccgat tgatcacata aaaggtgtcg agaatcgcaa
    31381 aaaatcagaa aaatgggcgc aaggcttgat tgaagaagct cgacaaaatc ctgtggctgg
    31441 aaaagaaaat gtggcattat atgcttttgc caatgccaca gatttagatg gtcagctttt
    31501 acctctggaa gtggcagctg ttgaattatt gaacatcatc cgtccaacgg ttgctctaac
    31561 tgtgtgggcg gcattgatgg gacatgcact tttcagtaga ccagatttgt atcagcaatt
    31621 aaaaaatgac ttttcgacgt tacaagatcc gtttattcaa gaaatgagac gctattatcc
    31681 tttcttccca atgctacctg cgatttcgct gaaagaagta gaagtagatg gttatcgtat
    31741 accagaaggc agttgggtta ttttagattt atatggaaca gaccatgatg aacgaacagt
    31801 tgaagcgcca gattccttta tgatcaaacg gtatgtaggg aaagcaaaag atatttccta
    31861 taaagaagaa tatgagatga ttgcacaagg cgggggaaat tttcgacaaa tgcatcgctg
    31921 tgcaggagaa tggatcactc ttcatagtct acgtgtgttt tccgatcagc tagtaaataa
    31981 gtttgagttc tctgtacctg aacaagattg gacgattcca ttcaatcagt ttccgactta
    32041 tccgaatagc cgagcgctac tttacaagaa ttagattagc ataaacttat cgaaaaaaag
    32101 gaagatcaaa cagtgctgat cttccttttt tcatattact ataaattgat aaggtcaatt
    32161 gatcaaaatc cacgaaatgc ttcatcaatg gtttgataat cttcattgga taaatgcaaa
    32221 tctaaagctt ggacattgtc agatacttgc tctggacgct tagctccagg tataactaca
    32281 gacactcgtg gattcttcat gtaccaagct aaaacaagtt gcgcaggagt ggcttggtat
    32341 cttttagcaa tggggcgaag tacatcgaca gcggttaaaa tttctctgaa acgttctcct
    32401 tggaaatctg gatttctttt tcgaggatct ccttcaccaa attgtttttc ctcaccaagt
    32461 tcatattttc ctgtcagcaa accggaagca agaggaaaat agggaacaaa actgattttg
    32521 tttttttcaa gataagggaa taattctttt tcagcctgcc gatggatcaa actgtattta
    32581 tcttctacta catcgacata tccatctgca ttcgcttctt tcaattgttc taacgtgaag
    32641 tttgacacac cgactgctcg gatttttcct gcttctttta attcgtgcaa tgtagcaact
    32701 gattcatttt ttggtgtgct ttcatccgga aagtggatat aaaaaatgtc gatgtaatca
    32761 gtttgcagac gttttagtgc atcttccacc gattgtttga gaaactcagg agaattatca
    32821 aatgttcgcc ctttatttgg tacatgagca gcttttgtag cgatgacgac acgtgagcga
    32881 tcatattctt tttccttcag tacctcgcca atcagttctt cggaacggcc gtttccgtaa
    32941 gcataagcgg tatcgatcaa attgatcccg ctattcaaag ctgtacgaac tacttgtttt
    33001 cctgtctcat cttccaaacc gtcaaaaaga ttatgtcctc caacggcatt tgctcctaat
    33061 ccaagctgtt ctgcgtagac ttgtgaatgt ccgattctta tttctgccat agctgattcc
    33121 tcctttttgt ctagtctaaa acaaaatacc aaaaagctga actaaaacgt ttttgtgagg
    33181 ggaaatagtc agatatctct tgactttgag actggttttt agtaggttat aagtaatatc
    33241 gaatgtcaat gaaagagcga gtaagttttt gaaactttac aagagaggat catcgaggct
    33301 gaaaatgatc taaagtttca ttagcctaaa gacactctgg agatggacga gtgaaaagcc
    33361 gtcccgcgta cgagcgttat tcgtaagagg aaagagaaat ctttcaaaat taggtggtac
    33421 cgtgtatgaa tttcattcac ccttgttcat gatgaacaag ggtgattttt ttatttacat
    33481 agctaagaaa gggagagaaa actatgagtt tccaaagacc aaaaggaaca aatgatattt
    33541 tgccaggcga atcagaaaaa tggcagtttg tcgaagaaac agcacgttta ttattcagag
    33601 attatcagta taatgaaatg cgtacaccga tttttgaaca ctatgaagtg atatcccgaa
    33661 gtgtagggga tacaacggat atcgtttcaa aagagatgta tgatttttat gacaaagggg
    33721 aacgccatgt gacgcttcga ccagaaggta cagcaccaat cgttcgttct tttgttgagc
    33781 ataaattgtt cggcccagag tatgcgaaac cgttcaaaac tttttatatg ggaccaatgt
    33841 ttcgctacga acgccctcaa aaaggacggt tacgtcaatt ccatcaaatc ggagtagaag
    33901 catttggatc agaaaaccct gcaactgatg tggagagcat ggtgatggca cttgatttct
    33961 tcaaacagtt ggggatcacg caaatccggc tagtcatcaa ttcattaggt gataaagaaa
    34021 cgcgtcaagc ctatcgtcaa gcattgatcg actatctttt accttttgaa tcagagctga
    34081 gtgaagactc taaacgccgc cttcatgaaa atccattacg cgtattggac agtaaagaca
    34141 aacgagatca aaaatttgtg gcagaagctc catctatctt agattatcta agcgagcctg
    34201 ctcaaaaaca ttttgatacc gttgtttcca tgctggatgc attggctgtt ccttatgaaa
    34261 tcgacagtaa tatggttcgc ggactagatt attacacaca tacgattttt gaaatcatga
    34321 gtgacgcacc aaaaatgggg gcacaagcaa cgatttgcgc aggcggacgt tatgacaatc
    34381 tagtggaaga actaggtggt ccttcaacac ctggcttcgg atttgcaatg ggtatcgagc
    34441 gattactctt gactatggag gcagaggaag tagttatccc tgcatttaat gagttggatg
    34501 cttacgtggt cgcactcgga gacgaaacaa atatcgaagc attgaaagtg gtccaagcga
    34561 ttcgaaactt tggtttttca gctgaccgcg acttcatgaa ccgtaaagca aaagcgcaat
    34621 ttaagacagc agataaagcg aatgccaaat tagtattggt gatcggtgaa gatgagcttg
    34681 ctagtcaaac gattaatgtc aaagcaatgg caagccgaaa agaaaaaagt tttccattag
    34741 aagagatata tgaacatttt gatgaagtct atgatgaaat gactttattc gagtaggagg
    34801 aaaaaaataa atggcacaaa gaacgatcta ttgcggatta gtttccgaac aacaattaga
    34861 acaaacagtt acattaaaag gatgggtaca aaaaagacga gaccttgggg gagtaatctt
    34921 tatcgaccta cgtgatcgtg aaggaatcgt tcaaatcgtc tttaatcctg aaatcaacaa
    34981 agaagcatgg gaaatcgcgg ataaatgtcg cagtgaatac gtgatcgaag tgactgggga
    35041 agttagaaga cgcggagaaa atgcagtaaa tcctaaaatg gccactggtg aattcgaagt
    35101 gattgcaagt gaaatcgtga ttttgaatca agcaaaaacc ccgccatttc aaattgaaga
    35161 tgatcaagca atcagtgacg aaatacgttt gaaatatcgt tatttggatc tgcgtcgtcc
    35221 aaaaatgact aaaaacatca aattaagaaa tgcgacaaca aaagcgattc gtcattattt
    35281 agatgataat gactttctcg atatcgaaac accttaccta gggaaatcta caccagaagg
    35341 cgcaagagac tacctagttc cttcacgtgt ccatgctgga catttctatg cgttgccgca
    35401 atctcctcaa ttgtttaaac aattgctgat gaatgctgga ttcgaccgtt attatcaaat
    35461 cgttcgttgt ttccgcgatg aagatttacg tggagaccgt cagcctgaat ttacgcaagt
    35521 agatattgaa acaacgttct taacagcaga agaaatccaa acttacacag agggattgat
    35581 tgcgaaggta atgaaagaag ttcgcggtat cgaagtcaca ttgccatttc ctcgtatgac
    35641 ctatgatgaa gcaatggctc gctacggcag cgacaaaccg gatacacgtt ttgatatgga
    35701 attgatcgat ctaagcgata cagtcaaaga agtagaattc aaagtcttcc aaatggctct
    35761 tgaaaacggc ggtgtcgtga aagcattgaa tgctaaaggc gctgcagatc gctactcacg
    35821 aaaagacatg gatcagttag gtcaatatgt aggacaattc ggagctaagg ggcttgcatg
    35881 gctgaaagta gaagaagacg gattgaaagg tccaattgct aaattcatgg gagaagcaac
    35941 agaagcaatc attaaagcaa cagatgcaaa accaggtgac ttgctgatgt ttggcgcaga
    36001 taaaagtgaa atcgtcgctg ctgcattagg tgctatccgg acacgtttag gaaaagaatt
    36061 aggactaatt gatgaatcga aattcaattt cttatgggta actgattggc cacaatttga
    36121 attttcagaa gaagaaggtc gatatgtctc tgctcaccat ccattcacga tgccaaaaga
    36181 agaagatatc ccgttattgg ctgaagaccc tgcaaaagtc tatgcagaag cttatgatat
    36241 cgtcttgaac ggttatgaat taggcggagg ttctatccgt attcatactc gtgagcttca
    36301 agaaaaagta ctagaaactc taggctttac aaaagaacaa atggaagaac aatttggttt
    36361 cttgttaaca gctttagatt acggattccc tccacatggc ggtattgcat taggattgga
    36421 ccgttttgca atgttattag ccggagaaga taatattcgt gaagtcattg cctttcctaa
    36481 aaacggaaaa gcagcagatg tgatgagtga tgcaccaagt atggtttcgc ctttacaatt
    36541 gttcgaattg aatattgatg taacgaatat agaagaatag ccgataaaaa ggtaaaaagg
    36601 atttgtcgaa tacgcttctt ttgacaacct tgatttaatt gaaaaacact ttataatgaa
    36661 gccataacat ataaagcggg gcttttccat gaaaaaaaca ttcgaaaatt gggcttatca
    36721 tgattacacg accaagcttt cggtatctat cgtttatgcg ttgatcgcat cagtcgcatt
    36781 gaatttcttt tatcagccag gacatattta ttcaagtggt attactggtc tagctcagat
    36841 tttgacgact ttgtcaaaat cactgatagg attcgaagta ccggtatcaa caactttgta
    36901 tttactgaat atacctttgt ttgtattggc gtggttaaaa atcgggaaga agtttacaat
    36961 tttcactttt atcacagtca ctttgacctc cctttgtatc catattttac cggaaacagt
    37021 cctatctacc gatccaatca tctgtgcgtt gtttgggggg gctgtgatgg gactaggtat
    37081 tgggctagcg ttgagaaacg gcctatcatc gggcggtcta gatatcgtga gtatcacgat
    37141 tcgtaaaaaa acaggaaagt cgattggttc tatttcgatc tacttcaatg cattgatcgt
    37201 ttttgttgcc ggaattctat ttggctggca atatatgttt tacagtacgt tgtctatttt
    37261 cgtcagtgga aaagtaactg atgtcgttta tactaaacaa aagaagatgc aggtaatgat
    37321 cgtaacgaaa catccagaaa aagtagtaaa tgaaatccaa aactgtatgc gtagagggat
    37381 cacgatcatc aatgaagcag aaggtgctta taagcatgat aagcagacag tattgatgac
    37441 gatcgttaca cgatacgaac tgcctctact tagagaagca atgaaagctt ctgatccaca
    37501 agcttttgta agtattgcag aaaacgtcca gatacttggt cgtttctatg aggatggcat
    37561 ttagatcgag tttgacaaaa attcatgaaa tactgctgta gacagacgtt cgtttgtctg
    37621 cagttttttt ctaccatagt agaatcagca aagaaaaact gcttttttat ttgaaatttt
    37681 cttaaagttg aaaaaaagca tgtttatttt tttaaattta tgggatgata gaaaaaaata
    37741 gtagagaaag gacacgattc tacttgctat tttcacttaa tacccgtata ttacgaatga
    37801 aaaaagttta tgaaaagttg gaatattgaa tggaaaaaca aaaaaggtta aaaaacagcc
    37861 gctacatcac tgggtttgat gggctccgtt cattagctgt ggtgggtgtt atcttctatc
    37921 accttctgcc aactagtatg aaaggcggct atttgggcgt tccgattttt ttcgtcgtat
    37981 ctggttattt gatcacggat ctgctgcggc aggaatggga acaaaatgga aaaatcaata
    38041 tttggcaatt ttatgtcaga agaatgaaga gactctatcc ggcacttgtt tttatgcttg
    38101 tgacagcttc ggcttatatc actttgtttc agcgagggct gttaaacaat cttcgaggag
    38161 ttgtggtcag cagcttactg tatgtcaaca actggtggca aatcaataac ggtttatctt
    38221 atttcgatcg atttgctaat gagtcaccgt ttacgcatat ctggtcttta gcagtagaag
    38281 gtcaaaacta tttggtttgg ccgatcttgt ttgtattgtt gatggtattt gttcgtaaaa
    38341 gaaaatggat cttttatact gtgttaggta catccttcct ctccgccatc ttgatgatga
    38401 tcttgtatac accaggaggc gatccaaccc gtgtttatta cggtaccgac actcgtctgt
    38461 tttctatatg gttgggaagt gcgcttgcgt ttgtttggcc aagtacgcgt ttaaagaaaa
    38521 acatcccgaa acaagcgaaa aatattctga atatcgctgg aggaatctcg cttttggcac
    38581 tgattctttg cttcttcttt ttagatgatc attacaattt catctattac ggaggaatgc
    38641 tcctagtgag tctgttatgc gtagtgctag ttgcagtgac agcacacccc ggagcaagtt
    38701 tgaatcgatg gctgacaaat ccagtattta cttggattgg caaacgaagc tatggcattt
    38761 atctgtatca gtttccagtt atgatttttt atgaagcaaa ggtgactgat attgccgatc
    38821 atgttttgtt gcatacttta gtggaaatca cattgattct aatcatcagt gaactttctt
    38881 atcgatatgt ggagcgtccg ctagcacgtt ttgattatgg tcgtacattc caagtgatga
    38941 aaggatggtt cagcaaaccg atcatcagta agagcaaacc atgggttgtt ccaggcacat
    39001 tacttgtagc aattgcactc gttggtttcg ttacggcacc gaaaaacgct gtaacagccg
    39061 atcaaaaaca actgcaggca aaaatcgctg aaagtaaaaa attagcagaa gaaaccagaa
    39121 acaattcaga aaacaccact agttcggttg accaagcgat ccttgataag tacgagttgt
    39181 ccaaggcaca agggaataag gcacaaaaat tagaactaac ggcatttggt gattcagtta
    39241 tgttggatgc tgcaacggat ctacaagaac tttatccaaa agtagtggta gatggagatg
    39301 tcggacgaca attatatgcc agtgtaccgt acattgaaaa attaaaggaa caagatttgt
    39361 tgaaagacac tgttctgatt ggactaggaa cgaatggctc attctcggaa gctcagtttg
    39421 atagtgtgat ggatgcaatc ggtgatcgaa aagtatactg ggtcaatgtc cgagtaccga
    39481 cgaagcgctg gcaaaatgac gtgaatgcga tgcttgaaca gatgacgaaa aaatacgaca
    39541 atatgacgct gattgattgg tacagctaca gtaatgacca tagtgactgg ttttatgatg
    39601 atcaagtcca tccaaatcct gaagggatga cacattacat tcacttagtc agcgaagcct
    39661 tgctaggagg aagttcaagt acgaacagca gtgagacaaa cgtgacagaa tgatagctac
    39721 aaaaaacttc aggcaaaaac atttgttttt gcctgaagtt tttttatatt taccgtgttt
    39781 ttaacattca tatagattca ggaagcaaaa cggttgagtg tgtctgtaaa taacgttctt
    39841 tttgttttcg ggtgagtttt ttgaatgcag cttctgcttt tgttgcttcg cttcttgaag
    39901 aaaatacttc tgcatggatc atcttaacag gtcttctttt ttgaagacga gtatatttag
    39961 caccagtacc attgttatgt tcctctaatc gtctagaaag atcggttgta tatccaccat
    40021 aaaatgtctg atctttacag aataatacgt aaaaataatg ttcatttgcc atagagcatc
    40081 tcacgaatct ctgcagtgta ttctccatcc atattgtaag tgatcaatgg tggagcgact
    40141 ttgaatccgt ctaatttgcc ttctttgatt ccttcaacta acaaaatatt tgcctctttt
    40201 ccaggttttg ggtaaacaaa ttggatcctt ttaggtgcaa ttcgatagtc ttccatgacc
    40261 tgaatgattt ctaagaaccg atcaggacga tggaccatag taaagcgtcc atttgttttt
    40321 agtagcttac ttgaaatatc gatcacttct tctaacgtcg tatgaatctc atgacgggcg
    40381 attgccaggt gtttattagg gttcgtttta tttgttggca gccctttgaa ataaggtgga
    40441 ttacagacga ctaagtcaca agaattatgt cgaacgacag ataagctgtc tttcagatcg
    40501 atggtgtgga cagtcatttg ttcatttaaa tggttaagcg cgatactacg tcttgccatg
    40561 tctgctagac gaggttgaag ttcgatagcg tcgatagccg cctgggttcg tttacttaaa
    40621 aaaagtccaa ctgcgccatt tcctgcacaa aggtcaacaa tcttcccttt tttaggtaca
    40681 cgggaaaagt gagcaagcag cacagcatct aaagaaaaag aaaacacttt ggaactttgg
    40741 ataatcttga tatcttctgc gtataattgg tcgattcgtt cgtcttcgtg aagcatggat
    40801 tccctccaaa atgaaaaaaa tagtcgtctg tattatacta tttttattaa aaagaaccaa
    40861 ggtggcttgt caaacctcta ttttttatgc atactatgac cataagaagg gaaaggaaga
    40921 gactatgttt tatcgattta tgcgcggggt agttcgagta ctattagcct tagttaatgg
    40981 aaatgcccgc tatgagaata aagaagtttt accgcaagat gagaattaca tactagttgc
    41041 tccccatcgt acttggtggg acccattata tctggcggta gctgcagcac ccaaggaatt
    41101 cagctttatg gcaaaagaag aactgttcaa aaatccagtt ctgcgattta tattaactcg
    41161 atccaatgct tttccagtta aaagagaaaa tccaggacca agcgtcatta aaacacctgt
    41221 aaagattttg aaatcgacaa atttgagttt gatcatgttt cccagtggga caagacatgc
    41281 tacagaactt aaaggcggaa tggcattgat ttccagaatg tctaaagcga aaatcgtgcc
    41341 agctgtatat caaggtccgt tgacacttgg ggatctgttc aagcgtcaga gagtgacagt
    41401 gcgtttcggc gaaccaatcg acttatcaga tatcaagaaa acggataaag aaggtatggc
    41461 ggagatcgaa cgacgaacac aagcagcttt tgacaaattg gacaaagaag tgaaccctga
    41521 tttcaaatat gaagtcaaaa aaaaataaaa tgataagttt atgcgtgggc ggtcttttga
    41581 atctactgtc ctgttcagtt cagacttttt caatgaaaac gagggagaga cgtcaagtct
    41641 gccctctttt tgcgttccaa tatgtaaaat agaagacagg tgtcatgtaa tggatcaaag
    41701 tgttataata attagaagga cattttttga ggtgattgaa tgcgtttttt acatacagcc
    41761 gattggcata tcggaaaaaa actacaaggt tatgacttga ttgaagacca gcgtcatgta
    41821 ctatcttcta tactagatat cgcgaaagaa gaaaaagtag aggcaatcgt gattgccgga
    41881 gacttatatg ataggagtgt cccagctgtt gatgccgtag aattatacaa tgaattgatg
    41941 gtcgattgga atttaaagga aaagatcccg atttttgcta tatcaggaaa tcacgatagc
    42001 agtacgagac tttcagcggg tacgccatgg ttcagttatt cgaactattt tttgcgaaca
    42061 cagctagcag aagcatttga accagtcgag tttaaaaacg tacaattttt ctttcttcct
    42121 tactttgaac caattgcagc gagattatat tttgaagatg acaatatccg aacgatcaag
    42181 caagcggtag ccaaggtgat agataaaatg caggagctat ttgattcaga aaaaaaacaa
    42241 gtattgatta gccatttttt tgtaatggga agtgtaaaat ctgattcgga aacgaaaatc
    42301 gaagtaggcg ggttggattc gatccctggt gaactactta caccgtttga ttatgtagca
    42361 ctaggacatt tgcataatca agctgctttg agacaggata atgctcgcta tagcggttcc
    42421 ccattgaaat tctctttatc tgagatcaac caaaaaaaag gcgtatggat cgttgacctg
    42481 acaacaggcc tggagttgaa ttttagggag atcgaacccc tgcatgatat tgaagaaata
    42541 acagcgagtt tcaaagaatt gctggatcct gccttttatc agtccgtcaa tcgggagaat
    42601 tacatctata tctatctcac tgatagaacg atcatcccga atatgatgaa ccagcttcgg
    42661 caaatatatc ctcgtatttt ggggattgaa cgggcaaacg gtcgagcaga acaaacaaag
    42721 cgcaaacgaa aagtactaga aatgaaagat cctcaaaaat tagcagaagc attttttagt
    42781 gaaatgacgg aagagcagct aactgcaaac caagaaaaat ggctcggaga aacgttgcaa
    42841 gaaatcatgg aaatggaatg atcaagaatg aaaccgaaaa aattacaact taaaaatttt
    42901 ggtcctttta tcaatgagac cgtagatttt tcacgtttga cagaagcacc gctttttttg
    42961 atcagcggga agacaggagc tggcaaaacg acgatttttg atgggatcac cttcgcactt
    43021 tttggtgaaa cttccggaag acttcgctct ggaaaagaga tgcgttcttt gtttgctaca
    43081 ccagacgaag aaacaagcgt gactttttct tttgaacatc agcagatgag ctatgaaatc
    43141 gaacgaaaac cagaacaagt tttagcaaaa cgaaaaggaa acggaacaaa aaaacaggca
    43201 gccaaagtaa cgttgaccat ttttgatggg aaaggaaaag aaataaaaca aatcacaaaa
    43261 cgtacagaag cagatcagtt gatcagagaa ctgatgaatc tcgatgcgaa acagttttct
    43321 caaatcgttt tactacccca aggagaattc cgtaattttt tggtttcatc aagcagtgat
    43381 aaagagactg ttttacgtca tttattcggt actcaatttt tccaacagtt caatgagcga
    43441 ttaaaagaaa aagcaaaaca acagcaacaa aaattggatc atttagagca agagctgcat
    43501 cttctgcaaa aaaggtttgt tttagtcaaa gaagaggaac cagcagcagg gtttgaagcc
    43561 gttttagccc aatggacaaa ccatcaggca atactcgaaa aacaaatcac ggaagaaaaa
    43621 gaagctctaa cggaacttca ggaaaaacaa aagcaactag aaaattccta ctatcgtttt
    43681 gaaagcttga aaaaaagtta tgaagaaaaa acgatattaa taaacaagca aaaggaatta
    43741 gctgctcaaa aagacgaaat agaggaaaag aaacgatgga ttgggtatta tgaatttacg
    43801 caacgattag tcgaaccaat ccagcgccta caagaaataa aagttgaaaa aatagaactg
    43861 tcacaaaaaa gtagtgagca aacaaagaaa ctagcgatga ctacgcagaa ttatgaagag
    43921 tggcaaaaaa atgaagcaaa ccgcgtacgc cgacaaacaa aaatcaaaga agctgctcaa
    43981 aaactgcaaa acaaacagat gatggtgcca attgtcgaat ccttacgtag taaaaaacaa
    44041 gacgcagaac aattaacaat ttcattggat aaagaagaat tgaatgccga gaagctgtat
    44101 caaaatctcg ctatctatca ggaaagaact cgaattcttc aagaaaattt actggatcag
    44161 gaaaaactgc aagaagatcg tcttgctttt ggacagttaa aatatctaga tgaaaagcag
    44221 caagaaatag aaaaacaaaa agcaaaagac actgggtcgc tcgcacagat aagagaagaa
    44281 ctcaaaaaag caactgaaga aataacaaaa aatagtcagc tattgaaaga acagacagaa
    44341 caagcagaac agctgaaaag ccaatgggca aagcagcaaa ttgctcgtct tcagcttttt
    44401 ttgcgtccag gggagccatg tcttgtttgt ggatcaacgg aacaccccat acaacaatta
    44461 aaacacgaac agccaaataa ggaagatatc ttaaaagtag aacaacaact ggcagaagct
    44521 gagtctgctg tagaaagaac tcagcaaatc attgccaaaa gtgaagcaca tgaaaataat
    44581 cttcaacaac aattatcaga tttagaacaa aaaatcatgg caacgaatat acaattggct
    44641 gaacaagaag aacacttgca acaggctttg aaaaaggcat ttcctgagat aacagaacaa
    44701 aaaacaaaaa caactcttgt agtaatcgaa caatctttag atagacgaaa aagcaagatg
    44761 gccaatgaaa gaatagagct ggaagaaata agacaacaag cagaaaagct cgaacaacaa
    44821 ttagctgtct acaaacagca gcagacagaa aaaaataatc agctccagca actgcaagga
    44881 gaaatcaaaa gtttagaaca acaattgcaa gatacggata gtaactgttt agaagaggag
    44941 atacgacgac ttgaaaaaca gttagaagaa gactcgaatc aactagctgt tgagcagaaa
    45001 gcaggagtgc aattgttgca agatcttcat acgcttcgga cacagcttga tcttcaacat
    45061 gaacaaatag aaaaaataga tgaaaaaata gaaaaggaac aagaaagaat agctaaaaag
    45121 ttagaaagcc agtcgtattt ttcaacaatt gaagaaatct gtgcatttgc acaacagcaa
    45181 gcacaatata aagaaaacca acgtacagtt acttcttatc atgaagagca attagtgaca
    45241 gctgaccggc tgcgtcaatt agaaaatatc cagctaactg agacattccc agatacagaa
    45301 gagatcagcc aacagttaga agaaataaga cgtatgatcg aaataaagca agaaaagata
    45361 tacggtttac aagaacaaaa aattagtaat caaaaaatct acgatgaatg tctttcgatt
    45421 tatcacacaa gccagacgaa attagatgag cttacgcaat tacagcagct ttcgcaaaca
    45481 ttcaacggag aaaatcctaa gaagactagt ttggaacggt atgtgcttca ggtttatctt
    45541 caagaagtgt tacaagtagc aaatgatcat ctccaacgtt taacgaaaaa tcgttatcaa
    45601 tttgagctag cggataccat tggcagttat cgagggaaaa ccggattaga aatcaatatc
    45661 tatgatgatc aggcaggtat gtctcgaagt gcgcataccc tgtctggcgg tgagagtttt
    45721 attgcagcac tttctttagc attagctctt gccgatgtga tccagacaca agctggtggt
    45781 gtagcaatcg atgcattgtt tattgatgaa ggatttgggt cattagatga agaagctttg
    45841 gaaatggcaa tggaagcttt agaaacgatc gaaagtgaag gaagaatgat cggcatcatt
    45901 agtcatgtaa gagaattgaa agaacgagta ttacaacaaa taagaattga tacagaaggt
    45961 agcggacaaa gcaaaatacg ctatcaactt ggataaaaag ggagattggt aactatgaaa
    46021 tggagtattc cagaaaaagt cattgcacgt ggacgtcaat acttgcaaga aggccgagtg
    46081 ttatcggttg tccctgacca agaagaaaac gtgtggcatg cagaagtctt gggcagtgaa
    46141 ttgtatatgg ttgatttgga tggtacagca aaagagatag attactgcca atgtccctat
    46201 tgggaggaac ataaatattg taagcataca gttgctgtgg aactttattt acggtcgaaa
    46261 aatctatctc gtatcatgaa aaaagaccag ccaatgctag aaccggtaaa agcaacaagt
    46321 gagggagaaa tcattgcaaa aggtttttct cgtctaaaaa cacaaaacaa tcctcaagcg
    46381 gtgcaacctt tgattattga ataccaagta gaaacgattg agactaatca gtatcaccca
    46441 gagctttcta ttttggcact ttacttgcgt atcggttcat tggcatcacc gaaaaaaaca
    46501 tatatcgtaa aaaacatcta tgatttttta caagcttaca cagaaaaaga aacttacact
    46561 gttaataaac agtaccagtt tcgtctagat aaacgggcgt ttcaaaaaga agatcaaaaa
    46621 atactcacgc atcttgcagg aagtgctcag acgcatcaat tattaggtac gaacggctta
    46681 caagtcaaag gaaaactaga taaaagatat ctgcttctcc cagtagaaca agcccgcttc
    46741 ctgttagagc agatgaatca gactacacgt ttgcatctgc agataaatga acaaaacgat
    46801 caaggaatac aattttctga tcaaaacaaa cctttatcat tcaaagtgga aaaatttgga
    46861 gaagattatt tgctgcaagc attgaatgat tttgattttt tctttatgca ttatcagtgg
    46921 ggagttatcc aagggacatt ttattctctg acgaaatatc aacaaacggt ttatcaaact
    46981 tggaaacaat tgattcgccg attggagaaa ccagaagtag tgttcaagaa aaaagaatta
    47041 ccgtctttgt ttaaagagat tatccctctg ctgtcagaga tcggcgacgt agctattgcc
    47101 cctgcagtag aagaggaagt agcagatatc cccgtcgaat ttattttctt tttccgaaaa
    47161 gcaaaaggaa aaatccaagc tcgagtcgat ttcgtctatg gtgaagtcat ctattcgact
    47221 gacccgaagc atgaggtctc gacagaacag gcttttcagg tgttacgtga cacagtagaa
    47281 gagaaacgag tacttgatct attcagaatg tatcgctacc agaaaaatga aactggttat
    47341 gaacgtccat tgccaagcgg ggaagaacta tatgcctttt tccgcacaga actatctgtc
    47401 ttccgtcagt ttggtgaagt cagacttgga agaaaattgc gagagttgta tttagatgcg
    47461 cataaacatc aaccgaaaat cgaagtaacc gagaccggtt catggctcga tattcgattc
    47521 gatgtgacag ggatagagga gcaagaaatc gatcatgtcc tgcaaagctt gctgagaaat
    47581 gatgcttttt atacattaga aaatggtcag gttctttctt ttgattcaga agaatttcaa
    47641 caaacaagcc aagtgttgca acaattacgg gaatcgatac gtacagaaga aggaacgatc
    47701 catgtaccta aaaatcaagg attgatcatt caaaatcaat tggaaaaaag caatgctact
    47761 tttagtgaat cgttccaaac gatggtacaa gatctcattc atccagaaag ataccaggca
    47821 cagcttccca aaggattgaa tgcaacgatg agagattatc aaaagcaagg ttttcgatgg
    47881 ttgaagatgt taggtcatta tcagtttggc ggtattttag ccgatgaaat gggacttggg
    47941 aaaacactac agacaattgc ctttttactt tcggaaaaag aagaacggaa aagcttctct
    48001 gctttgatcg tagcgcctgc gagcttaatt tacaactggc aggcagaggt gagaaaattc
    48061 gccccttctt taagtatcca agtaatcaat gggaataaaa aagaacgtga agaattactg
    48121 gctaaagaca cagatatccg agtgacttct tatgccagct taagacagga tctagctaat
    48181 tatcagtcgc aaaaaattga ttatttgatt cttgatgagg cacaaatggt caaaaatagc
    48241 agtacgaaaa ctgcacaagc attgagagaa ttagctgtac ctcagcggtt tgcactgagt
    48301 gggacaccaa tcgagaataa tttagaagaa ttgtggtcat tgtttgcaac gattatgcca
    48361 ggatttttcc cgactaaaac aaaattcagg gaacttgccg cagaagaaat tgcgcaaatg
    48421 attcgaccgt ttattttacg gcgagataaa caaactgtgt tgaaagactt gccggaaaaa
    48481 acagagatga atctatattc tgcacttaca gaagaacaaa aaactgttta tttagcttac
    48541 ttgcgtcaaa tgcgtgaaga aatcacatca atggattctg aagcattcaa gaaaaaccgg
    48601 atcgggatac ttgctggtct gacaagactg cgacaaattt gctgcgatcc tcgtctgttt
    48661 attgaagatt accaaggcgg ttccggcaag ctcgaacaag taaaagatct actggtagct
    48721 gccaaagaga acaaacgaag agttttgctg ttttctcaat ttacagggat gctgactatc
    48781 ttgcaagaag agttagccga gctagggatc tcgacatttt atttaagagg cagtacgaaa
    48841 ccacaagatc ggttaagtat ggtagacgca tttaatgctg gcgaaaaaga tgtttttctg
    48901 atttccttaa aggctggggg aactggattg aatctcacgg gagcagatac ggtcatcttg
    48961 tatgaccttt ggtggaatcc tgccattgaa gaacaagctg ctggacgtgc acatcggatc
    49021 ggacaaaaaa atgtggtaga agtgtggcgt atgatctctg aaggaacgat tgaggaacgc
    49081 atggattcac tccagcaaga aaaacgcgaa ctgttccaaa aagtgatcca aggtaatgaa
    49141 gaacagttga cgaaactgac tgaagaagac atccgcatga ttttaagtgt aggagaaatc
    49201 gaggagtaag agactcgaaa aagcgttcag taatgggtaa gaaagccttt ctaaagaagc
    49261 gattgtttat ttgatttaaa aaatagcaag gaggctggga ttccatttta tcccagcttc
    49321 cttgctatca tttttttatt tctattttta aaggttatac aaagattgtc ttcattgtct
    49381 ctaacagctg atcaaaagaa gaaatctcga tatcgcaaat tggagtctgt tcaattttgc
    49441 gttctcgatg gttgaaccaa agcgcttgcc agttagcact ttttgcacct agaacatcat
    49501 tgtcgtaatt atcgccaacg tataaggtct cttctggaag catatgaaat tctttctcag
    49561 ccagctgaaa aatctctttt tctggttttt ggaaacctgt tgcttgagaa atgagcatat
    49621 tgcgggaagg aatccaattg ttcaattgaa gttgcttcag tttttttgtc tgatggtcag
    49681 tcggcccatt tgtgataatt cctaatggga cattcttttc cttcaagaaa tcgagggttt
    49741 ttttgacctc ctcatgcaag cagatattat caagttcttc ctcgtaaatt ttttgaaata
    49801 agaggccatc ttcttccgta atatgagggt agtctaaatc ttttagtgat tggctgatcc
    49861 gatgtgctcg catatattct agtgtccaat ctcctgccat tacttttggg aagttttcat
    49921 cactatgatg gcggaaccgg atatatagtg gatgcatatc tgcatctgaa acaagcggaa
    49981 cgactctttt gaccgcgttt cgaaatggct gctgctgatc atacatcgta tcatcaacat
    50041 caaacacaac tgatttaatc aaaattttac acgcctttcg gttttcattg attttttcac
    50101 aaatccttga ctattgtaac agaaaaaatt gtctaaaaaa gctgataaat aaaggttttt
    50161 cttactatat ttctatttcc ttgacagaat aaggttttta tatttcagtt tttttcacta
    50221 aaaatcgtga aacacgtcaa taagtttgat gaacaaatta atcattatgg
//
LOCUS       DARBDV010000009        46111 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000009 DARBDV010000000
VERSION     DARBDV010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.009"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(19..1122)
                     /locus_tag="SCM56_000531"
     CDS             complement(19..1122)
                     /locus_tag="SCM56_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEG1109358.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(1751..2221)
                     /locus_tag="SCM56_000532"
     CDS             complement(1751..2221)
                     /locus_tag="SCM56_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HEG1109359.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            2551..4191
                     /locus_tag="SCM56_000533"
     CDS             2551..4191
                     /locus_tag="SCM56_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEG1109360.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            4345..5667
                     /locus_tag="SCM56_000534"
     CDS             4345..5667
                     /locus_tag="SCM56_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HEG1109361.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(5735..6169)
                     /locus_tag="SCM56_000535"
     CDS             complement(5735..6169)
                     /locus_tag="SCM56_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1109362.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(6234..7071)
                     /locus_tag="SCM56_000536"
                     /pseudo
     CDS             complement(6234..7071)
                     /locus_tag="SCM56_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            complement(7813..8439)
                     /locus_tag="SCM56_000537"
     CDS             complement(7813..8439)
                     /locus_tag="SCM56_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109363.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(8734..9069)
                     /locus_tag="SCM56_000538"
     CDS             complement(8734..9069)
                     /locus_tag="SCM56_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEG1109364.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(9056..9340)
                     /locus_tag="SCM56_000539"
     CDS             complement(9056..9340)
                     /locus_tag="SCM56_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEG1109365.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(9396..10919)
                     /locus_tag="SCM56_000540"
     CDS             complement(9396..10919)
                     /locus_tag="SCM56_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEG1109366.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(10912..12087)
                     /locus_tag="SCM56_000541"
     CDS             complement(10912..12087)
                     /locus_tag="SCM56_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEG1109367.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(12091..12276)
                     /locus_tag="SCM56_000542"
     CDS             complement(12091..12276)
                     /locus_tag="SCM56_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109368.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(12242..13936)
                     /locus_tag="SCM56_000543"
     CDS             complement(12242..13936)
                     /locus_tag="SCM56_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEG1109369.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(13933..14406)
                     /locus_tag="SCM56_000544"
     CDS             complement(13933..14406)
                     /locus_tag="SCM56_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HEG1109370.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(14475..14630)
                     /locus_tag="SCM56_000545"
     CDS             complement(14475..14630)
                     /locus_tag="SCM56_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HEG1109371.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(14764..15144)
                     /locus_tag="SCM56_000546"
     CDS             complement(14764..15144)
                     /locus_tag="SCM56_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEG1109372.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(15148..15363)
                     /locus_tag="SCM56_000547"
     CDS             complement(15148..15363)
                     /locus_tag="SCM56_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109373.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(15366..15773)
                     /locus_tag="SCM56_000548"
     CDS             complement(15366..15773)
                     /locus_tag="SCM56_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HEG1109374.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(16039..17514)
                     /locus_tag="SCM56_000549"
     CDS             complement(16039..17514)
                     /locus_tag="SCM56_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEG1109375.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(17504..18352)
                     /locus_tag="SCM56_000550"
     CDS             complement(17504..18352)
                     /locus_tag="SCM56_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HEG1109376.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(18389..18751)
                     /locus_tag="SCM56_000551"
     CDS             complement(18389..18751)
                     /locus_tag="SCM56_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109377.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(18752..18910)
                     /locus_tag="SCM56_000552"
     CDS             complement(18752..18910)
                     /locus_tag="SCM56_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109378.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(19051..19675)
                     /locus_tag="SCM56_000553"
                     /pseudo
     CDS             complement(19051..19675)
                     /locus_tag="SCM56_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(19672..19983)
                     /locus_tag="SCM56_000554"
     CDS             complement(19672..19983)
                     /locus_tag="SCM56_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109379.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(20027..20320)
                     /locus_tag="SCM56_000555"
     CDS             complement(20027..20320)
                     /locus_tag="SCM56_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109380.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            20514..21161
                     /locus_tag="SCM56_000556"
     CDS             20514..21161
                     /locus_tag="SCM56_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1109381.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            21222..22367
                     /locus_tag="SCM56_000557"
     CDS             21222..22367
                     /locus_tag="SCM56_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1109382.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(22448..22753)
                     /locus_tag="SCM56_000558"
     CDS             complement(22448..22753)
                     /locus_tag="SCM56_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEG1109383.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(22778..23209)
                     /gene="ruvX"
                     /locus_tag="SCM56_000559"
     CDS             complement(22778..23209)
                     /gene="ruvX"
                     /locus_tag="SCM56_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEG1109384.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(23206..23472)
                     /locus_tag="SCM56_000560"
     CDS             complement(23206..23472)
                     /locus_tag="SCM56_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEG1109385.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(23727..24452)
                     /locus_tag="SCM56_000561"
     CDS             complement(23727..24452)
                     /locus_tag="SCM56_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEG1109386.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(24813..24883)
                     /locus_tag="SCM56_000562"
     tRNA            complement(24813..24883)
                     /locus_tag="SCM56_000562"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24849..24851),aa:Gly,seq:ccc)
     gene            25000..25752
                     /locus_tag="SCM56_000563"
     CDS             25000..25752
                     /locus_tag="SCM56_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEG1109387.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            25768..26247
                     /locus_tag="SCM56_000564"
     CDS             25768..26247
                     /locus_tag="SCM56_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEG1109388.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(26498..28078)
                     /locus_tag="SCM56_000565"
     CDS             complement(26498..28078)
                     /locus_tag="SCM56_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEG1109389.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(28071..28814)
                     /locus_tag="SCM56_000566"
     CDS             complement(28071..28814)
                     /locus_tag="SCM56_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1109390.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(28990..29766)
                     /locus_tag="SCM56_000567"
     CDS             complement(28990..29766)
                     /locus_tag="SCM56_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1109391.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(30014..30850)
                     /locus_tag="SCM56_000568"
     CDS             complement(30014..30850)
                     /locus_tag="SCM56_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEG1109392.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(30861..31790)
                     /locus_tag="SCM56_000569"
     CDS             complement(30861..31790)
                     /locus_tag="SCM56_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEG1109393.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            31982..32335
                     /locus_tag="SCM56_000570"
     CDS             31982..32335
                     /locus_tag="SCM56_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEG1109394.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(32409..33065)
                     /locus_tag="SCM56_000571"
     CDS             complement(32409..33065)
                     /locus_tag="SCM56_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEG1109395.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            33291..35225
                     /locus_tag="SCM56_000572"
     CDS             33291..35225
                     /locus_tag="SCM56_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEG1109396.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(35311..36534)
                     /locus_tag="SCM56_000573"
     CDS             complement(35311..36534)
                     /locus_tag="SCM56_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEG1109397.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(36528..38426)
                     /locus_tag="SCM56_000574"
     CDS             complement(36528..38426)
                     /locus_tag="SCM56_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HEG1109398.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(38420..39538)
                     /locus_tag="SCM56_000575"
     CDS             complement(38420..39538)
                     /locus_tag="SCM56_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEG1109399.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(39621..40454)
                     /locus_tag="SCM56_000576"
     CDS             complement(39621..40454)
                     /locus_tag="SCM56_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1109400.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(40447..41364)
                     /locus_tag="SCM56_000577"
     CDS             complement(40447..41364)
                     /locus_tag="SCM56_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1109401.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(41591..42715)
                     /locus_tag="SCM56_000578"
     CDS             complement(41591..42715)
                     /locus_tag="SCM56_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEG1109402.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            42898..43806
                     /locus_tag="SCM56_000579"
     CDS             42898..43806
                     /locus_tag="SCM56_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1109403.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            43947..45560
                     /locus_tag="SCM56_000580"
     CDS             43947..45560
                     /locus_tag="SCM56_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEG1109404.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT        13915 a         9230 c         7685 g        15281 t
ORIGIN      
        1 tttttcgtca tttatttctt aaataaaacg attctcacgc aattttattt ttttgatatc
       61 ttctactgtt tgtgttccag ctaattgcat gaccatttcc aattctgttt taaagaaatc
      121 aaacacttga tggactcctg tacttcctcc taaagacaat ccataaataa cgggtctgcc
      181 aatagcaaca agatcggcac ctgatgcgat tgctttgaaa acatgttgtc cacgtcgtac
      241 accgctgtca aacacgattg gtacacgttt atctactgct tctgccacat attgcaacga
      301 atcaaacgca gcaggccccc catctaattg acggccacca tggttagata cccaaattcc
      361 accagcacca gattctaatg atcgatagac atcttcttct gattgaacac ctttcacgta
      421 gactggcaaa tcagaatgag cagcgataaa ttctacatct tttggactaa gtttttgttt
      481 ggaagattta tatacagcat ccatcgtttg gcctactcct gattgataag cctgaacgat
      541 cggcattggc agcgggaagg taaacccgtt tctgcggtct gtttcacggt ttccgccaac
      601 tgttgcatcc gctgtcaata cgattgcttt tgctccatta cgcttggcca cttcaagaat
      661 atccaaattg attccattat ctttgctcat ataaaattga aaccattgcg gtgctttctc
      721 cccgcctgct tctcttatct cttctaaagt acaagaagcg taagaacttg ctgtatagat
      781 cgtcccaaaa tctgctacac cttttgcaga agctttctcc gccttcacat gagctaatcc
      841 atgagcagca actggtgcca tgatgattgg tgcagtcagc atttcttcgt caaaatgtgt
      901 agttgtatcg ggtaattcta catctcttaa tacatgagga ataatcaaac gatgattgaa
      961 tgcccgttca ttttcttgat aagtaaataa gtcgccagca ccactgctga tgtaaccata
     1021 accaccagct ggaatcactt gtgaagccgc gatttctaaa tcttccatat tgataaaatc
     1081 tactattcct tcatttgtac ctgcttgata tgttttttcc atctcaatgc cttctctcta
     1141 taaaatttga atgatgaaca atatgatagc gtttacactt tttatattac tcttcgacat
     1201 tgatacaggc aaataatctg tttacatttc agccattcca gatgtcacta ctctggaaga
     1261 aatgattgta ataaaaatag ataaaaaacc gatgggactt tgacaaaagt agtgtcaaag
     1321 tccctttttt tgaataaacg gtgttcaaaa gccattttct cggtattaag tcaaatttat
     1381 gcaaaaatgt ggaaagccat tttcacaaaa tttttcttac tactcgaaac taagcgactt
     1441 tttcacaacc tctcgataaa acggtgttca atcagctgac cctcggtatt agctcaaaaa
     1501 atggcaaaat atgaggaact attttgccat ttgtctctct aatactcagg tcagaacgtt
     1561 gacttcacag tctcttcctt aaacggtgtt caaaaacttg catctgcggt aataagccca
     1621 accaaacgaa aaataggagg agctattttt gtttggctgt tcttattact tgatgcaggg
     1681 caggtttttc acgacctctg attcacggtg tttctttttc atcaacattt ttacgaaata
     1741 ctgtggaaga ctattgaaat aagtgtggat tgtttctttg tgtttcaatc gtttcttgcc
     1801 attcttcatt taattttgga ttaccgtttt cttccttgaa ctcataaggt cctaattcaa
     1861 aaatcccaga cagtatttga ttcacgattt catatagttc agtgtgatca gacacatgat
     1921 tcttgaagat catctcatta tcaccacgtt tgaacctcac attcgggctc tcatgatgaa
     1981 cctctatgca tttttctcct ttttttaact gacggacttc tgaaaacatc tcataaatat
     2041 cttctccagt aaaaccttca gcaagcgcca gactgtccac aagaccgcct actgtcagtt
     2101 cagagattat tgcttgattg gaatctaatt ttaatgtata gcttgtcgct tctactacgc
     2161 cacatgcatt gcaaccgcct tctgatagtt cgctttttct aatcaattga acttctttca
     2221 ttttctcttc ctcctcttaa ttttctactt atgtattttt atcacctgac ataggtgact
     2281 cctatcaacg ctatcatgtg cttttttcct cagaaatcct atttatccat tccatctatc
     2341 ccttattctt ctacttttct ttctctagca tataagagat acgcatagta ttcaagcaag
     2401 gattgtcgat ataaaaagaa caaatactgc tattttgatt tcttttggct atattacata
     2461 taaataaaat ttatcatacc tgctcttttt gactatatca tcatctgact tctatacttc
     2521 gtttgtatta aaacttagga ggaatcattc atgcttacag ggttaaactt attaaataat
     2581 ccttttctaa ataaaggaac agcttttaca aaagaagaac gggcaaaata tggaatcact
     2641 ggtatgcttc caagtactgt ccaaacactg gaacaacaat ctgttcaagc ttatggtcaa
     2701 tacttaagca aacaaacaga tttggaaaaa cgtatcttct taatgaatct attcaatacg
     2761 aatcggacat tgttttataa attgatgggc gaacatttag ttgaattcat gcctgtcgtt
     2821 tatgatcctg tcgttgcgga ttcaatcgaa caatacaacg aaattttctt gaaaccgcaa
     2881 gatgctgcat ttctttcaat cgacgaacca gaaaacatca aagcaagttt gaagaacgcg
     2941 gcagatggac gagacattcg tttgattgtt gtgactgatg cagaaggaat cttaggtatg
     3001 ggcgactggg gtgtgaacgg tgttgacatc gctatcggca aattgatggt ctatactgct
     3061 gctgctggta ttaatccagc acaagttttg cctgtatcga tcgacgctgg tactaataat
     3121 gaagaattat tgaataatcc cttatactta ggcaatcgtc atgctcgtgt agaaggcgaa
     3181 acttactatg aattcatcga tcaattcgtt caatcagcta ccgaattatt cccagaattg
     3241 ttattgcatt gggaagattt tggacgagga aatgctgcag cgatcttaga aaaatacgaa
     3301 gatcaaatca ctactttcaa cgacgatatc caaggtacag gaattgttgt cctagcaggt
     3361 gtattaggcg gattgaatat atccggtgaa caattgaaag atcaaataat tttgacattt
     3421 ggtgctggta ctgctggtgt gggtatcgca aacatccttt tagatgagat gatccgtcaa
     3481 ggtgtacctg aagaagaagc acgccaacat ttctatcaag tagataaaca aggtttattg
     3541 tttgaagata cagaaggatt gactcctggt caaatcccat tcgctcgcaa acgctcagat
     3601 ttcactaatg ctgatgaatt gacaaactta gaagcagttg taaaagcgat ccatccgaca
     3661 atcatgattg gtacttctac ccaacctgga gcattcactg aagaaatcgt gaaagaaatg
     3721 gctgctcata caccacgtcc gatcatcatg cctttgtcta atccaacaaa attggcagaa
     3781 gcaaaagcaa aagatttaat agaatggacg gacggaaaag cattagttgg aacaggtatc
     3841 cctgctgctg atgttgaata taatggtgta acttaccaaa tcggtcaagc aaataatgct
     3901 ttgatgtacc caggtttagg acttggtttg attgcttcaa cagctacacg agtaaatgct
     3961 gaaatcattt ctcaagctag tcgagcatta ggcggaatcg ttgatgtaac aaaaccagga
     4021 gcagctatct tgcctccagt agcaaaaatc actgaatttt ctcaaacgat tgctgaaact
     4081 gtcgcaaaat cagttgtcgc acaaaacttg aatcgtgaag aaatcacgga tatcaaagaa
     4141 gcagttgaat ctgctaaatg ggttcctgaa tataaatctt tagaagatta agatcgttat
     4201 agtaaaagaa catacagaaa ctttgtatga cgtctaaaaa gaaaaaagcg actgcggcaa
     4261 aacagcggcc agccaactcc tgctcttttg tcgcttttct ttggcttttt tcatatgata
     4321 cagaataaga aaggatctgt aaaaatggca caaaaaacaa caactgcaac tgcagttgag
     4381 gaaaagaaaa aagaaggttt ttggtccaca aaaatcaccg gcgtaagttt gccactttat
     4441 ttgatcatgg tccttgtcct gatcattgca atggctttag ggaaattacc gacgaatatg
     4501 attggaccaa tcgcagtttt ggttattttc ggtaatttat ttcattttat cgggacaaaa
     4561 atccctatcg tcaaaagtta tttgggtggt ggttccgttt ttgcgatctt cgcttctgct
     4621 gcgattgcta catttgggat cttacctgaa tatgtagtga aatccacaga aaactttgtc
     4681 agcaatatgg gcttttggga cttttatatt gctgccttga ttactggtag catccttggt
     4741 atgaaccgta atctattgat gaaagcttct gttcgtttta ttcccgttgc acttatttca
     4801 atggtcgtct ccttttttgc cgtaggactc gtgggcatgc ttatcggaaa tggttttgca
     4861 aattccgttt tatacatctc attacctgcg atggctggtg gtgttggagc cggagccgtt
     4921 cctttatcaa cgatttatgc aaatgtttta ggtacggatg cttcttctat catttcccgc
     4981 ctgattcctg cgacagcaat gacaaatgtc ttagctatca tcggtgcagc attagttgct
     5041 cgtgctggac aatcaatgcc aaaattcaac ggcaatggta aattaatgga aaaaggcgat
     5101 cttggcgatg gaaaacctcg aactgtgaaa ccttccattc cacagttagg tgttggtttg
     5161 atggtatcat tgactttctt catcttagga caaattggta actattttgt tcctaaagtc
     5221 catgcttacg catttatgat tattatcgtt gtcatctgta aaatcgccaa cctattacca
     5281 gagtattatg aagatgcagc tatcatgttc aataacttga ttgtcaaaaa cttgacagca
     5341 gctgttcttg ccggtatcgg tattgcgttg ctgaatctga atgtattagc ttctgctttg
     5401 acatggcaat ttgttgtctt atgtctgaca agtgttatcg cgatttcagt tgtttcagga
     5461 tttgtcggtc gtttgtttgg tttatatcca attgaatcaa ctattactgc tggcttatgc
     5521 aacaactcga tgggcggaac aggaaatgta gccgtcttat ctgcagctaa tcggatggaa
     5581 cttattgctt ttgctcaaat gggcaatcga cttggtggtg ccatcgtttt gatcatctct
     5641 ggtttcttga tgcaattact ttcttagtca aaaaaacaaa aatacctgat catcaagtat
     5701 cggcttggtg atcgggtatt tttttgataa agatttacag gattttcaaa aatctttttt
     5761 gcattttatc ggaatgataa ccgatgtgtt cataaaattg gtgtgcttct actctgtatt
     5821 ctgcagaatt aagccttatt gtcgttattc ctcgtttttt cgcttcttct tctacgccag
     5881 tcatcaaagc ctttccgatg cctctttttt cagcatattt agacacagca agtgccataa
     5941 tattgaacat tggatcgcta aaaatcgatt cataggactc cgcttgtaca tagccagctg
     6001 cttttttaga agtaggatct tcataaacca agaaaaaatt tttctctggc tccttcagca
     6061 gtttcttcat ttgtctcacc gtaacggtta atggtatatc ataccctaat tgatccgcat
     6121 tgatttcacg tagtgcttct gcatcttcta aacgcatttc ccgtaacatc caaatcacca
     6181 gccaatttct gattttttaa aatgaatttt tttatcatag tatactcttt ttttcattta
     6241 gaacaatatg tttcagtgat tttgacttgc gttagtctag attctcctaa tgtttgtaag
     6301 atgacaaagg cattgtaaca attgaccaaa agaaccacga ggacaattac ttgaaaaata
     6361 aaggtcactt tttttggaga caaaaccctg ctaaatcttg ctccccacaa acctccaata
     6421 acagcagcgg ggataacata aaacaacata gataaatcaa agcgcataaa gctacttgtt
     6481 aaagcaatcg ttaccaattt tgccagttga gaaaagaaaa tcgtagatag agaatatagt
     6541 gttgcttctt ttattggcaa tgcaaacatc agcatcaaca aagaaacatt gactggtcca
     6601 ccaccaatac ctaaaaaact agctaaaaag cccagaaccc cccccacata taaggtacca
     6661 agtccatgac gtcaaatgaa attttggctg atgatgcttg gtgtagaaga acgcaaaaac
     6721 caaggtcaat accgtcaaaa aaatttggat cagctgcaca tgcttctcgt tttcaaataa
     6781 ctgtaaaaaa acttcgaata caatgttgcc gactactcct ccaacaacag ctccactaga
     6841 tacccaaccg acaatctgcc aattcagtga tttcccgctt gatacttgtc gcacagtcga
     6901 aacaatggac atcgtgaata ccgcaactgt tgagtaaaaa gaaatccctg caacagaatg
     6961 ggcgccgata agatcaagaa tcggtttgat caacacgcct ccacccatcc cggaaatagc
     7021 gccaactgtg ttcgccagaa cgatcaccac aaaataaatc agaccaatca tcttgtattt
     7081 cctttctctt gtttatatct tttagcgatt gttactcttt tcttttatta tacagtaaaa
     7141 gaaacaaacg aggcttcgaa agcttttcta ctttttaagg aaatttagtg ctaaacgaaa
     7201 agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca aaaatagctc
     7261 ctcataattt tcgtttggtt gaacttatta ccgcagatgc aagcttttga acaccgttta
     7321 agaaagaggt cgtgagcctg ccgttcagct ccgagtcaca aggaacaatt tcaaaaaaca
     7381 gcttctcatg tttgttgaat attgtgactt gtgaccgagg agttggcagg agaacaccgt
     7441 ttaaggaaga gactgtgaag tcaacgtttt gacctgatta ttagagaaac aaatggcaaa
     7501 atagttcttc atatttcgcc gtttttcgag cgaatgccga ggggcagctg attgaacatc
     7561 gtttatttgg aaaaagggat tgtgacaaga cgtttgtcac aatccctttt tttatatctt
     7621 attaattcat tcttttgttc tattcttctt cttccgccat gaatgagaaa cccatatcaa
     7681 agaactataa aggttatcaa aacagcgttt atgactaaat gaattagagt tatctatcac
     7741 tatttttcaa gcgatttaaa ccaaatggca tacaatatgg catacaaaaa cttcaataag
     7801 ccgtgggggt ttttaaattt cttcttgttc tttaaaatca atgagtggag ctacataatt
     7861 tgaatccact ttttcaaacg tcactttttg catcgacgaa tgttttcttt cccaagataa
     7921 cgtaaataga tttgactctg gacttataaa gtctacttca ccttctagta ttactttctt
     7981 atgcaaatat tcctctaata aatatagact tgctaaagca tacaacacat tttccaaatt
     8041 tgcttcctta taatttgaag aacgaccatg ttttactctg ttatatattt tccaccaatc
     8101 tggtgtatct tcaggccacc catctccaaa aggttttagt gaagtcaatg ttaaagaatt
     8161 cataacccta acaccattat ccaaacacat taattcgcta gatttaataa cagctttaaa
     8221 atcagttatg cttttattgc tactgctatc aaatccgcaa agttctttga aaataatctc
     8281 acactccgaa ccaatcgcta gtagtagttt ttgatattca atagagaatg ttttgaaatt
     8341 attctcatcg agcatcacaa aatctgtggt attaataaac tcattttcta aatgtagata
     8401 atacttccaa taaaaatttg taaactctgc tttgtccatt aaatcacctc ttttgaatta
     8461 taacaaaaaa aacacgtcca caacagacgt gttctttgat ttctacacaa tcagcttgcc
     8521 aaaaccatcc aatcagtcct agcatgcctg tattaaaatt gaacgttcat catctatcat
     8581 ctttcttagt ccagcatgtt tgcaaccaca cctacaattt tcagtccaac aatagatttc
     8641 gactagctaa gtcttgcaaa attcaggcag ctaacccttc atttactacc ttaattatac
     8701 taaataccca caacgtttac aagtgtaatc attctaagaa atctgtttag ctatgatcgt
     8761 tgtataatct ttaaacgccg ttccttcatt gattgaaata attttataac tcttgttttt
     8821 ccaagctact ttcatatcgt ttgttagttc ataatcttga tcgtatcgaa taataaacgt
     8881 caacgttcct tcaagaatgg tacctacact agcaagcacg tcttttagca tttgtgaacg
     8941 cacattagcc caacattcaa atacagtttc ttcactatcc accatctgcc catcttcatc
     9001 ttttacccgt tttgtctttt taaacttgac tctctcagtc aattcattta cattcttaat
     9061 gattggcata ataagcacct ctcatttgtt gtactaatgc ttgtaccgtt actggtatcc
     9121 gttcgctgct ggcttcttga cggttcaaat accaatgttg agtaagtaat gacaccgcca
     9181 aatcaaattg ctgataatta tccatatctt tatccgttgc gctactatca atagcacttt
     9241 ttatatattc gcctgctgta tcaatcaatt gttgaataaa gtcatcatca atagtgtgat
     9301 cgatccgcat actttttttt atgctatcca aaattaccat tttatctctc ctaatgaaaa
     9361 aaaggcagtg ggataaaccg ccgcccaata tttttctata cttttggtgt tacaactggc
     9421 gtaaactcaa tatacactga agcattttga tcaattttct tataatcgtt gcgaacgatc
     9481 actgcaagtc cttgtgagta gtaatcgaat ttttcccatt gagtcgttac ttgatttcta
     9541 cgagcaacaa agatagattg tgcaatatcc cccataatca tagggaatgt tccagcttta
     9601 ggattggcaa acaatgtatc tgcaatcaat actactggca taccgaataa tgatttacca
     9661 ctaggtgctg tcacatcggg ttgtaaaatg taacgtccat ctgaatcttt caatgtatct
     9721 aggtggtaat atccgctttg gtttagaatt accattttat ttaatgctgg gtctaatgcc
     9781 acattgtata gttgtttcaa atcgtccaac gtagcagccg ttttcttagt gaatgttttt
     9841 aataaatcca tgatatgctt attgtcagtg ttttctacca atttcgctaa ctgatctttg
     9901 acctcttgta caatattcac tgctgaatct tccacaacct catttgataa ggcaatctta
     9961 ccagcacgag tttctacttt ataatcaact gaagtaaaca tttcagcgtc aatatcacca
    10021 atttcagcaa gttcagcttt tgttgctaac actgcttgtt ggttagttgc tactggatat
    10081 ttaccttgtc catttgatac tgtttttact gttgcatatt gagctaagtt ataatttgaa
    10141 cgtttcaaat caaagacttc accgattact tcttcgggaa caactgccgc tgcatttaca
    10201 gtagtgactc catcacgcac ttcgccttgt gaacggatat attcttcata cccacgcaat
    10261 tctgtttttt cgttatcgat taaagttttt tccatgttat tactctcctt ttgtgcttta
    10321 gaagtggatt ctaagctatt gtttgtttgt tttgcttggt tgttactcat aaacgattcg
    10381 taagaacgct tatttacttg gacatttgat gaatcataag cgggaacggt cacgacacta
    10441 atttcattaa gtgctttgat tttatctatt gatcgttcaa ttgtgccatc ttcttttttg
    10501 tcgaatgaat ctaatccaag cacgaaccca aaggacatag aatcaacaac cccttttgag
    10561 atattctcat acacatcatt tgaataggtc gtgtcggtta gctctgcttc aaaatgtaat
    10621 cctgtatcat cgacattcaa tttcaacgta cctgctttga cactagctag tggctttgaa
    10681 taatcgtgtc catataataa gaacacattt gataaatcca cctcttttaa tgcttcgggt
    10741 gtaatcactt ctacaaagcc gcctaaatct tttgacggtt gcccgaattt caacgcatag
    10801 cctgaaatag ttttgccgtc tgttggttgt ttttcatttt ctttctctac tttgggagaa
    10861 tcggctgtta gctcagcttc ttctgtcagc cgtttttcct tttcttcttc attattcaac
    10921 tggtgtaact ccttcctgtt cttttaattg atatttctcc aacgtatcta aatacgtaaa
    10981 gttcaaactt gctagtaaac gatcgccacc atccatgggg ggtagcccca ttttcgaccg
    11041 cccctcatta atcgtgagaa gtgacccttt gacctgatcc aatacatttt ttacttttgt
    11101 ttcaggatca gtctctagta actgatcgga attaaagcgg atattggttt caagtttcgt
    11161 atctaattca ctcacaaaca cattaaagta atgaatcagc gtactttgga tatactggag
    11221 attactttga acggtgctag aatgttcatt ttcaacgcct aaacgctcaa tcggtacacc
    11281 aaacgccttg gcaatttgtt ttgtgttcca atcattggaa ttgataagct tcaaaacatc
    11341 ggtgtttatt tctaatgttt tgtagtccat tgtttcatca agaatgatcg ttctaagagc
    11401 attatctcca ctacttgaac cgttggcttc ttcaaacttt tcacgaattg cgttctttgc
    11461 tgatccgtct aaatctgact tatgaacctt caaaatacct gacccactga caccacgagt
    11521 aaagaaattg tgcaatgtgc ggttgccagc ttgttgaatt ttcagttcat cacgtaacgc
    11581 ataaagtggt ggtaatcctg ttaaaccatc ttgggtgaaa tatttgaagt gcaaaatatc
    11641 gctagacttc acacgtcttt ttttatcacc aatctgataa gacaacgtac cattatctaa
    11701 ttgagtaacc gttacacttg agttaggtaa taagtgaatt tcttctacct tttcaccgtt
    11761 gcgtttaatc tctgcaaagc tattaccgtt tagcaacata ttgaccgcca aagcaaactt
    11821 taaatgccaa ccatccattt ctgaattagg tttctcgttt agtaacttca ccagttcatt
    11881 atcagcttgt ggcatattgt ttttgaccaa ttgaatcgga cttgaagcaa tatcactagc
    11941 aataattcga acggctgcaa acacatcact atttcttaat gcacgaacgc ttgtaaagct
    12001 ggtggtgtaa tcatccgatt ggattgatac cacatgttgt aggaaaggat ctcccgttgt
    12061 attttgtctc gctttaaaaa atgccatctt ttaacctccc ttctgttctg tatttagcat
    12121 gaccgctacc ccaataagca ctatgcccag cgctaacaag ccatagtaga catttgttag
    12181 taaggtgata gcaaccaaca cacacaccaa tccaagaagt aaaagaacgg tatgaatgta
    12241 attagaaaga aaattcttca cttgtataaa attcattgtc tgctttcgct ccttccgttg
    12301 tggtgaagta atccattgca aacacatagg cattgattaa tgcagctatt ggatcaattt
    12361 tgttactgtt ctttgattta ttaatctgaa tgccgttgtt atccgttttt aaaatagcgt
    12421 tgttcactgc atgggtgaga atcgtatttt tattgtgaat aacgttgcct tcataaactt
    12481 gttctctaaa agttcgggtc ggaacgttta gagtaatcgt tccttgtctt acttctaaca
    12541 ttgggtagtt ggctttttca gctttactga taagcgaatt agcgttatac gggtcataac
    12601 aaatccccat acattccaaa tcattttctt gaatcaaatc ttgaataaac tgaaatactt
    12661 ggtcatagtc cacgattcca ctttctaatt gagtaatgga acactcacca gctttttcta
    12721 gttctcgata aggcaaaccg tcgcgctttt ctttgtcttg aagcccatat ttggtggcta
    12781 caaagctatg tgaatcacaa taaagttcgc cgttatctag cggtacgatc catgaaacgc
    12841 tagttaaatc atctgtttta gataaatcca ctccgatata aacgggcttg cctgtaatat
    12901 cttgtggttc tacttcggtt gcctgccagt cttcagcaat catatagctg tcctcactcg
    12961 cttgtctcca taaattgaag tttttcacca atacagaatt aaggttattt tgtttcaaag
    13021 caaggttcac atcatcttga atggttggaa tcattacttt tttgatttct tcactttcaa
    13081 aaatcggatt ggctttgatc catttctctt gatcgtgaat ttcttcggga tcgtccaatt
    13141 cccatatcgc tataaagtac ctgtcggctt gttcttcgcc ttttagcaca cgatctaaca
    13201 ataagtattc ctcgtacata ggaacgttta aatcaagccc agcggtacta ataacacaca
    13261 ataagccgtt cttttgttgg gtcattcctg acttaatgac gttgtacgtt ttacgagttt
    13321 ttgcttcgtg ccactcgtca agaattgcgg ttgttggtgc aaacccatct agcgtgctgg
    13381 tttcactagc cagtgccata gcgaacgaat tagagggcaa gtgctgaatc ttagagttca
    13441 taattttcag ttgtggtctt aaaaacttac tagacttgac cacgtttcta agtgaattag
    13501 acagcatatc atagcccaat ttagcttgtt tcaaagcgtt acttacaaac aatacttgcc
    13561 ttgcttctgc tggttcttgt tccataatca acgcattggc agccatgcca cttgcgatat
    13621 acgttttccc attcttacgg ctcatactgg taaacgctcg attgaatcgc ctgtaattcc
    13681 cttctttagt acgccagcca taaagactgc ctagaatgaa tttttgaaat cctaacatct
    13741 tgattgcttt accgtccgta gatggtagca actcaatgaa tttaatcgct ttggtggctt
    13801 gttcttcatc gaacacataa gggaagctat ctgacttaga acgctccaaa tcgcttatat
    13861 gacgctcaca cgcttggata attttcttgg gtgcaatcag cttacctgat aggactttct
    13921 caatgtaatc attcatgaat tcatcaactc tttgaacggg tcttcgggtt catcattttt
    13981 aaccttgttc aaagctagtt ttgatcgtga ttctaaggtc atacctagtg aggtggcttg
    14041 ttttttcaaa tcactcatag cttgtgattg aacccgaacc gctggattgg ctttctttac
    14101 tccgttttcg catgtgataa acgtacctga ttttcttatc tcggcttcgg ctgttttcat
    14161 tcgagcaaag gctaaacaat aggctaccaa tgaaccataa tctgcttcac tcaatggata
    14221 atcttttttt aatgttggta cgatacgatt ccattcactt ctagcgctcg ctgccatcca
    14281 gtcagggggc gtgatattta ttaatggttg ctgcttgaac aattcttctc tagcgtccac
    14341 acggattgct ttttcttcac ttgatatatt gccttttgta tcgtgtaata ttttagggtg
    14401 tcccatgttg tcctcctttc agacaaaaga aaaaggaacg taagcaatta agctcacatt
    14461 ccttcgtttt tcgatcagaa tttttcttta tctaatttat ctatataaat gatttttccg
    14521 ttctgcattt gaatggtcac ttgaccaaaa tgaggtaact caatggtgcg ctgtttccct
    14581 gttttatcag taataacaat tgtatttttc aataaatctc ctactttcat tatatacttt
    14641 ttcacttgtg attacaagtg taatcgttat ttgtaattac ccttatgcct tttcacaacg
    14701 gttatttgta ccaaaatgag taggcatcga ttgtcagtaa gtttcacact atgccccctt
    14761 aactcaaagg gtactctttt ctcgtctttg tctttcattg gctgtcttgg aattatggtg
    14821 tgcatggcat aaagaacgaa aattagtttc atctaaccgt ctattccaat catcttttag
    14881 ttcaattttg tgatctacaa catcagcttt ccttatcaag ccttcttcta ggcaatcctc
    14941 acatactgga tgatttaacc gatacaagta tgaagccttt cgccatgttc tactttgata
    15001 gaattgaaaa tatttacctt cgctttgctt tctttcttca cgcttaattg ccgtttgctt
    15061 tgctttatgc ttcccacaat acttctgcct ataatcaatc aatgttttac atccagcatg
    15121 attacattgt tttttaactg ccatagatta ccaaccaaag cgatctcgtt cgtcagaatc
    15181 atcttgttcc cattttgatt tgtattcaat ataaggcgaa actatcttca agcacgttcc
    15241 gtctttaaaa ttaactttag tgattttatc ttcctcaaaa ctaataaagt ctacatcttc
    15301 ttgagtgttt gaatactcaa tgtgtttctc accatcgaag tatctaatac tttccatatg
    15361 cataattatt ctacctccaa taaattagtt atcaattcat catctgttag tagcaaagcc
    15421 aagaacttat catggatgtt taaaaacaaa ttatcctcat tcgatgtaag ctctcgttgt
    15481 ctcgctcgat catataggtt aagcagacaa ttaaattcat ccattagctc acctgtatca
    15541 tcatagttgc cactatctac caatgatccc actactagcg attgacacca cgctactgac
    15601 tcctgcagcc aatcaggttc ttcttccatt atctttaata cttctggatc aaatgtggaa
    15661 taatcttctg ttaccaatcc cacactaaat gccagcgtgt tcattagaaa ccgatccatt
    15721 tcatctgtat attgaaagtt cggtgctttg tttgttgaaa taatattgag catttacttt
    15781 cctccttgat tcgtaacacg tggtaacacc tacgtaacag gtacccgtta ccactcaatc
    15841 ccaacgggga caagggttct agcgttttgg taacaggtaa cgggtaaaat ccagtaacat
    15901 tattccccag catattagct aactatttca aaagttttaa ggaaataagg tgttaccccg
    15961 ttaccaaatt gctgtatccc ttggggcagt aagggtaaac tagtaacggg ctaggtgtta
    16021 ctgacccgtt actacccgtt actttttgta ctttccagct cgatttttat tttcataggc
    16081 atttgactta ttgtaaaatc ctcgtcgttt atttcttttt ggcagttgct tccgttccca
    16141 accaggaata ctgctaataa tcaaggcaat ctttttagtt tctgcatctg atcgaccatt
    16201 caataaatcc tttgcttgtt tatcaaaaac agcttccaaa atatcagcgg tgagtacgct
    16261 atcaactaaa taaaactctt gttgcatagt agctccatta tagaaatgct gtaaattttt
    16321 ctcattacat ttactgtcag gatagttctg ataataagaa cgtctcgccc acgctggagc
    16381 ttcttcccat ttatacggga tttccatttc caaatattta gtgattgctt ctttaattgg
    16441 attctccatc ttggcatcct cttgatagtc ttttgcaatt tctaagactt ctttatcttc
    16501 atcgggacta aaatagattc gttgtccttt atcaaaaagc acctttgctt cagctaacac
    16561 ttgaagaaaa tattcatcct ttgttttaaa cggattcatc tttggcttat tcttacaagg
    16621 caaaggataa aaacggcggt ttcctgtatc atctttcaaa tactgcccat cattcgacgt
    16681 tccaataaat acacattgcc ttgcccaagc tatgacattc cgaccataag gaggtctaat
    16741 tttatcgatc ttggcactga tgaagttttt tatctgttca attttagttt tgttcattga
    16801 agataattct ccaagttcaa taattacatt gccttgaagc tgcatataat catctttctt
    16861 ttcacctagc gaatctaatg aatcggtgaa aaaatcatcc gtgtaaagta atgctggaac
    16921 ggtactctta ccaatccctt gcggtccatc caaaactgga acgatctcaa actttattcc
    16981 tggtttatag atacgtgcga ctgatccgac tatccaacgt tttgttactt ctcgtgtgta
    17041 aaggttgtcg tctaccccta aaaggtcaat aaacagtgtt tccagtctag gctgttgatc
    17101 ccatttaact ttttcgatac gttcttttac tgggttgaaa acattctttc tagccacatt
    17161 tactactgct ttgtgtattt tgttgtcatc aaaaactaca ttgtagtttt cttcaatata
    17221 tgccgttaag gcatcctcaa aactgtcttc ccattctcca gcggtacgat tgatccaagg
    17281 agaatctttt aataggtgaa tgtatccgct aaattcgtta taagcaattg ttccagctaa
    17341 attgctatca ttggttaaaa tcaattctgc attcacaaca ctgttacttt taatctgatc
    17401 gtctttattg catcttaatc gtgacatcca ggttttcaat tcaatgatat tgtcttcacg
    17461 ctcgctggct tcgggaaagt ctaaaatact atctaattta tgcttatgag ccatgagctt
    17521 tccccctcaa atctttttta atcatcgatc gaataatttg attcacttct ttatctttta
    17581 gcggtggttc attgtacgca tttgctgtat aaatcaattc tgctaaagtt tgattatcta
    17641 caccgtgcca tagtaaccaa ccgcataact tagttaaatg atcgtttcta ctaccttcgc
    17701 cagcgccttg aacaatcata tctaattttt ttgctgtccc tgtttttgct acgggtcggt
    17761 attgagtctt tatttcttgc ggttttttta gcagtaggtt cactagccaa tcgggagctg
    17821 gtgcaatatt atttagagat acgttttcaa taggttcata tgctctccca acacttcttg
    17881 aaataatcat agttggcgct attggaactc ctttagtgat aatatcaatc ccgcttaaac
    17941 ttttaccttt atcatcacgc ccgaagatat cagcaaatgc acctgtttct tgttggataa
    18001 ttacaccatc aggaatttta aagaagaaat ggattccttt acctggtgtg atttccgtat
    18061 aagtagaagg aaatggatca tacattgaag caagtttttt ataattctca acgccgtttc
    18121 gtgtttcaga atgttgatct aggtcaacta ccaccatttg atgatcttca agcattaacc
    18181 caatatttct agttgcatct tccgtccacc attcattaat ttgattagaa tcgttagttg
    18241 catcattcca accactagag ccttttattg gaattttagt tccaggagat aagggataaa
    18301 ttgaccatcc cgacttttgt aattccgttg ctgcttgata attatttgcc aataaacctc
    18361 tcctcctttt tcaaaatact ggtataaatc attcagacac ctccatgagc ttcaactgct
    18421 caagttcgta tttcaaccca tcccgaacaa attcagcacc ttttaattga ttctgatact
    18481 ttacccatgt attaaatgtt ttcacccttg cttcttgcat atcagtaatg tttaaccatg
    18541 cggttaaaat ctgatttttt agctcgttga tttctcgaca aattattttg tgttctgcat
    18601 gttttttgag tacatacaaa actgttagtt tttcattgaa tgaatttcca aagtaggttt
    18661 tcccgatatt attttctagt tcagcgatcc cttgattaat ttcctctaat tcaaggctat
    18721 cgagttggtt taatagtttt gttttgtaca tctacttatg ccctccagtt cttaattgat
    18781 agtcgtactc gtcataagca gtgaagagat ataacgctaa tcctgcaaag atagctattt
    18841 ttgcaattaa tggtaataag ccgaaaattc cgccgattaa aatgcttcct ataattaagt
    18901 ttcgtttcat aatggttccc tccgattttt taatatggta taatcggaac aaagaatatc
    18961 gggttttatc ccaattaagt tgcctttatc ctgtccgcca agactgttat ggataaggct
    19021 ttttctttgt tctcgatcat gttaatgctc ctaagttgtc tgcaattcag cagaacgatt
    19081 ataaattttc tcactcactg aaaactgcaa cccatatttt tctttaacag tcattaattc
    19141 tactgtctca tctaaaatag gttcacgatc aaccaacatt tcaggagtca tatctttctt
    19201 ttttaccatt ttggaatgac cataaatagt tgatacagct ttattggcta tcacattcgc
    19261 tttaatgtag tctactggtt ttggttcagt aatagcgtgg cttaatttag tcattgcttc
    19321 tttttggtgt tctttgtcta gcatgcggaa tacttgaaag ccttcaagtc ctgtggactg
    19381 gcgtagttct ttgatgatgt cgaaaatcca gtcttgaaat gcctcggctt cttttttacg
    19441 gcttttgaat gccatgcggt aaatattttt tttcgttgat gatgtttaca tcttgtgttc
    19501 tgcccaatga atcgatgacc ttactagtag taaccccatc tttaggcaaa gagtgaatgg
    19561 ctctagtaac ctgtttaaga cctaacgctt ccgcaacatc tttcgccact gcccaccatt
    19621 catcattaat atcgacaaat cttataatgt gtccgttcca aatttcagtt ttcattcaac
    19681 gtcctccagt tcaagcccta gcaagctcgc taacaactca acttgctgat acaaaatttc
    19741 ttgatactct tcaatagaaa gtcgttcttt aaactctgct tgtactaaat cgttcaagcc
    19801 acgcaatact ccgcttaccg ttggttcttg tggtagcaat ttttgatagt ccatttgttc
    19861 gtacaaacat tcaaacgttt cttctagcgc tttgccttcc aagtttgcta attctgccgc
    19921 tgtgtcttct gtaagaattg ttgataatac tttttcgatt gctttatttt gtgtcgattc
    19981 cattattatt tcctcctatt aatccctttg cccccacggc ttgcgtttat ttttggtatt
    20041 cttccataaa gcgatcacat tcatcttgtg aaattcttac gactccatca actataatca
    20101 actttaaacc gttaggaaca tatttctgca tcatagtatt gtatgaaacg ttcatatact
    20161 tagcagcttg tgccttgttc atatattttg gaaactggta ctttttttct aataatgaaa
    20221 gtaacgttcc ttgagtttgc tctacttgtt gaacaactcg atcgtcaata attttaaaag
    20281 ctggatcatc aagcgtgagt ttgtcaattt ttaattccat atttatcccc cctcgttagc
    20341 atacatttct atacaaagat tttaaaaaaa tgcaagaggc tcttgaatac atttctatac
    20401 ctcttataaa ctcaatatac catcatacct tttcatttgc aagaatttta acatactttt
    20461 ttgaactttt atgtttcaca catatttaaa tcgtgatata gtagagaaaa tatatgaatg
    20521 gtggtgaaaa tgtggctaga aaaaaatacc ccgaagcagt ggagacagga caaagaatta
    20581 gactggctag aattgaaaag ggactctcaa tggaacaact tggaggtaaa ttatccccac
    20641 cggccagtaa aggtgccgtt agtaattggg aaaatggcta taatcttccc aataacacca
    20701 ggcttaaaca actttcaaag gtattagatg tatccactac ctatctatta aacggatctt
    20761 atacattaag agatatgcat atgatgcccg aaattaagcg aaatgtgctt gaaaagcaat
    20821 tgaaaaaaac tattgaaaaa aataactcta taattgattc taatctttct aatgctatca
    20881 ataacatcga agaagataac ctgactgtct cacagaagat ttatttaaca aacgcaataa
    20941 aatatcttaa aacaggatct cgaattgaat ttcctgacgg gagtacccca attgcttatc
    21001 tcgcagctct tctaaatacg cttttacgat atgactcatt aattaatgat tcagaaaaaa
    21061 atacagatga gaaaaaagag tatcaagaat tttatgaaaa tgatcttcta aaagagttca
    21121 cagctcttgt tactgagcta aataaacact attcaaaata ataataaact gcctcctcca
    21181 accgccccca cggcttgcta cactatggag ggaaaactat tatggcaaca ttcaagcaat
    21241 atgaaactaa aaaaggtaaa ttttggctct atgaagccta tcttggaatg aacaaaatga
    21301 ctggcaaacc tgataaggtt cgtagacgag gttacaaaac aaaaaaagaa gcccagcttg
    21361 ccttgtcacg cttgcaagtg gactacgata aaaacggtat taaaaaagcg aacaacgaaa
    21421 cgtttaaaga agtttatgac ttgtggctag aaacatataa aaccactgtc cgagaagcca
    21481 ccttcatgaa aactgaaatc aagtttagaa agtggattct tccaaaatat ggaaatcttc
    21541 gtgtgaatga agtcacggta aaaaaagccc aacaaattgt aaatagctgg gctgaaacta
    21601 ctgaccaata caaggtactt cattcaactt ctaagcgtat ttttagctac gctatcaatc
    21661 ttgggattat tgagactaat ccactagaca acgtattaat gcctaaaaga accaccacta
    21721 agcataaaga aaaggtcaaa gtttacagta aagaacaact gatcaccttg tttgattacc
    21781 taaacagcaa acctgaaact tatcgaaacg actttgataa aacgttacta cgctttctct
    21841 tttatagcgg ggttaggatc agtgaagccc ttgcattgaa ttggtcagac attgattttc
    21901 aagaaaatac ggtcaccatc aataaaacac taagccaatc caaaaatggc tataaaatca
    21961 gtgatcctaa aactgattgt agtgctgggg ttcttccact agatgaaaga acaatttcta
    22021 tattaaaaaa atggcagatt aatcagcgga aatatatgct gacacttggt gtaactgatc
    22081 cgactatgat tttttgtggt atctataaag aactaatcac acaccacgct atttatgcaa
    22141 gattgattac tatttgtgaa aaagctatgg ttccatttct cggtgtgcat gtagcaagac
    22201 acactcatgc ttcaatgctc ttggacgctg gagctactat ggtagaagtt caaactagat
    22261 tacggcatac aaaaattagt cagacgatcg attgttacgg tcacttggca aaagaaacga
    22321 aagaaaagac tgttgaaaaa ttagtacaac acttaaatat tgactagatg gcatacaata
    22381 tggcatacag taaaaaataa aaaaaggaga aaccctatca tatcaaggtt tctcctagct
    22441 ttatagtcta ttcttcttct tccgccatga atgtattgaa tacttcttca atcatgtccc
    22501 attctgcgtc tgtttcgatt tgttctaact cgccttctgt tccatcttcg ttttcgatgt
    22561 atgcataagc ttgtaattct acatcttcat cttctgaaac accagcagga tataaaagaa
    22621 catagttacg tccaaattct tcttgtccgt caactgttag taggatttca tataatgttt
    22681 catttccttg gtcatccacc aatgtgatgt gttcgtgtcc ttcatggtcg tgggaatggt
    22741 tatgttctgt cataagatta cctcttttct tttattatta aaataattgc ttacctgagc
    22801 cgtctaaata attttgtaag atcatcacag ctgctaattt atcgatgact ttttttcgtt
    22861 tggctcttga agtatttgcc tgctctacta gcatacgttc tgcctgaaca gtggttaatc
    22921 gttcatcttg ataatcaact ggcaaggaga atagttcttt gatcttttct ccgtatgaca
    22981 tggaagcttc tgcccttggt ccgatggaat tgttcatatt tttaggtaat ccaactacaa
    23041 accggtctac ttcatattgt gctactaatt ccttcaaccg atcaaagcca aactcttctt
    23101 ctgcttcatt gatccgaatg atctctactc cttgagctgt ccagccaaag gggtcactga
    23161 tagcaacacc tactgtctta gaaccgacat ccaagcccat tactctcata aattcactcc
    23221 atgatttgag aggtagtcct tgacaattac ttccatgatc tcatcgcgtt cgtgacggcg
    23281 aatcaaattt cgagcatcac gataacgagg aatgtaggcc gggtctccag aaagcaagta
    23341 gccgacaatc tgattgattg gattgtagcc tttttcttcc aaagcccgat aaacaatcgc
    23401 taatgtttcg ctcacttctt ttttgcgact atcgtcaaaa ccaaaacgta cggtttcatc
    23461 tgtaaaaccc atgattctac acctctttcc cgcaaggtaa ttccagctta ccttctacaa
    23521 ccttatttta ctcgaagaaa gggaaaacta caaacaaata ttccaaactt ccgaataaat
    23581 taagaaacac tatttgtatg tttttttcct tttttctgta gctttatcaa aagtactctt
    23641 ttcataaagt atacgctata tttctttgaa tgtatgtttt tacacaattt tttcataata
    23701 ttcataaaaa attcgaatag ctgctactaa ttttttgttt ttgttgcttg tcctgtctca
    23761 taattgatcg tgtagccatc ttgaacattg tagataaaga cattgaattc aatttttttg
    23821 tcttcaatgc tttctgcttc caattgcact cccctggcta ccagttcatt cccctcaaaa
    23881 tgaggagtga cacggtagcg tacatgatga tttgtttctt tgatatatgc cgcaatatca
    23941 ttttcgtgag caagcatgta tggagcattc aacgaacgtg tccctgtcat caagttttta
    24001 atgttgttat tttgaccagt cagctggaaa ccaattaaat ggctgcgatt atataaccat
    24061 tgcccgtcac ttaatttctt ttgtttccaa cctgtcgggt caatatacaa aggctctctc
    24121 ttttcagtag ggaacaaatc ttttccaagc atagcattgg cttgtcctac tctgttcaat
    24181 ttatcaatat ttgaatagct ttcccatgtt cctttggaaa ggctaagatc ctcttttgta
    24241 aattccggct gattcccatt gatttctatt tcttgatgtt ttccatcata ttctggcaca
    24301 tccgctactt gagaagcttg tggtgtggaa tcaaaaatag ttgtaatctg ctgaaagata
    24361 tctgaatctt tttgtgtggt ggaaacaccg aatgcccctg caatcacagc agcgacgatc
    24421 cccacgatcg ccttggttgt ttttttgctc atttctttac tcctttacta acagctccct
    24481 aaatcttaac atatgttccc ctataaataa atactagaaa gaaatagaaa cagcaaaaaa
    24541 taaaacctgt tttaagatgg cttaaaaata caaaaaagag acggattcct cgtctctaat
    24601 cagtcaccac tctatctgta aggtatttat tattattcaa aaattttctt catactcaag
    24661 acaatacact gatttcacaa ccgcttggta aacggtgcgc aaaagcttgc atctgcggta
    24721 ataagtccat ccaagttaaa aacataagaa gttgtttttg cttggctgtt cttattactt
    24781 gttgcagagc agctttttca caacctattt taagcgggtg acgggaatcg aacccgcgac
    24841 atcagcttgg gaagctggga ttttaccact aaactacacc cgcaatacaa aaacagtata
    24901 cgcctctttt attccttcct caagtttttt tattctttac gcttctggta tgttttgtag
    24961 taaaataaaa ctaatcaatc aattattgga gaagatgcta tgacgggaaa agaattagat
    25021 ctgttgctgg acacttgttt actcgctggg aaaatcatga tggaaagcaa tgcggagatg
    25081 tatcgggtag aagatacgat gagtcgaatt gctttagctt ctggaaattt ccgtttggtc
    25141 agctatgtca ctcaaaccgg tctctttatt ggtcttgatc gtacctcgac catccgaatg
    25201 gaacaaatta ccaaccgttc gattaattta gaaaaagtag taaaagtaaa tgatctttca
    25261 cgtaaatatg ttgcttcaga gctaacatta gaagaacttt actgcgaact gcaagcaatc
    25321 gaccgagacc gttctttctt tcctgtctgg ttgcaaatcg ttagcgcggc agtgatcagt
    25381 ggaacaatta tggtgttatt tggcggaaag atgaccgatt tacctgtgac tttagtcatt
    25441 ggaggattgg gatacgcagc ttatctatat agtttgaaat tttttagaat caaattttta
    25501 tcggaatttt tatcttcttt attgattggc atagccgcta tctacagcgt acgttttggc
    25561 ttaggaagca atcaggatct aatcattatc ggatgtgtga tgcctttagt tcctggagtt
    25621 caaattacga acgctatccg tgatttgcta gctggacatt atgtctcagg agtttctcgc
    25681 ggaacagaag cgatgatgac tgctacgatg atcggttttg cgattgcttt tattttccag
    25741 cttttttatt aggaaaggaa cacaagtatg gggacgatat ttattcagtt ttcttttagt
    25801 tttttagcga ctgcggcatt tgccgttatt acaaatgtac ctaggcgttc attgatttgt
    25861 tgtggtcttt ctggtgcttt tggctggatg atctactgga tctgtgttca aataggcgga
    25921 tctgctgctt ttggttcatt gcttggttct ttaggagtag cattcatcag cgatcttttc
    25981 tctaaacgtt tgaaaatgcc cgtaacgatt ttcaatattc ccgggatggt tcctctagtc
    26041 cctggtggtt tagcttatca agcggttcga aatctcgtta ctgaatccta tcgagaagcg
    26101 gctcattatg cggtccaagc aattatgatt gctggagcaa tcgctttagg tcttgtatta
    26161 tcagaagtct ttaaccataa catccgtaat ttcaaagtaa agcgtgacgc aatcatgatg
    26221 aaacgaaaaa aacggctttt taaataaacg aagtgtaaca aagaaaaggt tgtgaaagag
    26281 acgttcagcc ttgagcgatg acaaaatatc tcacaaacat tgcgacaagt aaccgcagga
    26341 gcaataagaa gtaattatga gtattaaaaa aagttttgta aaaatagctt ctcacatttt
    26401 tacataaaat tgacttaata ctgagaaact gacttttgaa caccctctat ctgtaaaaaa
    26461 ggggctgcga catgattttt gtcacggccc ctcttcttta ttcaaatttt ttccaaaacg
    26521 ttcgttgata ataaacgatc catagagcgg aaagtattag aactaaaccg atcacaggaa
    26581 tattcactaa ccaaaatgga taaaacaaag ccaacattcc gtataagacg ataagaataa
    26641 tactgatgat caatgaaccc atcatagttg cgactaaacc caaacttcct gaacctctgt
    26701 taaataattg gttaatattg gtccagttca gcagcagcaa ccgataatct ctagcgaaat
    26761 aacgtagtga caataagtag cagccgaata aagaaccaaa tagcaagcta gggataaaaa
    26821 acaaaggcag ctgaaaagac agtccgctta ataatgcgat cccaccagtt aaaaaactct
    26881 gcaatatcca tccgaatctg aacttttctt ttaaatattg gttcatagag attggcaaag
    26941 aacggataaa cagaaaattt tcttgatcca aagaaataag attgctgata aaagaagtct
    27001 gattaactgt taagaaagcc aatgcgatcc ctaccaggaa aacaacaccg attaacttat
    27061 tatctaaatc agttaatttt atttcgcctc ctagggcaaa agcaagaata aaaatgattg
    27121 gagttaacaa ggaagaagaa agaacttgca taatcaaatt aggttctttt actaattgag
    27181 cattgtaact aaataaaagc tgatgcacat tttggttctt tttatgggtt ctttttattt
    27241 tgaactcact gtttgaatca gtcagttgct ctgtaagctt tggtactatc agctgtttta
    27301 cagcaaggaa aaatcccaga gccgtgatag ctagtattcc ccaacccaat aaaccttgtt
    27361 gagaaagtgg ctgactggca gcatcataga atggcaataa tatcgaaatc ggatgccgat
    27421 ctgttaactc attttcaata gtccccgttt gatgattcat ccacatgatt ccagcaaatg
    27481 cgatcagcat cgagctgcca agcaagatac tagaaactgt ttttctatgt tttttaaaca
    27541 ctgctgttcg agccaaacca aaaacaatca aactagccag acaaaaaaga ataatcaaaa
    27601 atagagaaaa caaaaggatt gagataatga tagtaagcaa aatccctaga tcagctcgcc
    27661 atccggttaa aataaacatg actagaagcg gaaaaacaaa aggaatgatg gtcaaagcaa
    27721 ctaccaaaat ctttgctgaa aaaacctgtt cttgtccaaa aggcagaggc aaaaatactt
    27781 gcaggtcatt tccttcaaaa aagatattat aaatgactga tatactttga gttaaaccta
    27841 acataccaaa aagtgccacg taataagtaa agaaacctgg caatttaaca aagttcatag
    27901 caaacatagc aaatccgtaa atcagtaaaa aaatcagccc tgaaaaaaga ttttgagaca
    27961 aaagatagat agttaatcgc tttccctttt ttcctttctc tcttgcttta ttcgtgagct
    28021 gtggattaac atataaaaga ttaactcgaa ttaattctaa taattgtcgc ttatacatac
    28081 ggtccatctc ctgtcgcttg ccttccagcc attttcaaat aaatcttttc tagcgtttca
    28141 tcaggtgagc cattcaataa ttcactaact gccccctgat aaatcaattc acctttcttc
    28201 aaaatagcca gtttatcgca taactgttgt gcggtatcca atacatgtgt agaaaaaatg
    28261 actgtttttc cttttgcggc atgagccttc atcatttctt tcaaatcaaa tgctgcttga
    28321 ggatcaagtc cttgcaaagg ttcatccaat acccaaatat ctggatctgg cagcaacgtg
    28381 ccgatcaaaa tcgttttttg acgcattcca tgtgaaaaac ttgcgattgt ttcattttga
    28441 tgactataca tatcaaaaag cgctgtcaat tctgctaaac gcttttcttt tttttgtgtg
    28501 tctaattcat aggcagctga aatcaagtcc caatattctc cagccgtcaa ttgtaaaaaa
    28561 atatctgggg aatcaggcac ataaccgatt ttttgtttga tactcaatcg attttccgtc
    28621 aatttcattc catctactaa aatcgttccc gatgatggtt caataatact aactaaactt
    28681 ttaatcgtag ttgattttcc ggcaccatta tgtcctaaaa atccaaaaat ttccccttgt
    28741 cttatcgaga gagacaagtc ttttaatgct tgttttttcc cgtaactttt acttacattg
    28801 atgaattcaa tcatttctgt actccttttg tttgttatta ctttttattg tacccttcgt
    28861 tattggtgga gtaaatgtca aaaagagtgc ataacaaaaa aggatagaaa cgactaggct
    28921 gctacttggg ttgttctctt ctacaggaaa acactcttct aacaggtaag atatgagtct
    28981 ttgttttctt taaaatgatt cattttctat cttttcttgc ataaaattct ttagtttatc
    29041 aatacttctc tttcccatct ctagttgttc tccatttgca aatgttacta tttcgtttac
    29101 tctgctaata gatgtaatgt tttcagtatt aatgatataa gcttttaaat ctttacataa
    29161 atactcatat tcgagtgatt ttttatattt tttaattgtg ccgtttacga gaatctcttc
    29221 atctttcaat actaggccta ttttattttt ataacctggc atgctcatta gataaaggat
    29281 ttcatcgtaa tggatatagt gatcgctatt accataacgg acagttgtcc accttttttt
    29341 cgtagcatgt tgaacaggat agttttcttt ttctttcaat aagtaacttt ggatgctttt
    29401 tttatttttt tcataagtga attcttttat caaataacca ctaggcgtaa tctttcgatt
    29461 aataacagtt attgctttat tcggatgaga cgttagataa acaattttac acttttcatt
    29521 cttttctcta agcatttcgc ctagttcgat accgctataa taggcgttca aatcgatgtc
    29581 taggaaaaaa atatctgagg aatagatttc attattttta atgaattcaa caaattgtat
    29641 aacgttcaat ccctgaacga tctcaaattt ccaaggactt aataaaattc tggattcttc
    29701 caaaatttct actagcattt tttcaaaaac caaactatca tccaaaataa atatacgcgt
    29761 taccataaaa agtacctcca ctcgatattt tactatcaaa atcatttcat acaacttttc
    29821 aacatacatg ttaggaataa attgtaattc tcttcacaat cttttccaac acctttgaaa
    29881 ccgatatcaa atactttcag gatgctgctt ctaaaataaa tatcaaacaa atgaatacat
    29941 catgcaaaaa aagccagaca accgacgtcc ttgcgtgtca gctgtctgag ctatgtttat
    30001 ttattttgtt cttttaaaaa gaacgataca catactgatt tttcggagca gagactttct
    30061 cagcgtttgt aacaactaca gatgggattt cacgcttgaa tggttgataa tagtctgatt
    30121 ggatggttcc ttctatttta taccattcat catttttaac cgttactcct tccggcatat
    30181 gcgtcaacaa accaaagact cctgaatctg ccacacaatg gataatgccg aatcggaaca
    30241 agaagatatc tgtattgcta tcgttcggtg tttggtaaac aaatccttca tagctgattt
    30301 ttttgccaat gaattcgcta gggtaattat aaattagctc cataacttct aaatagtttt
    30361 catctgtgat cttgatagtt tgcttatccc catacttttc tttcaactca ttcatttggt
    30421 ctaagtagtc tgatttgttg aaataaatac tggtatccgg ctttaagtat tgtgtctgca
    30481 tctccggatc ccctactgat tctttactta ctgggaaatt aaatcctttg gcttctacga
    30541 tcgtagtgtc gagactaact gttggaaaca acgtccccac gattactggc aaagctagca
    30601 gtacataagc gattccacgt tgataaggtt ttgacaaccc atggtcatga tcgtgctcgt
    30661 gtgccttttc ttctgctttt ccgtttttga cccacaaata cagctgaact aaagccaaaa
    30721 gagcagataa aatcattgaa agaatagcta agtagcgata atggacattg atatattgat
    30781 cgagtttgcc agaaacttgc aagtacatca tcaattcgaa atacccagat aaaatcaaaa
    30841 accgcagcat attttccctc ctaaataaat aatgtgaatc caataacgct aagggcaatc
    30901 agtccgatca gcgataaaat aaagcgtcct gtaaaatatc gtttcatcat cagtagattt
    30961 ttgatatcga ccattgggcc aaagaccaaa aaggcagcta caggtgctgt gccgaacaga
    31021 cttaacaaag aagatcctat aaacgcatct gcttctgagc aaagagacat cgtaaaggcc
    31081 aataacagca tgatgataat ggctaaaatc tttgttgagc cgagcgttag cataattcta
    31141 gttggtaaat aagtctgcat cgcagctgcg attaatccgc caatgatcaa ataacgacct
    31201 gtatcaaaaa attcgtcgat tgaatgtgtc aacacatgcc cagtttgatg gatcagctga
    31261 ttttttggtt cattcttctc actttgttct tcttcatgga catgatgatg gtgattttct
    31321 gctactttag acgtgatttt ttctttcaat acagcgtctt tttgaaaata ggcaagccag
    31381 acaccaatca caagggcaac aaaaaagctg cctatcaccc gccagcttgc catctgccaa
    31441 gaattgccaa atgcgataaa agtggaaaaa atcacgattg gattgatgat cggagcagtc
    31501 agcataaaag caaacgctgt gtgaactggt acgccttttt taacaaattg atgaacgatc
    31561 ggtacgatgc cgcattcaca tgaaggaaag aaaaatccta agatcgaacc agtaaaaata
    31621 gagaagatac gattttcagg aagccatctc ttcacacgtt ctggggtcaa aaatacttgt
    31681 aaggcgcctg agataagaca gcctaataat acgaaaggta atgcttcaat aacgatcgat
    31741 aagaaaatcg tactcatctg caaaaccgaa tttggaataa aagaaaacat gagtttcctc
    31801 ctttttattt ctagttcatt cgctttgcaa atatacgctt ttattcaaaa aaaacaagtt
    31861 gctctgtaaa tttttacaca actcttattt tttaccaaag cacttcaatt gactttttct
    31921 gttattccca ttaaattaag gtctcataca tatcacttct atcataaaag ggagaataaa
    31981 aatgaaacaa accactaact tgactgaagt tcaagacata ctgcaacagt catccgttgc
    32041 cgtcatttat ctttcgatgc ctaattgcag tgtttgtcac gctgttcgtc cacgactaga
    32101 ggaacttctc acacattatg atattccggc acttcatttg gatgcatttg aaacaccaga
    32161 agtagctagt aaatttgaag tgctgactgc gccagttgtc ctgatttttc atcaaggaaa
    32221 agaagtcttt cggcaagcac ggttcatcga ctttaaaaaa atccgttatc tactagatga
    32281 actgacagca gaagatgatt ccctttctta tgaagaaatc ttccgaacaa aataatcgtt
    32341 gtccaaacag aaaaaagcga ttgaacaagt gaaagttttg ccttcatttg ttcaatcgct
    32401 ttttattttt attctgctgt attttcggaa tgcgcatttg cgtcagaatg caagaaatag
    32461 atcaaggttt gcaattcatt cgtcaaatca acattttgga tcaatacatc tgacggagca
    32521 ttcaatcgag cagcggtaaa gttcaagatt cctttgattc ctgcttccgt taactgattg
    32581 actacttctt gtgctctttc agcgggtatc gttaaaattg ctacttcgat ttgttggatc
    32641 ttgatttgtt ctgacatatc ttccattgga taaacgggga taccgtctac gatgcgccca
    32701 acgatttcct cattcacatc aaaagcacaa ctgatacgta tgctattact ttgatggaat
    32761 ttgtatttta gtaaagcact tcccaagttc cctacaccga tcagcgcaac atttgtcaat
    32821 tgatcgtcgt ttaatgtctt tgcaaaaaag tgcataagat cttctacatc ataaccatac
    32881 ccacgttttc ctaattcgcc aaaataagaa aaatctcgac ggatcgttgc actatctact
    32941 tgaacggctt cacttaattc agttgaagac acttttttct ttccagcatt atttaaaatc
    33001 cttaaatagc gataatacaa aggcagtctt ttagctgtcg cctttggaat aatatgatct
    33061 ttcacgaaat gacctccacg tattgtctaa ttacatcttt gatttctcca tcatagcagt
    33121 atttatagga aaaaatcaat tccattgctc agatattgtg aaattttatt ctttattcat
    33181 ttatttatca tttctgcaaa tctcgcagta taatggaatt tatggtagac tactaattga
    33241 aaaatagttt tttatttgag gagaattcac aaagtagaaa agaggattcc atgatattat
    33301 tacaagccaa tcagattgct cgccattttg gtgcagaagt cttatttgaa aacatacatt
    33361 tagaaatcag tacaggcgct cgtgtcgctt tagttggcag aaatggcgct ggaaaatcga
    33421 ctttattaaa gatcattgct ggaattgaag cacctgatga aggaaagatc gctaaaaata
    33481 aaacagcgac tctcggatac ctcgctcagg acacaggact tgattccaat gaaacagttt
    33541 ggaacgagat gctgaaagct tttgaacaag ttagacaaat ggaagagcgg atgcgcgagc
    33601 tagagatttt cattagcgaa ggtacgccag acacacctgc ttatagttct tatttaaaag
    33661 aatacgaccg gctgcaacac gatttttcag atcgaaacgg ctatggttat gaaaatgaaa
    33721 ttcgttccgt attgcatggg tttaaattcg attcttcatt ttatgatcag aaaatcgata
    33781 ctttatcagg cggacagcgc acacgtttag ctttagctcg catgcttttg caacaccctg
    33841 acattttgat tctagatgaa ccaacgaacc atttagatat cgagacactt tcctggcttg
    33901 agaattatct gcaaagctat actggtgcat tattgattgt ttcccatgac cgttattttt
    33961 tagataaagt agtcaatgaa gtctatgaat taagcagaag aaaaatgcgc cattacaaag
    34021 gtaactattc gaaatatcta gatttgaaag cagaacagtt agcaagtgaa tggaaggctt
    34081 atgaaaagca gcaaacagaa atccataaat tagaagactt cgttgctcgg aaccttgttc
    34141 gcgcctctac cacaaaacga gcgcaaagcc gcagaaagac attagaaaaa atggaccggc
    34201 tggatcgtcc tcaaggggat gaaaaacaag caaactttct attcagtata gaaaagcctt
    34261 ctggaaatgt agtcttgcaa atcgaagatg ccgctatcgg atatgataat gtcaccttat
    34321 ctgaaccaat caatctagat atccgtcgtc aagaagcgat tgctctagta ggtccgaatg
    34381 gtatcgggaa atcgacgttg ctgaaatcaa tcattcacca gatccctttt atccgtggaa
    34441 aagaaacgct agggacaaat gtcaccatcg gctattacga tcaaggtcaa gctgatctgc
    34501 atacaaacaa gacgatccta gcagaattgt gggatgagca tcctaccact ccagaaaaag
    34561 agatccgaag tgctttaggt gctttcttat tttctggcga agatgtagaa aaaccaatcc
    34621 ctctgctaag tggcggtgaa aaagcacggg ttgctttagc caagttagcg atgaacaaag
    34681 aaaatttttt gattttggat gaaccaacaa accatttaga tattgacaac aaagaagtat
    34741 tagaaaatgc attgatcgat tatgacggca cactgctttt tgtttcccat gaccgatatt
    34801 tcatcaatcg gatcgcgaca aaagtcgtcg aactctctga aaatggcagc aaagtctatc
    34861 taggagacta tgattattat ttagagaaaa agcaggaaga agaagaaatc gccgaattgt
    34921 tggcacaaga tgagccagaa tctgctcctg ctcccacgaa caaatctgat tattaccaaa
    34981 gcaaagaaca gcaaaagctt atccgtagcc ttcaacgaaa aattactcag gttgaagaag
    35041 aaatggcgcg gatcgatgaa tggatcgatc agttggaaca agaaatggtc gcgccagaaa
    35101 acctggaaga tcacgtaaaa ctcaatgagc tgaaccaaga tttagaagct gctagacaag
    35161 atcaagaaac aaaattgacg gaatgggaag aactaagctt ggaattagaa gaatatgaaa
    35221 attaacgaaa aaaccaaaac agaaaccatt catgctgttt tggtttttct ttttttattt
    35281 tatcaaacaa aaattccata tttacctttt tcaccaataa gaagaccaat tactagtcat
    35341 tctcactaaa gcttctaaat aatagtaatc accccaaata caaggtgtat ttaccccatt
    35401 atttttgggc ttattataaa cagactgtgt taaaaagcca tcttcttctt ccgttgtagc
    35461 atatctttct ttcaattgtc gcataatttt ccaaacagca gactggtata agttcttttc
    35521 tgttttgtct aatagacctg attctattaa ttctaataat ccacaaacca tgatcaccat
    35581 actagatgta tctttttctt ctctgctaga atcatcaaag atcaaatccc aataacaaat
    35641 ggtatcttct ggcaaccgat tcataaaata attggctaat attttgctta aatcaaaata
    35701 tttttgttct ttcaaataac gataacttaa tgcaaaaccg taaatccccc atgcttggcc
    35761 tctagcccaa caggaattat cactataacc ttgatgcgta cttccatatc ttggttttcc
    35821 atctttgaca tccatataaa atgtatgata agttgaacca tcttctctta ctaaataatc
    35881 tgctgcattt tgaatatgag cctgagcagc ttttttatat ttttgatttc cagtttcttc
    35941 actcgcccaa tacaataatg gaagattcat gcaacaatcc atgatcattc taccttgttg
    36001 tgaaggatca ctaggatctc cccaagcttg tataactcct gccttctccc aaaaacgctg
    36061 aagtaatgtt tctgcagcat caattgaagc ttccttcatt tttcgatttc ccgttatttt
    36121 ataatctgct acggttgata aagaatacaa aaaaccaaga tcgtgatgat tagtgataat
    36181 ttgacagtca atcctgtgga taaaatctat atctgctttt tcagctgttt gttgaaataa
    36241 aggatcaccc gtcttctcaa ataacaacca attaattcct gtccaaaatc cttctgtcca
    36301 accaatattg tctcttaatg gataacgtcc ttttatagga cactcgcttg ggaattgata
    36361 ctgactgtta ctgatacgtt tgattaacct ctccgtgtgt ctgcgacaac gctcgatctc
    36421 attattccat tctttttcca ttaattcttt ataacattca tttatccatt tttctggatg
    36481 tattatctct tcaaacatca ctttatcaag catttcccca ccagccttta gcataatttt
    36541 agtctttttt tattgttttt attttggata gttagcccgg ttgtcccatc aaaatcaatt
    36601 ttgggacgat tgttccatac ttctaaacta taatcccgat cttgatgagc aaccactcct
    36661 gttccgagcc aatgggttcc ttttccaaat gtaccctcta atccaggaat aaaagtgttt
    36721 tcccatgtca tcaagtttgt attgggcaaa cccataatag ctgtggattt tcgtttaata
    36781 gtatcgtcac ttaaacagat aatacctgaa aagccagctt cactcaccct atagttttcc
    36841 ttgtcttctt ctatgattat tccttctttc tcaggagaac attcaatagc aaaccctgtc
    36901 tcatatagaa catattctgt atctaattct actttgtgta cgcgaatctg ccagccttca
    36961 aaaggaataa gccaagttct gatttttgcc tgtaaagtag gttcccatgt agaggcaagt
    37021 acctctttct tacatacaat gtctcgtaca ttatttcgaa cgatgaactg tccataggtt
    37081 ttgatgctct cattcttaaa actggtcgtg atatatgtgt ccgctcgctg aatccctagc
    37141 attgaatcca ttgccatttc tgctttcaat tgattcgctc gcggtatcga aaaaccaaat
    37201 tgactagaat aagcaaattt taaatatttt tcattggtca atgtatggaa atttggccct
    37261 aattgtcccg cattcaaaaa aaatgtatgt ccattatcat gttgtatgag caaattggct
    37321 tctgaaagaa gtttagttcc ttcacgaata ggcataggca tttctttagc cgtccaatac
    37381 tcatggtctt caggtagcgc caacaataaa aaaatcttat tcagccataa tggagataac
    37441 gtactattat agggttcagt caatgtcaat tgagggtagg tataaccaat attcaggata
    37501 cctttttcat caaatatttc tttttgcatc caccatctta tattccgatt gactaacccc
    37561 ttgatttcac ctaatttaaa aggcaaaaga tcggcccaca acatagctga ccaaaagctt
    37621 gaaactgcaa aacgataaat catgcttcga ccaaaaggca catttgcacc atcttccgca
    37681 aaaaaatgaa tatagtctct agaaaaatca atagctcggt tcttatatat ctggctaatg
    37741 ggcttttctg gttctagtac gctataaatc aaaccataat aatgaaatgc aaaaggatta
    37801 taataatctt ggcgacctct tgaactgtct cgataccagc cattttgaag ataacaattt
    37861 tctatattat caaatgcttc tttcagtgct gattctttaa ctggataacc aaatttttgg
    37921 ataattgttc cgacaattat cttaaaaaat tgccaattac catctgcaca ttcatattca
    37981 aaaatttgcg agaaccaatg aataatctgg cttttttctc ctgtatctaa ttttttccat
    38041 aataattgtt catgatgata taacgctaat aacattgcgg gcatttctac catacgctgg
    38101 tctctttcat gtatttctcc ccaataatcg ggatgggaag gatctgttcc agcttttatt
    38161 cctgatagaa ttaatttcaa ccatcgttca tctaattggt caattgcggg ccccgctccc
    38221 cataaaaccc gagagaatcc ttccattcct gcaattttat ttccatatcc taccccttga
    38281 tgtatataat caatttgtgc gccacttgct cgataaaacg gctggagtgg ttgatttatc
    38341 tttttaaata agtcaaagta atcctgtctg gtccttaata gtggtactgg atgtttcatt
    38401 gtattttctc ctctttttac taccaaatag ctaattccga ctgttctaat cgaagtaagc
    38461 cttcaataaa aaaataatca gaatagatca gtggcacatt ataagtcagt tctctatggt
    38521 atgcttctgc tccgcctcca atgatcccat cttcatccat attccaattt gcatagtgct
    38581 tttctgtctt ttggagtata ttgatagcag cttcatgata catttttgat cgtacagagg
    38641 aaacatatcc tgctaattcc agaagaccgc atgcagcgca tactccagca ctggtatcaa
    38701 tattcgaatc ttttacagca aaatcaatga gaggaatata accagttttt tcgattttct
    38761 gaataaaatt tactgctatc ttttctgcta tagctagata gtacggattt tttgtgtaac
    38821 gataacttaa cgcgaatcca tataaagccc atgcatttcc tctgctccaa caagaattct
    38881 cattgtatcc ttgcccgcca agtaaccgaa tatatttccc tgattctgca tcaaattctg
    38941 cgatatgccc ggttgatcca tctttcctca ctagatactt agctgttgta tctgcatgct
    39001 gccgagcaat tttagagaac ctcggatcac cagtttccct cgaagcccaa tacagtaatg
    39061 gtaaattcat catactatca ataatcatcc aaccaggttt atggtcattc catgctacta
    39121 aatagttccc tgaaatatta tagcgtccag caagtaccgt agctgcatgt aaccctagtt
    39181 gtcgactatg tttgttttga gactgttgat aatgtgcaat agccgatggt aagaataaaa
    39241 aacctacatc atgatgaacg tctgaaaaat ggtacagggc agattctaat aattgttctt
    39301 gttgtattgc tgcctctttg aacttttctc gttttgtctt gtgaaagagc tgccataaaa
    39361 taccactcca aaaaccattg gtccaccagt cgattcctct gtcctttatt acgtctgtat
    39421 attttccgtt gtaagaaata tatggaattt tattccccat tctgtctaat tccgcatcta
    39481 tcttttcaga agctacattc aaaatttttc ttgaccattc cttcgtctgt tcattcatgg
    39541 atatcccgcc attcttttaa tttttatgaa tgtggagttg ctcctagaac gaactagatg
    39601 gttaacattc gttatttttt ttacttattt gcagctccaa tgtcattctc cttaaattta
    39661 gactgcgccc acacataaat caagaccatt gggatcactg aagttgctaa aattagcata
    39721 attgcgccat aattaattgg ctcacctgta ctagaaaccg tcatattttt gataattaag
    39781 ggaaccgtat atttatcttt atctgaaagc atgactaatg gaaatagata ttgattccaa
    39841 cttcccataa agataagtaa accactagta ataatcgtag gtgataaaat cggtacagcg
    39901 attttcgtaa aaatcaccca ctcattggca ccatcgattc tggcagcctc catcagtgct
    39961 gtaggaaatt gactgctata ttgtcgcatc ataaacactg aagttgtact ggcaatcgaa
    40021 gggatgatca cacctgcata agtattcgat aatcctaaac gactcataat gtaaaataaa
    40081 ggaatcaatg tggtaaaaaa aggcaccatc ctagatagca taactgtatt gaacaacagt
    40141 ttattcccca taaaacgata ctttgctaat gcataacccg ccattgtaga actggctgta
    40201 gctaaaactg tactgattac agcaataaca atcgaattcc aaataatcaa gggataatta
    40261 tatctcgcca tgatggcttg ccagtttttt aatagcatat ccccaaactt gaaattgatt
    40321 ccgccactta aaatttcttg attactttgc gttgaagcta caatcatata taaatatggc
    40381 atcaaaaata ttaaggcacc aaacacaata atgacgtacg taaaaattct gctttgtatc
    40441 ttttgtttag tcatttttat cacctccgaa tttaaattga atagccgata atataacaga
    40501 tacaaacacc atcgtccagg caatagcaga ggcttttcca aaatcaacca tttcaaaact
    40561 tgttttatat aaattgactc ctacgaccaa agctgaattt tctggtccat tggaagtccc
    40621 aaagaatata tttgggatat tgaaattacc caagccattg atagtggcta aaactgtggc
    40681 cattaaaatg attggcttta gcatggggat cgttaataaa aagaattgcc gaaatttcga
    40741 tgctccatct atttcagcac tttcgtatat ctctttagaa atcccttgaa gaccagctaa
    40801 aaataaaata gtgttatatc ctacactaag ccaaatcatc gtgatgatca ttgatattcg
    40861 tatccaaaaa ggatttgtta accactgtac accgttaatt cctagcgcat tcaaaaatcc
    40921 attgaataac ccagtcggat tgaaaaaagt catgaatacg gaagctacag ccaccgttga
    40981 tgtaatggtt ggaatataat agacagttct aaaaaatcct tttcctttta actgtgctga
    41041 attcaataaa acagcaaagt aaatagctaa tagcatcgtt attggaattg atcctgccat
    41101 gataatcaaa acattcgtgt aagatttaat aaacaatgga tctcggaaga tagctagata
    41161 attgtcaaat ccaacaaatt caaatgagta gccaattttc ataaaactaa aagcaaacga
    41221 gataatgatg ggaatcaaaa tcgtcacaat aaagaaaata acaaaaggtg tgaccaatcc
    41281 ataagcactt tttttatgag acgtatctgt tgcttttttc ttgctgttct ttattgtctt
    41341 gcttggtaac gaagattgca tcatcgttaa gtcctcctaa tattttttaa tcaccagtta
    41401 attttacgcc gtacttatga gcaaaatttt ctgcttcttt tagatatgaa ttttcaaaat
    41461 cacctgtttg ccaatagtta tatgcagcgg tagtcaaata agacgtagca tctgaataag
    41521 tggctggtaa aacagtattt gatgtatttt cattgatttt attcaatagt tcatagtatt
    41581 tttgtcctcc ttagtagtca aactttttct cagctgcttc tgtttgatag gccaatgagt
    41641 tagatgtagc cacgcccagc tccagtgcgt tggctaagca ttcttcatct gtcagcataa
    41701 atgtaagaaa ttgttgtgcc acttgtggat ttttggcatc tttagggaca taaaaacttg
    41761 atcctcctga tactgcacct tttcctggat tttcttttga aaaaggaatc agtggagcca
    41821 tgcgccattt gtcagatgca tcaggaaaat tcaaagacat attcgtagca taccagccgc
    41881 cagcaacaaa cattgcagat tcttgaaatg ctttctcagc atcttgtgca ccgtagaaat
    41941 gtacaatatc tgagtcgatc atttctttga ttaactttgc agcatgtttc gcttctttag
    42001 tattcagacg gatatttcct tcttcatcaa gcaaagattg attctgctgg gctagcatgt
    42061 tgataaacaa ttcttggtcc ccagattctg gcaaggcaat tccaaaacta ccagtttttt
    42121 cttttaatgc ttttgccacc tggatatatt cttcccaaga actgatttct tcaaaatcta
    42181 cacctatttc agcaaatttt tttggttgat aataacaaag tgctggggta aaatcactag
    42241 cccaaggaat aatcgtttca ttactcgttt gttgacgcaa aatattcaat ttttgatcat
    42301 aaaactgatc gccatatttg tcataaaatc catattcatc agcactataa aaagcatctt
    42361 gaaatttttc cagcacacct gctgctgaag tgtctgccat ggagataata tccggcatat
    42421 cttcgtgtgt taccaatttt ggggtgatca tttgtactgt tgctgttccc gaagctacat
    42481 tcacaatttt taagtcaata tttgctccag ttttgcggtt atagattttt gcagcatttt
    42541 caacatagtt tgtcagtttg ccgtttgcga catatacttc taatgtagtg gcttttttta
    42601 tcattggatc tgcgatgttt gctgttactt cttcgttagt ttttccacat gcacttaagc
    42661 tgaataatag ggtaatgttt agcaaagcta tcaccaattt gtatcttttt ttcatgtcta
    42721 tcccttcttt cttttcaagt tcttatataa aacgctttca cttatgtata ttaacggaat
    42781 tagtaacaca aatatttata taaaaaaata ttttacttgt cgttttggct tttcattttt
    42841 gttataatag tcattaatta taaatagaaa gctttttata taaaaagaga aaggaaagtg
    42901 gttcttttgt taacaaacaa ccaatcctcc aatcttgtca aaaaaacact aacctattcc
    42961 atagataccc atcttcattt atctgctgct ccctattttc gactactaat aaatgaaagc
    43021 gctaatttat atgctgaatt tgcacataaa tctatagaac tagataatcc ttccctttta
    43081 ttcattgata aacagcaaga agtccaaatc ttttctactc aaaaggaaac acaaactttt
    43141 atcattatcc agttttattt tgatgagcaa ttattagcac atattacggg acaatctttt
    43201 tctatagatc aagaaataac atctatcatt acttttctga aaaatgaaca gttcaaaatt
    43261 gattatctaa aacatagtaa gccagctatt tcctatgatc aggaaatcaa ttcttttttc
    43321 atactatgcc aacagatgag cattcatttg ctttccttat tagttactta ttgtctgcaa
    43381 aaaattcaat tgtcttcctt accaaggcat tatcaacagg aacttgcttt attgacaata
    43441 ccttcaaatg agaaagtcat tcctaaaaaa aagcatattc tttattcaga tgaattagtt
    43501 gatccaatca tacgatattt agaagaacat ctttcagaaa attttttgct tgaacaatta
    43561 gcccaaattt tttttatcag tccagccagc ttaaaaaaac attttagaaa agtgaatgga
    43621 aaaagtgtta ttagctattt taaagaacta aaaatggaaa aagcaaaacg tctcgtgcta
    43681 gaaaatgaat taacctatac agaaattgca gatatattag gatttcattc gatccatcat
    43741 ttttcttcag cttttaaaaa atatacaggg ttatcccctt caaagtatta tctatcctta
    43801 aaatagaaaa aaatttttag gttttatcca caaattattt ctctatattt tctgtctatt
    43861 caaaataaac aataattttc cggcacaatt atttgctgtt ttttaccata acatagtacg
    43921 atcaacgtag aaaaggagat gtttttatga aagttgttgt cattggcggt gtagctggcg
    43981 gtccttcatt tgccactcgt ttccgtcgat tgaatgaagc gcacgaaatc attatctatg
    44041 aacgcggaga aaatatttct tatgcaagtt gtgctttgcc ttattattta ggaggtgtga
    44101 tcactgaccg tgactcgctg atcgaacgta caccagaaat attgaaaaca aaaaacaaca
    44161 tcgatgtctt tactaaacac gaagtaacag caatcgatcc ttctactaag cgattaacag
    44221 ttaaagacct atccacaaat gaagaaataa aaacagatta cgataagttg atcatctctt
    44281 ctggtgctag accagattat ccggatattc ccggagtttt tgaagcagaa aacggctttg
    44341 tcctccgtag tgtgacggat gcggatcgaa tcaaatcgtt ccttgaagaa aaaaatccac
    44401 aacatgtcgt cattcttggt gcaggtgtta tgggtctgga attagctgag aatttcaagc
    44461 atcgcggctt agacgtgact ttaatcgatc aattgccaca agtcgctttc ccttatgatc
    44521 cagaaattgc taatttagtt tatgacaaat tgctgaaaga aggattagcc gttcatttag
    44581 aaacaagagt tactgagatc cgtgataaag gtcgagaaat agtattatca gatggctccg
    44641 tcctttctgc tgatatgcta atttttgctg ttggtgtttc tccgaagaat gaagtggtga
    44701 aagcaaccgg catacaatta tcagatacag gtcagatcat tgttgatgac cagttacaaa
    44761 ccaatctgcc ggacatctat gcgattggcg atattatcga aacgactagt gtagtgactg
    44821 gtcagccgat ccaaagtatg ctttccagtg cggccaatcg tcaaggacat atgctggcag
    44881 atattttaaa tggtacgcct atgcgctatc gcggatatat tggtgcaggt gtcgcaaaaa
    44941 tctttgatca tacagcaagt tatgcgggaa tgacagaaca tgaattaaaa gcatcaggca
    45001 taacaaatta taaaactgtt tttatcactc cctttgacca tgcctatttc tatccagaag
    45061 ctacaagatt aaatctaaag ctgatttttg atgcagatag cggtcgtatt ttaggtggac
    45121 aagcatttgg agaaaaaggt gtcgataaac ggatgggaga actttctgta gcaatcaccg
    45181 gaaacttgac agtctttgat ttgcctgatt tggagttgcc ttactctcca ccgtattcta
    45241 ctacccgtga cccgttgaat atagctggtt atgtcgcaat caatcaaatg acgaatatcg
    45301 tagaaacgat caaagcaagt gatatacccg aaaacgattt gaaagaagcg ttctttttag
    45361 atatacgtga acctaataaa gcaccttctg gcagtatctc ggcaacaaaa aatattccaa
    45421 tgaatgagtt acgagatcga atcaatgaaa tcccaaaaga taaaaaagtt tacattactt
    45481 tcagaagagg attgaatact tatacttctg cccgaatctt ggcaggtttg ggtatcaaag
    45541 cgattttgat tgaagaataa aaaaacgacc gcggtgggta gccgcggtct aggagttatt
    45601 tttacttgag gagtaaaaat gaaaaagtat atagggttgt tggttggtat gtatatagaa
    45661 tagaagataa atgtgaagaa attgtgcatt atttcttcac atttattagg ttatagttta
    45721 agaaagttta aacattgcgc aatttaatta ttttagctgt ttcttttcat aaaaaaacat
    45781 gttgtgactc tgagacaaat catagtccat agtgatgtct ctagagtaac tacgcctaat
    45841 attacgaggc atcatattaa tctatcagca ctagtaccta aactagcatc cctttcgaat
    45901 attttttctt atataataaa gtttgctttc actcctctaa tctcaccaaa tcgactggct
    45961 tgcgttgtgt gatactagga actgattggt ttagaacgac ttttctattg taatcatatt
    46021 tttcgccatc ctcgttgtct tctaatcctt caatgaatga tccctaatgt gtgtattaaa
    46081 taatttaatt tttttgttcc tatttattta a
//
LOCUS       DARBDV010000010        44939 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000010 DARBDV010000000
VERSION     DARBDV010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.010"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(38..1189)
                     /locus_tag="SCM56_000581"
     CDS             complement(38..1189)
                     /locus_tag="SCM56_000581"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEG1109405.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="SCM56_000582"
     CDS             complement(1202..1639)
                     /locus_tag="SCM56_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1109406.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="SCM56_000583"
     CDS             complement(1654..3420)
                     /locus_tag="SCM56_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HEG1109407.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="SCM56_000584"
     CDS             3684..4361
                     /locus_tag="SCM56_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1109408.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4364..5491
                     /locus_tag="SCM56_000585"
     CDS             4364..5491
                     /locus_tag="SCM56_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1109409.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5691..6131)
                     /locus_tag="SCM56_000586"
     CDS             complement(5691..6131)
                     /locus_tag="SCM56_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1109410.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6142..8175)
                     /locus_tag="SCM56_000587"
     CDS             complement(6142..8175)
                     /locus_tag="SCM56_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEG1109411.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8177..9880)
                     /locus_tag="SCM56_000588"
     CDS             complement(8177..9880)
                     /locus_tag="SCM56_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1109412.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9974..10924)
                     /gene="manA"
                     /locus_tag="SCM56_000589"
     CDS             complement(9974..10924)
                     /gene="manA"
                     /locus_tag="SCM56_000589"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEG1109413.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10963..11988)
                     /locus_tag="SCM56_000590"
     CDS             complement(10963..11988)
                     /locus_tag="SCM56_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEG1109414.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12197..13066)
                     /locus_tag="SCM56_000591"
     CDS             complement(12197..13066)
                     /locus_tag="SCM56_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HEG1109415.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13525..15417)
                     /locus_tag="SCM56_000592"
     CDS             complement(13525..15417)
                     /locus_tag="SCM56_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1109416.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15414..17141)
                     /locus_tag="SCM56_000593"
     CDS             complement(15414..17141)
                     /locus_tag="SCM56_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1109417.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17157..17807)
                     /locus_tag="SCM56_000594"
     CDS             complement(17157..17807)
                     /locus_tag="SCM56_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEG1109418.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18011..18307
                     /locus_tag="SCM56_000595"
     CDS             18011..18307
                     /locus_tag="SCM56_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEG1109419.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18479..19099
                     /locus_tag="SCM56_000596"
     CDS             18479..19099
                     /locus_tag="SCM56_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HEG1109420.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19191..20357)
                     /locus_tag="SCM56_000597"
     CDS             complement(19191..20357)
                     /locus_tag="SCM56_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEG1109421.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20369..20722)
                     /locus_tag="SCM56_000598"
     CDS             complement(20369..20722)
                     /locus_tag="SCM56_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HEG1109422.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20850..21872
                     /locus_tag="SCM56_000599"
     CDS             20850..21872
                     /locus_tag="SCM56_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEG1109423.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            21891..22052
                     /locus_tag="SCM56_000600"
     CDS             21891..22052
                     /locus_tag="SCM56_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109424.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(22311..22745)
                     /locus_tag="SCM56_000601"
     CDS             complement(22311..22745)
                     /locus_tag="SCM56_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109425.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22855..24714)
                     /locus_tag="SCM56_000602"
     CDS             complement(22855..24714)
                     /locus_tag="SCM56_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEG1109426.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(24832..25992)
                     /locus_tag="SCM56_000603"
     CDS             complement(24832..25992)
                     /locus_tag="SCM56_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HEG1109427.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26299..27723)
                     /gene="araA"
                     /locus_tag="SCM56_000604"
     CDS             complement(26299..27723)
                     /gene="araA"
                     /locus_tag="SCM56_000604"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HEG1109428.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27739..28437)
                     /locus_tag="SCM56_000605"
     CDS             complement(27739..28437)
                     /locus_tag="SCM56_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEG1109429.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28394..30055)
                     /locus_tag="SCM56_000606"
     CDS             complement(28394..30055)
                     /locus_tag="SCM56_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEG1109430.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30273..31673
                     /locus_tag="SCM56_000607"
     CDS             30273..31673
                     /locus_tag="SCM56_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEG1109431.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31925..33004)
                     /locus_tag="SCM56_000608"
     CDS             complement(31925..33004)
                     /locus_tag="SCM56_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1109432.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33090..34049)
                     /locus_tag="SCM56_000609"
     CDS             complement(33090..34049)
                     /locus_tag="SCM56_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEG1109433.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34061..35572)
                     /locus_tag="SCM56_000610"
     CDS             complement(34061..35572)
                     /locus_tag="SCM56_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEG1109434.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35594..36157)
                     /locus_tag="SCM56_000611"
     CDS             complement(35594..36157)
                     /locus_tag="SCM56_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEG1109435.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36163..37026)
                     /locus_tag="SCM56_000612"
     CDS             complement(36163..37026)
                     /locus_tag="SCM56_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1109436.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37040..37936)
                     /locus_tag="SCM56_000613"
     CDS             complement(37040..37936)
                     /locus_tag="SCM56_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1109437.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38111..39400)
                     /locus_tag="SCM56_000614"
     CDS             complement(38111..39400)
                     /locus_tag="SCM56_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1109438.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39372..40889)
                     /locus_tag="SCM56_000615"
     CDS             complement(39372..40889)
                     /locus_tag="SCM56_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEG1109439.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            41087..41977
                     /locus_tag="SCM56_000616"
     CDS             41087..41977
                     /locus_tag="SCM56_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEG1109440.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="SCM56_000617"
     CDS             complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="SCM56_000617"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEG1109441.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(42886..44358)
                     /locus_tag="SCM56_000618"
     CDS             complement(42886..44358)
                     /locus_tag="SCM56_000618"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEG1109442.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
BASE COUNT        12989 a         9387 c         7783 g        14780 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgca ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact
     4501 gaatcataac tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga
     4561 gaaagaattt gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc
     4621 aaaaaacaca gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat
     4681 actggatcga ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg
     4741 tgtcgagttg ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt
     4801 gccagcgcat ctccctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag
     4861 ttttttgatt ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag
     4921 aaagcaggta atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat
     4981 ccgttttacc atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt
     5041 ctctatacta gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat
     5101 cattggtcag gagatacgaa tccttccgat gtatgagtat cttttccaga gctttctgtt
     5161 cctgttgttg tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga
     5221 acattctcta gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt
     5281 gttttcttcg attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga
     5341 ttttcaaaat gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac
     5401 tttcatgaat ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat
     5461 gggcactatc tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa
     5521 aaccttgtca ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa
     5581 gcttgcatcc gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg
     5641 gttgaagtta ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt
     5701 ccatgttcaa aaatcatttg gacttcttca atggttttcg catcactgag cgccattact
     5761 tggtcgatgt ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt
     5821 tttaaagcca ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc
     5881 acaccatcag gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca
     5941 gtcccatgtg gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg
     6001 atcattgatt ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc
     6061 tcggtcaacg cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt
     6121 agctgtatca tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa
     6181 ctgataaacg atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat
     6241 gattgctcca ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc
     6301 cagcataact aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa
     6361 cggatgtttc aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg
     6421 gaataacccc atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag
     6481 ccggcgatga accacctctt catccgttac tagtccttca ggaaaataag gaaataagct
     6541 gcgaatcgtt tcggaaatcg tctcaggatt atccagttct tggataaaaa aacgaccgag
     6601 taattgattg atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca
     6661 ctcgttattg ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc
     6721 ttcttttagc tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc
     6781 accgtatcct ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat
     6841 tttttttaaa tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt
     6901 caatcgtagt tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact
     6961 gatattgctt tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc
     7021 gatctcttct tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc
     7081 attcgcaact tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc
     7141 ttcttgtaaa acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa
     7201 agtttcatct tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt
     7261 tactaaataa gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt
     7321 cgtgaagtca tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt
     7381 tgtctcagaa gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc
     7441 cagactatta tatgtctcat ttgtcagtaa atgattttta gtcacacgct ccatcgtcaa
     7501 tgtcagcatc aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat
     7561 caattcttcc ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa
     7621 aaaataattc agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc
     7681 taattctaat acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta
     7741 tccctggttc cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat
     7801 atctgcataa aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa
     7861 cggctctgtc gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa
     7921 gtcattactt ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc
     7981 aatgacaaat gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac
     8041 ttcaagatta tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat
     8101 tttttggata gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct
     8161 gggcgataaa tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta
     8221 cccaatgttc aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta
     8281 gttctctttg tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt
     8341 ttgagataac aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca
     8401 tagtttgtga gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgactttt
     8461 tttgttctgt gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt
     8521 cttttattcg tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac
     8581 tttgaggtac tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat
     8641 ttacttggtt attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg
     8701 ctgctaattt ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc
     8761 aagcaaaaat gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg
     8821 ttttgttctc tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca
     8881 caaatgtact gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg
     8941 caaaaatcac gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt
     9001 gaaatatcaa cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca
     9061 gcaagacaaa tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc
     9121 cactcgcatt tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta
     9181 aaccaggtcc aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga
     9241 attcgattcc taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt
     9301 ttagaggttc taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca
     9361 caacggaggc gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa
     9421 ttatacaaat aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc
     9481 cagccttgaa tttcacgtgg aatctagaaa caaattgttc atatacgatg ccggcaagcg
     9541 gcccgataat catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag
     9601 aagcaattgc accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac
     9661 tgatcaagat gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt
     9721 gcaaatcgcc agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac
     9781 ttagatttgg catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag
     9841 acacgcgact gctgttgttt gctttccatt tgtattccat ctttttcacc atacttctat
     9901 ttattgatgg acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc
     9961 agctatctgg gaactatttg cctggttcag tcgcaatgat tgctagttca ccttggaccg
    10021 cccactcaga aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt
    10081 ttccgtcaac gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt
    10141 gttttttgaa agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt
    10201 ctaaataagt gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg
    10261 ttttcgcagg gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc
    10321 cttgcgcatc ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg
    10381 tttctaaaat catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga
    10441 aatcaccttt tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca
    10501 tctcagctag ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt
    10561 cagcatcgat gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat
    10621 aggcatcatc tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca
    10681 ataatggaaa cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt
    10741 catctaatct tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg
    10801 cactgatcgc ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac
    10861 gtaaacgact tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct
    10921 gcataattca aactcctctc atttttcata gctattatag cattatttcg gtaataccgc
    10981 tttctctttc aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg
    11041 atcgacagga tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat
    11101 caagaaaaac gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc
    11161 cttttttaag tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag
    11221 taaagaatca atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa
    11281 atacatttgg acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc
    11341 atgttcagcc aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga
    11401 aggcagttgt tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat
    11461 cattcctaat ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat
    11521 ttcctctagc atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc
    11581 tagttgctca cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt
    11641 atctctgttt ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt
    11701 gattgtctga ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt
    11761 tccatcttct aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc
    11821 tgcatcaaac aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc
    11881 ggctgtaaat gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa
    11941 tccttcttcc atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg
    12001 cttttctatc agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt
    12061 tttacacaaa aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt
    12121 tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc
    12181 gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct
    12241 cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt
    12301 tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt
    12361 cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa
    12421 ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga
    12481 tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt
    12541 ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac
    12601 tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc
    12661 tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga
    12721 gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac
    12781 ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt
    12841 tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca
    12901 tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat
    12961 caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg
    13021 ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc
    13081 tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt
    13141 tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa
    13201 aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    13261 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    13321 tgaacaccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagatac
    13381 aaaacggaaa aatagcttct catattttgc cgttttttga gctaataccg aaggacagct
    13441 gattgaacac cgtttattcg gaaaaaggga ctgtgacacg acttttgtca cagcccctaa
    13501 aaaaaataat aaatttgtac tattctatga agcttcttct tcagaaaact gactgttata
    13561 aagatctgca tagaatccgt ctttttccat caatgtatcg tggtttcctg tttctacgat
    13621 cgaaccatga ttcatcacaa taatgttgtc tgcatcgcgt attgtagaca agcggtgagc
    13681 aacaacgaaa cttgtccggt tttccaatag gcggttcatc gctttttgaa tcagtatttc
    13741 tgttcgtgta tctacactag atgttgcttc atcaaggatc aggacatctg gatttgctaa
    13801 gaatgcccgc gcaattgtga ttagctgacg ctgcccttgc gaaatattgc ttgcatcctc
    13861 gtttaataca gtgttatatc cttctggcaa tttacgaaca aaatcgtcta cgtgagcagc
    13921 tttagcagcc tcaatgattt gttcgtctgt tgcatcgtcg ttcccatatt tgatattgtc
    13981 atagatactt cctgtaaaca gccaagtatc ttgcagtacc attgaaaact gtgcgcgcaa
    14041 ttcatcacgt gaaagatcac gggtatctac accatcgtaa cgaatactac ctccagaaac
    14101 atcgtagaac cgttcaagaa ggttgatcag tgtcgtcttc cctgcaccag tcggacctac
    14161 aatagcgacc atctcaccag gtttgacatc caagttaaaa tcagtcatta gcaagttatc
    14221 tggtgtgtag ccgaattgaa cattttcaaa acgaactttg tatggtgtat ctttttcttc
    14281 tggaaggcca gatttttcat tggacatctc ttcttcatcc aacacttcaa atacccgttc
    14341 ggctgaagca actgttgatt ggatagtgtt catcaaactt gcaagttgag tgattggttg
    14401 agagaactgt gttgtatatt gcaagaacgc ttgaacatcc cctaatgtca tgttcccatt
    14461 tgcaactttt actccgccta gtacagcgac gaacacatag cctagatttt taacgaaatt
    14521 catcaacggc atgatgattg ccgaaataaa ttgagctttc caaccagcgg catacagatt
    14581 ctgattttct ttttcaaata cttcctggtc tttttcagta tgattgaatg ttttaacaat
    14641 ggtatgacca ccatatgttt cttctacttg gttattcaat aaacctaaac ttttttgctg
    14701 atctgcgaaa aacacttgtg atttcggcgc tacgatcatc acgatgatca agcttaaagg
    14761 gatcatcaat aacgcgacta acgtcaattg ccagctgatt gaaagcatca tcacgatgac
    14821 cccaatcaat gtgactgcac tagttacaaa ttgagtcaaa ttttgctgca gcgtgcttgc
    14881 gatgttatcc atatcattga ttgctcgaga catgatatcc ccatttgtat gtgtatcata
    14941 ataggaaatc ggtacacgat tcattttggc ttccaaatca tgacgtaatt cataaactgt
    15001 tcgttgggaa acacgtgtca taataaattg ctgtaaaaag ttgaatacag cagaaatcaa
    15061 atacatcacg attactgtcg caatgatctg cgcgatttta tcaaagtcta tcggaaattt
    15121 tgttacttgt tggccttgtt gcatctgttg agcgccagcc attacacctt tgaatatttc
    15181 tgtggttgct ttccccaaga cctttggtgt ttggatttgg aagatcacgg ctgctattgc
    15241 tagtacaaat acagcgatga tcgcaaccat acgtttagac atatagccaa acaatcggcg
    15301 taccgttccc cagaaatttt taggcttttc accttttaca ccgagatttc tgccaggtcc
    15361 accatgtcct ggaccgcctg ctggtccatg agagggacgt gtgtttgttt tactcatgct
    15421 aagtcctcct ctcttagttg tgaatgaacg atttcttgat aagtttcgtt tgttgctaat
    15481 agttcttcat gtgttccttt gccaaccagt tttccttcat ctagcacaag gatcatatct
    15541 gcatccatta ctgtacttac tcgttgagca actaaaacgg tgatactttc tttcatattc
    15601 gttttcaatg cttggcgaag attggcatcc gttttgaaat ccaacgctga gaaggaatca
    15661 tcaaacacat agatatctgc tgttttgact aatgcccgtg caattgccag acgctgtctt
    15721 tgtccaccag agaagtttcc tcctccttgt tccacatggc tatctaagcc atcttccatc
    15781 tctgaaacaa aatctttagc ttgtgcgatt tctaatgctt tccaaatcat ttcatctgtt
    15841 gcatccggct taccatactg catattgtct cggatcgttc cagagaacag aactgctttt
    15901 tgaggaacaa agcccattaa ttctcgcaaa ttatattgag tcatgtcact aatgtccgtt
    15961 ccatttattt ggatcgaacc agattcgatg tcatataatc gagggatcaa attgactaag
    16021 gtcgttttac ctgaaccagt cccccctatg attgcgacga tctcgcccga cttggcttgg
    16081 aaatcaatat cttccaatgc aagtttttct gctccatgat atcgataatt gacatgattg
    16141 aaggccaatg ttgcgttttt tccagcaaaa gaaactgttt taggattttc agggtcatgg
    16201 ataccgattt tttcatccaa cacttcatta atacggtcag ctgaagcttg tgcacgaggc
    16261 accatgataa aaatagcgga aagcatcata aagctgatca agatctgcat cgcataagtc
    16321 ataaatgcaa tcagattacc gacttccaaa gtcatatctg ctatgtagtg tccaccaaac
    16381 caagtgattg ctacgtttgt accgctcatg atcaatgtca tcattggcat catcaatgcg
    16441 acgatcgtat taactttgat tgccgtattt gtataatctt tattcgctaa atcaaaacga
    16501 ttttcttcaa agcgtgtctt atcgaatgct cgaatcactc tgacccctgt caatccttct
    16561 cgaaatacca ggtttaaacg gtcagttttc ttttgcatac ttttgaataa tggaacagca
    16621 aagtacatga ttccgccgat caaaataatc attactggga tgacaactaa gaagattttt
    16681 gtcaactgat gatctttttg ataagcaagt atgctcgcgc caatcaacgt gattggtgca
    16741 ttgatcatca tacgtaaaaa catttgtgta accatttgga tttgagtcac atcattggtc
    16801 gttcttgtga tcaaagaggc cgttccatat ttatcaaacg catcttttgt cagactctct
    16861 gattttttat agacatccgt acgcaattgc ttacccagtt tctgtgattc acgagtagca
    16921 aaaaatgtat ttgcgatagc agctaagata ctgatcaaag aaaagccgat catcacaaaa
    16981 cctgtatgcc aaatataatc gacatctcct tgcgctaccc ctttatcaat aatattggag
    17041 gtaagtgtcg gcaaatataa atctgccagt acttggatca ccatgaaaac tgcagctgca
    17101 attgctgaag tcaaggagat ccgttttacg atttttagca tctagttccc ttctttctat
    17161 ttatatactc cgtttttcag ccaagaaagt ttcaaattga attcactgat tgcttgctct
    17221 actgagtaat cttcagatgt tgaaaataag cgatacgaat gcgcaattgt tgtaaataca
    17281 gtattcatca ctaattggat cagtaattcc aattcatgat cattttttac tttcagcttc
    17341 gtccgatcga tcaattcaag caactctttt gcatcttgtt tgtgttcttt atgtggatcg
    17401 tgaggcgttt tttggcgact cgtatgagga gcgacttttc ttgaggcacg gtaatccatg
    17461 ttcataaaca agttacgata aaaagaagca ttctcgcctt ccaaaatctc ataaatcatt
    17521 ttagaaaaat aggattcgaa tcctttgaat agatcaccat cggattcttt cattagttgt
    17581 aacatatctt tagagctgtc acgtctcatt gtctcaaagt aataaaaata aagatcctct
    17641 ttatcttcaa aatactgata gaaactgcct ctagggatat ccgctaattt gacgatatta
    17701 gcaatcgaag cttcttttaa gggtactcta gaaaactcga ttctcgcagc ctcgattagt
    17761 cttttttgtt tttctttagg caaatggaaa aatgtctgtg tcggcatatg gatcctcctt
    17821 tctttcaaga tgacacattg tcatatgacg ctgtgtcatt ataacacctc ctacctgttt
    17881 tgcaagacat ttgcatagtt tttttcatta tcatccgata cattaaaaaa gtgataagag
    17941 gaatccttcc atttgaatta atgagagttg cccattaaaa tagccacata ctaaacaaag
    18001 gatgagataa atggcccaat caattagtgt ccaagacttc aataaattgc ctctcgatgc
    18061 tgacagcatc gttttagata caagaaatac cttagattac acaaaaaacc acttaagcca
    18121 ctcgctttct atccccgctg ctatcttgcc aagacaatta aaaaatctag atccaaagaa
    18181 actttattat attctaagct atacaggcag gcgatcagaa gcattagccg atcaacttac
    18241 tgctaatggt tttcgtgctg tttatatcat tggtgggatg gaagctcttc agtcgagtgt
    18301 tgcttgaata ttgcttgtta gattcaaaag aggctgtgac aaaaaatcgt gtcacagcct
    18361 cttttatacc ctgtcttttt ttagttattt attcttcaac cttatcaata tgacttgata
    18421 gccttttctt tttctcatag ttataacaat catagcaata aaaggagaag gtaagcatat
    18481 ggcatctgtt ttagtagtca aaggtcatcc cctaactgca gaagaatcgc gcacagttaa
    18541 ggcattgacg tcttttttaa caagttataa agaaaatcat ccagatgatg aagtgactgt
    18601 tttggaactt tatcgcgatg atattccgga aatcgatgaa gaattattat caggttggga
    18661 agcattacgt gctggtgcag aattcacttc cttatcagaa agtcaacaac aaaaaattgc
    18721 tcgtttcaat gaattgactg atcagttcct tgctgctgat aaaatcgtta tcgcaaacgc
    18781 cttatggaat ctaaatatcc caacaaaatt aaaagcttgg ttcgataccg tcaatgtagc
    18841 tggaaaaacg ttccgctata cagaaaacgg gccagaagga ttagtgacag gtaaaaaagc
    18901 actgcacatc caatctaatg gcggtgtata caacggacaa gactttgcct ctcaatatgt
    18961 aaaaggtatt ttaaactttg taggcatcga tcaagtagat cagctattca tcgaaggaat
    19021 cgattatgac cctgaccgtg cagatgagtt gatgcaaaat gcattagata aagctgctgc
    19081 tttaggtaaa tcattttaaa aattgagata aatgcataca aaagagacag aggaaaaaat
    19141 cttcaaagca gcatttgtac cttggatatt ttcccatctg tctctttttg ttattctttc
    19201 tgctttttca acggattttc ttcataaaga gaagagattg ttgtgtacca ttcaaaaaca
    19261 tgagtaatca ctactttagc tgctgcataa acaggaatcc ccaagatcac accgatgatt
    19321 ccgaacaatt tcccagaagt cagcagaact aataaaatcg ttactggatg gattgctaac
    19381 tgattcccta gtactaatgg agagatcaag cgtccttcta ttgtctgctc tactgcaaaa
    19441 acgatcagta ctttgatgat catgaatgga cctgctacaa tggctaaaaa gatagccgga
    19501 atcatcgcta aaaatgatcc taagtaaggg atcaggttca aaaaacccgc aataatcccc
    19561 aacgtgacag catactctaa accaatgacc gcaaaaccaa tcataaacat gattgccaca
    19621 gcaaaagcca cagttagttg tcctcttata taagaagaaa cttgactatt catttcactc
    19681 agcacattca acgtcggttt ttgcatgcgt gtcggtaaaa atttgacaaa ataaggtgct
    19741 aattgtttac cgtctttcaa tagataaaat aagataaacg gcatcgtcag cagagccact
    19801 aaaatagtcg ctaccgcgcc gacaaaactt ccaagactct gaactgtact cttagatata
    19861 tcttggatca tatcgcctgc cgaattcatg aacttatcac ctgctgtttc caactgttcg
    19921 cgaaattggt tgaacagcgg gtcccgcaaa atctcggtta atttattatc aatcgtatct
    19981 acatattgag ggaagttact gatgaagctc actgtttgtt cctgaatctt agggatgatc
    20041 actacgctcc cccatgcgat tagtgcaaca accaaaacaa acagcccaat gatactgtaa
    20101 agccttggta cttttcgctt ttcaaagtaa tcaactaccg gattcatcaa gtaatacaag
    20161 attcctgcta aaatgatagg cagacctacg attgctaaaa attgccagat tggtgcaaac
    20221 aaatatgaaa cttttgtaaa taaaaagacg atcagcaata ttaacaatac aacaagtaag
    20281 gctgtgacta cttgattatt taaaaaccag cgccaaaacc acgataccgg tctgttcttc
    20341 ttattttttc cttccataat tcctcctgtt agttataaaa aatttggctt cccaaaccaa
    20401 tagcaggcag tctatgcgga tgaagataga ttccatttgc aatttgtcct tcttctggac
    20461 aagcatcaaa aatcaatgat acgtgagcta ctgtctctaa attcccattg gccatcggac
    20521 cgacataatc gatcgtatat acctgatcat caaaagcaat tgttccgcct gctttcaaag
    20581 aaaaaggttc gtcttttgat acttcttgaa tcaccgaata ttttcgtaag gcagctgttg
    20641 ctgttttatc gaacaaaatc agcatgggtt cttccttgtt tattgcttct gtcccaattt
    20701 ctgtaatctt tgcctgaatc attgcactct ctcctttaat tgctttcaaa aacagtataa
    20761 cacattctca ttctttagcc gtgtggaata attatgtgat agaataaaaa cgaaaactat
    20821 ttcattacaa ttaggaggaa tcagctcata tgatcgaaaa gataatttta ggaacctata
    20881 cgcgtcgcga aagtaaagga atctatacga tcgaccttga tacagaaaaa gaagaattag
    20941 ccaacctttc attagctgtt gaagaaaata gccctaccta tgtagcaaaa agtaaagctg
    21001 gcaatcttta cacagttact agtgtggatg gattaggtgg tgccggtgcg tacgataagg
    21061 attttcattt tttgaatgcg gtaacagagt ctggcgctcc tttgtgttat gtagcggttg
    21121 atgaagcacg tcaattacta tacggggcga actatcataa aggtgaagta aatgtctacc
    21181 atatattagg agatggcgaa ctgaaagcag cagattcaat attccatcaa gaagcgacag
    21241 gtcctcataa aaaccaagat catgcgcatg tccactatac ggatctgact cctgaccaac
    21301 gattagtcgt atgtgatctt ggtacagatc gtgtgtatac ttatgatgtg tccgaaaacg
    21361 gcaaattgaa tcttgtaact accttcactg cagagccagg aacaggccct cgccatcttg
    21421 tctttcatcc aaatggacaa ttcgcttatt tatttggtga attagacagc acagtccgcg
    21481 tgctttctta taataaagaa acaggtgctt ttgaagaatt acagaaaata tcgaccttgc
    21541 cagaagaatt cgacggtgaa aatggagggg cagctatccg catttcaaat gatggcaagt
    21601 tcctttatgc ttctaaccga ggacataact cgattgctgt attcgcagtt tttgaagatg
    21661 gttcccacat tcaaaacaag caaatcatct caacagaagg ggattttccc cgcgatttcg
    21721 cactagatcc aagcaataat tttgtcgtgt gtgccaatca gaacaccgat aatctgacat
    21781 tatatcgccg tcatcctgaa acaggattac tggaattact gaaaaaagat gtctatgtac
    21841 cagaatgtgt ctgtgtttat tttgatcgtt agtaagaaaa ggagaatgaa atgaatcagc
    21901 aagacgtatt agacagacta cgggaagaac taaatattcc tttctttgat gggaaaatcg
    21961 aagataaaga gtatagcgaa gaggaatatc agaaactcaa agccgattta cagaactact
    22021 ttgaacaata tgtacagaat gtagaaaact aatcagcttt ccatactatc tgaaaagaac
    22081 atcaggacaa gacttttccg tctatcctga tgttcttttc tacaaataaa aaaccgccga
    22141 gatggttaga ctcgacggaa aaggagtatt tactcgattt tgagtaagta caaggttgtt
    22201 gttggttata ggtatgtcat tatcataaag gattttgatc actctatcat aaaggtaata
    22261 ttacatttat attagataac tctagttttt attgttatca aagatttctt ttattttttc
    22321 aacaacaaat tagtcatttt ttggatggca gctgtcaatt ggtctgtcgc ttggtcttgt
    22381 tttgcttcag atgtattgaa tagatcttca tctacccctg attcaagatc gatactttgg
    22441 atcatttctt cacagcctgc tacatatgaa agataagctt tttcaaaatt tttatgaatg
    22501 cccattgctt ttgctggtgg acgcaaagta ctgactttct taagcattgc ccgatatttt
    22561 tttgttcctt cttcaaataa caggatgatc tcttcaagag tcgatttgtc cagttcactt
    22621 gtttttcctt catcaattgc gtttctgatc acttcatatt tcggatgcat tccttctccg
    22681 attgcttctg tttccttgac gatttgatcg atggactgca cataatagcc aatattcggt
    22741 aacatgttcg ttttcctact ttctccattg ttgttattat ttattctact tgtttttatc
    22801 ctcaaatgac aagtacttga aaaaatttaa tggtttctat acagctcgcc attcttattc
    22861 gtgctcaaag aaaccatctg ctaaactata ttgtttccct gcgacaatgt catattggat
    22921 caattgatat tcgtcgtaaa tctcttgttg cgagcgattg aattgcgctt ctttatacca
    22981 ctgtccgttt tgatagtaca gttctcgttc aaacgccgga agttcctgtt ctaatgataa
    23041 aagcagctga taaaagccat ttgttgtctg attcgtctgt tccattagat tcgctgcaaa
    23101 ataaaacgga ctggtgattg catcgtgagt cgtttgcttt tctaattctc ctttactgtc
    23161 gaacataaga aattgtgttt catgcaatgt ttgtttgtca ttttttgctt ggatcgtatc
    23221 tgaatagatc cctggaagat gatcccccca aaaaacaact aaagtccttc tagaaagatt
    23281 cttcaattct tccgtgaact gcttcagtgc cgtgctgcta taggaaatat cttgcagata
    23341 attctctaag tcaagtgtcc cacttccatc tgaagttttt gcaggatagt ctagattatc
    23401 atactttcca tcgtagggca tatgtgtctg catcgtgact aaatgaatga actgaggtgt
    23461 tttttcttcc ttgagaagtg tcattacttc tttgtaagct gactcatcag aaatatatgg
    23521 attattctct atcgtatccg tataggtcat cgtacgctcg ctgatgaact ggtcaaatcc
    23581 taatgtctga taaacatcct ctcttttata catcgaagta ttgtatggat ggatcgctgt
    23641 cgtctggtat gattgtgcgt ttaacgctga aacgatagaa ggaagctgat ccatttttgg
    23701 tacaagcatc gtgtaagggg tggtgagttg cgcattaaat aacgccatag aaaaaccagt
    23761 caatgcttca aattcgatat ttgctgttcc accgccatag ttttgagaaa gcatattgcc
    23821 actatatgtt tgatcagcta cttcataata atctgccaat ggctcaccag atacttctac
    23881 gccgtttaat cgactaggat ctgagaaact ttcactcatc acgaacacga tgttcggcga
    23941 ctcttcctct acagttttat ttttttcatc ggctagcttc tggtattttt cagtgatttc
    24001 tttgatcttt tctttcgaat atccttcagg ttcatccatt ggttctacct tcagattgta
    24061 caaaaaccca ccgataaaac cagtgttata gtagttcatt ttctggctgt aaggtatcca
    24121 tagtgccgtt ttattatatg cttttcgcaa caaattatcg ggattgttga aattactgat
    24181 ataccctagc agtccaattg ccacaagcag tgtcgttaat cggatcaatt ggaatcgcct
    24241 tccttttttc aggcttcgaa acatgtacca gcaaaacaat cctatcacgg ttcctgcggc
    24301 agctaagatc actgtaaaca gcatagtgcc ggtcatatct ttcagcaatc cgatttcagt
    24361 gatcatcttc aaatcgtctg gataaatcgg ttcttgccga taactcattt tcatataatt
    24421 tgcaaaacct aatacaccga tggctacgac atacgtaagg gaacctaaag ggacagatcc
    24481 tgctagtgca atcagaaaaa taagaataat cagcaagacg agacaggcta agaagaattt
    24541 ttccgtatgc caagaaaaag caaatttcaa cgcaaggtct acggatagat cattttgaca
    24601 ccactgcaaa aataaattac taaaaacaac aagaaaaaag atactgatga ttgctgctgt
    24661 tccttttaaa atctttttct gtttggtcga aagtctgctt atatatcttt tcaaatgctt
    24721 gctccttcat ttctatcaat aaaagaatgt tgcttctctt attttagttg tacgaaattc
    24781 gaaagtaaag aacggaaaga agaattaatg aaatccaaat attctctttc tttatggcct
    24841 ttctggtttc tttaataagc ggacctttcg tactccagaa attttttcta gctgttgttt
    24901 cacttgattg agctgttcaa aatcagtact gtctatgtca atcaatatgt agacatgctc
    24961 attcttatgg ttacgcgata tatctgcgat cgctagctcg ttctcattca gcagagaaaa
    25021 tatctctgct aatatattgg ttttcttttg ataaaatata gtgaatcgaa acggcgcttg
    25081 gaaaaacatt cgtgcggatg gataattgac cgcttctcta accgtaccga acaaaagata
    25141 atcttttaag gcttcaactg ccaaacgtcc gctgtctgcc aaagcttcct gagtcgatcc
    25201 ccctagatga ggcaacataa gtatacggtc attttctaag aactcttctt ctggaaaatc
    25261 agtcaagtat ttagccagat gttgtttttc caatgcttgc ataacagcat atttatcaac
    25321 aatttcactt cttcctacat tgataagtac agcatctttt ttcatttttt ctatattcgc
    25381 ctgatcgatc aatccttttg tatcttctgt caacggcagc atgactacga tgtaatccga
    25441 ggtagaaaga actgtttcta aatctgcttg ccgaacataa tccaactggg catcgcggat
    25501 agaataaccc aagacatcca tccctaagct atagcaactt agcgctactt ttgtgcctat
    25561 cgctccaaga ccaagaagac cgatggtttt gccttgcaat tctcgtccca cataagcact
    25621 tcttttgttt tctgcctgtt ctaaaatatt aggacctgtt agtgtttgta ccatacgact
    25681 tgcttcgatt atcggacggg aagataagag caaacagcac aaaacaagct cttttactgc
    25741 attagcattt acacctggtg tattcatgac gatcgtcccg ttttcagaag ctttttcaac
    25801 gttgatcgtg ttcaccccaa caccagctct tgaaattgct agcaaatctg gtgtaagcca
    25861 ttcatctttc actttcgtac tacgaataac tattccttcg gctggcaaat catctattat
    25921 tttgaaatat tccccttgta acttttctat ctcttgttct ggaatagcat caatcaaatg
    25981 aacgtgatac ataaaacccc ctccttacta acaagtatct cgaaatttct gcaattagac
    26041 aactgtttac catttgactt cctagaagca aaaagagcag ttagagcaga gtaggctgag
    26101 tgataaaaat aaggctatga caagttactg ccataagcct tattttcgag agcaaaatat
    26161 tgagggtgag atatcttgat aaatggtgtt ccgttagctg acattgataa taagactaaa
    26221 tttcaaaaaa gttgaaaagc aaatttcgtc aatctcttct tatttcttaa aactaagtgg
    26281 ctttttcaca acctcttttt atctaataac cactgtttcc aactcaacta attttgccca
    26341 cgtttcaatt tgatctgttg ttagatttag cgagacaacg gtatgatgac cgccacctgc
    26401 ctgaatccat gaatggacac cttcatggaa attcggttta gttttccata acacacgtgc
    26461 aacaggtaaa ttaggtgctt cagtcgttgg ttcaaaggct tctacttcat tgatcagcag
    26521 tttataatgc gtaccgaaat ctgccatcga tacgactacc ccttctcctg ctttcccatc
    26581 aaatacaaga cgcgccggat cttctctatc tcccattgat aaaggagaaa caacgattct
    26641 tggtttcgtc gcagcaagcg tcggatctac ttccatcata tgggattcca agattgcttc
    26701 ttggccacta gctagctcgt aagtataatc ttccatgaat cctgtatctt tccctcgagc
    26761 catgattttc agcaaccgat caatcgctgc agttttccag tcgccttccc cagcaaaacc
    26821 gtatccttct gccatcaaac gttgtacagc tagtccaggt agttgtttca ttccatacag
    26881 gtcttcaaaa ttagtcgtaa aagcgttata gcctccttct tctaagaaac ggcgaatacc
    26941 gatttcctgc tgagcttgta cttttacatg ttcctcccat gtcttttctt cataatcacc
    27001 atagtcgaag gtatacaatt ccttgtactc ttcaaataat tgatcgactt cttcgtctga
    27061 tacgcgatcg attacttgta caagatctcc gataccaaaa taatcaacag tccaaccaaa
    27121 ttgaatctgt gcttccactt tgtctccttc tgtgacaccc acgtttctca tgttgtcacc
    27181 aaaacgagcc acttttattc caaaactttc attataggct actgcaacat ccatccaatc
    27241 agcaatctct ttttggatct caggtcgttt ccagtagcca acgacgattt tattttgttt
    27301 atttaatcga gcattgatga acccatattc gcggtcacca tgagcggatt gatttaaatt
    27361 catgaaatcc atatcgatcg ttttccaagg aatgctttcg ttatattgcg tagctaaatg
    27421 aagcaatggt ttttgtagaa gctttgtccc gcggatccac atttttgctg gtgagaaagt
    27481 atgcatccaa gtgatcacac ctgctacttc ttctcgataa ttgacctctt tcatgatttt
    27541 tgtgatcgta tcagcagtaa ctgctaattc ttgaaggcgg attggataag gcaattggcc
    27601 attttcattc agttcatcga ccatttcttg tgcatgtttc tttacttctc gcagtgcttc
    27661 ttccccatat aaatgctgtg atcctacaac aaaccaaaat tctttttctc caatattcaa
    27721 cataattagc cactcctttt atttgttttg tccgtaatac gcattttttc cgtgttttcg
    27781 taaataatgt ttatctaaaa tccgttgagg cagctcttct gaaaatgaat tgagctgtct
    27841 ggtaaataaa ttcatcttgc atacttcatc tagtacgact gcgttcatta ccgcactttg
    27901 cgcatctttc ccccaagtaa atggaccgtg tccgtgtaaa agtacccctg gaattgctaa
    27961 gggatcaatt ttgcgttctt tgaatgtttc tatgataact tttcctgttt catattcgta
    28021 cccgctgtcg atttcctgtt gtgtcaaaaa tcgagcacaa ggtaccgatc cataaaaagt
    28081 atctgcatgc gtcgtcccca tcgcaggaac atccaatcct gcttgtgccc agatcgtcgc
    28141 ccatgtcgag tgagtatgga cgattcctcc gatttgagga aatgaacgat ataaaacagc
    28201 atgagtaggc gtatccgaag agggattgag tttaccttca atgatatgat tgtccaagtc
    28261 cacaacgacc atgtcgtctg ctgtcatctc ttcataactg actccgctag gtttgataac
    28321 gaaatatcgt gtttcaggat caaaagcgct cacatttccc catgtatatt tgacgagtcc
    28381 gtgtttaggc aagtcaagat ttgcttggta tacttcttct ttcagttgtt ccagcatttt
    28441 ctatcactcc tttttcttgc aattgatgaa tcgctgtttt ttcaatgtcg atcccctcac
    28501 ggtaacgtct cataaatatc tgatagcctg ctatttcttc tgctgaaggt gtgatggtga
    28561 ttccttggtc ttgtccaaac acgtcttctg ccaagaagtt ttctaaagac ttcccatgtc
    28621 gatccatcat ataggctgct aataacgcga ttccccacgc tccaccttct ccggctgtgt
    28681 ccatcactgt aactggtgcc tccatagcag atgctagaat ccgctgtcca acttctggtg
    28741 ttttaaaaat ccccccatgc cccactaaac gatcaatttg gacattctca gatttcaata
    28801 tatccattcc gattctcatt gcaccaaaag cactggctaa atggatccgc atgaaattcg
    28861 ctaaatcaaa acggctacct ggtgtacgta caaataatgg ccttccttca tttactcctg
    28921 taatgttttc accggaaaaa taaccataag aaagtaatcc cccgcaatcc gtatcgcctt
    28981 ctagcgcttt gttgaataat accgtgaata atttatctct gcttatctcc atacccaaag
    29041 aattggtaaa ttcttcaaaa atactgaccc aagcattgat atccgaagag cagttattcg
    29101 tatgaaccat gcctactagt tctcctgaag gtgtagtgac catgtcgatt tccggatgga
    29161 cattttctag ttctttttct aatacgatca tagcgaaggc ggaagtacca gccgaaatgt
    29221 tccctgtccg tttggctaca ctgttcgtag cgaccatacc ggttcctgca tccccttctg
    29281 gcggacaaac cgggattcct ggtctcaaat ttcccgtagg gtcgatcaga taagcccctg
    29341 attcgcttaa cttgccagct tcttctcctg ctactagaac tttaggcaaa atgtgatcca
    29401 agtcaatcgc tacgtgcgct ttttcgatta actgaccaaa aatatttttc atttcttgat
    29461 ggtaggtttt cgttgaagga tcgataggaa acatgcctga tgcatctcct acgcctagta
    29521 ctttctcacc agtaagctgc caatggacat aacctgcaag cgttgtcaaa aaattaattt
    29581 ctttcacatg ttcttcttta ttcaaaatag attgatacaa atgagcgata ctccatctct
    29641 gcggaatatt gtattgaaat ttttccgtta atattcgttc tgcttcttca gtgattgtat
    29701 ttcgccaagt acgaaatggt actagtaact gttccttgct atcaaatgct agataaccgt
    29761 gcatcattgc actgaaccca atcgacccga ttgtcgtaag attctcgcca tagatttcga
    29821 atacttctgc tgccattttt cggtaactga cgcgcagtcc tttccaaata tcttctaatg
    29881 aataggtcca gataccattt tctagtttgt tttcccactc ataactacca gcggcaatag
    29941 gagtaaaaga tgaatcaatc aatacagctt tgatcctagt tgatccaaac tcaatcccta
    30001 aagatgtacg accatctatg acatctaaca tactttgcgc atttacttcc ttcataatga
    30061 tttaactccc tccttctcgt caaacctgat caaaccgtct ttttagtagc gcttacaatg
    30121 attattgtat acttttgtac gtacatgtca agcggttatt tacaagaata caataaaaaa
    30181 cacttgtttt gtacgtacat aagttataaa atgaaattgc ttacaaaatc tgatcaaccg
    30241 ccttctcgaa attctagaag gagagaatac ttatgaaaaa agaaaaaaaa gtttcctcat
    30301 cgtttattta tttttttggc gcatttggcg gcattctatt tggatatgat attggtgtta
    30361 tgaccggcgc cttacctttt ttacagcatg attggggttt ggcaggtaaa gcttctttga
    30421 taggatggat cacgtcttcg gtcatgttag gtgcgatcct tggcggctct ttatctggtc
    30481 ttctttctga taagcttgga cgacgaaaaa tgattcttct ttctgcactt atttttatgg
    30541 ctggttctgt cctttctgca tctgcccctc acaatggttc ttattttctg attgcagcaa
    30601 ggattcttct aggtttagct gttggcgctg catctgccct agttcctgct tatatgtctg
    30661 aaatggctcc tgctagacta aggggaagat tgtcagggat caatcaagtg atgattgctt
    30721 ctggcatgct cctttcctat gttgcagatt atttgctaaa agtgttgcct gaaacgatgg
    30781 cttggcgcgt gatgttaggg ctcgctgctg tccccgcgct gattttattt ttcggtgttt
    30841 tagctctacc agagtcaccg cgttttctta tgcaatctgg ccgcttggaa gaggcaaaaa
    30901 gagtattgaa ctatattcgg acaccaaaag aagctgaaca ggaattcgaa caaatccagc
    30961 tgaatgtcaa acaagaaaag acaaccggaa cttcttggca tactttgttt ttagaaaaat
    31021 atcgttcctt agtcattgcc ggtattggtg tagcagtatt ccagcaattt caaggagcta
    31081 atgctatttt ttactatatt ccgctgatcg tagaaaaagc gactgggcat gcagcgagcg
    31141 atgcattgat gtggccgatc atccaaggaa tcattttagt tgctggttct ttgctctttt
    31201 tggttattgc agacaagttc aatcggcgaa ctttattgaa aataggcggt agcgtgatgg
    31261 gactttcatt catcctacca gctgttttag gaactgtact ggatgctcat acaaattcgt
    31321 tgttgattct cttgttttta tgtatttatg tcgcatttta ttcttgcaca tgggctccac
    31381 tgacatgggt aatcataggc gaaatattcc cactagctgt ccgcgggcga gcatctggcc
    31441 tagcctcttc ttttaattgg attggttctt tcctagttgg cttgcttttt cctgtgatga
    31501 ctgcttctat gtctcaagaa atcgttttcg gcatttttgg gatcatctgc tttttagggg
    31561 ttttatttat tcaagagatc gtaccagaaa ctcgtgggaa atctttagag gaaatcgaac
    31621 aaagtgcatc taaaaaaact tatcctaaac gtatttctat ccatcattct taaagaggtt
    31681 gtgacagaag tggtcagctt caagaaataa gaaggaaccg cttagtttcc agtcacaagg
    31741 aacaatttca aaaaacagct tctcatgttt gatgaatatt gtgacttgtg accagttggt
    31801 aggagaacac cgtttagtgg agaggtggtg aaagaaacgt tcagccttga gcgatgacaa
    31861 aagctctcac aaacattgcg acgagtaacc gtaggagcaa taagaggcgg taaaaacccg
    31921 tctcttattt ttcgttttct ttcactgttt cccgttcaac tagtacaggt tcatagagaa
    31981 tatttggctg tgcctgtcct ttttcaatcg ttgtgacgat ccaccgtgcg gcatcttgtc
    32041 ccaacttttc ttttggatgc gtcaatgttg tcaatttttt attccctaaa tggcttaaag
    32101 aagaatcgtc tgttcctacg atcgataggt catctggaat atgatagcca ttttctgtca
    32161 acttatctag cattaaacta gctatttgat cgttatagca aatgatgcca tctaaagctt
    32221 gttcttcttt caaccgttgt actgtttttt cagcgattgt ttctcgtgtt tccgtggtga
    32281 aagtaatgat atcttccgtc aaaaaacgca cattatactg ttcacacgcg ctgataaaac
    32341 ctttcatcct gtattttcct tgaagatcgt cgatttttgt gatgaacaac agacgctgat
    32401 gtcctttttt tatcaaatat tctgctgcca taaaaccgac tttcacatca tctaaacaaa
    32461 tataagaggc atttaactct tcataagcag cattgatcat caccatcgga atgccttgtt
    32521 ctctcaaagc aacataaagt gctaagtttg ggttatactg attacttttc gtaggctcaa
    32581 cgatcaaacc atctacccca taagcaatca ttttcttcaa acactctttc tccaattcgt
    32641 gatcgttatt cgtactggct aaaagcaatg aatacccaga ttctcgtaat tcattttcga
    32701 ttcctcggat aatcgacgga aaaatataat cagagagata tgtggtgatg acaccgatcg
    32761 tttttgttct gttcaatggt gttttttctt tcttatagct gtcatctaca tatgtgcccg
    32821 accctttttc tttacgcaga tagccctcat ttaccaacac agcgattgct tgccgaatcg
    32881 tatgtctgct gacgtcatat atttcttgca gcttgaattc tgagggaatg gttgtattta
    32941 cttcgtattc ctcacgaagg atcttttgtt ttatctcttc tgcaatatct tgatattttg
    33001 ccatttgcct tgcccctttt tatttgtacg tacatatatc ataacgagtc tgcaaggttt
    33061 attcaagcaa aaatcaaggt ttcttgtgct cataaggcat acggaacagg ttcaccaaat
    33121 tctggaaatc cttgctcatt ccacgtaaag cattgcgcgt tcgcgtgccg attaggattg
    33181 tctaacggat cccctttttg gttcttctct ggtcgagcat gatagatcaa aatatcaaag
    33241 cacccgtctt ctgaaaccgt aaacgagttg tgaccaggac caaattgctt atttttttca
    33301 gaagaaacga atactggctc cgataattta tgccatgatt cttttaataa aagattcttg
    33361 tgctcatcag cccacagcag gcccatggca tagttttcat ctgttgcact accagaatac
    33421 gtaatgaata ttttcccatt gcgagtaagg actgctggac cttcatttac tgaaaaaccc
    33481 cgtttttccc attcatactc aggtatagaa aggaggatct gcttcccctt taacgtccaa
    33541 ggattatcca tttcggatag ataaagatta gaattcccag taatttcagg atcttgttgg
    33601 gcccacacat aatatagctt tccttgagaa gagaaagttg ttccgtctaa actgaaagtt
    33661 tcccttttcg tttttatttg tcccttttct atccaatctg tattcattgg atctatatgc
    33721 tgattttcaa gtacgaacat gcggtgatga tgagattcat cacgtatagt tttatgattg
    33781 cttgcagcaa agtaaatata ccattttcca ttgatataat ggacttcagg tgcccagatc
    33841 aattcactca tcggtccttc ttcatgagcc tgccatacaa tcaggcttgc agcatgttct
    33901 aattcattca gtgactttgc ccttcttaat tcaatcactt ggtagcctgg aaccgatcct
    33961 gtaaaataat aataaccatc tgtatgttta tatacccaag gatctgcccg ttgccgaacg
    34021 ataggattat tataaagtgt cttcatcata acaaatttcc tcacattttc tttattctaa
    34081 ttacattcca agagtatttt tctatcccta catttaattc gtcatgtttt tctattgaaa
    34141 aatagtttat ttcaggtttt attttttctg gttcttcacg tgtatttttg tccaacagat
    34201 cgctactggt taatgtgtag tgctctcctt gatctagcaa attgaaatca tgttcaatcg
    34261 ttatcttttg ttgttcattt gtgcgattga tcaagaaaat agtcagctga ttgccgttat
    34321 ctacgatcac actgtccaca taaggaacat tttttatctt ttcacaatca taaacagggc
    34381 aaatgatttg ttgtttcaat acttgacctt tagcatattt cgataagtgt tgcatcacat
    34441 aataagttgg attcctccat gcttgtcctt tagcatctgt cagtattaaa ggtattgtgt
    34501 ttactaacaa tgattgacaa ctaatcttta cgcgatctgc gtgtcgtaaa atagccagcc
    34561 ctaatgaacc aaataataat gtatcttcca tagtaaagta acaccaatcg atgggactac
    34621 cgacttcaaa atccctatgt cttttttccg gttgggcatg tacattccat tcatcaaacg
    34681 ctagatacat tgttttctca cttctcaaat atccttttac ataatcacaa gtagcaacga
    34741 tttcttcaat ttgacgatcg aatcgataag cttttgcaag gtatgtcgct gtatcatcac
    34801 gctcattttt aggaggttta gtcaagtctt cttcatcata cttatcaata tagttatgca
    34861 aagctaaata atctacattc tcataagctt cttctaatgc gatacgatcc cattcagggt
    34921 acgatggtaa acgtggcgta gagcttccaa ctaagattgt ttcaattgaa tcatccacta
    34981 acttcatgac ttttgaggct tcatttgcta acctaccata ttcataagca ctcttagccc
    35041 ccacttgcca tggaccgtct agctcattcc ctaaacacca taatttaacg ccaaaaggct
    35101 ttttttgccc attttttatt cttaaatcac tccagaaggt tccttttgga aaattacaat
    35161 attctactaa attttttgct tcatcaattc cttttgttcc taaattcacc gtgaatatcg
    35221 gtttcgcttg tacatgactg atccatttaa aaaattcgtg gagtccaaaa gaatttggtt
    35281 ctatacctcg ccatgctaaa tcaatttttt ttggtcgttg ttctttccta cctacagtat
    35341 ccacccaatc ataaccagat gtaaagtttc ctccaggata gcgaatgaca cctaattcta
    35401 attcctttac ataagagagg acatcttgtc taaacccgtc accatcagct gtttcatggc
    35461 ccggttcata aatccctgta tagacgacac ttcccatatg ctcaacaaat gagccaaaca
    35521 gccgagaact gatctctcct accgtaaaat cctctctgat ttttactttc acttcttcgt
    35581 ccaacagctt tccctacctt tcatttccta tagtcaagat aatagataca agtttccata
    35641 cgccaccagc aaagataaaa ataaaaggag ggaaaagaaa tatgagcagt acaaatccta
    35701 ataacaatac gcaacaaaga atatttctca aaaaatcaaa aaatactttc catgcacttt
    35761 gttcaaaaat ttgtaaaaat gtgatatctt tgttttcaga aatcacatat atccaggata
    35821 aaagaaacat ggaataaata agtaaaacta catatccact aattgaaagt acgattgtct
    35881 gttcttctaa taattgatta acaagaaaat aaataatgat cgttaacaga ctaaggaaca
    35941 agttcttacc aaaatgttgc tttattcctt gaaagaaaaa ataaatgata tttcctgatc
    36001 cctcaccttt ccattgcgcc attacataaa aaacagcaca tgtagcaggc acgatagtaa
    36061 taacaggcaa acacacgatt acccagagaa tacctaataa gatgtaatca gaaaccacaa
    36121 tcaactgtcc ataaaattta gtctcaacta ttttcatctt cctcactctt tcattccaga
    36181 catagctgtt ccttgaacaa aatatttctg tgcaaaaata taaaccacta gtaatggcag
    36241 cattgaaata accgttccag ccattacagg tccataaaaa gaaatgttct gccctgtaaa
    36301 taaagctaac cctgctggta aagttctcat ttttgaacta cttgtcaata tcaatggata
    36361 aagcaagtca ttccaactat tcatcagcgt gaaaatccct aatgataaaa gagtaggttt
    36421 gctcaatggc aacatgattt tcaaaaaaat attaaactca cttaatccat caatgcgtgc
    36481 tgcctcttcc aaagaatctg gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa
    36541 tgcagttgtc atacgaggaa tgatcaaacc tgcataagta tcaagcatgc caagcaaatt
    36601 gacttcaata aatagaggaa tcatgaagac ttggaatggg atcatcattg tcagcaaaac
    36661 gaaatagaac aaaactgatt ttcctttgaa acgcatacga gcaaatgcat accccgcaag
    36721 agaatcaaag aatacagaag tgatcactgt ccctccagca aaaatcactg tatttttgat
    36781 gtaatctaat aaagggatcg ttgtccaaac cttaatatag ttatctaggg tatagctatc
    36841 tgcaaatatt ctaggcggaa atgagataat gtctttttcg tgtttaaaag aagaaataat
    36901 cagccacaaa aaaggaaacg caataaccaa caatataact agcatactaa tcgttcgaat
    36961 gattttttta ccactaactg cttttcgact attcacagta actgtaatcg agctttctgg
    37021 tgccatacag gtttccctcc tatttcattt gtttttcttt ccgtgacatg tacacattag
    37081 tggagatagc tatagttgca acaatgataa acaaaaccga agacagtgct gatgcatagc
    37141 ccaattcata aggtgctgta aatcctcgac tgtaaatata ttgaacagct gtttctgtct
    37201 taaattgtgg gccaccagcg gttgcaacat atacttggtc aaacacttgc atcgaaccaa
    37261 tcagattcgt taaaatacaa aaacctaagg agggaacgat ctgaggtagc gtaatgttaa
    37321 aaaactgctg tcgatttgtt gcaccatcca tttgagcaga ttcatacaaa gagggagaga
    37381 ttccctgaat agcagtcaag agaatcgtca tagtaacacc aaaattcttc caaacagtca
    37441 tgatggctac cgtcggcata gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc
    37501 cgacttgtga aaacaagtaa ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa
    37561 taatcgcaat tgacgtcaat gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac
    37621 gattgaatct ggtattttta gataatgcta ctgctgctaa taaaccaacg ataatttgtg
    37681 ttggcgtttc gagtacggtg aaatataaag aatgaatcaa cgaattaact gctcgttcat
    37741 cactaaatat acgtgtgaaa ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat
    37801 tcaaatctgt aaaactaata gcaaatgttc ctaaaagcgg tatgacagta aataataata
    37861 aaactaaaaa agatggagtt aaaaacaaat acgcagcacg atcaggacga ttccaatatt
    37921 tggtgaatcc gttcatatag tccacctcat ctttcatttt gataaaatat acaatcttgg
    37981 tttaaacgaa tctgaataag atatccgaac aatcgctgtg atttttgcaa taacacctat
    38041 ctgcaaatac taggtcgatc attcagatat ctgtatcttg ccaacagtct atcctgtata
    38101 aagttctgta ctatttttgc agtaattgat tgatggcatc atctgcatcg ttcatgagtg
    38161 tctcaggatc ttctcctgct aataattttt caatcagtgg attgatgata tcgttattga
    38221 tcgcagagat ttgggtaaaa cctggtaaat atggctctgc atagtccatt tgtttcgata
    38281 attcagatac aataggatca ttttttactt cttcatcttt tgctactgaa tccaagtagg
    38341 caggaaaacc attttcgata ctccattttt taccaatttc agttgtattc caatatttca
    38401 aaaactcata gacagcttct tttttattat tatctgtact tgccggaatg gcatatccta
    38461 cgccatctaa aatagctgtt tttttgtctg aatcactccc ttgtggtaag gtagtcacat
    38521 tataatcaat ctcgtttgct ttcaatccgt tatttaacca tggcccattg atttcaatgg
    38581 ctagttgacc agcattcatt aaattgtcca tttcggcgcc agaaatcgat tctggaccag
    38641 tcttgtctac atggatcatt ttttggatcg cttttaacgt tttaaggttc tcaggagacg
    38701 caaattcaga cttagtataa tcatcgttca ctaatttacc gccatttgct aacatccagt
    38761 tatataacgg tgcagttcca tccttattga agacaaaacc tgatacattt ttactactat
    38821 cagttaattt tggtgccatt tctgtcaatt cttcccaagt agtaggtggt tgttcagtat
    38881 ctaatccagc ttgtttaaac aaatcattat tccaatacat atacatgcct tgtacctgca
    38941 tggggacaaa atactgttta ccttctactt cacctaattc aatagccgtt tctttaaaat
    39001 cagatttatc tactccatca tattcccaga aatcgtctaa tggttgtacc gctccgtttt
    39061 ctacatactg tgcaaaatct ggataattta atgcaataaa gtcaggtgct tttttagaag
    39121 aaatcgctgt aggaagtttt tcattcaaat tggcccaagt catgatatcc atttcaattt
    39181 tgatgttttt atcatttgtt ttattaaatt cattaacgat ttcttgcaat atttcaccat
    39241 cagatgcagt aaacccattc caaaaagtca aagtcacttc tttatcagaa tcagattctg
    39301 ctgaacttcc gccaccgcaa gctcctaaaa tacctgttgc agctaccaac aaagagccaa
    39361 ttaaccattt tttacctttc attttttgtt cctccttcat ctgataaacg acatctgacc
    39421 acattccaag atagcggttt caataaacaa ttgattttcc catcttcagc ttttacattt
    39481 gttgaatgac ttggttttac ttgttcactt ttaggaagat tagttgcttt tgtatcaaat
    39541 ccactcattt cagaaaaatc aatcactccc tctaatgata attcagacaa gtgaacagtt
    39601 agattcattt cttcttctcc acggttaact gcaaatatga caagttcttt gtcatttatt
    39661 acggctatac tttctaaata cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa
    39721 cgaatttgtg ggcttaacac ctctccttgt ccatacacag cagcttgcat aaatggataa
    39781 tagatcgtct gtctccaagc ttctccgttt gtttctgtcc tgattggtgc aataacgttt
    39841 accaattgag caagacaagc aatctttact ctatctgcat gtttcaataa agtgattaat
    39901 agacagccta atagtaatgc atcttcaaaa ttatagttat cttctagtaa aggaggagca
    39961 atctgccaag gcgctgcttg ctcatcttgt tccgctgaat gataccaaat attccattca
    40021 tcaaaggaca ggttgatctg cttcttacta tgtttcttcg ccttgactgt atcgcacatc
    40081 gctgtcactt cagaaatgaa ccgatccatg tctaaggaac gtgcaagata atgagaaaga
    40141 tcattttttt ggttgccata atattgatgc agagacaaat aatctatttg atcgtacgct
    40201 tgatcaagaa ctgttaactc ccaatcacca aatgtaggca tttgccgatt cgaacttccg
    40261 cataaaacca cctcaattga atcatctacg agtttcatcg ccttagccgt ttcctctgct
    40321 aaccgtccat attcttccgc tgttttatgt ccaatctgcc aaggaccgtc catttcattc
    40381 cctaaacacc atgtcttaat tccaaaaggg ctctcttgac cattcgaacg ccttaaatca
    40441 ctccaataag ttcctttagg aaaattacaa tattcgacta aattacgagc tgcatctact
    40501 ccacgagtac ccagattgac agccatattg atttcggccc ccacatgctg acaccagtta
    40561 taaaattcat gaatcccaac ttcattcgtt tcaattgtcc gccaagctaa atccaacctt
    40621 ctaggtctat ttgctatggg cccaatactg tcttcccaac gaaatcctga cacaaaattt
    40681 ccaccaggat atctaatcat gggaatgttc aattctttta ccaagtgaat gacgtcttgg
    40741 cgaaatccac ttttattagc tgatggatga tctggctgat aaatcccttc atatactgct
    40801 cgtcccatat gttcaataaa tgaactgtac aaccgcggat caactttgcc tacacgcttg
    40861 acactactaa tcaacattgt ggcttccata actgacctcc tttaatttat acatgaataa
    40921 tacacctcgt tgaaagcgtt gtcaataata taaatgttaa acttacttat tttttatcga
    40981 ttttaattaa ccaaaatatt aataatgatt aaaacaaata atttaatata acttttatta
    41041 acaaatatgt taatatataa gaaaaacctt ttaagaggtg atatatatgc aactacaaat
    41101 aaacgaagaa tccttaccgg tatatgaagc attagctagt aaaacaagga tcaagatcat
    41161 ccagctactt tccaaaaaaa agatgaatgt caaagatttg gcaaaagaac ttggagtgag
    41221 cagtgctatc acgacgatgc acgtgaaaaa gctagaggaa gcaaacatca tcaaaacaga
    41281 aaaagtagga caacaaaaaa tttcgagcct tcgtgtcgat aaaatcgata ttagttttcc
    41341 agaaaaaata ttcaatgctt ttgatacgaa agaaacatcg atacctattg gtcactatac
    41401 aaattatgca attgaaccaa catgcggttt agctacgatc catgatttta tcgggaaagt
    41461 ggatgaacca cgctatttta tggatcctcg aaggatggat gcccgcattc tctggtttac
    41521 cagcggattt gtcgagtatc aggcacctaa ttttttaaat acagaggata cattggagat
    41581 gttggaagtt tctgtggaaa tttcttctga atttcctttt tcaaatgaca attggccttc
    41641 agatatcact ttcagcctaa atggtgtgga gctaggaaca tggacgagcc ctggagattt
    41701 tgctgatatc cgcggtaaat atacgcctga ttggtatcca gataatctta atcaatacgg
    41761 cttgctgaag acgatccgaa ttaccaaaca cttgacgaat atgaatggag aacccttgtc
    41821 aaacatcacg atcaacgaca tccctaaaga gcaagacact tggcatttgc gaatcgaagt
    41881 gaaagacgat gccaaacatg tggggggctg tactttattt ggtaaaggat tcggtaatta
    41941 cgatcaggat atcaaattaa aagtttacta cagctgatat ttttcttttc tattttataa
    42001 aacaaaaaca gccaatccct atgaagggac ggctgttttt gctttattgt tatttccaga
    42061 aatcatccaa aacggaaatt ggcaaatgtc gtttatgttg tgtcttgttc caccatgctt
    42121 cgattttttc ttgtgcttga gcgggaactt cttttccttc tagataatca tcaatttcat
    42181 tatatgtgac acctagtgcg acttcatctg caatcattgg tttcccatct tcaagatctg
    42241 cagtcggaat ttttgtgtat aatttttccg gtgcattcaa tgcttgaagc aattgtttcc
    42301 cttgtcgttt gtccaaacgg aataatggca aaatgtcggc tccaccatca ccaaatttcg
    42361 tgaagaatcc agtaatgttt tctgcagcat gatctgtccc taatacggct cctgctcttt
    42421 ctccagctac ggcatattgc gtaatcatac gttgacgtgc tttgatattt cctttgttaa
    42481 aatcgctgat tcccacacct gcttcgctta atacttgtac ttgtgcatca acagctggtt
    42541 tgatatttac tcgcagacta acatcgggct ggataaagtc aagagcagct tttgcatctt
    42601 cttcatctgc ttgttcacca taaggaagac gaacagcgat aaattgatag ttttcatctt
    42661 ttgtttcttc acgcatttct tccatcgtta gttgcgccaa acggcctgct aaagtagaat
    42721 cttggccgcc gctgatacct agtacaaaag ttttaagaaa cggatatttt ttcaaatatg
    42781 ctttcataaa gtcaatactt ttacgtattt cttcttttgg atcaattgtt ggtttgacac
    42841 ctaattcatt gataatttct gtctgcaggc tcatttattc tcctcctatg cttctgtatg
    42901 aagttctgct acgctttttt tcattcgttc catgatagcc attttatggt tccagcattc
    42961 agtagacaga tcgactgggt atttttgcgg attcaagtct cgtttatact cttcccatag
    43021 tgaatctaga ttttcttttg tatattcttt gatttcgttt agtgcaggta attcgtaaac
    43081 tctttttcct tcgataaaga tatcttgtaa aactgggcga gctgtaaagt ctcgaacagt
    43141 tttattgata aacgtatgca ctggatggaa catgaagata gcttcttctt ctcgagggtc
    43201 ttcatcccat aatgtcacgt agtctccttc agatttccca tcaaaatttc gagtgatgcg
    43261 ccacacttgt tttttccctg gagtagttac tttttctgca ttgcttgata acttgatcgt
    43321 atccatcatt tttccttcat catcttcgat cgatactagc ttgaatactg caccaagggc
    43381 tggctgatca taggctgtga tcaattttgt cccaacaccc catacatcaa ttttagcttt
    43441 ttgcatcttt aaattcaaaa tcgttgcttc atccaagtca ttcgaagcat agatttttgc
    43501 ttccgtaaat ccggctgcat ctaattgttc ccgaacacgc ttagatatat aggccatatc
    43561 tccactgtcg atgcgtacgc cttggaagtt gattttgtca cctaattctt tcgccacgcg
    43621 aatcgcactt ggcacacctg atttcaacgt atcataagtg tcgactaaga acacgcagtc
    43681 tttatgggtt ttagcgtagg ccataaatgc ttcataatca tttccataag attggactaa
    43741 cgaatgagca tgggtcccgc tagctggtat cccaaatatc ttcccagcac gtacattgct
    43801 tgttgcatct gctccgccga tatatgccgc tctagttccc cagactgctg catccagttc
    43861 ttgtgcccgt ctagttccaa attccaatag tggatcttct ccgatgacag atttgatacg
    43921 agctgctttt gtcgcgatca acgtttggaa attgaccata ttcaacagtg ctgtttctac
    43981 taattggcag tgcgctaaag gcccttccac ttggatcaat ggttcatttg caaaaactag
    44041 ttctccttca cgagcagaac gaactgttgc tttaaattca aaattttgta aatactccaa
    44101 aaaaccttca ggatagactt ctaatccacg caagtaagca atatcggaat cattgaacgt
    44161 cagattctct aaataattga ctaaccgttc caatcctgca aaaaccgcgt atccgtggtt
    44221 aaaaggcatt tctcgaaagt aacattcaaa aacagcatgt aaatcagctc gacctaattc
    44281 ccagtatgtc tgcatcatat tgatttgata tagatccgta tgcagcgtca aactgtcgtc
    44341 tgggtaagta ttattcatct tgtattactc ctcttggcaa gttttcattt cctacccatt
    44401 ataacaaaaa aaattaatag gaaaagaaat cttccatttt ttctgaaaaa gcacatgttt
    44461 tacggatatt tcttgctaaa aatgaactcc atctgctaaa actatccttt cttattggta
    44521 tttaacctaa acgagaaaga atacttattt tattcaatgt atccgagaca cgaagaaatt
    44581 agcgttttct atttttctta cttgataaaa taagtagatt ttcctactac aatcgtagcc
    44641 gttaagatac cgatgattga acaaatataa ccaaatgtct ctacaacata atcaatggca
    44701 aaagtttttg aagcaataat acgtctttcc ttttttgctt cttatcccca aagaaacgga
    44761 tataacttat tatgtattga atttttttgg aaaggcgaat tatgacaaga tataccttgc
    44821 gttgaaaaaa ctcgcggaaa aaatatcaca cgtttttaaa aagatacaaa gtaagaaagc
    44881 agctgtcaat agaatggaaa caaaaatggc gagacatcaa tcagtctcgc cttgttttt
//
LOCUS       DARBDV010000011        44863 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000011 DARBDV010000000
VERSION     DARBDV010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.011"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            212..604
                     /locus_tag="SCM56_000619"
     CDS             212..604
                     /locus_tag="SCM56_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEG1109443.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="SCM56_000620"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="SCM56_000620"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEG1109444.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="SCM56_000621"
     CDS             3165..4775
                     /locus_tag="SCM56_000621"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEG1109445.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="SCM56_000622"
     CDS             5067..5936
                     /locus_tag="SCM56_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEG1109446.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="SCM56_000623"
     CDS             6101..7372
                     /locus_tag="SCM56_000623"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEG1109447.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="SCM56_000624"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="SCM56_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEG1109448.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="SCM56_000625"
     CDS             8763..9026
                     /locus_tag="SCM56_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEG1109449.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="SCM56_000626"
     CDS             9471..9920
                     /locus_tag="SCM56_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1109450.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="SCM56_000627"
     CDS             9977..11395
                     /locus_tag="SCM56_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1109451.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="SCM56_000628"
     CDS             11395..12255
                     /locus_tag="SCM56_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HEG1109452.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="SCM56_000629"
     CDS             12429..13202
                     /locus_tag="SCM56_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEG1109453.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="SCM56_000630"
     CDS             13349..13714
                     /locus_tag="SCM56_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HEG1109454.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="SCM56_000631"
     CDS             complement(13862..15070)
                     /locus_tag="SCM56_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1109455.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="SCM56_000632"
     CDS             complement(15089..15367)
                     /locus_tag="SCM56_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109456.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15521..16216
                     /locus_tag="SCM56_000633"
     CDS             15521..16216
                     /locus_tag="SCM56_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEG1109457.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16658..17455)
                     /locus_tag="SCM56_000634"
     CDS             complement(16658..17455)
                     /locus_tag="SCM56_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEG1109458.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="SCM56_000635"
     CDS             complement(17452..18636)
                     /locus_tag="SCM56_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEG1109459.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="SCM56_000636"
     CDS             complement(18661..19908)
                     /locus_tag="SCM56_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEG1109460.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="SCM56_000637"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="SCM56_000637"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEG1109461.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="SCM56_000638"
     CDS             21888..22202
                     /locus_tag="SCM56_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEG1109462.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="SCM56_000639"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="SCM56_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEG1109463.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="SCM56_000640"
     CDS             22887..23531
                     /locus_tag="SCM56_000640"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEG1109464.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="SCM56_000641"
     CDS             23546..23875
                     /locus_tag="SCM56_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEG1109465.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="SCM56_000642"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="SCM56_000642"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEG1109466.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="SCM56_000643"
     CDS             24863..25687
                     /locus_tag="SCM56_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HEG1109467.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="SCM56_000644"
     CDS             25680..26027
                     /locus_tag="SCM56_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEG1109468.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="SCM56_000645"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="SCM56_000645"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEG1109469.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="SCM56_000646"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="SCM56_000646"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEG1109470.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="SCM56_000647"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="SCM56_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEG1109471.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="SCM56_000648"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="SCM56_000648"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEG1109472.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31657..32451
                     /locus_tag="SCM56_000649"
     CDS             31657..32451
                     /locus_tag="SCM56_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1109473.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="SCM56_000650"
     CDS             32441..34483
                     /locus_tag="SCM56_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1109474.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="SCM56_000651"
     CDS             34764..34913
                     /locus_tag="SCM56_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEG1109475.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="SCM56_000652"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="SCM56_000652"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEG1109476.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="SCM56_000653"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="SCM56_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEG1109477.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="SCM56_000654"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="SCM56_000654"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEG1109478.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="SCM56_000655"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="SCM56_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEG1109479.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="SCM56_000656"
     CDS             39330..40412
                     /locus_tag="SCM56_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEG1109480.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="SCM56_000657"
     CDS             40412..41215
                     /locus_tag="SCM56_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEG1109481.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="SCM56_000658"
     CDS             41285..42025
                     /locus_tag="SCM56_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEG1109482.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="SCM56_000659"
     CDS             42036..43040
                     /locus_tag="SCM56_000659"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEG1109483.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..44782)
                     /locus_tag="SCM56_000660"
     CDS             complement(43085..44782)
                     /locus_tag="SCM56_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109484.1"
                     /translation="MKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLE
                     KPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTL
                     TVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMY
                     KQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYY
                     WSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLAC
                     IGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISY
                     NLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLM
                     HTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLP
                     EELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYD
                     TNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT        14423 a         7827 c         9342 g        13271 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaactttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttttta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggcatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagaacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgttaaa aataacaatg ataatataat gat
//
LOCUS       DARBDV010000012        40009 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000012 DARBDV010000000
VERSION     DARBDV010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.012"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..290)
                     /locus_tag="SCM56_000661"
     CDS             complement(<1..290)
                     /locus_tag="SCM56_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HEG1109485.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
     gene            complement(283..624)
                     /locus_tag="SCM56_000662"
     CDS             complement(283..624)
                     /locus_tag="SCM56_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109486.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELSCDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYNRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            complement(798..1523)
                     /locus_tag="SCM56_000663"
     CDS             complement(798..1523)
                     /locus_tag="SCM56_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HEG1109487.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNM
                     KVMEVLGGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLP
                     ETAIRMEIDQLNRQQRLFGDENE"
     gene            1576..2052
                     /locus_tag="SCM56_000664"
     CDS             1576..2052
                     /locus_tag="SCM56_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109488.1"
                     /translation="MRKKLNYILTATLTIYIILFITLSFLSSIKKIPFITLNKNSILK
                     EFNNTDYIAFLKLFSVCVLISCVIIFIVAKTCWTMLNKNQRDVENIAIFLIKITSSIS
                     IIIPMTVTFSKEQFNVAATYLSFIALFSFVLPKRNKSNFDNKELDEVRNSSPKEQK"
     gene            complement(2058..2240)
                     /locus_tag="SCM56_000665"
     CDS             complement(2058..2240)
                     /locus_tag="SCM56_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109489.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEHRAKKASREGLTKI"
     gene            complement(2240..2539)
                     /locus_tag="SCM56_000666"
     CDS             complement(2240..2539)
                     /locus_tag="SCM56_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109490.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            complement(2644..2847)
                     /locus_tag="SCM56_000667"
     CDS             complement(2644..2847)
                     /locus_tag="SCM56_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1109491.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(2862..3608)
                     /locus_tag="SCM56_000668"
     CDS             complement(2862..3608)
                     /locus_tag="SCM56_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HEG1109492.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            3686..3994
                     /locus_tag="SCM56_000669"
     CDS             3686..3994
                     /locus_tag="SCM56_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109493.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            complement(3965..4222)
                     /locus_tag="SCM56_000670"
     CDS             complement(3965..4222)
                     /locus_tag="SCM56_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HEG1109494.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(4520..4660)
                     /locus_tag="SCM56_000671"
     CDS             complement(4520..4660)
                     /locus_tag="SCM56_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109495.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            4960..5280
                     /locus_tag="SCM56_000672"
     CDS             4960..5280
                     /locus_tag="SCM56_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1109496.1"
                     /translation="MNTYEIIKELAKEKGLSIRQLEMNFGYSNGYLGSWKRQTPNSIE
                     LARLADYFGVSVDYLLGREERETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            5298..5729
                     /locus_tag="SCM56_000673"
     CDS             5298..5729
                     /locus_tag="SCM56_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEG1109497.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            5856..6440
                     /locus_tag="SCM56_000674"
     CDS             5856..6440
                     /locus_tag="SCM56_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HEG1109498.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            6542..7690
                     /locus_tag="SCM56_000675"
     CDS             6542..7690
                     /locus_tag="SCM56_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1109499.1"
                     /translation="MLKVWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWK
                     KAQKILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHI
                     SDDTIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPAL
                     AVKIHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRY
                     GELLALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIES
                     VIADNILAGRPTDPDQYIFISTSGTPLALHSFNAILHKVEEELELEKSLSSHIFRHSH
                     VSLLSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(7779..9404)
                     /gene="groL"
                     /locus_tag="SCM56_000676"
     CDS             complement(7779..9404)
                     /gene="groL"
                     /locus_tag="SCM56_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEG1109500.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(9455..9739)
                     /gene="groES"
                     /locus_tag="SCM56_000677"
     CDS             complement(9455..9739)
                     /gene="groES"
                     /locus_tag="SCM56_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEG1109501.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            9964..10626
                     /locus_tag="SCM56_000678"
     CDS             9964..10626
                     /locus_tag="SCM56_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEG1109502.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            complement(10702..11994)
                     /locus_tag="SCM56_000679"
     CDS             complement(10702..11994)
                     /locus_tag="SCM56_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1109503.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            12164..12793
                     /locus_tag="SCM56_000680"
     CDS             12164..12793
                     /locus_tag="SCM56_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEG1109504.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            complement(12896..13705)
                     /locus_tag="SCM56_000681"
     CDS             complement(12896..13705)
                     /locus_tag="SCM56_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEG1109505.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(13760..14629)
                     /locus_tag="SCM56_000682"
     CDS             complement(13760..14629)
                     /locus_tag="SCM56_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HEG1109506.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            complement(14630..15946)
                     /locus_tag="SCM56_000683"
     CDS             complement(14630..15946)
                     /locus_tag="SCM56_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109507.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            complement(15943..17778)
                     /gene="walK"
                     /locus_tag="SCM56_000684"
     CDS             complement(15943..17778)
                     /gene="walK"
                     /locus_tag="SCM56_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HEG1109508.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            complement(17783..18487)
                     /locus_tag="SCM56_000685"
     CDS             complement(17783..18487)
                     /locus_tag="SCM56_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1109509.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            complement(18677..19537)
                     /locus_tag="SCM56_000686"
     CDS             complement(18677..19537)
                     /locus_tag="SCM56_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEG1109510.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            complement(19524..20093)
                     /locus_tag="SCM56_000687"
     CDS             complement(19524..20093)
                     /locus_tag="SCM56_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEG1109511.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            complement(20667..21836)
                     /locus_tag="SCM56_000688"
     CDS             complement(20667..21836)
                     /locus_tag="SCM56_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1109512.1"
                     /translation="MKLRANIIEKRGTLQVVVSYKDSNGKIRQKWKDTKLKKKGNKKR
                     AEKIRDEFLLELESQLAMECECANPNLLFYDYLLNYLEVSKKQVAFNTYVGYKHYVHN
                     RIYKFFYPKKIKLNRLKPYHLQEFYQYMLNDNCTANTVIHYHAFIRKALQEAVITELI
                     STNVADKVRKPKKKQFVSQVYNQQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGL
                     KWSAIDFLNKKIMINHVIIENPENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAK
                     WQQDNRILLGDDYILEDNEYVFTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRH
                     SNASIMLDSGQNMKSIQEWLGHASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            complement(21880..22110)
                     /locus_tag="SCM56_000689"
     CDS             complement(21880..22110)
                     /locus_tag="SCM56_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1109513.1"
                     /translation="MDFQDYEKIFESYPSILKITDLTKMLNIGKNKAYNLLKDRSIEN
                     IKIGARYKIPKKAVIEYILKIQDEGKNHAIIK"
     gene            22186..22258
                     /locus_tag="SCM56_000690"
     tRNA            22186..22258
                     /locus_tag="SCM56_000690"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22219..22221,aa:Lys,seq:ctt)
     gene            22420..22641
                     /locus_tag="SCM56_000691"
     CDS             22420..22641
                     /locus_tag="SCM56_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109514.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
     gene            complement(22965..23210)
                     /locus_tag="SCM56_000692"
     CDS             complement(22965..23210)
                     /locus_tag="SCM56_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1109515.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(23204..23605)
                     /locus_tag="SCM56_000693"
     CDS             complement(23204..23605)
                     /locus_tag="SCM56_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEG1109516.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(23777..25585)
                     /gene="uidA"
                     /locus_tag="SCM56_000694"
     CDS             complement(23777..25585)
                     /gene="uidA"
                     /locus_tag="SCM56_000694"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HEG1109517.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNG
                     KLINQHKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKK
                     ENLVNFDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILI
                     AIYDEEAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGI
                     RSIQVSNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKM
                     AIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
     gene            complement(25613..27379)
                     /locus_tag="SCM56_000695"
     CDS             complement(25613..27379)
                     /locus_tag="SCM56_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HEG1109518.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGISPIDAYCGLKDTFI"
     gene            complement(27392..28186)
                     /locus_tag="SCM56_000696"
     CDS             complement(27392..28186)
                     /locus_tag="SCM56_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEG1109519.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDILFKCLSGEYIDIEQKYIDTFWKNLTNK"
     gene            complement(28197..29573)
                     /locus_tag="SCM56_000697"
     CDS             complement(28197..29573)
                     /locus_tag="SCM56_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1109520.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
     gene            complement(29702..30349)
                     /locus_tag="SCM56_000698"
     CDS             complement(29702..30349)
                     /locus_tag="SCM56_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEG1109521.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            complement(30433..31860)
                     /gene="uxaC"
                     /locus_tag="SCM56_000699"
     CDS             complement(30433..31860)
                     /gene="uxaC"
                     /locus_tag="SCM56_000699"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEG1109522.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            complement(31934..33013)
                     /gene="uxuA"
                     /locus_tag="SCM56_000700"
     CDS             complement(31934..33013)
                     /gene="uxuA"
                     /locus_tag="SCM56_000700"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEG1109523.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(33016..34647)
                     /locus_tag="SCM56_000701"
     CDS             complement(33016..34647)
                     /locus_tag="SCM56_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEG1109524.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            34964..35635
                     /locus_tag="SCM56_000702"
     CDS             34964..35635
                     /locus_tag="SCM56_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1109525.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQ
                     KKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERF
                     RWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVVLTTHPK
                     YFKNI"
     gene            complement(36471..37427)
                     /locus_tag="SCM56_000703"
     CDS             complement(36471..37427)
                     /locus_tag="SCM56_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109526.1"
                     /translation="MLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVP
                     LSEQPDLKRMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSC
                     ERSRLKEQWSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKG
                     NLLEYFQIRRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLE
                     NAGYREGQLFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQS
                     SLPKCQLVYTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(37536..37781)
                     /locus_tag="SCM56_000704"
                     /pseudo
     CDS             complement(37536..37781)
                     /locus_tag="SCM56_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(37926..38348)
                     /locus_tag="SCM56_000705"
     CDS             complement(37926..38348)
                     /locus_tag="SCM56_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109527.1"
                     /translation="MVKEEVLCPHQDFLYLIPANQETTQHYFTFLEKQNELVEALMES
                     QETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHNYELIPEHWQLLGSSKQQ
                     GKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            complement(38528..38899)
                     /locus_tag="SCM56_000706"
     CDS             complement(38528..38899)
                     /locus_tag="SCM56_000706"
                     /inference="COORDINATES: protein motif:HMM:NF016724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEG1109528.1"
                     /translation="MWLSDEKIQWRSYQKRILTNFATYLNNNSVHLVAPPGAGKTFLG
                     IEMIRRLDCKTLILVPSLILKKQWLTTIEEYFLVNPADSCYLSGAIEHPSHITVETYQ
                     TIYNRLKEEPTYGDQLELLVF"
     gene            complement(39233..39496)
                     /locus_tag="SCM56_000707"
     CDS             complement(39233..39496)
                     /locus_tag="SCM56_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109529.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHYIRNRWSSIPNFNYKK"
BASE COUNT        12151 a         7949 c         6115 g        13794 t
ORIGIN      
        1 tctgtttccg tatgtccttt aaccgttact gagacagata cataagtccc tgtttcatcc
       61 ataagaaaag gtttatattc ctcaacaaga aagtcttgat gagttccaga aacaaccctg
      121 tagtgtttat actcattaat agttaccgtt gcctgtggat cattcttttt cataatctcc
      181 cacgcgtgag cccaagataa ataatcaaaa tttccttttt tcttgagaat tttatttaac
      241 ttacgactaa aaagtttttc aaagttcgtt gtccctttga tttcactcat caaattctgc
      301 ctccatttca gcaatgtatt tcttacctga tccgtaataa gagatatcaa tcaagttatc
      361 tctgttgtac tcttctagcg catcaatcaa gccatcttcg atgacataga tgtattcagg
      421 tttatttgaa tgttttgata gatggataag gtaaacatga tcccaaatac tcacaaaatt
      481 tcccaagtca tcttgatcac atgatagttc ttcgttcgtc aaaagatttc gtctgatttt
      541 tcgattattt gtttccttga tattcgactt gccccattca gaatcagtca aatattgatc
      601 taaagtggaa agttctttat tcatatgcta aaattctcct tagatatgtt ttgtgactct
      661 atgcttgccg gctgagtcac ttttttattt gttgccatgc tttttgcttt tcgatatgtt
      721 gttggcttag gatgtttggt ttattgtgtc tccaccagcg attagcaatt accgtcccta
      781 ttcttagcgc ttcagctcta ttcattttca tcaccgaaaa gtctttgttg tctgttcagt
      841 tgatcgattt ccatgcggat cgcagtttct ggtaaccaca tttcaataaa tgaaacagca
      901 tcatcgaatc tcttacgagg taactcgcca tatcttggga ttgaaaaggt acgtttaaat
      961 tcagaccaaa attttgagaa tacttttttg ctgatttctt cataagctcg gctttctttt
     1021 ccccctagaa cttccataac tttcatattt cctttttgct taatttcaaa ctcttgttgt
     1081 ccgctaattc gcatagtatc tttaagcatg gaaacatctt ttttaacatc tttcatttct
     1141 tctaattgat agatcatcat gtcttcaatt gtttgaggaa cagtattctt ccgaataaca
     1201 tcttccattt cgttgaaagc ttcaataaat tttagtttga aatttattgc tttacttcca
     1261 gtgaatccca tagctagcaa ggaaaatccg tctctattca tgaaataaac tctccgactt
     1321 cttccgtatg agtctggctc gttcccttcc acaaacatct gtccaaaatt ggacccatct
     1381 tcaacattgg ccgaattttc gaccgatctt tttattgatt caattgcttc tagtacatgc
     1441 ttatgtttct ttttgaaact ttctgccact tgtaagctcg tagttacagc ttctttattt
     1501 ttcaaaatta ctaattcttg cattatttct tctctccttt ttggtataat tttcgttaga
     1561 aagtgaggtg attctatgag gaaaaagctc aattacattc ttactgcgac tttaaccata
     1621 tatataattc tgtttattac cttatctttc ctgtcttcaa ttaaaaaaat tccttttatt
     1681 acattgaata aaaatagtat tttgaaggag ttcaataaca ctgactacat cgcatttcta
     1741 aaactttttt cagtctgtgt gctaatatca tgtgtaatta tttttattgt tgctaaaaca
     1801 tgttggacaa tgttaaataa aaaccaacgc gatgttgaaa atattgctat atttttaatt
     1861 aagattactt cctcaatatc aattatcatt ccgatgactg taactttttc aaaagaacag
     1921 tttaatgtag cggctactta tctttcattt atagcgctat tttcttttgt tcttcctaaa
     1981 agaaataaga gcaactttga caacaaggag ctggatgaag tacgcaatag cagcccaaag
     2041 gaacaaaaat aaaaaaatca tattttagtc agtccctccc gactggcttt tttcgctctg
     2101 tgctcagctt catcaagccc cataaaaatc caaaccatgt aaacgattgt ccctatcaac
     2161 gcttgtctgc ttccccagag gcctaaagcg tagatgatta gcggtgcgct gaatactagt
     2221 gctctgttga atttgcccat taacttgcct ccttcaaaac atctgaaggc gataaacttt
     2281 ttacacgata tttgtttgcg tctttccact ttaaaaacca aataaaagta tggtaatgaa
     2341 taaatgtggt tgagtgacct ggacgaagta ttccttctgc aaattcagga attgtttcca
     2401 tttccttgca gtattcaact aaagtagttt ttgacatgcc gtggaacttc tccaagatta
     2461 aactttgtcg gtaccagtcg tctggtttta ctgccttctc agcagcttct atcagctctg
     2521 aaagtgttgg ttttttcact tattatccct cctaaaaatt atcgttccat actcgatgtt
     2581 taacatttct tgaagctctg attttgtaag aatgatttta tcttccaacg actttacctc
     2641 cctctacaat tcgtacatag tgataatcga atctataatt ctgtttgctt ctgcagaagt
     2701 ctttttaccg tttaaaatta aagataagta gcttttgcta attccaaatc tttcagcaag
     2761 catggtgtaa gttaagaact ttgaactttc gacatattct ttgatttttt ctctatctcg
     2821 ttgagtgatt tctgcaatgt cagtcatact aaaactcctt tctaaccaat ttcctctaaa
     2881 tccatttgag ggtaatagcc ctctgcgatt aataagttgt aaataaacac tcgccctttc
     2941 tgtgtccatt tggtattcat tacaacttta gtgccgccat cggctttcgg tatctcactt
     3001 gtgtgcgatt ttgtgtatcc ttggttcata tgttttcggt aaagtatcca ttgtccgcta
     3061 actttatgct gaacacctaa ctcgtttagc aatttgttta gagcaattgc cgacattccg
     3121 taatcagctg caatttgaga agtagctacc gtatctgtcg atgaaagaat cgtatctaag
     3181 tagctaatct ttggttcgta ctcggcaatt tgttgttcga gtaattggtt cttttcttct
     3241 aaatccgctg ctaaacgcaa tgcttgtgca aaactttgag gtacgtttga atagctacct
     3301 gtttttctaa ttgttggaag gacttcactc gttacccaac gtttaaattt tttggcagag
     3361 ggaagttttg attttaagat taaactgtaa aggcctgact cgttaatcaa aacaactgct
     3421 gttccattga cggtgaacga atcgttctgc gtcttatcct ccaagtccac atgatctcga
     3481 atagcttttt gcggattcga atatcctaga acgtctgcta catcttttcc tacaaaatat
     3541 ggttcatcat ttactaaaat agttcgaact tcgttttgct cgaaattaaa aatttgtggt
     3601 gtattcatat ttctcattcc tttctttggt ataatttcct tatcagtgtg acaggctgaa
     3661 ataattgtta aggaggtgaa aatttatgaa accagaattt gtaattacta tgcttgatgg
     3721 agaaaaaatc aaaatatttg ccgatacttt ggtcgttgga tacgatgatg cgtcaaatat
     3781 cgaagaaaaa gatggaatgt tctattcttc tcaaatttac gtgaatacct tgcaaggtga
     3841 tattggggta tctactcttg caacatcaag accgcttgta ggtcttatcg gctttttgtc
     3901 aaaagcaaca ttcttttctc tcggcgatga tatagattcg gatgaaactg tcatctataa
     3961 aacatcagct gttaaaagta tcggtttcaa atagtaattc ttgctcgttg atagcggaag
     4021 ccaacttttc ttccaacaca tcggcgagct ttctttttgt tggattcatt tcaaagttga
     4081 tagtcttgag aagcattttt gcatccttaa ctttttgtcc ttgcaattcc ttgatgattt
     4141 tatcggcttg gctatcaatt ccatgtgtta gatcatctat cattttttgt accttgattg
     4201 tttggctcat ttccttgttc attgtttagc ctcctttaat aaatgatttt ttgtattttt
     4261 taatttccat aaattcctca aattgctctt tggtattatt accacgacca tacatattgt
     4321 gaaattcttt atggcaactg acacaaagtg taacgccgtt tgaaatcgct gcggatttag
     4381 ttgttgcatg gctaaatcac gaatcacaag tatctactaa aggcgaatca atttcgccaa
     4441 aagaaattgt tcaagcttat ttagatattc attatgctgt tatctatggt cagcttccag
     4501 aagaccgtaa aaacgatgac taaacgtgag ctaaaatctc tgctatggct gcaaccatgg
     4561 cagagtcttc tttatctacc gcgtgttcca tcgccacttt tgcttgtttc agtatgtcta
     4621 atttcaaatc ttcgattttt gccgagactg ttttttccat attcttctct cctttctttt
     4681 atattcgtaa acaaatttaa caacttttta aaaaactgcg ttgactttat tagattaata
     4741 atctatacta aatgcatagt taaataagac atataaactt tgatttaaaa agctttcttg
     4801 gcggttggca tttattaatc aatagtattt tttgttgtct ttttagttgt taaacttgtt
     4861 tacaaaacat agtatagatt aataatgtat ataagtcaac tatttttaca ttatttatct
     4921 aaactttttt tgtaagcatt cagaaaggtt gatttatcaa tgaatactta cgaaataata
     4981 aaggagttag caaaagaaaa aggattatcc attagacagc tcgaaatgaa tttcgggtat
     5041 tcgaatggat atttaggaag ttggaaaaga caaactccta attctattga gttagctcgg
     5101 ttagctgatt actttggagt ctctgtagac taccttctag gtagagaaga aagagaaacc
     5161 cctaaacatg tggatttatc agaaaacgat actgtttttt cttttgatgg aaaagaaata
     5221 tctaaggaga caatgcgtaa agcgattgca attgctaaag ctttagagga aaatgaatag
     5281 ttggagtgat gggttgtatg tatttgaaat tgaaagaaat gttgagcgaa tacaatttga
     5341 aagtagttta catggaaatg aaagagcctg gcttctatta tccgaagcct agaatcattt
     5401 ttttgaatga aaatctatat ggcgaaactg cagaagcctt tcatttatct catgaacttg
     5461 cgcatttcac cgcttctcat tttgaatatt cagttttgta cgatacttct acaacttttc
     5521 actcaaaatt tgaaacagaa gcagataaaa tagctattct aattttatta aatatctata
     5581 ttgagaacga actgactgat gaatctcagt ttaatctcga aaaatttatg gagtattatt
     5641 ctattcagaa taagctcaga tacacttgtt atgctgtctg ccaatgctat tttaagaaaa
     5701 aatattctta cgcaaggcaa tatgtatgaa tacatcaaga attattaaaa aaaccgattt
     5761 cagcaaaaca ttaaaaaagc ccgtgctggc acacggactc atacctcatt tctgagatcg
     5821 caaatatatt ataacaagaa gtgaggaata tttaaatggc aaaaaaagtt atgggtcaag
     5881 acggaaaaat gtataaagtt agtaaacctt tttacaaaaa agtatggttt tgggtattag
     5941 cagtaatttt aattataatt attggttctg ctttaaatgg tggatcagat agtaataaag
     6001 caagtgataa tggtggcgaa aaagtaacta aatcttcaac ctctgcttca tcttctaaag
     6061 aagaaaaaag cgataccttc tataagattg gtgacactgt aaaagttggt gatgctgaat
     6121 atacactaaa tagtgtagaa ctaactgatg aaagaaatca atttgaagaa aaccaacctg
     6181 cacaagtagt aaaaattact tataccgtaa aaaatgatgg cgattcagat atccctgtag
     6241 gtacagatgt ggaagtatat ggaccagatg acaaaaaatc agagacatat gccaacgaaa
     6301 atacaatggg atctgttgct ccaggaaaac aaatggatgt aactgctcat ttcacgttaa
     6361 accaaaaggg agaaatagaa atccacttct ctcctttagt atcatttgaa aaagcagcta
     6421 tttttaaagc aactgtataa taaataaaaa acacgcccca ccgtccagag taagagcgtg
     6481 cttttagaaa acaaacctat ataggtctat ttgttatgcc tattatagca aatgatagga
     6541 gatgttaaaa gtgtgggttg aacagaccaa agatggccaa ttcaagttta ttgaaagata
     6601 tgtagatcct tatacagaaa aaacacgaaa aaaatctaca acacttacaa gtaattcacc
     6661 acaagcgtgg aaaaaagctc agaaaattct agataaaaaa attaaagaag cactcgaaga
     6721 ttacaataaa tcagatatca cttttggtga gctgtataaa gaatggtatg aatattataa
     6781 gcagcatgtt aaacgtacta gctatttgaa ggttccaatg atgatgaaac atgtttctaa
     6841 gcacataagc gatgatacga tcgttagaaa cattgatgag acactcatta ataagataat
     6901 tgaagatatg tatacgtttg gtgacctctc actgaactac acaaaacaaa caaaaacaac
     6961 tctatctgtt atgctaaact atgcaatcga tagaaaatac attcaaagaa accctgcgct
     7021 agcagttaaa atccatccta aaaaagtgga agaagaaaaa agaaggcttt ctatgaataa
     7081 aaaatatctg gagaaagaag aaattgatca aatactgaaa cagctatact ccaatcctcg
     7141 cagaaaacta cacggcataa tagctgagtt tctatattta actggtttga gatatgggga
     7201 attgctagct ttgcaaatga aggactatga ggatgggaaa atttccatta atgggacctt
     7261 agattacaca tctgtgaaaa tggataatgc tataaaaaca actccaaaaa atacttattc
     7321 gcaacgtgaa gtgcaattac ccaatcgtgc aaaagaattg attgaaagtg tgatagctga
     7381 caatattctt gcaggtagac ccacagatcc cgatcaatat atatttatat ctacaagtgg
     7441 cactccgctt gcgctgcact catttaacgc tatactccat aaagtagagg aagagttgga
     7501 attagaaaaa agtctatcct cacatatatt cagacatagc cacgtttcac tattatctga
     7561 attaggcgta cctcttaaag ccattatgga gcgtgtaggg cattctgatg caaacacaac
     7621 tctgtctatt tataaccatg taacaaaaag agccaaacaa caagtaattg ataaactaaa
     7681 tagcctttga tatttttgcc ccttctttgc cccttttgct taaaataaaa caaaaaaaga
     7741 agaaagaacc ctgttaaaat agggttcttt cttaattact acatcatacc gcccatcatt
     7801 gatggatcca ttgcaggagc tgctggcgct gctggttctg gtttatctgc aacgactgct
     7861 tctgtcgtta ataacaaggc agaaacggaa gcggcatttt gcaaggcaga acgtgttact
     7921 ttagttgggt ctacgatacc tgcttcaacc atgtttaccc attctccagt agcagcattg
     7981 aaaccagtgc ctagttccac gttcttcaat ttatcaacaa tcactgatcc ttcataacca
     8041 gcgttttcag cgatttgacg aattggttct tctaaagcac gaacaacgat cttgatacct
     8101 gttgccacgt ctccttctgc ttctacagca gaaactttgc tgataacgtt cactagagct
     8161 gtaccaccac ctgagaccat tccttcttct actgcggcac gtgtagcatt caatgcatct
     8221 tcaatgcgta attttaattc tttcaattct gtttcagtag ctgcaccaac tttaacaaca
     8281 gctacaccac ctgctaattt agctaagcgt tcttgtaatt tttcgcggtc aaaatcagaa
     8341 gttgtttcag caatttggtt tttgatcaat tgcacacgag cttcgattgc ttctttttct
     8401 ccagaacctt ctacgatcgt tgtattgtct ttatctacaa caactttaga agcatttcct
     8461 aagttttcga ttgttgcatc tttcaattct aaacctagat cgtcagtaat tactgtacca
     8521 cctgtcaaaa tagcgatatc ttcaagcatt gctttacgac gatcgccgaa accaggagct
     8581 tttacagcta ctacgttgaa tgttccacgg attttgttca atacaagagt tggcaatgct
     8641 tcaccatcta catcgtcagc aataatcaac aatggacgtg attgttgcaa gatttgttct
     8701 aacaatggca gaatatcttg gatgttagag atttttttgt ctgtgatcaa gatatatggg
     8761 ttttctagaa cagcttccat tttgtcgtta tctgttacca tatattgtga taagtaacca
     8821 cgatcaaact gcataccttc tacaacatct agttctgttt cgatcccttt tgattcttcg
     8881 attgtgatga caccgtcgtt tccaactttt tccattgcat cagcaatcaa atgtccaact
     8941 ttatcagaac ccgaagaaac agctgctact tgtgcaatgg cttcttttga atcaacaaca
     9001 gttgagatat tatgcaattc ttcgactgct gcttttgttg ccaattcgat cccgcgacga
     9061 atgcctagag gattagcacc tgcagttacg ttttttaaac cttcgcgaac gattgcttgt
     9121 gtcagaacag ttgctgtcgt tgtaccgtct cctgcaatat catttgtttt tgatgctact
     9181 tctgatacaa gttttgcacc catgttttca aaatgatctt ctaattcgat ttcttttgcg
     9241 atcgtcacac catcatttgt gatcaatgga gagccgtaag atttttctag tacgacattt
     9301 cggcctttag gtcctaatgt tactttcact gtatctgcta atttatctac cccgcgtaac
     9361 atagctgcgc gtgcgtcttc tgcaaatttc aattcttttg ccatgattga ttcacctcat
     9421 tgattatttt tgattatttt tttatcttcc aaatttactc aacaatcgcc atgatatctt
     9481 ttcctgcgac gatcagatat tcttttcctt cgtattttac ttctgtaccg gcgtattttt
     9541 cgaacataac ttggtcgcct gcttttacag cggcaggaac tttttcaccg ttttctaaca
     9601 aacggccttc accgactgca acaactgtcc cagtttgggg tttctcttta gcagcagaag
     9661 ctaaaacgat tcctccgaca gttttttctt cttcttttgc gacttcgata atgacgcgat
     9721 cacctaatgg ttttaacacg ataaatccct ccgataactc tttttttaat ctgttagcac
     9781 tcttaacctt cgagtgctaa cctacatttt taataatact cattctccct cttttttgca
     9841 agcttttaaa tcaaagaaaa gaaattttat tctgacagct gcttattgaa attatgatat
     9901 aattgaaatc aatcgatata agcttgcctt gcatgcttgt tatcccttaa aaggaggaat
     9961 tacatgtctt taaaaaaata tagcttcctc agcatttttt gctatggtct aatttttatc
    10021 tcacccttga tcctagcttc gataggactt gtaaaatcta cgtctgaatt gatcacagca
    10081 actgcagtca tgtatatact aggcgccgtt gtcctagcta ttttttattt taagcaacgt
    10141 gaacccttag cgatcgaatc agcggtaaaa aaagcttctg ctccaaagat tgttatttat
    10201 ggattggttg gaatctttgt cgcattgatt ctgcaaagca tcgctgtcat gttagaatct
    10261 gtattgtttg gcaaagcaac cccttccgag aatacacaaa atatcatcca gatgatcatg
    10321 gaagcacccg cttttatttt ggcgacaact gttgctggac ccatcatgga agagttcgta
    10381 ttccgccgca gtatactggg gatcatcagt cgatattcaa atttttgggt cggagcagtg
    10441 atcagctctt tgctgtttgc gtttgctcat aatgatggtc atctgctaat ctactttttc
    10501 ctaggtttct tcttcagcct agagtacaaa gcaactggtc ggatctggac atcgatgatc
    10561 acacacgtag gaatgaacac attagttgtt cttgttcaat tagctgtcca aaaagggcta
    10621 gtataaacat gactataaat actcctgtca tcctagattg gtgattttcc caatctaaag
    10681 cgacaggagt atttttgttt tttagtcgtt catcttttct gctgctccat cattttttgt
    10741 ctgtctttcc tttttgcagt ttgcagaatc tatttttttc aaaaatggga aataaatgaa
    10801 aaaactaatt gacaacacga taccttgtaa aatcgctgca ggcactccgc ctactaagaa
    10861 acccgagagg atcggcggtg ttgtccaagg gacttggact gctgtaaata aaggaacgaa
    10921 tccaaaatat aaagcggaat aggtaatcaa tcctgaaacg accggtgcga aaataaaagg
    10981 aacagccatc atcgggttca tcacgatcgg tgtggaaaaa ataattgatt cattgatgtt
    11041 gaaaaaggct ggtaaaagag caagatttcc taattctctg tatttcttcg atttcgcaaa
    11101 aaccgtcata aaaaatacag cccctaacgt caatccagca cccgtaaccg tcatgaactg
    11161 atcgagaaat tgctgagtaa cgatatgtcc gccatttgcc accgtcaact cttgtcctga
    11221 ggctaagatt tctgcatttg ctaatgaatt cgcttgcaag acgggactaa ccaacccatt
    11281 gaccatcgca gatccatgaa gaccaaataa ccataaaaac ggaacaaaaa aacctaataa
    11341 aagcacacca aacaacgtgt ctatgaacga ttgaatggga agctggacat atgtccatac
    11401 tccttgaaca acagtcattc tgaacaaatg ttgaaaaacc gctgaaccga taagcgtaaa
    11461 agtgactata acaatgattg gaatcaatga agtaaaagac tgccaaacat tttcaggtat
    11521 cttctctctt tgcttccacc ctttcgcaag tcttactaaa cgtgcatagc cccatccgat
    11581 gatgagccca gttgtaactg ctcctagtat cccttcagca cctgtccaga cagtatctat
    11641 ctgagtattt agtggctgaa taagcagtag ataagcagac aaggctgtta tgccgactgg
    11701 taagcgatcg atgtcttctt cttttgcata agcgtatgca atggcaaaaa cgatccaaaa
    11761 ggctatcaca tcaaatgtag cctggtatac ttgtgtaaaa tacatagaaa atcctgtttt
    11821 gtctatccac tgagcgatgt aatccacagg aaaatgagca aataagaaaa acagcgagcc
    11881 aacaataatt attgggatcg tgtaagtcat tccattacgc aaagcagtta agggtctaag
    11941 attcacaaca gatgttatca ttggtaaaaa ggcacgatct aaccactctt tcatttctta
    12001 tctccttcaa cttctatttt tatcaatcag ttttcttaat ttaactatac ccaatgatca
    12061 cgaaccatga aaggaatgcg cactgagctt ttcaaaaatc ggattctttg acgaatattc
    12121 agcaactgct tacaatggac aggaataaaa aaggaggttt tttatgaaaa ttgcactcat
    12181 tggagctact ggccatgcag gttcatttat tttagacgaa gcattgtcca gagatttaga
    12241 cgtgacggca atcgtgcgtc atccagaaaa attgccatca gatgttccgt ttatacaaaa
    12301 ggatttattt gagttgacaa ctactgatct tgcatctttt gatgttgtcg ttgatgcttt
    12361 caacgctccc aaaggaaaag aagaaatgca tcaaacttct ttgtcccatt tgattgagat
    12421 gcttgaagga accgatacac gcttaattgt cgttgggggt gcctcttcgt tattcttaga
    12481 tacagaaaag acgactcgta tgattgacca agtacctaaa aatgcaccct tctatccaac
    12541 agctttcaat atgtacgaag gattgttaaa attgaaacaa tctaaaaatc tacattggac
    12601 ttatatcagt ccagcttctt tatttgatcc acatggcaaa cgtacaggta cttatttgtt
    12661 aagtgatgat tatttacaaa gaaacgctgc tagcgacagt acgatcagca tggcagatta
    12721 cgcgattgca ttagtagatg agatactgga tgagaaacat gaaaagcagc acatcagtgt
    12781 agtcagtcga taatacggtt atttttttaa tatgctaaaa aaataaaaat agaagataaa
    12841 accaaccctg attgatttta tcttctgctt tttatatttt tattgattga actgtttaaa
    12901 tcgaaaatag ctcagttgct tcatctggat ctgtatcata gatttcaaaa tcatgtccaa
    12961 cgccaaaatc tttttctttc aaaacatttt ccattgttaa atgagcaagt tctttcatat
    13021 tgttttcctt gctaagatgg ccaagataga ttcgtttggt ccgattaccg atcacatcag
    13081 tcatgacaag tgcaccgtct tcgttggaaa gatgtcctct atctcctaaa atacgctgtt
    13141 ttaaattcca cggataaggt cccatgcgta acatctcaag atcatgatta ctttccatca
    13201 aataaccatc ggcattttca atgacacctc gcatataatc actgcaatat ccggtgtccg
    13261 tcaacatgac gaatgaccga ttttctttat ggaaacgata aaattgcgga gccgctgcgt
    13321 catgggagac gccgaaactc tcaatatcga tatcgccgaa tgttctgact ttccccatct
    13381 caaatagatg tttttgttcc actggtacat ttccaatcag tggttccatc gcttcccaag
    13441 tctgctgatt ggcaaaaaca tccaaatgat acttacgagc caagacaccc acgccatgta
    13501 tatgatctcg atgttcatgc gtcacaagta ttccatctaa ttggtccggt gtccgatcaa
    13561 tttgagccat caaggaagtg atttttttac cgctaagtcc tgcatcgacc aaaagccgtt
    13621 ttttttcact ttcgatataa agtgaattac ccgaacttcc acttgctagg atactgattt
    13681 taaaagctgg tttttcttca gccatttgac tcctacttcc cttcttactt tgtaaacttt
    13741 tttcattata ctcgcttttt tattgatctt ccacttttgg tacagtattg tttgtcacga
    13801 tcgtattgct gaaagcgttg acgctctcaa tttggaccac tttatcgctt gtttcgatcg
    13861 ctacatgcca aacaggaaca tacacatttt tttcccggac tttcagaata caggaataag
    13921 ctagctgacg ccataaaatt tttgatcctc ttggtatttt gttattgatg tacaatgtat
    13981 tgattgcttc ctcttccgta tacagttccg tcttttcgcg cagctgctcg atatcagata
    14041 aatgcgtctg catgtatttc gtgatgctat aatcattatc gtttttttct aaagaaagaa
    14101 cgatttttgc tgtatcatca ttgaatggga tgccttcata ctcttggctt gcttggattt
    14161 ctagtggttc ttcttctaaa tgagaaaagt ttttcgtgta cacataatca ttaccgaata
    14221 aaatattgtc tttgttagat aaaaaatctt ccaaggaact actaatttta tctggatcga
    14281 tcacgttctt ttcagcatct gaactggata aagatttcgt caaaacatca ccgtctaaaa
    14341 acacaccact tgccagcaag ccgcttttac cagccttgcg ataattcgct aatgaagtgc
    14401 taagattggt ctgctccgcg ctaagataat agccttgctt gttctcagac gaaatcttcc
    14461 ccgtgtaagt gatatcgtcg ctagccagac gttgttggat tgattcagtc tgattagagc
    14521 gaatgacatt gttttcttgg tataccgcgt catgataact gctgaataaa aacacattca
    14581 accctaaaaa agcgacgaaa aatatccatt cgattttttt gaaatccaat taattcgcct
    14641 ccaatccagg taatttttcc agaaggtcgt tagctgaata ccagttatct ttatatttca
    14701 cataccattc aggtaagaga tcaacgacac gattcgtttc tttgacattt tgccatgtat
    14761 agccaacgat cactgaacca atcttgttgc tatctgctcc cttagctttt aactgctgga
    14821 tgatttcatt cgtacttgca agttctactt cttcgtctga aggtatcggc acttgaatcg
    14881 tattcaaact ggtttggata ttcacttgca cagaaccatt cgtttcaccg ccaacttcca
    14941 caccaatctg tcctctgctg tctgtcccaa aaaccgggaa gccttccacg aatatccgat
    15001 aatcgatcgt attgtcactg cggtcaaaat atctgaggtt ccctaaagaa ctgcctaatt
    15061 tgctaatata ggggaaactt tcagaaaata cactagcatt ttcttttttc tctggcagct
    15121 ctccacgaaa atcaactact tgcgtttccg aatggataga cattgtttca ttcccaccgt
    15181 aaaaaatcaa ttcattgctt tcatcattat ttttcacgct atctggtttc tggaaaaaag
    15241 catttctaaa aagtgtatag ggttgctgtg acaggatata actatacttc ttcagtttga
    15301 tcgaatgttt cgtagcatat tgcgtgtcga tcgtcgatgg ttctgttgtc atttctgtcc
    15361 attttgtgtc ttttgctgtt aattctttat tgatatctgt ccaattcaac gaaatatttg
    15421 cttcatatac gagatggttc gaataattca aaaatcggat ttttttattt ccttgatcga
    15481 attgtattct agtaaaatac atattttcgc tttcttttgc caagttcgac acatctaaat
    15541 ctaaatcaaa tgcttccttg tattctctta gcaaaaaagg cgcattatag gccaactcaa
    15601 aaccagagtt cagatttttc acctgttcaa actgtgattt gtctcctgaa actacttcag
    15661 aaagctttcc aaatcttgct ttatcgatga tcaattgcaa tcggtacacg agattttcac
    15721 tgttggtttc ttggatcatt cccgaatggt gccatgtaac atgaagcgga agaaagactt
    15781 cggaagtttt tcgattgctt tgagactcga tcatttgagt gtttgtctca tctacgttga
    15841 ttgaactttt tcctgctggg ctcagccaga ttgcataaga gaagtatatg cttaacgcaa
    15901 ccatcaaaat gagtcctata cgaacgactt tttcagagat tctcattccc accattcctc
    15961 ctcataaggt tcatatggca gcgagatata aaaagtcgac ccttttcctt ccttgctctc
    16021 tgcccaaata gcgcctcgat gcgccttaat cacttctcta gaaatagcta atccaagtcc
    16081 tgtcccacct tgtgctcttg cacgtgcttt atctactcga tagaaccgct cgaacactcg
    16141 gttcagatct tttttaggaa tccccaatcc ttgatcggtg atacttaaaa tcacgttatt
    16201 atgcgtttct aataagcgac aagtaatcgt tcccccatct ggtgaatact tgatcgcgtt
    16261 attcatgata ttatcgacta cttggatgat tttatctgta tctacttcga cccaaagatc
    16321 tctttgcgta aattcacgac ggatcttata atttttttcc tgatttccca ccatcatatc
    16381 aaaccggtca agaacaaaat tcactaactc gttgaaattg acatattcta attgaagctg
    16441 ggtattacct gtatccatgc gagatagatt taagaggtca ttgatcattc gaatcattcg
    16501 gtctgtttca tctagcgtta ccttcaaaaa atttggtgct acttcctcgt ctttccacgc
    16561 accttcgctc aatgcttcga tataactacg catacttgtc aacggtgtac gaagttcatg
    16621 ggaaacatta gagacgaatt cacgacgttc ccgttctgtt ttttcttgtt ctgtcacatc
    16681 atgcaagaca gctactaatc cgctgataaa acctgattcg cgacggatca tagaaaagtc
    16741 tactcgcaag atcatctgat cactttccaa tgtactggtt gaacggtcaa gtaccatttc
    16801 gtttggatct tctaataatt tccgcaacgt atattcttct tcgatatcta aaagtgtcaa
    16861 aatcgattgt ccgattgctt cctcgttttt gatatctagc aaggaaacag ccatatcatt
    16921 gatcgtaatg actttacccc tgcgatctgt cgcaatgacg ccgtctgtca tatgggatag
    16981 cacgctatcc aaacgattgc gttcagcttc cattgtatct tgagcttctt caatacgttc
    17041 agctaactga ttgaaggtat cagccagctg tccaagttca tcttgtccat gcactttgac
    17101 tttccgactg tagtctcctc tagcgattcg aatggcttgt tctctcattt caccgatcgg
    17161 ctgtgtaatc gaacgagcaa ctaaaacaga tacgatcatt gaaataaatg ccgcaattag
    17221 tgaggcagta aagaaaatga ctgctgtgtt attgatttcc gaatattttt gttcgatatt
    17281 acttttgacg taaagagccc cgataacggc atcgccggtc ggggacagga tcggttgaac
    17341 attgatattc acccgtttat cgtcgttatc taaagcaaca taacgtttag tcgtgaaatc
    17401 attgatatca acataatcat ttttctttcc cacagcgctt tgttcattga catcggttgt
    17461 tcctcggatg atcccttttt cgtcaacgac ccgtatttcg atgatatcgc tagtcgcact
    17521 gttttcaata aatcttttga tattcgcatt tacctcttca tcatctgcat tatcttttcg
    17581 attcaattcc gggctgattg ttgttgctaa tgattccacc gtttgattca tatctttaat
    17641 aaatgtattg atcgtggaac gttctaaacc acgtataaag taggcaccaa taatttcgat
    17701 cgagatcaat aaaatcagaa taaaggtaat agcgatctta aaattgactg atcgaaaaaa
    17761 gcggactttt cttttcatcc aattactcct gttctgggtt acgcaaatag taaccgacac
    17821 cacgacgtgt cactaaataa tttggatggc ttggattatc ttcgatcttt tcacgcaaac
    17881 gacgtacagt tacatccact gttcgtacat ctccaaaata atcataaccc caaaccgttt
    17941 gtaataaatg ttctcttgtc atcacttgtc caaggtgctt cgccaagtaa aagagtagtt
    18001 cgaattcacg atgtgttaac tcgattgttt ctcctcgttt cgtcaccatg taagcttctg
    18061 gatggatcgt caaatcacca atcgtcaatt ccgcaggtgc tgcctcttct ggttccttcg
    18121 cagccgtggc accgcgacga agatttgctt tgacacgtgc aactaattct cggttggaaa
    18181 atggcttggt cacgtaatca tcggcgccta actctaagcc taggacttta tcaatttcag
    18241 aatctttggc tgttaccata atgatcggca tatcataagt tttgcgtacc tcacgagcaa
    18301 cttctaagcc gtccatttta ggtagcatca aatcaagtaa aatcaaatca ggttctacct
    18361 cagcaacttt ttccaaggct tcttccccat catatgcagt ataaacgtca tagccttctt
    18421 tggctaaatt gaatttgacg atatccgaaa tcggcttctc atcatcgaca actaaaactt
    18481 ttttcataaa gagccacctc ttatatatta ttgtcatacc ttctcattat accgtatttc
    18541 tgcttttgtt tcatctttgc ctataaaaca aaagagaaaa ttaaaatgaa agtgcaaaat
    18601 actttattat tttttgttct aacttcaata aaaaaggaaa acactgattt tcacctcagt
    18661 gtttcctttt tttgatttat ccttctaaaa cttgtacacc gacacccaat aatcccggac
    18721 cagtgtggac tactaatgcc ggagaaatct ggccaaagta gatttggttt gcttgtggga
    18781 actctctttt aagtctcgct tccatttcaa ttgcttcttc tttcgcatcc ccatgggcaa
    18841 cagctaagtt gaagaccttt gcattaccga ttttttcttt gacgtaagca accgttttat
    18901 ccaaactttt tttacgtccc ctagcttttc ctactgtgta ataaatccct tcttcattac
    18961 atgagatgat tgggttaagc tttaaagcgg ttcctacaat agaagcaact aaaccgatcc
    19021 gtccaccttt ttgaagatat tccaatgtcg caacattaaa gaaaactttt gttctttcga
    19081 ctaactcatt cagattactg atcagttgat cccattctac tccttcttcc agccattttg
    19141 ctgcttggat cgcagaaaaa cctgcaccaa taccaatatt tttcgtatct agtacatatg
    19201 cttccaattc ttcttgctgt tgtgcaatta aacgaaccac attatatgtc ccgctcaatc
    19261 cactggaaat tgttaccgca agtacctttt cataaccgtc ttttttgatt tgctcaaaga
    19321 tttctgtgat cgactcacca tccggcaaag atgtaccagg gatttcctct ggcaaacgtt
    19381 gatagatctc ttcaggggta atgtcaattt tatctgtata tacctgatct ttataaataa
    19441 ttttcaatgg gatcatatac atctcgtatt tttttatcaa ttcttctgga acatccgtac
    19501 ctgaatctac taaaattgcg attttattgt ttttcatttg ttttctcctc ttttaaatat
    19561 tcggtttcta gaacaatcgt tttttccgca agaagtttca ttgcaaacga taaagttgcc
    19621 gctcttacag caatatattc tacaggggtt gcctggtcga atttttgcgg atgagattta
    19681 ccttgtgcta attggctgac catccacaca gctgcttctt gttcatcgca aaatagatta
    19741 taggcttcac gaattcctac tgtttttcct tggaacaata tcccttcttt gatttcagga
    19801 atagtcaata cttgtttcaa cgtagtgatt gcaagtaaaa atgctacgtg ttctttgtta
    19861 tatctttttt taactggtgc tggtatcaaa cctagtttaa cgtaattgtt taccatagaa
    19921 gaggtaagca atggatgctt ttctccttgt atcacaggtg aaagataacg atcaactaac
    19981 gttctgactt ggtccatata aagttctaag tcagggagtt cttcccagtg aggcagacgg
    20041 aatgtttcta gttccttccc ccactccatt aattgctgac gcattttttc catcataacc
    20101 gctccttttt tcatttcata aacatgatat cagacttatg ataaacattc aatctagttg
    20161 ctttaactag gtgatttgat tagcgggatt ccatgaaaac aaaaaacaag aaggctaagc
    20221 cttcttgtct tttatcgtta agaacaggag acccgccggg gagacggcgg gaaaggagta
    20281 aaaaatgaaa aaagtgttag ggtttgttct cttgcaagaa ctttgtttgt tggtatgctt
    20341 atataatagc gagcaaatgt gaataatcta tgaatgattt cctataagga agtaaagatt
    20401 tttctgattt tctttaaatg tagtcttgca ttttaaaatt cttagtgcta ctattattaa
    20461 gtactttttg tacgggccgt tagctcagtt ggtagagcag ctgactctta accaaatgct
    20521 ctatttgtga aaaaagtttt ataaagcttt ataaataggt ttttagtttg tgaatttttt
    20581 cattgccaac atactgccaa caaattgcca acaaaaaaat tctaaaaaca agaagcgata
    20641 ccccaaagat attgtttttt gtttttttat tctttaaacc caaatgcgtt ttctaatgct
    20701 tctgctgcac gatcttttac tcctgcggtt aaatgcgtgt ataaattagc agttgtcgaa
    20761 tacgatgcat gtcccagcca ttcttgaatg gatttcatat tctggccact atctaacatt
    20821 attgaagcat tagaatgacg caagtcatga aagcgaattt ttttcaatcc atttttctta
    20881 atcaacttac tcaaacgatg cgttacatat tgaggcttca tcaaccgtcc atcttccata
    20941 gtaaatacgt actcattatc ttctaaaata taatcatctc ctaaaagaat acgattatct
    21001 tgttgccatt tagcttgatt tactaatgct cgttcaatcg tttcaaccaa aggtaatgta
    21061 cgatagctgg aagagttctt tgtttgatct ttctttatta actgtgacat attctctgga
    21121 ttttctatga ttacatggtt tatcatgatt tttttgttta aaaaatcaat agcagaccat
    21181 tttaatccta aaacttcact tcttcgcaat ccataaaatg ctgttaaaag aactaccaaa
    21241 tagagttttt cttctgacaa aatgtcaagt aatttcaaga tctcttgttg attataaact
    21301 tgagaaacaa attgtttctt ttttggcttt cttactttat ctgctacatt tgtacttatc
    21361 aattctgtaa taacagcctc ttgaagagct ttgcgtataa atgcgtgata atgaataacc
    21421 gtatttgctg tacagttatc atttaacata tattgataaa actcttgtaa atgataaggc
    21481 tttaacctat ttagtttaat ttttttagga taaaaaaact tgtaaattcg attatggacg
    21541 taatgcttat acccaacata tgtgttaaat gctacttgtt tttttgatac ttccaaataa
    21601 ttaagcaaat agtcataaaa caataaattt ggattagcac attcacattc catagctaat
    21661 tgactttcca attctagtaa aaattcatct ctaatttttt ctgctcgttt tttatttcct
    21721 ttctttttta attttgtatc tttccacttt tgtctaattt ttccgttaga gtctttataa
    21781 gatacaacta cttgtaatgt tcctcttttt tcaattatgt ttgctcttaa tttcaattca
    21841 atctctcctc ttaaactgaa atatcggcaa aaattttgac tattttatta tagcatgatt
    21901 tttaccttca tcttgtattt tcaatatata ctcaatgaca gcttttttag gtattttgta
    21961 ccttgctcca atttttatat tttctattga tctatctttc agcaaattat aagctttatt
    22021 ttttccaata tttaacattt tagttaaatc agtaattttc aatatcgatg gataactttc
    22081 aaatattttt tcataatctt gaaaatccat aaaattcact ctcaattctg ataataagta
    22141 ttgcactttt ttattttagg tgttagaata acaaagtatt aatttgggct gttagctcag
    22201 ttggtagagc agatgactct taatcatcgg gtcgcgggtt cgagcccctc acagcccatt
    22261 gggtgccaaa cccttaaata cagtgtaaac gttggtgctt agcatttaga aaacgtatat
    22321 actgtatttt tttgttttca aaagagactg tagtacccct ttttttcact aaaagcccac
    22381 tgtagtcatt gatatttttg ataagaaagg aacataatta tgcgtaatga aattgctaat
    22441 ataacaataa tccgagaaac tttacaaaac catactgcaa atgagatctc taaacataca
    22501 ggattaaatt tgagtactat aaaaaaacta aaaagtggag aacgttcaat agaaaaacta
    22561 aatttacatg atgctatttg cctcactgaa tttggtctta aaaataatag aaaaaatgtt
    22621 gaaatcaaca tatggaaata attcttaatc agctgctttt gtcaaaagca cataggaatt
    22681 tcacctctct ccaagtttac ggcgagcctt acggaagtat cattatccca acttcaaatc
    22741 atcgcttttt attgactata aaaattattt tagaatgttt atcgctcctt ctttacagta
    22801 agaaatcatg gcgcaactaa aatatgctcc ttgaagtgtt aacgtactga tagaatgtat
    22861 ttaaatttca atgatcaata ggttttctgt ttaaaagaat ctactaatcc tttaactatt
    22921 agaagattct tttaaacaga ataagtggaa aggggacttt ttagttagtt aattttaaat
    22981 tttaagatcg cttctatcaa tttaatttct agctgtctta gcatatactc atctacatag
    23041 gtaataaatt gccccgcctc attaaatcca tttgtcaaac ataacttagc tatataacta
    23101 tgataatgtt tgagtatcat cttgattgaa attggatctt cttctagagc agattggata
    23161 atggatagag gaactaatgg atactgccgt tttttggggt tgtctaccat aagtcatacc
    23221 ccatttttaa ttgttcaact gccttctttc gttgatacca gataccactt gtactcatat
    23281 tgaggatttt accgatttca gtatcattaa aacctgcaaa ataataaagt aggataacct
    23341 ttcttcttat ttccgataaa tgatcaatgg ctgccgataa cttttgatct gaaattaata
    23401 tttccattcc aaataagaaa tagacatctt gttcatcaaa atgagtttgg gagtacttac
    23461 cttcatttga attcgtcaat ggttcctcat tatctgttat gttaatgttt cgttttcgaa
    23521 tagacttagt ttcgtttcgt aatacttttt tacagtaggc atcaaattga tgttgtatag
    23581 atttctcatt gtaattttgt tgcatgataa atatgctcct tttttcccct ttccactaat
    23641 acaacaatca gaaagcgcct ttttttcgaa aaaactagaa aaaaatatcc ttctttaaac
    23701 agaaaatctt tttataaaaa aaaggagttg gcaattaagc agccactcct tttttatatt
    23761 ccttataaaa ggtaaattat tttttataac tgaaatttgg tattgatgac catcgtttct
    23821 ttagatagtg agcaatcatt ttaggctttc tatctctagt aaagattcct ttcttatttc
    23881 cttgcactct taaaatccct tgacttgttt caaaatctgc aaaattccac acttgttcac
    23941 caacaaaatt gtcacattta tctattactt cgtgatttgc ttcgtaataa cccacttgat
    24001 actcttctgt aaacattgtt ggtactgtat catgaagtcc agctactgta tctgctccat
    24061 attctgtaaa cattattggc gtatcgggac atctttccat ccatccatct agctcttttt
    24121 tcaacagata ttttgcatgt tttaggtctc cacctgaaac ataccaacca taatagcgat
    24181 ttaaacataa aacatcgaca atatctccta ctttacaaat atctggtgtt gactttaagt
    24241 aagtaacgat tgttacaggt ctcttctgag ggtcaagttc tttagtcaat tcaactaaag
    24301 gttcaaaata ttctttagcg ccttcagaat ccgaatctgg ttcatttgca atagaccaca
    24361 ttacgacact cggatggttc ttgtcgcgtt ctatcaattc cttaatcgcc attttgtgag
    24421 cattttctgt ccgtatttct ttccaagtat catgtttttc tacgttgtca tctaataaag
    24481 tagccatgaa attcaaatgt aatcctactg caggtacttc atcaataact acaatacctt
    24541 ctcgatctgc taagcgcata atttcttctg aataaggata atgtgaagtc ctaaatgaat
    24601 tagcaccgat ccatttcatt aaattaaaat ctttgatatt tactgcttca ttcaaaccac
    24661 gaccattaat atacgtgtcc tcatgcttcc caaatccctt aaagtaaaaa ggtttattat
    24721 taatcaaaaa cttcccattc gaaacctgaa tactacgaat gccaaattct tctgtgtacg
    24781 tatcaatcac tttattttcg ttaattaaac taactttcat actatataag taagcattta
    24841 gaggctccca aagtgttggg ttcttaagtt gaatcactcc ttctttcccc ttagcttctg
    24901 ctataagttg agcttcttcg tcatagattg ctattagaat ttctccggtc cattctgtat
    24961 cgatattaaa ttctacattt gcatctgaat caattatttt tgtattaata gtgacatcct
    25021 ttatatagtc tttcggagtt gtatacaatt taacgggtct attaatgcct gcataattga
    25081 aaaagtcaaa attaaccaaa ttttcttttt ttatgtctcc atcttcctgt atcgtttctt
    25141 tcaaaatccc tacaggtaaa gtgctatcgt caataacatt gtttacagcc actgtaattc
    25201 tgttacttcc atcttttata aactgattaa tttctgattc aaatggcgta aaccctcctt
    25261 tgtgttgatt aatcaatttt ccattcaaaa atacttttgc ttcatgtgtt gcagatccaa
    25321 aacgaagaac taatctttct tctaataatt ttttaggaag aatcacttct ttttcatacc
    25381 acacccatcc aacatgattt ctaatgttct ctgtttcaaa taaatcatta taagaagaag
    25441 gaactgaaat tggataactc tctttttcaa ccattggttc taaagcaagt tcttcttgta
    25501 agtcatcatt tgtatctaac ttaaatctcc aaagaccgct taaatcaatt acttgcctcg
    25561 tctctgtcat aatgggatat aacatgtaaa tcactcctat ataatttaaa tattaaataa
    25621 atgtatcctt taacccacaa taagcatcta ttggacttat tcccttaaat tcagaaaggc
    25681 ccattagttt gtccactaat atttctaaag tttcttgttt tccatcataa gtattgatat
    25741 aagtttttac ttgaggcaca tcagctaaat gaaacggaca ctgtacagat acaaaaattg
    25801 ttggaatttc atgaatataa aatggaatat ccggagtacc tttacttgct ggccaaacaa
    25861 ttctttgaac tgtacctggg ttaacattag ctatattaat aatcaagtca tatttttcag
    25921 ttaaatcttt gattggtctc ttagcagcat atattccagc tatattttta attttttctt
    25981 cttgcgataa tcctttcaat tcttcttcag gtgaaatgta tttttcaact tcaaatcctt
    26041 ttgctgacaa aatctgtttc aataaatcaa tggcctgagg tctatttcca gcaactactt
    26101 caccaaaccc accttttacc ccatgtacat ctaccagtaa aatcctttta tatttttctg
    26161 gtacaatagg ccaaatatct tgtttatctt taactagtgt aattgcttga tcagctacct
    26221 ctttaaaaat tgatttattt tcaattaaac caattttttc catagcttct tgtttcggta
    26281 acaaaatttt attcttttct tttttatgca aacctagttt agcctttaag cctaaaattc
    26341 ttcttaatgc atcacgtaaa cgctcttctg aaataattcc tttgtaataa ccttccatca
    26401 tataattaaa gtcttcatct ggatcattaa aaaataggaa tagatcacat ccagccgcaa
    26461 tagaagtggg caataattct gaccgtttca ttgccccagt cattcctaac atatgactag
    26521 catcagtaac tactaatcca ttaaacccga gttttcctct aagtaagtca gtgattaatt
    26581 cttttgataa agttgcagga agtaaatctt catctttcag atcaagatta aaatgttttg
    26641 aataagctgg catatggata tgacctgcca taattgaagg taatccagct tctattaagc
    26701 ctgagtatac ttttccaaaa gaattatccc attcttctgt acttaaacta ttaatactaa
    26761 aagatagatg ttgatctcgt tcgtcaatac cgtcaccagg gaaatgttta gctgcaggca
    26821 aaattccact ctcttgaata cctttcatat aagctaaact aagttctaaa actttatcag
    26881 ggctttgtcc aaaagatcga ctagaaatga taggatttct ccaatttcta ttaatatcta
    26941 caataggagc aaaactccag ttacatccaa tagccgatgc ttcaattcca gaaactctcc
    27001 ctaattcata agcatatttc gcatcatttg ttgcaccaat ttttacttct acaccaactt
    27061 cggtaccatc tgtacaagct ccgttaccac ctgcttctgt attagcagca attaataaag
    27121 gaattttgct ttctgtttgt aggatatagt tttgatcata tacctcatca gcagttcctg
    27181 gattatatcg tacagctcca atatggtact gattgaccat actacttaag taatttttat
    27241 cacgactaga tcccatatta acaaataatt gacctatttt ttcttctaaa gtcatagcat
    27301 caatcgtctc ttcaatccaa gaaatagcat cttcatctaa atgatatggt ttttttcgta
    27361 agtctacttt tatagtcatt ttctttctcc tctacttatt agtaagattt ttccaaaaag
    27421 tatctatata tttttgttca atatctatat actctcccga taaacattta aataaaatat
    27481 cagttcttaa gttttcccat gattcttttt ctttattaac caaaatagga taaaagaaaa
    27541 ctttattctt atttgcagct tctagatcac caggagaatc accaatcatt aatatcttat
    27601 ccaaatcaca accattttca attacacttc gaataatgtc ttcttttttt cctttatctt
    27661 gacaacacag ttcatcaact aaggataata attcatgtct ttcccattct tcttgaatag
    27721 cttccttatt agctgagctt acaataatta tcttcccata ttttttcaga gcttttatag
    27781 tatctctagc acttaaaaag ggtttatcta atcctttagc ttgactaatc tgtcgattga
    27841 cttcttccga ccattttaaa gcttttaata aatcctctga ctgagagtat tctatttccc
    27901 tcttcaaaga tggattagac aattcttttg ttacagatac ccagtgcttt aatcgattaa
    27961 tcgatggtac atcatttttt tctaatgttt taactaaacc taaaaatctg tttattccac
    28021 gagtctctga atataaatta atattattcc aatcctgtag gaatttttcc ctatgatgaa
    28081 catcaaaaat ctctgcagca attggcccga aaaagagatt gtgtttataa gtcattgtat
    28141 ccatgacaca accatctgaa tcaatacaaa atatccaact gttcatgttt tttctcctag
    28201 ttatgttttt gatttagcaa attataattt tctaatactt tttctttaga taatggatat
    28261 acgaaaaata aaatcaatcc tacaacaaag aaacaaactg ctggaatcaa cgttgaagag
    28321 ttatacagct tttgtaaaac ttctccactt tgtacttttg ctaacgaatc ataccctatt
    28381 atagaaagag tccaacctcc gacaccgcca gctaaagctt gtccaatttt tcgggcaaaa
    28441 gaatatactg cataaatagt tccatcttct ctttggccag atttcacttc ttgatcatcg
    28501 attacatcag taatattcgc ccaaatgact gtattaaaaa tacctaaccc catatatcca
    28561 acagaagtaa gaactaagaa tacatacata ttactaggct ttatcaaaaa taaaagtaaa
    28621 tatacaatag cagagaagaa catccctact gacgccaatt cttttttacc gtatttttta
    28681 ctgaaaatta aacttacagg tgaaacaagt accaacatca aaataggatt gataaagttc
    28741 attaaaacta ttccctgtgc attattataa taatctggaa atacataatt gttcatactc
    28801 attgttaaaa gctgagcaag caacaaaaaa atagctgcag ctattataga aagcaacgct
    28861 ctatttttag ctaatccttt taaagatttc aacaatgaaa cttttaactg cgattcatca
    28921 cttttaaccc gttctacagt taatttataa catattacgt aacaaataac tgcacacact
    28981 gaaaatactc ctgccacaat tgtaaaagtt gatccaccac gtacaatttg agctccagaa
    29041 ctatctcttg tataaataaa aattggtgtt aacacgccaa tgaacatacc tgctaaggta
    29101 gctcctatac tacgaaatgt tgataacgct gtcctttctt ctgcaacaga agtgatagct
    29161 gatgccattg aaccgtaagg aatattaata gatgtataaa aaatacttcc ccatagtata
    29221 tatgttacat acatatatat aactttaata ctcatagaag ctgatgccat tgatgtttga
    29281 tacattaaaa aacttgctaa agcgactgga ccactcatcc gtaaaatcca cggtttaaat
    29341 ttgccagttt tagatggttt tgatccatcc acaatacgcc ccattgttat gtctgtaact
    29401 gcatcaataa atcttgctac taaaaataat gttcctacgg tagctccgct tatccccaat
    29461 acttttgtat aaaaaatcat taaaaaagaa ctagcaaaaa tgaaagtgaa atcattccca
    29521 aaatctccaa acatatatcc tattttgtct cttagaccaa attttctttc catataattt
    29581 gcgctccttc ctattgttaa gcgttttcat tctgttgaac tgccaactta cgtactatac
    29641 tctgaagtat aaagagttag tatttatttt ttacatttta tagtattatt tattttttct
    29701 cctaataatt ctttatctca taaaacttat ctatatattt actagctatt cgagttactt
    29761 cttcaaaatc acctttttca acaggagcca acaaattccc acctacacca actgcaacaa
    29821 cgcctgcttc aaaccactct tctaagttat ctagacttac tccgccagta ggcattatat
    29881 ttacatatgg taatggagct tgatacgttt ttacaacaga tggaccaaaa gcagaacctg
    29941 gaaatagttt aatgatttct actccagcgg taagtgcctc ctttatctca gtaattgtca
    30001 tacaacctgg taaataagga atttgatata aattacaaat ctctgctgtt tgtcgatcaa
    30061 aagatggact tacgatatat tctgctccag ccataattgc aatacgagca gtaaacgcat
    30121 ctaaaactgt accggctcca attattacat ctttttgatc agcatatttt ttagcaattt
    30181 ctttgattat ttcttcagca ttcggaactg taaaagttag ttcaatccct ttaattccac
    30241 ccgcagtaat tgcatttatt gctttaatgg cctcatcttt attttttcca cgtacaaccg
    30301 caatgactcc agccgcttct aacttactta aaatttctac ttttttcata tctatctcct
    30361 tcttttatgg atacatactc tagctagttg tactaactta caataaataa atttaagctt
    30421 ttattttttg tattattttc tactttcgat ttcaaaaaaa ttaaaataat tatacgcatt
    30481 acgataacaa atattctcca ctatctgact aagatacatt tcatcatctg gaattcgacc
    30541 tgctttaacc cactcgctta gtaattgaca taatactcga cggaaatact catggcgagg
    30601 atagcttaga aaacttcgag aatcagtcaa catacctgta aaattcgaca acaaactttg
    30661 ttgcgccatc gtagtcaact gttgacgcat tccttcagct gtatcattga accaccaagc
    30721 acatcccaat tggatttttt gaatcatacc cccttggaag ctttgcattg ctgttgctag
    30781 tggaagccaa tcggttggat ttaatgaata taaaattgtt tttggtaagc acccactctt
    30841 ttcagcagtg tcgaacaatg ctacaacatt ttgtgtcaaa ctagcttgat cccccatcga
    30901 atcaccaccg acatctactc caatttgttc ctttaatata cttgaattat ttcttaaagc
    30961 atttaaatgc atttgcatcg tccaaccata tcttgtatac tcagacatca aatgtaattg
    31021 aatacctgta acaaattgtg caatttctga taaatggaaa tccgtttttc ctgctaaagc
    31081 ttttattaac agattgtcga gtattttttc atctgctatt tcataaaaat aagtattcaa
    31141 accgtgatct gctaatcgac agccaatttg atgaaaataa tccactcgtt ggctcaatgc
    31201 ctgcttcaac ccattaaagt ttttgattgg tatacccgct gcttgactaa gttttaaaat
    31261 atagttcgaa aatcctgatg atttgatact ccagacctta tctggacgga aagtaggtaa
    31321 taccttgaaa gaattatcct gtttagaaag ctcagtatga tatcgcaagt catctgtagg
    31381 atcatccgtt gtacaaatgg ctttcacatc catcatcttc agcaattctt ttgggcgata
    31441 ggaatccgtt tggatttttt cgttggcacg tcgccaaatt tctggtgcat tcttctcaga
    31501 aattgtcaaa tcaatatcaa agtaacgttg taattccaaa tgagaccatt caaaaatagg
    31561 attacctggc gccttctcca ctgtttttac aaaagctaaa aatttttcat aatcattacc
    31621 tgcaccactg atcaactctt cttctatccc attagctcgc atcaatcgcc atttataatg
    31681 atctccagct cctttatcat ttaaccatac ctgagtcaaa ttcttgtatt gcttattttc
    31741 tagcaaacat gctgggtcta aatgacaatg ataatcgatg attggttggt gttctgcaaa
    31801 tttatgaaaa agtcttttag ctatttcatt ctctaataaa aagttcttat ctaaaaacat
    31861 atttaagcac tcctttcctt cccttagttt ttagaagaga aaatttttct tctaaataga
    31921 ctccattata taattattta ctcgccccct tttcaagcgc ttcccaaata ccatttaaat
    31981 aaactgcacc tagagctcgg tcatataacc cataaccagg tctaccggtt tctccccaaa
    32041 tcatccgacc atgatctgga cgaatatagc cattaaatcc attatcgtac attgttttta
    32101 atatcttata catatctaga gaaccatatt ctgatttatg cggcgcttca taaaaatcga
    32161 gtccattatc acttaaatat ttgatattac gaacatgtgc aaaaggaatc cgatcccgtt
    32221 gacaaaattc agatagaatt tcatagacat cattatctgg tcgttctgca atacttccag
    32281 tacataacgt aataccatta gaaggagaat ccactacatt acacaaccat tctaaatcgt
    32341 cacgattttt aacaatacga ggtaatccga aaatagaata tggtggatca tctggatgaa
    32401 ctgccatttt aatcccaact tcttcacatg tcgggataat tgctttgaga aaatatgcaa
    32461 agttctctcg caatttcgct tcatcaactt ctttataggc ttctaataat tgcttgattt
    32521 gtgataaccg atgaggttcc caacccggca attcaaaatt attagtagaa ttttctactt
    32581 ttctaataat ttcctctgga tcagtaggaa tattttttga aatataggct aacgtagaag
    32641 acccatcatc taaatgataa tccaaatctg attttaccca atcaaagact ggcataaaat
    32701 tataacagat aacctcaatc ccaaattctg ccaaattgcg aatcgtctgt ttataattct
    32761 cgatgtattg atctcgtgtt ggcaaaccaa ttttgatatc atcatgaaca ttaacactct
    32821 caataacttt caaggtaagg ccattggctt cgacttcatc ttttaaagct ttaattcgtt
    32881 ctctcggcca gacctctccg gctggtatat ccattaatgt tccaacaatt cctttcattg
    32941 ctgggatctg acgaatttct tgtagtgaga ttttttcttc atttgcccca taccatctaa
    33001 aagtcatttc catagttact ttcctccaac ttctgttata tatttattga ttgttttacg
    33061 aactgcttga ggacctacta gcatttcgca aaagattttt tcaattttct ttcctatctc
    33121 aacttgatat aaatctaaac caaatatttc cttattttct aagatttcct gtaccgcaca
    33181 atgaatcttt gcaggatctt gttcacctaa tttcaatact gagaattttt cctgcaactc
    33241 taccaataaa ggatcaggac ttggcacaaa attttttcct tgatcatcga ctgccaataa
    33301 ataacgtaac caagcagcaa tagcgatagg aataaatgtt agattttctg catttccgtc
    33361 tttttcgaca tactttttaa ttgtttcacc atagcgaatc gctacttttt gagaagtatc
    33421 tgttgcaatt cgttgcggtg tatctggaat atagggatta ggcaaacgtt tattgattac
    33481 ttcatcaata aattgttttg gatcaataac gcctggactt tcaactactg gtaagccttc
    33541 tacataacct atccctttaa ttaagccaac caaatcatca tcttgcattt ctgcctgaat
    33601 tgatttatac cccaacaaac aacctgttat tgctaatgcc gtatggagcg gatttaaaca
    33661 tgtcgtaacc ttcatgatat ctgctttatc aactgtttct ctatctgtca taatcacacc
    33721 agccgcttcc agtttaggtc gaccatttgg aaaactatct tcaatgacta gataatggac
    33781 tttttcagta ttggtaaaag cagcgatatt ggtatttttc gcagtatgaa ttatatctaa
    33841 gtcttcgaaa cccgccttca ataaatccat tttgacactt tccgaaggat ttggcgtaat
    33901 tctatcaatc atactccaag gaaaagtcac tttttcttta tcctttaaat aagcaataaa
    33961 tttttcagaa acgtatcctt ttttttgcca agcttctgca atcataaaaa tgctatcttg
    34021 aaaacgttga ccattctgtg agaaattatc agtagaaacc atcgcaatcg gtaactctcc
    34081 agcattaaac ctgctataca acaaggatgt cacaatactc atcgtatgtt gaggattggt
    34141 aggtccagat tttatatctg cttccactac cgaaaaaaat tgtcctttag aatcttttaa
    34201 actgtatcct ttttcagtaa tagtaaacgt tgtaaactgt aatgtaggat cttcaaaata
    34261 tttaaccact ctttgatagt ccgttatatt ctttggattg caatataaac tatcagctgt
    34321 tactgctaaa agtctttttt tgagttcacc atcttcaccc ataacaatct ccaaaatatc
    34381 attgttgtag tcatgataag ctttttctac tacttcttca tcaaatgtct cacacacaac
    34441 tactccggat tctaattcat tttgatctgc taagcattga gcaatttctg catgaaatcc
    34501 tcgataaaga tttcctccac caaaatgaag ccacactgga tgtttctgac cttcttgttt
    34561 gaccgcttcg acattaaaat acggtagttg aacttgcatt ttgttcaatc cttcgataga
    34621 atcttgccag tctttaatca aaaccatatt tacccctcca tactgatatg ttagtttccg
    34681 ttacaattgt agtataaaat cgaatgtaat cgctgtcaat aagaaaaaac taaaaatatt
    34741 cttttttcta ccatgtcaga attattgtgt ctttttttag taaaagcaca ccaatatata
    34801 ttttttagaa ctaacttaaa ttaaagctta atttctcttt gatcttattt gagtattttt
    34861 tgttttttta atagctagta tgttactttg aaatttgata ttatataacg agcatagaac
    34921 taaaatatca aattcaaaaa tgaaattttg agagggatta aatatgacaa ttaaaactag
    34981 tctccaaaat caagcttatc aatctattcg tcagcaaatt atctacgctg acttagaacc
    35041 tggaaaaaaa gtatcagaaa aaaaattgga agaaacgctt tctatcggtc gtactccaat
    35101 acgtgaagca ttgattcaac tccgtcatca aggacttgtt gatacaattc ctcaaagtgg
    35161 aaattatatc tcattaatcg acttagattt agcaaaaaat gctcgttttg tcagagaaca
    35221 tttagaacga caaattatgc tagagtgttg tgccaagatg aataatcaaa agaaacaagt
    35281 tttagaaact ataattgcag aacaagaaaa agctgttagt caaaagaatg ctcgtagttt
    35341 ttttcataat gataatttat ttcataaagc ttgttttgaa attgcagaac gggaagaagt
    35401 atggcaatgg ttggaagata ataatacgca cttggaacga tttcgttggt tacgagtaaa
    35461 aacggaagga ttaaaatggg aaaaaattat ggaacaacat catcaactat ttaaagcctt
    35521 gaccatgcaa gaccctgaag aagctaactt cttaacatcc ttgcatcttc atttaatgct
    35581 tgatgaacaa aatgttgtat tgactactca cccaaaatat tttaaaaata tataactgct
    35641 aaatttcttt tttttaattt atttacattt ctgattacta cagaaaacta actacttttt
    35701 ttatgtaaag ttctgtcaaa tatagtcact ttatattact ttctcttgag taaaaaaaca
    35761 tgtgaggtcg taaacaaaga ttgaactatc tacattcata ctctataatc tgtttatttg
    35821 cagactgata atagcaaaag aaaacttata atcaaataat atcaacattt gatcacataa
    35881 tttgagattg aatgctgatt tacctatgtt ttacgtaatg gtctttagtt atttttgctc
    35941 ataaaatgca taaaacacgc gtttttatgt gtgcttgaaa tttcttggct ctataatttc
    36001 taagactgat aaaaatattc tatcggttct ttttgcaaat aaaaaaacat atagaagctc
    36061 cattagattg aatcaccaca acacaatcaa aggagcattc cgtatgcttg attttcattc
    36121 tataaaacta cttttaggaa tcaaagaaaa tcacaacggt tcaaactaaa agcaagaata
    36181 tccgaagtac cattgtttca tcgatcatga gaaaagcatt cacctctgtc tgcagatgtt
    36241 cttatatcgc tctgtcagtc ctattaataa agtactgttt tgttttcaaa aaaactatta
    36301 cgagtacttt ttaagagaat gccttgcaaa tattcatcac acaccgttat ataagtcaaa
    36361 tagtttcttt cagtcgtcaa gaaaagattc tcctgtcctt ggcattatca gttaagtagc
    36421 aacttaattc tcggacttgt tctactctag caaaatttat tttcatcaac ttacgtccaa
    36481 atgttttttt gaataattac ttcatcgcgg gtctggaaga cttgcctcat tcgataagtc
    36541 actagctttt ggcgtccttc aggatttcta gtatacacca actgacattt gggcagcgac
    36601 gattgattta attcgtttag aaaaaggtta gcgatttgct tatttcggcc aaagcgttct
    36661 ggtaccgtca gaactatctt tttcccttcg attaaatacc gcggattctc cagcggtgct
    36721 aaaagctccc tcatagcttc agtaaaaagc tgtccttctc gatagccagc attctcaaga
    36781 tagcatctaa attcctgctg atcagccgct acgattactt ttctatcact aacttcacca
    36841 aggcgctgca acgtcgtttg aacgcatatg gccaagcttt ttttgcgggc acattccttt
    36901 tcccatagct tttgtcgcct acgaatttgg aaatattcca gcaagttacc tttaaatata
    36961 gctgaccaga agcttagagg ctctatattt tttactacta tcggcaatga agtttcttgt
    37021 ttttgcttta cctgatcgac tttcctaacg acgattggca tagataggtg ccagccatca
    37081 gctaaagcag tactccattg ttctttcaac cgacttcgtt cacaagaaaa atagaacatc
    37141 gactgagtat aagtttccat cgtggtggtc gtatattcca gtggtaaagc aaagcgatcg
    37201 ccaccactct caatttttgg tgaagattca aaggtcagac ctgaaatatt gcccaaacgc
    37261 cgtagcattc ttttcaaatc aggctgttca gacagaggaa ctcccggtaa tccaatcgct
    37321 aaatgccaaa tgttcgttac tttattttgt gcacctaggc gtaagccccg ccctcgtagt
    37381 tgttgtgttt gcacaaaagt ggaagcttga ttagccaaga tcaacagatt aattgcagga
    37441 caatcccagc cttctcctag caaactaacg gttcctacca aaatattcaa cttcttttcg
    37501 gttaataggc gagtaactgc ttgcatcaat gcttggcggt ttttgtcatc caccgtcaaa
    37561 taaaaataac ccgacatata aggatgttca ttcatacccg tcgctagttc tgccagctca
    37621 acagttacta gcattttcgg taacagtaag aactctccac agatggcacc gatttgctga
    37681 gtgttaccta atagacgaac aacttcatca aacaaaggaa ccacgccata agctaaggta
    37741 ccatcactgc ctgctaatac ctccttgcaa atacgatcca tcaaaatgac cgctgtcagt
    37801 tcttgcttca gctgaacttc ttcttttaac aaaatttgac aaactgcttg ctttttctca
    37861 ggaatcgcct gactaccaac agtgtctgga tactctggaa acaaatgcag cgactcatca
    37921 aacaatcatc cagctttttt caattgataa aaaataggca attccggttg atcttcaaac
    37981 atctgctgat aaatagtgat cagctctttt ttacctaaag atggaactga agctttttta
    38041 ccttgctgct tagaactgcc taatagctgc caatgttcgg gtatcaattc atagttatga
    38101 tgattcaagt aaattaatgc actagtgaac agctcaaaat tttcataaat ttccttcgaa
    38161 tgctcaagcg gatgctgaac aaattctaaa gaaagcaaat aatccaccgt ctcttggctt
    38221 tccattaacg cttccaccag ctcattttgt ttctctaaaa aggtaaaata atgctgagta
    38281 gtttcctgat tagccggaat caaatataga aaatcctgat gaggacatag aacctcctcc
    38341 tttaccaact cgggaatgct aatttcttcg tcaatcaggc cattttaact caatatattg
    38401 cttccactcc gtctgagagc tatcaatcgg aggcgtggct gtcaaggaaa tcgtcaacag
    38461 attcggttgt tttttcttca agtcaatcaa caattcaccc cagctttttt ttaaatgatg
    38521 agcttcatca aaaaactaac agctccagct gatcaccata agttggctcc tccttcagtc
    38581 ggttgtaaat ggtctgatac gtttccactg taatatgact aggatgttca atcgccccag
    38641 aaagatagca actatctgct ggatttacta agaaatattc ctcgattgtt gttaaccact
    38701 gtttcttcaa tattagggaa ggaaccaaaa ttaaggtttt acaatccaaa cgtcggatca
    38761 tctcaattcc taaaaaggtc ttgccagccc ctggtggagc cactagatgg actgaattgt
    38821 tatttaaata agtagcaaag ttagtcaata tacgcttctg atagctacgc cactgaattt
    38881 tttcatcaga tagccacatc ccaggtcccc ctctcacgta ttttctctat cataatctaa
    38941 atgaatacag caaaaaaaag atttcttcaa gaatatgtgt ttttatagta atattttact
    39001 tactctaaac tcaaatgaaa aggtaaaaaa actggtagta caatctttta aattctcatt
    39061 gttttgtacg ttctttacta tcaaaaaaac aaaaaaaacg cgtttctacg cgtgaccaaa
    39121 atttttagct ctataatttc taagactgat agaaaatcat tctgtttttg aagtcttttt
    39181 tcataaatac tacttgattt attgtgtata ttgtataaaa gattgttcat aattattttt
    39241 tataattaaa atttggtatt gatgaccagc gattccgtat ataatgcgca atcaaattaa
    39301 gctttctgtc tctggtaaat attcctgttc tgttttcttg tactcgtaag atttcttcac
    39361 ttatcgcaaa attcataaaa ttccacactt tttcataaac aaaatgtaca catttatcta
    39421 ttacttcata atagtctact tgatattctt ctgtaaacat ggttgctact gtatctgcac
    39481 catatttgat aaacatttgg gctactccct taaattgaga aaagcccatt agtttatcta
    39541 ctaatacttc taaaatttct tgctttcttc tttaacatcg tcccgtaagg aatcatgtag
    39601 tatgctggag gaagttcatt ttctaaataa ttgtatcgct tcaattgctc aaaggctatt
    39661 ctctacaatt aattacacca attactttat cttcacttat ataagatttc taaacatttt
    39721 tcgtcaaatg aagagaaaat atttttttaa atgatcttgc tcaagatttg tcaacgaatc
    39781 tttgaattcg ttttgaagtt ctgaggaagg tatcatttaa aaactcaatc tgactttcat
    39841 catttcgttt actcccattt tgatcacctt ctaccaacaa tttattaaag tgattatact
    39901 aggtttttat ttttttaatt cacaattgtt tattcacttc atgaacaatt aaacacaaaa
    39961 tcagtattag gattaaactt tcacccatta atacactaac gaaaaaatt
//
LOCUS       DARBDV010000013        37213 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000013 DARBDV010000000
VERSION     DARBDV010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.013"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            68..751
                     /locus_tag="SCM56_000708"
     CDS             68..751
                     /locus_tag="SCM56_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HEG1109530.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            753..1589
                     /locus_tag="SCM56_000709"
     CDS             753..1589
                     /locus_tag="SCM56_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEG1109531.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            1600..3354
                     /locus_tag="SCM56_000710"
     CDS             1600..3354
                     /locus_tag="SCM56_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1109532.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(3630..3971)
                     /locus_tag="SCM56_000711"
     CDS             complement(3630..3971)
                     /locus_tag="SCM56_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEG1109533.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(4039..6210)
                     /locus_tag="SCM56_000712"
     CDS             complement(4039..6210)
                     /locus_tag="SCM56_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEG1109534.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            6379..7278
                     /locus_tag="SCM56_000713"
     CDS             6379..7278
                     /locus_tag="SCM56_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEG1109535.1"
                     /translation="MVQLVCNCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(7349..8206)
                     /locus_tag="SCM56_000714"
     CDS             complement(7349..8206)
                     /locus_tag="SCM56_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG1109536.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(8191..10893)
                     /locus_tag="SCM56_000715"
     CDS             complement(8191..10893)
                     /locus_tag="SCM56_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEG1109537.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(10906..12195)
                     /locus_tag="SCM56_000716"
     CDS             complement(10906..12195)
                     /locus_tag="SCM56_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEG1109538.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            12345..13391
                     /locus_tag="SCM56_000717"
     CDS             12345..13391
                     /locus_tag="SCM56_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1109539.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            13393..15546
                     /locus_tag="SCM56_000718"
     CDS             13393..15546
                     /locus_tag="SCM56_000718"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEG1109540.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(16019..17470)
                     /locus_tag="SCM56_000719"
     CDS             complement(16019..17470)
                     /locus_tag="SCM56_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1109541.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(17467..19191)
                     /locus_tag="SCM56_000720"
     CDS             complement(17467..19191)
                     /locus_tag="SCM56_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEG1109542.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(19184..19798)
                     /locus_tag="SCM56_000721"
     CDS             complement(19184..19798)
                     /locus_tag="SCM56_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEG1109543.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            complement(20143..21600)
                     /locus_tag="SCM56_000722"
     CDS             complement(20143..21600)
                     /locus_tag="SCM56_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1109544.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            complement(21641..22585)
                     /locus_tag="SCM56_000723"
     CDS             complement(21641..22585)
                     /locus_tag="SCM56_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1109545.1"
                     /translation="MHYSKEEKNEKKKVERVQVRSFNKTANFFSNIMIALFAISCILP
                     FIFVIAISFTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGT
                     LINSTATSLYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWA
                     LILPMAVSPFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATIS
                     LFAALGYWNDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVP
                     GESARMAIVVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            complement(22572..23519)
                     /locus_tag="SCM56_000724"
     CDS             complement(22572..23519)
                     /locus_tag="SCM56_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1109546.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            complement(23998..24717)
                     /locus_tag="SCM56_000725"
     CDS             complement(23998..24717)
                     /locus_tag="SCM56_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEG1109547.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(24766..26412)
                     /locus_tag="SCM56_000726"
     CDS             complement(24766..26412)
                     /locus_tag="SCM56_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEG1109548.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            26591..27181
                     /locus_tag="SCM56_000727"
     CDS             26591..27181
                     /locus_tag="SCM56_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG1109549.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            27273..28778
                     /locus_tag="SCM56_000728"
     CDS             27273..28778
                     /locus_tag="SCM56_000728"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEG1109550.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(28862..30214)
                     /gene="celB"
                     /locus_tag="SCM56_000729"
     CDS             complement(28862..30214)
                     /gene="celB"
                     /locus_tag="SCM56_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEG1109551.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(30214..30732)
                     /locus_tag="SCM56_000730"
     CDS             complement(30214..30732)
                     /locus_tag="SCM56_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109552.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(30748..31074)
                     /locus_tag="SCM56_000731"
     CDS             complement(30748..31074)
                     /locus_tag="SCM56_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HEG1109553.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(31087..33066)
                     /locus_tag="SCM56_000732"
     CDS             complement(31087..33066)
                     /locus_tag="SCM56_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEG1109554.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(33087..33401)
                     /locus_tag="SCM56_000733"
     CDS             complement(33087..33401)
                     /locus_tag="SCM56_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HEG1109555.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            33569..33862
                     /locus_tag="SCM56_000734"
     CDS             33569..33862
                     /locus_tag="SCM56_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEG1109556.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(33940..35052)
                     /locus_tag="SCM56_000735"
     CDS             complement(33940..35052)
                     /locus_tag="SCM56_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEG1109557.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            35439..35618
                     /locus_tag="SCM56_000736"
     CDS             35439..35618
                     /locus_tag="SCM56_000736"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109558.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            35812..35982
                     /locus_tag="SCM56_000737"
     CDS             35812..35982
                     /locus_tag="SCM56_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1109559.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(36406..37155)
                     /locus_tag="SCM56_000738"
     CDS             complement(36406..37155)
                     /locus_tag="SCM56_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG1109560.1"
                     /translation="MVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQ
                     SGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENR
                     LTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQL
                     VETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPK
                     TGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT        11034 a         7633 c         6542 g        12004 t
ORIGIN      
        1 ttgctccgtc ttcttctctt gctgttctgc ttcattgcct ataaaccttt atgaaagaag
       61 agataacatg cctactatcc atattgccgg tgattctact gcggcagaaa aagatgaaaa
      121 caagcgtccc gagacaggtt ggggagaaaa aatctctgct tatttttcac ccgagatcca
      181 gatcaataac caagcaaaga atggacgcag caccaaatca tttatccagg aaggaaggtt
      241 agcccgtcta gcttcacagt ttcagccgaa tgatttctta tttgtacaat ttggacataa
      301 cgaccagaaa atccaagaag aaagaggtac gcttcccttc ggtagctatt taaaaaatct
      361 gcagatattt tttgaatgcg caaaaaaagc gaacgtccaa atggtcttac tgacatctgt
      421 gacgcgacga gattacttag aaaacggaac attgaatcca gatatacttg gagactatcc
      481 aaaagcgatg cgtgcatttg ctgagaaaca ccatattcct cttttagatg tgttctcaag
      541 atcacaagaa ttgttccaga ccttctcaaa agaagagaca aaaaaattct acctgcatct
      601 tatgcctgat acttgtaaaa attacccaga aggactaaaa gacaataccc actttagtcc
      661 agaaggcgct gataaagttg cccgaataat tgttgaattg ataaaagaat cccgtctgcc
      721 tcttgctaat tatttacaaa aaggagtttg agatgtttcc attaaattta ggcatccgtg
      781 cccacgattt agatgttcga aatcgtgaag aaatcgttca aaaagtatca cagcttcact
      841 tgtcccatat ccaatttgca ccacaaaaag catttcctga acaacttaaa ttaactgaca
      901 tgacacttgg gagtgctgcc tattttggtg aatacttcca aaaaaacaat atcgaacttt
      961 ccattttagg atgctatatc aacctatcca gcaaaaatca agaagttcgt gcacaagcat
     1021 tggcaacttt caaacagcaa attgctctgt gctctgccta tcaagccaag atgattgcta
     1081 cggaaaccgg tagtgtctct gtcggttaca caaaagaaaa ttttactgaa gaagcttatc
     1141 agatcgccag aaattctatc attgaattag tcgctcatgc agaaaatttt ggcgtaactg
     1201 ttgctgttga agcagggatc aatcatccaa tctataacta tcagttagct aaacgtttaa
     1261 tcgatgaagt agcttcacca aatttaaaaa tcattttaga ttgtgcgaat ttgatgcata
     1321 aagaaaatca tgctgatcag gaaaaagtga tccgaggtgc cttggacaat ttacatggtt
     1381 atattacagc gcttcatctc aaagactata tcatggaaaa tggaaagatc cgcatggtac
     1441 ctgttggcaa gggctgttta gatttccgcc cggtactgca ttatatcaaa tatgagcgtc
     1501 ctctaatgta cgccactctt gaagctactc ctgagattta tgttcctgaa gcaatagaac
     1561 acttgcagtt tatttaccaa gattgttaag gagaagatta tggaggaacg aatacagaaa
     1621 agttatcaaa aatatcatgc aaatgctttt cagacattgc tgcatcttta tcagggaaat
     1681 tattggcgtt ttgccatgtc aggactcttt tttattatca aacatcttcc atcttggtta
     1741 atgccgatcg ctattgccaa tgtaatcaat gcagcttcat cgaaagattc ttctcagttg
     1801 caggccattt atttcaacgc ttttttgatg gcttgtctga ttggtcaaaa tatattgacg
     1861 aattattttc atgtaaaata ccatagcact tcgatccgtc aagtagaagc tggactaaga
     1921 gcgaccctta tcaagaaaat ccaagaacta tctattcctt atcaaaaaga attgcaatct
     1981 ggacgaatcc agtcaaagat catccgtgat gtagaagcta tccaaacgtt gtcctctcaa
     2041 gtatttgtca gcggattaaa tatcgctgtc aatttattcg tagctttagg tatcacgctt
     2101 tataaaagtc ctgttgtttt catgtttttc ttattgactg ttcctctcgc cgcattcgtt
     2161 actctctttt tccgtaaaag gatcagacag tccaactcag acttcaggca agaaatggaa
     2221 gagacttctg caaaagtgat cgaagtgatc gaattagcac cgattgctcg agctcatggg
     2281 ttagaaaaac acgaaacaac tcgcttaaat aagcatttta ctcaaatcta tgaaaaagga
     2341 ttccacttag atatcctgca atctgttttc ggatcagtca gctgggctag ttttcagata
     2401 tttcagctga tttgtttgat tttcactggg atattagctt tacaagggcg catccaaccc
     2461 ggcgatgtcg tgatgtatca gacgtatttc acgacgatcg tcaattccgt ttctggaatg
     2521 atcacgctga ttcctacgat ttcaaaaggc atggaatcta tcacttctat tggcgaactg
     2581 ttgattgctg gcgatgtaga aacttatcac ggtaagaaaa aaatccaaca tttggatgga
     2641 aattttcgtt ttgaggatgt ttcttttctt tatccggact cactcaaacc gattatcgat
     2701 catttttctc tagaggtgaa agctggagaa acaattgctt ttgttgggcc atctggctct
     2761 ggaaaatcta cactccttaa cttattgatt ggatttatcc agccgacttc aggaaaaatt
     2821 tttctagatg atcagccttt ggatgaactg gatatgcgaa gtgtgcgaaa ttatttagcc
     2881 gttgttcctc aaaccacttt gttgttctcc gcttcgatca aagagaacat cacttatggc
     2941 ttgaaaaacg tatcaaaaga acgtttagat gaagtcatcg aagctgcgca actttcttca
     3001 ctgattgacc agcttcctga tggtctggat accttagtag gcgaacatgg caacaagctg
     3061 tctggtggcc aaaagcaatg tatctccatt gcacgagcgt tgatacgtca gccaaaaatc
     3121 attttgctag atgaagcaac ctcagcttta gataaccagt cagagaaaaa aatccagcaa
     3181 gccctgaatt acctgacaga aacacccact acttttattg tggctcatcg gctttcaacg
     3241 atcaaagagg cagataaaat cgttgtgatc gatgaaggaa aaatcgttga aataggtact
     3301 tacgaaaaac tgttagctca aaaaggctat ttctaccgct tgaaaaatga atgacaaaaa
     3361 ataggagcta tgacaagact tttgtcgtag ctcctatttc tgaataaacg gtgttctcct
     3421 gccaactcct cggtcacaag tcacaatagt catcaaacat gagaagctgt tttttgaaat
     3481 tgttccttgt aactcagagt taaacggtag gctcacgacc tcttggtaaa tggtgttcaa
     3541 gagacagcct atcggcaata agaagcaatt ttgtcatcgc tcaaggccga acgtctcttt
     3601 cacaccctct gttgtttcat aaaatagttt tattcgctgt aaaggtcgcg aactggtgtc
     3661 atgatgatac ggttcaagtc attgatgatt gtactgaagc cttgctcttt ttgcatcaag
     3721 tcattgatta cttcttctga ttggattttc acagccattt cttgtgcttt cattgcatct
     3781 tcgtcgctga actcttgtcc actcatttgt ttttcttgta attcgatttg catcgcttgg
     3841 aagtctttga acagtttgtg tgcttcttcg tttgcttgta ctttctcgta cgcttctttc
     3901 aacgctttga attctggtaa ttcacggatc tcacgttcga tttgattcgc gctatcgtag
     3961 atgtttgaca tgttatgccc tcttttcttt tagtagttac tctctattgt aacatggggt
     4021 tattcgtttt caaaggggct actctccttg atatttccgc cagaggtctt tagccgcttc
     4081 accaattccc tgaacgcctt ctttcaccat atcgccgaag tttttcagcc cttcttttgc
     4141 ttgttcacca tattgattca agttatcttg ccaatttcca gactctccat tagaatttcc
     4201 tgtgtttcca acttgatctg cagctaccac ttgtcctcct gtggcatagg catctgctac
     4261 actaaatcct gtttttgaag tatacggaag gatgccttct gccgctgatt tgaagacttt
     4321 cccgacaaca tttccacttg tcccttccaa atagtgcagc ttgctggatt cttcgtaacc
     4381 catccatgtc gaaatcacta cgtctggcgt gtaaccgatc atccattggt catttgcttt
     4441 tgtggcatcg aaattagttt ctgtggttcc tgttttccct gctatcgtat aacctgcagg
     4501 attcgcagca gccgctgttc catttgaaaa tgtaccaaga agcatgctgg tcatttcatc
     4561 ggctgtttcc tttgatatga cctgttttgg ttttttgttt gtgttatcga cgatcaccgc
     4621 tccagtcgaa tcaacgattt tcgtgataag atgcggcgta tacatctgac catcattggc
     4681 aaatgcactg taagcacccg ccatgatcaa tggagatact cctttttcca gtccaccaag
     4741 ggctaagcca taatacttat cagattttgc taaaggcagg ccgaattctt ctgctttatc
     4801 atatcctttt tgtaaaccga tctcatgcaa aagccataca gcaggcaaat tcaaactttg
     4861 tgcaacagct tgatacatcg gtacttctcc ttggtatgtc ccgtcaaaat tatgcgcatc
     4921 gtaataactt tgcggttcgt ctttcaagat actggaaggc ttgtatccag cttccagtgc
     4981 tggtgtatat acgcttaatg gtttgatcgt tgatcctggt gaacgacgca tctgtgtagc
     5041 aaagctgaat cctctaaaga catgatctcc tcttcgtcct actagtgctt gtacaccacc
     5101 tgtattcgga tctaaagcta ctgaagcaga ttggaccatg gcaccatctg aagcatttgc
     5161 tgggaataat gcactgtttt gataagtagc ttctaattgc ttttgataat cctgatctaa
     5221 agctgtatag attttatagc ctttattcat gacatcttct tcatcaagac catactcgtt
     5281 aactgcttca tcgatcactg catcaaaata ataaggataa cgataaccag aatctgaatt
     5341 atcgtatgtg tcattaagat acgcgctgat atctgtctgg ctttctgctt gttcttgttc
     5401 ttttgtgatt tttccgtttt cttccattac acttagtact gtgtttttcc gatcattcgc
     5461 attttttggg tgatcgatcg gattataaat accaggacct ttcagcattc ctgccaaagt
     5521 agcagcctca gacagcgtaa cattcgttgc atctactcca aaatactttc tggaagcgtc
     5581 ttggacaccc cataccccgt tgccaaaata ggcattgtta agatacatgg tcaagatttc
     5641 ttctttgctg tacttctttt cgatctcgat tgccaaaaat aattccttcg cttttcgatc
     5701 aaacgtttgg tctaatgtca gatacgcatt tttggcaagc tgctgagtga tcgtacttcc
     5761 tccgccccct gatgttccgc cagacaagac cattcggact gctgcacggg caatcccttt
     5821 gatatcaaac ccgtgatgct cgtaaaaatt cctatcttct gtcgagatta ctgcatcttg
     5881 gatataggga gaaatcgcgt ttaattctac atatgttcct ttttgggcgt acagtgtgcc
     5941 agcttcttcg tttttcttat cataaatgac tgtcgattgg cttaatcctg attttaacgt
     6001 ttcgacattt gcttgttttg ctaaaataaa gaggtagata cttgtcacta agacacagat
     6061 caaaccaatc agcaaaatca atttgttcac atgatatttt ttccagatgc gctttcgcca
     6121 tcgatggaat tgtccgagat atggtctgat ccaacgccaa aaactgatca aagcagcttt
     6181 tatcttttct aaaatgcttt gaaaatccat ttgcttcttc tcctgtctat ttgattcaat
     6241 cgtcttattt taccatagtg aaagagtgtt ttcttcctgc ttaagttgta gttcaatatg
     6301 tttttttacg ttagtgcaag tagagtaaca taagaatgaa cagaaaaatg taagtttttt
     6361 cttaaatatg ttttaaaaat ggtacaatta gtctgcaatt gcactgaaag gagtacactt
     6421 gtgaaaatca cgatcacttt acctatgaac catcctgaaa caacaattcg cgacttgctt
     6481 gaaaaggaat ggttagttcc tagaaaggtt cgacactttt tgcgtatacg taaaaacgtt
     6541 tggatcaatc aacaacctgc tttgttccat catgaagtaa agggcaatga ccagatcaca
     6601 ttgttattcg aagaaacaga ttataattat caagaagttc aattaggaga tgcgaagcac
     6661 atccgtgttt tatatgaaga tgagcatttg attatagtgg ataagcctgc aggaatgaag
     6721 actcatccaa atgaaccgac agaaaacagt accttactca atcatttagc tgcttatctc
     6781 gacactaaac agcaacgacc ttacgttgtc caccgcttgg acaaagaaac aagcggtgcc
     6841 gttttatttg caaaaaatcc atttgtcttg ccgatacttg gaagaatgtt ggaacaaaaa
     6901 atgatctatc ggagatatca agccatcgta tggggagaaa ttgccaaaga tatgaccatc
     6961 accaataaaa ttggacgaga tcgccatgat cggagaaaaa gggtcgtaga tgataaaaat
     7021 ggtcagagcg ccttgacaca tgtcactgtc aacaaagtac tttctgaaca aacacaagtc
     7081 tattgtgttt tggaaaccgg acgtacacac cagatcagag ttcatttagc tcataaggga
     7141 catccgatcg ttggagaccc tctctaccaa aatagaacag ctgaacgact gatgctccat
     7201 gcattagaac tgcacatgat ccatccattt actcaagaaa aaatagtcgg taaagcttta
     7261 cctggattat gggaatagaa ataaaaaaga acagagtctt atcagcagat aatgataaga
     7321 ctctgttctt tttgtgtaat ttgtctgctt acttttgatg ttcgtgttct gctaacagca
     7381 aactaccgat aatcccactt tcattcggca gtccccaacg aacgatatat tcacttactt
     7441 gaggtgtttc catataacca cccatcaatt caacaaattg ctgacggatt ttttgaagga
     7501 gatgatcctg attcattacc ccaccgccta aaataatctt ttcaggtgct aacgtcaacg
     7561 tatagttgat cagtgcttga gcaatataat aagcctgaat agcccaaaca ggatgatctt
     7621 gcggcagatc ttgccctttg atccctgtgc gtgcctcaat agacggtcct gctgctaatc
     7681 cttctaaaca atctttatga taaggacaag taccttcata atggtcatct ggatggcgtt
     7741 tcacccaaat atgtcccatt tcaggatgtg cgatccctga aaaaatttct tcattgatca
     7801 cgacacctgc accgattcct gttcccacgg taaggtaaat acaactattt tttccttgtg
     7861 ctgctccttg cttcaattcc ccatatgcag cagcattgac atccgttgtc caagcagcag
     7921 gaatattgta gcgttccttg attgtgccaa gaaaatcgaa ttgtgcccac ccttttttgg
     7981 gagttgccaa aacgtatccg taattatctt ttgttttgtc gataccaatc gggccgaatg
     8041 agccaatccc gattcccttc aattcataac gatcaaaaaa ttcaaatacc tgatccaatg
     8101 tttcttctgg tgttgtggta gggatactta atttttctaa aatttctcca ccatctgaaa
     8161 ccgcacacac aaattttgtt ccgcctgctt ctattccgcc atacattcgt tttcctcctc
     8221 gaatccgatt gtccgaatct cataaggttg gattattggg acaaccgctt gcttcgttgg
     8281 ttcttcaagc agattcaata tgacagtttt tccattgtct ttagttattt ccaatttttc
     8341 taaatgacta gacatattga aacctcttac tactacttta tcactaaact tgctgcgttt
     8401 caaagctgta attgcaaacg tttcactttt tatcgttaaa tattgttgtt ttgaaatcaa
     8461 tgttccttca tgatgtttga tttgttgagt ggaaaaagga atctgtgctg cgtatgcatg
     8521 caaataagta gagaattttt cttctggtcc atgtagttca atgctgtaat tgaaccgatg
     8581 ctcacctaga cactgtgctt ctggtgttgg aaaatatccc caatcgccta gttctcccac
     8641 actgcgaagt agcgtcactg cgatttgtcc gtcttctgtt acttcatatt cattcaaacc
     8701 aaagtttccg actgttacac cgtactcttc atcatgaata ttgacaaatg cttgctgatg
     8761 ttgcggattc gtggggtttt cccaactttt agacacatgg tttgggcgct tcaccacttc
     8821 aaaaatacta tctgcttcgt gatgttccac atgtaacttt gtaggaaaca agacacgtaa
     8881 gcgatgatct ttcatctgat tgtcgagagt cgtttcaaag tcgatttttt tactatcttt
     8941 tctgattgtc atgattgttt ttatttgaag gattcgtttt tctttggatc gttctgcttt
     9001 tcgataacga aattccacaa ccatctgctg ttctttttct aaacgctcgt ctgcagaaac
     9061 agggatttcc atttcctgta gaatacttat ttttgctatt tccggtgtat ctactaatac
     9121 ttttacttca gagttttctt tgttactgga taggattggc tgatcacaga aaggttgttt
     9181 aaagatatac tcatttccta tatcgccgat atcttcaaaa atatgcagat tattcaactt
     9241 tcgtttgttt ttgcgatctt ccattgtaat ggtaccattt ttttcaatcg tcaaatgtag
     9301 agggccgttt tcgatgattc gtccactctg atgaatcatt gtttcctttt cttctgcttt
     9361 cgcttcccct tctacaagcg cgaatgtttc ccaagaaaat gctggcatgt tttctaatgg
     9421 taaagtcact ttgatcattc ttgccatata tggcacacgg aaacggtctt tcggcaaatc
     9481 ataaccaaat tggacagttt cttcagataa aatagctggt atagtggccc cactttttgt
     9541 ttctacatga tacttacgtt taggcaaatt ttctaactct tgatatagtt gttcaggtat
     9601 tccttcagcg aatctttttc tttctaattc gatggttatt tctgcttcac cagttttatc
     9661 cattcctgca gtatttacaa tgacaaatgg aaagctgtct tttgggaatc cggtcgtatc
     9721 gattgctgcg gtcagctgtt ctaaggaatc ttgtgcaaga tactttccaa cttcatctgc
     9781 tttttcaaaa cgaggtatca tttcccgatg gacagaatcg actgaacaac cgcaaatact
     9841 gtcgtgagga tgattttgca ttaatgtctt ccaagcataa tctaattgat catggggata
     9901 gtttccacta acttcataag ccatcgtcgc aagtggttct gtaatatttt ctagctgtcg
     9961 ctggaccttg gtattccatt gttttaagta aacccgagca gatgctgtat tggctaaagt
    10021 ataccagcca tccgtttctt gacttgtcaa ttctccttct actgagccta agtcttctgg
    10081 cacatctttt tgaactgctt ccagatagtc agtaaaatta gaatgaataa actcgtaatc
    10141 tggaaacagt tcatttgcga gatggattgc ttttgatata tctttttgta ccggttgatg
    10201 atcaacacca ttcatcatca ataagtggtt cgtagaggca tattgttctg catccgctaa
    10261 tttttgcttc caaaaggcaa gagctgcttc tttttctact ggaatctcat tcccgttgct
    10321 ataccaattg gcaaatagca gaccaaagat tgctgtttga tcagggcctt tccaccacat
    10381 ctctgagtat tgggaagaat agttttctgc ttccaaaact tgattatcaa agccaattgg
    10441 ttttacacca cggccaaacg ctgctgctga aataccagct tgtttcatta attgaggcgt
    10501 ctgtcccata tttccaaatg tatcgggaaa atagcctaac atgacagggg ttccccattt
    10561 acggctttct tccatgccaa tcagcatatt tctcacgttc gattccgagc tgataagaaa
    10621 atcatcttgc aagatataaa atggtccaat tctcaatttt ccttcattga ttgcttgttg
    10681 gattgcctgt cttttttctg gccttacttg aagatagtca tctaaaataa tcgtttgccc
    10741 atccaaatga aaactgttga aggacggatc aacttcaaat agctctagta gatcgtctat
    10801 caattcaatc agtcgcatgt gatgctgttc gtaagccatg taccattcac gatcccagtg
    10861 gctatgagaa atgatatata ctttcttttt catttacgat acctcctact tttctactct
    10921 aatatcaaag taatccatga ctaattcgca aaacatcata tttgcccaag aaaaccattc
    10981 tctcgtataa ttttcaggat tgttcacatc gaatccttcg tgcataaggt gtgttcctgc
    11041 gtcagttgct acaagtaaat tcaatatccg ctctttttca gatttgtctg acgttgtcat
    11101 tccttccata gctaatgcaa tcggccaaac gtatttttct ggcgtatggg aggatccaat
    11161 tccttttgcg tactttcctt catagaaata tggattttct ttactcaaca atgttcttct
    11221 tgtttgcaga taatcttcct cttgttcaga acaatatccc aagtaaggtg cggcgatcag
    11281 attgggcaca ttactgtcat ccatgactgt ggcgtttccc attccatcta cttcatatgc
    11341 ataaatcgtt tgcccttttt gattaaccgt tcgtccgtga ttctgaatcc cctcttggat
    11401 ctctttcttc aatttctgtg tttgctgtac aatttcttta tcatcgagta tcgtttgaaa
    11461 aatttcttcc acataaccta acacgaccac cgcaaacata ttggatggta ccagatatcc
    11521 ataacggcaa gcatcatcgc taggacggaa accagaccat gtcattcctg taaaagctgt
    11581 ttttgcacct cgcccgtcat tgatcagcgt atcttctttt cttgtcgtgt ctcgtacaaa
    11641 tgtataagga gagtgttcat gatcttgctc tgttttgaac acgtccaaga tttttttgat
    11701 accttcaata aaagattgat tgaattgatc agtttttcct gtatttttgt aaagaagata
    11761 ggccaactgt attgggtagc ataaagaatc aatttcatat tttctttccc agatccaatc
    11821 gttcattttt gtatgatctg tttggtggcc agcaccgtta gcttcttcgt tgaaggcatt
    11881 cgcatatgga tcgatattga tataaaaaaa ttgctttttg actaacccgc tgatcatgtc
    11941 agctaattcc tcatcttctt ttgcaatcac taaataagga cgaacttgtg cagtagagtc
    12001 tcttaaccac atcgctggaa tgtctcccgt caataaaaag gtggttccgt cattttgctt
    12061 tttgactgtt gtcaacagag tattggcaaa tgctgctgta aaattctttg cccagtccgc
    12121 atggatatcc ccacattttt gtgtgatatc ttccataaat ttctgcaccg attttggtat
    12181 atctttataa accactaata aaaccttctt tctatttgat atatctttaa agattatact
    12241 tcatacagcc aattatgtct acgctttcaa aaaatatctt tttatttact ttttcatcca
    12301 ttatggtatt atttgaatat gatatatcat taagaggtgt ttatatgact caaccattgt
    12361 accagacaat atttaatgat ttgcaaacgg ctattttaac tggtgaatta cctgtagaca
    12421 gccaactacc aactgaaaaa gaactttcac gtacctataa agtaagcaga atcacttcca
    12481 aacgtgcatt gactgaatta gagcaattag gacttattta tcgcgttcgc gggaaaggca
    12541 gtttcgttaa atccgcttct ccggaacaga ttcttgcttc tcctacaaaa acacgacgga
    12601 ttctgtttct tcttccatat gtcgctgatt tgtctgttgg tgattttagc aagggattga
    12661 accctgtgat gcaagataaa cagattgatg tcatgatgac gacgcttgac tttctacaaa
    12721 ataaaacagc aaaagagcta atcaatgaat ttgacggatt gatttattat gctcttgata
    12781 cagaacaaca tcttgactta ctttttgagc tttcattgaa agaattccct gtcgtcatat
    12841 tagataaaaa aatctatgag ttaccttttc ctaccgttct ttccgataat ttccaaggcg
    12901 gtgcgcttgc tgcctcttat ttgatcgaac agggacattc aaaaatcgct tatttgtttg
    12961 gtacgcagac tcatccacaa tccgttcgac agcgttacct tggttacctt gaagcattgg
    13021 acaaagctgg tttgactttc cacacttcac tggatgacaa aagcgcgaca ttagatgaat
    13081 tgctttctta tatcactaag tatgaagtaa ctgcttttgt ttgtgaaaac gatgttgtcg
    13141 ctatccagac catgaatctc ttgagacagc atggttatca tatccctgaa gatttttcaa
    13201 ttatcggatt tgatgatatc caagcagcag ctcttgttga cccgccactg acaaccatcg
    13261 cacaagattt tgaacagtta ggtaagcttg cgggagatgc tttggtcact tggttagaga
    13321 caaaagatcg accatccgat agtaaatttc cagtttcatt catcaaaaga caatcaacaa
    13381 aggagaaata aaatgaatat cggtcaaata gatactagac atgcaacagc gaatcagcca
    13441 aatttctcta atgggaactg ccagccttat actggcgttc cttttgggat gaattatttt
    13501 gcaccgcaaa ctacggatca aaacggcagc tggtggtttc atcctgatga ccgcgttttt
    13561 caaggctacc gtcttaccca tcaaccaagt ccttggatgg gtgatttcag tcatatgctg
    13621 tttacgccga tcaatggaaa actacaggag aacacccttt tccatgctca aagttcttac
    13681 cgcccagaag aaagtatatt ttgcccgact catctgtcga tctgtcaatt acgttatggc
    13741 atctgttcta cattgatacc tagcatgtat ggcggaatct tatctattga ttacagcaaa
    13801 aatgacagcg gtttactttt atcctttccg ggacgccatc agctctttgt tatagatcct
    13861 tacacagtgg aaggaaaaat cacacatttt gctggttcag aagatcctga tttttctttt
    13921 tattttatat tgagatttga atatccttta acaattgatg agttagctgt ttccaacaaa
    13981 aatagcgact cgatcccgtt gtattttgaa cagacaaaaa aacaaactat ccgatttggg
    14041 acatctttta tcagctcgga acaagcagct tataacctgg aactagaaca aaaccaaagc
    14101 aaagagactt accttgctca cagtcaagaa caatggcagt cctattttga tcgtatacag
    14161 ctcacacacc atgatcaaca acaattgcgg acgttttatc ataatttata tcgggtgttt
    14221 ttatttccac aaacatttta tgaaattgat cctagtgggc aaaaaatcca ctatgatacg
    14281 actagtcgat ctataaaacc tggtgttctc tatacaaata atgggttttg ggatacgtat
    14341 aaaacggttt atcctcttta ttctttgatt gctgtagaaa aatacgaaga aatgctggaa
    14401 ggttttttga acagctatag agaaagtggc tatttgccaa aatggctgtc tcctgatgaa
    14461 cgtggactta tgcctgggac attgatcgat gcggtcattg cagatgctgc tgtaaaagat
    14521 atccgacgag acttgatgcc tgaatttcta acggcaatgg aaaaaggtgc tacggttcaa
    14581 agcaaacatc caaattatgg acgccaagga acagaagatt acttgacata tggctatgtt
    14641 cctaatcact accacgaatc agtcaatcac acattagact atgcttatag cgatttttgt
    14701 attagccaag ttgccaaggt actagaagaa gaggaaatga cgaagaaata tcagcagcaa
    14761 tcattgaact atcaaaatat ctttgactat gagtcaggat ttatgcgttc aaaagataaa
    14821 gatggcaagt tcagagaacc attttcagct aatcgttggg gacaagatta tgcagaagga
    14881 agtgcttggc aaagcagctt tgctgtttat cacgattttt caggactgat cggaaaatat
    14941 ggtggcagtg agagatataa agaaaaactc attgagctgt gcaatcagcc cccaacgttt
    15001 gacccaggag gatatggttt tgagatccac gaaatgagtg agatggccgc gatagaattt
    15061 ggacaattag ctatttcaaa ccagccaagt tttcactatc cttacttgtt cagttatata
    15121 ggacaacctg aaatggcaca gccactgctt aaacagttga tgactcagac cttcgacgat
    15181 tctccaatgg gttaccctgg agatgaggac aatggaagta tggccgcttg gtatatcttc
    15241 aatagtctgg gattttatcc tgtcacgcct ggaaccggtg aatatgttat cggtatgcca
    15301 ttaattgaca aggctgtttt gcttttgtca aatggaaagg aattgacgat tgaagcaacg
    15361 cctaatcggc cgcagcaaca atttatccat aaaatcaaac ggatggacag agaatatacg
    15421 aaattatatt ttacccatca agatttgctg aaaggcggca cgatctctta ccaattaggg
    15481 atcatccctc accctaagaa atatcaagca aaagatttgc ctttttcctt atcaaactat
    15541 aattaacaag aggttgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaagcaaa
    15601 aatagatcct catatttttc gtttggttgg tcttattacc gcagatgcaa gcttttgaac
    15661 accgtttatt gagaggttgt gaaatcagcg ttctgacctg agtattagag agacaaatgg
    15721 caaaatagtt cctcatattt tgccattttt tgagctaata ccgagggtca gctgattgaa
    15781 caccgtttag tggagaggtc gtgaaaaagc tgtttagttt cgagtattaa gaaaaatttt
    15841 gtgaaaatgg ctttccacat ttttgcataa atttggctta ataccgagaa actggctttt
    15901 gaactccgtt tatcgagagg tcatgaaaaa gctgccttgc ctcaagtaat aagaacagcc
    15961 caaaattaga tttctagaca attttttata agttatttca ttacattggc tttttctttt
    16021 atgattctcc actgttttct cggtactctt taggggatct gccgacgatt ttcttgaata
    16081 atttagagaa atacgtggtg ttgttgtagc caatctcact tgctatctca ttgatgtttt
    16141 gattactgtg caaaagcagt tgtttcgctt tttccatacg taaatggttc aaataagctg
    16201 aaaaactttt tttcgtttct tttttgaaca gctgtcctag atacatgaca tttaaatgca
    16261 ggttttctga cacttctttg agtgtaagtg gttcttgata atgctggtgc aggattacga
    16321 ttactttttc cacattgtca ctgtactttt tctgtctctg atgctttttg atgaatgaca
    16381 gaagtagctg ctgaagttct tgcaccgttt ttgcctgatg gattttttcg attccttgta
    16441 aatattcctc atcttccaga tcaatcaatt cccgttgaat ctccataaac agtaaaaaac
    16501 tggaatatcg tatatcttcc ggcatcatgg ctgctaactg aaactgttca aaaaaatcag
    16561 tgatcatttt ttgagcgact tccaattgtt tattttgcag cacgcgtctg aaacttgaaa
    16621 aatcgatggt ttgatctttt gtttctgctt gtactgcgta aaaaacatgc tcgtctttat
    16681 ctccataaaa ttgatgcaac tgtagattgt ctttcgcttg ttggaatgtt tcagggattt
    16741 tgtccaaaga atctgtctct tccccgatac tgatcagcca ttcctgatca ggaaagtttt
    16801 cttgaacaaa acaacagaag ccatcaactt catctgcctc caacaacaac gtgatcaata
    16861 cacggtctcc gtaatttcgt tgatagtaaa agatttgttg ccgttcttgc aaccatctat
    16921 tgataagagt atcatgagtt cttgccagtt gaatcaatac tatcctatgc tgcttctcac
    16981 ttatttgtcc tattatttct tcttcactta tttcatccaa ttcttcattc aaccattgac
    17041 tgaataacag ttcttgatag atttcttgtt gatttttttg ttcattttgc tgatctaacc
    17101 gtgttttgat tttcttgagg ctttcaacca attcgaactt attcacgggt ttcatcaaat
    17161 aattcacggc gcctaattgc atacctcctt tcaaataatc gaattcttgg tatcctgata
    17221 aaatcataaa ttcaaaatta tgacgttctt tttgagccgc ttcaataaat tccagtccat
    17281 ttatttctgg cattgtcaca tccgttacta ctaaatcaat ctgtcgctgt tcaagtacag
    17341 ctagtccttc tttggcattc tctgctgttg ctactatttg aaagcctaaa ctttgccaat
    17401 caattatctt ttttaaccca ttcaaaatca tgtattcatc atctaccaat aacacttgat
    17461 acacgctcat ccctccttga atgtaataga aacggtcagt ccacctgtct cattattctc
    17521 aagcgacata taataactgc tgccgaagct ggcacgtaaa cgttcattca cgttttgcag
    17581 accgatagaa ccgtgaagtt ccactttagg atgagacagt ttttcttcaa tctgttttag
    17641 ccgtttttca gtcatccctt ttccattatc tttgatgatg atccttaccc gtttgccttc
    17701 ctgcaagact ttgacactta gcgcattatc gaatcgagta aaatcaatac cgtgtttaaa
    17761 ataattttca actaatggct ggatcacaaa tttaggtact tcgatctttt ccaaatcagg
    17821 atcgatcata aaatgatagg ctactcgatt aggataacgc atttgataca aatagacata
    17881 tttttcgcaa aagtccagtt cttcttttaa agtgatcgtt ttctcttgat tcgtattgtt
    17941 tcgaagcaaa gcggaaaacg catacacaac atctgctaat tcttcacttc cttcgctgat
    18001 tgcatacatt cggatatatt ctaatgtatt atataaaaag tgtggattga tctgtgcttg
    18061 aagcgcccgc atatgtgcgt cttgctgttt gatttctaat ttgtagatgt cttctacaaa
    18121 ctgattgatg ctatccaaca tttcgttgat accgattgaa agttccctca gttcatattc
    18181 cgtgtttgtt atgtcgatcc gcgtctctaa attccctgct gctgtttgat ccatggcctg
    18241 catgacgata gatacttgct gtgaatatcg tttgaatgtt ttataaagaa agataagcag
    18301 tatattgtct aaaataatga taccaatgat cagcggggca agattttttc ccgtttcgat
    18361 ttttacactt tttttatcta gtacagctaa tatacgataa tcatcagaca gttcttggtg
    18421 ttgaacaaaa ttgttttttt ccagatcgtc aatcggcaat tgtgaatttt tcttcagact
    18481 ctctgtgatg atcatttctt gtctttcaaa cgtttcttta gctattttat tatcatggaa
    18541 tgtgtatagt cgatttgtcg ttccagaaat catgtataaa gagagtccat tgaatgacgt
    18601 tagattttct aaaatcatat ctagttcttt cttttgaaaa ccaataaaaa tacttcctga
    18661 ctcagctcct ccttgtgtga taggcaactt gatataaaag gcgtctagta attttggtgt
    18721 cccctttttt atttccccac ctttgttttc tctagaagat tcataatact ccggtagttg
    18781 attcaacacg atatatatcg cacttagttc ttcataattt gcgtagagat ctttgatttg
    18841 atttgggaaa gaaactaatt catcggtact gttttgctgt tcgtatacat acatcagata
    18901 ctgatctatg ggttttgtta agtaggtcgt cacattttcg atcttttcgg aattccctac
    18961 aagctggttt ttgatgatcc tactcaaggt tgttttatca ttcagcattc tgcttgtgct
    19021 ttgaacggca tcacttgtca tgttttctgc ctgtttttga ttttgttccc aagtatggat
    19081 actaaaaata acggtagcag tcatcgttac accgatcatg ataaccgcgt atttccgcaa
    19141 cagacgattt agtaaggttc cttttgaaaa tagaatccga aacttattca taactttcca
    19201 actgtttatc taattcttca cgccaatgtg ttgtcatcgt accattccaa accgtcaaaa
    19261 gaccaatgac accaaacagg atcaatcctt tgaattgcca agtcacgcct aaaattatac
    19321 cgctaccaat aattattttt agaaaagacg aaaagctgga aaaaaccgaa ataaaactta
    19381 gctttagcag attaggaaca gagatcgtat attcactatc taaaatcata ctgtattgat
    19441 aagtagcaaa taggcagagc aaggcaaaaa gtaaaataaa atcaatagct aaaaaaatga
    19501 gccctttgat ttgaacagat aaatataaat tgtaactcaa taaagcagta ccagataaaa
    19561 ataaactaaa tagcagattg cctcttaaaa aatttctttt atatatattc cagcctcttt
    19621 ttattgtgat ttctttatac tcgaatccgt acaaataaaa tagttcattg actgttttcc
    19681 atgccgcacc taatccaaag atgatcccgc cggcaaatgt atatatccag aaatacaagg
    19741 ttagttttat aatgacccag atccggataa ataactcttc caaaaccttc cctaacatat
    19801 cttctcctcc aaacattaag tttatacttt ctcattatac aaaaaaaggt tgggcaatca
    19861 gcaccttgac ctgattatta gagaaacaaa cggaaaaata gttcctcata ttttgtcgtt
    19921 ttgtgagcga ataccgaggg ttagctgatt gaacaccgtt taccaagagt ttgtgaaata
    19981 gccgcttagt ttcgagtagt aagaaaaatt ttgtgaatat ggctttccac atttttacat
    20041 atatttagct taataccgag aaactggctt ttgaacaccg tttattcgga acaagaggtt
    20101 gtgacacgtc ttttgtcaca acccctttgt ctttattcat tattattctt gtcggaactc
    20161 atcatattgt ttttgcattt ctttcaatac cttatcatag ccagccttgt ccaaagcgtc
    20221 tttcaatttc ggtaatgttt catctggatc aacagtccct gtattcaatc catctaaata
    20281 ttgattcatg acattagaaa tattacttaa ttctgttttg acactgtcag tgttgaagct
    20341 gaatcctaaa atcggtgaag tttctgcatc agcaatcgat tgatcacgtt tggcaatcat
    20401 gtcatccgta atagactctt gtgtatatag aatcttgttg tttcctgtat tccaagcaga
    20461 catatgtgta tttggctgat aaccatctag caatttgatt ttatcatctg aaccatcgac
    20521 tttttcccat gcttctccct cgatccccca aacaagccca ttcaataatt ctggatcact
    20581 gttaagcagc cctagaagtt ctactgattt ttctttattt tttgaagtat ttgatacgac
    20641 aaaatttgcc atttgtgctt gagctgtaga ttttagtgga acagtaattg cttttgagac
    20701 aagttcttgt tttgctgcat ttgtcaaaat tgtatcgcca taatcatacg gtccttgtgt
    20761 ttctccacgc attagccaag tatttccttc tagtggataa tccttattgc tcgttgcagc
    20821 gtcacttgga atgagtcctt gtttgtacca gctatgcatt gtacgtaaca gtccgatata
    20881 cgtttcatta tcgtattggt tgatgatttt cgaatcatct ccatccagat caatagcaaa
    20941 tggtaacgta ttccctaaag gataatcaaa gttcccttgg actttgaagc cttgtccaat
    21001 ggcaaaagct gccgtgtttg gttctttttc atggaattcc ttcaagactt tttctgcatc
    21061 agcgtaagat tggattccat caattgaaag atcgtattta tctagtaaag ctttgttaaa
    21121 agtaagtact tgttgagcaa agacatttcc attgactggg aaagcatata attttccatt
    21181 gaccagattt cctttaatat atgcttcatc taaatcatta taagcttttt cagcgtactt
    21241 aggcgctaat tcagtcaaat cagcaaaggc tcctttttgg gcattggaaa catagttgtt
    21301 cgcaaaggcg atatcataat tttccccgga agaaatgatg acgttcattt tcttttcgta
    21361 atcgccccag ccaatgtaat tgatattgac ttttacaccg atctcttttt cgattcgttt
    21421 gtttgctact tccatcaatt gatcgaaatt atctggttta tctccgattt gatacatctg
    21481 caaagtgtcc gaattgctag cttctgcctg gtctttagaa ccagaacctc cgcatgctgc
    21541 taatgttccc aacaacaacg ctgaaacacc tgtaaaggct agtttcttcc ataacttcat
    21601 ttttcttcct cctaatttta tgatttatat ttttgtttct ttattctttt actccgccga
    21661 ttgttagacc tttgacaaaa tgcttctgga aaaatggata gctgatcgca ataggcaacg
    21721 tcgagatgac tacgatcgcc attctggcag attctcctgg aactgaggct aatccgcctg
    21781 tcaattggct tcctgctcct gaattttgtg tcagatattg aatattactt tggattttca
    21841 tcaaaagata ctgtagtgga actagcttat catcttggat gtatagtaaa gcattgaacc
    21901 agtcattcca gtaacccaat gcggcaaata aactgattgt cgcaatacct ggaacagcta
    21961 acggtaaaac gatctgaaca aatattcgca gttcgctagc tccatcgatc cgtgccgatt
    22021 caataatcga atcagagacc gaacgtttaa agaatgtgcg catcacaata atattgaagg
    22081 gactgacagc cattggcaag atcaatgccc aaatcgtatc ttttaactga agaagatttg
    22141 tcataacaag ataattggcc accatccctg gtgagaacaa catcgtaatc aaacaaaaaa
    22201 cggtaaaaaa acgacggaat ggaaagtttg accgtgaaat cgcataagca tataaagaag
    22261 tagccgtgct attgatcagt gtacctaaaa cagtaacaag tacagtgacg aaaagcgctt
    22321 ggaaaatttt atcctgcatc tggtcgaaca agtacgtata accaaatata ctgaactttt
    22381 ctggccaaaa actgtacccg tgagtcgaca atatggactc atcggtaaaa gagatggcga
    22441 taacaaatat aaatggcaaa atacaggaaa tagcaaagag tgcaatcatt atattagaga
    22501 aaaaattagc cgtcttattg aaagagcgga cttggactct ctccactttc tttttttcat
    22561 ttttttcctc cttagaataa tgcagaatcg ctatcaaaac gtcgggcgat cgtatttgtg
    22621 atcatcaaca agacaaagcc gacaactgat tggtaaagtc ctgcagcagc cgtcatacca
    22681 aaatctcctg tggatgtcag gccattgtag atatacgtat ccaaaacttg agtcacatca
    22741 tataatgcac cggaattcct aggaatgtta taaaacaggc caaaatcagc tcggaaaatg
    22801 tttcctacgg ctaaaatcgt taaaatcgtc attagtggaa taagttgtgg tatcgtcaca
    22861 tgtttgatct gctgccattt gctagctcca tcaaccatag cagcttcata ataagtcgga
    22921 tcgattccca taacagatgc aaagtaaata atgctgttat aaccaatccc tttccatgta
    22981 ccaatgaaca ccaagataaa cggccaatat ttcggttcat tataccaatt gacagcttct
    23041 cctccatgtc cagtgatcca ctgattgaat attcctttgt caggacttaa aaaagcgtaa
    23101 acaaagtaac tgataatgac ccaagataga aaataaggta gtaaggacat cgtttgataa
    23161 actttaacga gtcgtttatt tctcaactcg ctcataatga ttgcaaatac gactgagatc
    23221 agcagattca aaaggataaa tgctacatta tataaaattg tatttttagt gatgagaaat
    23281 gcttgatccg aagaaaataa aaatttgaaa ttttcccaac ccacccaagg actttccctc
    23341 aagctagcca agaacccatc tgctgaaata tgaaaatttt tgaatgctac gacattcgcc
    23401 attacaggta tgtaaaagaa aaaaatcatc caaagcatac ccggtacagc catcaggaca
    23461 agtgctttat aacgccatag attcataaaa aaatgattga acccctttcg ttttttcatc
    23521 taaaaacccc tttcgattca ttcgcaacgt aattatagcg ctttcaataa tgagaataaa
    23581 taaacaaagt atagggaaaa tgaaacaaag tataggagag gttgtgaaat cagcgttttg
    23641 gcctgagtat tagagaaaaa aacgggaaaa tagttcctca tattttgccg ttttttgagc
    23701 taataccgag ggtcagctga ttgaacaccg tttatcagag gttgtgaatt cagcgttttg
    23761 gcctgagtat tagggagatc gtgtccttac tatctaactc tgagtcacaa ggaacaatgg
    23821 ggactgtaac aaaagtcttg tcacagtccc ttattccgaa caaacggtgt tccagaagta
    23881 gcttctacgg aaataagccg aaatttcaca aaaaagccat ccgaaagtca gacggacaag
    23941 tctgcttttc agatggcgct acaactactc tgcttattct tttagctatc cattcaatta
    24001 tttttctctc acttgttcta gctcttcttt ggtcatgggt cgatattgcc ctttattcaa
    24061 atctttaggc agccaaaatt ttcccatacg gattcttttt aaatataaga cttcttttcc
    24121 tactgctttg accattcgct taacttggtg aaactttcct tcatgcaaaa tcagacggat
    24181 aattgaagtt tctgtctctt gatctacttg atcaatttgg agttttgccg gcaatgtctg
    24241 ctcatcgcca tcaataatca gtccttctgc aaataaacgg atatcttctt ctgtcattat
    24301 accgctaacc tcagctagat attctttctc cacatgtttt ttaggtgata gtaactgatg
    24361 ggcaaacacc ccgtcattcg ttaaaatcaa gaggccctct gtgtctttat ctaatcggcc
    24421 gactggaaaa agatcttccc gataatcctg gtcttccaat aaatcgatta cggtttgatc
    24481 atgattatcc tgagtagcag aaatgactcc tgcaggttta tgcaaaatgt agtaaaaatc
    24541 tttttgatac gtaatgattt ctcctaaata agtaaccgca tcttcgaatt catttacttg
    24601 cgctttatcg cttttcacca cttgtccatt gacttcgatc gttccttttt tgattaatcc
    24661 tttgacttct ttgcggctgc caagtcccat atctgctaaa aacttatcta aacgcatgcg
    24721 tttcccccat ttcaaaagag catcaatcta gctcttctga ttcacttatt ttatttttaa
    24781 tttatgtcgt aaacgcgcta ttcctggtcc tagtagtctt tctgccaagc ggattttcaa
    24841 agctaaataa ccatacacta aagcccctat acctgctact acaagaatca acaatagaga
    24901 ctggaatttg ctatcttcgt ttaaaaaagc accaaaaatc atctttgtga tccacgaagc
    24961 caaaagcata atcaaggtaa gaataaagat cagcaatgtc ctgcgccaga caaaacttgg
    25021 tttaaagcgg gctacttcat ggattctctt taagatcaag gtacaagaca cagcaaatcc
    25081 gatcattgtc gccaataaag ggccgtatac ttcaaataaa cgaatggctg ggtattgaag
    25141 aaggagtttc actagaaacc caataaccaa ataattaatt gccgccttgt tttcaaacat
    25201 tccttgcagc atgttggaaa ctagcatata tagtcctagg aagagtccta caaaacttgc
    25261 ttggatcagt acattacttc ctagagaatc cggtgtataa aataatgtat ttagcggata
    25321 ggcaagtaac ataacaccaa atgtcgccgg aaacataaaa aatgaaaaca actgtaaatt
    25381 tccgcttgtc aattttgcca gacctactcg atctttcaac gtaacagctt ctgtaatcaa
    25441 cggtaaccca gtagacgcga tcgatgttgc caatgcaata actaccatcg tcaacttatc
    25501 tggattggca ctaaaaatcg aaaaaagatc cagtaactgt gcattggaat actcggtgga
    25561 tgcagacatg atcttcataa aagtgaactg atcgactaat ttgaagatcg ttacccctga
    25621 acccacaata ataaacggaa tcgcttcttt aacggtatct aacaacaatt ccttcgttgc
    25681 aatatttatt ttgttttcgc tgtagtgaat aaacgccgat gtatacgctt gatgtttctt
    25741 caaaaaatac aaaaggacaa gaatacttgc tagcataccg ataaatgccg cgaaagttga
    25801 ttgcgtgacc gccgtcacat agtcaccatc catgactttc atgatgatga atgtagccaa
    25861 tagcatataa aaaatacgtg ccacctgttc tacaatctgc gacagcgcat aaggcatcat
    25921 ttcttgattg ccttgcaaat accctcgtat cacactcata caaggaaaaa ccaatacagc
    25981 gatactcaaa gaacgcatcg ttggaatcag ttcttctccg ccaccagaag catgagctag
    26041 ccaaggagag gcgatgtaca tgaacaaagc aaacaacagg cctaacccgc ccatcatctg
    26101 taatgcacgg ataaataatc gtcgtgaagt gccatattca ttcaatgaat tatagcgtgc
    26161 agtttgtttt gcaatcgcag caggtattcc tgctgtagag atcagtagaa acaatgcata
    26221 tatattgtat cccatattga ataatccatt ggcagcctta gcattttcgc ccatccaagc
    26281 ataccaggga atgatataga cagcgcctag caatcttgaa atgatattac tggcggtcat
    26341 ccaagcagag cctcgtgcca ttttttcttg cggtgttaat tgatctaatg ctggttttct
    26401 ttgattatcc atcggtcaga ttccaacttt ctacaaaaca ttactatcat tttaattggt
    26461 aaaacagaaa ctggcaatcg attttatata acttttataa gaacaattgt ttttttgaat
    26521 tcggtttcgt ttttttggta aagtaaagaa atatacagcc ttttatggct aacatttata
    26581 ggagatatca atgacacaag aaataaaaaa actacccgtt atatttttag gtcttttctt
    26641 tatcacagtt ggattgaatt ggttcttgct ccctcacgat atagcttccg ccggagtggg
    26701 cgccatcgga catttagttg aaacggcctt ttctatccaa cgctcagtta ctgtttggag
    26761 catcaatctt atgatgctgc ttctagccgc tgtcctatta ggaaaatcag tattttttaa
    26821 gactgtagcg ggaagtctgc tttttcctgt tgttttagag attgttccaa aagtggaaat
    26881 gatctcctca catttctttt cactgatttc aggaagtctt ttgttcagtc ttggggttta
    26941 cacattgtac acagtcgggg cctcaaatgg cggtataacg ataccgccga ttattttcca
    27001 caaatttttt cggcttccaa tgccgcaagg acttttactt accaatagtc ttatcgtctt
    27061 tttgaattat tatgtttttg gattgttgga gacactattc gttctcctat ctattacagt
    27121 cagttcattg tttatgaaaa tactcatccg cttgtcccct gtgtcaagaa aatttgaata
    27181 aagctggatt ttgtcatagc ggaaacaaac gatatattaa aaagagtgtg ctataataaa
    27241 ccaagaaatt gaataaagcg agggatcaat caatgagctt ctctttagaa gacatccgtc
    27301 atttactatt aaaagaaaat ctattaaaag aatttgtttc aacaagaggc tggcatcttg
    27361 atgtaccaga aaaagctgaa tttcttcagt taagttatga ttcaagaaaa gctgatgctt
    27421 ctactttgtt tttctgtaaa ggaatgaatt tcaaagaaga atatttggat tcagcaatcg
    27481 aacagggtat tcaatattat gtatcagaac aaccatacga aaattcagca gtaggaatca
    27541 tcgtaacgga tatccgtaaa gcaatggcgc ttcttgctat ggctttttac gactatccgc
    27601 aaaataaatt gaaagtgatc ggattcactg gaacaaaagg taaaacaact gctgcgtact
    27661 ttacaaaagc aatcttggat cacacaacaa ataaaaaaac agcattgctt tctactatga
    27721 atacaacgtt agatggcaaa acatatttca aatcccattt gacaacgcct gagtcattag
    27781 atttatatcg tatgatgtca gaagcagttg aaaatggtat gactcatcta gtgatggaag
    27841 tttcttcaca agcttacaaa acacaacgcg tatacggctt gactttagat gtcggaatct
    27901 tcttgaatat ctcgcctgac catatcagtc cgatcgaaca tccgacattt gatgactatt
    27961 tctattgcaa gcgacaattg atcttgaatt caaaaacagt tgttttaaat catgaaagtg
    28021 actattttga tttacttagg gaaacagccg atctattcca gattccaacg atcacttatg
    28081 gtcgttcgga agaaagcaat tatcaagtga tccgttcaga taaaggtact cacggtttta
    28141 ctttatcttc tcatgaagat cgcttagcta tctgcgatac tgcttatgat attttgctgg
    28201 ctggaggatt caaccaagaa aatgcagcca gtgcgatcat tgccgcagca ctaacaggtg
    28261 cctccagtca agatgcacaa gacggattaa aagaagctcg tgtaccaggc cgaatggatc
    28321 aattgatcca atccaatgga gcgcatgtct atgtggacta tgcacataat tatctaagct
    28381 tgaaaacatt gctgaaattt gccaaaaatg aacatccaga tggccgtgta attgttgtat
    28441 taggcagccc aggaaacaaa gcaatctccc gtcgacatga ttttggaaaa gtgctatcag
    28501 aaacagctga catcgccttt ttgacggcag atgacccagc atttgaagat ccgaaaaaaa
    28561 tcgcggaaga aatcaatgaa gcaattacta atccagattt gatcgttcat tatgaaatgg
    28621 atcgtcctga agcgattcga cgcgctttag cagaaagcgc tccgcaagat tcagtcgtta
    28681 ttgcgggaaa aggcgtggat ccctatcaaa aaatcaatgg tgtagatgaa ccttatgaag
    28741 gggattatgc aatcgtgaag cggttgattg aagaataatg gtatttatat actagtggtt
    28801 tcccatttct aagaaaaaaa gggactgtga ttttttgtca cagttccttt tttcttagaa
    28861 atcattttaa cttttcacta gctaattctg ctgccttttc tctttcttca tttaataatt
    28921 tattatcata gaatttgata aatgggaacc agataatgaa tgcgatgatt gcacaaatga
    28981 ctgctaacac agcagctttc caatcaccac cactaccaac aaatgctccg atcccgactg
    29041 gtgaaggcca cggcatctgt gcaagcatcg gtttgacgat ttccagccga atggcaaaat
    29101 aagcgagtgt agcagaagcc atcggagcta agaaaaacgg aaccgctaaa tagggattgt
    29161 agacgattgg catcccgaaa atgattggtt cattgatatt gaataatcct ggtacaagtg
    29221 aggcttttcc taacactttc agctgatcag atttcgcgag gaatgcaata aagatgatca
    29281 atcctaaagt cgcacctgat ccaccaacgg ttacataaga attgttgaat tctcctgcca
    29341 acggataatt tgcgccttgt gcgttagctg ccatattagc taatacgatc ggggtcaaga
    29401 aagaagtgat gatattcgct ccatggatcc ctacgatcca caatgcatgg atcaagaaat
    29461 aaatcaccat gacaccaagc catgtattcg tcaactgcgt aacaaaccca aatgggatag
    29521 caatcatttt gaagatatcc gttcctagtg caactaataa tccattgatg atcatcacca
    29581 caaacgcaat gacaaatgtt gggatcagag cagtgaaaga acgagaaact cctggcggaa
    29641 caacatctgg catttttatg acccaatttc tcttgacaca catacaatac aattcgaccg
    29701 caataatcga cataataata gctgtaaaga ttcctgaagt tcctaaacgt tcgacaaaag
    29761 agcccatcct aataccattt actacagttt ctgtctcatt gatagaagca atcaagctga
    29821 ttttaccatc agtcatgatc aattcaggga tacacataaa gaatgcaaaa acagatagta
    29881 atgcccccgt gagcggatta acatttaatt tttgttcatc tcgttcaatt gacgttaatt
    29941 cataagcaaa cacaatggca aagtaaatag ataaaatccc catcgtcatt gtattgacga
    30001 tcatgtacaa gtcactaact ttgaaaaaag ttgcattaaa aaatccttct aaaaatggga
    30061 acggtaatgg caaaatattt aaaaccaaaa acatcgagcc tacaatcgtg aaaggaatag
    30121 aagccatccc cgctgccatg acagcccgaa cgattttaag ttgcgcaatt ttccccatag
    30181 gtcccatcag gtatttctgc aaaaaatcaa acattagtta ttctcctttc ctaaatataa
    30241 cgctttttca tattgtttaa tacggatata ctgttggtct tggttgagtg aaattttctt
    30301 taaacgatac tgtataaatg cactggcaaa aataccgctt acaacaagaa tgaaaacaaa
    30361 taactgtcct ccattgttat ttgccataaa aggatacaag aatgtaaatc ctgaggtaaa
    30421 caacactgga atcactaata aattaaagac actctggaac aaaaaatacc actttgtcca
    30481 tggaacgttc gtttggtgtt ttcgataaag ctttacttgt tctcctactg caggcaaaat
    30541 agctaaaatc agaatcatcg gtaaactact tcctaaactt ttagctgata gaagcagaat
    30601 catccattgc atgttcatga aaaagaaaaa ggcagtcagg taacgtacga ttagataacg
    30661 attgaaatac atgtttttca aactaagttg ctttttttct aattcgattt tcctttttac
    30721 tgtatcctgc acaatgatcc ttcctttcta tttatttttt tgatacaaat aattcatttc
    30781 ctgtgcaact tccaataatg tcatcgtcgt catcagatga tcttgtgcat ggaccatgat
    30841 aatttccatt tctatttttg tcccgctggc atattctttc agaagttgag tttgtgactg
    30901 atgcgcatga agaatctctt catttgcttg tcgcaacttc tcttctactt cttcaaacat
    30961 ctctttacgc atcgagcgaa atgcttcatg tatcagtgtc cgtgcatttc cgctatggaa
    31021 aatgatatca aaagaagtca cttgtagtgt ttcactgttt attttttcag ccatctgttt
    31081 tcctccttat tcgatcagtt tgataaattt actgctgaaa ttctcaaaaa taggttcagc
    31141 taagatttcc tgctgtactt ttagctggtc aactaaccta acgattgctt tagtcacgac
    31201 cgtaattcct tcattttcga cataagaagg cgaaactaaa aaaacaaaat gaatttcttg
    31261 atattgatcc cactgcatcc cagaaggaat caatgcaacg gctacctttg tttcgattcc
    31321 aactggaaca gcgggatgtg ggactgccac tgtatcactg aatatgattt gtcccatatt
    31381 ttcacgttgc tggatctgct ttcgcatttc tcttatatat ccttcttctt catttatgct
    31441 cagcttttgc agcaaatcat gaagaatttc ttcctttttc ggttcgtacg tataagtctt
    31501 gaaaaatttt tcagacaact gttcggtgta aatctgcttt ttttgctgta gagaaaatgc
    31561 tggtatcggt ctatctgagg aatgataggg tatagattct ccaactacct ttcgtatttt
    31621 acgaacatca tcttcattca aaaaaatact gacatgtaaa actggaattt tgaaaaacat
    31681 tgtcgaaaga tcaatagaag aaataatcag gtcgattcct tttaaagttg tttcattgat
    31741 ctcataatag ccttttacac ttaccactgt aatattttta ccgaattcac tgactacgcg
    31801 attctttaat agttgggcac tgccgtaacc cgttgcacag ataatcaatg catgtagttt
    31861 atgcgcagct ctttcttttt ccaatgccgc catgagatgc aaagccaaat aggcccattc
    31921 atcttcattg atggtatagt ttttcaaagc cggcatgttg ctgaaatgcc tttttactgc
    31981 gtcaaagact ttgggatttt ctttctttat ctcctcagtc aatgggttct caatgcgaat
    32041 acctcggctc aatcgaatca tcatcggtga caaatgatcg agtagtccat tgaaaagctg
    32101 gagatcctct actaatgaaa aatgaaattc ctgagacaat cttttcagaa ctttcgctaa
    32161 ttcctttgat agttcctgat cattttgatt ttctttgtga ttcgacttag ccatgagatg
    32221 taatgtcaaa taagcaattt cttcttcggg aaacgataca cctgcgatta cctcaatacg
    32281 ttgaacgatc cgctgcgcca cttgatactc tactcgctta gaaacttctt gttcgatgcc
    32341 taattccttg atttcaaacc cttctcggag ccgtttgatc cccaaagcca agtgtaaagt
    32401 cagattctgt atgataaaat ccgaaacttt taacttagct tctcttgttt catcgagaat
    32461 gataatgact agctcttcaa aactgatgtc tgggaaaaaa cggctttgcc ctaaatattc
    32521 tttcaatgaa tttccataat ggtttccaaa aaaagtatcc atgataaaat gccgtcgatt
    32581 tctttcttcc ccttcgatcc atgtcccttg accatgctta gaaacgattt ttagtgaata
    32641 tggaatcaat cgctccttga tttcttgcat gtcctttgtc aaactggaac gactgatata
    32701 aagctcttct gccaaatcat ccataaaaag aatggcatct tccaaaagca attggttcaa
    32761 tatatacttt tgtcgatctt cggactcata aaatccttgc ggttctctag catatccagg
    32821 tactatttct tgccgactca aaaacaagtc aaatgtcagt ctttgtaaaa tatgcagttg
    32881 gtagccataa cctggtttag caatgatctt acctccgttt ttttctacta actctttgat
    32941 ccgctgcaag taattacgga ttgtccgatc agataataac aattcagaag ctaaacgttg
    33001 actggtgaca tactccttct tgtgatcaat caaatatctc aacagcaatt tttccttctg
    33061 tttcattatc atccactcct attccatcat atgttctctt cgatcacctc tgctaacttc
    33121 tggatgcctg tagggattgg gacatacgct tggaaaggga tactaacaac aggctttcca
    33181 acagattctc ctaatttttt gaatttatcc aaaaacatcg tcgtttgcgg gctgatcaga
    33241 aataaatcaa aatcactatt cttaatcata ttatcgcctt ctgtagctga aactgcatct
    33301 acgagtacct cttctctttt ttgcttaaag aaatccgttg tctttgaggc catcatcgag
    33361 gatgacattc ctgccgcaca aataatcaat gcttttttca taaatcaatc actcctttta
    33421 tttttataaa ccaagcatat cttgtaagcg ttttctattc aaaatatata tttccgtagc
    33481 attgcggaaa aaatgtgtat catatcttga ataataattt ctggcaccgt gatatactag
    33541 tgatagaatt ttttcaggag gggatttagt gagtatcaca gtgaatattt tgtatacaag
    33601 tacaaatgga caagccagaa aatttgcaga agaaatggtc gaaagaaaca ttgtcaaagc
    33661 gattcgtgca gaagaaggaa acgagaaata tgactattac tttccactag aagatccaga
    33721 atctctttta ctgatcgacc gttggaaaga tgaagaagca atcgaaaagc atcacaaatc
    33781 cgaaatgatg gctcaaataa acgaactccg taaaaagtat aaattaaaaa tgaaagtcga
    33841 acgtttcact gatttaccgt aaaaagatac acatggaatg atagcaattt atcatccatg
    33901 tgtatcttca ctttttcctt attttttatt cgatctcatc taatgattca aaccgtgttt
    33961 ttctcttatt tttcggataa gtatttcagt caccccaata tacatgattc ctaaacatac
    34021 cccaaggata ttctcaaaaa tgcggatcgt tactgctcct ggaaccccga ataaactggc
    34081 agtgattgcc aaagcaccaa aagaattaaa aactgtttta tatctatagg tagaacaaac
    34141 acctaaagct agaccaccta atataccgac ccagttaaaa ggaataaatt ggctgatacc
    34201 aataaataaa acacatccaa tcaatgagcc aatgattcgg tctactgctc gttctttgaa
    34261 accaatcgac atcaatccat agctagatac aatagaagaa aatgcaaacc ctgcccacat
    34321 caatcgctga aaagggacat actctcctgc aacgatcaaa agactaatgc caaatgcata
    34381 gatgagcatc caaatttttt cctgcgaata catgcctttc aaaaaaatct ctttaaacaa
    34441 gcttttgtct ttgttctttt ctcgatgttt tctgtaaagg agaactgaaa accaacaaaa
    34501 aaacaaaaat aataagctac ttgtttgtgt aaagaaattc ttgttcaacg aatctttagg
    34561 taacgaataa acaataaaga gatatgaaaa gccgtacaaa gaagcattcc ccattttggg
    34621 atcactagcg gttgccatta atatgctgct tagtaaaata aaatgaatca acatgcccat
    34681 aactgaccat ttgatcagct ggacatacgg tgctactaat agtgagaaca tgacgattgc
    34741 taaacctatg atcgaatggg aaatcttata accaaagtca acaaaacgaa tactcatcaa
    34801 catacagaat aaaaccacag cgtatggttt atgcttcgat tcaaataatg ttgtaattat
    34861 aatgataaaa caaatggcaa acaatacgat gaataatgat cgtaaaaaca atgaacaaat
    34921 atacatgcac ttttcttttt tagaagctgc ttctcgtatt ttttgtttta aaataaacgg
    34981 atctagttgt agagactgat aaaaatccac ctgttgtcgt cctttctttt tacttttatt
    35041 ttttacagtc atctatgaca ggttgtaaat actatggaac atcatatggc gttgtcattt
    35101 tcttgtcaac tatttttcct attcatgttt ttataaaaaa acatatcgtt ttcttgtatg
    35161 attaaatcac cacaaaataa acaactgaga gaacgatatg ctttatgaat ggttctatca
    35221 aaaaaattct gagatcaata gacaaaaatt tgatgattac agaggtctct tacgaaacct
    35281 tacagaagaa aaaaacattg atcgttgctg cagttctctt ccctgctcca tgtgtctgta
    35341 gaaattgtgg ttctgctgtg gtagatggaa ataggaaatt aatggtggtg aaaaacaaga
    35401 agaaggaaac cattgtccac tttgaataat ataatcatat gccaatggtt atacgcctaa
    35461 aaaagcagcg ctatacctgt aaaaactgtc gaagccattg gaacgctcaa agttatttta
    35521 tccgtccaag gcactcgatt tcaaatcatg tcagacataa aatcacttct ttactgactg
    35581 aaaaagtgtc gttatttttt atttcgaaaa gttgttaggt atctttgaca accgttattc
    35641 gcacattgaa agagttgaga agctatttac caaagtcttc caaaagaatt ctgtcttgag
    35701 tgttgagggt tgtgaatttc gttctcacgt ttccgttgag gataaactga gttttatttc
    35761 cgcggatggc gagacagaaa aattaatcga gaaaatttac ctacacaaaa aattcctcga
    35821 ttaacaaact atttccttag ctgcactaat cctaaagaaa tagaattctt agttacagac
    35881 atgaacgcca cctactttca gctcaccaaa catgttctac caaatgcgaa attggtgatt
    35941 gatcgattac ttggtttttc cacgcctttc aaggaggcgt aacctttatt cacgagttgg
    36001 ttgaagcttt ccgagacaaa agacccgact tgtttttctc cttattgaca aagagccatt
    36061 aaaactagag aaaaaattac aactgatcgc tgattatttg tagaaagaaa atcaaagtat
    36121 tacccaataa acttgagtca ttaggcaaaa aacactgcga aactcaagaa tgattttcgc
    36181 agtgtttttg tatatttttt attaactagt attactacaa aggtttattt ttcacttctc
    36241 aacgcagcac aaaattgtct ttacttattt actggttggg ttcaaccttc tctactccga
    36301 tactaatatt tgtttgtata ccccttctaa aatcggtagt ttccattcaa ttgatgggta
    36361 ctagttgatt ttaaaaacta tgcttattca attttttatt ttagctcata tatttcgttt
    36421 ctttctaaat tttatactga tgaaaacaag tagcacaccc aatattgaca atataaactg
    36481 gctaatgatg gtttctccag tttttggaag atgcttattt ccactgcttg ttggattgtt
    36541 tcctcttata tctgagctat cagcaacttt tctattctct tttgttaaaa agatcgccac
    36601 ttcttgttct ttttcgatct tgaaagttac tggtgtcgca tccaattcat accccgttgg
    36661 cgcttgagtt tctaccagct gatatgctcc tggtgctaag ccatctatcg ccagttttcc
    36721 atctgctcct gtggttagac ctgactgcag aacggttcca ttggcatctt gtaactcaaa
    36781 aacagctcct tgcaggactt ctccgctttg gtcatcgatc tttgtcaaca cgacaccgcc
    36841 tggtgtaagt cgattttctt tcgtcaattc taccaccgct gtctgacttc tttcgatctc
    36901 aaactcgatt ggtgtcgcat ccaattcata ccccgttggc gcttgggttt ctaccagctg
    36961 atacactcct ggtgctaagc cgtcaatcgc cagttttcca tctgctcctg tggttagacc
    37021 tgactgcaga acggttccat tggcatcttg taactcaaaa acagctcctt gcaggacttc
    37081 cccgctttgg tcatcgatct ttgtcaacac gacaccgcct ggtgtaagtc ggttttcttt
    37141 cgtcaattct accaccgctg tctgacttct ttcgatctca aactcgatcg gtgtcgcatc
    37201 taattcatac ccc
//
LOCUS       DARBDV010000014        35235 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000014 DARBDV010000000
VERSION     DARBDV010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.014"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(181..666)
                     /locus_tag="SCM56_000739"
     CDS             complement(181..666)
                     /locus_tag="SCM56_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HEG1109561.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    756..1101
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1146..1787
                     /locus_tag="SCM56_000740"
     CDS             1146..1787
                     /locus_tag="SCM56_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEG1109562.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1806..2441
                     /locus_tag="SCM56_000741"
     CDS             1806..2441
                     /locus_tag="SCM56_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEG1109563.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2459..3274
                     /locus_tag="SCM56_000742"
     CDS             2459..3274
                     /locus_tag="SCM56_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG1109564.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3593..5029
                     /locus_tag="SCM56_000743"
     CDS             3593..5029
                     /locus_tag="SCM56_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG1109565.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5218..7665
                     /locus_tag="SCM56_000744"
     CDS             5218..7665
                     /locus_tag="SCM56_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEG1109566.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7895..8962
                     /gene="rlmN"
                     /locus_tag="SCM56_000745"
     CDS             7895..8962
                     /gene="rlmN"
                     /locus_tag="SCM56_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEG1109567.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(9155..10258)
                     /locus_tag="SCM56_000746"
     CDS             complement(9155..10258)
                     /locus_tag="SCM56_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEG1109568.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10811..11359
                     /gene="aac(6')"
                     /locus_tag="SCM56_000747"
     CDS             10811..11359
                     /gene="aac(6')"
                     /locus_tag="SCM56_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HEG1109569.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            11905..13566
                     /locus_tag="SCM56_000748"
     CDS             11905..13566
                     /locus_tag="SCM56_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG1109570.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(13636..13983)
                     /locus_tag="SCM56_000749"
     CDS             complement(13636..13983)
                     /locus_tag="SCM56_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HEG1109571.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            14067..15008
                     /locus_tag="SCM56_000750"
     CDS             14067..15008
                     /locus_tag="SCM56_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1109572.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            15009..16052
                     /locus_tag="SCM56_000751"
     CDS             15009..16052
                     /locus_tag="SCM56_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1109573.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            16058..17128
                     /locus_tag="SCM56_000752"
     CDS             16058..17128
                     /locus_tag="SCM56_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1109574.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            17118..18071
                     /locus_tag="SCM56_000753"
     CDS             17118..18071
                     /locus_tag="SCM56_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1109575.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            18083..19093
                     /locus_tag="SCM56_000754"
     CDS             18083..19093
                     /locus_tag="SCM56_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1109576.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(19378..20199)
                     /locus_tag="SCM56_000755"
     CDS             complement(19378..20199)
                     /locus_tag="SCM56_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEG1109577.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(20296..20574)
                     /locus_tag="SCM56_000756"
     CDS             complement(20296..20574)
                     /locus_tag="SCM56_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109578.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            20842..22851
                     /gene="metG"
                     /locus_tag="SCM56_000757"
     CDS             20842..22851
                     /gene="metG"
                     /locus_tag="SCM56_000757"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEG1109579.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(23330..23917)
                     /locus_tag="SCM56_000758"
     CDS             complement(23330..23917)
                     /locus_tag="SCM56_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109580.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            24092..25414
                     /locus_tag="SCM56_000759"
     CDS             24092..25414
                     /locus_tag="SCM56_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1109581.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            25569..26345
                     /locus_tag="SCM56_000760"
     CDS             25569..26345
                     /locus_tag="SCM56_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEG1109582.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            26338..26907
                     /gene="rnmV"
                     /locus_tag="SCM56_000761"
     CDS             26338..26907
                     /gene="rnmV"
                     /locus_tag="SCM56_000761"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEG1109583.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            26900..27784
                     /gene="rsmA"
                     /locus_tag="SCM56_000762"
     CDS             26900..27784
                     /gene="rsmA"
                     /locus_tag="SCM56_000762"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEG1109584.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            28235..28864
                     /locus_tag="SCM56_000763"
     CDS             28235..28864
                     /locus_tag="SCM56_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109585.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(28988..30097)
                     /gene="ugpC"
                     /locus_tag="SCM56_000764"
     CDS             complement(28988..30097)
                     /gene="ugpC"
                     /locus_tag="SCM56_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEG1109586.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            30306..30728
                     /gene="mgsA"
                     /locus_tag="SCM56_000765"
     CDS             30306..30728
                     /gene="mgsA"
                     /locus_tag="SCM56_000765"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HEG1109587.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            31051..31587
                     /locus_tag="SCM56_000766"
     CDS             31051..31587
                     /locus_tag="SCM56_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEG1109588.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(31627..33390)
                     /locus_tag="SCM56_000767"
     CDS             complement(31627..33390)
                     /locus_tag="SCM56_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1109589.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(33393..35108)
                     /locus_tag="SCM56_000768"
     CDS             complement(33393..35108)
                     /locus_tag="SCM56_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1109590.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT        11462 a         6280 c         7302 g        10191 t
ORIGIN      
        1 ttcttttttc gtagaatcaa taaatatgga tttggctaac tatggaagaa tactcgtatt
       61 agttttcagc gttcatacta gttgttacat tgtttgattg gccaaaagaa tttctgatat
      121 attttgtgct aaataaagaa aaagacgctt gaattgggaa ataagaaaac tactctatca
      181 ttacaaattt tcataaactg gcagatgttc taagtaaaca agtatctttt caaagtcgga
      241 aaaatccata atcaacgtag tggtattgat atttggatga aagcctacgg tatcttgacg
      301 atcaatagca gcatcgatca cgacttggat ttttttctct ttgtcgtgca tcaatgctaa
      361 tggagtcact gtacctgctg gaacgcctag gaggtcagga agctgttctt cagaaacaaa
      421 agataagcgc ttttctttta attgttcagc tagttgtttt aaatcggcgt gcttttcatc
      481 aggcaagatg acaagataga tttgttttcc ttttcttgat ttcaataata gattttttac
      541 ttgcggacca ggtagggaaa aagggacatt tttgactgac gtaatcgcag gatgatcgac
      601 tttttgaaaa gagataccca gtttttttag tagttcataa gcttcttgtt cggttgcacg
      661 actcatcgcg catcaccctc ctttttttat tagtgtatca taaaaaagag ttgacaaatg
      721 aaaaataatc atgtaaatta cacgtaaagt taaaacaaat gacatcgaaa agaagagtag
      781 ccgttaggtg tttttagaga gtctctggtt ggtggaaaga ggcagcagtt aaccgtgaaa
      841 cacatcttgg agtcgatttt ctgaagcgta tagtaggaaa atccggggag cccgttacag
      901 ctgcggtcgt tgctagatga ccagataagg ctggcttttc cgtgaggagt cggtaaaaaa
      961 aggtggaacc acgtgattaa cgtcctttga tcttcggatc aaaggacttt ttgttttcat
     1021 ctgaacggca acgactgtgt gaggtttttt ctgcaaggaa aaaataaaca gaggtggaac
     1081 cacgtataat acgtcctctt ggcatttgcc aagaggattt ttttatttta aaggaggaaa
     1141 agaagatgga gtatttgatt gaagttatgc cgcaattatt agtaggagca ctcacaacat
     1201 tgaaattatt tttatttacc ttgattggct cattaccatt agggattgtt ttagcattag
     1261 gattgtcttt gcattttaag ccgctacgct acttgcttca aacatatgta tgggttatgc
     1321 ggggaacacc gctgttgttg caactgattt ttatttttta tgggttgccg acgattggga
     1381 tcgtttttga tcgtttcgaa gcagcactca ttgcctttat cttaaactat gcagcttatt
     1441 ttgcagagat ctttcgtgga gggatacaat cgattcctca aggtcaatat gaagcagcgc
     1501 aagttttgca attaagtccc tttcaaacgg tgacgaaaat cgtcatccca caagtgacaa
     1561 agattgtttt accttctgta gggaatgaag ttatcaactt ggtcaaagat tcttccttga
     1621 tctatatatt aggtttaggt gacgtaatgc gtgctgggaa aatcgcgatg gaacgcgacg
     1681 cgactttact gcctttgcta ggcgtggcaa tcatctatct tacattgact ggtattctaa
     1741 caatcataat gaaacaagtt gaaggaaaat taaattatta ccgctaactg ataggaggaa
     1801 gaaatatgat tgaattgaag aaagtgaaca aagcatttgg tgaaaaaaaa ataatcaaag
     1861 atttgaatct aatcattcca gatgggaaaa cattagctat cgtaggtcct tccggtggcg
     1921 gtaaaaccac gcttttacgt atcttggctg gcttagaaag ttctgacagc ggagaatttt
     1981 atttagacgg tgtaccgttc aacccattcg agcttaaaat gaaagaacaa gttgtaggag
     2041 tcgtattcca agacttccag ctatttccac atctgaatat ttttgaaaac attaccttag
     2101 caccaaaaat ggtattgaaa cagacaaaag aagaatacac caaaaaagca gaagaattag
     2161 cggcttcatt agggattagt gatttattga atatctatcc ttatcagctt tctggcggac
     2221 aaaaacagcg ggtagcgatt gccagagcat tggctatgaa tccacgggta ttggcttatg
     2281 atgaaccaac aagtgcttta gatcctgaat tacgccagca agtcgagcgt ttgatcctat
     2341 cattgaaaga agatggggta acccaaatta ttgtcaccca tgatttagta tttgcagaaa
     2401 atgtcggtga tcaattacta aaagtttccc caattcatta agaaaggaga agatagagat
     2461 gaaaaaaacc agttttctat tcgctttgat cgcaggtctt cttctttttg caggctgttc
     2521 gaacaaaaaa acttcagcag ataattgggc gactatcaac gaagagaaaa aaatcgttat
     2581 aggtttagac gatacatttg ttcctatggg atttagagat aaagacggga agctcacagg
     2641 atttgatatt gatttagcca aagcagtctt tgaacaatat gggattacgg tagactttca
     2701 gcccattgac tggtcaatga aggaatttga actgaacaat ggaacgatcg atttgatatg
     2761 gaacggctat tccaaaacgc cggcaagaga aaagaaagtg caatttacaa aaccttatat
     2821 ggaaaacgat caagtgctga tcacacctaa agaatcagga atcacaaact ttggacaaat
     2881 gaataataag agattaggtg cgcaaaatgg gtcatctggt tatgatgtat ttacgaaaca
     2941 accagaagta ttaaaagaga tagtcaaaaa ccaagatgct gttttgtatg atagttttaa
     3001 tgaagcattg atcgatctga aaagcagacg ggtcgatggt ctgctgatgg ataaagtata
     3061 cgcagactat tatttgaaac aaagaaacga actagaagac ttcaacataa caaaaggttc
     3121 ttatgagagt gaggattttg cagttggtgc acgcaagtca gatagtacat tagtgaaaaa
     3181 aatcaatgaa gccctagatc aactagagaa aacaggtgaa ttccaaaaaa tatccgataa
     3241 atggtttggc gaagatgtta cgccgagaaa ttaacaaata ggggactgtg acaaaagacg
     3301 tgtcacagtc cctttttccg aataaacagt gttctcctgc caactccttg tgactcggag
     3361 ctgaatggca ggctcacaac ctcttggtaa acggtgttca aaagccagtt tttcggtatt
     3421 aagccaaatt tatgcaaaaa tgtagaaagc cattttcaca aaatttttct tactactcga
     3481 aactaagcga ctttttcaca acctccttta cataatttta ttttgttgtg gaaggggttt
     3541 cgtgttattc taaaagagga acgattgaag gttataggaa aggaagagat gaatggaaca
     3601 caaagaactt acctcatttc caaaagattt tctgtggggc tctgcttctg cagcgtatca
     3661 agtagaagga gcttggcaag aagatgggaa aggagaatca gtctgggatc gatttgtacg
     3721 gatacctgga aagacattta aaggaacgaa cggtgatttg gcagtagacc actatcatcg
     3781 ttataaagaa gatattgctt taatgaaaga acaaggtctg aaagcttacc gcttctctgt
     3841 agcttggaca cggatttttc ctaatggacg aggagaagtc aatcaagctg ggctcgcttt
     3901 ttatgagaga ttgatcgacg aattgattga aaacgatatt gaacctatgt tgactttata
     3961 tcattgggat cttccacaag cattgcaaga tgaatataat ggttgggaat cacgtcagat
     4021 cattgaagat ttcactcatt atgcagaaac attatttgag gcattccgcg ggaaagtcca
     4081 ttactggatt agtttgaatg aacaaaatat cttcacttca ttaggttatt tgttagcagc
     4141 acatccgcca ggtgtgacag acccgaaacg aatgtatgaa gttaaccata ttgctaattt
     4201 agcaaatgca tctgtcatca ataaatttca cgagttagaa atgcctggaa aaatcggacc
     4261 aagctttgcg tatacgccaa attatccaat tgacagtaat cctgaaaatg ttctggctgc
     4321 tgaaaatgca gaagacctga tggccaatta ttggatggat gtctatatgt ggggaaaata
     4381 tccgattgct gccatgaggt tcttagaaga aaaaggatgg gcaccaacga ttgaagctgg
     4441 cgatgcagaa ttgctagaat ctgctaaacc agattttctt gggatcaatt actatcaaac
     4501 ggcgacgaat gcattcaatc cattaaacgg agttggtgct ggaaaaatga atacgacagg
     4561 aaaaaaaggt tcatcagaag aaactggagt tcctggaatg tataaaaaag ttgagaatcc
     4621 atttgtcgaa cgaacaaatt gggactggga gattgaccca gaaggattaa ggatcggact
     4681 gcgtcgtatc acaagccgtt accgcatgcc ggtactgatt acggaaaatg gcttaggaga
     4741 gtatgacaag ttgacagaag ataaacaaat ccatgacgac tatcgaatca attacttaca
     4801 aagccacgtc aaagccatca aagaagcaat ttcggatgga gcagaagtcc taggctattg
     4861 cacctggtct tatacagatt tgcttagctg gctgaacggt tatcaaaagc gatatggatt
     4921 tgtatatgtt gatcaagacg aaacacaaga aggatctttg gaacgatata agaaagacag
     4981 ttattactgg taccaaaaac tgattgaaga aaatggagag aacctttagt tcacatactt
     5041 aaaacaagaa ggtacggctg gatatggccg tgccttttct tgataaaatc atttagaaat
     5101 taagaaaaac tgtaaaataa gctgaaaaat gaaagaaagc gtcagttctt tcaaacaaaa
     5161 ctttagttcc cttacttttt tttgtataat gaaacttgat gaaatgaggg ataataaatg
     5221 gcacaaaaga aacggcctac taaaaaaagg aaaaagacga aaaaaacgaa aaagcaaaaa
     5281 cagcagcagg aacaaatgct gacgattggt gttgggtttg ccttgatttt atttgctgtc
     5341 ttcggctttt taaaattagg ttttttagga atattgattg caaacggctt tcggatcatt
     5401 gcaggaaata cttaccaaat cctctgtctt ttattagccg tattaggatt ttggattgtc
     5461 attaagaata cagagttttc aattggtaaa aaccgacgct ggtttggcgg catactcttt
     5521 tatttcggta tcttattatt gttacatgcc catttatttg gcaaactcca tactggggaa
     5581 ccaaatatca tggggacgac atgggatttg ttagccagtg atataaaaca aagtcaagta
     5641 gacaataacg ttggtggtgg gatgattgga gcaatccttt atcattttac ttacttcttg
     5701 attgcacagc ctggaagtta tttagcagca gtgctattac tggcaggagg agcattcctg
     5761 ttcagtaatc tggaaggcta tcagctgtta aatggtatcc aatcaattgg agaacgagta
     5821 caggaattat tggaaggtga tcctgccaag caagctcgaa agcaagcggc aaaggaagag
     5881 agaatgaaac agcgcgcaga agcgaaagaa gcaaggcgct tagctgcaaa agaagctgca
     5941 gaaaaagaag ctgcagaata tgagaagaaa aaagcggtga gccagcaaag ggaaaatact
     6001 gctgatgaat ggcaggaacc agaaaataca gagccggagc agctaagttt tgttccaatc
     6061 gacagttttc aagaaaatat ccatcctgcg aatctagaga aaccagtacc agatacacca
     6121 aaacagacca atacagcaga aggtttttca gatgagttgc cagaggatga cggtacctca
     6181 ttagaatttg agattgaagc agaacaggaa aatcaagact atgaattacc gacagttgat
     6241 ttgcttgatt ctataccaac tgtggatcaa agtgatgagt ataaaaaaat tgaaaaaaat
     6301 atcggcgtat tagaacaaac tttccaaagt ttcggtgtcg atgccaaggt cgtcaaagcc
     6361 agcttaggac catccgtcac aaaattcgaa gtccagccgg ctgtaggggt aaaagtaagt
     6421 aaaattgtga acttaacaga tgatatcgcg cttgctttag cggcgaaaga tgtccggatg
     6481 gaagcaccga ttccaggtaa atcactgatt ggtattgaag taccaaatgg aaagatcagt
     6541 atggtttcct ttagagaaat catcgaagca caaccaaacc acccagataa attactagaa
     6601 gtacctttag gacgtgatgt ttctggacgg gtacaaacgg ctgatctttc gaagatgcct
     6661 cacttactcg tggctggttc gactggtagt gggaagtcag tagcaattaa tgggattatc
     6721 acaagtatat taatgcgtgc taagccccat gaagtaaaac tgatgatgat cgatccgaaa
     6781 atggttgaat tgaatatgta caacggcata cctcatctgt tgacgccggt cgtgacgaac
     6841 cctagaaaag cagcgcaagc cttacaaaaa gtagtacaag aaatggaaga acgatatgaa
     6901 aaattcgcgg caaccggagt aagaaatatt tctggttaca atgaatttgt tcaacagaaa
     6961 aacttggaaa acggaacgaa acatccaacc ttaccattca tcgtagtgat cgtagatgaa
     7021 ttagctgatt tgatgatggt agccagcaat gaagttgaag atgcgattat tcgtttagcg
     7081 caaatggctc gggcagcggg gattcatatg atcctagcta ctcaacgtcc aagtgtggat
     7141 gttatcacag gaatcatcaa agcaaatgta ccttccagaa tggcatttgc ggtatcaagc
     7201 ggtacagatt ctcggacgat cattgattct aatggggcag aaaagctctt agggcgagga
     7261 gatatgctat tcttgccaat gggtgagaat aaaccgatcc gtgtgcaagg agcattcatt
     7321 tctgaccacg aagtagaacg ggtcgttcaa tttgttacag atcagcaaga agctcattat
     7381 gaagagaaaa tgatgccgac agatgaagtt gaaacgacag gtgctccaga acagccacaa
     7441 gatgagctgt ttgaagaagc aaaagcactt gtcgttgaaa tgcaaacggc aagtatttcc
     7501 cttctccaaa gaagatttcg gatcggttac aaccgagcag cacgtttagt agatgaactg
     7561 gaagctcatg gagttgttgg cccgtcagaa ggaagcaagc ctcggaaagt attcattgag
     7621 caggaagaaa catcagaaac gttgccggac gataatgaca tgtaaaactt gtgtgatgaa
     7681 gggttgaaaa acgaataacc aataaaaaga caaggcatga tgataccaaa agaaaaaatg
     7741 gtttttttcc atcttttcta atacggtatg atgtcttgtc tttttagttt ttgtgagatt
     7801 aaagttctat tcgatttatt tgtgaagtat tgcattaagg aaatgaatca tgtatagtat
     7861 gtagcgaaac tttgagaaaa agagggaaaa tactttggaa aaacagtcca tttatggctt
     7921 gacgaatgaa gaattaatca attggtttat agaaaacggc gagaaaaaat tccgagctgc
     7981 ccaagtttgg gaatggttgt atcaaaaacg tgtaagtaat ttcacagaaa tgacgaatct
     8041 atcgaaacaa ttgatcgaaa aattatcgga gcattttatc atcaatcctc taaaacagat
     8101 ggtcgtccaa gaagcttcgg atggtacagt aaaatatttg tttgaactcc ctgataaaaa
     8161 catgattgag actgtactga tgagacaaga atatggcttg tcagtttgtg tgacgacaca
     8221 agtaggatgt aatatcggct gtacgttttg tgcaagcggg ctgctcaaaa agaaccgtga
     8281 tctgactgct ggagaaattg ttgcacagat catgatggtc caacattatt ttgatgagcg
     8341 caatcttggc gaacgcgttt cacatgtcgt tgtaatgggg atcggtgagc catttgataa
     8401 ttacgacaat gtaatggact ttttgcatat catcaatgat gccaaaggat tggcaattgg
     8461 tgctcggcac attacagttt ccactagtgg attagcacac aaaatcaagg aattcgctaa
     8521 taacggcttg caagtcaatc tggctatttc cttgcacgcg ccgaataatg aggtgcgaac
     8581 atcgatcatg cggatcaacc gaagtttccc aattgagaaa ttgatggaag ctgtggatga
     8641 gtatttagag aaaacaaacc gtcgaatcac gtttgaatat atcatgttga atcaagtaaa
     8701 tgatagacca gagcatgcgc aacaattagc agatctatta aaagataaga aaaaattagc
     8761 atatgtcaac ttgattccat ataatccagt cagtgaacat gatcaatatt ctcgcagtcc
     8821 aaaagctgat gtattgagat tttatgatgt attgaagaaa aacggcgtaa actgtgtgat
     8881 acgtaaagaa catggtacag atattgatgc agcttgcgga caattgcgaa gcaagcaaat
     8941 gaaaaaaaca gttgccaaat aatcgtatcc tactcgtttt ttcaattacc ataaaaggag
     9001 cagttttctt aattaaggaa ctgttcaact taaataagag aaaagattct atagcgaaag
     9061 ctataaagct ttttttgaca agaaaaaccc tcatcaagca aagaaatgtc ctcaatttga
     9121 tgacagcctg cttgatgggg gttttagcaa ttatttaact gattttctta tatccttgct
     9181 ggctttcttg tgtagcatga taagtttcta cgtcaagaat ggcctgttta aatttttctc
     9241 gggtgatttc ataaggcaca tgtttaatat catttgttgc catggcttta tctaagattg
     9301 cttctaaatc ggtcgtgaca ttgagattca tgtcggttaa cgatactgga agttggttct
     9361 ttttcataaa cacatagatt ttatcaaaca aatcgaactg ctgatccatc atcaataagg
     9421 tcagtacacc ataactgaca gatgctccgt gaaggtatcc ctccattttt tctagaacag
     9481 tcgtaccgta atagaatgag tgtgccatag cagaattata gttattgtta actaaaacag
     9541 aaaccaatcc ggtagtaaca atgatatccg aaacgacttg ttcaattgca tatgagggac
     9601 gatcgtcggc actatctttc aatgcttgcg taccataagt caagagacga tcaatgcaca
     9661 tttttccgat ttggattcct aatctatccg tgtagcctag atcatcgcca cgagcagaaa
     9721 aagtagattc gacttctttg gataaagcat cgccaatacc tgcccataag tatttatcag
     9781 gtgcttcggc aataattgct gtatcaatga aggtgtgtac aggtggtttt gaaggaaaga
     9841 agagtccgtc cattacgcct tcttcgttat agatgacaca taccgcagtg acgggcgaac
     9901 agttcgaacc aatagtggga aaggtgaaga ctggtttagc tagtttttca ccaatgactt
     9961 ttacagtatc gcaagcacgg ccaccaccga ctgcaaacag gacgtctgct ttttggacac
    10021 tttcatgctg gatcaactgt tcaatatttc cataagttga attacccccg taccagagac
    10081 tggcgacaac ctctatttcc tgtgtcaaag ctacttttat tttgttttct gcctttgaaa
    10141 gtgcagtctt ccctccaatc agcactactt ttgtcccata ttctttgacg attccatttg
    10201 ctttttcata cgcatctgtt ccaatggtat aatttggtaa gaaaacactt tgactcatat
    10261 tcatttcctt ctttcttcgt tgaaacgggt taaaatttta aagagtgatc gatctgactg
    10321 cggctgtaga tggagtagag gtgaacctaa gacaggataa gtgatgaata aggtggcatg
    10381 aacaccttga aatcatcaat aagtatccta aaaacgaaca agtacaaaaa cattgaaaac
    10441 gacaataatg aatttttgtt cctcttagta tttctaccga ttccctttgc ttttttgtaa
    10501 ttatctaaaa attctaaaaa ttaagattat tatatgaaaa caggttttat ttgtcaattc
    10561 ataattggtt ttttttaaag caaaagataa tctatatcat gatgaacaaa cagagttttt
    10621 tcctccaatt gatggctttc ctgatctttc ctattttgaa taaagtgttt ctgtataatt
    10681 tttttatttt ataagtaaga taaataatga aagaatagag atactgattg gtagctaaag
    10741 atttttgagc gagaacattt cgttttttca aaaggactgt tagaataagc aagaacaagg
    10801 gggaagacgt atgataatca gtgaatttga ccgtaataat ccagtattga aagatcagct
    10861 ttctgattta ctgagactga cttggccgga agaatatgga gacagctcgg cagaagaagt
    10921 agaagaaatg atgaatccag aacgaatcgc ggtagcagcg gtagaccaag atgagttagt
    10981 aggatttatt ggtgcaatcc ctcaatacgg tatcacaggt tgggaattgc atccattagt
    11041 tgtagaaagc tcccgacgaa agaaccaaat aggtactcga ttagtcaatt acttagaaaa
    11101 agaagtagct tccagaggag gaatcacgat ttatttaggt acggatgatt tagaccatgg
    11161 aacaacgtta agtcaaacgg acctgtatga gcatacattt gataaagtgg cttccatcca
    11221 gaaccttcgt gaacatccgt atgaattcta tgaaaaatta ggttataaaa tcgtaggtgt
    11281 cttaccaaat gcaaatggct gggacaaacc ggatatttgg atggcaaaaa cgattattcc
    11341 tcgaccaaat tctcaataat aaaaagaggt tgtgacagaa gtaatcagct tcaagaaata
    11401 agaaggaatt tacgaaaatt acttcttgaa tttttgtgaa attccaactt atttccgaag
    11461 aagctacttt tggaacaccg tgtaccagag gttgtgaaaa agtcgcttag tttcgagtag
    11521 taagaaaaat tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga
    11581 gaaactggct tttgaacacc gtttattcag ataagggact gtgacaagac ttttgtcaca
    11641 gtttcttttt gtttattctt ttttatcttt gtatggcaat aatagacaat ataacgaaaa
    11701 ttatttaatg gcttttggaa gaccatggaa aaccttgtac aaagctattt tttctgaaaa
    11761 attccttgaa acctcacgaa aaaatcatta ttttatgaaa aagacaaaaa aaagtgttga
    11821 ttttttaata attattcatt aaactcttaa acaacttcag aaaatattaa ttattctgaa
    11881 aattccaata aaggggtacg taaaatgaaa aagaaattta catttggttt ggttgcggta
    11941 tgcggccttg tacttgctgg atgttatggc ggcagttcag ccacaagcag tagttcagca
    12001 aaaagcggtt cagcagatgg aggaggcgta tttaacttag tcgtgcctca agaaatgcct
    12061 acagctgact tatcagttgc aacagataca atcagtttta cagcattgaa taacgtttat
    12121 gaaggaattt accgtcttga tgaagacagc aaaccacagc cggcaggagc tagcgagttg
    12181 gcagaagtca gtgaagacgg attaacttac aaaatcaaat tacgagaaga cgcaaaatgg
    12241 tcaaatggtg atcctgttac agcagcggat tacgtttatg gctggcaacg aacagtagac
    12301 ccagctacag cttctgaata tgcttacctg tatgcgccag ttgaaaatgc agaagacatt
    12361 acagctggaa agaaagataa atcagaacta gggatcaaag cagttggtga ctatgaatta
    12421 gaaatcaaat tgacgaaaca aacaccatat ttccaatatc tgcttgcttt cccatcattt
    12481 ttcccacaaa aacaatccgt agtggaagaa aatggggatg cctatgcatc tgcaagtgat
    12541 aaagctgttt ataatggacc attcacatta gaagattttg atggaccagg aacagataca
    12601 gaatggactt ataagaaaaa tgatcattac tgggacaaag atacagtaaa actttctgaa
    12661 atcaaagtca gtgtagtaaa agaatcttct actgcattga acttattcaa agatggccaa
    12721 gcagatgacg tgatcttgtc tggtgagtta gcacagcaaa acgcaaacga tccagcttat
    12781 acttctgtta aagaagcgcg gacaagctat atcgaattca atcaacgtga aaagaattca
    12841 ccattcaaca atgtgaacct aagaaaagct atttcttatt ctatcgatcg tgaagcttta
    12901 gtaaaacaag tactaggtga cggatcagtt gtctctacag gattgatccc atcagatatg
    12961 gtaaaaagtc caacaacgaa cgaagacttt gcaaaagaag ctggaaaatt agtttcttat
    13021 gacaaagata aagcaaaaga atattgggaa aaagcgaaaa aagaattggg cgttgattct
    13081 ttagaatttg atttgatggc ttctgatgat gattcatcga aaaaagtaat cgaatatgtt
    13141 caaaactcaa tccaagaaaa cttggatggc gtaacagtca aaccaacacc tgttccattc
    13201 tctgtacgtc ttgaccgctc aacttcgggt gattttgacg cagtactagg cggatgggct
    13261 gctgactatg cagacccaag cagctttact gatttgtttg tcacaggtaa ttcatacaac
    13321 cgtggtcgct ggagcaatgc agaatatgac gaagcagtta aagcagcttc atcaacagac
    13381 gctggcgatg aacaagcccg ttgggatgat ttccaaaaag caatcaagat catctctgat
    13441 gatatgggtg tcgttccggt gtaccaaaaa gcagaaggtc acctagtgaa tgaaaaaatc
    13501 aaaggtatcg ttcaccatgc ggcaggcgct tcatgggatt acaaatggac atacgttgaa
    13561 gaataatcgt taataccgtg agcatgagaa aaaacgaggc tgcggcaaat gtcgcaacct
    13621 cgttctcata ttcacttatc ctaacaaaga aaaaagcatg aaaatagcag aaagagcaat
    13681 tagtattccg gcaatgatca gccaaaagct gtacataccg tagctggcag acgagagcga
    13741 ggaaaattct ggttttttct ttcggttcct tgttcgtgca agatggaccg atcgctgaaa
    13801 atgatcgaag aaacccgaag aaaagaccca taaaaaaccg ccgataatca gaaacggcaa
    13861 tgaccataag aatagatcat tagataattg aatgagtgta aaggaatggt tgatgagatt
    13921 ttttattaaa ataaccagaa tcagtagaaa agctactgcg taggggatag attttttttt
    13981 catttgattg ctcctttatt tttggttcat tatctaataa tgtagcgaaa catctgtcag
    14041 aagtcaaaag ctagggggaa acattagtga atagttatat caaatatgta ttaaaacgtg
    14101 tctttttcat gatcatcacg ctttggttaa ttgcaacaat cacctttttc ttgatgcaat
    14161 tgctgccggg cacgccatat acgaatcagg aacgtttgag tcctgaaaca atcgagatgc
    14221 taaataaaca agtcggtttg gataaaccag ttatcgtaca atatgggatt tatctttcta
    14281 atttgcttca aggagatttt ggaatttcct tccaatttaa aaaccagcca gtcgcacatt
    14341 tgttagctgg aagaatcgga ccatccttgc agttagggtt acaggcaatt atctttggaa
    14401 cagtcctagg gacgatttta ggaacgatct ctgccatgaa gcaaaataca tgggcagata
    14461 catcttctac actcgtagcg atattaggtc gttccattcc aaactttgta tttgctgttt
    14521 tattacaata tatctttgca atcaaattac aagttttgcc aatcgcaaaa tgggatggct
    14581 ttgtgtacac aatcttacca acaattgcgt tggccatgtc gccgcttgct gactctgctc
    14641 ggtttatccg tacggaaatg gtggaagtac tgcatagtga ttatgtggaa ttagcaaaag
    14701 caaaaggatt gagccgttgg gaaatcgcct ttaaacatgg tcttcgaaat agtttgattc
    14761 cattgatgac tttattagga ccgttagctg tagcattgat gaccggatca ctggtggtag
    14821 aaaatatctt tgctattcct ggtatcgggg aacaattcgt taagtcaatc acaacaaatg
    14881 attatcctac gatcatggct gtaacgatcc tttactcatt tatgctgatt tttgttattt
    14941 tgatcgtgga tctattgtac ggtctagttg atccaagaat tcgtgtttca gaagggagcc
    15001 gaggataaat ggaaatgata ccaaagcgtt atgaaacagt tgcagatatc cctgcggatg
    15061 aatttcttcc attacaaaaa aataccgaag aagaacgtga acagattact gcaccttctt
    15121 tgaattttat ccaagattca tggcgccgtt taaagaaaaa caaagctgcc gttatttcaa
    15181 tggttttact attggtcatc attttgatct ctgtgatcac aatctttgtt tctcctcatg
    15241 atccaactgc ccaaaacgtg gcttatatca atttgcctcc tcgtattccg ggaatcaata
    15301 tcgatggatt gaatggaaaa gcaatggttg gtggtgaact tgttgataaa tatgctcaag
    15361 caaacgtccc ttcaaacgta aatttctttt taggaacaga cggtttggga cgtgatgtat
    15421 taagccgctt atttatggga atgagaatct cgttgctgat cgcatttatt gctgcattac
    15481 tagatgtaac gattggtgta gcttacgggt tggtttctgg tttgttaggc ggacgtgtcg
    15541 ataacgtgat gcaacgtttt ttggaaatct tatcaggaat tccaaacctc gttgtcatga
    15601 ttttgatgtt ggtcgttttt gaaccaggaa ttttttcaat cgtggctgct atggccatca
    15661 caaactggat accaatggca cgtatcgttc gtgcacaaac aatgaaatta aaagatcaag
    15721 aatatgtttt agctggattg acattaggtg aatcaagaat gaaaattgca ttcaaacata
    15781 ttttgccgaa tatctctagt gtcattatta ttcaaatgat gtttagtatt ccatctgcta
    15841 ttttcttcga agccttttta agctttatcg gtttaggtct tacgcctccg tcagcttcat
    15901 taggaacgat gttgagcgaa gggtacaaga cattcctgta tctgccttat ctattatgga
    15961 ttccggcagt aactttatcg gttatcatga ttggctttaa cttacttgct gatggattgc
    16021 gtgatgcctt tgatcctaaa atgaaagagt gaagacgatg acgaaaattt tagaagtgaa
    16081 agacttagaa atctcttttg atacgtatgc tggaaaggta cgagcgattc gcggtgtcaa
    16141 ttttcatttg aataaaggag aaacattggc gatcgttggg gaatctggaa gtggaaaatc
    16201 agtgacgacc agaagcatca tgcgcttgct gtcaagcaac gccaatatag atgctggtca
    16261 aatcctcttt aaaggtaacg atatcgttgg aaaatcagaa agagagatgc aaaaaatccg
    16321 tggtaaagaa atagcgatga ttttccaaga tccaatgacc tctttagatc cgacgatgcc
    16381 cattgggaag caagtcgctg agtcccttag aaagcataat aaagtttcaa aaaaagaggg
    16441 acaaaaagct gcattagaat tgcttaaatt agttggaata cccgaagctg agaaacgtat
    16501 caacagttat ccgcatcagt tctcaggagg tcagcgtcaa cggatcgtga tcgcgattgc
    16561 actggtatgt tatccggaaa tcctgattgc tgatgaacct acaacagcgc ttgatgtgac
    16621 tattcaagca cagattttgg agctgctgaa agaaattcag acaaaaatcg atacatcaat
    16681 tatctttatc actcatgacc ttggtgtagt agcgaatgtc gctgatcgtg tcgctgtcat
    16741 gtatggagga agaatcgttg aagttggaac atcggaagaa attttctaca acccgcaaca
    16801 tccctatact tggggactgc ttggttcaat gccgacatta gatagtgcga acgatcgatt
    16861 gtatgctatt cctggttctc caccggattt attgaacccg ccaaaaggag acgcattcta
    16921 tccaagaaat gaattcgcga tgaaaatcga tgcagaacaa gaaccaccat tttttgaact
    16981 atcaaaaaca cacaaggcag caacttggct acttgctccg caggcaccca aagtgacacc
    17041 gccagaagaa atccaacgtc gctgggctat ctttaaagag aaacaaaaac atacttacgg
    17101 tcaaggagga attgctgatg ccaaataaac tacttgaagt taaaggattg aaacagtatt
    17161 tcaatgtagg aaaaaagaat gaagtacatg ctgtaaatga catcagcttt cacatttatg
    17221 aaggagaaac attcggttta gttggagaat ctggaagcgg aaaatccact actggccgta
    17281 cgatcattcg cttgaatgaa cctactgatg gtgagatatt attcgacggt caagatataa
    17341 cgaagatcaa agataaaaaa ggtttgacga aattccggca tgatgttcag atgatctttc
    17401 aagatccata cgcttcgttg aatcctcgta tgaaagtcag agatatcatt gccgaaggaa
    17461 ttgatgttaa tggactagct aaatcatctg aagagcgtgc aaaaaaagtc gatgatctat
    17521 tgagaactgt tggattgaat ccaagccacg gtactcgtta tcctcatgaa ttttcaggtg
    17581 ggcaacgcca gcgtattggt attgcacgag cattggcagt aaaaccgcgt tttatcattt
    17641 gtgatgaacc aatttctgca ctagatgtgt cgatccaagc ccaagtggtc aatttgttgc
    17701 aagatctcca aaaagaacat caattgacct atctatttat tgcacatgat ttgtcgatgg
    17761 ttaaacatat tagtgaccga atcggtgtaa tgcataacgg cttgttatta gagatgggaa
    17821 caagtgatga aatctataat cacggtgttc atccttatac agaaagtttg ttatctgcta
    17881 ttcctctacc agatccagac catgagagaa aacgcagaag aatcaaatat caacctgaac
    17941 cagatgacgg acaagtacgt caacttcgtg aaatcgcacc agaacatttt gtctatgcaa
    18001 ctgagcagga agtcccatat tacgcaaaaa aactaaaacg acaaaaggaa tctcttctgg
    18061 ttgctaacta ggagggacat agatgctaaa agcttttaaa ttccgtatgt atccgacaga
    18121 agaacaaaaa cagcaattga ttcgtacttt tggatgtgct cgatttacct ataaccattt
    18181 gctgaaaaag cggcagaaaa gttggcaaca aacaggagta gccaattttt cgctcacacc
    18241 cgctacatta aaaaaagaat acccattctt aaaagaagta gacagtcttg ctttagcaaa
    18301 tgcacagctt aatttggatc gggcttttcg caattatttc aagggacgtg ccagcttccc
    18361 aaagcttaaa acgaaaaaaa gtatgtggca atcttatacg accaacaacc aaacccgtac
    18421 gatctatctg gaaaatggct acttaaagtt accaaaacaa aaagaactaa tcaagataaa
    18481 tcagcatcgg ccagttgaag gatcgattcg ctccgctacg atttctgctc gatataatga
    18541 agaattttat gtcgcactac tttgtgatgt ttcgccagta aaaaaagaat ctttagctaa
    18601 atggatcggg atcgcctatc atccgaagac attgatccaa acatctcgac ctctagaagt
    18661 aactttacca aaatttcacc agacagaaga aaagctgcaa catgcacagc ggaaattgaa
    18721 tgtgaaagtc cgctccgctc accatagaaa gatcaggtta gatcaggcaa gcaattatca
    18781 gaaacaaaag agaaaagtaa tggatcttta tctgaaacaa aagaaccagc gagaagatta
    18841 tcttgaacaa ttatctggga aattggtcaa acaatatgac tacttatttg tagaatcttt
    18901 tccaaaagaa gaggcacatg ctgatttttc tattcacgac tggcataaat taataacaaa
    18961 attgcgttat aagtcccaat ggtataataa aaaatttctt ttgatcaata ccgacggagc
    19021 agaagaaagc aattccgtaa gaaagagcca agtagtagaa aaaatgggca gacattcggt
    19081 tatcaaggga taaaaaatga atgaaactga taggagcgtt agggttagcc ttgttaataa
    19141 aaagtcttaa tgacttttgt ttctaggaat ttttcttttg aagaaaagct cactaattat
    19201 gacaaaccaa taaaacaata gcagaaaaag ttcgtacgag ctttttctgc tattgttaat
    19261 tttttattca gaaagtttac gaatacatca aaattctgaa acaaaaaacg taactcacaa
    19321 agagttgttt ttgcgttttt tcttgaatga ttaggtctga atcttttgct cacaacctta
    19381 ttcatggtgc cccggcttga gctgatagat ttttgaagga cggccgattc ctacaggacg
    19441 ttcaccgatc tcatcgaaat attggagcat agctttctta aaattggaat gatcgatcgt
    19501 tttataatcg atccctaaga atttagcaaa tacttttcga gcttccgtaa tcgtgaaatc
    19561 tttacctaaa acttgtagaa cttgcggctc gtgttccatt ttattgacta cacggttgaa
    19621 tgctttcaag atgatttgac tatggtcaaa agccaaagta tctttgccag aagactctcc
    19681 agtgactaaa tcaagcgaga tagccacttc tccgctagat aaatggatct tgttactatg
    19741 acgttctagt gtgaaccagc gtacttcctt ggcatcatct ccggcgatta gcggttcttc
    19801 tccaataaag gctaagtagc tgactgtgac gacccagcct cgcggatctc gattaggcgt
    19861 actgaatgta tgcagttgct caatattttc ttttgaaata accacaccag tttcttcctt
    19921 tgtttctctt aagacgcttt ctccagttga ctcatccttt tggacaaagc caccaggcaa
    19981 tgcccatgaa tttcgataag gatgcccttt tctttggatc aacaaaactt tcagctgatc
    20041 ttcttctttg ttatagcaca tcagtacgat atcgacagtc aaagagggct tgtcatattc
    20101 tggacgttcc tgtttttgat accagttaag aaattcttct tcacttgctt tatgttcata
    20161 atataatttt tcttcttctt tagaagcaaa acggttcatc tgaccacctc cattgacagt
    20221 aaaagtactt tttaccttat tgtagcagac tctgaaaaaa aaccaaggat ttacttgaaa
    20281 aatctaggtt atcatttaaa tatgaaaaat aaatggaagt atgagattga ataaaaagaa
    20341 aagcagataa aacggcaata ctttataccg ctcttcacga atggcaaaga agccacaaat
    20401 aacgatatat acgtcaatga taaaacgcaa aatccaagct gctgtcataa aactgttcaa
    20461 atgcccatgg aggttaaaat aaccatacat acgaatataa aaatagctga atattaaatg
    20521 tgctaatgaa agaaaagcaa tactaagtgt aaaaaaaaga tgtttcttct gcatgattgt
    20581 tccagtccct tactaagata gatagtcttt attataccac gtgtgagggg agaaataaaa
    20641 agccgttttt tagtagatat gaaacaaaac aaaaacccgc atttattcaa cctattcatc
    20701 cttttaaagg atagattgaa cgtagataga agtcgtttat ccagtctttt tcattttgaa
    20761 aaaaagctaa cagcatgctt tttttatggt atactagcaa ggaaacgaat tcgcatcatg
    20821 cgataaagga ggaagagccc cgtggcagaa aaggaaactt tttatatcac tacgccaatc
    20881 tattatccaa gcggcaaact tcatatcggc aactcgtata ctacgattgc atgtgacgca
    20941 attgctcgtt ataaacggtt gatgggcttt gatgtgtttt atctcactgg agtggatgaa
    21001 catggtcaaa aaatcgaaac aaaagcagaa gaactaggtg taaaaccgca agaatatgta
    21061 gataaaatgg cggctgatgt caagaaatta tggaaaacgc ttgatatcag ttatgataaa
    21121 ttcattcgca cgacagatga ttaccataaa gcagctgttc aaaaaatatt tgatcgttta
    21181 ttagaacagg gagatattta cttaggtgaa tatgaaggct ggtactctgt ttctgacgaa
    21241 gaattcttta cagaaacaca acttgcagaa gtgtacaaag atgacgaagg taacgtgatc
    21301 ggtggtaaag cgccaagtgg acacgaagta gaattggtaa aagaagaatc atacttcttc
    21361 cgcatgagca aatacgcaga ccgtctattg gaatactatg aagaacatcc cgaattcatc
    21421 cagccagaat ctcgtaaaaa cgaaatgatc aacaacttta tcaaaccagg attagaggat
    21481 cttgcagttt cacgaacaac ttttacttgg ggagtaccag tcaagagtga tccgaaacat
    21541 gtggtgtatg tatggatcga tgcattatcc aattatatta ctgcactagg atatggctcc
    21601 gaagatgaaa gtctattcga aaaatactgg cctgcagatg ttcagatggt cggtaaagaa
    21661 atcgttcgct ttcatacgat ctattggcca atcatgctga tggcccttga tttgcctcta
    21721 cctaaaaaag tatttgggca tggctggcta ttgatgaaag acggaaaaat gtctaaatca
    21781 aaaggaaatg tcgtttatcc tgaaatgctt gtggaacgtt atggtttaga tgcactccgt
    21841 tattatttgc ttcgtgctgt tccgtttggg tcagatggtg tctttacgcc agaagatttt
    21901 gtttcccgtg tcaattatga tctggcaaat gatctaggga acttattgaa tcgaacgatt
    21961 gctatgatca acaaatattg tgatggactc gttcctgact atgcctcact tgtcactcca
    22021 tttgatagtg aactgtctac gacggctgct aacgttgtcg gacgttacca tgatgcaatg
    22081 gaaaaaatgg aattcaatac agcattggct gaaatctggg tgctgatttc tcgtgcaaac
    22141 aaatacattg atgaaacaga accatgggta ttagcaaaag acgaagaaaa gaaagctgaa
    22201 ttgaatagtg tcatgatcca tttagctgaa tctttgcgta tcgcagcgat attacttcag
    22261 ccaatcatga cagaaacgcc agagaagatc ttcaatcaac taggattgga ctctgaaaca
    22321 atgaatcttg aaggattaca ctttggcgaa tttccttcag gaacaaaagt agttgcaaaa
    22381 ggtacaccaa tcttcccacg tttagatatg gaagaagaag ttgcttttat tcaagaaaaa
    22441 atgtctgaag gtactcaaac aaacgaagat acagtcaaat gggatccaga agaaacagaa
    22501 cttgtttcaa cgaaagaaaa acaaatcaaa tttgatgtat ttgaaaaagt agaactaaaa
    22561 gtagcagaag tcatcaactg tcaaaaagta gaaggggcag ataagttgct gcaattccga
    22621 ctagatgctg gagacagcca agaccgtcaa atcttgtcag gaatcgctga attttatcct
    22681 gatccaagcg aattgatcgg gaaaaaagtc gtcattgtag caaatctgaa accacgaaag
    22741 atgcgtggac aaatcagtca aggaatgatc ctttctgcag aagcaccaga tggctcttta
    22801 caagtaatcg aagcaccaaa atccatgcca aatggctcag aaatcgcata gaaattcaaa
    22861 gggggtatga cactagttgt gtcatacccc ctttttccga aatttgagaa gcaattttcg
    22921 taaattcctt cttattactt gaagccaatc atataataca gaaacagaga actatccaaa
    22981 ataaatccta gaaaaagaag tacgagcttc gctgattttt gttaatttag aataatacta
    23041 ataggaatcg taaaaacaga aaaaaagcat aatgataaag ggtcgggcag attttaattg
    23101 attcatctgg tactcttttt tattgtcctt acattcataa agtttgaatg caatgacttt
    23161 attacatcta tatttatatc taacagtagc gttgatgtgt taattataag ttctatcact
    23221 ttatgttcaa ttagtttgtg ctcttatatg tgtgattgaa aatatagata aaaaaacaat
    23281 catcggcttt gacttcgatg attgttttct ttattttgta aaaatcctat cacttgattt
    23341 tatggtaacc ttttattttt gtcttcaaac ggcttggtaa gagagcagta agcagtgcaa
    23401 atgggatcga aaagacatac cagaaaatcg tacgtccgat accataatgt tgatagagct
    23461 ttgtcaacat cacgtgtaac aagatcaacc ctaaaagcaa ggagccagct ataaaccaat
    23521 gattatccca atgcatgact tcactaagtg ccatcactcc aaaaggatag aaataaaaat
    23581 agatcccgac taaggtcatc cgccagttct ttgtaaatgt tccgatattc aataaaaaga
    23641 aaatcgctga aagcaaaata ccgataggcg gaaaaagccc aaccaaaaag gaatacaaca
    23701 tcccccacaa gatcatcaca ggacctttga tcaatgcaac gattgcgatg aacaaagtca
    23761 gcaatattag tttttgtgat aacgttgcaa cagtcattaa tccaaataaa attaatgtga
    23821 accaatgcca atgggactgc tgttgttctt tacgggaaaa atcgacccgg ttggataaat
    23881 aatctgctaa tttcggataa gaacgcttaa aatccataaa ctgttcctcc ttcgtggaaa
    23941 aggtaggttt agtatacgaa aaataaaaaa gaaatacatc cgtcttaaga gggagttttt
    24001 cactgaaagt ggagaaaaaa gaatggtaaa ttaaaatatt ctcagttata ctgaaaatta
    24061 taccaaattt caaagaagaa agaggatggg aatgaaaggc aaactaaaac gagaaatcaa
    24121 tctgtttggc gcattagcta ccgttatggg aaccgtgatc ggtgcaggcg tcttcttcaa
    24181 aactgctgct gttactgcta gtactcagtc tacaagtttg acattgctag cttggctttt
    24241 gggtggtttc ttaacgatct gtgctggatt gacagtggct gaattagcta cagcaatccc
    24301 ggaaactggt ggagctgtaa aatatattga agctgcgtat ggaaaattgc ccagcttttt
    24361 attaggatgg gcgcaaagct tgatttactt tcctgctaat attgctgcat tatcgattat
    24421 ctttgcgaca caattgacga acttattaca attatctacc gattatcttt tgttgattgc
    24481 agtgatcacc gctgtttctg tgacagggtt gaatttgtta gggacaaaag tcggtgcttc
    24541 tgtacaatca gtgacgttga ttgttaaatt aataccaatt gctgtgattg tcatatgggg
    24601 attattgacc ccaggacaag gaactgtcca gttatttcca atcgaagctg gaaaagatgt
    24661 tacttttgtc gaaggattga gcagtgcgtt gttagcaacg ttgtttgctt atgacggctg
    24721 gttaggtgtc ggtgcaatgg caggggaaat gaaacggccg gaaaaagatt tgcctaaagc
    24781 aattattctt ggtttgagtt ttgtaacagt cgtttatcta ttgatcaatt ttgttttttt
    24841 gaaaactttg ccgattgatc atcttgccgg taatttgaat gcagcttctg aagcatcaga
    24901 cgtcattttt ggaggaatcg gtggaaagct ggtcactatt gggatcttga tctcggtata
    24961 cggtgcattg aacggttata cactaactgg gatccgcgtg ccttatgcga tggctttaga
    25021 agacgagcta cctttcagta aacagctgac aaagctttca aagaagttta cggttcctta
    25081 tgttcctgcg gttttccagt tagcagtagc ttgtattatg atgagccttg gttctttcga
    25141 ctttttaaca gacatgttga tttttgtgat gtggctattc agtttactga tttttatcgg
    25201 tgtctttatt ttaagaaaaa aagcgccgga attgccacgt ccatataaag taccacttta
    25261 tccaatcgtt ccgattattg cgattctcgg tgctattttc attttaggta tgacgatgat
    25321 gacgcaaacg aaattagcat tgatcggtat aggtgttacc ttgataggta tcccagtcta
    25381 ttatcaaaaa aagaaaaaac gctcagaaga gtaaaatatt tcaacaacag acagaaaaag
    25441 tacgaactag gtcctctaga tcttcgtgct ttttctattt caataaaaca agttcttcta
    25501 ttttgatttt tgctacttct gcaagtagtg cttatgtgct aaagtaagaa tgaaaaggag
    25561 agataaacat gatatttgat tctcataccc atttaaatgc tgaacagttc aatgaagata
    25621 ttccagaaac gattcagcga gcacaagaaa tgggggtaac gaaaatggcg gtcgtgggct
    25681 tcgatacgcc aacgattgaa aagtctttgc aattaagcca tgactatcca aatatataca
    25741 gtattatcgg ctggcatccg acagaggctg gcagttatac gaaagaaatc gaaaataaat
    25801 tacaagaaca attgacttta ccaaaggttg tagcattagg ggaaattggc ttggattatc
    25861 attggatgga agacccgaaa gaagtccaag ataaagtatt tcgcagacag attgctatcg
    25921 caaaagaaat gaacctacca atcagtattc atactagaga agcacttgaa gacacgtatc
    25981 aaatcctaaa agaagaagat gttcgagata tcggtggaat catgcatagt ttcagtggtg
    26041 attatgaatg ggcaaaacga tttcttgact tagggatgca tatttcattt agcggtgtcg
    26101 tcactttcaa aaaagcatta gacgtccaag aggctgcagc aaatgtacca atcgaacgat
    26161 tacttgtgga aacagatgcc ccttatctgg caccagttcc ttttagagga aagcgaaatg
    26221 aaccaggcta tactcgttat acagtagaaa agattgctga acttcggaat cttccgacag
    26281 aagaagtggc tgcccaaaca tggacaaatg cgcaccgatt atttggtcta agcgaacatg
    26341 gataagatga agatccaaga gattatagtc gttgaaggaa aagacgatac aagaaggctg
    26401 cgggaagtag ttgatgcaga tacgatagaa acaatagggt ctgcaatcaa tgaagagatc
    26461 ctgatgcaaa ttgaacatgc ccaagagact cgtggagtga tcgtttttac cgatcctgat
    26521 ttttcaggag aaaaaataag aaaaatcatt atggaagctg ttcctcaggc aaaacatgct
    26581 tttttgccgc gcacacaagc taaaccaaag aaaaagggaa gccttggtgt cgagcatgca
    26641 agtgatgctg ctattttaga agcattgaaa aaggtcgtga cgccagacat ttcccaaacg
    26701 gctgtttctg aaatcactag acaagacctt ttagactatg gtttaatagc tggggcacaa
    26761 gcaaaagaaa aacgagaaaa actaggtgac gagttgcgca tcggttacac gaacggcaaa
    26821 caattggaaa aacgtttgaa gatgtttcgt attacgccga aagaactcgc ccaagcaatg
    26881 aaaatagtgg aggaacaaca tgagtgaata tcgtgaaatt gcgacaccct ctagaacaaa
    26941 agagatttta ttgaaacatg gtttttcatt taaaaaaagt ttaggacaaa attttctgac
    27001 tgaaccaaat attttaagga aaatcgtaga aactgcgcaa atagatgaac atacgaacgt
    27061 gatcgaagtt ggaccaggaa tcggtgcatt gacggaacaa ttagccaagc atgcaaaaca
    27121 agtcgtagca tttgagatcg atgatcgttt gatccctgta ttggctgata cgatgcaacc
    27181 ctatgacaat gtgactattg tacatcaaga tattttaaag accgatttgt caacagctgt
    27241 tagagaaaca tttcatgagg agcttccgtt aaaagtcgta gcgaatttac cttactatat
    27301 tacgacaccg atcatgatgc attttcttga atcagatttg gttgtggatg aactagtcgt
    27361 catgatgcaa aaagaagtag ctgatcgtat ctctgctgaa ccaggaacga aagcttatgg
    27421 ttcattatcg attgctgttc aatattatat ggaagccagt ttagcattca tcgtaccaaa
    27481 aacagtattt gtccctcagc caaatgtaga ttcagcgatt ttgaaactga caagaagaga
    27541 tataccggca gtcgaagtga cagacgaaaa agcatttttc cgattgacaa aagcggcatt
    27601 tcagcaaaga agaaaaacgc tttggaataa cttgcagcat tcgtatggca aagatgacca
    27661 gacaaaagca tggttagcaa aaagcttgga aacagctgga atcgatccaa aacgtcgtgg
    27721 ggagactttg tcattacaag aattcgccgc attaagcaat gcgatgtcag aaaatatgca
    27781 gtaaaaaaag aggttgtgac agaagttgtg aaaaagctgt cctgcatcaa gtaataagaa
    27841 cagccaaaca aaaatagttc ctcatgtttt tcgtttgttt gggcttgtta ccgcagatgc
    27901 aagcttttga acaccgttta agaaagaggt tgtgggcctg ccgtttaact ctaagtcaca
    27961 aggaacaatt tcaaaaaaca gcttctcatg tttttgagtt agttggtctt attacttgat
    28021 gcaagctttt gaccactatt atgccgaaca aggagatgtg acacaaattt tgtcacatct
    28081 cctatttttg tttcttgatc aaaaaattct tatacttgat actcacactc attttgtatg
    28141 gatttagaaa agaatatgtg tcttgacagt tattagtaag tgagttatac taagtcgaca
    28201 tttatgtgtc aagacatatt aggagagaga aaaaatgaga aaaaattcag ttcgaaaact
    28261 aacgatttct gctttattga ttgggatggg ggtcattatc ccaatggtca tgcctaaaat
    28321 catgattcca ccagcttcct ttactttggc gtctcatgtg ccattattta ttgcgatgtt
    28381 ttttacacca ggagttgcag tggctgttgc gctaggtacg acatttggtt tctttttaac
    28441 aacgccggtt atcatcgcat tgcgagcatt gtcacatctt gtgtttgcgt tgattggggc
    28501 ttggtacttg caaaagcatc cgagtatcgt tttaaaaaat ggacagttta cttttgctaa
    28561 ttggcgtttc caagggttca attttgtgat cggtgtgatt cattctttag cagaaatgct
    28621 agtagtcagc atcttttact ttattggtaa tatgcctgaa acatattatt ctcaaggata
    28681 tttttataca gtattcatct tgatggggat tggtgggctt atccacagtt tagttgatta
    28741 taatattgct tattttatag caggaacact cagcaaatat tttgatatcc ccgtatttac
    28801 ggcagcaaaa aaagaaaatc ggaaagagtc gattcaaggg tacgcgaaga aaacgacata
    28861 ttagacatga tctctcaaaa aaaaaactct catctagtaa tggataataa atcctctaag
    28921 atgagagttt tttgtcctaa aaagagctgc accaacatct tctggcacag ctctaacagg
    28981 gggcaattta tggaagtttg atgacttgtt ctgtttcttt atcaaagaag tggcctttgc
    29041 tgatgttgaa ggcaaggtcg actatttcgc ctggtttatg gaaatctctt gcatctactt
    29101 ttgagatgaa ctcagtagaa ccagtttttg tgtaaagcat cgtttcagct ccaagtaatt
    29161 cggaaacgac aacttctgat ttcactacgg cttcaggtga agcttcaagg gctacttgtt
    29221 cactgtggat gtcttctgga cgaataccga agataacttc ttttccttca taaccttttt
    29281 caactaatac tttgtttttt ccttcaggaa tacgaagctt aagcccatgt ccatctgtga
    29341 taacaccatt tttcaatgtc acattaaaga agttcatggc aggagatcca ataaagccgg
    29401 caacgaacat attgacgggt gtatcataaa cttctttcgg tgtaccgatt tgttggataa
    29461 atccgtcttt catgataacg attcggtcag ccatagtcat tgcttctgtc tgatcgtgag
    29521 taacatagat ggtcgttgtt tcaagacgct ggtgaagttt ggcaatctca gcacgcatgg
    29581 ctacacgtaa tttagcatct aaatttgata atggctcatc cattaagaag acttttgcat
    29641 cccgaacgat tgcccgtccc aatgccacac gttggcgttg tccaccagat aaagcagctg
    29701 gttttcgttt caagtattct gtcagaccta agatctctgc tgcattgtcc acacgttttt
    29761 tgatgtctgc tttatcatat ttacgtaatt tcaagccaaa agccatatta tcaaaaacag
    29821 tcatatgagg atacaatgcg tagttttgga aaaccatggc aatatcacgg tctttaggtg
    29881 ccacgtcatt catgactttt ccgccgatat ttagttctcc ttcagagata tcttccagtc
    29941 ccgcgatcat tcgcaaagta gttgactttc cacaaccgga aggaccaaca aaaacgataa
    30001 attcacggtc tttgattgat agattaaaat cagttacaga ataattttct gcattgtcat
    30061 attttttata tacgtttttc agagccattt ctaccataat acgttccaca ctttcttttt
    30121 tagttttatt aactatttaa agtataaatg aaagcgttaa ctaacttcaa tgtcatgttg
    30181 aacaagaatt ttttcttttt tttgtcaatg tgctagtttt aagctttaat aaatatccat
    30241 aagaaataaa aactgataca ctggagtaaa taagtatgtt aacaatatca aaaggagtgt
    30301 ctcttatgaa aatcgctttg atcgctcatg accgaaaaaa acctatgatg gtgaaattag
    30361 caacagcgta taaaacaata ttggaaaaac atgaactttt tgctaccggg acgactgggc
    30421 agaggatcac ggaagcaacc ggcttgccga tccatcggtt taaatccgga ccgttaggcg
    30481 gggatcagca aattggagcc atgatttcgg aggatcagtt agatttggtg atttttcttc
    30541 gagacccgct ggctgcacag ccccatgaac cagatgtaac agcacttatc cgtttatctg
    30601 atgtctatga gattccgtta gctacgaata tcggaacagc tgaggtgcta ctaagaggac
    30661 tggaagccgg cttcctagat tttcgaagaa tcgtccatga aatagacaag catccacttt
    30721 cattttgatg gataagcgta tggaaaaata acgtttaatg ttcgcctata tgtagctata
    30781 aaagctaaac aaactgataa tatgcgatat aatacgaacg tattggataa tataaacaat
    30841 aatattagtt tttcgtattt gcagtaaatg tgaaaatatg ctatagtttc tgtgggaaaa
    30901 gatcccaaaa ataatacaga gtagaaaata aagcgttaag tgctaagagg atgggatgtt
    30961 gcctcttgga cgaaggacgt aatgttcgcg gttttatttt cgcattcgct gcatgatacg
    31021 atgtagcagc tcttaaagga gatgctaaaa atgaagaaac accggttaga cgcacgtatg
    31081 attgctatta tgggcttatt gattgcattg atggtcactt tgtcccgact ggtagcaatc
    31141 gagaccccat ttattaaaat cagtgtcaca tttattccac aagtgatcat gggcattctt
    31201 tttggtcctt tttggtcagg gataggcgca gttttagctg atttagtagg aatggccctc
    31261 ttctcgaaat ctgcattctt tattggattt acattgaatg catttattga aggagcaatc
    31321 tacggattct ttttctatcg aaaagagatc acttggaaaa atgccattct ggctacgctt
    31381 agtgtcacgc tgatcatcaa cttatttttg acaccattat ggcttgcgtt gatgtaccat
    31441 gtgccattgt tcagctgggt cgtttgggcg ccccgcctgt taaaaacagt aatatggtta
    31501 ccgattcaat caattgccat ttattacgta ggacgtagta ttccttacaa aaaaatttta
    31561 agaagtttag cgatccacgc taaataaaaa acaagcagtc ttcccatccc agaagactgc
    31621 ttgtttttat tgtgctgtga attgtgcttg atagagccgt cggtaataac catctttttg
    31681 aagcagctca tcatgggtgc ccatttcgac aatagctccc tgatccatca ctaatatctg
    31741 gtccgcattt ttgattgtgg acaggcgatg ggcaatcaca aaactggttt ttccttccat
    31801 cattgctaag aaggcttgtt ggattttttt ctctgtcaat gtatcgactg agctggtagc
    31861 ttcatctaag atcaacatag ggggattact gatcatcgtt cgagcaatcg tcaacagttg
    31921 gcgttgtcca tctgatatct ttaacccttg accgccaatc ttagtatcta atttttccgg
    31981 caaacgttct acaaattcat acatataaga ggcttttagg gcatcattga tttctgcgtc
    32041 acttgcttcc ggattcccaa aacgaagatt ttctcgcaat gtactgtcaa atagccaagt
    32101 atcttgtaac accattccaa aacttttacg taatgtatca cgttggatag agcggatgtc
    32161 atggtcatcg actgtaattg ttccttttgt tgtgtcataa aaacgcatca gcaaattaac
    32221 aagtgtggat tttccggccc ctgtttttcc tactatagca atggtttgac ctggataagc
    32281 agtaaaattg aaatcttcga tcaaacgctg cttggggtca taagaaaaag aaacatgatc
    32341 aaaagagact tttcctttga tatttttggg ttccaaggca taggcatctg gcaaatcagg
    32401 cttttcttct ggctgatcga tcaaatcaaa tgctcgttct agaccagaaa aagcggtttg
    32461 cagttgtgtc gtgattccag aaagttcgat aaatggttta gaaaactgac ttgagtagat
    32521 cgtaaagctg gaaatcacac caacagtgat tgacgaacca ctcttcaatg ccaataggcc
    32581 gccaactaaa ccaatcgata aataagcaag gtgatctaca aaacgagaag acgggtttgt
    32641 caaggaagaa gaaaattgtg cccgttgtcc tttttcatat agatcagcat ttagcagttc
    32701 aaattgctgt tgactagctt cttcttgctg aaaagctttc acgattttct ggttaccgat
    32761 tcgttcggtc acaaagcccg agatatcccc aataatcctt tgctgtctat cgaaatttgt
    32821 ttgtgaagca cgagcaacga tccaactcac aaggaaaata attggtgtag aacaaagaac
    32881 gaccaatgtc agaagtggac ttagacgcat cataaaaata aacgaaagaa tgatgattgc
    32941 cactccagaa aataattgat tgaaggccgc agaaattgca atggaaatat tgtccatatc
    33001 atttgtaaat cgactgacga tatttccgtg ggagttttga tcataataac gaagtggcaa
    33061 tcgattcaga tgagcaaatg catcttttct taattttgca acggataagt aagctaaccg
    33121 attgcttaac acttggatga gccattgact gactacgttg attaaaacga taccaccgaa
    33181 tagaataagt aaatggaaca gtcgattgaa gcggacctgt ccttctccaa tcatttgatc
    33241 gatactttga ccaatttgta acgtcatcac aactgttgca atcccattga tgattcctag
    33301 tatcacagct agccaaatgt cttttgcata ggcagtaagg taaggaatga atcggcggag
    33361 cgtctgcaag gataattttt ttactttcat aactattctt cctcctgtga tgcgacgatt
    33421 tcctgatatt ctgctgattg agcaagtaat tcttcatggg taccctgagc agcaatctta
    33481 ccttgatcca tcactaagat atgatgtgct tcttgaatag agcggacacg ttgggagata
    33541 ataatcactg tactgccaag ataagtctgt agcgccttac gcaaatccaa gtctgtttga
    33601 taatccaacg cacttaaaga gtcatctaaa atcagtaatg ctggcttggc aatcaaagct
    33661 cttgcaatcg tcagtcgctg tttttgtccg ccagagaaat ttttcccatt ttccatgact
    33721 tttgtatcta aaccatttgg aagctgacgg acaaattctt ctgcctgagc gattttcaga
    33781 gcttcccaac attctttttc tgtggcgtct ttcttacccc attgaaggtt ttcacgaata
    33841 gtcccggtaa atagtacaga agtttgggga acaagagcaa tttgctgacg gagatgatcc
    33901 agctgccaag aacgaacatc cattccgtcc attataactg taccggaact gacatcatag
    33961 aaacgcggaa tcaattgtgt caaagtcgtt ttcccgcttc cggttggacc aatgattcca
    34021 aggactgtat ttcgcttcag ctgaaatgta atattttgga gagaaagacc agaatcagga
    34081 gtataacgga agtcaacatg gtcaaaagcg ataattgctt cagaagaaaa atcgggagta
    34141 gtactgacct cagcttcaag aatcgaattt tctgtgtcta gcacctcttt cacccgattt
    34201 cctgaagctt cggcacgtgt gaagatcacg acgaggtttg aaacaacgat cagtgccaaa
    34261 agcatttgat tcatataatt gatcaaagca agaacttgtc cttgttgcaa gctaccaata
    34321 tttactttga tcccgccaac tgtcagcaaa cagatgatcc cgacattcat gatcaaggtt
    34381 gtagcaggtg ataatagggc agagatattg gaaacacgta aataattgtt cgctagatca
    34441 tttgtgcttt tatcgaactt ctctgtttct ttttttgtct gcgcaaatgc gcggatgaca
    34501 cgaacaccgc taagattctg actaacgtgt tcattgagct gatctagttt ttcttgcact
    34561 ttttggtaga gaggaacggt ttttttgatg ataaaaaata aaatgattga gaaaataggc
    34621 aataatgcta agaaaaacag acctacttcc cagtcgatca caaaagccat gaccactgac
    34681 ccgatactta aaaacggtgc tctgatcaat aaacgaatga ccattgctaa agctagctga
    34741 agctgattga tgtcgtttgt cattcgggtg atcaaggtat ctgttccaaa atgattcaac
    34801 tcgttgtgag agaaagtatt gatttttttg attagctgat tacgtaactc tgtaccaaat
    34861 ccttgtgaag ctactgaagc atagtattgg caaatgatag cacaaatcag accaatcaca
    34921 gacatagcga ccatccataa gcccatttca atgatttttc ctcggtcatt catttttagt
    34981 ccattatcga tcaaggaagc catcattaaa ggcaggacaa gttcgaagac agcttcaaga
    35041 aatttgaaga acgggcccaa aatgatttgc ttacgatagt cttttgcata acgtaataac
    35101 tgaatcatat gattcctcct attatttatt tgtccacaat tgaaaaaaca agtactacca
    35161 cattgtagct taggaaattt agaatgaaaa gagaacaagc cttttcataa atgaaagttt
    35221 tattatgaag aagct
//
LOCUS       DARBDV010000015        35140 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000015 DARBDV010000000
VERSION     DARBDV010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.015"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            165..1310
                     /locus_tag="SCM56_000769"
     CDS             165..1310
                     /locus_tag="SCM56_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEG1109591.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            1447..1791
                     /locus_tag="SCM56_000770"
     CDS             1447..1791
                     /locus_tag="SCM56_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEG1109592.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            2062..3348
                     /gene="efmA"
                     /locus_tag="SCM56_000771"
     CDS             2062..3348
                     /gene="efmA"
                     /locus_tag="SCM56_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HEG1109593.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(3486..4085)
                     /locus_tag="SCM56_000772"
     CDS             complement(3486..4085)
                     /locus_tag="SCM56_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEG1109594.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            4489..5610
                     /gene="mnmA"
                     /locus_tag="SCM56_000773"
     CDS             4489..5610
                     /gene="mnmA"
                     /locus_tag="SCM56_000773"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEG1109595.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            5856..7277
                     /gene="arcD"
                     /locus_tag="SCM56_000774"
     CDS             5856..7277
                     /gene="arcD"
                     /locus_tag="SCM56_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HEG1109596.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            7291..8409
                     /locus_tag="SCM56_000775"
     CDS             7291..8409
                     /locus_tag="SCM56_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEG1109597.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(8469..9761)
                     /locus_tag="SCM56_000776"
     CDS             complement(8469..9761)
                     /locus_tag="SCM56_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEG1109598.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            9893..10471
                     /locus_tag="SCM56_000777"
     CDS             9893..10471
                     /locus_tag="SCM56_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1109599.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            10600..10977
                     /locus_tag="SCM56_000778"
     CDS             10600..10977
                     /locus_tag="SCM56_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEG1109600.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            10910..12982
                     /locus_tag="SCM56_000779"
     CDS             10910..12982
                     /locus_tag="SCM56_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEG1109601.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(13193..14140)
                     /locus_tag="SCM56_000780"
     CDS             complement(13193..14140)
                     /locus_tag="SCM56_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEG1109602.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            14386..14850
                     /locus_tag="SCM56_000781"
     CDS             14386..14850
                     /locus_tag="SCM56_000781"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEG1109603.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(14912..15955)
                     /locus_tag="SCM56_000782"
     CDS             complement(14912..15955)
                     /locus_tag="SCM56_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEG1109604.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(16332..17297)
                     /locus_tag="SCM56_000783"
     CDS             complement(16332..17297)
                     /locus_tag="SCM56_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEG1109605.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            17531..18376
                     /locus_tag="SCM56_000784"
     CDS             17531..18376
                     /locus_tag="SCM56_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG1109606.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            18567..19754
                     /locus_tag="SCM56_000785"
     CDS             18567..19754
                     /locus_tag="SCM56_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEG1109607.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            19760..20116
                     /locus_tag="SCM56_000786"
     CDS             19760..20116
                     /locus_tag="SCM56_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEG1109608.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            20118..20456
                     /locus_tag="SCM56_000787"
     CDS             20118..20456
                     /locus_tag="SCM56_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEG1109609.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    20532..20788
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            20851..21885
                     /locus_tag="SCM56_000788"
     CDS             20851..21885
                     /locus_tag="SCM56_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1109610.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            21878..22564
                     /locus_tag="SCM56_000789"
     CDS             21878..22564
                     /locus_tag="SCM56_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1109611.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            22588..23436
                     /locus_tag="SCM56_000790"
     CDS             22588..23436
                     /locus_tag="SCM56_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEG1109612.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            23633..24406
                     /gene="sufC"
                     /locus_tag="SCM56_000791"
     CDS             23633..24406
                     /gene="sufC"
                     /locus_tag="SCM56_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEG1109613.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            24419..25705
                     /gene="sufD"
                     /locus_tag="SCM56_000792"
     CDS             24419..25705
                     /gene="sufD"
                     /locus_tag="SCM56_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEG1109614.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            25702..26937
                     /locus_tag="SCM56_000793"
     CDS             25702..26937
                     /locus_tag="SCM56_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEG1109615.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            26924..27394
                     /locus_tag="SCM56_000794"
     CDS             26924..27394
                     /locus_tag="SCM56_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEG1109616.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            27399..28790
                     /gene="sufB"
                     /locus_tag="SCM56_000795"
     CDS             27399..28790
                     /gene="sufB"
                     /locus_tag="SCM56_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEG1109617.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(28885..30027)
                     /locus_tag="SCM56_000796"
     CDS             complement(28885..30027)
                     /locus_tag="SCM56_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1109618.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(30066..30800)
                     /locus_tag="SCM56_000797"
     CDS             complement(30066..30800)
                     /locus_tag="SCM56_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1109619.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            31032..31307
                     /locus_tag="SCM56_000798"
     CDS             31032..31307
                     /locus_tag="SCM56_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1109620.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            31405..33402
                     /locus_tag="SCM56_000799"
     CDS             31405..33402
                     /locus_tag="SCM56_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109621.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(33478..33813)
                     /locus_tag="SCM56_000800"
     CDS             complement(33478..33813)
                     /locus_tag="SCM56_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1109622.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            33940..34155
                     /locus_tag="SCM56_000801"
     CDS             33940..34155
                     /locus_tag="SCM56_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1109623.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            34192..34554
                     /locus_tag="SCM56_000802"
     CDS             34192..34554
                     /locus_tag="SCM56_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEG1109624.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            34600..34926
                     /locus_tag="SCM56_000803"
     CDS             34600..34926
                     /locus_tag="SCM56_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109625.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
BASE COUNT        11580 a         6221 c         7359 g         9980 t
ORIGIN      
        1 aaagaagttg tgacagaagt gagcagcttc aaggagctgc gacaagactt ttgtcacagc
       61 tccttttttc tatgcccatg aaacgtcctc tagacaaaaa tgagctcttc tttggtttgt
      121 tcgctatttt atgctagaat gtgcctagca ataatttgga agggatgaat gaagtgaaaa
      181 ctatttatct ggatcatgcg gcaactaccc ccatgcatcc acaagtaatt gaagcgatga
      241 cggcaatcat gcaagacacg tttggaaatc cttcaagcat acatggattt ggtcgccacg
      301 cgcatgaaaa attggaaaca gcaagacaaa tcattgcaga cagtctgcaa gcaaaacctc
      361 acgagatcat ctttaacagt gggggaacag aaggggataa tacagctatc ttagaaacag
      421 cgttctcgcg tcaaaaagaa ggcagacatc taatcactac agctatcgaa catcctgctg
      481 ttcttaatac catgaaatat ttagagacaa aggggtttga cgttacatat ctgcctgtag
      541 atgaaaaagg gaatctgtcg atcgaagagg tcaaaaaagc gttacgagaa gatacgatct
      601 tggtctctgt gatgtacggg aacaatgaaa tcggaaattt gatgccaatc aaagaaatcg
      661 gtgaactttt agcggatcat ccagcttatt tccatacaga tgcggtacaa gcgtacggaa
      721 accaaacgat ccatccagaa gaattaggaa tcgatttatt aagtatctct gctcataaaa
      781 tcaatgggcc aaaaggcgtc ggtttcttat ataaacgaga cggagtaaat atccctgcat
      841 tgcttttagg cggggagcag gaagaaaagc ggcgtgcagg tacagaaaat ttagcaggga
      901 tctggggtat ggcaaaagca gttgaactgc tgtcacctga agaacgtgaa gcacgtaaca
      961 acaaatataa aggattccag caattgattc ttgatacatt agaagcaaac gaaatccctt
     1021 atcaagtaaa tggtgattta acgaataaac tccctcatgt gttgaatctt cgcttaattg
     1081 gcgaagcaaa tgatttgcta ctgatgaagc ttgatcttaa tggggtcgct atttctactg
     1141 gttcagcttg tacagcagga aatatcgagc cttcacacgt attggaagca atgtatggag
     1201 aagatacacc gatactaaaa gaatccatcc gtatcagctt tggtttggga aataccgaag
     1261 acgacatcca gtatttcctt gatcaattag ttcgtgctgt caaaaaataa ctagtgaagt
     1321 cattcttata tgaacagatg tctgagagaa cgccttcatt ttttagggaa cttaactgaa
     1381 aaacctagta aaaatcagga taaactttta taataaagag aaactaattt tatcgaggtg
     1441 aaaatcatgg catttgaaac aacagcaacg gtactgggcg caccaattag ctatcgtttg
     1501 catcctgatg caaaaagata tacattgcgc gataatggat ttacagaaac aaacggaggc
     1561 aattatcaat tgatccgacc attagatgcc acgccgcaaa gcaaagaagg attcaaatta
     1621 aaaatcactg tatcaaaaga tattcaaaca ttgaaaatgt cgatcactac agcaaatgga
     1681 ttgaaagcag tcaatatttt caaagatcca aaacagaaaa tgagccaaga taaattttat
     1741 tttctgatgg atgggatgat tagccgtggg ttattcgaaa aagtcgaatg atctgtggat
     1801 ataaaaagac aactagtttt gaaacggcct attgttagat gactaacttt gtaaggacga
     1861 cgcaaattag ttgtcttttt tatttttaaa tttaacacat gttgttattt ttaatatttt
     1921 gctgatttat tgtttctaag taaagaaatt taaattgaaa cgattgttat ttagggaaaa
     1981 aggtattttt tgaaaaaaga atcccgtata atgattgttg tgcaaacgct atacaaaatg
     2041 aaaaagaaaa gagggatgtt tatggaaaat gaacagtcgg tcgttttaac gaattggaag
     2101 agaaattatt tgttcttttt atcgggacag tttttgtcag gaatcactag tatggtcgtc
     2161 caatatgcga tcatctggta tttgacaaga gaaaccggtt cggcgacaat tttgagtttt
     2221 gctactttac taggaatgat tcccatggtt ttactaagcc catttgtagg accgctagtg
     2281 gatcgctggg ataaaaaagc tcttttgatt gtcacggata ttattgtagc gattttcgcg
     2341 cttattttag cagtagtcgg aacgatttca gaatccttcc cgatttggct agtttttgtg
     2401 tctttgttta tgcgttcggt cgcacaaaca ttccaaatgc caacgatcca gtcgattatg
     2461 ccgacaatcg ttccatcttc tcacataaca aggacgaatg gacagttagg aatggtccaa
     2521 tcagcgaatt tcatcattgc gccagctcta ggtgcggctt tgttttcagt cgttcctgtc
     2581 aattacctta ttttattaga tgtattgggg gcagtttttg gtgtcggctt attgattttt
     2641 gtgaagatcc caaaagtttc tcctgaaata ttggaagttc cgcttactat ttttaaagat
     2701 gcaaaatttg ggctgcagca attgatggat aataaagggt tatggtacat aacgatcaat
     2761 ggtgcatttg tgatgctgtt gttcatgcca gcaatcagtc tgtatccatt gatgacgcta
     2821 gattactttg gtggttccgt tggacaggcg ggagcagtgg aagtagtcta cgcagtcgga
     2881 atgctcttag gcggtgcact gatcagtttt ataggaacat ggaaagaccg aatgaaacct
     2941 attattatag cctacatcat tatgggcctg acgatcggtg caagcggatt ggttccaaac
     3001 gatagtcaag ggttcttgta ctttcttatc ttgaatgcag gagcaggttg tgcaacgcca
     3061 tatttcaata cactgctgat ggcaatgatc cagcagagtt atgaatcgaa tgttttaggt
     3121 cgtgtcttag ggaacttcaa ctctttgatg aacctgacag gtccaattgg attattattt
     3181 gcaggaccat tagctgatcg tttaggggta gaaaaaatgt tcttgttttc cggaatcggg
     3241 attttattat gcggaatcgt tttattttta acgtcagctg cccgaaatta tgataaagaa
     3301 ttacagaaaa aactggtaaa agaacaccat gagcaaaaag acgaatagta tggaatggag
     3361 ttgtgacaga agtcttgtta caactccatt tctgatccta atgttcctgt ggcaataagc
     3421 ctactcaaat gaaaaatatg agtaattatc tttatttgtt taatacttga tatagggcag
     3481 ttttttcacg acctatcgta cttcctcttt gcttcataga tttctttgat ttgttgctca
     3541 gataacggag tgccaaatgg tagtttttcc tttaagagtt ctctttccgg tgcgtctacg
     3601 cctacattga catcgttctc gataggtacg ctgtaatgat ggatatgtcc atgaagattg
     3661 cggatagtgt cgttaggccc gagcaacatc ggataatgag tcaagtaata ttgttgatgg
     3721 tcgaatttaa cgatggcacc tacatcataa aaataaaatt tgggacttcc gtcagtaagc
     3781 ctaggatcat ttttttctaa ataacggaaa aaagcgcgac tgtcatggtt ccctttgatc
     3841 aagtggattg ttccttgcag ttgagtgacc agttccaaaa tttctgcatt tgctttttca
     3901 tattgcggat gcatagcaag gtcgcctaga tgataaacat ggtcggtttc ttttattact
     3961 gcattccagc tagtgatcaa ctgttggttc atttcatcca catttgcaaa gagccgcgga
     4021 gcaaagtcgt tttttcctaa taagcgttca tggaaaaagt gcatatcact gataaagtaa
     4081 ttcatgtatc ttttgcctcc cttttttctt agagtaaaca aaatcactta tctgagcaac
     4141 tcataacatc taaaaagaaa aggttgtaaa tgagacgtgc agctttaagc gataagaagc
     4201 aattttgaaa aatagttttt cacaatttat aaaattattc cttattgccg aaaggctggc
     4261 cctttttatg agtatgccaa ttttttatat cgaatgctca aaagatgagt gattttttgg
     4321 ttgactatca tgtttgtgta ctgaataggt ccagtagaac agtattttta cgacttgata
     4381 attaagggat gaaattttca gaaatatgac gtataatgta aaatactgaa aagttgcgac
     4441 cttcaactcg tagatgtttc agaatctaat tgaaatgatg gtgatatcat gacagacaac
     4501 agcaaaacac gcgttgtcgt agggatgagc ggcggtgtcg actcatctgt aacagcactt
     4561 ttgttaaagg aacaaggata cgacgtgatc ggaatattca tgaaaaattg ggatgacacg
     4621 gacgaaaacg gtgtatgcac ggccacggaa gattataagg atgtagcgaa agtagctgct
     4681 caaatcggga ttccttatta ctctgtgaat tttgaaaaag aatattggga tcgtgtattt
     4741 gaatatttcc tagccgaata ccgcgcagga cgtacaccta acccagacgt gatgtgcaac
     4801 aaagaaatca aattcaaagc attcttggat tatgcaatgg atcttggcgc cgattatgta
     4861 gctactggac attatgcgca agtcactcgc gatgaaaacg gcgtggtcca catgcttcgt
     4921 ggagtagaca ataataaaga tcaaacttac ttccttagcc aattatcaca agaacagctt
     4981 tcaaaaacga tgttccctct aggcggcatg gaaaaatcag aagtacgggc aattgctgaa
     5041 cgcgcaggcc ttgcgactgc taagaaaaaa gattcaacgg gaatctgctt tattggtgaa
     5101 aagaacttca aacaattttt gagcaactat ctgccagcaa aaaaaggaaa tatggtcact
     5161 cttgatggtg aagtcaaagg acaacatgct ggcttgatgt attatacgat tggtcaacgt
     5221 caaggactag ggatcggtgg cggaggagat tcgcaagaac cctggtttgt tgtaggaaaa
     5281 gatctagcga caaacacatt atatgtgggg caagggttcc atcatcctgc attgtatgcc
     5341 acaagcttgg atgcaagtga aatccacttt acgacgaatg aaccaatgcc aaaagaattc
     5401 aagtgtacag cgaaattccg ttatcgtcag caagacgttc ctgtgacagt tcgcttattg
     5461 gatgataatc gtgcagaagt cgtattcgac gaacctgtac gtgcaatcac tcctggacaa
     5521 gcagttgtat tttatgatgg aatggaatgt ctgggtggtg gtttgatcga ccacgcatat
     5581 caagaaacaa aagttttaca atacgtttaa gcgatattaa atgaatcaaa acagctatgg
     5641 ctaacaaaag aaacaccttt tcttttggat agcatagctg tttttttgca ataaaattaa
     5701 tttttattca ttttatactt gaaatacttg tgtaaaaagt tcataatatc caataatatg
     5761 aataaagttg ctttttgtta agcaagagca gttgcaaaat agaagggaat cttttgcaag
     5821 tgatttgttc aattttataa gtgagggtga tggaaatgga aaagaaatcg acgggtatta
     5881 gcaaatgggg actggttgct ctggttgtca gttcgtcaat cggaagcggt gtgtttggga
     5941 tcacaagtga tctggctagt tctgcagctc cagggccagc gatcctttca tggataatcg
     6001 ttgggattgg tattttagct ttggtattat cgctgaatca tttaggagaa aagcgtccag
     6061 atctagatgg tggtattttc ggttacgcgg aagcttcatt tggtaaatta ggtggcttca
     6121 tcagtggttg gggttactgg ttgtctgcgt ggctgggaaa tgttgctttt gcaacgatgc
     6181 tgatgagcgc catgggagaa tttttcccga tattcaaagg tggacaaaat gttccttcta
     6241 ttttatttgc aagtattttt atttggggtc tgactttatt agtaaacaat ggagttgaag
     6301 gagcgagcgt catcaataca atcgtgacga tctgtaaatt agtgccatta ttcctatttt
     6361 taatatttgt atttatcgca tttaaagtga atatattcac tgctgatttt tggggtaacg
     6421 tatctgataa tctagtgaat ggaacagggc agcaaggaac gatctggttg cagattaaag
     6481 gttgtttgat ggttatgatg tgggtctttg tagggatcga aggcgcaagt gttttagcca
     6541 accgcgcgaa gaaacgttca gaagcccagc aagcatcgat cattggtctg ctttgtttat
     6601 tgtttatcta tatccttgct tcgatcttgc catacggcgt cttatctcaa gaagagttag
     6661 caactatttc acaacctgct atggcaaata ttttaaaagg aatcgtggga aattggggag
     6721 cagcgctgat caatatcggc ttgattattt cgatcattgg ttcttggttg tcttggacga
     6781 tgctcccagc agaaacaacg atgttgatgg ctcgagacgg tctgttgccg aaaggttggg
     6841 gaaaaacgaa taaaaaaaat gccccaactt attcgttagt tgttactgca atcttgacta
     6901 atctattctt attgactttc ttagttactg attatgccta tcaatttgcc tattctttat
     6961 gtacagcggc aattttgatt tgttatctac ttgtcgggat ctatcaagtc cagttttctt
     7021 taaaagaagg aatcaaaaaa caagtagtga tcggtgggat tgctgtagtt tttgaattga
     7081 tgggcatcat tttagcagga ttttcttatg tccttttatg tagtattgcc tatcttcctg
     7141 gtttttgctt ctactggatc gcttgtcgtg aaaccaaaca tcaaattacg ggaaaagaaa
     7201 aagtaatgat tgggctgatt tgtgtggccg cactgctttc aatcgggttg cttgctgtag
     7261 gctggatcaa gatataaggg ggaaagaaac atgaaaatcg caaatttcgg tgtagaagaa
     7321 tggctaaata aatgggaaaa gaaagcggta tatgatattg cacaaagctc gatcgaagca
     7381 ttgacattag acgaactcgt tggcttagat gggactagcg taagtaaatt tttcgaaaaa
     7441 cgcaaaaacg aaccattgga ctatggctgg atcgaaggat cagatgtttt caaacaagaa
     7501 gtcgcttctc tttatcaaac cgtcgatccg gaaaacatct tacaaacgaa tggagcgact
     7561 ggtgctaatc ttttagctct atacgcgtta gtggaagcag gagaccatgt tgtttcgatg
     7621 cttcctactt atcagcaatt atatgacatc cccaaatctt taggtgccgc tgttgacttt
     7681 gttcatttga aggaagaaga agattggcag tttgatttag aacagctaga agccttagtc
     7741 aaacctgaaa caaaaatcat ctgtttgaat agtgcaaaca atcctacagg cacattgcta
     7801 gatcggaaaa ctttagaaaa aatcgcagaa attgctcgta cagtagatgc atatgtattg
     7861 atagatgaag tatatgcgcc tttaacggat aaaggtgaat tcttgtcgat cgtagatgtc
     7921 tatgaaaaag gtatagcaac caattcccta tccaaaacct actccattcc aggtattcgt
     7981 attggctgga cagctactgg acctgagttg gcagaagttt ttcgaaagta tcgagactac
     8041 acgatgattt gcggcggagt cctgtcagat gatcttgctg tacatgcatt gaagaataaa
     8101 gaaaaaatat tggcgcgaaa caaaaagatc attacagaga accttgtgat tttaaaacaa
     8161 tgggtagcca atgaaccgaa agttgaactt gtcgctccca attacgtctc tacttctttc
     8221 attaaattag ccatcgaaga agatgaccag acattttgta tcaatttatt agaagaaaca
     8281 ggagtactat tagtaccagg aagtgccttt gatttaccga agcatgcccg actaggttac
     8341 tgttgtaaaa aagaaacctt ggaaaaggga ttattcctct tatctgagtt cttgaaaaaa
     8401 caggcctaaa caaaaacact gcaaggacag tctgtgacaa ctgttcaagc agtgtttttc
     8461 atttacgctt attgtctatg agaaggagag tgccctgaag ctatggagac tcctgaagct
     8521 ttttcttctg cagtttcttg ctgatggatc tcttgtgtat tgtgtcgtac aagcaagaag
     8581 ttgaccaaaa caagaagaga agtcgaaaga aagacgccac gataaccaaa tgcactggaa
     8641 acactggagc cgatcatagg ccctactacg ttaccagttg cttggaaaga ttggttataa
     8701 ctgaagatcc gtccagcagc ttgatgaggc gaatattttg tgatcaatgc ttggacagca
     8761 gggagcagac aagcatcaga gataccaatc aaaaaacgta aggcagctaa ttgccaaaca
     8821 ttcgtgacga atgccattgg gatataaacc agaatcgcaa aacccaaacc aatcgcaagt
     8881 attctttcac tcccaatctt gtcacctaag cgaccaaatc ttggagcggc tatcaaagtg
     8941 gcaatcccag gtatcgaagc aatgattccg ctgatcaacg taacattccc atgtccatgc
     9001 agcaattggc gaatgtacag actgatgatt ggactaatgg aattgttgga tgcttggatg
     9061 atcatcgttg tgacgaacat accgatgacg acgtgtggat atttcaagtc tttgaaaatc
     9121 tgtttggcag aaaccatgtt ttcttttttc actgggacaa atttttcatg gacgaaaaac
     9181 agactcaaca agaagacgaa aaataagatg atccctgtaa tgaaaaaggt agggcgatag
     9241 ccaaacgctg aagcagagat cccgccaaga agcggtccaa gtaatgttcc tgtaacagaa
     9301 ccagttgcta aggttccaag aacttgacca cttttttctt ttggcgtccc agttgcgaca
     9361 agggcagtgg cattactgat ataaccagaa aaaatccctt gaagcagacg caatgcgact
     9421 aattgataca cactagtcac gcaacccatc aaagaaatga cgattgccat tcctaatgaa
     9481 gcacgcaaca gcatcaattt tcttcctttt tggtcggcta attttcccca ccaaggagag
     9541 ataagcgtag tcaccaagaa agtagatgaa aaagtcaatc cgctccaaaa gttcaattgt
     9601 gacgtactat aattacctaa cgtatctatg taaagtgaca taaaaggcat gactaaacta
     9661 aatccgatac cagccataaa tgttccaaac cataagacaa acaagttttg ttcccaagtt
     9721 tctcttttcc taaataattt ttcctttatt cttgcagcca attagactcc ttctttctga
     9781 aaattttcat ctacagctct ttattataaa ctacaatatg tagtttatca atgaaagtct
     9841 cttttttctc tttctttttc gtggtaaact gaaagagtga aggatttatt ttatgagagg
     9901 aaacatggaa atgaaaaaaa gaaatctgtc tcaagagaaa atcatcgact gctttcgaga
     9961 actagcagaa gagatggatg ttcagcaaat aacctttcag catttagcca aacgcttgga
    10021 tatcaaggct ccctcgctgt ataaccattt caaaaatatt cgtgatgtca aaacagcgct
    10081 aacagcgaaa cttctgcagg aacttaatga ccagctgcga cgggctcttg tgggaaaaag
    10141 tggtcaggaa gcactgcaga tctatgcaga aacttatcga tcctttgctt tttcaaatca
    10201 agcagtgtat gagttactga tcagtgtacc gcataccaat gaagctgttt tattagatgg
    10261 aatccatgaa acgaatcaga tcattttgca gatatttgag gcttttcaat taacgaaaga
    10321 agaaaaagtt catcgaagca gggaacttcg tagtatgatc cacggttatc tttccttacg
    10381 ttttttagga tactttacaa aagaaccaac aatcgatcca gaagacagct atttttggat
    10441 gatcaataac tttatcgcta ctttgcctta aacaatcaga ttcatttgta acaattttaa
    10501 gatgttcatt aaatgaccct tttataagag aaaattcttg caactttctt gaaaagtgac
    10561 taagatgagt ctagaaaagc tgaaagaagg agcacgagta tgtatcaagt aattaccatg
    10621 ttcggggaca atgagccttg gtggtttttt gaagactggg aagaagatat tgaagaagaa
    10681 gaaacattcg agtctttcga agaagcacga caagcgtacg aaaaacaatg gaacgaaatc
    10741 catcaaaatt atgaatatat caatgcaaaa agtaactttc tcagcgcctt ttggaatgac
    10801 ggagatgagc gctggtgtga agagtgtgac gatgatttac agcaatacaa gggacttgcg
    10861 ttgttgaaaa atcatcagcc catcacagta gaaagcagaa aggaatttta tgaaacaact
    10921 aattctagcg gaaaaaccaa gcgttgcgaa agacctaagc aaggtgcttg gtgctaatca
    10981 aaagcacaaa aattattatg aaggacccaa ggtgatcgtt acttgggccc ttggtcattt
    11041 attaggattg aaaatgccgg aagatctgaa taaggaatgg cagagctggc aaatggacac
    11101 gttgccaatg atccctaaaa aactaggaat caaaccactt cctaaaacag gacatcaatt
    11161 aaaagcaatc aagcagctgg ctcagcgaaa agatgtctca gaagcagtca ttgcgacaga
    11221 tgccggaaga gaaggggaac ttgttgctcg ctggatctta gagtatgtgc atttcaataa
    11281 accagtgaag cggttatgga tctcttctca aacagataaa gcaatcaaaa ctggattcaa
    11341 gcaactaaga cctgcgaaag aatatgatgc attgtatgaa tctgctcttg cccgggcaaa
    11401 agcggactgg ctggtaggat tgaatgtgac cagagcctta actgtgaagt acgcggataa
    11461 tttatcagca ggccgtgtgc agacaccgac actagcatta gtgagagacc aagaaaagaa
    11521 aatcgagcag ttccgtccac aaacgtattt cacgatcacg ttgcaagctg gaaaagaaca
    11581 agccaaaatg cttcaaaaaa atcaatttgc tttaaaaaca caagaagaag cacaaaaact
    11641 tgttcaattg atcagtacaa gcaaaggaac tgtcacagaa gtccaagaaa aattaaaaac
    11701 ggaatctgcg ccattgcctt atgatttaac agagatccaa agagaagcca atcaacgcta
    11761 tggctattct gcaaagaaaa cattaagtct tgtacaaagt ttgtatgaga cccataagat
    11821 cgttacttat ccgcgtacag acagcaaata tctgacaaat gacatgaaaa gtacgatgaa
    11881 agaacgattg caagcagtca gcgactttgc tcctgaggtc aagcagtatt tgaaaaacgg
    11941 agccatcgtc caacagacaa aagtattcca agacagtaaa gtgaccgatc accatgcact
    12001 tttgccaact gaaaacagag cacgttatga aaaattaagt aatgaagagc gaaaaatcta
    12061 tcaaatgatt gtctctcgtt ttttaggact gtttgctcaa ccccataaag tcagccaaac
    12121 aaaagttacg gttgaatttg gtaaagaacg attcattttt cggcaaaatc gtgtcgtcca
    12181 agcaggatgg aaaggcgaaa ctgaaactga gacggaaaca gtcaaatggg aaaaaggaat
    12241 gagaatcaat cccgatttcg ccatcaaaaa agaactatct gcaccgccta aaccattgtc
    12301 agaagcaagc ttgcttggtc aaatggaaaa atacagtctg ggaaccccag ctacgcgtgc
    12361 agagattatc gagaaactaa tcaaatctga actgatggaa cgtacccctc aaggactgca
    12421 agtatctcct aaaggtaagc aattattagt attggtaaat ccctctttag taacaccaga
    12481 tttgacagaa agctgggaaa aagaccttga agctattgct gcaggaaaga aacaagcagg
    12541 aaccttcttg aatggaatcg aaaaagaaac aaaacgcttg gtgaatgaga tcaaatcaag
    12601 caaacaagaa tatcaagatt tctcgattac ccaaaagaaa tgcccagaat gtggggccaa
    12661 tctgcgcgag aaaaacacac gtgacggcaa aatctatgtc tgcaccaatt cagattgcaa
    12721 ctaccgcaga agaaaagatc caaaagtctc taatcatcga tgcccgcaat gtcaccggaa
    12781 aatggaaatc atcgaaggta aaaacggtgc ttatttccgt tgtaaatacg atggaacgac
    12841 agaaaaattg cttgataaaa aagagcagaa gaagaaaatg accaaacatg aagaacgacg
    12901 cttgctgaaa aaatatgcac aagcagaaga accagaagaa agcccattag cagcagcact
    12961 aaaagcagca atgaagaagt aataagatat gaaagaggtt gtgatagaag tggtcagctt
    13021 caagaaataa gaaggaattt acgaaaattg cttctcaaat ttttgtgaaa tttcggctta
    13081 tttccgaaga agctacttct ggaacaccat ttattcggaa aaaaggactg tgacaagact
    13141 tttgtcacag tcctttttca tttaaaaaaa tgtttacttc atttgacgta tattattggt
    13201 gaggcttcaa ttcatttggc ttcacttttt ctgtttctct tatgatataa acaggacgct
    13261 gtttcgtttc taagaagatt ttcccaatat atttcccgat gattccaaga cataacaatt
    13321 ggattccacc aatgaacagg aagatcgaga ccatggaagg ccatcctgat gtaggatcac
    13381 caaacaacaa tgcccgcaaa ataatgaaca aaattgcaat accagaaccg acacaagaca
    13441 aagcgccgat aaatgaagca atcgttaacg gtgcatccga gaagttcacg ataccatcaa
    13501 tcgagtagtt gaataatttc cagaaggacc aagatgtttc gccagccaca cgttcgcggt
    13561 tttcataaga aatatattct gttttaaatc cgacccagct aaataatcct tttgaaaatc
    13621 ggttgtattc tgttaattct aggacggcat ccaccatttg acgagtcatc aagcggaaat
    13681 cacgagcgcc atcgaccatt tctgtatcgc tgattttatt gatcaactgg tagaattttt
    13741 tcgcaaagaa actgcgtatc gcaggctcgc cttcacgagt cgaacgccgg gtgccgacac
    13801 aatcgatatc agaagtagtg atccggttga tcatctcagg aagcagttcg ggcggatctt
    13861 gcagatcaac atccatcacg gtcactaaat ctcctgtaga agcttgcaat ccagcgtaca
    13921 gagccgcttc tttcccaaaa tttctggaaa atgagacaaa gcggacataa ggataagctg
    13981 aggctagctt cttgattgct gctaacgtac cgtcttttga tccgtcgtca ataaacaaat
    14041 attcgaactc atgggcacct ttgaattttt caacctcatc aaaaaaaaga ggaacagttt
    14101 tttcttcatt aaaacatggg acaacaattg atactttcat cgtttctatc ctttcaatcg
    14161 tatgtttata tatggtttac attcaaaatt taataaacct taaataacac ctttaaaatt
    14221 tgattttacg taaaagaaca ggtattcttt ttgtaaacta acgtatttag tttaccatgt
    14281 ttcctcggtt tatcaaactt ttaagaacat tttttattag ttggcaagta gttttggata
    14341 gagaaaaagg aggaagttcc agtctattga ctaggagcaa acaaaatgaa aatacacaca
    14401 cctatcggct ttttatggtt agatgcagat gaatccggat tgactgctgt atcttttcag
    14461 ccaatttcag aaagcgaatc agcgaatcag cgaattttaa cgcgcacaca agaacagttt
    14521 gaagaatatt ttgcgggaaa aagaaaagaa tttgatgttc ctttatctat aaagagaggt
    14581 acgccttttc agcaaaatgt ctggaaagct ttgtcgacca ttccttatgg agaaatccga
    14641 acgtataaag aagtagccgt tgctgttgat agtccgaaag ctgtgcgagc gattgggcag
    14701 gcaaatcgaa ccaatccatt gccaatcgtt attccttgtc atcgagtgat cgggcaaaat
    14761 ggaaaactga caggatatat gggaaacgca atagatgggg ttgaaatcaa acgccaattg
    14821 ttgttgttag aaggatatct gcaaaattaa aaacagttgt gattcggaca ttgttccatg
    14881 cgagaatcac aactgttttt tcatgttcat tctaaacttt gatcagccaa ataaatagta
    14941 aagccatcac tgtttggaga agcccgacag ctaatccata agataagaag cgtgtacctt
    15001 ctttcatgaa ttttgcaaag tctaaacgca gaccaattgc cgctaaagca gtgatctcaa
    15061 accagctgct gataaaatgt gccgattggt caataactac aggtaaagaa aacaagctgt
    15121 tgaccaaaca aaagatcaca aaccctaata cataccaagg aagcggtaat tttcgttttt
    15181 tctgcgtatc agttggcgtt tcaaacagag ctggttgttt cttgtgcatg aatttttcaa
    15241 acagatagac aacaacaacg agcatcataa tacgtagaat cttaaatagc atggcgaatt
    15301 cgacaacaga attgtcaagt aagcttgccc cagcgactac ttgaccaaca gattggagtg
    15361 taccacccaa aagtgcactt ttttcaatga gatttccacc aaacaactgt aatccaagaa
    15421 aagggagagt caacatcatc acagtaccca gtagattgac tagtgtgatg atctgtcctt
    15481 tttcttcctc tttcgcatgg atggaagggg cgattgaagc aatagcagaa gaaccacaaa
    15541 cagcgtttcc tccagccatc atcaaagaag aagtatcgct gaattgcagt tttttaccaa
    15601 tcaagtaagc accgatgata gtgactgaca tttgcagtaa cacaaaaaca attcctttta
    15661 caccaatttg agcaatcgtt tgagctgtga cagttgcacc tagtaaaaaa acagagaatt
    15721 ctaatagttt gctttcagca aattttgtgc cgattgctag aacaggctgt ttaaaaaaag
    15781 tatttcctaa taaaataccc aataaaatag caatagtcgc tccacctaac tgtggcagat
    15841 aaatagcaag aagcctgctg aaggcagcga tggcaaaaga aagcagtaaa ccgggtaaga
    15901 tttcgcggac cgctgaatcc tttacagatt ttattggatg tgacaatgat ttcatttgat
    15961 cggttccttt cgtcaataaa tactaaaagc atactcgaac atagtagcac taaaccagta
    16021 ttttgacaaa agaacaagag gtcgtgacaa aagtcttgta cgacctcttg tttcgaataa
    16081 acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa atttgagaag
    16141 caattttcgt aaattccttc ttatttcttg aagctgacca cttcttttac gacctcttgg
    16201 taaacggtgt tcaaaagctt gcatctgcgg taataagccc aaccagtaca aaaacatgaa
    16261 cagctgtttt tgtttggccg ttcttattac ttgatgcaga acagcttttt cacaacctat
    16321 tgaatcgatt attaagcgat ttttttagaa acaaattttc catgtttgct atgttcttta
    16381 attaagaaac gtacgtaatg agcaagaacg tagaagacaa ctacacttgc aaacagacat
    16441 tccgcaactt ttaagaaatc caatacgcta ctatagatac ctaaactgtt gaagtaatga
    16501 acagtagtaa atatagctgt tgcagagata acaagcggga atgtaaaggc tgcatagctt
    16561 ggataaaaac gaagcttgaa caaacgtgga agcatagaca gcacaacgaa atacaatact
    16621 tgagataaag cgaacaatgc tacgacaaga taaaaattag gttcagcaaa gtttttaaga
    16681 taacctgtta ggcaaagaga accaggtgct gcaacgatcg taatcagcgg acgtgtcgga
    16741 tgagccatat ttcttacgaa aaagacacgt ttgatgatga tcggcaataa gaacgcatag
    16801 cagatcaatg aaatccaaaa gaaaattttt ccaataagtg ggaagaagtt cccggcagtt
    16861 acagtgatga ccccaaatcc aacgtaaatg ataaaccagc ttggataaat tgcttcaatt
    16921 gaaagatcag ctttgataac atggtgataa ttgaaataaa agacaaggat cacttggaag
    16981 ataacagcgc acagccaaat gaatttgaca attggtgcaa tactttcata tgaaacaaaa
    17041 taagtacaaa taaccatgag tgacatcgta aaagttggtg aaacggaggc gatgattgga
    17101 tcatgcaacg tttgtttcgt gtgttcgaat aagataaaca atttaacaaa aatgaatatc
    17161 ataagaatca tagagatacc gccagttaga ttgcctaaag caacatggtg atagtcttta
    17221 aacagatttc caagggaagc taacccaagg atcaacccgc agatcgggat cggaataatt
    17281 ttcaaaaagt tcttcatata taatattctc ctttttattt aatagatttt agtaccaaga
    17341 tgaacgaata cattgtaaaa tgaataggaa tgtatgatta cgagcaagca gacagctgat
    17401 tgtcttcatc taagttacta gttggatcag caactgtttt gttgtacatt tctattatag
    17461 atggtttttt gtatatttaa atcaaatagt ttttatagta attatcataa aaagttatgg
    17521 aagtggtgcc atgtttaagt tattaacgac ttttcgagca gtatatgaaa caagaaattt
    17581 ttccagagca gcagaagtac tgtttttatc tcaaccagca gtctcgaatc aaatcaaaca
    17641 gttggagcgg gaattgaata tcgatctttt tcaacgtaac ggaagaaaag agatgcttcc
    17701 aactaagcaa gccgacttat tatatgaacg gacactggtt ttgttagaag aatgggaaga
    17761 aactcagcaa aaattgctca atgaacaaaa tgaagaagaa gtatgtcgga tcgttgcttc
    17821 ccacaccttt gctgtctatc ttttgcctca gttgatgaaa catctgatca aaaggtttcc
    17881 taaagtgcat tttgagatcg aaatcacgaa ttctcatgaa tcattagaac aagtagcgaa
    17941 acatgaatcc gattttggtt ttatcgaaaa gccattagag acttccggcg tacaaagata
    18001 ctctttgatg gaagaccagc tagtcatcgc tggggaccca aaaagcaaat tgtggcttgt
    18061 aagagaagca aactctggtg tttttcatta cacgcagcgt tattttgaag agatgaatat
    18121 ccaagataag aagatgatcg tgaaaagtaa cgatgtaatt gttgcgttgc tgaaggaagg
    18181 aatcggtcgc tcgattttgt ctaagcgctc tgttccagaa aatgtgccaa tgactccgtt
    18241 gaatttccat cgatattttt atttcatcca acgaaccaat ctggtttctg atcagctgct
    18301 agagattgcc gaagagatcc ttcgatacaa tgaaagcaca gaaacttctc acagcgagga
    18361 gaatctcagc tggtagataa ttgcatgtat caaaaaaagt tctatgatac actaaagata
    18421 agcagtaggc ttttgtactg catttgctca agcaactgca caaacggaaa gttaattttt
    18481 cccaaatgtc tatggaaaga ctttccatta tctaactgga ccgctataat aatcagcagt
    18541 atggttttta agaaaaggga tgaaacatgg aaaaaaaatt aaaagcttcg aacgataatc
    18601 gtattgatta tggcgtgatt ttaccagtct ttctgttatg tttgattggt ctgctctcat
    18661 tatatgtagc acttagccat gatccgaaca atccagcagt cttgaaaggc gtagggatgc
    18721 aagttgtctg gtatttagtt ggagcggcag caatcgttgt catcatgcat ttgaattcca
    18781 aatggatctg gaaactgact ccttaccttt atggtttggg gttagtggta atgggactct
    18841 tgctcaaatt ctatgatcag aatttagctg cttcaacagg gtctaaaaac tggtttcgat
    18901 tcggtgcgtt tactttccag ccagcagagt tgatgaagat tgcgtacatc ttgatgatgg
    18961 cactgatcgt aactaaacat aatacgcaag taaaagaacg gacgatgaaa tcggactttt
    19021 ggctgattgg gaagctcttg atcgtgacaa taccggtttt agcattgatc atggcccaag
    19081 atgactttgg aacgatgctt gtctttcttg cgatctttgg cgggattttc ctgatgtctg
    19141 gaatctcttg gcgcatcatc gcacctgtag taatcttggc agtagtcgtt ggagcaggaa
    19201 cgatcttctt ggttacgact gaaggcggaa gagacttgct ttataaagtt ggattcaaat
    19261 cttatcagtt tgcacgtatt gattcatggc tggacccttt ccatgatacg tcaggaatga
    19321 gttatcagcc ggcccaagga cttttggcaa ttggtaccgg tggcttgttt ggtaaaggat
    19381 tcaacgtaag caatatctat gtgcctgttc gtgagtccga tatgattttt accgttatcg
    19441 gtgaaaactt cggctttatc ggtggtgcat tcgtcatttt cttgtatttt attttaatct
    19501 atcgtatgat tcgggtgtgt ttcgatacga ataatgaatt ttacgcctat atcgcaagcg
    19561 gtttgatcat gatgctgctg ttccatgtct ttgagaatat tggagcaaat atcggattac
    19621 ttcctttaac agggatccca ttgcctttca ttagtcaggg aggatcctcg attttaggga
    19681 acatgatcgg tatcgggttg atattatcga tgagatacca aaatgaagca cctgcaagat
    19741 cttcaaggag gtaagaaaat tgtacacctt ttactggtac ccgaaatgtt caacatgcaa
    19801 aaaagccaaa gcgtggttag accagcatgg cgttagttat caaaccgttg atatgatcaa
    19861 agaagtaccg aaggcctcaa cgattgagac ttggctagaa acgagtcaag taccaatccg
    19921 tcgcctgttc aacacaagcg ggatgaaata tcgggaactt ggactgaaag atcaagtagg
    19981 cgagtttacg gcttcaaaag cgagccaagt cttatcgaca gatggtatgc tgatcaagcg
    20041 accactgatc ttacgagagg accagcttgt tgcaattgga ttcaatgaaa aaatttatga
    20101 aggagaactg atataaaatg gcaggcaagc atttgaagaa aaaggacaac ctttggatct
    20161 tatttaatgg aaaagaatac tgtgttggtt tgacaaatga agcgcaagaa gaacttggag
    20221 acatcacgtt tgccaatctg ccaaaagcag gacaatcgct aaaaaaagat gaacctctga
    20281 tcgaagttga agcggaaaaa gcggtcaatg aatttgccag cccgctaacg ggtacagttt
    20341 cttctgttaa tgataaaatc ggtgaagata tcaatatctt gaatgattca gatgaaatga
    20401 atgcgtggat cttaagtttc aaagatgttg atccaaaaga atttgacgaa ttataaaatc
    20461 agaaaatcac ttgaaaaaag ttgacactga ttcttccagt tgctataatc ataagcaatc
    20521 ctatatgaaa cgagaaagct ttgaaaagaa gagtacatgt cacggacgtt tgaagagagc
    20581 cctgtttgct gaaaatgggt aacgaaaatg agatggaaga tggtcttaga gcagagtaag
    20641 cgagcgtaag caaacttact acgggagtgc ccgttacagc gcgacagtat acggaaagaa
    20701 gctaagtcca agtactggaa gaggctgtta ttgcgaagta acggtaaatc aaggtggtaa
    20761 cacggaagtt caagctttcg tccttttgct taaggcaaga gggcgaaggc ttttttgttt
    20821 gaaaaatcat aagagaaaga tgtgaatggc atgtcattaa tccaactaac gaatgtcaaa
    20881 aagacattca atgggaaaaa cggaaacatc caagccgtca atgacgtttc actaaacgtt
    20941 gaaaaagggg atatttatgg aatcgtcggc tattctggcg caggaaaaag tacattagtc
    21001 cgtctcctga atggacttga gcttcccacg agcggcgaag tgatcgtaaa cgatcaagat
    21061 attacaaaat taaaaaacaa agaattacgt acattccgaa agaaaatcgg tatgatcttc
    21121 cagcatttca acctgttatg gtcaagaact gtacttgaaa atatccagct gccgctggaa
    21181 ttagctggcg taccaaagag caaacgtaaa gaacgggcag aagaactgtt gcgtttagtc
    21241 gggttagaag gacgcggaca agcatatcct tcacaacttt ccggcggaca gaaacaacgc
    21301 gtcggtatcg cacgagcatt ggcaaatgat cctgagatcc tgctttgtga tgaggctact
    21361 agcgcattgg acccgcaaac aacagaagaa gtattagact tattgttagc gatcaataag
    21421 aaattgaatt tgaccatcgt cttgatcaca catgaaatga atgtgatccg caaaatctgc
    21481 aacaaagtcg ctgtgatgga gttggggaaa gtcgtagaag aaggagacgt attgactgtc
    21541 ttcagacaac cgaaaaaaga agtaaccaaa cgctttgtac aacaagacat cgaaccagaa
    21601 gctgattcga cagaactatt agctgaactc atcaaagaaa atccaagcgg ccgtttggca
    21661 actttgacct ttagcgaaag aaatgccaac gagccagtca tttcccaagc gattcgacgc
    21721 tataacatcg atgtcaacgt tgtgtatgga aagatcaaac aagtgaaaga aggttccttt
    21781 ggttcattga ctgtcttgat gactggtgaa accaaagaat tagatcaagc agaagtgttc
    21841 atcaaagaac aaggagtagg aattgaggtg atccatcgtg gataaaagct tttcagaaac
    21901 atatctagat tttaaacaaa tcaacccgga aacgatgaat caggctatcc ttgatacact
    21961 atatatgaca ttaatctcaa tggtcttagt gacgatcatc ggattagttt tgggattgat
    22021 cctttactct attggacgca aaaacaaacc gtatgctcga atcatttatg gaatcgtatc
    22081 gatcatcagt aatattttcc ggtcaacgcc atttatgatc ctgatggttt tgatcattcc
    22141 atttactaaa gcaatcgtag gcactatgct aggtgcgcaa gcagccattc cagctcttgt
    22201 tttatccgca gcaccatttt atgcccgtct agtagagatt gcttttcgtg aagtcagccc
    22261 tggtgtactg gaagcagcag atgcaatggg agcaagttac tgggaaatca tctggaaggt
    22321 cttgatacct gaaagtattc ccgcattgat ttctggatta acagttacaa cgatctcgat
    22381 gatcggattt actgcgatgg ccggcgcgat cggcgcaggt ggacttggag gtcttgcttg
    22441 gcaggaaggc taccagcgtg gtaatctaac agtgacattc gttgcgactt taattatttt
    22501 actaatcgta tttatcgttc aaggaatcgg tgatttctta actaagaaaa cagacaaaag
    22561 ataataggat atagggggat gtaggatatg aaaaagaaaa tttttggatt tgcagcagcg
    22621 cttttgctga ctgtaggatt agcagcatgc ggtaacaaca gcgacagcaa agattcagca
    22681 aataaagcag acgacacgac attgaaagtc ggcgcttcac cgacaccgca cgcagagatt
    22741 ttggaacatg tgaaaccatt attgaaagat gaaggaatcg atcttgaaat cgtcaaattc
    22801 gatgactatg tattaccgaa ccaatctttg gaagaaggcg acattgatgc caattacttc
    22861 cagcatattc cttatttgaa caaagaaatc aaagaaaaag gatatgactt tgtcaatgcc
    22921 ggtgctgtcc acatcgaacc aatgggcttg tactctaaaa aagtcaaaga tgtttccgaa
    22981 ttaaaagatg gtgcaacaat cattacttca aattctgaat cagactgggg acgcatcatc
    23041 acaattctac aagatgctgg cttagtcaaa gtcaaagatg gagtagatct ggaaacagca
    23101 acattcgatg atattgaaga aaaccctaag aacttgaagt tcaaccatac gatcgaccca
    23161 tcacttttag cttctacgta ccaaaatgat gaaggtgacc tagttgcaat caacgcaaac
    23221 ttcgcttacg gcgcagggtt gaatccatta gaagatgccg tattgcttga aaaggacaac
    23281 tctccttacg tgaatattat tgcgactcgt aaaggtgatg aagacagtga caaaatcaaa
    23341 aaactgattg aagtgcttca ccgcaaagaa gtccaagaat ggatcacaga caaatggggc
    23401 ggctctgtta aaccagtaaa ggctgatgca aaataaggta tggaacaaaa aagatgagaa
    23461 ataagtctgt aatggcttat ttctcatctt ttttatacct attctaattt agaaatcaaa
    23521 attgtttaat aataaataaa agatatttga ttttagtaag gaaggtcatt gcgcttcatt
    23581 ctcaattagt ctaaaatgag tgagaacgaa agagaatttg ggggtataat gcatgtctgt
    23641 cttagaaata aaaaatctac atgtgtcgat cgaggaaaaa gaaatcctta aaggcgtaaa
    23701 cctaacaatg aaaaccggag aaatccatgc catcatggga cctaacggca caggaaaatc
    23761 taccttatct gccgctatca tgggcaatcc aaactatgaa gtaacagaag gcgaaatttt
    23821 gtttgacggg aaaaatgttt tagacttgga agtagatgaa cgtgcgcgac ttggcttatt
    23881 tttagctatg caatatccaa gtgagatacc aggtatcacg aatgcggaat tcatgcgtgc
    23941 agcgatcaat gcgaaacgtg atgaaaacga caaaatttct gttatggcat ttttgaaaaa
    24001 gttagatgaa aaaatggctt tactaaatat gccagaagaa atggctgaac gttatctgaa
    24061 cgaaggtttt tctggcggag aaaagaaacg gaatgagatc ctgcaattgt tgatgttaga
    24121 acctacattt gcaattttag acgaaatcga ttcaggtctg gatatcgatg ctttgaaagt
    24181 cgttgctaaa ggagtaaatg aaatgcgcgg agacaatttc ggtgcattga tcatcaccca
    24241 ctatcaacga ttattgaact atatcacacc tgatgtcgtt cacatcatga tggaaggccg
    24301 agtagtcatg acagggaatg ctgacctagc taaacgtctg gaagcagaag gctacgctgg
    24361 tatcagtaaa gaattaggaa tcgactataa agaagaagaa gcgtaagggg gagcaagaat
    24421 gaaagaaaaa aactggctcg attatcttga cgcggtaaat gatttttctt taagtaaagg
    24481 cgaacctgat tggatgagaa ccttccgtca agacgcacta gcgaaagcag atgaactgcc
    24541 attgccgcat atcgatcggg tgaaattcca tcgctggtcg ttatttgatg tgaaagaaac
    24601 acaaaccatc agtgaaacag gcacgattcc ggcatttgat gcaatgaaag acaatcctgt
    24661 gcttgtccaa caaggttcat ggacgatttt tgaacaactg cctgtggaat tagcagaaaa
    24721 aggagtcatt tttaccgact tgttcactgc aatgatcgaa tatccagaac tagtccaaga
    24781 atattatatg aaaaaagcag taaacatgaa cgaagaccaa ttgactgcac tccatgtagc
    24841 ttttatgaac agcggtattt tcctgtatgt gcctaaaaat gtcgtgatcg atgaaccact
    24901 cgagtcctta ttcattcaag atggcgcatc cgatgagcac ttcttcaagc atgtgttgat
    24961 cgtggcagat gaacatagtg aattttctta tctggaacgt ttccagacaa ccaaagaaca
    25021 agtagcaaaa tcatcaggta atatcatcgt ggaagtcatc gctaaagctg gttcaaaaat
    25081 caaatattca gccgtagatc aattaggaga aaatattact tcttatatga atcgccgtgg
    25141 gcacatttta cgagatgctt ctgtcgactg ggcaattggt gtcatgaatg acggtcatgt
    25201 gatcgctgac tttgactcag atttagcagg agaaggtgca cacgcagaag tcaaaatcgt
    25261 ggcaatcagc tcgggcagac aaatccaagg aatcgataca cgagtaacca ataaagcccc
    25321 tcatacgatc gggcatattt tgcaacacgg agtgatccga gaaaaaggaa cattgacctt
    25381 taatggtatc ggccacatct tgaaaggggc aaaaggtgca gatgcccaac aagaaagtcg
    25441 tgtcttgatg ctttctgata aagcacgtgg tgatgcgaat cctattcttt tgatcgatga
    25501 aaacgaagta actgctggac atgctgccag tgtaggacga gttgatccag aagaaatgta
    25561 ctatctgatg agtagaggat tgcataaaga agaagcagag cgcttagtga ttcgaggatt
    25621 cctcggttct gtcttaacag caatccctgt agaacaagtt cggaaagaat tagtcgaagt
    25681 aatcgaaggg aagttgaatg gatgatcgat gctaaaaagc tacgcaatga ttttcctatc
    25741 ttatttcaag acgtcaatga tgaaccttta gtttatctgg ataatgcagc gacgacccaa
    25801 aagccaaaac aagtactcca ggcattagcg tactattacg aacacgataa cgccaatgtt
    25861 catcgcggtg tccatacgtt agcagaacgc gcgacccatg attatgaaca agcaagagaa
    25921 aaagtacggg cgttcatcca tgcaaaagaa acagcagaag tgctgtttac tcgtggtacg
    25981 acgacaagct tgaattgggt agccagaacc ttcggcgacc gctatatcaa agcaggagac
    26041 gagatagtgc tctcttacat ggaacatcat tctaatgtta tcccgtggca gcagttggca
    26101 aaacgaacag gtgctgtctt gaaatatatc gaactaacag aagaaggctt cctggatatg
    26161 gaggatgcag cgaaaaagat tacggacaaa accaaaatcg tctccattgc tcatgtatcc
    26221 aatgtcttag gtgtcttcaa tcctgttcaa gaattagctg aacttgtcca tgaaaacggc
    26281 ggggtcatcg tagtagatgg tgctcaatct gccccgcaca tggccattga tgtccaagca
    26341 ctagactgtg atttctttgc tttcagcgga cacaaaatgt gcggaccaac aggtattggt
    26401 gttttatatg gtaaacgaaa atggttagaa gaaatggaac ctgttgaatt tggtggcgaa
    26461 atgatcgatt ttgtcgaatt atacgacagt acatggaaag aactaccttg gaaattcgaa
    26521 gcagggacac caaatatcgc cggcgctatc gcattaggcc atgcaatcga ttatttgcaa
    26581 aaactcggta tggacaatat ccatcgatat gaagaagaac tagccgctta tgtgttgcca
    26641 aaattacaag caattgatgg attgactgtt tatggtccac aagacccgaa ttttcggaca
    26701 ggagtcgttg cattcaattt ggatgggtta catccccatg atgtcgcgac agctttagac
    26761 atggaagggg tagccgtcag agcaggtcat cattgtgcgc aaccgctatt gaagtacctt
    26821 gaagtatcag cgactgctcg cgcgagcttt tatttctaca acacaacgca agacgccgac
    26881 cgtttagttg aagcaatcat ggcaacaaag gagtttttcc aacatgggac tatctaaatt
    26941 agacaattta taccgtcaag tgatcttgga tcattcttcc catccgcatc atcgtggaag
    27001 cttgactgac tcgacaagca agatcgaatt gaacaacccc acatgtggag atgtgatcga
    27061 attgcaagtc gatgtaaaag acggaatcat caaaaatatc gcctttgacg gcagtggctg
    27121 ctccatcagt acggcaagtg cctcgatgat gacagacgcc gtcatcggaa aatcaataga
    27181 agaagcagaa catttaactg tagactttct acagcttgtc caaggaaaag aagtcaaaga
    27241 tcccgaccaa ttaggcgatg ctgctatgct tggtggtgta gctaaatttc ccgctcggat
    27301 caaatgtgcg acacttgcct ggaaagcact aggaaaagca atggaaaacg atggtaccgc
    27361 agctttccat cattaccaca aggaagagga gtgagaacat gggcgtacca gaattagaag
    27421 aatataaatt cgggtttcac gatgatgtcg aaccggtttt cagtacagga gaaggcttga
    27481 cagaagaagt gatccgtgag atgtcaagaa tcaaaggcga accagaatgg atgcttgaat
    27541 tccgtctgaa atctctagaa acatttaaca aaatgccaat gcaaacatgg ggccccgacc
    27601 tttcagacat cgatttcgat gccatcaaat attaccaaaa accaagtgac aaacccgctc
    27661 gtgactggga agacgtacca gaaaaaatca aagatacatt tgaaaagatt ggtataccag
    27721 aagctgaacg agcttatcta gccggtgctt ccgcacaata tgaatcagaa gtcgtgtacc
    27781 ataatatgaa agatgaattc gaaaagttag gaatcgtctt tactgacact gactctgctt
    27841 tgaaagaata ccctgaatta ttcaaagagt acttttcaaa acttgtaccg ccaaccgaca
    27901 acaaattagc agcgctgaat tcagccgtat ggtcaggtgg gacctttatt tacgtgccaa
    27961 aaggcgtcaa agtcgatgtt ccattacaaa cgtacttccg gatcaatgcg gaaaacacgg
    28021 ggcaattcga acgtacgctg atcattgttg acgaaggagc aagcatccac tatgtcgaag
    28081 gttgtacggc acctacttat tcaagtaaca gcctgcatgc cgccatcgta gaaatcttta
    28141 caagaaaaga tgcgtattgc cgttacacaa cgatccaaaa ctggtcagac aatgtgtaca
    28201 acttagtaac caaacgagca aaagcatacg aaggcgcaac cgttgaatgg atcgacggaa
    28261 acctaggtgc aaaaacaacg atgaaatacc caagtgttta cttagatgga aaaggtgcac
    28321 gaggcaccat gctctccatc gcctttgcag gagcaaacca aatccaagat acgggtgcaa
    28381 aaatgatcca caatgcacca aatacatcta gctctatcgt ctctaaatca atttcaaaag
    28441 acggcggaga agtaaactat cgaggacaag taacctttgc caaaaacagt gcgggttcga
    28501 tctctcatat cgaatgtgac acgatcatca tggacgaatg gtcgaaatcc gatacgatcc
    28561 cattcaatga gatccacaac agccaagtct ctttagaaca cgaagccaaa gtctcaaaaa
    28621 tctcagaaga acaactctac tatctgatga gccgaggcct gaccgaatct gaagcaactg
    28681 aaatgatcgt catgggcttt gttgaaccat tcaccaaaga acttccaatg gaatatgcag
    28741 tcgaactcaa tcgattgatc agttatgaga tggaaggcag tgttgggtaa acctgttata
    28801 ggagctagaa cccttgatat ataagggttc tagctttttt gttttgcctg tatactagaa
    28861 atgaccagat tttgaccagc tcttttactg atgagccatc atatcagcaa agtcttgacc
    28921 tgttttctta ggtgcattat ttgtaatatg tgcatagatg tctaatgtca ctttactatt
    28981 ttcatgccct aatcgttgtt gaactacctt gacagattct cctgcttcta ataataaact
    29041 agcatgtgta tgtctaaacc catgaggtgt aatacgaggc aagttatatt ttttcaaaat
    29101 atatcttaac caatcattaa cagtgtttgg aatgtataga gtgtttttaa ctgttgtaaa
    29161 aacatgttgc tctttagagc tagtattgta tccaagtttt aaatactctt ctttttgctg
    29221 taatttccag ttgttgagga tagaaagagt ctcagtatct aaagagataa ctcgtctaga
    29281 agctcttgtt ttcggagatt gaattataac tttaccaaat tcatctacag caagagtttt
    29341 attgacatgt agttctttat taaacgtgtc tatgtctttc cactgtaaag cgagtacttc
    29401 actctttctc atacctgtgt atgccaataa tctgaagaag gcgtattgtt tcatgttacc
    29461 aaaatcttta aaagcatcca gtaaattatt aagctcctct tttgagtaaa atttacgatt
    29521 tttctcatac tctttttttc gtggaagaag tgttttttcc ataggattat tgtcaatata
    29581 ttgcattgca accccgtagc gtaaaatttg tgcagtctgt tttctcatat aatcgtaact
    29641 ttcatattta ctgtgccatt catctaccac tttttgacaa tatgggacag agatattcga
    29701 tagtttaagc ttaccaaatg ctggaagtat ttgattattt gcgtatctta ctgagattgc
    29761 gattgttgaa gctttaacac tggtacgctg atgagttatc cattcattat acaggtcttc
    29821 aaatgtcata tcaggtgctt tctttttaac accgtataat atttgatgtc tgtactcgtc
    29881 ataatctttt tgagcttctt gtttgctact aaaacctctt ctcgtagtat ttattttttc
    29941 accagttcgt ggatcggtgg caatgtgtcc tttatacatc cacgccactt gaccgttttt
    30001 ctttttgtac tttgatattt ttgccattaa atcatctcct cttagtttac aaggagtact
    30061 taccactaga tggtatcacc tcccttcatt tttttcttag tgtatttttt cccaattgat
    30121 gaaatcgttt gttctagctt gtttatatta tcagtaattt ctctcacagc ttcttcggga
    30181 taagacatgt ctttcaattg aataggtagc cattcatcta ccattctttc tagtagttct
    30241 tctatactgc tagtgaggaa aagaatgtta ttgtcaaatt cttctacaat agcattgatg
    30301 aataattctt ctatttttaa gatattgaaa agactaattt tttcattttt aactttatta
    30361 caagtcttgt tagcaataaa ttcttgcatc tctggttcta atttggaata tctatccagc
    30421 catgtatcta aattagagtg tactggtggg aactgttgac gaagatgctg gaacaggttt
    30481 ttatctggaa atgttatacc atctagtaca atgctatctc tataaagaat aattcccaaa
    30541 atatattctt ctacattgcc acaaactaac tcctctatac tcatatttcc caaatcagca
    30601 attcgggata atcgctctgg tattggtaaa taggttccct tctcccattc gtagatagtt
    30661 tttttgtcaa ttaattgacc gttgttaatc ataagaccaa atgctggttg tgttaatttt
    30721 ttactctttc ttatagcttt tatttttgtt ccgagtatct tttttcgttc taaataattt
    30781 attggtggac gttttatcat attgaccaca cctcctaaaa ttagtatacc tcaaaaaagg
    30841 ggtgttgcaa ataaatttta caaagctgtt gacacccctt ttttatcgag gtattatgtg
    30901 tatgtcgatg aactggtaat cattttttgt ttgattaatc ggttaataac aaaatacttt
    30961 tattttgagc aaaaaatacg aagataattc ttagaaaatt tgttcaagca atcacgaaag
    31021 gagggggaaa tatgtctcta caaatcattg atagcattgt ttctatacaa gttgaacaac
    31081 aagtgaaaga gataattaca gtgctaaagc ctatgctaga agacattatt tctggagtca
    31141 ctctagagta tatggactat cagcaggcaa gtgaatatct aggtgtatca gtaggaacga
    31201 ttagaaaata tgtttctcaa tacgggttac ctgttataaa aattgatacg gtcattagat
    31261 ttaaaaaatc tgatattgat agtttcatgg aacggtataa aggttgagta aacaaataag
    31321 actaaaacat atagtaataa agagtttgtt tttattatct gtatgtttta gtctttaaca
    31381 gaattttata aggaggaaac acaaatggaa acatcaatga ttaatattcc aatttatgta
    31441 gatcaagaaa aattattaga gaagccagtt acaaaaaaag aaaaaactga tgtaagagat
    31501 aggtgtttag ataactatag agaagttaca agagatgagt ttgtagagat actaaattca
    31561 tcacaatcat ttattccaag caagacaaag aataaaggca atgacaagga gagttttgtg
    31621 gaaaccagag taattattct ggatgtcgat aacactgtta aagatgagaa ggggaaggta
    31681 gtagatttga gtaaagatga tagtagatac ttaagcgtag aaaaggtttt atctatcaat
    31741 ttagttcata attctgcgtt tgcgatacaa aaaagtattc ggtactctga aaatctagaa
    31801 aaatacaaga ttgtctttct attaaaagaa gcaattactg actatggtaa aatggttgct
    31861 gtttacgaat acttgaaaaa gaacatcccc gggtgtgatg acaatgtaaa tgcttcgaat
    31921 agaatgtttt ttggaggata taagtcagat gcagtgataa taattaatga agacaacatg
    31981 ttagatatta ctgagcttcc gattgatttt accatgacat gttctaaaaa ttgtcaaagt
    32041 gattcaggga gagagtatga actagtcaat gaaaccgatt ttgttaagtt gataaaaaat
    32101 gacgacaaag aagaaatgaa acagtggttt aaagactgcc tgtttgattc ctccgacatg
    32161 tcttttaatg agatttatga aagattgtta accattgata tgaataaatt acttaagtca
    32221 agtaaaaact taagatgtct gtttcacaac gatcataatc cctctgctag tatttttacc
    32281 agtaaaactg ggcattccat tttttattgt cattccagta attgtgatat cagtgaaaat
    32341 tttattggag ttgtgatgct aattttaaag aaagacagtc gggtagaaac atttaattgg
    32401 ctattagaga acctagactt gtatcctaca cattttcgaa aattgaaaga ggaatcaaaa
    32461 gtactttttg acactcttga atcaatgaaa gacaaacttt tcaggacaat aaggaattat
    32521 ctagaggaaa tgcgacgtgt ctatgatgtc ttgctttctg atgttaattt ttttgatgat
    32581 agtaaggaaa gtgtgacttg tattttgtcg ggggaacaat tagctaaaaa aatgagttct
    32641 tactatggta aaacttatga catagataaa tgtaataagt tactatcact tatgactttt
    32701 ataggattga taaaaaaatt agatgatgaa gatgttcctt tgaagatgct cgaataccta
    32761 aataggttaa aggaagagaa agcgttagga aactttaatc ataagagaag caatgtctat
    32821 cgattagacg tttttaatgc aacttctgtg attcaaaaat tgaatgaaga tatccttcca
    32881 cttctggaaa aaattcattt tacatatcaa tatttttctt atgaatgggt taagatatgt
    32941 tttaacgaaa aagaggctaa aagagtattt cctcaaaatt cccgatcaga actcagcaag
    33001 gaaaaacaat tgattttaga tgaagcacat gaagttttac agggcttatc gtggcaaggg
    33061 acatatctaa tgacagagaa agaacttatt aacactgttt gtgaacgcct acgtaatgtt
    33121 ggggataaca gcgtgaaaaa tgctttaata aaaaacagag gctactttgt taaacaagga
    33181 ttcactcttg aacgagcttc taaggatatt aagaaactat acaatgtgaa agcaagtagt
    33241 agttttttaa tatatttagc gaaaggggca gggatagatg aaacaataaa agaattgata
    33301 ttatcaggag ctgggatagt tcctctgtat caatttacaa gtgtaacaag agatgatgga
    33361 cggagagtaa ggaagagaaa agacccaatt gtattaaact agacaggtta tccatagctc
    33421 ttaatcttat ttcatctcaa tacacccacc gttaaagttc ggtgggttat ttatttttca
    33481 atctttcaat agctgattgc gttcattttc ttgccttttt aatagcttca tatagtcaat
    33541 tgttcgaact gcatcttcag tgctaagaga acgatagaga gccaaaaact cttcctcata
    33601 atctacgaca acattgtcta gaaaatggct tacaggggtt cctagggctc tagaaagagc
    33661 tgttaagcta tcgaggttag gctctgttac agaattttcc catcttgcta ctgcctgttg
    33721 tgtagagaag attttttcag ctaattcttt ttgtgtccac ccgagagctt ttcgcttttc
    33781 tctaatgacc tttgcatatt gtttctgttt cattaataca cccccatctc agtatagtac
    33841 aacaagttgt tgtatttgta atggtatttg aagtttttta tattttatga gttgaaatac
    33901 atcatattgt tgtatataat aaaacaggag gggagaatca tgaaaaaagt aagtaatctc
    33961 agccgataca gaaaagaaaa aggactttct caaacggagt tagcaaaaaa aatgaatgtt
    34021 acacagcaat gtgtaagttc gtggcaaaca gggagaacga ttcctaaacc ctatcaaatg
    34081 aagatgcttt ctgagatatt aagtgtacca atgaatgagc tgttttctga tgtatttaat
    34141 aaagtaaaca gttaactaat aagcaattga atgattttag gaggagaaga catgtattgt
    34201 agaaaatgtg gcaaagaaat aacagatgaa agtaagtatt gccagttttg tggagcacta
    34261 caagaaacag atgagacaac cggaacagac acagtaaaga aaagtactga gcagacaatt
    34321 aatgaaatga aggaacaaat cgtacatgga caagagacag ggaagctgta tttgattatt
    34381 ggttggattt ctatggtagt atcgttagca tttattcctg ttgtatttgg tgctgttgct
    34441 gttattatgg gctacttata tcgagaaaaa gatgagaaaa tgggtacgat tttgatgata
    34501 gcaggggtag ctggagcaat ctttggtgta cttttaggga tgtcaatgta ctgataagag
    34561 ggataagagc aaattatttt ggagatagga gataaaaagt tgaataaagt aaatatgaac
    34621 aagaagtttg tcattatagg tattgttgtc ttggctatta taggtattat tttttcaaaa
    34681 atgacaggta catctggatt agagaagtca tcaaaaccat tggtaacaca gattttacaa
    34741 gaacagtaca acttaaatcg atcatgtgat gatgtgacga ttactcatga aaatggagat
    34801 aacacatacc gggctaaagc tatattagac aatggaagtg ctatcaacat aaatattgag
    34861 tactatccaa agaaagaccg tatatatgtg gagataccat atgctgaggt gttaatgtta
    34921 aattgattta aatgtattat gcataccgtc caaacaggaa ctcccaaagg tgtaaattca
    34981 ctagggagtt tttatagttt cctataaatt aaatactaat acaggtgaca aaaaaatatt
    35041 acaatgattg tcgggaattt ttatgttatt cactgttata acaatataaa agaaatgaaa
    35101 aagaaaaaat tattatggat atgtttggta tttttattga
//
LOCUS       DARBDV010000016        34892 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000016 DARBDV010000000
VERSION     DARBDV010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.016"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(59..187)
                     /locus_tag="SCM56_000804"
     CDS             complement(59..187)
                     /locus_tag="SCM56_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEG1109626.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="SCM56_000805"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="SCM56_000805"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEG1109627.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="SCM56_000806"
     CDS             complement(1674..2729)
                     /locus_tag="SCM56_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEG1109628.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="SCM56_000807"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="SCM56_000807"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEG1109629.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="SCM56_000808"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="SCM56_000808"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEG1109630.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="SCM56_000809"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="SCM56_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEG1109631.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="SCM56_000810"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="SCM56_000810"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEG1109632.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="SCM56_000811"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="SCM56_000811"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEG1109633.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="SCM56_000812"
     CDS             10491..12050
                     /locus_tag="SCM56_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HEG1109634.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="SCM56_000813"
     CDS             complement(12161..14092)
                     /locus_tag="SCM56_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1109635.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="SCM56_000814"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="SCM56_000814"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEG1109636.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="SCM56_000815"
     CDS             complement(15021..15773)
                     /locus_tag="SCM56_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEG1109637.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15932..16141)
                     /locus_tag="SCM56_000816"
     CDS             complement(15932..16141)
                     /locus_tag="SCM56_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109638.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16152..17480)
                     /locus_tag="SCM56_000817"
     CDS             complement(16152..17480)
                     /locus_tag="SCM56_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109639.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            17616..17771
                     /locus_tag="SCM56_000818"
     CDS             17616..17771
                     /locus_tag="SCM56_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109640.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(17845..18432)
                     /locus_tag="SCM56_000819"
     CDS             complement(17845..18432)
                     /locus_tag="SCM56_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEG1109641.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="SCM56_000820"
     CDS             complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="SCM56_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEG1109642.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20012..21301)
                     /locus_tag="SCM56_000821"
     CDS             complement(20012..21301)
                     /locus_tag="SCM56_000821"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEG1109643.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21455..22387)
                     /locus_tag="SCM56_000822"
     CDS             complement(21455..22387)
                     /locus_tag="SCM56_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109644.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22544..23203)
                     /locus_tag="SCM56_000823"
     CDS             complement(22544..23203)
                     /locus_tag="SCM56_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEG1109645.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="SCM56_000824"
     CDS             complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="SCM56_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEG1109646.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24244..24534)
                     /locus_tag="SCM56_000825"
     CDS             complement(24244..24534)
                     /locus_tag="SCM56_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109647.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24943..26748
                     /gene="glmS"
                     /locus_tag="SCM56_000826"
     CDS             24943..26748
                     /gene="glmS"
                     /locus_tag="SCM56_000826"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEG1109648.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26820..28175)
                     /locus_tag="SCM56_000827"
     CDS             complement(26820..28175)
                     /locus_tag="SCM56_000827"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEG1109649.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28199..29368)
                     /locus_tag="SCM56_000828"
     CDS             complement(28199..29368)
                     /locus_tag="SCM56_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109650.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29365..30252)
                     /locus_tag="SCM56_000829"
     CDS             complement(29365..30252)
                     /locus_tag="SCM56_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEG1109651.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(30441..34133)
                     /gene="nifJ"
                     /locus_tag="SCM56_000830"
     CDS             complement(30441..34133)
                     /gene="nifJ"
                     /locus_tag="SCM56_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HEG1109652.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            34332..34853
                     /locus_tag="SCM56_000831"
     CDS             34332..34853
                     /locus_tag="SCM56_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEG1109653.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT         9411 a         7572 c         6288 g        11621 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcctgc ttttttgaag taagcgtcat aataacccgc gcttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tcagagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 aattggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcctcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgtttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttccgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgatagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcgattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg cgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ctccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggaa attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat gtcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt taccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccggtcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatacgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaatgtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtgggg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagaggc tgggaaaacc cagcctcttc ttttttattt ctatgcttct gctactgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgccg tgaggagcca
    12301 gtaatttgat tccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcaactc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcaaagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccagcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagtaat tttttcccat acggaatgat ttcttctaca gattggaagg ttgtgttgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaac ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagctggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cctaagccat tgatctcagt ttcagtgtct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg gatccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattcaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctgcgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctacta gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcag aaagactggt
    15901 tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt
    15961 ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa taaagtccca
    16021 tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa
    16081 agagacagac ggataataat gatgccggaa ccattattcg ataatagagt tgtttatcca
    16141 ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa
    16201 aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact tcgccgcttt
    16261 ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt taacggtaat
    16321 tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc ttgatatttt
    16381 tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc ttcgattcca
    16441 ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgtga tcgattctcg
    16501 tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct ttctttataa
    16561 tctccttgtt ggatatcata actttttgtt acaaaaaatg aagcattctt tttaggaaca
    16621 ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt gtaaatgtat
    16681 tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg atcaaaccca
    16741 agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca gttaaagggg
    16801 tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt ttctacttgt
    16861 tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact ataatacatt
    16921 tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg aaattggttg
    16981 caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc gatttcatcc
    17041 tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat aaactcgcta
    17101 taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat gaagtctcga
    17161 atcaaaaatt gcttttcttt gaaaacagag atagacaata cattttttat caataattga
    17221 ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa ccaactttcg
    17281 tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata cggatccgct
    17341 tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa
    17401 tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgagctggat agtcatcaat
    17461 aatttatctt cgccaatcat tttctcacct ctttcaaatc tttttataat ttaattataa
    17521 ccaatgataa agaaaaacta aaactttatg tcttagaaac caatttggac gattacatga
    17581 tagactaagt aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt gaacgtacag
    17641 ataatttaga taaacttttc gaatcatttg ctgtcgatcc ggaaaaaaca gttgcaccaa
    17701 aagaagagat cgatcgattt ctaaaaccac agccacaaaa tgaaaagaaa aaagacaaga
    17761 aacaaaagta agttaagctc aaaaaaagac ttaccagcca aacttttatg aaagagtttg
    17821 actggtaagt cttttttatt ctttttatga ttcaattgct tgttcgattg ctgcccacgc
    17881 ttcttctttt ccctgttttg taacggaaga aaagagaata aacgtgtcat tgcgatcaaa
    17941 atccaacgct tttttgattt cgctctcatg cttgttccat ttaccacgag ggatcttatc
    18001 tgccttagtg gcaacaacaa ttacagggat atcataatac ttcaaaaatt gatacatctg
    18061 gatatcttct tgagaaggct tatgacgcaa atctactaaa gaaacgaccg cacgtagctg
    18121 gtctctagtc gttaaatatg tctcgatcat ctttccccat tttgctcgct cagttttgga
    18181 tacttttgca tacccatatc ccggaacatc gacaaaatgt aatgcgttct cgatcaaata
    18241 aaaattcaat gtctgggtct ttcctggctt accggaagta cgggctaaat tctttcgatc
    18301 aatcaatgtg ttgataaaag aagatttccc tacatttgat cgtcctgcca gagctatttc
    18361 tggcaattgt gtatctggat attgttttgg tgccactgca cttatgataa tttcagcttg
    18421 atggactttc atggtctctc tccttaacaa ggtgatgaat aaagcaagcg gctgtcgcaa
    18481 aagccaaaat gacttttacg gcagcctctt ttcatcaacc cgcttttttc tctgaatctt
    18541 tatagattac tgttggctca ccagacaatt cagctgcttc ttttgtgata atcacttttt
    18601 cgatgttttc atttgaaggg atatcaaaca tcacatccat catgatttct tcaatgatgg
    18661 aacgaagtcc tcgagcaccg gtattgcgtt cgattgcttt tttcgcaatg gctctcaacg
    18721 cttcaggttc gaattcaagt tcggtattat ctagagaaag aagtttttga tattgtttga
    18781 ctagcgcatt tttcggctca gtcaagatcc gtaccagatc atctgttgtc aatttctcta
    18841 aagctgccat aacaggtaat cgtccgataa actcagggat caatccgaat tttagtaaat
    18901 cttcaggaat gatatgctgc atgacacttt cgtcttcaga aagtttttgg ttttgtgtac
    18961 caaatccaat cgtcttttcg cccatacgat tcttcacaat cgtttcgatg ccatcaaatg
    19021 caccacctac gatgaacaat atgttcgtcg tatcaatctg aataaattct tgatgtggat
    19081 gtttacgtcc gccctgaggt ggcacacttg caacagttcc ttctagaatt ttcagcaagg
    19141 cttgttggac accttcgccg gaaacgtcac gagtaataga tacattttcg cttttacgcg
    19201 caattttatc gatctcatca atatagatga tccctttttc tgcaagttcc acattgtaat
    19261 cagcagattg aagcaattta agcaaaatgt tttctacatc ttcccccaca taaccagctt
    19321 ctgtcaaact tgtcgcatct gcgattgcaa aaggtacatt caatgttttt gctaatgttt
    19381 gtgctaaaaa tgttttacca gaccctgtag gtccgatcaa gcagatattg cttttttgca
    19441 gttcaacatc atctgtgttt tcttgtgctt gctgattgac acgtttatag tgattgtata
    19501 cggcaacagc taacgagcgt tttgcgcgat cttgtccaat cacgtagtta ttaaatactt
    19561 caagaatttc tagtggttta gggacttcag tgaattcgcg tacagcttct tcataaaatt
    19621 cttcatcaat gatttcttta cataggtcga tacattcatt acagatgtag acccctggac
    19681 cagctacgat ctttttcact tcttcttggg ttttcccgca aaaagaacag cgtacggtct
    19741 cattgctact tggattgtca tacatggtct tcacccctta acttgcaaaa agtatgcaag
    19801 aaactatcaa agactttaca gtcttttact atcataacat agtttagtaa aaagaaaaag
    19861 tgtttaacaa aggatcaact gacctatacc tttgcatttt ttactcttaa caaaaaataa
    19921 ggaactgtga caaaatcgtg tcacagtccc ttatttcgaa taaacaattt tctagaagta
    19981 gcttcttcag aaatgattta ttacttgatt tctattcttt cgcttctgtc gctgtacctg
    20041 tgatcaattc gattgctttt ttcattgtca catcatgttc cagcatatct tctgtcaaca
    20101 cgcgtttgat ttgttcgatt ggcatgttgt atgtttctga taattcttgg atttctgcat
    20161 tgatttcttc ttctgttgct tgaagatttt cagcagctgc aattgcttcg atcacaagat
    20221 ttgttcttgt gcgcatttct gcttcacctt cgaattgttt atgcaagtct tcttctgtag
    20281 aaccagttaa ttgatagtac atttcaggag agataccttg acgttgcatg ttgttcaaga
    20341 attcgtccat tgaacgatgt acttcatcat gaaccatcac gtgtggcaat tcaacgattt
    20401 ctgcgttttc aacagccata cggatcgctg cttcatcttt tgcatcgtcc gctgcttttt
    20461 cttttgtttc tactaattct ttgcgatatt tttctttcaa ttcgtctaat gttgatactt
    20521 cgtcatcaac atcttttgcg aattcgtcat ctaattcagg cacttctttt gcttttactt
    20581 catggattgt cactttgaag actgcttctt ttccagcaag atcttctgct tgataatctt
    20641 ctgggaaagt tacatttaca tctagacttt cacctgattt atgaccaact aattgatctt
    20701 caaaacctgg aatgaatgag cctgaaccta attcaagtga gtaattttcg ccttttccgc
    20761 catcaaacgc ttcgccatct acaaagcctt cgaagtcgat tactactgta tcgccatttt
    20821 cagctgcagc atcttctttg ataactaatt cagcttgtgc ttcttgttca cgtttgatac
    20881 gtgcatccac gtcagcatct gttacttcac ggtcttgttt ttcaacaact agatctttat
    20941 attgtcctaa ttttacttct ggtttcactg ttacttcagc agtgattacc cagtcttgac
    21001 ctttttccat gctttcaaca tcgatttttg gttgagaaac aggatcgatg ccagcttctt
    21061 tgacagcagc ttcataagct tctggtaata cagcattcaa tgcatcttca tataaagctt
    21121 cttcaccgta catacggttg aatacttgac gagaaacttt ccctttacgg aatccaggta
    21181 cgttcaagct ttttttcact ttattaaacg cagttgttaa tcctttttgg atttcgtctt
    21241 gcgcaatcgt aaattttaaa acaccatcat tcgtgccttt tttttcaaat gttgcagtca
    21301 tttaattccc tccgagttta gaaattcatt ttatacatca aaaacgtttc atgcatacct
    21361 ttaaatagta cactaaccct ttcaaattgt aaacatattg aatggataga atccttgttt
    21421 ttcataacat ttttatcttt ttcactgata aaatctaatg gaagaagatc atttgctgca
    21481 aaattttttc tctttttttt aaaaaatctt gtttttcatc ttctgtacct tcatacatcg
    21541 tttttcctgt acacgcctct acagtcagtt gaaaccagcg cttcgggtct tcgatgtatg
    21601 tatcatagac aggataaaca attgcagatt gcaacgtgaa ttcttctttt aacacggaac
    21661 taagcagcgc atcttcattc ccgtaacgat ctgaaaatac acataattgc tggtaacttg
    21721 cctgctgctg cggctttggc agatcttttg gtatgacttt gactaacttt tcttcaatcg
    21781 tcaaatagaa aacaggatgg cattcgttca attgcctaag tgtctccagt aattttgccc
    21841 ttgctaaaat atggacctct ggcgatacaa taagtttact cgctgtttgg ataaactcat
    21901 gataaggaag ctgttcgatc tcatcaatag acgcaagctg acgtgcaggt tccaacgaag
    21961 gaagttcctc cgatttattg tggagtgcct tgatcttctg cctctccatt tgtccggata
    22021 attcttctgc aaattggatt ttttcaatga cccgctgctc gaaagcttca taaaaatctg
    22081 tacttgctat caattttcta gcatataaaa aatcttgtgt actgattaac aagtcaaaat
    22141 aaaattcagc aaactcctca ttttgaagat actgtttttc atgcaagagt gcttgcttca
    22201 atgcttcttt tttctcgtcc aactcgtaaa gacaaaaaac aagcaggcta ttagcctcaa
    22261 agctttcttt caactcgtaa gctcgttgaa ataattcttt tgcctgctga tattgctggt
    22321 ttgctagtgc ttgttgtgcc actcgcatta aattctcata tttttctgga aaatcaatcg
    22381 gcttcacgca gtgtcctcct cttgctcata ttcagctcat gttcaatagt agcaaaacaa
    22441 aaaaagcatg acaattaaaa ataactgaag caccctctaa aagctagatt ttagatctga
    22501 cttttagaag gtgctttcaa ctgttactgc tttttattgc tcatcagctg ttgacttcct
    22561 ggttgaacgc tttgatcaga ttgatcgtaa caacacttgt attagtgaaa aaatcgccca
    22621 gatgttgctt tttctgatta aagactgctg gaagataccc taacatcagc aaaggaaatt
    22681 ttagaatgaa ccggcaagca ccttctctaa ctaaaacggt tgaccagcta agttcttctt
    22741 catcaaagct gatgactcga ataccaaaga tcattttccc aatcgtttgg ccatgattga
    22801 atttcgtcaa taaaataaaa tacgctagat aaataagtaa ggctgtgaat ccgtagagac
    22861 tcagataaga atctgaaaaa ttaagccaac ctagattttt ggcaatacca agtgttaaac
    22921 gcgtaatagc tccgatacac aaaagatcga ccaagaaggc aaacattcgg ataaaaaaac
    22981 ctgcatagaa atacttagga aaatcatttg cttttggcgg ttcatttctt gtgtccgtat
    23041 actttttcca tttacgttgt ttctttttga tttcttcggg cgtcaaaggc tctctttcaa
    23101 aagagctcag taccttttgg gaaaagttgg agtcaggtcc tacagcaggc tgctgttgct
    23161 tatctttttc ttgttcctta ttttcatttg aaagctctga catgcttact cacctccgta
    23221 ataatacatt gcttttggtg attccgctgt ccctagattc tcaagaacgg ataagatctg
    23281 actagtctca gaaggcttca atccctggaa ttctgctagt ttacttccta accatgtact
    23341 agcaaaccca gatggcgtgg aagaatattc tactagttcc gcatcttcaa gctgttgttc
    23401 ctttcgtata gcttttaaag catcttcagg aaatcccaat tcatcgacta aacctacttc
    23461 ttttgcttgg acaccatcat aaatccttcc atcagcaatt tttttgactt cttcttcaga
    23521 tttattccgt ccctcgctga cgattgctac aaaacgacta tatgcgctgt cgatatatgc
    23581 ttgaagcacc gcttcttctt cttttgtctc tggtcgcgtt cctgaaccca tatctttcaa
    23641 cgctccgctt ttaactgttg tatcttccac acccagtttt tctagaagtc ctgaataatt
    23701 caatcctgac atgatcacac caattgagcc ggtcactgtt tcttctgttg caaagatttt
    23761 atctgcttga gcagaaatat agtagccacc gctagctgcc atgtttttca tgctgacata
    23821 cattgggata tcatgttctt tacgaatcgt atccaatttc ttagcgattt ccgcactttc
    23881 gtagacaccg ccaccaggag aatttacttc taaaaagata cctttgactg ttggatcttc
    23941 ttcaatcgcc ttcagttgtt ccataaaatt tttatgattg taaccttcag aagcaaataa
    24001 acctgaagac cctcccgcag caatcgttcc atctactgat aattttacga ttttttgcgt
    24061 actgtctccc tcttccaaca catttggagc tagttcttca tccccatata accaactgtt
    24121 caatccctca atttgatcgt tttcctgagg cttagtcaaa ctagctgaaa ctaacgaaac
    24181 cacaaataat cctgcagcaa tcagaactgc tgcccaacgt cttttattca ttctttctcc
    24241 tttctaatac aagtttttta tgacgatcac agtttctgac tgctcagaag tgtcgaaccg
    24301 ctctgctttt tcttcccgcg catacccgga aaacgactct tttgttaatt catcttctac
    24361 cgtaaactca cgagcttgag caacttccaa ggcatcaggt gcgatttctt ggatgctatc
    24421 ccagccagta tctaaaaata ttttcgtatc atctgataca ccagctgatg cttcgaattt
    24481 ggacaattgt ttttttaaca ttcccagtgt catgatctca atttttgaat ccatttttac
    24541 tcctcctcta tccgattact ttctattgta ccgaactcct gaaaaagatg atagataaaa
    24601 acccattatt ttttgacata aaaaggacat ttgacaaaga atttgatata taccaattta
    24661 ttttttattc tttttttgtt gacaagaaca ttcgcaaaag gttatgattt taaatgtttc
    24721 cagataaaaa acaaaaagga ggatagttat accttttagg atagcgccag gtctgtgtaa
    24781 acagatgacg aggagagggt ttatcgaaag attcggcgga tggccctagg gactgcactc
    24841 tacagaaaat ccacaaaaaa tgtctgcaaa gataaaacaa aaggattttc ctgcagctgg
    24901 aagcattcat acgaattcat tatagaaagt aggatgtttt ttatgtgcgg aattgttgga
    24961 atgattggat taaaaaatgt aacaccagga ttgattgacg gtctagaaaa attagaatac
    25021 cgtggttatg attcagctgg tatctttgtc tcagatggca caactgatta tctggtcaaa
    25081 gcacaaggca gaattcaaaa tttgaaaaac aaaataaata ttgatactac aggaaatata
    25141 ggtattggtc atacacgatg ggcaactcac gggcaacctt ctgaagaaaa cgcccatcca
    25201 catacttccc aaagtggacg cttcgtgctc gttcacaacg gcgtaatcga aaactttgaa
    25261 gaacttaaaa ttgcttattt agcaaatgac cactttattg gtgagaccga tactgagatt
    25321 atcgctcatc taattgaaac tttcgcgaag gatactacaa cgcaagaggc gtttttaaaa
    25381 gctttgcggg tcatcaaagg ttcttacgct ttcgctttga ttgatcggac agcacctgat
    25441 gttatttacg tcgctaaaaa taaaagtcca ctattaatcg gactcggcga tggattcaat
    25501 gtgattgcca gcgatgcaat ggctatgctt gctcatacga aggaattcgt tgagattgaa
    25561 gatgaagaga tggttaccgt cacatcagaa aaagttatta ttcaaaattt tgcaggtgac
    25621 atcgtagaac gttcttcatt cgaagcacaa gtagatgctt cagatatcga gaaaggtacc
    25681 tatccttttt atatgctgaa agaaatcgac gaacagccta ttatcatgcg tcgtatcgct
    25741 caaaaatacg taacagaaga caatcgtgtc tctcttgatc aaaagctggt cgatacattg
    25801 tctgatagcg atcgaatcta tatcgttgct tgcggaacta gttaccatgc ggggttagca
    25861 ggtaaacaga ctttggaaaa attgacacag attcctgttg aggttcattt agccagtgaa
    25921 tttggctata acacacctct tctttcaaaa aaaccattct ttattttctt gagtcaaagt
    25981 ggcgaaacag cagacagccg ccaagtgctg gtaaaaatca accgtctcgg ctacccttca
    26041 cttacgatta caaatgttgc tggttctact ctttcgcggg aagcatcctt tacactgcta
    26101 ttgcatgctg gacccgaaat cgcagtagca tcaacgaaag cttatactgc acaaatagct
    26161 gtactaactt tattagccaa agctatcggt gataaaaaag aatttgaaac ttctcttgta
    26221 ttcgatatca ctcatgaatt gagtttagtt gctaatgcga tggaatcggt catcgcccaa
    26281 aaagattatt tagaagagtt ggcagaagta tatctcagcg atacaagaaa tgccttttac
    26341 attggacgtg gtgctgacta caacgtttct cttgaagctg ctttgaaatt gaaagaaatc
    26401 agttatatcc aggcagaagg ttttgccgct ggcgaattga aacatggaac gattgctttg
    26461 atcgaagaag gaacaccagt gatcggaatc atcagtgaag aagtgactgg tgcgcatact
    26521 cgaggaaacc tgaaagaagt cgaaagccgt ggcgctaaaa cactagttat tgtctcagag
    26581 ggattagaaa aggaaagtga ccagctggtg ctaccaactg tccaccctta tctaactact
    26641 ctaaccacag tcgtaccgac tcaattactt gcatattacg caacgctatt acggggattc
    26701 gacgttgaca aaccacgtaa tttagcgaaa tcagtgacag tagaataata aaagaacaag
    26761 gaccagtgtc agacatctga cactggtcct tgttctttta ttttttatga aaatatctct
    26821 taatccagtc cgatttcttc tttgactact gccgcgattt tatcgacata gtaattaact
    26881 ttctcatcag taggcgcttc tgccatcact cgaagtaatg gttctgtacc ggaaggacga
    26941 actaggattc ttccttctga tcccatttca gcttctgcct cttcaatcac ttttttgatt
    27001 gccggaacat ccatcgcacc gtttttatca cttacgcgga tattcactaa tttttgcgga
    27061 tagattgtta cctctgctgc aagctctgat aatttcttac ctgtctgttt catgatattc
    27121 agtaactgaa ttcctgaaag catcccatct ccagttgtgt taaaatctaa gaaaatcatg
    27181 tgcccagact gctcgccgcc aaagttataa tcgtttttgc gcatttcttc tacaacgtaa
    27241 cgatcaccaa cttgtgtaac gacatctttc aaaccgattc cttccaccgc tttgtggaat
    27301 cctaagttac tcatgacagt cgtaacgatc gtatcttttt tcaatcgatt tttttctgcc
    27361 aagtatttag cacagataaa catgatttta tcaccgtcga caatgtttcc aagttcatcc
    27421 accgcaataa tacgatcgcc atcgccatcg aaagctagtc cagcatctgc tcctttttca
    27481 acgaccatct cagctaaacg ttctggatgg gtagagccta cgccgtcatt gatatttaac
    27541 ccgtctggat ttgtgcccat tgtataaaaa tctgtttcaa gatcggcaaa caaacgatta
    27601 acacttgttg aagttgctcc gtttgctgca tcgacacaaa tggtcaaacc ggaaaggtca
    27661 ccgttgatcg tttgtacaag gaattgagaa tatttcaata agccttctgg aaattcttct
    27721 acagttccca atccttcagc tgatggacga ggcaattcat ccacttcagc atctaataat
    27781 gcttcaattt ctagctcttg gtcatctact agtttaaaac catctgcacc gaaaaatttg
    27841 attccattat cttgtgctgg attatgagaa gcagaaatca tgacccctgc gcttgctttt
    27901 tgcaaacgtg tcagataagc aacacctggt gtagagatca cacctagctg gaacacttcg
    27961 atacccaccg ataaaagtcc tgaaatcagc gcttcttcta acatttgacc tgagattctt
    28021 gtatcccgcc ctactaacac gcgtgggcgt tttccttctt cttcatgctg gcttaggaca
    28081 taacctccgc accgtcccag cttaaatgca agttctggcg taagttcctt gttggcttca
    28141 ccgcgtacgc cgtctgttcc aaaatattta cccatagttg taaaattact cctttaactt
    28201 aattttctga ggaatttgtt ctgctcattt gtgttgattc gcttgtttct gatgtttctt
    28261 ctgtacttgt ggtcgagctg cttgtcccag attccgaact ggaagaaatg gttgttgtcg
    28321 atggttccgt tcctgaatca gcagatgaaa tacttctact cgtacttgta ttttcttgtg
    28381 tccgactgct cgtcgaagca gagctactgc ttgacgtttg caacacgggt gtaatctcca
    28441 cactaactga ttcgggcttg atacttaacc cctcttctac tggtatttca attttacgag
    28501 ttgttcgatg attgatacct gagacattga tcggtacagc aatactgtca ccgatcgatg
    28561 caagcaaatc gctagagccg cttacttctg cttccagttt tgaaaaactg aaatcatagc
    28621 tttctacccc ggcagctggt gttccttgct gtatcccata aagtcgtacc gacttgcttg
    28681 gcttgatcga atcagctctc actgttactt gcactggatc agagacgata cttaatactt
    28741 cccctgctgc attcagcgcc tgtattggga cctcactatt aatcccatca tctgtgacca
    28801 tcgaagcgtc cacatcagct actactcgtt tgatttctgc caatgttttg tcacctgtag
    28861 tgatagagac ttttttagga ctaacggaag tatcatcaag ttcatatcca gatggtgtga
    28921 acgttgaagg aatcaacgtt tccacatcga aatcttttat tacctttttc tcaaccgtta
    28981 ctgtaatagt cgatggttcg atctttgctg tgacagcact gctcaaattt tgaattttta
    29041 gcggaacttc atgtgttcct tcgcccagct tagtgatatc tgctgtgacg cggaacatcc
    29101 gtgtatcttc gtttgcctct gcattcaact gaactcggtt tgcactggaa agtttgacag
    29161 tcactgcatt ttcatatcca tggatatagt agcgctttga atcataagaa atcttgatcg
    29221 gcacccccgt aactgtttga ttatacgctt catttccaga aagcgtattt ggaacggact
    29281 ggccatttgc attgaagtac agcgcaatac tgaacagcaa tgcaataaaa ctgtaaagaa
    29341 tgcttgtccg tttctctttt gagatcattt gcgatcccct cccttagtca taccgtctag
    29401 gaaagattga atcacgtttt tcttgttttt tgttttctct tcagcaatta gttctctacg
    29461 caagattgcc aaatattctt gctgggataa gccagccatc agttcattat ctaacgtgat
    29521 gctgacgtcc ccggtttctt cagagacaac gatggtaatc gcgtcactga cttcgcttat
    29581 accgacagct gcccggtgtc gagtcccgaa ttctttaggg atcaacatac tttctgatag
    29641 aggaagatag gcgcttgcta ctgcgatctt tccttctttt acaataactg caccgtcatg
    29701 cagcggtgta ttaggaataa agatattgat cagcagctcc ccagtgatat ctgcatctaa
    29761 agcaatccct gtctcaatat actcatctaa tccagtatga cgttcgatag tgatcaacgc
    29821 accgattttt cgcttggaca tatattggat ggctttgtca aaagacaaga tcatcttctc
    29881 atcttcctgc tgttcgcttt ttgattgttt gaaaagcgaa ctgcgtccaa gatgttccag
    29941 tcctctccgg atttccggct gaaaaatcac gatagcagct atcaccccgt acgtaatgac
    30001 ttggttcatc agccaagaca aggtgtgcag accaatgatt tccgctacga ttcggataac
    30061 aataaaaata gctacacctt tcagaagctg aatcgctttt gtgcctcgca gcagttggat
    30121 cagtttgtat acaagatacc agacaaccag aatatcaagt atgttcacca aaaaattcag
    30181 agagaacaag tcaccggaaa acatttgctg ccaatagtcc aaattaaaaa gttccccgat
    30241 ttgaaatgac ataaaaagcc tctcttctct tttgagattt cctctctagt atatcatatg
    30301 ttttaatttt agaggctgat atgctcgtat ttttcttgag aaaaacgcat aattttcaag
    30361 aaaattgcaa gtatgcgttt tatttttctc tgcaataaat atttttaaac ttcagagaat
    30421 tattcattat attttttctg ttatttttcc tttttttctt ttgtagcttg ttccattttt
    30481 ttacgtcgtt ctgctctggc agctcttcta gcagctcttc tttcttctgc ttcaaggtcc
    30541 gtcgtttttt cttttctagt cttaacgggt acatcgatag attcctctgg ttcaagtcgt
    30601 ttcttgattt tttctaattc tcccgtcagt gcagcatagt tgtaaaaacg tgttttagcg
    30661 tcgtggacag tcttttcaaa taaagctttt gcaagatttg gatttgcgtt atctagcgag
    30721 gaaaagcgaa cttgtttcct catgaaatca ggcatagcag agaaatcagg ttttttgtag
    30781 tccaaagtca ttgggttctt gtttttttcg accattgtag ggttatatcg atataatgac
    30841 caataaccag aagaaacagc ttcttttgct tctttaagcg attctttcat tccgccaagc
    30901 aagccatgtg tgatacaagg cgtataggca ataatcagag aaggaccagg atacttttct
    30961 gcttcttcaa atgccttgat cgtttgcatt tgattggcac ctgaagcaat ttgcgccaca
    31021 taaacatttc catatgtcat cgccatcatt cctagatctt tctttgaagc atactttcca
    31081 ctagccgaaa atttagcgat tgccgaagct ggtgttgctt ttgaggtttg accgccagta
    31141 ttggaataga cttcgttatc caaaacaagg atattcacgt ctgctccgct ggcaagtaca
    31201 tgatcgatcc cgccaaaacc aatgtcgtaa gcccatccgt ctccaccaat catccattgg
    31261 ctgtttttta cgaatagatc ctttctcttg tatatatcct ccagttcagg gaaaattgat
    31321 cgttcgcttt cgatcgcagc ttgcagtttt gctgctcttt gctgcgttcc ttcactctca
    31381 aacatgtgta ctaaccagtc ttccatcaat tctcgtagtt cttctgatac ttttggcaat
    31441 acttctttga ttgaacttgc caaagcctcg cgacgagtct gtgccgctag aagcatacca
    31501 taaccaaatt cagcattatc ttccaataaa gaatttgacc atgcaggccc ttgaccacat
    31561 tcattggtcg tataaggaga agcaggagaa gcaccgcccc aaatagaaga acagcctgtc
    31621 gcattcgcga tcatcattcg atcaccgaac atttgtgtca ataattttac ataaggtgtt
    31681 tcgccacagc ctgagcaagc gcccgagaat tcaagaagcg gttgattgaa ttgtgtagac
    31741 aagacagtat tctttccttt gactggattt gctttttgtc ttaatgtcat tgcaaaagcc
    31801 caattcacag cttgttcttt ctgttcttca taaggcttca tcactaaagc tttttctttt
    31861 gcgggacaag cctctacaca cagaccacaa cctgtacagt cttccactga aacttggata
    31921 cggtatttta cgccgtctgc gcctcgcatt tctctcacga taaatccttc tggcgcttct
    31981 tccatttcct cttcatctgc taaaaatgga cggatggcag catgaggaca aacaaaggca
    32041 cattcattac acatcgtaca gcgatcacta atccactcag gtacttctaa agcgacgccc
    32101 cgcttttcca tagaagccgt tcctaaaggc atttcgccag ttgtcattcc atttgctaca
    32161 agtgtaccaa cagagagttg atttccttcc tgtgcattca ctggttcaag tatttcttgt
    32221 atataaccag tgttcctttt attgattttt tcaggcatct ccaacgttct ccaatggtct
    32281 gggatcgcga cttgatgaag cagttcggtc gtccgatcga ttgcttgtat gtttttttct
    32341 acgatagcca ttgatttatg tccatagctt ttcagggctt cttcttttag cagcggaagc
    32401 acctcatcaa aaggaatgat gtcagctaat ttaaaaaaag ccgtttccat tgccgtattg
    32461 atccggcgtc ccaaaccgac ttcttgggcc aatctcatgg cattgatcgt atagaatcta
    32521 atgttatttt cagccaagta acgttttaat tttaagggca gatgcgtttc taattgttcg
    32581 tcgctccaaa gagtattcaa taaaaaaatg ccacctggct tcaatccttt gaccaagtca
    32641 tagctatgca ggtaagctgg tgtgtgacag gcaacaaggt ccgcatgctc gacaagatac
    32701 gctgaacgta tcggcgtatc tccaaaacga agatgagaga cagtcagacc gccagatttt
    32761 ttcgaatcat aataaaaata gccttgcgca tatttatcgg tgtgatctcc aataatcttg
    32821 atagctgatt tatttgctcc aactgttccg tcagatccaa atccccaaaa cttggcctgg
    32881 aatgtttgcg gttctgtcaa atccagactt tcctttggtt ccaaagattg ataagtcaca
    32941 tcatccacga tccctatggt aaaccgtttt ttgcgtatgc ttgggtcttt tttcaattca
    33001 tcaaaaactg ctgagatttg gtcgggagtc acatctttag accccaatcc gtagcgacca
    33061 ccaataacag ccgggcgaag ttcagaatca tataacgcac tttgaacgtc taataagaga
    33121 ggttctccac ctgcccctgg ttctttactt cgatcaagaa cagcaattgt ttttactgta
    33181 tgaggcaact tttgcacgaa tgtttccact ggaaaaggac gataaagatg gatattcaaa
    33241 aaacctgttt tccgtccgtt tgcattcaag taatcaatcg tttgttcaat cgtctgtgca
    33301 actgatccca tagaaacaat gacttcttcc gcatcgtctg ctccgtaata agtcaccaag
    33361 tcataagaag ttcctcgtaa tcggttgatc tctttcatat atttttgaac gatttcaggt
    33421 aatttatcat aataggggtt gatcgtttcc cgttgttgaa aatggatgtc tggattctgg
    33481 ttcgtaccac taacagtcgg atgattggga ttcatccctc gtttacgaaa tgaagctaat
    33541 ttctcctgat tgatcagagg agctaattct tcatattcca aaacttcgat tttttgaatc
    33601 tcgtgactcg tacggaatcc atcaaagaaa ttcaaaaaag ggacttctcc ttcaatagaa
    33661 gcaagatggg caacaggtgc taaatccatc acttcttgta cactgctttc agctaacata
    33721 gcaaatcctg tttgtcttgc agccatcacg tctccgtgat ctccgaaaat attcaacgca
    33781 tttgtcgtca ctgcccgact agcaacgtgg aaaacacttg gcagtagttc cccagcaatc
    33841 ttgtacatat tcggtatcat caaaagcagc ccttgtgaag cggtatatgt agttgtcagt
    33901 gcaccagttt tcaatgatcc atggaccaca ccagctgctc ctgcttcgga ttgcatttct
    33961 actactttta ctggttgacc gaatacgttt tcttttcctg ctgctgacca ttgatctact
    34021 aattcagcca tggtcgaact gggtgttatt ggatatattg cagcaatctc ggtaaatgca
    34081 taagaaatat atgcggctgc cgtattgcca tccattgttt tcatttttcg catgattcat
    34141 catccttgtt catattctct cttattatct gttccattat aacgtaaaac atgccatttt
    34201 tttcttttat ttataaaaat attttacatg tatctatttg aattggtcat ctaattttta
    34261 gacaaacagt ctaaatcgtt gccttttttt gtcagacatg tataaactat aagcgaaaga
    34321 ggtgttttaa catggatata tcagtgattg atgcaacgaa aacaaataca caaaaaggaa
    34381 tcctttatgg ttcatcgaat gctcctaaaa aaatggtcga attcatcaac ttagcttgtc
    34441 cgtattgccg acaatggttt gaagaatctt atgagctatt agaagaagca gtccaaagcg
    34501 ggcaattgca gcgagtgatc aaattgttcg ataaagaaaa agaaagctta cagcgaggga
    34561 acgtgatgca ccggtatctc acaatcagcg atggccaaaa agcgatcaaa gagatcaaac
    34621 aaatattcga tactcaagac gagtggaaac atctttctct ccaagaagta gcggattttg
    34681 cagtagataa actaaagctg acagaacaaa aagacgaaca gctgagccaa gctgtcatca
    34741 acgaagctga gcaagcacat attcgatttg ttccaactgt catcttgggt aaagaaatct
    34801 ttgatgaatc tatttcaata gaggaattaa aagaattgat ccaaacaaaa taaaaaaata
    34861 aggagcgtgg gacaaaagta aagaacactt tt
//
LOCUS       DARBDV010000017        34330 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000017 DARBDV010000000
VERSION     DARBDV010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.017"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            202..1077
                     /locus_tag="SCM56_000832"
     CDS             202..1077
                     /locus_tag="SCM56_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109654.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1222..1374)
                     /locus_tag="SCM56_000833"
                     /pseudo
     CDS             complement(1222..1374)
                     /locus_tag="SCM56_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1642..2973)
                     /locus_tag="SCM56_000834"
     CDS             complement(1642..2973)
                     /locus_tag="SCM56_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1109655.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(3122..3835)
                     /locus_tag="SCM56_000835"
     CDS             complement(3122..3835)
                     /locus_tag="SCM56_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1109656.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            4010..5434
                     /locus_tag="SCM56_000836"
     CDS             4010..5434
                     /locus_tag="SCM56_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG1109657.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            5733..6440
                     /locus_tag="SCM56_000837"
     CDS             5733..6440
                     /locus_tag="SCM56_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEG1109658.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            6427..6750
                     /locus_tag="SCM56_000838"
     CDS             6427..6750
                     /locus_tag="SCM56_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEG1109659.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(6839..7876)
                     /locus_tag="SCM56_000839"
     CDS             complement(6839..7876)
                     /locus_tag="SCM56_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEG1109660.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(8197..9180)
                     /locus_tag="SCM56_000840"
     CDS             complement(8197..9180)
                     /locus_tag="SCM56_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEG1109661.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(9595..10365)
                     /locus_tag="SCM56_000841"
     CDS             complement(9595..10365)
                     /locus_tag="SCM56_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1109662.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(10358..11293)
                     /locus_tag="SCM56_000842"
     CDS             complement(10358..11293)
                     /locus_tag="SCM56_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1109663.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(11609..13258)
                     /locus_tag="SCM56_000843"
     CDS             complement(11609..13258)
                     /locus_tag="SCM56_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEG1109664.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(13375..15978)
                     /locus_tag="SCM56_000844"
     CDS             complement(13375..15978)
                     /locus_tag="SCM56_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEG1109665.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(15990..17039)
                     /locus_tag="SCM56_000845"
     CDS             complement(15990..17039)
                     /locus_tag="SCM56_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HEG1109666.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(17065..17379)
                     /locus_tag="SCM56_000846"
     CDS             complement(17065..17379)
                     /locus_tag="SCM56_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HEG1109667.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(17395..17841)
                     /locus_tag="SCM56_000847"
     CDS             complement(17395..17841)
                     /locus_tag="SCM56_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1109668.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(17842..19764)
                     /locus_tag="SCM56_000848"
     CDS             complement(17842..19764)
                     /locus_tag="SCM56_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEG1109669.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(19976..20872)
                     /locus_tag="SCM56_000849"
     CDS             complement(19976..20872)
                     /locus_tag="SCM56_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG1109670.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(20865..21545)
                     /locus_tag="SCM56_000850"
     CDS             complement(20865..21545)
                     /locus_tag="SCM56_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1109671.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(21546..22328)
                     /locus_tag="SCM56_000851"
     CDS             complement(21546..22328)
                     /locus_tag="SCM56_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1109672.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(22321..23238)
                     /locus_tag="SCM56_000852"
     CDS             complement(22321..23238)
                     /locus_tag="SCM56_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1109673.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(23402..23725)
                     /locus_tag="SCM56_000853"
     CDS             complement(23402..23725)
                     /locus_tag="SCM56_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109674.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            24513..27740
                     /gene="fss3"
                     /locus_tag="SCM56_000854"
     CDS             24513..27740
                     /gene="fss3"
                     /locus_tag="SCM56_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HEG1109675.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            27853..28167
                     /locus_tag="SCM56_000855"
     CDS             27853..28167
                     /locus_tag="SCM56_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG1109676.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            28180..28554
                     /locus_tag="SCM56_000856"
     CDS             28180..28554
                     /locus_tag="SCM56_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG1109677.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            28564..28899
                     /locus_tag="SCM56_000857"
     CDS             28564..28899
                     /locus_tag="SCM56_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HEG1109678.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            28982..30322
                     /locus_tag="SCM56_000858"
     CDS             28982..30322
                     /locus_tag="SCM56_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEG1109679.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            30400..31074
                     /locus_tag="SCM56_000859"
     CDS             30400..31074
                     /locus_tag="SCM56_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEG1109680.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            31067..31231
                     /locus_tag="SCM56_000860"
     CDS             31067..31231
                     /locus_tag="SCM56_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109681.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            31307..32449
                     /gene="dcm"
                     /locus_tag="SCM56_000861"
                     /pseudo
     CDS             31307..32449
                     /gene="dcm"
                     /locus_tag="SCM56_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            32439..32729
                     /locus_tag="SCM56_000862"
     CDS             32439..32729
                     /locus_tag="SCM56_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109682.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            32988..34172
                     /locus_tag="SCM56_000863"
     CDS             32988..34172
                     /locus_tag="SCM56_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEG1109683.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            34169..34306
                     /locus_tag="SCM56_000864"
     CDS             34169..34306
                     /locus_tag="SCM56_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HEG1109684.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
BASE COUNT        10701 a         6609 c         6254 g        10766 t
ORIGIN      
        1 ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca
       61 cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc
      121 aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt
      181 gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg
      241 gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag
      301 tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa
      361 aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg
      421 gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc
      481 gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag
      541 attcttatag aacatccgca atttatcgaa cgcttcaaag ttaagatgcc atttcatgca
      601 ataaattatt cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa
      661 aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca
      721 gttgaatcgc tcattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg
      781 attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata
      841 gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg
      901 caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc
      961 tactccataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa
     1021 aaaatagata aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa
     1081 gtaaacgata acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca
     1141 cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga
     1201 ctattaggta cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctgggtagat
     1261 tttccggcat tgttgataga ttttatgata cttttctaca ttttgtaaga gaggttccac
     1321 tgcatctgta tattggataa aattctgaca acaactttca ggtgtgtcga accactctaa
     1381 tctttaaaga taaatattaa aataacgatt aataatatta acagtcttta tttttcacca
     1441 ccaaaaacct atctttgcat atactaaata gacgtatata cacagatagg tttgtcttgt
     1501 gaaaaataat gattatcctt accaaaaaat gtttgttttt ctacatgttc gtacttatca
     1561 aaaaatatga tccttattga aattactggc tatttatatt ccaacgactt cttgtgttaa
     1621 tttttgttat ctcatagtaa attatgtttc attttcactt aatttagttg tcacgtgttc
     1681 gtcaattcgt aaaaacggta aatatataaa cacacttgcg gctataatta atatttgtaa
     1741 aacagctgct cgccagtctc cggctgttgc taaaaatccc gatataactg gtggcgtagt
     1801 ccaaggaatt tgcactacta catgattaat taatcccaca gcagttgcaa aatatgctac
     1861 tgtcaaagaa aaaatcggtg caagcacaaa gggaataatc agactgatat taaatacaat
     1921 aggcaatcca aaaataatag gttcgcttat attgaatata gcgggagtta ccgataattt
     1981 agcaaagtcc ttataatctt ttctacgact gaagataaat attgcaatca ataaagcaat
     2041 cgtatttccc gatccaccca ttaccgcaaa ggtatcttta aaggccatat tgataatatg
     2101 tggaatttgc tctttgtttg cataagctgc catattttct tgcatattct gaatcaaaaa
     2161 cggatctaac aatgaacctg atattactgc ttgatggata ccaaatccaa aaaatagatt
     2221 agcaatagat gtaattaaca aaaatcctgg taaaccagtt gtaattttac ttaatggctc
     2281 tgtaactatc gtggtaatta gcttatttat atccatatta aacaattgaa tgattataaa
     2341 tgacaataaa gcaaacgagg taatcgtaat aatgattggg attaatacat taaaagactt
     2401 taaaacagcg ggaggaatat ttccggatag ctgaatctgt agtttcttat tattagaaag
     2461 tttaataaat aattctgtag ccgctaatcc aacaaatata cctacaaaca ttcctgaagc
     2521 tcctgtatag gatataggta tcacacccgt cacttcaaca gctttatccg cattttcagg
     2581 agtaaacatc atctgcatag gtgttaatac aacaaatgtc gaaatagcaa ctaataccgc
     2641 tgcaatatga tttgtataat ttctgcttgc acacatatga taagatactg ctgctacaac
     2701 taataatgta gttatgctta atgtaccatt ggcaattgaa gtaccaattt gttgtaacgt
     2761 ggtcaatgta ctagtatcga caatttcccc catgaatcct gttggttcta agatcactga
     2821 attaataagt gtcacaaatc ctgctaacat cataaatggc ataaatgtag caaacgcatc
     2881 tcgcaaagtt cttaaatgga tctgattccc caacttagtc gcaactatac caaatttttc
     2941 aaatacaatg tccttcctgc tacttttgcc catatttttt ctcctttatg cttattgtaa
     3001 tcgattactt tttccaccca aataatacat cacttatcaa aaaaagtaaa tacttttaat
     3061 taaatatata tatctttttt agtataaaaa aataagctct gaaaaaataa ttttcccgag
     3121 cttatttttc aacaaaaaca gcttcaaaaa caaagtcttg atataaatgt ctcgataccg
     3181 aatactcgaa aggtttacca gacgatagag caacaacttt gtctatctca atcataaatt
     3241 cacctggatc tatttttaat atttttgcgt caaattcatt cgcagtatct cctttgatac
     3301 gtatgtgcga agtctttatt tccagaccca gttcatttgt gatataagca tatacagatt
     3361 tctttaaatg ttttggttcc aagcctggta tgactgctaa tggcatgaat gtttgctcaa
     3421 tcgagtatgg ttgacaatca ataaaacggc ttcgtacaat attatataca tagtctttta
     3481 cactgatact taatttatca gctacttttt tcgatggaag ttcaattgag aaagtatgga
     3541 tttctgaatc tacgtgttct tcaccaacca cgctaaacaa accttcgttt ggaccaagaa
     3601 ctcgtgagtt atcaattggt gttctgacga cttcagtccc atacccactt ctacttcgca
     3661 aaactccttc ggaaacaagt aagtctacgc ctttctttac tgttaacttg cttgtatgat
     3721 aaataactgc caattcatct cctgtaggaa gtttctcacc aattttatac tctccattta
     3781 caattttttt ctttatatct aagtaaattt ccatatactt cgcaattttt ttcaatggtg
     3841 accctccaat caaaaacata tcttcatcta tactttaaca taaataaacc gataaaatcc
     3901 acatcaaaat tatacacaca ataaaaaaga tatatatatt taaacaaaac tatttacttt
     3961 aagttgtaag cggtgttatc attaagtgga aacaggaggt aaaaaaatta tgacaactat
     4021 gaaaaagcta acttttccag atgatttttg gtggggttcc gcttggtcag ctgagcaagc
     4081 agaaggaaaa ggagaaacag gaaaagcaga gactatctgg gatcgctggt ttaaagaaca
     4141 accttaccgg ttttatcaac gaatcgattc ctctattaca accgatcaca tccatcaata
     4201 taaagaagat atagaactaa tgaaaaaaac tgggcataac tcctttcgcg tttctatttc
     4261 ttgggcacga atgtttccaa atggcggtac cggaaaagta aatcccaaag ccatttcttt
     4321 ctatcgaaaa ttattttccg aaatgaataa taacggaatc aaagtatttg ccaacttata
     4381 tcattttgac atgcctgcta ctcttcaaga taaaggagga tgggaatcac gtgaagtagt
     4441 tgatgcttat gttcattacg ctgaaacttg ttttaaagaa tttggtgact tagtttgtca
     4501 ttggtttacc ttcaatgaac cacttgggcc tattttaggt acttatcttg aagattttca
     4561 ttatccaaac ctcattgact ttaaacgcgg agcgcaagca gcattcttca ctatacttgc
     4621 acacgcaaaa gcaatacaag tatttaaaga actagctctt cctagtaaaa taggcgttat
     4681 tctaaatttg agtcctactt ttcctcgtag tcaaaatcct gctgatgtga aagcagcaga
     4741 aatcgctgat ttattttata cgcggagctt tttagatcct atggttaaag ggaatttttc
     4801 taaaaagctt atcctgttat tacaagaata taatcagctt cctgaagatt atacgaaaga
     4861 agatcttgta tgtatctacg aaaataccgc tcaaattctt ggactaaact attacgagcc
     4921 acgtcgcgtc aaatctcgtt cttctatggt ggatacatct agtccatttt taccagagtg
     4981 gtttttcgaa ccacatatca tgcctggacg acgaatgaac gaataccggg gatgggaaat
     5041 ttacgaaaaa ggaatttatg atttgtgcat ggatatcaag aaccattatg gaaacatcga
     5101 agcatttatc tcggagaacg gaatgggcgt tgcggatgaa gaacgtttca tggatgatac
     5161 agggcaaata attgatgatt atcggattga gtatatcaaa gatcatcttg cttacttatg
     5221 gaaggcaata aatgaaggat gtaacattaa ggggtatcat ctttggacat ttattgattg
     5281 ctggtcatgg atcaacgcct ataaaaaccg atatgggctg gtttcactcg accttactac
     5341 tcaaaaaaga acaattaaaa aaagtggcga attttacaag caattaagtg atacaaatgg
     5401 atttagatac gatgtagact tgttagttga ataatattct aaaattccac ttgaagtgaa
     5461 cacattagaa aacaagaata atgagaaata ccaataacaa atactttttc ttcatacttc
     5521 atttatactt gtcagtataa gagactgtga caaatgtagt gatcctcaga agttaggtct
     5581 ggaaatctaa cttgagaaga tttttttaaa ctacgtccag tcttttttat ttgtttaaaa
     5641 ttgaagttga caagtcttca ttaaaaattt agaatattca caagtttgac gacaattatg
     5701 aaaatttatg tcgtgctaaa ggagttttgt tattggatga aaagttagat ataaaaactg
     5761 cgatcaaaga cacgttacct accgtttttg gttatatcgg tattggactt gcatttggta
     5821 tcgttgggaa agctgccggg tttcatccgc tggtcgtcac gttgatgtcc ctgctgatct
     5881 atgctggttc tgcccaattt atcacggtca gcatgcttgc tagccatagt ccactgctct
     5941 ccatcgttct ctcgaccttt ctagtcaatt cccgaatgat tctgatgagt atgacgattg
     6001 ctccttattt caagaaaaat cggctgcttc aaaatctgct gattggcaca ttgctgacag
     6061 atgaaagttt cgcactggga atgaataaat tgaactatac tggacaaaag ttaaactttc
     6121 gttggatgaa tacagctaat tggatttctt atttgacgtg ggtgggttct tctcttgttg
     6181 gcgcactttt agggaatttt attactgatc cgaaaaagtt tggtttggat ttcgccatcg
     6241 tcgctatgtt tatcgggtta ctttatcttc aagtcatttc tgatagaaac acctctaaac
     6301 gattacagtt gatcctgatt ggtctcactt tgattctggt atatgtcgga ctgattttta
     6361 tcccaagtaa cttagtgatt ttagttgtaa cactgattgg gtgcggattg ggggtgtgga
     6421 tcaaacatgc cttcttttga gtttacattg ctgactattt taggttgtac tttagcaact
     6481 tggctttcta ggatcctacc atttgtctta ctgaaaaaat tcgatttgcc gcaaccgttg
     6541 cttgagtatt taagttttgt cccgatcgtc attatgtctg ctttatggtt cagcagtcta
     6601 tttacccaaa atatcggaca tctgccgcag atcaatctcg aaaatgcttt ggcttcagtt
     6661 cctacacttt tagctgctat tctttcaaaa agcttattgg tgatcgtctt ggtaggaatt
     6721 ctttcccttt cgttgattcg cttattataa ataaaaaaac aagagcttat ggcaaattac
     6781 tgtcatgcgc tcttgttttt atcaaagtca aataagaaaa aaatcggcgt attcttgctt
     6841 atccgttgaa cggaccacta acaacgactt ttcctaatat atgtcctgtt tctacgtctt
     6901 tggttgcttg tttcaagttc ttcgcattca tcccatctga atagaccttg ctcaatgtac
     6961 tgcggataat cccttcattt gctaaagcag caatgtgcgc taaagcttct ccttgcgttt
     7021 cgatttcata gttatagtct gttttagcga acatatactc ccagtcgaag ctgatagatt
     7081 ggtttttcca aacacctata tctaacggct ctttgatccc tacgatcgtt cccacatgtc
     7141 caaatggtcg gacaaggtgt ttgatttgat caaagtagcg ggtaatatcg aataatacag
     7201 cgacatactc tacttgatcg attcctaatt cacgaaaact tttacttaag tcgtgatgat
     7261 aatcgattgg atagtctaca cctgtttttt tcagccattc aaaattcttc ggacttgcag
     7321 tcgcgtagac ttctaggcca gcccaatgcg ctagttgatt caaaatcgaa ccgacgcccc
     7381 cagctccgtt gatcactaag atttttttcc cgcggttggc attttcttct ggagtcagac
     7441 caaatttttc aaataacaat tcataagcgg tcaatgaggt caacggcaag gctgcggctt
     7501 cttcatctga aagatttttt ggtgcaagag cgacaatacg ttcgtcaacc agttggtatt
     7561 cttgattgct tcctgctctt gtcgttgtac ctgcgtaaaa aacgcgatcg ccgacagaaa
     7621 atttttgcac ttcatcacct acagcaacaa ctttcccgac tccatcaaac cctaagatac
     7681 gtaaagcatt tcttatcccg ttatcttggc gaagtttcgt atcgacagga ttaacagata
     7741 ctgcgttgac ttttaccaga agatcccgtg aactgggtac aggtatctgt tctttttcat
     7801 caataaagct ttttggatca tcgatcgaca acccttcata aaaaccgact accttcatta
     7861 attccttttc tgacatacga attcctcctt agcttcatca agttggagca tccatacagt
     7921 tttcatttca atatatatag tatgctattt gttccacttt tattcatcat aattattctc
     7981 ttacttaaag taaactattt gcactttttt ggttaaaaaa gaggttgcga caagactttt
     8041 gtcacaacct cttttaaacg gtgttcaatc aactgaccct cggtattcgc tcaaaaaacg
     8101 acaaaatatg aggagctatt tttgtttggc tgttcttatt acttgatgca ggacagcttt
     8161 ttcacgacct ctaattcacg gtgttcaagc aagaaatcat tgttcactga aaagcgttgg
     8221 atgttctttt tcaaaggtgt ctaagatctc tcgtacttca tcagtagatt tcgtattcat
     8281 caactgaact cgcaagtcat ttgctcctgg aaatcctttg atatagattt taaagaaacg
     8341 atgtagtcct gtgatggaac gcgggatgat ttctgaataa tgatcttgca gatctagttg
     8401 cagacgaagt agtccgatca gttctttttc tgtatgttcc ctcggttctt tttcaaatgc
     8461 ataaggattc ttgaaaatcc cccggccaat catgatgcca tctacgccat atttttcggc
     8521 caattcttct cctgtctggc gatctgggat atctccgttg atcgtgatca gtgtttgcgg
     8581 agcgatttgg tcacgtaaag caatgatttt agggatgact tcccaatgtg catctacttt
     8641 actcatctct ttacgcgtac gcaaatggac agaaagattg gctatatctt gatgaagtag
     8701 gtgactgatc cattcttcca tctcagacat ttccgtatag ccgatacgcg tcttgacgct
     8761 gacaggcaaa ccgccagctt tagccgcttc aatcaattgt gccgctactt ctgggcgaag
     8821 gatcaagcca ctgccttttc cacgttctgc tacattaggg actgggcagc ccatgttgat
     8881 atcgattcct tggaagccca tttctgccat agcaatgctc atttctctaa aaaattctgg
     8941 cttgtctccc caaatatggg caaccatcgg ctgttcatct tctgtaaagg tcaatcttcc
     9001 tcgtacactg tcttttccat ctggatgaca gaaactgtct gagtttgtaa actcagtaaa
     9061 gaagacatcc ggtgctgctg catgctttac gacatggcga aatacaacat ctgttacatc
     9121 ttccattggt gctaaaacaa aaaaaggctt tggcagctct ttccaaaaat tagtagtcat
     9181 ttatttgtta ctctccattc tatcaagcag tgaagtcgca atctttctca aaaacattgt
     9241 cattatacta ggaaacaaaa aaagaagcaa agtgaacagc ttaaaagatg tttctgattg
     9301 agcaaaaaaa gactgtgaca aaaatcttat cacaattcct tttccggaat aaatggtgtt
     9361 caaaagcttg catctgcggt aataagtcca accaaacgaa aaataggagg agctattttt
     9421 gtttggctgt tcttattact tgatgcagaa cagctttttc acaacctctc cactaaacgg
     9481 tgttcaatca gctgaccctc ggtattagct caacaaacga caaaatatga ggaactattt
     9541 tcccgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc ttttctaact
     9601 tctcaatcct cttcctttat tgatcaaata ccaagtaacg gcgtacaaaa caacgatgaa
     9661 tagcacaagt acgatcatcg atgttgctac tggcacatcg attgttccaa ggaatccgta
     9721 acggaaaccg gagatcatat aaacgatcgg attgactttc gaaacagctt gccaaaatgg
     9781 cggcaacata gagatggcat agaaaacgcc acctagataa gttaacggct gcaagacaaa
     9841 tgtaggcacg atcgatacat cgtcataaga ctgagcaaaa atcccgttga gcagtcctgc
     9901 taacgaaaac aagattgccg tcatcaataa tgtaatgatg accatgaacc atgaatacac
     9961 atgaagtggt acaaagaaca aggaaatgat cgttacgaga gagccgacga gaatactgcg
    10021 acccactccg ccaatcacaa agccccaaat gatcacatgc gtaggaacag gcgctaccaa
    10081 gatttcttcg atgtttttct ggaatttttg agagaagaag gacgaagaaa cgttggcata
    10141 cgaactagtg atagcagaca tcataatcaa accaggcacg atgaattcca tatatgaaaa
    10201 accacccatg tcaccgatcc gaccaccgat catttttccg aaaataacaa agtacaagga
    10261 tgtcgtgatc acaggcggaa ccagtgtttg gacccagata cgcaaatagc ggttcgtttc
    10321 cttgatagct aaacttttta atgcagtaaa ataaagatta aacatttttc tcctccgtat
    10381 gattattttc ttctgtgatt ttcaaaaaca gttcttctaa acgattcgac ttattcctca
    10441 tagaaagaac tttgattcct tgtcttgtca gctgatcgaa gattccgttc accccttgat
    10501 ttcgttcaac ttcgacagcg attgtctgtt catcttcaaa tgtacaagcg tatccttcaa
    10561 tcacaggttt ggtctcatat ggcgctaaat caaagataaa tgtttcaaat tgcagtttgg
    10621 atagaagctc tttcatacta gtattctcga tcagttcacc tgattggatg atcccgatgt
    10681 tgcgacaaag catttcagct tcttctaaat aatgcgtcgt caaaatgatc gttgtaccat
    10741 tttcgttcaa ttctcggaga aactcccaca tctcgcgtct caattcaata tcgactcctg
    10801 ctgttggttc atccaagatc agcaaacgtg gttcatgcat caatgcccta gcaatcatca
    10861 gtcttcgctt cattcccccg gaaagcatac gtgcacggac atttctcttt tcccaaagat
    10921 tggactgctt caaatatttt tcgctgcgtt tcatcgcttc tttgcgagag acaccgtagt
    10981 agcctgcttg attcacgacg atttgctgga ccgtttcaaa gggattgaaa ttgaactctt
    11041 gcgggactaa tccaatctgt tgctttgctc ggactagatc cgtatccaga tcataaccga
    11101 agacattcac ttttccagat gttttattga ctaaagaagt aatgattccg atcgttgtag
    11161 acttcccagc tccatttggt cctaatagcg catagaaatc tccttctttc acggaaagat
    11221 cgattcctcg caatgcttcg accccacttc cataaacctt tttcaaatta tttatttcaa
    11281 gcgcatttgc catttatttc ctatctcctt atagacatga ttctttattg tatcatggaa
    11341 tacagcagat gaaaaaacaa cttggttcca gcgaaaaatc aggttgctgg gctcaggaaa
    11401 atctcctaat gaaagacatc aatcggacga agtcagacaa ccttttcaga aaaaacaaat
    11461 cgaagataaa ggattactat tcatatgtgc aacaaaagga gcgtgagaca aaagtaaaga
    11521 acacttttgt ctcacgctct aaacacgtat aaacggcgga agcaaaagcc aagatttttg
    11581 atccaataaa tactttcgat cacttcgcct atcccaatac atgccactgc cgcttttcat
    11641 caaataacgc ggcttccgtt tttgcggttt ctactaaagt ctctgggtaa tccaaaactt
    11701 tcagcaaggc aatcgcattt cgggtccttg aaggtccttc ttttaatttg aaatcaaaac
    11761 tgattccttt tccttctgtt acttgttctt caaagtggac attctcacaa taattcttta
    11821 aaatctctgt caattcgata tcatgtgtgg cgacaaatgc taaagatgga tactccgcaa
    11881 gccaccgaac gactgaagcg gaagaagcaa tccgttcgat cgtatttgtc ccttttaaaa
    11941 tctcatctac gaagcagtag cagcgttctt tggaagccac ttggtccaac aaacgtttga
    12001 tcgatttgat ttctgaaaca aaataactgt cgccttcaaa taaatcatct tcgattgcca
    12061 tggaagtcaa cacatgtcca ggttgcaggg taaactgttc tgcaatcgct gtttggatcg
    12121 tctgtgctag aatacagctg atggctatac ttttgacata cgtagatttt cctgatgcat
    12181 tggaacccgt gactaatgta ttcttctgcc agttgaccgg attcactacc gcttctttta
    12241 acaatggatg atagatatct ttcgctgtca caccgccctc agtaaactct ggtcggcaag
    12301 tgatgggcat ataagtacgg taattcaata ttgcagcggc tacttctaat tctcccagca
    12361 attcccacag cttgatggct tcagttgaat gcttttctaa tttagtgaga acgaaatgat
    12421 aggagataaa tggcagcatg aacatgatgt tgacatattc aaaaatcaac tctgcttcac
    12481 tcccactctt catacgaaat gaaaaaccta atttagggat gtctcttaat ggtttagttt
    12541 gctgtttcag ctcttcttga agtggtgtcg gcagttttgc tagctgattg gcggctgaaa
    12601 tcgttgaaac caaataactc atgctattca gctctgtttc tagtgtttgt ttcttcacta
    12661 gataatagac cgtattgaat actaacccac cgattgccaa atagattcct agtacttgtc
    12721 ctaaaagaag caaacccaaa cccaccagcg gaagcaagcc aagaaaaata aataaaccta
    12781 cgcgtccaat ctttttactt tttccatccg ctagatattg ttttgcaaaa ttatgatctt
    12841 tttttcctaa acgagcgaat tgatattgga ttttttctct cagttgagga ttttcctgat
    12901 aaaaagcaat cagtttttct aactgctgat cttcaccaaa atcaaagctg cgtaaacgtt
    12961 gatacaacgc ttctgaacct acactagaat aggtactgtt gattccttcg aatacttcga
    13021 acatatccag gtcataccaa gtaagatcat cgatttcgct atcccatgaa cggaacttct
    13081 tctccgtgag ccatgcttcc ttcaagcttt cttctttatc aaatctcggt tgataaggaa
    13141 ttttttccca tttgctacga actgtttcct ttaatttcaa acgtgaaaaa atgtctaaag
    13201 caactaataa aataacagct gcaataataa ttaaaacgat aaattctctt tgacccatat
    13261 ccctctccgt tcctttattt gttagcttta gtatagcact gttgcgctat ttgaaaagag
    13321 ggtaaaaaaa cacctttcaa aataaaaggt gtttttttct tgtatattac atatttatct
    13381 ctcatttatc aaaattgaca aataatcata gggaggaatt gttgttaatt cttgtttatt
    13441 ctccagtgca ttgacaactg tcccaaggga aaggatagcc tcaggtacta ttttttcttg
    13501 ttctgttgga ttagctaagc gaagaacaaa tccttgttca tgaaaataag aaggataaat
    13561 cgatgataca attaagtctt caggtaattc aatcaatgaa aatttacgag gaacatcaag
    13621 ttcattttgt aatggccaaa tcttgttatc taacctgtga ataaatacat tcagtttctg
    13681 cttttgataa gaaatagatt gttctaaccg tttttctgcc tgttttgcca ctttgaactc
    13741 attaaatctt ccttcttcta cattaaatcc aaaagtgact ttatactcac ctatctcttg
    13801 agctagcggt gtttccatca tgacatgccc ttcatttgtc gtatctcctg aagctcgccc
    13861 aggtcgccaa gcaagattag gttttccaag ttcccctgtt gttgaaaata aggttatgat
    13921 taattgattt tctatacgtt catactcttt taaaccatct gcataaaacg taaaataatg
    13981 atcattttct gccacagaga cactcttatc gaatatttct aaattaacag gtttctctac
    14041 catagtttct gaccaattcg gaggaatgac atcaggggta ttttcaataa atccgttttg
    14101 aatttgtgca attgattttt cagatttatc taaaacatta attgctaagc gcatacgatg
    14161 cgagtaaaca gtattatgaa ttttgagtgt gccttcaacg atttgacttt ctcccagact
    14221 gaacgatatt tcataagata tttcttccat ttcgggtttt tcagataaac gatcttttaa
    14281 atctttagga agttgagctt ttccgtaaac aactaatgtt tcttgtaaag aatctttata
    14341 tactttagct gtttctaatt ttagagatag ttctgtatct ccttctaatg gtgaataatc
    14401 atacgtatcg ccatcatttg ctgaatcgat caaatgaacg aaatcaagat attgtctatc
    14461 tccaactatc aaattcaatt gcccatcttt aaaagaaagt ttatatttat tgttacttat
    14521 ctcagttttg tctatctcga ttgctgtttc taatttttca ttatttttct caaattcaac
    14581 aacagtgtat cctagtgctg gcacatttac atttattttt atgtcaagtt cataataagc
    14641 tggttcatca aaatattcga aacctgtagc aatcatccga taagcatttt tccgttctgg
    14701 aaaataaact tctttttcaa tgatattata gggactacct ttgaagtgta ttttctttga
    14761 atcagctact acatgcacga ttttcgatcc actataattc tcagtcgttg tgttgaaaac
    14821 aaggacttca ttcgatttta gctttaatgc atcagccatc cgttttacaa taaggttttc
    14881 gattccgtca gctatctcat tagcttcttt gattcgatgg aaaatatctt ccgcaacatt
    14941 atctgaaaca cagccaccaa tactatcatg agcctggttt tgcaaaatct ttttccataa
    15001 ccgacgaagt aggcctttac tgatatggat accatttctt tctgcaatca gcatgagcgg
    15061 ctcaactcgt tttagaattt tttgttccaa tttaaaattt tctattttca tttttgttct
    15121 tactgagcca atagaacgat gaacacgaga gtaaaccggc tgacgaagtt cacctttata
    15181 ttcaggtaat tcttttttgt ttctgataac ttctacataa gaaggataat ctgaaataat
    15241 attgtggaat ttactgtatt tattcaactt ctctacgact tgatcaaaat ttaaaatcat
    15301 tgacttttga tctacacccg aaggtactaa aatatctttg ttttttcctt gcatatgaag
    15361 catttcaact gcttcatcca acttttcttt tgcaaattca ggaatatctt taattgcgtc
    15421 taaggtcatc attccagtca tataaccaaa tggaaaattc atggcataaa caaatttatc
    15481 tcccaatcct ttccagataa aataaggaga tggaaccatc gtttcaaagt ttaatcctcg
    15541 ccaacacatg aaattatcga tttctacttg atgcaaaagt gtaggcaaat tcgcattgaa
    15601 cccaaacgtg tcaggcaaat accctaccat cattggatct ccatatttaa ggatcgtatc
    15661 actgattcca atcattaagt ttcttaaaat tgattcagca tccactaata aggcatctgt
    15721 ttgtgcatac cacggtccca cgaatagttg tcctgttttc atcagtcgtt ggataacctt
    15781 aagcttttgc ggattgatct ccaaatactc atcgacgatt gaagtttgtc catctaaaca
    15841 aaagtttact ttagggttcc tttccaattc atctagaact tcagtaaaga cctgatcaga
    15901 caatacgatg gcatcttcat ttgtaaaata ccattctctg tcccagtgcg tgtgttgaat
    15961 tatatgtgca tttaccatat cagattctcc tactttcctt cttctactga atttacttgt
    16021 tgttcttttt tatacttgat ggattttagt gcattgacca ttaacgctgt aaccactccc
    16081 ccaataacaa ttgatactaa atataaaggt atattcgtaa caaaaggaat tgccataaag
    16141 gttgaaagca ttgtttcttg agtaacacca aaaaatccat ttagaccaca tgctactgca
    16201 gtgcccacca cgatgcttgg tattactcgt aaaggatctt tcacggcaaa cgggatcgtc
    16261 ccttcagtaa tccccacaag gcccataatc atagctgtct ttgcattatc tcgttccgat
    16321 tcatcataaa gttttttgtt taaaattgtt gctaaaaaca ttccaaatgg cggaatagca
    16381 attcctaata acatggctgt actaggtcca aagcttccgc ttgaaaaaga agcaacccca
    16441 aaagcatatg cgattttatt gataggtcct cccatatcaa acgccatcat tcctcctaaa
    16501 atagccgaca tcaaagcagc atttgctcct gacatgcctt ctagccaatt agtcagtcct
    16561 gttgtcattg cttgaatagg cgtagctagt acataccata atatgattcc aacaactaat
    16621 gtagtaacaa aaggaatcag cataattgat tttagactaa taacttgcat cggtactttt
    16681 acttttttca atgcattagc taaatagcca caagctaatc cagcaaccat tcctccaata
    16741 aatcccatac caagattttg acacatcatc ccagtgataa aagctggtgc tagaccggga
    16801 cggtcagcaa ttgaatatgc gatataggct gaaatcatcg gtacgataaa ctgtaatccc
    16861 gtctgaccaa tcattcgcag gactccccag aatgtatccg tctggtccca aacacctgtg
    16921 ccaccaaaaa tgactgcaat agaagttagt aaccctgctg ctacgacaag cgggatcata
    16981 taggaaaccc cactttggaa atgatttaga atttctcttc cttgtttctt gaattccatt
    17041 agtaactccc ccacgatata ttttttattt gttaattaat gcaattgctt catcaatcgt
    17101 ctcttcacca tcttttacta cctctgaggt ccctactgtt aaaatcggtt taccgtcaaa
    17161 gcgacctcgt tctgaaacat ttgtatctac tgcaaatatg acaacatctg cctcggaaac
    17221 atccgaaatt gaaagtttgt tttctatccc catagccccc tgtgtttcta ctttaatatc
    17281 taaccctttc ttcttcgcaa acttttctaa attagctttt gccatataag tatgggcaat
    17341 ccctgcgata catgctgtta ctgctactat ttttgtcata tgaaattcct ccttttaaaa
    17401 tgatagagct tttaccaatt cttttttatt tctcgaatct tttattcttt taacaaactc
    17461 gtcatccatc aatcttccag ataattctgc taaaatacgt aaatgattag tacctttatc
    17521 agtgtctttt attgctaaca aaaagacgag atcaactggt tgatcatcta atgcgcccca
    17581 ttcaacagac cgctttaact tggctacagc cactgttgat tcaattactg ctaggctttt
    17641 gccatgagga atggcgatat ttttcccaat tcctgtggtc atatggtttt ctctgtcaaa
    17701 tacgtctttt tgaaattgtt tgtggtcaga cacaaaccca ttagtctcta acaatttcga
    17761 taatttttcg actacacttt ttttatcaga tgcatttaaa tctatcacta tattatttat
    17821 attgattttt tcactgatca tttataagcc ttctttcaat atataaacta atttttcaaa
    17881 cgacgaactc gtttttaaat ttgctaataa ctcatgattc gaagcgattt cattgaatgt
    17941 ttcataaatt ttattcaatt caggttttac tttttcatta agtgcaaaaa agaaaacaga
    18001 atgaacaagt gtcccatccc aatcaattcc ttctggatta acgtagacac agattactgg
    18061 cttttttata aaatctactt cgatatgcgg catagcaaaa tcattcatcg ctgttgaaga
    18121 tagtttttct cttttgattg ctccctctag tatcccttct ttcgcgtaat tttcttttac
    18181 caattttttg gtaattatat ttaaaacgtt cacttgatta tcctttattt ggtgggtaaa
    18241 aaagcaaaat cgtttatcta ataaatttag aaaagcatct tctgtttgtt catttaactg
    18301 tagatactgt tttacttttc tgacatctgc ccctaacatc aaaggactaa caacaataac
    18361 aggaaaattt gtattttcaa tcggaatcgt gcttataaca agttcatcag tgacttttct
    18421 atcttgtagt tcattgatgc ttagtacatc ttctatctca accatatctg caaaaccgtt
    18481 atttattctt tgctctaata attttgatgt tccatatccg ctactacaaa ctaatatcac
    18541 tttcgttttg tcaggtttgt aacgatccaa aagtgattga atatggagtg ttacgtaagc
    18601 gatttcatct tcattaaaat gtatacagaa tctctcttcg atttcctcta ataaatctac
    18661 acttatcata aatgaccttt taaaagaata tttgattttg tccgtataag ggttatagat
    18721 atttactccc aatttgagtc ttttaactgc tgcattgaga tgtaatacta gattattgat
    18781 caattcttca tcaggataaa attcatttaa tttaattctt actaactctt gtagctcaac
    18841 acccatatct aaataggaaa gcttcgtttg atctaagtcg tgattcatag catttttttc
    18901 aatcgaagta atgtgaatgt caacataagc ttgttcatct attggcacaa taattgaaaa
    18961 tgctttttca atcattttga ttaaaataag tgtattcttt gagatttctt cttcagaatt
    19021 attactacgt gataagaaat tgcttgtttc aatcctttct atcgcaatag caaggtggat
    19081 agctaaagtt tgaaattcat aatctgtaaa tttcaattgt gtcttattca tgtatcgatt
    19141 aagcaattcg gctacttttt tcaatatgtc cttatttaag aaattattcg cttcaccaat
    19201 gaaattgata ttcaagtcta agagatctgt tttactgtta gatgctgtaa agccacccca
    19261 ataatagttc acaacttctg acatcaacct tcttttactt ttttcatcag ctttgatgcg
    19321 cacgccattt cgatctgaaa ctaaggacat cccatgcttg tttagtcttt ttctaacatc
    19381 tttcattgtg ctttctaccg tcgacctgct cacgtataat tcgtcagcaa gattttgaat
    19441 ctttagagaa tcattagtat tcaataattt agcaaacaca taagttagac ggtcctccgg
    19501 agagtctgcg atattgattt tatgactgga taataaattt cgaagtgttt ctttttttcc
    19561 aataagcgaa acgccttttt taggttgaac caacatttgt acatctggca ttttttggat
    19621 tatttctttc aaaattaaaa tatctttgct aattgttttc gtagatacat ataattgttc
    19681 tgataattct ttgtaagttg tgtgctcatt ttcaatcaaa aagtatagta ttttcatttg
    19741 ccgttcgcta aattcgatgc ccattatctt cacctcctga atacagctta agtgtacaat
    19801 tattatctct attttcatac agaagaaagt tcttgattaa tgtagaagtt tctaatattc
    19861 ttgattattt caaatcaata aaaaagcctg caatatttat atcacaggct tttttctcta
    19921 ttaaatatat ataagataat acatttttct actagttctc actaatcttg tataattaaa
    19981 ccctccgcaa cttgacggag atggagaaga tgcccccgtc taatctagcg gtcatctctc
    20041 ctcccattcc ttccaccaat ttttttgaca ggtaaagccc gattccgcta gattccgtat
    20101 ttgtgcggct ggtattttcg gtataaaaac gctggaagac tttttcaaca gaggtttgcg
    20161 ttcctggttt gataggattg gccactgtta atcgaatgaa tggatcctct tttgaaaatg
    20221 tcacctttgc ttgtccatct gcataacgca ataaattctg tatgatattt tgcagcactc
    20281 gcagcaacat gagcggatct gctaatacag ttgtatttat cgagtgttca gcgaacgcta
    20341 cctcgatatt tttctcagaa aattgttcat aaaaagagaa aaatacttct tccacctctt
    20401 tagaaaaaga aagcgtatgg agatccattt ctttttgctt cgtctctatt cgagcaagat
    20461 cataaaactg atccgtcata ctgagtaatc ttgcaacaga ttgtttgatg atcgttaaat
    20521 acttctcccg tttctccgta tcctctacct gttccagtaa ttgtacataa ccgttaatag
    20581 cagtcaaagg agttctgaga tcatgagaaa ggccgacgat cgatagtctg atgttttctt
    20641 ccatttcttg aaatgcttgt cctttttgtt cattttcatc caaccatgta ttcagtgcat
    20701 ctactaggtc cattagattc ttttctcgaa attcgactga aagacgacca ccatattctt
    20761 gtcgttcaga aagagtggcg attttcttag ttaatctctt gatttccctt tgcaggaaga
    20821 aaataaaact tcccataaag ataacaacga gaagcaacag ccatttaatc aaatctaaac
    20881 cccactcccc acaccgtatg aatatacaca gcatgtgcct ttgctagctt ccctcgaaga
    20941 ttggaaatat gcatattcac tgttttctca ctatcaaaat atggttcttg ccaaactgtt
    21001 tcataaagat ttgctcgcga aaatactttt ttcggatgtt ctaaaaaaag cttcagcaga
    21061 tcgaattctc ttcctgtcaa atggacagcc acaccattca catttacatc cctcgactct
    21121 ttgtccagta agatttcctt gtaatagatg cgttgcgaca gatcttttac ttggacagaa
    21181 gtctgatgac gaagttggat gccgattcgt aacaaaagtt cttcgatatc aaatggtttc
    21241 gtcacatagt catccgctcc tgctgccaac aaggaaactt tatctctttg atcgcttttt
    21301 gctgaaagaa cgatgatcgg aacattcgat gtctgtcgga tttctgctaa caatgcttca
    21361 cctgaaagcc ctggaagcat gagatctaga aggatcaaat cgacaggata tgtgcttaac
    21421 aataattttc cttccgtacc tgaataagca tcaagaagtt tgtatctctg ttcaagaacc
    21481 tcctccagta aggaatgaac agcttcatcg tcttcgataa tcaaaatagt ttccagcagg
    21541 accacctact taatgtcaga tttttggaaa ataaacattc ctagagctga tgataccacg
    21601 attgttacta gactgaccag aatcattttc ggtgcttgcg aataagttgc ttgttgcgca
    21661 aagtccgtat acatgagcca cgtttccgta aaccactccg ttagaaattc caagcctttg
    21721 atttttgcga aaacaccggg aatgaaggcc gttcccatga tataaagaaa agaaagaagc
    21781 aaactgacac tggtacttct agtgatgaat gtcaaagcca ctggaacaga taatgtccct
    21841 aatgtcagca gaagcaatac aggaaatact tttagaacat aatttccata gtttttgatt
    21901 ccctcaactt caccaaatcc gaaagcaagt gtgtaagcaa gcatggctag tcccgtgatc
    21961 aaaaccacgc caagtgttgt gatcaaaagc gtaacgagaa gtttcgacac ataataagtc
    22021 attcgattcc gtcctaaaga taacgtattg cgaatcgtac cgttttgaaa ttcttcccca
    22081 atataaaagc acgcaaatgc tgcgaaagcc agaagaatcc atgcagccat tttttctacc
    22141 attaaaatcc ctgttgcacc gttgatgact gtatccgttg cttggtttaa ttcattgccg
    22201 tattttgtat gcatatacca ttcgttccaa gaataaaaaa gcggaagtac gaccatcagt
    22261 cctagtgaga cccacatttt acggctcttc catactttta ttttttcagc agttaataga
    22321 ttaaacatgt ggattgcctc cagttaattg taagaaataa tcttccagtt tttgcccaac
    22381 taggtgaata ccatccacag gaacttttgc ttctaccaat gcttgattga tggctgctac
    22441 ttggtcaatt ccttcaaaaa gctggataac acggctagat tgtacaaaat aatccctata
    22501 accaagagag tcgagaaccg ttactgcttg ttccgtcgcc gaagtgtcaa tttttataaa
    22561 ttgacgagat tcttgtgcca attcttcttt cgataattct ttgatcaagt gtccttcatg
    22621 caaaatacca tagtgagtgg cgatctgtga caattcatct agcaagtggc tagaaagcaa
    22681 gacggtgatg ccccgttctt ttaccaaacg ttggatgatt tctcgcattt ctgccatccc
    22741 tgctggatcc atcccattcg tcggttcatc caaaatcaaa aattctggat tcgtgatcag
    22801 tgcatttgca atagctaaac gctggcgcat acctaaagag aaattctttg ctttcttttt
    22861 ccctgtattt tccaatcgca tcattttcag caggtcttcg atttctctat ccgacacacc
    22921 gccgttcgct gcttggatac gtaggttatc tcttgcagtt aattctggat aaagagcagg
    22981 ggtttcaatc gattgaccca tacgtcgtct tgctgcctgc aaatccgtcg ttccaaataa
    23041 ttcgatcgaa ccttcatcaa tcgagatcaa acccataata gttctcataa aggtagactt
    23101 acccgctcca ttttccccaa tcaatccata gatcattcca cgtttgattt cgattgatac
    23161 tttatctaat gctttggcag caccgtattt tttggttata ccagttgctt tcaagaccgt
    23221 ttcatttcca aaatccatac ttatccttct ttcttattga tgagtgaagt atagttgacg
    23281 ggtataaaga aagcagccag aaatgtataa agaaagtgta aattttccaa ttgaatgtca
    23341 aaaaaaacaa tcggacaata cgtgtctttc ctattatcag gcagtttgtt atacgcttgt
    23401 atcagtttcc tgattctttc ttttcgacca aagtcgtttc tctgtattcc tcttcactga
    23461 aaatttttgc ttctttcaga tcaagcttga gtaattttac cagaccggtt ataaggataa
    23521 ctatagcaag actccctccc agaaaaatta gactgtctcg cagacgtggt tgcgttgtct
    23581 tccaatcaaa gagcgcacca aacagtgcta atccaagtgg actggccacc atggtaatcg
    23641 ttgttatcgt tgacataagt cgtccttgta tatgcgttgg aacttctcgc tgaagccaga
    23701 ccatataaaa agcaaaagaa aacattaatc cttaaaaacg gacttatgaa aaataataca
    23761 tgccgtttgg gatttacaat tagctaccaa ataagaggtt atgacaaaag acttttcaca
    23821 atcccttctt ccgaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat
    23881 ttatcaaaca tgagaagcaa tttttgtaaa ttccttctta tttcttgaag ctgaccactt
    23941 ctgtcacaac ctcagaagcg gataaataat attgtttgat gatactttat ctttttcccg
    24001 tttgtttata gatcgaatga tattatagac caagaatcct acctattaac atagctaaaa
    24061 acacaaaaaa ggcactttcc aaattgtgga aagtgccaga aaagttagga ttaaggtaac
    24121 tgcgctcagc attgcttcgc ccagtacgat tctcgtaact cgtgaactcg aacgataatc
    24181 gcaattaacg ttttgagaac tgtgaagctt tacgggcttt cttaagacct ggttttttct
    24241 tcgtatcatt gaataagttt gaaaaacctt gatttataaa ggttttcccg taaatatatt
    24301 ttattatatt tgattaaatt tgaaatgttt gtgttctaaa atgtgttcta atttaaaggc
    24361 agtatatttc gtattttaga ttaaattttt aggctcattc attctcaaat ccattctatt
    24421 cagtcaataa aaattttaaa aagactttat aaacaaagat gttcaagcga gcatcttatt
    24481 tttttgtgct taatttgaaa ggagaacatt tcatgtataa aaaacaaaaa aagaaattat
    24541 gtgtattaac tattttgagt ttggctgttc aagcaattgt tggagcaatt gctcctacga
    24601 ttacttttgc tgatgaaatt acccatccac aaacggtaac cgtagaacta gatttagcac
    24661 atcaatatgc tgttgaagga acttttagtg atggtcgtcc aatgtcagaa gtcactgttc
    24721 cgcattatgc agtgtacaac ggctttaaac aagatatttt ctgtattgaa ccaggagtcc
    24781 cgatttacaa tgagttcact cctggctatg agaaaaaccc gttaccagat atgtccgata
    24841 aagccaaatt agtttccgtt ttatggaaaa aagtaggtac agatgcagat actcatattg
    24901 tggcacagaa gatgatctgg caagaagtta atgggtatac ccttcactca ataaaacgat
    24961 cagatggtag tgccgtaaat atcgcagcca ttgaggcaaa aataaatcaa gccattgacg
    25021 attatcagaa gaaaccaacc ttccataata gtacggctaa aacagtatta gggcaatcga
    25081 ctactgtgac ggatacgaat aatctgaatt tgtcagagtt cgatgaggtt gtggaaaatg
    25141 ccgcaaacat tgattatcgt gtgaatggta atcagttgat aatcacccca aatgccaatt
    25201 caaaagaaag tggtgtgctg acgcttaaga aatctgctgg taccggaaca cctgtcgcct
    25261 ataaaaaggc tggccaacag actttaatgg ccggggcgat tgataaacct aatacctaca
    25321 caatcaaaat tgatgtggaa acggagggtt ctttaaagat caaaaaagtc gataaagaat
    25381 cgggtgatgt tgtaccagga acggttttcc atttagactt tggaaaaact ttacctgcaa
    25441 aagacgtgac tactgacaaa gaaggcattg ctacattgga tgagattcct catggtacaa
    25501 aagtaaccat taccgaaaaa tcagtaccgg cgccttatac gattgatacc acccccatga
    25561 ctaccaccat taaagcaggc gaaaccattt acgtgacttc aaaaaacgca cgggaaaaag
    25621 ggcaaatcat tctggataaa agcggagttg aaacagggag tgatctttgg aatgacaatt
    25681 attccttagc tggtaatacg tttgccattc gcaaagacag tccaaccggt gaaatcgttc
    25741 aagaaatgac cacggatgaa aacggtcatg cggaaacacc aaaagagatt gctaatgcgt
    25801 tggaattggg aacctattat gtgaccgaaa ctaaagctag tcatgggttc gtgaatacgt
    25861 tcaaacctgt aaaagtcgaa ttgaagtatg ccaatcaaac cgtagcccct gttaccagta
    25921 acgtaaaagg gcaaaatcaa gaagtcactg gcgaaaccac tttgacgaaa gaagacaaag
    25981 atactgggga taaagcacaa ggtaaggcag tctttgaggg tgctgaatac accttgttta
    26041 ctgctaagga tggaaaagct gttaaatgga gtgaggcttt taaaccagag ttagcgaagg
    26101 gaacgaaagg ttctgatgaa acggtcactt tgactttaga cgaaaagaac caagttgccg
    26161 ttaaacattt agctatcaac gagtattatt ggcaagaaac aaaagcgccc gaaggatata
    26221 gccttgatga aacaaagtat cctgtatcca tcaaaaaggt ggatgatagc gaaaaaaatg
    26281 ccgtgattac ccgaaatgtt acggcaaaag aacaagtgat tcgctttggc tttgatttct
    26341 ttaaatttgc tggatcagct gcgggcacta ccgaaactgg cttcaatgat ctgaccttta
    26401 aagtgtcgcc attggaaggg accaatgaaa tcacaggtgc cgaagatgaa gcgaccacag
    26461 cttataacga gcaattaggg tttgatggtt atggcaagtt tgaaaatctt ccttatgggg
    26521 attatttact tgaagaagta gaagccccag aaggttttca aaagattacg ccattagaaa
    26581 tccgttctac ttttaaggaa aacaaagaag actttgtgaa gagtgagtat gtctttacca
    26641 ttaccgaaca agaccaaaaa caaccaatta aaacggtgac cgttccttac gagaagctga
    26701 cgaacaaagc attttctgtt agtttgaacc gtttaatgct ctatgatttg cctgaggaag
    26761 aagatagttt aacttctctt gcgacttgga aagacggaaa caaagaatta acttctctgg
    26821 attctaccga gctagttgat aaattgagtt acaatctaca tgaaatcaaa gaagactggt
    26881 atgtggtagc gcaagccatt gatgtggatg cgacaaaagc tgcccaagaa aaagatgaaa
    26941 aagccaaacc agtggtgatt gcagaaacaa gcgcaacgtt ggcgaacaaa gagaaaacag
    27001 gaacttggaa aattcagcat aaattaaccg ccgaacaagt tttaaataaa actattgtct
    27061 tattcaacta tgtgtatgaa aataaggaag cttttgaagc aggcgataag ccagtagcga
    27121 aggatgttag cttgaataac caagcccaaa ctgtcagttg tacagtggaa caccatgttt
    27181 ctatccaaac aaaggcccac ctagaaaacg gttctcagac ctttactcat ggtgatgtgg
    27241 tggatatgtt tgatgatgtg tcgattactc atgatgtact ggatggctca aaagaagctt
    27301 tcgaaacaat tctgtatgct ttattaccgg atggtacaaa caaagaaatt tggaaatctg
    27361 gcaagattga ttatgaagta aatgataaag aattcaccaa aactgtgctt gcgaaaaaag
    27421 tagataccgg aaaatatcca gaaggaacca agtttacttt tgcggaaatc aattacgata
    27481 aagatgggaa tatcaatgga aaacacaatg aagatttgaa agaaaaatct caaaccttaa
    27541 caccaaaaga agttccaacc atactaagta cgccgaaaca accggaaaca ccaactgttc
    27601 caagtggctc tcaagaatct agtcccacag tgaagacctt ccctcaaacc ggggagaaaa
    27661 attccaatgt tttactgttc attggtttta ctttgatttt tgcgacggcg ggctattatt
    27721 tctggaatcg ccggaactaa ggtgatgaga tgatacaagt aaatcctaag aaccaacgtg
    27781 cgcgaccgcc tcctaaaaaa gtagtcgcgc tatttttgta tccaaaacta acaatgaaaa
    27841 gaggaaaaga agatggaatt gaaatttgtc gtgccagata tggctgaaac ctttggaaag
    27901 ttaagttatg ccggtgaagg agaaattata acagaaggat atggacggaa tactacggtg
    27961 attggtcgca gttatcattt gtattccagt aagcaacggg ccgatgatat tgaggtggta
    28021 gtagctgcgg aagccggtga aaaggatttt gatcaagacc aaccgctaaa agccgtgaat
    28081 ccccatttgg ttgccaaagg ctatgagatt gagaatcgtg ggtttactga ttacgtgttg
    28141 tatgttgatg atttagtgaa agcataggag gaaaagaaga tgagattaac agaaggtatt
    28201 gtcgtagatt caggtttaac atttgggaag ttgcgttttt ccgcattacg ccgagaagta
    28261 cggaagcaaa atgaggatgg aacaattggt aatgaggtaa aagaacgaac ctataattta
    28321 aaatcctctg cacaagggcg aatgattcaa gttagtatcc cagctaatgt acccttacgc
    28381 gagtttgctt atgatgcaga ggtagagttg gtcaatccaa tcgtggatac cgttgccaat
    28441 tatgtttttc gagaaggaac taccgttaat tggttcatta aggcggatga tttagtcttg
    28501 aagcggcagc cgaatcaagg aaatcctacg aaccaaaatg aaagaaagaa ataggtgaag
    28561 attatggaaa ttaatgttga acatatttta gattgtctcg atcaatatgg aaaaggcgag
    28621 ctgactgagg aacaactcgt taaagcatta acctctgatg aaaaaatgtt tctgattata
    28681 catcaaggtt tacttggaga aaatagcgac agtgaagaag actctgatgt actagactgg
    28741 ttaggagaag aagagtcttt ctttatagtt gtcgaagtaa atgaaaactt atgtcggcag
    28801 gcagaatcca ttttggaaga aattggtgtg gagatgccgg atgctattga aggcttttta
    28861 aatcaattgg ttgaaacgaa acaactcccc tcctcgtagc agtaaatgac tagcataact
    28921 ttttgaaatt gcctaatgaa atcctattgg gctttttcga ttggaggtgg aaaccatcaa
    28981 aatgtacaaa ggacatcgca tacgcgcagg agatcagcac ttggtttatc actttgttct
    29041 tggctggcta ctcgctctgt tcatcggttg gatgagcgtc ttttattttc aagagtttag
    29101 acaatttgat atttctaagc tatcactttc tactattgaa atcgtttggt ctataaaaga
    29161 tttagtatgt ctgcttggaa gtctcgcttt ttcaggagct atgatattgc tttatattca
    29221 cttttttctt gatcattggc gaagcttgtg gcatcgacaa aagctagctc ggatgattct
    29281 agagaaccat tggtatgaag tgaaacaaac tcaaagtgaa ggctttttca aagatttgaa
    29341 tagtagccga accagagaga ctatcagtta cttccccaaa atctgttatc gaatgaaaga
    29401 tggattactg tctattcgcg ttcaaatttc acttgggaaa taccaagatc agcttttaaa
    29461 attggaaaag aagttagaaa gcggactgta ttgtgagtta gtagaaaagg aactcaaaga
    29521 ctcctatgtg gagtatactt tactgtatga tatgatcgcc aatcgaattg ggatagacga
    29581 agtagtggca gaaaatggga ctctgcgatt gatgaaaaat caagtatggg cttatgattc
    29641 tttaccccat atgttaatcg ctggtggcac gggtggaggg aagacctatt tcctcctcac
    29701 cattatcgaa gcactattga agtcagatgc agaattgttt atcctcgatc ccaaaaatgc
    29761 agatttagct gatttaggta cggtgatgcc tcatgtttat tcacaaaagg aagaaatttc
    29821 tgcttgtgtg gaagatttct atgaacgtat gatggctcgt agcaaggcaa tgaaagaaat
    29881 gtctaactac aaaacaggag agaattatgc ctatcttgga cttccaccca actttttaat
    29941 ctttgatgaa tacgtggctt atatggagat gttaacgacg aaagaaagtg cggtgatttt
    30001 gaataagctc aaacaaatcg tgatgttggg tcgtcaatct ggtttctttc tgattttggc
    30061 ttgccaaagg ccggatgcga aatatttagg agacggaatc cgcgatcaat tcaactttcg
    30121 cgtggctttg ggtcgtatga gtgaactcgg atattccatg atgtttgggg aggtcgaaaa
    30181 aaacttcttt atgaagcaca tcaaaggtcg aggttatgtg gatacaggtg gcagtgtgat
    30241 tagtgagttt tatacgccac ttgtaccaaa agggtatgat ttcttagatt caattagtaa
    30301 attagaaaag atgaatgatt gagtgatctt caatactgac tttatacttc aagtaacgta
    30361 taatactttt atatagtagg aagaaagggg ataattaata tggaaagtaa tgattcttat
    30421 ttagagtatg caaaagcaaa taaggataag tttatttctt atattatcaa agggaaatcc
    30481 cccgaagttg gacgagacgc agtttttatg gcagggagtc ctggggctgg aaaatcagag
    30541 gttgccattg gattagctga gaattatgat aaccatgtga ttattgatgc ggattacttt
    30601 cgcacccagt ttcctgatta taatggaaaa aactctagtc tctttcaaaa agcatcttct
    30661 tggttggtag agcaggcatt tagatatgtc ttagatcatg gctattcttt tattctggat
    30721 gcaacttttg caatattaag tgctgaaaaa aatatctcgc gggcgttgaa aaaacatttt
    30781 cgagtgacga ttttctatgt ttatcaagac cctaaaatcg cttggaaatt tacacaagaa
    30841 cgtgaaattg cagagggacg tcatgtgccg aaaaaggcat ttattaacgc ttttttcaaa
    30901 tcaagagaga acattgcaaa agtaaagagt aaacatccag atgtattgct tcatatcata
    30961 ataaaagatt atacgaataa tatttccgaa gttcattatg ctacggataa tctagaatta
    31021 tttttaccac tagggtatag taggaaagat ttggaggttc aattacatga ctgagaaaga
    31081 acaagtaacg aaaattgtaa agaaatacaa taagagcatt gcggatttat cggaaaatgc
    31141 tactgcaaaa gagttcaaaa cggtgattaa atatgtagcc gatcaggcca atgaaaaaca
    31201 acggaaatta gttggattaa ataaaaaata gtaggacaaa ttaataatgt atgagccaag
    31261 agcaaaatgt tcttggcttt tttctttgca atgaatgagg taatgtatga gatttttaga
    31321 tttattcgcc ggtattggtg gctttcgttt aggtatggaa caggcgagcc atcattgtat
    31381 cggtttttgt gaaattgata aatttgctag acgtagttat aaagcaattc atgatacaag
    31441 taaggaggtg gaaatgcatg acatcacaag tgtatcagat gagtttattc aatccctcgg
    31501 accagtggac atcctttgcg gcggatttcc gtgccaagct ttttcaattg cgggaaagcg
    31561 gcaaagattt tccgatactc gaggtacttt attctttgaa atcgctcggt tcaccactct
    31621 tctccgacct aagcttttat tccttgagaa cgtccgagga ttgctcaatc acgaaggagg
    31681 ggctacgttc gagacgatcc tccgaacgct ggatggattg gggtatgacg tggagtggta
    31741 ggtgcttaac tcaaaggcct acattcccca aagccgggag cggatattcc ttatcggaca
    31801 ttctcgagac gcatgtaccg aacaagtatt tcctatcatt ggaccgtctt caacatctga
    31861 tcaaaacatc agaaatttgg taaatatcaa tccttccaat cgaggaatgg gtggacaagt
    31921 ctatggttca gatggcgtag cgccaacctt aacaggtgat gaaggaatca aaatcgcact
    31981 gccagcaagg gatggaatct ctgtagcagg actattaccg ggaaagtttg aacaaggtaa
    32041 tcgagtttat gaagttactg gaaccgcccc caccttgtca acgaaacaag gtggtacaaa
    32101 aatcatgatt cgaaaaaatg aaacagaata tcaagaggtg aagccagaag acagtgtgaa
    32161 tctggctttt cctaattcca caagtcgccg aggtcgtctg ggaaaacaga gtgttcatac
    32221 cttactaaca ggagaccaac aagcgatcgt cacggatcag tatcaaattc ggaagctaac
    32281 acctagagag tgttggcgac ttcagggctt tcccgattgg gcttttgatc gagattctca
    32341 agtcaattca gatagtcagt tgtataagca agccggaaac tctgtaacag ttccggtcat
    32401 ttttgatatt gccagaagat taaaagagtt ggatgtacat ggtttatgat gagttgattg
    32461 gagaaattta ttgggtgatt ggaaagattc agtcagaccc agaattagaa gaacaattgc
    32521 gacggttaaa atttgatatt cgcaagaatg gtgtcaaagt cgctggcgat ccatatttaa
    32581 tgaatgagga gacggatgct cgaattgaaa taaatcaagt aattgcagaa tttgagcgga
    32641 ttgcggacca agcaaaagaa ccagatattc gtcaatactt gttagaaatg aaagcagagt
    32701 tggaagtaaa tggtattaca gatgagtaac ctaaaccaac acaactgact gttaggcgct
    32761 gaccgcggag gattcgcgaa gcagaaatcc tccgtggtgc tgtgcctgtt catggacggc
    32821 gttagccgac catgatgtct cacccccgta tctaacaggg gggtacaaaa acacaaaaaa
    32881 taagtaagca accattaaga atgcttgttg tatcaaagat agtaagtaat ttgtataggt
    32941 gttaactcat ttgaaaaagt taacaccttg aatttggagg tgagaacttg gcacaacgga
    33001 atttagatta tcggctatta aaagaccgac gcaacgaata cggcgtttca caaaataaat
    33061 tggctacggc ttgtggactt agtcggccgt acttaaatca gatagaaaat ggtggcgtaa
    33121 ccgcatctat aaaaaccatg aggaaaattt ttgaccaact ggaaagcttt aatcccgatt
    33181 tgcctttgac gttactgttt gactatgtaa ggattcgctt tcccacaacg gatgcacgaa
    33241 aaattattca agaaattctc catttgaaat ttgagtatat gctccatgaa gattatgcct
    33301 tttactccta tcaagaacag tatgtcatgg gtgatattgt ggtgatgttg tctcatgaag
    33361 aagataaagg tgtgctttta gaattaaagg ggcgaggttg ccgacaattt gaaacttttt
    33421 tactcgctca aaagcgtagc tggtacgact ttttcgagga ttgtctaaag gccggtggcg
    33481 tgatgaaacg cttggacttg gcaatcaatg atcgagtggg gctattggat atacctgagc
    33541 taacgaaaaa atgtcagaag gaagaatgta tctccttgtt tcgtaccttt aaaagttatc
    33601 gttccgggga acttttaaag gctgatgaaa aggacggcat gggcaatacc ttatatatcg
    33661 gcagtctcaa gagtgaggtt tatttctgtt tgtatgaaaa agactatgaa caatatatca
    33721 agttagggat tccattagac caaactgaga cgaaaaatcg ttttgagatt cgactgaaaa
    33781 atgaccgtgc ttttcatgcg attcaagatt tgttaaaagg tcgtagcatt gagagcacca
    33841 cgttttccat tatcaatcgt tacttgcgat ttgcggataa agtagaaggt aaaaggcgaa
    33901 ccaactggcc cttgaatgaa caatgggggc gttttattgg acggaatcgg aaagagattc
    33961 aattaacttc cgagccaaaa ccgtatacca ttgaacgaac cttgaattgg ctaggccgac
    34021 aagtcgcccc tacgtggaaa atggctaagg agttggatcg attgaatcgg acaacctata
    34081 tccaggatat ggtacagaat gcacgcttat cagaccgaca taaaaagatt ttggagcaac
    34141 aaagtatggc aattgaaaat ttgattatat gaaaagagga atcgtcatgg gaattgtaaa
    34201 agatattttt ttggtatttg gtggcagtat gcttggtgtt actgtgatgt gcttgatgaa
    34261 tgcaagtgca gcagcggatc gagcaatgga aaagaaagga aagtgacaag atgaatttcg
    34321 gacaaaattt
//
LOCUS       DARBDV010000018        33419 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000018 DARBDV010000000
VERSION     DARBDV010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.018"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            317..1219
                     /locus_tag="SCM56_000865"
     CDS             317..1219
                     /locus_tag="SCM56_000865"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEG1109685.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            1432..3207
                     /locus_tag="SCM56_000866"
     CDS             1432..3207
                     /locus_tag="SCM56_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HEG1109686.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(3273..4859)
                     /locus_tag="SCM56_000867"
     CDS             complement(3273..4859)
                     /locus_tag="SCM56_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEG1109687.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            5110..5334
                     /locus_tag="SCM56_000868"
     CDS             5110..5334
                     /locus_tag="SCM56_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEG1109688.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            5476..7227
                     /locus_tag="SCM56_000869"
     CDS             5476..7227
                     /locus_tag="SCM56_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1109689.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            7227..9011
                     /locus_tag="SCM56_000870"
     CDS             7227..9011
                     /locus_tag="SCM56_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1109690.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            9337..10188
                     /locus_tag="SCM56_000871"
     CDS             9337..10188
                     /locus_tag="SCM56_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG1109691.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            10242..11993
                     /locus_tag="SCM56_000872"
     CDS             10242..11993
                     /locus_tag="SCM56_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1109692.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            11997..12167
                     /locus_tag="SCM56_000873"
     CDS             11997..12167
                     /locus_tag="SCM56_000873"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1109693.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(12207..13109)
                     /locus_tag="SCM56_000874"
     CDS             complement(12207..13109)
                     /locus_tag="SCM56_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG1109694.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            13340..14743
                     /locus_tag="SCM56_000875"
     CDS             13340..14743
                     /locus_tag="SCM56_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEG1109695.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            14743..16176
                     /locus_tag="SCM56_000876"
     CDS             14743..16176
                     /locus_tag="SCM56_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEG1109696.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            16321..17247
                     /gene="trxB"
                     /locus_tag="SCM56_000877"
     CDS             16321..17247
                     /gene="trxB"
                     /locus_tag="SCM56_000877"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEG1109697.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            17461..19188
                     /locus_tag="SCM56_000878"
     CDS             17461..19188
                     /locus_tag="SCM56_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEG1109698.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            19203..19553
                     /locus_tag="SCM56_000879"
     CDS             19203..19553
                     /locus_tag="SCM56_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEG1109699.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(19645..20505)
                     /locus_tag="SCM56_000880"
     CDS             complement(19645..20505)
                     /locus_tag="SCM56_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEG1109700.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            20597..21025
                     /locus_tag="SCM56_000881"
     CDS             20597..21025
                     /locus_tag="SCM56_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEG1109701.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            21187..21363
                     /locus_tag="SCM56_000882"
     CDS             21187..21363
                     /locus_tag="SCM56_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEG1109702.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            21390..21836
                     /locus_tag="SCM56_000883"
     CDS             21390..21836
                     /locus_tag="SCM56_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEG1109703.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(21990..23369)
                     /gene="brnQ"
                     /locus_tag="SCM56_000884"
     CDS             complement(21990..23369)
                     /gene="brnQ"
                     /locus_tag="SCM56_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEG1109704.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            24075..25046
                     /locus_tag="SCM56_000885"
     CDS             24075..25046
                     /locus_tag="SCM56_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEG1109705.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            25070..27262
                     /locus_tag="SCM56_000886"
     CDS             25070..27262
                     /locus_tag="SCM56_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEG1109706.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            27279..27755
                     /gene="ybeY"
                     /locus_tag="SCM56_000887"
     CDS             27279..27755
                     /gene="ybeY"
                     /locus_tag="SCM56_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEG1109707.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            27739..28134
                     /locus_tag="SCM56_000888"
     CDS             27739..28134
                     /locus_tag="SCM56_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEG1109708.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            28149..29048
                     /gene="era"
                     /locus_tag="SCM56_000889"
     CDS             28149..29048
                     /gene="era"
                     /locus_tag="SCM56_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEG1109709.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            29191..30003
                     /gene="recO"
                     /locus_tag="SCM56_000890"
     CDS             29191..30003
                     /gene="recO"
                     /locus_tag="SCM56_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEG1109710.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            30387..31304
                     /gene="glyQ"
                     /locus_tag="SCM56_000891"
     CDS             30387..31304
                     /gene="glyQ"
                     /locus_tag="SCM56_000891"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEG1109711.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            31306..33381
                     /locus_tag="SCM56_000892"
     CDS             31306..33381
                     /locus_tag="SCM56_000892"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEG1109712.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        11001 a         5920 c         7118 g         9380 t
ORIGIN      
        1 cttctattct gtttttttcg agtatttttg cgtggcaaaa gggtcacctc aactaatggt
       61 tttatttcgc taattatagc aaataataga acaaaaaaac agacaaggct gtttttggca
      121 ggaatttctt ttttaaaaga gttaaaacgt gagaaaatga tctttattat aaaagagttc
      181 ttaagctttt ctttagagaa aatttcagtt actatggaga acagggcaaa gaaaaatcaa
      241 tcattttctt tgtatatcat cttaccaata tataggaatt gttagtaaca attgcctttc
      301 aaggaggaat ccatccatgt tattaggctc acacgtcagc atgagcggaa agaaaatgct
      361 gttaggagca gcagaagaag cagccagtta tggatcttcg acttttatga tctatacagg
      421 cgcccctcaa aacactagaa gaaaagccat cgaagagatg aatattgatg ccggaaaagc
      481 gtacatggca gaaaaaggtc taacaaatat cgtcgttcat gccccttata tcattaattt
      541 ggggaatacg atcaaaccag aaatgtttcc ttttgccgtc caatttctga gagaagagat
      601 tgttcgtgcg gaagctttag gcgctactca aattacactt cacccaggtg ctcatgtagg
      661 ggcaggaaca gaagccggaa ttgctagaat tattgaaggc ttgaatgaag ttttgacaaa
      721 agaacagacg gcacaaatcg ctttggagac aatggcaggt aaaggtacgg aaatcggtcg
      781 aacatttgag gaattagcgc aaatcatcga tggagtaaaa ttgaatgaaa aattatccgt
      841 tacttttgac acctgtcata cgaatgatgc cggttataat attaaagaag acttttcagg
      901 tgtaatggaa gagtttgata agatcatcgg gttagatcgg cttaaagtac tccatatcaa
      961 tgattctaaa aatccgcaag gttcgcataa agaccggcac gccaatattg gttttggaac
     1021 aatcggattc gacacgctga ataagatcgt ccatgatcca atgtttgaca atatttcgaa
     1081 aatcttggaa actccctatg taggcaaaga taaaaagaaa gcttacgcac catatggaaa
     1141 agaaatcgcc atgttcaaag cgcaaacatt cgatccagaa gtgtttcctg agttattagc
     1201 agaagaagtg atactctaag attttctatc tgacagcagc aaaaaaggac caggcacatt
     1261 gccttgtcct tttttgttta agaaacaaag gagtactttt tgcagtcttt ttcatcatgc
     1321 atcttctccc ccctcttttt aaatttagac aaacaaggta tactggtaga aaagtaaaaa
     1381 aacgaaaagt ttcacaagca agtggcaaaa cgtgattgga aggacgaaaa gatgctgaac
     1441 actttaaaac ccttgatcaa ccaactaaaa aagaaacata catactttat tcttggcata
     1501 gccctattat ttacaattat tcgtttgtat ttatatagta aagcaatcta tggaacagac
     1561 atgtcggcaa cctatgatga ccagctgctg attcgctatg ctcaaaatat tttggacggt
     1621 aaatggctag gggagtatac gactacaaca ctttccaaag gaatctccta cagcttattt
     1681 ttagtacttg cacataaact gcatctttct tatgcagttt tgttcggcat gttcagtatt
     1741 tttgccagta tattggttgc actcgcgttt aaaccgattg tgaagaacaa gtatcttctt
     1801 ttaagtatct atttttattt tttgttttct ccgattagtt ttacacatga atattccact
     1861 cgaacatatc gtaatgccct cgtgattccg gctgttgaga tcattatcgc ttgtttatta
     1921 gctatttatt accgcaaata cggttctagc agaaaattgc tgccatggtt tttagggctt
     1981 tctgtcgctt ttcctttctt ttggttcatt cgcgaagatt ctatctggct tcttcctttt
     2041 actgtagtag ctttgggtat cagtttcgtc cagatcttgc tagggcaaga cttttctttc
     2101 aaagaagtaa agaagttaaa aaaacgactc tttcttctct cgaaacggga atggctgaaa
     2161 ctttcattgt ttttacttcc tatagttggc ttttccatcg tctatcaatg gatcgaatat
     2221 aaaaatgaga caacatacgg tattgcaacc atcaatgacc gttccagtgg agaatttgga
     2281 cgtttgatga aacaactgat ccgaatagat gatgaaacga acttgaataa agaaaacagt
     2341 gagatatggg tctcacatgc tgcactaaat aaagcattag ccgcatctcc tacattccat
     2401 actgtttcta cccgtattga cgaattatat acgactcacg cgtggactca aggtggaaaa
     2461 gtaaaagaat tacccggaga tatcatcttt tgggcattgc gagatgtggt aagcgaatct
     2521 ggttattacc aaaacaatgc aaaagaaacc aatgattttt ggaaaaaagt caaccaagaa
     2581 ttagaagaag cctatcacaa gggaacaatc aaaaagaaaa aagagttcta tctgatggga
     2641 tcaggagatg gaaagacgat gagtgatctg ccaatcgtat tcgatttcct tcaatcagga
     2701 tacgcttatg catggcgata taaagatttc catcaaggag gaaattatag tttcggaacg
     2761 caagaacaag tcaaacaggc tcaagaatta ttaaaccttc cgctaatgga gaattggatc
     2821 gatagtaatg aaccgagaac acctaatttc caattaacaa aatcagcaaa agtcgcgaat
     2881 gtcatcataa aaatttacca agttctggct attcccgtgc tgattgcttc aatagcaggg
     2941 atattactta tgatactagg aaccgtattt gcaaaagctg atcgtgccta ttatagttcc
     3001 tctttgattg tggtcaccgg attaatactg acacaattgg tttttctttt cggtgtctca
     3061 tggttttgtt cttatgcgcc agaaaaaaag gactattttt taagtgtcta cactggtgca
     3121 ggcgtgccat taacgcaatt catcgtagta acaagcatgc tagttttcag taaattaaat
     3181 ctgaaaaaag ctttatcttc taaataaaaa gacccttggt agctagattt ttaacctaac
     3241 tatcaagggt ctttgtgtgg aaaaatcagt ttttattcga tttcttctac cgtttcctgc
     3301 aaaagctgtt catatttttt gtttccttta tataaatcac caatcgtcca tgccaacagg
     3361 agaaggacaa gaacaatgat tacataagcg atgtgatcag ccaatgctag ttgagcggaa
     3421 gtggcttgct cgccgaagaa actttcgatc tgtcctggta atcctcgtaa gttcaagtaa
     3481 gttagactaa tcacagagaa ccagccaaga agttttacga tgaatgaatt tttaaatcgg
     3541 ctacccatct ccgcttggct gttggtcatc atcagtaaag gaatcatcga aaacggcaac
     3601 gcaaaagcca agaacacctg ggattcattc attaagttat tcaaagcggc atgttcttcg
     3661 atcgttcctt ttgtacttgt aaacaaaaca caaatcaata caggaatcac agaaagcaat
     3721 cgtgtgacca gtcttcttag ccaaatcggg atacgcatat ggatgaatcc ttccatgatg
     3781 acttgaccgg tcaacgtacc agtaatagta gagttctggc cagaagcaag aagtgctaca
     3841 gcaaataaag tggacaagat accagatttt gctacagcaa tcaaaatacc attgctcata
     3901 gctgatggat cagataaagc ttcatacaga ccaaaaaagg aaggatcttg gacagaaccg
     3961 gttttgaaga cagcgacacc catgatcaat agcagtgcat tgactagaaa agccattgac
     4021 aactggatat tagaatccca agtagaaaaa cgtacagctt gtgctacatc ttcttcgttt
     4081 tggtgatcga tcttccgagt ttgtgaaact gctgaatgaa gatataagtt atgcggcatg
     4141 actgttgcac caataatccc taaagctcca gtcagcggag tttcgccgcc gatgaccggt
     4201 gagtctgaga atgtatgact gttagggata aagccagcaa ataaactgtg ccaatctggg
     4261 ttagacaagc caacttgata gacaaaaacg aataaaatga cgaaaattaa gcagacaacc
     4321 agtgcttcga tcttacgaaa cccgactttt gtcagtagta agagaacgaa aacatctaat
     4381 acagtaataa agacggcaat caccaatgga atatcaaaaa gtaagtagag ggcgattgct
     4441 gcaccaatga cttctgcaat atctgttgcc ataatggcta attcagtcaa gatccataaa
     4501 atgacaccta atgtcttact tgtgcgtgct cggattgcct gtgccaaatc catttggctc
     4561 acgattccta attttgctgc catatactgt aaaagcatag caatcagact agacatcaaa
     4621 ataactgaca tcaataaata ttggaaattt tgtcctccgg tgatcgaagt agaccagtta
     4681 cccgggtcca tatacccaac tgctactaac gcaccaggtc cagagtaagc aagaagtgtt
     4741 ttaaaaaaac ttttcccctt tggtacatca actgttccat ttacttcagc taaagataaa
     4801 ccatttgtat gttcaataag tcgatgctta tgaaattgtt tattggattg attattcaag
     4861 aaaaaaccac ctttcctatt tatctacgat tacattctac acctcaaaaa gaaaaaatta
     4921 aagaaaaaga ttcggtacgc ctaatttttt ttcatggcgc gtcttgctag atgacgaaag
     4981 aaattttgtt ctcctctaaa aaaaccacta ttttttgcat gaaactaaag aatacgcctt
     5041 tcttttttca ataaacttta gtaaaataag tcattgagag caattcggaa atagaaagga
     5101 gagatttata tgttatcaac aggtattgtc gtacttattg ctattattgc tttgctaatc
     5161 ggcgcagtag gtggcttttt cctagcgcgt aaatatatga aagactattt agaaaaaaat
     5221 cctcccgtta atgaggacat gttgcgatca atgatgatgt caatggggca aaaaccatct
     5281 gagaagaaaa ttcgccagat gatgcagcaa atgaagaacc aagggaaaaa ataggttctg
     5341 acccaacgac tttttacggg ctgatttttt cagtccgttt tttatttcgt aatttgttag
     5401 actattcaga ttattcagca aaaaagacaa gcacagacaa tcctaggctc ttgataaaga
     5461 caaaggagga gtaaaatgtc gatttttaga aaattaggct ggttttttcg tcaggaaaag
     5521 aaacattatt taattggggt tttctcgcta ttcgcagtgg ccttggtaca gttagttcct
     5581 ccaaaagtga ttgggatcat cattgatgag atcgccgata aaaatttatc aataaagatc
     5641 attttcgggt ggatcttcct attgattatc gctgcttttt tacaatatct ttttcgttat
     5701 atctggcgga cgaatatttg gggaagtgca gctcgattgg agaaagaact tcggcagcag
     5761 ttattcaaac attttacaga aatggacaat ctcttttacc aaaaatatcg gactggggat
     5821 ttgatggctc acgcaacgaa tgatttgaat gcgatccaga atgtagcagg agctggtatc
     5881 ttgacctttg cagattcagt gattacgggc ggaacaacga tcattgcgat gattttgttt
     5941 attgattggc gcttaacact gattgcgctg attcctctgc ctttgctggc cgtcacttca
     6001 cgagtgctgg ggtcaaaact ccatgatgct tttcgtgatt cgcaagaagc tttttctagt
     6061 atcaatgata agacacaaga aagtatcact gggatcaaag tcattaaaac atttggacaa
     6121 gaaaaagaag atctagcgga ttttgaagaa aagattgaag atgcaattat taaaaacaaa
     6181 cgagtcaatt ttttagactc cttatttgat ccattcatta ccttgattat cggtctgtct
     6241 tatgttgcaa caattatttt aggtggaaag tatgtcttgg aaggcacgat ctcgatcggg
     6301 cagatggtct catttattag ttatatcggt atgcttgttt ggccgatgtt tgcgattggt
     6361 cgattgttca acgtattgga aagaggaaat gcttcttatg atcgcgtcaa tgaacttttg
     6421 catgaaaaga cgcatatcgt ggaacaactg ggtgcagtgc agaccccggc aaaaggcgag
     6481 ctgaatgtag ctatttcctc ttttaattat ccgaaagaag aatcaccagc attagaacaa
     6541 atcaagtttg cgatccaaga aggagaaaca ttaggtattg ttgggaaaac tggtgcggga
     6601 aaaacgacga tcatcaaatt actgatgcgt gaatacgatg attatcaagg gaaaatccaa
     6661 tttggcggac gttcgatcaa agaatattca ttgaatgctt tattaggatc aatcggctat
     6721 gtgcctcagg atcacttctt gttttcgatg accattcgag ataatatccg tttttctaat
     6781 ccaaaacttt cagaagaaga agtagagcat gcggcttctt tagcatttat caacaatgaa
     6841 atcaaggaat taccaaaagg ttatgatacc ttagttggag aacgtggagt ctctttatct
     6901 ggcggacaga aacaaaggat ttcgattgct cgagcattga tcaaaaatcc tgaattatta
     6961 atattagatg atgcactctc tgcagtagat gccaaaacag aagaagctat cttgcataat
     7021 ttaaaagaaa taagaaaaga gaaaacaact atcattgcag ctcaccgttt aagtagcgtg
     7081 atgcatgcaa aagaaatcat tgttgtcgat caagggaaaa tcattgaacg aggaacccat
     7141 catgagctgc ttgcactagg cggctggtat gaacaaatgt gggagaaaca gcaattagaa
     7201 gcgaagatag aaggaggcga ggcctgatgg atgaaaatta tcaatcggaa tggacaaaat
     7261 cgattccact taaagaacag aaaaagatca tcaaacgact gatgaagttc gccaaacctt
     7321 ttagaagaac gttcatcgtt gctatcttat ttgcgtttgc tttgtctgtg atcaatgtcc
     7381 tattgccaag gatcatccaa acttttatgg atgatcatct ggctaaacag tcggctacga
     7441 cacaagttat tttgtttttt gctggtatgt acttatttgg agtcatagta aaaagtatca
     7501 tttggttttt ccaatggtat ttgtattcca tggcctcatt gaagacctat cagtatatta
     7561 gggtaaaatt atttgaaaag ctgcatacac taggtatgcg ttattttgat cagacaccgg
     7621 caggatcaac cgtttctcga gtgacgaatg acacagaaac gctatttgaa ttttggtacg
     7681 tctttttgat ggtattgact gggatctttg cagtcgtttc ttcattcctt gccatgtttc
     7741 aaatcaatgg agaaattgcc ttgtggactt tggtgttttt gccgatttta ggaatcgtca
     7801 tctggtatta ccagaaattc agttctcgta tttaccgaaa tatgcgggaa aaattaagtc
     7861 agctgaacac gaaattgaat gaatcgattt ccggcatgcg tattatccag cagtttcgcc
     7921 aagaagctcg tttagctaaa gaatttgaac aagtgaacaa tgagtattta gacacgcgtt
     7981 atgcgatgat caaaacgaat tcgattctgt taagtccgat catcaatttt ctatatgcat
     8041 tggcgattgc gttggtgttg actttgtttg ggatcgatgc gttgcattct cccgtagaag
     8101 ttggactgat ctatgcattt acgacctatg tccagggatt ttttaatcca atgtctcaaa
     8161 tgatggactt tttgagcatc ttcacagatg ggatggttgc tggtagtcgt atcttgaaga
     8221 ttatggatac ggaagaatat gcaccacaac aaaaccctgg agcagatcaa atgattactt
     8281 caggtaagat tgagtttcgc aatgtaagct tttcttatga tggagagcac aatgtattaa
     8341 acaatatctc ttttgttgct aatccgggag agaccgttgc attagtggga cacaccggaa
     8401 gcggaaaaag ctcgatcatc aacgtattga tgcggtttta cgaattttat gaaggtcaga
     8461 tattgattga tgatcatgac attcgagaat atccaatgga agagttacgt aagaaaatgg
     8521 ggctggtctt gcaagatgct ttcttgtttt atggggatat cgcaggaaat attcggatga
     8581 tgaatccagc tatcacagac gaacagatac gccatgcggc tgagtttgtt caagcaaaca
     8641 aattcatcga tgaattacca aaaggatatc actcaaaagt gatcgaacat ggtgccagct
     8701 attctagtgg acaacgacag ctgatcactt ttgcgcgaac aatcgtgact gatcctaaaa
     8761 ttctagtatt ggatgaagca acagcaaata tcgatactga aacagaagga ttgatccaag
     8821 aaggattggc aaaaatgcgt caaggacgga caacgattgc gattgcccat cggctgtcaa
     8881 cgattcgaga tgcagaattg attttagttt tagacaaagg tcatatagta gaacgcggga
     8941 atcatgaaac acttctagcg caaaatggtt tgtatgcaga tatgtacaaa ctgcaaagca
     9001 gtggtatata aaagatgttg tgacagtaga attttgacct aagcactaga gtgaaaataa
     9061 aacatagctt ctcatatttt tcatctgtga gagttagtgt cgtagtcacc agatttaaaa
     9121 aaagattcga cagctagtca gaagtgccta atagccacta agaaagcgtt gacaaaagca
     9181 tttgctttcg ataatataaa gaaaacgata caacttggat agtgattgcc tgatgcaagc
     9241 taaacctgat gagtctccca tggtttcatg gacggctttt tgggtttttc tttttgtttt
     9301 ggatacagcc ctatacatca tggaaaggag caaaacatgc tgattgagaa agtactgaat
     9361 aacaacgtcg taataaccag ggatgaaaga aatcaggaaa tgatcgtgat gggaaaaggt
     9421 ttggcattca aaaagaaagt gggtgacccg atttttgact catgcatcga caaaaaattt
     9481 cagttgacaa atgacgggct ttcacaacag ttccaagaat tggcacaagc gattcctctt
     9541 gagtacttag aaatttcttg tgaaatcatt gaatatacaa aagaggctct acaagtccag
     9601 ttgaatgatt ctgtgtatat tgcattgacg gatcatttgt atacggctat cgaacgtcac
     9661 aaaatgggaa tatttgtacc aaatgtattg ctgtatgata tcaaacaact atttcctaaa
     9721 gagtatgcca tcgggaaaaa aacagtgaag aaaatctcgg ataaatacca catcaagtta
     9781 tcagaagatg aagccggatt tatcgcattg catttagtca atgcacaagg cattcaaagc
     9841 cagcaagaca atacctacct gtttactata acaatacagg atattctaaa tatcgtccgc
     9901 tattactata agattacatt tgatgaagat tcagtccatt tttttcgatt cgtcacacac
     9961 ttacgttttt ttctgacacg cgtcctagat ggagaaaaat cagaagtggg agaagtttct
    10021 gaggaattgt ttcaggttat tcaaaaaaaa tataaggata cgtttggttg cgttgagaaa
    10081 atagcagact ttttagccga gacgttcaac taccagatgt ccacggacga aaaaatgtat
    10141 ttaacgatcc atactgcgcg tttagtgcat gagaatcgtt gtaaataaaa aaaggaggat
    10201 aaattaacaa gaacgtatga aataagggta gtgacattca gttggctaaa ccttccacta
    10261 ataaattgga gggaatagaa atgggaaaat accaagaatt agcgagaaaa atcgtagaaa
    10321 atgtcggcgg gaaggaaaat atcaatggtt tgacacattg tatcacaagg cttcgtttca
    10381 aattaaaaaa cgaagaaaaa gcaaatgatg agatcttgaa aaatatggac ggtattgtaa
    10441 cggtaatgcg agcgggaggg cagtatcaag ttgtcattgg gaatcatgtg ccagtcgtgt
    10501 ttgaagaggt tataaaagca ggaaacctta cttttgatga ggctgtttca acagaaaaaa
    10561 tgagaccgtt tgatatgctt attgatggaa tatcgggctg tttccagcca ttccttgcta
    10621 tcctagctgc tggcgggatg attcgtggac tgacagcatt ccttgtcttc ttaggcgcgt
    10681 ttgaccgcgg ttctggcaca tttgttatgt ttgataatat cggtgattct gtattccaat
    10741 ttatgcctgt tattatcggt ttaacagctg cccgtaaatt taaagtaaat gaattcgtag
    10801 ggatgttgct tggcgcagca ttaatgaatc ctagcttgtc gctggaagct ttgagtggtg
    10861 cagcagaagc accactaacg accatatttt ctggcactat ttttgaagca cctatttatc
    10921 aaacagtatt cggcattccc tggattgcgc gtaactacgc ttcttctgtc attcccatta
    10981 tctttattgt gttgttagct agccaaattc agaaaccaat taaaaagtta gtacctgaga
    11041 tgatcgcgaa tttcttcgtt ccattcttca ccgttcttat tacaatgcct ttaggttttt
    11101 tactcgttgg accagtattc acattcgcaa ctgatatttt aatggctgga tttgaaacac
    11161 ttttagcact ttcgccagtt atttatggag caatcgtagg cttcttctgg caaattctgg
    11221 tgatgtttgg tctccattgg gcaatcgtac caatgggatt gatgcaattt tctgtgaatg
    11281 gttggcaaaa tattatgaca cctgttgcag tagtgtcttt tggacaaact gcagcactta
    11341 ccgcactcta ctttaagctt agaaatccaa aagacaaagc aattgcaata ccagccattg
    11401 tttctggtat tgtggggatt acagagccag ctatttatgg gtttacatta ccacgtaaaa
    11461 aaatatttat ttttacctgt gtcggtggtg cgttaggggg cgcgtatagt gggttaatga
    11521 atttaacttc ttggaatcaa ggtggactag gcatctttac aattcccaac tatattagac
    11581 cagatggtga tttaacagat gtgattaatg tattgattgg gatcgccatt gcgatggtgg
    11641 tatcctttac gctgactttt ttcttttgga aggatgaagc aggtgaaaca gatgatatac
    11701 agaaaaaatc aggtaaagaa attgtaaaaa caccgataca aggacaaatc gctccactaa
    11761 atgcagcaaa ggatgcagct tttgcacaag gaacactagg tcgaggaatc ttgatctacc
    11821 ctgaaaaagg agaagtacgt gccccttttg acggaacagt catgaccctg ttcccgacga
    11881 aacaggcaat tggaatggtt tctgaaacag ggctggaatt gctgattcat gtcggattgg
    11941 atactgtgca gttggaaggg aaatattttg aaagcttagt gcagcaagga taaagagtga
    12001 aacaaggcga tctgttgcta cgcttcgaca aaaagaaaat cgaaaaggaa ggatacagtt
    12061 tagaaacacc tgtgatcgtc accaattaca cggattattt ggatattttg gaaaaccaag
    12121 gggagacggt cgggccagac gatgcactta tcaccgcact gacctgaacg gttaggtgaa
    12181 gaatggacta ggcttaacta ttctttttat tccacttctt tattttcaaa attgaaaggc
    12241 tctttcatga tggctgagct gatagggcgt ttatcgaatt tttgaccgac agaaattaga
    12301 tggactaaat tctgttgata attttctgca aaaacacaac tatataacac gtctagaaga
    12361 taacaaattg atgtattgat cgtaaaatta ccaatttttg aatacagtcg ctcgcgtgtc
    12421 gtaagtcgca aagcggctgt actcattgca gatagcgttg aatcaccgat actggtcaaa
    12481 gctaaaattg gaacatgatt ttgtttcaat atttctgcgg tacgaaggat cacattgttt
    12541 tcacccgtat aagaaatcag aatcccgcac atatcactcg tgcaattgta cgcttcatag
    12601 aatgattcac caaaagtggt gacaatcgtg acatccattt tgatgcgctt cattttcagc
    12661 atgaaatctt gagaaatcaa agtattcgag ttactggcga aaatcacgat tttttttgct
    12721 tgaagcatta gttgtttggc tttttgcagt tggtcatgat gaatgagaga aagtgtatca
    12781 tgaatcgtca tctgttctaa agaagccatt ttatttgcga tcgtcataat accgtctgtg
    12841 gtggaaaaag gaagattcgc atctacgtct tgaaaattcc cttcaagata ggctgtttca
    12901 gctaaaaaag cattacgaaa actgacccag ccatcaaaac ccattttttt tgctacacgg
    12961 atgagtgtag aggggtggac ataacatcgc tcagcaatcg cttggatggt catttcgcca
    13021 agtgctggag aatcgcttaa gagaaactgg atcacttcat tttctgaagg agaaaaggag
    13081 atttctttga ttttttcacg taataacatg tagatacctt cttcttattt ttttacgtaa
    13141 attataactt ttttgaaaat gatttgtaaa cggatttata ctatataact taaaatagac
    13201 attttttgac aagatattta caaaaatcca ctagaaaaca gtaaattttg taaatgttac
    13261 tggaaagaaa cagatttcta ttgtttgttt tgtttacaaa aaagataatg aaagtgtaaa
    13321 aaagtaaagg agtgagaaca tggcaaaagg aatcaaaatc gctacgattg gtggaggcag
    13381 cagttacacc cccgagttga tggaaggatt tatcaaaaga tatgaggaat taccgatacg
    13441 agaaatctgg ctcgtagatg tagaagaagg aaaagaaaag cttgagatcg ttagtcagat
    13501 gtctcagcgt atgtgggatg cttctccgta tgaagtcaag atctatgcaa ctctggatcg
    13561 ggaagaagca ttgaaagatg cggattttgt tacgactcag tttcgtgtcg gtcaattaga
    13621 tgcacgaatc aaagacgagc ggatccctag ttattatggg atgattgggc aagaaacaaa
    13681 tggtgctggc gggatattca aagcttttcg taccgttcca gtcgttttgg caatcgttga
    13741 ggacatgaag cgtttatgtc caaatgcttg gctgatcaat ttcgcgaacc caagtgggat
    13801 ggtgacagaa gcagttatca gatatggcaa atgggataaa gtgattggat tatgcaatgt
    13861 gccagtgatg gcgatgatga cagaaccagc aatgcttggc aagaaaaaag aagagctgat
    13921 ctacaagttt gccggtctaa atcatttcca ttggcacaaa gtagcagata ataaaggaaa
    13981 tgaccgcacg gcagaattga ttgatcgttt atacgatgaa gagaatggat taccgaaaaa
    14041 tatctttgat atcccgtttt tcaaagaaca attaaaacaa atgaacatga tcccttgtgg
    14101 ctaccaccgc tattactatc gtgaagaaga aatgctggaa catgctttag aagaatatca
    14161 aacgattggt acaagagcgc agcaagtgaa agaaacagaa gcagagctat ttgaattata
    14221 caaagatcca gaattagacc ataaacctga acaattgcaa aaacgaggtg gcgcttatta
    14281 ttcagacgct gcctgtgaaa cgattgcttc aatctatgga aataaagaaa cacagatcgt
    14341 ggtatctaca aagaatgatg gagctgttcc tgacctgccg gcagattgtg tagtagaagt
    14401 cactgcctat gttggcgcac aaggagcgag aaatgtagcg tttggttctc ttcctgctgc
    14461 tgaaagaggc tggttgcaag taatgaagaa tatggaattg ctgacgatcg aagcagctgt
    14521 gactggtgat tatgggacag ctttacaggc gtttacgatc aatccactta tacgatcagg
    14581 aaagacagca aaacggatca tggatgagtt gtttattgca caccagcagt atttaccgca
    14641 attcaaggag acaatcagcc gattaaaaga agaaggaatc gaagttcaag atgaactggc
    14701 aagagaatta tcaggtaata cacatataat ggaggaagta taatgggatt cagaaaagat
    14761 tttttatggg gcggtgctac agcagccaat caatgtgagg gcggttatga tgaaggtgga
    14821 cgcggactag caaacgtaga tgttgtgcca atcggaaaag accgttttcc tgttatcacc
    14881 ggaaagaaaa aaatgtttga atttgatgag gatcattttt atccagcaaa aattgcaatc
    14941 gatatgtatc atcgttataa agaagatatt gctttgtttg cagaaatggg gttcaagact
    15001 tatcgaatgt ccatcgcctg gagtcgtatt tttccaatgg gggatgagaa agagccgaat
    15061 gaagctggct tgaaatttta tgaggatgcc tttaaagaat gccaaaagca cggaattgag
    15121 ccacttgtga cgattacgca ttttgattgt ccaatgcatt tgatcaaaga atatggtgcg
    15181 tggcgttcca gagaactcgt tggtttttat gaaaatctgt gtcgagtcat cttcaatcgc
    15241 tataaaggac ttgtcaaata ttggctgaca ttcaatgaga tcaatatgat ccttcatgca
    15301 ccattcatgg gcgcagggct gtattttgaa aaaggagaaa atgaagaaca aatcaaatat
    15361 caagcggcac atcatgagtt ggttgcaagt gccatcgcaa cgaagattgc tcatgaagta
    15421 gatccggaga acaaagtagg ctgtatgctg gcagcaggag catattatcc atacacttgc
    15481 aatcctgaag atgtttgggc ttctagaaaa tcagatcgag aaaattattt cttcattgat
    15541 gtccagtcac gaggagaata tcccgcttat gcattgaaag aaatggagcg aaatggcatt
    15601 cagatcgaaa tggaaccagg ggatgccgca ttgctgaaag aacatactgt tgattttatc
    15661 tctttttctt attatgcttc ccgagtttcg actaccgaca acgaactctt ggaacagact
    15721 gcaggaaata tctttgcttc tgtcaaaaat ccatatctgc aagcaagtga atgggggtgg
    15781 caaattgatc cattaggctt gagaataacg atgaatgata tttatgatcg ttatcaaaaa
    15841 ccattgttta ttgtagaaaa cggcttaggt gcagtcgata cgccagatga aaatggttat
    15901 gtggaagatg attaccgaat cgattacctg gcagcgcata tccaggcgat gaaggatgca
    15961 gtagaactag acggagtcga tctattaggc tatacgactt ggggatgtat tgatttagta
    16021 tcagctggta ccggtgagat gaagaaaagg tacggattca tttatgtcga tcgtgataac
    16081 caaggaaatg gcacattagc ccgttcaaag aaaaaatcat tcaactggta taaaaaagta
    16141 attgattcaa atggagaaaa tctttcgaac gattgatcaa tgatttataa aagtctattg
    16201 tttttcagcg gatttttcga gtataataaa gctattcgag aaacgcaagc agaacaatag
    16261 actttttttc gcattggctg taaagtatgc ttacttgatt aagtaaagga gaatctacat
    16321 atgtatgatg taatcgttat tggcgcaggt cctgctggga tgactgctgc tttatatgct
    16381 tcacgttcaa acttatccgt attgatgatc gaacaaggtg cccctggcgg ccaaatgaac
    16441 aatacggcag aaatcgaaaa ttatcctgga ttcgattcga tcatgggccc cgatctagct
    16501 tataagatgt atgaaagtgt ctcaagattt gggacagaaa atgcttatgg aatcgttcaa
    16561 gatatcaagg atcacggcag ccataaagaa gtcatctgtg aagataaaag ttacgaagga
    16621 aaaacagtca ttatcgcaac aggatgtatc caccgtaaat taggcgtacc tggcgaggaa
    16681 gaatttgctg gacgaggcgt ttcttactgt gcagtctgtg atggtgcgtt tttccgtaac
    16741 aaacgtctag tagtcatcgg gggtggggac tcagcagtag aagaagccat ctatttgaca
    16801 caatttgctt ctgaagtagt gatcgtccac cgtagagatg aattacgtgc acaaaagatc
    16861 atccaagacc gtgcatttgc aaatgaaaaa atttccttta tgtgggatac agtcgttgac
    16921 gaaatcgttg gaaacgaaat ggtagtgaca ggtgtgaaag cacgtaatgt caaaaccggc
    16981 aatgaatttg atttagcagc agacggagta ttcatctatg ttggactgga tccattaact
    17041 gcacctttta gagaagcagg tttgacgaac gaagctggct ggattccaac agacaacgaa
    17101 atgaaaacga atatgccagg cgtttttgcc atcggagatg ttcgtgaaaa agatttgcga
    17161 caaatcacta cagctgttgg tgacggcggt attgctggac aacaagtgta taaatacttg
    17221 gagtctttag aagaaacaac agcataagtt taaaaagagt agaagaacgg gttcaattgt
    17281 tcttttactc ttttttttcg cgtgaaattt actgaatctg atttatgcga acacgtccaa
    17341 caccttttga taaaagaatc ctttcttgat ttaagcgttt tttggtcctc atactttcat
    17401 atctaataag tgaatgctat acttattaag aaccatattt cagaaagagg tgttattttc
    17461 atgtcttggg aacaagtgta tgaacaatgg gtacatgaag aaaatttaga agaaaactta
    17521 aaaaaacaat taaatgatct gggtgaagat cctgaaaaac ttgaagatgc tttttatgca
    17581 ccgttggaat tcggtactgc aggtatgcgg ggaatcttag gacctgggat caatcgaatg
    17641 aacatctata cggtaagaca agcaacagaa ggactagcac gtttcatgga tacacaaggt
    17701 gaagaaacaa aacgccgagg tgtcgctatc gcttatgact cacgccatca gtcaccagaa
    17761 tttgcaatgg aagcagccaa aacactagca catcacaata tcccgtctta tgtattcgaa
    17821 agtctgcgtc caacaccaga gctttctttt gcagttcgtc atttgaagac gtttgcggga
    17881 atcatggtaa ccgcatctca caacccagcg gcttacaatg gatataaagt ctacggagaa
    17941 gatggcggac aaatgcctcc tgcagacgca gatgcattga cgaaatatgt ccgtgaagtc
    18001 agcaatccgc taaaagtcga agtattatct gacgaagaag ctaaacatag cgggttgatc
    18061 acgatcatcg gagaagaagt ggacgctgct tatctagaag aaatcaaagc agtgacgatt
    18121 gatcgtgaat tggttgaaac aatggggaaa gatttgaaac ttgtgtacac gccacttcat
    18181 ggaacaggga aaatgttggg cgaacgtgcg ttgaaacaag ctggattcga acaattcgta
    18241 ctagttcccg aacaagctgt ggcagatcca gatttcacta cggtcaaatc gccaaatcca
    18301 gaagaacatt cagcttttga atatgctata cgacttggag aaaaagaagg agctgacttg
    18361 ctgattgcaa cagatcctga tgcagaccgt ttaggcgcag cggttcgttt gccagatggt
    18421 tcttatgaag tattgactgg gaaccaaatc ggagcattgt ttgctcaata tattttggaa
    18481 gcacatcaat caagaggcac tttaccagaa aacgcagcgg tattgaaatc aatcgtttcc
    18541 agcgaattgc ctacagcaat tgcggaacac tacggtgcta ccatgttcaa tgtgttgaca
    18601 gggtttaaat tcattgctga aaaaatccaa caatttgaag aagaccattc acatactttt
    18661 atgtttggat tcgaagaaag ttatggttat ttagttaaac cttttgttcg cgataaagac
    18721 gcgatccaag cattgctttt attagcagaa gtagcagctt actataaaaa acaaggcaaa
    18781 acgttgtatg acggcttgca aacgattttt gaaacctatg gctatttcgc agaaaaaacg
    18841 atttctgtga caatgagcgg acaagaaggt tctgcgaaaa tctctgcttt gatggaaaaa
    18901 ttccgtaaag aagcaccgac agaatttgcc ggagcaaaag ttattcaaac agaagacttt
    18961 aaagaattga cgagaacaga ccgtgaagga aacgtagaaa aactaacaac accgccatcc
    19021 gacgtattga agtatacatt ggaagaaaat ggctggatcg ctatccgccc ttcaggaact
    19081 gagccgaaaa tcaagtttta tattggtgta aaaggaagct ctacagaaga cgcagccgaa
    19141 aaagtagcct cttatgaagc gattatcaaa aagatgacag aagaataaaa aacttgagac
    19201 tgatggaaaa agtggtaaac tttagaagtc agtctttttt caagttggag gtgaatggat
    19261 tgccagacac gcaaaaatca aaagtagaga aagtcagtca attattcaaa atgttgagtg
    19321 atcccactcg tcttaaaatt ctgctatatc tgaaagacgg ggaacaaaat gtgacagcaa
    19381 tcacccaagc agtggaaatg gagcagtctg ctgtatcgca tcaactacgt ttattaagag
    19441 aaaatcatgt cgtcaagtct catcgcgagg gaaaagcgat tttgtatagt cttgatgatt
    19501 ctcatgtact ggatattttg aaccagacac tcaaacatgt ggaacatcaa taacgatagg
    19561 tgaaaataga taaacactgg agtgtgacaa cgaagaaatt tcgttgtcac actccagtgt
    19621 tttttaagaa aaagacaaaa atgattaatc gcttttttct gttgtttcag ttccataata
    19681 actatgatct tctaggtcta aagcattcaa aataagcgaa gccgtttctt caatagataa
    19741 agatgccaca ttgatcacta cacatcctag tttttcataa agatcattcg caaaatcgag
    19801 ttcagctcta attttttcga tatcggaata agcagtatca gggtttaatc cgtaagagcg
    19861 catccgttct ttacggatat tatttaaaat atctgggtta ttcgttagtc ccacgatttt
    19921 tttaggatcg atttcccaca gctgtttggg aatatgtgct tggggaatca aaggtaaatt
    19981 agcaactttt aaatttttat ttgctaaaaa caagctcaaa ggcgttttgg aagttcggga
    20041 tacgcctagc aaaacgacat ctgcttctaa aaaaccacga ggatctttcc catcatcgta
    20101 ttttacagca aattccattg ctttgatccg tttgaaatag ttctcattca ataaatggag
    20161 ggcaccaggt tgccgaattg gcttgatacc agttaaacgt tcaatttctg atatcggtgg
    20221 cgtcatcaca tcgaaactaa ataattgatg ttcttcacaa aaagaacgtg ttatctcgat
    20281 cagttcttga ttgattaatg tatgcaggac aagtgctttt tcttctaatg catcttgcaa
    20341 tgcctgcctt aatgtttttt catctgtcac gaacgttctg cggaataggt tgaactcaac
    20401 ggtaggatac tgagccatag tagcttgcgc tagttttgtc gcagtttctc cagcagagtc
    20461 ggagatgaca aaaaaagtaa cgatttctgt agaatcggaa gacatggata aaacctcact
    20521 tgcatttttt ttatgtttat tataccatac tctagaatgc gtacttcgta atcattttga
    20581 tttggaggaa taagtcatga aaaaacagac attgatcgaa caatcattag aaacattgaa
    20641 aaataacggt tttaaatata ctaaaaaaag agaagcattg ttaacttatt tagtgaagcg
    20701 taaccgctac gtatctgcta aagaagtttt tgactatatg aatcaggaat tcaaaggggt
    20761 aagttatgat acgatttacc ggaatctcca tgattttgca gagatcgatt tactggaaga
    20821 aaccgattta aatggtgaaa tgaagtttcg ttttcattgc tgtcaggggg aaatcggcca
    20881 tcaccatcat tttatctgta cagtgtgcgg aacaacgaaa gaaatccata tgtgtccaat
    20941 gaattatttc gaagaacagc taggtggatg cacgatcgaa gggcatagat tcgagatttt
    21001 cggaagatgt gaaaattgtc aataaggatt taaataaaat aacatttgat aatttaaaaa
    21061 aaaagcaaga tttccttgac ggtcaaaaca gcattagata taatatttac aggacagtct
    21121 tgttttttga gatatatttc aataacaatt acggttactt tttaagctcg gagggaggga
    21181 attaacatgt caaaaacagt cgttcgcaaa aacgaatcac ttgacgatgc tcttcgtcgc
    21241 ttcaaacgtt ccgtgtcaaa agcgggtact ttacaagaat ctcgtaaacg tgaattctac
    21301 gaaaaaccaa gtgtaagacg caagaaaaaa tctgaagcag ctagaaaacg caaaaaattc
    21361 tagttctgtg atgaattgga gttggtagtg tgtcactact aagtagatta aacgatgaca
    21421 tgaaagcagc gatgaaagcg aaagataagg aatctttaca agtcatccga atgatcaagt
    21481 catctataca aaacgagcag attaaagaag gtcacgactt gacagaagaa gaagaactaa
    21541 cggtgttgtc tcgcgaaatg aaacagcgac gtgattcttt gcatgagttt gaagaagcag
    21601 gacgtgacga tttagctgaa aaagtaaaaa gtgaaattgt aatagtcgaa aaatatatgc
    21661 cggaacaact ttccgacgaa gaaattcgac aacttgttca agaagcaatc actcaaactg
    21721 gtgcttcatc aatgaaagaa tttggcaaag ttatgggtgc aattatgcct aaagtcaaag
    21781 gaaaagcaga tggaaaccaa gtgaatgcga ttgtgaaaga actattacaa tcttaatttt
    21841 atcatttaaa ctgcggacag tgattgtccg cagttttttg tgttcgtaaa aaaagaacta
    21901 aaaaacagct agaagaaaga ataagattct ttcttctagc tgtttttttc ctgtttaata
    21961 tctcttatgt taaattgtgt ttgatttggt tattcttctg gtagttcatt tgcttcttgt
    22021 ttataccctg ataggcgata ggcgtaataa aagagtaagc tgccagcgta accaagtaac
    22081 gttgggacca tccagccaag tcctaatgca gcaaaaggaa cgtgatgatg ataaaatcct
    22141 agtaagcttg ctacgacagt ttgctgactc actaatgcag gcgaatttgc caacatatcc
    22201 aatacggctg gaacggcaga aaagaacatc gttacttgat agacaaatgg actcttttgg
    22261 aagaattttg ctgttaagga aagcaagatt aacgcaagtg acaatggata caaaagcatc
    22321 aatactggta aagaccattg gatgatatta tccaatccgg cattcgcaac gacaaaagag
    22381 acaaaagtcg ttaaccgcag ccatgtcata taactgactt tagggaacag tttatggaaa
    22441 tcttgtgcaa aagaaacaac gagtcccata gcggtagtga agacacctaa tgtgaccatt
    22501 acccctaaga agctagcacc gaagttacct aaataaaaat tagtgatctg agaaagtgca
    22561 gttccgccat tttctgataa aggcatctta ttcaaactca tcgcaccgag aaggaccaga
    22621 ccaaaataaa tcatgacttc taacgcaacg ctcaggctac cagcttttgc tgttaattct
    22681 gtgacttttt tatctttaaa accaagatca cgaactgcat ggacaaaagt gacacttaaa
    22741 gcaagtaaag ctactgcatc aaccgtgttg tatccttcta aaacgccatt tgtcaaagcg
    22801 ttcgtctgat aagaatctgc agcagattgg tttagattcc ccatgggttg gaataaagaa
    22861 acgacaaaaa tgatagctaa taatgcaaga aacaaggagt ttaaatactt tccgacatat
    22921 ttgaccaaac gtgttggatg tacagtaagg aaataagcaa gtaaaaagaa aagagcagag
    22981 aataaaaaca tacctaatga atcatatttt tcaggcaaaa agggtttagc agccatttca
    23041 aaagcagttg ctgctgtccg cggtgtacca aagaatggtc cgatcgttaa atgaacaagg
    23101 atcaagaaca aggcagcata ccattttccg acaggtttag ctaagtcata cagaccatca
    23161 ctttttgtca ctactacagc taagatagct aataatggga aaagagcgcc tgagatggca
    23221 aatcctacac cagcgctgat ccagtttgat ccagccattt gacctaagtg gacaggaaag
    23281 atcagattac ctgacccgaa gaacatcccg aagataagtg aactgaccat caataattgc
    23341 ttccatgaat aagtttttgt ttcttccata aatttttcct ccaataatta tcattttgag
    23401 taacaaaata aacgttgcag aaactagaaa tgccactaat atcagattcc atgaaaacca
    23461 ccaccttaaa atataaaaaa aagccctttt ccacttggga aaagggctct agattctaga
    23521 acggtaccac cttttttcgc ttaaaaaagc ttcttcattc acggacgctt ttcgataaag
    23581 ttatccgcta gcctgataat ggaggcagcc acaatgatct acgttttcga cattgactcg
    23641 gaaatgatgt tcgtctattc gatttattgt catttctcac ttacatgact cactgggaat
    23701 aaatcgctga atagcctact gttttcctcg acgctttgct gtatatttat ttgttgacgt
    23761 tataatacac ctgttttttt tgaaatgcaa gaatatttca aatattttgt tttctttttt
    23821 tgaaacaaga aaaataccca gagaatactt gctaagttag acggaatact tgtcctctat
    23881 cttatgcata gcttacatga tcagcttttt ttattcaaac gagaccttta gagaatggaa
    23941 gcagctattt tttaacgttt cttggtactt atggttaagc aactctttta gaaccttttc
    24001 ttatttgcta tatagtttct tctgagtatg gtaaaatagg aaagaatgaa tcgaaaaaaa
    24061 ggggatgtct atttttgacg gaagagtcta gttctttaga gattagattg acggctgctg
    24121 atgatgtcag catgctttta ggtgcacacg acaagcatat aaaactgatt gaagaaacaa
    24181 cagaaacaac catccataca cgtggagaaa tcattcagat cattggcgaa caatcccaga
    24241 tttccttggc tgcctcagtc attcggacat tgcaggaatt gatcaaacga ggaatccatg
    24301 taagtacacc agatgtcgtg acggcactga aaatggggca aaaaggcaca ttagattatt
    24361 ttgttgaaat gtatgaggaa gagatcgtca aagaccgtaa tggaaaacca atacgggtaa
    24421 aaaacagcgg ccaaaagaaa tatgtcgaat cggtagccaa acatgatatc gtatttggcg
    24481 taggaccagc cgggacagga aaaacttttc ttgcagtagt attagcaatc gctgccttga
    24541 aaaaaggtga agtgcaaaaa atcattttga cgcggcctgc tgtggaagca ggggaaaatc
    24601 taggctttct tcccggggat ctaaaagaaa aagtagatcc ttatctgcgt cctgtttatg
    24661 atgctttata ccaaattttt ggattagatc atacgaaccg attgatggaa cgaggcgtga
    24721 tcgaaattgc tcctttagct tacatgcggg ggcggacatt ggatgatgca ttcgttattt
    24781 tggatgaagc acagaacacg acagttgctc agatgaaaat gttcctgaca cgtcttggtt
    24841 atcaatcaaa aatgattgtc aatggagata cgagtcagat tgatctgcct cgagggacaa
    24901 caagtggtct tgttcatgca gaacgaacat taaagcaaat caagaaaatc gattttgtta
    24961 attttgaagc aagtgacgtt gttcggcacc ctgtagttgc cgaaatcatc aaagcttatg
    25021 aaaaggcaga tctccatcaa gaataagctt tgcttaggag gataagcaca tgttgaataa
    25081 aacactgcag aaactacaag aaaaacttgg aaaggcagca ttgcctttcc tgctgttctt
    25141 gttttttatc attatctgcg gcgttacatt tagtagtgtc aaacaaaagg ccaatgattt
    25201 cagagaaggc caagtagcag aagaaagtat ccgtgcgaac aaaacgatcg agaacacgga
    25261 agaaaccgaa caaaaaagaa aactggcagc agaagcagtc actccagaat acacttacca
    25321 aaaagatctt gcagatgacc agaacaatcg gatcaagcaa ctatttgaat tgatcgataa
    25381 aacgaatgat gccatcaaca aatcttatga cgaaaagatt gataaggcaa aggaagatga
    25441 aaatatccca aagcctacta cagaagagcg gatcgccgcg ttgaagaaaa attttgaaaa
    25501 tgtgaatgca gaaaatgttg cgttttatca aaaactaccg aacaattttt attcgacgat
    25561 atttgaaatg actgaaactg agatccatac tgtgagggat gagagtctga agctcattga
    25621 cgaacagatg agcaaacaag ttcgtgaaag tgaattggaa gcttttaaac aagaagcaga
    25681 agagcaaatc caatatttga atgtcacacc agcacaacaa caaatgattc gctacttagt
    25741 ggatcaaggt atcgtagtga atgacgtatt gaatgagaaa aagacagaag aattgaaaca
    25801 atccgcacga gaagctgtac aaccagtgat gatcttccaa ggagaaatca ttgttcgtga
    25861 aggtaatcaa atcgatgctt cagctatgaa aaaattggaa ttactaggac tgactaatca
    25921 aacaacttcg atttttccgc taattgccat ggttttagct gttttgcttc aaatcggcgt
    25981 actgatttat aattcacttc aattccaaaa tgcaggagaa agaacaaaat acgtgctttt
    26041 ctatgtgaca gctatgtcag tcagcatagt gctgatgaaa tttttccaat tattccaaac
    26101 ggaacaagct acttatatcc cgctgtttta tcccgctgct tttgcaccac ttgttttaag
    26161 ctttttcttg aaccgaagag cagggatcat ggctggtatt ttccaagcag tatctgcact
    26221 tttcattttt tatgattcga ttggcacgaa ctttctaacg attatcttga tgtcttatgt
    26281 tttctctggg ctcatggcaa cgattgtcag acgcaaacgg atctcagaac aaggattatc
    26341 tgctgccatg tgggtcatta tttttcctgt gatgatggat gtcattttga tcgtctatca
    26401 aggaatgagc tttggtgatg caacgacttg gttaatgctt gtctgcgggt tcactggtgc
    26461 actcttctca ttcttgctga cgatcggtct acacccatat attgaattga tggtcaatga
    26521 tgacagtgta attgtattaa atgaactcag caatccaaat catcctttat tgaaacagtt
    26581 gctggaagaa gcgccaggta cttatcatca tagtatgatg gtagctaatc taagtgctaa
    26641 cgctgtagca gaaatcggcg gacgttcttt gctaactcga gtagcttgct attatcatga
    26701 tatcggcaag atcaaacatg ctagcttttt cgttgagaac ttgccttcag gagcggaaaa
    26761 cccgcacaat ttcttattgc cagaagacag taaacatatt atctttggac atgtgacaga
    26821 tggagccaaa atattggaag attataatat gccgcaaatg gtgatcgata tttgcaaaca
    26881 gcatcatgga accacgctaa tgcgttattt ctacatcaaa gcgaaagaac gcaatccgga
    26941 agtaacagaa gagcaattcc gttatcctgg accaaaaccg caaagtcgtg aagcagggat
    27001 cgtcaatatt gctgacagtt gtgaggctgc agtacgggca atggatcatc cgacgagcga
    27061 aaaaatcgaa agttttgtcc ataatcttat cgaagagcgt atttccgatg gtcaattaga
    27121 cgatagtgga ctgaccttga aagaaatccg aaaagtggaa aaatcattga tcagcggttt
    27181 aagtagcacc ttccattctc ggatcaaata tcctaaaatg aaaacagaag ttgagaagat
    27241 aaaggaagaa caagaagaat agaaagaagc gatgtataat ggatattacc tttatggatg
    27301 aaacaaacca agtctcagaa gagaaaatga aagaaatcga tgacttgctg caatttgcag
    27361 catcttattt aaagctgcca gaggaaacag aaatgtctgt tactttcatg gacaatgcgg
    27421 caatccaagt aatcaatcgt gattaccgtg gaaaagatgc accgacagac gtcatcagtt
    27481 ttgctctgga agaagaaggc gaagacgaaa tcccagtgat ttttgaagat ggagagaatc
    27541 cattgcctcg tgtactagga gacatcatga tttctattga acgtgctgct gaacaagcag
    27601 aagcttacgg ccattcatat gatcgggaac ttggcttttt agcggtacat ggattcttgc
    27661 atatcaacgg gtatgaccat atgacaccag aagatgagaa agaaatgttt ggtttacaga
    27721 aagagatctt ggatgcctat ggactcaaaa gataaaaacg tagagaaaaa caaacatttc
    27781 ctgatgtctg tggaatttgc tgtccaagga gtcaaaaccg tttttgagga agaacgcaat
    27841 atgaggaaac atgttgcgtt cggtatcctt gctttattta tgggcgtgat tttccagctt
    27901 gatcggatag agtggttatg gctgctttta gctgtttttc tcgtttggat agtagagatc
    27961 atcaatactg tttttgaaaa tgtagtggac atgtttacgg attttcattt tcatccgatt
    28021 gggaaaaaaa tcaaagatat ggcagctgga gcagttttag tgaccgcttg ttttgctgtc
    28081 atcattgggt tgattttatt tgtccctaaa atatatcatt tgttgtttaa ttaaaaagga
    28141 gaacgtatat gccagaacat aaatcagggt ttgtcgcaat cgtcggccgc ccgaatgtag
    28201 gaaaatccac attattgaac cgaatcgtgg gtcaaaaaat tgccattatg agtgacaagg
    28261 cacagacaac tagaaataaa atccaaggag tatatactac accagaagct cagctgattt
    28321 ttatcgatac accaggaatc cataaaccaa agcatcgtct aggagatttt atggtagaag
    28381 cagcttacag tgcgttgaaa gaagtggatg cagtcttatt tatgatcagt gctgatcaaa
    28441 aacgcggccg gggagacgat tttattattg aacgtctaaa aaacgtgcat tctccagttt
    28501 atctgattat caataaaatc gataagatcc atccagacga gttgctggga atcatcgaag
    28561 attattcgac gcagatgcct tttgcacaag tgattccgat ctctgctaca gaaggaaaca
    28621 atgtcgaacg cttaatggat gtattagtca gtgaaatgcc agagggccca caatattttc
    28681 cagatgatca agtgactgat catcctgaat actttatcgt ttctgaattg atccgtgaga
    28741 aagtcttgtt tttaacacga gacgaagtgc ctcattctgt cgcagtcgtt gttgattcga
    28801 tgaagcgaaa cgagaatgat aaaatccata tccaggccac gatcatcgtc gaaagagata
    28861 gccaaaaagg aatcattatc ggcaaaggcg gaaaaatgct gaagcaagta ggaacgaaag
    28921 caagaaagga tatcgaaaat cttttaggag acaaagtatt tctagaactt tgggtcaaag
    28981 tccaaaaaga ctggcgagac aaaaaagtct atctgcaaga ctttggctac cgaaaagacg
    29041 aatactgata ttctgaactt cttataggtt aagtatagaa ataaactgga taaaaatccg
    29101 cacgttgaga aaaacggcgg aagatcgaaa cataaataat ctggatcggc agaatctgct
    29161 tttccagatt tattttttag gagggagaat atgagccagc ttgcagaatc gaaaggcttg
    29221 attttatcta gccgtaatta caaagaaaaa gataaattag tcaaaatctt tacggaatct
    29281 tctggcaaaa tgatgtttta tatcaaagga atccatcggc aaaatcagcc attagctccg
    29341 gctttgctcc cctttaccga agcagtctat atcggtaatt ttcgaagtga aggtttgtcg
    29401 tttttaagca gtgtcaaaga cgtccatgcg tttcggaaca tccaagaaga tatctttttg
    29461 agcggttatg gtacctattt gctaaatcta gtagatgcag caatcgaaga tcatcaatat
    29521 gacccgcatt tattccaatt tacgcaacaa gctctgcagc ggatggatca aggagatgat
    29581 gcagaaatca tcacgaatat ctttgaagtt caaatactgc aacgcttcgg aatcgcgcca
    29641 atttggacgc actgtgtcgt atgcggggaa acaaaaggaa aatttgacta ttcttccaaa
    29701 tctggcggtg tgatatgcga gaaacactgg ccaatggacg aacaccgtta tcatgcagat
    29761 cctcgcgctg tttatttcgt tcgaatgttt tcggctattt cttatgataa gatcagtgga
    29821 atccacttga aagaagaaac gaagcaggca atccgtgacc tcattgacca gttgtatgat
    29881 gaatatgtcg gtattcatct aaaaagcaaa aagttcattg atcaaatgaa gtcttgggca
    29941 cacgtattaa agacgccaga gaaaaatatg cctgaaaaag agatacctga aaaagaagaa
    30001 taaatgaaag atacaacaaa aaaatcaaaa ctatttgaca aatacaggga cttccgtcta
    30061 ttattaaccg tagattaaat agatcgttca ctgagaggat ctattatata aacaagaaaa
    30121 ctgcaaaatg aatacgtaca ttgaccaaga tcagtaaaaa acacgtggtt tagcgagttc
    30181 gggagagtgg aagccggacc caaacgattt ttgaagaggc acttgcgagt acgataaatg
    30241 aagctgccga atctttattc ggaagtaggg tggaaccgcg aaattattcg tccctatgtc
    30301 tgtaatcaga catagggact ttttgtcgtt ttctttcctg tattcttatt gacagcaacg
    30361 cttaaaaatt gaaaggggca aaacagatga gtaaaaaatt gaccgtccaa gatatgattt
    30421 taacattaca aaaattttgg tcatcaaacg gctgcatgct gatgcaagct tatgacacag
    30481 aaaaaggagc aggtacgatg agcccatata cattcttacg ggcaatcgga ccggaaccat
    30541 ggaatgctgc ttatgtagag ccatcccgcc gaccagctga cgggcgttat ggagaaaacc
    30601 caaaccgttt gtatcagcat catcaatttc aagtcgtaat gaaaccttca cctgaaaata
    30661 tccaagagct ttatttagaa agcttgcgtc ttttaggaat tgatccatta gaacacgata
    30721 ttcgttttgt tgaagacaac tgggagaacc catcgatggg gtgtgccggt gtgggttggg
    30781 aagtatggct tgatgggatg gagatcacac aattcacgta cttccagcaa gtcggaggac
    30841 taccttgtaa accagttact tctgagatca cttatggact agaacgtctt gcttcatata
    30901 ttcaagaagt tgaaagtgtc tatgatctgg aatgggctga tggtgtgaaa tatggcgaga
    30961 tcttcaaaca accagaatat gaacattcca aatattcttt tgaagtaagt gaccaagact
    31021 tgctactaag caactttgac cgttttgaaa aagaagctaa acgctgtatc gatgaaaatc
    31081 ttgttcatcc agcgtatgat tatattttga aatgcagcca tacattcaat ttattagatg
    31141 ctcgcggtgc agtatccgta acagaacgag caggttatct ttcccgtatc cgcaacatgg
    31201 ctcgtgctgt agccaaaatc tttgtggcag aacgcgaaaa attaggattc cctttattga
    31261 ataaaaacac agcaaatcca gaaaacatga aggaggctga ataagatggc aaaagactta
    31321 ttacttgaaa tcgggctgga agaaatgcct gctcatgttg tcacaccaag catgaaacaa
    31381 ttagaagaaa aaacagcaaa atttttagat gaaaaccaat tgacttatga tacgatcgaa
    31441 acgttttcta caccgcgccg cttagcaatc aaagtaacaa acattccaga acgtcaagca
    31501 gacagtgaag aagaagtcaa aggaccagcc aaaaaaattg ctttggacgc agatggaaac
    31561 tggacgaaag cagctgaagg atttgtccgc ggacaaggtc tgacgacaga tgctatcact
    31621 tttagagaat taaaaggcgt ggaatatgtc tatgtaacga aatacactca cggcaaagaa
    31681 gcgaaagaag tattgacagg tttagcagat gtcattaaaa gcctcacttt cccagtaacg
    31741 atgcattggg cagaatacga ttttgaatat atccgcccta ttcactggat cgtggcattg
    31801 ttagatgacg aagtaatccc gtttgagatc ttagatgtca aaacaggcag agcctctcga
    31861 ggacatcgtt tcttaggcga agatgtgaca tttgcgcatg caaaagaata tgaagaaaaa
    31921 ttgaatgaac aattcgttat tgcttcaccg caaaaaagac aagaaatgat cgttacacaa
    31981 gcaaatgaat tagccgaaaa aaatcactgg gttattgatc tagacgaaga tttactagaa
    32041 gaagtcaaca atttagtcga atatccaact gtcttttctg gcgacttcga agaaaaatat
    32101 ctttctgttc ctgaagaagt actagtcact tctatgaaag aacatcaacg ttatttcgag
    32161 gtacggaacg atcaaggtat gctgcttcct cattttattt ctgctcgtaa tggtgatcgg
    32221 gtaaaattgg aaaatgtggt tcgaggcaat caaaaagtat tgacggctcg tttagaagat
    32281 gcggaattct tttataatga agacaagaaa gcttcaatcg accaatacgt tgaaaaacta
    32341 aaaactgtta ctttccatga aaaaatcggt agtatctatg agaaaatgca gcgggtacaa
    32401 gtcattgccc gtatcattgg cgaacaagta ggactatctg aggaagaatt gatggatctg
    32461 caacgagctg ctgaaatcta taaatttgat ctagtaacca acatggtagg ggaatttcct
    32521 gaactacaag ggatcatggg cgaaaaatat gctctgctga aaggcgagaa acctgctgta
    32581 gcaacagcga ttcgtgagca ttacatgcct atatccagtg aaggagagct acctcagtct
    32641 acagtaggtt ctgttttagc gattgcagat aaattagata gcattttttc tttctttgca
    32701 gttgggatga ttccaagcgg gtcaaacgat ccttatgcct tacgtcgcca agcatacggt
    32761 gtcgttcgga tcatcgaatc gaaaaaatgg gcctttcctt tgtctgttct gcaagaaacg
    32821 attagtgatg ttatcagtaa agatacggat cgtttcggca ttggtttatc agaagggcaa
    32881 caacaggtga tcgactttat caaaggccgt ctgcgccaat tattgacaac gaagaatatt
    32941 cgtcatgatg tcatcgaagc tgttttgaat gcagaacaaa aagatctgac aaaagtattt
    33001 gcagcagcac aattgttcaa gcagcatctt gcagatgaag acttcaaacc atcgatggaa
    33061 gctttgacac gtgtcgtgaa cttagcgaaa aaagcggagc ttgaaaaaca atcagaagta
    33121 gaccctgaac tgttcgaaaa tgaagcggag aaagaactgc acaaagcagt agaggcaatg
    33181 aaaaaaagct ttgctgacca aacgatcaac gaaaattatg atcagcttgt aagccttcgc
    33241 ccactgatcg aaaattattt tgatcagacg atggtcatgg cagaagaacc agcagtacgt
    33301 aagaaccgat taacgcaatt aagtcaaatt gctgccatgg ctttgtcgct agcaagttta
    33361 gatcaaatca tcacaaaata atttctaata tgtaaataaa ccagagaata ggcacaaaa
//
LOCUS       DARBDV010000019        32782 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000019 DARBDV010000000
VERSION     DARBDV010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.019"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            45..1186
                     /locus_tag="SCM56_000893"
                     /pseudo
     CDS             45..1186
                     /locus_tag="SCM56_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(1312..2391)
                     /locus_tag="SCM56_000894"
     CDS             complement(1312..2391)
                     /locus_tag="SCM56_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEG1109713.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(2494..2934)
                     /locus_tag="SCM56_000895"
     CDS             complement(2494..2934)
                     /locus_tag="SCM56_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG1109714.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(2991..3401)
                     /locus_tag="SCM56_000896"
     CDS             complement(2991..3401)
                     /locus_tag="SCM56_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEG1109715.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(3581..4015)
                     /locus_tag="SCM56_000897"
     CDS             complement(3581..4015)
                     /locus_tag="SCM56_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG1109716.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(4115..4963)
                     /locus_tag="SCM56_000898"
     CDS             complement(4115..4963)
                     /locus_tag="SCM56_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG1109717.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            5352..7118
                     /locus_tag="SCM56_000899"
     CDS             5352..7118
                     /locus_tag="SCM56_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HEG1109718.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            7220..8479
                     /locus_tag="SCM56_000900"
     CDS             7220..8479
                     /locus_tag="SCM56_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEG1109719.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            8626..9927
                     /locus_tag="SCM56_000901"
     CDS             8626..9927
                     /locus_tag="SCM56_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1109720.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            9930..10781
                     /locus_tag="SCM56_000902"
     CDS             9930..10781
                     /locus_tag="SCM56_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1109721.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            10794..11609
                     /locus_tag="SCM56_000903"
     CDS             10794..11609
                     /locus_tag="SCM56_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HEG1109722.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            11760..12926
                     /locus_tag="SCM56_000904"
     CDS             11760..12926
                     /locus_tag="SCM56_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG1109723.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            13156..13803
                     /locus_tag="SCM56_000905"
     CDS             13156..13803
                     /locus_tag="SCM56_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEG1109724.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            13887..14831
                     /locus_tag="SCM56_000906"
     CDS             13887..14831
                     /locus_tag="SCM56_000906"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEG1109725.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            14966..16417
                     /locus_tag="SCM56_000907"
     CDS             14966..16417
                     /locus_tag="SCM56_000907"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEG1109726.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            16438..17274
                     /locus_tag="SCM56_000908"
     CDS             16438..17274
                     /locus_tag="SCM56_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEG1109727.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            17274..17933
                     /locus_tag="SCM56_000909"
     CDS             17274..17933
                     /locus_tag="SCM56_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEG1109728.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            18059..18748
                     /locus_tag="SCM56_000910"
     CDS             18059..18748
                     /locus_tag="SCM56_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109729.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            18754..19710
                     /locus_tag="SCM56_000911"
     CDS             18754..19710
                     /locus_tag="SCM56_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HEG1109730.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            19754..20266
                     /locus_tag="SCM56_000912"
     CDS             19754..20266
                     /locus_tag="SCM56_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1109731.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(20522..21070)
                     /locus_tag="SCM56_000913"
     CDS             complement(20522..21070)
                     /locus_tag="SCM56_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1109732.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(21159..22340)
                     /locus_tag="SCM56_000914"
     CDS             complement(21159..22340)
                     /locus_tag="SCM56_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEG1109733.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(22576..24084)
                     /locus_tag="SCM56_000915"
     CDS             complement(22576..24084)
                     /locus_tag="SCM56_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEG1109734.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            24243..24434
                     /locus_tag="SCM56_000916"
     CDS             24243..24434
                     /locus_tag="SCM56_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109735.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            24637..25554
                     /locus_tag="SCM56_000917"
     CDS             24637..25554
                     /locus_tag="SCM56_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HEG1109736.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            25547..26620
                     /locus_tag="SCM56_000918"
     CDS             25547..26620
                     /locus_tag="SCM56_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109737.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            26768..27286
                     /locus_tag="SCM56_000919"
     CDS             26768..27286
                     /locus_tag="SCM56_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109738.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            27267..27659
                     /locus_tag="SCM56_000920"
     CDS             27267..27659
                     /locus_tag="SCM56_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEG1109739.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            27735..28670
                     /locus_tag="SCM56_000921"
     CDS             27735..28670
                     /locus_tag="SCM56_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEG1109740.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            28803..29582
                     /locus_tag="SCM56_000922"
     CDS             28803..29582
                     /locus_tag="SCM56_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEG1109741.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            29575..29892
                     /locus_tag="SCM56_000923"
     CDS             29575..29892
                     /locus_tag="SCM56_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEG1109742.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            30136..31746
                     /locus_tag="SCM56_000924"
     CDS             30136..31746
                     /locus_tag="SCM56_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEG1109743.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            31903..32751
                     /locus_tag="SCM56_000925"
     CDS             31903..32751
                     /locus_tag="SCM56_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEG1109744.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        10827 a         5873 c         6398 g         9684 t
ORIGIN      
        1 tatcttattt cttgcataca aaaaaatcaa ggagtgagag agttatgaga tattcaacca
       61 tcttaatatc aacatttgca gccttaagct tatcgcttac tataaatcag acagtccaca
      121 cagaggagac agaaacagcc aaatggacag aaaccaagtg ggccaacgtc aatcgaatta
      181 cctttcagga tgaattttca tccttcaatt caggagtcgc aaaattaaga aaaggacaaa
      241 cagcgaccat caaagcaaaa attgactatt cgggagactt agcacctata ctacaatcag
      301 ctgttacatt tgagaatgtt gatcccactt tgtcgattgg ctatgacgag aacactttga
      361 ctatccaaca caagcaagcg atatttacct tcaccgttac attaaatcag gacttgttaa
      421 aaccagcgct ttttactgtc aaggtatcag atggactacc gaacgatcat catttaacgc
      481 cgtatagtca gcggcagaca attgagcaag attcagccat cgactcagga ggagatcttg
      541 ttgaagaacc aacagataaa ccagaaaatg aaaataagcc agaagtaccg cctacagaaa
      601 atccagacgg agaacagaaa ccggaaatag aacctggaga agaacccgat acagaaactc
      661 aaccagagcc agacaataaa tcaaaacctg aaataacccc tggagaaaaa ccagatgtaa
      721 ctccagaacc agatacggat tctggaaacc aaacagtacc ggaaacaaat ccagacacag
      781 ataatgaaac tgaaaatcca gaaaaaccag aggtagatcc agaagaaaaa ccagatgtga
      841 ctccagaacc agatacggac gcaagagacc aaggaatacc agaaaaaata aacaaaaaaa
      901 caattcaaga ggatggaaaa aaagagagta aaaaatcaaa tctagcaatt ctaaaaataa
      961 atgaagagca gctgaataaa aaatcacgaa tatttgatag cgcccaatca gctgaaacct
     1021 taaaaagtag caaggataca acctttgctg tcctgaaacg aaaaataaac agctaccaaa
     1081 atccggagag tcacaaaaca aagttatact gtggagcgga atcattctat taagtatagc
     1141 aacaatgctt tctgcaaaaa gatttaaaca aaacagatct ctctaacatt tgaataatgt
     1201 ctgtgaacgc atttttcaaa taaaaagaaa tatccagaaa atgaccccaa aataccagat
     1261 tgaaatctga tatttggggt cgttcatact ttatctttct tcatatttat tttaagggat
     1321 atctgtttct tttggaagat ggttcgggat ctttttcact ttcaatacat ctaaaaagaa
     1381 ggtaaagatc ggaatgccga caatcaagcc ccaaacgcca aagaaccgtt cgctaacaag
     1441 taagaccaca aatgtataaa aaatcggcag ttctgttttg ctggacatca acttcggatt
     1501 cagcacataa gactcaatta gatggacaat caaaatcgta atcaggatat aaacaacatc
     1561 tcggatgcct ccttgggaat aagctatgaa agatagagga atacaagaaa tgataacacc
     1621 tgctactggg atcagactta aaatgaaaat catgatcgct aaactcagca gctgatgaaa
     1681 gccaaataca gccaaggcga ttgtagtgag caacgtattt aaaagagcga caacaaattg
     1741 agcttctaat accaaaccaa atgtattgac aaacttatcg gcaaaatagt aaatatcttg
     1801 aaagaaccaa gaaaattcac tttttaaaaa caatcttgag aatacaattg tttttttctt
     1861 ttcaatcata aaaaagaaac ttaagatgaa tgacatagca aacgccactg ccatttttcc
     1921 tatatcctgt aaatagccaa gcgcaatcga agcgccattt tgaagctgtt caaaaaaatc
     1981 gtttcgctca atataagtat ggatatattg taaaacagga tctgcatctt ttggctgatg
     2041 ctcgtaaaaa ttaataacgg actggatcat gtcaaatgtt tgatgaataa gcatcgggat
     2101 atactttgtg aatgccagat agataaggta aacgatcagt gtataaatca tgatagctaa
     2161 aacacctgta gggattcgga caaatcgctg aatgatttga acaagatgaa tcatcaaata
     2221 agtaaagata aatgtgagca aaatggttgt aatcatactt ctaactaaga aaagaacaaa
     2281 aacgacaaat aataaaacag tgagacggcg tattttttca ttcgccaaaa aacgctgata
     2341 aacggtcaaa tcgacaacct ccaaataaca aaaaataaac acgttattca ttatatgatg
     2401 gaatatgaaa ataagaaaga gcaaaaagaa aaaccgctaa atgacgttat cttatgagtt
     2461 ggttttcctc ataaaatcgt tcatctagca gtttcagccc tttttctggt cttgatgaaa
     2521 attatctagc atttttcggg tcagatgttg cagtgtatcc aattcttcct gttcaagtgg
     2581 aagataagac ttgatctttt gtggaatacc ttctgcttgt ttttgcaacg cagcaccttg
     2641 agcagtcagc gtaacattta cgattctttc atcacccgtt tcacgctgac gattaatata
     2701 atttttttct gctaactttt ttagtaaggg agtcagcgta ccagaatcca gacacaaatc
     2761 atttcctagc tctttcactg aagtcttttt cttttcccat aaaactaaca tcacaagata
     2821 ctgggtatag gtaagattca gcggcttgag gaggggagtg tagtgtctaa tgatttcttt
     2881 agaggcggca tataaaggaa aacatacttg attaactaaa tatagatctt ccatttttcc
     2941 acctccgctg ttttactata gtgtatcata gcggatttac acaaaaaagt ctattcacta
     3001 actgctttca gagtaacttc tatatttcca gctgtagctt ttgaatacgg acacacttta
     3061 tgcgctgcct ctaataattc ttgcgtttct tccagagaaa gaccttcgat agatccttca
     3121 atctctacac ctaagacaaa gctcgtttca ctttctgaat aaagggaaac ttgtgcactg
     3181 acagtagaag ccccagagat atttttttgc tgtttgatca attctaatgc actgttaaaa
     3241 catgcactga aaccagctgc aaacaattgt tcagggttgg ttgcattttc gacgcgttta
     3301 ccaggagctg caatattcaa ttcgaatgta cgatcgggat tatgcacttc tccagagcgt
     3361 ccgcctgtgt tgatcatttt cgtagaatac atttttttca taaaattacc acctttaatt
     3421 atattttata caattgaatt ttacacaata taattgtgga aagcaaagaa atggattggc
     3481 ataagataaa taagagaaag cacatgaaac agacgcgctt tctcgaatag caaacatata
     3541 caaaaaatat cagagacaaa aaataatctc atcataaaca ttacatatat ttctcaaaaa
     3601 gttgttttct ttcttcaaaa attacttcac gatggacctg atgaagcatc cataaataac
     3661 caaaaggatc tctgaacatt gagttagtca caccaaagtc catcatttct gtcatttctt
     3721 gaatctctac acagccattt cgtaatgctt tattatgtgt gtcctcgata tcagagacga
     3781 tgatattgaa ccagatcgta tttggatgtt cagcagtagg agcagtcaaa ccaagagacg
     3841 tgttttcatc cagcatatgg aatctcgtac catacaaggt aaaaatgact tcattattgc
     3901 ctctagggta atctgttgcc tcaatcagtt gtatttcatc aaagattgtt tgatacagtt
     3961 ctagtgcttt caaactatca tttaatacaa aatcgatttc cgttccttta tgcataaaga
     4021 tctcctctcc ttttcagtcg aacagttact agattctttt attataccag ctaaagaaaa
     4081 cgcattcaaa aaaatagcgg atttacttac caactcattc gattggatga taagtaaatc
     4141 cgcgtcccat tacttctgct acgctgctga caatgacaaa ggcacttgga tcgatttctt
     4201 ctatcaatgg tttcattcgg gaaaattcat gatcagtcac aacgatcaat aaaacctctt
     4261 ttgactttcg attatacccg ccttttgcct ctagcagggt aaggccacgt ccaatctcag
     4321 cacttaagcg aaggcggatg tcttccaaat aatgttcact catgaccata acagatttct
     4381 ttcgattgat acctgtttcg atataagtca tgacaattga agtgattgca atagataaaa
     4441 tagcataaag aaactcttct attccaaaaa taaacagatt aagagaaaca acgaatgcgt
     4501 ctgtaaaaaa taagccaatc gaagtattca agtggaaata ttttttaaaa atgaggggag
     4561 ggatcgtcgt accgccgcta gaagagttgt ttttatataa aatcgcaact cctaaagcaa
     4621 aaatcgcact tccaaaaatg acagacaaca accgatctga agtaatcatt gtttctggaa
     4681 ctattgcaat gattaaagga aaaaccaagc ttccgaaaag tactttaaaa aagggttttt
     4741 tacctaaaaa tagaagagct agtaccaaca taacgatatt caacactaaa ataactatag
     4801 ctcggtcaaa gcctaaagca gcttccagta atattcctag accgctgaca cctccagcag
     4861 caatatgatg aggccctaaa aacatattga tagcgactcc taatatgaag agggcaaaaa
     4921 taatcttaat gtatgtccat ataaattgtc tcgtttcttt catttattca cttccttaat
     4981 tcattaataa tactcttatc ttattataac ttacttttca ccttaagtta actttaatta
     5041 ataaaaaaat taggctagtc taaattatta tgtttcattc ttctagaata tacttctacg
     5101 ccaaaaactg ttaaaaaact tttactttag aaaattggag aaattgatcg cttaagataa
     5161 attcaggaca ctaaatagta caggaagtag actaaaactt ttaaaagttt taagtctatc
     5221 tatttggcaa gtttaaaaaa taaaaattcg gtatcaaaaa actgttaccg ataacatttt
     5281 tgaaagcgtt gtcttttgct tctctatcac ttatagtgat gacgtcatat taaaacacaa
     5341 ggaggaaatc aatggataca gctgcaattt atcatcgacc ggaaagtgaa tatgcatttc
     5401 tatatacgaa tacaagattt cgtattcggc ttcgtactag gaaaaatgat atcaaaaaag
     5461 tatatgtgct atgtggagat ccatatacca taacaactga aaaatggtat caaaaacaac
     5521 gaccaatgaa aaaatgtcta agtaccaatg ttcatgatta ttgggaaatt gaagtgacga
     5581 gcgaaacaag tcgcttacag tatgcgttcc atgttgttgg ggaagataac acggactgtt
     5641 tttacggtga tcaaggaatt tttccttatc gagaaaatgt gattactgaa ccaaattttt
     5701 attttcgaat accttatttt catcaaatcg atcgattcac tattccagaa tgggtaaaag
     5761 aaacagtatg gtatcaaatt tttcccgaaa gatttgcaaa cggggacaaa gcaaatgacc
     5821 ccgaacacac tttaccttgg ggaagtaaag atccagaccg agaagacttt ttcgggggag
     5881 atttgcaagg tgtgatggat catttggatt acttgacgga ccttggcatc aatggcatct
     5941 atttttgtcc catatttaaa gccacatcca atcataaata cgatacgatt gattattatg
     6001 aggtggatcc cgcttttggc gataaaaaac ttttaaaaaa cttgattgat gaagcacata
     6061 aaagagggat acgtatcatg ttcgatgctg tattcaatca tatgggtgtc cattcaccac
     6121 agtggcaaga cgttctcaaa aacggagaaa aatcgatcta taaagactgg ttccacatcc
     6181 gttttttccc agtagattct tatcagatga ctgacttacc agaaactgct gaaaatatcc
     6241 catatgatac ttttgctttt actccattca tgccgaaact aaatacagca aatccagaag
     6301 tgcaaaaata tttgctggat atcgcaactt attggataaa agaattcgat attgatggat
     6361 ggcgtcttga tgttgctaat gaagtcgatc atcatttttg gaaaaaattc agagaagcag
     6421 ttacagaaat caaaccagac atctatatcc ttggagagat ttggcattcc tctcaagctt
     6481 ggctgcaagg ggatgaattc catgcagtga tgaattatgc atttacagat tcgattaaag
     6541 attattttgc aaaaaagaaa atcacagctt cacaaatggt cagtggaatg aatcatcagc
     6601 aaatgcttta cagagatcag gtgaacgaag gaacattcaa cttattagat tctcatgata
     6661 ctgccaggat cttgaccctt tgccaaggta acaaagaatt gatgaagtct gtgatggcct
     6721 ttatgtttct tcaaaaaggg tctccttgta tctattacgg gacagaaata gggatgactg
     6781 gtgaagatga cccggattgc cgcaaatgta tgatatggga gaaggaggaa caagatctag
     6841 agctattcgg ttttataaaa gaacttgttt ctctaaggaa acagttgtct aagattattt
     6901 ctgaaggttc tactcagtgg ctaatagtaa atgatcgtga agataagctc tatttcacac
     6961 ggaaactaga gggacagatc atctatgttt actttaatca gtcaaaagaa ccttgggtag
     7021 tagaacagga aaatgaggtg attcttagtc aaaattgtca gttgttagaa gacgggaaag
     7081 cagagatacg acaatacgga tttttgatca tgtgttgaaa actgttaccg gtaacaacaa
     7141 gaaagcgttt gcaaatgaag atttgtgccc attataataa gggtgtaaca gcacgttgaa
     7201 tacaaaggag gaaatgaaga tgaaaaaaag aagtgtgaaa cagttgggtt tagggttaat
     7261 ctctttagga atcttaggcg gattagcggc ttgtggcaat ggtaactctt cagcagaagg
     7321 ggaagccgat gaaggaaaaa cgttaacagt ttccgttgat tctggatata aagattatat
     7381 aaacgaaata aaaaacactt tcgaaaaaga aaacgatgtg aaaatcaagc tagtagaaaa
     7441 ggacatgttc gatcagttgg aatcattagc gttagatggg cctgctggaa agggacctga
     7501 cgttatgatg gcagcatatg accggatcgg tgcgttagga cagcaaggac atctggcgga
     7561 agtaaaactg ggaaatgaag cagcttacga tgaaacagac aaagcccaag tgacttatga
     7621 tggaaaaatc tacggagaac ctgcagtaat cgagaccctt gttttgtact ataacaaaga
     7681 tttagtcgat acagcaccat caacgtttaa agatttggag aatctatcca aagacagtcg
     7741 ttttgctttt gagtcagaag caggaaaaaa tacaggattt ttagctaaat ggactgactt
     7801 ttattattcc tatggtttaa ttgcaggtta cggtggctat gtatttggtg atgatggcac
     7861 aaatccttca gatatcggtt tgaataatgc aggtgctgta gaaggaatct cttatgcaac
     7921 agattggttc aaaaatgttt ggccaaaggg aatgcaagac ataaaaagtg caggagactt
     7981 cgccagccag caattcatgt ccaataaaac tgcagcaatc attgacggtc catggcaagc
     8041 gcaaacatac aaagaaaaca atatcaatta tggtgtagcc aaaattccta ctctaaataa
     8101 tggacagcct tatcaaccgt ttggcggagg aaaaggatgg gtagtcagca attatgctaa
     8161 aaacaaagac ctctctcaaa aatggcttga ttatgtaacg aatcaggaaa atcaagaaaa
     8221 attctttgaa atggtaaatg aaatccctgc aaaccaacaa gctagggaaa cagcaaaggc
     8281 taaaaacgat gaattaacaa cagctgtgat tgaacaatat gaaacagctc aagcgatgcc
     8341 aaacatccca gaaatgggcg aagtttggac aggtgcagaa aacttgatgt tcgatgcagc
     8401 atcaggttca aaaacaccaa aagaatccgc agatgaagca gtaaaaacaa tcagtgaagc
     8461 catagaacaa aaatactgat taaaatgata accgtccatt tttataaaaa atggaacagg
     8521 tgcttcagat aaaagtggca agagcgagat ggaaaattat cttgctcttg ttgccattat
     8581 cttaaaagca gtttgcggtt tagcaagttt agagggggca ttcagatgca gcaagataaa
     8641 acaagcaata tcaacgaaaa aaaaatcacg ctttgttcac ttattccagg gatggggcag
     8701 tttgtaaatg gccaagcttt taaaggtatc gtttttttcg ccatattcgt cctattcatt
     8761 ggtcagatgc ttctgggcgg atttcatgca ttggtaggat tggttacttt gggaagtata
     8821 ccaatggaag atcattcatt atttattttg attgaaggca ctttgcaatt catcattaca
     8881 atcattttct tgatttttta cggtttgaat cttttagacg cttatcgagt agcgaagatg
     8941 cgtcaagagg gcaaaaaaat caatcggacg atgaaggaag tcatacataa tataggtgat
     9001 gaaggttttc cttatctatt gacacttccc gcttatcttc tcatgatttt tacgatcatt
     9061 ttcccagtcc tagttacatt gttcactgca tttactaatt atgacttcaa tcatattcca
     9121 ccagccagtt taattgattg ggtaggggct aaaaactttt ttagtatctt ctttttaagc
     9181 tcatatcgta atactttttt tgcagtcttt tcgtggaccg tgatttggac tttatgtgca
     9241 acaactttgc aaatcaccct tggtattctc acagcggttg ttgctcacca gaaatttatt
     9301 aaattcaaac gtattttcgg tatcattttc ttattaccat gggcggtccc agcttttatt
     9361 actatcatga gtttttcgaa tatattcaac gatagcgtag gtgccatcaa tactcaagtg
     9421 attcctttca taaaccagct tccttttgta gatatcagtg caatctcttg gaaaacggac
     9481 ccgaactgga cgaaagtagc aattatctta gtgcagggct ggttaggatt tccctatatc
     9541 tatgtgatgg ttacaggaat cttacaatcg atttcagaag acttatatga agcagcaaaa
     9601 atcgacggag caaatgcatg gcagcgcttt tcaaacatca cgcttcctgc tgtcttccta
     9661 gtggcagcgc caacatttat tacacagtat acggggaatt tcaataattt ctccatgatc
     9721 tatctgttta acaatggcgg tcccggaagc gtaggcggag gagctggttc tactgatatt
     9781 cttatttcct ggatctataa acttacgact ggaacttctc cgcagtattc tatggcttca
     9841 gcaatcacgt tgattatctc cgtcatcgtt atctgtgtgt cattgctggt atttaaaaag
     9901 acaaatgcgt tcaatatggg ggattaacaa tgaaaaaatt tcattcgagt gctcgattcc
     9961 aaaaacgact ttctcacttt tttacttact tttttctttt aatactgtca gtcattatca
    10021 tctatccgct tttgatcaca gcaagtactg ccttcaagtc aggaaatatt gcagctttta
    10081 gtttagatct ttcaagtgac tggactttag gaaactttac tcggctattc agtgaaacac
    10141 tttacgggac ttggtataaa aatacattga tcatttctgt cctaacaatg atcgtacaag
    10201 tgactgtagt aactttggcg ggctatactt atagtcgcta tcgttttatc ggccgcaaat
    10261 caagtttgat gttcttttta gtgattcaga tggtaccgac tatggcagca ttgactgcct
    10321 tttatgtcat ggctttctta ttagaagcgc tcgataagta ctggttcttg acaatgatct
    10381 atataggcgg tggaattcca atgaatactt ggctgatgaa aggatacttt gacactgtac
    10441 caatagattt agatgaatct gctaaacttg atggggcagg gcatttcagg atttttttcc
    10501 agattgtatt accgcttgtc aaaccgatgc tcgccgttca agcattatgg gcttttatgg
    10561 gaccttttgg ggatttcatg ttagctaagt tcctgcttcg aacacctgaa aatcagactg
    10621 ttgctgtcgg cttacaaaca tttatttctg atgtgacaaa ccaaagagtc tctctttttg
    10681 ctgctggggc aattttgatt gcactaccga tttgtctatt attcttcttt ttacaaaaga
    10741 actttgttac cggcctgaca tctggcggaa caaaaggata ggagcagagt atcatgcaaa
    10801 catttccgtt aaactatttt tccgcattac gtacgccaac tcaagtgttt gcgggacgca
    10861 aactattatc atggcctaaa tttttcttga tatttgtatt tttagtttcc ttgatggtca
    10921 tgcctgtgac tcttttttat gccaatcaga tccaagcgat tccattagaa caatttctat
    10981 ccgttcattc gctgatcgat gaacaaggaa cccaaaaatt ttctgaactt gaattatctg
    11041 aaactgggtt acaagcatcg caacaaacga tagctgtgac ccctgaaatt ttggtaggtg
    11101 tgtctttatc tgagaaacaa caatccgatc atggaacttt tattgatttt gagaaggaac
    11161 aatgggtgat ccaacaaaag gataaatctg gtatacgccg ctatacgatg aattatagcc
    11221 catctttcca accagattct gtacgtacgc ccgaagattt ccaacgattt ttagaaagag
    11281 aattttatgc cagtaatcgt ccaacaatca ttttatctta tagtttaagt cttggtcttg
    11341 tcctatttgt catgacgagt ctcattttat tcggggcttc ctttttcttg tggatgacaa
    11401 gaaaaagcca actttcatcc attcatacat tcaaagagag tgcaaatttg atgctgaatg
    11461 ttatgggaat aggttccatg gcagcagcag ttgtagggtt tatccacttt gactttatct
    11521 tgatgctaag tgtccaaaca acgataactg tcttgctgct tttatgggtt tttgtcaaaa
    11581 caagattcaa agataagcgt gtcgaatgac aagtttaagc gatttatgcg acaatgaatg
    11641 tgaaaaaaat gaaaaataaa gtagtttttg aaaaatttct gaaaaaatta aacttcttag
    11701 ttgattcttt tcttttttag tattaacgta atagggtagt ttttttaagg aggaaaatca
    11761 tgttagagaa aactttatac aatcaattat tttcacgttc tttctcgctc cctgtcgaag
    11821 taaattattg ggacggtaca acgaaacaat acggagatac ggacactcct ccccaaatca
    11881 aaatcaatat ccatgaagaa atcccaatga aagagattac aagtaacgca tctttggcac
    11941 taggagaagc ctatatggat catcgaatcg agatcgaggg aagtatccaa gcattgatca
    12001 acgatgctta tcagcaagca aacagctttt tacgaggaaa agattatcta aaatggctgc
    12061 caaagaaaga aaaacatacg aaacaaaaaa acaaagaaga tatccatgcg cattatgact
    12121 taggtaacga cttttataaa ctttggctag atccaacttt gacttattct tgtgcttatt
    12181 tcaatacacc tgaagatacg ttggaacaag cacaaatcaa taaagtccat catatcttag
    12241 acaaactctt tatcaaagaa ggcgaaacgt tgcttgatat cggttgtggc tggggaacat
    12301 tgatgttcac tgctaccaaa gagtataatg tcaaggcaac tgggatcaca ctaagtgaag
    12361 aacaatacga tttgattacc aaaagaataa aagaagaaca tttagaagat aaatgccgtg
    12421 tattattaat ggattaccgc gaactaaaag gcgaaacgtt tgatcatatc acaagtgtag
    12481 gtatgttcga acatgtaggt tctgaaaatc ttgaaggtta tttcaaagtg gtaaaagatt
    12541 tattgaaacc aaaaggaaca gctttgatcc atggaatcag ccgacaacaa ggcggtgcga
    12601 caaatgcttg gatcaataaa tatattttcc ctggcggtta tgtccctggt gttactgaat
    12661 tagttgataa tattacaaaa aacgatttgc aactgattga tttggaaagc ttacgtcgtg
    12721 actaccaatt gacattagaa cattggacaa agaattttca tcaagtccaa gatcaagtca
    12781 cgaaagaaat gggtgattcc ttctatcgaa tgtgggatct atatttacaa gcttgtgcag
    12841 cttcatttga atcaagtaat atcgatgtga ttcaatattt gcttgttcaa cctggaaaca
    12901 atgacatccc aatgcatcgt gcataattga ggcaaaagta ctgtttaaag actatttaaa
    12961 aagaagctaa gttttatgat gctccagaag tgaacttttt tgtctaactt ctaggattgt
    13021 ctctaaagct tagtttcttt ttctttatat tttcataata aaagtttggt tctttaattt
    13081 ttaaacatga cagaaaattt tttcttcgtt atacttgtaa tagaatacta ttttataaca
    13141 aaggggatcg aatcaatgaa aaaaactgtt atttatacta gtttatttgc agctttgttt
    13201 ttagctgctt gtcaatcaaa taccaatcaa ggcacatctg ggaatacgtc ttccagtagc
    13261 gaacagcgct cttcacaggt atcggactcc tcaacaaaag caagcacatc ttcgtctagc
    13321 cagacaaaaa gttctagtga agcaagtact cagtcagata tcaaagtgtc tgcagctcgt
    13381 gcgatcgaat tgttccaaga taaataccca gatactgcaa ttactagttt ggaattagat
    13441 agtgattggg gatcttactt ctacaagata gaaggtgtcg atgatcaaaa agaatacgaa
    13501 gcaagaattg atgctcaaaa tgaaaaaatg gagacagaga atccagaaac tctagatcga
    13561 gatgaacaaa atggtcaaaa gaaaaaagaa gatggtctgg atgttgataa attgatcacg
    13621 attgaggaag cagcaaaaat cgccgtcgac aaagtaggaa gcggtacggc aacagattgg
    13681 gatttggata aagatttagg tactacttat tgggaagtca aagtaaaaga cggcaatcaa
    13741 agtacatcag tcaagatcaa tagccaaaca ggcgaagtac tgtcaactga acaagatgat
    13801 taagaatttc ttcggaaaac aagagttgat ttttctttta gtcatgatac actagacaaa
    13861 ataacttgtt tgaaggagcg atttacttga gtttagaaac gacctttgcc aatcatatct
    13921 ttgccaatcc tttgatgaat gcatctggtg tccactgcat gactacccag gaattagatg
    13981 aactggctca ttcagaagca ggagcattta tcactaagag ctgtacgatc aatgaacgaa
    14041 aaggaaatcc cgaaccacgc tattttgatg ttcctttagg gagtatcaat tctatgggac
    14101 ttccaaactt aggtttttct tattatctag agtatgctct tgcgtatgaa aaagtgcagg
    14161 aaaaccaaaa tcaaccgtta tttttttcca ttgccgggat gagtgtccag gaaaacttgg
    14221 aaatgcttga gaaaatagaa aaaagtgggt ttaatgggat cactgaatta aatttatctt
    14281 gtcccaatgt gcctggaaaa ccacaactag cttacgattt tgaggccact tatgaaacac
    14341 tgaaagaagt attcagcatc ttttctaagc cgctaggaat caaattgcct ccttattttg
    14401 attttgctca ttttgatcag atggctgata tcttgaatca gtttccattg acctatgtca
    14461 atgctatcaa cagtgtgggc aatggtttgt atatcgatac agaacaagaa gcagtagtca
    14521 tcaaaccaaa agaagggttc ggcggcattg gcggagagta tatcaagccg actgctttgg
    14581 cgaatgttcg cgctttttat acacgtttga aaccagaaat ccaaatcatt ggtacaggtg
    14641 ggattcggac aggacaagat gcatttgaac atctgctatg tggcgcaagc atgttacaga
    14701 ttggtaccga gcttcataaa gaagggccgg aaattttttc tagaatcata aaagaattaa
    14761 cgcagattat gtcagaaaaa ggatatacgt caattgacga atttaaaggc aaattacgca
    14821 ccatttcata gcaaagagac gaaggtcatg attgatcttc gtcttttttt ataaaaaaca
    14881 ctcgtctgtt cattcctgtg caagcgtact cattgtatgt tagacgaact tgtgggaaaa
    14941 taacagaggc aaaaaggagg ataatatgaa tgatgcaaca gaattagcaa aagagcttaa
    15001 agaaaaagaa tttagttttc aagaactagt tacggatata cagaaaaaaa taaagaagca
    15061 aaatcatgaa ttgaatgcgt ttgtcgcgct tgaaccattc gagagtaatt cccacgagct
    15121 attgattccc cagtcagaaa aagggccatt ggctggtatc ccatttcctt taaaaatgct
    15181 cggtcaagaa aaaaagggat ggctagctac ttcaggatct cgtttatttg aaacacaccg
    15241 agcttctcat acatctaatt atgtgcggca agcagaagcg ataggtctag ttccttttgg
    15301 acaaaccaat gcgccagagt ttggtttcaa aaatattaca gacccagtca tctatggccc
    15361 agcaagaaac ccttggaacc ttgaatgtac accgggcgga tcaagtggag gagctgctgc
    15421 tgcagtttca tcaggaatcg ttcctttagc tggtgctagt gatggcggtg gttctattag
    15481 aatacccgct tcgttttgcg gattgattgg gctgaaacct tcgcgaggga cgatgccagt
    15541 tggtccatat gcttggagag gatggcaagg agctgctatt gatttcggct tgacagtttc
    15601 tatgagagac acggaggctt tattttatgg gatgagaagt atccattccg gtgcgccata
    15661 tcaagtatcg ccagttgagt ggcaaacaca tccaagaaaa aaacggttga agatagcttt
    15721 ttgtgttgat tcaccgatcc actcacagat tagtgaagag gcagtacttg ctgtaaaaaa
    15781 agctgtagat ttccttggaa aagaagggca tgatttgaca gagattcctt acccactaga
    15841 tggtcgtaaa ctgatcacgt cttattatca gatgaatggc gcagaaacag cagcaatgat
    15901 ccgctcgatt gaaacaggca tccagcgagc tgtcagaaaa gaagaacttg aacccatttc
    15961 ttggactttg ttccaatatg gtcagaagct ctctgccgct acttacatcg attctctgca
    16021 tgtatgggat gaagcagcaa ttgtcatgga aaacctacat caagaatacg atctattcct
    16081 ttcaccaacg actgcattta ccgcccctaa aatcagcgaa gatttgcaaa gtgatgcgat
    16141 aagagcacag atggaacaca tcagtgattg ttctgaagta gaaggtttag acgttgtttc
    16201 ttccatgttt gaggagagct tgaagatcac accttataca cagttggcta atctgactgg
    16261 acaaccagca atcagcttgc ctactcattt gacaaaagaa aacatgcctc taggaatcca
    16321 atttatggct gccagaggaa gagaagattt gctatttcaa atcggctatc tatttgaagc
    16381 aaatcatcag ttccatttac caaatccatg gaaatagcag aaacaaggag aagaagaatg
    16441 aagaaaaaaa gattgtttgc tctaataact agtgcctttt ttattttaag cgcttgcggt
    16501 acagctgccg ctaatcagga agataacagc tggagtaatg tagaaaaaaa gaaagaatta
    16561 gtagtagcga cttctggcac actgtttccc gcatcttatt ataatgatca aaatgaatta
    16621 gttggttatg atgtagattt ggcaaaagag gtggctaaac ggttaaatgt caaaatctcc
    16681 tttaaagaat acaacgtgga tggacaaatc acttctgttt caaaaagaga atcagatttt
    16741 gctgcgaacg attttggtat cacaaaagaa agagaaaaaa aattctccct ttcagagact
    16801 ttcaaatatt catttgacag tatgatcgta cgaaaaaaag atgattcagg aatccactca
    16861 cttgaagatc ttaaagggaa aaaagcagct ggcgaaccca atactagcta tatgcgttta
    16921 gcggaaaaac acggagctaa attagtcaca tatgacaatg ccacgaatga tcagtattta
    16981 agcgatgtag caaatggtcg aacagatgtt attctaaatg attattatct gcagaaaatg
    17041 gccgtatctg ctttaccaga tatccctgta aaaatactgg agaacgttta tttcaatgaa
    17101 aacgaaagtg gttttttatt gaaagaagga aatgatgctc tgaaaaagaa aatcgatggc
    17161 gtattaaaag agatgaaaga agatgggacg acaaagaaaa tatctgaaaa atattttcaa
    17221 actgatgttt cagtcaaacc gaaagaaacg atggctgagt cggtgagtga gtgatgtact
    17281 ttcccctaat tgatttttcc ctcatgtgga cttcacttcc tttcgtactg caaggcttgg
    17341 tatacacatt agggattggc ttcgtgtcgt ttcttttagg aaatttggta gggttgcttt
    17401 tgacagtttt aggtcttttg gattggctgc ctctgaatgt atttattcgt ttttatttat
    17461 cgttctttag aggcattccc gcactagtcc tgctgtttct gttatatttc gggttacctt
    17521 atcaattaag tgccttaacg gcttcagtca tctgctttac gattaccagc agtgcattta
    17581 ttggtgaaat ctatcgagga tcactagcag gcgtttcttc aggtcagtgg gacgctggtt
    17641 tagcttctgg tttttctttc tttggggtat tgaaatatat cgttttgccg caagcttttc
    17701 gaatctcagt accggcactc agtaacgtag ctatggatct agtgaaaggt acttcgttag
    17761 cagcaacaat cacagtgcca gaaatatttc aaaaagcaaa gatcataggc ggtcggacat
    17821 tcgattattt ttctctctat attttagttg cactgattta ttgggtcatt tgtatcggga
    17881 tcgaaaaagt gcaacagtat ttagaaaaac gttctatcca tcagtcaatc tgaaaaatag
    17941 aaatggtgct agcgtggctg gcaccatttc tattttttgt ttcttgtata ttctgcctag
    18001 tattttaaaa ccatttcatg taaaatgtaa atcgaaaata tgttcggagg tagaaaacat
    18061 gaagaccccg ctaaccatcg aaatagaatc ctcgttatac caatattgca tcgagcaagg
    18121 cggcttggtt gtagaagaag tcacgatgcc tgatgaccaa ggaattgtag atacgcttag
    18181 ctgcttgaca aaatttgatg gaagccgaga atggcgatgc tatgagctga aagtgacaaa
    18241 agcagatttt cgctctacag ccaaactgtc atttgttgga cattataatt attttgtttt
    18301 accaagtaaa ctatatgaag aagtaaaaac tgaaatccct acaggaatag gtgttttagt
    18361 gtatcgtccg tacacggttg aagaggagat gcctgcctct ggtacatttc tgattgcgaa
    18421 aaaagcagca aagaaagagc ttgctgtacc ggaagaagct ttgacaaacc gcttcatggc
    18481 ttctttattc agggaagtaa gaaaagcaaa gcaaatggag cgaggcgtaa aatattttcc
    18541 aaaagatcaa ttgtacaaag aattgaaaag acgttcagaa aatcaagatc cattttctaa
    18601 cgaacgatat tatgaacggt ttttagagga aatagctagt caggcaatca atgagttgca
    18661 agaagaagtt atcgcgcttc agcatgatta tgaatatttg aggcaacaac agcagatccg
    18721 tcggttgcct acagagccat tggagtgaaa aatatgtact atccttattt aagaggaaaa
    18781 caatttgacc tattagcatt aaaagaagca ctgtcgcgag gattgctctc caataaaatc
    18841 caaccagtga tcgaaccagt acgtgattcc gctacgctta aaaatgtgat cgaattattt
    18901 caaaaaaagc accatccaat cgctgtgatc caaaatcccc aagtaggaca attcaaatta
    18961 ttcgatcagc atgtgcacag ttggacagtc aaagaaaata gctctgtcgt ccctgcacag
    19021 atcttaacgc cagaaaatct cagtgaagta ctggattcac ctccagcgtt tcttgttttc
    19081 gatggacagc actacccaaa agatacagag gtgtggaaac agttggcagg agttgattcg
    19141 aaatttttga taccagatac tagtcgcttt cgcatctggc tgcccgaaaa caaaattgtg
    19201 atccgtgact cttttcaaac gagaaagcat gttgaaagtt atgcagataa aacagatgac
    19261 ttttttagtg atgactatct ttttttccac gcagacggat atattggatt ttctgatttc
    19321 acgatcgaag gtagccgtta tttcgataaa gggttcccta gccgagcgct tgctatccat
    19381 ctaacgtata ttgatgctta cggcaatata cgcgtgaaac attttgtctc tgacagtaac
    19441 gactctgcta aagaccaagc attgaagttt cttgaagcag gagataaaat gaaaaaatgg
    19501 atcatgcgtc accatcatca actcttgatt acttcaggaa tgattgaatt attggctctt
    19561 taccagcaac aaaaattccc tggactggga gtgttgaaaa agtggtcact tatgcatcat
    19621 ttggaactta ttagtcacct gctagatcat ccaaaagatt ggcttaaata ttattcaaaa
    19681 gttcaggaac tatatgaagt gatccaatga ttagataatg taaatgaaaa aggaatagaa
    19741 aagaggaaac tggatgtata ttcggcaaac aaggaaagaa gaagtagaag aagtggtaga
    19801 aatcattaat gccgcagcgg atttcattgc ttcgcaaggc tctcctcaat ggcaaaacgg
    19861 gcagaaacca acaaaagaaa cgattttgaa cgatattgaa agaaaagaaa gctatgtatt
    19921 aatagtggaa gatcaaattg ctggaacagc tgcattagtc tcaggcgtgg atcccgttta
    19981 cacaaaaata gatggtagtt ggaaagaatc aactggtcct tatttatcga ttcatcgtgt
    20041 cgctttagca gaaagtactc gaggaaaaaa actagcaaaa ccgttcttag aacatttgtt
    20101 gacgattgca gccagcaaaa atatccaaga tatccgtatc gatacttatc cagctaatga
    20161 accgatggaa aagaccatct attctgctgg attcacttat caagggatga ttgaatttcc
    20221 ttttgaacac ggcgaaagaa aagcatatca aataatcatc aattgactat aaaatcatct
    20281 gagcaaagat aaagacatta acactactga aaaaaacaat catatgatag ccaagaggtt
    20341 gtaacagaag tggccagctt caaaaaacaa gaaagaattt ccgaaaatgc ttctcaaatt
    20401 tttgtggaat tttagcgtat tttacgaaat agctgctttc tgaaactccc ttcatcgaga
    20461 ggtcgtgaca tagcttttgt cacgatctct cggcttatcc tacgagtatt attcgataca
    20521 tttatatcaa taatcctttt tttattttat ctgcagcttc ttggaacgct ccatcgtttg
    20581 acacgactaa aagtgagggg atagctttca aacgtttcaa ctgtgctaaa tcttctacat
    20641 aaaccgccag tctggctgaa atctgttcag atgaatctcc acgttttttc aggcgcgatt
    20701 gcaatgtttc tttagagatc gtaataaaga taggacggac taccgtcccg aaatactcat
    20761 ataaatgcca aaaaccattt gctgtcaaaa cggcgtaaca atttcctact gtcagtttct
    20821 ttttaatttc tgctttactg gaaccatatt tatgttgagc atattggtcc cactctgcaa
    20881 actcatttcc cttgatcatc tgattgaatg tattgtcaga aacaaaataa taatcctctt
    20941 gatctttttc cccaggtctt ggtgatcgat tcgtgtaagt aatgatcttt tgttccggct
    21001 gaaaaatagc ttctgcgagt gaagtttttc cagaaccaga aggaccgata aatacatagc
    21061 aataattcat tgtcatttct cctttgatga ttgaacaagt gaaaaagctt tggaaaatat
    21121 ttccaaagct tttaaaagta actttgcttt tttgtatttt ataccagcaa gccagtcaca
    21181 atagcactta aaaagctgac taaagtcgct gttagcaaaa ttttcaatcc gtgttttgcg
    21241 acaagatttc ctttttcttc atttaatccc ttcatcgcac cagaaatgat cccgatagaa
    21301 gagaagttgg caaatgaaac gaggaagaca gatataattg ctgttgtacg ctcagtaaaa
    21361 tggaaattac catttgctaa ttctgtcata gcaacaaatt catttgtaac cagtttagtc
    21421 gccatgatac taccggcatc cactgcttct ttccatggga ttccgctgat gaaggcaaga
    21481 ggggcaaaga tgtaacctag gatctcttgg aaagtgatgc cgaatatccc gtggaagata
    21541 gcattgatca tggcaatcaa agcaacaaaa ccgataagca tagcagcaac cgtgatcgcc
    21601 acatggaaac catcaagaat gtattctccc aacatttgga aaaatgtctg ttctttgttt
    21661 tcttcaatga tcagctcatc ttctttttct tctaatacat agggatttac aattgaagcc
    21721 agaatataac cgccaaataa gttcaaaaca agggcagtga tcacatattt agaatcgatc
    21781 agtgccatat aagatccgac gatcgacata gaaactgtag acatagccga tacactcatg
    21841 gtgaacaaac gtttctcaga taggaagggt aattcctttt ttatggaaat aaaaacttcc
    21901 gattgtccta aaatagcaga agctactcca ttataagatt ccaatttgcc catcccgttg
    21961 attttactta atcctagacc aacagctttc ataaataatg gaagaatctt gatatagcgt
    22021 agaataccga taatagcaga aataaataca attggcatca atacggaaag aaagaaaggt
    22081 gcacttcctt tatttgctac accggcaaaa acaaagtcaa caccttgccc agcaaaagca
    22141 agaagatgat caaacatttt tgccaataca gaaacaaccg ctgtaccaaa agttgtacgt
    22201 aataatacga aaccaagaac aagctgtaaa acgaacatca caattagtgg tttcaatttg
    22261 attgcttttt tgttactgct gatcaaaaaa gcaagcccta atgtgacaaa taaaccaata
    22321 atacctaata aataactcaa ataatcactc ctattccaaa ttccgtttta ttttacctgt
    22381 tttgcaaata aaatactaat ctttagtgaa attttcatta cttttttaga gtgaaacgca
    22441 ttcattttcc attagaaaat aaagcgaaca aaataaaagg tttaaaaagt gtaacgtctt
    22501 agtaaagtct ataaaaaaac gaaactatcg attaagctaa tagtttcgtt ttcaacattt
    22561 ataagtaaac tttttctatt ggtaatatag attttcggaa gggtagacag gtgcgcaggt
    22621 aatatcgatt cctagttttt tcaatgtttg ttcatcattt ttattgacca tcacagtaga
    22681 gtgggcttgt gctccctcaa gttcagctag tttatcataa gcaacttgag cgattggatt
    22741 cgttactgca ctgattgata aagcgatcaa gacttcattg atgcttagtg catccaaaga
    22801 ggaatgaagt gtctttgatt tcaatccttg tattgtttca agaactaatg gtgacaataa
    22861 aggaatgtca tctgcgatat gggctaaatg ttttactgca tttaagataa ctgccgctga
    22921 cgcatccatc aatgaacttg tacgcccggt aatgatgact tcatctggta attcgattgc
    22981 aatcacagca gaaggatctt ttgtaggact ttgcgcttga atcttatctg catagtcatg
    23041 ggcagggact accacgtttc ggtcttcagg acgtaaaccg acttcttcca taatcagttt
    23101 gattcgatta actgcttctt cgtcgatcaa tccttttttg aagtcgcatt ctgtttggaa
    23161 ataacgacgg atgatttctt gtttagaggc ttcttggaca acggtatcat ccacgatccc
    23221 aaatcctaca cgattcacac ccatgtcagt aggagattga taaacagatt ctttaccagt
    23281 aattttttca ataatacgtt tgattacagg aaatgtctca acatcccgat tatagttcac
    23341 agctacttta ttgtaggcat caaagtggaa ggaatcgatc atattgacat cttttagatc
    23401 gactgtggct gcttcataag caatgttcag cggatgtttc aacggaacat tccatactgg
    23461 gaaagtttcg aacttggagt aaccagctgc atgtcctttt tggctttcat gatacagttg
    23521 gttcaagcaa gtagcgagct ttccacttcc tgggccagga gctgtcacta caacgattgg
    23581 tttagttgtt tcaatatact cattttttcc aaaaccatct tctgagacga ttttttctac
    23641 gttgactgga tagtcttcga tttctgagtg tttatagacc ttgatgtttc gtctttctag
    23701 tttattgata aagactttcg tggcaggctg tccactatag cgagtgataa cgacactgtt
    23761 gattaataag ccataaccac gtaattcatc gattaaccgc aagatatcca tatcataagt
    23821 aatcccataa tcaccacgga tcttattacg ttcaatatct ccagcataga cgcaaatcag
    23881 tatttcagct tgatctttca atttttgcag caatttgatt ttcgcatctt cttcaaatcc
    23941 tggaagtaca cgtttcgcat gcttgtcgtc aactaacttc ccaccaaatt ctaaatataa
    24001 tttgtcatag tggttcactc gttccaaaat atacgctgac tgctcttcaa tatatttttc
    24061 tgaatcaaaa ccaatttttt tcactaggtt ccctccttaa gtactaaagc attttttcat
    24121 ttttttgagg attcgtcaac tggaagagcg aaattcctca aaaaaacaaa gaaaatggtt
    24181 taaaccaact tgtctagata gttattcatt ggtatgatag tttttgataa ggaaggtgct
    24241 acatgaaaaa aagacagaaa aagaaaaacg catataaaca ctatatccgt tcgattttta
    24301 ccggttatga aagaatgctt gaagatcccg aattggaaca attgacgttt acttatctaa
    24361 acgaagaaac gcagctaact cgtgatgagc atcagcgcat tcatttcaca acaagagatc
    24421 tgcctagtaa ataaaaaata agataaaggt catcggattc gattctgtcc tatcagatgg
    24481 aaagactagt tgatcgaaaa aaaggctgta ccaaattttg gtgcagcctg ttttatggag
    24541 cattgcccgc tcttttttat aacaattttt tacagaaaat ggtactattg aagtattaat
    24601 aagtgaaaaa cagatgagta agaggaggca agtagtatga aagagccaga tacaaatgca
    24661 aattatgagc cagacgaact agaggagacg aacgcggatc aggatacagg acaagaatcg
    24721 tcttcttttt acgatatcaa agaaaaagca agcatgaatc tttcccaatt attagaagac
    24781 ttacaggctt ttgaaaaagc tgtccgcgaa gaagatatcc ctgagatcta ccgtttatac
    24841 aatggccgat tgaatgaaga attaaaaaga agctcaaatg aaaatcacga gatcgatcaa
    24901 ttgctgatgc gtaagatcca tgatcgtttt ttacaggaat ttccttttat ggaacaagta
    24961 gaaaacatct cgccaacgat gagctattac aaaatcggca cttattacca tgatcggccc
    25021 acggtcggta ttgatgcaag tctgccagaa atttttgttt tgcctaagat cgatgaagag
    25081 tgggagaaat attcccaacc ggagtcaacg gactatgaca aaaaaatcaa tgagctagac
    25141 gcaaaggtga tcactgctca gacagaaatt gaacgtttag atgaacaaat aaaagaaatc
    25201 aatcgacaaa tgactgattt gaacgacaat aaaggattct tgaatcgtaa aaaaatcgag
    25261 gaagaaattc aagaattaga gaagaagaag caagtactat ctaatgaaaa attaggctgg
    25321 cttccttata ttgagacgcc tgaaacaatc cagcagcaaa aagaagcatt gaagcaagaa
    25381 gcaagagcag accagcttcg tgcagccatc gtagaaaaag aacaaaggca aatcagacgc
    25441 tatttcggta gcaaagaggg attcggtcaa gcaatccatg aattcttgat gaattatctt
    25501 ggcaatgaga atcctaataa tcaatctaat gaaggaggtt ccgagtatga atgaacacga
    25561 tgaagcagaa tggacaaaaa atgaagaaca aacagaagat catgaagaaa cgaacacttc
    25621 attggaagaa caaacaagta atcctctgga tgacacagaa gaaatcccta tgctagagga
    25681 aacaaaaata catgaaaaag aaaaagtaac tccgacgaat acgaatactg cagaaaacac
    25741 ccatgcttac gaaataactg aaaatcccgg gtatttagaa ggaaaagagc aagatcatca
    25801 agatatcgtc agtttgattg aaacagcgga agcagaatta gcggatttac gtatgagaaa
    25861 agatttgatc gaatcagatt ttccacaatt attagaatca tatgatcgtt ttatattgaa
    25921 tgaagaacca atctccacga tcggaaagac aagcctgatc gaatacttca cgtatgaatt
    25981 actgaaaccg ttgaatgata ttggtctgga acggtcaaat tcagcttatg acacatggaa
    26041 aacaagacat ttttcgatca attatacgtt gaatggacaa aacgaattag tcttttcctt
    26101 tgtattaccg accatcgatc aacgatacca agtggaatcg atgagtctcc taaacgtatc
    26161 accagagaca atggaaatca atgtccaaga tgaccgggtg ctttctttga tccgacactg
    26221 gtcagtggat cgagtattct ctgcagggca gatcacgata ttcaatcaca aattgaatca
    26281 attattagcc catgcaagaa cacttggttt taccgttaat cagacattac tagacaatac
    26341 gaagccactt cgtttgaatc ttcagagcga atttgaattg acgaatgaag tattggatga
    26401 tatttttatc gtggcaatga aaaatccatc ctacgatatg gaaaagatca acgaaactac
    26461 ttatcaagta cgattagata aaggacagtc tttaacgata acaacaaacg aaaaagatca
    26521 aacagtcttg tcgatctcat ctggagacta cccacgatct gtcattgatt tctttatcaa
    26581 ttacgaattc ttggtcccat taatggtgag aaagtcctga ttggataagt tggattacca
    26641 gatgtcattc agataattct gttccaaagg acttgcggca gttatccaga ataattgtca
    26701 caagtctttt ctgtatgtac taaatggacg tgaataactt gctgatgaag gagggatttc
    26761 attaggaatg tccacaaata aaatagcaca gcttagctta ttgtctgctg cttgtgtagc
    26821 aggtaggatc gcttttcaat ttattccgaa tttccagcca atgactgcta tttttttatt
    26881 tatagtactt tacttaagct taaaagatgc gttaattgtc atgagtcttt ctattgcgat
    26941 cagcagtttt tattttggtg ttggaccatg ggtcatcggc caatggatca gctatactgt
    27001 tgttctgagt ttgttttcaa tcgtctgctt gagaaaaaca gtgcaaagga atctatggtt
    27061 aaagggagtc tgtttttttc tggcgggcat ggtttacgga ataggtatga ccgtttttga
    27121 tacgctgtta tatcgtttgc ctcaaccttg gatctattac cttcaagggg tgtcgtttga
    27181 tttgatgcac agtataggca atgttgtgtt ctttcttgtt tttctgccag tcgtcaaacg
    27241 tttttacaat cattcaggag gaaaaaatga aaaaaataat ttatagtttg cttgcaatga
    27301 gtgtcagtat actcattttg acaagttgca gtacaaccta tacacaatcg gcttccaacc
    27361 aaaaggagga agtaaaaaca tcgcaaaaag caacaatcac gttacaagaa aatggtcaaa
    27421 acttttcgga aaaagaagtg gtcttcaaaa aaggcgatga tttactgagt attttgaaac
    27481 gaaattttga agtcaaagaa gataacggct tcatcacttc gcttgaaggg cactcacagg
    27541 atgagaaaaa tcaactctat tggatgttta ctgtggacgg gaaaagtgct actactggtg
    27601 ccaaagagat caaattgaat gatggacaat cggtcatttt caatttgtct aaactataag
    27661 tttcataaaa aactgttaaa gggcgaatat tgccataatt cagtgagttc agttgatgaa
    27721 agaaggaaaa taacatggaa aattttaaaa aaagcggaat gttttccgta attgctgctc
    27781 taggggctaa tattctagtt gctatcagca aatttgtcgg ctatgcaatc agtggcagtg
    27841 ccgccatgct aaacgaaagc atccacagta tcgttgattg cagcaatcag attttgctgt
    27901 tattcggtga taaacaagcc aataaaggac agagcgagtt gcatcaattt ggtgaaggac
    27961 gagcaaaata ctttttcagt acaatcgtcg caatgatgct tttcttcggc ggtggggcac
    28021 ttggggtgat ggaagctttt gaaaagttga cgcatccatc ccatgaagtg ggtaatccga
    28081 tcattgttat agcaatactg ttatttggtt tggtaatcga aggaagttcg ttacgcgtgg
    28141 caatgaaaga aatcaaagaa ttgaatactg aaaaactttc cacgatgcgc tttttgcgcg
    28201 aaagtcgaca cagtgaaata ctgattattt ttacggaaga cttgtgtgct gtaatcggtt
    28261 tggtcttggc tttagcagga acaatattga cttatgcaac cgggattgct gcatttgatg
    28321 cgatcagcgg attgctgatc ggtttcttgc tgatgggagc cgccattttc ctagcaaaag
    28381 aattttatag tctgatcatc ggtgaaagcg tgacagcaag cgatttatca aaaatcaagt
    28441 tagcttttga acgtcctgag atcagtcggc tgatcgatat aaaaacagtt catttaagtc
    28501 caacggagat tttagttgca gcaaaagcag atatcgttgg taacaaagag aatgaagctt
    28561 actcggtcat caatgatatt gaaaaaaata tgcgtcagtc tcttcctgac aagaagatgt
    28621 atatatacat tgaaacagat aaatatgacc caaattattc tcgtaaataa aacgtttatt
    28681 catgaattga agcaaaaatg agaaaaatat aatcttgttt ttaaataaaa cggctacaat
    28741 ttagcgaatt tatgttacaa tgagtccgct gtttatttgt tgtaattaaa ggagatattt
    28801 taatgaacaa aaagaaaagt gcgccatggc ttcaagcttt gcatgcagtg atgccacttt
    28861 gtataagcta tataccagta ggattagctt gcggagtatt gttgcaaaaa gttggatttg
    28921 atcctctctt atcaggactg ttatcgattc tggtcttttc cggtggcgcg cagtttttag
    28981 ttgcatctat gcttacgacg caagcatcat ttgcaacgac attattgatg gttttctttt
    29041 tagaattacg ctatacatta ttaggagcaa gtcttgccgg ttttatgaaa aaagaaaagc
    29101 ggacgttttt ggctgtcttt tctcaatcac tgaatgatga aaactatgcg gtgaactact
    29161 taaagttttc aactgaccct tcctggaata aaagaaaggc tttatatgtc aattggttct
    29221 caatgactgc atgggccggt agcaatatga tcggtaattt tttcggctcg gtgattcgag
    29281 tagatgctga ccttgtccat tttgctttga cggccatgtt cattttcatg tttatcatgc
    29341 agatgaaaag cgcactactg atctttacag gtttattctc aggcgtatta ggggtcgtat
    29401 tcatggtatt attccaaaat actttcggat tgattgcagc aacagtcatt gcttcatttg
    29461 ccggatttat gctggagaag gcattcaaaa aagaaaaagc taagaaacta agagaccgcg
    29521 gtaaagatgt caatgagccg ctcttcaact tcccggaaca ggaggccaat caccatgact
    29581 aaactatatt tgctactttt agttgcttgt ctgttcatcg tggcatatat cccgcgcttg
    29641 ttcccgatgc tttattttac tcatcgaaaa gtgccatcct ggttcagtga ctggatgaaa
    29701 tatgtaccgg tggcattatt tgcggcgtta gcttttaaag atgttttcat tactcatgaa
    29761 catctggata ttgcttggaa tatcaaaata gctgcaatga ttctagtagc aggcgtggct
    29821 tacaaaacac gttcgatggc gttgtcagtt ttgacaggac ttgcgtctgt ctttttgctg
    29881 tctatgcttt aaactgtttt attttattaa aaacagagat tgcgacataa gttgttcaaa
    29941 ctgatctatc cgaactgcat ttgcaacagt caattctgga tgcaagttta ttcgtaggga
    30001 taaaaaagat tagtgttggc ttatgatgca agctcttttt tattttagga attagagagg
    30061 gatttgtcct tctatgattt tttgctataa tagggaagat gtttatttta tgaaagaata
    30121 gttgagggaa tttaaatgaa taaaaaattg atgcaaggaa cattttggct aacctttgct
    30181 aatcttctat gtaaagtttt aggggtagtc tatttgattc cctggctaag tatgatgggg
    30241 aacaatcaag atggtatgct tgctacgaca ttgtataatg ttggctatct tccttatgga
    30301 ttgttcctga tgcttggtac agtcggattt ccaaatgcga ttgcaaaaaa agtagctgta
    30361 gccacaaaag aaggagataa cgctgcttgt cggctgattt ttagaagtac gatcaatatc
    30421 atgtttgtca tcggtatcgc ctctgctgca ctgatgtatt tatttgctcc tttactagcc
    30481 ggaatcagtc cgattgctaa cgttaataat gggatactag ctatcagaag tttatgccca
    30541 tctttgattg ctatcccaat tcttagtgca atgagaggat atttccaagg aaaaaatgat
    30601 ctgcggcctt atggaacttc tttgattatc gaacaggctg tgcgagtgat cgttatcttg
    30661 gctggtactt attacttgcg cgtattgact gacggaacca tattagaagc tgtattgatc
    30721 agtacagtcg cttctttctt cggtgggtta gctgcgatta tccatatgta tatcgtcgga
    30781 cgcagaaaag acttcttcga acttaaagat tttttgattt ccagccgtta tttcgaaaga
    30841 gaaaaccgat ctgcctctgt ctctatcatt cgggaaacac taccgtttat ttttgtcggc
    30901 tcagtcatta ctatcgttca aatgatcgac caagtaacga tgaaaccact gcttcacttt
    30961 ttccgtccag agatcgcaga tcagcaactg gaattcttat tcagccgtgc ttctgtcaat
    31021 ccaaataaat tgacattgat cctgatctcc atggtaggaa cagtagctat aagcagcttg
    31081 ccaatattaa gcacgatgaa gaaaaaagat cggatacaaa tcgaaaaaac agtaggagat
    31141 agtttttcga ttgcgttatt gattcttctt ccttcattga ctggaatgtc tttattagct
    31201 ggaccactat acacattgtt ttttggctat gatcctgaaa gtgtcggata tttccaaatg
    31261 gctttgctag cttctttgtt tttctcttta ttcacgatcc tatctacgat gcttcaatct
    31321 ttgaatcacc atcgttttgc gatccgatta actgtagaag caattatcct gaaagtcatc
    31381 ttccaagtaa tcggattagg attagttggc ggatacggga tgagcttatc aaatggctta
    31441 gcattcggta ttgtctttgt tcgcggctat cactttatgt gcaaagaata ccgaatagca
    31501 cctttagcaa agatcagcaa ttttttctta aaaactttcc gctccaccct gattatgctt
    31561 gcagtatgtt ttgccgtttt ctttttgctg aatcagcgat tgtcaatgga atcaaaaagc
    31621 tatgcaatga tttattgtgt ggctgtagga agtattggcg gcttagtatt tttattttct
    31681 caattcggaa aaaatgggtt tcgaatgttg aaaaatgtta aacatcatag cagaagcaaa
    31741 gaataggtta actaatcgtt ttttgctgtc acttgaaaaa cggcaactcg tatgattacg
    31801 cttgtcgttt ttctattttc caattttagt ttcatatcta atcttactta tgatatagtt
    31861 ataaggtctg gaaaaattct ttgaaacgag gaaattaaac acatgaaact tgcagtagtt
    31921 acagatagtt cggcatatct gccggaacgt atcaagaacc atcccgactt attcgttatt
    31981 ccaatccctt tgattatgga tgggaaaatt tataacgaag gaatcgatat cgaagcggat
    32041 gaatattatg gacttttaaa aaacagtaaa gattttccta ctacttcaca acctgtcgta
    32101 ggggaagttt tgggtttata tgaggaattg aaagagaaag ggtatgacac gattttaagt
    32161 atccatcttt cttcagggat ttctggcttt gtcaacacat tatttgcgat gaaagatgac
    32221 atcagcggag taacggtcat tccttatgac tctaagatca ctagtatgcc gatgggacac
    32281 atggtagaag ctgccttaga cttaaatgat gaaggtcagt cccttgatga aatacttgca
    32341 catatcgatc gaatccgaga taatacctat gcttatctga ttgtagatga tttgaacaat
    32401 ttagtacgag gaggacgttt gacaaatggt gcagcgctga ttggcagctt attaaaaatc
    32461 aaaccgatcc tgacattttc tgaagggaaa attgttttat tcgaaaaaat ccgttcaagt
    32521 aaaaaagcat ttgctcgagc tgaagaaatt attggtagaa gaaataacga aatacaaccg
    32581 ccagtcaaac tttacgtgat acatgcaaat aatttagaag tagcgattgc ggagaaagag
    32641 aaattgcaga aaaaatatcc aaatgcagaa atcgaaattg gtcatttcgg accagtcatt
    32701 ggtacacatt taggtgaaaa agccatcggc attgccattt cagcgcaata attcctttta
    32761 ttcaaaaaac ataccaaaaa ta
//
LOCUS       DARBDV010000020        32656 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000020 DARBDV010000000
VERSION     DARBDV010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.020"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            28..1626
                     /locus_tag="SCM56_000926"
     CDS             28..1626
                     /locus_tag="SCM56_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109745.1"
                     /translation="MLFLSFAYVIPLFMSKGIYHSVNQDTYFHLSRIIGLDNVWSSPV
                     NFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFITFLTMVVSYFSMK
                     QIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQK
                     WYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTFFLS
                     AGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLFLFLGLIGAF
                     IFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNGFSSLFIAYT
                     MSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLTLIAPEDAVAIAEN
                     YNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIELDNSNNKNTVLTTP
                     IYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTKLAIASRYLSIVTL
                     ILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(1771..2511)
                     /gene="truA"
                     /locus_tag="SCM56_000927"
     CDS             complement(1771..2511)
                     /gene="truA"
                     /locus_tag="SCM56_000927"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEG1109746.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(2702..3337)
                     /locus_tag="SCM56_000928"
     CDS             complement(2702..3337)
                     /locus_tag="SCM56_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEG1109747.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            complement(3484..4725)
                     /gene="sstT"
                     /locus_tag="SCM56_000929"
     CDS             complement(3484..4725)
                     /gene="sstT"
                     /locus_tag="SCM56_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEG1109748.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            4923..5921
                     /locus_tag="SCM56_000930"
     CDS             4923..5921
                     /locus_tag="SCM56_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109749.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            5934..7952
                     /locus_tag="SCM56_000931"
     CDS             5934..7952
                     /locus_tag="SCM56_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEG1109750.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(8012..8728)
                     /locus_tag="SCM56_000932"
     CDS             complement(8012..8728)
                     /locus_tag="SCM56_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HEG1109751.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(8955..9497)
                     /locus_tag="SCM56_000933"
     CDS             complement(8955..9497)
                     /locus_tag="SCM56_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEG1109752.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(9502..9729)
                     /locus_tag="SCM56_000934"
     CDS             complement(9502..9729)
                     /locus_tag="SCM56_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEG1109753.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(9808..11688)
                     /locus_tag="SCM56_000935"
     CDS             complement(9808..11688)
                     /locus_tag="SCM56_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEG1109754.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(11877..12491)
                     /locus_tag="SCM56_000936"
     CDS             complement(11877..12491)
                     /locus_tag="SCM56_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1109755.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            12633..13193
                     /locus_tag="SCM56_000937"
     CDS             12633..13193
                     /locus_tag="SCM56_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1109756.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(13498..13812)
                     /locus_tag="SCM56_000938"
                     /pseudo
     CDS             complement(13498..13812)
                     /locus_tag="SCM56_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(14066..15701)
                     /locus_tag="SCM56_000939"
                     /pseudo
     CDS             complement(14066..15701)
                     /locus_tag="SCM56_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16256..16819)
                     /locus_tag="SCM56_000940"
     CDS             complement(16256..16819)
                     /locus_tag="SCM56_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEG1109757.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(16834..17877)
                     /locus_tag="SCM56_000941"
     CDS             complement(16834..17877)
                     /locus_tag="SCM56_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109758.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(18010..18414)
                     /locus_tag="SCM56_000942"
     CDS             complement(18010..18414)
                     /locus_tag="SCM56_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEG1109759.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(18647..19282)
                     /locus_tag="SCM56_000943"
     CDS             complement(18647..19282)
                     /locus_tag="SCM56_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEG1109760.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(19548..20600)
                     /locus_tag="SCM56_000944"
     CDS             complement(19548..20600)
                     /locus_tag="SCM56_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1109761.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(20613..21551)
                     /locus_tag="SCM56_000945"
     CDS             complement(20613..21551)
                     /locus_tag="SCM56_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEG1109762.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(22002..22652)
                     /locus_tag="SCM56_000946"
     CDS             complement(22002..22652)
                     /locus_tag="SCM56_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109763.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(22752..23261)
                     /locus_tag="SCM56_000947"
     CDS             complement(22752..23261)
                     /locus_tag="SCM56_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HEG1109764.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(23462..24331)
                     /locus_tag="SCM56_000948"
     CDS             complement(23462..24331)
                     /locus_tag="SCM56_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG1109765.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(24351..25472)
                     /locus_tag="SCM56_000949"
     CDS             complement(24351..25472)
                     /locus_tag="SCM56_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HEG1109766.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(25474..25869)
                     /locus_tag="SCM56_000950"
     CDS             complement(25474..25869)
                     /locus_tag="SCM56_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEG1109767.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(25888..26694)
                     /locus_tag="SCM56_000951"
     CDS             complement(25888..26694)
                     /locus_tag="SCM56_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG1109768.1"
                     /translation="MNKMSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALS
                     PILRKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKV
                     GTMGPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEF
                     GYKQGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQP
                     MLDKILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(26687..27475)
                     /locus_tag="SCM56_000952"
     CDS             complement(26687..27475)
                     /locus_tag="SCM56_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1109769.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(27489..27965)
                     /locus_tag="SCM56_000953"
     CDS             complement(27489..27965)
                     /locus_tag="SCM56_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1109770.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(28037..30694)
                     /locus_tag="SCM56_000954"
     CDS             complement(28037..30694)
                     /locus_tag="SCM56_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG1109771.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKK
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            complement(30713..31117)
                     /locus_tag="SCM56_000955"
     CDS             complement(30713..31117)
                     /locus_tag="SCM56_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HEG1109772.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            complement(31349..31606)
                     /locus_tag="SCM56_000956"
     CDS             complement(31349..31606)
                     /locus_tag="SCM56_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HEG1109773.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            complement(31731..32063)
                     /locus_tag="SCM56_000957"
     CDS             complement(31731..32063)
                     /locus_tag="SCM56_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109774.1"
                     /translation="MPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGLDLKNL
                     LCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLMELKKY
                     SNTQTKFS"
BASE COUNT        10265 a         6485 c         4952 g        10954 t
ORIGIN      
        1 aaaaaccaaa taatagatat catttttatg ttatttctaa gttttgctta cgtcatccct
       61 ctattcatgt ctaagggaat ctatcatagt gtcaatcagg atacttactt tcatttatct
      121 cgaatcatag gactagataa tgtatggagt tctccagtaa acttcaataa cttcgatcat
      181 catggaacaa tgatgaatat tttttatcca tggcttactc tttatccagc ttttttattt
      241 tataaaatga tgggaaatct tgttttaagt tataatatct actatttttt tatcactttt
      301 ttaacaatgg tcgtttctta tttttctatg aaacaaataa aaaacaatcg ttatatttcg
      361 ctgttatttt cgattattta tacattttcc gcctatcgtg ccattgacat attccatcgt
      421 gcttcattgg gagaagctgt cgctttaaca tttttgcctt tgatactaat ggggtgttac
      481 gaaatttata ttagagatta ccaaaaatgg tactggttgt ctattggcat gacgctagta
      541 gtgtatactc atttactttc tgtcgctatg gtttctgtat ttattggagg aacgttgttt
      601 ctttcttttt atttttggga ccaaaaaatc gctagactct taagtctttt aaaagctacc
      661 gtcctcacct tttttttaag tgcgggattc ttaattcctt ttattcaaca atctagagca
      721 caagaattga aagtcccact gggaaaagaa ttaagtggta tggctcctag tgatatgctg
      781 acacatattt taaataataa ctataacaac tatactattg gattattctt atttctcgga
      841 ttgattggtg cttttatttt tataaaaaaa ttaactgctg atgatttatt tattttcttt
      901 cttggtgtat ttgttctttt ttgttcaacc aatttatttc cttggcaact ttttaaccat
      961 acaccagtta agagtctaca atttgtctgg cggttaaatg gtttttcttc tttatttata
     1021 gcctatacga tgagtatagt gatctattat attttttcta ctaaaaataa ttcatggaaa
     1081 ataatggtct ttacgtttct tgcactaata ttgcatctct caggagtaag caattctatc
     1141 catgctaaca aagattcatt aacacttata gcacctgaag acgctgttgc tatagctgaa
     1201 aattacaatc atactgatta tgcaaacaaa gagtcaatct atcatcctga tatgattaat
     1261 aatgatcaat atttactagg taaccaggaa ataaatccaa caactcattt tatgagtaac
     1321 aaacttacaa ttgaattaga taattcgaat aataaaaata ctgtcttgac gacacctatt
     1381 tatcgttata aagggcaagt ggcttctatt aatggcaaac tagtacaaac aaaattatct
     1441 aagtttggca caactgaatt aactattcct ccaggtataa ataaagtcgt tattacttat
     1501 cagtacacaa aactagcaat tgcttcaaga tatttatcta tcgttacttt gattttattt
     1561 ttactttatc gatttacttt ttctaaacaa aaacaaccga gaagagaagt acaacattct
     1621 cactagattt ttatttaaga aagacttttt agctgtacac ataaacaggc taaaaagtct
     1681 ttttttatat ttctttaatt tgcttcatac attatttaac atcgatattt atactgtgaa
     1741 acatgcgttt catccattat caaagtattt ttatcgataa taaacttcat ccaaaaacag
     1801 accttgtgca gggacggttt ctcctgcatg ttgccgcact ttgctttcaa atatttcatc
     1861 gattgtttct atcggcatgg cacctgtgcc gatttctagt aatgtcccca tgataatccg
     1921 caccatttta tataagaatc cgtctccgac aaaagtaaaa tgaagcattt ctccctttcg
     1981 ttcgatagat aattcatcaa tcgtacgcac tgtggatttt tttgattttt tcaatgcaga
     2041 aaaaccaatg aaatcatgtg tacccactaa tttttcgcaa gctgcattca gcttgtccat
     2101 atccaactct tgagggaaat aaaagctgtg gtttcgctca aatgctgaag gaatcacgtt
     2161 gttccaaaca taatagctgt attttttgcc caccgcatta tatcttgaat ggaagcgctc
     2221 aggcatttct tcgattcttt tgatgacaat atcccgcgga aggtaacgat tcataaagtc
     2281 gatcatttct gccagtccaa tttctgtgtt tgttttaaaa tttgctactt gtcctcgcgc
     2341 atgggtgcca gcatctgttc ttcctgatcc aataatttct atctcttcat ttgtcatctg
     2401 atgcaatact ttttcgattt ttccttgaat ggttttatcg tcatctccaa gtctttgcca
     2461 tcctgaatat ctttttccgt catattcgat tgtcaacttg atgtttctca tattttagtg
     2521 gctcctctgt cttccatttg gtcttctctc taccttttta agattgtagc acaaaatata
     2581 gctccttgct tctctaagac agaagctata caagaaaact gtggaaatat atatttcaga
     2641 aaaacaaaaa acagccaccg gatgaagtat ccgctgactg ttcaatttga cggctcaagt
     2701 tttactgttt gagatgcaag atactcaacc tggcaatggg cacataatta aagaaaaaac
     2761 tgattctttg gcaaaaacct attttattca gtaatggcca agaatttata gaaggaattc
     2821 ttgccagtcc ataaatcgcc gcaaaaagca aactgatcac agacagtcca aggtataagt
     2881 ggcttacgtc catttgccca ttttttcgat agatcaagaa aagaagaact ggaaacaata
     2941 aaaaaccact gtaacctaaa cctgatccga tattatggac ccatgtcgag acattccacg
     3001 aagattcatg ggtattgata ctgaacaaac ctgtaaagat acaatcacct ataccataga
     3061 acgcgatggc tgctgtaact agggtagcta acacacgaga tgtactaaca aatgcagcat
     3121 acaatgcagg caatgacagg atgaaaaaga tccctgaaac cacagaccaa ataagaaacg
     3181 cttttcttac aggactggaa acctctccaa atacactgat cacgctcttc atttgatcta
     3241 attcaggata atacaatcct aatacgtacg gagtcaaaaa atcactcagc acgcctaata
     3301 ataaaaagaa aaaaccaaat ttacgtaaaa aatccatgat acgcctccaa taacaattac
     3361 ctatgctttt aattgttatt ataaacgact ttatcttgat ttaacagaaa cctctattta
     3421 cacgaacatc tatataactc attaaaaact cgaatcattt taaaaatact aaatatctaa
     3481 cgttcaacgt tcacttaatc catctaaaca atctttcaat ttgatttttt ccccacttct
     3541 tttacgcgcg cctagctctc ctattgcagt aaacaacaga tcacttgacg aatttaaggc
     3601 agtttccatt gaatcctgga taacaccgat aataaatcca ataccgacga tttgcatcgc
     3661 gacatcatta gagataccaa acaaactaca tgctaatgga atcaataaca gtgaaccgcc
     3721 cgcaacacct gaagctccac aagcagctaa agcagacaaa atacataaaa ggactttcaa
     3781 aacaaaaggt gcttcaatcc ctaaagaatg tacagcagtc aacgtcatca aggaaatggt
     3841 aatcgcggca ccgcccatat tgatcgtagc acctaacggg atcgatatgg aataagactc
     3901 ttttgtcaat ttcaatcttt cagccaacat catgtttatc gggatatttg cggcagaact
     3961 tcgggtaaag aaagcgggaa gtgcgctttc tttcaaacaa aagaaaatca atggataggg
     4021 attttcttta ataaacaaaa acgtgaggaa tgggtacaca actaatgcga caaacagcat
     4081 cgtaccgatc aaaacaagta aaagctgcag atactctgcc aatccagcga ttccagtcgt
     4141 agcaattgat tgataaacca atcctaaaat ccctatcgga gcaaatgcaa taatcacttg
     4201 tacgacttgt gacaacgcta tagatatctg atccaccaac tcttttgtcg attctgaacg
     4261 tgctcgaaaa gctagtccca acaatacagc ccagaataag acgccaaggt aattcgcatc
     4321 aataagagat tgaacaggat tttgtgtgac attcgtcaac atagtagata aaacactttc
     4381 caaactttgc ggtgcttttt cagatactgt ttcttgtaaa gtaataggaa cagtaaaaat
     4441 tgaacttaca ataactgcta caagtgcaga aagcaatgtt cccactaaat ataagatcaa
     4501 aataggcttc acgaatgtct tattcccaat tttatgcttg gcaattgacg ccatcgtcaa
     4561 gaaaaaaacc agaataggtg cgattgcctt caagcaactg acaaacaacg ttcccaatat
     4621 tgaaataaat gtccatgtgg ggaaaaagat accaaacaat attccgacta aaataccaac
     4681 aacaatacgt tgaactaacg acatatccaa aaacttctct aacaaatgac caccctttca
     4741 caattttaac aatcattgta tcattttatg caaagaaact aaaggtataa ttttctaaaa
     4801 attctaacaa tacaaacaac catttcttgc taattttgta ctttttctgt ctacacgttg
     4861 ttttttgaga tattctaagt taggatgtta ttaatgggca acttatcagg aaacgttttc
     4921 ttatgttttt gtccaatttt tttgtgaggt gtttattctt gaaaaaaata aagaaggaga
     4981 gttatggctt cctgacaatc tttgtcgctt gtataatgat ggttttacag cgtatattga
     5041 tttcagcagt cactattcct acatatgcaa caaaccctgg tcgattctac atctatacat
     5101 ttttacaaac ttccgttgtt attggtgcaa atttgatccc actttacatg ggttaccaat
     5161 atcaaaaaat cgcccgttta aaagttttac attatttaag tcgttttgcc tttatattcc
     5221 taatagccac actagtttgc aatatctttt tttacgtaca agctggcagc ttgaatattc
     5281 gtgattattg gcttatccta acacctatca gtcagaatta ctttacttat gctgtttctt
     5341 gtactttgct tttttgtagt attccttata cagttggatt tttaaataaa ttatcgaaaa
     5401 caacaattaa atatctacta ttatttttaa caattggatt agttggctgc tctactctat
     5461 ttaataaaga cccgtttgca ttacagaatg gacagagtat tttatggatt ttttatttat
     5521 ttttaatggg ttatggtttg aaagaatgga actggaaaga aaaggtccgc tataagcttc
     5581 ctcaagtatt tatcagcttg attgttttat tcggtttgat tattttgatg acacaaattt
     5641 cactcatcat cagaggaaat acagatacag cgctgaggtt ttctaaacca tattctctct
     5701 tcgcaatgta ttatactttc tcttcgtttt tatttttaga aatgctatct gataaattag
     5761 gtataaaagg aagaggaaat tttcttgctg tatttttgat tgtcactcaa gttatatgta
     5821 atttcccttt agtttcttat cgcgtatcta catttatgaa acgtgaattt ccaaactcag
     5881 ggaaggcatg gctgataaat atcgggcagt tttttatttg atatttatta gcagtgtccc
     5941 tcctagttat ctttttattt tatatacaaa aaatccgttt cattcatcgt ttcatcgaat
     6001 acttcgcttt tgattcgatt gaacaattaa ctcagcgttg gaaaaaaaga attcattggc
     6061 tttttgttca taaaagttat tttttagttg cagcattttt ttactgcttt accttcgtgc
     6121 agattttcgt tttagaacca aaagaaggtt ggaaagaaac tatccaggtt gccttaaaaa
     6181 tatttactca gcgacaagca cccatgatcc tgacaatcat cattattatg ggatttttct
     6241 tcttactatt gttgatttcc aatcgttttt ggtacgcctt gactgctacc ttgatcatta
     6301 acttactgct cactatttcg acagtaatca aaatggaaat gcgggaagag cctgtctttc
     6361 cttcagattt aaaaatgctg accggattgt cagaactatt atcgatggtt agcccagtct
     6421 tattaatcgt gggcggattg atccttctcc tattattgat tactagtatt attgtacaac
     6481 gtagactaca aaaacagtat tccttgaaga ttcattggaa acgtcgattt atcagtatcg
     6541 ctgtattatt aggatgtttt tcgggtgtct ttttcattaa tcacaaaaat tcgccttctt
     6601 tcttgctgtt taatttattt aaagtaaaca agactttctt taaccagcaa ggcgccgtaa
     6661 aagataacgg accagtcatt caattcctaa acaatatcga cattaaaatc atggaagaac
     6721 ctgctggcta ttcgaaagaa aaaattaatg gaataatgaa aaagtacaac acaaaggcag
     6781 atgaaatcaa ccaaacaaga agtgactggt taaacaatca gacgatcgtt ctcaatttaa
     6841 gtgaaagctt cagtgatcct gcacgtgtcc ctaatctgac agtacctaca gaccctattc
     6901 cttacatcaa aagcattata acagaaggaa caggaggatt gatgttatcc gtcggttatg
     6961 gtggcggaac agctaacatg gaatgggaag gactgacagg cttgagtatc agtaacctgt
     7021 ctgcttcgtt agttacacct tatacccagc tagtggagcg tcagaaaata agtccaaata
     7081 ttaccgatct attcgatgaa aaaatagcca tccatccttt tacagctgct ctttatcgca
     7141 gaaaacaagt gtttgataaa tttggttttg acaggttcta ttatttagac agtccggata
     7201 aattaactta tacggataaa atagaacaaa gcccacgcat atcggatgat tcagcataca
     7261 aagaaacctt gaaagcttta caaagcaata cggaaacgtc acaattcatc caattatcca
     7321 ctatgcaaaa ccatatgcct tatgatgatt actatagtga attgaactac actgctgaag
     7381 gaactgctgt cattcctaat agaagaaaag aattacaaac ttatatgcaa ggccttcact
     7441 ataccgatac agcagtaaaa aaattcatcc aagaaatcga caaaatcgat aaaccgatta
     7501 cctttgtttt ttatggtgac catttacccg caatctatag tgggaatgat tcgaaaaaat
     7561 atggattaga acagcaccag acagattatt tcatttataa caacaagtat agtcgtgaac
     7621 aagcagctac gttaaataaa gaagttgttg ctcctaataa ctttggtgct atggctcttg
     7681 agcatgcgaa tatcaaagta actccttact atgcgttgtt gacagaagta tctaatcatc
     7741 tacctgcatt aacaattgat cctacagaaa gcttgtctaa tcgttttaat ggaaaacagg
     7801 tctttgttac agaggatagt caaattatcc aagaaaaaaa tttaactgag aaacaaaaag
     7861 aaattctaga agattatcgt ctgatacaat atgatcttgt tgcaggtaac cagtacagtg
     7921 caacatgggc gacacaaaaa ataaaagact aacaaataaa ctgagcaagg ggttgtgata
     7981 aaagacatgt cacaacccct tgtttatctc attaatttgc ttgtccataa acgatttgat
     8041 taaagccatt tttcaattca ttattcgctg tctcgttgaa gtaaagataa ttatcgggat
     8101 tcccctgatt ttgttgtgtc accaatactt ttggctgatt ttgctgaaac ccgaatagat
     8161 agacgtgctt tttcagatat ggttctgtct ctgcatcgga atagacggct gtgatttgat
     8221 agtctgcttg acctgtccca ctttctgacc attctgcaga aacaggcact ccaccaatag
     8281 ccatcttcca ctccccgttc aaaatagctt gagggacttt aagaccatat aagtccacac
     8341 tcatttgatt cgtataactt ttgtattgtt gtcccattgt ttggccccat ttggtcataa
     8401 atgcctctaa ttgtgcggat ttgtctgcat tccacagtgt ttgttcaact tgttcgctgc
     8461 tttctgaaga ggaagatgaa gcctctacag aagaacttga agtagatgtt tgagttgttt
     8521 cagtcgtttt ggaagactct gacgaagtag aggtatctgc tgtttctgta cttgctgcag
     8581 ttgacttgct ctctgtgcta gttgttgtag tagttgcaac tgttgctttc gctgtttctt
     8641 ttgttgattc ttttttcgat tcattcccgt tggtacaacc agctaacaaa aaagctagac
     8701 caattgttac taaaatcctt tttttcaatt aaaaaactcc tttcgattac tcccacttct
     8761 ttattctatc aaataagcaa tgtgatttta ttgatttttc gtattttctc atcaatcttt
     8821 caaatcttct aaaaactgag aagcacccgc tgatgttgtc tgtaatacat cagcgggtgc
     8881 ttcttttatt ttaatcttcg tgattttcca atttacctca cgctttatat ttgtatttga
     8941 agaaacatag acttttagtc atcatcgtct tcctcatcca aatcttgtcg gacagttttt
     9001 ccttggagtc tttgtacttg gtagatgatt tctttattga aacttaaaag ttcaacaagc
     9061 aattgttgca agccatattt ttcttctttt tcagctaatt tgattgcccg tgtgataaag
     9121 acattttgga aatcaaaatc ttttgcaaag ttttccaaca ttgttgaggc tggtaaatat
     9181 ttgttttgac catgttcgta aatttttcca taacgcatga cttcttctaa agtagacgga
     9241 acgatttcat tttcaatcac taactcatga gagattttat tgaaccatct cgcttcttga
     9301 tcagcatatt ctgaaaaaag ggtacttaat tcttgcgaag atgtacccaa aacatacaat
     9361 tgtgcttgga tcaatttcat ttcttctaat ttaaaatttg ctaatacatg ccccaccatg
     9421 gcgccagcag ttggtttatg atggtcgata tctgcttgtt ttgtttcttg aacaaatttt
     9481 tcttccacta tattcattga tttacgcctc ttttactttt gctgattgga cttcataacc
     9541 taatttttcc acaactgcag ctaattgttc agggtggact tcatctggat caaagtttgc
     9601 tttgattttg ctcgcattaa acaaaacttt cgtattgctt accccaggct gttgagaaac
     9661 cgcatgttcg attttttgca tacagcttgg acaagctagt gtatctaatt gcataataat
     9721 ttttttcatc gtagttcttc cttccttttt cttgatagtc ttattctaga ttacagcttc
     9781 cataaaaaaa ttgattctta tcaagtttta cgatttttct tctttgcttt cgtgtccaaa
     9841 atacaataaa cgcatcgcgt taaagataac gatcaagata ctagcttcgt gaacgaacat
     9901 cccactagcc atgtaaataa acccggcaaa taagccgttg agtaaaaata agacgacacc
     9961 aatggcaatc actacatttt cacgggtatt taaaacagtt ttcttagcca agcggtaggc
    10021 atgaaccaac gattcaaaat tagattgcat caagacaacg tcactcgttt cgatagcaac
    10081 atccgttcct gatcccatag caatcccgat atcagccgca gccaaagagg gagaatcatt
    10141 gattccatct ccaataaaag caacgcgcaa tccttgttct tggtattttt tcacaaattg
    10201 aaccttttcg tctggtaata attcagcatg gacttcatca atgcctaaca tatcagctac
    10261 ataatcagca gtcatctgat tatcacctgt cagcatcact aactgttttg ctcctgcttg
    10321 tttcaactta gctaattgtt cagcgacttc tggtcgaatc acatctgaca cacctatgat
    10381 ttgagtgatt tttttgtcga ttgctacaat aatgaccgag ctgcctgttt gttgtaattg
    10441 atgtaaatct tgtacttgct ttggtgtgag agaaagtttg tgagcggcaa tcaatttggt
    10501 gttgcccgca caaactgtat gaccatcaat ctcagctgtg attccttgtc ccttgattgt
    10561 ctgattatct ttgactgcta attgttgaaa atcaatgtct ttctcctgag cataatggat
    10621 cacggcgcgc cccagaggat gatcggataa cgtttctaat cgagccacta aagctaagct
    10681 gtccccttca tcctctgtat agttcttgat agtggataca gcagtagttc ctttggtcaa
    10741 tgtaccagtt ttatcaaaga caaaagtatc gattttggaa aaagtatcca ttgcttcacc
    10801 gcctttgact aagatgcccc gtttcgctcc attaccgatt cctgctacat tcgaaacagg
    10861 cgcaccaatg actaaagcgc caggacaacc aagaaccaaa acagtgattg ctaaacgaaa
    10921 atcacgacta atcagaccaa caaccaacgc aataagtaaa acggttggag tataatactg
    10981 agaaaatcga tcaatgaatt tttccgcatg ggatttggta tcttgtgctt cttctactaa
    11041 ttcgataatt ttggcaaagg tcgtatcatc tccgacctta gttgcttcaa tcgtcacgta
    11101 gccatctgct aaaatcgttc cagagaagac ttcatctcca ctgattttga cgacttgtcg
    11161 tgattcacca gtcactgcgg cttcatccgc ataccctttc ccgtcaatga ctcgtccgtc
    11221 cacaggaata gctccaccag tttttactaa gacgatatcg ccttcttcca cgtcatcgat
    11281 ttccacttct tcagtcgttc catcttcttg gacaacacta gcagtagatg gggccatctc
    11341 ggtcaaagcc ttgattgact cccgcgtttt ggtcaatgtt ttttgttcca gatagctacc
    11401 aaacaaaaac aagaatgtaa cgatagctga ctccttgtat tctccgatca taaaggcacc
    11461 aagcacagca atagtaacga gtaaatcaat tgagataact ttgactttca ctgcctgata
    11521 cgcatggatc ataataggta tgaccgcaat aatagaagct atggctagag caatgttata
    11581 gaacaagaca aaaccagtaa attttccgat aaaaccaaaa ataatcaaca cagctgataa
    11641 ggcggtgata tgatttcgat gctcttggat ccacttttgt aatttcatct ttctccctct
    11701 tttcctttca ttcttacgag actaagtata gatacttatc catcataaaa aattgaccac
    11761 tatcaagttt agtcaaaaag gcggttttct tttggtagaa tacactacaa aaaatagtta
    11821 gcaagaagac tcactaaaag tgatcttgcc aactattgtc gtttctatcg aaattttcat
    11881 tgaatgattt tcctgtacag catacatcgg agatctattt ctttatctaa gatttttatc
    11941 ttgctaattt tttcctttag tctttcaaat ccacgtcgtt catagaattg atacgtacag
    12001 ccactatctg taaaaaggaa tatttctttt cctttctctc ttttttcaaa ttctgttagc
    12061 aacttggtac cgatgccttt tgttttcaaa tcaggatttg cagctaaaaa aatgatctct
    12121 ccatctggcg aatgcttctt taaatatgct gcaagcattt gttcatttgc tgtctcgtaa
    12181 gctcctttac tttctttagc aaatatattt tgtaaaaaat cgaatatctt aacatatatt
    12241 tgctgtccaa aagaacgatg tttctttttt tctcctttta tctccgccag caaaacacct
    12301 gccaactcgt cctctgcata caaagcaaga atttgagttg ctcgattcaa ctccatatac
    12361 aaaaaatatc ttccgtaaag attcagcaga agttcatttt ccatatatct ctcaaagttc
    12421 attccttcaa cagcaaaata gatagctttt ttgtaatctt tcttttctaa gggtttaaaa
    12481 gttagtttca tcatttcgcc tcctcaactt caatgattgt gttatactta cattataata
    12541 gacacagtgt ctactatcta taatcaatca tctataagtt tgtcgattaa gcgacagctt
    12601 tgctttgata tgtcggataa ggagataggt aaatgaaaaa gacagataga cgcgtcaaaa
    12661 aaacagaaaa agctttggct gaaacactat ccacattatt agtaaataaa aagattcaag
    12721 caatcactat acgcgaacta acagaaacgg cagatgtaca tcgttcgact ttttatacac
    12781 attataagga tatctatgat ctatatgatc aattagaaag caactttttt cgggatctaa
    12841 atgaaattct ctcttatgat ccagcccata gctatgagga actatacact cgcttgattg
    12901 actatgttta cgcaaatact tttttatatc gcttatttat cctcaaccat ggtgatattt
    12961 cgtttcagaa gaaagttaaa caagttattg aagaaaacta tctgaacata tggctatatg
    13021 aagacaagaa aacgattatc acagaagaaa tgagattctt tactacgtac cacgtccaag
    13081 gttgtctatc cgttctcaat cggtgggtag aaagcaatta caagtactcc aaaaaagaat
    13141 tattagaact cttacagaaa ctgaatgatc acatagagaa aatcatgcct tgaataaaaa
    13201 catactaagc ttagttaaga agaaccgtaa ccttctcgta taacaactga atcttattta
    13261 gccaaattgt ttactgaaga ttttttgagt aaaatttcta taattcctac ttattgaaaa
    13321 gtagtttgta gtgctgcttc tccctctcta agtgatatat cttgttgtac caatcaaatt
    13381 cacctcatca ttaataaatt acttttaggc taatttatta caacttatag gttgaagctg
    13441 taaccaattt atagtcatat tatattttta tcatccagtt tattgaattc ttctcgttta
    13501 cctcaattca ttttcaaggg gaatcagctc atcatctaac attttacgcc atgctcgctt
    13561 catttcttgt gggtatttaa atggtttcgc ttctggatgt ttggctttat atccttcgta
    13621 atccttactg ttgataattg ctttcatatt aggaattgga tcttcacctg gcatatattg
    13681 cagattagaa gcatgtaatt ttttctcaga aacaaaccac tcattggaaa agacatgaat
    13741 caaatcatca cttacttgtg taaaatagcg acgtttaatg acaaagatat cttcattgct
    13801 atctagtgta ccttcatttc tttgtgctta aattcaaaga taattttctc aataaagatt
    13861 tttgattcca gtaagattcc tttactcctt ttacaacata gcgttgtcct ttgttttgaa
    13921 acatcttctt cccctctttg ccattaaaaa tagcccaatt ctctataaag aagattaagc
    13981 ttcattatta taatataaag tttaaatttg gtttattact tactttttct tattctctag
    14041 taagctaata ttgaaaaaaa tgtaatcaga tcattatttt tatatgttgt aacttcctga
    14101 taagaaaact aattaaaaga aagaggtaac tacataaact aattaaaaaa gctaacttca
    14161 tagttggagg ataaatggaa gaaacttcaa tgattgtttc tccttgtgtt ccttcaatcg
    14221 acactagtcc tattatcaat ctctcttttt tgtccattta cttttgcgac tgttccccaa
    14281 taatcaaata caggaagcgt tattttttgt cctttagatt catgtactgt atagactatt
    14341 ttcgaatcca tcacttgata aggcatagta tgccattcct tgccaattct tacttcatga
    14401 ttctgcaaat gttctttgta ttgtaatgct tccgtgggtg catagtcata aagtcctccg
    14461 ggaaggagtg tcgtgtaacg aataagattt tcttgattca ttaccaagtg atctctctga
    14521 gtgacaactt tatgttcata gtttgctttt gttgcagcag atacagccaa cagtaataaa
    14581 acaattgccg ttatattaac tacttttctt tgcttagctg atgtgctctt catgaaaacg
    14641 aacgataaaa tcaacgagcc aaacagtact tgaaagccta agattcgcca aggaaattga
    14701 atcatttgaa tgggtgtatt ttgtaataaa gaccatggaa aaaatttggt ggataagagc
    14761 caagagacta ccgcactaag gtaaatcact ttttctttgc gattaaatcc tttaaaaaag
    14821 aagagtggac agatcaaaga aataatacca atgattccta ccgtaaaggt tgtcaggtca
    14881 ttattcatcg caccaatcaa actatccgag acatttaatg cttggtgttg taaggtcgtt
    14941 ttatctggct cattaatcga ttgaaccatg atttgtcgga acatcggata ccaaaagtat
    15001 gctgttaaaa gaatcgtcat tcctactgcc tttgacaact ccacaatcaa ccggatgctt
    15061 tctttttttc ttgtttcact tactacaaag ccgcctaaga ctagtaagcc aacggctatt
    15121 acactcataa aagtggaaag aacatgtgta taaatgagta agctcatact gactgctaat
    15181 ggaattgcac atggcttttt ctcataaatc agtgcgtaag cataatacat aactatcggt
    15241 ataaccgtaa gtgcaatcgc ttcagcaata gcagaacgat gataaatatc aatcgtacgg
    15301 tacccataaa atgtatataa acagctaaat ataaaagccg catcgattct ttttaaaaat
    15361 tttagtccat aatgaaacat cagcatgata gtacaaacat tcaatagcca aacatataag
    15421 atataactaa cgattaaatt attacttatc caataaaaaa tgactgccgg aaaaaaggtc
    15481 aagtagggat aaaaataatt cactccgctc ccataatgat taaacgtcgt aaaattgatt
    15541 ggaccagaaa aaattgaaga taaccctttg actctagcaa aatggaaggc aatatccccc
    15601 gatgtagaaa tagtatgaac atttctaaaa taattgattg aataaagtcc agcaataatt
    15661 gctaggagaa tggacaatat tatttttttc tttacttgca ttacttaatt atatactcct
    15721 ttcatggatt gctttatcta gtataactta aattttaaat aaatcgttat ttatttttac
    15781 tctcacttaa aaaagcacta aaattataag agagggaggt aatggtctga acaccaagaa
    15841 ttgttccaac atcgaattgc ttaactaatg agttcaaagc ttaattatgt ggctcttata
    15901 tttaccgagc gtggattgcg atctctttag cgaaacaaac atctttcata aaaaaaacag
    15961 ccatatccgc tttcttggat atgactattt actactactt tactatcttt cattcttctt
    16021 gtctcactta tgataaggcg ctgcgtatca tgagtgaatg atattaggaa aacttcataa
    16081 aataaatctt ttatacattt ccacttctag atggtcattc atgttagaag aatcaaacta
    16141 tttttttact gtcgtaataa tattaaaaca ctagaacaaa tcaattaaat tccataaaat
    16201 ataaataata gacctagcat catctggtat aaaatggatg ctaggtccgt tatatttact
    16261 caaaattgat tctacgaata gatcttctta tcatttctat tttagtagtc atgcgttgtt
    16321 gttcttctaa aggtggtaat ggtataagta gtttagataa atttgtacca tttacatttg
    16381 gttgcccagt tccgtaagaa attttttcta ataacttcca atataatggc gagtctaaga
    16441 agcagtctac gtattctgat aataatttct caactaacct aattcgaatc aagtaagaag
    16501 caaaaacaga ttcctcactt atttccttaa ccaagaaact ttttcccata gttcctcctg
    16561 tccttgctat caaaatgtca ttttcttcca atcttaaatc aactagttta gaattactaa
    16621 tatcacagta aggcacactg gaccaattta cacgtccctc ttgtatatct gtgattctca
    16681 aaaacttaac attgccttgt tttttcgccg aatctgtata gccatattga atcttagtag
    16741 aaatacttcc taaataaacc catagtcgaa catcgctaga tgatttttct tgtcgttctt
    16801 tatgaacaac agtgatcatc ttgagttgtc cgactatttt ttagttgcag caatcttttt
    16861 gggacgctta tttgcatgaa gcaacgttgc taagtctagt aataaaacat tggctaacac
    16921 aggattggct ttctcgcgat acgctgcggc gccactttca aacaatactg ctagacgatc
    16981 ttctacaaca acgatttgtt ccaaatacgg tggaaatttg attcgtttta ggatccggct
    17041 aggagagtcc cgctcggaca ctaagagttc cgaggtataa ggaccataag aacgactggc
    17101 taagagataa tggccataaa aagccacacc ttgaatctta tcagatgttt ttctgctcat
    17161 tgtcacggtt ggttggattt tgtctttgcg gcgaattgct gttttcattt cagctatcgt
    17221 cggcaataga tagaaagtca actgtccttc accttttagt gtgaagttgc cagataccag
    17281 ttgatcattg ttttgagtca aatacgatgc ttgtggaatc tctgagagat cagttgtcag
    17341 ccaataagcg attggctgtc ccgtctcttc agatgtatct gctaagatat cgatcaaacg
    17401 taaagcggct actcgtccag tagcagatcc tgtgattgtc aaccataaaa gttcccgttt
    17461 tgtatcatat actaagcccc ctacatgtgg ccgtttaggt aaaatcagca gtttgatcgg
    17521 ctgtcctgtt tctctatcca gtacatgaat aatagaatga tgctgatggt catagcagta
    17581 ggcagaaata aacacaaaat cttgagaaaa agccaaccct tgcggcgtca tccattgaca
    17641 aacctcattt tcgccggttt ccggcaccac cattcttgtt tctgttaatc caggaatgtt
    17701 atattgtttt gcttcagcct tttcccaagc tgcttgaaca ataggatacc gttttaactt
    17761 tttttcaata tcctgatgta tccgttgctg cttccaatgc ttaaacttta ggacatagta
    17821 aacaagaagg ccaatgacca gcaacactaa aatcattatt gctacccgct gtgtcatttt
    17881 tttgcctcct tgttctattc gatcaatcgt ccatataccg ctattaatta ccgtaactac
    17941 atgtttcttt tactagcgtt tctttatagg atctttcata atctcattta tagtaacatt
    18001 aagaatccct tattctacta tagtcacttc cacatcaata ttgcctcttg ttgctttaga
    18061 gtatgggcaa aatgcgtggg cttcttctgc gattttctct gcttggtcct tctcaagtcc
    18121 tggcaaagaa acttctaaaa cgaccccgat ttggtagccc ccatcttcta attgaaataa
    18181 agatacccgt gctttgactt cagaatcaga ctcaagatta ttttcttcca gcatgtgttg
    18241 aaccgcgccg ttgaagcaag atgcgtatcc agccgcaaaa agttgttccg gatttgtccc
    18301 ttcactatgg attcctgggc ggtcgatctt catgtgcata cttccgtttg gtgcctcaac
    18361 ttcgccatca cggcctccac gattgatcat tgctgtttcg tacatttttt tcattgagat
    18421 catcctttct ttcgtcgttc tatattaagt gtaacgagta ttttttaatc tgacaaaggt
    18481 aatgcttgca ttcggcgaca tcagttagac aacatcgatg atcagtgata gaagcagttt
    18541 atctatacaa aaaaagaagg gttctatgat atttggtgtc cttactcgcc ttgagtaaca
    18601 acaccaaata tcatagaacc acttaatcag cttcacactg ctttttctac tttcgtagtt
    18661 gaaaaaaact taaacaaatc atcggcaaat agttaaagaa aaaacttaat cgttgaaaga
    18721 agcccaattg cttaaagaag gcaaactgac taatcgacgg aatccgtgct aatccgtaaa
    18781 gacctgcaat tagtaaacta ataactgtta aaacaagata aaaatggcta agcgtacctt
    18841 gaccactttg acgataaagc agaacaagta acaatgggaa aagtagaaag cctgcatagc
    18901 ctagcccaga acctatatta tggatccaag tcgatagatt acaagagctt tcattcgtat
    18961 tgatactgaa taagccagta aaaatacaat cacctatccc atataaacca actgttgcaa
    19021 caacaagtat tgcaagtgtt tttgatgttc caacaaatag gtgatacaac gcaggcaacg
    19081 aaagaacaaa aaaaagtcca gatacaactg accagaccaa gaaagcacgt ctgactggac
    19141 tatctacgtc accaaacaca ctaattacgg tagtcatttg attcagtttg ggataaaaaa
    19201 ggccaaggat atacggtgtc gataaatcac tcatcacacc taataataaa aaataaaaac
    19261 catatttttg caaaaaaatc attttcttcc cctacttttt agtgattatt cgatacatcc
    19321 aacaatcaaa ttttttctca ttttaacaca tatttttatt ctagttgtct tttgaacaat
    19381 caataacata aaggtgagtt tcttctacat tttaaaaaat cgaatggcac tgaagagttc
    19441 agttgtcagc atcaatctcc tactcaaaaa ggcaaaaaaa cgtaagtcag atgttgttga
    19501 catctcactt acgcatccat tgattgaaaa tttcttagta aaatctacta gcctaaaaat
    19561 ttgaagactt cttctcgatc atacagttta aatactttct cgcataaaaa ttgagtaatt
    19621 agtgccgcaa aaacagggat gactaaccaa caaagaacaa caagaaagcc atttagccct
    19681 gcatctaaag aagccaacgg accaacaagt ccgaccacgc cgaaaccagc agaagcaggc
    19741 gtacctgaaa tagaaaatag agcgacagga atagctgtaa tcactgcact aactaaacaa
    19801 ggaataagaa tcacaggttt tctaaataga ttaggcatca tcatcttcat agcgcctaaa
    19861 gcaatcgcaa ttgtcacacc tgacttatta accttccaag agttcactac taaaacaaaa
    19921 gtggttgcag ctacgcccat cgcagccgca ccagcagaaa caccatctag ttggattgcc
    19981 attccgattg ctactgttga aataggagag ataatcaaaa aggcaaagga acaaccaatc
    20041 aagatagaca ttaacagcgg ttgtaaggta gtgaagttat taatcaatga tccaattgca
    20101 gaagtaattg ctgttacaaa agggaataaa taaaaaccaa gtaaacctgc acctacacct
    20161 acaacaatcg gtgttaacac aatcgacact gacccaaaac tatttccaat cagtaaaatc
    20221 agcccgacag caatcgatgc tgtcagcatc gtattaataa tatcgcctgt accagcacca
    20281 acgtaaacac ccgtttcacc caaatcaggt gtgaatttga ctacaccaga accaacaaaa
    20341 gaagctccgg ctacaaccat cattctcatc ggatcaaatc caaattgtaa ggcaatcaaa
    20401 ccaccaatga tcaaaggtgt tgctagttga aaaataactg ctaactgtgc gatcgttacc
    20461 gcaaagggat acgcactaaa atattttaaa atggcaccta atactgcatt aggaatcaat
    20521 ccaataatga ttcctgttgc tgtaccagcg aggactttat tcagaaaaat tttcggtgat
    20581 attttttgtg aagtttccat tcaatctctc ctttatttta cgttcaaact atcttctttt
    20641 gcatggacaa gctcagtcaa gaaatcacaa taaggcgtcg caacaccata tttcttccct
    20701 tttctagaaa ttgcgccatt gatataatcg atttctgtta atcgatggtt cttaatcaag
    20761 tcttgataca ttgaaggata atgtaaccca attgtttccg ggtcaaaaca agattcacaa
    20821 tgtgcaatga cttctgggac atctagttca atcttctcta ctgctgctac tttggcaaac
    20881 tcattgacaa tcgtcgccac cattttatga gcagtcgatg tttttcctag ctcagccata
    20941 ttaacgtcta agattgtgca tagtccgttc attgttccgt tcacacatgc tttgcgataa
    21001 atagaatagt gaatattgtc agaaaaatga gcatttaatc ctgattcaga caatttgtct
    21061 gctaattttt ttgcagctgc ttcttttcca tcacctaaat tttgtaattc tactgaacca
    21121 cttccaaata atttgacctg accaggacct tcaagacctg ccgtccacat ggtatttcca
    21181 atatagatat tttccattgg tacaaatttc tcaataatat cttcatgccc gataccattt
    21241 aatagacata agacctctgt atcttttttg attaatgatt ggatatcctg cagcattttt
    21301 tccagctgca tcgctttggt aaatagaata atcagatcaa cttgatcttc tttttctact
    21361 tcggattgaa gaacgattgg tagttttgct tctacctctt taccattaaa atttgcttgt
    21421 aatccatgtt ctttgatttg ttggacatgt tctgcccagc catcgattaa taatacttcg
    21481 ttcccacttt ggtgaagcat caacccaaag cgacttccca ttgcgccagc gcctgcaatt
    21541 gctattttca ttgattcttc ctcctttttc tttctattat actcatttga gaatcttttc
    21601 acaacctcgt tttgttattc ttctcttatt tatcatgttt atatccacca ttcaagttat
    21661 tttatcagca aatcaataaa aactggaata gaatcccaat ttcatcgtaa gagaaactag
    21721 actagcaata agaaaataga aacattcata gaaaaaatct cactcctctg attataaaag
    21781 agggggcatt aacctcgtgt atactaaaac cttccattca ttcgatggat gattttcttt
    21841 ttgtgaaaaa atattcattg tagtacgatg ttgcgcaaca cagacggatg gtattgctta
    21901 ttatttatta aaatcgaaca atacacttat ttaaaaactt ctttcccaga ttttcttcca
    21961 tttttacagt tataactagt attatcatga aaaatagcct tttattgtct atgggaataa
    22021 taatttaaaa ttggtacttt tttattaatt agttcatcgc ccaataatct ctccagtttc
    22081 ttagcatctc ctgactcaac cggaattatc caagcccagt ctaaataatt ttttaaaatc
    22141 tcatcatatt ttgcaaatat ttcattgcca tattccgtcc ttaaaattat attttcagtg
    22201 ccagacaagt gatttatatt gagttgataa aaaagatctc catctaaaaa aatatcatta
    22261 gaagaagatt tccccacagt aatgaccatt ccatcagctc tatgtgttac atacaaataa
    22321 ttgtatgacc tcatttcacc taatgaatca attatttctt gccctttagc tctaatatat
    22381 tgatctctat tacctatctc actcttattt aacaaaagtt ttttcatgtc aaatttaatc
    22441 ggctgtttta tttttgtaaa ttcgcttcta ctactaatcc aatccgagat taatttttca
    22501 acatgtccca gagtaaactc tgatttattc gctaaatact ttttttgttg tattaaagac
    22561 atctttcccc gaagctcaaa attgagccta catcgattct ctatctcttc aaaaagcatc
    22621 ttatcttcat cattcttata ttcatcattc ataaaaccaa tccagctcct cgattaagat
    22681 aatattttaa tctcatacaa aaactagctc tcatcaaaac aaccattgtt ttgatttctc
    22741 atgaaatttc tttatacttt tctccgatta taaattccta accccttgag aaaattacgc
    22801 atatatttat ctccgcaagg cttaaaattt cgttggcctt cttttctcaa aaatgccccg
    22861 atttcgcctt ttgatggata gatttctgcc tcagcaaaaa cgtctaaaat atcatcagtg
    22921 gtcatagaca aggcgatttt taattttttt agcaacacat tgttgatata tttgatttcc
    22981 ttgctttttt gcggcgtgtc tactatcggc ttatcttttt gcggaccacg cgccaagaga
    23041 atcaggccat ttaggaatgc gtcaaaaact tggttgttga tggttttttc atacacatta
    23101 ttttctgcat tatctttgtt cttatcctgt actttggtca gtaccgcctg agcttcttct
    23161 ttagtcagtg gtacaccgcc tagctcaaaa gctttaatca aatccgtatc tttcaaatca
    23221 atcgcataac gcaatcgcaa taacctatca ttgttattca tttgttcctc cgaagtattt
    23281 agtctttact ttcttacgta ctttttgtct tgctaagatt tttaggatca atgataaaaa
    23341 ccgcatcact tatgataagg cgctgggtat catgagtgaa tgatattgct gaatacaaat
    23401 agtccgacca cattaagttg atcggacttc ataagcattt tattgactaa tacttttttg
    23461 cttaagaatt gattctaaca tcttttgcta aagttaagca tcctatagtt cctgcattat
    23521 ctgctaactt aggtgtgaca atgtattctt cgatatttgg cgtatcgaca tagccgttaa
    23581 ttagttctaa aaaacgtctt tgtactttct ttttcagatg ttcttgttgc atgacaccac
    23641 caccaaaaat tataatatct ggtgaaagca tcagggtcgt attacaggca cattgtgcaa
    23701 tataatccgc ctcgatttcc cagaacggat cttctataga aagatcttgt cccttggttc
    23761 ccgtacgcat ctcgatagcg ggtccagatg ccaacccttc gagacaatca tgatgaaaag
    23821 gacaagtacc agagaaagta tctcctgaat gtctcttaac taacgtatgc cccatttctg
    23881 gatggctaaa cccttcaaca aaaatcccat cttgtatagc tcctccacca attccagttc
    23941 caacagtgta ataaacaaca ctggataaac cttttccatg acctgaaaca tactccccgt
    24001 aacaagcagc attcacatcg gttgtccagg caattggaat ttcgaaatgc ttattcaacg
    24061 ttccaacaaa atcaaaattt ttccaggcag tttttggagt agaggtgata tgtccatagg
    24121 tctttgattc acgatggata tcaatcggtc caaatgaccc tactccaatg ctttctaatt
    24181 gttctttata gtgattaaag aaatcaatta ccaaactcat tgtttcattc ggtgtagttg
    24241 taggaaaact cactctttct aaaaccttta aatcatcatt gccaatggca caaacaaatt
    24301 tagtcccacc tgcttctatt gagccataca tttcttactc caccgtcctt ttatctagga
    24361 attcgttctt ccctgtctaa tagtttttcg aaaaactcat gtggttcctt ttggtaccaa
    24421 taggcaacag aagaaatatc gtcttgacgc tcataaagct tgatgtcatc atttcctagg
    24481 gtttggaaag taacacgtaa attttctttg aaactaatgg ggtcgggtaa atgccagcgg
    24541 tacaacccat gcatcggtaa agcatcatcg ccaaaaccat gaactggatt tggattaggt
    24601 tttccagcac taaaataatc ccgggtgtga tctcttttag attgaaaagg atagcccata
    24661 aatagcgtat taaaattttg agcatatgcc ctttctccaa agttcttttt atggaaagcc
    24721 caagcgccgc caaaataatc ttctgctcct gtagatgtta ttgtagggaa atcttcatct
    24781 ccatctatat aaaacttaaa ttctccttcc ccccaccagt atctttccaa tgcgcacaaa
    24841 gcaaagtagg ttcctacata atacccagtg cctttgattc catcaataag ggtgtaatct
    24901 tttccttttg tcgtgatggg ttcacggttc catttagcat gaaaatacat cgcatcatcc
    24961 ggtacttcag tcaatgcata attaaatgta taaaaaatgt gcttaataaa ctcaggatgt
    25021 tcattcgtta aagtaatctt agcttttttt cgaaatggca tctcaaaata gcaattcatc
    25081 cctgatgttg gattcactac gattggtaag gaattcactt cgcatcttgt cccaaaccca
    25141 ttacaaaaaa aatctcctaa tggactttct acagaaggag tggattcatc atcccaataa
    25201 atacgcaaaa ttaaatcacg taagacgaaa ctcccagcga ttgttttgtc tgtcactgtc
    25261 atccaaaaat gacgtaaaat tccagggcct tccatttctg ctaaagtaat tgtctctcca
    25321 gcatttatcg gaacagaagg tgaccctttt ctcccaattc ctagatgact cgctgtcatt
    25381 gccgctttcc ctttttcacc atttttattt tctggtgaaa tagttctact ttcctctccc
    25441 ttgtggagcc atatattatt taaacaattc attttattcg tcctctatcc tttcttcact
    25501 tttttgatta ttcatataga tgatttgttg ttgtgcctcc gcaataattg ctccaatatc
    25561 taaattatca tttcctaact gcaaagcgat tgacattgcc aagggaagat tcattcctgc
    25621 aattacgtgc actctgtcag aaatataggt aaaaaacttt tgatttacac ttcctccagc
    25681 catgtcagtt aaaacaatta tttcgtctgt agtatccact aactctttaa tttgttcatc
    25741 tatatcatca atttcttcga catatgcaca taagctacta atttgattat gcatacctgt
    25801 taaaaactct agtgtctctt taaaaccctc agctagtatt gaatggctag caatgatcaa
    25861 tcttctcata attcaaatcc ttttccttta cgctaatatt ccaaatgcag ccataaccat
    25921 cgcaaaaaca ataacgagta gaatcaaaat ggtcatactg atttttttct tactgagtag
    25981 ataatatgct gcagcagtaa taattactgg aattaatgtt ggtaaaattt tgtccaacat
    26041 tggttggatt ttcatactaa cttcaccaaa agtgaattta agtggcgttg aaacactgat
    26101 aactgaagca attaatgatc caactactgt tagccccaat actgaagcag cttcagttaa
    26161 ataattaatc ttttcaccgt attcagtaac aatcttcatt ccctgcttat aaccaaattc
    26221 aaagcctctc attcggaaaa agaagattac catgattaaa atgaaccata aaaagattcc
    26281 tacaggatta ttttctaaag ccatataagc cgcaattgat cccatgatgg ttgggatcat
    26341 cgtcatcaaa agagagtccc caataccagc aattggaccc atcgtaccaa ctttaaagtc
    26401 attaatcgca tcttttgctt ctaatccttg cttttcctcc attgcaacac aagcacctaa
    26461 aataatggct gccagccaag gctgtgtatt gtaatactga aagtgattat ttaaagcttc
    26521 gatgtattca tcatcatctt tgtataattt tcttaaaata ggtgacaatg cgtaagtcac
    26581 agatgcacct tgctgtgttt gataattaaa ggtacaaact gacatcaacc aacgatagtt
    26641 cgctttccta agatcctttt tcgttacttg atatttttcg ctcattttat tcatcctccc
    26701 caataaaccc aacatcagaa gctcccgcag aagcagtgat ttgtgcatct ttttcagctc
    26761 tatgatacac attaaatgct aaagcgaatc ctatgatcgc cgctccaata ataggtacct
    26821 tcaaaaaagc ggctaagaaa aatccaacaa gtaaataact aatgtatcga cctgtaggca
    26881 taaagcgtaa caacatcgca ataccaacag caggcaacat ttttccggct aatgttagcc
    26941 catccataaa ccaattaggt aaaacatcca aaacgtaatt aacagcagac tgtcctaaag
    27001 taacggcaat aaatactgga attgctgcag ataaaccaaa gaaaattggc cccaaccaca
    27061 aaacactatt cattttctta aattctcgtt tattcgcata ttcctgtgat ttgcgaacaa
    27121 caaaaccatt taagattttg acaattacat ctagttgcac ccctaacata cctacaggta
    27181 gtcctattgc taatccggct tctactccaa gctggctacc aaatgccgtt gcaataattg
    27241 cgcacgttcc atagtccggt acagatgagc caccaaaacc ggcaactcct aatgacatca
    27301 attgaactgt accgccaact atcaaacctg tttttatatc acccatcaaa attcctgtga
    27361 ataatcccca aaaaactgcg taatttacaa agatcatcgg aatcccataa taatcaacat
    27421 gtttaataaa tgctaatata gtcagcataa taatttgaaa aatagtaaaa tgcattatcc
    27481 ttactccttt atcctaattt tgattgaatt tcctgtgcct tatccgccgg aacaaattgt
    27541 gtaacaattt ctacaccaga atccaacaat tttttatacg tgctgatatt ttcatctgat
    27601 gcataggctc tagaagatag ctgtaatgca ttatctttaa caacagtacc gccaacaatt
    27661 agtcggtcaa ttctaatacc gttatctact aaatgaagga ttgtttccgg ctctttcacg
    27721 atgacaaata ctttttcctg atcgtatttc ccctttttaa aattcgcaat cgctgtttct
    27781 tcagtgataa ttgatacagc cattccagca ggtctagcta aacgcatact tgatttttgg
    27841 atgtcgttat ttgcgacaga atcatcgatc accatcactc tgtttgcttt agacattggt
    27901 gcccattgcg tgacaataat cccatgcaat aaacggttat caactctcga cataacaata
    27961 ctcataaata cttcctcctt tatctttcat tttcttacaa gcaattacca tgccaaaaca
    28021 aaaagaactt gataaatcaa gctcttttta cataatcata aacgtattta acctcatgaa
    28081 tcgggatgtc gacactataa taatgttcaa cgacactgaa agattgctta aaatgtgtct
    28141 gaaatttttg aaattcctca ctcgtttcat tgaaataaat ttcatcttcc aaagtatttt
    28201 gttttactaa tcgttcaatt aagcaactaa tgtgaacata taatccgaaa cacgtattat
    28261 tatcaaatga aattcctaga tcattttgta agatgtatat agcatctgaa acccgttcta
    28321 ataatttagt cggatttaat atggtcaatt gctccattaa attcgttaga ctaaaattct
    28381 tcagaatatt attgttcaaa actttcagtt cattatcatt aaagtgtcta ttcaattgat
    28441 tggagaattt acttatattc attcctaaaa tcatatcttc aattgaaaag aattctacat
    28501 cttctagatg tggatttagt gttccaacaa taaaaagaat attgttctct ttacgaagct
    28561 ccgataaata gttttcattc actaaagagt aatagtcaca cgtttctatg acaagatcag
    28621 tatcccttgg aaagctatct tcaagtatct tttttaattt aaacgaagtt cctaatccgc
    28681 ttgcacaaga acagacaatc atatccttct taatatttga tgattcataa atataatcca
    28741 cattttgatt ttcttttact tgttcaagaa tctgtttaag cggtaaatct aataatatct
    28801 gatttgcaac atcaatagcc attcttgttg aaacattgtt tagtagtccg aatttttttt
    28861 gagcgtctag ttccaaattt agataaattt tctccagtga acccatatca acaagtaaaa
    28921 taatttcttc atatccggta cactcttgta agtatctatt aattttgtct gcaatctcac
    28981 tacttgaagt cattagtggc atatcaatgg cgtcgtatat atatctctca acgatcctat
    29041 tagcagtact tgctatacag gttgccgtta gatctccatg agccaaaatc agtgctaaac
    29101 gtttttgctg aaattcttta ctacacatta acttaaataa aataaccaaa acattttttt
    29161 gaatttttgt taaataaata tcccctattt gaataaaacg attgactaat tgaacttctc
    29221 gactacacca actattaata tcattgatga aggaatataa tttatcgtcc gatgttaatt
    29281 ggatttgata aatccaagaa ttcaaaaaga tagacgttat ttgaagataa taatagtcaa
    29341 attctatttg aaaatctctc tctatttcct taacatactg tctaaatcga atctcttgat
    29401 aggtgggata tgttaaagct gaattatttt ggatatcaac attcttttgt ctaaaatatc
    29461 tatcatattt ctcaaaaagc tgctgcttat cagtgtcttt cttccacgtt tgataaagat
    29521 actctataag ttcgttgata ttaaaaactt cgtctacttt ttccaaatca tccatttgaa
    29581 tatattttaa ttcattctgt cttttcataa ccttcatgcc gttgaaattc acccattgtg
    29641 gtaaatgata catctgaata taaagttctt ccaaatcatc taagaaataa aaggcattgg
    29701 cacaggtcaa ttgaatgata tttttcaaat tgccaacatt ttcactaaag cgatagttta
    29761 ataaaaaatc taataattgt tgagatatca gtattttctt tgctagtctt tcttcttctg
    29821 atttaaaaaa tatcttgatt aactgttttc tttcataaag agaacgactg tctaatgttg
    29881 gaagtgttag ctttacaggt attcttctca acaaagttga taatagttta tcctcaggtg
    29941 gttctgatgt tgcaaatata atccagcaac ttgatgtgta ccagttttca ttatcaccca
    30001 tccgatgaaa tttcctcgta tccataaact gaaaaagttt ctcctgacat tcattgctca
    30061 atgcatgaat ttcatctaaa aataagactc cattattagc taattctgct aaaccctttc
    30121 ttccatcttc ggctcctgta tatgcacctt ttacatgccc aaaaaaattg gacaagaaaa
    30181 gttctggatt attggcatat tcactacaat taactgaaat gaaagatgcc ttctcttcta
    30241 tataattatt ggccaacaaa tccttataca cataatttgc taaatagctt tttccagttc
    30301 cagtctcgcc atgaagtaaa atgggtaagc catttggcgg atagtagatt gctgctttaa
    30361 tttgagcaat tacatttcgc agacttcctt gacttccgat aaccctttct attccaaccc
    30421 taaaagagct atcataccat ttttccgctt cctctgaaga gctaaaaatt tgttgatttg
    30481 gaactttctc cggtaaagaa aagaagatta ctggacgagt attgattttt ccaaattctc
    30541 catcttttac gaattcattc aaatattgag agacaatatt acgactaaca tttaattttt
    30601 cagatatttt ctgtgctgta ttgttccccc atttattttt aatctcagag ttctctatga
    30661 cataattgac aatgcgttct tttaattctt ccatcacagc acctcctcga cactattcga
    30721 attttgccga cagtattttt ccataaatat gacttgctaa aaaggctttg tttagatcat
    30781 tcaattggaa cgttgcttgt tttgcttgat ccgctaagac gttgaaaaga tactctggcg
    30841 aaatcgcata ccccaaaata tcaacgattg ttgcaatcag atcttctttt gattcttcat
    30901 ctgaaagtaa ttcacgatac aacatcgttt gtttgcttac tgtgtcatat tcctctaagg
    30961 attcatctgc taaaagaacc acttgttcca acaaacggtc ggataaatat ttatagaaaa
    31021 tcagccccaa taaataattt ttatattctg acgcatccat tttactacgg agattatctg
    31081 ccgcactaaa taacttctga tttaattctg cacccaaaaa gatacactcc tatctcttat
    31141 ttaaagttgc tatataccct cttattttag catactcagt tttagaaaaa gcaaaaaaat
    31201 aaagaaactt attcttgata acaagctacg tgacgtatat ccctaacaca aaaatagcca
    31261 tatcctttgg tttggatacg gctatttgat attactcatt caattatcaa atcacagtag
    31321 gtgtgaatct catcgggatc ggcaatatct acttctgatt aggatcaaag gtgattttct
    31381 caataaaggt gtgatacaaa gctttaactt gcttcttctc actctggata agaaagcaat
    31441 ccaaactagt caatatctgt tggacatctt tgacttgaat cggttcccct tgttgttgaa
    31501 agatccctaa gatttcattc tctcgaattt gtaactgtct tcgtttttca gtcaattcat
    31561 ttaaacgatc ttttaacatc ggaaacaatt cgggactatc ttccaaagca gcttcctgtt
    31621 tttgctctaa tagttagatt ttgcgtacca ttttcttctt ttattttcct tcgattgctg
    31681 attttaatat agtctaaagg aaatttatat ttattgtatt ctttataact tcaagagaac
    31741 ttcgtttgag tattactgta tttttttaat tccataagaa aattagttat tacctgccac
    31801 ctaatcagca aatctgaacc tgagtcaata taattaatat tattttccat ctccaacatt
    31861 gtaagtactg ttggtgttaa tatagggact ttgtacaatt gtattttttt gattttatca
    31921 tctgcacaaa ggagattttt taaatctaat ccttctttta tttcaaaagt tgaatttttt
    31981 aaatctataa gagtaaaacc aattccttca tactgaattt ctgaaaatcg aatttttctc
    32041 ccagttgata gatcaagggg cagaaaacct tttattacat tgtgagtgtc accatcattt
    32101 acaatgtcat taggaatttt gattttagaa ttctgtggag cacctattgg aagataataa
    32161 ttcattataa ttttgtagtc ttcatgagga aataaagtac ttttaggatt gctaagatat
    32221 tctcgtaccg aatctaagaa tggcttatcc attgaatctt catccccatt taaactaaaa
    32281 aacattccta caattgcttt tgcaactcta agtgaattca ttttctttat tgaatgtgtt
    32341 aaatcagact taccattctt taaacttgta ttgttataaa ggaaattttt attgtcttct
    32401 gcaaatctaa tatattcctc aacatactca ctaccaaaaa aactattaca tttactacat
    32461 aacgtattgg aatggcaaca gtttttttga caaattttat aaggtgcaga acttctttcc
    32521 gtatgctatt ccgattgtcc ttgacaatga gcctcctcgg atgtgacgat cttcggcagg
    32581 agctaacagt taaagttaga ctgcctcgct agcgttagca catccgagct cattatcaag
    32641 gattcgctgc gccaat
//
LOCUS       DARBDV010000021        32500 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000021 DARBDV010000000
VERSION     DARBDV010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.021"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(224..370)
                     /locus_tag="SCM56_000958"
     CDS             complement(224..370)
                     /locus_tag="SCM56_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HEG1109775.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            complement(474..785)
                     /locus_tag="SCM56_000959"
     CDS             complement(474..785)
                     /locus_tag="SCM56_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEG1109776.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(787..984)
                     /locus_tag="SCM56_000960"
     CDS             complement(787..984)
                     /locus_tag="SCM56_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HEG1109777.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1378..3105)
                     /locus_tag="SCM56_000961"
     CDS             complement(1378..3105)
                     /locus_tag="SCM56_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HEG1109778.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(3098..4291)
                     /locus_tag="SCM56_000962"
     CDS             complement(3098..4291)
                     /locus_tag="SCM56_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEG1109779.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4478..5122)
                     /locus_tag="SCM56_000963"
     CDS             complement(4478..5122)
                     /locus_tag="SCM56_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1109780.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(5216..5350)
                     /locus_tag="SCM56_000964"
     CDS             complement(5216..5350)
                     /locus_tag="SCM56_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HEG1109781.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5352..7484)
                     /gene="feoB"
                     /locus_tag="SCM56_000965"
     CDS             complement(5352..7484)
                     /gene="feoB"
                     /locus_tag="SCM56_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HEG1109782.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7481..7954)
                     /locus_tag="SCM56_000966"
     CDS             complement(7481..7954)
                     /locus_tag="SCM56_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HEG1109783.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8355..8579
                     /gene="nrdH"
                     /locus_tag="SCM56_000967"
     CDS             8355..8579
                     /gene="nrdH"
                     /locus_tag="SCM56_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEG1109784.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8738..10897
                     /gene="nrdE"
                     /locus_tag="SCM56_000968"
     CDS             8738..10897
                     /gene="nrdE"
                     /locus_tag="SCM56_000968"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEG1109785.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10947..11912
                     /gene="nrdF"
                     /locus_tag="SCM56_000969"
     CDS             10947..11912
                     /gene="nrdF"
                     /locus_tag="SCM56_000969"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEG1109786.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(12001..13140)
                     /locus_tag="SCM56_000970"
     CDS             complement(12001..13140)
                     /locus_tag="SCM56_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEG1109787.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13449..14162)
                     /locus_tag="SCM56_000971"
     CDS             complement(13449..14162)
                     /locus_tag="SCM56_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEG1109788.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14416..15117)
                     /gene="nagB"
                     /locus_tag="SCM56_000972"
     CDS             complement(14416..15117)
                     /gene="nagB"
                     /locus_tag="SCM56_000972"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEG1109789.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15351..16883
                     /locus_tag="SCM56_000973"
     CDS             15351..16883
                     /locus_tag="SCM56_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEG1109790.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(17074..18276)
                     /locus_tag="SCM56_000974"
     CDS             complement(17074..18276)
                     /locus_tag="SCM56_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1109791.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(18358..19047)
                     /locus_tag="SCM56_000975"
     CDS             complement(18358..19047)
                     /locus_tag="SCM56_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEG1109792.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(19153..19521)
                     /gene="rplL"
                     /locus_tag="SCM56_000976"
     CDS             complement(19153..19521)
                     /gene="rplL"
                     /locus_tag="SCM56_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEG1109793.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(19574..20077)
                     /locus_tag="SCM56_000977"
     CDS             complement(19574..20077)
                     /locus_tag="SCM56_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEG1109794.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(20281..20408)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(20487..21176)
                     /gene="rplA"
                     /locus_tag="SCM56_000978"
     CDS             complement(20487..21176)
                     /gene="rplA"
                     /locus_tag="SCM56_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEG1109795.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(21280..21702)
                     /gene="rplK"
                     /locus_tag="SCM56_000979"
     CDS             complement(21280..21702)
                     /gene="rplK"
                     /locus_tag="SCM56_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEG1109796.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            22045..22713
                     /gene="sdaAB"
                     /locus_tag="SCM56_000980"
     CDS             22045..22713
                     /gene="sdaAB"
                     /locus_tag="SCM56_000980"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEG1109797.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            22726..23598
                     /gene="sdaAA"
                     /locus_tag="SCM56_000981"
     CDS             22726..23598
                     /gene="sdaAA"
                     /locus_tag="SCM56_000981"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEG1109798.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(23689..24708)
                     /locus_tag="SCM56_000982"
     CDS             complement(23689..24708)
                     /locus_tag="SCM56_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEG1109799.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(24881..25786)
                     /locus_tag="SCM56_000983"
     CDS             complement(24881..25786)
                     /locus_tag="SCM56_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEG1109800.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(25845..26390)
                     /gene="nusG"
                     /locus_tag="SCM56_000984"
     CDS             complement(25845..26390)
                     /gene="nusG"
                     /locus_tag="SCM56_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEG1109801.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(26491..26661)
                     /gene="secE"
                     /locus_tag="SCM56_000985"
     CDS             complement(26491..26661)
                     /gene="secE"
                     /locus_tag="SCM56_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEG1109802.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(26685..26837)
                     /gene="rpmG"
                     /locus_tag="SCM56_000986"
     CDS             complement(26685..26837)
                     /gene="rpmG"
                     /locus_tag="SCM56_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEG1109803.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            26999..27643
                     /locus_tag="SCM56_000987"
     CDS             26999..27643
                     /locus_tag="SCM56_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEG1109804.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(27699..28202)
                     /locus_tag="SCM56_000988"
     CDS             complement(27699..28202)
                     /locus_tag="SCM56_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEG1109805.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(28215..28261)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            28799..29764
                     /locus_tag="SCM56_000989"
     CDS             28799..29764
                     /locus_tag="SCM56_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEG1109806.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(29863..31035)
                     /locus_tag="SCM56_000990"
     CDS             complement(29863..31035)
                     /locus_tag="SCM56_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1109807.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(31022..31147)
                     /locus_tag="SCM56_000991"
                     /pseudo
     CDS             complement(31022..31147)
                     /locus_tag="SCM56_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            31255..32334
                     /locus_tag="SCM56_000992"
     CDS             31255..32334
                     /locus_tag="SCM56_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG1109808.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
BASE COUNT         9946 a         6685 c         5796 g        10073 t
ORIGIN      
        1 ttataggtat agctgcggaa acatcagtaa ataattcaac aaaaaaagtg agtggtatca
       61 ataaacatac aaaaagatat tttcttttgt taagatcagt attagtcata taaaataaga
      121 gaaaaaagaa aaagtttccc acgaacattc ttaatagata agcttttgta tcgatagtaa
      181 tcaagagatc ccctcctgat tttaggatat cataaaaaaa attttagtta cactttttaa
      241 ataatgaaaa aacacattcc actaaatttg atgccggttt tccttgaggt tttgttccag
      301 cacctccttt aattttttta agctcttgat ctgaacattg tttagcattt agtctatttt
      361 tttcttccat tacgattccc cctacaaact ataaatactt ttatgtcact gattcaatat
      421 acctattttt ctaatcaaaa aattttgaga catatttaat attataagta tcattaaaat
      481 tgagatttat ctccataatc tgctcgatat aaaccattta ttccagcagt tcttccaatt
      541 tcactcaaat ttcttattaa ttcctcttga tattcagtga atcttatttt attagtataa
      601 gcagtaaaat atagataatt caccaatcta tttattaaag gaccgggatc taattcatat
      661 ttttgttttt ctaattttaa atataccttt tgtaaaacct caagaatatc agaatattta
      721 ggatctttac ttatagatat atcattatat aattcatgaa caatttgctt agcatttttt
      781 ttcattttag cacttccctg gaattgctcc acctaaaaaa ccacctatag acattcctgc
      841 aatacaagta gttgccttgg cccaatcgac cgtacattta tttttagtgc aatacactcc
      901 atttccataa tattttccac tatgagtggt acccccatag ataagttgtg tctctttaat
      961 agacaaaatt tttaaatgtt tcataatcta atcaccccta aattaatgtt tttagactat
     1021 tattctaaca ttcattaaat ttcaaaaaac aattccttct tgaaacgtag caaaataatt
     1081 cctgaataag aatttaatca cttttaagta tataaactat gtatattctt taatattttt
     1141 tgagaatgca cttcgtacat aataaacttt aatcacatta gtctttcata cctacgatcg
     1201 atctctttga ctcgcaccca taaattcata atagtttgtc ggcgtctttg gacgtttcga
     1261 agatgagcta cgcattctct ttttttatag acaaaaaaga tagagacgaa catggagctt
     1321 ccaaatttgg atatttgatc caattttgga agctccattt atttcgtgtc tgtcttttta
     1381 ttttttccag ctttcgacta attcttggtt ctctttgatc cagtcttttg ctgcttgctg
     1441 tggatctttt ccattgttga tttccaacat aactttttcc atatcttttt ctgaccaatg
     1501 gaaattgtct aatactttgt aagcttctgg ctggtcttct ttcaatcctt ttcgaaccat
     1561 cgtatggatc tgctcttcgc ttcccattcc gttttcagga tcttctaagt atttcagatc
     1621 atatttggca aacatccaat gaggtgtcca tcctgtaact acaattggtt cgtgtttttt
     1681 gatcgcttga cctagtgcaa cggtcattgc tcctgaagaa gatgtctcta ctttccagtc
     1741 tttcaagttg tcatattttt ggatcgtgtt ttcagcagcc gtaactacac cagctcctgg
     1801 ctcgattcct atgattttct tacctgcctg atcggttaac tcatcgatcg aatttacatc
     1861 catgtaggct ggcactgcta aaccaacttt tgctcctgtc aaattggcac ctaggtcttc
     1921 gacttgatct ttatattgtg catattgtga accatgagtt ttaggtagcc aagctgaaac
     1981 catggcatct gactcgccat ttgaaacaga tttccacatg attgaattat ctaatggcgt
     2041 catcgttaca tcatagccca tttgtttcaa tacttcaccg actacatttg ttgaagcaac
     2101 ttctgtatcc cattcaacat aagataagtt gatacgtttt gtttctttgg cagatgaaaa
     2161 actccctata ccaatcgcac ctaagatcac tactactaca gcaccaatga tcagtccttg
     2221 acgtttttta cttttctgtg ttgttgctcc tgcagctttt ttattgtttg gctggttcaa
     2281 atgctgtgta aaacggtcaa tgatgattgc taagataact aatgcaaccc cgttgacaaa
     2341 tccattcccg acttgtgctc tttgcaatgc tgataatacg ccgcgtccta agccgggtgc
     2401 accgatcata gaggcaataa cgaccattga aagtgacagc attgttgttt gattgatccc
     2461 tgccatgatc gtacttttag ctaaaggcaa ttctagtttg aatagttttt gacgacttgt
     2521 gctcccgaat gaatcagaag cttctactaa ttctttagga acttgtcgaa tccctaaatt
     2581 cgtaaaacga acggttggtg gcagagcaaa gatgactgag gcaaataccc ctggaaccat
     2641 cccgataccg aaaaatgcaa ctgctggaat caaataaacg aatcctggca ttgtctgcat
     2701 aaagtcaagg atcggtttga taatactttg ggctttactg ctttttgcca tcaagatccc
     2761 taatggtacc ccaatgatga tcgagatcac actagataaa agaacaagtg tgacagtatt
     2821 catcaaatca ttccatagac cttggttata aataaaccat agaccgatta atgtgaacaa
     2881 cgtaagtcct ggacgtttct tagaaataaa gaaagctgct accgtcaata acaagataaa
     2941 caaaggagct ggaatcacta acagtaaatt tgtaatcccc gacatcactg cctgtccaat
     3001 cgtttgtaaa aatgaaaata ggccagaaaa agttgtcgta aaccattctg tgatattttc
     3061 tacccagttt gccactggta attgatagtt caatagatta ttcatgttca ctcacctcgc
     3121 ttgtttctgc taatgcttca atcacactac ctcggatgac gacacctttt aattttccat
     3181 cctctacaac tgcaagtgga gtcggtgagt cataaataat attgaaaata tcacttacca
     3241 ccatgtcttt cccaatcacc gtcacatttt catctaggaa gtcgatcaat ggacgttttt
     3301 gcttcctagc ttctaacgct tgatctgcag tgatgatccc tttcaggtgt ctctttctgt
     3361 caacagccat cagcatgctg acttcttcat tacgcatcct tgtcaatgca acgtttggtc
     3421 catcagattc gatattcgtc gtcaatgcag gtaccatgat gttttgagcc gtaagcactt
     3481 tggaacgatc gacttcttcc acaaattcac gtacataatc atttgctgga ttggtcaata
     3541 tttcttcacc tgttccgatc tgcatgattt cgccgtcttt catcaaggcg attctgtccc
     3601 caatccgtaa agcttcattc aaatcatggg taatgaaaat aatcgttttt tgaacattgg
     3661 cttgcaggtc cagtaattcg tcttgcattt ctcgccggat cagcggatca agagccgaga
     3721 acgcttcgtc catcagcaag atctcaggat catttgctaa agctctggct aggccaaccc
     3781 gttgttgcat cccaccagat aactgactag gatattggtc tttaaatgaa agcaaactag
     3841 aattttctaa tgctttttct gctttttctt gtcgttcttc tttaggtaca ccacggactt
     3901 ctaacccgta ttctgtattt tccaagatcg ttcgatgtgg aaacagaccg aagttttgga
     3961 aaaccatatt gattttatga cgacggactt ctcgcaattc ttctttgctt aacttcgata
     4021 catcttctcc atcaatatag atactgcccg aagttggctc aatcagacga ttcaatagac
     4081 ggatcaatgt ggatttccca ctcccggaaa gccccatgat cacgaagatt tctccttctt
     4141 ttacgtcaaa attcacgtca tacacaccaa ccgttgcacc ggttttcttt aaaatctccg
     4201 ttttcggctg gtgatcattc atcatatcta aagcggcttg tgtcttttta ccaaatatct
     4261 ttgttaaatg ttttacttct acttttacca aacaaattct ccttttttat gtaaattcat
     4321 ttttctttct gaaaaaagtg accacactat gttaacatca tgtagtcact ttgtcaaaaa
     4381 tgaaagaaat ttgcttgtcc aaactgaaaa aaacttggct aaatctttgt aaataaaagg
     4441 tttagccaag ttaatatttt tttaatattt cacacaatca ttctgcaagc tgatgctccg
     4501 ggtaaaaaaa gtttgtcata cgctgtagtg tatattcatt tccaataaaa tgaacgatgt
     4561 cccctgccgt aaatttagca taggggccag gtgaaacaag cagttcttca ttatgagaaa
     4621 ttgcgataac tgtggcgcca gtcgcctgcc agatattcaa ctcgctaatt gaacggtcca
     4681 gatgcattgc tttttctgat aaagataatt caaacggaag taaaggattc atcttatgta
     4741 ttcgcttcgt ttgattgacc agctgatcca ataattgaga aaaatgattc agctcatctt
     4801 gttgcttctc cacacttgcc atgatttctg cttttgtatc ttggatggat tgtacatctt
     4861 gatattgcaa taggaaatct tttgcttttt ccttcgaagc aacaaatgcc ccacttccat
     4921 gccgtacttc catgattcca agatctacca gcacattgat agccttgcgg gtagtctcag
     4981 gagaagcatt gtacgtactg gcaagtgttg agcgggcatg gatcttctca cccactttat
     5041 atcggttttc aacgatacgt tcggctaaat caacagcaat ctgctggtag cgcgggcgga
     5101 cgattttttt ctttccagac attttctttc ctctttcttt ttttactttc cctcatctta
     5161 ctatgctcta gcaaaataag caaaaaaggg gagaatagcc tcttgcaacg actgatcatc
     5221 gcttgatctg ttcctttttt actgggcaag ctgtttgaca gtcatcacaa ttcttaccgg
     5281 attttaaacg agtatagaca attgcgcctg cacttccaaa gattaacaca cttaataaga
     5341 atgtcgccat cctattcctc tccttctaat gacaatatag tataaaactg gttcttggat
     5401 tttggctgac ggaacaaaac ataaacgagt cctatcatca gtaaaatagc aagatacgtc
     5461 cctaacgcta cctgtccacc ctccaataag tgtcccaact gataaatcac aaaactaact
     5521 aaataagcta aaccgcattg atagcctatg gcgatccaag tccatttctt ggcattcatt
     5581 tcccgatgga tagccccaat tgccgcaaaa caaggcgcac aaagtaaatt gaaggctaac
     5641 aatgaataag ctgctgcggg agtatatgtg tgttgtagca cttgccagat ttctctgcca
     5701 ttttcggata cttccgcatg accaaacaaa ataccaaatg tgccaataat attttcttta
     5761 gcgaccaatc ctgtaatcgc tgcgactgtt ccttgccagt ttccccaacc aagcggctga
     5821 aaaacaactg ccaggattcg cccgaaaaag gctaagatac tttggtcttc agaaacaggt
     5881 tgagcataaa aattataaga agagctgaac cagatcacga tactagaaac aaagataatc
     5941 gttcctgctt ttttaacaaa agacttgctg cggtcatagg tttgtcttag gaccgctgac
     6001 cattgaggaa aatgatatgc gggcaattcc atgataaaag gagctgtatc tccagcaaac
     6061 aaacgtgttt ttttcaagct gattccagag aaaataattg cagtcatacc aagaaaatag
     6121 gcagaaggag ctacccaaga ttgatgagga aaaaaggccc ctgctactaa ggcaataatc
     6181 ggcaactttg cagaacaagg catgaaggtt gtgaccatga tcgtcaaacg acgatcttgc
     6241 tcgttttcaa tcgtgcgact ggccatgacg cctggcactc cacatcctgt agcaatcagc
     6301 ataggaatga aggattttcc tgaaagaccg aatttcctga aaagtcgatc catgacaaaa
     6361 gcgatcctcg ccatatatcc gcagtcttcc agaaagctca agcataaaaa caatataatc
     6421 aattgcggta gaaatcctaa cactgctccc acgcctgcga tgatgccgtt caagatcagc
     6481 tcctgcatcc atcccgctac ttgccattca gctaacaatc tgccaacatg ttctggtaac
     6541 catgtaccaa acaatgtgtc attgatccaa tctgtcccca ttgttcctac tgtttgaata
     6601 gccagataat agatcagcca catcacaaat gcgaagatcg gcaaggctaa ccaacgattc
     6661 gttgcaatct gatcgatttt gtcactcatc gataattgga aagaatcatt tgacacaaca
     6721 cagagtgtac atagttgggt aataaattca taacgttcat tgaccagaat cgtctcacta
     6781 tcgtcgtcca atagtttctc tgttaactgg atcagttgct cgatctcttt ttgctgagaa
     6841 actgctatct cgtaatcctc tcttaccttc tgatctcgtt caaaatattt cattgcatac
     6901 cagcgtttct tgccgctttg ttctcctagt acatcttcaa tttcatgtaa agctgcttct
     6961 aaacgtggat catatgtaca gggaacagga atagaaggcc gcttttttgc agactcctga
     7021 atcttttcga ttagtttttc gattccttgc tttttcaata cgctgattgg taaaactggt
     7081 acaccaagac cataggaaag cttttctaga ttgatttttt ttccttcttt tttgatgaca
     7141 tccatcatat tcagaccaat gatcatagga atgtctaaat ccaataactg cgtagttaaa
     7201 taaagatttc gttctaaatt ggtagcatca ataatgttga tgatcagttc aggctgttcc
     7261 tgaaccaaat aatctcttgc aatcacttct tctggtgtat aaggagacaa agaatagatg
     7321 ccgggtaggt cctgaatatg aatgttcgga ttttttttca acgatccttc ttttttttct
     7381 actgttacgc ccggccaatt ccctacatat tgattcgaac cagtcaagag attgaacaag
     7441 ctagttttcc cgctgtttgg atttccgact aacgcgatat tcattctttt tcctcccttt
     7501 gtatcaagat tagttcggcc tctgactttc gtaacgtcag ttcataccct cgcactgtca
     7561 gttcgattgg atctcccaga ggggctagtt ttcggataac tatttttaca tttcgcgtca
     7621 atcccatatc cattaaccgt cgcttcactg cgccttgtcc gtggattgct acgacgatcc
     7681 cagtctctcc tgcttttaat tgatcaagag aagtccagct ttcagaggaa gcagtctttt
     7741 tattgacaaa gatttgacct agtatttccc gacttactgc cactcgatga ctttttacca
     7801 gtaaaatggc attgttgcct gataaattga cgacgacgat tttacttccg gcaacgatcc
     7861 cgatattttg caaatggcgt tttgtttttt cttctgtttc gattcgttcg acttgataaa
     7921 cgaaattctg ctttgcctgc gctaaggtct gcatattgat tcctctcttg tgaaataaat
     7981 catgtattaa aatcattcat tatatgttct aattttctta cgtgttacgc aaataaatga
     8041 gaatcattct cacatagaat actctcggta attgagaatg tcaattatac ttgtgatatt
     8101 atgagcgaat ctttagtatt gatagataaa aatgagaaaa aaatatttct tcatagtttt
     8161 ccacaatttt ttctctatta aatttgctat tttcccacac gatacttgtt ttcagctctt
     8221 ttttcaaaaa cacaaaactt agtattccac agtttccaca acacaatatg tagtgtttgt
     8281 tgtttgactt ttttactaag agttttatac tatctattat agataataag aatgtgttag
     8341 aggaggcact caccatgaac gtaaaaatct tttctaaaaa taattgtatg caatgtaaga
     8401 tggtcaaacg gtttttaaca gaaaatcaaa ttgcttttga agaagtcaat atcgatgaac
     8461 agcctgaagc agtagactgg ttgaaagcac aaggattcca aagtgttcct gtcattactt
     8521 ctgatgcaga aacagttatc ggattccgtc ctgatcagtt gcgtaaactt gccagctaac
     8581 atgactaaaa gaatcaaaac gtgatacatg gatttgaagg gcttgagaca gcaccagttt
     8641 ttgtcgcaaa cttccaaatc cttttttaaa gcttaaaaac aatgcaatga tttgcaatca
     8701 agcatcatca aatacgaaat gaaggttggg cgtacgtatg agtcttaaaa atttacaaga
     8761 tgtcagttat ttcaagctga ataatgaaat caatcggcca gtcaatggtc aaatcccttt
     8821 gaataaggat aaagaagctt tagttgcctt ttttgaggaa aatgtcaaac caaatacgat
     8881 gacttttcta tctattatgg aaaaaatcaa ttacttgatc gatcaagaat atatagaagc
     8941 agaattcatt caattgtatt caccatcatt tattgaaaag ctatatcaat ttttggacga
     9001 gcaacatttt tctttcaaat cttttatggc tgcttacaaa ttttattctc aatatgcgtt
     9061 gaaaaccaac gacggttccg cttatctgga aacttatgaa gatcgagtag ccttcaatgc
     9121 gctttacttt gcgaacgggg atgaagaatt agcacttgct cttgcagacg aaatgatcca
     9181 tcaacgatat cagccagcta caccttcttt cttgaatgct ggacgtaaac gtcggggcga
     9241 attggtgtct tgtttcttga ctcaagtcac tgacgatatg aactctatcg ggcgctcaat
     9301 caactctgct ttgcaattgt cccgtattgg tggcggcgta gggattactt tatcaaatct
     9361 tcgtgaagcc ggcgctccta tcaaaggata tgagggtgct gcaagtggtg tcgttccagt
     9421 catgaaatta ttcgaagata gtttcagcta ttctaatcaa ttaggtcagc gtcaaggagc
     9481 tggcgtggtt tatttgaacg ttttccatcc agacattatt gctttccttt cagcaaaaaa
     9541 agaaaatgca gatgaaaaga ttcgcgtaaa aactctttct cttggcgtga tcgtacctga
     9601 taaattttat gaattgacac gtaaaaacga agatatgtat ttatttagtc catatagtgt
     9661 cgaaaaagaa tatggtgtcc cattctctta tgtaaatatt acagctgagt atgatcatct
     9721 agttgcaaat ccaaacattc gcaaacaaaa aatcaaagcg cgtgatcttg aaaatgaaat
     9781 ctcaaaatta caacaagaat ctggctatcc ttacgtaatc aatattgata cagcgaaccg
     9841 cgagaatcca atcgatggca agatcatcat gagtaattta tgttcagaga ttttacaagt
     9901 tcaaacgcct tctgtgatta acggcaaaca ggaatatgaa gtattaggaa cggatatctc
     9961 atgtaactta ggctcgacaa atatcgtgaa tttgatgcaa agccctaact ttggtcaatc
    10021 tgtgcgtacg atgactcgtg cattgacatt tattacagat gcatctgata tcgatgtcgt
    10081 accaccgatc caaaacggaa ataagctgaa tcatacgatt ggattaggtg ccatgggctt
    10141 acacacttat cttgcaaaag aacaaatgga atatggctca gaggattcat tagactttac
    10201 aaatatttac ttcatgctgt tgaattattg gacacttgtt gaaagcaaca atatcgctcg
    10261 tgaaagaaaa caagttttcc ataattttga aaaatcagct tatgctgacg gcacttattt
    10321 cgaaaaatac gtaactggag aattccagcc aaaatcagaa aaagtcaaag cattgtttga
    10381 aggcattttt atcccaactg ctgaagattg gaatgctttg aaacaagcag taatgaaaga
    10441 tggactgtat catcaaaatc gcttggcagt agcaccaaat ggctcaattt catatatcaa
    10501 cgatacgagt gcaagtattc atcctatcac tcgcatgatc gaagaacgcc aagagaagaa
    10561 aatcggtaaa atttattacc cagcgcctta tctatccaat gagacgattc cgtattacac
    10621 ttctgcttat gatatggaca tgcgtaaagt aatcgatgtt tatgcaatgg cacaacaaca
    10681 cgtcgatcaa ggcatgagtt tgactttatt catgcgttct gaaatcccag aaggcattta
    10741 tgaatggaag acaacaacga agcaaacgac acgtgatttg aatattttaa gacattacgc
    10801 tttccacaaa ggaatcaaat ccatttatta cgtacgtacc tttactgatg atgcagaaga
    10861 aatcggaagc aatcaatgtg aaagctgcgt gatctaatat catagataga ttcgcttatt
    10921 catattttaa ttaaaggagc attctcatgt cagaaacata ttatgaagca ataaactgga
    10981 atgaaattga agatatcatt gataaatcca cttgggagaa gctgacagag caattttggc
    11041 tagatacacg tatcccactt tcaaatgacc tagatgattg gcgtacattg tctcaattgg
    11101 aaaaagatac agtaggacat gtatttggcg gtttgacact gctagatact gtccaatctg
    11161 aaagtggcat ggatcagttg cgtaaagatg tccgcactcc ccatgaagaa gccgttttga
    11221 acaatattca attcatggaa tccgttcatg ccaaaagtta ttcttctatt tttagtacac
    11281 tgaatacgaa aaaagaaatc gaagaaattt ttgactggac aaatacgaat ccttatctgc
    11341 aaaaaaaagc tgagcgtatc aatgaaatct acaaacacgg tacaccgcta gaaaagaaaa
    11401 ttgccagcgt ctttttagaa acattcttgt tctattcagg tttttatact cctctttatt
    11461 atttaggaaa caacaaattg gctaacgtag cagagatcat caaactaatc atccgagatg
    11521 aatctgtaca tggaacctac atcggttaca aattccaatt aggattcaac gagttacctg
    11581 aagcagaaca agaaacgtta aaagactgga tgtacaatct tctttatgag ctttacgaaa
    11641 acgaagaacg ttatacagaa gaattatacg atccaatcgg ctggacagaa gaagtcaaga
    11701 cattcctacg ctataatgcc aacaaagcat tgatgaacct agggatggat ccactctttc
    11761 cagatactgc caatgatgtc aacccaatcg taatgaacgg gatttcaaca ggcacaagca
    11821 accatgactt cttctcacaa gtcggaaacg gctatttgtt aggtcatgta gaagcgatga
    11881 aagacgacga ttatttgatt ggattggaat aaggatttat tttaattcaa aaatttttcg
    11941 aaaaataaaa agagtttagc gtacagatat tttaaatgta cactgaactc ttttttatgc
    12001 ttattcactt gtttcttcca gcacttttgg actgtctaac ttttccgaaa catgttccat
    12061 tcttctcaat aaatagattt tccggatatt taatattatc gtcttaatca ctgcatagac
    12121 aggtatgcaa atcagcatgc ctaatattcc tgcgacactt cctgctccaa tcaataccaa
    12181 gatgatcgtc aaaggatgga tattcattga tttcccaaat aacaacggct tgatcaaatt
    12241 tccatctaat tgttggatca ctaaaatcga caacgccata tacaaagctt gaaatggaga
    12301 gataaacaaa ccgacaatga ttgctggtac cgctccaata aaagggccga catacgggat
    12361 aatattcgtg attccgcaaa tgaccgcaag caacaagcta taaggctgat cgaatattct
    12421 cattgccaaa aagctcatta caccaataat caaagcatct aagatcgtac tgctgatata
    12481 ggcagataat gtttcgttta attcttttac cgtttggcgt aactctttac gaatcgcttt
    12541 cggaaagaag tgagacaacg catcaataaa tttgtgccca tccttgaaca taaatagcaa
    12601 aataaatgga acggtaaata acaaaataaa aaattgcatc ataaatgaaa tgatttttga
    12661 gacacttgca gataaactga ctaatatgat attgaacaaa ttactaagag ataaatttgc
    12721 tgatgctaat tgttcttcca aattgaaatt tttaaactct tcccgctgcg ccaattcatt
    12781 taaccatttg cttgtttcat ctgcatacat tggcaagctc tgggtcaatt gaattgtctg
    12841 ttccattaac tgaggcacta cattcatgac agttaaaaca actaaaatga ttacgaccaa
    12901 aaacgtcaat aaaaaaccga acactctcgg gatccgatgc ttttcgagaa aaacaacgat
    12961 tggatcgaac atataataga taaaaccagc aacaagcaat ggtaaaaaaa ctgccgaaag
    13021 cattttcaat attggactaa aaatattcgg catttgtaaa ataaagtaca cgccgatgat
    13081 cgtgagtact agctccactg tccagaaaaa taattttgat tgtttaaaac gtgattgcat
    13141 acatctcctc caaggtcctt tgatacttct attctatcat ttaacaaaga gttacgcgct
    13201 gagattttca tttgcttaaa aaaaacaaaa aaaataaaca tattttaatg agtagacgag
    13261 tacttaaaca aaaacacggt ttggagtagt gacaaaaatt atactgcagt ccctttttcc
    13321 aaataaacgg tgttcaaaag cttgcatctt cgtaataagc ccagccaaac gaaaaatata
    13381 aggaactatt tttatttggc tgggcttatt acttgatgta ggacatctat tttacaacct
    13441 cagatcattt attgcgcaat actgactaaa tgcaactttg tgatattttt gtgtacggac
    13501 aactcctcga tcacatcggc ataagtgagc ccttttggaa gatcaatcgt atagatattt
    13561 ttgtacactc ggtagtcatc aacgaatttt acgtcaaaat tcacatcttc gattacgata
    13621 tttttctctt caaaataatc ggtaagaaaa tctttggttt cttttctatg gataaattgg
    13681 atttctaact ttttagtcgt aggtacatgg atcacgcgtt tcactactgt gagtgcaata
    13741 gcaatcccga taaaactgat gatcgcaatg gaatagtaac ccatgccgat ggcgatccct
    13801 aatccagcaa ttgcccataa agaagcggca gtcgtaagtc ccgtcaccga ttgtttcgtg
    13861 acgatgatcg ttcccgcgcc taagaaaccg acaccactca ccacttgagc aatcagcctt
    13921 gcttcatctg aacgaatcgt tccagcaagc tgtggatact ctaaagcatc tcttaatgca
    13981 ccactagcga tctcaacttg gatcaatgca atgatacatg ctcccataca aactaaaata
    14041 tgcgtacgca tacctgctgg acgattttta tattgtcgtt caaaaccgat tgctccgcca
    14101 aatagcatag cgatccctaa acgtaaaata atctctggaa ttgtcaatac catatcattc
    14161 atctaaacac cctccagtcc aatatatcat gtctgaaagg agcagacaaa aaaagtgcaa
    14221 tttttacctc ttacgaaaaa aggactgtga caaaattttt gtcatagtcc tttttatgaa
    14281 taaacggtgt tcaatcagct gaccctcggt attactcaac aaacggcaaa atatggggag
    14341 ctatttttcc tttgtcttct ctaatactca ggtcaaaacg ctgatttcac aacctcttta
    14401 gcctactatt gaaaattaca acttacttgc tgcttcttca tcgatgatca caacaacatt
    14461 gtcgtgattt tgcaatacac ttgctggcaa atgattcgtt acaggtcctt caatcatccc
    14521 tttgattgca tcagctttgc tttcgccaaa tgccatcaag acgatttctt tacttttcat
    14581 aattgaaccg attcccattg aaacagcttt tgttggtaca tcttctgctt tatcgaagaa
    14641 tcgtttgttt gcattgattg ttgattctgt taagtcaact actgaagtcg ttccgtcaaa
    14701 tgaagcacca ggttcattga aaccgatatg tccattttgc ccgattccta agatttggat
    14761 atcgattgga tgtgcatcga tgatttgatc atactttttg catgctgctt ccagatcttc
    14821 tgcttttcca tctggtacaa acgtttcttt gaatggtttt ttatcaaata attggacatc
    14881 catgaaatga cggtagcttt gttcatctga accgcctagt ccaacgtact catctaagtt
    14941 tacagaagtc atttcactga aatccaaatc gcttgaagtc atttctttat ataaagtaac
    15001 tggtgtgctt cctgttgcca atcctaatac ttttgcacct ttgttcatac cttctttgat
    15061 gatttcaaat gctttttttc cgccttcttc agcgtttttt acacgaatga tttccatagt
    15121 gattgctcct ttatattttg gtatagccca attatagcat tctttttttc tttttacaat
    15181 aattagtata gaccaaattg catttttttc attttcttga caataaatag gaatccgtgt
    15241 taggtttcat aagatggttc aatcaaacaa tccatgttgc atgaaaacca agcaagtgat
    15301 tcactgtgga cgctttatgt ctgcagtttt tttatgaaaa aggagttatt atgagtatac
    15361 tgacattaaa caacattacc cagcaatttg gcgacaaaat cctctatgaa ggtgtcgatc
    15421 tgcaattaaa tgctggcgaa catcttgggc tgattggaca aaacggagcc ggtaaaagta
    15481 cattgattaa aatcatcact ggtgagatct taccagatga cggacagatc cattggcaaa
    15541 aaaatattca caaaggttac ctcgatcaat atgtggaagt cgatgaaaca ttaacaatcg
    15601 aagaatttct aaaaactgcg tacgcaaaag aatacgaaaa ggaagaaaaa attgcttttc
    15661 tttaccaaac atatagcgag accttcgatg attccttgct ggaaaaagct gggaaactcc
    15721 aaacggaatt agaccaagga gtgttttatc aaatcgacac cttagttgct gagatgagca
    15781 gcgggttggg gatcgatgtc ttaggtttag attcactgtt gaaaaatcta agtggtggac
    15841 aacgttcaaa ggtgatttta gcaaaactac ttttagaaaa cccagatgtt ctgatcttag
    15901 atgaaccaac caatcactta gatgatcagc atatccaatg gctgacgcag tttcttcagg
    15961 attttgacgg tgcgtatatc gtgatttccc atgaccaaga atttcttgat cagatcacca
    16021 cccatatcgc agacatcgag tttggaaaga ttactaagta tactggccat ctaaaacaag
    16081 cattgaaaca aaaggaacaa aatcgcgaaa gctatttgcg ccaatatcat gctcaacaaa
    16141 aacaaatcga aaagaccgaa gcttatattc gaaaatacaa agccggttcc cgatcaacga
    16201 tggcaaaaag ccgacaaaaa caattagata aaatcgaacg gctgacgcct ccagcttcag
    16261 caagtaaagc tgtctttgat tttccattct cgcctattgt cacgacatta gcagtggaaa
    16321 caactgattt agtcatcgga tatgatcgac cgctacttgc acccatcaat ttgaccatcc
    16381 gctttggtga aaatattgcc attcgaggct tcaacgggat tggaaaatct actttattaa
    16441 aaacactgat cggtgagatt gaaaaaataa gtggcgattt ccacttccca gataacacaa
    16501 aaatcaatta cttttcacaa gatctgtact gggaaaatcc attagaaaca ccactacaat
    16561 atttaagcag tcaatttcca aaagcgacta tcaaagaatt acgtcgccaa ctagctaaag
    16621 cagggttagt taatcaatta gcaagtgaac ctctcactac attaagtggt ggggaacaaa
    16681 ctaaggtgaa acttgcacag ttgacaatga accaaggtaa tttactgatt ctcgatgaac
    16741 cgacgaacca tatcgatcaa gaaacgaaag aaagcttaca agacgggata cagaaatttc
    16801 caggaacagt aatcatcgtc tctcatgaac aagaatttta tcaagatctt gtgcaacgag
    16861 tcattgagat tgaaccaaaa taaacgtttg attgaatagt tgaaaaaaag gagctgtgac
    16921 aaaagttttg tcacaactcc ttcttctgaa taaatggtgg ccaattagct gaccttcggt
    16981 attagctcaa aaaacggcaa aatatgagga gctattttta tttggctatt cttattactt
    17041 gatgcagaac agcttttcac aacctctttt cgtctatttt ttattttcga gatttctctt
    17101 tcctttaaaa acaactgttt gaaagaagaa cgcacatcct tgaaaaacaa gcccgctaac
    17161 cgttacacct aaccagccat actgctgcca cattgtcgtc cctagcaatg aaccagccgc
    17221 acctccaata aagtacataa acataaatac agtattattt cggttgcttg ctgcttcacc
    17281 tagcccttgt actcgtgttt gattggcgac ttgaccaaat tgattcccga cgtctaataa
    17341 aataatagct aaaagcaaaa tggccaaatg gcttccgcca accaataaag caataaagct
    17401 tattgtttgc ataacaagtc ccatatacac gatttttctt tcagaataag aatctgccag
    17461 tcgtcccacg ataggtgcag caactgctcc agacaaacca aaaatcgcta atatccctgc
    17521 ttcgaatgtt ccccaattgt aggcgggact gctgatataa aaaatcaacg tagaccaaaa
    17581 gatcgaaaat gttccaaaca tgaaaaatcc actgactgcc gcttctctta atagacgttg
    17641 agaagcaact aaatgcggca aacttttaag tgaatcaagg tatgaaacag ctgtactact
    17701 ttgctggaca gaacgcggca ttttccagta aagcagaagt gtaagaattc cgactgcaca
    17761 taaagcaaat aaatagatca tccgccaaga aaaaagactg gcaacaatac cagataccgt
    17821 ccgagaaagc aaaatcccca cgagtaaacc gctaagcaat gtacccatga ccttccctct
    17881 tgcttttggt cccgctagta ccgcaccgta aggaatgatg atttgcggca cgatcgaaag
    17941 taatcctatt aaaaaagaag cgcatgcaaa taacgtaaac gaaggcgcaa aaaaagcggc
    18001 caacagagag acggcagata acgctgccat tcgaatgatc aattttggac gatccaccac
    18061 gtcccctaaa ggaactaaaa acagtaaccc taacgcatag cctaattggg tcaacatcgt
    18121 caatatcccg actgcacttg tgctaacaga taaactagct gctacttttg ttccaatcgg
    18181 ctggatgtaa tacatattgg caacaacaac gccacaagtg attgataata gtaaaataag
    18241 cgaagttgtt aaaggttttt tttcattact cttcattgat ttcccccatt tcctatctta
    18301 gtttacaact tatggaaaag attggaaata gaaaaataca actattcgcc acacttatta
    18361 tttgtgcttt tgttttcctt gttttttctt gcgatcttta taaatgaaga caactaaccc
    18421 tactagcaca gtaacacttg caaccaacgt gactgcagtt ttgttatatc cgtgaaacag
    18481 agcttctcca ccacacgcat acaacacaga cgaagggatg actccaatca cgatcgacaa
    18541 tatgatttgc tgtagaggca atttcatcgc atctgcagca aaattgacca tagaagatgg
    18601 aataaccggt atcatatagc ctaacattac acctattttt ggatgtttca tttgtcggat
    18661 tgcttgcacc caatgattcg tttcggtttt cttatctaaa aattttaagc gatgcataag
    18721 gaaaattgca gtgatgttac caagagcatt acccaaaatg ttgatgatcg cacctgtcag
    18781 tggtccaaag ctcaaaccag caatgactcc aactactgaa gtaggaatac ctggaacagc
    18841 acacatcacc gctgtcaata cgatcaagag tatcgatgca aaagtcccat gcgaacgaat
    18901 atgtgcgagt agctctgcct tactcgctct cggatttaaa aataattgga tgtctgtctg
    18961 atattctaaa tacaaccgat agcccactac cagtatcaga atgattccca acgagatcaa
    19021 tatcaatcgg attattttct ttttcatatt tcctcctgaa aaaaatctat attcattaaa
    19081 taatagttta acataaaaac aaaaaagcac cgaaatccaa cataggatct cagtgctttt
    19141 aaatagtaaa aattatttaa cagttacaga tgcgccaact tcttcaagtt tagctttcaa
    19201 ttcttcagct tcttctttag atacgccttc tttaactggt gcaggagcgc catcaactac
    19261 agctttagct tctttcaagc ctaaaccagt tgcttcacga actgctttga taactttaac
    19321 tttttggtct ccagctgcag ttaattctac agtgaattct gtttgttctt cagcagcagc
    19381 agcgccacca gcagcagcta cagctacagg agcagcagca gatacgccaa attcttcttc
    19441 aatagcttta actaggtcgt taagttctag gatagttgct tcttttaact cagcaacgat
    19501 gttttcaatg ttcaatgcca ttataatttc ctccgtttaa ttgttctttt ttatttaatg
    19561 ctttatgcgg caattaagct gcatctccat cttctttgtc tgccactgct ttgacagcgt
    19621 aagccacgtt gcggactggc gcttgtagta cagatagcaa catagataga agtccttcgc
    19681 gatttggcaa ttttgccaat gctgtgattt cttccaatga agagacttta ccttcgatga
    19741 taccgccttt gatttctaat gcttttgctt ctttagcaaa ttcgtccatg attttcgctg
    19801 gtgcgactac atcttcgtta ctgaatgcaa cagctgtagg tccagtgaat acttcgtcca
    19861 tgccttcaag gccagctttt ttagctgcac gtgaaagaat agaatttttg ataactttca
    19921 tttctacgtt agcatcacga agttgtttac gtaaacgtgt tacttcatca actgttaatc
    19981 cgcggtagtc aacgactact actgatgcag ctgctgtaaa tttttcgctt acttcatcaa
    20041 cgattgctgc tttttttgcg ataattgctt cactcatttt tgagtttcac ctcctgaatt
    20101 ttgatggaag agttttttgc actgaaaagg caagtcactt ttatagttac tttgctcaac
    20161 cagtattatt catcacctat tcacttcgtt catagtgatt ctactcgctt tgctcgaaag
    20221 gaaactcctt tcgctttgct caaccagtat tgttcatcat ctcttcactt cgttcatagt
    20281 gattcacaat aaaaaactct atgccaccgc agacatagag ggactattga atatattcaa
    20341 taaacgtcct cggtaggaaa ttaaggcttg atgccaccta ctgtcttcgg tacaagttaa
    20401 ttattaacct caactagctt agcatgctaa actagttgag tcaagaatta tttttcagtt
    20461 ggatcaatca acattctaat atttaattag aaagaagctt ggtcaacatg gataccaggt
    20521 ccaaatgttg ttgtgatcgt tacatttttc atgtattgac ctttagctgc tgatggttta
    20581 gcttttagca atacatcatg gattgctgca aagttttcaa ctaatttttc atcgtcaaat
    20641 gaaacttttc cgattggtac atggatattt ccagctttgt ccacacggta agttacttta
    20701 ccagctttca cttcgtttac tgcttttgtt acgtccattg taactgtacc agttttaggg
    20761 tttggcatta agccttttgg tcctaatact cgtcctagac gacctacagt agccatcata
    20821 tcaggtgttg caacaactac atcaaagtca aaccatccgt tttggatttt ttgaaccatg
    20881 tcgtcgtcac ctacgaaatc tgcaccagct gcttcagctt cttttgcttt gtctccttta
    20941 gcaaaaacta aaacagtttg tgttttacca gttccgtttg gtaatacaac agcaccacgg
    21001 atttgttggt ctgctttttt agggtctacg ttcaaacggt atgcaacttc aactgttgca
    21061 tcaaattttg caatgtttgt ttcttttgct aaagctactg cttcggcaac aggataaact
    21121 ttacttgaat caactttttt taatgcatct tgcatttttt tgctcttttt agccattttg
    21181 gttcctcctt gattgtggtt ataacggttg tacctcccac gtgttctata tctttcaata
    21241 tagagccaac tgagaagctg ctgtcttagg gtgtatagat tattctacag tgatccccat
    21301 gcttcgtgca gttccttcaa ccatgcgcat tgctgcttca acgttagctg cgtttaggtc
    21361 ttccattttt aattcagcga tttctcttac ttgatcgcta gaaactttcg caactttatt
    21421 tttgtttggt tcgcctgaac ctttttcgat tttagctgct tttttcaata gaacagcagc
    21481 tggcggtgtt tttgtaacga atgtgaatga acggtcttcg tatacagaaa taacaaccgg
    21541 aatgatcaaa cctgcttgat cagctgtacg agcgttgaat tcttttgtga atcccatgat
    21601 attgataccc gcttgaccta gtgcaggacc tactggtgga gctggagttg ctttacctgc
    21661 aggaatttgc aatttaacga ttttttctac tttctttgcc acgagacata cctccttaag
    21721 tccgtgatgt ggttaattgg agatgcatat ttctcctccc acgttttatc tgatctgaaa
    21781 aaatccagac aaataatgtg caaaacaatg cacactggta cagtatagca aatttatttg
    21841 ttttttcaag atttttttct gctattttta cattttgctt gaataaacgc taaatttatt
    21901 ctaaaaaaac aacaattttt cgttctcatt tcttctttta tgttacactt gcgctagcat
    21961 ggttcaacag atgaaagcaa acaataacac ggttttttag acccttgtac aaagattcct
    22021 acataacaaa atgaggtgga gaaaatgaaa gatttaaggt atagaagtgt ctttgatatt
    22081 attggacctg tgatgattgg tccaagtagc tctcacactg ccggagctgc tagaatcggg
    22141 aaaatcatac gtagtatatt tggcgaacag cctgattctg tagatattta tctatatgaa
    22201 tcatttgcaa aaacctatcg ggggcacgga acagatattg cattagttgg cggcctgtta
    22261 gggatggaac ccgatgatga acgtttagct gattctttga aattggcata tgaagccggt
    22321 atggaagtct tgtttgttcc taagagtgaa aaagccgaac atccaaactc tgtaaagatg
    22381 cttctaaaaa aaggaacacg aaagctttca gtcactggca tttcgatcgg cggtggcaat
    22441 atccagattt ctgaattgaa tggattcaaa atttctttaa gcatgggtac tcctaccttg
    22501 atcgttgtcc accaagatgt tccaggtatg attgctaaag tgactagtat actttctgag
    22561 accaatacga atatcggcac tatgacagtg acacgggaat caaaaggtga aaatgccatt
    22621 atgataattg aagtagatga tcctcaagta gaagaaacgg taaaaagact caaacagctg
    22681 ccaaatatcg acagcgtaaa ttatttcgat taaggagcta aaaaaatgtt tttatcgatt
    22741 gaagaattag tagatcaagc aaaagcttat ccaagtgtag cagaattgat gattgcagtt
    22801 gaaatggaaa tgagtggccg ttcccgtagt cacatcatcg agatcatgga aaaaaacctc
    22861 tcagtaatgg aacaatcaat caacgaggga aaagaaggtg ttacctccgt cacaggaatt
    22921 accggtggtg atgcaaaaaa attagatgat tatttaaatg aggcagactt tttaagtgga
    22981 gaaacaatct taaaagctgt acgcaatgct gtagctgtta atgaagtcaa tgcaaaaatg
    23041 gggttgattt gtgctacacc gactgccgga agcgcgggtg ttgttcctgg tgtgctgatg
    23101 gctgtccgag atcggcttga tttgacacat gaacagcaat tggaattctt atttaccgct
    23161 ggcgctttcg ggttagtcat cgcaaacaac gcttctatca gtggtgcaga agggggatgt
    23221 caagcagaag ttggttctgc aagtgcaatg gcagcagcag ctttggtttg tgcaaaaaat
    23281 gggactcctc atcaagcagc acaagcagta gccattacat tgaaaaatat gatgggactg
    23341 atttgcgatc ccgttgcagg cttagttgaa gtcccgtgtg tcaaacgtaa tgctttaggc
    23401 tcttctcaag cttttatctc tgctgatatg gcattagcgg gtatcgaaag tgtcattccg
    23461 ccggatgaag tcgttgctgc aatgtaccaa gtcggacgtc aaatgccgtc gattttcaaa
    23521 gaaacagcag aaggtggctt agctgttacg ccaactgcta aacgacttgc ggaagagatt
    23581 ttaaataaga gggcgtgata aagctcctta atttcgagta ttaagagatt agttatcttc
    23641 ataacaaaaa acgaagagag gactaattgc ccttctcttc gttttttatt aatccatcaa
    23701 tttcgtattt tttacttcat cgatcgtttt tgttcctgca agttgcatcg tgatcgacaa
    23761 ttctttgttc aggtgttcaa aaacagatgt tactccttct gccccaccta gattcaaccc
    23821 ataaatcact ggtcggccaa tcgcaactaa gtcagcacca cttgccagag ctttgaagac
    23881 atgctctcct cgacggacac cagagtcgaa aatcactggt acacgtttgt tgactacttc
    23941 ggcaattttt ggcaatactt caaaggacgc aggacctcca tctagttgac gtccaccatg
    24001 attggatacc cagataccat ctgcccctgc agagatagcg attactgcat cttctggcga
    24061 ttgtattcct tttacgataa ctggaagatg ggtgatttct ttgattgttt tgatgtcatc
    24121 tggtgtcaag ccttgttttg cagctgcata aatttctgcg attccttttc cttcgccatt
    24181 accacttgca ctttgttcgc tatacgcagc taagttaggc attggtaatg gaaattggaa
    24241 ttgattgatg acgtcttctt cacggtaacc accgagcgtt gaatctgccg ttaaaatgat
    24301 agctttagcc cctgcttcta ctgctttgtt caaaatgaac tcattgaatc tgtcgtcttt
    24361 actcatatat aattggaaaa actgcggtgc gcctggcgct gcatcggctg cgtctttgat
    24421 tgtcgtattt gcataagtgc tgatggaaaa gattgatcca gcagcagcta ctcctttagc
    24481 tgtatcagct tccccttttt catgtgccaa tccttgggcc gctgatggcg cttggataat
    24541 cggtgtatct aattcaattc cgaaaacaga tgtatgcaaa tcggctgagt cgattccgcg
    24601 taatatccgt ggcatgattt tctttgtatt aaaagaagtc gtattttctt tcatcgtcca
    24661 ctcatcttca gaaccaccgc gaatatagcc aaaagctcct gcttccatgc gctcttttac
    24721 acgaccttct agttaggcaa tgtttacaat atcgattgga tgttcttcat tacttgtatg
    24781 ataagtcata gtagatcctt ctttcatctt cttgattaca tataaatcat actaaactta
    24841 caaaaagtaa gcaaacattt actctcagat acttttatcg ctattctttc caaataaatc
    24901 gacttacaag ctgatctact tcttggtttt catgaagcat actatgagag gcattttcac
    24961 ctgtcactac tttttcctct atttcatttc cgcgttgctt caacaacgca tagaccgcta
    25021 atgcactgtt taacggtact gttccatcac tcaagtctgt ttcatctagt tgaccagcaa
    25081 tcaagaaaaa tcgagtggtt ataggaatat taccgatcat ttgctggtaa tcttgatagc
    25141 ggttagaaac gacagcaggc ccctttgcta actcatctgt cagagactgc gcactatcat
    25201 cgacaaaatc attaaaaggc gcgccaattg ctgttaatgt ctgaacttgt ggcaatgacg
    25261 tatcctgtcc atacgttcct aaatagcgta gtgcactaac tcctcccatg gaatgcccca
    25321 ctatgttgac tttatctatg ccgtagtttt ctttcagata caaaagacaa gttttgatcc
    25381 attctgcttg attccactcg ttatttttat tatcttcaaa caatacttgg accatcggat
    25441 tgtctgcttt tccagacaaa tctcctgttg cttgtatttg tccatctgtt cctactgtta
    25501 aaaccaattc ttttactgct tgctgatttt tttccaaacg ccgcaacatc cctttaaagg
    25561 aattttctgt cccgctgtag ccgtggaaaa aaagtgttgg aatagtggtt ttttctatca
    25621 ctttactgga ttctgccgtt ttttctttat cagaaacttc agtagtattt atcttcgtcg
    25681 tctggcaacc acttaacgca aacaaactga taaggactaa agctgctgca gttgcacgtt
    25741 tgcataaatt tttgattggt tctgtcatac acctcttcct ttccatatag aaaaaagaaa
    25801 gacttgaaag gccaatcata tgatcgacat ttcaagtctc tttattaatc gattttatct
    25861 acttgatcga aatcaagttc tgtactggtt tcacgtccaa acatatcgat gttgactttc
    25921 aatttttctt tttctccgtc aacttctgtt acttgtcctt ctaatccagc aaatgcgcct
    25981 tcgatgattt tcacgacttc gccaacttct acttctagat cattttgtcg caggctcata
    26041 ccgatagaac gcaagatatg attgacttct tctggaagta atggtgctgg tttgcttccg
    26101 gctccgtgtg aaccaacgaa tcctgtcaca ccaggtgtgt tgcggacaac ataccatgaa
    26161 gcgtctgtca tgatcatttc aacaagtacg tatcctggga atgttttatg aacgatttct
    26221 ttttctttcc catttttgac ttcagtttct ttttcttcag gaacaacgac acggaaaata
    26281 aagtcggcca ttttcatact ttgcgcacga gattcgatat ttgcttttac tttgttttca
    26341 tatcctgaat atgtatgcaa gacataccag tttttttcaa aagattccat tgaaggctcc
    26401 tcatttcatt gtaaaataaa aaaacctcag tacacccgaa gtttttcttt cgcttcatta
    26461 tagcactaaa agtacgttgt ggctagcgag ttattttaaa atccagccaa ataagctttg
    26521 gatggctgta tccattacgt aaaacatcaa tccgaaaata atagaagttt cgatgactac
    26581 taacgtatct ttacggagtt gttttctgct tggccaagtt acttgtttga tttcatcttt
    26641 tacgcttttt agaaatttca tgagacaacc tcccttgcta ctgtttattt cgtttcttta
    26701 tgtaatgtat attgattaca atatttacaa aacttattga tttccaaacg ttcgccgctt
    26761 tttccttcac tgactgattt tgtgtagttg cgagacccac aaacggaaca agcgagtgct
    26821 gcttttttct ttgccattta cttctgcccc cttgctgaga atcagactta tacttcataa
    26881 cagtatcacg taacttttta cttgtcaatt tgtgaaataa actatctatt gttttggata
    26941 aaattatgat atgattaatt tgaaattaag ctaaaatgag acaattggag gtatagcgat
    27001 gctattaaaa acacttgtat ataagaaaaa agatctgaca accgttacag agactagtac
    27061 attagaagaa gcattaaaaa ttttagaaac ttccggatac cgttgtgtgc cgatcttaga
    27121 ttcttcaggt aatattttcc gtggaaacat ttacaaaatg catatctatc gtcataaagc
    27181 aaatggcggt gatatgacgc ttcctgtcac tcatcttttg aaaaacgcta caaagttcat
    27241 ttacctggat acttctttct tcaaagtatt ctttacgatc aaagagcttc cttatattgc
    27301 tgtccttgac agtgaaaatc atttttatgg tattttgaca catagtacgt tgcttaatat
    27361 gctctctcaa tcttggagcg tagaacaagg aagctatgta ctgacaattg cttccacagg
    27421 aaagcaagga gatttggctg ctattgcaaa aatcatttca aaacacagca gcattgccag
    27481 ctgtattact cttgatatcg aaaaagacga atacattcga cgtacattga tcacgctacc
    27541 ggctaacact gaagcgaaag tatgtgaaac gatcgtcaac cacttagaga aaaagaatta
    27601 taaagtggta gaaattgaaa atttgcaaaa ccaagaagca taatcaaaaa gcgaaaaaag
    27661 aaaacagcta atctgctttc ttttttcgct ttttataatt agacacgctt ttcaaaatca
    27721 agcgcgcgat ctaaataata catcaatggt gctgaaacag ccatgacgat aaactcactt
    27781 aatgcaagcg tgccataggt tgtccaaaat gcttggccac ctgttggaac taacatataa
    27841 gcaatcagtc ccatgctgat ggtaaagaag atcgtgttca atactaaacg catctttaca
    27901 tttttcactt tgttttgcaa taaagctgtc aggcctaaag aaagcaatgt ttggccgcca
    27961 ccaaaaacca catccatgat accaaaacca aagaaggcat tatagacgat gaccccgcct
    28021 aaaactcccc aaagcaattt acggttaaag acaactaaat gattcaaact ttctgaaata
    28081 cgaaactgga tcggccctga ggatacaggc gcaactaaaa tagttaacgc tagataaaga
    28141 gccatgatga tgccatttac aacgattacg gataaccgtt gtctgctttt cgttttttgc
    28201 attttctctc ctcttttctc ctagtttttt taacgcggga tggttgatga accgcggtta
    28261 tgtgaaacaa acaataatta taacagactt ttcatttatt tcaatccgtc ttatctgctt
    28321 ttaaaaaagc aaaagcttaa acaatgtaaa cgtttgctaa aaatcgtggg atatggtata
    28381 aagtaagtgt aaggggtcat aagaattccc agtaaagcag acgaagtagg atctgcaaga
    28441 acagaattgt tgtgaaactt aaataaataa aaaaggatgt gggaatttga gcaacctctt
    28501 actagagaac ggtctagcaa gcagctgaag atctaagtgg acatgccgaa ccttggaact
    28561 tctccttacg taagtaatgg atcatgcgct tgctaggctt ttttgtgttt ttcggtcttc
    28621 ggaccttttt cattttccta gacttctgct ataataacca agaattaatt ttgtaacaga
    28681 tttttttgaa aaaacaagtt tctttcattc aaaaaataga aaaagcttgc taattattgg
    28741 ttaaccagca aacaggagta ttaaaatatc ttgttttatt gaatagcagg aggaaatcat
    28801 gaaattccat tatcatatat tgatcaatcc atcggcaggc agcggaaatg gctataaagt
    28861 agcagaaaga attttaccgg tattaaaaaa taagcatatt gactatacac ttcattatag
    28921 tgaatacaaa ggacacgatg ctgagattgc tgaaactttg gcaaaagaaa cattgattcc
    28981 ttggatagaa gaagaacaga aaacaatcga tactttcccc cttttgatca tcgtaggagg
    29041 tgacggcacc ttacatcagg tacttgatac tttctatcag atggaagtag aattcccagt
    29101 tgcttatatt ccagcaggtt ctggagatga ttttgctcga ggggcaggcc ttccaaaaaa
    29161 tcccaaaaaa ggacttcagc tgattttagc agctcaatca ccagaaaaag tccacataat
    29221 ggcttatgaa gaaaaaataa gtgaaaaaaa aggaatagct gttaataatt tcggtatagg
    29281 attagatgca gctatcgtcc atgcgacaaa tcattcttcc acaaaaaagc gattgaacaa
    29341 atacaactta ggatcatttt cgtatttatt ctctattctc cgtgctttat tcactcaaaa
    29401 aggatttcca attctggtag atgcaggagg aaaacaatta agtttttcaa acgcattttt
    29461 atgtacagca acgaagcatc ctttttttgg cggaggaatc gcgattgttc caactgctga
    29521 tgcgtctaaa ccagtcattg attttgtcgt ggtcgaacga atcaatttct ttaaaatctt
    29581 gtggctcatt cttttactta tacagaaaaa acaattgaaa tcgaaatatt tccatcacta
    29641 cacaacaaac agattacgta tcgtttcaac aacaccgcaa catggtcaag aagatggaga
    29701 agaaatggaa aaacgacctt ttgatatcac aataaccaca actaaacagt tgttttggtg
    29761 ttaataaaag aggttttgtc agaagctaca tctggaacac cgtttattcg gaaaaaagga
    29821 ctgtgacacg acttttgtca cagtcctttt tttactatgc agttaattct ttgcccaatt
    29881 gggttgccat cgatagtttc tttctttacc caaaataaat tggatcattg aaaacaccgc
    29941 agcaaaagca actaagacac aaccattgaa ccaaaaagat tctgcaaatg tttgattgta
    30001 actgagtgtt tcactgatca agttattatt ttgaacaaac caatatgttg gtgtgaatgc
    30061 cgctattttg ctaaccattt ctggcaagta ttctgctggt acaaacaccc caccgataaa
    30121 gcaactgcct gtgataaaaa tatctttgat cccaccgctg gtttctgagt tttttaccaa
    30181 tgaagtgata caagccgaaa agctaatgat cggcaataaa aacaagaaag tatttaaaat
    30241 atgataccat accgttatat cccacgaggt gtggatcact aacagaacca ttagaagtaa
    30301 gaagccactg aacaacagca gcgagtaact aatcaaacta aaggttactt tgcgattcaa
    30361 gtttcgtttg gtaatgggcg cactctggtt tcgtttactg atctctttgc gattaaatgc
    30421 tagattgatg acgctgaaac cgctaaaaat ggatagaaac aacccataag acaagaaatt
    30481 aaaggttgtg cccgtcaaca gctttttcat ccgctgacta tatcccgcac ttagctcaac
    30541 ttctccagta accgagagtg ccatttgtgt ctgttccata gctgcatcga ttgttttatc
    30601 ttcttcttga aacagaatca gtgtttgcaa aaaggtgttc acttgctgtg taacaactgt
    30661 tttagaaaag gcatctgggc gactctgtat cttgatcgtc ggcatctttc ctacttcgac
    30721 ttgttcggta aagtctgcag gcagatgtaa gataatgttt acacgattaa aataaagagc
    30781 atcatcgatt tctttttgtg aggttccatc caatgtaacg actgtttcct tgtcattcaa
    30841 atatttacga aggccatcga ctacaggcga ctcttcttca ctaatcaagg cgattttcgc
    30901 atcttgcaga gaggtatttt caacatcaat attttgcgca aaaaagaatg tggcaacaac
    30961 agtgataatt acacctatca gtaaattcga tttatttgct ttaagcaatg tccaaatcgt
    31021 tttataaagc gtcataacgc acccctcttt ccatccaagc atttcctaac gcaaaaagga
    31081 aaataaatcc gatcaaccat agaagattgg tataaaaact agccaaatca gtgtagaaat
    31141 aaagtttagg cggattttat aatgaactta gccatttaaa ataaaaaaaa cgccacgtga
    31201 attccatgtt atattgaagt taccacaact ctcaatagac aaggatgaat tcacatgacg
    31261 caaacccatg ataacacagt ttcacgcaag ggaaaacatc tttcctactc agaacgttgc
    31321 caaatcgcta tcctgaaaaa ggaaggctat tccaaccgtc aggttgccaa agtgctggga
    31381 cgtgttccgc aaaccataaa caatgagatt gatcgcggaa ccatcaccca gttaaaacgc
    31441 caaacgcaaa atggaaaagt gtatgacacc tactattctg tttatgacgc tgatgcagga
    31501 caggccttct atgaaaagca acgactgaac tgtggcagac gccccaaatg ggcgaacaca
    31561 aatgccttca ttgattgggc agatgacaaa atgttaaatg aaaaatggtc gccggacgtc
    31621 gtggtcggat ttgccagaaa acacgagctg ttcgattgtg ccatcattcc tggcacaacc
    31681 gctctttatc aatggattga ccgcggaatc atgcgaacaa agaacatgga cttgcttgaa
    31741 aaactgtccc gtaaaatcaa agcggtccgc ccaggacacc gtcccaacaa gcgtgttctc
    31801 gggacgtcaa ttgaagaacg ccctaaagaa gttgaaacac gggaaacgtt cggtcattgg
    31861 gaaatcgaca cggtagtggg aaacaagacc aagtcggatg ccgtgctgct gacgctggct
    31921 gaacggcaga cacgctttga agtcatccta aagttgaacg ggaaggacac cgaatcggtg
    31981 gaccaagcgg ttcaacagct aagagaacgg gctggcgcta acttcgatca attatttaag
    32041 accgttacat ccgacaatgg ctcggagttt tctgggctac acgatgctct gcaagatgta
    32101 gctgacgtct atttcagcca cccctatgct tcttgggagc gtgggacgag cgaaaaccag
    32161 cacaaattaa ttcgccgttt cgtccccaag ggaagcccga ttagtgaggt gagcgagaac
    32221 cagattttgc ggatccagcg ttggatgaac gattacccgc ggaaaatttt gggttatgcg
    32281 acaccacatg atacattcgt ccaggcattc aagcaagaac ggttggtggc ttgacccatt
    32341 attccttgat aacgatcctc actcgggaca tggcttcatg tctggaaagc tggcctgggg
    32401 ccagaatcgt ctgaagacta tcatacctca gttcattatc aaggcctcac tgatttagaa
    32461 taacgaggca gttacggtat caaacaaatg agttacctgt
//
LOCUS       DARBDV010000022        32199 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000022 DARBDV010000000
VERSION     DARBDV010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.022"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(136..516)
                     /gene="yajC"
                     /locus_tag="SCM56_000993"
     CDS             complement(136..516)
                     /gene="yajC"
                     /locus_tag="SCM56_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEG1109809.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(613..1758)
                     /gene="tgt"
                     /locus_tag="SCM56_000994"
     CDS             complement(613..1758)
                     /gene="tgt"
                     /locus_tag="SCM56_000994"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEG1109810.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1976..2674
                     /locus_tag="SCM56_000995"
     CDS             1976..2674
                     /locus_tag="SCM56_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEG1109811.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2842..5481)
                     /locus_tag="SCM56_000996"
     CDS             complement(2842..5481)
                     /locus_tag="SCM56_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEG1109812.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6100..7131)
                     /gene="queA"
                     /locus_tag="SCM56_000997"
     CDS             complement(6100..7131)
                     /gene="queA"
                     /locus_tag="SCM56_000997"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEG1109813.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7324..8499
                     /locus_tag="SCM56_000998"
     CDS             7324..8499
                     /locus_tag="SCM56_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HEG1109814.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8503..9144
                     /locus_tag="SCM56_000999"
     CDS             8503..9144
                     /locus_tag="SCM56_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1109815.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9141..10058
                     /locus_tag="SCM56_001000"
     CDS             9141..10058
                     /locus_tag="SCM56_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG1109816.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10055..10726
                     /locus_tag="SCM56_001001"
     CDS             10055..10726
                     /locus_tag="SCM56_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1109817.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11267..11755)
                     /locus_tag="SCM56_001002"
     CDS             complement(11267..11755)
                     /locus_tag="SCM56_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HEG1109818.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11956..12222)
                     /locus_tag="SCM56_001003"
     CDS             complement(11956..12222)
                     /locus_tag="SCM56_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEG1109819.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12222..12485)
                     /locus_tag="SCM56_001004"
     CDS             complement(12222..12485)
                     /locus_tag="SCM56_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEG1109820.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            12961..13146
                     /locus_tag="SCM56_001005"
     CDS             12961..13146
                     /locus_tag="SCM56_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEG1109821.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(13288..15111)
                     /locus_tag="SCM56_001006"
     CDS             complement(13288..15111)
                     /locus_tag="SCM56_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEG1109822.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(15709..18414)
                     /locus_tag="SCM56_001007"
     CDS             complement(15709..18414)
                     /locus_tag="SCM56_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEG1109823.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(18720..19667)
                     /locus_tag="SCM56_001008"
     CDS             complement(18720..19667)
                     /locus_tag="SCM56_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEG1109824.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            19857..20126
                     /gene="rpsN"
                     /locus_tag="SCM56_001009"
     CDS             19857..20126
                     /gene="rpsN"
                     /locus_tag="SCM56_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEG1109825.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(20302..20664)
                     /locus_tag="SCM56_001010"
     CDS             complement(20302..20664)
                     /locus_tag="SCM56_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEG1109826.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(20675..21796)
                     /locus_tag="SCM56_001011"
     CDS             complement(20675..21796)
                     /locus_tag="SCM56_001011"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEG1109827.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(21812..22162)
                     /locus_tag="SCM56_001012"
     CDS             complement(21812..22162)
                     /locus_tag="SCM56_001012"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEG1109828.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(22292..23803)
                     /locus_tag="SCM56_001013"
     CDS             complement(22292..23803)
                     /locus_tag="SCM56_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEG1109829.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(24125..25477)
                     /locus_tag="SCM56_001014"
     CDS             complement(24125..25477)
                     /locus_tag="SCM56_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEG1109830.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(25630..26706)
                     /locus_tag="SCM56_001015"
     CDS             complement(25630..26706)
                     /locus_tag="SCM56_001015"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEG1109831.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            26902..28647
                     /locus_tag="SCM56_001016"
     CDS             26902..28647
                     /locus_tag="SCM56_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEG1109832.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(28927..29364)
                     /locus_tag="SCM56_001017"
     CDS             complement(28927..29364)
                     /locus_tag="SCM56_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HEG1109833.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(29348..30721)
                     /locus_tag="SCM56_001018"
     CDS             complement(29348..30721)
                     /locus_tag="SCM56_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109834.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(31043..31531)
                     /locus_tag="SCM56_001019"
     CDS             complement(31043..31531)
                     /locus_tag="SCM56_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1109835.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(31553..32155)
                     /locus_tag="SCM56_001020"
     CDS             complement(31553..32155)
                     /locus_tag="SCM56_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEG1109836.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         9205 a         6883 c         5942 g        10169 t
ORIGIN      
        1 attattattt atatgacaca cccgattatt caatattgag caatatatcc catgctctta
       61 tctctttctt ttttgaataa aaaatgaggt aggacatctt tctgccctac ctcgtcttta
      121 aataagcttt gatacttatt tttcttcctt ctcttctgtc tcttccagcg tttctgttgt
      181 ttcagttttt acttcaactg tctcagctgg ttcttctgtt gtttcaacag tagtcgttgc
      241 atcatttgtt acgacagtcc ctggtttgac cgttttgatt gctgcacgat cgtactcaag
      301 gaagatccct tcgcagtcta aaacaactgt ccgtttgtca ttgtcgattt ctgaaacaac
      361 tccatgtaat ccgccgatcg ttacgactga atcacctggt ttcatgctat ctaacaagtt
      421 ttgacgttct tgctgttgtt tcttttgcga acgtgacata aaaaaccaca ttgctaatag
      481 cgcgataaac atcacaagca ttggtaatgt tcccattttt ttcacctcac taattctgta
      541 tctacaaaaa acactttagc agaaaaatgc tgataacact agttttttac ttaattttta
      601 aaagattttc ctttagaagc tttttgcgtt ttctttattg aacccgtatt cttcaaaaaa
      661 ggcttctcta aattcaagca aattatcatc catgattgct tgacgtactt gtttcattag
      721 attcaacaag aagtataaat tgtggtaaga agtcaaacga atcccaaatg tttcatcaca
      781 tttgatcaaa tggcggatat aggcacgtgt atagttttta catgtataac aatcacattt
      841 ctcatcaatt ggacggaaat catgggcata tttcgcattt ttcactacta gacggccttg
      901 agaagtcata catgtgccat ttcgagcgat tcgagtaggc aagacacagt caaacatgtc
      961 tactccacgg ataacaccat cgatcaatga atcagcagca ccgacaccca tcaagtaacg
     1021 tggtttgttt tcggggatca acggtgtagt gaattctaat acacgattca tttctgattt
     1081 aggttccccg acagataacc cgccgattga gtaaccaggg aaatccatcc cgactagatc
     1141 tttcgcgctt tgacgacgaa gatcttcaaa ccctgctcct tggatgattc caaataatcc
     1201 ttgacggtct ggattagcat gagctttcaa tcctcgttct gcccatcgag acgtacgttc
     1261 tactgatttt ttcacgtagt cgtaactttc atcgaatggt ggacattcgt caaaactcat
     1321 catgatatca gagcccaatt tattttggat gttgattgct ttttctggtg ataaaaacat
     1381 acgcgaaccg ttcaagtgat ttttgaaatg cacgccttct tcttcgatat tccgcatatc
     1441 acttaatgaa aaaacttgaa agccgcctga atctgttaaa atcggttgat cccagttcat
     1501 aaatttatgc agtccgccag cttcttctac taagtcttca ccagggcgta gccataaatg
     1561 atatgtatta cttaaaataa cgccagcacc catttctttc aactcttctg gggacattgt
     1621 ttttactgta gctaatgttc ctactggcat gaacattggt gtggggaaag ttccgtgtgg
     1681 cgtgatcaat tcccctaaac gcgcgccagt atgtttttct ttcttgatta aacgatagcg
     1741 aatggcaggt tgcgtcattc atttccctac tttctatata aaatatcttg acaactccca
     1801 ccatcataat cgtttcaagt gctttttgca agttccctcg gcttccttct actttattta
     1861 atattctttt ttttatcctt tttttatgaa taacaaaaaa aagaaaaaca ttatgacaga
     1921 aatatttttt gatacatggt atattggtgt tattaaagaa aaggagggaa aaatcatgca
     1981 atcttctgca gaattaaaac gccaagcaaa agagagtcta aaaggacgtt ggggacaagc
     2041 tgttttacta aatctaatcc ctacattgat tggtattgct actattcttc ttattatgat
     2101 tcctattatc ttgtttgcca ccaccatata tgatggatca gcgagcgtct cagaagcgac
     2161 aacttcagga ggcggtagtt caggaggcgg tggcggaatt atttccagca tcattagcgc
     2221 tttgttcatg agcggtattt cttggactta tcttgatatt ttacgtggac aaaaagatca
     2281 gatcgaacca ttcaaagatg catttcgtgg attcaaaggt ttgtttatcg gcggagtgat
     2341 cagcttagct gtgctgatct cgatctttac aacattatgg ggtttgctat tagttattcc
     2401 tggaatcatt aaaagctatt cttattctca aagctatttt atttactacg atatcgtcca
     2461 agaaacagga caaacaccta aagtactgga tacgatcact gcaagtcgaa aattgatgga
     2521 tggttataaa ggacaacttt tctggttaga tctttcattt atcggctggc atatcttagc
     2581 attagcaact gctggtattg gttatctatg gctcagccca tacatttctg caacaaaagc
     2641 agcattttat gaacaattgc caaaagatat ttaaaagaga gtgtgacttg tgaccgaggg
     2701 ggtggcagga gaacaccgtt tatcgagagg cagtaaaaga aacattcagc ccattagcaa
     2761 aaaggaggtg tgacaacatt catgtcacac ctcctttttg tatagttcca gtaaaaagaa
     2821 aatcaactta gaaacagtta atcattcctc ttcttctgct tccggtaatc gatggaactt
     2881 gatcagttca ttcaccgcga ttggtacgat agataaaatc cctaccaaga accagtgatt
     2941 caaactgatg tgtactaggc cgaatagttc ttgcgtaaat ggcagcaaag caacagccgt
     3001 tgtaatagct aatgccagta acgccatttc taccaaagct ttattggtcg ctaaattgac
     3061 tttgaagatc gattggctgc tgcgtacatt gaacacatgc agaatcgatg aataagctaa
     3121 gatcaagaat gcaacagttt gaccgacatg ctgacttgcg tctacccatg ggctgatagt
     3181 atcgacataa ctacctaaat aataaccaaa caaggttacg atggtgaaga cccctgcatt
     3241 tatggcaatt ttttgcaata aacctcgagc aaagatccct tcatttttcg gaatgggtgg
     3301 ctgatccatg atgccgtgtt ccgctggctc tcttcctaaa gcaaatcctg gaagtccatc
     3361 agcaactaca ttgacaaaca gtagttgcac agctgtaaat ggtgctcccc atcctaacag
     3421 catcgcaatc aaaacaataa agatctccga aatattacag cttagcaaaa agttgatagc
     3481 tttacgaata ttctgataaa ccgaacgtcc ttgactgaca gcgtctacga tcgtcgcaaa
     3541 gttgtcatcc gtcaaaatca tatctgcggc actttgcgcc acatctgttc ctgtgatccc
     3601 catggcacac ccaacgttac ttgctttcaa cgctggtgca tcattcacac catccccagt
     3661 catcgctacg actgcacccg tacgttgcca agatttgacg atccggatct tgtcttctgg
     3721 cgttacccga gcatagacag agagagcttt gacacgcgca tctaattctt cgtctgataa
     3781 ttgatgcagt tctgatcctg aaagtgcttc tgacggatca ttcaagatac ctaattcttt
     3841 cgctatggca ctggcagtca ccacatgatc tccagtgatc atgactgttt tgatgccagc
     3901 ctttttcgct cgcttgattg ctcctttact ttctggacgt ggtggatcga tcattccgat
     3961 cagaccaatc aaacgtaagt ctttttccag tgcttcagaa gaaatatctt gcggttcttc
     4021 gtcataagta gcataaccaa cagcaatcac tctcagtgcc cgtttaccaa agcgatcatt
     4081 aacgatcgcc gcttgttcta catcgcctga actaaagcgc ggagcaagaa catcgaatgc
     4141 tcctttggtg attgagatgt atttttcgcc catttggtga actgttgtca tcatttttcg
     4201 ttcagaatca aaaggaagct cgtttactcg tggatatttt tcttctaatt ctgcctttgt
     4261 atgataattc tcttcaacag cccttacgat ggctacttct gttggattac ctttgataac
     4321 aaggtcgtca tcttcttgct cgaccgtcac atctgtacat agtgcagcca ttttcaatat
     4381 ttccatcgcg ctgttcgtca ttgaatcttc gatattcgtt acttcatctt ctttgctcca
     4441 tacccggcgg acgcgcattt cattttgtgt cagcgttcct gttttgtctg agcagataac
     4501 attagcggtt cctagcgttt caactgctgg caactgtcgg atgatcgcat gtttcctcgc
     4561 catcttctgc acaccgtatg caagtgtcaa cgtaacaata acagtcaagg tttccggtac
     4621 agctgcaaca gccaaggaaa tcgaagtcat gaacatctca aggagtggtt ctccttgcag
     4681 ttcaccaatc acaaatacta aagccgcagc cgctaaagcg atcatactga ttcgtttgcc
     4741 tagctgattc aatcggacct gtaaaggtgt tttttgcata gtattttcgt tcaaaagatc
     4801 cgcgatctta cccatttctg tagccatgcc aatggcagtg acgatcgctt tcccacgacc
     4861 tgcagcaacc gtacatcctt taaataccat attaagttgg tcacctaatg actcatcttc
     4921 ttcagcaata aatgctgcat ctttttcaac tgcttcactt tctccagtca acgccgattc
     4981 ttctacacgt aattgtgatg cttcgatcaa acgggcatcg gcagaaatag catcaccgga
     5041 ctctaaaaca agaatatcac ccactaccag ttgctctgac tcaacagtgg tcactttgcc
     5101 gtctcgtatg acagttgccg tttgtttatt catatcttgc aaggattcca gtgctttttc
     5161 tgcgtttcct tcttggacaa tcgccaatac cgagttgatc acaacgatag cgatgatcag
     5221 cagtccttca aataaatccc catgttctgt agcaaatgcg gtataaaagg aaatcaccgc
     5281 tgctacaaga aggatgatcg ttgtaaaatc ggacaagctt cgaaagaatt ttttgatcat
     5341 cgattctttc ggcgcctcat cgaatttatt caatccctgc ttttttcgcc gttcatccac
     5401 ttcttgtgaa gacaacccag tatctggatc tgcttctgtt accttcttta cttcttctat
     5461 tgtctgcttg tattcttcca tcctattcct cctttaaaat cttcgtacaa aaaaaggtac
     5521 ttattttact ataacatcct ttttttattt acttcaaata aagggcttat cgattatttg
     5581 ttaagaaagc gtaaaattta aagtgcttat atttgcaaac gctaccaaag gatgctatta
     5641 tatagatgta aggaaggagg ataccatcaa atagacagaa gaatttctcg atagaacagt
     5701 gtaagcaaac aaagttgtta gaagtacagg tttcacccag acatgcgtgt agagtacaaa
     5761 cctataaata gtgaatgcgt atggatgttt ggatcacgaa gtatccgaaa caagaagtaa
     5821 tgcgatacgt aacaattgaa taacgtaaac gtctgaaaat tgaataggaa caagagatgt
     5881 ttcaaatgtt gaagatgtaa cgaaaacaaa atttttgttt aacatgaaga aattcaacac
     5941 cgtgctttgc atgtatgatg gaaagttaca atcatttctg tttatttgaa tccttccgag
     6001 gtacaagaaa gccgagctta tcgagagctc ggctttttct ttgtcataaa atctttgtaa
     6061 agcttttttg ttattcaaaa ctagcggctt ttcataacct tatttcacga acatcgcatc
     6121 tccaaaacta aagaaacgat agcgctcttc aatcgcatgc tggtaagcag ataaagtcaa
     6181 ttctcttcct gcaaaggcac taactaacat gactaaagtc gattttggca aatggaaatt
     6241 agttgagaaa gcttctacca ctttgaattc atagccaggc gtaatgaaaa tatcagtcca
     6301 tccgctgtct gctttgattt ctccattaaa tttcgttcca atcgtttcta atgttcgaat
     6361 tgaagttgtt ccgacagcca cgattttccc gccattttta cgtacttggt tcaatcgctc
     6421 agccgcttct tccgtcaaac ggtagaattc actatgcata tggtgttctt caattttatc
     6481 tacgctgaca ggtcggaatg tacctaaccc tacatgtagt gtcagataga ctaattcaac
     6541 accctttgct tcgatctcat caagtaattc tttcgtaaaa tgcaatccag ctgttggcgc
     6601 tgcagcagaa ccattttctt tcgcatagac agtttgatag cgatctgggt cttccagacg
     6661 ttctttgata tatggaggca atggcatttc tcccaatgat tcaagaatct ctagaaaaat
     6721 tccttcatac tcaaagtcaa tgatccgtcc gccgtgttcc aattcttctt tcactactgc
     6781 ttgcaatctg ccatcaccaa aagagattct tgtcccaact ttggcacgtt tcgcaggttt
     6841 gatcaatgtc tcccacgtat ctccttgtgt attcgttaga agaaggactt ctaaatgcgc
     6901 accagtatct ggtttttctc catataaacg agcaggcaga actcgtgtat tattcataac
     6961 tagcgcatca ccaggctgta actcttcaat gatttcatgg aaatgtttat cttccatctc
     7021 tccagttttc cgatccaaaa caagtaaacg agaatgatcg cggtctttta atggtgtttg
     7081 tgcgattaat tcttccggca agtcaaaatc aaaatcttct gttgttaaca tatcgtcact
     7141 cactttctat catgtgcttc atcattctat catgtttttc gttctgttgg caaaatcagc
     7201 actttttcac ttctattttt tgttttccta aataaaaaaa ctaccctatt tgtatttttt
     7261 cattgtttta ggcatactag taatagatag ttcttcattg aaaatagaaa aagaggtggc
     7321 accatgattg aatttcaaaa tgtgtcaaaa atttataaag gtggaaaagt cgctgtagag
     7381 gacgtcaatc tttcatttga aaagggtgag ttcatttgtt ttatcggtac gagcggaagc
     7441 ggaaaaacga cgacgatgcg gatgatcaac cgaatgactg atccaacaca agggaaaatt
     7501 ttgattgatg gaaaatccat ccaagaaatt gatccagtgg aattgcgacg tcagatcggc
     7561 tatgttatcc aaagtattgg attgatgcct cacatgacga tccgtgaaaa tatcactctt
     7621 gtccaaaaat tgttgaaagt cagtcaagaa gaacggaata aaacagctga aaaaatgatt
     7681 gatcttgtgg aattgccccg tgagatgtta gatcgctacc cacatgaatt atctggtggt
     7741 cagcagcagc gtattggagt cgttcgtgcc cttgcagcga atcaagatat catcttgatg
     7801 gacgagccat tcggtgcgct tgatccaatc actcgtgatt ccttgcaaga ccttgtcaaa
     7861 gatttacaag agcgtttggg aaaaacgatc gtatttgtaa cccatgacat ggatgaagcg
     7921 attaaactag caagccgtat cgccatcatg agtgaaggcc gtgtgatcca atttgataca
     7981 ccagaaaata ttttacgtca tcctgcaaac gatttcgtag aagagttgat tggagaagat
     8041 cgcctactac aagccaagcc aaatgcgaca agagtcggcg aagttatgct aaatacagca
     8101 atcacgatta caccggaaaa atcattacaa gaagcaatca aactgatgag ggaaaaacgt
     8161 gtagatacgt tgttagttac cgataattcg aatgtattaa aaggatttat cgatgtagaa
     8221 acgttgaata aaaaacgagg aaaagtatcc agtgtcggcg acattttgaa taaacatgtt
     8281 ttttacgtaa aagaatccgc ctatcttcgt gataccttgc aacgcatttt aaaacgcggg
     8341 ctgaaatacg tgccggtcgt tgatgaacag aacaaagtcg taggtatcct gactcgtgca
     8401 tcattagtgg atatcgtcta cgatgtgatt tggggagaag atcccgcaac tactgatgtt
     8461 gcagagtcag ctactcctga agaatctaag gaggtctgac ctatgcagca gttttttcat
     8521 cagcacggtt cagaaattct ctcaaaaggt tttgaacatc tttatatttc aggacttgct
     8581 ttgatcattg gtatcatcat tgctgtaccg cttggtgtct tactgacacg ttttccaaaa
     8641 gctgccacga ttgtgattgg cttaaccagt gccttgcaga caatcccttc tcttgcttta
     8701 ttggcattga tgatcccgct atttggcgta ggtaaattac ctgcgattat cgcactcttt
     8761 atttattcat tattgccgat tttacgtaat acgtacattg ggatgaaaaa tgtcgattgg
     8821 aattatcggg atgtggcaaa aggcatgggg atgactgagt tccaatccat tttttccgtt
     8881 gaattgccca ttgctatgcc aacgatcatg gctggtattc gtttagctgc cgtttacgtg
     8941 attgcatggg ctacacttgc ctcttatatc ggcgctggtg gtttaggcga tttgattttt
     9001 agtggactga ataattacca accagatctc attttggcag gtacgatccc tgtaacaatt
     9061 ttagcactat tggctgattg gcttctaggt ctcttggaac accggctgac accgatcgca
     9121 cttagagagg agaatgaaga atgaaacgta tactaaaaat gaccatggtt cttttctctc
     9181 ttcttatact tgcgagttgt tccttccctg gtcttgccag caatacagat gaagacacca
     9241 tttcgatcac aggtggaatc acttcagaag cacagatctt agcaagtcta gttgccggaa
     9301 tggttgaaca ttatacagat aaaaataccg caatcatcaa caatctggct actacaacta
     9361 tcaaccatca agcgatgatg aacggagatg cttcaatttc agcggctcgc tacacaggaa
     9421 cagatttaac gactacattg aaccttcctc ctgaaaaaga tccgaaaaaa gcgtttgcta
     9481 ccgtgaaaga tgaatttgag aaaagatacg gtcaaacgtg gttcccgtcc tatagttttg
     9541 aaaatacgta tgtctttctt gttcgaaaag acaccgcaca aaaataccat ctatctaaag
     9601 tcagcgatct gaaaaatgta gcggatgaat tagtagctgg tgtagatact tcttggatca
     9661 atcgtaaagg agacggttat gacggcttcc aagaaactta tggcttctcc ttcaattcga
     9721 tattacctat gcagattgga cttgtttatg acgcagtcga agcaggaaaa atggatattg
     9781 ttcttggcta ctctacggat ggacggatcg ccagttatga tctagtcatg ttggaagatg
     9841 atcgtcaatt cttcccacca tatgatgctg ctccagttgt agacaacaag atattagctg
     9901 atacgcctca tttaaaagaa gccttaaata agttaggtca taccatcagt acggaaaaaa
     9961 tgcaggaatt gaactacgaa gcagataata atctagtgga accatcagta gtagcacaac
    10021 gtttcttaca agaaaaccac tattttgaag ggaagtgaag caattggata aaatgaatgt
    10081 ctttcaacaa tttttctatt atttccagga aaatggctcc tatatctttg cacaatttat
    10141 ccgtcacttc ctgatttcta tctatggtgt cctctttgca gcagttgtcg ggattccggt
    10201 tgggatcatg atttctagga gacgtaaatt agcaaactgg gtgattcgtt tggcgaatat
    10261 catccagacg atcccttctc tagccatgat ctccatttta atcattggat taggcctggg
    10321 agtcaatgtc gtgattgtta ctgtattttt gtattccctc ttaccgatca tcaagaatac
    10381 gtataccggg atgatccaag tagacaaaaa catccttgat gtcggcaaag gcatggggat
    10441 gactgctaga caacgactat tcatggtaga gttaccactc tctgtttctg ttatcatggc
    10501 aggcataaga aacgcgttag ttgttgccat cgggataacc gcgatcggtg cattcgtcgg
    10561 tgctggtggt cttggagata tcattatccg aggaaccaat gcaacagagg gcacatcgat
    10621 tattttagca ggtgcattgc caactgcatt aatggcgatc atcacggact ggcttttagg
    10681 tattttggaa agacggctag atccagccag ccgtacatca cgctgatgtt attgtaaaaa
    10741 acaaaagctt ctgcgaccat ttgttggtcg caaaggcttt tttagttctg tcatttttaa
    10801 ggcaataatc cactctgatt cttccctcct tatgggtaaa gagttgcttt tcttagctat
    10861 ttatccattc cgtatactcc aacttcatac tgccaacggg ttcatatctg catccaccga
    10921 ctaagttttt ttcttatccg ctaaggcatt ttgccaatcc attttcccat cactcgtcat
    10981 ggaattcata ccacatgatc tctaaatact cgtctgcttg ttctttgaat tgggcatcaa
    11041 ttcttacatc ctttggcgtg gctttcattt aagccttttc catcagtcct cgattgttcg
    11101 atgccttcag ctgcatcatc aaaactggtg actgccgtat cggtaacttc gatcatcttt
    11161 tatacaatcg ggaacttgcc atcctcatcc ttggctaaaa acatatctag ccattccagc
    11221 ttctcgccat gggaggtttg cggttcctaa aaccggtaca cgattcttat ggactgcatc
    11281 cgtcgcaact agacttggcg atacgatgat ccttcaccag aagagcaccc ccgtaaacca
    11341 attgatcgat atattgccaa taagaaaaga cattggcgta aaagatatcg atccctcgtg
    11401 atgcattgcc gttgatactg ctattcatgg ccgaaagcgc cacgacttta acttcttccg
    11461 tttgagtgac attcatgttc ggcaatgtcg ctttggatac ccgctctgct aaaggaacga
    11521 cactggtatt gtatttcgta tctgtatctt ttccaaaaga ccatgccaac gaatcctctg
    11581 ggaaccaata agacgactcc ggttgattgt ccattgcttt tttcaacata cttttggctg
    11641 tttctcgata aacgacattt ccaattgtct tcgcctcact ttggcgataa tcgatggtcg
    11701 ttgccactgc caacactaac aacgatgctc caattttact gattttcctt gtcacacttc
    11761 ttcctccttg ttttacataa acccgtcaac attccattcc taaactcaaa caatgaccct
    11821 tcaaaggtgg agatatagga acaaattaaa gaaatcatgc aataaatcca agaaggttgg
    11881 ttttagaaca aaaaaagagt ctcctttttg gggagactct ttgttaacta tctaaaaagg
    11941 ttggctcttc tcacttcact cataatgtgt ccaggcagaa taaatttcta caactttatt
    12001 ttcctcatca actttgtaaa ccactcgatg ctgtctattc agcctgcgtg agtatctgcc
    12061 ttcatgtgta ggtctcagct tttcaaaaga ctgcgtgggc atatatggat cttccttcaa
    12121 tgtctgaatc acttcttcaa attgtttctt taaattcgtt tgcttgattt ttcttaaatc
    12181 cactttcgca gagtttttga ttgcgactgt atagttactc atcaaagagc ttcccaatca
    12241 atatcatcga tattcgtaaa gctactgtcg tctttttcgc ggtctcgtac tttatctaaa
    12301 gtacctgttt gctcaagaaa aagggtttct tgaatcgctc tccagtcttc cagcccaatc
    12361 aatacagcat tgtttccact acgatcacta ataatctcga tttcagtatg attttcattt
    12421 acctctttca ataactgata aaaattttta cgagcagtgc taggattcat cgttgtatta
    12481 gccattgtct ctcctccttt ttctgatata attatagcgt acttgattaa gtacgtcaaa
    12541 aaaatacaat aaagcactta aattcagcaa acttttcctc ttttttacat agagaaaaag
    12601 accccttttt agggaggatc tttgtccacg ctcacttctt actcctttac gactagcggt
    12661 agccaaacgt aaagtaattg gtaacaacgt atcatcttta gcaaaggtcg aatccaaagc
    12721 ctgtgagtct atgcaacaac accaatacac gaaggttcct cgttgcagtg tgacttgatt
    12781 gatatcttga ataacctgag gatacgcacg aaagatttgt aacggttatt caaaactgat
    12841 tttggccgtc aagtcttcta ctgcctcaaa aatgattgcc tctttcgtct cctgtcattc
    12901 atgttcacta tcgatttgat acttgcttag accgttgggt ttgtgaatca ccaattaaaa
    12961 atggcactga gttatggtaa caaaaaatgg tcacttgacg aggctgagat tcctacttat
    13021 gaagacacat ggcacttaag agtcgaggta aaggaagaag ctgaaaatat aggaggctgt
    13081 acattatttg gcaaaggatt cggaaattat gatcaagaca tcaaaatcaa aatgttttac
    13141 agctaaatag gaagaataat aacatttcat acgctgttcc catctttaat ggcttataga
    13201 gggcaccttt agctgagaat aagacaaaac agaccctcta gcttcaatta agagctagag
    13261 ggtctgtttt tgattttaaa acttggttta tttcttcaaa tggtagctat aggtgctgac
    13321 aacgatattt tcacgccaag acaatcgcaa tcttaaccgc aaacttgttt ggttatggag
    13381 aatattctgc cagcgtctgt ttggtacaaa ttgaggaatc aacacagtag ttgtatagtt
    13441 ttgtttcgct gcatttttac ttacgatatc tacataacgg ataattggat tttcaatcga
    13501 gcgataagaa gagtggacga ttgaaaaacg gacatctggg aaatgctttt taaattctgc
    13561 ttcgatttct ttttcttttt cgacatcttc gtctagcgaa acatgcatcg caacgacata
    13621 atcaccgatc gaacgtgcgt aattcaacgc accgatattt acccgtgtca cattcccgac
    13681 taatacaaga acggtattcc cgtcataatc atgtagtttt gcatcatttt ctaaacgaag
    13741 ctgttctgct acttttttat aatgatcatg gatcttataa aaaatgaaca taacgattgg
    13801 cataatgatg aagaaaggcc aaatgtcccc taaccgatag acaaacaaaa tcgcaatgat
    13861 tgcatatgag atgaacgcac ccgtaatatt ggcaatcgat ttgcttaacc atctttttcc
    13921 ttcttttttc catttgacga ccatccctgt ttgagataaa gcaaatggaa tgaacacgcc
    13981 gatagagtat aacggaatca agcgttctgt tgagccatgg aaaataaaca gcaatacgat
    14041 cgaacctagc gctagtgtga taatcccatt ggaatagcct agtcgatccc cacgatcttg
    14101 atacatgtga ggcatgaatt tatcttttgc cagattgtat gcaagaactg gaaaagcaga
    14161 aaaaccggtg ttagcagcaa ctgctaaaat cagcgctgta gcaaattgaa ggacgtaata
    14221 caaaatacca tgtccaaaga cagctttacc aatttgagac aaaacagtga cttctttttc
    14281 aggaacgatc ccataccaat agttgataaa tgtgatcccc acgaagaaga aacctaagat
    14341 caatgccatc atcgtcaatg tagcagcggc attttttgct cttggcttct taaagaatgg
    14401 tacagcatta ctaatcgcct ccaccccagt taaagaggaa gaaccagaag aaaatgctcg
    14461 taaaatcaaa gcgatagaaa cacccggcac agccgctcct ggaagtgcag tagcttgcaa
    14521 tggcacagca cctgtcacga tattatataa accaacaacg atcagtaacg agatcacaag
    14581 gataaatgtg tacaccggaa ataataggaa cgatgcagat tccctcagcc cacgaagatt
    14641 cagcatcatc agtagcaaaa cgattgtcac agaaatagcg acttgatgac cgtagagtgc
    14701 tggcaccgct gatgtgatcg cttcagcacc tgctgagaca gatactgcta ctgtcaacat
    14761 gtagtcgatc aataaagagc caccggaaat caagccagca ttctttccaa gattctcact
    14821 gctgacgaca tatgctcccc cgccatgagg ataagcatgg atgatttgac gatatgatag
    14881 cgttaatgaa atcaataaaa taataacaaa agcggcaatg ggtagcgaat accagatggc
    14941 tgcagcagac aaagcgacta aaacgacaac gatttgttcc gtaccatatg caatagatga
    15001 taacgcatca gacgacaata atgccaaagc agcaaatcgg gttaatttgt gctcatcatt
    15061 ttcagcagat tttaaaggct tacctactaa caaccttttt aagtaatcca atttctaaac
    15121 tcctttacac acaattttac ttcaagtttt atagtcgaaa atttatagta aacaagatgt
    15181 tatagtacgc tcactgacct taaaagtcaa taaactttaa gaaaataatt ggaaaaaaac
    15241 agaaaaatat caagcgcttt ttcaaacgct ttaatacagc aaaaatctaa cattttttta
    15301 tgggcttttt ctttgaattc tataataaaa ggatagaact tgacttagaa aatcaagttc
    15361 tatccttttt gatatcttaa cttttggaag agagatgtaa ggctgctttc tacccatcat
    15421 tcttactact cgaagcatag cgcagttgtc acaacctctt gataaacggt gtttaaaagc
    15481 ttgcatctgc ggtaataagc ccaaccaaat taaaaacata agaagctgtt tttatttggt
    15541 cgttcttatt acttgatgca gagcagcttt tttacaacct cttggtacac ggtgttcaat
    15601 cagctgaccc tcggtattag ctcaaaaaaa cggcaaaata tgaggaacta tttttccatt
    15661 tttttctcta atactcaggt caaaacactg atttcacaac ctcttttttt aatgaaaaat
    15721 atggctctca tgcgccgctt ctttgaattt cgtcattttt tcttcgatga acggatagat
    15781 tgccgttcct acgattccaa agacaacaaa caaaccgatc atgatccaga ttgctttcgt
    15841 agcatttggc caataaattc ctcctgcaga ttcccgtaac aagtttaccg catgagtaaa
    15901 tggcaagaac ggattgatca tttggaagaa tttccctgag acttggatcg gatagttccc
    15961 gcctccgcca gagatcgaca gtactaagat gataatagcg atccctttac cgacattatc
    16021 gaataatgca gccaagacat agacgatcat catgaacgct aaggcgatca agacagcaaa
    16081 tgacacgctg tagaccggat tttggacatc tactcctagt aagaaaatat tccctaatgt
    16141 aacgatcaag ctttgcgcaa tcgccattgt caaaaatgtc agcatacgag caacgaattg
    16201 ttctcgtttc gtaaaattgt ttcggtcttt ctttcctaaa tagtattctg tcgtagcaac
    16261 acttgaaaga agaacagcgc cgacccacag acatagtgca gtataaaacg gggtgctagc
    16321 tgaaccgtta tttgcgattg gataaagcgc attggtttgt agctcaacag gttgtgtaaa
    16381 gaagtcactt tcttttgttg cgtctagttt caataatttg atgacttcgc ctagatcgac
    16441 ttcttgttcg ccttttcgga tcgcgtctgc tgctttttgg ataccagctt tgatatttgg
    16501 ccagtcattg ttgatcaagt ccgcagcagc gttcacagct gtttcgactt ctggcatttt
    16561 ttcatttact gttgtcaatg taccggtgat ttcatttttt agtgctggcc aatcattttg
    16621 gataaaatca gcggccaatc ctaatttttg ctgaatgatc ggtaattcat tgttgtatag
    16681 gtctgcccct tcattgatgc cattgacgat ggtatccata tttccattca gcattgtatt
    16741 ggcatcatgg atttcttgtc caattgcagg catttctttt tgatattttt ctaaaattgc
    16801 cactgcattc gttacagttg ctttcgttga gctgagaagc gaatcaaagt cgatctggtc
    16861 cgctttgctc agtgcgcctt gtgcagtccc aatcgtatcg atgacttggt ctagtagaga
    16921 agtgacagtg gaactgattt gacctgtatt gataccagca atgatgccat tgatattatt
    16981 aacaacggca tcaatttgat ctagcaattc atcaatagcc ttcgtgtctt cattttgaac
    17041 agctgtgttc aaagcagata ggcggttttg cagattgctt agcaagggtt ttaattgatt
    17101 tagctgatca ataattggct gtaatttcgt atttccagca cttgtttgca atttagtcag
    17161 gaactcgacc agtttatcga ttgcattttg ttgttgagat attccgtcaa taaagccgtt
    17221 caatacattg ctgatgtttt tcttttcatc atcagtcagc ttaccatctt caatagctgt
    17281 tcttacagta tctattaacg aacctgtcgt tgttgccact tgtccaattg acttcaatgt
    17341 aacatctaca ctgtttgtga tgcttggtat cgcatctttc agctgagagg cacctgattt
    17401 tgtttggttt gctagatcac ttgcctgatt ttctaaattt ttcacatctg gcaagatatc
    17461 ttgtacttgt tggatgatcg tcaagccttg ttttgcttca tcgattcctt ggttcattgt
    17521 ctcttggatc gaagcaaaat cgccgtcgat ctctgctagt tgcttaccgg cattttggat
    17581 ctctgggatt ttttcttgca aatcaaggat gatttttgct tgatctttca actcaggcat
    17641 tttgtcattc aatgcaacga ctttttcacc catctcatct actttaggga tgtaatccac
    17701 gaattcattt gctttattca gtttttcctt gatttcaggt agttgacttt gtaattcgag
    17761 gacttgtttt gtgtagccgt cgatcgtgtc caaattttca tctgttgata aaatcatatc
    17821 tttaacttta ttgatactta ctaggttcgt atcgatgtcg taacctatct cattgaatac
    17881 tttcaataaa gtcgaactgg ctgttttgat aaattcattt gtgatttgcg attggatcga
    17941 acttgctcct ttatcggtga ttttcggtgc aatcgcattg attttttcat ttaccgtata
    18001 ttcgattttt ggtttcttga tatcaccact agtaaaactc agcagatctt ctgagaaatc
    18061 ctttggcaag taaatacctg cgtaatactt tccggaacgg actccgtctt ctaattgttc
    18121 ttttgaatca acgaattgcc atcctaattg tttattttta tgaagcgatt cgatgacttg
    18181 ctccccaatg gccacttctt taccttgaaa ctctgctggc ttgtctgcac tgtaaacagc
    18241 aattggcagc tctccagtat ttccatatgg atcccataat gctttgatgt taaaccacgc
    18301 atatagcgaa gggatgatca tgatcgcgat gatcagaagt gttgcaatcg ggtttttgaa
    18361 aatccgtttc cagtcaagca aatataatcg aagcgtattt ttgattgact gcatagttct
    18421 cccccttctt cttttttcag ctgaattact ttaccatgta tttcatactt ttttcaatcg
    18481 gcttttttca aaaaagtcga atttttggac atatcacggc aattgtctca ttgtaatctc
    18541 ttacatattg agaaataatt aaggattaca gcctttactt tatcatgaaa taaaagggaa
    18601 agataatttt atgccaaagt ttttatctat tatacgtata cgcaaaaaag ctaaaacatt
    18661 ttttgtcttt gcccctacaa aaatgtttca gcttttttcg ttttttattc ctgtatttct
    18721 tatttttcga atgacaggat gattttccca gcaggaggat gtccttcaac tagctgatgt
    18781 gcttggatga catgcttaag tgaagcagac agattcattg aaataaaaac atgaaaggct
    18841 cctttttggt aggctttgat tatgccagca aaggcttctg catctagata ttcctttctt
    18901 ggaacaaacg attcataaaa gccttccttt ttttgccgca aatctagatc tgggaggtca
    18961 tttaatgcaa catacctgcc gcctggtttc atgatctgga tgccggtttc tcctttgata
    19021 cttcctttcg tcgcatcgat tacaaggtct gcctgatcag caaattgcag acctggattt
    19081 gttttatcat aagcagcaaa tgctgaagct cctaattttt ttaccttttc ctcattttta
    19141 ctggatgcac tggttaagat tcggattccc ttttcatgga gaagctggat cagacttgac
    19201 ccgaccgcac ctgatgctcc tagtatcatt acagtatctg tcggctggat ttccgtcaaa
    19261 tggttgatta gattataagc cgtaattcct ggcgtgacca ttccggctgc ttcttcccaa
    19321 ctcattttat ctggaatttt tgctactttt gctgatggca acacgacttc ttctccgtaa
    19381 gtcccgccga ctgcatggac agctactcga tcacctacat gaacagttgt gacatctgaa
    19441 ccaatctctg tcacaatacc agcgccatca ttacctggta cataagggaa tttcaatgtt
    19501 cgtatctctt tcatttctcc taaacgcaag gcaacatcat aaggattcac actgaatgct
    19561 tttatttcta cacgaacatg tccagaaccg acttctctgg gatgagcatc gatttcttca
    19621 aaaacatctt gtgcttctcc gtaaccgtta attgcaaatc ttcgcatggt caccatctcc
    19681 ctttttcttt aagtttacgt ttatgccttt attttgacaa aaaaaatgtc ttttcgtttt
    19741 ggcgcatgat tcaaaacaaa atagttgtct tttttcgcgt aacgtgttaa attatttatc
    19801 taaaagcgta aagattacga tttacgctaa taaaaaataa ttggaggaat ttgttgatgg
    19861 ctaaaaaatc aaagattgcc aaagcaaaaa aacagcaaga aacgattgaa cgctatgctg
    19921 ctttacgcca ggaattaaaa gcgcaaaaag attacgaagg cttgcgtaaa cttcctttag
    19981 atgctcgtcc agaacgattg aaaaatcgtg atttaatcga tggacgccct cgcggataca
    20041 tgcgtaaatt cgggatgtcc cgtataaaat tccgtgaatt agcacataaa ggtttgattc
    20101 caggagtaaa aaaagccagc tggtaatcca agtctggctg taatgtaaag gaggagggcg
    20161 catggaccac tccttggatc atcaaaaaag atacgttcgc ttcttctagc agaaatgctg
    20221 gtactagaag cagacgtatc tttttttacg cacagctggt ccttttttat atgtaaaggc
    20281 tgcatggtta cttcgctata tttatttagt aacaggagtc agtgcttcga tacccacact
    20341 aattttcaat gctcggtcaa cttctgccat aatgggtcga tccaaatggc agaccttttc
    20401 tttcaatctt gatttatcga tcgttctgat ctgctccaac aggataacgg agtctctttc
    20461 tattcctgtt ccagtagctt tgatcccaat gtgagtcgga agcttaggtt tagccatttt
    20521 tgccgttatg gccgcaacaa tgatcgtcgg gctaaaatgg ttacctagat tattttgaat
    20581 aaccaaaact gggcgtgttc cgccttgctc cgatcccacg actggagaaa gatcagcaaa
    20641 ataaatatct cctcttttta ccataggatc ctccttattc ttgatattct cttggtatcc
    20701 gctgtcctaa tccgcaagcc acttcatagt ggatcgtacc taattgatct gctatatcct
    20761 gcatagtgat ttctttgtct gcatttttgc cgataagcgt cacttttgtt ccgacaggaa
    20821 attcttgagg caatcggatc atcagttgat ccatacatac acgcccaatc gtttcacagt
    20881 agttcccttc taccaataga gaaaaaccct gcatctttcg tggccagcca tctgcataac
    20941 cgatcggtat cgttccgatc cactctgctt caggagtgat atatgtttct ccatagccga
    21001 ttccttcacc tgctgggagt tttttgactt gaatcaattc agaaaccagc tcaagtgctg
    21061 gctgcaatgg ataaacttcc ggtagtgcgt gcccagaggg attaagtccg tacattgcta
    21121 ctccataacg aatcatattg ccgattgttt catcatgcca taaagccgtt gcactattgc
    21181 tgacatgtac atatcgagga agttcgggaa gttttttcag cacttctata aagcgttctt
    21241 tttgcagatt ccaatatgta tcatctgcct gatctgctgt agaaaaatgg gtaaataaac
    21301 cttcccattc taatacacga ctttctttga tgaatgcagc tgcctctttt acttcttcag
    21361 gtgtacaaaa tccgatacga cccatcccag tgtctgcttt tagatgaatc gaaagaggcg
    21421 tctctagctt acttttctct aaaacagcag ctgctgcttg caaccattcc cgtgtacctg
    21481 cagttactga taaatcatga gcaacagcta cagtcagtgc gtcgtaagga accatattca
    21541 agataaggat gggctgagta aatccagctt ctctcaactc gactccttca tctaaatttg
    21601 aaacacaaaa gcccgaagct ccacctgcaa ctgctgcctc agctgtttca atagctccat
    21661 gaccgtatcc gttcgctttt actactgcaa acaattcttt tccttgcgga agtcttgcaa
    21721 cttcatttgc tacattttca gttatagctt ttttatgtat cactgctttc gttgggcggt
    21781 gccaagcgac aaccatcgtt tttccttctt tctagtcttc caaaataatt tgtgcaaaag
    21841 ctgtttcatt tgtatgagta atactgaccc atacattgcc ttcatgaggt gaacgcgtca
    21901 caacaggctg accattttca ttttttaaaa tctcgacatc ttgaaaggta acttttccga
    21961 tcccagttcc ccaagcttta gagaaagcct ctttgcaagc gtaacgcccg cctaaaaatt
    22021 ctacttggcg atgaaaaggc aaggactgga atagtttcat ttcatcagag gtcaagattc
    22081 gtgcaatgaa ttttggtttt tcttcgatca gccgagtgat cctaggaagt tcgactgcat
    22141 cgatcccaat tccttttatc atatgctttt ctcttttcaa taaatttaga acactctcat
    22201 tctaccaaat tataaccatt ttgccacaaa taagtccaca aataacaaaa atgaaagttt
    22261 ctcttaaaaa agagaaactt tcattttatg tttaatcgtt atttgaacgg attacaaatc
    22321 cgcgtttttt atcattgtga cgtttaccgc catctttttg gctgttatta tttttgctat
    22381 aaccgccttt tgatttgttt ccacgataat taccgccgtc acgacgatta cggttgtttc
    22441 cgccaccacg tttgccattt ttattgtatc cttttttctg cattggcaat gggcgttctg
    22501 gtgtgatctt cactggcact gcatcagctg gatcttttgc tgtcgttttt agcaaaagtg
    22561 caactaaatc ttgtgcagaa tattcttcta ataatttatc tgctgtttgc aggtatttat
    22621 ctagaccatt ttctgcaagt tttgtttcga tttgttcaac tgcagcacct aattgacctt
    22681 tgaatgcttc tttttcggtt ggtggacgta aagtcgtcat ccgttttttc gtcaagtttt
    22741 caatcacatg caagtagtcc atttcatttg gtgtaacgaa agtcacagac ataccgcctt
    22801 ttccggcacg gcctgtacgt ccgatacggt gaacgtagct ttctggatct tgagggatat
    22861 catagttgta cacatgagtg acacctgaaa tatctaatcc acgtgcagca acgtctgtcg
    22921 ctacaaggat atctaaatga ccgctcttaa atgaacgcaa tacgctcatt cgtttttgtt
    22981 gagacaaatc gccatggatt ccttctgcac ggtaaccgcg tgcttctaat ccgcgtgcca
    23041 attcgtctac acggcgtttt gtacgtccga agacgattgt taattcaggt gtttgaacat
    23101 caaacaagcg agtcatgata tcgaattttt cgtattcttt tgcgcggaca tagtattgat
    23161 cgatcaagtc agctgtcatt tctttcgctt tgatcttcac atgatgtgga tttttcatga
    23221 atttaacgcc aatattcttg atagctggcg gcatcgttgc tgagaaaagt aatgtttgtc
    23281 tttgatcagg aacttgtgaa atgatttttt cgatatcttc caagaagccc atattcaaca
    23341 tttcatctgc ttcatccaaa actaatgttt gaacggttcc aagtttcaat gtgtggcggt
    23401 taatgtggtc aagcatacgt cctggtgtac ccacaacgat atgtggacga tctttcaagc
    23461 cacggatctg acggccaata tctgctccgc cgtaaacggc ttgtacacgt acttttttgt
    23521 cgcgccctaa acgatataat tcttcttgtg tttggatagc taattcacgt gttggtgcga
    23581 taactaaacc ttgtaattcg tgacgatctg gatcgatttt ttctaacatt ggtaatccaa
    23641 atgcagctgt tttccctgta cctgtctgtg cttgtccaat aacgtctttt ccagctaatg
    23701 ctagtggaat cgtttctgct tggataggcg ttgcttcttc aaaaccagcg cgttcgactg
    23761 atttcaacaa ttctggtgat aattctagtt ctttaaattt caaatgaatc ctcctatatg
    23821 gttgttaact gaggatgtag cttttttgct accccgtcga tttgttcggt caaagtgtat
    23881 tgtttctttt tgacagtggc cacgcacgtt tttatccatt cttagcccct gtggcaccac
    23941 cgaaaaatca ctttgcgaag tttaggcatt agaaaagcca aacgggtcgc gttcagcttt
    24001 atcattatta tgacatctgg cttcaacaag ttgtttctat ggcaaaacca gtccaatttc
    24061 tcttcatctt tcggcaatag gttattctaa catagttttt cgttttctgc aagtttacag
    24121 atttttattt catttgctga agttgttcta ccacttcaaa taagcccatg ctgttgctgc
    24181 cttttagcat aatactatct gttgcttcaa gattttgttt gatagcatca atcaatttat
    24241 tcttttcttt ttcaaaatag aacacaggta gttgggggta tttttggttc aattcttctt
    24301 ttagtgcatg catttgttcc ccataaagga aaatcatggc gaatttctca ggatcgatat
    24361 gttcactcat ttgcgcatgc atatttaaag actctttccc taattccagc atatctgcta
    24421 acacagcgat tctgcgtcct tttgttggca gatttttaaa agtatctaat accagtccca
    24481 tggctgttgg atttgcattg tagacatcgc tcaaaatagc tgcaccattt cctgccgtta
    24541 gccattcggt tctgttttga gtgatagaaa catgggcgag cccttggcgt atctcaggta
    24601 tggacagtcc aaagaagcgc ccgatcgtat acgcaatcaa agcattcgtt acattataac
    24661 ttccaggcag cgggatcgta aatgtttctc cttctactac aaaatcggtt tgtgcttttc
    24721 cttttgctgt cacttccgca tgaacatcgc catttccaat accgaatgtt gtgactgttt
    24781 gtactaatgt tgccgttaaa ggtgtcagca acggttcatc tgcgggcaca acgagtaatc
    24841 cgtctgatac aagtccgtca gtgatttcca ttttggcttt agcgattcct tctcgcgacc
    24901 caagattttc tatgtgtgct tcaccgatca ttgtgattgc tgcagctttt ggttgagcca
    24961 taagtgaaag ttccgtgatt tcattggcgt gatccatgcc catttccaaa acgatctttt
    25021 ctgtatcttg gggcatgtgc aaaatcgtgt aaggaagacc aatattattg ttgtaatttc
    25081 cttgcgtctt atatgttttg tatttttcag acaagacaga cgcggttaga tctttcgtgg
    25141 tggttttacc attgcttcct gtaattgcaa cgacttctgc gcctactttt tccaagtaat
    25201 aggttgccaa ttgctgcatg gctttcaaag gatctgctac aggaataact ggaaaatttt
    25261 ttggagcttg ttcaacaggc aagctccaaa atgaagcaga tgctccgttt gtgatcgctt
    25321 gctggataaa ttcatgtcca tctcgcgtac cagcaagagg aacgaataaa cttccttcgg
    25381 ttatcttccg gctgtcaaat tcgacattgg taatctttgt cagtgcagag cattcgctgc
    25441 ccacggcttg tgcaatttct tgtattgtta gattcattga gatctctcct aaaaaaggtt
    25501 gtgaaaaaag cgtttggcct aaacggcggg ttcgcatgac tctcacttcc aagtaaattt
    25561 ctctattcgt atttagaagc tagaatgaac gcggttacgc aatcactcca gcctcttctt
    25621 tatttctctt tattcatttt tttcaaaaaa agattgacgc tgatggtatc gattcattcc
    25681 taactggatc agttcttcaa tcaaatcacc gtatttcaag cccatatttt cccataagag
    25741 tgggtacata ctgaactccg taaatcctgg catagagttt aattcattca ggaataattc
    25801 atttttattt gtcaaaaaga aatcgcaccg gctcaatccg cttccaccta acatcgtgta
    25861 agctaacttc gcgtactctt gcgctttttg ataaacttct tctggcactt cggctggaat
    25921 ctgcatttcg attttattat tgatatattt tgcttcataa tcatagaatg ctacgtcttt
    25981 tacgacttca ccaggcaaag tcgtccgaac gtcttcattt cctaatacag caacttcgat
    26041 ttcgcgcgct tcaattcctt gttcaacgat tgctcgagaa tcatactgat aggctgttgc
    26101 taaagcattt tgcagctctt ctcggttttc tgcttttgta atgccgacac tagaacccat
    26161 attcgccggt ttgacaaaca tcggataaag caaagaacct tcacattgat caaatacttt
    26221 tttaggattt tctttccatt gattcttaag tactggtaca taaggaactt gcggcacacc
    26281 agcagcttgt aaaatatact tggtcatgat tttatccatg gcacatgcac tggtcaatac
    26341 gcctgcgccg acataaggca tattcaatgt ctctaagaag ccttggatcg ttccatcttc
    26401 cccgtttggc ccatgtaaaa ctggaaaaac gatggctcct tcttctttga tttcgcccgg
    26461 attgatcact tttcctgtaa agccttcctc tgtctgtcca cttgggtccc atgaaagatg
    26521 caagacatct ttgctggcag gtttttctgt tagtaatgga cctttgaccc attgtccttc
    26581 ttttgtaata aatacgagtt gaacttggta ataattataa taaatggcat ttaaaactga
    26641 aaatgcggaa agaatggaca cttcatgctc tgcgctgcgt ccgccatata gtaaagtaat
    26701 cttcaaaaga ttgtcctcct gtattctaaa tcgttcagtg atttactctc atattttagc
    26761 acaaaacaaa aataaacgaa taacttattt ataaaaaaac gagccagctt ctaaaagaac
    26821 gagcgtcatt tattttttgt cagctagatt atttgtcccg ttttttataa aaagttatga
    26881 taaagaaaag gagggatgat gatggcgcaa acaaatccac gcagccgctt ggcagaaatc
    26941 attcgagtgt tcatcaatta caatgtagtg ccaaactttg tacagcaaaa aaatcccgaa
    27001 caggtaaaaa aagcctttga agaactaggc cccaccttca tcaagatagg acagatgctt
    27061 tctgttcgag aagatttgct ttcttctgcc ttcactcaaa cattcaaaac attacaagat
    27121 agtgtaccta gcgacacatt ttcaacggtc aaaaagacga tcgaaacaga actttctctt
    27181 tcattatcag atatattcga tgatttttca aaatcgcctt ttgcctctgc ttctatgggg
    27241 caggctcatc gtgccaagct gaagaacggt gacgcggtag tcgttaagat ccaacaccct
    27301 aacatcgccg aagagatccg acttgatctt caactatttg aacgagcgat ccctttgatc
    27361 aaatacattc ctgaaactag cgtagttgat ctaaaagggg tactccaaga agtaaaacgt
    27421 tccttgatca atgaaatgga cttcttaaaa gaaagtcaaa atggcgaaca attctatcaa
    27481 aagaacaatg gctggaaaga gatccgcagc cccaagattt atgacgcttt ttgttcgaaa
    27541 aaagtgatcg tgatggaaga aatgtctggt aaaaacttga atcatttaat gaacgccgaa
    27601 aacaaaactg aaacttttat cactggaatt caaaacaagc agctgaaaca agaagttgca
    27661 aagcttttag ttgagaactt tatgaaacag gtgtttgatg atggtttttt ccatgctgat
    27721 cctcatccag ggaatctgct attccatgta ctaacaaaag aagaacagac acaagctagc
    27781 cgaaaaactg aaacagtcca tgaaaaagag ttcggttcct ttgcttttcg agcctccact
    27841 tctgcagaag accctgttgc gccatatacc atcaactaca tcgattttgg tatgatgggg
    27901 catctttctg caggacttag gcaaaaactc acacaagccg ttttagcgct gtatacgaaa
    27961 gatgcttatc gaatcgaaaa agcggtcctt cgactttgtc agcaagaagg ctcgtttgat
    28021 gaaagtcggt tccaccaaga actgacaagt tttcttgaac agtattatga ttctcctatt
    28081 gatgaaatca acttgcaaga agtattcaca catgtcgtga cgatttgtca tcaaaataat
    28141 ctgcagttcg atcgtgatat cacgcttttg ctgaaagcat ttgggacatt agaaggggtg
    28201 atccgcgtcc tcgatcctga agtttccctc atggaagtcg cttcgccttt tgctcaacat
    28261 tattttctca ctcatctgga tgtagaagat acattgaaac aaagcggatt agacttattg
    28321 gaagggatga aggctgcgcc taagatccct caacagctcc accatctatt agaaatgtgg
    28381 acttcgggac aaggaaaagt caatttggaa ttgaaaaagc aagacaaatt attgagcagg
    28441 atcgaaagta tgatcaatcg gcttgttttc ggtatgattt tagctgcatt gatcgtaggg
    28501 tcctccctgc ttgtccaagc agcacctgta gaaaacgcag aggtcgtttc cttattaggg
    28561 atatttacgt atgctattgc cgcattcgtc attatttttt tggcaatcga tgcattgatc
    28621 caaatgtata agaagaggaa aaagtagaaa aaggttgtga cagaaagaat cagcttcaag
    28681 aaataagaag gggttttaca taaattgttt ctcaaatttt tgtgaaaatt cggcttattt
    28741 ttcgaagagg ctacttctga aacaccgaga ataaaaaatg tgaaaaagct gttctgcacc
    28801 cagtaataag aacaatcaaa aagggaacat ggaacacatt tatcctgttt ccctgttccc
    28861 tttttgatgt attcgagcat aaataaaata ccttcgttac ctactatctt ctgtacttgg
    28921 taaaccttag tcaattgcct tttttaacgc ttgacgaaaa gacatatgat ctttctccgt
    28981 aaagggtttt ggaccctttg tccttatgcc agcttttctt aactttccac tatgataacg
    29041 ttgttctaga agcgtgccga tattcgcttc gtcaaattct tttccagagt gatggaacac
    29101 tcgcacaccc ttcggcaata caagtgaagc taaaccataa tcttgcgtga tcaaaaagtc
    29161 attcttctta accataccga cgattttata atccgcacta tcagctccgc ggtctacgta
    29221 gacgatttgg acatgattag cgtattcttt ggtcgtataa tggtccacac tggtcacgat
    29281 aaccacgtca agaccatagg attctcctaa ttttatgaca tcttctttta ctggagaacc
    29341 atctccatca atgaatagcc tcattctttt tcgccttctt ccataaatga taaacagtta
    29401 ctaatccgct cagtaaaaga ccaacattga tccattttgc caagataagc agttgtacac
    29461 tttctatcgg cactgattca tggttcagcg tgatcaataa tagatggatt ggctctccca
    29521 tttcttttgc taaaacaaac aaaagcgtca tcaataaaaa ctggtggatc acactccctt
    29581 tattttgagt agtatacttg acaacgatcc ggcgatccat tggaatagct gtatcagttt
    29641 gataatattc cttatttagc cagctgcctg ttactacgtg ccagaaacta agcagaaaga
    29701 cacctatcgc aaaaccattt ggaatcaaga ggaccaataa agaagaactc agtccaagca
    29761 attgcatagc caatggagac catgttaaac gtttcaaaat ccatggtccg actatttttg
    29821 ctgcaatcaa gcctgctgca taaggcaaat acataaaaat cgaaagtttt tctaatccgt
    29881 acagtatgcc aacataaaaa ctgctaaata aaaaaaggaa attccctagc attccattta
    29941 gaaaagtaaa cagatttagc cagcttggca acttccattc cttcttctgg tgtcctaaaa
    30001 caaacacgaa caagatgaac agcagcaaga aaccataaat agcaagatca aaaagccttt
    30061 catcgaataa caatcgggca tttctcaaaa gaaacaataa gccaaagaat atcaaaaata
    30121 aaacaagctc tcgcgtagct accagatttc ttttgatgtc tttgaaatcc agttcgtaac
    30181 gcggataatg actaaccgta tggtaggcca tgacgtaaaa aagtgtataa atagtaaagc
    30241 ttgctgcaat ctgagtcggt gcaggcaact caatcgcttg atacaggaga aatagccaga
    30301 tagctgcaaa taagtacttt ttcccatcca tattgataaa accttgcgtc ttttcgtgaa
    30361 aattgaccgt ggtattagca ggtacaagcc atgctgcact cagccctagc aaaacacttg
    30421 aaaaataaaa taaaggaaaa taaaatacgc ctaatattcc aagcaatgaa ccagcaccac
    30481 caatcagcag agcgatcatc agcaacgtgt aactatctag accaaatcgt agactgttga
    30541 ctaaaaatag tccagtcatc cgaaaggcat aaaacagcgt aaatggcaaa atcgtcagcc
    30601 aattatgcga gtctgttagt cccaaaaata aaagataagg aataaaaata accgtgttac
    30661 ttaagataaa gggcgttgtt tgtgtaaaat tacgcccaaa tcgtttgagt ttttcgaaca
    30721 tgacaatggc tccttaaaca gtcttataac taatctgctc ttcgatttta gcattaaagg
    30781 ggaaataaac caattttaca gaatatattc ttgtaaaaaa aaatacagaa agaagcataa
    30841 gcttctttct gcatttttac ggtacttgat tactcaagca gacaagccct tctccacaaa
    30901 agtcttgccc gacacccctg catccggcag gtgataaaga tagaaatcaa ttatcctgtg
    30961 cctccaaaat caattaggag ctctgtaaac cagagtcctt ctcgacccgt cgcataaatt
    31021 tactttaaca cagttcctat gtttatgcga gagctttttt cattttataa tcgaccattt
    31081 gaaatccttg accgatatct gaaataagtt cttctgtaat ctcaaatcct tcatgcttat
    31141 aaacctcgat tgcttgatga ttcccctgat tcactcgcaa aaagatctct ctttcctttg
    31201 tttgcgcagc aagttcttca taccagcgaa aaactgaaga agtcaatcct tgaccacgta
    31261 ctttatttag caaatataat ttacttaaat aaatttttcc atttatctct tcataagcag
    31321 tgtaaccgac agtcttttct tcgcttttta ataaaaagta ggacacacct tctgtcagtt
    31381 ctttttgaat ctgttttttt gactgataag ttttcagcat gtaatccact tgtttttgac
    31441 caatgatcgg cacaaaaact tccggccata cttcttgtat gactgccatc agctcatcca
    31501 gttcattttc tgttactttt attttttcca tacacccacg tcctttttta cattataaat
    31561 gaccttgcgc ttgatggaat aaaaagagca tgatcatgct atacacaccc accgaccacg
    31621 gtttcaagaa aatcacaatc tgcataaatt ccctaaaaga taatttcgtc aaaccagcta
    31681 ctagacagat caagtcgtct ggcgcaaagg gaagcagcaa ggtaacaact agtacttttt
    31741 taatatcttt cgtttttctt gtcagcaatt catcaaattt ctgatacttt tttggcgata
    31801 aaataagctg gacaaaggct acgccatagt aacggacaag caaaaatccg attgcttccc
    31861 caatcactag gccaatacaa ctatataaaa gcccaaatcc attgccaaac atcagcatgc
    31921 ccgctactga ggagatccct cccggcaaaa taggaatgat cacttggatc acttgcaaca
    31981 gaacaaatat catcactgca tacttgccaa attgctggat aaacgcttgc aaagacgtgc
    32041 tcgatcgaaa aatcccatga tgataagcat aaagaatcaa cacaaagaaa aaaaacagac
    32101 caataatggg catgatctga agacattttt gtaaaactga tcctttttta gacatctata
    32161 ttccccacat cttttttcat ttattaaaac tgcctcctt
//
LOCUS       DARBDV010000023        32065 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000023 DARBDV010000000
VERSION     DARBDV010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.023"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(269..802)
                     /locus_tag="SCM56_001021"
     CDS             complement(269..802)
                     /locus_tag="SCM56_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HEG1109837.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(1666..2418)
                     /gene="xth"
                     /locus_tag="SCM56_001022"
     CDS             complement(1666..2418)
                     /gene="xth"
                     /locus_tag="SCM56_001022"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEG1109838.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            2531..3451
                     /locus_tag="SCM56_001023"
     CDS             2531..3451
                     /locus_tag="SCM56_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG1109839.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            3524..4459
                     /gene="murB"
                     /locus_tag="SCM56_001024"
     CDS             3524..4459
                     /gene="murB"
                     /locus_tag="SCM56_001024"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEG1109840.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            4757..6235
                     /locus_tag="SCM56_001025"
     CDS             4757..6235
                     /locus_tag="SCM56_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1109841.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            6512..8572
                     /locus_tag="SCM56_001026"
     CDS             6512..8572
                     /locus_tag="SCM56_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEG1109842.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            8655..9224
                     /locus_tag="SCM56_001027"
     CDS             8655..9224
                     /locus_tag="SCM56_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1109843.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(9399..10205)
                     /locus_tag="SCM56_001028"
     CDS             complement(9399..10205)
                     /locus_tag="SCM56_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109844.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            10343..11857
                     /locus_tag="SCM56_001029"
     CDS             10343..11857
                     /locus_tag="SCM56_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HEG1109845.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(12260..13243)
                     /locus_tag="SCM56_001030"
     CDS             complement(12260..13243)
                     /locus_tag="SCM56_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEG1109846.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(13248..13895)
                     /locus_tag="SCM56_001031"
     CDS             complement(13248..13895)
                     /locus_tag="SCM56_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG1109847.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(13980..14612)
                     /locus_tag="SCM56_001032"
     CDS             complement(13980..14612)
                     /locus_tag="SCM56_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEG1109848.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(14628..15179)
                     /gene="coaC"
                     /locus_tag="SCM56_001033"
     CDS             complement(14628..15179)
                     /gene="coaC"
                     /locus_tag="SCM56_001033"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEG1109849.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(15176..15913)
                     /locus_tag="SCM56_001034"
     CDS             complement(15176..15913)
                     /locus_tag="SCM56_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEG1109850.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(15910..16899)
                     /locus_tag="SCM56_001035"
     CDS             complement(15910..16899)
                     /locus_tag="SCM56_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEG1109851.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            17157..17699
                     /locus_tag="SCM56_001036"
     CDS             17157..17699
                     /locus_tag="SCM56_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1109852.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            17716..18801
                     /locus_tag="SCM56_001037"
     CDS             17716..18801
                     /locus_tag="SCM56_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1109853.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            18813..19619
                     /locus_tag="SCM56_001038"
     CDS             18813..19619
                     /locus_tag="SCM56_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1109854.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            19620..20447
                     /locus_tag="SCM56_001039"
     CDS             19620..20447
                     /locus_tag="SCM56_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1109855.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            20444..21517
                     /locus_tag="SCM56_001040"
     CDS             20444..21517
                     /locus_tag="SCM56_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1109856.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            21945..24641
                     /locus_tag="SCM56_001041"
     CDS             21945..24641
                     /locus_tag="SCM56_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG1109857.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            24718..25206
                     /locus_tag="SCM56_001042"
     CDS             24718..25206
                     /locus_tag="SCM56_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1109858.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            25218..25712
                     /locus_tag="SCM56_001043"
     CDS             25218..25712
                     /locus_tag="SCM56_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1109859.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            25733..26542
                     /locus_tag="SCM56_001044"
     CDS             25733..26542
                     /locus_tag="SCM56_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1109860.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            26539..27360
                     /locus_tag="SCM56_001045"
     CDS             26539..27360
                     /locus_tag="SCM56_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG1109861.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            27411..29510
                     /locus_tag="SCM56_001046"
     CDS             27411..29510
                     /locus_tag="SCM56_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HEG1109862.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            29507..30625
                     /locus_tag="SCM56_001047"
     CDS             29507..30625
                     /locus_tag="SCM56_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HEG1109863.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            30664..31542
                     /locus_tag="SCM56_001048"
     CDS             30664..31542
                     /locus_tag="SCM56_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG1109864.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            31733..31885
                     /locus_tag="SCM56_001049"
     CDS             31733..31885
                     /locus_tag="SCM56_001049"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HEG1109865.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT        10308 a         5523 c         6635 g         9599 t
ORIGIN      
        1 ggtaaacggt gtccaaaagc ttgcatctgc ggtaataagt ccaaccaaat gaaaaatatg
       61 agaagctatt tttgtttggc tgttcttatt acttgatgca gaacagcttt ttcacaacct
      121 ctccaataaa cggtgttctc ctgccagctc ctcggtcacc agtcacaata tttaataaac
      181 atgagaagct attttttgaa attgctcctt gtgactcgga gtgaaacggc aggctcacga
      241 cctctttgtg tgtttcataa gtattctttt actttatagt cacaagtttt ttcaatggat
      301 ctgtcccgaa ttttttttct tgataaagca ggatttccgc acatgcacga ctatcttcca
      361 atgcatcatg gtgatgatct aattggatgt tcaaattttc gcacacagtg ttcaatcgat
      421 gatttggaaa ttcaggaaac aggcgacggc tacttttcac tgtacataaa gaaagataat
      481 taggctgttc aagatgataa taatctaagc atcccgctaa gacctttgta tcaaaagcag
      541 cattatgtgc aacgactaaa cgattttttt gaaaatatgg ttggatagtt tgccagactt
      601 ctgggaattt cggcgcattg cgtacgtctt cttgatggat tccgtgaatt tggacatttc
      661 gccagaaaaa aggagtctca ggctgtatca atgtatagaa ttcatcaacg atctggctat
      721 tttttaccat gactagcgca agagaacaag cactataagg ctgatgattg gcagtttcaa
      781 aatccatggc aacaaaattc atttctgtaa aacctccttg ggaaattact gtaacatact
      841 tttgaaaaaa gcaaataaat ttaacaaaaa acatagaatt ttttatgtaa tatttgttga
      901 gaaaagctag tttttgaact cattccttgt gatttggagt tagatgggga tcatgacttc
      961 tcggtattaa gtcaaattta tgcaaagttg tgaggaacca ttttcaaaaa aatttcttac
     1021 gactcgaaag taggcgactt tttcacaact tctccactaa acggtgttca atcagctgac
     1081 cctcggtatt agctcaaaaa atagcaaaat atgaggaact attttgccat ttgtttctct
     1141 aatactcagg tcaggacgct gatttcacaa cttctcaata aacggtgttc aaaagcttgc
     1201 atctgcggta ataagtccaa ccaaacgaaa aatatgagga actatttttg tttggctgtt
     1261 cttattactt gatgcagtgc agctttttca caacctctta ataaacggtg ttcaaaagct
     1321 tgcatctgcg gtaataagcc caaccaaacg aaaaatatga ggaactattt ttgtttggct
     1381 gttcttatta cttgatgcag tgcagctttt tcacaacctc ttaataaacg gtgttcaaaa
     1441 gcttgtatct gcggtaataa gcccaaccaa acgaaaaata tgaggaacta tttttgtttg
     1501 gctgttctta ttacttgatg caggacagct ttttcacaat ctctcgataa acagtgttca
     1561 aaagccagtt tctcggtatt atgccaaatt tatgcaaaat gtggaaagcc attttcacaa
     1621 aatttttctt aatactcgaa actaaacgac tttttcacaa cctctttatt tcaaatctaa
     1681 ttctactgga caatgatcac tgcccataat gtccgtatgg atctttgcat cagctagtcc
     1741 tggctctaaa tctttgctcg tcaaaaagta atcgatccgc caaccagcat tgtttttcct
     1801 tgaattgaat cggtagttcc accaagagta gacaccttcc tgggttggat aaaaataacg
     1861 gaatgtatcg ataaacccgt tatccaacag tcgagaaaga gccgctcttt cttctggagt
     1921 gaatccagca tttttttgat tcgttttcca gtttttcaaa tcaatctttt gatgagcgac
     1981 atttagatcc ccacacacga tgactggttt ttgttttttt agattttcca aataattata
     2041 aaaggcttct tcccattcta atcggtaatc taaacgtttt aattcgcttt gagaatttgg
     2101 cgtgtaacag gttacaagaa agaaatcgga atattccaaa gtaatcagac ggccttctgt
     2161 atcatggata tcgataccca ttccgtaagt ggcattcaat gctggttttt tagcaaaaat
     2221 cgctgtgccg gaatagcctt ttcgttccgc gtaattccaa tattgataat aacctggaag
     2281 atctaaatca atttgtcctg cttggagttt cgtttcctgc agacagaaaa aatcggcgtc
     2341 taactcatga aaaacttcta aaaaattttt atttacgatg gcacgaagcc cgtttacgtt
     2401 ccaagagata aatttcaata aaagcacact ccttcgaatt aatttcctta cctttgcgat
     2461 actattgtac ctcattttag taaaaaatgg tgtaagatga aagtaactaa atgatccagg
     2521 aggaaagtag atgaagattg gtgtaatcgg tgtagggaat atcgcagaaa aagcatattt
     2581 accaacttat gcgaaaaaac aaggaacact tgatttttat tttgcaacaa gaaataaaga
     2641 aacaaaagaa agaattcaaa aagagtacgg gttccaacat ctatatgaaa cgatcgatga
     2701 gcttttagaa gaaaaaatcg aagcatgtat gatccacgca gcaacaaaag tacattatga
     2761 attagccaaa aaatgtctag aaaatggtat ccatgtttat attgacaaac cactgagtgt
     2821 ccatctaaat gaagttcgtg aattgcaaga attagctgat aagaaccaaa taattttgat
     2881 gatcggtttc aaccggcgct ttgctcctat ggtagaagaa ttgaaacgta tcccagataa
     2941 gcggttgatc cacctgcaaa aaaatcgaat agcggcaaaa gaaacgacag agttcgtcct
     3001 ttatgatttg tttttacatc ttattgatac agctgtgtat ttattagatg aacccatttt
     3061 gagaacaaaa taccagataa gagaaacaaa ggaatttttg gaatatgcca ttttacaatt
     3121 agaaacacaa aatcaaacgg ctattttaac gatggattta gcaagtggcg ctaatataga
     3181 aaaatatcaa gtaacaagta aaaatggtac ttatgaagta aacgatctga cccagcttgt
     3241 cattcaagag gctgatggaa aaaaagtgaa agaatttggc gactggacaa acacgctagt
     3301 caaacgcggg tttgagccaa tggtagaggc cttcttttct cagatccgtt taggtaaatc
     3361 ggaaacagaa ttgcttaaac aaaaagaagt tgtaagaagt catgaattat gtgaggaagt
     3421 gcttaaagat cactaccgtc atcaactttg aacaagactg gcaagattgt attaagcgtg
     3481 atagaataat gaggacgttt gagaaaagaa aggaaaatcc tcagtgaata aagaagaaat
     3541 agttgaaagc ttagccacga ttactatatt gtttgatgaa ccattgatga actatacgtt
     3601 tacgaaaaca ggtggaccag ctgatgtcct tgcattcccg aaaaaacaag aagaagtaaa
     3661 gcaaatcatc gattattgcc gaatccatga tataccttgg atggtattag gaaatgcaag
     3721 caatctgatc gttcaagatg gcgggattcg cggtgtagtc atcatgctga ctgaaatgaa
     3781 acagattcat gtcaaaggaa cgatggtcat tgcggaagca ggtgcctcac tgattgatac
     3841 gacttatgca gctttggcag aatcattgac tggatttgaa tttgcttgcg gcatcccggg
     3901 aagtgtcggt ggagcggttt atatgaatgc aggtgcgtat ggcggagaga tcaaagatgt
     3961 ttttgctgaa gtggatcttt tgcttgaaga tggaacattg aaaactttga caaaagacga
     4021 aatggtattt tcttatcgtc acagtaaagt ccaagaatta cgtgctatcg tattggaagc
     4081 tcgattcagt ttgcaaacag gggattatga agcaatcaaa gcaagaatgg atgagttgac
     4141 cgaattgcgt caatcaaaac agccattaga atacccttca tgcggaagtg tctttaagcg
     4201 cccagtagga cactatacag gtcagctgat ccaacaagca ggattgcaag gcctgaaatg
     4261 gggcggtgca caagtctctg aaaaacatgc tggattcatc gtcaatatcg atcatgcgac
     4321 agcgacagat tatattgaat tgattgcgca catccaagaa gtaatcaaag aaaaatttga
     4381 tgtgtcttta gaaacagaag tgcggatcat tggagaaaaa caagcaatat cagaaacaag
     4441 cagaagttta gccaattaat aagtaatgga acttaaaata agtaaaagag caggacggtg
     4501 gccgtcctgc tcttttttgt ggttgtttac gcaaatggcg caaatcttgt acattttaac
     4561 acaataaaac aaaaaatata gaatatttcc tcataaattt ttccattttt tgcaatattc
     4621 tcaaaaaaaa gataaaaaat gagaataact gatgaaaaaa caagcataat ccatcataaa
     4681 atcagtatag gtgaaaagat gaactttcat ctgcatatca agtaattggt atgaggcatt
     4741 ttttgaggtg taggatatga agttggatta ccttatagaa gaggaagata gagaaaaact
     4801 gctgctagct agatatttag aatttgagaa aaagaattat gtagtgctgc ctaagttata
     4861 tgaaacatta ggtttgtcta aatttaaaac aagaaattat atcatagaat taaataaaga
     4921 atttactgaa ttcaaaacaa agccagagat ccaaatttca gaagaagcag agatccatgt
     4981 cgaacgtctg aatttgacgg atatcaaaaa tttaagaaga atctactttc aacgatcaaa
     5041 aattgctcga ctgttaacgg agatcgtgga ttcaaaagta acgatcgaaa aatttgcaga
     5101 gaatcatttt ttaagcatat caagagccta tgtaaaaaga agagaattag tctcttttct
     5161 tgaacagcat caaataaagt taaagaaaaa catattagtt ggggaagaaa taaacatacg
     5221 gaacgtatta tttggggtat atttcggggt ctttaatggt ttttctatgc catttagaga
     5281 aagcgtcatg aatgaagcaa agagtatcgt gcaatatttt gaatatctgt tcaatttaaa
     5341 actgtctgca acaaaacgaa tcaaattcaa tttgctaacc actattagtt tgttgagggc
     5401 ctatgggaat caaccaatcg gaaaacgttt cttttcagat gaatacttta agagctcaga
     5461 aggaatgaag atcatcaagg atttatcaca tatttctttt cttcatatcc ctaaagagca
     5521 gatcattgat gagatttctt atatcatgtt gtttttgatc acggaagaag aatttgaatt
     5581 agatggcgca tttggacttg cggaagctca ttttcatgat atccacatga ttagtcaaaa
     5641 aatgtcagtg gaattgattg atcaattacc attttcgatt gaaatctctg aagaagaaag
     5701 actatatata cgaaaagaac ttcgtatggg tatgatgcat gtcaatcgaa aaaattggat
     5761 attcgacttt gagatgacta gctttacgtc taaaaagcag ctacagtttt tcttcgagac
     5821 gtatccaata ttcagtacag caatccaaaa ggtcatacaa gagaataaac gctatttctt
     5881 gggaaaagat gaacgtttca tgattcgctt attttatgat tatctctttt tattggtcgg
     5941 tatttgtccg gtatcatatt ttgaagcacc gattcatgtc tgtattgatt tttcgcaagg
     6001 atctgcttat accgattaca ttatttctca agtgaaagga tttaaaaatt acaatattgt
     6061 gattgagagt cgaatcactt ctcgtacgaa tatttattta tctgattgtt tacttgaaaa
     6121 atttaataaa gaacagataa tttggaaaaa tccaccgact ccagatgact gggaatattt
     6181 tggaaatacc atcatcagag aaaaaaatag acatttaact gaaaaaatca gctagtattt
     6241 atggatggca gctttgtctc acatatttat tagctgattt atgttttttt caaagaaaag
     6301 gtaactagat ataactagat ataactagat atattactta acaaaattaa aaagtacata
     6361 agttattatt ccgattttct tgaaacaata aaaaatcaaa aaaaatactc tcctaatagt
     6421 ccatcttctt ttgattttat ttatataatg aaatcatctt atgaaaatta ctacttcatg
     6481 tttagacaag agtagggagg actaagaata aatgtgttca aaaagacgtt caaaacaact
     6541 aaataaacac gcttgtcgtt tagataagga acggtcgtgt tctgaaaaac actacgttaa
     6601 gtttatgatg cgcacgatgc tgatcagttt atctgtaggt gtctatatgc atgtacccgt
     6661 tcaggcaacg acacaaacca tcaaggtcgt acgtacctct gtttctgaga ccactgattc
     6721 ccctatcact attactcata ctttcactga tgcaaatgga aaacagctac ttcctgcatt
     6781 gacttcctat aataaagtca gttatcctcc aacgataaac cactactggt taattcctaa
     6841 caaaacatta tggacatcgg aaaccggaag cagtgtgccg ctttctatct tttccgatat
     6901 gactgatcct acaaaaggtg atcagttaaa acagatcact gatattttga atcatgcttt
     6961 gacagatata aaggacggtg gaagcgcaca agtttcttat gtatatgaaa aagattattc
     7021 acagtttgaa agaaagaaaa tcgatgttct ggcagggaca aaagtttcga tatctgatct
     7081 ggtagaaagg ctgaagaacg cagatggaac acaaggagat atgtccaaag taatttcaaa
     7141 caatgggaat atcgtggata cgactaatac aggaacatct actatttcct tgtcctattt
     7201 tgatccaatt gctttaaaaa caatgactgc atctgctgtg atccatgttc gtgctgatca
     7261 aacagctatc aaaggaaaag atcttcactt aagaacagga gagagttacg cccctaaaga
     7321 tcttgttgaa tatgtaacaa aaagcgatgg aacttcagga aataaagaag aaatcaaaat
     7381 gaacgggaca gtagatacta caactccggg agaatatccc gttgtattaa cttatataga
     7441 ctcgaaaaca tcaaatgtgg cagaaacaac agctgtagtt tatgtagaag catctgctgc
     7501 ttcatcgact gtcatcactc attttataga tagtcaaaca aataaagaac ttcaagcaga
     7561 aacacatatg agtagtgtaa atcgtattcc tgatttgcgc ctacctgctg aattttctga
     7621 tcgagtgcca aatatagatt tatcgactta tgaacaaaat gggaaagtca cttctatttc
     7681 tgattggatg caagcaaacg gattagacag aaaaagtcac tggtcggaga tccttgaagt
     7741 acttcactca gaactgatga ataataggaa gcaatcagga ggggaaatta cctttactta
     7801 tgtttatata ccagacaatc gttcattaga aggtcatgat cttattgttc atccaaacga
     7861 aaaaataaac aaagaagagt tgatccaatc agcaacaaat tccttaggta aacccgtacc
     7921 gttaaaggat gtatcggtta caatcaatga agtaccattg gaggatggtt atttgaccaa
     7981 acaaacaggt gattttacga ttatgtatag ttatcatgac ccgtattcac tccaagaact
     8041 ccatgctcag tcactattac atgttttacc attaaacaaa cagatagatc aaaaagaaca
     8101 aaaaagacga gaagaaagct tgtcagaaaa aaaacaacaa atggctcatg cagtttctcc
     8161 aacagggaaa gtaaaacaaa caagaagcct agagttagca agaatatcta gaaaaatctc
     8221 agaatctaat aaaacgataa ggcaagaagc tcatttggcc ccacaagttt ctggattgga
     8281 aaatgaaatt ttagagccta aaataaaaca acaatcagaa aaagaatttg ttgctaaaca
     8341 gtcggaagat cagaaggaat cagatgacca gaaggaaacg aagaaaaaac gcttgtcaac
     8401 tattgattcc accgatgagc atctgcagcc gcaagtacca cttcctagtc cgggaacacc
     8461 cgcacctggt ggaggtattc cttcttctgc tttaggagat ttcatgcgag gtatggcagg
     8521 aacatgggta tacgctaatc gaaatgaatt tgacgcagat gactggttct gatttatatc
     8581 tctatctgtt atagtcaaat aagtagctga gtcatatgtt atttggcata atggagacga
     8641 ggagggatag aaaaatgtta gataaaacgt taccttacgc agaaatctgg atgacaagac
     8701 cacttcaaca aaatgttcca atcacgcctt tagcggaagg ttttgaattt cgattttatc
     8761 aaaaagaaga tgaatctgct tgggcggaga ttgagacttc cgttggggaa tttcaaacga
     8821 cagatgaagc acttgagtat tttcaacaaa actttgctcc ttatcctgaa gaacttgaaa
     8881 gaaggatgtt ttttgtagag acaaaaaaag gagaaaaagt agcaacctgt accgcttggc
     8941 gaaaaaaatc aagagagcaa gtgtatccgg tttttcattg gttagcagtg aaaccagatt
     9001 accaaggaaa agggctcgca aaagcattga ctgccagagt cttgcaagaa tttcctaccc
     9061 tccatacgga cggaccaatc tatcttcaca cacagacatg gagccatcag gcgattgctc
     9121 tttataaaaa aatgggtttt acttttattg ctgaaaatct agacggcaca cctaatccag
     9181 attatgaaaa agttatgcag gttttaagta aattagataa ataacgaaat ggtgaaataa
     9241 gtcctcagta ttgggataaa aaacgaaata gtcattcctc atatcttact tttttgagtt
     9301 aatactatcg gataactaat ggcatgccat acattcaaaa aagaaggggc agtgacaaaa
     9361 gtattgtcac tgccccttct cttgtttttt agcatgtttt atcgtttgat ttttttagga
     9421 ataaaattct tgatgaacgt aaatttttgg taatcgtttc gactatagag gtaaccaaat
     9481 acactgaaaa caactgctcc aataaaatta acaaataaat ccttcatagt atccatgatt
     9541 cccacgtcta agtaaccatt ttggatgatt gttttatctg ttttaccatc tgcattttga
     9601 ctttcaatga ttgtccgagt gatatctgga acatgataga cagtgtttcc gtctttactt
     9661 aactccacac tattgatttg ctggatgatc gtatcttttt gcatatcgaa ttgcagtaat
     9721 ttatctgctg aaaattccac aaactcccac ataacaccaa ctgtcatcga aaaacagaag
     9781 gaaacaactg ctagtaataa aggagtcaag ttcatggttt ttgtattttt attgagcaaa
     9841 taaacaaggg aaaagccgac acccccagct aaaaatccgc tgaatgcatg tagaaaagta
     9901 tcccacattg aaaaatagcc ataaaaatca cttaactctc ctaaaactgt tgaactaaaa
     9961 ataaaagata acaaaatgat ttctagcata tttggaatct gtatctttaa tatcttttcc
    10021 agaaaataag ggatttcaaa taaaattaat gtcagtaata aaagcattgt atttccccag
    10081 tgttggttcc agacctgatc cactagcata ataacgacga tgatacgtaa taaaatatag
    10141 attccgtttg tgattctacc tgcagttttt gtactgttcg taaaaaaggt tttcaaatga
    10201 ttcataaaat aactccttca tgtatatcct taattatact aagaagcgtg tgtttaccaa
    10261 agctatatga cttaaagaaa aacagagttg atattgtgaa aaaaatcact taatatagta
    10321 taaggagaaa agggggatac aaatgaaaaa attagttgct ggtttattaa cagccgttgc
    10381 acttcttgct atgagcgcat gcggcgcaga tcagaaaaat acagcgcaga caagttctag
    10441 tacgaaggaa tctgccgaag tatcatcagg cgcttctgaa caagtatata cagatccaaa
    10501 agaaatgaaa gaatcttatg atgtcgtgat tattggagct ggtggtgcag ggatggcagc
    10561 agctatcgaa gcaaaagatg ctgggttgaa tccagtaatt ttggaaaaga tgcctgttgc
    10621 cggaggaaac acatctaaat catcaagcgg tatgaatgct tcagaaacaa aatttcaaaa
    10681 agaagaagga atcaaagact cgaatgatgc cttctatgaa gaaacgctaa aaggtggtgg
    10741 aggaacgaat gataaagaaa tgcttcgata ctttgtcgat cactctgctg atgcggtcga
    10801 ttggctagat caaaacggta tcaaactgga caacttaacg atcactggag ggatgagtga
    10861 aaagaggaca catcgtccgt ctgatggctc ggctattggt ggatacttag tggaggggtt
    10921 attgaataac gtccatgagc gggaaatccc tatttttgtc aacgcagatg tgacagacat
    10981 taagaaaaat ggggaaacgg tcaatgaagt gactgtacat gttcaaggag aagaaccaaa
    11041 agaggtcacc ggaaaagcaa tcgtagttac cacaggcggt tttggtgcca gcaaagaatt
    11101 tattgaaaaa tatcgtcccg atttagctga ttatattacg acaaatcaag aaggatcaac
    11161 tggagatggt atcaccatga ttgaaaaatt aggtggacaa accgttgata tggataaaat
    11221 acaaattcat ccaactgtgc agcaggatga gggggtcttg attggtgagg ttgttcgcgg
    11281 agaaggagca atcttggtcg atcaagaggg gaaaagattt gtcaatgaaa tggatacaag
    11341 ggataaagta tctgcagcaa ttacagcatt gcctgaaaaa tcagcttatt tgatttttga
    11401 ccaaggcgtg cgtgatcgtg ccacagctat tgaattttat gaccaaaaag gatatgtcac
    11461 aactggaaag acaatggaag aattagcaga aaaaatcgat ttaccaaaag aaacactagc
    11521 aaaaacagtt gatggatgga atacggatgt tgcggagaaa aaagatgctc aattcaaccg
    11581 tgccacagcc atggaacatg acttatcgtc tccgaattat tatgcgatca aaatcgcacc
    11641 aggaatccat tacacgatgg gcggtgtgaa aatcaatacg aacactgaag tattagataa
    11701 agaaaaccag ccaattaggg ggttatatgc tgctggtgaa gtcacaggtg gcttacatgg
    11761 aaacaaccgg atcggtggaa attcagttgc ggagatcatt atttttgggc gacaagcggg
    11821 catacaagca gctcgatttg cttcggaaaa tgaatgaaat agaacgtaaa taaaaagagg
    11881 ttgtgaaatc agcgttttga gctaataccg agggtcagct gattgaacac tgtttagtga
    11941 agaggttgtg aaaaagctgc cctgcatcaa gtaataagaa cagccaagta aaaacagctt
    12001 cttatgtttt taacttggtt ggacttatta ccgcagatgc aagcttttga acaccgttta
    12061 ttcggaaaaa gagcgtggga caaaagtaaa gaacactttt gtctcacgct ctaaacacgt
    12121 ataaacggcg gaagcagaag aaattccttc ggaaataagc tgaaattcac aaaaatttga
    12181 aaagcaattt tcgtgaattt cttcttattt cttggagtta aacgcttctg tcccagcctc
    12241 ttttttgatc agcatgattt tattcagtta tcttcaaaat gatttttccg atgtttttgt
    12301 ttgcttccat atattcatgc gcttgtttga ctgcgctgaa ttggaatgta cggtcaatga
    12361 ttggccgaat cttgccattt tctagaaaag gcccagcttt ttgcataaaa gtttgtgcga
    12421 gctctgcttt atactgatca cttctaggtg tcaataatgt accaagcagt tggacacgtt
    12481 tcccaatcaa tacaccaagg tctatctcag gcacgatggt cccacctagc ataccaatca
    12541 agacccaacg tccatcaacg gctatagaag ctaaattctt ttcccagtaa gaagcgccga
    12601 tgaaatccaa aataacatcg actccccgac cttcagtgat ttttcgtacc tcttcttgaa
    12661 agtcttgttt tttatagttg atcaattcat ctgcaccaag ttctttacaa aaggcgagtt
    12721 tttcttctga tcctgcagta acgatgattt tcgcgtgaga aagctgtcta gctaattgga
    12781 tcgctgctgt tcccacacca cttgctcctg catggatcaa gacagtttcg ccttcttgta
    12841 attttccaag ccagtaaagt gtttgataag cagtcaaaaa aacttctgag attccagcag
    12901 cagaaacata gtctagcgta tcagggagaa ggattgcacg atcggcaggc atcacagcat
    12961 attcggcata accacctaga ttgactaatc cgtatacttt tgtcccagga gtaaaagtgg
    13021 agatatcact tttattctca actacctctc cagctacttc gactcccaaa atagggtagg
    13081 gaggtttcaa agagactgat tctcttttca taatatctgt tcgattgaca gctgctgtat
    13141 ggactttgat cagtaattcg ccttgttttg gtttaggaac aggcacttca atttgttcta
    13201 atacatctac agaaccagga tgcttgattg aaatggcacg catggatcta ttcctccttt
    13261 gcccattctt ggaatgctgt attcaactct ttgctacctt cagaaagtac tagagtcgta
    13321 cgagaaatat ccgtttcttc cactagagcg gcaagtacag cagcaactgt agagcgagtg
    13381 atcgtttttg atgccttctc attaggctta gttaattgaa tggaagtaac ttcttcatcg
    13441 ttagtcaatg tgactggttg aagaatgaca aaatctaagt tagttcgttt cagccattca
    13501 tctgcataat gtttagtaat ataataatct gtcatggact caggccattt tgctggttca
    13561 tctgcataaa cagcactgac catcagatag cgagagacat gcgcattttc agctgcgatc
    13621 actgttttga ttgcgccatc caagtctact tgcagaagat tctttccttg agagccggca
    13681 gcaaaaatca atacatcgat gcctttgaat gcttcggtca ttttttccac ggaccaagtt
    13741 aaatcaaaag agacatacga aacattctct ttttttacta atgtttggga agcaacatct
    13801 ctcacccctg caaaaatttc atgggaactt ggagctaaat cttttatcag gtgtcggcca
    13861 atttgtccgt ttgcacctac aacaaaaatt ttcatttgga tcatcctttc gacgtcaaat
    13921 ctttccattc aatagtaaca ccaaaagaga aaaaaatcag agatcggcta cgatgactat
    13981 cagttagtaa gagaagggtg cttgaagtat cttaacaaga cattgcatac tgctgcagtc
    14041 gcaagacctg cgacgattcc ttcaggtata ccacttgtaa aaataaccgc tagaatagct
    14101 gaataaacag catttaaatt tgttccgatg actttggcat aatcttgctg gaataaaaag
    14161 tagatcatat tcatcactaa aatcgtattg gtcaatgaac cggaaagtcc agctaagaat
    14221 aaagaaacgg aatctgcttt ttgtttcatt agtttgcgca gacctttata aacgaaataa
    14281 ggaaccaccc caatcaagat gcgaggaata aacgcgatca gcaaggcttt ccagcttcca
    14341 tgttctgttc cgatgactgg gataaacgga gaaaagacaa aagacaaagg tgtttggata
    14401 actgtgctac ggatcatgct gatcagtcca aatgtaccgc caagaaaagc acctagtctt
    14461 ggtcctaaaa cgatcgaagc aatgattaca ggaatatgca tggttgttgc attgattgga
    14521 ccgataggga taaaacctaa aggcgtaaca gctaataaaa tcaaaatagc taaaaacatc
    14581 gcagttaatg taaagttttt tgtatttttc ataagtactc cttttttcta agcatttctt
    14641 tcattaagag tcagcatgac ctgttcgatg atcgtctgaa tatcagctaa ggcacctttt
    14701 ccaaaatctc cacaagctaa aagagattct cttggttcaa tttcctgata gccgacagat
    14761 ctcaatgttt ccagattacg ctgagtgatc gggttttgat acatattcgt gttcatggca
    14821 ggggcaatca attttggtgt tcccactgga agcgccattg cgactgttga aagaagatcg
    14881 tctgcaatgc cgtttgctag cttgccgata atgtttgctg tagcaggcgc gatgagaaac
    14941 aagtcagctt gttttgctag ttcgatatga ttgattacct gcggatttga ttcttgcatg
    15001 acgtctgtat ggactgcgcg cttcgacaga gattgcaagg ttaatggcgt gataaaatgt
    15061 gtgctgcttt tagacataat cacatctacc tgggcaccta atttgacgaa ttgacttgta
    15121 agatcagcgc ttttgtaagc agaaatactt ccagtaacgc ctagtaagat ctttttcatt
    15181 ttgattcctc cagtctaatt ttctcagtaa tcaatgcagc aatctcagat ttactttgag
    15241 cttcttccac tgttccatct ttagataaga gatagccgtg gtgtgtctcg ccatggattt
    15301 ccgtcaaatc atttgcgaaa ataaaatcag ttttgttatt tatcagacta ttttttgcga
    15361 ctgccagaag agattccttt ggtacatcta ctaatagttt gaaaccaatg atttttgctt
    15421 ttggctgcca ttcacgtaga taagaaatga tttttggtgt tttcttcaaa acgagcacaa
    15481 gatgctcggt gttagaagag atctttgaaa cagtggtatc tgtctgttca ttttccgcaa
    15541 accaattgtc cagttcttct tgcgttgaag aagtgggtag gttttttgcc agctgttctt
    15601 ctgaaaaact aaaagcagga gtaaaatcac tgactgccat actgtggata atcgcatcgt
    15661 agtgttcttt tttcgttagt tcttctaatt gtaaaaataa gtccagtgca gattcgatcg
    15721 tataaatcgt tatattggag tgtcttttcg gtttcagtgc ttgtttcgta gtcacgtaat
    15781 cgattgtggt atccggattg gcagctaaat aatcagctaa tgcttgccct aatcgtcctg
    15841 tcgaatgatt tgtaatagca cgtacttgat cgattttttc agtcgttcct ccagctgtga
    15901 ttagaatctt catttatggt cacctacatt tactagtgtt cttccttggt gtgttccagc
    15961 aagtagttta gatgctgttt caggcaattg atccagagta atctcttgga cgaccagttc
    16021 atctgcaatc tgcaaatccg ccatctgttg ccataaagaa agacgttgat ctattggtac
    16081 attgacagaa tcaattccaa tcaactggat gtttcgcaga ataaaaggca agacagtcgc
    16141 gtttagtttg attcccccag cattgccaca taaagctact gcaccgttat aagagatcaa
    16201 tggaagaaga ctgcttagct gctcgccacc tactgtgtcg atgacatagt cgatttgctg
    16261 ttttcctaaa gcttttgttg tctctggcag aaattcatca ggtcgtacga cttgcaaagc
    16321 acctagactt tttagccatg tgacagactc ttcttttctg gaaaaggcaa tgattgactg
    16381 gaatcctagt ttatgtaaca gggctatcgc tgtactgcct acaccgccag aagcaccagt
    16441 aacgacaact tttttatcgg aggtcatacc ttgacggatt aaagcgttca cacacaacat
    16501 agcagtaaac cctgctgtac caaggatcat cgcttgacgc agcgtcatat tttttggtag
    16561 aggaactaac cagtctcctg gtactttttg atattggcta taaccgcctg gatgggtgac
    16621 gccaagccca aaaccagtag caagtacaag atcttctttt gaaaaacgtg gattgttact
    16681 ttggacgaca gttccggata aatcaatccc tggagtcatc ggatatgttc gtatcacctg
    16741 accagactcg ctcattgcca atgcatcttt ataattgaca tcagaatagg caatttttac
    16801 caagacttca ttttcagcca actcagtaaa tggcagatca ctgattgctg tatggaaaac
    16861 aggttccttt attacctgaa aagatcgaaa agcgttcatt cgtgagacac tccttttaaa
    16921 attttctttt atcataccat aaagcctact cttttattct tgttcttccg taaatttaga
    16981 caaaaattag gtgaaaaata aagaatcaaa caactagaat ttattccaaa agataaagaa
    17041 atattcagca aaaaaaattt tacaaagggt tgcctttttc aataaatttc gtatactaat
    17101 acgagtggta gtaaacagga agtttactat tagaaaactt cgattcggag gaaattatgg
    17161 aaattggtga gaagttaaga aatttgcgca tccaaaagaa tttgacacaa gaagaactag
    17221 gcgaacgcac agatttgtca aaagggtata tttcccagtt ggaacgagat ttaagttcac
    17281 catcaatgga aacattcttt tctattcttg aagtcttagg tgtgactcca gaagaatttt
    17341 ttcatcaaga agcagcgaat ctgcaagtgg tttattcaaa agaagatcat acggtttatt
    17401 tcgatgaaga aaacgggtat gaattggaat ggctcgtggc tgattcgaat gaaaaagaaa
    17461 tggagcctgt cttgctgact tttgaaggtg caggagaata taagacgttt gaaccttcat
    17521 tatctgaaac gtttattttt gtattagaag gggaattaga gttaagctta ggagagacgg
    17581 tctatgtggc aaaagcagga cagtcgatct attatcaagc gacaaaacaa catcaattaa
    17641 ggaatcatct caaaaaacga acaaaagtat tgattgttgc aacgcaatca tatttgtaat
    17701 atagggggta aaaacgtggg gaaaccaatc atttcatttg atcaagtagt gaaacgctat
    17761 gatgatgaaa cagtattaaa aaaagtaagt tttgagatcg aacaaggaaa attttataca
    17821 ctgcttggtc cttccggctg tggtaaaaca acgatcctac gtattatagc tggattcacc
    17881 gaagctacag aaggggacat ctattttgaa gggaaacggt tgaatgattt gccagctaat
    17941 aagcgacaag taaatacagt cttccaagat tatgcacttt ttcctcatat gaatgttttt
    18001 gataacgttg cttttggatt gaagatcaag aaaatgtcca aatcagatat cgaaaagaag
    18061 gtaaaagatg ctttgcgtat ggtccagctt cctggatacg aaaaacggga gattagtgaa
    18121 atgtctggtg gtcaaagaca gcgtgtagcg attgcacgag caattgtaaa tgaaccgaaa
    18181 gtattgctat tagatgaacc gttatcggcg cttgatttga aactacgtac agatatgcag
    18241 tatgaactgc gggatctgca acaacgatta ggaatcacgt ttatttttgt tactcatgac
    18301 caagaagaag cgttggcaat gagtgatgag atttttgtca tgaataaagg gaaaatcgtc
    18361 caaagtggta cacccgtaga tatttatgat gagccaatca atcatttcgt tgctgatttt
    18421 gttggggaga gcaatatcgt ggacggtgta atgatcgaag ataatctagt tgagtttgtt
    18481 ggtaagcaat ttgaatgtgt cgatggcggg atgcgtctaa atgaaccagt tgaagttgtt
    18541 ttacgcccag aggatttgac gatcacgaca ccagataaag gaaaactagt cgtaacggtc
    18601 gatactcagc tgtttcgtgg tgtccattat gaaatcatct gttatgatga acagcaaaat
    18661 gaatggatgg tccattcaac gaaaaaagct aaagaaggaa gcaaagtagg attggccttt
    18721 gagccagaag atatccatgt catgcgtttc aacgagtctg aagaagaatt cgatgcacgt
    18781 cttgacagct acgaagaata ggggggacaa gaatgaaaac aatgcgcagg atctattccg
    18841 tcccttatat tttgtggctc ggtttattcg tgatcgcgcc tgtattaatg atcatttatc
    18901 aatctttttt tgatatgaat gggcaattca cgctgaataa ttatctatct tattttacgt
    18961 cggggacatc gctttctatg acgatcaatt ctgtatggta tgcatttttg atcacgtttt
    19021 tcacgctgat catcagttac ccgactgctt attttttaac aaaactgaag cacaagcaat
    19081 tatggctgat gctgattatt ttgcctacat gggtgaatct gctgcttaaa gcttacgcat
    19141 ttattgggat tttcagtgtg cacggcagcg tgaatcattt ctttgatttt atcgggatcg
    19201 ggccgaaaca gatattattc acagacttca gttttctgtt cgtggcaacg tatatcgaga
    19261 ttccatttat gattatgcca attttcaatg cattggaaga gatgaatcct tctttgatca
    19321 gtgctagtag agatttaggc gcgaatagct ttgagacctt ccgacgagtg atttttccgt
    19381 tatcattaaa tggtgtgaaa agtggtgtcc aagcagtatt tattccttct ctttctttat
    19441 tcatgctgac tcgtctgatt ggcggaaatc gggtgatcac tttaggaacg gctatcgaac
    19501 agcatttctt ggttacacaa aactggggga tgggttctac aatcggtgtg attttaatca
    19561 ttgctatgtt catcgtgatg ctcctgacag gcgagaagaa aaaaggaggt cgctcataaa
    19621 tgaaaaaatt taaatggtcg tatctttatt tagggatcgt ctttttattc ttatatgcac
    19681 cgatcttcta tctgattttt tattccttca atgaaggagg aacgatgaac agctttacag
    19741 gatttacttg ggaaaattat gaagctgttt ttgaagatac acgtttgatc attatcgtat
    19801 tgaatacgtt catgctggct ttcttatctg cattgatcgc cacgatcatc gggacatttg
    19861 gggctatggg aatctattac acgaaaaaac gacgtgcacg gacagcgttg ttgagcatga
    19921 ataacatctt attagtctct cctgatgtta ttatcggtgc tagtttcttg attttcttta
    19981 cactactagg attcggatta ggttttcaga gtgtattgct ttcccatatt gctttcagca
    20041 ttccaatcgt cgtcttgatg gtcttgccaa aattgcagga aatgaacgat tcaatggtcg
    20101 atgcggctag agaccttggt gctaataatt tccaagtagt caaaaatatc attttgcctt
    20161 atctttctcc aggaatcata gctggatatt ttatggcatt tacctattct cttgatgatt
    20221 ttgcggttac atttttcgtc acaggaaatg gctttacgac gctctcagta gagatctatt
    20281 ctcgtgcaag acaaggaatc agtttagaaa tcaatgcgct aagtgcgtta gttttcttgt
    20341 tctccatgat attggtcatt ggatactact ttattagtaa agaaaaccgt ccaaaacggc
    20401 tgaagaaaaa cagaagagta cgcgaggggg tggcacggct gcgatgaaaa aattacaatc
    20461 tctgttttta ggtattctca tcattattgc ggtgctgctg cttggtgttc gtcaacttga
    20521 aaaaacaagt ggcatgagtg gagcaaaggt acttaccatc tataactggg gagattatat
    20581 cgatcctagt ttgatcacga aatttgaaaa agaatatgga tacaaagtga attatgaaac
    20641 atttgattct aatgaagcga tgttcacaaa aatccagcaa ggtgggacaa actacgacat
    20701 cgctatacca agtgaatata tgatccaaaa aatgatcaaa gaaaaactag tacttccatt
    20761 agatcattcg aagatcaaag gattaaataa tattgatcca cgatttttag acttggattt
    20821 tgatccagat aatcagtatt cgattccata tttttgggga actttaggga tcgtatataa
    20881 cgataaattc tttgatgcaa atcaaattaa gcattgggat gatttgtgga agccggaatt
    20941 aaaagacagt ttgatgctga ttgacggtgc acgagaagtt atggggcttt ctttgaatag
    21001 tttaggctat tctttgaata gtaagaatat ggcacagttg acagaagcag caaataaatt
    21061 gaatcattta acaccaaatg tcaaagcaat cgtggcagat gagatcaaaa tgtatatgat
    21121 caatgaagaa gcctccgtgg cagtgacatt ttctggcgaa gctgccgata tgatgtggga
    21181 aaatgaacat ttacattatg tgatcccacc agaaggatcc aatctttggt ttgataatat
    21241 cgttatgcca aaaaccgtga aaaataaaga aggtgcctat gatttcatca actttatgct
    21301 agaaccggaa aatgcggcac aaaatgcaga gtatattggt tattctacac caaatgaaga
    21361 agcaaaagcg ttattgccaa aagacatttc gtcggacgaa caattctatc catctgatga
    21421 cacgatctcc catcttgaag tctacaaaga tctaggatcg aaatacctag gcatctataa
    21481 tgacctattc ttagaattca agatgtatag gagataatgg caaaactcag taagaatggg
    21541 gacttatcaa cgttctaaag agctaaaaat cgtccttttt ctatctgagt tttccaatct
    21601 tctaccaaaa gcggatgaaa cgccgctttt tttgtgttat tttatttttt ctaccacgtt
    21661 tttctaccag agaaaagtgg acaaaaccat tgtaaaatag ggcccgagac gggctagtaa
    21721 atctaagatt gaaaaagtta cagaaggata aagtaattgg aaactatgca aatatactta
    21781 acccttctgt aatctattaa taaaactaat atatgaatcc agttcaatat ttgtagctat
    21841 gaaaaaacat aaaaatcata ttagataaaa actaaattgg tagataaatt attttgtcag
    21901 atatctgagg caaatattcg actatttgtg tttcaaagta aaaattggaa tacaaatata
    21961 gaatagacat attgcagccg atagtttatt atgcttttag acacattaaa ctggaggaaa
    22021 aaatgaaaaa gagcgctaaa gatcgagttt ttgatgagct gatacgacta acagggaata
    22081 tgtcttctgt cactgccgag aaaatttcga agaatctagg tatctctcgt cagaatgcca
    22141 gccattattt gacgagatta gtggaagata aaaaggtcga gaaattaccg ggaaagcctg
    22201 ttttgtggaa gcctttagat gaatatgcgg ttgtagataa tacggagcaa ataaacgaag
    22261 cctttcattc tgtagttggt catgacggaa gtctgagaga ggtcattcaa aaatgtatag
    22321 ccgctgtcaa atatccgcct aacggactca gtgtattaat aaatggagcc acgggtgttg
    22381 gaaaaagttt tttagccaca aaaatttttg aatacgctat ccatgagcaa attattgaaa
    22441 aagatgcacc atttgctatc ttaaactgtg ccgattatgc ggacaatccc gaactgcttt
    22501 cggcaacact gtttggatac aagaaggggg cctttacggg ggctgaaaag gataccgatg
    22561 gtttattggc aacggccaac aacggctatc tttttctcga tgaggtccat cgcctatcga
    22621 aagaaaatca agaaaagctc tttctattta ttgatactgg aaactatcgt ccagtcggtg
    22681 aaaatgtaaa ttggcattct gctaaggttc gttttatttt cgccacgacg gaaaaaggtg
    22741 aaaattattt attagacacc tttgatcggc gtattcaaat tagtgttatg ttgccaactt
    22801 ttgaagatcg ccccattcga gaacgattag agctgattca attatttttt caaaatgaag
    22861 cggatgtttt acaaaaagat attattgttt caagagaagc cttgtcttta atgttatctc
    22921 atccctttag tgggaatatt ggtaagctga agaatatcat taaaatcagt tgtgcggatg
    22981 tgtatagtcg aacacaagaa gaaccattaa cgattgggaa aaatgagatc ttgattcagt
    23041 taaatatgct ggaatccgaa gtagaaagct tgccgattgc tagtttattg ataaagaaaa
    23101 atcaaaagat aaaaacggat cgaatcaaac cgtttgatta tgattcgatt ttggaaaaaa
    23161 ttactcagct gataaatgaa ggtaatttca atgaaaattt ttcttcgata aagaatgaag
    23221 tacagcattt ggtgcatcac attgataagg attctttttt atcaatgagt agaaaattga
    23281 tgattcagat gtttagaaag gtctgggtat ccgttttagg aaaaacgtac ggattgacga
    23341 cgagtggacc tgttgcagaa attattagca gatcctatgt catttcccca agctataaga
    23401 cagacttaac cgggataaat cgtacattag ctgcaaatct acctcggacg acttacttat
    23461 gtaatcagtt cttggagcat ttaccaccgc tctcagaaaa ggacgagaac tttctaagac
    23521 tgcttattgt cgtgacttta tctgattatg tagatgaatc catcgagctg aagggcctgt
    23581 tgcttgctca cggagatagt acggctagca gcattcaagc agtagtgaat caattatgcg
    23641 gcaattatgt ttttgaagcg ttggatatgc cagtagagag taatgtgtca gagattgtcg
    23701 aatcgacaag gaatttcgtg aaacggcaag tgaatacaga tagtctagtg ctgatcgtag
    23761 atatgggctc tttaaatcaa atttacacac agatcaagaa cgatttaaaa ggggagttac
    23821 ttcttctgaa taatttgacg acttccattg cacttgatat tggcttgaag atgacgatga
    23881 atgcgccatt taaacagatt gcggaggatg cagcagacaa gtattcaatc aatgttcaat
    23941 actttgaagg cttttctcaa agtagcaaca ttatcatttc ctgtatgtcg ggtttgggga
    24001 tttcggacaa attgaaagat gtttttcgtc atcatttatc ggatgaatcc atcgaagttt
    24061 tcacgaagga ctacagagaa ctacgtgaat taatcgacca aaatgatgaa acttattttg
    24121 acaagacaaa attggtcatc acgacttcgg atcttccacg ttccttttca attcctaata
    24181 ttaatgttta tgacatctta gacggtgatg gagcagccaa tttggagcat attttgagtc
    24241 ctgaaatgaa gccggcagaa tttaaccgct tgattcagga gctagtaaaa ttcttttcgt
    24301 tggaaggaat tgcggaccgt ttgaactttc tgaacccaac gatcgtgatg agtgaagtgg
    24361 aaacagtcat ttctaaatat gaaaattact atcatttcac tttaaatggc cgtgtaaaac
    24421 taaatctcta tatgcatacg gcattgatga tggaacgttt attcttggct cggacagaaa
    24481 aatctgaaga accgaattat gatttgaaac cagatgaaga tgaattttat tatgtttcta
    24541 gaagtatttt tcaatcaatg gagatgaaat ataattttaa agtcaatgct tatgagcttt
    24601 cgctgctcta tgaattatta ggaccgtata ttcaaaaata aagtgtctaa aaaagtgtct
    24661 aatctgtttt ttttaggtta gacacttttt ttaatgtgaa ttaacgtgat taaaccaatg
    24721 tttttaaaag ttggcacagg aattgcttat ataaaagtga acagaaagaa aggaattaac
    24781 catatgaaga aaattctaat agcgactcac ggtcacttag cttcaggctt tcttagcagt
    24841 attcaattac tgacggggaa ggttcaagag attacggtga ttaatgctta tgtggatgag
    24901 tgtgattttg aacaggagtt gaatgtattt gtaaaccagg tgaacgagca ggatcagatt
    24961 tttgttttta ccgatttatt tggcggaagt gtcaatcaga agattaccaa aacctttttg
    25021 gagaaagaga ttgctgtaac tattttgtct ggctttaatc tgccaattct gctcgaaata
    25081 gtattggcaa ccaaggaatt aacaaaggag gatattcagg aactggtaaa taaatgccaa
    25141 caggaattaa agatgaccat tcttgatgag gtacatgtag taaatgaaga ggaagatttt
    25201 ttttaggagg aagaaaaatg attaaagcag tgcgaattga tgaacgatta attcatggac
    25261 aagtagcgat gacttggaca aagagtctaa atttaaccgg gttagtagta gcaagcgatg
    25321 aggcggctac caacgatatt caaaagatga cattgaagat ggctgcacca tcgaatgtca
    25381 aagtcattat taaaacagtt gatgacgcga ttcatttgtt aaaagatccg cgggcgaagg
    25441 acatgcgctt gatggtttta gtccctaatg tcaaagatgc ggtaaaagtt gcgaaagctt
    25501 tcccaacgga aatcgaatta atgaacgtgg ggaatgctgg gaaaatgaca ccgccatcgg
    25561 aagataagaa aaccttatca aaggaagtga tgttaacgac gtcagaaatt caagacttaa
    25621 aagaattagt tgaactttat ccagataccg tttttcagcc tacaccggcg atggaaaaac
    25681 gttcagctat ctctattttg agtgattttt agaaaaagga aggagaaaaa ttatgtatga
    25741 tgcatttgta gtcgctttgg ccgtttttat tggaattgct ggacatgaaa tgttcgggat
    25801 gtccatgctg agtcgtccga ttgttgttgc tccgttagca ggagcgttga tgggggattt
    25861 gcagacgggg ttgattgtcg gggcgtcact agaagccatc tttatgggtg tcgtcaatat
    25921 cggaatctct agtacggcgg agccttcttt ggctgctggg ctggctacag cgttcgcaat
    25981 caaaatggga ggaaacgtgg atgcgattat tcccattgtc tttccactag cagtattagg
    26041 attacagtta atgaatatga ttttttcttt tgtatgtgga ccgatggctg ctaaattttt
    26101 caaatttgca aaatctggtg atcaaaaagg aatcatcaat ctgcacttct ttatgtggtt
    26161 tgtccactat gcgctttatg ctcttattcc attctttgcc gttttatttg gagcagatgt
    26221 tgtcaaaagc gtcttggata atattccaga ggtcatcatg aacggattaa cagtcgctgg
    26281 aaatctgctg ccggcagttg ggatggcgat gctgttacgg atgctttgga gtaaaaatat
    26341 tgctgtgtgg tttttctttg gggccgttgt tatggcgtat ctcgagttac cattgattgc
    26401 aattgccgca gttggaacga ttattgcaat tgttatagcc caaagagatc tacaactaaa
    26461 aaatatatct gttcatgcag ttcaaagcga tgctgctgta aatgcaacgc ttgaccaaga
    26521 agaggaggat ttctttcaat gaaattagct gatggaattt ctaaagatga gaaaaaattg
    26581 gttcggaaga tgttttggcg ctcgatgact atgtatgctt cggtgaatcc ggtgacgatg
    26641 ggcggcggtg gtttcgccta ttctatgatg cccttcatta atcatttcta caaaagtgaa
    26701 gaagatcgag ctgcggcgct ggaacgtcat acacagtatt tttcgacgac cattccttgc
    26761 gcgtcctttg tcatggggat tgcggcatcg atggagaagg aaaattctga aaaagctggt
    26821 tttgacgtgg aaagtatcaa cgcgatcaaa acgagtttga tgggaccatt ggctggaatt
    26881 ggcgattcat tgttctgggg agtttggcgt gtcgttaccg cgggactagc gatcaactta
    26941 gccaatgccg gaaatatttt ggcaccaatt atctttttag ttatgttcaa tatacctaat
    27001 tacttgatgc gatactatgg cggcgtttta ggctattcat taggctctaa atatattgaa
    27061 aagctgtatg cgaacgggtt aattgaaatt ctgacaaaag ctgctagtat cttgggtctg
    27121 atcatggtag gtgcaatgac gatcagtatg gtcaaattca ataccgtttt gagcttttca
    27181 atgaagggcg aagaactaat gaatgtccaa gaaatcctcg atagtatttt taaaggaatt
    27241 gtaccgttat tggtaacatt gagctgtttc aaattactag ataaaaaagt cggcgtcatc
    27301 aaaattattt ttggaatggt cgtcttggga attttattgt cattacttgg aatcgcatag
    27361 taggaagctg cagctactag gctgcagttt ttttagcaag gagggaaaag atgactatta
    27421 atgaagtaat ttattcaccc aaacacccat tttttaatgc tgagttaaac gacggaaatg
    27481 tgactacttc aaacgaatac agctactatc ggaatattcc tgatgtaaag ggattgacct
    27541 tcgatgtaag taatcaaaag acattagcaa accttgatgt ttgcgggacg attcaaacgt
    27601 tgacctttta tcgagaaaat ttactaactg aggaaaaacc aggtgtttgg gtcaataaac
    27661 agctctctca aaccaattcg ttatccttaa aggttgaagt aaacgggatt gtctaccatt
    27721 tgtcagaatc ggatcatcgt attgaagtcg atgtgattca agactgtttg cctagatata
    27781 ttcatcatta ttcgacgttt aaggtcattg tagtatcctt tgctcccata ctggctaata
    27841 agcgtttaag tatgcttgtt caacaggttt acattgtgaa tgaaacacac gaaagccttc
    27901 aagtttcagt gaaagatgtg ccttcgtatc aagagaagta ttcggatcag caaaatgtgt
    27961 tgatttctca aaaaggaaat aaggacacga tcgcacaaca ggaagtggca agcttcgcca
    28021 ttgcttttgt ggacccaaat gcttttgaag aagtaatgac ttttcaggaa tctgatatcg
    28081 aaaaatggct gaaggaaaca ttgaactatt ttgctcagct atttgggcag ctgtcaatgc
    28141 caaacgatag agttgtccat ctctatcgtc gtgcacttta tcaaagcttt tctagtttcg
    28201 gtatgaatcg acagggacag gtcgtcggca gcaattgggg cagctatcct gctactaatc
    28261 gaatttggaa taaggatatg tattacgcat cattgccatt caccatgttt gaaccggagt
    28321 tatgtcaaaa aacggttcta tggtttgatc aatatggcgt taagtttcct ggttcaaaat
    28381 ttacaggagg tatcaatcat tctctgagta attcgttatc cagtgcgtta ctggctagtc
    28441 tttattttga acatactggg gatacaaaat tctttgagcg gtgtccgcag gttctactga
    28501 atgcttcaag aattattgag gacatattag ctcaacgtcg gtcaggggaa ccaatgcttt
    28561 ttccatctgt ttggttatca gacgcctttg cacttggaaa gtatcatacc ggcagcaatc
    28621 tttgtctttg gaaggcctgt attggacttg cagaaatttt tgaagcccta gaacaactga
    28681 gtcttagcaa gcgatacaaa aacattgcct gtaaactaaa ggaatcaatt tgtaaacaaa
    28741 tgactatcga tggtcctttt ggtgagcaat ttttggaggg aatcggtgat gaggagaaaa
    28801 caacctattc agtgaagcac tatcaaaaac cgattcttga gcaagggtta atctttttaa
    28861 gtgatgtgat tgtggatgga aaaattaatt tattgatgca tgatggagaa gaatcagaca
    28921 ctacgttgat gcctttttat caatttttag ataataaaga tcatttatat cagaacacca
    28981 tggccttttc tgctagttct ttcaatccaa cctatagcga ggaaatcaaa ggcatcactt
    29041 ggggattaga atccggcgcc acttttcctg gctttatcac tgttttaatg agcgagctgc
    29101 acaaccctca agctttcgta acccgattag aagaactaat ttgtttggca gatttagacg
    29161 gttcgtggtg gtggtggccg tatcgcttag aggctcaaca aggagaagtc gtgcgagact
    29221 ttggctgcgg caaatgcggc tgggcatcgg gtctttttgt cagcctaatg atcagtcaat
    29281 atttcggatt gaaattcaaa aaaggagtat tgcaaattga tcctgttgat aatttaacgt
    29341 tttcttggaa gaatttgaag ttcggtcaag ccttcatttc gattgaatgt acgcaatccg
    29401 agttgagtat ttcaaatttg tcagaaaagc cattgaaaat aagtgatacg aaaaaaattc
    29461 ttcacgtaga aaagggaaaa acgattcaca ttcaaaggag aaaaagatga gtatactaaa
    29521 aaatattacc caatttaaaa aaatccagag tcgagcaatc actcccgaaa atccaacagg
    29581 cgagaaaggt aaagctgcga tgttgccaag cgagctagga ccaagccgca aaggtcgtgg
    29641 cgcaattccg ctgacttctg gtgaagaaac tgtgattgcg gatattactg gaacgggaat
    29701 aattcgtcat atgtggatga cgattcgtga tcgaacagaa tttggcagtt ttgttttacg
    29761 tgatttaatc ttaagaattt attgggatgg gtcggatagc cctgctgttg aaagtccttt
    29821 aggggatttc ttttgtaatg ggtttggaga gcgttgcgag gtgaactcgt tgccgatagt
    29881 ggttaatcct acagggggct ttaattccta tttcgaaatg cctttccaaa catccgctaa
    29941 aattacgatc gagaatttac atccgaagga tatgtcctca tttttttata cgataaatta
    30001 cgctttggtg gacgaattgg aagaaaatag cctatatttt cacgcaaatt ggcatcggga
    30061 acgcattacc aaattacagg aggattataa gttaattgat cgattaaagg gtcaaggcta
    30121 ttacgtgggt acttaccttg cgcttacctg cctagaacgt tattggtggg gcgaaggcga
    30181 gtttaaattc tacattgatg gggatagtga gtatccgacc gtaacctcaa caggttcaga
    30241 agattatttt ggcggggcgt gggcctttca tgagtacgat gagttcggac gtccacatgc
    30301 gaaaaactat tcaacagcct ttttagggta tccttaccaa tcaaatagag atgccacaag
    30361 agatcgtttt gaaacgggaa aattgaatgc cgttcatgcc tttggtgacg acgcaatgcc
    30421 acgacatggt ctttatcgtt ggcatcttca agaccccatc tgttttaccg aagatttatc
    30481 ggtcacttta caacaaatcg gcaacgatga tgttcgctta tttgagcggc aagacgatgt
    30541 tgctagtgtc gcttactggt attcaacaga agcttcagga aatactaaac gtcatttcac
    30601 tgatcgagaa cgcttgccgc gttaatgaag tgtttttcac atattcgaag ggaggaacct
    30661 atgatgatat atggggcaat tgaagctgga gggactaaat ttgtctgtgc agtcggtact
    30721 gaggaattaa cgattttgga aagagtcagt tttcccacaa caactccagt tgaaacaatg
    30781 aagcaggtca ttgatttttt taaacctttt aaggaacaat tggcaggcat cggggtcggc
    30841 tgctttggtc caattgatat tcaatgcaaa tcaacgagtt tcggtcatat cacatcaaca
    30901 ccaaaacttg cttggcaaaa tttcaatttt gtaggaacgc taaagcaata tttcaatatt
    30961 ccgattgaat ggacaacaga tgtcaatgcg gcttgctatg gtgaatatgt tgctggttct
    31021 ggcaaagggt tagcaggtgt tgcatacttc actgtgggaa caggtatcgg aggtggtgcc
    31081 ttgttcaatg ggactttcgt ggaagggttc agtcatccgg aaatggggca tatgttgatc
    31141 aagcgacatc caaaggacaa tttttcagga aactgtccct tccaccaaga ttgtttagag
    31201 gggatggcag caggaccagc gattgaaaaa cggttgggtg taaaaggcca gaatttattg
    31261 gctgacgatt cgttttggca gattgaagcc ttttaccttg cccaatgtgc atacaataca
    31321 acactgatgt tttcgccaga tagaattatt tttggcggtg gtgtaatgaa acaagaacac
    31381 atgaagaaaa aagttcagga taaatttgtt gagcttataa atggatatgt tgaaatacca
    31441 ccaattgata gctacattat tacgcccgaa ttaggtgata atgccggaat aatcggcgga
    31501 ttagcattgg ccagaaaagc ggtaagaaat aagcaaccat aattaatgaa tgtttcattg
    31561 atactcatgt caaaagtatt taaccgagta atcaaatgat caaatgaagt tggataaagc
    31621 gaaaatgaat tcaaataaaa aggctctaag tcaaatcgtg ttgttttcat taaatcactg
    31681 aaaggaaatg cttctgcgtt tccttttttc tttttgttgt gcgctgtata ccttgaggat
    31741 attcactggt ggcgtacact taaatagtaa tatattgtgt tactttatgt ctgtggtaat
    31801 agttatagtt gcttaccaat gtatgttcat ttttcgaaat gttgacttca taatccttgg
    31861 cttaatgttt gaattttttt ggtgatagat tttttcagcc gtataaaata aatcgtaatt
    31921 ctattataga gagttgtgat tataaaaaga tagcaagaaa aaaaaagaaa gtttctatac
    31981 attttaaata ttttattcct atttctattg ctaaaaaagt agaaattcta tatttacctt
    32041 taactctcgc ttctcttgca aatgt
//
LOCUS       DARBDV010000024        30786 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000024 DARBDV010000000
VERSION     DARBDV010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.024"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     regulatory      70..167
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            283..864
                     /locus_tag="SCM56_001050"
     CDS             283..864
                     /locus_tag="SCM56_001050"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEG1109866.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            867..2174
                     /locus_tag="SCM56_001051"
     CDS             867..2174
                     /locus_tag="SCM56_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEG1109867.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            2467..2955
                     /gene="purE"
                     /locus_tag="SCM56_001052"
     CDS             2467..2955
                     /gene="purE"
                     /locus_tag="SCM56_001052"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEG1109868.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            2948..4072
                     /gene="purK"
                     /locus_tag="SCM56_001053"
     CDS             2948..4072
                     /gene="purK"
                     /locus_tag="SCM56_001053"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEG1109869.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            4085..5380
                     /gene="purB"
                     /locus_tag="SCM56_001054"
     CDS             4085..5380
                     /gene="purB"
                     /locus_tag="SCM56_001054"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEG1109870.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(5560..5937)
                     /locus_tag="SCM56_001055"
     CDS             complement(5560..5937)
                     /locus_tag="SCM56_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109871.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            6193..6921
                     /locus_tag="SCM56_001056"
     CDS             6193..6921
                     /locus_tag="SCM56_001056"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEG1109872.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            6921..7175
                     /gene="purS"
                     /locus_tag="SCM56_001057"
     CDS             6921..7175
                     /gene="purS"
                     /locus_tag="SCM56_001057"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEG1109873.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            7177..7848
                     /gene="purQ"
                     /locus_tag="SCM56_001058"
     CDS             7177..7848
                     /gene="purQ"
                     /locus_tag="SCM56_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEG1109874.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            7849..10071
                     /gene="purL"
                     /locus_tag="SCM56_001059"
     CDS             7849..10071
                     /gene="purL"
                     /locus_tag="SCM56_001059"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEG1109875.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            10056..11495
                     /locus_tag="SCM56_001060"
     CDS             10056..11495
                     /locus_tag="SCM56_001060"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEG1109876.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            11518..12570
                     /locus_tag="SCM56_001061"
     CDS             11518..12570
                     /locus_tag="SCM56_001061"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEG1109877.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            12567..13145
                     /gene="purN"
                     /locus_tag="SCM56_001062"
     CDS             12567..13145
                     /gene="purN"
                     /locus_tag="SCM56_001062"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEG1109878.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            13142..14677
                     /gene="purH"
                     /locus_tag="SCM56_001063"
     CDS             13142..14677
                     /gene="purH"
                     /locus_tag="SCM56_001063"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEG1109879.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            14693..15967
                     /gene="purD"
                     /locus_tag="SCM56_001064"
     CDS             14693..15967
                     /gene="purD"
                     /locus_tag="SCM56_001064"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEG1109880.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            16089..17003
                     /locus_tag="SCM56_001065"
     CDS             16089..17003
                     /locus_tag="SCM56_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEG1109881.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(17136..18701)
                     /locus_tag="SCM56_001066"
     CDS             complement(17136..18701)
                     /locus_tag="SCM56_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEG1109882.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(18694..20145)
                     /locus_tag="SCM56_001067"
     CDS             complement(18694..20145)
                     /locus_tag="SCM56_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEG1109883.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(20159..21175)
                     /locus_tag="SCM56_001068"
     CDS             complement(20159..21175)
                     /locus_tag="SCM56_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1109884.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            21395..22483
                     /locus_tag="SCM56_001069"
     CDS             21395..22483
                     /locus_tag="SCM56_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HEG1109885.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            22537..24063
                     /locus_tag="SCM56_001070"
     CDS             22537..24063
                     /locus_tag="SCM56_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEG1109886.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            24065..25270
                     /locus_tag="SCM56_001071"
     CDS             24065..25270
                     /locus_tag="SCM56_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1109887.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            25527..26504
                     /gene="guaC"
                     /locus_tag="SCM56_001072"
     CDS             25527..26504
                     /gene="guaC"
                     /locus_tag="SCM56_001072"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEG1109888.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            26768..28192
                     /locus_tag="SCM56_001073"
     CDS             26768..28192
                     /locus_tag="SCM56_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEG1109889.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            28653..29762
                     /gene="pdhA"
                     /locus_tag="SCM56_001074"
     CDS             28653..29762
                     /gene="pdhA"
                     /locus_tag="SCM56_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEG1109890.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            29765..30742
                     /locus_tag="SCM56_001075"
     CDS             29765..30742
                     /locus_tag="SCM56_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEG1109891.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         9894 a         5246 c         6956 g         8690 t
ORIGIN      
        1 attttttatt caaatatggc aaactgcctt cacgtgcaaa acagttgcaa gaaaatgaat
       61 ttacactatt attagtttta catacctact tatatatcgt cataatatgg atgacagttt
      121 ctagccagta ccgtaaatgc tggactataa gtaaaagatt ggctatttca cccttctttt
      181 cttatgataa agtggagggt gtttttcttt aacagaaaca tagtaggttt ttgcacaaaa
      241 gcggttattt cggtattaat aaatgataaa ggagcaaata aagtgaaaga attagttgaa
      301 cgtattcaaa aagacggacg tatattaggc gagggcgtat taaaagtcga cagcttcgtc
      361 actcaccaag tagaccctgt attgatggaa caaatgggaa aacggtttgc agaagtattt
      421 aaagaacaag gaatcacgaa agttgtgacc attgaagctt ctgggatcgc accagcttta
      481 tttgctgcaa gagaattaga tgttccaatg atcttcgcta gaaaagcaaa aagtttgacg
      541 atggatgaag aattattgac agcttctgtt tattctttta cgaaacaagt gacaagtacg
      601 atttctattt ctcgtaaatt tttatcttca gaagacaaag tattgattat cgacgatttc
      661 ttggcaaacg gacaagcggc aaaaggattg atcgaattat gccagcaagc tggagcgcaa
      721 gtagaaggaa tcgggatcgt catcgaaaag tctttccaag atggacgtca gttacttgaa
      781 gaaatgggat tgagagtcgt ttctcttgcg cgtattgctt ccttgacgaa tggacaagtt
      841 gagtttcttg aggaggacgc ataatcgtgg aaaaagaaac acaaaatgga aaagctgcgg
      901 ttttagggct gcagcatctg ctagcgatgt atgcaggagc agtagcggtg cctcttttga
      961 tcggtacagg actaggcttt aatcaagaac agatgactta tttgatttca attgatatct
     1021 ttatgtgtgg attagcgaca ttattgcaat tgacagttaa ccgatttttc ggaattggct
     1081 tgcctgtcgt tttaggatgt gcgatccaag cagtagctcc actgatcttg atcggaacca
     1141 atgaaggagt aggagctatc tacggatcta ttatcgcttc tgggatcttt gttgtgttag
     1201 tttctggtgt cttttctaaa attaaaaaac tattccctcc tatagtaaca ggaacggtta
     1261 tcaccgttat tggtttgact ttgattcctg tagcaatcga gaaaatgggt ggaggtaatg
     1321 caacagcagc tgattttggt gacaaaacaa atctgctact tgcatttgtg acgattctcc
     1381 taattgtagg cgtgcaaatg ctggcaaaag ggttcattcg ttcaatcgcg gtattgattg
     1441 gtttagtcgg aggaagcatc ttagctgctt tcatgggaat gattgatctg agcgcgattg
     1501 gagatgcgcc gttgttccat gttccacaac ccttttattt tggtaaacca actttcgacg
     1561 tatggtcgat tttactaatg atcatcattt ccatggtcag catggtagaa tcaacaggtg
     1621 tatattttgc acttggtgac attactggga aaaatgttgg agaagaagaa ttgaaaaagg
     1681 gatatcgtgc agaaggactt gccgttatct taggcggtat tttcaataca ttcccttata
     1741 ctggcttttc tcaaaatgtc ggattggttc aactttcggg aatcaaaaca agaaaaccca
     1801 tctatttctc tgcattcttc ttgattgttt taggattatt ccctaaaatc ggtgcgttag
     1861 cacaaatcat ccctgagccg gtacttggtg gaggaatgtt agtcatgttc gggatggtgg
     1921 cagtccaagg aatccgtatg ctgttggaag ttgatttcag caatgataaa aatctattga
     1981 ttgttgctgt ttctgttgga tttgggctgg gtttcaatgt tacgccgaca ttattcagtc
     2041 aaatgccgca aacattgcaa atgttcactg gaaatggcat cgtaatgagt agcattacgg
     2101 ctatcatatt gaatttgatc tttaatggat tgaaaaaaga tgaagaaata ttggaagaag
     2161 ccttcaagaa gtaatagagc aaaagaaagg ttgtgaaatc agcgttttga cctgattgtt
     2221 agagaaacaa acggaaaaat agttcctcgt attttgccgt ttgtttgagc taataccgag
     2281 ggtcagctga ttgaacaccg tttatccata aaaaggaatg tgacctaact tttgtcacat
     2341 tccttttttt catctttaat aaacacgaac aataaaatgt ttttctttgt taacattcga
     2401 aatttctttg acatcaaaaa aagtgattgt taaaatatga atgtagaaaa actgggagga
     2461 aaaacaatga cagcaaaaat ctctgtgatt atggggagta cttcagattg ggaaacaatg
     2521 aaagaagcat gtcaaatatt ggatgaattc ggtgtgtctt atgaaaaaaa ggtcgtttct
     2581 gctcatcgaa ccccagattt gatgtttgaa tatgcacaaa acgctcgaaa aaaaggaatc
     2641 aaaatcatta ttgcaggagc agggggagcc gcgcatctgc cgggtatggt cgcatcaaaa
     2701 acaactttgc cggttatcgg tgtaccggtc caatcacgaa cattaaatgg tttggattcg
     2761 cttctttcga ttgtccaaat gcctggagga gtacctgtag ctactacagc aatcggaaaa
     2821 gcgggagcaa ttaatgctgg actacttgcg atacagatgc tttccatgta tgatttagaa
     2881 ttggaggcta aattggctga aagacgttca atgttggcta aaactgttat agaaagtagt
     2941 gatcaacttg gttaaacctt tgttgcctgg ttcaacgatc gggatcgtcg gtggcggtca
     3001 attaggtcgt atgatggcga ttagtgcaaa agaaatgggc tttaaagtag gtgtgctgga
     3061 tcctgtgagc gattgtccag ccgcacaagt tgcagattgg cacattgaaa gtgtctatga
     3121 tgacaccttt gcgctgaaag agcttgccag aagaacagat gtcattacct atgaattcga
     3181 aaacgtcagt gtagaggcgt taaatgcgat tttacccatg tcatttattc ctcaagggac
     3241 agacttgctt gcgattacgc aagaccgcct gttagaaaaa tcttttttgg aaacgaataa
     3301 catcgtgatt gctccttatg caacgatcgt cagtccgacg gatatccaag atgcgattga
     3361 cgggatcggc tatccttgtg ttttgaaaac cactcgaggc ggctatgatg gcaaggggca
     3421 gtatgttttg aaaagtcggg cggatctagc tcctgccatg gaccttttaa gagaaggaac
     3481 ctgtgtttta gaagcatgga tcccgtttga aaaagagatt tcgatcatgg tggcaggaaa
     3541 tggtcaagga gatttcacga cttttccggt cgtggaaaac atccaccaca acaatattct
     3601 tcatgaaacg attgctccag ccgcgattga tcaggatgtg atcgaggagg ctgagcgtat
     3661 tgcccgtgtg attgcagaag cagtgagcct ttcaggtgtt ttaggggtag aaatgttttt
     3721 aacaaaaaca ggcgggctat atgtgaatga attagctcct cgtcctcaca attctggaca
     3781 ttattctatt gaagcttgtt ctatgagtca gttcgatgcc catattcgag gtatttgtgg
     3841 ttggccatta ggagaaaaag tacgtttgat aagcaatgca gtaatggtca atgttttggg
     3901 agaacaactg ctggaaagct atcgcttgat tgagaataag ccagactggc aattccattt
     3961 ctatggaaaa aaacaagcaa aaaaaggtcg caagatggga catatcacta tcttgacaga
     4021 agacatccag caaacgctgg aagaaatcaa acaaacgaat atttgggatt agaggagaaa
     4081 aaacatgtta gaacgttata cacgccctga aatggggaat atctggacgg aagagaacaa
     4141 ataccgtgcg tggctcgaag tcgagatttt agcagatgaa gcttgggcag aacttggtga
     4201 aatcccgcaa gaagatgtga aaaaaattcg tgagcatgca tcatttgata tcgatcggat
     4261 cttggaaatc gaaaaattaa cacgccatga tgtcgttgcg tttacacgag cggtttctga
     4321 aacattggga gaagaaagaa aatgggtcca ttatggtcta acaagtacgg atgtcgtaga
     4381 tactgcttat ggctatctca tcaagcaagc aaatgatatt ttacgggaag acttgaaacg
     4441 ttttacggaa atcattggcg aaaaagcaaa agaacacaaa tatacagtga tgatgggtcg
     4501 gacacacggt gtccatgctg aaccaactac ttttggattg aaacttgcca catggtattc
     4561 agaaatgaaa cggaacatcg aacgatttga acatgcggca aaaggagtag aagcaggaaa
     4621 aatctcagga gcagtgggaa cgttcgcaaa tatcccgcca tttattgaag agtatgtatg
     4681 taaacaattg gggatccgtc cacaagaaat ctctactcaa gttctccctc gggatctaca
     4741 tgctgaatat ttctcagcga tggcgcttat tgctacaagt attgaacgtt ttgcaacaga
     4801 gatacgcgga ttgcaaaaat ccgaaacacg tgaagtagaa gaattttttg cgaaaggtca
     4861 aaaaggttct tctgctatgc cgcacaaaag gaatccgatc ggttcagaaa atatggctgg
     4921 tctagccaga gtggtgcgcg gacacatggt cactgcctat gaaaatgtca cattatggca
     4981 tgaaagagat atttctcatt cttcagcaga acgaatcatc ttgcctgata ccacgatttt
     5041 attgaattac atgttaaatc gttttggcaa tatcgttaaa aatctaacgg tctttcctga
     5101 aaacatgaag cggaatatgt ctgcaacatt tggtttgatt tatagccagc gcgttatgct
     5161 gaaattgatc gataagggaa tgacaagaga agaagcttat gatcttgttc agccaaaaac
     5221 tgcgtacgct tgggacaacc aagtgcagtt ccgcccgctg ttagaagaag atccaaccat
     5281 cacatcggta ttatctaaag aagaaataga cgatgccttt gattatcatc atcatttgaa
     5341 aaacgttgac gtgatattca aacgagtagg tttggaataa agaagagtga gttgcaaaat
     5401 tcaattaaaa tagaatagca aaaataacca aaaaagaagc agatccgtta gcggttctgc
     5461 ttcttttagt ttattatatt ttcttatcaa taataggaac ctgattttaa aagcggctac
     5521 ctctttaccg tgcctcttta catacaaaaa atagaggaat taagcgtaaa tggcttggct
     5581 attataacta aaatgctggt tatggagatg ttgcagcaag ttttcgagaa aagcgtattc
     5641 catctcttgt ccattgaata agatctggat acgataatga tttttgaaac gcttctgttc
     5701 atgaatctga ctgattgaaa gttcgccttt gtgttctgtc tgataaagaa aagtcctttt
     5761 acggcattca gcacagcgct tggttgctcg gcggattttt cggaatgtat aaaaactagt
     5821 atctttattg cataaataaa cgtctttgat cgacagcaaa tcattatata gacaggaacg
     5881 ttcttcagct gttaattctg ataaaaaaag ctgaccttgt tttattgcat cttccatcgg
     5941 caagtgcctc ctcgaaccca ttatccaaat ttttagttaa ttctaatata ccatcattaa
     6001 gtataaatgt aaacgcttgt tgatggtata agattttcgt ttctgataaa acacgaacat
     6061 taaaagaatc taatgcctta aatgtacata tttttgttga tttataaaaa gatgattgct
     6121 aaaatttaaa gtgaaaaaac gaacaaaaaa cgatgtattt gcttttaatg taaataaaaa
     6181 ggagagacgt acatggagaa gaaagaactt ttgtatgaag gaaaagccaa aaaaatatat
     6241 cttacttctg atgagaactt gatttggata gaatatttag atcaagcaac tgcgttgaat
     6301 ggcgcgagga aagaccagat aagtggaaaa ggagaattga ataatcaaat cacaagtgca
     6361 attttccgtt atctgactca aagaggaata aaaaatcatt ttattgaaca attgtctaaa
     6421 accgaacaat tagcaaaaaa agtacaaatt atccctctgg aagtggttat ccgaaatatt
     6481 gcagcaggaa gtttctcaaa acgtctaggt gttcccgaag gtaaagaatt catcaaacca
     6541 atcattgaat tctactataa ggatgaccag ttggatgatc catttatcaa tgaagagcat
     6601 atctatttct tacaacttgc aaaaccggaa gaaatcagtg agctgaaagc ccaagcgttg
     6661 gctattaatg aaattttgac cgaattattt gctaaggtcg gtttggtttt agtagatttc
     6721 aagctagaat ttggccggtc gaatggacaa attttgcttg cggatgaagt aacaccggat
     6781 acttgccgat tatgggatga acgtacgaat gaacatatgg acaaagacgt ttaccgcaga
     6841 gatctaggtg atttgatccc agtctatcaa gaagtatata accgtctatc ggaactaact
     6901 gaagaaaagg agcgagtatg atgtatttcg taaaagtata tgtaacctat aaagattcag
     6961 tgttagatcc tcaaggggag gcagtaaaaa aagcagtcca tcgtatggga tatgaaacaa
     7021 tcgaagatat ccgaatagga aaatactttg agatacaact tgctgatgca gaagaaccaa
     7081 tcgatacagt gattgaaacg atttgtgaca agctgcttgc aaatgtaaat atggaaacat
     7141 accgatatga aatcaaaaaa atggaggaag tataacatga aatttgcggt catcgttttt
     7201 ccaggttcaa attgtgacgc agatatgtta tgggcagttc gcgagatcat gggcgctgat
     7261 gccgaatttg tacggcatga tgagtcctct ttagaaggat ttgatggggt tttactgccg
     7321 ggaggattct cctatggtga ctatttgaga tgcggtgcga ttgctcggtt ttctacaatc
     7381 atggaagaag tgattcgttt tgctgaagaa gggaaaccag tattcggtac atgcaatgga
     7441 ttccagatat tgacagaagc aggacttttg ccaggtgtac tgagacgaaa tgagtctttg
     7501 cattttattt gcaaaactgt accattgaaa gtcaacccca agacggcctt tacttcagag
     7561 tatgaagccg acgaaataat ctatcttcca gttgctcatg gggaaggaaa ttatttctgt
     7621 gacgaagaaa cacttcaaga actaaaaaaa aataatcaga ttgcctttac ttataatgac
     7681 aacatcaacg gcagcgtgga aaacattgca gggattacga acaaagcagg gaatgtgtta
     7741 ggaatgatgc ctcatccaga acgtgcagta gaagacttgc taggatcaga agatgggaaa
     7801 cggttctttg cttccatttt aaaaaacttc ggaaaggtga ccgtatagat gaaaacattg
     7861 aaagaaccga cagctgaaga gatcaagaaa aaaagaatct atgccgaatg gggattgact
     7921 gatgaagaat atcgaatgat cgaagaagat atcttaggaa gaatgcctaa ttatacggaa
     7981 actggactgt tttctgttat gtggagcgaa cattgttcct ataaaaattc taaaccggtt
     8041 ctacgcaaat ttcctactag tggacctcat gtgttgcaag gccctggaga aggcgcaggc
     8101 atcgtcgaca tcggtgatgg actagcagtt gtcttcaaag cagaaagtca caatcatcct
     8161 tcagccgtgg agccttatga aggagctgcg acaggagttg gcggaatcat cagagatatt
     8221 ttcagcatgg gggctcgtcc aattgcgttg ctagattcat tgaggttcgg cgaattagat
     8281 aatccaagaa cgaaatattt actagaagaa gtagttgcag gtatcggcgg atacggcaat
     8341 tgtatcggca taccgactgt aggcggcgaa attgcatttg atccttgtta tgaaggaaac
     8401 ccattagtca atgcaatgtg cgtaggtttg attgaacaca aacatatcca gaaagggcaa
     8461 gccgctggcg tcggtaattc gatcatgtat gtaggtgcaa aaacaggtcg cgacggtatc
     8521 cacggagcaa cttttgcctc agaagaattt aatcaagaag aagaacagca acgttctgct
     8581 gtacaagtag gcgatccttt tatggaaaag ctgctgcttg aagcgtgttt ggaattaatc
     8641 ttgcatcatt cggattctct aataggtata caggatatgg gagcagcagg acttgtttca
     8701 tctagtgcgg aaatggcttc aaaagctggc tcaggactta tattgacatt agatgaagtc
     8761 ccacaaagag aaactgggat gacaccttat gaaatgatgt tatcggaatc acaagaacgg
     8821 atgttgattt gtgttaaatc aggagaggaa gaaaaaatcc aagaactatt tcagaagtat
     8881 gatctagatg cagtcactat cggtaaagta acagacgacg ggcaatatcg gctatatcat
     8941 caaggagaag aagtagcaaa tctgccagtc gatgcattag ctgaagatgc accagtttat
     9001 cataaagaaa tgaaagaacc agctcgaatc gtagaatttc agcagttagc tccttatcag
     9061 ccagttatcg aagaacctgg acaagtattg cttgatctgc tgcagcaacc gacgattgct
     9121 tcaaaacgtt caatctacga aacttatgat tcacaagtac aaaccaatac agttgtccgt
     9181 ccaggaagcg atgctgctgt gatgagggtt agagggacga ataaagcgtt agctatgacc
     9241 acagactgca acgcgcgcta tctgtatttg gatccaaaaa tcggaggaca aatagcagtg
     9301 gcagaagctg ctagaaatat tattgctagc ggtggtaagc ccttggcgat caccgactgc
     9361 ctgaactatg gttctcctga taaaccagaa gttttttggg aattatcgac ttcagctgac
     9421 ggaattgctg ctgcgtgtga aacgttagga actcctgtta tttcaggaaa tgtctcttta
     9481 tataatgaaa cggatggtca agcaatctat ccaacaccaa tgattggtat ggtcggattg
     9541 attgaggatc ataaacatat cactactcag gaattcaaaa aatcagggga tctaatctat
     9601 atattaggta agacgtttgc agattttgat ggcagtgaat tacaaaagat gcagcttgga
     9661 agaatcgaag gtgtgatccg aaactttgat ctatccattg aaaagagaaa ccaagagtta
     9721 gtattgacag ccatccaaaa cggattgatt gaaagtgctc atgactgttc agaaggtggc
     9781 ttggcaattg cactggcaga atcagctttc aaacatcagc tagggatatc tgtacagttt
     9841 gaattgtcct ctgctcaatt atttgcagaa actcaatcaa gatttgtgtt gacagtcgca
     9901 ccggaaaata agacacgatt cgaagaaatg atgggagatg ctgctgtttt agcaggtaag
     9961 gtgacggatg aagcgataat cgaaatttca gcaactgacg gtcagatcaa aatagaaact
    10021 gcagtagcaa gaaagtgctg ggaggatgca atcgtatgtc ttatgaagtg aagagtttaa
    10081 atgaagaatg cgggatcttc ggtgtttggg gtcatccaga cgccgctagt gtggcatact
    10141 tcgggctcca tagtcttcag catcgaggac aagaaggtgc aggtatcgtg tccaatgatc
    10201 gaggaaagct taaggggcat cgagatttgg ggctgctttc tgaggtattc aaagatcaaa
    10261 gaaaattgca gcagttaaca ggagaagctg caatcggaca tgtacgttat gcaacagcag
    10321 gcaatggaag tgtggacaat attcagcctt ttctattcaa atttcatgat cagtcgatcg
    10381 gcttagcgca taatggtaac ttgatcaatg ctcgttccct tagaaaagag ttagaggaaa
    10441 atggggcgat cttccattct aactccgata cggagatatt gatgcatttg atccgacgaa
    10501 gtgaagaaac agatttttta aataaattaa aaagtgcttt gaatgaagtc aaaggtggct
    10561 ttgcctattt attaatgaca gaaacggcaa tgattgccgc tttggaccca aatggttttc
    10621 gtccattagc aatcggacaa atgaaaaacg gagcatacgt gattgcctcg gaaacttgtg
    10681 cattagaagt tattggggca cgttttataa gagatgtcca gcctggtgag atagtgatca
    10741 tcaatgacga aggaatccag attgattgct ttacgaagga cacgcagctt tctatttgtt
    10801 cgatggaata tatctatttt gccagaccag attcaaacat cgcagggatc aatgtgcata
    10861 ctgcacggaa aaatatggga cggaatttag cgaaagaatc gccagtaaaa gcagatatgg
    10921 tcgttggcgt acctaattct tctttgtcag ccgcaagcgg ttatgcggaa gctagtggaa
    10981 tcccttatga aatcggatta gtaaaaaatc aatacattgc ccgaacattt atccagccaa
    11041 cgcctgagct aagagagcaa ggtgttcgaa tgaaattatc tgctgtacga ggagtagtgg
    11101 aaggtaaaaa ggtgattatg gtcgatgatt caattgttcg gggtacgaca agcagacgga
    11161 tcgtccatct tttgaaagaa gcaggggcaa aagaagtcca tgtgaggatt gcttctcctc
    11221 ctttgaaata tccatgtttt tacgggattg atattcaaac gcgtaaagaa ctcattgcgg
    11281 caaatcacac gattgatgaa atcaaagaat gtatcggtgc agattcttta ggttttttaa
    11341 gtgaagaggg tctgatcgaa tcgatcggtc tggaaaaaga tgctccgtat tcaggattgt
    11401 gtatggctta tttcaacgga gattatccga cgccgttata tgactatgaa gaaaaatatc
    11461 aggaatcact aaaagaaaaa gtatcgttct tttaaaagca gaccaagaag ggagtatatg
    11521 aattcaatgg caaatgcata cgcaaaagct ggcgtagatg tcgaagctgg atatgaagta
    11581 gtagaacgga tcaagaagca tgtcaaaaaa acggaacgtt taggtatgat gggcgctata
    11641 ggaggattcg gcggttgttt tgatttatcg gtctttcaat taaaagagcc agtccttgtc
    11701 tcggggaccg atggcgtggg gacgaaatta gcagtggcaa tcgaagcaga caaacacgat
    11761 acaatcggga tcgactgcgt agcgatgtgt gtcaatgata tcttagcaca aggcgcagag
    11821 ccgttatatt tcttagacta cttagcaacg gggaaaaatg atccgttacg tttggaaaat
    11881 gtagtaaaag gtgtagcaga tggatgtgtt caaggaaata tggcgctgat tggcggagaa
    11941 acagctgaaa tgcctggtat gtactcaggt aatgattatg atctagccgg atttgctgta
    12001 gggatagctg aaaaatctca attattgaca ggagaaacga tcaacccagg agatgtgttg
    12061 cttggaatag catctagcgg gattcattca aatgggtact ctcttgttcg caaaattttc
    12121 tttgagcagc agcagttttc tttatcatca aaactgccag aattgaaaaa aacacttggc
    12181 gaagaattgc ttgagccaac gaaaatctat gtttcttcct tgcttccgct gatcaaagaa
    12241 aagaaaataa aaggagctgc acatatcact ggtggagggt ttgtagaaaa tatcccgcga
    12301 atgttaccag aatctatggc tgcacaaatc aaacttggct catggccgat tcttccaata
    12361 ttcggctgtt tacaaaaata tggtgaaatt cctgagatgg agatgtatga aatcttcaat
    12421 atgggcatcg gtatggtcct ggcagtcgaa gaatcgaaag taaaagaaat agtacacgaa
    12481 attgagcaag ctggcgaaaa agtttatgaa atcgggaaag tgatcgccaa agaaaaacat
    12541 cgtgtggtgt ttataggaga agaacgatga gaatcgctgt ttttgcttct ggaaacggaa
    12601 gcaactttca agcgctagcc gattatctat cgaaaaaagg attggaatct tcgattgact
    12661 ggttgttttg tgatcagcct gaagcatatg ttttgaaacg tgcaactgcg ttgagtgtac
    12721 cggcagattg tttttcacct aaagaatttg attcaaaaaa agagtatgaa gaagcaattt
    12781 tgcataaatt gaaagaaaaa aagatcgatt tgatcgtatt agctggatat atgcgaatca
    12841 ttggaccggt attgctgaaa aattatgata aaagaatcat caatatccat ccgtctcttt
    12901 tacctgcctt ccctggtttg catggcatac gtgatgcctt tgaagctgga gtaaaagaaa
    12961 cgggtgtcac aattcattat atcgaccaag gtgtagatac tggaccaatc atccggcagg
    13021 aaaaagtgag aattgaacag gaggacacgt tcgattcttt agaagaaaaa atccatagag
    13081 tagagcatcg aatctatcca gaagtgatta gtgaaattat tgaaaatgga ggctatctga
    13141 aatgacaaga gcattgatca gtgtatcaga taaaactggg attgtagcat tcgcccaagg
    13201 tttgattgaa caaggagtag aaatcatttc aacaggagga acaaagcatg cgttggataa
    13261 agctgggatc aaaacgcttt cgatcgaaga agtgacaggg tttcctgaaa tgatggatgg
    13321 ccgtgtcaaa acacttcatc ctaaaatcca tggtggtctt ttaggaagac gcgatctccc
    13381 tagccatatg gaggcaatgg aaacggaaaa tatccaacca atcgactttg tttgtgtcaa
    13441 tttatatcct ttcaaagaaa cgattatgaa accagatgtc gaaatagcag aagctattga
    13501 aaacattgat atcggtggtc ctagtatgct ccgtagtgca gcaaaaaacc atgaatttgt
    13561 cacctctgtt gttgatccat ctgactatga agaagtactt tcagaattac gtcagtatgg
    13621 gcatacttca ctttctttca gacaaaaact agcggcaaaa gtctttcgtc atactgcggc
    13681 atatgatgca ctgatcgctc aatacttaac ggattgggtg gaagaaaaag aaccggaaaa
    13741 attgacactt acgtatgaac gaaaacaaac gcttcgttac ggtgaaaata gccatcaagc
    13801 cgctgccttt tatcagtccg ctcttctgga aaaatattcg atcgcagcag caaaacaact
    13861 ccatgggaaa gaactttctt ataataatat ccgagatgca gatgctgcgc tacggatcat
    13921 gaaggaatat gacgagccaa cggtagtcgc cttgaaacac atgaatcctt gtggtattgg
    13981 gacagcatct acgattttag aagcgtggga agcagcgtat gaagcagatc ctgtttcaat
    14041 ctttggtggg atcatcgttt tgaatcgaga agtagatctg ccaacagcag aacagatgca
    14101 taagctgttt ctagaaatca tcattgcccc ttcttataca caagaagctt tggaattact
    14161 gcaaaaaaag aaaaacatcc gattactgac agtagacttt gcgcaggaag aaaatcaaaa
    14221 tcgagaagaa acggtctcag tcttaggtgg acttcttgta caagatcaag atctggcaat
    14281 agaaaatgaa gaagagtgga aagtcgtgac aaaacgtaag ccgactccag cagaagcaaa
    14341 agcattagcg tttgcttgga gagctgtcaa acatgtgaaa tctaatgcga tcgtactggc
    14401 aaacgaacgt cagacagtag gaatcggagc cgggcagatg aatcgagtcg gttcagtaaa
    14461 aattgcgatt gaacaagcgc aagagaaaat ggaaggtgca gttttagcta gcgatgctta
    14521 ttttccaatg gatgattcgg ttgaatatgc agccaaacat ggtataaaag caatcattca
    14581 gccaggagga agcatcaaag accaagattc tattgatatg gcgaataaat acgatctagc
    14641 aatggtcttt actgatgttc ggcattttag acattagaga ggagtcggat aaatgaacat
    14701 tttggtgatc ggtgcaggtg gtcgtgaaca tgcaataagt aaaaaacttt tagccagtcc
    14761 gaaggtggat acggtgtatt gtgcgccagg aaatccgggg atgacaaaag atggtatcaa
    14821 ttgtgtcgct atcagtgagc gagatcattc agcgttaatc cattttgtta agaaagaggc
    14881 tattgattgg agtatcgttg gtcctgaagt tcctttgtta aatgggatcg tcgatgattt
    14941 tatggcggaa ggattgaaaa tcttcggtcc gacaaaagca gcagctctga tcgaaggttc
    15001 gaaaagtttt gctaaacaat taatggatac ttatcaaatc ccgacagcta aatccagaag
    15061 ttttgaatgt tacgaaaaag cagcagcgta tatcaaagaa aatggtgcgc caatcgtagt
    15121 gaaagcggac ggcttagcag ctgggaaagg tgtagtcgtg gctaccacag tgaaggaagc
    15181 gctggatgcg gcagaacaaa tgttgaacca gcatcgattt ggggatagta gcaaaaaaat
    15241 cgtcgtagaa gagtttttgg caggagaaga attctccctt ttggcgtttg tccgtaatac
    15301 caaggtgtat ccgatggtca tttcgcaaga tcataagcgg gcatttgata acgataaagg
    15361 gccaaataca ggcggaatgg gtgcttattc accagtaccg caaatttcaa aaaagatgat
    15421 tgaaaaagct gtggaagaag ttttaaaacc agcggctttg ggaatgaaag aactaggacg
    15481 ctctttcacg ggtgttttat atgcgggttt gattgctacg aaggaaggac caaaagtgat
    15541 cgaattcaat gcgcgtttcg gtgatcctga aacgcaagtc gttctttctc ggttgaagag
    15601 tgactttgcg caagtaatcg atgatttatt agagaatcgt acagtagagt taaagtggca
    15661 gcaagaagga tacaatgtag gcgttgtggt tgcagctgcc ggttaccccg aagaatatga
    15721 gacaggaatg gttctaccag attttctgga agaagagctt tctgtttact atgctggagt
    15781 ttcctcaaat aacggcaaac tagttggagc aggaggacgt ctttatttag tagaagcata
    15841 cggtgagacg atcaaagaag cacagaaaaa aatttacacc gcgttaatgg ataaaacgac
    15901 agatggaaca ttttatcgta cagatattgg agaaaaagct gccaatagcg aactgatcgt
    15961 gaagtaaatc agctgcttcc atttataaaa aatacaacaa acataatcgg aagcgccgat
    16021 tttagtgaag ccttttcata aagagtgtgg tacacttttg ttgtgtatat aaataaggag
    16081 gagttcagat gaaaaaaatt caattaggta cttctgatca gaaagcagct gcagtgatct
    16141 taggctgtat gcgtatcaac aatgcggaag atccagtaaa agtgattgag acagcttatg
    16201 aaaacggcat tgattttttt gaccatgcag atatttatgg aaacggtgaa tgtgaagaaa
    16261 tctttgcaga tgctctagca aaaacatcaa tcaaacgaga agatcttttt attcaaacaa
    16321 aatgtggaat cgttccaggt gtgatgtttg atttttctaa aaaacatatc attgaatctg
    16381 tagaaaaaag tttgacccgt ctgaaaatgg attatgtgga tgctttactt cttcatcgtc
    16441 cagatacatt ggtagaacca gaggaagtag ctgaagcgtt tgatgaattg gaacggtcag
    16501 gaaaagttcg ttattttggc gtcagcaatc aaaaaccaat gcaaatcgaa ttgctgaaaa
    16561 aatatgttcg tcaaccatta gtagctaatc agctgcaatt tggcttgaaa catactggaa
    16621 tgatcgatca agggattcat gtcaatatga cagatgaagc aagtatcgat catgacggta
    16681 gtgtacttga ttattcccga ttgcatgata tgacgatcca agcatggtca ccataccagt
    16741 atggattttt tgaaggtgta tttatcggga acgaaaaatt ccctgaatta aaccaaacat
    16801 tagaagaact agcagctaaa tatcaaacga caccaactgg cttggcttct gcttggattt
    16861 tacgccatcc agccaatatg caagtgatcg ccggtacgat gaacgtaggg cgaatcaagg
    16921 aaatcgctca agcttcagaa attaatatgt ctcgggaaga ttggtacgaa ttgtaccgtg
    16981 ctgctggaaa cattttacct taaagattgt cataagcagg tcaaaagggc tataaattaa
    17041 aaagacagaa taacttgaaa gctgttttca gcttcgagat attctgtctt tttttattgc
    17101 aaatggaaag agtatcattg tacggatttc gtttattagt tatttttgtt tttaaggaaa
    17161 agctgtgtcc gatattcttt tggagaaatc ccttgttttt tcttaaataa ataactgaaa
    17221 tagttagggt cattataacc aatttcaaaa gcaatatctg aaatacgata gtctgattgc
    17281 tgcaataatt tttttgctaa ttcgattctt tgttctgtca gataatcgat gaatgtttgg
    17341 ttaaacgatt gagaaaaaat cgtactgaaa tgagagggac ttaatgcaac ttgttgagcg
    17401 acccaagtta aggacatatc tggatcggta aaattttctt tgataaaatc caatgcatgg
    17461 ttcaaaattg gctgatattt gttccttgaa ggctgtgagg gcagagcagt taagaaagaa
    17521 atgaacatat aaactgtcga acggtaaagc gagtcgtcgg cgctgattgc taagcgttga
    17581 ctggtggatg tgcaatgaac catttcttgc gggattttct tttctttcag gcagacatgc
    17641 tggagtagct tagtcaagac aaataaacga tataatcgtg ttcgagttgg tgtgccttgt
    17701 ggtttcgtca agtgttcgat gtacttagat acttcgtttt tcgataatga agacaaatca
    17761 agtgagaggt caaagtaaaa gacagaatca actggaagtt tcgcttcttg aatcaaatcc
    17821 tcatagctga taatttgttc tttaagggaa ctggcggttt caagtatcat ctgcgtgatc
    17881 tcaaaagatg caggtaattc gctgattctt tctgcgacag gaccaatggc gacagaaaaa
    17941 ttattttttt gtcccttttc taattcatgg accaacgttt gggctacctg ataactggtc
    18001 tcgatcacat tttcttcaag agaatcaact attaaaaatt tgatgaatcg agaggaaaca
    18061 gcagagaaga tcacttgttg attctcccta aataaaaact tcaaataatc actgagatga
    18121 gcgtaatctt caaagtctgt actgacttgg tttgctgcta ataatacggt catttttttc
    18181 cctgctaaag agcgattgaa agattcagca agtttcaatg tttcactgat cgtaagtgag
    18241 cctttgaaca aaccgttcaa gaaatgattt tttttcaatt cgaaaacaag atcagaagaa
    18301 gtgtgcgttt ctttttgctg caaatctaaa gcagttactg ctttttctat tgcttgtttc
    18361 aattcgctac ttttaatcgg tttaagcaga taatcatctg cctgtacctg gattgctcct
    18421 ttcatataat cgaaatcgtc gaatccacta ataaatatga gacgagtcca aggaagtagt
    18481 ttccttgctt cttttgaaaa agtcaacccg tccatgaaag gcatttggat atcagtcaga
    18541 atgatgtcgg gctgtaagtc gataattgaa gacagtgcca tctcaccatc tccagcttcg
    18601 ccgacataag tgattggata ataagaacca aggacagtta tttgttcccg caagttattg
    18661 cgaatgaggt attcgtcttc gatgataaaa actttataca tgtttgtttc ctccaattgt
    18721 tttgggagat agagggatca tcagtgtgac agttgttcct tctcggtatt tgctgtcaat
    18781 cgtgataggt ttttgatcat ttccgtaata aagaaggaga cgcttgttta cgttgtaaag
    18841 tccgtagccg atactgaaat ctgaatcgat ccctttggct aactctttac gtatttctgc
    18901 cagtttttct gcagggatgc caatcccgtt gtctataact ttgaattgga tcatctcatc
    18961 aattttgcat gcggtaatac gaataaatcc tttgtgacgg acgtacttcg ttccatggta
    19021 tacagcattt tcaatcaatg gctgcaagat catttttaaa atagaaacag gaaaaagtgt
    19081 ttctggtata tcgatcttat agtcaagcat atggccgtat cttattttta agatgactag
    19141 ataatcattg atatggctaa tttctttttc aagaggaatc cagtcttgtc ctttgcttaa
    19201 agacgtacga aaaaagccgg aaagtgcatg ggtcatagtc agagcttctt caaagcagtc
    19261 ttgttcgatc aaagaaataa tggcatccaa actgttataa ataaaatggg gagtaatctg
    19321 agcttgcaat acacgaactt cactttgtgc taaatggtat tgtttcaaag cgttctcccg
    19381 taaagactgg tttagatctt ccgccatttt attcatgcta gcggtaagtg tttgtaattc
    19441 cagtgtttcc ggcagggtta aacgaaagcc aaggttccct gaagaaagtt gttctgacat
    19501 ctcgatcagc tgattgatcg gttcttggac ctttctctct aaaaagcgac gattcttttt
    19561 cgcatagaaa aaaatggtac taagaactcc aagttccact attgttaata gaatgagcga
    19621 gcggacaagt gtcgtatttt ttttgcttgc taagtcgatc tccacacgaa caaaatcttg
    19681 taagatatct gacagcagtt gagtgacaga atctacctga ttgatgatct cttcattggt
    19741 atccataaaa tcttttgcca agatattatt gatcagctcg ttttgataat cttccagtgt
    19801 atcgatcgta cgtagcgata catctaacgt actaatttct ttaagtgtat ttgtattatt
    19861 tttgatctct cgaatatcat tcctaagttc ttctaggata ttttcctcat tgaactcatc
    19921 tacggttact tggccagaaa tcaagtccca catactttcc aaaacttttt gatcaacctt
    19981 caaagcaatt gcatctgctt tttggatatt tgcgatcgtc tgttgataaa tcaaaagctg
    20041 ttgcgaatat aacagtatgc tcagtagcaa agggataaca gcaaatagaa gcatggcttt
    20101 gtttgcttgg gcaaaaatct gttttatgct taaatttttt gtcatgatac ttctccagtc
    20161 aataatttct ggttgggatg ttagctatat tctgctggga aaagacagtc tcggaaataa
    20221 aaatatcttt aggtaatgtt tttcctgttt gaatatttcc aaaataacga gtaacggtat
    20281 tggctacata ccagccgaca tcaggtttac actcgactac acaattgacg attcctgagc
    20341 gtagcttctc gatcatatct tcttgtccat caatagttac aatgatcaaa tcttctccag
    20401 gtttgattga tgtttctttt atcgcagcta aagcaccgtg agtcatttca tcactatggc
    20461 tgtataatac atcgatttcg gataaatcat ttgtttctaa atatcgtttc atctggtctt
    20521 ttcctttcat ccgaataaaa tcaccgacaa ttcggtgaac aatagtcatc ggaggtttat
    20581 ggtcgatggt tccatctttt tttagggttg gtttacgatt gattgattcc aaaaatcctt
    20641 cggagcgtag cttggtcggt gtggagttct ctgaaccagc aagttctagg attctgattt
    20701 cctgtttctt tgaatttgtg aagtgattgc tgacaaataa acccgcccga tttccttctg
    20761 ctttgaaact ggggccaatg tgggtgacga aaagctcggg atcattcgtt tgaatatgtc
    20821 tgtcgacaag tatgacaggg atccctgctc gctttgcttc ttttaataca gagtcccacc
    20881 cggattcttg caaaggagta aaaatgatca tatccacttt ataggcaatg aacgtccgta
    20941 tatcttggat ttgccgttcc tgattcatgt agccatttcg gtagagaact tggtaatctt
    21001 ctttttcaag cgcagtgata attgattcag tatgcttttt ccgccagcta ctctccgttc
    21061 ctgattgcga aaaaccaacg actagtgggt caggtgcttc ctttgtatta ggtgtacacc
    21121 ccatcatttg gtaagcgatt acaacaaggg ctaaaaaaaa taaaacacgt ttcactttaa
    21181 atcactccat ctggatttgt tagtactatc ttatcagata aaagggaaaa tcaaagtcaa
    21241 aaggaatcaa tcgaaaaaaa tccaagaaac ttaaaaaaat ccaagacaaa aaaagtacac
    21301 ctaaaaaatt taaagtaaat cccataaaaa aatcaatgtt aacgctttct ttattagcta
    21361 tactcggtgt atcaattgaa atgggggata aaaaatgaaa aacaaattta gaaagattgt
    21421 tgggactatc gctattttga ccgcaagcat tctattaagt gcgtgtggca attcaggatc
    21481 tgcagatgat agtacggggt atgtaggaat cgctatgccg accaaatcag cagaaagatg
    21541 gatcgctgat gggaacaaca tggtctcgga attagaaaaa ctgggttata aaacagatct
    21601 tcaatatgga gaagataaag tagaaaatca agtagcacaa atagaaaata tgattacaaa
    21661 aggggtagat acacttgtga ttgcttctat cgatggttcg gctttaactg atgtgttagc
    21721 taaagcaaaa gaagcagata tcaaggtgat cgcctatgat cgtttactga tgaattcaga
    21781 aaatgtcgat tattacgcga cttttgataa ttttggagta ggtgtatcgc aagcagctta
    21841 tattgaagag cacttaggat tgaaagaagg aaaaggacca ttcacgattg aattatttgg
    21901 tggctcgcct gacgacaaca acgcactaat caattacaac ggagtgatgt ctgttttaca
    21961 accatatatg gacaacggtc agttggtcgt gccatctgga cagacgagtt tcagtcaaat
    22021 cgctacgctt cgctgggatg gttctacggc gcaagcaagg atggataatc tgttaagcgc
    22081 aaattatact gaccaaacat tggatgctgt tttgtcgcca tatgatccaa taagtttagg
    22141 aatcatttcg tcattaaaag gcgtggggta tgggagtgaa agtaaacctt tacctgtgat
    22201 tactggacaa gatgcgacag ttgctggcgt taaatcgatc attgccgggg aacaaacaca
    22261 aacgatcttt aaagacacac gtatcttagc aaaaaatacg attgaaatga tcaaagcaat
    22321 cagtgatgga gaagaggttc cggtcaatga tactgaaact tatgataatg gggtgaaaac
    22381 tgtccctacg tatttagcaa atacagtatc tgtcgataaa gataattatc aagcagagct
    22441 gatcgacacc gattattata aagaatccga tctgaaaaat taagaggaac caaacaagga
    22501 actaaagaga tctaagtcag agagaggata agaattatgg cggattgtat tttaaaaatg
    22561 gcagatatca ccaaaaaatt ttctggaatc cgagcgttag attgcgtgaa tctgatgatt
    22621 gaacgtgggg aaatccatgc attgtgcgga gaaaacggag cgggaaaatc aacattgatg
    22681 aatgtcttga gcggcttgta tccatatggc tcctatgacg gggaaatctt ttatgatgga
    22741 aagctgtgtc aatttaaaga tttgaaagat agtgaagaaa aaggcatcgt catcattcat
    22801 caagaattag cactaagtcc ttatctttca ataaaagaga atatttttct cggaaatgaa
    22861 caagcagaaa gaggcgtgat tgattgggaa ctgacagaaa agaaaacata tgaactgctg
    22921 aataaagtcg gtttgtctat cgacccgaat cttctagtat cccagattgg cgtagggcaa
    22981 cagcaactag ttgaaatagc taaagcatta tcaaaatccg tccgtctgtt gattttggat
    23041 gagcctaccg ctgcattgaa tgaagaagaa agcgcgaatt tgctgagatt gatccgagag
    23101 ttgaaagagc aaggtgtaac ctccatcatt atttcacata agctgaatga aatcgtggat
    23161 atcgcagatc gaatcaccat tttacgggat ggacagacga ttgagacact agaaaatgaa
    23221 atgatcagcg aagagcatat tattcgcggg atggtgggca gagatttagt tgatcgttat
    23281 cctgaaagaa aaccggatat tggagaagtt tattttaaag tagaagactg gacggtctat
    23341 caccctcttg atcacgagcg aatcataaac gaccacatca acttgcattt gaaaaaagga
    23401 gagattgtag gaatagctgg actgatggga gcgggcagaa ctgaattcgc tatgagtcta
    23461 tttggacatt cttatggaag caagatatcc ggacgtatat taaaggaagg ggcagagata
    23521 tcagttaaga ccgttccttc agccattgca aacggactcg cttatgtctc ggaggaccga
    23581 aaatctttag gactgaatct gctgatggat attcgggaaa acgtgacgct gagcagtttg
    23641 gataaaataa gtcaaaaagg agtattagat aaagaaaaag aagtaactga ggcagaaaat
    23701 ttccggaaaa agatgcgtat aaaaacaaac tccatccatc aaattgtcag tagtcttagt
    23761 ggtgggaatc aacaaaaagt cgttttatcc aagtggctga tgacacaacc cgatattctt
    23821 tttttggatg agccaacccg tgggatagat gttggggcaa aatatgagat ctacacgatt
    23881 atcgaagaaa tggcagcaaa tggcaaagct gtctgtctta tctcttctga acttcctgaa
    23941 atattaggga tgtgtgatcg aatctatacg atgaacgaag gacgtattac aggagaagtc
    24001 tcgcgagaag atgcagatca ggaagtcttg atgaaattaa tgacgaaaga agaggtgggt
    24061 taagttggaa caggcaaaaa atcttacgga taaaaaagag aaaaaactaa atttgaaaag
    24121 tagagcagct gatctttttg gtcgctacag catggtgatc attttagcgg gactacttat
    24181 tgcgtttcaa ttgatgacgg atggtatttt ctggcgtcca ttgaatataa ctaaccttgt
    24241 tctgcaaaat agccatatat tagtactagc tgcagggatg ttattagttg tgctcttagg
    24301 tcatgtggat ttgtcggtag ggtcagtgat ggcgtttgtt ggtgcaattg cgggcatgat
    24361 gatggtcaat cgccaaatga acccgtggat gacagttatc gcatgtcttg cagtaggcgg
    24421 gctgatcggc gcatggcaag gtttttgggt agcttatgca gggataccgg cgtttatcgt
    24481 gactttagca ggattgctga tgtttcgtgg actgacacaa gttgttctag gaggccaatc
    24541 tctagcacct tttccagctg ttttccaaaa aatgtctacg ggttatttac cagacttaag
    24601 cgaaggaagc agccttcact tcttgacatt gattttagga atgatcatca cgatcgtgtt
    24661 agttgttggt caatggcgaa caagaaatcg tcggaaaaat catttatttg aagtggaatc
    24721 tccaacagct ttcttggtaa aggcggtatt tacagcagtc gttgtactag gaataactta
    24781 tatctttgcc tcttatcaag ggtatccgat tattttgatc attcttggtt tgatcgttgc
    24841 agcatatggg tttttgacaa ataaaacagt agcaggtcga cagatttatg cgacgggcgg
    24901 aaatcgaaaa gcggctgaac tatcagggat caaaacaaag cgaatcacgt tctgggtatt
    24961 cgtcaacatg ggagtcatgg cagctttagc tggattagtt ttagctgctc gattgaatgc
    25021 ggcaacacct caagcaggta catcgatgga actggatgca atggcggcag tatattttgg
    25081 tggtgcttcg acttccggcg gcattggaac aatcatgggt gcgattgtcg gtggcttggt
    25141 catgggtgta ttgaacaatg gcatgtcgat tttaggcgtc ggggtagact ggcagcaggc
    25201 aatcaaagga ttgatcttgc ttttagccgt tgtgctagac ttgtataata agaaaaaacg
    25261 aacaacgtag gagaacgccg gttattcaga aaaaagactg tgacaagaca tttgtcacag
    25321 tctttttttc gctaaaagcg aacattcatt gtttcgtttg tatagactaa aaataatata
    25381 tgttttttta ccattcgtat aaattctctt tttgctcttg atttttttgc ttaattgagt
    25441 taaattacat agggcgctaa aaaaagcctt gattaaatta ataatgttcg tgttttgctg
    25501 gatgtgaaca tcaggaggat aatgtaatga aagtatttga ttacgaagat attcaattaa
    25561 ttcctaataa atgtatcgta aatagccgtt ctgaatgtga tacaacggtc acattaggta
    25621 aacatacatt caaaatgcca gtagtgcctg ccaacatgca gacgattatt gatgaaacaa
    25681 tcgcagaatt cttagcggaa aatggttatt tctatatcat gcatcggttt gatgaagcag
    25741 ctcgaattcc gtttatcaaa aaaatgaaaa agcgtggatt gattacctcg atcagtgttg
    25801 gcgtcaagaa agaggaatat tcctttatag agaagttggc agaagaatcg ctaaatcctg
    25861 attatatcac tatcgacatc gcgcatggac atgctaactc agttattgat atgattcagc
    25921 atattaaaaa atatttgcct gaaacttttg tgattgccgg taatgttggt acaccggaag
    25981 ccgtccgtga attggaaaat gcgggcgcag atgcaacaaa agtcgggatt ggtccaggaa
    26041 aagtatgtat tacaaaaatc aagacaggct ttgggactgg aggctggcaa ctggcagctc
    26101 ttagatggtg tgcgaaagca gctcgcaagc cgatcatcgc tgacggtggg atcagaacac
    26161 atggcgatat tgcaaaatct gtccgttttg gtgcgacaat ggtcatgatt ggttcattgt
    26221 ttgccggtca cgaggaatct cctggcgaaa caaaagtcga aaatggaatc gtgtataaag
    26281 aatactttgg ttctgcttca gaattccaaa aaggcgaaaa acgaaatgtt gaaggaaaga
    26341 aaatctggat ccaacacaaa ggatctttga aagatacact agtggaaatg caacaagacc
    26401 ttcaatcatc tatttcttat gctggaggaa gagatctgga agccattcga aaagttgact
    26461 atgtcatcgt gaaaaattcg atttttaatg gagacacaat ctaaaaaata aataaaatat
    26521 taggatgttg tgaaaaagtt gtcctgcatt aagtaataag aacagccaaa caaaaatagt
    26581 tctttatatg ttttgtttgg ctggaattat tatcgcagat acaagctttt gaacatcatt
    26641 attcagaata agggactgtg ataagatttt tgtcacagtc ccttatgttt tgcaaaaaaa
    26701 gtttttcttt tttctcgtaa attcggtaaa atcaaaagaa aaaatagaca atagaaaaga
    26761 gaagcttatg tgggaaaaag tagagaaaaa acaaggaccg atttatcgtc aaattatgga
    26821 acagattatg agagaaatcg aaaacggaag gatcaatcct ggaagtcaac ttgaatcaga
    26881 acgaaaacta gggcaacttt ttggtgtgaa tcgttccacc gttgtccatg cattggaaga
    26941 gcttagggag cttggggtac taacgagcaa acgtggcagc ggatggtttg ttaatcagac
    27001 tgaatggggg aaattcgctg ttccaagaat cgattggcgg caaatgatct ctcctcgtta
    27061 tgagcagacg gactggtatg agcaaaaaat aagagaagca aagcagatcc aaaaaacatc
    27121 gtttcttgat ttatacgcta gcgagatgcc agatgagctt ttgccgaatt ttgagtttcc
    27181 ttcttattct cttgaagaaa tcttagcgga agaaaaagaa atggatcttt taggctatac
    27241 tccgttacgt aacaaaatca aacggtattt ggaaagtaaa tttgaattgg cattttcccc
    27301 caaccagctt cttgtgactg gtggcggtca gcaagcaatc tttttgattt tacaaacgat
    27361 tctttcagct ggagagggga tagctgtaga gtcaccttct tttttttacc gattacctct
    27421 tttcaaagct tcaggaacgc gtttatttgg gataccgatg gatgaagaag ggatcgactt
    27481 agaaaaactg aaagaatcga tccataaaaa taaattgaaa gcagtgctta tcaacccgaa
    27541 ttttcaaaac cctacaggaa aagtgatgag tcaaaaacga cgagaaaagc tagtagaatt
    27601 gtgtcgacag tatcggcttc caattattga agacgatgta tttacggatc tttcatttct
    27661 aaaccaagaa gaagtccttc ctatacgaac gattgatccg gataatgtgt tatacgtcgg
    27721 ttcgttatct agggttttag gaaaaacaac gaaaatcggt tgggtgatag gacctgagac
    27781 ttttatctcg caactagctc aagcgcaaga aatgatggag ttttcgatga atatccttac
    27841 acaaatcaca gtggctaatg tatttaatga agcaattgat ttgaaaatgc aagatgtaag
    27901 aaaaaagttg aaagaaaaaa gccgactgat aagcgcttgg ggagattcac aatcgttttt
    27961 caagcttcat tcaattttag gtggttacta tgcctggatt acttgggatg ggaaaaaact
    28021 agaccccgaa ctagcagaaa aagtagtaga tatgggtctt ggaattgcac caagtctact
    28081 ttttgggcaa gatataaatg gaatcagaat caattacagt aggctggatg aaaaccaaat
    28141 accgttgttt tattcaaaga tgaaaattct agaaaagtgg ttaacagaat aatttcacaa
    28201 agtaaataga ttaaagaata taacagcttg gatttaaatt tcaagctgtt tctttttttt
    28261 cggaaaaaga atacgatata aagaaactaa gaaaaaacca ttatttgtac gtccgttcac
    28321 gatgtgtaaa taaaataaat gtttgcaaca aaactgtata acaccaacgt ttttaaatag
    28381 aaacagatgg ttgtaagttt gattagaaaa tagaaaaaat gcagatgaaa aataaaacgc
    28441 tttccttatt gtgattttat tcactaatgc tgttaatatg cggattggca taatttcctt
    28501 tcatcgtgaa aatttgtgct tttagcttgt atttctcata aatccatgat aatttattaa
    28561 tgaagacggt taaatagtga tacagtttta ttcactgttt caatcgcaga atgaagagat
    28621 tttcttatag atgagagagg aatgtgtgac aaatggcaaa caaaaaagca ccgatcgact
    28681 tccaagcact gctagatgaa gtcaatgcag atttcccagt atatcaagca cttgatcaag
    28741 atggaaacgt agttaacaaa gatttagtac ctgatttatc cgatgatgaa ctcgttgaat
    28801 taatgactca tatggtatgg tctcgtgtat tagaccaacg ttcaacagcg ctgaaccgcc
    28861 aaggacgcct aggctttttt gcaccaactg ctggacaaga ggcaagtcag ctggctagtg
    28921 cttacgcatt tgataaagaa gatgttcttc ttccaggtta tcgggatgta cctcaactag
    28981 taaaacatgg tttaccttta tctcaagctt ttttatggtc tcgaggacat gctgcaggta
    29041 attcttatcc ggaagaattg aaagctctac caccacaaat tatcatcggt gcacaatatg
    29101 tacaagcagc cggtgttgca ttaggtttga agaaacgaaa caagaaaaat gtcgtcttca
    29161 cttatactgg tgatggcggt tcatcacaag gtgattttta cgaagcaatc aatttcgctg
    29221 gcgcttacca tgcaaatgcc gtattttata tccaaaacaa cgggtatgcg atctctacac
    29281 ctcgtgaaaa acaaactgca gcaaaaacac ttgcacaaaa agctgtcgca gctgggattc
    29341 ctggtattca agtcgatgga atggatccgc ttgcagtata tactgtatct aaaatggcac
    29401 gggaatgggc gatcagtgga aatggaccag tcctaatcga aacactgact tatcgttatg
    29461 gaccacatac actatctggt gacgatccaa cccgctaccg tacaaaagac acagatgacg
    29521 attggcagaa aaaagatcca ttgatccgct tccgtaaata tttgacggaa aaaggacttt
    29581 ggtctgaaga aaaagaagaa caagtcatcg aagcaactaa agaagaaatc aaagcagcta
    29641 ttgctgaagc cgacaaagta ccaaaacaaa aggtttcaga tttcttgaaa aacatgttcg
    29701 aagtatctcc acaatcaatc aaagaacaaa ttgcaattta tgaagcgaag gagtcgaaat
    29761 aatcatggca caaaaaacaa tgatccaagc aatcacagat gccttagcac ttgaactaga
    29821 aaatgacgag aatgtcgttg tttttggtga agatgttggg aaaaacggag gagttttccg
    29881 ggcaactgaa ggattgcagg aaaagttcgg agaagaccga gtattcgata ctcctttagc
    29941 tgaatctgga atcgcaggat tgagtttcgg tcttgcgtta gaaggatttc gtccagttcc
    30001 tgaaatccaa ttctttggtt ttattttcga agcaatggac gaagtagtag ctcaaatggc
    30061 tcgtacaaga tatcgaatga gcggcactag aaatctaccc atcacaattc gttcgccatt
    30121 tggtggcggt gttcatacac cagaattgca ttcggataat ttagaaggtt tgatcgctca
    30181 gtctcctggt attcgagtag tgattccttc caacccttat gatgcaaaag gactattgat
    30241 ttcttctatt agaagtaacg atccggttgt tttcctagaa cacatgaaac tatatcgctc
    30301 attccgagaa gaagtgccgg atgaagctta cgaagttcct ttggataaag cagcagttac
    30361 tcgtgaggga acagatgtat ctattatcac atacggagca atggtgcgtg aagcaatcaa
    30421 agcagcagac aatcttgcaa aagaaaatat atctgttgaa atcattgatc ttcgtacagt
    30481 tgcgccatta gatgtagaaa cgatcattca atccgtggaa aaaactggtc gcgtagttgt
    30541 tgtccaagaa gcacaacgac aagcgggcgt tgccgcgcaa gtagtttctg agatctcaga
    30601 acgcgctatc ctttcattag aagccccaat cggacgagtt tctgcacctg atacagtctt
    30661 cccatttgga caagcagaaa atgtctggtt gccaaatgca aaagatatcg aagacaaagt
    30721 aaaagaaatc gcggaatttt aagaaaagaa gggacgaaac aaagtcatgg cttatcaatt
    30781 taaatt
//
LOCUS       DARBDV010000025        30094 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000025 DARBDV010000000
VERSION     DARBDV010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.025"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(4..582)
                     /locus_tag="SCM56_001076"
     CDS             complement(4..582)
                     /locus_tag="SCM56_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEG1109892.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(659..1189)
                     /locus_tag="SCM56_001077"
     CDS             complement(659..1189)
                     /locus_tag="SCM56_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEG1109893.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1193..2053)
                     /gene="rlmB"
                     /locus_tag="SCM56_001078"
     CDS             complement(1193..2053)
                     /gene="rlmB"
                     /locus_tag="SCM56_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEG1109894.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2046..2441)
                     /locus_tag="SCM56_001079"
     CDS             complement(2046..2441)
                     /locus_tag="SCM56_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEG1109895.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2438..3850)
                     /locus_tag="SCM56_001080"
     CDS             complement(2438..3850)
                     /locus_tag="SCM56_001080"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEG1109896.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(3847..4383)
                     /gene="cysE"
                     /locus_tag="SCM56_001081"
     CDS             complement(3847..4383)
                     /gene="cysE"
                     /locus_tag="SCM56_001081"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEG1109897.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(4469..4708)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(4705..6165)
                     /locus_tag="SCM56_001082"
     CDS             complement(4705..6165)
                     /locus_tag="SCM56_001082"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEG1109898.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6335..7492)
                     /locus_tag="SCM56_001083"
     CDS             complement(6335..7492)
                     /locus_tag="SCM56_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HEG1109899.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7526..8896)
                     /gene="radA"
                     /locus_tag="SCM56_001084"
     CDS             complement(7526..8896)
                     /gene="radA"
                     /locus_tag="SCM56_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEG1109900.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(8959..9486)
                     /locus_tag="SCM56_001085"
     CDS             complement(8959..9486)
                     /locus_tag="SCM56_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEG1109901.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            9716..10522
                     /gene="proB"
                     /locus_tag="SCM56_001086"
     CDS             9716..10522
                     /gene="proB"
                     /locus_tag="SCM56_001086"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEG1109902.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            10519..11766
                     /locus_tag="SCM56_001087"
     CDS             10519..11766
                     /locus_tag="SCM56_001087"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEG1109903.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            11842..12186
                     /locus_tag="SCM56_001088"
     CDS             11842..12186
                     /locus_tag="SCM56_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109904.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(12303..12629)
                     /locus_tag="SCM56_001089"
     CDS             complement(12303..12629)
                     /locus_tag="SCM56_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109905.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(13244..14446)
                     /locus_tag="SCM56_001090"
     CDS             complement(13244..14446)
                     /locus_tag="SCM56_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1109906.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            14563..15078
                     /locus_tag="SCM56_001091"
     CDS             14563..15078
                     /locus_tag="SCM56_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEG1109907.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(15277..15723)
                     /locus_tag="SCM56_001092"
     CDS             complement(15277..15723)
                     /locus_tag="SCM56_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HEG1109908.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(15770..17248)
                     /gene="msr(C)"
                     /locus_tag="SCM56_001093"
     CDS             complement(15770..17248)
                     /gene="msr(C)"
                     /locus_tag="SCM56_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HEG1109909.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(17602..18231)
                     /locus_tag="SCM56_001094"
     CDS             complement(17602..18231)
                     /locus_tag="SCM56_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEG1109910.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(18215..18679)
                     /locus_tag="SCM56_001095"
     CDS             complement(18215..18679)
                     /locus_tag="SCM56_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG1109911.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(19166..20434)
                     /locus_tag="SCM56_001096"
     CDS             complement(19166..20434)
                     /locus_tag="SCM56_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEG1109912.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(20665..21402)
                     /locus_tag="SCM56_001097"
     CDS             complement(20665..21402)
                     /locus_tag="SCM56_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG1109913.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(21469..22038)
                     /locus_tag="SCM56_001098"
     CDS             complement(21469..22038)
                     /locus_tag="SCM56_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEG1109914.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            22166..23038
                     /locus_tag="SCM56_001099"
     CDS             22166..23038
                     /locus_tag="SCM56_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG1109915.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            23380..24252
                     /locus_tag="SCM56_001100"
     CDS             23380..24252
                     /locus_tag="SCM56_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG1109916.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            24249..26207
                     /locus_tag="SCM56_001101"
     CDS             24249..26207
                     /locus_tag="SCM56_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1109917.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(26539..27390)
                     /locus_tag="SCM56_001102"
     CDS             complement(26539..27390)
                     /locus_tag="SCM56_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG1109918.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            27513..27980
                     /locus_tag="SCM56_001103"
     CDS             27513..27980
                     /locus_tag="SCM56_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEG1109919.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(28243..28725)
                     /locus_tag="SCM56_001104"
     CDS             complement(28243..28725)
                     /locus_tag="SCM56_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HEG1109920.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(28825..29688)
                     /locus_tag="SCM56_001105"
     CDS             complement(28825..29688)
                     /locus_tag="SCM56_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEG1109921.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         8586 a         6370 c         5452 g         9686 t
ORIGIN      
        1 aaattattcc tcattataaa atttcaagtg tttttttatt ttgagtttta ctctcccgta
       61 agcgctcatt acttgcttgt gatccattgc taaacattca gccatgtcta caaaatcaac
      121 gccttgcata taataatgaa aaattttagc ttctattttt gaaaactgga tttcgcaatt
      181 ttcgattgct tcttttaata aaagatgctg aattgctaaa ggttcttgca atgtgatctc
      241 tgctaaaaag tctggtcctt ctgtctctat tttttggtcg attgagactt catctttttg
      301 atcacgacgc ttcagcgcgt tctgcttcct caacagagaa tagatttgat tgcttataac
      361 gcttttataa taacttccaa agctaacacc tttgcttttt tcgtaagttc tcaatgcctg
      421 atataaagcg atccttgctt cttgttccca atcctcagaa acataatttc ttaaataata
      481 ggtgtttttc aaacgtagta aaagtggaaa atatttttga tacagggcag caaatgcttc
      541 ttcgtcacca ttacgtgcag cgcgcaataa ctcctcagac ataaaataat tcctccataa
      601 attcgtttat ggaggaatct tatcatcaat gattcatcga actgattaga acgatatgtt
      661 atttattttt tctcattaaa tcgtccaatt tttgggacaa tttttttaac tgctcttcat
      721 tccatggaga attacgtcgg aaatcaataa aatgcatatc tttattttct tgggctattg
      781 ttttttccgt tttttttatc gtcttatata attcataagc agaagttcgt aatgctcctt
      841 gggaaaagat catccactgt tctgctaaat cgcttgtcgc cactgtcact tgtgtcaaac
      901 ggtcattcag ctgaccagcg attctctcga tataactgtc agccgtttcg tcttctttcg
      961 taaaaacaac atccaactgg tatttcttat aattttgttg gatgcccgga actagttggg
     1021 catcgaagac aacgatgact tccattcctt catatttcgc ataatttgat aaacgatgta
     1081 aaagagcttc tcttgcatcc gccattttgt cctgcttttt aagctggaca agttccggcc
     1141 acgaaccaat catgttgtag ccgtctacaa tcaacagctg ttttttcatg gtctactccc
     1201 cctgacgttg ggaaaaagcc tgatacataa gcaaaccagc agcaacactg gcatttaagc
     1261 tctggacgtg cccggtcatc gggatcgtca gcaattcatc catttctttt ttcaatcctg
     1321 gactcattcc tttgccttca ttaccaatga ctaaagcaat tgatcctttg gcattccatt
     1381 tttggaagtt tgtgccggtc atatctgtgc caaataccca aaatcctgtt tctttgagct
     1441 gttgcacagc ttgtaccaaa ttcgtcacgc gtgcaacagg gacatgttcg actgccccag
     1501 tggaagtctt tgttacgata ggtgtgattc caactgctcg gtgttttggt atgatcacac
     1561 catcgacgcc acttgcgtct gctgtccgta aaatcgaccc aaaattgtgt ggatcttcga
     1621 tgctatccaa aatcaaaaag aaggggtctt cttttttctc ttttgttcgt tcaatcagtt
     1681 ggtcaaggga taaatattca taaggtgtaa tcgcaagaac gactccttga tgcacgccgt
     1741 gatcgctcaa agtttctaat ttttgtttag gaacccattt gactggcaca gcttgttcac
     1801 gtgcgatttc tttcagttct tctacttttt ctcctcgtga atcttcttga agaaagagtt
     1861 tattccctcg cccttgttgt agcgcttcta ctgcggcatg gtgcccaaag acgaaattat
     1921 cttctaatgc ttcttcattt tgatcagaaa ctgactgatt tcgtccagtt tgctttttat
     1981 ttgaatatct attttgtttt ttgtcttcaa atccacgttt cgattgatgt ttgtttgttc
     2041 tttctttacc catttttttc accaactttc tgaatacacc agcggatcaa ttcttccatc
     2101 cgctcggttt gttttgtcaa atgcagatag cccatgagcg cttcgaaccc tgttgcgacc
     2161 cgatacgtcg taacatcagc attttttgcg ctcgtatggc ttttagcatt tcttcctctg
     2221 cgataataaa gttcttcttc ttctgtcaaa agatgttctt ccagcatttg ctgaatcaaa
     2281 aaggcttgtg cattcgcaga aacataattc gttgccgcac gatgcagctt attgggtttt
     2341 gtttctcctt gatcaatcag gtggtcacga acatagattt cgtaaatcgc atctccaaca
     2401 taagccaagg ctaaaccgtt caattgtgtg taatctctca tgcttctctt ctccatcggg
     2461 ttccttgcgg tgtatcttct aataagatcc ctttttcttt caacaaatca cgaatttcgt
     2521 cgcttcggac aaaatcacga ttgcttcttg cttgatttcg ttcttcgatc aattgttcaa
     2581 tctcttcatc taataactct tctgctgtaa ataaaatacc gaagatcgaa agccattgcg
     2641 agaataaagc ttctccttgt gccaagacag cggcagaaac tttcggctgg tctagataag
     2701 tgttcagcca cttagcaagc tcatagacca ctgtgatccc atttgctgca ttaaaatcat
     2761 cgtccatttc ttcgatgaat cgctgttcta aattttctaa caaagcgatt ttatccgcat
     2821 catctgccaa attctctgag gcagtttctt ggcggaattt caaattgtca aacgtattct
     2881 tcagtcgttg taaattggtg gctgcttctt tcatcgtcgt ttcactgtag cggatcggac
     2941 gacggtattg tgtggtcgcc atgaagaaac gcaagacttg tggatcaacg tttttgatca
     3001 tttcatgtac agtgatgaaa ttccctagag atttactcat tttttcatcg tcttcgccaa
     3061 tcgttacata gccattgtgc atccagtaat tagcgaattt tttccctgtt ttcgcttcac
     3121 tttgtgcgat ttcattttca tgatgaggaa attctaaatc ttgcccgcca ccatggatat
     3181 cgatcgtttc tcctaaatgt tttgttgcca taacggaaca ttcaatatgc caaccgggac
     3241 gtcctttgcc ccatggtgaa tcccaagaaa tctcgccagg ttttgcttgt ttccataaag
     3301 cgaaatctaa tgggtcttct ttttgctgct gttcttcccc tgtacgctga cttgctccga
     3361 cttccagttc atcgatggat tggtcactta atttcccata attttcaaat ttacgggtac
     3421 ggtaatacac atcacctgaa gattcataag catagccttt ttcaaccaac acttgaataa
     3481 atgctaaaat atctggcata tgatccatga ctcttggatg cagtgtagca ggttcgacat
     3541 tcaacgcttt cgtatcttct tcaaatgctc ggatgaaacg atcagcaact tctggtgctg
     3601 taatacctaa ttcgtttgct gtacggatga ttttatcgtc tacatccgtg aaattcgata
     3661 cgaaatcgac tttatagcct cgatattcaa aatagcgtcg aattgtatcg aaagcaatgg
     3721 tgctacgagc atttccgata tggatataat tatacacagt cggtccgcag acatacatac
     3781 cgacatgtcc cttttgtata gggatgaatt cttctttttt tctggtcaat gtattgtaga
     3841 tttttatcat tctgatctcc tcactttttc tcctttgatt cgaacgactt ttgctggtac
     3901 tcctaccgct gtagcgtcgt ctggaacatc tttcaacaca acggcacctg caccgatttt
     3961 ggcgtttttt ccgatcgtta ccggtcctaa tatctgcgca ttcgcagaga gcatggctcc
     4021 ttgtttaaca gttgggtgtc ttttacccgt ttcttttcct gtgccgccta atgttacgcc
     4081 atggaataaa atcacatcgt cctcgatttc tgcggtttcg ccaatgacga ttcccattcc
     4141 atggtcgatg aacacgccag ttccaatcgt tgctcctgga tggatctcaa caccagttag
     4201 gaaacgccag aattgagcat tgatcttcgc caataaaaag agccggtgct gatataaaaa
     4261 atgagagaaa cgatgccaaa acaaggcatg aaaaccaggg tatgtcaaaa cgatctcagc
     4321 tgtcgaacgt gcagccggat cgtttctctt tgctgcatca atcgctcggg taaaccaact
     4381 catgactttc cactcctttc gtaagaattt tatgtaggaa atcatcttct tataaagaaa
     4441 agaagcgcct tttaaataag tttccttatc aaaagacgct ttccgcgtgg ttccactttt
     4501 cttcataatc aatgattatc tctgacagct aacgcctgtt atacgttctt ggctactttg
     4561 tttcctataa aacagttttt cctgttttct gtgtttcgcc aagaccactc ccagaggcat
     4621 ttcggcaacc attcattgat accttgcacc acccggtatc tctcttgtaa tgatcagctg
     4681 cttacttttt tctgttcatc gtgtttagtt cagtacttga tttaaatgtg ctaatgattt
     4741 ttcacgacca agcaattcga tcgtttcagg caattctgga ccatgcattt gtcctgatac
     4801 agctacacgg attggcatga aaagattttt tccttttacg ccagtttctt tttgaacagc
     4861 tttgatggca gctttgacag ttggagcatc tactacttcc atttgttcta gctgtgcttt
     4921 gaatgcgtgg agaacagtag gaactgtttc accagcaagt acttcttttg ctgcttcatt
     4981 caatacagga tgttcattga aaaatagcgt agacagatca acgatttctg cagcatagct
     5041 catttgaggt tgatacaggc taacgatctt cttcacccat tcgattgtct tttcatctgg
     5101 gttttctgct acacgtccat cagcgattag ataaggcaga cacatgtcag taaatgcatc
     5161 tagatccatt gctttgatgt aatggttgtt tacccattgc agttttttcg catcgaaagc
     5221 tgcgggtgat ttacttaggc gttttggatc gaacattttg atcaattctt ggtgttcgaa
     5281 gatttcgtct tcgcccacag gtgaccagcc caataatgca ataaagttga acattgcatc
     5341 tggtagatag cctaattcgc gatattgttc gataaattgc aagattgatt cgtcacgttt
     5401 gcttagtttt ttacctgttt cactgttgat gatcaatgtc atatgtccga aggttggtgg
     5461 tgtccagcca aatgcttcat agatcatcag ttgttttggt gtattggcaa tatggtcatc
     5521 tccacgtaaa acgtgagtga ttttcatcaa atgatcatcg accgcaacag caaaattata
     5581 agttggcata ccatcgcgtt tttggatgac aaagtcgcca ccgatattat cggattcgaa
     5641 cgaaatagcg cctttcacca tatctgtaaa tgcgtattct gtattgcgag gcacacggaa
     5701 acgtacaaca gattctagac cttgtgcttc tttttctgct tgttcttcag gtgtcaagtt
     5761 cgcacatgtt cctgcgtaat gaggcatttc tcctcttgcg cgttgtgctt cccgttctgc
     5821 ttctaattct tcttctgtgc aatagcattt gtatgctcga ttgctagcaa gcagctgatc
     5881 aatcagcggt tgatagattt ctttacgttc agattgacgg taagggccgt attctccagg
     5941 attttcagga gattcatccc aattcattcc caaccaagca aggttttcca actggctttt
     6001 ttcgccatct tcgatattac gtttttggtc agtatcctca atacgaataa taaagtctcc
     6061 atcattgtgg cgggcaaaca aataattgaa taatgcagtt cttgcatttc caatatgcag
     6121 gtgcccagtt ggacttggtg catagcgtac gcgtactttc gtcattttct tcctcttctt
     6181 tcaaactaga ctttgactaa cagcaaaaat tccgtcagtt acggcttctc aaatatcatt
     6241 tatttaaagc cactcagcac aaattttacc ttttctaagg taaatagtaa agccaactgg
     6301 ttgaatttcc ttaccagttg gctgaatttc ttttttattt tgattgttta gtctttttct
     6361 ctgtctcttt tttattgtcg attcctcgtc ctgcatgtgc aggtttagca aagatcatgc
     6421 gtccagcagc tgtttgaagg gcacttgtta caacgacttc gatacgttca ttcatgtaat
     6481 gctgtccatc ttctacgaca accatcgtgc cgtcatcaag gtaggctact ccttgttggc
     6541 gttctgtccc ggcttttact actagaacat tcatcgtttc accagggatg acgactggtt
     6601 ttacggcatt tgccaacgca ttgatattaa gaacaggtac attttggaat tcagagactt
     6661 tattcaagtt atagtcattg gttacaatga caccatctaa aagcttagcc aatttgatca
     6721 atttgctgtc tacttcactg atatcttcaa agtctccatc gtacatttcg acagatatac
     6781 cctcttcttt ttgcaaggca tttaggatat ctaatccgcg acgaccacgc acacgtttca
     6841 aactatcccc tgagtccgcg atgtattgta gttcgtaaag aacaaaatta gggatcatca
     6901 aggttccttc taagaatcca gtctttgtaa tatcataaat ccgaccgtca atgatcacgc
     6961 ttgtatccaa gattttatat ttatggaaat tttctcctga ttttcgttct aacagttgac
     7021 tctcaacttg actagctgtt tcttgctcga tttttttgct tcgtgaacca aaaactttct
     7081 tccattcttc gattcgtgtc gttcccattc tcaatcccag atagcctaag atgatcatca
     7141 gtaaaatcgg caatacacta ttgacgaatg gaatttccat attgtaaaga ggggtcgaga
     7201 tcacaacacc taaagtcaat ccgatgatcg caccgatcgc accgaagaga agataagtca
     7261 agctgacctc acttagtttt tgttctactt gtttgataat agctgagata tgtttggcca
     7321 acagcaaaga taataaaaag aaaataagtg cgcctaacag gctgttggta aatgtattat
     7381 tcagccatcc gtttgactgt tgaccgattg cctgccaagc tgagggtaaa aatgaaatac
     7441 ccaagcttgc gccgacaatg atcatcagca aagtaatgac acgtttttgc atcgtttttc
     7501 ctccgtttcg acaatcattt attagttagc ggaacacacg cttcaacgtt tcagccaaag
     7561 ttgctactcc aactatctcg attccttttg gcgcttccca gttacctaag ttgtttttag
     7621 gtaggtatac ttttgtgaag ccgagttttt ggacttcttt gactcgttgt tcaatatgac
     7681 tgacacgtct gatttctcca gtcagtccaa tttcaccgat gaaacattcc gttggctgtg
     7741 ttcctttttc tttatagctt gaagcaatac tgacagcgat agctaaatca atcgctggtt
     7801 catttaattt taccccacct gcagctttta agtaagcatc ttgattctga agaagcaacc
     7861 cagctctttt ttccagcact gccatgatca gagatacccg attgaaatca agacctgttg
     7921 ttgttcgttt ggcatttccg aacatagtag gtgtgactaa tgcttggatc tctaccaaaa
     7981 ttggacgtgt cccttccatt gccacaacga tagcagagcc agttgcaccg tctaggcgtt
     8041 cttctaagaa aatctgtgaa ggattagcga cttcttccaa tccatgctca tgcatttcaa
     8101 aaatcccaat ctcattcgtt gagccaaatc ggttttttac tgctcgcaaa atccgaaaac
     8161 tatgatgttg ttcaccttca aaatataaca ccgtatccac catatgttct aacattcttg
     8221 ggccggcgat cgagccttct ttggtaacat gaccgacgat aaaaatggct atcccatttg
     8281 ttttagcggt tttgagtagt tctgcagtgg tttctcttac ttggctgaca cttcctgcca
     8341 cacttgtgat gtctggctgc gtcatcgttt ggatggaatc aatgataaca taatcagggg
     8401 agatatgttc gatggcacga ctgatttcgt tcatatctgt ttcagcgtat agataaaatt
     8461 ctgtgttgat acttccgagg cgttccgcgc gtaattttat ctgctctgcg ctttcttcgc
     8521 cggatacata taagactttt ccgcctatgg ctgctaattg ctgagaaact tgtaacagca
     8581 aagtggattt cccgatacct ggatcgccac caatcaagac catagaccct ggcaccacac
     8641 ctccgcctaa cacacggttc agttcttcta attttgtttt gatccgaggt tcttttttag
     8701 ggacgacgtc tgcgattttt gttggttttg ctttttctcc tgtcaaactt gtccgagttc
     8761 tgcggtccgt tgtatcttgt tcgatttctt cgaccatcgt attccatttt ccgcaatttg
     8821 ggcagcgtcc caagaactta ggcgacacat acccgcaatt ttggcaaaca aattgtactt
     8881 ttgatttttt tgccattgta ccgcctcctt ttatcctaat catgagacat taaatcaagc
     8941 ttaagtatct ttaacgtttt atttactgga agaaccaaat ccgccttgac gttgagcagt
     9001 cgtttgatct tgatctgctt ttaaaaacgg caaaaagatc ccttggccga tacgctcgcc
     9061 ttttttgatc actacatcct tgaaaccaaa gttggtgaat tggaacatga tatggccttc
     9121 atttgcctca ttattgtaat aatcactgtc aatcacacct actccgtttg tcaacactaa
     9181 aaaccgcttc aacggattgc ttgaacggtt agcaagctgc aaaaattcat cttctcccat
     9241 gtaagctttg attccagtag gcacgagggt tggtttcaat tgtttttcat tttctgatac
     9301 atcttttttc aatccatctg cgactgtttc caaccccttt ttccagcctg ctttccagat
     9361 acttggaatc acgacctctt ctgctgcttc aaaatcatag ccagcagcat gatgtgtagc
     9421 gcgctgcggt agattgatat tttgatcttt atatttagaa ataacttcaa atcctctttt
     9481 tctcaccggt tttcctccct aaataaacta ctcctattct aacatatccg aggctgtaaa
     9541 agactttctt tacaaattct tcaagaaatt ctgctatctt aaagtaaacc attaattcgt
     9601 tctttttcca aacgactgca agttgtacaa aaaaactatt ttttacatga ctttactcgt
     9661 caaaacaagt agaatagaac gaaattaatg agtggatatt tgattgaggt gtcacatgcg
     9721 taaagaaatt aaaaatgcaa aacgaattat tgtcaaagta ggaacaagta cattgattta
     9781 tcccaatgga aatatcaatc ttgcggcaat cgatcagtta gcttttgtcc tttctgatct
     9841 tagaaatcgg ggaaaggaag tgatcttagt ctcatcagga gctgtaggcg ttggtatgca
     9901 taaattacat ttgtcagaac gtccgacttt gattcctgaa cagcaagctg cggcggctgt
     9961 tggtcaagcc gaactgatga atatctataa tcaaagattc agtgtatata atcaacagac
    10021 ggcacagatc ctcgtgacaa gagacgtggt agattatcct gaaagtcgta aaaatgtgag
    10081 gaatacattg gaacagcttt tgattatgga cacgatccct atcgtgaacg aaaacgatac
    10141 tgtagcagtg gatgaattag atcatctgac aaaatttggc gataatgatc agctgtcagc
    10201 catcatggca aaacttgtcg cggcagacct tttgatcgtc ctttctgata tcgacgggtt
    10261 ttattcagat aatccgacta caaatcccga ggctcagatg tatcgctata tcgataaaat
    10321 cgatgaaaag attctggcac aagcaggtgg tgctggtagc acatttggaa caggcggtat
    10381 gcagagtaaa ttaaaagccg cttctaggat atttgaagca aacagcgcaa tgattttagc
    10441 aaatggaaaa aatccggcaa ttatttttga tattcttgca ggaaaagaag taggtacttt
    10501 atttaaggag gacttgaaat gactgacttg atgcaattag gacaacaggc aaaagatagc
    10561 gcgcaacaac tagcacttat gcctacaaac cgcaaaaatg agctacttgt tcaaatggca
    10621 caaaccatca aaaagaatca acaagcgatc attgaagcaa atcataaaga cttagaaaaa
    10681 gcagtcgaaa acaatatcag tgaaacgatg caagaccgct tgcagctgac acctgagcga
    10741 atcaatgcca tggctacaga gatcgaaaaa attgcttcgt tagacgatcc tattggaaaa
    10801 gtagatgaga tgtggacgaa caccgacgga ttgagaatcg gcaaaaagcg cgtgccacta
    10861 ggcgtgatcg gcatcattta cgaatctcgt ccaaatgtca cgacagatgc agctagtcta
    10921 gcattcaaat cagggaatgc cgttatcctt cgcggtggca aagaagcatt cttctcgaat
    10981 caattattag tccaattatt gcagcaagta ctggtcagtg ctggcgaatc tccttatgcc
    11041 atccagtttg tcgatgatac ttctcatgaa actgcaggac agttgatgcg attgacagaa
    11101 tacttagatg tgctgattcc tcgaggcgga gcaaaattaa tccaacgagt caaagaacag
    11161 gcaacggtac ctatcattga aacaggtaca ggaaataatc acgtatatat cgataaagaa
    11221 gcacagttat ctatggcagt gaacatcatc gtcaatgcaa aagcttcccg tccatctgtc
    11281 tgtaacgcgg cagaaacatt gctgatccat tctgagatcg cacctttttt cttgccagct
    11341 attgaaaaag aactggtaga acacggggtc tctttacgtg cggatgctcg tgcgttagaa
    11401 tatctagaga cggctgcttt agcagaagac acagattggg atactgaata tctagattat
    11461 atccttgctg tcaaagtggt cgattctttg gatgaagcaa ttgcacatat caatcggcat
    11521 aatactaaac attcagaaac gatcgtgaca gatagttatg cggctagtca acgtttctta
    11581 aatgaagtgg acgcggcggc tgtctatgtc aatgcctcca ctcgttttac agacggatcg
    11641 gtatttggtt ttggtgcaga gattggtatc tctactcaaa agttgcatgc ccgtggtcca
    11701 atggggttaa atgaattaac ttccacaaaa tatatcattt atggcgatgg acaaatccgc
    11761 gaataatgtt tcgtgtgaaa cattattccc gccttttgcc ttttttctgt aaaggttcta
    11821 tccaactaga aaggtgtcaa tatgtttgaa attttaatga ttttttttat ttaccttatt
    11881 tctctgaata tcgctgcttt tctgggagtc ggtattttat cccttttctt tcaattcaaa
    11941 aaacgaagca tagggagtca acgggaaaaa tggtcgcagt attttgacaa aatcgggcaa
    12001 aaagggctgg ttacacgctt gcacattagt tacatggttg cattatgctt attagcgaca
    12061 atcaattact attctttttt cgatcattca atcgcttata cgattacgct actgattgct
    12121 gggatttttc acttatctta taaatatcag ctgaataaaa accacttgaa tcggactttc
    12181 cgctaaaaaa tatccgtacg ataagtagag tgccttgaag atcggcaaaa aactgccgga
    12241 ctttttagag gctgtgacaa agattttgtc acagcctctt tttccatata aacggcgttc
    12301 gcctaccagc tgcctggtaa atctgtttcc tcttcgttaa gaagcgaaaa tccgtcaaat
    12361 gtttcgccat cataataacg aagcagctca tccatggaca agttcatcgt tttttctttt
    12421 tcttcttccg ctgattcagc cagtagctgt tcccgataca cctgatacat accagctatt
    12481 ttttgctgca tcgcttttct agtttcacct tccgcattga ttattggcaa ttctttgata
    12541 tagccacgat aaatcaaccg atcatctgaa aattcaatgg gtaattcaac gagtgtcaac
    12601 ccgacttcct gaaaatattc ttccagcatc tattatcccc ccattctttt tcttaaatga
    12661 attttagctt acctactgaa aattttccag tttcattatt ttgagaaaaa aagggaatgt
    12721 gacaaaaatc gtgtcacatc ccctttttcc gaataaatgg tgttcttctg ccaactcctc
    12781 aatcataagt cacaatattc aacaaacatg agaaactgtt ttttgaaatt gttccttgtg
    12841 attcagagtt aaacgacagt ctcacaacct cttggtaaac ggcgttcaat cagctgaccc
    12901 tcggtattag ctcaaaaaac ggtaaaatat gagaagctat ttttccgttt tgtttctcta
    12961 atacttagga taaaacgctg attttacaac ctctgttaaa cggtgttcaa tcagctgtcc
    13021 ttcggtatta gctcaaaaaa cggcaaaata tgaggaacta tttttccgtt tgtttctcta
    13081 atactcagga caaaacgctg attttacaac ctcctgataa acggtgttca aaagcttgca
    13141 tctgcggtaa taagcccaac caaacgaaaa atatgagaag ctatttttgt ttggctgttc
    13201 ttatgacttg atgcaggaca gctttttcac aacctctgat ttcttattct tcttcatttg
    13261 tagcaaaata attacggtga taaatgatac aacatacact gataagaaca atggcacctg
    13321 caaaataaaa cgcatagctt tcaccaatat gttcaccaat cacacctgca ataaatggtg
    13381 ctaaagctcc accgaaaaag cggacaaaac tatagctgga cgaagcaacg tttcgttgca
    13441 gacctggtat ttccatagca atcgtcgtca acagtgtatt gataattcct tggaaaaatc
    13501 ctgctaacac aatgctgatc ggtacaagga ctggcataga agttccagct cctaataaca
    13561 gcaaacaaat aatgaataag acaaatgctg ctaaaattgt ccgataagtc gtaaacttcc
    13621 gctcaagagc cggtgcaacg aaaatcgaag caatagccag aagcacaccc cagccaaaaa
    13681 atacaaaacc gacttctaat tcagaatagc caatcaataa aaatggcgca tacgcaagta
    13741 aagtaaagaa tccgaaatta tataacaatg cgattagtcc aactgaacga agtttatgat
    13801 tgcttaacgc tttgacacct tcccaaaggc ctacttttct ctttggtttc tcagtttcag
    13861 gtaaaagtac cgctagggct ataaaaccgc aaaacatcaa aaatgctacc ccaaaaaatg
    13921 gataacgcca tgaaccactg cccaagaccc caccgactag agggccaaca gccattccgc
    13981 agccaagtgc tgcttcatac atcatgattg ctttttctgt gttaccaact aaaatactga
    14041 cgatcgttgc taaagcagta gagacaaata aagcattccc gacaccccag ccggcacgta
    14101 acccaatcag cattccgata ttcccagata atccgccaag accagcgaat acaacgatga
    14161 taaacaatcc tgtcatcaat gtttttttca aaccgatccg actagataag aaacctgtaa
    14221 atagcatcac cacaccagtg actaacatgt aactagtaaa taacagggtc gtttcagctg
    14281 gcgtagcgtt taactgtgca gcaatcgttt ttaagattgg gtctactaga cccactccca
    14341 taaaagcgat catacaggta gctgctacaa ctagcgctga acttttcccc tttgtttctt
    14401 catttgtttg ttcttgtata tctatttgat tctccaatat ttccatccct ttctctctat
    14461 tagcatcata catgatttat tttacatgtc aatatgcaca tgtaaaacgt ggaaacttta
    14521 ctgtgatata ataaagaaaa taagacgaga ggtaggattt ttatgaatac tttagcctat
    14581 atattattat gcgtgctttt atccaaacca cgttctgggt atgaattgaa gcagctgatc
    14641 acaatttttt gggaagccca tcacagtcag atctatacaa cgcttgccaa gttagaccag
    14701 cagggttatg tgaccgtttt ggacaaagac gagcactctc aaaaaaagat ctaccacttg
    14761 actgatgaag gacgtgctct tgtagaggaa tggatcaaag aggaaactcc cgctcctacg
    14821 caaaaagatg aatttctagc aaaaatctat gctttttcga cgctcgataa aacaacagct
    14881 actggactgc tattcactag agaacaacag ctgaacaaaa tcatgcaaaa aaatcaaaaa
    14941 aaattgaatg acctctcgct agacaagaaa caagaatttg gacgttatgt tgtgatccag
    15001 cgcagaatta tgctttgtca gcaggaactc caatggtgtc agcaagtaag agaacaaatg
    15061 cagcagtttt ttgagtaagg gataaacaga acctttctag aaatatctat tgaagagcaa
    15121 attatcttca ataaaagagc aatacagttg gaaaagaaaa agcgatccga acgacaggcc
    15181 aaaacctatc gttcggatca ctatttttct agaactgcat tcaagagact atgacaaaaa
    15241 gcatgccaag gccttttttc aaataacagt gttccatcat tcattacttt gaattgcttt
    15301 tagcacactt cttgaaagcg ggccaacgat gatcaactgc aacggtaaag cggcaatcac
    15361 attcattttc caagcattca tataagcaga gaataattga tcaggtattc cttgttccat
    15421 gatcacaccg aataaagaca tacatgtcac cattcctaaa accatcaagc atgaaatagt
    15481 taagatcatt tggaccttgc tttctggatt tactggtagt ttaaaggcaa tttttttcgc
    15541 cagcacaccg atgatgaaga catccaaaat gaaagccacc acgaatccag gcactagacc
    15601 aatcaacaaa cttgataaat gaagatcttg atgcagccat aaattatacg cgctcatccc
    15661 caagaccatc aaaaaacaca tcattgttgt aaaaattatt ccttcttttt tatttgtagg
    15721 caatctgtac cactccttta aatttgtcac gaaaatcatc gtagcacatt taaaaatttc
    15781 tcgtaagtac tttttttatt ttctgccctt ttatttcata aacttcatca gccgtacgct
    15841 cgacaaagta agaatcatga gaagtaaaca aaatcgttcc cggatatatt tgcattagct
    15901 tctctaaagc ttcgatcgtt gccatatcaa taaaattagt cggttcatct aacagcaaca
    15961 cattacttgg cttagtaaaa agcaaagcaa tcgtcagacg ggtcgcttct cccccactta
    16021 atgtacataa cggtttcgtc aaagtttcct gagcaaaccc taagtgattc agcaaactac
    16081 ggacaagcga ttctgaaaac tctgtttgtc tcatgacaaa acgaatgatg gattcttcag
    16141 atgtcatttg ataacccttt tgctgatata cttgaaagct tactttcgga gagagaagga
    16201 ttccttctcc ttgtttgcgg atatgttcta ataaggttgt cttacctgaa ccattttcac
    16261 cgacaatcgc gattttttta cctaaagaaa actgaaaatc accatttacc agtaatgttc
    16321 tcccacttct ttccaattgg acattttgtc ccatgatggg atagcggctg tggagttcaa
    16381 gagattttgg tactggaaaa cggatttgtt tcatctgctg cggcttggtt gtttctccta
    16441 ttttttggag tcttctctct aatgttttcg cattcttttg gatggctttt tgtacactgt
    16501 ctttttgttt agaggaagac aaccgatcca gtctgatttg ttgctttttc tttttactgg
    16561 acactttttc caacttttcc gcttgttttt tcttttcttg gatcgatttt tccaatcgtt
    16621 ttttctcttt catgatcgac tctgcttctc gtagctgagt ctttttctcc aattcctttt
    16681 gagtgagata ggcactataa ttccccgaaa actctcggat ttctccgtct tttacttccc
    16741 agattttcga tgctagctca tctagaaaat agcgatcatg actaacaaaa accagcgtac
    16801 cataataata acgaagctct tcaatcagtc gatcaatact ttttctatct agatgggttg
    16861 ttggctcgtc tagaagcatc ccttgttcat aagaagaaag aagacgtatc aactcgatct
    16921 ttcgttcttc ccctccgctc catttccgct ctgaagtttc ttccagtctt aactggaaat
    16981 gactggctaa ttccaaattt aggtcctttg cttcagcgag ataggttaat tgaggcaggt
    17041 agttgaaggt gatctctgtt tggattttcc catgatcggg aaaaagttca ccggctatta
    17101 gtttgagcaa agtcgattta ccgacaccat tttctcctat gatccctata cgatcttgct
    17161 gatagacacg aagagaatca atagatagtt ctagctggtt gccaaagctg acttgtagat
    17221 ttgttatatt tactgctaaa ttttccatat gttttacact cctatttcat gtaaaacaac
    17281 aattatcgat actaaaaaag agcagaaaaa tctgtttgca aaaaaactgc atcacatctt
    17341 ttcctactct gaacgaaata actatgacta aatcaccaaa tctgtcatag ctttcaccta
    17401 ttctactgtc gttcgaaaaa atggacatac tccatccatg aaattgttgt ttatacatgt
    17461 cttttgaatt tatttagttg ttattcaaaa gttcgaaacg taatttcatc gatgcagtca
    17521 tttttccgac ctcctttttt attaggttac cacacacgat tcaaaaaaag caatagctac
    17581 tgaatagtaa ctgaaaacct tttattcgaa catagtgatc ttttctggtg ctaaacgaac
    17641 agactcgtaa ccttcttcta ccgcttttcc tattgataaa ataataactg gaacataacg
    17701 gtctttatcc aaaccaaatg cctctgccaa ctgatctgct tcgaatccac caattggatt
    17761 tgtttcatag ccatgatcac gcgcaactaa catgaattgc atagccgcta agctagaatc
    17821 gactttgact acatcgttca tttcttctct tgaaaaactc ttgtaataag gaatgatcgc
    17881 agctaattgt tgatctctga cttcttttgg catcttccct gattcgtaag cttggttgta
    17941 gatttcttcg ccatattcgt aacattgcat atcgccaaaa atcatgacca ttgcagaaga
    18001 agtatcattt tgtctcgtat tgaaacggat caaaggacgc aagatttctt ttgcttcctc
    18061 gctttcagcg accacaaagc gccatggttt catattgaca gaagaaggtg cagtcgtcgc
    18121 ttctttgatc atttccagca tttcttcgtg tgggattttg tatgtttcat catatacgcg
    18181 aacggatctt ctaccagtca taattgttga aaaatcattt ttttgttcca ttattattaa
    18241 cctctttcgt ttctgttgtt atattgcaaa gttgggtatt caatttatct aaaagtgtta
    18301 gaaattgttt catttctaca tcgctgaaag ccggggatag cacggaacct gtatctttat
    18361 ggtccatttc acaatgcttg attttcttct gggctttttc tgttagcgct acatacactt
    18421 cccggttgtt ctgaggattt ctctgacgcg aaacatagcc atttgcttcc aataccttca
    18481 gatgacgagt aactgctcct tgatcaatct gcaacttttc ttgcaaactg ctttgtagca
    18541 atggattttc ttctttcagt ctcatcatga tctcgtatct agtaagactt ataccgatat
    18601 tttcttcaaa gatctggacg attttttgat cggctaattt gatttgatat aaaaggtgac
    18661 taatctcttc taaagacata atttcctcct ttctaataat tgactcgtca acgattgact
    18721 tgtcaataat agaataaaaa aagaaaaaag tcaaagaatc agctgtgtaa taaaaaaaac
    18781 gctctcaata gaaaagtata gagaaaagaa attttttgta gaatgaacgg aaaaaatgat
    18841 aaagaaagca caacgctaaa accaagatta ttgattctta tcaaactata gaagatagct
    18901 cttctgcagt agataatttt acaccgccaa aaatttccga aatcgccttt tgcaagaatc
    18961 ccgtcgaata ttagaaattg gatcagtatg cttaaacagt aataaaaaag ttatgacaag
    19021 atgttgtcac aacctttttt gagtaaacgg tgttcaaaaa cttgcatctg cggtaataag
    19081 ttcaaccaaa caaaaaacat gaaaatctgt ttttgtttgg ctgttcttac tacttgatgc
    19141 agggcagctt tttcactacc tctttttaat aaccataaaa tcctattcct tcatatctcc
    19201 agcccatcgg tgttagccaa tctctttcct tagcactcaa ggtatagtga tgggtaccag
    19261 ttttttcatt tggattaaat agtcgataaa ttggcttgga accaccagaa taaaaactta
    19321 gaccttcata acgccaaccg atttttgtta aagagttgat ctcagattgt gccattgtgt
    19381 aatgatggtc tccactgtaa ggattgtata aacggtagac aggttgtccg ctgtttggtg
    19441 cttgccaagc aatcccttca tattcccagc ctacagttgg cagataattt ttttctccgg
    19501 cattcaatgt atataaatgt tctcctgtat gacggttata cagacgatac atcggagaag
    19561 ttgaagttgg tgctgtatca tactgtgtca gattataggt ttcgattaca ttgttcaatg
    19621 tagaaccata attaggcgca gtggcataac gacctgttag ccaagccgta gcatctttgt
    19681 aagagttcgt attgcttttc catgcaccgg aatagtagta aacgcctggc tgaaaagaag
    19741 tcgtcttcaa taccgttacg ttatcttgca atgattcttt gtacgaagga tattttctaa
    19801 aagcggcatt caccgtaatc cattgcccat tcacatattc cgaagtaggc atcgtaacag
    19861 attggccatt atagcttcct ttaatcccaa atagattgta attaggggca gaagccaaag
    19921 tactgttccc ccagccactt tcaacgatgg cttgagcaat catgactgat gcatataagt
    19981 cattggcact tgccaaactt tgtgcagttg aggcaatact atcaatgaac gcttgttgag
    20041 aattagcaga tgagcgagca taaaaagcat agccagtata gttttcgaat tcagacaaat
    20101 ctgcttgatg ggtatgtgtc aatgtttgcc catctgtcaa ttggttcgtg atttcttctg
    20161 cattctctgg attgttctga gcttctgctg atgtactgtc ttcggtttgt gtttgttctg
    20221 ttgtttcagt tgttggttct tcggttgaaa ttgtattttc tgagacagta gctgactgca
    20281 aagtactttc tgtatcgttt tcaattcctg tttctgaaga actcgatgtc tgagttgcga
    20341 cagtttctgt cgattcttca gctagcactg ttgtgttggc aactgctaga tttcctaaaa
    20401 gaataacgct aaataataaa ttctttgctt tcatcttttt tctcctttga attatgaaac
    20461 ttttcctaaa taacagtgta actaaaaaag taaaagcaag tcaatgtcca tatttaaata
    20521 acatttgttt ctttttaata aatttgcttt ttctgcataa aaagacgtaa atgaagaata
    20581 ttcttctcca ttcacgcctt aaattgatgg tcgtttgaat aactatttaa caagtaattc
    20641 tctctggata ttatccgtta tggattatac ttcctgtgtc gtcggacgga taatcatgtt
    20701 gctgacagac atccggtctg gcgtttctat cgcaaatgct acagcagaag ctatatcttc
    20761 agatgttaat ccccattctt tttgcgcttc atgaagttca agagcagcct ttttgtcgct
    20821 gatggtttga tataattcag tttgaacagc tcctggagag atgatcgtag atttgatgtt
    20881 attttgccgt tgctcttgac gtaatccttc catgattgca cggacagcaa attttgttcc
    20941 gcaataaacg gcggaatcag gataaacaac atgtcccgcc acagaatcag ttgaaatgat
    21001 atgcccagat ttttgttttt ccatgattgg aagtacggcg gaaattccat ttaacacacc
    21061 catgatgttg atatcaagca tattttgcca ttcatcacgg tgaccttcaa ctaatggagc
    21121 ggttggcatg attcctgcat tattgtacaa tacatcgatt cgaccgaact tttccatcgc
    21181 taaatcgata actgcttgga cttgtgcaaa atctcttaca tctgctgttt gaatatatag
    21241 ctcagcttct ggtaaagatt cttttattgc ttttaggcga tcttctcggc gtgcagctat
    21301 aactagttta gccccttttt ctgctaatag cttcgttgtt gcttcaccga ttccgcttga
    21361 agcgcccatg atgacaatca ctttttctga taaatcattc attctattct cctcctgttt
    21421 tgaaaaatct cttgcttata ctcactatct cttatttcat cttactgttc aaataatccg
    21481 ttcgacaggt ttgacgattt tggcagggat tcctgcaacg atggtattgt ctgggacatc
    21541 tttcgtaaca gttgcattcg ctgcaacaat ggcattctcg ccaatcgtta cgcctggcag
    21601 aatagttgct cctgctccga tccaagcatt tttcttgatg tggattggct ttactgacaa
    21661 gcctcttcgt tcttttggat cggttagatg gttcaccgtg atgaggttga cacgaggacc
    21721 aatcaatact gaatcttcta aacagatgcc acctaagtca acgaacatca cttgacgatt
    21781 gataaagaca tttttcccta tactaatatg tttgccgaaa tctgtataaa aaggcagcag
    21841 aacagtcacc gtatcatcta ctttgtcact aatgatattt tctagccgtt tgcggatatc
    21901 tgctggttca tggtacccag tattcagcgc tgctaaaggc tcttcgtttt ccgcttggat
    21961 ctgatggatc acttcaaaga gcggatccgt tactcgaatc tctttattga cgatttgttt
    22021 ttcgatgagg cttgtcattt tgctccctcc ttttatcctt actttataac ggacggctta
    22081 tgatgtctaa tacttataaa acatacttaa ctattccttt taactatact tagtggtatg
    22141 cttttttaaa aacaggaggg aaaaaatgga tattcgtata ctgacgtatt ttttagcagt
    22201 agctagagaa aaaaatatca gccgagcagc aaaatctctg catatcacgc aacctactct
    22261 ctcaaaacaa ctgaaagatt tagaagaaga acttggtgtg acacttttca ctcgaggctc
    22321 ccgagagata cagttgacgg attctggtcg atatctttat tcaaaaggaa aagaaatctt
    22381 gtcgttggta gaaaaaacgg cgaacaattt aacaaatgac gcaatcatct ctggagaaat
    22441 ctacattggc ggaggcgaaa ctaaagcaat ctctttcatt tctgatagcc ttcattcgtt
    22501 gatcaaggcg catcctgata tccagtttca tttatatagc gggaatgcag atgatatcat
    22561 agacaaactg gatagcggat tacttgattt tggactcgta atcgaaccga caaacaaaca
    22621 aaaataccag tcatttcgac tgccagccca agatcgttgg ggacttttga tacatcggaa
    22681 tcatccttta gcaaagcaaa cagtcatcca tccgcaggaa ctgaaaactg ttcctttgat
    22741 catttcccgg caaacgtcag tggacagtca actagctgaa tggctaggtt tcagcataga
    22801 tcagttatcg atcgtcggaa catataactt attgtacaat gcttctttga tggtcgataa
    22861 cgggactttt ggtgcattag ctctagatgg gatcatcaat acttatggga cggaaaaagt
    22921 attcatccca ctcgatccgc cattacaagc ttcactgaat ctcatttgga aaaaagacca
    22981 gcctctttct cctgctgctc gtaaattttt agatgtccta acggagaaat acagttaagt
    23041 agagtttttt tccttcgctt tcagcttttt aacaacgaat aagttaagca aaagatccaa
    23101 tgattaaata acataagttt cttaatttgt tccttccttt tatttctgat aattccagta
    23161 aattactata attataaaaa aagaattgac aaaacataac tgtctatata aaattgatta
    23221 cgttaacaat aattcgtttc atgtgactag atcatactag gcatgaaaaa tctctgagga
    23281 acatggtttc cctaggaggt ttttcgtgtc tatttttgtt tggccgcaat caactggtct
    23341 agcaaacgtg aaaaccaaat cttcaaaaag ggtgcgcgca tgaaaatcaa aaagatcttt
    23401 aatcaaaatg cagtattggt agatgacggt ggacaggaaa aagtagctat tggaaaaggg
    23461 attggatttg acagaaaaag aaatgatttg atttttgatc gtgatgtcga acggatattt
    23521 gtcatggaac cagaaggtca aatcaagttg caaaatttac ttaatcagat tgatgaacgc
    23581 tttttatttg cagcagaaac gatcattgat catgcggaaa ctgttttgat ggaaaagttg
    23641 aatgaacatg tattgatcgc tttgaccgac cacattgctt tttctgcgga gaacattaat
    23701 aacggtatcg tcatccgcaa caaactttta agagaaatcg aagtgcttta cagtgatgaa
    23761 ttcagtattg ctcaatgggc agtagagtat ctgacaaaag agcttgggat tccatatact
    23821 tatgacgaag caggatatat cgcaatccat attcacagcg gacgaagcgg tcggaacaat
    23881 aaccatcgca gtatccgtga agtgacgatt gtatctgaca tcatccattt agtggaatca
    23941 gaattggaaa cagatatcca ttcagaacca ttttcactga attattcaag actggtgaat
    24001 catttacgac tgcttctaca acgaacccat gcgcaacaat atgctgtttt agacacggaa
    24061 atcgtagaaa tggtcaaacg aaaataccca gaaagctata agatttctaa aaaaatccgt
    24121 gtgttgctga ccaaagaata ccagctcgcg atcacgaaag aagaactggg ttacttggcc
    24181 atccatatcg agcgtttaag aagcgcaatc gtacaaacaa atgttaacaa tcatgaggag
    24241 gaaaaaaatt gaaagcatat ctacaacgaa tgggacgttc ccttcaactt ccagttgcgg
    24301 tcttacctgc tgctgcttta ctagtaggga tcggaaactg gtgggcatct tatagtaacg
    24361 acattgtcgc tcatttttta caagctggcg gtaacgcagt tttaggtcaa ttacctttgc
    24421 tgtttgccgt tgggttagca ctagggatgt cagtggacaa atcaggtgct gctgccttgg
    24481 caggtttggt cgcatttgaa gttccagtaa atgttttaaa aactgattca gtagctactt
    24541 tattgaatat caaaccagaa tcagttgatc catcttttgc tcagatcagt aacgtattta
    24601 ttggtattat tgccggattg attgcggcag ctctttataa tcgtttccac gaagcaaaat
    24661 taccgatggc tttatctttc tttagcggca agcgtctagt gccaatcttg gctgcctttg
    24721 tcatgttgct gatcagtgca gtgctactgc tcgtatggcc tccggtttac tctgctttag
    24781 tttcttttgc taaattcatt ttgagtttag gctggggtgg tgcaggtctt tatggcttct
    24841 tcaaccgttt actgatccct acaggattgc atcaagcaat gaactccgtg ttctggtttg
    24901 acgtagcagg aatcaatgat attggtaaat ttttggcttc tgaaggaaca aaaggcgtaa
    24961 caggaatgta tcaagctgga tttttcccaa tcatgatgtt cgggcttcca gcaggagcat
    25021 atgctattta cagaaatgca cgtccagaaa acaaagcaaa aacagcttcc ttgatgttag
    25081 cagcagcttt tgcttcattc tttactggtg taacagaacc acttgaattc tcatttatgt
    25141 tcgttgcttg gccattatat gtcttgcatg caattttcac tgggatttct ttagcagtga
    25201 gtgcattctt ccattggaca gcaggatttg cctttagtgc cggatttgtc gattacttcc
    25261 tatctttgaa aaatccgatt gcgaatcagc cattgatgtt gatcgtccaa ggattagtaa
    25321 cagcagtgat ttactacttt ggctttaact ttgctatcaa aaaattccat ctgatgactc
    25381 ctggccgcga ggaagcagat ctttcagatg acgatactgc aacgactaat tcagacaata
    25441 aatatgcggc acaagcaagc aaaatctacg ctgcattagg cggagccgac aatgtaactt
    25501 cgatcgataa ctgtactaca cgccttcgtt tgcaagtcaa agatacaggc ctgatcgatc
    25561 aaaataaaat caaagccact ggcgtacctg gtatgaagat catcgacgga aaaaatgcgc
    25621 aagtcatcgt aggtacagaa gtacaattcg ttgcagatga gatggcaaaa ctacatggtg
    25681 gtgctgcaac acgacctgca caaacaaaca ccgtggttaa aacagaaaca tttgccggtg
    25741 gaacgatcga acaagatatt tacgctatcg caaatgggaa actgattcca atcactgaag
    25801 tttcagatga tgtattcgcg gaaaaaatga tgggtgacgg ctatgctgta ttaccagaaa
    25861 atggagaaat cttctctcca atcgctggta cgatcacaaa cattttccca acgaaacatg
    25921 ctgtcggaat ccaaacagat gctggtatcg aagtcttgct tcatatgggc atcaacactg
    25981 ttgacttaaa aggcgaacca ttcactttat atgtaaaaga aggtcaaaaa gtcgcaagag
    26041 gtcaattgat tgctttagtt gatcttgccg ctattcaatc tgcaggcaaa aacacggata
    26101 tggtcgttgt attcacaaat ggcgacaaag tccaaagctt agaaatcgaa cccgcacgtg
    26161 atgtaaaagc aaatgataaa atcggctctg tttccaacaa agcctgagcc atcgtcttcc
    26221 ccagactttt ttatcgataa acaaacagga gctgagacaa aaatcttgtc tcagctcctt
    26281 tttccgaata aaaacggcgt tctcctgcca actccgcggt aataagtcca gccaaacgta
    26341 acatatgaag agctattttt tatttgactc ttcttattac ttgatgcaga acagcttttt
    26401 tacaacctct gataaacggt gttcaaaaac cagtttatcg gtatgaagtc aaatttatgc
    26461 aaaaatgtgg aaagccattt tcacaaaatt tttcttcata ctcaaaacta atcggctttt
    26521 tcacaacctc tttatacatt attcctgatc caatatttct tttaatgctg cttcaagaga
    26581 agttaatggt tttcccaata ctttttctaa atcatcagag gaaatagcta actgatcctt
    26641 tttaatatca tgttggatcg ctaaaaacat gtcagctgct tcttgaggga atccggcttc
    26701 ttttagattt tcgacaaacg ctgcatcact tgcttcaata atttctaact ttttacctgt
    26761 cgcacgctct aatgccttgg ctaattcttc atagtcagtt agattgccgg ataattctaa
    26821 gatttctgga gagtcacttt ctactagtac ttttgctgca gcttctgcat attcacgttt
    26881 caatgcccaa cctgtttttc catttcctgc agcataaacg aattgaccag tttttaaagc
    26941 ctgcccaatg ataggtaatt cattctctac ataccaatta ttccgtaaaa atgtatggga
    27001 aatacctgaa tccttaatca ttttttcagt gaattggtga tctggcgcta atacactgat
    27061 cgagtgatcg gcatctgcaa aacttgtata agcgatataa gataccccag ctgcttgtgc
    27121 tgccttcact acattcttat gttcggcttg acgatttcct ggaacgccag agacaaaaag
    27181 caatcgatct attcctttca acgcttcttt cattgagtct aaatcatcat aatcaccaat
    27241 acgtatatta aatccagctt ctttcagtgc ttctcctttt tcttcactac gtactagtac
    27301 atagatttca ttttgtggaa ccatcttttt taagaattcc agcgcataac caccaaaacc
    27361 tccagttgct cctgttacta aataatgcat atttgccact ccctattctt tttatttgtt
    27421 aagatttaca gctgtacatt tataaattac acctttatca taaagagtgt ggtaagataa
    27481 gtcaagcaaa aaaaatgaaa ggatgacaac tgatgaagta ttctctgcaa tttagtgatg
    27541 caatccatgt acttgcctat attgaaatgt ttcaagatac ccgatcactt tctagtgaga
    27601 tgattgccag tagtatcgaa acaaatgcag caaatgtacg taaaatcatg agtcagctca
    27661 aaaaatccgg tttgatcttt acaaaaaccg gaaaaccgca gccttccctt gccaaaagtc
    27721 cagatcagat ttctttacta gatgtctatc gcagcatcga aggtaataca cagcttatcc
    27781 aagtcgatcc aaaaacaaat ccaaattgcg tcgttggtgc gaatatccaa gaagctttag
    27841 aagaaagcta cgctcggctg cagaaaaagg cagaagaaga aatggctaag atcacacttg
    27901 atagtctgat acataaaata gccgttttgg aaaaagaaaa aagaccagaa aacacaggcc
    27961 tcgtagagag atttttataa ttctctctac tgtgcctgca tttttctgat cggaatcgtc
    28021 tacataaatt cttttattct ccgggggaca aaagccttgt catgcccctt tttcctctct
    28081 aatgctcagg tcaaagcgct gaagtcatga cgtcttgata aacggtgttc aagtagctga
    28141 ccctcggcat tagttctgaa aaatgcaaaa tatggaaagc tatttcccat ttttcctctc
    28201 taatgctcag gtcaaagcgc tactttcacg acctctatat tatcatggtt tattgactac
    28261 cggtatccag atttctccca tatatttccc gttggtaact cccatttcga ctacagcatt
    28321 tggaccccca acataggcgt aatcttctac ttctcttaaa acatgcccaa aggccgtatt
    28381 cgtcaaatca tttcttaatt cttcaggcga ttctgctaaa gcgattacaa caagatacgt
    28441 tccttttggg aatgggatga ttcgggtagt gtttggaaca tcctgactcg tttgaacacc
    28501 gacatagtac atcattttcc cgttataggg ttcgttgact gtaaagaaat aatgattttc
    28561 agcagcttgt ttcaatgcat ctattgttcc attaccgata actttttgct caaatgcttg
    28621 tttctctgca gccaaaccga caaaatccgt attgctactc ttcaattcca caccaatacc
    28681 cgtcaccgta aaatctggtt tttcttcgat cctatacttt gccatttctc ccaccctttc
    28741 tattatatta agttcatctt acataaaatt aaagggaaat gacacgatta cgctcttttt
    28801 tctagggata tttttatttt ttaactattt ttttgctttc actgcaatcc aaatctcact
    28861 gtaaacatta ctgaaatcct ctccccattt tgtaaaagag atctcgggag cttcgactaa
    28921 ctcataatct gaagaaggaa gccattctgc atagattttc ccccaagtat cttgtaaagt
    28981 ttggggaaat ggccctttat taggaaaaat ggcccaagtg tgttcttcta tagcaagcat
    29041 ttctaaatca gcgtacggat tttcttttgt cgtagcaaag ccaatcatgt gcatcaaact
    29101 gcccttttct tccaagcggg ctccatcaaa tccataagaa acgttgatta cttgatttgg
    29161 atataaatct cctaacttat gcatctcttc cctttgagtc tccgtgatcg tctgcgctaa
    29221 cttttgtatt tcttgattga ctccttcaaa tttgacagga acttttttcg ccacacctgc
    29281 cacataaaac tgttctttct tttcgatacg aaattccatc gattttcctc ctctgacatc
    29341 aataaaaaat gtcaagcgtg gaaaagattt ttgtatgccg tatctgctga tttctgaagg
    29401 cagatatcct gaccaatcac gaaaagctct tgaaaaacct tcgactgttt catatccata
    29461 tctaaacgcc gtttctgtca ctcttgatcc atctaggaga tcgcgattag ccaaagccaa
    29521 tctacggttt ttgatatagt ccattagcga catccctgca ataaacgtaa agatgcgttt
    29581 taattgatat tcagagattc ctgtattttc ggctattatt ttataggaaa gtccttctgt
    29641 caaatgttct tcgatgtaat cgatcaattg gttcagatct ttaagcatat ttatcttcct
    29701 ccttgcttgc ctttttatca tattatcaaa gaaagtcaat tacttgatga atcaggaacc
    29761 aatttagtca gatgtagaaa atgttgtatg gatctaaaga aaattctcaa gcagcgtttc
    29821 gtaacggata attggtatta tctgcctatc tatgttttac ctttagaaaa ataaattttg
    29881 gggaagacac attctaagta aacaaagcgg taaaaatatc ttttattccc ttagttaatc
    29941 cgcttactac ctactgtaca aagcaaattg ttgttcaaac tgctgatcag aacgctgctt
    30001 ggatttttct ttgcgataca tcatacctct tttatcaatt ggaacaatta tttatttcta
    30061 acaaaatcac ttcatttttg tcagataaat aaaa
//
LOCUS       DARBDV010000026        27979 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000026 DARBDV010000000
VERSION     DARBDV010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.026"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            8..649
                     /locus_tag="SCM56_001106"
     CDS             8..649
                     /locus_tag="SCM56_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109922.1"
                     /translation="MIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGEIYQAEGLSAV
                     LHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTAAELQKQMRYQ
                     IDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITMIH
                     RFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            708..1148
                     /locus_tag="SCM56_001107"
     CDS             708..1148
                     /locus_tag="SCM56_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1109923.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1160..1828
                     /locus_tag="SCM56_001108"
     CDS             1160..1828
                     /locus_tag="SCM56_001108"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEG1109924.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1892..2515
                     /locus_tag="SCM56_001109"
     CDS             1892..2515
                     /locus_tag="SCM56_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEG1109925.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2571..2957
                     /locus_tag="SCM56_001110"
     CDS             2571..2957
                     /locus_tag="SCM56_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEG1109926.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3170..4561
                     /locus_tag="SCM56_001111"
     CDS             3170..4561
                     /locus_tag="SCM56_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HEG1109927.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            5172..5540
                     /locus_tag="SCM56_001112"
     CDS             5172..5540
                     /locus_tag="SCM56_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEG1109928.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5720..6661
                     /locus_tag="SCM56_001113"
     CDS             5720..6661
                     /locus_tag="SCM56_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEG1109929.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6730..7461
                     /locus_tag="SCM56_001114"
     CDS             6730..7461
                     /locus_tag="SCM56_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEG1109930.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7605..9785
                     /locus_tag="SCM56_001115"
     CDS             7605..9785
                     /locus_tag="SCM56_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEG1109931.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9805..10287
                     /locus_tag="SCM56_001116"
     CDS             9805..10287
                     /locus_tag="SCM56_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEG1109932.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            10303..11064
                     /locus_tag="SCM56_001117"
     CDS             10303..11064
                     /locus_tag="SCM56_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEG1109933.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            11195..12529
                     /locus_tag="SCM56_001118"
     CDS             11195..12529
                     /locus_tag="SCM56_001118"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEG1109934.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12836..13321
                     /locus_tag="SCM56_001119"
     CDS             12836..13321
                     /locus_tag="SCM56_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HEG1109935.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            13314..13997
                     /locus_tag="SCM56_001120"
     CDS             13314..13997
                     /locus_tag="SCM56_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEG1109936.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGIILAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            14123..16768
                     /gene="polA"
                     /locus_tag="SCM56_001121"
     CDS             14123..16768
                     /gene="polA"
                     /locus_tag="SCM56_001121"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEG1109937.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16813..17649
                     /gene="mutM"
                     /locus_tag="SCM56_001122"
     CDS             16813..17649
                     /gene="mutM"
                     /locus_tag="SCM56_001122"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEG1109938.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17613..18242
                     /locus_tag="SCM56_001123"
     CDS             17613..18242
                     /locus_tag="SCM56_001123"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEG1109939.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            18294..18626
                     /locus_tag="SCM56_001124"
     CDS             18294..18626
                     /locus_tag="SCM56_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEG1109940.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18938..19429
                     /gene="nrdR"
                     /locus_tag="SCM56_001125"
     CDS             18938..19429
                     /gene="nrdR"
                     /locus_tag="SCM56_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEG1109941.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19452..20825
                     /locus_tag="SCM56_001126"
     CDS             19452..20825
                     /locus_tag="SCM56_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEG1109942.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20825..21751
                     /gene="dnaI"
                     /locus_tag="SCM56_001127"
     CDS             20825..21751
                     /gene="dnaI"
                     /locus_tag="SCM56_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEG1109943.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21880..22320
                     /locus_tag="SCM56_001128"
     CDS             21880..22320
                     /locus_tag="SCM56_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEG1109944.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            22464..23165
                     /locus_tag="SCM56_001129"
     CDS             22464..23165
                     /locus_tag="SCM56_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEG1109945.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            23162..24283
                     /locus_tag="SCM56_001130"
     CDS             23162..24283
                     /locus_tag="SCM56_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEG1109946.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            24298..25530
                     /gene="pepT"
                     /locus_tag="SCM56_001131"
     CDS             24298..25530
                     /gene="pepT"
                     /locus_tag="SCM56_001131"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEG1109947.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            25662..26570
                     /locus_tag="SCM56_001132"
     CDS             25662..26570
                     /locus_tag="SCM56_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HEG1109948.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            26605..26877
                     /locus_tag="SCM56_001133"
     CDS             26605..26877
                     /locus_tag="SCM56_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEG1109949.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            26888..27934
                     /locus_tag="SCM56_001134"
     CDS             26888..27934
                     /locus_tag="SCM56_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HEG1109950.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         9403 a         4843 c         6097 g         7636 t
ORIGIN      
        1 agatactctg attgagcgat ctacatttct atatgttcaa ttgcaggaaa ataaaacatt
       61 gctgcatttt tcaccagagt tccatctaga ggggctaaca ttaggagaaa tctatcaagc
      121 agaagggctg tcggctgttt tgcacttttt taaggtagaa ttggaattac cagtaaaaga
      181 ttatatcgag ctttctcttg aatcttggga tcgagcagtg gttgaactgt ttccagaagg
      241 tttaatgatc gatacaaacg atgggaagat ccatctgact gcagcagaac tgcaaaaaca
      301 aatgcgttat caaatagatg acgaaaattc tttttctatt tttagacgtc agcaaaagat
      361 tctaaaaagc tttttaaaag tgatcagtaa aaaacggaat ctgatcaaaa cgacacagct
      421 gttgaaaaaa tatccaggtc tgcttcacac gtctattcaa tttcctcaat tgattacgat
      481 gatccatcgt tttatacgta tgcaacagct accatctaag aagctgtttc ttccattgac
      541 ggacacattt cgagtagtca acagagaaga taaaaagaaa gtgattgtga ttgactttac
      601 cagaaatcgg aatgtccttc accaagttgc aatggaaaag gccaattaag gctttttctt
      661 tttggatttt tttgtcataa taaatattag atgatggagg agataatatg atgcaaacat
      721 ggttagaaag gctaatacga tttcagcaga actatcacaa acttatgcag ggaagatatg
      781 cgaaatttga tcagttgaat cgcggtctat tgatcgctgc gcttgtcttt agtttgctaa
      841 gtcgttggtt gccctataga atcggacaat tgttgttcat cgtctttttc ggatggatga
      901 tatttcgttt cctatcaaaa aaaatctacc ccagactaaa tgagaatcaa cgttatttga
      961 aatatcttga tagagtcaaa gaaaaatatc atcagataaa aaacaaaaag caagtcaaaa
     1021 tggatcgaaa aagttatact ttttttgagt gcccgaattg tcatcaaaag cagcgtgcac
     1081 ctaaaggaaa aggacgaata cgtgtgacat gcaaaacatg tggtataaaa ttcgaaacga
     1141 atgtttaagg gagaaaagaa tgaaattagt attattacgt catggcgaaa gcgaagcaaa
     1201 ttttgaaaat tactggacag gatggctaga tgtcgcctta acagaaaaag gaaaagaaca
     1261 agcagcagtt gccggtcaaa aaatgcttga agccggactt caattcgatg ccgtttatac
     1321 ctctgttttg aaacgtgcga ttcttaccgg acaagtcgca ctagaagaaa tgggtcagtt
     1381 atggcttcct ataataaaaa catggcgtct aaatgaacgg cattatgggg atcttgtggg
     1441 taaaaacaaa gataagatga aagaaatata cggaaaagat caagtaaaaa catggcgcag
     1501 aggatatacg gaagttccgc cgttaaccgc agaaaataga tttgaccgca gatatgatca
     1561 cttggatccg catttgatcc catacggaga aagtctggaa atgacagtga atcggatcat
     1621 tcccttatgg caagatcatc tagcaccaaa actaaaagat cggcaagatt tactagttgt
     1681 gggtcatggt aatagcatcc gagcactgac aaaatattta gaagatgttc ctgaagatca
     1741 aatggatacg atcgatatcc caaatgcaca accaatccag tatacattag cagatgattt
     1801 gacaattctg gacaaaaaaa tactttaaag aacaccttta tctaaaagtg agagatagaa
     1861 aaaagaagca aaggaatgga acaaaaggaa catgaaaaat tatatatggg actttgatgg
     1921 tacattgttt gatacgtatc ccgcaatggt agatggtgca tggcaagcac taaaagattt
     1981 tggtatcagt atggacaaaa aagaaatcta cttcaaaatg aaaaagtatt ctactagtta
     2041 tttgatcaat gaatcaaact tgaatgccag agagtttaat gaattatttc accgatatga
     2101 aaaagaaagt acggaagttt cccgtccatt tccagaaacg aaacaagtac tggaaatgct
     2161 aaaagacaac ggcggtcgtc atttcatcct gacccaccgt ttgacagaat caacatgggg
     2221 cctgttaaaa gaacatcgat tggcacatct gattgaggaa gtagtaggga tcgatcagga
     2281 ctttcctcgt aaacctgatc cagcatcttt aaattatttg atcgatactt ttcatttaga
     2341 aagaacagac acgatgatga tcggcgatcg tcgtttagat atcgaagcag ggaaaaatgc
     2401 aggagtcgct acttgtcttt acgacatcga tcattttctg ggtgagatcc cggcagatta
     2461 tgtggtagga aatctgaatg aaatccttac cttgcagagc atcattaaaa agtagtatcc
     2521 cctttcaaag gagatatgtt ataattttca taaaggagtg ataaaattat atggaaatca
     2581 gaagacaaaa agaagttgta gaagcatttc attatgatat gagattgccg aaccaagaag
     2641 tagaaacaga tttgaaagta ggcttctcgc ctttaaaaga aacgatcgaa aactatccga
     2701 aagaacatag catcgtagga gcacgcttag agttccgttt agtgtttgat acatatgttt
     2761 tatctggaag tgtgagtcag atcaatcata tcctcaatcg gaaaattgaa ggtcaaaatg
     2821 atgtcacaca agaagaagtg gacgagctag tagcaccgtt attcgatatc gtacgccgaa
     2881 tgtcttatga ggtgacagag atcgccttag atcagcctgg ggtaaaattg aattttcaat
     2941 cagaaaaaca agaatagaca gtaaaaacat acctgcgata gattaaagct atcacaggta
     3001 tgttttttta ttactaattg taagcaaaaa gaatgtcatt tcgataaaaa tcgtctaaaa
     3061 tataaaagtc actgaaaaaa attttttcag gaggatggtt gaagaatcat tctcgggaac
     3121 ctttacttcc ttcgatggac ttgtcattaa taatatgagg aggagaaaaa tgacaacact
     3181 catcacagtt ttagtggttc tagtttttgc ggcattatta tatgtgttct atcgtatgca
     3241 aaagcgccat acaaaatttt caacacgcgt ctttacagca ttaggagttg gtatcgtctt
     3301 tggcggtctg cttcaattga tctttggtgt agacgacaaa gtcgttactc aatcaatgga
     3361 atggatcggg attgtaggtc aaggatatgt atcattcttg caaatgctag ttatgccttt
     3421 agtgtttatt tcgatcattg gtgcattcac aaaaatgaaa gtttctgaaa agctagggaa
     3481 aattagtgcg actgtattga cttctttact tggaacaaca gctattgcag cattgatcgg
     3541 aattggttca acaatgctgt tcggactcca aggagctaaa ttcacacaag gagctgcaga
     3601 aacagcacga atctctgaac tggcaacacg tcaggaaaca gttgaaaact tatcgattcc
     3661 acagcagatt ttatccttta tccctaaaaa cgtttttgct gatttagcag gtagtcgtgc
     3721 aacaagtacg attggagtcg ttattttcgc ggcatttgtc ggaatcgctt atctaggcgt
     3781 gaaaagaaaa gcgccaaaag aagcagcatt cttttcagat ttagtcgaca gtctgtataa
     3841 aatcattatg cggatcgtga cgcttgtttt gcgtttgaca ccttatggag tatttgcttt
     3901 gatgacaaac gttttggcaa caagtgattt tgcagctatc gtcaatttag gtaaatttgt
     3961 cgttgcttct tacgtagcat tgcttgctgt tttgattgtc cactcactga ttttgattgg
     4021 tgcaaaagtc aatccaatca actatttcaa aaaagccttt ccggtattga gctttgcgtt
     4081 tacttcacgt tcaagtgctg gagctttgcc attgaatatc gagactcaga caaaagcttt
     4141 aggagtagat gatgcaacag ctaatttctc agcaagtttt ggtttgtcta ttggacaaaa
     4201 cggctgtgct ggcgtttatc cagctatgtt ggcaacgatc gtcgcaccag cagctggagt
     4261 gaatgtcttt agtttggaat ttattttgat gcttgtggca attgtaacaa tcagttcttt
     4321 tggtgtagct ggagtcggtg ggggcgcaac atttgcttca ctgatcgttt taggggcaat
     4381 gaacttgcca gtagcaatcg ttgggttagt tatctctgtt gaaccattaa tcgatatggc
     4441 acgtacagca gtaaacgtca gcggtagcat ggtagctggt atcgtgacaa gtgcacgcat
     4501 caaagattta gatcgcgatg tcatcaatga tgattctaaa gtgatcgatg tgaatgcata
     4561 gtcaaaaaga ggttgtgata gaagtggtca gctttaagaa ataagaagaa gtttcctaaa
     4621 aatagtttct caaatttttg tgaaatttcg gcttatttcc gagaagctac ttctgaaaca
     4681 ccgtgaataa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
     4741 aaatagttcc tcatatttta cgtttggttg agcttattac cgcagatgca agcttttgaa
     4801 caccgtttaa gaaagaggtc gtgagcctgc cgtttcactc tgagtcacaa agagcaattt
     4861 caaaaaatag cttctcatgt ttgttgaata ttgtgtcttg tgaccgagga gttgtcagga
     4921 gaacaccgtt taccagaggt tgtgacttgt ttttagtcac gacctctttt tttatttatt
     4981 ccatataaat acacattatt tattccgaaa agtgaaagcg caaataacaa aaacaaccaa
     5041 cttatttatt attaaagaaa agcgtattta agtaggacga ttctgttgag agctttcgta
     5101 gattttccca ttttcctatg gtaaactgac aaagttgaga atgttagaac agatcgttta
     5161 ggaggaaaca gatgtttacg aatgaaataa aaatcatgct ttatgtggac gatgtagaaa
     5221 aaaatgcaac gttttggaga gcagttggct ttgcggaaaa agatcgtcaa gaaatggatg
     5281 ggacgcttat aattgaagta ggagttgagg acagtcaaac ctcatttctg ttatatgatc
     5341 gagcatttgt tgagtcccat tcacctggca cagctactag tgccccatct ttgatgtttt
     5401 acagtaaaga tatctttgga ctatataaaa aaatgcagac gcaatcagtc acattaggtg
     5461 atatggtaca gcttggagaa gaatatgtct ttaactttgc tgatcccgat ggcaactact
     5521 ttgcggttac aggtaaataa tagaaataaa gactttgttt ttctgtgcga aacaggaaag
     5581 cgaagtcttt ttaattcaaa taataatatt atgtaaacaa aaaactttcc atgcgtaatt
     5641 atacacaagc aaataaatcg tcaaatgcga acgccttctt tatactgttt aggaacgaaa
     5701 agaaggaagg aagaaataca tggattatca agtactgctt tattacaatt atacaaaaat
     5761 cgaagaacca gaggaatttg ccaaaaatca tttagccttt tgcaaatctt tggatcttaa
     5821 aggtcgtatt ctagtagctg aagaaggaat aaatggtact gtgtctggaa ccgttgaaaa
     5881 cacgactgcc tatatgaaag caatgaaagc tgatcctcga tttgctgata cgatgttcaa
     5941 gatcgatcca agcgaaggac atgcctttaa gaagatgttt gtaagatacc gtccagagct
     6001 tgtaagttta aagctagaag aggacattga tccaaaccaa gtgacaggtg cctatctttc
     6061 accgcaagaa tttaaagaag ctattttaga tgaacagaca gtggtgattg atgcacggaa
     6121 tgattatgaa tatgacttgg gacattttcg aggggctgta cgcccagata tacgcagttt
     6181 tcgagagctt ccagactgga tccgcgagca caaagaagaa tttatggaaa aacgggtagt
     6241 gacctactgt actggtggca ttcgttgcga gaagttttct ggttggcttg tccgtgaagg
     6301 attcaaagat gtcggacagc tgcatggcgg tattgctact tatggtaagg atcctgaagt
     6361 ccaaggagaa ttatgggacg gcaagatgta tgtgtttgat gaacggatcg ctgtcgatat
     6421 caaccaagta gataaacaaa tcgtcggtcg cgactggttt gacggaacac catgtgaacg
     6481 atatatcaat tgtagcaatc ctgagtgcaa tcgtcagatt cttgcttcca ttgaaaatga
     6541 agaaaaacat ttaggagcat gtagcataga gtgtgcgaaa catccgcgca atcgctatgt
     6601 tcaagaccat ggaatctctc cagaagaagc agaactgcgg tttacagctt taattaaata
     6661 gaaaacaata gttttgattg tcgaccattt ccaatatgcg ctatactagc gttgaggatg
     6721 gtgatccaca tgaaatcatt gatttatgct attggggatg tccatggcaa acatgagctt
     6781 tttgaaaaac ttattcacta ttatgatcca aagatccatc agcttgtttt attaggggat
     6841 ctcaatgatc gaggtcctcg tacaaaagaa tgttttttat taggaaagca attagtcgaa
     6901 gaaacaggag ccatttacct ccggggaaac catgaagaat attttttgca atttctgcaa
     6961 tcaccggagg actggtatgc gccttatatg agaaatggtg gaaaagaaac gtttgaaagc
     7021 ctgattcacc caggggcatc ggaggaatac tccccaactg aaatctcgat gatgatccaa
     7081 tcgagatatt cagaactgat taaattttta gtccagcgcc ccttgtatta tgaatgggaa
     7141 aattatcttt ttgtccatgc cggtgtggat ttgacaaaga aagactggcg taagacaagt
     7201 ctaagagact ttatttggat aagggaacca tttcatgaag ggaaaaacaa tacaggtaag
     7261 acaattgtat ttggacatac gattacacca atgctccatg gagatatgca gacaacagac
     7321 ctctggattc aggatcataa aatcggaatg gacggtgggg ctgtattcgg agggtcattg
     7381 catggggttg tttttgacaa ggaaggtatt cttcaagata ttgaatatca gaacaacgaa
     7441 ggaccttggc atggaatctg actaatggaa agaatgaagg agctgtgact ttgttgcggc
     7501 tctttttatt tttatagaga atattgtttc tttcagtagt ttaacccagg ataaaaagta
     7561 tgatagaata aagaagcaat ttttagcaga aaagaggcat tttaatgacg gaaaatttga
     7621 accagacgat cattcagctt gtacaaaaag aactttcaga ctatcgtcct aagcagttga
     7681 ctactgtact taatttgtta aatgaaggaa acactgttcc ttttatcgcg cgttaccgaa
     7741 aagaaatgac tggaagtctt gatgaagtcc agattcgtga aatcgaagag cggtatgcct
     7801 atcttgaaaa cttagaaaaa cgtaaaactg aagtgattcg gctaatcgat gaacaaggga
     7861 aactgactcc cgagttagaa gcagaaatca cccaagccgt gaaaatgcaa caagtggaag
     7921 atttgtatcg tccatacaaa caaaaacgac ggacaaaagc aacgattgct aaagaaaaag
     7981 gacttgaacc actcgcgaag tggctgatgc agcttacaga tggagaggtg caatctgaag
     8041 cagaaaaata tattgacaaa gaaaaagaag tctcttctgc cgaagaagcg ttgcacggtg
     8101 ctcatgagat tattgctgaa caagtcagcg ataatgcaaa atttcggact tggattcggt
     8161 cttatacgta taacaaaggt atgtatgtca gcaatgtcaa ggatgagcaa gcagatgaaa
     8221 aaggcgtata tgaaatgtat tatgactttg cagaacccgt ccataaaatg gtttctcatc
     8281 gaatattagc tactaatcgt ggagaaaaag aagatattct gaaagtattc tttcaagtag
     8341 atgaagcagc aattttagct tacttggatc gccaaatagt caaaaatcct gcctctcctt
     8401 cttcttcatt cgtcagagaa gcctatcagg atagttataa acgctttatt cagccagcaa
     8461 tcgagcgaga actgcgcaat gaattgactg aaaaggcaga tgagcaagca atcgctattt
     8521 tcggcgaaaa cctccgtaat cttttgttgc aaccgccact aaaaggtaaa gttgtcctgg
     8581 gatttgatcc ggcttatcgt actggatgta aactagcagt cgtagatgcg acagggaaag
     8641 ttttagcaat cgaagttatt tatcctcata aacctgcagc gcaagcaaaa agagaagctg
     8701 ctggaccagc ttttatccaa ttaattaata aatatcaagt agatatggta gctatcggaa
     8761 atggtacagc cagtcgggaa tctgaacttt ttgtggctga gcagttgaaa tccgccgacc
     8821 ataaaaccta ttacgctatc gtcaacgaag caggcgcttc tgtttactcc gctagcgaaa
     8881 ttgcacgaaa agaatttccg catctacaag ttgaagaaag aagtgctgtt tctattgccc
     8941 gtcgcctgca agatccttta gctgaattag tcaaaatcga tcctaaagct gtcggagtag
     9001 gccagtacca acatgacgtt tctcaaaaac gtttggctga acaattggat tttgttgtgg
     9061 aaacagcggt taaccaagta ggtgtggatg tgaatacagc aagcccgcag ctcttgcagc
     9121 atatatcagg attgaataaa acaactgccc aaaacattgt catttatcgt gaagaaaacg
     9181 gagaattcac cgcacgaact caattgaaaa aagtgccacg cttaggacca aaagcatatg
     9241 aacaagcaat cggattcttg cgagtccctg gtgggaaaaa cattttggac aacacaggta
     9301 tccatcccga aagttattct attgcaaaag aaatattaac ttcagtccaa ctttcagaaa
     9361 aagagcttgg tacagaagaa gcagtagaaa aattgacacg attatccgta gagaaactgg
     9421 ctgaaagcct atcagtcgga gaagaaacgt tagccgatat tttagctggg ctgacacaac
     9481 ctggacgaga tatgcgtgat gagatgccag cgcccttatt acgtacagat gtattgagta
     9541 tggaagactt gaaaccaggg atggaattga ctggaacggt ccgtaatgtc attgacttcg
     9601 gcgcatttgt tgatatcggt gtgaagcaag acggacttgt ccatatatcg aaactcagca
     9661 aaaaattcgt caagcatcct acagatgtcg tgtcagtagg agatatcgta acagtttgga
     9721 tagaacaagt cgatacgaaa aaaggcagaa tcagtttgac gatgctttcc ccttatgaag
     9781 agtaacaatg aggagtaaca aaaattgaca caagaagaac tgcagcagtt agtagaagag
     9841 atatcattaa aaagcttcca gcttcctttc aagcaccaag caatgttcaa tccacgctta
     9901 cgtacaactg gcgggcgcta tcatttaaac gatcatcatt tagattttaa tgcgaagcta
     9961 tttgaagagt cagacgaaga gacgattgca ggaatcatca aacatgaact ttgccattat
    10021 catcttcatc tagctggaag agggtatcag cataaagata tggatttcaa gtatcttttg
    10081 aaaaaaacag gagggttgcg ctatgcacca gccttgacga agaaacagat aaagatggag
    10141 tattatcgat gccaagattg ttctgccacg atctgccgca agagaagaat cgatgtacgc
    10201 aaatatcgtt gtggaagatg tcatggagaa atcaaatgga ttgagacaaa aagagaagac
    10261 tttaatgaag cacaaaaaat cagatgaaag gatagaaggc ctttggaaaa aagtgagcag
    10321 aggaatctaa aagactggaa tcagctatta aattttttag ccgcacctga agagggaaat
    10381 tttgaggaac tgacggcacc tacacttatt ttagctggaa actgtcttcc tatcttagct
    10441 gataaagcag cacaaatgta tttaaatggc caagtagatc aattgttttt ggtcggaggg
    10501 gtggggcatg cgactagaat cctttatgag aattttgaaa agcaaggatt tcattttgaa
    10561 gatggcatga gtgaaagtga aatctgtcgt cagtatctaa aagaagtcta tgatttgccc
    10621 gataaggctt ttctgataga atcgaagtct acaaattctg gagagaacgc cactttttca
    10681 ctggagattc ttcattcctt aggagctgta ccagaaaaaa tcttattgat gaatgatccg
    10741 acactgcaac gcaggactcg agcaactttt gagaaagtat ggcaaaatga acaaactatt
    10801 ttcgcgaatg ctgtgccatt tgtgccggaa atccttcact tttctgaaga gatcatcttt
    10861 acagcaaaag aactgaatca tcaatggcct aaggaatatt tccatgcgct agtattagga
    10921 gaaatggaac gtctccatga cgatgagaac ggctatggtc cgaaaggaaa agattttatt
    10981 cctcacattg atatatcaga ggaagtttgg tcttcttaca ccagaattaa agagtcaata
    11041 aaaacggatt tttctcgtac ttgaccattt tagcaagcga atcctccttt ccaaataaat
    11101 ttggttgaaa ttttcaatcg tttataagat aatgaggagc gtgttgaatg taatagctaa
    11161 aagaatggac gctagataag agaagaggat tcaaatggaa aatcaaaata aattgtatca
    11221 ttttgttggt atcaaaggtt caggaatgag ctccttggct cttgttttac atgaacaagg
    11281 tctaaatgtc caaggatcag acattgaaaa atatttcttt acacaaagag atttagaaaa
    11341 agcaaatatc acgattctgc catttaacgc agataatgta aaaccaggga tgacgatcat
    11401 tgcaggaaat gcatttcctg attcacatga agaaatccag cgagcaaaag aattaggttt
    11461 ggaagtgatt cgctatcacg actttattgg tcattttatc caaaattata cgagtatcgc
    11521 tgtaacaggc tctcacggaa aaacaagtac gactggactg ctctctcatg tattgagtgg
    11581 tgtccgtcca acaagttatc taattggaga tggaacagga catggtgatc cgcaagcgga
    11641 attcttttca tttgaggcct gtgaatatcg ccgtcatttc cttgcctatt caccagacta
    11701 tgcaatcatg accaatatcg attttgatca tccggattat tatacaagta tcgacgatgt
    11761 atttacagct ttccaaacaa tggctggaca agtgaaaaaa gctatttttg cgtatggtga
    11821 cgatgcttat ttacgcaaat taaaagcaaa tgtgccgatt tattattatg gtgtcacaga
    11881 aaacgatgat atccaagcac gaaacatcga acgcacaact tctggttctg cttttgatgt
    11941 ctatcatggg gatgaatttg ttgggcactt cactgtacca gcattcggta aacacaatat
    12001 cttaaatgct ttaggtgtta ttgcagtagc ttatttcgaa aaactggatc tgaaagaagt
    12061 tgcagaagaa atgctgactt tcccaggagt aaaacgtcga ttcagtgaaa agatcgtagc
    12121 agacatgacc gtagtcgatg attatgcgca tcaccctgca gagatcaaag caacgattga
    12181 tggcgcacgc caaaaatatc cagataaaga aataattgca gttttccaac cccatacgtt
    12241 tacgcgtacg attgcattga tggatgaatt tgcagaagca cttgatttag ctgataaagt
    12301 ttacttatgc gatatttttg gttcggctcg ggaagaacaa ggaaacgtta agattgaaga
    12361 ccttggagcg aaaatcaaaa aaggtggaga agtcatcaaa gaaaataatg tatctccatt
    12421 gttagattat catgatgcag tagtaatctt catgggtgct ggagatgttc aaaaatttga
    12481 acaagcgtat gaaaaacttt taagcagcac gacgaaaaat gtactgtaat acaattactg
    12541 cagtgtcctt ataaaaatag aaagaggggg agtggcaaaa atcttgccaa gccccctttt
    12601 ttgcaactta tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaatcaaa
    12661 tcaaaaacat gagaagctgt tttttcttgg ctgttcttat tatttgatgc aggacagctt
    12721 tttcacgacc tctgtaaact ataggaatct gttaaatcaa gggagcagaa cttgcctttt
    12781 acgctacttt tggtacaata atgtatgatt aaaaataaac tggagggttt ctatcttgaa
    12841 cataggtaag ccgagaaaac taggaaaaac aataaatgaa atcgaggaag gcgactcgtt
    12901 atccttaaca gaatctattg aagataacca gcttttgctg tatttgggat tgacgaatga
    12961 tgcaaatcct ttgtatatcc aacatgactt tgcacaaaaa acagaatatc aacagccaat
    13021 cgttccatca gtgatgctga tggggatcat cacaagtgca atttcaaagc atcttcctgg
    13081 accaggatca catgttgtca atttttctgt caattttatt gaaccggttt tccattatga
    13141 aacactgact tttcagtttg aggtcataaa agtagataag atgaaggatg tggtgacgat
    13201 ttctgtagaa gcggagaata cgaacgaaga tcgtgtgttg gatgctgtcg ttatggtcca
    13261 accgccacaa gttcctgaat tggaaatagc aatcgaaagt gaaggtgaat tgaatgaata
    13321 atccaagtac atcaatgtca ggtatcaatc gtttttatgc aaaagtttat ggatttttag
    13381 gtatgggact tgcccttagt gcagtcactt cttacttggc tctttgggta tttcctttgc
    13441 aattagcaac ttttatcaat aattttccac taggctttgt gggtctgtgg atcgttgaac
    13501 tgattttagt aatggttcta ggcgtaaaag cacaaaagaa tccgagctta gcaatcggtg
    13561 gatttattgc ctactctctt ttgaatggca taatattagc ggttactctt gcatactacg
    13621 atattggaac agtaacgcaa gctttcttga cagctacttt gatgttcatg gggatgtctg
    13681 ttgtcggagt aacaacaaaa agagatctta gcggggttgg aagagcagga caggcagctt
    13741 taatcggagt gatcattgca ctagtcttga acatcttcat tttgagaagt agcgtagttg
    13801 agctgttcat ctccctactg ttagtcgtca tctttgcagg gattacagct tatgataacc
    13861 aaagcatccg taatatctat gtccagacaa acggaacagc acatagtgga attgctgtat
    13921 tcatggcatt gaagctgtat ttggacttta tcaacttgtt cttggcgttt ctgagaatct
    13981 ttggacggaa caactaaaaa agaaaaaaat tcatcagttg gggctatacc gaattatttg
    14041 gtatggtccc gctttttctt ttcgcattcc gcaggattct ttgttagaat gaataagaac
    14101 tttacagtaa ggagttaaga agatgacaaa aaataaatta ttactcgttg atgggaacag
    14161 tgtggctttt cgcgcattct ttgcgcttca caattctctt gaacgtttca aaaatagaaa
    14221 cggtcttcat acaaatgcaa tctatgcatt caatacgatg tttgaaaatg ttatgcaaaa
    14281 agagcaaccg actcatgttc tggttgcgtt cgatgcagga aaaacgactt ttcggacaga
    14341 aatgtatgcg gagtacaaag gtggaagatc caaaacacct ggcgaattca aagaacaaat
    14401 gccatatata cgtgatttgt tgacaggact aggggtacag tattacgaat tgcccaatta
    14461 tgaagcagat gatattatcg gtactttggc agaaaaagta gacaaagatc aatttgatgt
    14521 cgtcgtgctt tcaggtgatc gggatttgac tcaattagct agtaaagaag taaaagtgga
    14581 tatcactgta aaaggtgtca gcgatatcga aagctataca ccagaacatg tagcggaaaa
    14641 atatgatggg ttgacgccaa aacagattat tgacatgaag ggactagccg gagatgcctc
    14701 tgacaatatc cccggtgtga ctaaaatcgg tgaaaaaaca gctatcaagc tattgaaaca
    14761 atatggctca gtcgaaggaa tttatgaaca tatcgatgaa atgaagcaaa gcaaaatgaa
    14821 agaaaatctg ataaatgaca aagagcaggc tttcttatcg aaaaaattgg cgaccatcaa
    14881 tacttcagcg cctgtggatg tctctatcga ctcattgaag tatgaaggga aaaatctcga
    14941 taaactcgtt ccgttttata aagaaatgga tttcaatcag ttcctttcta aattgaatat
    15001 cgtagaagaa gaagtaaaaa tggatgatat tttgtttgaa gtcgttcatg aattcaaaga
    15061 ggaaatgttt actacggata tggcgttgta cgtggaaatg atgggcgaca attatcatac
    15121 agaggaaatc gtcggagtag cgtgggggac agaaaagaaa atttatgtca cgaacgatct
    15181 atctgtattc gaaaaccgag cgttccattc ttggatcact gatccgactc ggctgaaaaa
    15241 agtatatgat gcgaaacgaa cttatgttgc tttaaaccgc tatactggaa aaacgaaagg
    15301 aatcgatttt gatgtcttgc taggtgcata ccttctcgat acgaatgaaa aaagcacaga
    15361 tatcgaagga atagctgctc attacggata caacgatatc caatcagatg aagctgtata
    15421 cggcaaaggg gcgaaaaaag gtttaccaga agaagaggaa ctcttttttg ctcatttagc
    15481 gcgaaaagta gctgcaatca atgcgttgac accaaaattg agccaagaac tggcagaaaa
    15541 aaaccaagaa gatcttttcc ggaaaatgga actgccattg tctatcattt tggcagagat
    15601 ggaaataagc gggatcaaag tagatgcgac tcgcttacaa gaaatgaagg gcgagttctc
    15661 tgctcgctta agagagatcg aacaaaaaat ctacgaagaa gccggcgaag aattcaattt
    15721 gaattcacca aagcaattag gagtgatctt atttgaaaaa atgggattac ccgttatcaa
    15781 aaaaacaaaa actggatatt ccacagccgt agatgtactg gaacagcttc gtgaacaagc
    15841 accaatcgta gaggatatcc tcacctatcg tcaaattgca aaaattcaat ctacctatgt
    15901 cgagggactg ctgaaagtga tccaatcaga tggaaaagtg catacccgtt atgtccagac
    15961 attgacccaa acaggacgat taagctctgt tgatcctaat ttgcagaaca tcccgattcg
    16021 attagatgaa gggagaaaga tacgtcaggc atttgttcct agagaaaaag actggctgat
    16081 ttattcttct gactattcac aaatcgagtt acgcgtactt gcgcatatct caaatgatga
    16141 acatttgaaa gctgccttcc tagaaggtca agatatccat gccagcacag cgatgcgtgt
    16201 atttggaatc gaaaaggcag aggatgtcac accaaatatg cgtaggcagg caaaagcagt
    16261 gaattttggt atcgtgtatg gcatctctga ttacggactt tcacaaaatc taggaatcag
    16321 ccgaaaagca gcacagcaat acatcgatac gtactttgaa aaatacccag gtgtcaaaga
    16381 atatatggaa agaatcgtac gcgaagcaaa agaccaaggc tacgtcgaaa cgctgtatca
    16441 ccgccgtcga tatctgccag acatcaattc aagaaattac aatatccgtt cttttgcgga
    16501 acgaacagct atcaatacac caatccaagg aagtgctgct gatattttga aaatcgcgat
    16561 gatcgaattg gataaacgat taaaagaaac aggtcttcaa gctacgatgc tgttacaagt
    16621 tcatgatgaa cttgtcttcg aggtaccgga aaaagaactt gaatcattag ataaattggt
    16681 gaaggaagtt atggaacaag ctgtttctct tcatgtacca ctgatcactg acagcagttg
    16741 gggaaaaacg tggtacgaag caaaataaac attgtttaag acagagcaat ataatgaagg
    16801 tgaaaagaaa aaatgccaga attaccagaa gtagaaaccg ttcgtaaagg attggaacgg
    16861 ttagtagtag gaaaaacgat tcaaaaagtt caggtcttgt ggcccaagat catcgagcaa
    16921 ccagagacac ccatctttga ggcttctctt gttggcgaaa ccatccaatc cattggacgt
    16981 agagggaagt ttttaatttt tcatctcgat cattgtgaac tgatctcaca cttgcgaatg
    17041 gaaggaaaat atcaatttac gaaagaaaat acaccaatcg ataaacatac gcatgtactt
    17101 ttcttttttg aggatggaag ccaacttcgc tacaacgatg tacgaaaatt tggtcgtatg
    17161 acaatcgtcg aaaaaggagc aagtgcaact taccgaggaa tcatgaaatt gggacctgag
    17221 cctttgccag atagcttctt attagcagat ttcgcaaatg gactgaaaaa gtcccacaaa
    17281 gcaatcaagc cattgctgct agaccaacgt ctagtgactg gtctaggtaa tatttatgta
    17341 gatgaagcat tatgggaagc gaagatccat ccagaacaac cggcgaacac actgcgcccc
    17401 aaagaggtag aacaattacg tctatcgatc attgatgttt tggatcgtgc aatagaagcc
    17461 ggaggtacca cgattcgcag ttatttgaat gcattaggag aatcaggcgg atttcaagta
    17521 gcactgcatg tctatcagca aactggcaaa ccatgtatac gttgtggaac gccgatcgtg
    17581 aaaacaaaag tggcacaaag ggggacgcac tattgtccga attgtcaaag gctaaaaatc
    17641 gcaaagtagg atttgtttta ggtttgacgg gtggaatagc taccggtaaa agtacggcag
    17701 ccaaagtctt tcagtctcat ggatttcctt tgatcgatgg cgatgtgatt gccagagaaa
    17761 ctgttgagcc tggaacgctt gctctgagga aaattgtatc agtgtttggc caagaggtat
    17821 tgcagcctga tgggcaacta gatcgaggga aacttgggat gattgttttt ccatcaaagg
    17881 aaaaacggca aaaacttgat cagctattag atccgtttat ccgaaaagcg ataaaagatc
    17941 aaatcgcctc tctaagttcc aatcatcctt tagtaattgt agatatccca ttgctctacg
    18001 aaggtcatta tgaccactat atggatgcag tagctgtcgt ttatactacg ccagaaactc
    18061 agcttcagcg tttgatgaaa cgtaatcaac tgacgctaga acaagcgcag cagagaatta
    18121 gtagtcaact tccaatcgaa gaaaaaaagc agcgtgcgga tattttgttc gacaacaatg
    18181 gaacaaaaga aatacttgtg ggacaaatcg aaaattggct ttttgctaat cgctttcttt
    18241 gagtatctgt gtcaattttg ctatcctgag tatatagaaa gtgagggata gcgatgggat
    18301 cattaaaaaa tcaaaaggat aaagtcgtag gtaacgcaaa agaaaaaatc ggcgagatgc
    18361 tcaataaaga aaatctaatc gatgaaggtc gtacgcaatc agaaacagca gaagaaaaag
    18421 aaaagcagta ccaagcaaga atcgatgaaa tagaagacca gcaggcagaa gaagaagcga
    18481 agcggcaaga tttgactcaa gaacgaaaaa atccgacacc tgatcgtagt aaaacaccgc
    18541 tcccagaaag tgaccacatt cccgatgagc aaaaaatcga ggaacagcga atgaaacact
    18601 atacaggaat cgaagagaag ttataaaata gtaaaaaatc gagatgtgac ttgaagcaga
    18661 tgagaaggtg ttcatctgct tcaagttttt tgtgctagat gactaaatca gcttttgtgt
    18721 aagttatttc tcagtaatca aggtgatcag tattggaaaa ttgaaattta gtaaggacag
    18781 gggacacaat tacgcatgaa agtagaaaaa attgttctta tatccgtgtt ttttgggtag
    18841 gacagtcgcg tttgtgattt gtttcgtgtt ataatacaac tagttgtaaa gaaagacaca
    18901 tcctttatat aaaatgcgat gcaagaggtg agacaaaatg agatgtccca gatgtcaaca
    18961 taataattca cgagtaatcg acagccgtca agcagatgat ggacgtgcga tccgtcgccg
    19021 tcgcgaatgt gagaattgtt cgttccgatt cactacattt gaacgaattg aagctgctcc
    19081 gttattagta atcaagaaaa acggggaacg cgaagagttc aatcgcgata aaattttaag
    19141 aggactcatc cgttcagcag aaaaaagacc tgtagctatg gaacaaatgg aacaaatcgt
    19201 caaccatgtc gaaaaccgcg tacgcagtct cggagaaaac gaagtatcga cgaatctaat
    19261 tggtgaatat gtgatggaag atttagttga tttagacgaa atcgcttata ttcgttttgc
    19321 aagtgtttat cgccagttca aagatatgtc tgttttctta aaagagttgc aagatatcgt
    19381 ggataaagca aaagaaaata ataaagagaa taataaagaa aacaattaag aaacaaaaag
    19441 gaggcatttc cttggaaaat gcttggaaag agcttcaacc aaaaaatatt taccaaggaa
    19501 gaaaagcggc tccgctgaga gaagacagca agaacgcctt gctgtatctt tatcagccac
    19561 taatcggcgg agaagccttg agtatctatt tcaccctgtt atcagaaatc tctcaagaat
    19621 ctggcaaagg tcctgaaggg atgcatgcag atctgcttag tagtctaggc tgcggtatcc
    19681 cgcaatttta tgatgcaaga aaaaagctag aaggaatcgg cttactggat gtttatttta
    19741 aagaagatag tgatctggga cctcgtttta tttatgagct gttagaacca ctcattccta
    19801 aagcattttt caaagactct gttctggctt ttcttcttct ggaaaaagtt ggtgaacgac
    19861 gattccagaa gcttacccaa ttctttgaac ctaaaaggca tacagcagag ggatatcaaa
    19921 aaatcacaaa aaaattttta gatgtttatc gcttcaatga agcagtgtac gctgctaatc
    19981 aagaaaacat cgaatcgctg aataaatctt ttgaagaatc tgaatcagta gcattacttg
    20041 aagcagattc gacattcgat tggcagtttt tgaatgattg gctggcaaaa aaacatattg
    20101 caagtcttga tgatccaatc atcaaacaag tcgacatgta tcatcagctt tacggtttcg
    20161 atgaattgac acttggcgaa ctgatcagtc aatcttatga ctttagtgaa caaaaagtat
    20221 ctgaaaaaga actgcaaaaa gccattttgg caatgcagag acgtccggtg aagcaaacaa
    20281 aagaaacagt tgcggtttct gaaaacagta atgaaccctc gtcaaacgaa catttgacaa
    20341 acgcagcgct tcagctgata aaagaagcac agagcattcc accgatgaaa tatctggaag
    20401 caatcaagtc agaaaaaggc ggatatgttt ctaaacaaga aaactggtta ctgcaagatc
    20461 ttgtttctca atcaggtctt tccagtagcg tgatcaatgt tttgctgaat tatgtactag
    20521 tgatcaagaa taatgctagc ttaaatgcca gttatgtaaa tacagtggca aatgaatggg
    20581 cacaaaagaa aatcgccaca gcagaagaag cgatccagca cattcggcaa ataagtaatc
    20641 aagcaaaaca atcaaaacaa aagaagcaaa caaattatca aactggcaag cgaaatgttc
    20701 gacgggaaaa gcttcctgat tgggtcaacc agccaaaaga tgaaaaacag atcagcagcg
    20761 agaaaaaagc agagattgac cgccgtttta aagaatacct ggcaaaaaag gagggtgata
    20821 tctagtggaa gacgtaggca aagaattgaa gaggatcatc agcaaacgca attatcaaga
    20881 aaagtatgct gaaatgatcg ctgaagtctt aaatgatgcg gatgttcaag cttttctaag
    20941 cgcacaccaa gaagaattga cacaagcgga tattgaaaaa agttacgcta aattgtatga
    21001 attcgtgcaa gaaaagcgta aatatcaaaa aaacgatcca acgatgatcg ctcccggcta
    21061 tgagccgaag ctgacgttga attttcatta tgtggatgtc acttatgtac caacggaagc
    21121 attgttagca cggcaaagac aagaagaaat cagaagccga gtaaaagcaa tggatatgcc
    21181 aaaagatatt caatcggctt cttttgaaaa ctttgaacgt acagatggac gaggatatgc
    21241 gagcttagaa gccctagatt ttgtagagaa atatactgct gatccaaaag ggtatcataa
    21301 aggactgtat ctagtaggaa gttttggtat tggaaaaaca tatttattag gagcaacagc
    21361 gagagaatta gcgattgctg gattcaccac gacgctagtc cattttccga catttgccgt
    21421 tgagatgaaa caggcaatcg gaaaagatca agtggcagaa aagttagatg cagtgaaacg
    21481 ttcacctatt ttgatgatcg atgacattgg tgcagatgcg atgagtagtt ggatacgaga
    21541 cgaagtattc ggtgttatcc ttcaatatcg gatgcaggaa cagttaccaa ccttcttctc
    21601 atcgaatttt acaatggatg aattggaaca gcatttgtcc gtcactcaac gtggagatga
    21661 agagccgcta aaagcgaaac ggataatgga acgcatacgt tatttgacaa aagaaatcga
    21721 aatgactggt cgcaaccgac gcaatggttg aacagttgac aagtccaact gacatttgac
    21781 ctagtagtaa aagcaggtca gcgaatcatt atatggacag aaatgaccgg agacaatcac
    21841 cgcaacaagt aatgcagaaa cttaaacgga ggatttttta tgagggctat tttttctagt
    21901 atatctgtag ttggtctatt ttttctttta gcaggctgcg gtagcgcaga tagcaacaca
    21961 ggttctgttg attcgattga tgcgggtcaa acagtaaaaa caagcagtac gaaaacggag
    22021 agacaaagca tatttgtcgg aacgattgaa agtatcactc aagtagaagc agaagaagaa
    22081 acgcttcagt tgaaactcgt cgatgtcatg gccaaagaag atccagcgaa tatgggaacg
    22141 tcattcagca atgacggagt gatcttaaat gctactgctg aaaaaataag cgtggacata
    22201 aatgaattga aaatcggtga caaagtccaa tttacattag ttgagatgcc gatcatgacg
    22261 atgtctatcc caccgcaagt tcctggagat tcgattattg atgtttcatt gaacaaataa
    22321 ccaataacat tttttcaaaa ataagaaaat gttgttttat agaacgaaaa ggtatatttt
    22381 ttaaggagat atgggaattt ttttattcct agtctcctta ttttatttgg aaaatgctat
    22441 aattatctag aaggagtgaa gctatgaacc atttagattt atctcagcgc ttagccgcag
    22501 tgggtgactt tgtccctgaa tccgctcgtt tagcagacat tggatcagat cacgcttact
    22561 tacccgttgc attgatatta agaggaaaaa tcgaatatgc agttgctggt gaagtcgttc
    22621 aaggtcctta tgaatcagca cgtaagcaag tcaaaaaaga tggattagaa gaccgtatca
    22681 ccgtacgcct tgcaaatggt ctagatgcta tccagccaca agatggtatt acagctgtca
    22741 cgattgcggg tatgggaggt tcactgatcc gagatatcct agaagctggc aagttgaatc
    22801 aacgcttaag cggacaagaa cgtttgatcc ttcagccaaa catcggagag cgtacattac
    22861 gatcttggct gcaagaaaat cagtataaaa tccaatcaga aaaaatactt gaagaaaata
    22921 aaaaaatata tgaaatcatt gtagccgaaa aagaacaaaa accagttact tacaatgaaa
    22981 tggagctgat gttcgggcca tttctgcttg aagagaaaag tgagatcttc aaatcaaaat
    23041 ggacaagaga gcttcaacaa agaaaagcta tccgtgaaca gctcaaacaa gcagctaatc
    23101 aagaggcacg tcaaaaacaa atcgagcagg aaatccaaat gattgaggag gttttaaagc
    23161 catgacaatc agcggacgca ctttcatcca aaagtttgaa agctattgtc ctcagtggct
    23221 agctgaagaa ggagacccag tcggactgca tatcggaaca ttggataagc caatccaacg
    23281 agtaatgatg acattagacg ttcgtccaga agtggtcgaa gaagcaatca agaaaaaaat
    23341 cgatttgctg attgctaaac atcctccaat ctttcgtcct gtaaaacggc tggttactga
    23401 tcagccacaa gaaaaaatgt atgcagattt gttgaaacat gatattgccg tctatgcagc
    23461 acacacgaac atggatatta tttgggatgg actgaatgac tggttttgtg aacttctcgg
    23521 tattgaagtg gagagttatt tagtaaagac tcatgagatt cattataaaa aactggccgt
    23581 ttatgtacct gtggatcatg cacagaaaat gcgtgaagta ttagctgcag ctggtgctgg
    23641 tacacaaggt gattataccg gtacaagttt tacctcgatt ggacacggtc gatttactcc
    23701 ggaagctggt gcacaaccag cgattgggaa agtagggaag acagaacaag ttcaagaggc
    23761 aaaagttgag gttattttgc cggaaactat cgaaaaacaa gtgattcaag ccatgcggtc
    23821 agctcatcct tatgaggaac ctgcttatga tcttttcgct atagatgaac cagtcgaaat
    23881 gtttgggtta ggacgagtag gtgaattgcc gcaagaaatt tctatagagg catttgtaga
    23941 acaagtaaaa gaagcgttcc aactagatgg actgagaata gtccagccaa aaaatgcaaa
    24001 atcctctgtg aagcgcattg cgatttgcgg tggaagcggt gaaaaatttt atccgcaagc
    24061 aatcgcccaa agagcagatg tctatatcac gggagatatc tattatcata cggctcatga
    24121 tatgcaaagc gctgggttga ttgcaattga tcccggtcat tatattgaat cgttatgcaa
    24181 gcaacgattt atcgagaaat ttgaatcttg gaaacaagaa gaaaattggg atattaactt
    24241 tttcgtttct gaaacaaata caaacccatt tcaatttaat taaaaggaga gaatactatg
    24301 tacgaaaatc ttttgccacg ttttttacga tatgtcaaaa cagaaacacg ctccgatgca
    24361 acaagcaaaa cgactccatc gactcagaca caagttgctt ttgcgcaaga attgaaaaaa
    24421 gaattagaag agttaggctt agctgatgtt ttctataatg aagataacgg attcgttatg
    24481 ggaacattac caagcaatgt tgatcatgaa gtgcgttcca ttggatttat ttcccacatg
    24541 gatacagcag attttaatgc agtaggagtc aatccacaaa tcattgaaaa ctatgatggc
    24601 gagtctacga tcgtactaga tgcggaagaa cgctatacat tgaatacaaa agattttcca
    24661 aatttgaaaa attatgaagg ccacacattg atcacaacag atggcagtac attactggga
    24721 gcagatgaca aatcagggat tgcggaaatc atgactgcaa tggagatctt gatcaataat
    24781 ccttctatcc ctcacggtac catcaaagtg gcttttggtc ctgatgaaga aatcggtgtc
    24841 ggagcggata aattcgatgt tgagcaattc gatgtagatt ttgcttatac agtcgatggt
    24901 ggtcctttag gtgagttgca gtatgaaacg tttagtgctg ctcaagcaaa tattacgatc
    24961 caagggaaaa atgtacatcc agggacggca aaagatacaa tgatcaatgc tctacagtta
    25021 gcaatcgatt ttcataatca gcttccagcc gatgaagttc cagaaaaaac ggaaggatac
    25081 gaaggtttct tccatttgat ggcattggat gggaatcctg aagaagcttc tatgagttat
    25141 atcattcgcg atcatgatcg tgaaaaattc gaagcacgca aagcacaaat cacacatatc
    25201 caagaagagt tgaatcagcg atttgatcaa gaacgaatca aagtagatat gtatgatcaa
    25261 tattacaaca tgaaagaaat cattgaaaaa gatatgagca tcgtggaatt agcgaaacaa
    25321 gcaatgatcg agcttggtat ctctccagtg atcgaaccag ttcgtggagg aacagatggt
    25381 tcaaaaatct cttacatggg tattccgacg ccaaatctat ttgccggagg agaaaatatg
    25441 cacggacgat tcgaatttgt ttctttacaa gtaatggaaa aagcaacaga cgtgattgta
    25501 aaaattgcag aattgaacac aaagagttaa aatcgaaaaa gaacagctta tgacaaggcg
    25561 ttcgtcccaa tgatggaatg tcatctgtct aaggctaaag ggcaggacaa tggaattggt
    25621 tgtcccgccc tcttttttga agagtaaata ggaggtaaag aatggaaaag ctgctagttt
    25681 tttataacaa aagttcaggg aaagatgagg gtgagaaatt agcacgctgg tttcaagatt
    25741 atgtaaaaga aaagcagcca gaattatatg tagaactagc tgaaactggc ccatcgattg
    25801 atgatgcgga attagtcaag aaagcagaag ccatagaagc tgatacttta gtaattatag
    25861 gtggagacgg cacgattcat catatcgtcc aagcgttcca aaaaacatta tctcaatacc
    25921 atgtcggatt acttccaggt ggaactgtca acaaccttgc acgtgttctt gagattcccc
    25981 tcaaaaaaga agaagctgct gacgtgatcc tggaaggcaa tgtacggaaa atagactatg
    26041 gtcaagtcaa tgatgaagtc attatctcta cactgacaat cggaatctta gcagatacag
    26101 cagtttggat cagtcaagaa gaaaaacagc gttacggctc gtggatcttt atcaagaagt
    26161 tttttaagtt attgctgaag aagaaaaaat atcatttgga tttagaaaca gacgaggagc
    26221 attggaaagg aaaatcccaa ctgttgacag tcacgatgtc taactccgtg ggcggtttta
    26281 caaattttga cgactcagcg accctagatg atggaaaatt tcatgtaacg atcgtacctt
    26341 ccttagatat tttccgattt attttttatt tgcctagaat catgagaggc aaattctata
    26401 cgatccccgg cattacttat ttcacagcca aacgcatgaa aatccaagcg caggaaaaat
    26461 ctgtgggcac tcgaacggat ggagacacga cagatgatct tccagttgaa ttatctgttg
    26521 tatcaaaagg attaacagtt tattcaccaa agaaagaaga aaaagcttag aaatagtagt
    26581 taacagaaag ttggaagaag agtaatggat gtattgttac acaatccttt gtggttttac
    26641 cttttcattg tgaattttta cttattttgt ctgatggggt atgataaaca tcaggctaga
    26701 actagcggct ggcgtgttcc tgagaataat ttgatatttg tcagcctgtt aggcggtggt
    26761 ttgggaggat tgatctctcg aaaagttttc catcataaaa ctcggaaaaa gaagtttact
    26821 ataggattcg ctgcaggtgt acttgttgat ttgattctca tttatttttt ccattaagga
    26881 gcaggagatg tcgtctaaag gaacgaaaac taaaatcatt ctgaatctgc ttttgctgat
    26941 tgtgattttt gccattattt attatttaat caatcaatca tttgcagata tctttgccga
    27001 attgctgtct accagtgttg ttgttttggc agcggctgtt atattaggga cagtttacca
    27061 aatcgctgaa gggcgctcaa tcaaagaaat tgctgaacct ttcgcgccga attttacaac
    27121 gaaagatggg ttcttgacct ctagctatat tgcgttttac cgaatcgttt cgtttggtac
    27181 aggaacgctg atctcggaga tttattttta taacaaaaaa ggaatgaagc tatcccaggg
    27241 agcaggggtg acagcactcc atatgattat gtacaaactg gcagtgatca cttatgcatt
    27301 agctggcttg gtcatccaat tctctctttt ttattctaat ggtccatcaa tgatctggtt
    27361 tatcatcgct ggtatccttt tgacgatcgt gattgttgcc tttttattgg cgatgtccgt
    27421 cagcttgaat gttcaaatat tttttgtcag cttatcgaat aagttattta aaagtgagcg
    27481 tttgcggaaa tgggtggata catgcaatca gcagatctac tctttaaggg aaacggtcca
    27541 aaccattatc caagacagga ctgcgctatt gcggatttat ctatggaata tgttaaaact
    27601 tgtattttgg tatgtacttc cttatttgat tctgattgaa aaccatccta aaatagattt
    27661 tctactgacc atttcgttta tcagctttgc agtagtgctt tcaggggtta ttcctacgcc
    27721 tgctggtatt ggttcctttg aatttgtcta tttgttatta ttcagaccag ttgttggaac
    27781 agttgacgca gtttcttcgc ttttactgta tcgtttcagt tcctttatct tgccatttct
    27841 ctatggattt gtgtatgtga ttgctgaacg gcgaagagtg atcaaagaag aaataataga
    27901 agtaaaaaaa gagaataaag aaaatcaagg gtaggtcttt tataagatct acccttttta
    27961 tcaaatcaaa gaaaaacgt
//
LOCUS       DARBDV010000027        27951 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000027 DARBDV010000000
VERSION     DARBDV010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.027"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(114..473)
                     /gene="rplT"
                     /locus_tag="SCM56_001135"
     CDS             complement(114..473)
                     /gene="rplT"
                     /locus_tag="SCM56_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEG1109951.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(526..726)
                     /gene="rpmI"
                     /locus_tag="SCM56_001136"
     CDS             complement(526..726)
                     /gene="rpmI"
                     /locus_tag="SCM56_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEG1109952.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(798..1322)
                     /gene="infC"
                     /locus_tag="SCM56_001137"
     CDS             complement(798..1322)
                     /gene="infC"
                     /locus_tag="SCM56_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEG1109953.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1366..1491)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            1642..2274
                     /locus_tag="SCM56_001138"
     CDS             1642..2274
                     /locus_tag="SCM56_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEG1109954.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2276..3181)
                     /locus_tag="SCM56_001139"
     CDS             complement(2276..3181)
                     /locus_tag="SCM56_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEG1109955.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(3266..3925)
                     /locus_tag="SCM56_001140"
     CDS             complement(3266..3925)
                     /locus_tag="SCM56_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEG1109956.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            4241..5185
                     /gene="mvk"
                     /locus_tag="SCM56_001141"
     CDS             4241..5185
                     /gene="mvk"
                     /locus_tag="SCM56_001141"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEG1109957.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5178..6155
                     /gene="mvaD"
                     /locus_tag="SCM56_001142"
     CDS             5178..6155
                     /gene="mvaD"
                     /locus_tag="SCM56_001142"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEG1109958.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6168..7253
                     /locus_tag="SCM56_001143"
     CDS             6168..7253
                     /locus_tag="SCM56_001143"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEG1109959.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7250..8314
                     /locus_tag="SCM56_001144"
     CDS             7250..8314
                     /locus_tag="SCM56_001144"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEG1109960.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8563..9366)
                     /locus_tag="SCM56_001145"
     CDS             complement(8563..9366)
                     /locus_tag="SCM56_001145"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEG1109961.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="SCM56_001146"
     CDS             complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="SCM56_001146"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HEG1109962.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(10244..11251)
                     /locus_tag="SCM56_001147"
     CDS             complement(10244..11251)
                     /locus_tag="SCM56_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEG1109963.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(11258..11902)
                     /locus_tag="SCM56_001148"
     CDS             complement(11258..11902)
                     /locus_tag="SCM56_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEG1109964.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(12002..12760)
                     /locus_tag="SCM56_001149"
     CDS             complement(12002..12760)
                     /locus_tag="SCM56_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEG1109965.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="SCM56_001150"
     CDS             complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="SCM56_001150"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HEG1109966.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="SCM56_001151"
     CDS             complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="SCM56_001151"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HEG1109967.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="SCM56_001152"
     CDS             complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="SCM56_001152"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HEG1109968.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="SCM56_001153"
     CDS             complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="SCM56_001153"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HEG1109969.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="SCM56_001154"
     CDS             complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="SCM56_001154"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEG1109970.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            18158..19027
                     /locus_tag="SCM56_001155"
     CDS             18158..19027
                     /locus_tag="SCM56_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HEG1109971.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(19153..20052)
                     /locus_tag="SCM56_001156"
     CDS             complement(19153..20052)
                     /locus_tag="SCM56_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1109972.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(20217..21041)
                     /locus_tag="SCM56_001157"
     CDS             complement(20217..21041)
                     /locus_tag="SCM56_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEG1109973.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(21052..22347)
                     /locus_tag="SCM56_001158"
     CDS             complement(21052..22347)
                     /locus_tag="SCM56_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEG1109974.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(22363..22842)
                     /locus_tag="SCM56_001159"
     CDS             complement(22363..22842)
                     /locus_tag="SCM56_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEG1109975.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(22848..23876)
                     /locus_tag="SCM56_001160"
     CDS             complement(22848..23876)
                     /locus_tag="SCM56_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEG1109976.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(23893..24999)
                     /locus_tag="SCM56_001161"
     CDS             complement(23893..24999)
                     /locus_tag="SCM56_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEG1109977.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="SCM56_001162"
                     /gene_synonym="adhC"
     CDS             complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="SCM56_001162"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEG1109978.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
BASE COUNT         7883 a         6132 c         5041 g         8895 t
ORIGIN      
        1 tattttagac ttatacgtta ttgtcctgtt tcatttgatg gaggattgta aataaaaaaa
       61 gaggaaagaa cataactttt cgttatcttc tttcctcttt ataattgttt agcttattta
      121 gccaaagctt cttttgcttg gtcagccaat gcagtaaatg ctgctgcatc gttaacagcc
      181 aagtcagcta acattttacg gttgatgtcg atttcagcaa ctttcaaacc gtgcattaat
      241 tttgagtagc tcaagccatt catacgagct gctgcgttga tacgagcaat ccacaattta
      301 cggaagtcgc gtttcttttg acgacgatcg cgatatgcgt agttgtatga attcattact
      361 tgttcttttg ctgttttaaa taaagtgtgt tttgaaccgt agtaaccttt tgctaatttt
      421 agcactcttt tacgacgttt acgagttact gttccacctt taacacgtgc catgtataat
      481 tcctcctaat aaaaataaat cttcagattc tgttatttgt acttcttatt tcatttttgc
      541 tagttgttgg cgaatacgtt tgaagtcgcc actagaaacc atgcttgctt tacgcaattg
      601 acggcgttgt ttttttgttt tgccgtggaa acggtgactt gtaaacgcgc ggaagcgttt
      661 caatccgcct ttaccagtac gtttgaaacg ttttgctgat ccgcggtgtg ttttttgttt
      721 tggcatgact atatttcctc ctcaaaatct ttcgttatcc gactatcggc tgtccaacag
      781 ccagttagtc attcaaatta cttactgtcg tttttcggtg ccagcgtcaa gaacatgctg
      841 cgtccgtcca ttttcgcttt ttgttccact gttgcaatat ccgcagtttc ttcagctaag
      901 cgattcaaga ctttttgacc aatttcttta tgggtaatgg cacggccttt gaaacggata
      961 gaagctttca ctttatcgcc cttttccaag aacttacgtg cattacgaag ttttgtattg
     1021 aagtcattca agtcgatcgt aggacttaag cgaacttctt ttacattgat cactttttgt
     1081 tttttacgcg cttcgcggtc tttcttttgt tgttcgaaac ggtatttacc gtagtccatg
     1141 attcgcgcaa ctggtggttt cgcaccaggg gcaactaaca caagatccaa gtttgcttgt
     1201 tctgcaatat tcaatgcttc tgcttttgtc ttaactccta attgctcacc atctgaaccg
     1261 atcaatcgta actctcgtgc acgaatgccg tcgttaacca tcatatcctt tgctatggtc
     1321 attcacctcc aaatattatg agagaaagaa aagcttaccg aataaacggc aataaaaaaa
     1381 cgagttctct tacagaaccc gcgcgtactt cacctaatag aaccagtgaa aactatctag
     1441 cccagtgaca ttatcaacta aggcgagaag cgggtgcttc tgcttttatt tctcaaccat
     1501 catagtatag caaaaactaa tctttttgtc aatgtttttc atacagtttg tcttcctttt
     1561 ctgaaaatga ttagattagc agactttcta gcagtgattc ccgcttcttt gctatactaa
     1621 agaaaaacga ggtgaaccca catgaaaaaa atcgaaccta ttccaccttc tcttcctctt
     1681 cttacaaagg gagagtttta tttagaagat gaggagatct tctctggtct tctgatcaac
     1741 gggggtgacc aaagctatct cgatgccaaa aatatggtat taagagagag tcatatccaa
     1801 aaacttacga tgcaaaaaac agttttacgt cattttgaat gcagtaatgt catttttgaa
     1861 aaatgtgatt tttccaatct ggagtggatt ggtggcagtt tccaccaagt cgtgtttcat
     1921 caatgtaaat taacaggtac gaattttgcc gaaagttatc ttcgagactg tactttcact
     1981 gattgtattg cttcgatggc ttcctttagt agtacaaact tgaaagctgt ctccttcgat
     2041 cattgtcaat tagaagacag cgaattttat gaggtcactt ggaaaaacct ttttttgtct
     2101 gacaatcagt tgaccggttc gaactggttt cgaacttctc tgaagggatt agactttacg
     2161 actaatttct tcacagcaat cgctttatct caagattctt tggcgggact aatcgtcaac
     2221 caagagcaag cacttgtgat cgcccaagcc cttggtttgc agatcaaaga ctagatcaac
     2281 gaatccgccg ccacagtgca ataagcatcc aaatagataa gaccaaactg atgatcaagg
     2341 cgattcccca tccgaaaacg gaatgggtaa agggtaactg gagattcatt ccgaagaatc
     2401 ctgtcacaat agttggaacg gtcaagatca gcgaccagac cgtcaaaaat ttcatcgtgt
     2461 cattcaagtt gttattcagc aaattattat aagtgccaga gagctggtct aaaatctgcg
     2521 aagctaagtt cgtcatttca actgcttgtc gtgcttcgat cagcgcatcg tccaactctt
     2581 ctttttcctt ttctgacaac ttgcgataaa tagccaatgc cttgatttgt tctaaaagta
     2641 ctacattttg ttttgttcct gtaactaagt aaaccaaacc gatctctaaa tctgaaagtt
     2701 gcagcagatt cttgttcgtt gttttttctc ttaatttcag attaagccgc tgacgttcac
     2761 tattcacttc ctcgattaaa ggaaaaaagg aatcagaaat caaaaacaat gtacgaaaca
     2821 ataaactgta tacactcatg tctggcattc gttccaaaag cgatcgaatt agtcgaatca
     2881 cgtactcggt attcggcgtg gtaaatgtaa acaaaccgtc ttcttttaaa ataaaagcga
     2941 taggactagt ttcataatga ttatcgactt tttgctggtg cggaacattt gatacaagca
     3001 gtaacgcttc atcaaaagaa tcgtactcta cgcgcgcccg ttcattttta tctagagaat
     3061 aagtcagcat ctcgtcacta atgccatatt cttcttttaa gtttttaaca gctagcacat
     3121 cgttttgctg tacattgatc caaaaattac ggtcttgatt aaaatagtgt ttttcccgca
     3181 tactctatcc tcctgacatt tgtttccagt ataaagaaaa aagtcgctca aataaaggtt
     3241 taccctttat ttgagcaact gtaaatcaaa tggctacttt ggtttggcga atctctgcta
     3301 atttttcttc gatcattttc aagacttctt cttttgcttg aggatcttcc acaaaattat
     3361 agcggtctcc atcgatttgg atttttggac tttctttgta ttcttcatac cattgatcgt
     3421 aacgtctgtt caattctttg taataatcgt atagacttgg atcaaaggac aattgttcat
     3481 acgaacgacc acgtttttcg atacgctcca acatcgtatc aaaagataca cgaatatgaa
     3541 ctaataaatc cggatgtttt ttatgggcag catagggaag ttcttccatc atgttttcca
     3601 gcagttcatc atacacacga acttctgttt ctgttgctct tcctagatct gcatttagat
     3661 ggaaaagcag tgaatcttca taaatcgaac ggtccaatac attattgtca tctttcattg
     3721 cttgtttgat gcttgcaaaa cgtttattca aaaaataaat ttgcagcaaa aaagcatact
     3781 gttcagggtt tgcataaaat aagggcaaga cttcattatc atcaatcgat tcataaaatg
     3841 cctgactatc aaagtgttca gccatcattt ctgttaaact tgattttccg gcaccaattg
     3901 tacctgctaa aacgatcacg ctcatttacc tccagctcac ttcatcacta aatattgtgt
     3961 ttaactccaa cctttttaaa tataccccca atatctagta gtgtcaattg tatttttttc
     4021 tagtcaaaga agaaaaaaag tcaaaaaatt cttaagaaaa aagtgttttt tctttagaaa
     4081 acgtattctt agttttactt ctgagatggt tttagtaaaa taaaggagga atgttttttg
     4141 agacccttag tggacaaaaa aattcgtgaa gcagcgcaac tcgttttggg cgctttattt
     4201 tttgcttaaa aatcactttt acgtgagaag gagttagatt atggcaaact atggccaagg
     4261 agaatcaagc ggaaagatca tattgatggg cgagcacgcg gttgtttatg gagaaccagc
     4321 gattgccttt cctttctatg caacaaaagt caccgcattc cttgaagagc tggatgcaat
     4381 ggacgatcaa ctggtttctt cctactattc aggaaattta gccgaagctc ctcatgcatt
     4441 aaaaaatatc aaaaaattat tcattcactt aaaaaaacag catgacatcc aaaaaaactt
     4501 gcaactgacc attgaaagca cgattcctgc tgaacgtgga atgggatcaa gcgctgcagt
     4561 cgccacagca gtcactcgtg ctttttatga ttacttagca tttcctttgt ctcgtgaaat
     4621 actattagaa aatgtccagc tttcggaaaa aatcgcccac ggtaatccta gtggaatcga
     4681 tgcagccgct actagcagct tgcagccgat ttattttaca aaagggcatc ctttcgacta
     4741 cttttctttg aacatcgatg cttttttgat tgtcgctgat acaggaatca aaggacaaac
     4801 aagagaagcc gtcaaagatg tcgctcacct ctttgaaacc cagcctcatg aaactggaca
     4861 aatgatccaa aaattaggat acttgacgaa gcaagcaaaa caagcaatca ttgaaaattc
     4921 accagaaacg ttggcacaga caatggatga atcacaatca cttctggaaa agctgacaat
     4981 cagcaatgat tttcttaatc tattgatcca aacagcaaaa gataccgggg cattgggtgc
     5041 caaattaact ggcggtggac gcggtggctg tatgattgca ttagcacaaa caaaaacaaa
     5101 agcccaagaa atcagtcaag cacttgaaga tgcgggtgct gctgaaactt ggatacaagg
     5161 attaggagta catacctatg tttaaaggca aagcacgcgc atataccaac attgctctaa
     5221 tcaaatattg gggtaagaaa aatgaagaac tcatccttcc aatgaacaac agcctttcgt
     5281 taacattgga tgcattttat acagagacag aagtcatctt ttctgatagc tatatggtag
     5341 atgaatttta tctagatggc accttgcaag acgaaaaagc aacaaaaaaa gtcagtcagt
     5401 ttcttgacct ttttcgtaaa gaagccggcc tctcactaaa agcttcagta atcagtcaga
     5461 atttcgttcc taccgcagct ggattagcct cctctgccag cgggctagct gctttagcag
     5521 gcgcttgcaa tactgctctt aagcttggat tagatgatct ctctctttca agatttgctc
     5581 gacgcgggtc tggttcagct tgccgaagta ttttcggtgg tttcgtcgaa tgggaaaaag
     5641 gccatgacga cttaagttct tacgctaagc cagtcccttc cgattctttc gaagacgatt
     5701 tagcaatggt tttcgttttg atcaacgacc agaaaaaaga agtgtccagc agaaatggga
     5761 tgcgtcggac agtcgaaaca tccagttttt atcaaggctg gttagattcc gttgaagggg
     5821 atctatatca attgaaacaa gcaatcaaaa caaaagattt ccaacttctc ggagaaacga
     5881 tggaaagaaa cggactaaaa atgcatggaa cgactttagc tgcccagcct ccattcactt
     5941 actggtctcc tgacagctta aaagcgatgg atgcggtgcg acaattgaga aagcaaggca
     6001 ttccatgcta ctttacgatg gatgctggcc ctaatgtcaa agttttagtg gaaaacagcc
     6061 atttatctga ggtacaagaa acatttacca aattatttag caaagaacaa gtaattactg
     6121 cacatgctgg tccaggaatt gcgattattg aataaaggga gatttttatg attgaagtat
     6181 ctgcaccagg caaactctat attgccggag aatatgcagt tgttgaaaca ggccatccag
     6241 cagttatcgc tgcagtcgat caattcgtga cagtcactgt agaatctgca cgaaaagtcg
     6301 gaagtatcca atctgcacaa tatagtggga tgcctgtacg ttggacaaga cgcaacggag
     6361 aattagtttt agatattcgg gaaaatcctt ttcattatat ccttgctgct attcgcttga
     6421 ctgaaaagta tgcacaagaa aaaaacattc ttttatcctt ttatgatctg aaagtaacaa
     6481 gtgagttaga cagttcaaat ggccggaaat atggattggg ttctagcggt gccgtgaccg
     6541 ttgcaacggt caaagccttg aatgtttttt acgcgttgaa tttatctcag ttggagattt
     6601 tcaagattgc agcactagcc aatttagcag ttcaagataa tggttcctgc ggcgacatcg
     6661 ctgctagctg ttatggtggc tggatcgctt tctcaacctt cgaccatcct tggctccaag
     6721 aacaagaaac tcagcattct atcagtgagt tacttgccct ggattggcca ggtctatcca
     6781 ttgagccatt gattgctcct gaagatttac gtttattgat tggttggacg ggtagccctg
     6841 cctctacttc tgatttggtc gatcaagttc accgttcgag agaagataaa atggtggctt
     6901 atcagctttt cttaaaaaat agtacagaat gtgtcaacga aatgatcaaa gggtttaaag
     6961 aaaataatgt aacgttgatc caacagatga ttcgaaaaaa ccgacaatta ctgcatgatt
     7021 tatctgcaat cactggggtc gtcatcgaaa cgcctgcttt gaacaaattg tgtaatttag
     7081 ctgaacagta tgaaggagcc gcaaaatctt ctggtgcagg tgggggcgat tgcggaatcg
     7141 taattgttga ccagaaatct ggcattcttc ctttaatgag tgcatgggaa aaagcagaaa
     7201 tcactccact gccgttacat gtctatagcg atcaaagaaa ggaaaaccga tgaatcgaaa
     7261 agacgaacat gtttcactag ctaaagcctt tcatgataaa caaaaaaacg aatttgactt
     7321 cgtacggatc atccataacc ctttgccgca aatagcagtt tctgatgtcg atctaagtac
     7381 acaggcagtt gggtttacac taagcagccc attttatatc aatgcgatga ctggcggaag
     7441 cgaaaaaaca aaaaaaatca accaagatct tgcaatcgtc gcaagagaag cagatttgat
     7501 gattgccact ggttcagtca gcgctgcctt gaaagtccct tctttagccg atacttatac
     7561 gattatgcga caagaatatc ctcacgggaa aatcattgcc aacatcggtg cagggacttc
     7621 tgtcgaaaga gccaaagaag ctatccgatt gtttcacgca gatgccttac agattcattt
     7681 gaatgcacct caagagttgg ttatgcctga gggagaccgt gattttacca attggaaagt
     7741 acttattcaa gaaactcaaa cagccattga cgtccccctc atcgtaaaag aagtcggttt
     7801 tggcatgaca agagaaacgc tcaatgatct agctgcttta ggagttcata cagttgatat
     7861 cagcggtcga agcggaacaa gcttcaccca gatcgaaaac gcccgtcgct ccaagcggga
     7921 actgagttat ttagctgact gggggcaatc gacagtctct tcattgcttg aagcaaatga
     7981 agcagacact tctatggaaa tcctagcctc cggaggtatc cgcaatgcct atgacatatt
     8041 taaagccctt tgtctcggcg caaatgccgt gggtacttca ggaaccgtac tgacgcatct
     8101 gatgaatcac ggcgtagaag aaactattat actgatgaag caatggcaag aagagcttcg
     8161 tctgctttat actatggtcg gcgcaacgaa taccgcggcc ctacaccaac aatctctgat
     8221 cttttccgga ccagtgaagg attggtgcga agcaagaggg atcgacttag tgaagtatgg
     8281 aagacgcaca gaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
     8341 cacgtacaaa cggcggaagt aaaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
     8401 tttgaaaagc aattttcgtg aattccttct tatttcttgg agttaaatgc ttctgtccca
     8461 gcctcttttt tcgaataaat gatgtttaat cagctgtcct tcggtattcg ttcaatccgc
     8521 tgatttcaca acctctccac taaacagtgt actaaagctc ttttaaggtt gttttccgat
     8581 ataggcaagt atgcctccgt ccacatacaa gacatggccg ttaacgaaat cagatgcgct
     8641 agaagccaag aagacagatg gtccagccaa atcgattggc tcgccccagc gtgctgctgg
     8701 cgtacgtcca acgataaatt gatcgaatgg atgacgttca ccgttttctt gtgtttcgcg
     8761 taatggagca gtttgcggtg tagcaatata acctggtcca attccattac attggatatt
     8821 gtattgaccg tattcagaag cgatgttttt cgtcagcatt tttaatccgc cttttgccgc
     8881 agcataagca ctgaccgttt cgcgtcctaa ttcactcatc atggagcaga tattgatgat
     8941 tttaccgccg cctttttcga tcatatccgg aataactgct tttgaaacga tgaatggggc
     9001 atttaaatcc acgtcgatta cttggcggaa gtctgccgca tccatttcga tcatcggtgt
     9061 ccgtttgatg atccctgcat tgttgactag aatatcaact gaacctacat cttctttgat
     9121 ctgttgaacc atcgcttgaa ccgcttcttc atcggtcaca tcgcagacat aaccttttgc
     9181 atcgatgcct gcttctttat aatgcgctat accttcatca actgattctt gattcaagtt
     9241 attaaatacg atttttgctc ctgctgtagc taaagagcgt gcaatctcaa atccaatacc
     9301 atagacagca ccggtgacta atgctacttt tccatctaaa cgaaacatat ccatactaaa
     9361 ttccatcatt ttctctccct tatgcttatt ttaattgatc catttgcacc atatccatgt
     9421 ctgtataggt gatattttct ccgcacattg cccagatgaa tgagtaattg cttgtaccta
     9481 cacctgaatg gatcgaccag cttggcgaaa taatggcttg ttcattgtct acaactaaat
     9541 gttttgtttc atctggtttg cccatcatgt ggaacacacg tgtatcttca ttggcataat
     9601 caaagtagac ataagcttcc atacgacgtt catgtgtatg acaaggcatc gtattccaag
     9661 cgcttcctgg ttctaaaatc gtataaccca tttgtaattg acagctttcg catacatttg
     9721 gatggatgta ttggtagatt ttacgttcat tcagtgtcag gccttcacca gtctccattg
     9781 gttttacttg gtcgatactg atttttacat ttggatattt atgatgagct ggaacagaac
     9841 taatatagaa tttagctggg ttttcagcat cttgagaaga aaatacaaca tgtcgtgttt
     9901 cttttccgat atagtatccg tcttgttttt tcatttcttc tttttgccca tcgatctcga
     9961 tgaatcctgg tccaccgata ttgatcacac ctagttcacg acgctctaag aagtaatcaa
    10021 cacctagttc tttgtctaat gtgatttcta attctttttc agtaggtgtc actccaccaa
    10081 agatcattcg atcgttgtgc gtataagtca aactgatttt ccctggttca aatacctttt
    10141 ctactaaaaa ttcttttctc aattgttccg ttgaataatg acggatatct tctggactgt
    10201 gtgtataacg tgtttccatt tcttgcattt tcttctactc cttctatcga atgatttttc
    10261 cagagccgca tgctaaaaat tgtgcgactt cttcttgact aaattggttg cagtccccat
    10321 ggatcgtatg cttcaaggca gaagctgccg tagcaaaatc aatgatttct tgcggagaca
    10381 tattggataa aatcccatgg agcacgccac ctgcaaaagc atctccgcca cctactcgat
    10441 ccacgattgg ggtgattcta tggacttggg attccacata ttcatggttc atccacaatg
    10501 tccccttcaa ttcattttca cttgctgaat gaacggtacg cttggtcgaa taaaacacct
    10561 gaatattcgg gaacttttcc tgcatttttt gataataata aattagaggt tgttcttctg
    10621 cttctggcgc tttctcgata cctaacaaat aaagagcatc catttttcca gctgagcaga
    10681 tatctactaa aggtaaaagc tgatggatta cttcgctggc ttcttgctgt gtccataatt
    10741 ttccacgata gttgatatcg aagctgatca ggcagccagc tttttttgca gcttctatca
    10801 cctgtttcgt cattttttgc cattttttag acaaagctgg ggtgattcct gaaacatgaa
    10861 atatatctat tccttggaat aactcctcta gcgaccattc caaatgatcc atttcagcaa
    10921 aacttgatcc ggcacgatca tagatcactg aggcggcgcg ttcgccacta ccagtttcca
    10981 tataataagt acctagtctt gatccaccag ataatacctg tttcgtatct actccataac
    11041 attgcaagtg ctttttgact gcttttccca atgcgttatc aggtactttt gtagcaaaaa
    11101 tcgcttcatg tccgtaatta gctaaagaaa ttgctacgtt tgcttcccct ccgccataat
    11161 gggcagcgaa tcgttcactt gaatcgatac gggttccctg ctctgtggac aatcgcaaca
    11221 tgatttctcc gagtgtcact acttttccca taagcagcta cctcttgatt tctttcattt
    11281 ttgctgcata ctgttgcgcg acttccgtta ctttgtcaaa atctcctgta gctgcgggtg
    11341 ctaaaaggtt cccacctact ccgactgtta cgacaccagc atcgaaccac tctttcatat
    11401 tgtccagact cacaccgcca gttggcatga tattgacttg aggcatcggc gccttgaagg
    11461 cagaaatgat acttggtcca taagcgcttc caggaaatag tttgacgata tctaccccgc
    11521 ttttcagcgc tgttttgatc tcggtaatag tcatacatcc tggtaagtaa ggaatttgat
    11581 acagattgca catctctgcc gtttcttgat caaaggaagg gctaacaata tactctgcgc
    11641 cagctaagat agccaagcga gctgtgacag cgtctaatac tgtgcctgcg ccaatcacta
    11701 tttctggttg gtctttgtag aatgacaaaa gttctttgat tacttgatct gcttgaggga
    11761 ctgtaaaagt caactcaata ccagttagac cacctttgat gattgcgtgg caagcattca
    11821 aagcttcttc tttgcttttt ccgcgtacca ccgcaatcac tccggcgttt ttcaatcgag
    11881 ataaaatatc aatttttttc atcttctcca cctatttcct atttagaaat tttattttat
    11941 aacttggaaa tctataccca tattatacgg cagaaaagaa aaattgcaaa cgcttttttc
    12001 attatttttg aattctttgc tcgatttcgc tttttgtttg cttgagggca ctgattattt
    12061 gttcttttgt ctcatctgtt aaacgatatt tcggcatact tacactaaat gcaccaatga
    12121 tttgttcgtt tttcacgatt gaggctccta tacaaaaaat atcacgttcg atttcttcat
    12181 cgtcaaatgc gatgtgggtt tcttttaccg ttttgatttc ctttttcaac cgtaatgcat
    12241 tggtgatcgt atattctgtg taagggatca acggatgctt ttcaagataa tcagtgtact
    12301 gctcttcact gaattctgct aatacggctt tgcccatcgc agaattgtat aatggtcggg
    12361 tgattccgat ttttgaagac atgcggatcg tttgatgttt tggatctagt ttgttcacgt
    12421 acaagatctc attgttgtcc aataccccta agtggatagt ctcatcgatc ttttgctgca
    12481 gctgttctaa aaacggtaaa gtcaactcta ccaaatcgat ttgttcaatg tttttattcg
    12541 cgtagcgaat aagctttgca cctaaacgat agtttttctc tgaattttta tttacgtagc
    12601 cgatcatcaa caaagtatct agtattttca agactgtaga agaggtcatt cccacaccct
    12661 ttgcgatcgt ctgcaaagag acatcttggt tttccgctaa atagtctagt attttagagg
    12721 ctttgatcaa tacagttcca taaggctttt tattttccat ataatattct cctatttctt
    12781 tataagaaaa attatagcgg aaataccatc aatttaaaag agatatccag atataaacta
    12841 ccatttctct ttacaggcaa tttaatgttg aagatccgct tcgtttttac atcaaacttt
    12901 gatataaagc aatgatttct tctactgtcg tttctcttgg atttcctggt gtgcatacat
    12961 ctcgcaaagc atcttctgca attgctggaa tatctgcttc ttttacacct aattcagaaa
    13021 tggtttctgg gattccaacc gctttgctca accgatcgat ctcaccacaa gccgcttctc
    13081 ttacatcttc taaagacatt tcagctgctc ctttaatccc tagaacaaga gcgatctcac
    13141 ggtacttttc tccagtatat tctttgttat atttcatgac agttggcaaa agtgctgcac
    13201 acgctacgcc atgaggaatg ttgtaccatg cacttaacgg gtgagccatc ccgtgtacca
    13261 gtcctaaacc gacatttgag aagcccattc ccgcaatata ctgtcctaat gccatttttt
    13321 ctcgcccatt ttgatcgcca tctacagaag cttccaaaga atggccgatg atttcgattg
    13381 cttttaaatg cagcatatca gtcatttccc aagcgccttt tgtgataaac ccttcaatag
    13441 catgcgtcat agcatccata cctgttgatg cagccaattt tttcggcatt cccatcatca
    13501 tatcgctgtc gatgaacgcg acgattggga tatcatgagg atcaacacaa acaaattttc
    13561 ggtgatttac ttcatctgtg atgacgtagt tgatcgttac ttctgctgct gttccagaag
    13621 tcgttggtat agctaatata ggtaaacatg gatttttcgt atcagcaaca ccttctaaac
    13681 taatgacgtc tgaaaactct ggattcgtca aaataatccc aatggctttt gccgtatcga
    13741 ttggcgaacc tccaccgatt gcgatcaaac agtctgcctc tgcctcttta acaaaagcca
    13801 ctccatcttt tacattttgg atcgttggat taggtacgat cccgtcgtat aaagcataag
    13861 cgattgctgc tttatctaga agttctgtta cttttgttgc tataccgtgc tcgatcaatc
    13921 ctttgtcagt aatgacagct gctttgccga atcctcgctg ctgaaattca gggacgatcg
    13981 actcaatcgc tccttttcca aaatacgatg tttcatttaa aatcattcga ttactcattt
    14041 tctattcctc ctaatttgta ttatttttca caataaaaac gaaagaaccc caagcaaccg
    14101 gttgcccagt tgcttgttgc tacagttcaa agactggcat caattcccaa ctgactggag
    14161 aggcatctgg atttgtctcc atgatatctt ccatgtactt ccaccatttt tgattgatag
    14221 atgtatttgc cattttttcc cataactttt catcttcgat cactaagtaa ccaaataaca
    14281 aacttgtact cggatcaaga tagatcgaat agtcgatgac cccgtgttca cgaagtgctg
    14341 tacgcatttc tggccaaagt tcatcatgtc tttttttgta ttcttcttgt ttttctgggt
    14401 aaactttcat gcaaaaagct tttttcacca tgtcagacac ctgctttcgt atgaacttcc
    14461 tccaaataac ctgcttttgg agtgacacca aacgcatctg ctaaatgcca caggttttcg
    14521 tccgtgattg tttgcaagat tggtccttgc gctttgacgt acgtatagac ttctgctgcc
    14581 ttttctgctg tctcgatcaa accaaagact tcatccatgt cttttcctga cccgtagatt
    14641 ccatgttgtg gccataaaac caatcgagtt tctttcattt tttcagctgt tgcttcccca
    14701 atctcatttg ttcccggaac catccacgga atgatggaaa ttccttctgg aaaaacaact
    14761 aagcattccg tacacatttg ccatagcgta cgactaaagg aacgttcgtc taattcatgc
    14821 gtaaaactca tcgctaatag atgactagca tggcaatgca taacgatccg attatctggg
    14881 tctacttcta aacgggcgat atgactcatg aaatgacttg gcaactcgct tgtaggaagg
    14941 cattcgtttt ccagccccca caaaagttcc agtgttttgc catcttcagc aactcgaacg
    15001 attccaagat tctcggcagg ttcgctcgct acatttttaa aatatcttcc tgaaccagtt
    15061 acaataaaat agcatcctgc taaacggctg gcatcaaaaa tcatcgagat gcggcggatc
    15121 actttttcag gatccaagaa cggtgtgatc tcttcttctt ttagtaaata agaaatattc
    15181 ccgccatttc tttcatccca tcccaagcga taaagattcg acgtcgtttc gatcatttct
    15241 ttcacataag gcgcttgtaa aatatctatt tttttcaata tagccacact cctattttct
    15301 taattccaac acgtcttttt gatattcctg gacttcttcc agccaatcca aaccaactgg
    15361 aacttgattt ttgtaacaga aataattcca tacatcggca tacgggaaat ctttcaattc
    15421 ttccgtataa gccattcttg aagtaaagtc aaaagccagc tccatttctt tcaatcgctc
    15481 aactggttcc agcatagctt tcagcagtgc tttttgtgta tttctcgtac cgatgaccca
    15541 agcagcgata cggttgatcg ttgcatcaaa gaaatccaga ccgatatttg ttttttccaa
    15601 caattcgtta cggactaatt ctttagcgat ttcctgcaat tcatcatcca tcaaaaccac
    15661 atggtctgaa tcccaacgca ctggacggga aacatgcaac atgattcctt tgctgaataa
    15721 agctaatgaa gataatttat tggaaatcac ttctgttgga tgaaaatgtc cagcatctag
    15781 acaaacaagt ttgtcacgag tcaacccgta acccatataa aattcatggg aaccgactgt
    15841 ataagcttca gcgcctaaac caaatagttt gctttctact gcatcttgta cataccgttc
    15901 atctaactct tctgtaaaaa tttcatcgag tgattccatt aaacgttccc gtggggtaaa
    15961 acgatcaatc ggattatctt tcatcccatc aggtacccaa aagttattga tacttgtttg
    16021 cccagtttct ttcccaaagt actccgcgat tttgcggctt cttttcccgt gttcgatcca
    16081 gtattcacga acttctttat ctggatgagc caatgtaaag ccgtctttca tcattggatg
    16141 cgaaaagaaa gtcggattga agtcaagacc gatttcttct ttttttgccc actctaccca
    16201 tttttcaaaa tgtttcggct caatttcatt tagatcgaca gattcttctg tatccaaata
    16261 gatagcatga aggttcaatt tgtgtttgcc cggtatcaac gaataagcct tctccagatc
    16321 ttgtctaagc tcttcaggtg ttcgtgcggc tcctggaaaa ttccctgtcg ccatgattcc
    16381 accggtcaat tcgccatccg gtgtcaagaa acctttgaca tcatctcctt gccagcaatg
    16441 catagaaatt ttgatgtcct gtatcttttc tagtacagca tctgtatcta caccgatagt
    16501 tgcatatttt tcttttgctt gttcgtaatt tctttcaatc gtgttcatac gattactcct
    16561 ttgattttat tcacgtacca ttccatcgtg ttttgcttat tttcatctgg gtaaaaagtt
    16621 tcacatggaa acgactgttt gattacattt cttgcttgtt ctatattcga aaactgacct
    16681 gtactgagca tttgcatggc aacattcccg attgctgtgg cttcactagg tcctgcttct
    16741 acaatgattc cggcataatc tgctgtcaat tgattcagta gacgattatt cgatccgccc
    16801 cctacgatgt gcagcttttc caatttcgtt ccagttaagt gttctacttt gatcagctct
    16861 tgtgcgtaac aaagagcaag attatcatac acacaacgag cgatagctcc cgatgagaca
    16921 gggactggct gctctgtttc aaaacagtat tgctgaatct cttctatcat atttggggga
    16981 ttcaaaaatc gagggtcatt gatttccatc acttgctgca gcggtttttc ttttgcagca
    17041 agctctgcca attccgcata actgtactcg tactcttgca ttcgggcaac ttcctggatc
    17101 aaccacatcc ccatgatgtt tttcaaaaaa cgcaccgtgt tatgggcacc ccattcattc
    17161 gtataatttt cagaaaaagc attgcgagaa acattggcga tctttgtttc aatccctaaa
    17221 agcgaccatg tcccactgct gatataaccc caacgttctc cttgacctgg cgttcctaat
    17281 acagctgacg ccgtatcatg acttgctact gtgataaagg tcacatctgg caaatcatat
    17341 tttgaaaatt gttctttttg cagatctcct agtatcgttc ccggctctgt caatggtgga
    17401 aataattcct tcgagacacc gattttttca agaagttcta tttcccattc ttttgtccct
    17461 gcattcagca gctgcatagt agatgcatta gttttctccg tcaccatgtt accagtaaag
    17521 acatacccta aataatcagg tatcagtaac aatttatgtg cttttgccaa attttctttt
    17581 tcttcagcaa acaattggaa caaggtattg aaaggctgga tctgaatccc tgtctgctca
    17641 tacaacttat ctagagaata gatttcagaa aactgttcta ctgctccatt ggtacgcttg
    17701 tcccgataag caatcggatc atttatccga tccccttgtt catcaatcag acagtaatcg
    17761 actgcccatg tatcgatccc cgcaaaaagg cgatcaatcc ctgtctgctt taatttttca
    17821 aatccaatca aaatctcttt gataagataa tcgatcttcc agcgatcgtg accgtctttg
    17881 ttccgaaagc cgtttttgaa ccggtgtact tcttccaaga tcaaagaacc ttttgcatcc
    17941 ctcgtacttt tcattagtcg tccgcttgat gcaccgatat cgatggcaac aaatgttgga
    18001 ttcatttctc cacctctttt actttattgt tgattagcac tttattaata aagcgttatc
    18061 atttattgac aatgctatca taatacagaa tcttatattt tgcttcacac tattttggta
    18121 tataattgca ttatcttgac aagttgggag tgtttacatg gattactggg aaaaagccaa
    18181 ccactcatgg tcggaagact caaaaagagt gatcttgacg cctacacaaa gaagcaggaa
    18241 tttatttttt tatatccaag aaatcggtta cttcaaagca ccaatccctt actatactga
    18301 acgagcacat ttaccttctt acttgataaa gtttactttg tctggcaaag gttcgcttac
    18361 atataatgat aatcagcaac agctaaaagc tggagactta ttttttattg actgccaaga
    18421 atatcagcgt tatcaaacga tcagcgaaga accatgggaa atggattgga tccatttgaa
    18481 tggtcagaac gtccaatcat tttatcagga gttcacaaaa gacggccata acaccttcca
    18541 ttcaacagga gatccatcag aaaacaagat ccaccatttg atcacagaat tgatccacat
    18601 gcaacaaggc acgaatgccc gtaccgattt ccagtcctct gtagctatcc acgagttgct
    18661 gaatgaactg attttgcaaa agtatcagct agatttcgaa gaagaggata ttcccgccta
    18721 cgtttacaaa ctgaagcaat acttagacga acatttcaaa gaaacgatta cacttgatca
    18781 attagaacaa caattcttat tgaataaata tcagctgagc aaagattttt ccaaatttat
    18841 cggttctcca ccaatcgatt atttgatcag caaaaaactt tcttacgcta aagaccttct
    18901 tcggtataca gaatttacgg ttcgaatgat ttccgaagaa atagggattg agaattttgc
    18961 ttactttagc cgcctgttca aagcaaaaac aggactatcg ccgagagact ttcgtaaatt
    19021 cagctgattc atataatatg tacgtcattt tttcaaaata ttttgaaata tatagataaa
    19081 aataaaataa ccgcagtttc tgcttctatg taaacaacag cagctactgc ggttattttt
    19141 tagtgatcat tctcattgtt catcttcaaa atgttcataa tgaaccaact gtttctgtgt
    19201 atcacgtttc aaaaattctt taggcgttat gccgaactct ctcttgaatg ctttcagaaa
    19261 atgcggataa gaagaaaaac cacttttact atatacagca ctagagctgc ggtattctcg
    19321 caacaagtgc ttggcataga tcagcttttt cttcattaca tactgatgaa acgtaaatcc
    19381 agtgtacttt ttaaattctc gtgtgatatg gtatttgctg acaaaaaact gcttttccat
    19441 ttcctctaaa gaaagcggat gagataaatt ttttgagata tattgtaaca tttgttccac
    19501 acgctcatta ggaactacag cattctgttc ttgcgcactc tcttcttttt gtacaacttg
    19561 attgagaaaa atcaagtatt ctaccagagc ttgttcatat tttatgtctt caccgtaaat
    19621 ttttttatca ggaggatggt cgataaatga taatttttgc ttcaatactt cctgttctat
    19681 ttgcaataca cgactcctct tctctcccat tggttgaaaa caagccgata gattagacca
    19741 tggcgtggaa cgactttcta gaaaagaagg ggtgacaaag atatagacac gctcgaatac
    19801 atctgaagat tggcggacga tacgatgcaa atcattggaa tggatgagca gcacacttcc
    19861 tgctttgatc gtaaattgtc gtccgttcaa ataaaaagtc gcttctccct ttaatgtagc
    19921 atggatctca tagaaatcat gatgatgata gatcgtttta ttttggctga cgacatcttt
    19981 gacatgatag atttcatact gatcagaaac catcgtattg acttgttcac cgctgatttt
    20041 cttttggagc atgtgccgca cctccttttg ttcacttatg cacaatttta ttataccacg
    20101 ttttttgtaa tcaaaaagag ctgcttttat atactcttat aaaaaagaag agacgtggca
    20161 tcttctagcc ccgccccctc cattctaaat attagttaac caacgtcttc tttacattat
    20221 tcaagaagac ttcttccata acgaatccca tcatcttttg tttcttcaag aaccacatac
    20281 aggtacggac gattcttcat gatgatttct ttgatttttg tgtactcgat cacaccgtct
    20341 cccaaattta ccggcacgat tttttcctgt tcgacacgaa aatctttcaa atgaaccgca
    20401 catatctttt caccaaaacg ttcaaaagct tcttctacca attggacttg ttcttgataa
    20461 tcactacttg tgatcaaatt ggtcggatct aaaatgattc ctagatagtc cgaatctact
    20521 gcttggatca gctgctctac ccttgctaaa gaatacaccg gatgattcaa acctggttct
    20581 atccccacca tcatttggta tttttctcca gcttttacca agcgacggat aacggacaca
    20641 gcttcagcaa atgcttcatc tgtgaaattt tcttccgtat attggatttc cggcaaaaca
    20701 catccagttt ccgaagcaac catcgacgca ccaaaggaag aagcatagcg aagatactgc
    20761 tcgaactttg tcagcaactc ttgtcgtatc gttaaatctg gatgaatcat attgatatag
    20821 caacttaaaa ttcctacggt cacttcttgc ttttctaaaa ctcgtttgac ataatttccc
    20881 atcccagaat tgatctcatt tgcgccagaa ggtatttcag gaaacgaaag acctaaggcg
    20941 agctgcagtg tatgaatccc ttgttctttt gttttttctg caagatcttc tatcgtttgg
    21001 acttcggaaa gatcatgtcc tctaattgta atgttcaaca taactattct cctaactcag
    21061 catattgggt agccacatga cgatttgcgg gaagtaagta atcaaaaaca agacgactaa
    21121 gataacccca taaaaaggaa gaagcggttt cagcattgcc tctgctttta cgcctcctac
    21181 acttgcaccg acatatagtc ctgtacctac tggcggcgtg atggagccta cacacagatt
    21241 catgattgaa aacaacccgt aatgtacagg atcaacccct acacttgtga cgattggtaa
    21301 aaatattggt gtaaaaatca agatagcagg cgccacatcc ataaacatac ccaccagtaa
    21361 taacacaaga ttgactaata acagaataat aaatttatta tccgttacgc ccataatcaa
    21421 cgaactaatt gcctgaggaa taccggtaaa cgccatggcg aaagacatca tggaagaagt
    21481 agccaataag aacatgatcg tccctgacat tgctactgat tgaaacaaca tattaggtat
    21541 atcttttagt tttaccgttt tatagtaaaa catagcaatc aacagggagt aaatgacagc
    21601 tatcgctgaa gcttctactg ctgtaaatat ccccgtcaag ataccaccga taatgatgac
    21661 aatcaataga aggctaggaa tcgcctcttt tattattttt ccagcatgag ccatctgtcc
    21721 tttttcgaca acagggtatt tattcttttt agcaacgatg aatgctacta gcatgattcc
    21781 tagcgcccac aaagcgccta cgagataacc tcccataaac aaagcagaga tcgaagcacc
    21841 accagcaaca agtgaataca tgataaaagc agtacttgga ggaatgacca tacctgtcgg
    21901 agctgacgcg atattgaccg ctgtcgcaaa cttccggtca tatccttctt ccacttgctg
    21961 agggatcaac actccgccaa tcgcggtaga agcggcaatg gccgagctag agatggaacc
    22021 aaataaagca ttccctaaca cattcgtatg agccaaagcc cccggcacgc gcccgacgaa
    22081 caacatcgca aaatccacta gtttcttagc tatccctcca ttattcatga taatgcccga
    22141 gagaacgaaa aacggaatag cgatcagcga aaaactattg atacttccca ccattttttg
    22201 agctgaacta aaagcagaaa catcaaacgg tataactaac aataaagtca tcatcgatga
    22261 gaatgcaata cttatagcaa tcggcatccc taatacaagc aacacaacaa atacaaaaaa
    22321 taacactaat ccggcttcta ttgccatttt attcctccat ttttatattt tttcttgaat
    22381 tgctggcttg ttgcttaatg tatcgattag attgatcagg ctatagataa tgatgagtac
    22441 ccccgataca ggtactgaca gataaacata tcccatagga atacttaaag aaggcgagat
    22501 ctgccccatt gtcaatgtga cagcattcca tccgccatac accatgatca tcgcagcaaa
    22561 taccagaaat aaactttgaa caacaacttc taaaatttta ctttttttgc cagttaattt
    22621 ttcacttaaa accgttacgg ctaaatgtgc tttttgtccc acaacatacg cggaagacaa
    22681 catggcaaac cagacaagac cgaatcgaac aagctcttcc gtgaccgtat ttggatttcc
    22741 taaaaccgtt ctggaaaata cttgccataa aacaacagcg accatcccaa caagcaaaac
    22801 agctcccagg atctctaata ccttgtttaa aatcattttc atcacagcta cgctccttgc
    22861 tcttggatct tttgataaag gtctttgtag gtaggatcgt ttttgaatat atcatgaagt
    22921 ggggcaactg cctctcgaaa cggtgtatta tctacatcat tgaattggac tccatgttct
    22981 tcttgtgcta aagctttttc ttcttcaatt gcttttgcaa aagcttcaat ttcaaaaacc
    23041 gttgattctt tcgctgcttc ttcaatggct ttttcctgct catcagttag ctgttctttg
    23101 atggcatcat tcatgatgat gatatctggc acacgtgtat gctcatcata ggaatagtat
    23161 ttagccacac caccatgtcc agctgtatac aagacaaatt cattgttttc tgatccgttg
    23221 atcaaattgg attgaagaga cgtgtacact tcatcacttc ccataggtac gggcgaaccg
    23281 cctaaaagct ctaccatttt gatagcggtt tcgctttgca tcacacggat cttctttccc
    23341 ttcaaatctt ccggcgtatg gatcggaccg tctaccatat aaaagctgcg ctgaccagaa
    23401 tcataatagg tcaatccggt aaatcctaaa tttttggtcg attggtagat tggctccatt
    23461 atttcttcat tatccatcac cttaaagaaa tgttcttcat cattgaatag ataaggaact
    23521 gaaaaaatcg aataatcttt agagaaactt tccaacgctg aggcacttac ttttgtgaaa
    23581 tctactgctc ctgactgtgt caattcgatc agttcacgtt ctccacccag ttgtccatct
    23641 gggaaatact ggatctgtac ggaaccatct gttttttctt ttactaaacg cccaaattca
    23701 ttcattgcat caattactgg ctggctgttg cttgcataag caaagcgcaa ggtaactatc
    23761 tcatttacat ctgcatctgc gacagttgcc acactagaat tatttccgca gccgcttaga
    23821 cctagcagac ctaggctaag cgaacttgct gcaaacagca gtgatgtttt tttcatatgg
    23881 gtttcctttc ttttaacaat aagagacgag caattctgtc aaacaaagca ccgttaacga
    23941 ctgtccataa ggcattgctg tgattccgat attccgataa aaatctaaac tgtcccccat
    24001 tcctgtcccg attgaaacgt tttgaacttc tcctttttca tcgatttgtt caagcaatcc
    24061 ttggatagca cgataagcga cttctttata ttcttgtggt aaataacgtt tatggaccgc
    24121 tttcaaaata ccgtaagcaa atcctgcagt tgcagaagat tctacgtaag aagaaggatc
    24181 atccaacaac gtatgccata aaccgctttc agactgataa gtttttaatg cacgcacttg
    24241 tgcttcaagt gtttcgatca ataatttgcg caatgcatct tctttcgata attctaagat
    24301 ttcaatgatc tctggaatag caatcgtcaa ccagcagttt ccacgagccc aaaatgcttc
    24361 ggcatagtta tgatttcctt caaacgtcca gccgtgatac cacaatcctg ttcgtttatc
    24421 catcaagtat ttgatatgga tcataaactg gtaacgagct tcttctaaat attctgggcg
    24481 attcaacagt ttcccgattt ttgccaaagg aagcactgtc atcatcaatg tatcgtccca
    24541 caactgattc ttattttccg gtccatatgt ggcatgctgt aacccgtctt catttgtccg
    24601 aggcatgtca ttcatgaccc attctgccca ttgttctaaa taaggcaaat agcgtgaatc
    24661 tttcgtgtct tcatacaagt aagccatagt caatagcggt gccattgtat tgacgttttt
    24721 gggcggagcg ccttcttcca tacgatcctc aaaccattca ttgaccactt gataagcgcg
    24781 aggatttttt gtcatctggt aatttttata aataccatat agaccgacac cttgtggcca
    24841 attccacact gtccagcttt tgtcatctac tttcagtcca tcaaagtcca acaaaaattc
    24901 accattatcg tctttgatcg ttgtcaggtt ttcaacgagc aggtcaattt tttctttcac
    24961 taattcttta ggtacaaaaa tctcttcttt aataatcatc tttacgccac ctttttaaac
    25021 gctttcattt tatatcctta atatagcagt aataccgtcc actataaata tcgctttttg
    25081 aacaaaaact gtctaaaatt gacctaaatg ttaaataaca aaagtaactt tcagttactt
    25141 ggaaattaaa aaaagaactt ggagaaacaa cgttttccaa gttctttttc atctattttc
    25201 ttatcttttt tctacgcttt cttttgctta tccgttgtaa gcatcaatga tgatctgttt
    25261 caattcagag atcaatggtt ctttcggatt agctgtcgta cattggtctt cgtaagctaa
    25321 ttcagccata cggtctacgg ttgtatctag tgtttcttgt gtgactcctt gagcttttag
    25381 actcatgttg atgccgacag attttcctaa ttcataaaca gccgttgcta aagcttcgac
    25441 taattcctct gttgtttcgc ctttcagacc taagaatttt gcgatatctg cgtaatctgt
    25501 atctgcacgg aaatagtcat atttagggaa catcgcatgt ttttgtggat cttttgcatt
    25561 atagcggata atatgcggta aaagaatcgc atttgtccgt ccatgaggga tcccgtattc
    25621 cccgccgatt ttatgtgcga ctgagtgaca aatacctagg aatgcgttag caaacgccat
    25681 accagccatt gtagaagcgt tgtgcatttt ttcacgtgat tccatatcag gtgtttttac
    25741 tgatttttct aaatagtcaa agactaactt gattgcttgt aggctcaatc cacgtgtata
    25801 atctgaagcc atgactgaaa cataggattc gattgcatgt gttaacacat ccatcccggt
    25861 atctgctgtg acagatgctg gaactgacat gacgaattga ggatcgacga ttgcgacatc
    25921 tggcgtcaat gcataatctg ctaacggata tttcacatgt gtttcactgt cagtgatgac
    25981 cgcaaatggc gttacttcag aaccagtccc tgatgttgtc gggatacata cgaattgtgt
    26041 tttttctggt ttgtcgatct tatatgtccg tttacggata tccaagaatt tttgttttgc
    26101 accaaagaaa gatgtgtctg ggtgttcata gaacatccac attcccttag ctgcgtccat
    26161 tgcagaaccg cctccaagag cgattactgt atctggcttg aagtctgcca tcagtttcgt
    26221 tcctgcgtat acggtatttg tcgatgggtt cggttcaaca tctgagaaga cttcgatctt
    26281 cacatcattt ttacgtcgtg ccaatacttc acgaactgta tcgcagtaac cgaattggac
    26341 cataccaggg tcacaaacga tcatgacacg ttcaacgttt tccattttct ctaagtacaa
    26401 tagagagttc ttttcaaaga agatttttgg cggtaatttg aaccattgca tgttatttct
    26461 ccgtttcgct acagttttga cattgattaa gtttacagca gagacgttac gagaaacaga
    26521 gttcttacca taagaaccgc atcccaaagt caatgacgga atcatttcgt tatagatatc
    26581 accgatgccg ccttctgcag atggtgtatt cactaaaata cgacaagctt tcattcgaag
    26641 accgaatttg acatgcaact cttcgttttc tgaatggata actgctgtat gtcctaatcc
    26701 tccaagattc aacatacctt cacacaaatc aaacgcatgg tccgtgttgt tagctttgat
    26761 catggcaaga actggagaaa gtttttctct tgatagcgga taatctgggc ctacgccttc
    26821 caattcagca atcagcattt tcgttccttt tggtacactg attcctgcca attttgcgat
    26881 ttccatcgca gaatgtccaa cgattgatgg attgaccgca tattttcctt cgttcatcac
    26941 tgcatcttct aatttttgca attcatctgg ttttacgata taaacttggt gtgcttggaa
    27001 ttctgctttt actgctgcat agatttcttt atccacgatc acagcttgtt cagaagcaca
    27061 aatcattcca ttatcaaatg ttttcgaaac gatcaaatcg ttcactgcac gtttaatttt
    27121 tgctgttttt tcaatataag ctggtacgtt acctggacct acacctaaag ctggttttcc
    27181 tgtagagtaa gcagacttga ccattccagc tccacctgtc gctaagacga tcgcgatacc
    27241 tggatggttc attagcatag aagtcgcttc gatcgaagga tgttcgatcc attggataca
    27301 attttcagga gctccggcag cgatagcagc atcacgtacc actcgtgctg cttctgcaga
    27361 agatttttgg gcacttggat ggaaagcaaa aacgattgga tttcttgttt tcaacgcgat
    27421 cattgatttg aaaatcgttg tggaagtcgg attcgttgtt ggagttactc cgcaaaccac
    27481 accgactggt tcagcaattt caatcaaccc tttttggaca tcttcattga tcacaccaac
    27541 tgttttatca tgtttgatat tatgccaaat atattcggac gcatacatat ttttgattgc
    27601 tttatcttcg taaacaccgc ggcctgtttc ctctacagcc atcttcgcta atggcatatg
    27661 ttgatccaaa gctgccattg ccatttcatg cacgatatgg tctacttttt cttgatcaaa
    27721 gctttccatt tctttcaatg ctgcatttgc tttttctgcc aaatcatcaa tcattgcttg
    27781 tacgtcaatt gtcttcgttt tttctttttc tattacttta gccatcatta tcctcctaat
    27841 gggtcatggt tatttgttaa ataattcaca tgtatatcat acaatattta tttttctatg
    27901 taaacacttt tttgtgattt ttttcacagt tatttttaaa tataaaattg c
//
LOCUS       DARBDV010000028        27851 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000028 DARBDV010000000
VERSION     DARBDV010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.028"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(242..1186)
                     /locus_tag="SCM56_001163"
     CDS             complement(242..1186)
                     /locus_tag="SCM56_001163"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEG1109979.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1482..2426)
                     /locus_tag="SCM56_001164"
     CDS             complement(1482..2426)
                     /locus_tag="SCM56_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1109980.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2647..3861
                     /locus_tag="SCM56_001165"
     CDS             2647..3861
                     /locus_tag="SCM56_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1109981.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3883..5898
                     /locus_tag="SCM56_001166"
     CDS             3883..5898
                     /locus_tag="SCM56_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HEG1109982.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5981..7354)
                     /locus_tag="SCM56_001167"
     CDS             complement(5981..7354)
                     /locus_tag="SCM56_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEG1109983.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7380..7664)
                     /locus_tag="SCM56_001168"
     CDS             complement(7380..7664)
                     /locus_tag="SCM56_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1109984.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7666..9681)
                     /locus_tag="SCM56_001169"
     CDS             complement(7666..9681)
                     /locus_tag="SCM56_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEG1109985.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9860..10162)
                     /locus_tag="SCM56_001170"
     CDS             complement(9860..10162)
                     /locus_tag="SCM56_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HEG1109986.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEVALAGTNSGETEDKEESLAGTN
                     SGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10251..11282)
                     /locus_tag="SCM56_001171"
     CDS             complement(10251..11282)
                     /locus_tag="SCM56_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG1109987.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11489..12364
                     /locus_tag="SCM56_001172"
     CDS             11489..12364
                     /locus_tag="SCM56_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG1109988.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12516..13358)
                     /locus_tag="SCM56_001173"
     CDS             complement(12516..13358)
                     /locus_tag="SCM56_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEG1109989.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13450..15327)
                     /locus_tag="SCM56_001174"
     CDS             complement(13450..15327)
                     /locus_tag="SCM56_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEG1109990.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15324..16745)
                     /locus_tag="SCM56_001175"
     CDS             complement(15324..16745)
                     /locus_tag="SCM56_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEG1109991.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16748..20137)
                     /locus_tag="SCM56_001176"
     CDS             complement(16748..20137)
                     /locus_tag="SCM56_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEG1109992.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20394..21785
                     /locus_tag="SCM56_001177"
     CDS             20394..21785
                     /locus_tag="SCM56_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1109993.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21858..22505)
                     /locus_tag="SCM56_001178"
     CDS             complement(21858..22505)
                     /locus_tag="SCM56_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEG1109994.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22513..23199)
                     /locus_tag="SCM56_001179"
     CDS             complement(22513..23199)
                     /locus_tag="SCM56_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1109995.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23205..24278)
                     /locus_tag="SCM56_001180"
     CDS             complement(23205..24278)
                     /locus_tag="SCM56_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1109996.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24500..25174
                     /locus_tag="SCM56_001181"
     CDS             24500..25174
                     /locus_tag="SCM56_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1109997.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25179..26519
                     /locus_tag="SCM56_001182"
     CDS             25179..26519
                     /locus_tag="SCM56_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG1109998.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26668..27654)
                     /locus_tag="SCM56_001183"
     CDS             complement(26668..27654)
                     /locus_tag="SCM56_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HEG1109999.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8125 a         5252 c         5052 g         9422 t
ORIGIN      
        1 cattgtattt gccgttgcgg aaggattttt taattaacca cagataaaca ttgataacga
       61 ttcgcttatt gtaatcaaca gattaaattt catatgttca tatgtacgga tacattttcg
      121 gaccgattaa ttttaaccat tctgtttatt tgcttaccta ttagatatca ataaaaaact
      181 gcttaacagc tatttcagct gttaagcagt tttaggattt ttagttcata ttcattgttt
      241 cttataatac agtgttcaat acttctctta aagcacttgt tgatttgctg aacattgctt
      301 tttcttcttc gttgattgat aattcaacaa cttcacgaac accattttcg tcaacgattg
      361 atggcacacc tgtaaagata tctttttcgt cgtattcacc agttaaataa gcagatacag
      421 gcaatacagc ttgttcgtta ttcaagatag cttttacgat acgtgcagta ctcataccaa
      481 taccatagta agttgctttt ttacgatcaa tgatctcata agcagcatta cggactttat
      541 cagcaatcac atctaattca tcttgggcaa tacgatggtc tttttctaca atttcaaaca
      601 ctggtttacc accgacagtt gtatgtgacc atgcagcaac ttctgaatca ccatgttcac
      661 ctaagatata gccgtggacg ctgcgtgggt caacttttaa tttcaatgcg atttctttac
      721 ggaaacgagt tgtatccaat gttgttccag taccgataac acgtgatgtt ggcagaccag
      781 attcgttcca agcgatatat gtcaaaatat ctactgggtt tgaagcaaca acgataatac
      841 cgtcgaatcc tgatcccatg atttctttta cgatttggcg catgattgac gcatttgttt
      901 taactaaatc taaacgagat tgaccaggtt tttggttgat accagctgtg ataacaacga
      961 tatcagcatc tttacattct tcatagccac ctgaccatac agccacattt tcatcgcccc
     1021 aagccatacc atctaaaagg tccaatgctt ccccttctgc tttttcttga ttcacatcga
     1081 tcaatactaa ttcattagag attccttggt tgatcattgc ataagcgata cttgttccaa
     1141 caaaacctgt accgacgatt actactttac gacttgtttt tttcatgaat aaacagctcc
     1201 ttttcataac tatttgttac tttcatgaaa agtatagtct tgtttattca ttttatcaac
     1261 atgttaatta taatagttgt tattataatt tttgatggaa acgaattcct gaatacgcat
     1321 aacagataaa tgacaaatgt ttacacctat atcccctgat aatacaacca aaagtttaga
     1381 actgaaaata aatccaaaaa aattcgctgc aacgatataa taagacagca atcgcaccta
     1441 aaacaatttt ccttcatatg ctttagtgaa agattttctt cttaatgttt atttctccat
     1501 tcattgggag tacatgttga atacttcttg aataatttga ataaagaagt cgtttgttta
     1561 tatccgatac gattagcgat ttcttccaac gaaaaactcg tttcttgaat cagttccaac
     1621 gcaaagtcga attttttctt tagaaccagt tcttgaaagg aagcatcaaa atacattttc
     1681 aatttattac taagatagac cggattgtag ccaaaatgag ctgctgtttc ttttaacgta
     1741 caagaagtaa aatgcttatc gatataatct aatactctaa gcatctcatt ttctgcgcat
     1801 ccatctgctg atgaaaagct ttgttgttct aataaatgag tcaattcgat cagtaatagt
     1861 gatgtataga gatttaacga acggatctta tgactgtatg tatcaaaata aatcaacagc
     1921 atattttcaa tcaaatgcgt caaacgactt gtttttccgc cgcgaaaatg aatgaattgt
     1981 gtatgttccc ctttttcttt tgatgcattt atcaaaaaag aagaaagtag attatttgtg
     2041 cttgccaaat gattcaatat ctctgtagat aaggtttctt cttttaataa gatgttgatt
     2101 aaaatatcct cttgccctgt gtattcgatt ctttgagtag cttctttatc aatgatcacc
     2161 acgtcacttt cttccatctc tatcctttct ccattaataa tttgtaaaca tgtaccgcta
     2221 tacatataat tgatttcaac aaaatcatgt gtatgctcgg gaacttccgc atatcgacgg
     2281 tgtttactga tatgaatatt tccctgttga aatagatccc ctcggtggat ttttggcggt
     2341 gtctgaagat ggtaagctaa gtttaaatca ttgatattca ttccttgttc acgctgcaga
     2401 gtttcgattg attcttcgtt acgcaaaaaa aatgtctaat tcagctagtt ccaattgact
     2461 ccctcctttt tgtaagcggt ttctttcgct tattagttca cgctgtactg ctattatatg
     2521 caaagctatt tttggtttca taaagacttt ctatggttgg gacatgaact tcctgtgatt
     2581 ccgctattga gagaataacc acttcatact atatttttga taagcgctta caaaaatgaa
     2641 aggaacatgc ttatgaataa aaagatgatg aaattttctc tgctgtcagt ctctcttatt
     2701 ttgacttctg ctggtgcagt cacgtctgca attcccagta tgtcagctac ttatgccacg
     2761 cgttctttat ctgctgttga attattaacg actgctccct cttttatggt cactattttt
     2821 gttttgatta gtagttttat tgctaggaaa atcggacaaa aacaaactgt gatcatcgga
     2881 ttagtgattg catcgatttg tggtatcctc cccctcttct cacaaagtta cagtattgtc
     2941 ttagcttcaa gagctggact aggtattggc tttggattga tcaattcttt ggcagtcagc
     3001 atgattgctg ccttttatca cggagatgaa cgagcctcta tgattggttt tcaaagtgca
     3061 tttcaaggat ttggtgcagc tttaatgaca tttgttgcag gacagctagt acagttcggt
     3121 tggcaatata cctttttagt ctacgccatc agcttaccta ttcttgtctt gtttgttttg
     3181 tttgttccta cacctgaaac aactgaactt caatcgcatg gcacagcccc aacaagacaa
     3241 gtaatcaaaa cgaatcctaa aatgattagt tatggtattt ttctcttgct ggtcatcgtg
     3301 gtatataatg ctgtatcgat taaattagcc acagtcctga cgacttattc tctaggaaac
     3361 gaaactaatg cagcgaatat cttaacaatc atgcaattgg caagtatggc tgccggagtt
     3421 tgttttggct ggcttcagac aaaagcacat cgctatatgc ttacactttc tttactactg
     3481 atgggaatcg gctttgtatt gattgccact gtgataaacc tgtatgtgat tgggtttggt
     3541 gcgatcctaa caggaatttc tttttcgtta ttcattcctt acttattcaa ccaagtaagt
     3601 atccaagcgc ccaagtacgc ggagagtttc aatacttcaa tcttattggt tggcgctaat
     3661 ttaggtactt tcattgcacc atatggtttg ctctttctct cttttgcttc tcttgggagt
     3721 aaaacacttg ctgttttcgt caatggcagt atcttgctgt tgattagcgc gcttattagc
     3781 cttctagtag tacacaggac gaatagaaag acagtgataa gagtagatga agaaataaaa
     3841 gaacaattga caacaaaata aatttgatgg aggtcgtgta aaatgacaaa tgaaaaacat
     3901 aaagatagat ggctttggta tcctggagac tttgagatcc gccatggact gctacaaaat
     3961 ttccaacggg aagaacgagg ttttgactgg ccagcgtact ggtacatgga tgattgccat
     4021 cggaacgtca agtttaagag atattacttt cttgatcaac cttccatgtt taaagtgacg
     4081 atccaaggag ttgggtatgt agaaattaat ggacaaaaac atccatgtgg aaaatggctg
     4141 acttgtccag ctggtaaagc aaaaattcgt atctttgtag gacatacatc agggctacca
     4201 gcgatgttta tcgagggaga cgaagtaaaa tcagatattg gctggacagc aagtaatttt
     4261 attgaagaat atccagctgg ctggagtccg ctttatgtcg atatcgcaaa agatccaaat
     4321 caaatctatt atcaaaaaga atgcgtccat ccagtcactg aaacggagat tggcggagga
     4381 atcttattcg attttgggcg agcagtgaat ggctgtgtag aactaatatc tctggatgac
     4441 catatcgaat ctatcactct atgttatgga gagtccgatg tagaagctat ggatatcgat
     4501 tattgctact ataagcaaat aggaatgaca agaaaaatga aagctcgtaa acgcgctttt
     4561 cgctatttgt ttataccaga ggtttctact ggaatggttc aacttcaagt ttttcatgaa
     4621 tttttatctc tttcgaaaca agctgctttt tcaagcgatt cttctttact gaatcagatc
     4681 tggtcagtat cagaagaagc cttcaagtta tgtagtgatt tgttttttat cgacggtatc
     4741 aaacgagatc gctggatttg gtctggagat gcgtatcaat cattcatgat caatcaatat
     4801 cttttttttg acgaggaaat caataaacga acgatattag cattaagagg acaaaatgaa
     4861 atcaagcagc atatgaatac tatcgttgac tattcgatct tgtggttgat tggtatcgaa
     4921 aaccactata tgatgacgaa agactatgaa tttttacgca tcgtctaccc taagatgcaa
     4981 tcattaatga gatacttgat ggagcaaacg aatgaattag ggtttattta tgggagagca
     5041 aaagattgga tatttattga ttggtctgaa atggataaag aaggaacgct tgctgcagaa
     5101 caagtcttat tactgaaagc ctatcattcc atgatcactt gcggagatat cttagctgaa
     5161 acatatggag agaacgaaaa ttatcatact catctctatc aagaaaaata tcaactattg
     5221 aaacaaaacc tatttgacta cttttgggat gaagaacttg gcgcttttat cgattgctat
     5281 gaatctggga gaagaaatgt gacgcgtcat gcgaatattt tcgctgtttt attcgatttt
     5341 gctaacaaag accaacagca atcgatctta aagaatgtct tattgaatga cactatcaca
     5401 cagattacta ctccatattt caaattctat gaacaagatg ctttatgcaa actcggacag
     5461 acgaatatcg tctaccaaac gattcttgat tattggggag ggatgttatc tcatgatgcg
     5521 gtaactttct gggaagaata cgaccctact caaacaggaa atgaaaagta cagtatgtat
     5581 ggagatcctt atggaaaaag cttatgccat gcatggggag caagcccaat ctatctttta
     5641 ggacgctatt ttatcggttt gtatccaaca aagccaggtt atgaagaatt tatgatacaa
     5701 cctaatctag attcatttga aacactgaat tgtacgctac ctatcaaaga tggaaagatt
     5761 tcgcttgaaa aaacaaaaga taagatcgtg attacttctg atagaacagg cggaacagtg
     5821 aaaatgaatg gtaaaagtta tcctcttcta gcaaaacagc cacttgtctt aacaagctat
     5881 tcaaaaataa atgcataaaa caaaaaactg cttgcgacat atcagaagac tttaaatctt
     5941 cttctgatat tgtccaagca atttttattt tgaagtttgc ttaattctgt tctttagaaa
     6001 caatgtttgt tctttttccg gcaaaaagaa agctagataa tacgacaagt aaaaagaaag
     6061 cgatgattcc aactgtcaag gctatttgac cgtattctcc tagtttccca agtacaagtc
     6121 ctggtactag ataatcagta tcggcaaatg tagaagaagc gattttaaga tctcctaata
     6181 ctggaaggat aaataatggc agccaactaa tcaataagct attgaaagca gaacctaata
     6241 ccgcccctct tctaccgcca gtcgagttgc cgaagacacc agaagcagct ccgcagaaga
     6301 aatgtcctac taccccagga atgataactg tagtttgcaa gcctatcata gcaaacatgg
     6361 atagtgttcc tacaaagaag ctaacaaaaa agccaattaa tacagcatta ggtgcatatg
     6421 gaaaaacgat aggaacatct aatgctggtt tagaatcggg tacgagtttt ttagcaatcc
     6481 cttggaatgc tggaacgatt tctgccaaca ccattcgaac cccttgaagg acgataacaa
     6541 acccagctgt gaatgttcca gcttggatca gtgcaaagat tacagcgttg tctccattgc
     6601 ttagttctct ttcaacaaat ccaggaccag ctaacaaagc aagtgcgaca tatacgatcg
     6661 acatcaagag catgatcgat acagtagaat ctcgcaaaaa gctgaaactt ttaggaatct
     6721 ttatgtcctc tgtagaacgg tctttgtttc caaaaaactt tccgatccat ccactagaag
     6781 cataaccgat atttccggta tgccctaacg caacttgatt gtttccagtg atgttcttca
     6841 taaacggttg acaaagagca ggcgtaatag ttaatagtgt tccttcgaag accccgccta
     6901 ataagatcat ccacaaattt gcagtaaacc ccgcgcttac taaaatgact gctgttagac
     6961 aagaaacata aagcattgct tgacctgtaa gaaaaatgaa tttatatctt gtgaatctag
     7021 caagtaaaat attcacgatc atccctacta gcatgatcaa agcagtaggt gtaccgtatt
     7081 gttgcaaagc aatcgccact actgcttcat tgctaggaac tactccttga gtatgcagag
     7141 ctgtttggaa catcgaagca aaaggggtta gtgaattact gattaaacca gcaccgccga
     7201 tcagtactag gaatccgaca aatgttttga tagttccttg gatcacttct gaaaatttct
     7261 tcttctgaag aacgagacca agcagtgcaa tcaaagaaac gaggatggag ggctgactga
     7321 cgattgacac aaacaaattc aacaatttat ccattgatag ttcctccctt gaatcatttt
     7381 tattcttttg acaagatctt cgttactttt tcttttactt cttcgctgtc aatgatcgat
     7441 tgcaaaggta cgagcttttc atcaggtata gaagttagcg tatctttcaa agaatgctca
     7501 atgaagaaat aatcagccat atcgctagat acacttccaa catccgtatg acttacttca
     7561 aactcgtcca catctgctcc aagatctgca agtgcttgtt tgatgttcat ctctaccata
     7621 aaacttgttc ctaatccact ttgacatact gcaaaaattt tcatatcatt cgtctccttt
     7681 tcttatgatc gctaatattt cttcttttgt tgatgcgtgt aataaatctt gaagattttg
     7741 tttattagaa agtagctttg ttaaatttgc caaagcccga agatgtgcct cattatcgat
     7801 agcagctaaa cagatgaaca aactaatggg atgggctgga ttatcaagca gatccacagg
     7861 ttgttgaagt ttcagcaaag acatccctac ttcgtttacg ccatcttctg gacgagcatg
     7921 aggtaatgcg atgtgatcgc caatatggat aaacgcacca tactgctcca cgcgctcgat
     7981 catagctttg atataacttt cttctatttt attttctgat aatagcggct gtgcggcaaa
     8041 tgtgattgct tcttgccagt tttttggttg atccgataat tggatcattt cttctgttaa
     8101 taattcagat agcataggac gttgatcctc cttcttttct aaaagtttgt tcattttacg
     8161 atttaatact tgatagagct tttcttcgtc tacgccttct tttaattcga tataaggcag
     8221 tagcgccgac aacaattctt gggcagaagg cattgaaaat cctggtatca gccaatctct
     8281 ttgtacttga ttgatcagct gatttttttc caagtcagtc atgattggat gcagaacata
     8341 gagagacttt ttcttatcag taagtgtgag aggtacagta gaaaaaataa tatcgtaaga
     8401 agtttcgtca acctcgtgta agcggtcagc tgaattagtc tctgtaaaaa agatcatcgg
     8461 gaataattgc tgaagttcac tttttaagat caatgaagag ctaatgccac ttggacagac
     8521 gatcacggct cttatttttc tatttctttc ttctgcgtcc gcttttctga tctctccgcc
     8581 aaatagtata gtgaaaaaac caatctcttc ttcagggatg ctcttcccta ttcgtctttc
     8641 taacggaagt aatgcttttc ttgtcatttc gtagatggaa gcatattgat gcttaatttg
     8701 atcgatcatc acattaggta aataaaaaga ataggaaata cgaaaatagg cagggaccaa
     8761 gtgataaaag acattcatca ataattcacg aaacgaagca aattctacgg ccatcaggaa
     8821 ttccatgcga tggatgactt ctgaagaaca ttccagcaaa aaatcaagtg caggctcctg
     8881 gacatctcct tcaacgatcg atgctaagca aagagagaaa aatataatct ccgaattttg
     8941 atgttcggaa tagccaaggc cagtcaatac ttgtcgacta atgtcagtga ctgcaagaga
     9001 agccaacagt tcagcatctt ttaccagttc catggatgtt tttccaattc gaatggttgt
     9061 caaactcaaa taataagcta attcatccat tcgatttggt gcgaatcgaa gatctgatag
     9121 tgaaatcgtt ttatctagca aatctctaac tttggcgtaa taatcaatgg aaagggttgc
     9181 ggttaaatac gtgagcccaa atcttccagc aagtgactgc atcaaacgag cagtgcgatt
     9241 ttttgcttct cttcttaata ttgcttctga ccctagcagc ctaaaaccag ttttcctagt
     9301 atacgacagc tgacaatctt ccacttctaa ttcatttctt atctttttga tatctgacag
     9361 gactgtccct ttagaaacct gaagaaaatc ttgaaaatga aaaacagaaa cagacgttgc
     9421 agtcgaataa accatcaaat aaagaagatc tcttcgctct tgttcggtat acacagcttc
     9481 aaatcgagta atatccaaaa atttttctgc ccagtcgatt gctcggaatt tctttatacg
     9541 gatacgggta tcatccacat attcggcaaa aggagttaat tgtttgatcg cctcgcttgt
     9601 atttacttcg atgatctctg ctgctttttc tagactgatt tctcttggtg ttttcaggag
     9661 ataactaatt ttgtagttca cagcgctgtt cacctcctac ttatattcta gtatggtagc
     9721 gcttttaaat cgaggggtgt ttggtacaac ttacagttgg atttttttta cgactgtacc
     9781 gaacgatttc tatcgaatgg atacagaaaa aaagcaggtg accaaatgtc tcctgctttt
     9841 atgaattgat ttcgtccgat caatgatgat catgtttatt ttttgaggtc agttcttcaa
     9901 atttttcttt gacatcttca accgcttttt cgatatcttt ttcaaagtga ttgggctcat
     9961 taggctttgt ttcctcttct acagaatttg aaccagataa agctgtttct ttattttctg
    10021 tttcaccaga gtttgtgcca gctaatgatt cttctttgtc ttctgtttca ccagaattag
    10081 ttcctgctaa agccacttct tctgttttag gggccattga acctcttaat cgtttgtgtt
    10141 tttcaccgtc ataatttgtc atgttggttc ctcctagtag ttctatactt tttcatcgta
    10201 caataagaca gtttatgcgt caaaaaacaa acccgattct tacaaatatg tcatattttt
    10261 ccttctgatt cttgaatgct tttgattcct tcttccaaaa ttttcatttg taagattgtt
    10321 tcttcatctt tgatgacttt ttctgcgcca ttaactaatg tctcgtaaat cccttcgtag
    10381 acacggctgt aatcaccaac ctcggaaatt actttttctt catgataact cccttgttca
    10441 tctatatagg tcaatacacc ataatgttcg ggcaaatcga taccaaagtc ttcatgatca
    10501 ggcatgtaaa acattttcag atgttcttct tgtcgatctt ttgtttgttt cacgaacatc
    10561 cctttttttc cgtaaacgac gaaacttgga cgttctttga tgcggaaata actggatttt
    10621 atcgaaattt tcatatttcc atagaaaaaa tccagatcga agtaatcatt cattcgtcct
    10681 ttccctaaaa gttgacgtac atcatagctg atatcatctg gttcgccgaa ataagaaaga
    10741 acttgatcta ctgtatgaca agcatgtcca tataaatagc tgttcgcagt agaaaattca
    10801 tgcgtatttt ctggtacttc cggtctgaaa taatcataat gcatttctac ttctaaaata
    10861 tcccctaatt ttccgctttc gattactttt tggactgtca agaagtcaga atcgaaccgt
    10921 ctgttttggt aacattggat aaatagacct ttttcttttg ctaatgcaaa taactcttct
    10981 gcttcctttg acgtttctgc aaaaggtttc tctactagta cattcttacc atgttcgagt
    11041 gccatttttc catattcata atgaaattga ctaggtgtag tgatgacaac taattggatc
    11101 tcaggatcat cccaaagatc agacagctga tcagtatatt cgatgtcttc gaatgttttc
    11161 cacactggtt cagatgggga aaaaattttt ttgatattga ttttgttcgt aagttttctt
    11221 gaaaaaggca aatgatagcg atttgtactc tttccattgc caagataagc gattgtaagc
    11281 atgagtggtg cctcctttta ttgatgtttt cagtatatcc tagaaaagcg cagaaagtat
    11341 ttataatagc tattctggta aaaaagttca gaaaaacact tgttatcatt cggtttggtg
    11401 aaattaatgt tcaaagtaag gataaaaatc aaaaagatag tagcggaacc aagtgtttat
    11461 cagtaagata gaatttggag gtcagatcat gcttattgaa gaaaaactaa caaaacaaga
    11521 gttgtttacc acgacagaga aacggattgt cgattacatc cgtaggaata tcgaagctgc
    11581 agtctacatg acgattgaag aattagcaaa agctacgtat acttcccatt cagctatcat
    11641 tcgtttatgc aaaaaaatgg gattcagcgg atttaaagaa ttccgtttcg agctggggcg
    11701 ggaagttcat caattgattg ctcagttcaa tcaaacgatt gatcctaatt ttcctttttc
    11761 aagtgaagat agcactcagc aaattgcaaa gaaaatggcg gatttatcga tccagtcaat
    11821 aaaaaaagca caattgcaga ttgaacatga agatttagat caaatcggta aagtattgaa
    11881 caaggcacag cgaatctttc tttttgcaaa aggtgattct caaatcacgg caaggaaatt
    11941 ccagaataaa atggtcaagt tgaataagtt tttgatcatg gctgaagaat acagtgattc
    12001 ttcttggaat gcagccaatt tgactagcga ggattgcgcc atttttatta gctacagcgg
    12061 tcgcatccat cattatgaac gaataatgac ctatttgaag catgtagagg cccctacctt
    12121 gctgattacc ggaaatcagc attcagaaat ggccaaacag gctgatatgt gtcttgttat
    12181 atcgcaggag gaatatgatt ttgctaaagt agcgactttc tcctctcaaa ttgccttcga
    12241 ttacgtatta aatacgttat tctcagtgat atattctcaa aattttgaag agaatatccg
    12301 caacttgaaa aacaaacaag aattgatgca aaaaggcctt cttaaagaat atcggaaaac
    12361 ttaagaaaag atcctcattt agtaataaga aagatagtgt ttgataggct ctctttgata
    12421 tcatcataaa aacaggctga agttttcttt ttcaaacctc agcctgtttg tttacggaac
    12481 gattcgaata gatatattat agaaatgagg aatagctaac ttctgtttct tattttgaac
    12541 gattgacgaa aaagatatat aagaaggagg aaaatcagta tacatgcagc taacaatcca
    12601 tacaggaaaa atcgtttgtt ctcggatact ttctgtactt ctttttgaac ttcttttgca
    12661 tgataaggaa cccggtgccc tctgacaagt aagcgatgac tgttgaccat ataaggtgta
    12721 caagttagga gtgtcacgaa atcctgccct ttttcgattc tcagatcttt tgtatcagtc
    12781 ggttctaccg tcttgatctg atcgacttga taagcgagtg tccgtccatt gatttcgata
    12841 tagaaagaat cacctaaagt cagtttcggt aagtctgtga acaactttgc ttgaggcagt
    12901 cctcgatggc tggaaataac ggcatgtgta ttttctcccc ccgtaggata agacgttcct
    12961 tccaataatg aggcaccttt ttctagcagc acagtattcg tttcatcaaa aattggcaaa
    13021 tggacattga tctcagggat cgtcaaaata ccgatcgtat gtttctcaaa ataggttcta
    13081 tcctttttcg caggttcttt tttcttagta aagggatctg cacctggatt tccaccttct
    13141 tttgctaatt gctggttttt ctttgtcatg ttttcttgga ttttagccat gacttcttca
    13201 ttttctttga ccgcttgttg ctgataatga gagatgatct gctgatccag ataattattc
    13261 agcgcgtcgc taacaaatgg ataagctagt atccctattc ctgagagaag caagaaaagc
    13321 atcagcagat ctagaagttt ccttttcttt ggtttcattt gtgttctcct tgtgatgact
    13381 gtttgataat aaaagaaaaa ggagcatttg cgtaatccgc tgctcctttt tccaattctt
    13441 gcaaaaagac taaatctgac tgtgcttgcg tctagcaaag tacagtccag caatcagtag
    13501 aaggactact cctgcaccga tatacacata gattccctta ccgcctgttg aaggaagtgt
    13561 acctttgtgt ttatttggta ttttttcagg agaaatcaac tgtcctgctg ttacataaga
    13621 ttgttcatcg atagtaaatg ctacacggtt tgttaatgga acatattttt ctggcgcttt
    13681 cgtttcttcc agatagtacg tgccatattt tagacctgtc acatcgatta aaccgttact
    13741 tgtggttgta aaaaccgttg ctgattcttt cgccgatacc cagctgacgg cttttgtgga
    13801 tgggtcgatc gataaatatt tcgctgtgtc actatcttga tcacgaacga cgaattctgc
    13861 tccagcaagt gtttggtctg atgtaacgtc accatctact ttaacaaatc gtttgccccc
    13921 agtaacgaca tcgactgacg gtggtgtttg atcatttgta tgcccgttat cgacatttgc
    13981 ttggttagaa aatcctttgg ttggatctgc tttttcgttc aaatgcatat agtaaacgaa
    14041 tttcaatgta ccgccaggag ttaatgaagg aatataattc ggatcaactg aaaccgtgaa
    14101 tccgtctgtt tgctcagtga cagaataatt tactgggtcg atttctttat ttccatcata
    14161 taaggcataa gcatacgttc ctgaagaatc attatcaaat gttaaagcag cgtcatgagt
    14221 atcgataagt ttgaatgttg tgtacttgtt ttgcgttcct tctttatcag cgatacctaa
    14281 tgggatattg acagaaatct catattgaat tttttcaccg attgcgacat cttttccatt
    14341 caattgaggt gttgttttat caactttaga cgtatcattt ttgatggtct tttctactgg
    14401 tgtttggcta tttgccagga tctcaaaagg cgtgattgtt tgcttttcaa tcatttccgc
    14461 attatcagga gcttttactt cttctaaatt atattttcca acttctaaat gattaacgat
    14521 caactggcct ttttcaatag atgcctcggc aaagtcattg ttgccgatgt cataagaatg
    14581 accagtaatg aaatgtttgg ctttggtttg atcagttgtc caagtgtaca atccatctgt
    14641 gacactttgg atgtattttt tgacgcttgg tgttccttct tctttagaaa taataaattc
    14701 tgctccattt agtgcttcgt tttcggcagt accgattttt gtaactttca acgttccatc
    14761 attaccgact gtatttttag ggtagagatg gatagtatct agttcttctg tcccgtattt
    14821 ataagagcca tctgcttgtt tgatcatctc atatacaggg aaagctaaaa ccatgtttgc
    14881 ggcagctgac actccgtctt ttggttcttc tttgatcgtg tagactgcat ctttcccatt
    14941 ttgtttttta ggtaaagata aagtgacatt tccatcagca tctgtcgttc ctgaagcaac
    15001 tggtgtacca ggagtcaaag actggactgc ttgttttgct gcatccacgg acgctccttt
    15061 atctcgttgc gcataaaatt cttgagtgac gttataaact gaaaatgaaa tatcggctaa
    15121 tccttggtat tgatcgaatt cgctcatttc tttcccgctg ttttggatta aaggatcggg
    15181 taaatcagtc attttctttt tatgaaggat gacttgagct gctgtctcct ctgcaaacac
    15241 tttttttgcg ccgaagctgt ttgtgagtaa tggtaaaata aggaaaagga ctcctaacat
    15301 aacgtttatt tttttatgat ttttcatttt ttcactcctg ttcttttttt gataaacaac
    15361 cagattgcaa agcttgcgaa taccatacca acaacgccta aaagtgtatt tttcgtttcg
    15421 tttgtttttg gaagaaatgg acggctattt tcttttttat tgactacttt caatatactg
    15481 tccgcctctt tatcctcaga aagaaaagtt gaaatggtaa acggaatttc tgtttcactt
    15541 aaaatataac cagaaggtgc ttcgatctct tttaatcgat aacttcctgt tttcaacccg
    15601 gaaattgaaa aagcgccatt tttatcggaa atcaggtgaa gcgcatctga ttcgttcttt
    15661 gtccacttgt atccgttggc tgtttgctgg aggtaatttc ctgctttgtt tttaataagg
    15721 aatactgcgc cagctaatcg tttatctggt ttatttgtct cgattttgac aaattgctta
    15781 ctccctgtat atacagagac tttctttgta ctgacaccaa aaccaaaatc cagtgttccg
    15841 ttgttgttga tttccttgtt cgcctgtgct gtactgctta gctgcatctg ataagaaatg
    15901 gtcagttttt ttccagcata tttttccaac gaagggatcg aaaataaaac atcaaatccg
    15961 tgaggtttct tgtgtatgac aaagccttct gtcagctttt ctccattcga tgaaatcgtt
    16021 aaactattag gtaaaaacgt taatgcttca tccgcactat ctgacaattc gaacttttga
    16081 tagtcaagga tatttacagg aattgtcgta tgcaaggaat aagtgatttt ttcaccaatc
    16141 gtaaaatcat ttctaggctc ttctaatacc ttctcaaaag gtggatcagg gtagtcgttt
    16201 tcttcatttt taggatacaa atggatagtt gaaagttttt gattgttttg tccatgtaca
    16261 ggaagaacaa ctaccatatt ctctgccttt tctttgacga cttctggtac tttggattca
    16321 atgaaaagat acgctttatc tcttttttct tgatctttag cggccagaga aaaagaagcg
    16381 ataccatctt cgttgttaag agttgtagtt gttgcttctg cggtaaacat accggaagac
    16441 gcaccgtttt ttgcgatctc tgcttgtgct tcttctaccg ttttgccctt ttcccgtaga
    16501 tggtaaaaag aatctgtgac atcataaact tggaatgtga caccatttaa tcctcgatac
    16561 gtttgtaata aagctttttc ttgtccgtca tttggatgat ttttcggcag ttgcccgttt
    16621 gggaaaagca atttgtgtaa agtaatctga accatttctg tttctgtttc tgttgctacc
    16681 tgagtaaaag ctggtttaaa tagtaacaag aagagacaca tactaagcca accaagtttt
    16741 ttcatattta gctgccccct ctttcttttt tcaaaaaaag ataccaaata gaaaacgcac
    16801 aaccaatcat ccctactaga tagattccta aacggccaat tcctcccgtt tcaggtaatg
    16861 gtacttttgc ctgattcgtg atgtctaaag aaatctggtt gtttttggct cctggtgaca
    16921 gaacagattc atgatcttgt ccatccactt gaattgaccc atcttcgtgt ataactatag
    16981 taactggctc ttttagacct tggtatcctt ctggtgtaaa agtttcggtc agcgtataag
    17041 ttccaggttt taaattttcg aatagaaagg tatctgtttc ttgtccatct tttggcgatt
    17101 ctaagtccat ttctggtccc tgcagtctga attttgctcc ttttaatgtc tgtccgttgt
    17161 cttctttttt gtgtaccgtt agatcaaacg cttttagtcg attttgatga gtgagcgtcc
    17221 atacattatt tgtcatctcg gtattttttc ccgtgtaaat gatttttcca tatttatcga
    17281 tttggaattc ataatttccc ggcgaagtat catatccggc aggaccagct gtttcaacga
    17341 ctcgatattt accaggtgtg agtgaatcaa aactggcaag acctttttca ttcgttgttt
    17401 ggctgtcaat tggctgataa gtaccatttg cttctttttt ctgtagggaa aatgctgctc
    17461 ctttaagatt tatctctttg tccgtccctt gcatcgcata cttacgtact gctacaggaa
    17521 cttcaacaaa aggattttca atagtcaact ggatcgtatc atcggaagta gtgactgttt
    17581 ttccatcaat ggttaccgta ccatcagaag cgatcttgat ttcccaagta gtctttttgc
    17641 ttaatccatg cccttctgga gcttttgttt ctgtcagcgt ataggtcatt tctttttgca
    17701 atttgacatt ttctggaaga gaataggttc cgtcgccatg atctgttagt aaagtatcaa
    17761 tagaatctcc tgttaatttg aaaacagcgc caataagatc cttttcacct gtagaggaat
    17821 ttttcgttat tttcaagttt aacggtgtga attgtttagt atttttccat gtcattgcat
    17881 ctatttgttg agaatcgtat cctggtacag gtaattcttt gattgtttgg taactgaatg
    17941 cttgaccttg attattgtat tgaggtagtg ataatatctc ttgataactt tctccgctat
    18001 ttgcagataa tttgtcaatg tctgcacgtt cccacgtatt tgttgtatct ttagctggtt
    18061 taatgattcg aatatatccg tttttccaag ctgcagcatt tgtcgtatgt tcccgttgaa
    18121 tctcaaaagt aacttgatct ggacgatcag ctagattgtt gtcaaattct tcccataact
    18181 ttttgatgtg aagactgact ccgggagctt tagcagatgg tataccgaat tcagctaatt
    18241 cattggtatc aatgtttggc tggaaagttg tcctgccgtt catttgatac cagaaatttg
    18301 gatggaagtc ctcattttct gtttggattc tcacttggta atggatttgg atttcttggt
    18361 cttttcctaa atagatctga ttgctcttga tggtatttcc ttctatggaa atagatggag
    18421 tgaccgttgt aggacttgtc cccacactct tgactgaaag tgtaccaggc tgataaatga
    18481 atggttctgc gattggatca tttatttgtc cattgcttac agttgctgag atctgtacag
    18541 cttttttggc taggtattca gagatatctg ttgcatgatc agctgattca tagtaaagat
    18601 cgcctttttc atctgatgaa accatttgtc gcatacgaga ctctacttct gcttttgaga
    18661 gaccagctgc cggatcgctt tgaagttgga tgccaagacc atgtatttcg attcctcgtt
    18721 ctttgagtgc catcgcttct ccgatggttg cgataaacgt actgtcgatt cttctggata
    18781 gattgttttg gtcaggtgca tcatagattc ttgagattag agaagtattt cccggccgat
    18841 cttgcgtatt agaaaactga gttccgtaat aattgctgct tgattgtgcg tgtacccgct
    18901 gtactttata ggaaaatgtt ggtacaccat ccgtcagcaa aacgatcact tttttatgtc
    18961 catttggaac ggatagcatg cttcctgcat ctcttagtgc tttttgtgta aaagtaccac
    19021 catttgtccg tgaaggtgta atggatttta cttgattttt cactgaatca aatgaaccca
    19081 tctgtactgc accgttactg tagctatatc cttcgcttga gtagccgaca tatcccatat
    19141 tgattttgtc tgtgataccg ctatctgcta aagtatcgac aaaacgatcg acaccaatct
    19201 ttacttcacc gatccgatta ttgtcgttca tacttcctga ccagtctacg accaatacga
    19261 gatcaagagg cgtgatatct ttttgtacat ttccacgagc attcaaataa acatcaaaca
    19321 atccgggtgt acttgtttcc tttgcatact ttcgtatcgc atagtctgct tgattaccgg
    19381 ttcctccata ttcgatatac gaatgggtcc gatcattagg atctccattc caagaattta
    19441 taccatccca ttgatttgtt ccgtttttat tgccttgatg atcaagaaca tttgtatttc
    19501 ctgtaggttg ccaactggct ttaggataag tgccctgatc atctgttgta tattctgtag
    19561 gaatcgtttg attagatggc acactcacag ctggcaagga taatgtaggc aatgtaaatg
    19621 ggctgacaga attttcgttt tcttttgatg attcttcttc tatttcgtct ggctttggta
    19681 ctgacacatc tgtatctttc ttattttctg tctcatcaat aatgtcatat tctttattat
    19741 cgacgatcag cttgtttgcc tggctgtggt aaagtgctgg atcgattgga aaactcagat
    19801 cgatagtttg tttagtgttt gatgagattt ccaacgtaat tttcccgcta tttacctgat
    19861 aagcatagcc atctccttct tttctttcca catcacttaa gtaattttcg actagaccaa
    19921 tttctttttc tatcttttca gcactggtgt tattcgcatt tagcttgatg ttcagtgctt
    19981 tttttccaga ctgatccgaa atgacttcag ctgaaattgt ttctaaaact gttggatcat
    20041 ctgtagtgtc tgctattgca gataatggca caaagtttga taaactcaat attatcagca
    20101 tgaaaataac tttcagtttc ttccctgtgg tcgtcatttt tcccctcctt tttctttttt
    20161 tctgttttga cgtgaatagt gaaaattatt aaaataaaca ggcgttacat aattttattt
    20221 attttaatat gataattatt atacgaaggg cgctaaaggg aatcacacca attttcatac
    20281 caattggttt catttatgaa gttgattcct tcgattaatg agcgtttcat tgaaagaaaa
    20341 gggttgaggg agaaaaacat atgatcaggt tgttggaagg aaactataga aagatggaaa
    20401 aaatcattcg aattctgtcc atttctgaca agtggatgac taaaaaagaa cttgccaaac
    20461 gaattggtag ttcagaatct acatttgtaa ggtatatcga agagatcaaa atccgctggg
    20521 gaacggatgt aacgattcaa tcttcacaaa aattggggta tcgtcttgaa catttgaatg
    20581 taccagctta tttaaatgtt ctgatggaaa tgtcgcaaag ttctacaaac agtcgtttat
    20641 taaatgagat tattgtcaat ccgggtaaaa cgattgagta ttattgtgaa aaaatttcca
    20701 tcagccggtc ttcttttaca cgtaagttga aacaatgcaa tcaaatttta gaaagctatt
    20761 tattaaaagt gatcgttgac caaggttttc agttaactag ccttaaaagt gagctccagt
    20821 tgcgtatctt tgttaccttt ttctttttaa gttactacgg aagacgcacg ttgccatttc
    20881 caatcgaaaa aaagaagatc aaagaaatta tggtcaagaa taattgtcag atgaatttgt
    20941 ttcaggaaga aagcccatat gagcaatctt tctttattat gtattttgtt gtgcatctca
    21001 tgagagaatc ccaaggaact ccatttgaat ggaaagctgg agactcttat gtacagttga
    21061 taaatatcga taaaaaagac ttcttaatct tgaaaaatct cttaggaaaa ttgaagtaca
    21121 gcagctatat aaaagtagtg aactattttg tcaatacgat tttttcagaa ttttcggaga
    21181 aagaaaaaaa tgaaatcagt gagcagatac gaaaaaatat gtcctactgt tcgttttcat
    21241 ccgctgattg caaagaaaaa aacgaagaat atgttgttca attattaacg caaatttatt
    21301 ttttctcccg tattttccca tacgatacga ctcatttgac gatccgtgca acttactttg
    21361 cacagcagat gcaacaccgc cagatacgtc tatatgctgg atttcgtgca gaacttcaat
    21421 tttattccac tctactgaat cagaatctga tttattattt atctagctat atcttttgga
    21481 tgcttatcgg taatcctgaa attcaccatt atacttctga taaaaaaata ctgattatca
    21541 gcgacttaag tcttcgacat agtcaatata ttgaagaata tatcagtgat attcttgctg
    21601 tgcataaaat tcactctgag acaactgcta ttactgaaga tcagctgtcg aaatacaatc
    21661 ttctcgagta tgatctgatc gttacgaatc aacccatttt gaatgcccat gttcctcata
    21721 tattgattga tgatagtgtg tcattcgcaa atgaagaaga actcatccgc ttattcgagt
    21781 tatgaataat caaaaaacag ctatgtataa accaccctga taaaaatttc aggacaaata
    21841 cacatagctg tttcttttta ttgtattcct tcagcaatcc aacgagattt atagcgaaag
    21901 tcttttaaca actcttcttt tgtccaatta tttgcaatga gatccgtcaa agtgttcatt
    21961 acgagggtca tggaaaagta taggaaacta actgcttctt ccgacgattg aatggagaaa
    22021 tgattgattc ttaataattc cagccatttt tcgttcattg gagaatcagc cgatagttct
    22081 ctacgtttgg ttgtctgctg atcatttcgc tgggttaaca agtataacct ttgccaatag
    22141 atactttcgt gatctaagtc gcagcaccac tctaaatatt tttcgatccc caaaaagaaa
    22201 tgttgtttgt tttcgctaat gaattttaag atatcttgat ggactatgtg agaactgctt
    22261 ttgatcgtat acaaataagc atcttccaaa tcttgaaaat atttataaaa agcaccgcgc
    22321 gacatcccca tcttgtcaac gatttctgag actttgactt ggctgatgtg ctgtgagcaa
    22381 aatatctcca gtaataggtc atcgattctc tgtttctttg cgttcgataa attgaaaaaa
    22441 gtttcttttg gcatccacat ctctccttta tctctgatta tacgtggata gcccttcatt
    22501 gtcatgaagg attcattcct tctttttctc ttcttctgtc aagtacccat cttccattcg
    22561 ataaacgcga tcactccatt gaatcatacg gacatcatga gtaaccataa tagtcgcttt
    22621 ttgtttctca tgagcttctt taactagtaa tttgacgact tcatatgcat gatctgtatc
    22681 caaactagct gttggttcgt ctgctaagat caagcttggc tcattatata aagcgcgagc
    22741 aattgccact ctctgtcttt ctccaccaga aaggtcttta ggataactat ttttaagtga
    22801 ctggatatct aaagattgca gcaaggaatc gatttttgtt gaatcttctt tttttgaagt
    22861 aacttggtca actagttcaa attgttcctt taccgttaaa aaaggaatca aattagagct
    22921 ttggagaata aatcctattt ctttaaagcg taaatctgct cgtttttttt ctggaagctc
    22981 agttagtgag tgaccgttga tcactatttc tccggaagtc ggtgtttgca atcctcctga
    23041 aatggtaagg aaagtacttt tacctgaacc agaaggacca atgatgctga cgaattctcc
    23101 ttcctttaca taaaaatcaa ttgctttcaa agctgagacc tttgtatgac cagaaccaaa
    23161 tgttttggta acttttttca tcgttaatat ttttttcatt tttattaacc tcctattgct
    23221 gtaattgggt ctactttggt tatcgtacgt atcgaaaaca atccgcctag cattgcgaca
    23281 atgatcaaaa tcccactata tagcaaccat tgtgaccaaa aaacagcaaa aggcatcgcg
    23341 cttggtaaaa tcagactagt caaataagca aacaaaagag caagcagaac gcctaccacc
    23401 ccaacaataa aggactgtgc tactaaagat ttagcgatga agaaattttt gatcccttgt
    23461 gctttcatta ctccaaaaat agcagtcttc tgaagtgtca tcacgtacat gaaaataccg
    23521 accacggcag caaccactaa gaacaaaaag tatatcatcg catttaaggt catattttgg
    23581 gcagaatagc caggaatact ttcaatgaat gtcccaatag ttaataactg tagacccttc
    23641 gatgcttttt ctttcgaaat tttagctgaa tcctttatta cgatcccatt gatcggctgt
    23701 tcttcttcag aagcaaacgg ctgttgtcca tacttcaaag cagtccacgt atctagggac
    23761 gcatagatga cgggagaaac tgtgtaataa gtttcaggaa agataccgac aatcgtcaaa
    23821 ggatcgtcgt aactgccaat tttgatcgta tctccaattt tatagcctga atcagccaga
    23881 ttttgagaga tgatgatttc attctttgtt ttaaattcat gccctttcgt gagttttggt
    23941 aataaaaatg cttctttcgt tgtgccgaaa actgtaacat tttctttgtt ttttcctttg
    24001 attgctccgc tgtacaatcc gattggtgct ttctctcctc cttcaatgta tttcagatct
    24061 cctcttgtta attgtgaagc tgcaaatact tgatttgcat cctccgacaa aacaatctct
    24121 tgtgcgtccc aatcatcgat tgctttttta aactcttctg aaagtccatt cgctagtcct
    24181 gacaacatga atacgacata cgcaatcaag aacataatac cgacgatcaa gccatatcgt
    24241 aatttagaat agcgaatttc tttgatagct agaaacattt gtttttcctc cctatgatag
    24301 attgatagtg acaaaatgtc acttgttgaa tagtgtaact ctaaagtgac aatttgtcac
    24361 ttgtgttgtt tttttcttat caataaattg tctaagacat ctttcgataa aaccttaaca
    24421 tgagtcataa aaaatttatt taagccttat ttatgctttc catgataaac taaagtgacc
    24481 aaatcaatga ggtgggaaaa tgattaaact attagtagta gaagacgata aagtattatc
    24541 caataatata aaagaaatac tcgctgcaat cggcgagata acacaagttt atgatggtag
    24601 tgaaggactg ttcgagttgg aaagtggagt ttatgatctt gcaatcttgg atttgatgat
    24661 gccggaaatg aatggatatg aagtgttagc aaaaatacgg aaagaaaaaa tacagactcc
    24721 agtattgatt ttgactgcaa aagatagttt ggaagataag attgaaggat ttgaacgagg
    24781 tgcagatgat tatctaacaa aacctttcta tcgagaagaa ctgatcatgc gcgtgaaagc
    24841 attattgaaa cgctcgctag gtctctttga tgaaaatgtt ctgaagtata aggatatcag
    24901 ttgtgattta aaaagcaatg aagtcttgta taaagaacaa gttttgccca tacaaggaaa
    24961 agagtttgaa ttgctcgttt attttttaca aaataaggga atcattttga caaaagagca
    25021 gatatttgac cggatctggg gatttgattc tgatacgacg attacagtta ttgaagttta
    25081 tatgagcaac ttacgaaaac atctgcgaag tactggattg gataaagaaa tcaaaacctt
    25141 acgtaatgtc ggttatatac tgcaaagggt gtgatgcaat gaatggagat attacacgaa
    25201 agcaacgtag tcgctttttt gcgacaaatg tagcagcatt tgcttttatc ttttttcttt
    25261 tgggattcat tactttacag atattaaacc aatctgcata cagagaaaca gatctgtcat
    25321 tagagaaaat aaattttgat tctcgaacag tacaattaga gatacaaaga tatcagcaag
    25381 gcgatccctt tttaaacccg actcaaacgg aacaaaacag aacaaacatg gaacagccat
    25441 taatggaacc atcaaatgac cgttttaaca ctcaagtgat cttgtggtct aaaaatggtg
    25501 agatattgaa taaagcagct attggtgggc gtttcaacca aatcaaagaa ttaaaaatgg
    25561 atacgaaaaa tctgaatacc atccaaaata ttgaattaaa tgaagacagt ggctcatctg
    25621 ccctctcttt ccgttcgatt acgaaaaaag cggagacgaa tcaagatggc gttgcctaca
    25681 tccaaatttt agaaaataca aatcaaatca agaattcatt agcgactttt cgcacgatta
    25741 tgattttgtg tatgattgtt ttttggattc tctctattgg tatcagttat tacttgtcta
    25801 gtctaagcat gcgccctatt attctttctt ggagaagaca aaaagaattt gttgagaatg
    25861 cttctcatga attgcgcaca ccgttaacga ttattcaaaa tagtttggaa catcttttta
    25921 ccaagcctaa tcatactatt attgaagaat ccgaaagcat cgctcaagca ttgtcagaga
    25981 cgaggcgcct cactggattg acttcggatt tgctgacaat cgctcggaat gattctgatc
    26041 agcagctgct ttcaaagcaa atgatcaata cacaagaata tatcgaacaa cttgttaaac
    26101 catttcaaga gatggccatc atagatggaa aagagtttat tctccataac gatgcaacat
    26161 tagaagtacc aattgatgaa aaaaaaatcc atcaagtttt ggttattttg ctggataatg
    26221 cattaaaata tacgaatcca gaagataaga tcattttgga ttcatctgtt aaccataaag
    26281 aatggctgat tcacgtaaag aatactggcc cttctatcag tgaagaagat caagcaagga
    26341 tttttgatcg attttatcgg gaagatcaat ctcgggcgaa agagactggc ggatacgggc
    26401 ttggcttggc aattgcaaaa caggtaatcg aacagcatca tggacagatt tctgtatcca
    26461 attatcttcc acaaggagtc gattttaaag tccaattgcc gttgaagcct aaaaaataat
    26521 gcttatccct aaaaaagggc tgtgacaaaa atcttgtcac agcccttttt tgttgcgaat
    26581 aaacggtgtt ccagaagtag cttcttcgga aataagccga aatttcttta agctgaccac
    26641 ttctgtcaca acctctttag cttacgctta cgatttttta ttgcggtctt caatgtgtac
    26701 atcaccgctt gtgtcaatat ccaattcttc tcggctaaca tcttctgtca ctttttcaac
    26761 atcttcatgt tcttctttat gaatcccaac ttcttcacga ataactgtat gtttgcttac
    26821 atctacttgc tcttctttga ttgggatagt aatcgtgtca tcttccaatt tttcgtctgt
    26881 tccctgtgaa gattgatctg ttactggttt gcgttcgatg acgatttctt cacgtttgac
    26941 cggtacttct accgtttcag tatcgttgac cacatgtttg tggatgtcga cttctccagt
    27001 tgtcacatca tgtgtattta catctaattt ttcttctttt agttgaattg tatcatcatc
    27061 tagatttggt ttgcgatcga tgtcttcagt cttgcttgta cccattgtat cagtctcgcc
    27121 aggtacgtcc atcggatcag ttcctggtgt catattcaat ggatccgttg tattttgaaa
    27181 gcctgtcgtg ccacctacgt ccattggatc tgttccagtg ccagtttcta atccagtccc
    27241 cattgtgctt gctgggctaa cagtatcttg tgtatccatt gtatcttctg ttgtttctgg
    27301 ctgacgatag ccttttactg tgatgacaaa ttttccatca gaaatatctc ctctatattg
    27361 atataaagga tcattttctg gtgtagccgt ctcagaatca taggtatcaa aagaaaaagt
    27421 atcttttatt ttttcccata aagaacggtc ttcttcacca tccatccgat tttcatctgt
    27481 ttcaacatct ataccagaca atccgtcaaa tcctagtcta gatgctgctt catcagtagt
    27541 ataaataacg atatcatctc gttcataccc ttcgttacgc aaccgttcaa ttactgataa
    27601 tgcttcttcc ctcgtactat agcttccgat gaccgttaaa tttttctctt ccatgtaaat
    27661 tcctcctagc aaatagattt atgattggta tattgttact tataaattta ttctatagga
    27721 gacgattgtt ttctacaaat atttagattg gaatttgatt aaaacaattt aattgaaatt
    27781 tgaaaacatt tatgttaatg aaagtcatat tctaataaaa tgtcatttgt ttctatatga
    27841 atttttatga a
//
LOCUS       DARBDV010000029        27423 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000029 DARBDV010000000
VERSION     DARBDV010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.029"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            199..2733
                     /gene="secA"
                     /locus_tag="SCM56_001184"
     CDS             199..2733
                     /gene="secA"
                     /locus_tag="SCM56_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEG1110000.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            2859..3960
                     /gene="prfB"
                     /locus_tag="SCM56_001185"
     CDS             join(2859..2930,2932..3960)
                     /gene="prfB"
                     /locus_tag="SCM56_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEG1110001.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            4065..4751
                     /gene="ftsE"
                     /locus_tag="SCM56_001186"
     CDS             4065..4751
                     /gene="ftsE"
                     /locus_tag="SCM56_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEG1110002.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            4735..5628
                     /locus_tag="SCM56_001187"
     CDS             4735..5628
                     /locus_tag="SCM56_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1110003.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            5780..7228
                     /gene="cls"
                     /locus_tag="SCM56_001188"
     CDS             5780..7228
                     /gene="cls"
                     /locus_tag="SCM56_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEG1110004.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            7565..8422
                     /locus_tag="SCM56_001189"
     CDS             7565..8422
                     /locus_tag="SCM56_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEG1110005.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            8537..9457
                     /gene="pstC"
                     /locus_tag="SCM56_001190"
     CDS             8537..9457
                     /gene="pstC"
                     /locus_tag="SCM56_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEG1110006.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            9457..10341
                     /gene="pstA"
                     /locus_tag="SCM56_001191"
     CDS             9457..10341
                     /gene="pstA"
                     /locus_tag="SCM56_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEG1110007.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            10356..11162
                     /gene="pstB"
                     /locus_tag="SCM56_001192"
     CDS             10356..11162
                     /gene="pstB"
                     /locus_tag="SCM56_001192"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEG1110008.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            11178..11936
                     /locus_tag="SCM56_001193"
     CDS             11178..11936
                     /locus_tag="SCM56_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEG1110009.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            11949..12626
                     /gene="phoU"
                     /locus_tag="SCM56_001194"
     CDS             11949..12626
                     /gene="phoU"
                     /locus_tag="SCM56_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEG1110010.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            12864..13895
                     /locus_tag="SCM56_001195"
     CDS             12864..13895
                     /locus_tag="SCM56_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEG1110011.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            13892..14659
                     /locus_tag="SCM56_001196"
     CDS             13892..14659
                     /locus_tag="SCM56_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEG1110012.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            14759..16327
                     /gene="liaX"
                     /locus_tag="SCM56_001197"
     CDS             14759..16327
                     /gene="liaX"
                     /locus_tag="SCM56_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HEG1110013.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            16624..16944
                     /locus_tag="SCM56_001198"
     CDS             16624..16944
                     /locus_tag="SCM56_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEG1110014.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            16944..17294
                     /locus_tag="SCM56_001199"
     CDS             16944..17294
                     /locus_tag="SCM56_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEG1110015.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            17461..18399
                     /locus_tag="SCM56_001200"
     CDS             17461..18399
                     /locus_tag="SCM56_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEG1110016.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            18414..19244
                     /locus_tag="SCM56_001201"
     CDS             18414..19244
                     /locus_tag="SCM56_001201"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEG1110017.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            19288..20313
                     /locus_tag="SCM56_001202"
     CDS             19288..20313
                     /locus_tag="SCM56_001202"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEG1110018.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            20328..21266
                     /gene="galU"
                     /locus_tag="SCM56_001203"
     CDS             20328..21266
                     /gene="galU"
                     /locus_tag="SCM56_001203"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEG1110019.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            21391..21831
                     /locus_tag="SCM56_001204"
     CDS             21391..21831
                     /locus_tag="SCM56_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEG1110020.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            21834..22436
                     /locus_tag="SCM56_001205"
     CDS             21834..22436
                     /locus_tag="SCM56_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEG1110021.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(22495..23601)
                     /locus_tag="SCM56_001206"
     CDS             complement(22495..23601)
                     /locus_tag="SCM56_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEG1110022.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            23811..24812
                     /gene="ccpA"
                     /locus_tag="SCM56_001207"
     CDS             23811..24812
                     /gene="ccpA"
                     /locus_tag="SCM56_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEG1110023.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(25149..>27423)
                     /locus_tag="SCM56_001208"
     CDS             complement(25149..>27423)
                     /locus_tag="SCM56_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEG1110024.1"
                     /translation="FRVFQSLTVFITVLLLMFAALGIGIGAGYFAYLVEDTQLPTKKA
                     LQTELGNITETSKIVYADNTEISKIQTDLMRTTISSDKISPLLKTAIISTEDEYFDKH
                     QGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQQILTDETTFKRKANEIILSAQVEK
                     YFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAKGIFGVDAKDVTLPQAAYIAGLPQS
                     PITYSPYTNTGALKEDLSAGLARKDFVLFSMYREGQITKEQYEEAKAYDLTKDFLPQQ
                     IAEQTDREFLYYTVMNEATRIIAQQLAEKDNADMSDSSVSDAYYQKAQQTIQNKGYTI
                     HSTIDKDVYAALQNGVENYGYMLDDGAGSQVETGNVLMDNRTGRIYGFVGGRNYSQNQ
                     NNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSESRVSDYATTWQEGENAGEKIVNATN
                     EGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGSKQYAYEHYLAKMNYPANDNWGVES
                     APLGTVDVTTLQQTNGFQALANGGVYEEGYIIDSITDNAGNVIYKHESNSVRIYSEAT
                     ASIMNDMMRSVINAKITTPFKDAISSLNGNLGKADWVGKTGSTNEYRDSWLIVSTPSI
                     TISSWAGHDDNTGMDSKARIRSANYLANLINQAYQAKPDIFGTDEKFELSSDVMKKKV
                     SGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGPKKSAFKFGVGGTDENYTDYWNTAS
                     AYARANQATKESDEKDD"
BASE COUNT         8852 a         4835 c         5907 g         7829 t
ORIGIN      
        1 aaaacttgca gcagctactg acagctgccg caagtttttt tgagtttcct cctcttttag
       61 atgaaatatg aataaattat gaagttctta ctttaatacg cttaagatat ttcaattatc
      121 tataactaat gataaaatga aaagtaaagt ctacatagtc tggataatac acagaaggaa
      181 aaggagatta cacaacgcat ggccaatttg attcgttcac ttatcgaaaa cgataagaaa
      241 gaactaaaac gtttagatag cattgcaaaa aaagttgaag catacgctga ccaaatggca
      301 gcattaactg atgaacaatt acaggcaaaa actgctgaat tcaagggcag ataccaaaaa
      361 ggagaaacat tagatcaatt acttccggaa gcttttgcag tcgtccgaga agcagcaaaa
      421 cgtgtattgg ggttatatcc acaccacgta caattgatgg gtggaatcgt gcttcacgat
      481 ggaaatatcc ctgaaatgcg tactggtgaa ggtaaaacct tgaccgcaac gatgccagta
      541 tatctaaatg ctctgactgg cgaaggggta cacgtagtta ccgtcaatga atatttagca
      601 acccgtgact cgacggaaat gggcgaactt tataatttct taggattaac agtggggttg
      661 aatatcaact caaaatcttc ggatgaaaaa cgcgaggctt ataattgtga tatcacttat
      721 agtacgaata atgaattagg atttgactac ttgagagaca atatggtcgt ttaccgccat
      781 caaatggtac aacgtccgct gaattatgcc attgtcgatg aagttgactc aatcttgatt
      841 gatgaagcga gaacgccgct gatcatttca gggcaagcag aaaaatccac agctttatat
      901 acaagagcag acaacttcgt gaaacgtttg aaagaagaag aagattataa aatcgatgta
      961 caatcaaaaa caatcagttt aaccgaagca gggatcgaaa aagcagaaga aacattcggt
     1021 ttggataact tatacgacat tgaaaatact gcattgaccc atcacttgga tcaagcattg
     1081 agagccaact atatcatgct tttagatatc gattatgttg tccaagacgg aaaagtactg
     1141 attgttgacc agttcactgg tcggatcatg gatggacgcc gttattcaga tggacttcac
     1201 caagcaatcg aagctaaaga aggcgtagag atcgaagacg aaacgaaaac catggcgacg
     1261 atcactttcc agaactattt ccgtatgtac aagaaacttg cgggaatgac tggtacagct
     1321 aaaacagaag aagaagaatt cagagaaatt tataacatcc aagtatacca gattccgaca
     1381 aatcgtccgg ttatccgtga tgatcgtcca gatttgcttt acccaacatt acaaagcaag
     1441 ttccacgctg tcgtgcaaga tatcaaagat cgctaccgca aaggtcaacc tgtgttagtc
     1501 ggaactgttg cggttgaaac atctgagtta ttatcagata tgctgaatca agaacgtatt
     1561 cctcatgaag tgctgaatgc gaaaaatcac tttaaagaag cagagatcat catgaatgct
     1621 ggtcaaaaag gtgctgtgac gattgctacc aacatggctg gtcggggaac agatatcaag
     1681 ttaggacttg gggtaagaga acttggtggt ctagcagtta tcgggacaga acgacatgaa
     1741 tctcgtcgta tcgataacca gcttcgagga cgtgcaggac gtcaaggaga ccctggtatg
     1801 tcacagttct acttgtcatt ggaagatgat ttgatgaaac gattcggttc agaacgaatc
     1861 aaatctttct tagatcggat gaagatcggt gaggaagatg ctgttatcca aagcaagatg
     1921 ctgacgaaac aagtagaatc tgcacaaaaa cgtgtagaag gaaataacta tgacacacgt
     1981 aaaaacgtct tacaatacga tgatgtgatg cgtgaacaac gtgaagtaat ctataaacag
     2041 cgacaagaag taatcatgga agaaaaaagc ttatccaagc cactaatgaa catggtcaaa
     2101 cggacaatca gtcgtgtagt agatgcgcat acacaattag agaaagaaaa ttggaatttg
     2161 gaaagtatca tcgattttgc cggcagtact cttgtccatg aagatagtat tactcttgca
     2221 gatatcgaag gaaaaacagc tcaggagatc aaagagtatc taaacgaacg agcacaagaa
     2281 gtatttaata ccaaagcggc tcaactaaat ggtccagagc aactgttgga atttgaaaaa
     2341 gtcgtgatct tgcgtgttgt ggactctaaa tggacagacc atattgatgc aatggatcaa
     2401 ttacgtcagt caatcggctt acgtgcttat ggacagaaca atccattagt cgaatatcaa
     2461 acagaaggct ataagatgtt tgaagatatg gtaggcgcca tcgagtatga agtcactcga
     2521 atctttatga aagctgagat ccgtcaaaac gtacagcgtg aacaggtttc tcaaggaaca
     2581 gcttctcact cagaagacgg agatgccaaa gaaaatagta atactagcgc gaaaaaacag
     2641 ccaatccatg tgaacaaggt cggaagaaat gatccttgtc cttgtggaag cgggaaaaaa
     2701 tataagaact gtcatggaaa aaatgcaaac taatttctga cagcaggatt ggtgagacaa
     2761 attctttttt taagaattcg tctcaccttc tttctgttat ttgctgattc tgttagacta
     2821 gatataatga aaaaagtata gtgaaagaag gaaaaaacat ggaaatatcc gaaattcgaa
     2881 atctcctgac agaaatgcag gaaaaggtca caagcttcag gaggtctctt tgacttagac
     2941 cagctggaag aagacattgc ggaagcagaa gcaaaaatgg ctgaacctgg tttttgggat
     3001 gatagcgaag ctgctcaaaa agtgatcagt gaaaacaatg caaataaaga aacttatgat
     3061 caattccatt ctcttgctga agaactggaa gaaatcgaat tgatggtgga gatgttgcag
     3121 gaagaacctg atgcagccat gcaagaagaa gcagaaacac gtatcaatgc gctagatgaa
     3181 aaaatgaaga catatgaatt atctatgttg ttagatggtc cttatgatcg aaacaacgcc
     3241 atcgttgaat tgcatccagg ggctggcgga acggagtccc aagattgggg cagtatgctt
     3301 ttacgtatgt atacacgctg ggcagagcag catgggtatc aagtagagac actagattat
     3361 caagcaggtg atgaagcagg aatcaagagt gtgaccctac tgatcaaagg acacaacgca
     3421 tttggttatc tgaaatcaga aaaaggtgtt catcgccttg tacgtatctc gccttttgac
     3481 tcagcgaaac gacgtcatac ttctttttgt tcggtagatg tgatgccaga gcttgatgaa
     3541 acgatcgata tagaaatcaa tccagatgat ttgaaaatcg atacgtaccg agcaagtggt
     3601 gcaggtggtc agcacatcaa taaaacagaa tctgctgtac ggatcactca cttgcctaca
     3661 ggaacagtag tagctagtca ggcacaacgt tcacaattga aaaaccgtga gcaagcgatg
     3721 gggatgttaa aagctaagct ttatcaatta gaagtggaaa agaaagaaca agaagcagct
     3781 gcacttcgag gagaacaaaa agaaatcggc tggggttcac agatccgttc ttatgttttc
     3841 catccatatt ctatggtaaa agaccatcgg acaaacgtag agacaggaaa tgtccaagca
     3901 gttatggacg gtgaccttga tttatttatt gacggctatt taaaaatgca tctacaataa
     3961 gaacccattg ttaaattgaa ataaaattgt aagattttgc aagcatgttg aaacattgcc
     4021 atgctataat agctaaagac tgaaaaagat atggagagaa cgccatgatt gaaatgaagg
     4081 atgtaatgaa gaagtactca aatggtacga ccgctatccg caatctctca attgtcatcg
     4141 aacaaggaga gtttgtctat gtggttggtc cgtctggagc cggtaaatct acattcatca
     4201 aattgatgta tcgagaagaa aaagcttcaa aaggaacttt gaacgtcgca ggtcatgatc
     4261 taatagcaat caaaaaccgc gaagttcctt atctacgcag agaaatcggc gtggtgttcc
     4321 aagactataa attattacca agaaaaacag tatatgaaaa tgttgcgtac gctatgcaag
     4381 ttatcggcag aaaacctcgg gatatcaaaa aacgagtaat ggaagtgctg gaccttgttg
     4441 gtttgaaaca caaagttcga gtctttccta gcgaattatc cggtggggaa cagcaacgtg
     4501 tctctattgc tcgggcaata gtgaatacgc ctaaagtact tatcgcggat gaaccgacag
     4561 gaaatctgga tccagaaaac tcatgggaaa tcatgaaatt attagatcgg atcaatgccc
     4621 aaggaactac tgtcgtgatg gctacacata acagtacgat cgtaaatacg attcggcacc
     4681 gagtaattgc tatcgaaaac ggccgcatta ttcgagatca agcggaaggg gaatatggat
     4741 acgatgatta gaaccttttt ccgccattta ttagaaagta tcaagagttt gaaacgtaac
     4801 ggctggatga cagttgcttc agtcagtgcg gtaacgatca cattgacttt agtcggtgta
     4861 ttcatggcag ttatcatgaa cgcaactaaa ttagctcaag atatagaagg aaacgttgat
     4921 gtctctgtat tcgtagatat cggtacaaaa gacgaagaga tgaaaaagct agaaaaagaa
     4981 ttgaatgact tagatcatgt taaaaaagtt gaattttcaa gcaaagaaca ggaattgaaa
     5041 aaaatccaaa acgaaatggg tgattcctgg aaattgtttg aaggtgacaa taacccgtta
     5101 tatgatgtgt atgtagtaag tgctactgat ccttcttata caaaacaagt agcagaagaa
     5161 gcagcagatt tgaagaatgt ttctcgttct gattacggtg gagcttcctc agatcggatt
     5221 ttccaaatcg ctggtgcagt tcggacatgg ggattagttg cagcggcatt gctgctgttc
     5281 gtagcgatgt ttctcatttc gaacacgatt cggatcacga ttttgtctag acagcgtgaa
     5341 attcaaatca tgcgtttagt cggtgcaaaa aatggatata ttcgctggcc gtttttcctt
     5401 gaaggtggct ggatcggtct gatcggtgcg attcttccga ttttgatcat cacatttggt
     5461 tatgcatggt ttttccaatt gatcaatcca tcattattac gttcccatta ttcattgatt
     5521 catccacaaa atatcgtttg gaaaatcaat cttttaatgg tcggcattgg tgtgatcatt
     5581 ggttcacttg gctcgatcat ttctatgaga cgtttcttga aattctaata ccacaatata
     5641 aataaaatga gaaagttgga cagtttcgtt ccagctttct ttttttattg gctaggagaa
     5701 tagaaggaag cggctttttt ccctttactt taaaaaacaa cttgggtatt cttaagttgg
     5761 tagaagaaga ggtgaagccg tgaaaattat cgttgacaat ttttttacga tccttttgat
     5821 tatgaatatt ttgctttcct ttatcatcgt ttttcgagaa agaaaggaaa ctgctcaaac
     5881 atgggcatgg gtgctcgttt tgatgttcat tccagttgta ggatttattt tatatatttt
     5941 tttaggaaga gggatttcca aagagaaaat ctttgatttg aagatccaag ggaaaatcgg
     6001 taagaacctg gaaattgaag aagacaaaca ggcattgatg cgagggcttt atcctcatcc
     6061 gcctactgga caagtagatg tgaaacaatt gatctatatg ctcacggtat ttgaaagtac
     6121 gctctatacg actaaaaatg aaatcacttt atttacagat ggtcgtgaaa aattcgatgc
     6181 gctgattgaa gatatcaagc aggcagaaga ccatatccac ttccagtatt atatttatcg
     6241 cagtgatgca ttaggtgaag aagttcgaga tgcattgaca gaggcggcaa gacgaggggt
     6301 aaaagtcaga gtccttcttg atgcatgggg ttccacacaa gtttcttcca gcttttttca
     6361 gaatttgaaa aaagcaggcg gagaagtcgc atttttcttt cctctatttg ttccgtatat
     6421 taatcctcgg atcaattatc gcaatcatcg taaaatcgta gttattgatg ggaaaatcgg
     6481 atatacaggt gggttcaatg ttggaaatga atatctagga ttggtaaaga aatttggtta
     6541 ttggcgagac aatcatttgc gtatttacgg agaagcggtg tataccttgc aaaaccgttt
     6601 tttgatggac tggaattctc agcatacaaa agaagtaggc tattctccca aattttttcc
     6661 gtccattcac tctactggtg atatagctgt tcaaatcgtg acaagcggac cagataccga
     6721 acatgaacaa atcaaaatga cttatttgaa aatgatttcg ttagccaaac gggagatatt
     6781 gatccaaacg ccttattaca taccggatgg atcgattcat gaagcactga agttggctct
     6841 tctttcaggc gtaaaagttc atatccaaat acctaacaaa ccagatcatc ttctcgttta
     6901 ctgggcaacg tattctttcg ctgcagagct aatcgagtat ggagcaagga tcgaaacata
     6961 tgagaatgga tttattcatg caaagacgat gattatcgat ggtggcattg tttcagttgg
     7021 ctcagcaaat atcgatgttc gttcatttcg cttggacttt gaggtcaata cactggttta
     7081 tgatgaacga atggcttcac gtgtaagaaa agcttttttt gaagactcga aaatttctac
     7141 gcatctcaca aaagaaatgt atgaaaaccg aggaataatc atcaagatga aagaaggatt
     7201 agcaagattg atttcgccgc tcctttaaaa cataaaaagg acatgagaac catcaacaga
     7261 tggagatctc gtgtcctttt ctaacggggc catttctgag agtacgtcag aaatggagaa
     7321 agcgtctcgc atgagcgaaa ttaactttgg ggtatctctt gttttttggg gaaagcaaga
     7381 aataagctaa tatatgctat taaaagagac acatttcaaa gccagtatag catagaaact
     7441 tttaatgaga tagttttttt tacaattatg agaatttatt ttgttattta atgattttga
     7501 ttcgttttca tcaagtaatg aaaatgataa aataaacata ctttaggatt aaaggagatt
     7561 tctaatgaaa aaacttaaat tattcttacc aataatagga ttagctattc ttttatcagg
     7621 ctgttcaaag tggattgatc gaggagaatc cattacggct gttggctctt ctgctttaca
     7681 gcctttagta gaaacagttg ctgaaactta ccagacagaa aatcctggaa aattcatcaa
     7741 tgtacaaggg ggcggaagcg gaactggttt gtctcaagta cagtcaggag cagtcgacat
     7801 cggaaactct gatttgtttg cggaagaaaa atcggggatc gatgcctcag cattagtcga
     7861 tcatcgagta gcagttgttg ggatcactcc tattgtgaac aaggatattg gcgtaaaaga
     7921 catcagtatg gaaaacctga aaaaactatt tacaggcgag atcaaaaact ggagtgaagt
     7981 cggtggaaaa gatcagccag tagttatatt gaaccgcgct tcaggaagcg gaacccgtag
     8041 cacgtttgaa aaatgggtat tggacggaga aacagctgtt caggcacaag aacaagattc
     8101 aagcggaatg gtgcgccaga tcgttgccga cacacccggt gcaatcagct atgttgcttt
     8161 ttcttatgtt tccgatgatg tagatacgtt gaatgttaac ggtgtagaac cgacagatga
     8221 aaatgtgaca acgaacaaat ggacgatttg ggcatatgag catatgtaca caagagatca
     8281 tccaaaaaaa ctgacaaaag aatttttaga ctatatctta tctgatgaag tacaagataa
     8341 catcgttgga gaactaggtt atatccctgt ttctcatatg aaagtcgaac gtgattggca
     8401 aggaaatatc gttaaagagt aatctttaca caaaatttac aaacttagct aaacttttac
     8461 atgttattaa aaccaaattg atgttcaatt tgtacactga gtgagtatgt taaacctgat
     8521 taagggggag tcatctgtgg aagatgtgaa aaaagtatta ttaacaaaat caaaacgttc
     8581 aaaaatggaa cagcgtggaa aattcatcag ttttttatgt atcgcgctga tcgtgttggt
     8641 tgtattatct attttttatt ttgtagctag taaaggattg gccacctttt ttactgataa
     8701 gatcaatgta tttgattttc tgtttggtac agattggaat ccaagccaaa tcggagcaaa
     8761 tgggaaacca atggtagggg cattgcctat gatcacaggt tcgtttattg tgaccttttt
     8821 atctgctatc gttgccacgc catttgccat tggtgcagca gtattcatga cggagatctc
     8881 tcctaaaaac ggaacgaaga tcttacagcc ggtcatcgag ctgcttgtag gtattccttc
     8941 tgtcgtttat ggttttatcg gtttatcagt tatcgtgcct tttgtcagaa gtatttttgg
     9001 cgggactggg tttggtatct tagctggtac atttgtgttg ttcgttatga tcttgccgac
     9061 tgtcacaacg atgactgttg atgcattaaa ggcagtgcca cggcactatc gggaagcttc
     9121 attagcgctt ggtgccacaa gatggcaaac aatctacaaa gtcgtgctac gagcagccgt
     9181 gcccgggatt ttaacagcag tcgtttttgg tatggctcgt gcgtttggtg aagccttagc
     9241 tatccaaatg gtcatcggta atgcagcatt gatgccaaca ggtcttacta ctcctgcttc
     9301 tacattgacg tccatcttaa caatgggaat cggaaataca atcatgggaa cagttgaaaa
     9361 taatgtgtta tggtcattag cgctgatctt gctattgatg tcattattct ttaatatcgt
     9421 gatccgtatg attgggaaga aaggagagct gaaataatga acgcaaaacg ctcagataaa
     9481 attgctacag gtgttttata tatagtttcg gggatcatcg tattgatttt agcagcttta
     9541 ctactttata tcttagttcg gggaataccg cacatttcat ggtccttctt gaccgaacct
     9601 tccaaagcct accaagaagg cggtggaatc gggatccagc tatttaactc gatttatttg
     9661 cttttgatca cgatgatcat cagttttcct atttctttag gtgcgggtat ctatctttca
     9721 gaatatgctg gaaaaaactg gctaacagat gtgattcgca cgtcgattga aatcttgagc
     9781 tcgttgcctt ctgtcgttgt cggtttattc ggtttcttgg ttttcgttat tcaattccag
     9841 tatggtttct cgattatttc tggtgcattg gctttgactg tattcaactt gcctcttttg
     9901 actagaacag ttgaggagtc tttacaagcg gtccattaca cacaacgaga agcaggattg
     9961 gccttaggtt tgtcacgctg ggaaacagtg ataaaagtgg tggttccaga agcgacacca
    10021 gggattttga ctggtgtgat cctaagttct ggaagaattt ttggtgaagc agctgcattg
    10081 atctatacgg ctggacaaag tgcacctgcg ttagatttta cgaactggaa tccattaagt
    10141 gtttcaagcc cgatcagcat tttccgtcaa gctgaaacat tagctgtcca tatttggaag
    10201 atcaatacag aaggcacgat gcctgatggt gtttctgttt cggctggcgc ttctgccgtg
    10261 ttgattattg ctgttttact gttcaacttc ggcgctcgtg cgattggcaa acgattatac
    10321 cgaaaaatga cttcagccta aggagaagaa gcaaaatgaa aaaatataac tggaatgaaa
    10381 caaatgtcat caccatgcca aaagagaaag aagtagcact atatacggat gatctacatg
    10441 tctggtatgg cgacaatgaa gcaatcaaag gagtggatct ggaatttgaa aaaaataaga
    10501 tcactgcact gattggtcct tccggctgcg gaaaatctac gtatcttcgt tcgttgaatc
    10561 gaatgaatga tgggatcgct aatacgaaaa tcactgggaa aatcatgtat caaggcgtag
    10621 atattaatgc gcctcaagtg gatgtttatg agatgcgcaa acggattggc atggtttttc
    10681 aacgtcctaa tccgttcagc aagtcgattt atgacaatat tacatttgct ttgaaacagc
    10741 acggcgaaaa aaacaaaaag aaattagatg aaatcgttga aacaagcttg aaacaagctg
    10801 ccttgtggga ccaagtaaaa gacagcttgg acaaaagtgc cttggcttta tctggcgggc
    10861 aacagcaacg tttatgtatt gctcgtgcga tcgcgatgaa acctgatatc ttgctgctag
    10921 acgagccggc aagtgctttg gaccctattt cgacaggaac ggttgaagaa acacttgtaa
    10981 acttgaaaga agactatacg atcatcatcg tgacgcataa catgcaacaa gcagctcgta
    11041 tcagcgacta tactgccttt ttctatttag gaaaagtaat ggaatatgac aaaactaaca
    11101 agatatttac cagaccaaaa attcaggcaa ccgaagatta tgtgtccggt cattttggtt
    11161 aagacggagg cctcaacatg acaaaagaga ttatttcatc taaagatctt catctgtatt
    11221 atggaaagaa agaagcacta aaagggatcg atctaagctt caatcaaggg gaaatcactg
    11281 cgatgatcgg tccttctggt tgcggaaaat ccacttatct gcgttctttg aaccggatga
    11341 atgacctgat tccgaatgta acgatcaccg gaagtgtttt atataaagac aaagacatct
    11401 atggtccaaa aacagatgtt gttgaactaa gaaaagaaat cggcatggta ttccagcagc
    11461 caaacccatt tcctttttct atctatgaaa atgtcatcta tggattgaaa ttaaaagggg
    11521 aaaaagataa aaaagtcctt gatcaagtag ttgaagaaag cctgaaggca gcgtctgttt
    11581 gggatgatgt gaaagacaaa ttgcataaaa gtgctttatc tttatccggt gggcagcagc
    11641 agcgtgtatg tatcgcacgt gttttggcag ttaatccgga aatcatctta ttggatgaac
    11701 caacaagtgc gcttgatcct gtttctactg ggaaaatcga aagtatgctg ttagaattaa
    11761 gagagcggta tacgatgatc atcgtgaccc ataatatgtc tcaagcttca cgtatttcag
    11821 ataagacggc attcttttta gatggtcact tgatcgaata caatgatacg aagaagatat
    11881 tcatgaatcc agaaaaacaa gagacagaag attatatttc aggtcgattt ggataaggag
    11941 gaaacggaat gctacgtaca caatttgaag aagaattgct gaatctccat aatcagttct
    12001 acgaaatggg gatgatggtg agcagcgccg tgcacaagtc agttcgtgca tacatcaaac
    12061 acgataaaat cttagcccaa gaagttatcg aaaacgatat aaatatcaat aacatggaaa
    12121 cacgcttgga gaagaaaagt tttgaaatga tcgcattgca acagccagtt acaactgatt
    12181 tgcgtatgat cattacggtc atgaaagcta gctcggattt agaacgtatg gccgatcatg
    12241 cggtgtctgt tgctaaatcc acgattcgcg taaaagggca gacgagaata ccggaaatcg
    12301 aaaaagagat ctccgatatg tccgattatg tgaaaaaaat ggtggacaac gtcctagttg
    12361 cttatgtcaa aacagacgaa aaagacgcac gtacgatcgc caatatggat caaagagtca
    12421 atgactattt caatcgaatc tataatgcaa caatcaaaaa tatgcaggaa aatcctgaga
    12481 cagtcatcag tggaacagac tacttgaatg ttgccagtta tctagaacgg atcggagact
    12541 atgtgacaaa catctgtgaa tggatcgtct acttggctac agggaaaatc actgagttaa
    12601 atacgaacca caatgagatt ttctaaactg aagctgtgat tatgccgttt gtttaggaaa
    12661 agaggttgtg aaatcagcgg ttgtgaaaaa gctgttctgc atcaagtaat aagaacagtc
    12721 aaacaaaaaa tagctcctca tatttttcgt ttggctggac ttattaccgc agatgcaagc
    12781 ttttgaacac cgtttattcg ggaaagggtt tgtgacaaaa catgtcacaa acccttttca
    12841 tcatttcata gaaaggataa agaatgcaaa aaaaaattag catcatcact atatttttta
    12901 tttgtgtgct tacttcatgg gttattcagc atcaagccgc tttatatgaa gtacctgtca
    12961 tcgagataga aaaagtacga ataaaagagg atcatcaatt gattacagga aaactgatga
    13021 acacggatca aaaaggagaa tcgatttatt tatcggttcc ttatcaaaaa aatcaaatag
    13081 aaacagcaaa tataaaagcg agacaaatgt atctagtcca aatgcatgga gatgaatggc
    13141 agctagtagc aaaaaaaaat gatggctggc ttttcttctt cacaagtatc ttcattggcg
    13201 ccatgttgct tattggtgga aaacagggtc tatttgcttt aggaagtctc atcatcaata
    13261 tcgttttact tttacttttg ttgctcatct ttactaaaac agaagggatt tctctattaa
    13321 atatatctat cctgttcgtc gttataggga tgattattag catattggct ttagatggat
    13381 ggaatagaag cagtataatc aaaatttcag ctgcagttac gagtgttttt ttagcatttt
    13441 ttatttgtct tttgacaatg aatcattgga aagaccaagg actgcgctat gaggaactga
    13501 attatctgac acgaccgtat cgttctgttt ttctttctag cgtactgatt ggaacagctg
    13561 gaggaacact cgataccgtt atcacagtca ttgcgacatt agaagaattg actaagcgac
    13621 aaccagaaat aacagcgaaa cagttatggc tttcaggaag gcaagtgggt cgagatgtga
    13681 ttggctcgat gaccaatatc cttttgttcg tctatatcag tgggtcagtt ccatcgcttc
    13741 ttttatattt aggaaatcaa tggtcgttca aagaaacgat agaacagcat ttatcattgg
    13801 aatttttacg cgtcattgct ggcagcttag gaatagtatt atcgatccct atctcggtcc
    13861 tcttttttct gactgtaagg aggctgaaac gatgaatgta cttattatac ttgttgggat
    13921 tttagcaatc agcgtgctac ttattggcgg tacgcaaggt atgtatattc ttttaggctt
    13981 attcatcaat ttagggatct ttttcctgtt gttattcggt tatcatcaga agtggccgat
    14041 actggcattg tcggtcatag gatttttatt gattgctgtg gttatcttat tttttatcaa
    14101 cggttataat ctaaaaatgc gtgcagcttt tgtgagtata ctgatctttt tattttgttt
    14161 cctactattg cttcctatca ctgatttttt agcaatccaa ggttttacat cggtagaact
    14221 agaagaatta tctggcttag ataagacttt agcgattgat tttagattac ttacgagatc
    14281 gttgcttctg attagtctta gtggtgctgt tttagatgcg agtgtagcaa tttctagtgg
    14341 gacattcgaa gtatatcagg caaatccgca attatctttt taccaattgc gccattctag
    14401 tttcgctatt gctaaaaaaa tattggcgtc aacagtaatg acacttcttt ttgcattcat
    14461 gggaagttct ttagcgttga tcctctggtt tattgattta gacattcctt ttcagcaagt
    14521 gatcaatgaa aaatcatttg ttctagaata tacgatggct attcttacca cattgtccgc
    14581 actacttgtt cttcctttaa cctgcgtgac tacagcatat ctctttacaa aaaagaaaga
    14641 acaactcaag atggagtaaa agttacaact taagtcctat gacattttta ctgaatcagt
    14701 gcataatatg gatgtaagga aaagcttgca aacaattttg cttaaggagg caatttccat
    14761 gaaagaaaga gaacgtattt tagatttagt gaaaaaagga atcctttcga ctgaagaagg
    14821 cttagattta ttagaaagca tggcaactga aaaagacgaa aagcaaatca aaaaagaagc
    14881 agacaaggta actgctagtc ataaagaaaa agatcaggca agtcaattga tcgataaact
    14941 tgaaaacggc gaagaagaaa tttctgagcc agttgatcca aaggaaagag aaagacaaga
    15001 tcaagaaaac ttagaaaaga tcttagatga actagcaact gaagcgaata aaacttcggc
    15061 gcgtctagat gaagtaaatg ctcaattcgc tgacaataag gcagcacgca atgagaaaca
    15121 ggaagcattg atgcagctga atacaaaaga agaattaggc gagttgacag aagaagaatt
    15181 agcacagcgt cagacgttag aagcagaaat caaagaatta gaagctagtg gcgatacact
    15241 gttagaagaa caattgaaat tagaagcaga attaaaagat atccgtaaga atcaatggtc
    15301 tgagaaaaaa gaaacgttta ctgataagtt tgaattgcca gatgattgga aagatcaagc
    15361 aacagataca ctgaatcaag taggcgaaaa aatgtcagaa gctggatcac agttaggcaa
    15421 attcttgaag aaaacattcc aaactgtttc agaaacagtg aatgacaaca tggaatggaa
    15481 agatgtcagc ttacgtgtcc ctgggattgc gacaacgaaa tttgagcatg aattttacta
    15541 tgaagctcct gcagcaagta tccttgatat caaggcagca aacggaaacg tgacgttaaa
    15601 aacgtgggac agcaacgatg tgaaagttga agctaagatc aaactttacg gaaaaatggg
    15661 tgctgaacca tttgaagcat tctctgaacg gagccagatt gaggttaatg aagatcatat
    15721 ctccttccaa attccaaata aacgtgtgcg tgcagacctt gttttctact tacctaaacg
    15781 agtatacgac cacgcagcta ttaaacttct aaatggcaac atcatgattg aaacattgga
    15841 agctaaagat atttatacaa aatctacgaa tggtaacatc atcgtggacc aattaactgc
    15901 aacgatgctg gaagtagaag gtgtaaacgg caatatcgat attcgaaacg ggaacatctt
    15961 agattctatt atcgaaacag taaatggtac agtgacattt ggtgcgacag cagaaaacct
    16021 ttctgtgtct ttggtaaatg gtgatgtacg tcttacgatt aaagaagata atttgaaaaa
    16081 agtagaagca agttcagtga acggaaatgt caaagtcgct ttaccagatg gcatcggttt
    16141 ggaaggacat gctaaaacaa gtcttggaag tatcaacagc cgtctgtcca attacgaagt
    16201 agttcgtgag aaaaaagaac ggacaaacca aatgttgcaa ttccgtcgtg tttcagatgg
    16261 tgaaatcgcg caagtgcaac tttcaacgac aacaggaagt atctatttaa aagatacaga
    16321 taaataaaga ggttgtgaaa gagatgttca gccttgagca ataagaagcg attttgtaaa
    16381 atagcttccc ctattttaca aaattgtgcc ttattgccga tagtctatct cttgaacacc
    16441 gtttaccaag agattatgaa cctgccaatt atcgccgagt cacaaggaat aatttcagaa
    16501 aaagctcgtc acgttttatg aatatggtaa tttgtgaccg agaattttgc agaaagacac
    16561 cgattaagaa gaaggttgtc acaacgattt tcttcattta aaatagtaga acagaggtga
    16621 gacatgatga aaaaaaagtt gacaaaatct aataaaaatg ttgttttgac agggacttta
    16681 gcaggaatag gagaatacat cgggattgat ccgactgtta tccgtgtgct ttatgtattc
    16741 ctgagcttag ttgcttttgg aagtccaatt attttataca ttttattagc tttgattatc
    16801 ccatctgcgc gttctgcacg aacaaactat ggacataaca atgcatatta tgaaaaaaac
    16861 aattatcgtg aacaaactcg tgaacaaaca aaaaaacgca aagaagcgga aaaaatcgat
    16921 gatgacgaat ggagtgactt ttaatgtcgt actttcaacg tattttagtt aatacgttaa
    16981 cattcatatc attggctgtt ctttttcctg cgatggtcca tgtgagcagt atttggacgg
    17041 ccgttcttgc cagcttcgta ttgtctgtgt tgaatatgtt tgtcaaaccg attttgacga
    17101 tactgtccct gccctttaca ttagtcacat tgggattgtt tacgtttgtt atcaatgcag
    17161 cgattcttaa aatgacctcc ttttttgtgg gagcagaaca ttttagtttt tcttcattcg
    17221 gtgcagcgtt gttagtagct atcttgatgt cgcttgtcaa tgtaattgtc agcgaacata
    17281 acagtagaaa ataaaagaaa gctgtgccga actatcggcg cagttttttt atttagaatc
    17341 aggaatgcgt tttctattac attggtaatt aaataaaaac gtttttttta attggtagta
    17401 cttttggcta gacaatgata aaatgataga gaacactcta atagaaatga ggtagcatga
    17461 atgtcagaag ttgtaaaagt tagccaatta gtgaaaaaat tatctttgga aatcgtgaca
    17521 ggcgacgaga aaagcttaga ccgtgtcatc gtaactgggg acatctcacg cccaggacta
    17581 gagttgactg gttattttaa ttattattcc cataatcgtt tacagctatt tggcagtaaa
    17641 gaaatcagtt ttgcggaacg gatgatccca gaagagcgtt tgatcatcat gagaagatta
    17701 tgtagtgaag atatgcccgc atttattatt tcaagaggat taccagctcc agaagaaatg
    17761 atccaggcag caaatgaaaa tgggatagct gtactgcgtt ctcccatctc aacttctcgt
    17821 ttattaggag aattatccag ttatttagat ggtcgccttg cccctcgaac aagtgtccac
    17881 ggtgttttag tggatgttta cggattaggt gtactgattc aaggtgacag tgggatagga
    17941 aaaagtgaaa cagcgttgga gttgatcaaa cgcggccatc gattgatcgc agacgatcga
    18001 gtagatgttt atcagcaaga tgaattaacg gtgatcggag aaccgcctaa aatcctggaa
    18061 catctgattg aaatccgagg aatcgggatc attgatgtaa tgaatctttt tggtgcgagt
    18121 gcggtacgtg gctctatgca agtccagttg gctgtttatc tggaagcatg ggcaaaagat
    18181 aagaaatacg accgtctggg ctctgaagac acgaccgttg aaatcgcgga agtgggcatt
    18241 ccacaaataa aaatccctgt caaaacagga cgaaacgttg caatcatcat tgaagtagcc
    18301 gcgatgaatt tccgcgcacg tacaatgggc tttgacgcaa cgaaaacatt tgaagaaaga
    18361 ttaagtcgct tgattgaaga aaactcaggc aacgactagc aaggagtgac aatatgttag
    18421 cacaaatcaa tcgagttgct tttgagcttg tagggattcc tatctactgg tatgcattga
    18481 tcatcgtatc aggtatcatt atcgctatgt ggcttagcag cagagaggct gttcgggtag
    18541 gattaaaagc agatgatgtg acagatttta tgttgtgggg attgcctatc tccattattg
    18601 gcgctcgctt atattatgtt ttatttgatc ttccccagta tatagctgat ccaatccaaa
    18661 tattcaatat ccgttcagga ggcttagcta tttatggcgg actgattgca ggcgcgatca
    18721 cgctgtattt ttttactaaa tatcatttta tttctttgtg gactttttta gatatcgctg
    18781 caccaagtgt attattagca caggcaatcg gtcgttgggg aaatttcatg aaccacgaag
    18841 catatggacc agacacgacg cgaagcttct tagaaagtct gcatttaccg caattcatca
    18901 ttgataatat gtatatcgaa gggatctatc gtcaaccaac atttctttat gaatcacttt
    18961 ggagtttgat tggatttatt ttgcttttgt ttttacgaaa gaaaccacac cttttgaaaa
    19021 aaggcgaagt ccttttatgt tatctaatgt ggtatagttt cgggcgtttc tttattgaag
    19081 gattacgtac agacagtctt tactttttag gtgtgatccg tatttctcaa ttattatctg
    19141 ctgtgttatt tgtcggtaca ttgattctat ttatttggcg cagaaaaaaa ggaaatttac
    19201 cggattatga ccgctcatta ggaaaaaatc aacaaattat ctgattttta gagcgttaaa
    19261 aatgaaaaaa atttcaggag tggtaaaatg aattatagag tagcggtttt aggaccaggc
    19321 tcttggggta ctgctttggc acaagtcctc gccgaaaacg gacatgacgt tcggatttgg
    19381 ggacatcgtg cttcacaagt agaagaaatc aacacccaac acacgaatca acgttattta
    19441 ccagatcatt atctacccga ttccatccaa gcattcacca atatggaaga tgcagtaaaa
    19501 gacgcagatg cggtgctatt cgtcattcca acgaaagcaa tacgttctgt tgcaaaagaa
    19561 ctagtgccta aattgaatac aaagccagtg atcatccatg caagtaaagg attagaacag
    19621 ggaacacata aaaggatctc tgacatcttg ttagaggaaa tcccagcaga aaaaagaaaa
    19681 gctgttgttg ttctttctgg accaagccat gcagaagaag tagcaaaaca tgacatcact
    19741 acgatcacag ctgcaagtac agatgcaaaa gcagcaaaat atgtacaaga gctgttcatg
    19801 aataattatt ttcggatcta taccaatcct gatgtgatcg gtgtagagat gggggcagct
    19861 ttgaaaaata ttattgctat tggtgctggc gctattcacg gcttagggtt tggcgataat
    19921 gcaaaggcag caatcatgac gcgcggatta gctgagatca gccgtttagg tgtagcaatg
    19981 ggtgccaacc cgctgacctt tatcggatta agcggaatag gcgatctagt cgttacttgc
    20041 acaagtgttc attctaggaa ctggcgcgca ggcaacttgt taggacaagg gcagccccta
    20101 gaagaggtgc tagccaatat gggaatggtc gttgaaggtg tgaatactac ccaagcagcg
    20161 atggaactag cccaaatgat ggaaatcgaa atgcccatca ctcaaacaat ctatgaagtg
    20221 ctgtatgaac aaaaagaaat caaaacagcc gcaaaagaaa tcatgttacg tgatggaaaa
    20281 aaagaaaatg aattctcatt aacaattttt tgaaggagcg aatgtatatg aaagttaaaa
    20341 aagcagttat tccagcagca ggattaggaa caagattttt accagcaaca aaagctatgg
    20401 caaaggaaat gttgccaatt gttgataaac caacaatcca gttcatcgtt gaagaagcgt
    20461 tgaaatcagg tatcgaagac atcttgatcg ttacaggaaa agctaaacga ccaatcgaag
    20521 atcactttga ttcaaatatt gaattagaaa caaacctaag tgaaaaagga aagaccgaat
    20581 tattgaaatt agtagaagaa acgacagatg tcaacttgca tttcatccgt caatctcatc
    20641 caaagggttt aggacatgcc gttcttcaag caagagcctt tattggaaat gaaccatttg
    20701 tcgttatgct aggcgatgat ataatggaag acgaagtacc attgacacag caattaatcg
    20761 atgattacga acgtactcat gcttctacga tcgccgttat gcaagttcct catgatgaaa
    20821 cttcaaaata cggaattatc aatccaggag aagtattgga agatgggtta tacaatgtca
    20881 aaaactttgt tgaaaaacca gatccaagca aagcaccaag tgatttagct attattggac
    20941 gttacttatt gactccagaa atttttgatg tattgtccga gcaacaacca ggagccggaa
    21001 atgaaatcca actaactgac gcaatcgata cattgaataa aacacaacgc gtatttgccc
    21061 gtaaattcac aggtaaacgt tacgatgtcg gtgacaaatt tggttttatg aaaacaagta
    21121 ttgaatatgg tctaacacat ccagaagtag gtgcaccttt acgtgagtac atcctgtctt
    21181 taggtaaaac attagaagca gaaggaagca agatcgatca taatccaaaa ggaataacag
    21241 aaatcaaaga agaaaagaaa aaataaccct tttttctaac gagcttcagc tgctaattga
    21301 ataaaaagta gttgaagctc gtttttattt gtttaaaaaa cacgaaaatg ctatccttat
    21361 agaaaagaga gacaagaagg gagtttgatc atgtcaggag gagaaattgc aggattgatc
    21421 gcagcaatcg cctttgcgat acttgtcgtt tttatcgttc gtgtgttgtt gcaagtttct
    21481 aaaactttag aaaaagtaga tcagactgta gcagaagcaa atacgacgat cgaaatcgtc
    21541 acaaaagacg tagatctttt gtctagacaa gtagaaggct tactggtcaa aagtaatgaa
    21601 ctgctcttgg atatcaatgg aaaagtcgca acgatcgatc cattgttcac agcagttgct
    21661 gatttaagtg aaagtgtatc agaacttaat cattctagta aaaacatcgc gacgaaagtc
    21721 ggcggaatcg gtaaaacgac agctaaagcg acggttgccg gaaaagtcgg tgggacagcg
    21781 atgaaatttt ttaaaaacaa aaaacatact gaaacgacag gaggaaaata aaaatggcta
    21841 aaaaaggtgg atttttatta ggtgcagtag tcggaggcac agccgcagca atcgccgcgt
    21901 tattgttagc accaaaatct ggtaaagaac ttcgggatga attagctgcc caagcggatg
    21961 atttgatgga tatggcttca gattacacag atatggcgaa agaaaaatcg actgagttaa
    22021 caggtattgc aaaagaaaaa gcgcaagatc tttctcaaca agcaggtgat ctagcaggta
    22081 gtgtcaaaca aaaagtaagt gaatcagcag atgaagcagg aagtgaatct gacgatatct
    22141 tgttaaatct taaagaacaa tcagctgact tatctaatcg tttcaaaaaa acggcagaag
    22201 atatgaaaaa acctgcagat gaattaggaa gtattgctaa agatacttca gaagatatca
    22261 tgattgatgt aaaagacgct gctggtaaag caggagatac tgtcaatgaa ggaatgtcag
    22321 aagcaaaacc agttgcagat gactcaaaag aaatgatgaa aatggcaaaa gaagatgcga
    22381 aagaagctaa aaaagaaata aaagatacaa tgacagattc atctacaaat cactaaaatc
    22441 acaaatttat agaataaaaa ggtgggagcg gactagctct cacctttttt actcttattt
    22501 ttctaaaaca agtaattctt ttggtgtagt cgtgaatggc acacagccat cgcttgtcac
    22561 atatacacag tcttcgatac gtacccctac tttttcagga atatagattc ctggttcaag
    22621 agagaagcac atcccttctt cgattactaa gtcatttcct tctacaaggg aagggaattc
    22681 gtggacggtt gttccgatcc cgtgacctaa acggtgattg aaatattcgc cgtagccata
    22741 gctgttgatc acgtttcgtg cgatttgatc aagttcacct gctgtcacac ctggacggac
    22801 ggcttcttgt gcggcaagct gtgcttcaag agtgatgttg tagatttttt cttggaagtc
    22861 ggttggtttt tgataagcaa ttgtacgagt agcatcactg caatagccat tccaaacaac
    22921 gccaagatcg aataacacaa gttcattcgg tgaaatcttc gtatttcctg gtgtaccatg
    22981 agggcttgca gcatttgctc ctgcaaggac taatgtgtcg aaagacattt gtgaaacacc
    23041 ttgttttttc aattggtatt caatttctgc aatgatcgct tgttcagtca cgccttcttt
    23101 cactgcttta aagccaatct caaatgcaac atcggcccat ttaccagctt cgatcagttt
    23161 gtctttttcc tctagagttt tgatcagttg tagacgttgg atgatggaag ttagatcagt
    23221 gtaagaatcc gttcccgtca agatatcaga gatagcatcg aagcgctcga cagtcaaggc
    23281 gttcttttca atccctacat tgccaggctg accatagcga gcacgaagtt cttttccgat
    23341 gatctcccaa gggttttcgc tgtctaaata gccacggaca tcatacgccc aactactgtt
    23401 ttgagcatct tcaacttcca aagcaggagt gaaaaggaaa ggatcttgtt caagagggat
    23461 aaaaagagct agtacacgct catgaggatc actttcgtat ccggaaaagt aagcgatgtg
    23521 gccagggtcg ctgatataag ccaattcgac ttggtgttgt gccatccatt gacgtagttc
    23581 attgattttt acgttattca tttggcaacc tactttctaa attaattttg tattcaataa
    23641 ttgtaccact gatttattaa aagaccaacg ttctaaaaac tgaaaaaagt aaaaattttc
    23701 atgaaaacag agataaaacg gttgcaattc ttgagattat ttgatattct attttagaaa
    23761 tgtaagacgt attttcagaa aacatataaa atatgaatag gagaacacaa atggaaaagc
    23821 aaacaatcac tatctatgat gtagcgagag aggcaaatgt ttcgatggct actgtttcac
    23881 gtgtcgtaaa tggcaatcca aacgttaaac cagcaacaag aaaaaaagtc ttggaagtta
    23941 tcgatcgctt ggattatcgt ccaaacgcag ttgcgcgtgg cttggcaagt aagaaaacaa
    24001 caacagttgg tgtaatcatc cctgatgtca gcaatatgtt ctttgcttct ttagcacgtg
    24061 gtatcgatga tgtggcaaca atgtacaaat acaatattat cttagcaaat tcagatggca
    24121 acgaccaaaa agaagtcaat gtattgaata acttactggc taagcaagtg gacggcgtga
    24181 tcttcatggg tcaccatatc acagacgaaa tccgtggaga attttcacgt tcaaaaactc
    24241 ctgttgtatt agctggctct attgatccag atgaacaagt aggtagcgta aacattgatt
    24301 atgtatccgc aacaaaagat gcagtaaaca aattggcaaa aagcggcaat aaaaaaatcg
    24361 cttttgtcag tggtgcgttg atcgatccca tcaatggaca aaatcgtctg aaaggctata
    24421 aagaagcatt aagcgaaaat aacctaccat atagcgaagg attgatcttt gaagcacaat
    24481 acaacttcaa agatggtcta tctctagtcg atcggatcca taatagcggt gcaacagctg
    24541 tctatgtttc agatgatgaa ttagccatcg gtattttgga tggtttatta gaccgtggcg
    24601 tgaaagtacc ggaagaattc gaaatcatca caagtaacaa ttcactactg acagaagtag
    24661 ctcgtcctcg tttaacaagt atcacacaac cattatacga tttaggggcg gtatctatgc
    24721 gcctattgac gaaaatgatg aataaagaag aagtcgaaga aaaaacgatt atcttgcctt
    24781 atggaatcga agaaaaagga tctacaaaat aataaaagga caaccattca gaggttgtgt
    24841 gcctgccgtt taactctgcg ttacaaggag caatttcaaa aaacagcatc tcatgtttga
    24901 tgaatattgt gactcgtgat tcggagttgg caggagaaca ccgtttattt gggaaaaggg
    24961 gttatgacaa aattttgtta taatcctttt tttgtgtctt aaaaaagaga gaaagtggtt
    25021 gaaattacat ttatcgtata taaaacaatc cgtgtataga tgctgcattt tgttcaccac
    25081 catcaaaaaa ataagggact gtgacaatac tattgtcaca atcccttatt actgtgtatt
    25141 ctattaaatc aatcatcttt ctcatctgat tctttagttg cttgattcgc tcttgcataa
    25201 gctgaagcgg tgttccagta atctgtatag ttttcatcgg taccgccaac gccaaattta
    25261 aaggcgcttt tcttcggtcc atttttcgcc catagcgatt caactgtttt tcctggtgtt
    25321 tggatggatt ttttattgac agtaactttt ccaggtgttt gaccagtaaa tccagatact
    25381 tttttcttca tcacatcaga tgataattcg aatttctcat cagtgccaaa aatgtcaggt
    25441 tttgcttggt aagcttgatt gatcagattg gcaagataat tagccgaacg tatccttgct
    25501 ttgctgtcca ttccagtatt gtcatcgtgc cctgcccagc tactgatggt aatagacgga
    25561 gtagagacaa ttagccatga atctcggtat tcattggtag aaccagtttt tccgacccaa
    25621 tctgcttttc ctagatttcc gtttaagctt gagatggcat ctttgaatgg tgtagtgatt
    25681 tttgcattga tcacactacg catcatgtca ttcatgatcg atgccgttgc ttccgaatag
    25741 atccggacag agttgctttc gtgtttataa attacatttc ctgcgttatc tgtgatggaa
    25801 tcgatgatat agccttcttc atagacgccg ccgtttgcta aagcctgaaa tccgttggtc
    25861 tgttgaagcg tcgtgacatc tactgtacct aaaggagcag attcaacgcc ccaattgtca
    25921 tttgccgggt agttcatctt tgccaagtag tgctcgtaag catattgttt cgatccaccg
    25981 gaattcaaca catcttgata taagtgataa gccgggatat tgttggacca ttctagtgac
    26041 tcacggacgg tttggaaggt gttcgatcct tcgttagtag cattgacgat tttctcgcca
    26101 gcattttctc cttcttgcca agttgttgca taatctgata cacgggattc tgatccgata
    26161 agccccatgt cgattgcagg tccataaaca agaactggtt tgattgatga gccagcttga
    26221 cgttctgtat cgaaggcatg gttattttga ttctgactgt aattccgtcc gccaacgaat
    26281 ccgtagatgc ggccagttct gttatccatc aatacatttc cggtttctac ttgactgcca
    26341 gcaccatcat ctaacatgta tccataattt tcaacgccat tttgcaatgc agcatagaca
    26401 tctttatcga ttgtcgaatg gattgtatat cctttatttt ggatcgtttg ctgtgccttt
    26461 tgataataag cgtcactgac cgatgaatca gacatatcag cgttgtcttt ttcagccaat
    26521 tgttgtgcaa tgattcttgt tgcttcattc atcacagtat aatacaagaa ttctctgtca
    26581 gtctgttctg cgatttgttg tggtagaaag tcttttgtaa ggtcataggc ttttgcttct
    26641 tcgtattgtt ctttcgtaat ttggccttca cgatacatac tgaataaaac aaaatctttt
    26701 ctggcaaggc cggcagaaag atcttctttt agcgcgcctg tgttcgtgta tggactatac
    26761 gtgatcgggc tttggggtaa tccagcgata taagcagctt gcggcagcgt cacatctttt
    26821 gcatctacac caaagattcc ttttgcagct tcctggactc cagcaatgtt ttgtccctta
    26881 ttattacgac caaatggtga gacattcaaa tacgtagcaa tgatctcatc ttttgaaaag
    26941 tatttttcta cttgtgcaga aagaatgatc tcgtttgctt tacgtttgaa tgttgtttca
    27001 tctgtaagga tctgctgttt gactaattgc tgtgtcaaag ttgatccacc agaagagcca
    27061 ataccagtag cttcagatac taatgctcgt aaaacagctt taggaacgta accttggtgt
    27121 ttatcgaagt attcatcctc tgtgctgata attgcagttt tcaataaagg tgaaatctta
    27181 tcagatgaaa tagtcgtgcg catcagatct gtctggattt ttgatatctc tgtattatct
    27241 gcataaacaa tctttgacgt ttctgtaatg tttcctagtt ctgtctgcag tgctttttta
    27301 gtcggcaatt gtgtgtcttc gacgagatag gcaaaatagc cagcgcctat tccaatacct
    27361 agagctgcga acatcaaaag aagaacagta ataaaaacag tcaatgattg aaaaactcta
    27421 aaa
//
LOCUS       DARBDV010000030        26377 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000030 DARBDV010000000
VERSION     DARBDV010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.030"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(220..1512)
                     /locus_tag="SCM56_001209"
     CDS             complement(220..1512)
                     /locus_tag="SCM56_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG1110025.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(1509..2132)
                     /locus_tag="SCM56_001210"
     CDS             complement(1509..2132)
                     /locus_tag="SCM56_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110026.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(2135..2971)
                     /locus_tag="SCM56_001211"
     CDS             complement(2135..2971)
                     /locus_tag="SCM56_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1110027.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(2988..3875)
                     /locus_tag="SCM56_001212"
     CDS             complement(2988..3875)
                     /locus_tag="SCM56_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1110028.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(3883..5118)
                     /locus_tag="SCM56_001213"
                     /pseudo
     CDS             complement(3883..5118)
                     /locus_tag="SCM56_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(5801..6484)
                     /locus_tag="SCM56_001214"
     CDS             complement(5801..6484)
                     /locus_tag="SCM56_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEG1110029.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(6502..7119)
                     /locus_tag="SCM56_001215"
     CDS             complement(6502..7119)
                     /locus_tag="SCM56_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEG1110030.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(7129..7953)
                     /locus_tag="SCM56_001216"
     CDS             complement(7129..7953)
                     /locus_tag="SCM56_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEG1110031.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(7946..8506)
                     /locus_tag="SCM56_001217"
     CDS             complement(7946..8506)
                     /locus_tag="SCM56_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110032.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            8680..9636
                     /locus_tag="SCM56_001218"
     CDS             8680..9636
                     /locus_tag="SCM56_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG1110033.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(9941..10897)
                     /locus_tag="SCM56_001219"
     CDS             complement(9941..10897)
                     /locus_tag="SCM56_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEG1110034.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            11001..11603
                     /locus_tag="SCM56_001220"
     CDS             11001..11603
                     /locus_tag="SCM56_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEG1110035.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(11691..13190)
                     /locus_tag="SCM56_001221"
     CDS             complement(11691..13190)
                     /locus_tag="SCM56_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HEG1110036.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(13206..13730)
                     /locus_tag="SCM56_001222"
     CDS             complement(13206..13730)
                     /locus_tag="SCM56_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEG1110037.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            14028..14777
                     /locus_tag="SCM56_001223"
     CDS             14028..14777
                     /locus_tag="SCM56_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEG1110038.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            14768..15553
                     /locus_tag="SCM56_001224"
     CDS             14768..15553
                     /locus_tag="SCM56_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HEG1110039.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            15522..16238
                     /locus_tag="SCM56_001225"
     CDS             15522..16238
                     /locus_tag="SCM56_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEG1110040.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(16340..17374)
                     /locus_tag="SCM56_001226"
     CDS             complement(16340..17374)
                     /locus_tag="SCM56_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HEG1110041.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(17378..18436)
                     /locus_tag="SCM56_001227"
     CDS             complement(17378..18436)
                     /locus_tag="SCM56_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEG1110042.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            complement(18656..19804)
                     /gene="nagA"
                     /locus_tag="SCM56_001228"
     CDS             complement(18656..19804)
                     /gene="nagA"
                     /locus_tag="SCM56_001228"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEG1110043.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(19880..20716)
                     /locus_tag="SCM56_001229"
     CDS             complement(19880..20716)
                     /locus_tag="SCM56_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1110044.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(20900..22117)
                     /locus_tag="SCM56_001230"
     CDS             complement(20900..22117)
                     /locus_tag="SCM56_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEG1110045.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(22266..22445)
                     /locus_tag="SCM56_001231"
     CDS             complement(22266..22445)
                     /locus_tag="SCM56_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110046.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            22500..22583
                     /locus_tag="SCM56_001232"
     tRNA            22500..22583
                     /locus_tag="SCM56_001232"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22534..22536,aa:Leu,seq:gag)
     gene            complement(22823..24193)
                     /locus_tag="SCM56_001233"
     CDS             complement(22823..24193)
                     /locus_tag="SCM56_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110047.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELI
                     FSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLI
                     FLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLF
                     TELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLI
                     MGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFI
                     ITVINR"
     gene            complement(24907..25197)
                     /locus_tag="SCM56_001234"
     CDS             complement(24907..25197)
                     /locus_tag="SCM56_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110048.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(25405..26097)
                     /locus_tag="SCM56_001235"
     CDS             complement(25405..26097)
                     /locus_tag="SCM56_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEG1110049.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         7958 a         5443 c         4448 g         8528 t
ORIGIN      
        1 gtaaaatcta atgattttta atgcattatt cctttttgtt cgggtttaaa tagttatttt
       61 tattttctcc tatctaaaaa agcagcccac ataaaaagaa ggcatgacaa aaagcatgtc
      121 atagcccctt tttccgaata aatagtgttc aaaagacagc ctatcggcaa taaggcaaga
      181 ttttataaac tacgggaagt aatttccgta aatctattct tattctttta agctgacaag
      241 ctcgtcaatc actactgctt ttcctgaagc aatagaatga tttgctgcga ttcctgtcaa
      301 aatcgacaaa ataccatcta cgtgagttgc tgcccgattc cacggatctt cttctttttc
      361 ttcaccaaat agatcatcta ataaacgcgg atctcctccg ccatgtccgc ccgcttcttg
      421 ttcgacgatt acctcatatg gctcaccgaa catcggatag acatggatgg actgaagaac
      481 ggctgctcct tcatcctttt tatctccacc tgcattgatg taagactttt cgatcacttt
      541 catttcgatc cgtcctttgc tgccattgaa agaaacattg aatccttccc acggcatata
      601 cgcgtttagc gaatacgtca aaatcgcacc agtttcatat ttgaccgtta cgcctagtgt
      661 atcttcaata gagatatcat cggaaaatac actgcggtca cgaatatatc cactgtcttt
      721 ttctgcttct aaatacattt ctcgcaattc ctcattggct gacaggtcta tagcaaatgg
      781 atcagcttta gctagttcgc ttccatagca tcgactgtaa aatatttcta ctccgcgttt
      841 ctccgcatta tgcatcccat aaaagtttaa tccaccgaat gcaaatacct tttggggacg
      901 gctgttcaac caaaaattca ctagatcaaa atgatgggta gatttatgaa caagaagccc
      961 gccgctattg tgcttatttc gatgccatct tctgaaataa tctgccccgt gttgcgtatt
     1021 taacagccat tcgaagtgga tagaaaacac atcaccaatc acatcacttt ctatcagttc
     1081 ctttatcttc gtgtgatacg gtgcataacg ataattaaaa gtgactcgca ggcttcttcc
     1141 cgtctgtttt tggaccgtta ttatttcttg agctttttcc tcatcgatag tcagaggttt
     1201 ttcgcaaata acatcatatc cttttttcat tgcacgaatg atataatcat gatgcgttcg
     1261 atctactgac gtaataatga cttcagccgg ccgtacgtca tccagtagtt gctcaaattg
     1321 tgatggcata catgtcttta cttttttctg tccatacttg ttcacaagta tccgatttgc
     1381 ataatccatc cttgtttgag atacatcaca aaaaccgact agttcacaag tttgatgata
     1441 cgtagaggca attgcctcat aaaaaaaacg cgctctccca ccaagtccga cttgtacata
     1501 ttttttcttc atttaaagtc attctccttt tcttttaagc aaaatactgt gacaaatggc
     1561 agccctggat acagtaatgc agtgagtaaa aaatcaggct gtatacacca gaacagtcca
     1621 tacaaaaggc ataaatacca cagaaacccc ttgacgtttt ttgacaacag ccagatattc
     1681 cttttgatca aataaacaag actatctgct ttatcttctt tgcagatatc aattagcgta
     1741 ctaaaaaaac tatgggatac aaaaagcaac agacacaata gaaaaggaat cagataccaa
     1801 gctccaatct ttcccactaa taaataccaa tcaactgcaa aaataatgac aaacagctga
     1861 aacaacgtaa tgatgaaaca catccgtttg attttccctt tcctccaaca tacctttttt
     1921 tctgccaagt aatggacaga tagataataa gaaggataca tcatcaaaaa ataaatcaga
     1981 tagacaacac gagcagacag cactaatcct aaagtgaaca aacatcctag aactaaagcg
     2041 aaaagagcat tactgataag actggttaaa aaaatccgac agacatagta gttgagcttg
     2101 taaagaaaca tctcttctat tggctttttc atattcagcc cttcagaccg cttgtactga
     2161 tcccctggac aagatattta ttgaagaata agaaaatcag aaaaaccggt agcagcgaca
     2221 acgtagacat cgcaaacata ctcgcgtaag tcgtactaga tccagcgtct gcaaataatt
     2281 tgatggcaat cgataccgtg taagatctcg gcttgtttaa ataaatcaat ggtcccatga
     2341 agtcgtccca tttccaataa aactggatga tgatcgtcgt gatgatcgaa ggtttcagca
     2401 aaggaaaaat aattttggta aagacactat acttgctgca tccgtcaata atcgctgatt
     2461 catctaactc tttggggatg ctgaccatga attgcatgat ctggtaaatg aaaaatgctt
     2521 gtccaaaaaa atgtggtaaa atcagcggca catacgtccc aaccaaattc agccgattgt
     2581 aaataataaa ctgcggaatc aaaatcactt gtgcaggaat catcatcgtt actaacatga
     2641 cgacgaacca gaatcttttt cctttgaaat cgattctcga taatgcgtaa gcaatacaag
     2701 tcgagctcag aactgtccct atcgtcgaaa ctgcagagat gacaaacgaa ttcctaaaga
     2761 aagtaaaaaa tgaaattcca gcaaaccccg cccatccttt tgcgtaattt tcccattgaa
     2821 aagtctctgg aatcaaattc agcgaaccac tcataatctc ttgatccgtt ttaaaagaac
     2881 ctgcgatcat ccataagacc ggataaatca tgactagacc taatagcaaa ataaaaccgt
     2941 attgaaacca tttcgttact gtattattca tttcttcctc ctctcattca cacaacgacc
     3001 catttttttg atgatttgaa aatcagaaag gtgacagctc ccataatcaa cagcatgatc
     3061 cacgaaatag cactagcata ccccatagaa ttaaaagaaa aagcttggtt atacacataa
     3121 agagcataaa agttcgtggc atcattcgga ccaccttggg taatgataaa tgcctgcgta
     3181 aaagtcataa acgccgcgat tgtctgcata accaagttat acaaaatgat cggcgaaaga
     3241 agaggtaacg tgatcttgta aaacgtctgc catttgttcg ctccatcgat ctctgccgct
     3301 tcataatacg agcctgggat ctctttgata cctgcagcaa agatgatcat tgccgaaccg
     3361 aattgccaag cagacatcaa aacaagaggg atcatgacca gtttttgatc gctaaaccag
     3421 gaaagcgcag gtgcgcctaa tcgttccagg aaaatattca ctaacccgtt tttagaaaac
     3481 aactcccgcc aaactagagc gatagaaata ctgccaccga tcaaactagg cacataatac
     3541 aaagaacgaa agaaagtcgt tcctttgata ttcctcgtta aaagataagc aatcaataga
     3601 ccaaagatca gtttcacagg tacagatgca aatacataga gaaaagtgac tcggatggag
     3661 ttcaaaaaac gggcatcact cagcatcttt atataattat ccaagccgat ccatgagact
     3721 ggatttccca aacgataatc ggtgaacgaa taataaagtg acaagatcat cggtatcact
     3781 gttatcaata gaaatcctag gatgaatgga gaagcaaata cgtaaccgac cacattgtct
     3841 tgatccaaga accgttttag tttttctttt ttcatgcctt ccttctgctg ttacttcttc
     3901 ataaatgact ttttcaacta aaagattgta ttggtcttga atttccggat tcaaagcact
     3961 atcaaagaca ttcacttcgc cgttgtccat atcggaaatc atttctaaat aatcaaatgt
     4021 cgcttgcaaa gttgcatcat ctgcacgagc gagctgttct cgaatattcg acatgattgg
     4081 tacaccacgt tctcctttta aaatttcatt ggcttcttca ctgttttgaa agaaatcaat
     4141 aaacttagca gcttcttctt tatgggctga gctttttgcc atactcagca tttgcgaaga
     4201 acgtaaaggg ataccagatt ctccatctgc ttttctgcga ggtggattaa tcaactgaat
     4261 ctccatatct tcaggagccc ctgacaatat ttctgtcaac tggttacttg atagaaacac
     4321 cattgccgct tctctatccc taaccgcctg aaaagacaga tccgtcgtac ttgcagctcc
     4381 accacctggt tctggatggg cccctttgtc taccagattt ttacgcagct ggaaaaaatc
     4441 tgttagatag tctggatttt caaatcccaa gctgttagat agatcttccc gattataaaa
     4501 attcaatcct ttctcaactt gtggtatttg aataatacag ccagctataa aatcttcgaa
     4561 tcttgaaata gcatagattc ctaattcttc agtgatttga tagctgattt gttcaaattc
     4621 atcccatgtc caatttgaag ctggttcagg gatatttaat tgttcaaata tatttttatt
     4681 gtaagcaatc gcaaacgaat tcaccccgtt agacagtcca actaattgcc cgtcgtaagt
     4741 cgtcgttgct aaataggatt catctgcatt agaaatatca attatccctt gctccacata
     4801 ttcatctaat ggttcaatct gattgatgta ttgaggaaat tgattgttca gttggaaaat
     4861 gtcccagata tcatctgatg ctgctagtgt acctaatttt gtgtaatatc cttcataatc
     4921 ataaaattca taatcgatcg tcacatgggg atttttttct tcataaagct cgattacttt
     4981 tactgttgca tcgtgtcgga cttgcgaacc ccaccaagcc atgttcagcg tgattttttt
     5041 gtcatcttct tgcccacaag ccgtcaagaa gaataacata acacttaaag ctaagatgat
     5101 tctcattctt ttcttcattc tctgcacctc tacttccatt gttatttttc aatatactgt
     5161 atttttatac agaacatatt tattaaacga tctttttcaa ggggaataaa aaccaagact
     5221 tgccttatcg ttctgcctag gatgaacaac tcaatacttt caagaaaaat tagatagtgc
     5281 tgattcttct ttcatcgcta aaaacacttc ttgcttcaat gatgggagga taattacgca
     5341 aagaaaaaag gatatcagac aactgcccaa tatccttgac atagctaaat aagcatgctt
     5401 cttcgtgtgc gctcttccct aaaaaacaga aaaataacta gtaattctgt atgaaattag
     5461 gtacaatata cacgtcgatg cacaagcatt gactatggca acttgattgg tcagttcccg
     5521 ctgacttttc aagccccgac aatttagctg ctactaaatt gtcgggtgcc ttttttagtt
     5581 tttattcgtt tttcattcta tagaaagcgc ttcaataaat caagacattt ttttgttaga
     5641 atgctaataa taacaaaagg ttttaactct tcttatgtac attacaggta aaaaaacaaa
     5701 aaaatttctc ttttttttga attctgaaca aatagtactg gagtacaaaa gacgaggaag
     5761 agccactatc tgctcttcct cgtcttttga ttctcacctg ttattcatga ttctttttct
     5821 cagtttcccg atatttttcc attttacgac taaaaagctc tccgctactt ggtggcgtat
     5881 atgtgatggc atttgctcct gcttgaatgg tctcagcaat actttcttcc gttggaccac
     5941 ctgtcgcaat gataggaagt tcaggatatt ttttacgaaa atggcgtact gcttctgcag
     6001 tttctttacc tgcactgata ttgataatcg aaataccagc tgctaacttt tcatctattt
     6061 ctgtatattt cgacgttacc gtactgataa ctggaatatc aactactcga caaacttctt
     6121 ttactgtctc taaaggcgta ggaccgttca aaacgacccc taacgaacct tgcgcttctg
     6181 cgaacatgct catgtaggaa gaacgagcac cttgtgtcaa accaccacct acaccggaga
     6241 aaactggaat gtcagctgcc tcaataatgc ttttagtaat cgctggatga ggtgtaaaag
     6301 gatacacagc aattactgca ttcgcatttg tattacgtat gatcgcaata tccgtagtaa
     6361 agatgatcga acggatcaat tttccaaata tcttgatccc gcttgcttct aaaatgattt
     6421 ccggtactct tacgatatct tttctcaatt ccgtcatgat ttctggcgta aattgttctg
     6481 ccatcagcca cttctccttt gctatctccg gtcatactga ttcaattgat acgtctcgat
     6541 gacctggatg attttttgcg cataaccggg gtctgtcgca taacctgcat cttgcaatgc
     6601 ttgtgcggct tcctgatagt tggtggccgt aatcactttt tcatacagcg caggattcca
     6661 atctactcct tggacgaaca gttgtgtatg gtcatccatg gactgttccc atgaatcata
     6721 cactttgaag tctccttgga tagttatcca ttcttcgtta acaaattcct ttgtttccag
     6781 gcttactttc ttctgatccc cataagcctt gatcccgaaa agattattgt atttgctagc
     6841 caaggtactt tttccccaat ttgattctaa tatagcttgt cctaaaatga tactaggaag
     6901 cactccataa ccatcttgta gttcttgggc atgcggaaca agccgttcaa taaattgctg
     6961 atgggattgc gcttcatttt cttgctgatt atcagtaaaa ggagaagaca gattcttcaa
     7021 ggaaaaaaca aaggctaaga gtatcactgc tacaccggca accactgctg gccaacggat
     7081 attcttgcgc ttttgctttt tccacttttt tttcgccatg atcaattcct acttttcatt
     7141 taattcattc agatattctt ctagcgtcaa ttggtgtttg gtgatatact cagcgttttc
     7201 tttaccgaca taacgtaagt gccaaggttc atatgtgatc ttagttatat cttcgtgtcc
     7261 ttttggataa cgaacgataa atccatactg cgcagcatta tctgcgatcc atttagctcc
     7321 aggctgatct ccgtaacttg catccaatac agtagaagga taactgttgt accagttttg
     7381 atcgacgaca tcgactgcca gacctgtgtg gtgttcgctg tatcctggct ccgtaatcgt
     7441 ttctttagta atctttgtcg cctcctcttc tgatacacct tgacgactca tcacatcttg
     7501 gacattttgt tcgaaaactt gttgttggga tgatacagac cggtaagctg agaccatcac
     7561 taatggaaat ccagctgctt cagcagcctt ggcaaatgct tcatattctg gagcaatgcg
     7621 tttgtcgatc aagtatccgt tacttaatga tgccaattga gtagactcat caatttcttt
     7681 ttctaatttg tgatctggac caaccaaaac tagcttccaa tcgtctgttt taacatctgg
     7741 caattgtttg gacgtacgat cttttgtttc tttgtccttt accttgctgc tggttgtttg
     7801 actgttcttt tctttactag cagttgtttt ggtgcttgtt cgttcgactg cttgattgcc
     7861 ggcttgaaca tgcgaatcat catcgctaaa aataacatag cccattgctg cgatcataat
     7921 cacaactgct ccaaatccta aaaatttatt cacttgaaaa atcctttcgt tgttcttcac
     7981 gttccccatt cgcctcatga acgtaagttt cttccagatt ttcattcacc caatcgagaa
     8041 gttcgatttt ttctccttcg atctgttttt gaacatctgc tggcagacga aaattcaaaa
     8101 tatcatcata gccccattca tcatattgaa tacttacttt caagtcaagg tatctaccag
     8161 cctgctgatt atttttcaaa gggatcagct cgacttttgc tgctttgctg ctaagccatt
     8221 tttgcgcgat gcgtgttttg atttgcttgt actctgcaac ggcaacgttt cgtttttcgg
     8281 gaaaaataat cgtttgacgc aaaatctttt gcgttagcat ccattcataa tcagtgaaaa
     8341 gattttttac tttctgcatc gcttcagatg gcagattttt ctcgccatct ttccattttt
     8401 gccattgttc tggactgatc agcaagtatt catctaaaaa atttttttca gtttcaaact
     8461 gttctaataa tacactcata ataagatcaa tatatacttg atgcacgttt atcccctcct
     8521 tttaactatt ctacttaagt tttgatcaaa aagcacctct taaatcgctg ctttcatttt
     8581 ttttaatgtt tcaagtcctc tcctctttat tcttcgtgat ttttgttata ataatagaca
     8641 gacaagaaac tcaatccaac tatgaaggag cgttttcaga tgaaaaaata tcaatgggga
     8701 atcgttggtc ttggtaccat cgcacatgag tttgccgaaa gcttcaatca agagacgagc
     8761 gaactaacag cagttgcttc cagaacttca gaaaaagcgg aaaacttcgc tcaccgttac
     8821 aatattccaa aagcttacgg aagctatcaa gaaatgttag acgatgctga aatcgacatc
     8881 gtgtacattg ctgtaccaaa tagacagcat atcgaccata tcttggcagc attggaagca
     8941 ggcaaacacg ttctatgtga aaaagccatc acgatgaata aaaaagaact ggctgacgct
     9001 atgcgcttag cagaagaaaa gaatttgatc ttagcagaag cgatgacaat cttcaacatg
     9061 ccactttatc agcagttacg ctcaataatg gatacaggaa aactgggggc tttaaaaatg
     9121 atccaagcac ctttcggcag ttacaaagaa ccagacccta aaaaccggtt cttcaatcca
     9181 gaattagctg gaggagctct gttggatatc ggtacgtatg ctgtatcttt cgctcgtttc
     9241 tttttgagct ctcaacctga agtagttgcc tctacaatgg ttccttttga aacaggcgtg
     9301 gacgaacaat ccgtcactat cttgcgtaac aaagaaaatg aacttgctgc tgtcagcttg
     9361 acttttcagg caaaaatgcc aaaagtcggt atcgttgcct ttgagaacgg ttatgtgacg
     9421 attgctgatt acccgcgtgc tgatcgtgcg gagatcttat ttacagatgg tacaaaggaa
     9481 ttcatcgaaa gcgggaatac atctgaagca atgaattacg aaataaagaa tatggtacac
     9541 atgattgaag gtgacttgcc caatcgctct ctcttcttga caaaagatgt catcgaaatc
     9601 ttggatcaaa tgcaagttct atggaaaaat atgtaatttt aaaaggttgt gaaaaagctg
     9661 ttctgcatca agtaataaga acagccaaac aaaaatagct cctcctattt ttcgtttggc
     9721 tggacttatt accgcagagg aaagcttttg aacaccgttt aagaaagagg ttgtgagcct
     9781 accgttcagc tccgagtcac aaggagcaat ttcaaaaaac agcttctcat gtttgttgaa
     9841 tattgtgact tgtgaccgag gagttgacag gagaacaccg tttaaagaag aggttgtgaa
     9901 atcagctttt tgacctgaat attagctgaa ttcacaacct ctatttttgg attttttgtt
     9961 gggagaattc tgccttgatt tcaataaaac ttttaaaaga ctgcaaaaac tggaacaatt
    10021 ctgctcgatc ctcgaattca gggcgactag tcggcagagg catttgccga tacaatacat
    10081 aaacttcttc aatacgtgtt aaaatttgtc tgccatcatt tgactctgaa aatgtttctg
    10141 cagtataaat caataaacca tagatatgtt ttccataata agctggttgc tggattctct
    10201 ctagattttc cagcatgtct ttaatcacat tggcctgtgc tcttctcata gagaagtaag
    10261 tttcgtagta tagcggctgt ctgagccagt agttttcttg atgttctctg gcatcttctt
    10321 ggctttcgcg aatgaatgtc agtaaatgtt cgcatttgat ctgcacatct tctttatttt
    10381 ctgataaaag aaattctgcc atttgtctca aaatatttcg aaattctttt tcgatctgta
    10441 tttggttttc cttcagctgc ttcgtacgat ctggcatgta aacatttgca agcaaagcaa
    10501 atactaatcc aatcgccatt aatcccgctt cattgccgat cagctgccaa gacatgtttt
    10561 cctccactaa ataatgagtg accaaaaccg aatttactac tatcccttcg gttaatttga
    10621 agcgagaggc aagcgggatg aacaggaata aaaaaagact gaagccaaaa atagtatacc
    10681 caacagcaaa aaaactgaac agtgctgttg ccgtagctaa tataaaggct gcgattcgat
    10741 tcatgcccgt acgcaatgta gagcgcttgg tatttcctac acttaataat gcaatgatcc
    10801 ctgcagctgg ccaatacaac agcgataagc tacccgctgt gaccatcgaa aagaagacac
    10861 tgatcactgt tttgactgtt cgcattccta tacgcattta cttccccctt ttttatttta
    10921 attatacttg aataatgaaa aactagctat cagacaaaag tttaggtatg atagtaaaaa
    10981 gaccaaaaag gagaatctct atgcaatttg gccgttttcg actaggtatg cgtacgatca
    11041 aaagtgcctt agctgttttt ttatgtatct tgttttttca tttgactcaa cgaggaatcc
    11101 cgatgattgc tgcattatct gctgtttttt ctttgcgtca ggacttgact acaaccgttt
    11161 cttttggaaa gtctaggatc atcggcaata cattaggtgg tacattagct atcatttatt
    11221 tttttgtcaa aagctatttt cacaatgatt ttttggtaga attgatcttg cttcctattc
    11281 tagttattat tgtgatcgtc gtgtcagatg gtattaacaa taattccggt atcatttccg
    11341 caatagctac gttgttgctg attgcgttga gcatccctca aggcgaatcc gctttgtatg
    11401 ctatccaacg tgtactagat acttttatag ggacattcat tgcaatcggt atcaatttct
    11461 ttcttcgtcc tcctgaaaca gaaaagaagg aagaactatc agaagatttg gtcgaactcc
    11521 aaaagaaaga acgattgcta agaacaaatt tacaagagat agaagctaag atccaaaaac
    11581 aaaagaaaaa tagcgaggac taattttctt ttgcaataaa ataaaaaaat ggagagaaga
    11641 actttaaaaa gtcttcctcc atttttgatt gaacggtgaa gtgtactact ttattcttcc
    11701 ttcatcgagt tttgttctgc ctcttttggc gcttcataca tatttacttg gctagtagtc
    11761 ccacgtgtcg acaaattctc tacgatttcc ttcaagtcaa gtcctgttgt cgcttttagt
    11821 gtttcttgag tagaagaaag caaatttgtg gcataatttg tcactcggtt cgctccactg
    11881 gtttctcctg aactgcttcc tgtatcgaca acagagattt tttcgatatt tcctagcggc
    11941 tgtgctgctt cgcgcatcaa ttgcggtagc atatcgataa ccatactcaa gacagctgct
    12001 tcgccatatt ccttgaaggc attggcgatc ttttgttttg cttcggcttc tgcttttcct
    12061 tttgctagtg ctgcttcggc ttctgcttgt ccagctaaac gtgtcttatt ggcttccgct
    12121 tctgccagtg cttccacacg gaatcgttct gcttcggctt cagtcacttc tttgactttt
    12181 tgagcttgtg cttcttgttc tttagcgtac cgatcggcat ctgctttttt cttcacttcc
    12241 gaatcatatt gcttttcacg acgtgtgatt tctttttctt ccagttcgat ttgtttttgg
    12301 cgttctacga ttttaatctg catttcctgt tcgattactt gttgttgtgc tcgggcactt
    12361 tctaaattgt aggcttggtc tgcttttgct ttagcaacat cttgttcttg tttgtaggtt
    12421 gctaatttca gttctttttc ttttaatgat tctgcgatct ctgtttgtcg ctgtaattca
    12481 gctgcttgtg attctttttc tgcttgagct tttttgatac gcgtttcttt tagagcttcc
    12541 gcttctgcta tatctgcatc tcttttgact tgggcgattc ttggtttccc taaagagtca
    12601 agataaccgt ttttgtctct gacctctttg atagtgaaag aaacgatgat caaccccatt
    12661 tttgcaaggt ctacagaagc gacttcttgt acgctttgac tgaacttatc gcgattttga
    12721 tagatttctt ctacagtcat agaaccaaga atcgagcgca agtgtccttc taatacttct
    12781 cttgcttcat tttccagttc ttctcttgtc ttgcctaaaa actgttctgc tgctgtagca
    12841 atctcttcta cagatgaacc gattttgatg atcgaagtac catcacacat caccggtacg
    12901 ccttgttcgg tatacacttc aggggtggag acgtccaatt tactcgataa tagactgatc
    12961 cgattcgaac gttgaaaaac tggcaagacg aatgcgccac ctccacgcac aattttgatt
    13021 ttgttattgc tttcatcaat gtgcacattt ttgcttccta aataacttcc actgatgatc
    13081 aacgcttcat ccggttttgc tgtctggtac ttcgctacaa agacgatcag caacatcagt
    13141 aaaatcaaag ctgcgattgc gatcgcgata aaaacaactg gtatttccat ttaacacacg
    13201 ctcctttaag cttttatcat gctttcataa ggcatgacat aacagattct ttccttgatc
    13261 tcaatgatca atactttcgt tccccgctca gctgtctgtt cttgggggct gtaaaaacaa
    13321 gctggacgac taatggaacc ggtaacactt ttcaactgaa tttctcccat acctcgtaca
    13381 gggatcggtg tgatcacaac agctacttgt ccttccagag ttttttcaga agacgacatc
    13441 gttgattcgg catgtttcat cggcatcaat atatagaaat taagcaaaaa taccaacact
    13501 atcgatatgc ctccactagc aataaataaa aataaagatg attgatctgt taaacgctca
    13561 cctagaaaac caaataagct tgtgaaagtt aaccaaggga taataagcat cggatcaagc
    13621 gggccatcaa aatcgaatac atctccaaaa ataatcaata ggactgccaa tcctgcacaa
    13681 atcagcaatg tataccaata aatcgtctca agtgcgattc cccctatcat atacacgcct
    13741 ccttccattt ctacgtctat cataccaatg tttgaaaatt ctgtatagct tcttttaccg
    13801 actcagtggt gttttttata ggaaggttgt gtttatcgtt gacaatttgt taaatttttg
    13861 ttttcatgtt aaaattttca caataaattc tttcgaggta gcctatgttt ccttttttta
    13921 agaaaaaacc attaaagctc cagaacacga tcatccttgt cgtttgtttc tccacattcg
    13981 tttctttgat tgtttctgcc atattgatca ggaattttat tctttcgatg aaccgatcta
    14041 gtgtgtcaga taaaatctct agcattgcta aaatcgttgc caatgataaa ctcatccaaa
    14101 aaaagctgca gactggtgac ccgacacatg cgattcaagg aataacaaat gaaatcatga
    14161 ttgcatcgaa tttagatttt atcgttgtca tggaccctga aggcattcga tattctcacg
    14221 cagacccaag ttacatcggt cttcctttct caagtcatga agatgtgaaa aaagtgattg
    14281 gaggacaaga atttttctct gaaaaagaag gaatgttagg gttcggcttc cgctatttcg
    14341 tccctattta ttatgatcaa gagattattg gtgcagtttg tgttggctta acaaacaaca
    14401 cgattgacaa tcaagtcaaa gatggacaaa aaatgattgt tttttcattg attattggac
    14461 ttagcgtagg catcataggc gccgtttttc ttgcccaaaa aatgaaaaaa gtactgcttg
    14521 gacttgaacc acaagaaata gcagaacagt taagtgaaaa aacgattatc gaaaatgaag
    14581 tagatgaagg aatcttagcc atctcttctg ataaaactat cctcttgatc aatacacctg
    14641 caaaaaacat tttctaccaa acagagcagc tattggattt cgaagtagga aaaaaatcag
    14701 tgataatcta tatgacgttt tgtttaaaga gacgattgag catcaaacga ccgttcggac
    14761 tcaatccatg tatttaaatg ggatagaaac tatttctagc gttacacctc tttttagcaa
    14821 tgaccgtttt ttaggagcag tcgcaacttt acgaaatcga tcagaagtaa tgaaattaat
    14881 ggaggagctt ggggggacga aacaatatat aaactccatg agagctcaaa cccatgaatt
    14941 catgaataaa cttcatgtca tttctggttt gattgatttg aagaagtatg aagaagtcac
    15001 ccattttatc cagcagctaa atcaaaatta taaaaaagag attggccaag tctcaagtct
    15061 gatcaaagta cctgcaattg ccggtttttt attaggcaaa ataaacgaag ccaaagaaca
    15121 atctgttgaa atgctgttag aaagcgattc ttttatccct aaaatggaga tggatgactc
    15181 catccattca cttttgcaag tgttagggaa cttacttgat aatgcaaaag aagccgtgct
    15241 acaaaaacat gaaagaaacg gacaggtccg gattcattta aactatgaca cagaagctga
    15301 agtattcatc gtgaccgtct gcgatggcgg tgtcgggata tcagaagaaa tctcagaaaa
    15361 aatatttacg ctaggttttt cgacgaaggg aatcaatcgt gggtttggat tgaatctcat
    15421 ccattcaatc gtcaaacgcc acggtggatt tttagaattt tccaaacaaa caaatggcgg
    15481 tacaatcgtc tatctagaat taccaaaaaa ggaagatcaa aatgatgaca aatgtcctga
    15541 taatcgaaga tgatccaatg gttgcagaat tgaatcgtac ttttgtggaa agcatcccta
    15601 atatgaacgt aatcgaaagt ttcagaaatg tagaagaagg aaaaaaatat ttcagacaaa
    15661 aaaacgcaga tctgctgctg cttgatgtat acatgcctgg cgaaagtggt atccattttt
    15721 tatcttggct tagacagcaa ggaaatgata tcccagtgat tttgatcact gctgcagatg
    15781 atatcgaaac cataaagaat gtgatccgct acggtgcctt agattatttg atcaaaccgt
    15841 tcactttgca acgttttcag ctggcgatcg aaaagtatct tgctttctct cagacaatcc
    15901 aaacgactga aaaagccgat cagaaattga tcgatcaagt attcaaccac accactccca
    15961 ttcacgaaat accatcattc atcgatttgc ctaaaggtct gtcaaaactg actttaaaaa
    16021 aaataagtgc atttattcta aaagaagata ctattttttc aacaaaagaa ctggctcaaa
    16081 aaagctcact gtcacgtata tccgtcaaga aatatttgaa ttttttagta gaacaagata
    16141 tattgaacga aagaatcaaa tatttggaaa tcggccagcc gctaacctgt tatgaattga
    16201 caaagaatgg gcggaaaaag ctcacagatt actgttgagt catcgaatta tcccatacaa
    16261 aatagtccaa gtatacagaa gtaacaactg tttacttgga ctatttttaa acattttatt
    16321 gacgaatatc catgttttct tatcttaatt gacgggcttt aggaagagat acctcatttt
    16381 tcgtttttcc agtttctatt atttcacgaa agttctcgaa acttgtcgaa atcatatttg
    16441 ataatgcctc atctgtattt gaacctacat gcggggttac aagaactcta gggtacattt
    16501 ctactaattg ctggatagca gggtctggaa tcgtctccca tggcttgaag gatcggaaaa
    16561 aaacctctgt ttcatttgca aaaacatcgg ttgcaaaacc agctaaatga ttgcttttca
    16621 aagctcttaa aatagcttca ttatcttgaa gctctccacg ggctgtatta atcaagatgc
    16681 tacctttttt catttgagct aaaaatcctt cattgatcat ttgatcgttt tcacctggga
    16741 tatgaggtac atgaatactg acaatatcgc ttttggctaa taactcttca agcgagtgga
    16801 acgtcaatac ttttttagca gcttctgtct gatgaatatc gtagccgatc actttggctc
    16861 ctagtccctt aaacaaaaca gcttccgtca accctatcct gcctgttcct atgatcccta
    16921 ctgtgcaatt tctgatttca cgactaaaca tttgctcatc cactcgaaaa tctttgaaag
    16981 atgttcggat cgtcgtatac gcagtatttc tcaaaagcat catggcaaaa gtcaaagata
    17041 actctgctat cgcatttggc gaatacgatg gtacacgggc tactgtcata ccaaaatcag
    17101 ctgccgcttg tagatcgata tgattgactc ccacagtccg tgtaaaaaca taacgaatcc
    17161 catattcctg catttttgca agattggtct catctgctat acaatttccc ctcaacaata
    17221 cagcatcatg gtcctttgct gacacgatat tgtcatgtgt caacagttct tcataaagac
    17281 tgagatcata atggtaaata ttcaattttt caaaaaaatc tatctcgttc ggacgaacac
    17341 cgtaacatgc gattctcata ctttcttcct ccatttctta tgaaaataaa cctatgccat
    17401 gaatcatttg taatacaacg atccatacag gtgtcagtac cacagcaaaa atagttgaca
    17461 gtaaggatgc attggaggct aaaagtgcct ccctatcaaa actgatagca taggctgctg
    17521 ccactgttgc agctggtgta gccatcatga taacgattgt tgccagtgct tcaaaattga
    17581 ctggtagaat attcgttgcc gtcaaaactg ctagaatcac gatattcaat gcaggtacaa
    17641 ataccacttt gactagactg taataccatg aagttttttc agatgcagct tttttgaagc
    17701 ttacttcacc taatgttgcg ccgattgcta gccacgccaa tggtgaactt aaaccagcta
    17761 aataatccat cggctgaaat agccacggtg cagtctggtc aattcgcaaa aacgctactt
    17821 ggcttatctc tccttctgca cctgtaactg acacttgtgg cagataccct tggaacagcc
    17881 aaatgaaaag gccggcaaaa gtagcgataa tgattggatt gacgaacatc gctttgatat
    17941 ttttagcttc catcttcaag ccactcattt ttatgtatcc ataagaatac aaaaagattc
    18001 ggtagccaat attgaaaatt gaggaataca tcacgccgac aggaccataa atcgcactga
    18061 cgataggcgt tccgaaaaag gtcgtagaac caaagatcgt caggacacgc aacgttgttt
    18121 gcttatcccc tttatagctt aagaaaaaag gttttgaaac tagaataaga ataatataaa
    18181 taactaatcc ccaaatcaag acattgattc cttgcatcag taattctgta ttgatatctt
    18241 gcatgaaagc tcgaaatgcc agtgctggca aagccactgt taagacgact ttcgatagaa
    18301 tttttccaac agattcagaa aaaatcccct tttttctaca aacaaaccct aataatataa
    18361 taaaaactgt tgaagtgatt gcactgacaa tcgacatatc tgtcaaagtc gatcgaaaaa
    18421 tgtcaaataa gttcatcttt ctcctcctaa ttatgttcat ttattcacaa taaaaaaagc
    18481 ttttattttg ctaatacgta ttaacatttt taattttatc tttacattgt tacttattga
    18541 acaataggaa attaaagaaa cttttgaaac ttatgtaaag tgatttttac tagaacacaa
    18601 aaaaacctta aaaccaaact taagaagttt agttttaagg ttaacttaaa ttatattatg
    18661 cgtggaaacg ttccacgcca tctagataag ttgcttccaa tgtcatatca gggttcaata
    18721 cgataaagtc tgcatcgcgt ccttgagcga tttttccaca tgtatcatcg attttacagc
    18781 tgattgctgg aatcagacta gccatcatga ctgcttgttc tggtgttgct aatccccagt
    18841 ctacaacatt tttaattgct tctttcaatt tcaatatact tccagctaag ttgccagatt
    18901 ctagacgtgc tgttccttct tttactacta ctggaaattc gcctaaattg taatttccat
    18961 caggcatccc accggccatc atacagtctg taattaatgc gacatgatca tgtcctgctt
    19021 tttccattag gattcccgca gcatttggat gtacatgatg gccatcacaa atcaattcag
    19081 aaaagacatg ttctaaagat aataacgcac ccaccatacc tggttcgcga tgattcaatc
    19141 cacgcatacc attgaaagca tggacaaata cacttgcgcc tgcttcaaca gctttttgcg
    19201 cttcttctag tgtagcatta ctgtgtccta gagaaacaac tactccttct tcagtcactt
    19261 gtttgacaaa ctcttctaca ccttgacgtt ccggtgctaa agcaattttt ttgattaaac
    19321 cacctgaagc ttcttgccat tcatggaaag tatccaaatc aggatccccg aaataacttg
    19381 ggttttgtgc ccctttgtgt tcttctgtga agaaaggacc ttcaaaatag attcctttga
    19441 ttttagctcc tggtgcttcc tgatacattt tgccgattgt ctcagctaca tcttttaaac
    19501 gttctttgct tgacgtcaat gttgttggta aaaatgaagt cactccacaa gataacagac
    19561 cttcagacat cactttgatg ccttctgcat cattatccat tacatcatga ttcatatagc
    19621 cgtgaatatg tgtatctacc aaaccaggag caatccattt gccgctttgg tcaatgatgt
    19681 tgacttctcc ctcaggtagt tctttggtgt attcaccaaa aagtccatcg gtaatttcaa
    19741 gatagcctgc atgtttgaca cctgacgcta aaaagaattt gtcagcatag ataaaagttc
    19801 tcattctatt ctctcccttt gtttttctac ctctaaagta ctcttattta gagaagaagt
    19861 caagaatggt ctacaccatt tacaaatttt ttgaaatttg tttcacagct tgtgctaagc
    19921 cttcatagaa tgctgctctt tctggttctt ctttacgctt gtatattaag cttttaagca
    19981 caaataaatc attgaggtaa tagtagctat tcttctcttg agcccaatag attccttgat
    20041 tcacctgttc taacgcttcg tcgatttcat tttgatggaa taagaaattt ccataattat
    20101 aaaaaatttg cgaaaattca ctttttgatc gttcatcagc tgattgatgc agcagttgga
    20161 tgcttcgact caaatagtag cgtgcttcag ccgtttttcc tgccacacca tatatttttc
    20221 cgatacaact aatcagttgt atttctgtgt cagaaacatg gattctttct ttttggctag
    20281 agtagcttaa tgcttctttc aaataataaa gggactgaga gatattttga cgcaacgtga
    20341 attcgcaaac accgagataa taataatagc gttgaaagtc agttgcttga tacagatttt
    20401 cagaaatatc agaagatttt agtacttgtt ctaacttccg ataatcacgt ttttggaaac
    20461 aactatccaa cagttcaaat aatttatcgt ttgtccgagt agtttttcga gagagattca
    20521 tcacatgatc aatcgttacc cctagccgtt gacaaatctg catcatcacc aatacattcg
    20581 gcagttcctc attattttcg atccgactta atacactttg tgaacaaata tcatgcgaaa
    20641 gcattttttg cgtcattttt tgttcttttc gaatctcttt aatgatcgtt ccaaaggact
    20701 cgattaacgt ttccataagt atcctcccca aaatatgcgt attcgcattc tattcgtatt
    20761 ttactttagg aagtcgcaaa cttcaagaaa agacttgcaa ctaaataata tactttagtt
    20821 atttaagaaa actccactag aaagagagcc tcagtgtgtt tgtctgtggc cctcctactg
    20881 aaaaatttgg tttttaaaat catttttggc gataggttgc taggaattct ctcattttct
    20941 tagctgtttg tttcaaatca tccattttag gaagataaac gattcgaaaa tgatctggct
    21001 ggttccaatt aaatccacca ccatgtacaa gtagaatatg atgttcatgt aaaaagtcca
    21061 aaacgaattt ttcatcatcc aaaatattga atcgctttgt atcaattttt gggaatatat
    21121 aaaaagcagc ttttggtttt acggctgaaa gaccaggaat atcattgatt gcattataga
    21181 tatattctct ttgttcatag atccttccgc ctggcaggag cagctcatcg acactttgat
    21241 aaccgcctag cgcggtttgg ataatttgct gagacaatac attggaacac aaacgcatgg
    21301 aggacaacat attcagccct tctatatagc ctttgacgtg tgacttatct ccacttagga
    21361 ccatccagcc tactcgaaat cctgctacac gatgagattt ggatagaccg ttcagcgtaa
    21421 caacgaacaa atcaggcgct aatgtagcaa tcggaatatg ttctagacca tccatcacta
    21481 agcgatcata gatttcgtcg gaataaataa tcaggttatt ttgtcgagct acttcaacga
    21541 tttcttctaa taattctttt ggataaagtg ccccagtagg attattagga ttaatgataa
    21601 caatagcttt tgtccgactc gttatttttg atttgatatc atcaatatca ggataccatt
    21661 ctgcctcttc atcgcaaata taatgaacag gtgtgcctcc agctaatgca atagaagccg
    21721 tccacaacgg atagtccggc attggaacca gtacttcatc tccattgtcc agcaacccct
    21781 gcatacacat cgtgatcaat tcgctcactc cgttgccggt gtagatatca ttgatcgtca
    21841 cattcggaaa atgtttcagt tggcaatatt gctcaattgc ttttctagca gaaaagatcc
    21901 ccttggaatc tgagtagcct tctgagtcac gggcattcat gatcaaatca cgaatgactt
    21961 cgtttggtgc ttcaaatcca aaaggtgcgg gatttcccgt gttcaatttt aaaatactaa
    22021 tgccctcttc ttgcatccgc tccgcttctt ctagaacggg accgcgaaca tcgtaactga
    22081 caccttctaa tttgttggat ttttcaaatt ctctcatcac ttagccaatc cctcatttca
    22141 aaaagttaga actttccaaa ttttttgtta tttactattt caatatcata ctccatccta
    22201 gaaaattttg ccacttattt aggaaaaacg taagtattaa aaaaacacaa acaagcgttg
    22261 agaacttaat tgtcatgata gtcattccat agattcgctg cccaatccat tgcaattcca
    22321 caaatgattc ctagcataaa accaagcaaa aaacgtccga atgcaagtcc aatcaataca
    22381 cctaagatca ttcccagtcc tgaaaattct ccgtcatatt tctccatctt aataagtcct
    22441 cccatttttg agcttgacaa ctatgtaaaa cctcgctatc attagtgtgt tattattacg
    22501 cagatgtgtt ggaattggca gacaagcatg attgagggtc atgtgagcgt tagctcgtgt
    22561 gggttcgagt cccatcatct gcactacaac tcgtgaagtt aattttgctt cacgagtttt
    22621 tttgtttgaa cgtttcttta tgccattata tacatatcac tgtatccttt acaaaaagaa
    22681 cgtacaatca tgtaaacagc ctgaggtgag tatatgaagg tcggaaacaa gctactcgta
    22741 gaaacttgca agtgttaaaa caattaatcg aaaaacgagt aaaatccctc acctaatact
    22801 aaagcgaggg attttttgta tattatctgt ttatcaccgt tattataaag gtacaaatac
    22861 caagcacggt taatccaaat tgcataaatt catttttttg tacactcatg cttttcatta
    22921 gctctttaac tgccattagt actaagatgg cgataacaac ataaagtata tagtacgtcc
    22981 gcattccgga agcccatact gtgggagaaa atcccataat taaccttgaa caaaatccca
    23041 ttatcatcaa aataactaaa aaaatagaag ttttcttatt gtcaaaactt aaataaattc
    23101 caacaattaa acatagtaat aatgccaaga ctactaaagt agctatccaa gtacctggat
    23161 aaaaaagtga cagtttcgtt cctaactctg taaagagatt attcaggtta ctactactcc
    23221 aaatcattgc ccttttattt ccattgacgt aagtaaagct ttgacccatt gtattaccaa
    23281 aataaataat caggtttaag aaaaacggta atgcagtcaa tatacgcaca taatagtttt
    23341 gacatttcct atatgttaga aggaaaatta agaaaaatag caataaaaat aagatattca
    23401 tgtctaaaaa aaacggcttt ccaaatgatg aaatacctaa atctaactta ttaagaaagt
    23461 tgaaattttt atattcagga aaccatttat taatttcatg tgcagcacgt aatgcattcc
    23521 ctggagttgt caaagagaaa attaattctg ctaagcttat tattgaaaat actgataact
    23581 tataattata tcgtccattg acaatcaaat ataaactaac tatacttgtt agaacaaaaa
    23641 aacaggcgtt gacttgttct tgattagtta aaaatatcaa taaaggaata cttacccaat
    23701 atatttttct atttatttct tctcctctta agagttgaaa aaacggataa aaagttagta
    23761 aactaaatgc tactggccac tgatagttca aggtggttgc tctccaaccg gtttccccca
    23821 ttactgtcaa tggtattaaa cagtatatag agcaaaccaa taataaattt ttaacattta
    23881 ttttctcaaa aacatacttg caaagtaata ctacaattcc taacataatc gtagaattta
    23941 ataatttcca tagcataaag tgtttagaaa agaacattaa gaatagttct ataataattc
    24001 ttgaactcca tgtcttataa tgttctatcg aagcacccaa atagccaagt tcatctaatc
    24061 tacttgcata aactacgtca tccgctctca tagtcggcat atacatatta aagagtaaaa
    24121 agatacccca aatccctatt agtaacaaga tcagttcttt gttattcgtt atttttctat
    24181 tatgtttgtt catttttata ctccttctta tatttcctaa tagagtataa catagcttta
    24241 agcattactt aaagataact taaaagttat cttttaaata cttaattgtt aaatacctta
    24301 tttgatgcaa aagaaatcta cgatagtcaa caaaaaaatt ttcattatat ccagaaaaat
    24361 ttttcttagt taaggaccta tggattgcag taatgcagaa ttcgtcgaat aaactgccaa
    24421 agacatacaa tcatattgct tttaaaattg acgaacaaga tatagattcg tttgtctcaa
    24481 aaatccaaat gttaggttta actgtcgaac caggacgttc tcgtgtgaaa ggcgaggctt
    24541 cttcaattta cttttacgac tatgataatc acctcttcga gttacatacc ggtactctcc
    24601 aggaacgttt aaaatcctac aattccacta cctgagacta gtaatgacta gtctcttttt
    24661 atggcctctg atccccccat tgagcgaata ctaatagggc ttcgaattga tctgttcaat
    24721 ctttaagcaa tatccctcaa atgagtttgg aaagtttagt gattcatcac aaatacttaa
    24781 atacatgtag ttatatatag attgaggttc ttgtgagcgt atgctcgtgt gggttcaaat
    24841 cccatcgtct gcatccatct ttccaaaaca agcggctagg acatttttgt ctcagccgct
    24901 tgttttctag tttattcttt catcttgtcg gacctttttt cttgggtata gacttttctc
    24961 atattctacc acaattttgg gtttccctaa cacatgatag gatctcacaa caaattgctt
    25021 tctattatta taataacctg taacgactaa cttcatccct tcgtctactt cagctaggaa
    25081 gttcaaacta tgggaagcaa tcaaacaatt gaccccattt aaactgaaac ggatcaacgg
    25141 tctggcacta acttgcaaag tacggatctt atctactgtt ccatattttt tctccataac
    25201 cttcacctca ataccattat acgaacatac attccctttg taaagaagaa actctgtttt
    25261 atctttttga ctttttgtct attacctttc ttaaaatcat attggcactt cctgcgaaga
    25321 tgaaggacat gtactaaaaa aaagaggcag cagtatagat tactgttgct tctttactaa
    25381 tttaggttta cttgttaaga aagattattt acgcaaatgt tcttcaacaa tttgctgaga
    25441 tttatcagct gtatcttcca cttcatcttt tgctttttct aacttttgaa tatattgttt
    25501 caagcttgag ttttcagcag ttgcttcgtc taatttcttt tgtgtatctg ttaactgagt
    25561 ttctacattt gttttttctt gtgtcagtgt tgcgatttgt tgattcaaac catttacagt
    25621 attttgtaat tctgaaagtt ttgcttgtgc ttcttgtagt cttgctgtcc aatctgcatt
    25681 cagatgattg atttcatcat ttttttggtt gatctcatct agtttgacat tgatttcatt
    25741 ttgtttgctt tgtagttgtg catccttgct gactaattga ctttctaagt tttgtttttc
    25801 tgctgccgat tgattttttt ggttttctaa ttcatttact tttgtttggt attcttggac
    25861 atctgccttt gaatcttgca atgtctgttc tttttgtgct agtcgagcgg taagatctgt
    25921 aagcgcttgc tctttatctg tcaatgtatg atcaagctgt gtcagattct cattgattgt
    25981 atcaatatct gtctctccac cccacaaaat aatttcattt gcgtaccgcg tgcctgtgta
    26041 gccaattaga catagaaatg caacgacaat cccaccagta ataaatttct ttttcatttg
    26101 catagctcct taatccaata cttttacatg ctaaaagtaa gtgatctcaa acacaatgtc
    26161 aagcgttttt aaattacatg tgtaatttta atataaatac tatcaaatac tcatttaatc
    26221 ctcctttttt atctatttaa aaaatctttt ttatagagaa aaatgcaggt taatactttc
    26281 ttaaataaat atttatggtg aacaaaaaca aaaaagcaag ttttattaaa cgtaaccctt
    26341 ttcatttcat agattactgt atttattaca aaaaacg
//
LOCUS       DARBDV010000031        26222 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000031 DARBDV010000000
VERSION     DARBDV010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.031"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(144..308)
                     /locus_tag="SCM56_001236"
     CDS             complement(144..308)
                     /locus_tag="SCM56_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110050.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            603..1931
                     /locus_tag="SCM56_001237"
     CDS             603..1931
                     /locus_tag="SCM56_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEG1110051.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2133..2966
                     /locus_tag="SCM56_001238"
     CDS             2133..2966
                     /locus_tag="SCM56_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEG1110052.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2982..3719
                     /locus_tag="SCM56_001239"
     CDS             2982..3719
                     /locus_tag="SCM56_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEG1110053.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3874..4842)
                     /locus_tag="SCM56_001240"
     CDS             complement(3874..4842)
                     /locus_tag="SCM56_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEG1110054.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5074..5907
                     /locus_tag="SCM56_001241"
     CDS             5074..5907
                     /locus_tag="SCM56_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEG1110055.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5941..6780
                     /locus_tag="SCM56_001242"
     CDS             5941..6780
                     /locus_tag="SCM56_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG1110056.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6806..7366
                     /locus_tag="SCM56_001243"
     CDS             6806..7366
                     /locus_tag="SCM56_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEG1110057.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7565..9286
                     /locus_tag="SCM56_001244"
     CDS             7565..9286
                     /locus_tag="SCM56_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEG1110058.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9451..10593
                     /locus_tag="SCM56_001245"
     CDS             9451..10593
                     /locus_tag="SCM56_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEG1110059.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10609..11820
                     /gene="thiI"
                     /locus_tag="SCM56_001246"
     CDS             10609..11820
                     /gene="thiI"
                     /locus_tag="SCM56_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEG1110060.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            12393..13040
                     /locus_tag="SCM56_001247"
     CDS             12393..13040
                     /locus_tag="SCM56_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEG1110061.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    13169..13385
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13433..16078
                     /locus_tag="SCM56_001248"
     CDS             13433..16078
                     /locus_tag="SCM56_001248"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEG1110062.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            16388..17701
                     /locus_tag="SCM56_001249"
     CDS             16388..17701
                     /locus_tag="SCM56_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEG1110063.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            17691..18344
                     /locus_tag="SCM56_001250"
     CDS             17691..18344
                     /locus_tag="SCM56_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEG1110064.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            18399..18950
                     /gene="radC"
                     /locus_tag="SCM56_001251"
                     /pseudo
     CDS             18399..18950
                     /gene="radC"
                     /locus_tag="SCM56_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            19081..20166
                     /locus_tag="SCM56_001252"
     CDS             19081..20166
                     /locus_tag="SCM56_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1110065.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            20163..22055
                     /locus_tag="SCM56_001253"
     CDS             20163..22055
                     /locus_tag="SCM56_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1110066.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            22062..22439
                     /locus_tag="SCM56_001254"
     CDS             22062..22439
                     /locus_tag="SCM56_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1110067.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            22593..23375
                     /locus_tag="SCM56_001255"
     CDS             22593..23375
                     /locus_tag="SCM56_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HEG1110068.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(23501..24232)
                     /gene="erm(A)"
                     /locus_tag="SCM56_001256"
     CDS             complement(23501..24232)
                     /gene="erm(A)"
                     /locus_tag="SCM56_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HEG1110069.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(24290..24349)
                     /locus_tag="SCM56_001257"
     CDS             complement(24290..24349)
                     /locus_tag="SCM56_001257"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HEG1110070.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            24741..25403
                     /locus_tag="SCM56_001258"
     CDS             24741..25403
                     /locus_tag="SCM56_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEG1110071.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            25638..25781
                     /locus_tag="SCM56_001259"
                     /pseudo
     CDS             25638..25781
                     /locus_tag="SCM56_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            25931..26131
                     /locus_tag="SCM56_001260"
     CDS             25931..26131
                     /locus_tag="SCM56_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG1110072.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
BASE COUNT         8989 a         4198 c         5412 g         7623 t
ORIGIN      
        1 ccggagtaaa taaagcttat gcaaagaaaa agaaatccaa tcacttgggt ttctttttct
       61 tttattatta tgatgaatca agctttctgt ttttgtcacg tatgttgtaa tctttacgaa
      121 agagtagagt tggctactca taatcaacca tacttcccat catcgctaaa tacgaaataa
      181 atcagcattc ctacaccaat caaagcacaa gctgcaccaa gaaaagggat gacattccca
      241 aagttatctg tattcataag tatgccacca cccacaaaca aaaagccgcc agcaatcagg
      301 tttttcattt tattcatctc ctttcttacg ttatagtatt tccttctttc aatgagtatt
      361 ttgaaagcta tcttattgta tgatgttctt ctaggcctct tctttattat acaggccatt
      421 ttcgttatct catagaaata ttccttttta tctgaaaact attgtatacc gtatgacatt
      481 tttcttcatg aagttgtaag tatattctca ttcaaatgaa aatttcatca tagaagagat
      541 tgtatttcta aattcttaag agtaaaatac gaagtataac ttggatttgg aaggatgatt
      601 ggatgaaagc tattacgcct gatatgacaa caaaatttaa caaacagttt caagcaaata
      661 tcaaacagca ggcattgcag cgggcagttg tgaagaacgg aattactgct tctgctgaaa
      721 atgtttcagc acatgtggag aatacaccgg tattttctat agatttggct acggggaaag
      781 tggctaatca aatgcaatcg gggcgttgct ggatgtttgc tgcactgaac acattccgtc
      841 ataagatgct gaatcaattc catttaaaag agtttgaatt atcacaaaat tacacttttt
      901 tctgggataa atatgaaaag gcaaattatt tctatgaaaa catcattgca acaggaaaag
      961 aggcaatcga tagccggaaa gtagctttct tactcgctac tcctcaacaa gatggcggcc
     1021 aatgggatat gatcgtttca ttattccaaa aatatggcgt tgtgcctaaa tcggtgatgc
     1081 cggaaagtgc gaatagttca aattctcgag atttgaataa ctacttgaac aaattactgc
     1141 gtaaagatgc agtagtgtta cggaaaatgg ttgctcaagg agaaacatta gacgctattg
     1201 aagaaaagaa agaagaaatg ctagaaagca tttataactt cttagctatc tcattaggaa
     1261 cacctccaaa agaattcgat tttgagtacc gcgatgaaga aaagaactat catcttgacc
     1321 gaggactgac accacaaatt ttttatgata aatacatcgg tgtaaaatta gatgattatg
     1381 ttagcgtgat caatgcacca acaaaagaca aaccattcaa tcgttcttat acagtagaga
     1441 tgctaggaaa tgtcgttggc gggaaagaag tcaaatatct gaatgttgat atggaaactt
     1501 tcaaaaaatt agcgattgct cagcttgaag aaggagaatc tgtttggttt ggttgtgatg
     1561 taggtcaatc ttctactaga actagtggca tcatggcctt agatgcttat tcaatggatg
     1621 atctatttga tacggatttt acaatgacta aagctgaacg tttagatttt ggagaaagtc
     1681 tgatgaccca tgcgatggta ttgaccggtg ttgacttagt agacgggcaa tcaacaaaat
     1741 ggaaagtcga aaatagctgg ggcgaaaaag ttggaaagaa tggcttcttt gttatgagtg
     1801 atgcctggat ggacgaatat acgtaccaaa tcgttgtaag aaaagattta ttgacgaaag
     1861 agcaattaaa tgcgttcaat gaagaaccaa ttgttctatc tccgtgggat ccaatgggtg
     1921 ctttagcata gacgagtatt tcaatgaaga gtatgtagta aagataaaaa gagctggctt
     1981 aagccagctc tttttatctt taaagtccat tccctgtttt ttatggtccc tgtgctataa
     2041 ttctaaataa aggataagga ttggaaataa tccttttatc attaaaaata tgatgacgaa
     2101 gtagaataaa attgggagta ggggggaaag tcatggatat ccaaactgtt ttatctgatc
     2161 tgaaattaaa tggtaaagtg attccagttg ttggtggcga tgttaatcag acctatcgta
     2221 taaaaaccga acatcgtgct tattttttaa aaatccatcc gaatgtaaaa aaaggatttt
     2281 ttgaagcaga agtagacgga ttaaaagaac tgtcagcatt cgtacgtgta ccggatacgt
     2341 atatgctagg tgaaacaagt gaaggcgctt atttgttaat ggaatggatc gagccgggaa
     2401 aaggggatca acgagatctt gctgcagctt tagctaatct ccatcagcaa acagcgccac
     2461 aatttggttt tcgtaaagat aactatttgg gtacacttgt tcagaaaaat tcttttgaag
     2521 aagattggtg gacttttttc tttaaagatc ggcttgaatc ccagatttct ttggcagaag
     2581 aaacgaatcg atggaatgta cagcgtcaag aaaagtatct gcgatttaaa gaacgtgtat
     2641 tgaaatcggt tgaaccaaaa aagatcactc cacgcctctt gcacggagac ctgtggagtg
     2701 gtaatgtgtt ctttgaccag caagggcatc cagtttttgt cgacccagct gtttcttatg
     2761 ggaatcggga acaagatatt gcaatgagcc agttattcgg tggtttccga ccggaatttt
     2821 tagatgctta tcaaactata tttccattag aaaagggatg gaaagaccga ctgccgattt
     2881 accagttata ttatcttttg gcacatttga atatgtttgg agaaagttac ggttcccaag
     2941 tagatcaatt actagaaaat ttttaagagg agggcgtgtt tttggcaaaa aaatatacca
     3001 aaaagcatga agtttcatat tatgagtgtg acatcaacca aacgatgaca tttccttcga
     3061 tgttaggagt agttatcaaa acgtctgaag atcaaagcga ccagttggga agaggcagtg
     3121 aattcgtcaa tagctttgga cttacttggg tcatcacgaa ttattccatg gagattaatc
     3181 gtctccctag agtcggtgaa gtaatctcag taacgactca agccatggag tacaataagt
     3241 ttttctgtta tcggaacttc tggatccatg atgaagatgg gaaagaatta gtcaaaatag
     3301 aatctgtttt cgtattgatg gattttgtga atcgaaaaat gagcagtgtt aatgaagaga
     3361 tcattgctcc ttttgagagt gagaagatca agaaaatcaa gcgtcaggaa aaaattgaaa
     3421 agattgattc gggcttcatg ttgccatatc gtgttcgttt ttatgatatc gatagtaatc
     3481 agcatgttaa taatgcgatg tattttaatt ggatcattga cgtattagga tatgactttt
     3541 tgactacgca tgttccagaa aaagtaatta ttcgatttga taaagaagta gagtacggga
     3601 atgagatcga aagccatttt gaacaaatcc ctatcgaaaa tggtgtcaaa acaaaacatg
     3661 aaatccgaat gcaagaacag ctgtattgtg aagcaaatat tatatggaaa aataaataaa
     3721 aaagaggttg tgacagaagg gggcaacttc aagaaataag aagaaattta cgaaaattgt
     3781 ttctcaaatt tttgtaaatt ttcgacttag ttttcgaaga agctattttt gtcacagcct
     3841 ctctttttaa gtttagctat tagataatga tgtttagttt tggataaacg aaatcaattc
     3901 ttcacccgac aaaccatagt ttacagcaat caatagtttt aaacgtgctt tttgactatt
     3961 aatactgtta cagaaaataa tccccatttt ttctagttct tttcccccgc cttcataatc
     4021 ataaacaggc tctgcgattc cattgaaaca acgagaaaca agtactacag ggattttttg
     4081 gacaagaagc ttttccaaag cagctaaggc ttgtggaggg agattccccg cacctaaagc
     4141 ttcgattacc aaaccgtcta ctttcgtatg ggcgatggct tccaataagt ccccttgcat
     4201 cccagcatat gctttaacga tcgggatagt cccatctaca gcggaaatgt ccagtcgctt
     4261 tgtttctaaa agttcctgca agaataaaat tcgattctta gttactaagc cgatgggacc
     4321 caatgtaggt gttcggaaag tagcaacatt tgtggtatgg gtctttgtca cgtagcgtgc
     4381 agaatggatc tcatcgttca tgaccaccaa aaccccctta tctagcgctt cttcacaact
     4441 tgcgacacga atcgcacttt caaaattata aaggccgtca cttccaagtt cattacttga
     4501 acgcattgcc ccagtcaaaa caattggtaa tttgttgcca atcgttgtat caagaaaaaa
     4561 agcagtttct tctaatgtat ctgtaccgtg tgtgatcacc actccggata tacctgatgc
     4621 ataagcggac aagatacgtt cttttaatac caacatgtga gaaggcatga tgtgtggtga
     4681 gggaagattg aagatatctt caactaataa ttctacatgc ttggggattg tgatttctgc
     4741 atctaataat gggtttgcca catcaggact aacaccgccc gtgagcatgt tttctttcat
     4801 ggcgatcgtt ccgccggtgt gtagtacaag tatttttttc attttacaga cttccttacg
     4861 aataattttg catagccgtt aaatcttatt gtatactata taaaagagtt ttgctatcag
     4921 tgaagaataa cgaatagcct aacttatcat aaaaaagctg accttattaa aatggaaaag
     4981 actcaacctt gacctcctaa tacaatggag tatctggttg agtttctttt acaagaaagc
     5041 agccaaggaa atcaatcacg aaggtggccg gaaatgatca gaatcggatt aacatcgttt
     5101 aaagaacatg acaaattaac tggtaaaaag aataacactc tttatgaata tgcagggttt
     5161 ctacccttag tggaattaga tactgcctat tatggaattc cccgaaagtc ctcagttgag
     5221 aattggataa gtgaagtacc tgaatcattt cgctttgtag tcaaagtcta tcaaggaatc
     5281 agcggtcaag gagactggaa gccttattat cagaccgaag aagagatgat tgcgcatttt
     5341 ttagatgtta tgtcgccaat ggtagatgct ggaaagatgt tttgcttttt agtccaattc
     5401 ccctcatcat tcaaatgtac gaaagaaaat gtccagtact tgcgcaaact tagaaagtgg
     5461 tttgagggtt atcctgtttc tatagaattg cgggattatt cttggtacag cgatgaattt
     5521 cttgaacgta caagaaactt tatgtctaga gccgcttttt ctttagccat catcgatgag
     5581 ccacagttgc tgaatacaac gattcctttt gatccatttg tcaccaatcc gaactttgct
     5641 ctttttcgat tccatggtag gaatgcgcaa ggatggcaag caaatgataa agagtggcgt
     5701 aaaaaaagaa cgttatatcg ttattcagaa aaagaactgc aagaactagc acatgaagtc
     5761 aaagtagttg attctaagac aaaagaggta ggtgtgattt ttaataataa ttctggtggt
     5821 gatgcggctg aaaatgcatt agcactgcaa aaaatattaa atatcaagcc agatggactc
     5881 aatccaaccc aaatggatct gttttaaggg cttattttat tcaagaaagg ggcgaagtct
     5941 atgatcaaag ctattttttt tgatattgac ggaacattgg tcaccaatcg ttcaaaagcg
     6001 ttggaatcaa caaaacaagc aattgaatat gctcggaaaa acggcatttt atgcggtgta
     6061 gcaacggggc gcagcccagt aaagatcaaa gaaatcattg atgaattgga attagatatg
     6121 tatgtcgtgt ataatggtca actggttttt actgcagatc gcacgattat cgaccaccct
     6181 tttgaacaaa aagtattgga acatattgtt gaatttgcag atgagaacca tcgacaaatc
     6241 gtttttggtg cgagaaatcg gttagatgga tcaacgacga tgctattagg acaatcgatt
     6301 tttatcaagc gactagtcag ctttttaccg cgtaaattcc cggtacgtct gatgaaaaaa
     6361 atcctccagg tttttagccc tcaccgacaa aaggatcgtt atgaaacgtt agagatatta
     6421 aaagaaccaa tttatcaatg tatattgctc agtccggagt cagaacagaa aaagttagaa
     6481 catcgctttc ccgagtgtac gtttcagcgt tcaaatacgt atactgtaga tatcatccca
     6541 aaaggtggtt caaaattact aggcatccaa gctttcgcaa atgctgtcga aattgaaatg
     6601 gaagaaatta tggcattcgg cgatcattac aatgatatcg agatgctgaa aggtgtagga
     6661 attggagtgg ccatggggaa tgctcagatc gaagtaaaac atgctgcaga ctttgtgact
     6721 caatcaaatg aagaagatgg gatatacgaa ggactgaaac acttcggtct gatttattag
     6781 tcaatgaaag aaggaaaaaa tcagtatgga agcagagaca ccttttcaaa aagtagaaac
     6841 attccacaaa acttttgatc caagaagacc agagataccc acggctttta cagaaaatga
     6901 agctcataca cgtgctgcgt tcaaaataga agaacttgtt gaatttgttg cagctacttc
     6961 acctaataaa gaacaactgc atgaagctgt tatgctgctg cacgaagcat tagataaagc
     7021 agaagaaaaa atacttggta aaacacagtg gggaagtgat ccgctggttg aacaagtaga
     7081 tgcactgaca gatatccttt actttactta tggttctttt tcattactag gaatagtgcc
     7141 tgatcgaata ttcgaaatcg tccatcaggc aaatatgggg aaattatttc cagatggaaa
     7201 acctcattat gaccctgtaa caaataaagt gttgaaacct gaaaactggc agatggaata
     7261 tgctccagaa cctaaaatcg cggcagaaat ccaacgtcag attcaagaag cgattgctaa
     7321 gaagaaatcc gctgatatag gcatgtgcac aaatgaaaaa gagtgatcag agtttcacta
     7381 tttttgttat ctttatacga tggaaaaaca aaagattttc gtttaagtta gtgaatgcat
     7441 gaaaatagta tgctataatc tagaatagat gtcttttgca cgagagacgt attatttgga
     7501 tttatgccaa gcagaacgcc atctgttgca taaaagatgc atactgaaaa cggaggaacc
     7561 ataaatggga aataatgtta tcatcggaat cgtcattgca atcattatag tcgccattgt
     7621 cgtctttttg gttagttact tgatgaagaa aaaaaaccaa gatcgattgg atgccttaga
     7681 aaaacgcaag gaagaattat ttgacttgcc agtgattgaa gaagtcgatg atgttaaaaa
     7741 aatgcatctg gttgggcaaa gccaaaatac ctttcgtgaa tggagtcaaa aatggaatga
     7801 catatctact tcctcgttcg ctgaactaga aagtcaaatt ttcgaagtgg aaaacctcaa
     7861 tgaaagtttt cggttcttaa aaggaaaaaa agcagttgtt gaagcagaag ctacaatgga
     7921 ccaaatggag aaacaagtga cggaaatccg tgaaggtcta aaggaattgc gggaaacgga
     7981 agaacgcaac tcactagctg ttcagcaggc acttgacgtc tatgaagaac tgaaagaaaa
     8041 agtcagagaa gacgagcatg aatacggacc agctcttttt gaattgcaaa aacagatcaa
     8101 agtgatcgaa aaagaattta cgcagtttgt gactttaaac acttctggag accctgttga
     8161 agcccgcgag gttttggata aagcagaaaa acatacgtat gaagtggaag ctctgatgaa
     8221 aaaaattcca ccgctgtatg aggagctcca taccactttc cctgaacaac ttgaagaaat
     8281 cagtgatact tacgagaaat tgatggaaga acaatatgta ttcccagaag aagatcttgc
     8341 tgaagatatc gcaaaagtaa gtcgaagaat tgaaaattca ttagccaatc ttgaaaaaac
     8401 agaagttgaa acggtcgaat ttgaaaaccg tgaaacagct gatttgattg attcgttata
     8461 tgatatctta gaacgggaaa tggaagctca gagatatgta aaaactaacc aatcaacgat
     8521 tgcagagtat atcaaacata caacaaagaa taaccgtcaa ttattgattg agctggatca
     8581 cacatctcaa agctatacat tgaaccacaa tgaaattggc cgtgtacgag gtttccaaac
     8641 cgaagtggaa gaaatggaac gtcaaaatga acagatgatt ccacagatcc gtcaacacga
     8701 aattccttat tcagaagtgc gcacgttcta taaaacagtc ttcaaagtct tagaagatat
     8761 cgaaaagcaa caagtagaga tcgacgactc attacatgaa ttgagaaaag gcgaaaaaga
     8821 agcgcaagaa aagatcgata cattcgaatt caaattgcgc agtttgaaac gttttgtaga
     8881 aaaacaacgt ttgcctggct tgccgaatga ttacctagaa ttcttttttg ttgctactga
     8941 ccgtattgaa gaactaagtg ttgtcttgaa taaaattcga gtaaacatgg aagaggtcaa
     9001 tcgtttagtt gctctatgtg aagaagatct agaattatta gataagaaga cacatgattt
     9061 agtagatgca gcagcattaa cggagcaaat gctccaatat gcaaatcgtt atcgtcatac
     9121 acacgaagaa gtgagagaag caattgataa aagctactat ttattcaata aagaatatca
     9181 ctatcaagaa gcattagacg aaatcggtac agcattagag cgagtagaac ctggtgcttt
     9241 caaacgcatc gaagattttt attttaatca tccagactta gtataaagca aaaaagttag
     9301 taaaccagca agagcaagac ggattattgt cttgttcttg tctttttttg tatatttgaa
     9361 aattgaaaca agtgaaaatt tcacctgctc ttgaaagcac cttggacaac cgttataata
     9421 gaaaagaatt tcaaaaaaga ggggtttctt atgatatatt ttgataacag tgcaacaacc
     9481 ccaatctttc cgcaaagctt agatgcatat gtaaaaacca gtcagcggat tattgggaat
     9541 ccatcaagct tgcatgattt aggcaatcaa gctaaccggt tgttgcagca agcaagaaag
     9601 cagatcgctg atttgatcca tgtagaacca gaagaaatct attttacttc aggcggaaca
     9661 gaaggagata actgggtatt aaaaggaacg atgatcgaaa aaagagaata tgggaatcat
     9721 atgatcattt caggagtcga acatccggcc gtcagcgaga cagcagaaca attaaaagaa
     9781 ttaggaatcg aagtaagtat cgccccagtg gataaacgcg gatttgtacg tgtcgacgaa
     9841 ttgaaagaat taataagaaa agagacagta ttagtctctg ttatggctgt gaataatgaa
     9901 gtaggatcga ttcagcccat ccaggaaatc agtgatcttt tggatgattt tcctaaaatt
     9961 catttccatg tagatgctgt tcaggcgatt ggaaaagtac cattctcgca atggttgaca
    10021 gaaagagttg attttgcaac attttcagct cataaattcc atggcccacg gggaactggt
    10081 ttcatttatt ggaaaaaggg tcgtcgttta gcaccattac tgacaggtgg agggcaagaa
    10141 agaaatcagc gaagtgggac agaaaatgtt ccaggtattg tggctatggt taaagcttta
    10201 cgtttatcat tagagataaa ggaacaaaaa cctgagcaga cacaacgctt aaaagtagct
    10261 ctaatcaatg cgttgaagac gtatgagaaa gtgacgattt tttcagaagg aactgactat
    10321 gcaccgcata ttttatgttt tgctctaaaa ggaattcgcg gcgaagttct cgtccatgcc
    10381 ttagaagaaa aacaaatatt tgtttctact actagtgcct gctcctcacg taaacatatg
    10441 gcaagcagta cattgcacgc aatgcatgta ccaaatgatc tggctacatc tgctgttcga
    10501 atcagcttag atgaaaacaa ttcgcttgca gaagtcgagc aatttatgat tattttcaag
    10561 cagctttata agaaattttc tgcaatcaat taaaagagga gtgttcttgt gaaatacagt
    10621 gaaattatgg tccgttatgg ggaactttcg acaaaaggaa agaatcgaaa aagctttatc
    10681 atgcaactag cacaaaatgt cagacaagct ttgaaagact ttccagaaat caaaatccat
    10741 gctgatcggg accggatgca tcttttattg aatggcgcag acgggcagct agttattcca
    10801 aaactagcaa aaatatttgg gatccaaaat ttctctccaa gtattcgtgt agaaaaagat
    10861 gtcgaagttt tgaaacaagc tgttcaagac atcatgaaag aaatttatac tgggaaagaa
    10921 acatttaaaa ttatcgctaa aagaagcgat caccaatttg aattgacaag taatgaattg
    10981 aatcaaacat taggtaatgc tgtttttgat attttcccgc atatccaagt tcaaatgcgt
    11041 caaccagata ttccattacg tgtagagatt cggcgtgatg gcgcttatct atcgtatgaa
    11101 acgatcaagg gagctggagg attgcccgta ggaacaagcg gaaaaggaat gcttatgctt
    11161 tctgggggga ttgattctcc tgtagcaggg tactttgcca tgaagcgggg agtggaaatt
    11221 gaagctgttc attttgctag tccgccgtat acaagtgaac aagcactgca aaaagcaaaa
    11281 gatctaacag ccaaactagc accttatgtg ggaacgatcc attttatcga agtgccattt
    11341 accgagattc aagaagaaat caaaaagtca gtgccacaag gatattggat gacaattact
    11401 cgtcgaatga tgttgcgttt gaccgatgaa attcgccgta ttcgtcatgg tttagtcatt
    11461 ttgaatggtg aatcattagg tcaagtggct tcacaaactt tgcaaagtat ggtggctatc
    11521 aatgaagtaa cgaatacgcc aatcattcgg cctgtagcga caatggataa acttgaaatc
    11581 attgaagtag cagaacagat cgatacattt aatctttcta tccagccttt tgaagattgc
    11641 tgtacgattt ttgctcctcc acagccaaaa acacgccctc aactggaaaa ggtccatcaa
    11701 tatgaagagc gtttagcaat agatgaaatg atccaacgag cactagctgg cttgaagatg
    11761 gaagagatac aagcagaatc gtctaatgaa aaaactgaag aattttcaga attgctataa
    11821 gaggttatga aaatagtgtt ttgaccagag tattagagaa acaaaacgaa aaaatagctc
    11881 catgtatttt accgtttttg aactaatgtc gaagattagc tgattgaata ccatttatca
    11941 agaagttatg gaaaagccgg ttctttttta ctaactgaaa aataaaattt tatttagaga
    12001 tcaagctaca gttgataaat gatataaaaa agagaatttc ccatgaaata gcgaggttgt
    12061 gacagaagtg ggcagcttca agaaataaga aggaatttac gaaaattgct tttcaaattt
    12121 ttgtgaaatt tcggcttatt tttcgaagaa gttacttctg gaacaccgtg tatcagaggt
    12181 tgcgaaaaag ctgccctgca tcaagtaata agaacagcca aacaaaaata gttcctcata
    12241 ttttacgttt ggttgagctt attaccgcag atgcaagctt ttgaacaccg ttttatttgt
    12301 ctcacaccct tttccttcct acttgtaacc ttgctcccgt gttagaaaca tgttacacta
    12361 aatatgtgaa attattaaca aggagcgtcg ttatgcgaga aaaaacagat acactgccaa
    12421 aagcaactgc gaaaagatta cctttatatt tacgctactt gaagatgctg gatgactcag
    12481 gtataagtag aatcaaatca aatgagttca gcgaaatcac ccaaattcct tctgcaacga
    12541 tccgcagaga cttctcgcaa ttaggcgagc taggcagaag cggatatggc tatgatgtcc
    12601 cttttcttat cgatgtattc aataatatcc ttaatactaa agaagaaaaa cgaatcgcat
    12661 tagtaggata tggaaatctt ggcaaagcat tgaaacacaa caattttaga agaaatgaaa
    12721 atctaaatat cgtgtgcgtc tttgataatg atccagcact gatcaatcga gtaattgatg
    12781 gagaaatgat ctatccgatt gatcgtttcg cggagatagc caaagcaaaa aatgtgacag
    12841 tagcaatctc tacggtacca agtaaatatt cacaatctgc tattgatgaa attgtcaaag
    12901 gaaatgtaac cgctatactt aattttgcgc ctgacagagt aactgtccca gcttatgtca
    12961 atgttcaata cattgatttg acaaccgagt tgcagacgct tatttacttt gatgagaatt
    13021 actcagaagt tttttcttag gaaaatttga gtaggcttat tgatcttgcg agaactcgtc
    13081 gtaattcttt caaaaatttt ttagtactaa atgaagtggt tttttcacga ccacttgttg
    13141 actaaaaaaa aaaaccattg tagaatgaag cataacgctg aacaagagga gtaactggca
    13201 cattcattct tagagagaga actatctgct gaaaggttct caatgacaac cagtgaaggt
    13261 agcttgggag tttctgtctt gaatggagta gagatagacg agtcgtgatc gttaactcac
    13321 ttgagaggca ataatgattg attattgcaa tttaggtggt accgcgtaaa tcatacgtcc
    13381 tagaatacga aagtatttta ggacgttttt tagtttaaag gaggaaaaca gcatgacgga
    13441 agaaaataac ctatcaacaa aatataatcc gcaagaagtt gaagctggac gttatcagca
    13501 ttggttagaa caagatttgt ttaaaccgag cggagataaa aaagcaaaac cttattcaat
    13561 cgtaatcccg cccccaaatg ttactggaaa acttcattta gggcatgctt gggatacgac
    13621 cttgcaagat atgctgattc gtcaaaagag aatgcaagga tatgatactt tatggctgcc
    13681 tggtatggac catgcaggaa tcgcgacaca agcaaaagta gaagcgaagc ttagagagca
    13741 agggatttct cgttatgatc taggtcgtga aaagttcatc gatcaagtgt gggaatggaa
    13801 agaagagtat gcgagccaca tccgcgaaca gtgggctaaa atgggcttat ctttagatta
    13861 cagtagggaa cgttttacat tagatgatgg attatctgaa gcagtacgta aagtttttgt
    13921 taccttatac gaaaaaggtt tgatttatcg tggtgaatat atcatcaact gggatcctca
    13981 agcacgaaca gctctatctg atatcgaagt tattcataaa gatatcgaag gcgcctttta
    14041 tcatatgagc tatgaactag cagatggatc aggcgtagta gaaattgcga caacccgtcc
    14101 agaaacgatg ttgggagata cggcgattgc tgttcatcca gaagacgagc gttatcaagc
    14161 gctcatcggc aaaaaagtga ttctaccttt agtcaataaa caaatcccaa tcattgcaga
    14221 tgaatatgta gatatggaat tcgggacagg tgtagtaaaa atcactccag cacatgatcc
    14281 aaacgacttt gaggtaggta atcgtcatga tcttccacgc gtaaatgtta tggatgatga
    14341 tggtacgatg aatgaactag caggaaaata tgctggaatg gaacgttttg ctgctcgtaa
    14401 agcaatcgtg caagatctga aagaaatcgg tcgattgatt aaaatcgata cgatgatcca
    14461 cagtgtgggt cattccgagc ggacaggtgt cgttgttgaa cctcggctgt ctactcaatg
    14521 gtttgtaaaa atggcgccgt tagcgaaaaa agccattgat aaccaagaaa cagatcaagc
    14581 agtggaattc ttccctccaa gattcaatca aacgttttta cgttggatgg aaaatgtcca
    14641 tgattgggta atctcacgac aattatggtg gggacatcaa attcctgcat ggtatcacaa
    14701 agaaacaggt gaaatgtatg tagggatgga agcaccagcg gatagtgaaa actgggtaca
    14761 agatcctgat gttttagaca cttggtttag ttcagctttg tggcctttct caacaatggg
    14821 ctggccagat acggaaagcg aagattataa gcgatacttc cctactagca ctttagtaac
    14881 cggttatgac atcattttct tctgggtaag cagaatgatt ttccaaagct tggaatttac
    14941 tggcgaacgt ccatttgaga atgtcttgat tcatggattg attcgtgacg aacaagggcg
    15001 gaagatgagt aaatcattag gtaatggaat cgatccgatg gaagtaatcg aaaagtatgg
    15061 agcagatgct ctccgctggt tcttatcgaa tggttctgca ccaggacaag atgtccgctt
    15121 cagctatgaa aaaatggatg cttcttggaa ctttatcaac aaaatctgga atgcttcacg
    15181 tttcgttatc atgaatatcg aaggaatgac cgtagaagag atcgatttat ctggagaaaa
    15241 atcagtggct gaccggtgga tccttactcg tttgaatgaa acgatcgaac gagtaactga
    15301 gctatttgat cgttttgaat ttggtgaagc aggtcgacaa ctgtataact ttatctggga
    15361 tgatttttgt gactggtaca tcgaaatgag taaagaagta ctttatggag atgatgctgt
    15421 ttcaaaacaa accacccgta gcatcttagt ccatacactt gatcagatcc tacgtttatt
    15481 gcatccaatc atgccattcg ttacagaaga aatatgggaa catattcctc atcagggaaa
    15541 ttctctagta gtggcagaat atcctgttgt acgtccagag tttgatgatg aaacagcttc
    15601 taaagggatg gaagtattga aagaattgat tcgagccgtt cgaaatattc gttcagaagt
    15661 gaatacacca ttgtctaaac caattacgtt gctaattaaa acaaacgatg ctaaaatcga
    15721 tcaattttta gttgaaaata caagctatat cgaacgcttc tgtaatcccg aagagttgac
    15781 gatatcaagt gagatcactg cgccagatct agcgatgtca gctgttctga caggtgcaga
    15841 aatcttcttg ccgttagcag gattgatcaa tattgaagaa gaaatcaaac gattagaaaa
    15901 agaactggaa aagtggacaa gcgaagtaaa acgtgtccaa ggaaaacttt ctaatgaacg
    15961 ttttgtttct aacgcgccgg aagaagtagt agaggcagag cgtgcaaaag aaaaagatta
    16021 tttagagaag caaacggctg tcaaagaacg aatcgaaagt ttgcgtacta ttcaataaga
    16081 atagaaaaag agagtgtgaa atcagctagc tattactaaa agctagctga ttgaacatcg
    16141 ttacaggaaa gagtttgagc ctcactctta gcttcgattc ataaggagat gtgacataaa
    16201 ttttgtcgca tctctttttt agtctactct tattatgtag gagaaggagg gaggtggatc
    16261 atgtatcaag agtcaataaa gtagttgata aaatcgttcc tctctctgtt tcttttctct
    16321 ttgtcatact tttatttttc ttatgtgttc tatttgttat aataaaagag aaatggggga
    16381 attgttgatg acgattgaag aagcaataga atggatccat agtcgattac catttggttc
    16441 acgtcctggc cttgaccgag tcgaagcttt attagaagtg gtagggcatc cagagagaaa
    16501 agtaaaaacg atccatatcg ctggaacaaa cggaaaaggt tctactgtag cttatttgag
    16561 aagcctatta gaagaggctg ggctgacagt aggtacattt acttcacctt atatcgaaac
    16621 gttcaatgaa cggatcgcaa tcaatggcaa aagcattcca gacaatgatt tgatccagct
    16681 tgtaaaaaaa tatcagccac tgatcaaaca acttgatcaa tttgaagcag tagctggtat
    16741 tactgaattc gagacattaa cagcaatggc atttgattat tttttgatga agaaagtgga
    16801 cgtagcgatt atcgaagttg gcttaggcgg cttattggat tctaccaatg tagctgtacc
    16861 aatgctgacg ggaataacaa cgattggact tgatcatacc gaaatacttg gagatacaat
    16921 tgaagaaatc gctgcacaaa aagctggaat catcaaacaa ggcgttccag tagtaacagg
    16981 aaatattgcc ccagaagctt ttactgtcat ccaacaaaca gctaaagcaa aaaatgcacc
    17041 gctttatgta tacggggaaa attataaaat aaactatctg catcctgatg agatgtgggg
    17101 agaagtattt gaatttttta atgaaatagg aaagatccct caattaacca ctccgctttt
    17161 agggcaacat caagctgaaa atgcaggagt agccatcgaa ttgtattttg tttactgtca
    17221 attggaaaag ctgcctttcc gagaaaaaga tgttttgaag ggattgaaga aatctcgatg
    17281 gccaggaaga atggagcgtt tgagtaacga gccattagtt gttttagatg gtgcccataa
    17341 tacgcatgca atgaaccggc ttgttgagaa tttgaaaaaa gaattcaaag gatatcaaat
    17401 caacattttg ttttccgcct tacgaactaa aaatgttaga gaaatgctag aacgattgca
    17461 gcaagtacca aatgtccata tttatttgac gacctttgaa catccaaaag ccatcgaatt
    17521 aagtgaagta tcagatttag caagtgagag agtatcaatc gtttcattgt ggcaattcgg
    17581 attagcagat atattggaaa agatgacaaa tgaagacgta ttagttgtga ccggttcact
    17641 ttatttcatt tcagaagttc gccatttatt aatacaatta ggaggagatc atgaagtata
    17701 atgcagtgat ttttgacatg gatggtttgc tgttcgacac agagttagtg tattacgaag
    17761 ccacacaagt agtagcagac aagatgggtt ttccttatga taaagacctg tatttaaaat
    17821 tcttaggtgt ttcagatgaa gaagtatggg cgaattatca tatgattttc tcggaatttg
    17881 gcaaaacaag tgtacaaaga ttcatagatg attcatacca ggaaacgctt gaacgttttt
    17941 cgcaaggagc ggtacaaatg aagcccggtg tgttggaact cctctctttt ttagaagaac
    18001 atcagattcc caaagttgtc gcttccagta accaacgagc agtcattgaa cttttattga
    18061 agaagaacaa tctgtttgaa cgatttgaga cgattgtttc tgcagaaaat gttcaacgag
    18121 caaaaccaga cccagaaatt ttcttgttag ctaatcagaa gctaggtaca gcgaagaaag
    18181 aaacattgat tttagaagat tcacaaaatg gtattttagc agcttctgcg gctgagatcc
    18241 cggtagtcat ggtaccagat ctgcttccac catcccaaga attgatcaaa aaaacagctg
    18301 gggttgtttc ttctttacat gaaatccctg cattgttaaa ataaaaatcc atctcttttc
    18361 gtatcttatt ttagataaga tagaaaggag atgttttttt gggaaaacga ttgatcaaag
    18421 aagtacctac tagctcttta ccaagggaac gtatggaaat ttatggcgcg gaagctttat
    18481 cggatcagga gcttttggca atcttactgc gtaccggtca acacccttat agtgtgatgt
    18541 cgattgctgg aaatctgtta aaaacgtttg gaggactggc ctctttacga caggctactt
    18601 tgcatgagtt agaagagatt cgagggatag gacgcgtgaa agcaatcgaa atcaaagcct
    18661 tgatcgaatt aggtcgacga attcagacgg atcatgaaag agcggctcca ccggttcgta
    18721 ccagctacga attggcccag caattgattc tggaaatgaa agatcacaaa caagaacatt
    18781 tggtttgtat ttatttggac acgaagaatc aagtgatctt aaaaaaaaca gtgttcatcg
    18841 gttcgctgaa tcagtcagtc gctcatccta gagaaatttt ccattatgct gtacgttatt
    18901 gtgcagcaag gatcgtttta gcgcataatc atccttctgg taatgtttta accatagtat
    18961 aataaataat agtagttaaa gtagtttaag aggagatatt aaagggatat tttactttaa
    19021 ataaaaatta acaaggttta taatttgtag tagttaaagt atttaaagag gtgggaacat
    19081 gtgaaggttc aaaggataga agtggagaat aagccgtatc cattgtattt attactagat
    19141 aaagaatacc agctaataga accagtaatg aaatttatta aatacttaga taatactggt
    19201 aagtctccta ataccattaa agcatactgc tatcatttaa agttgctgta cgagttcatg
    19261 gaacagagag gtgttattct taatgatatt aactttgagt tgttagcaga cttcgtaggt
    19321 tggttgagat atccttcagc atcaaatgta attgatcttc agtcaaaaaa agccataaga
    19381 gaagaaacga cagtgaatac aattttaaat gtagttatga gttttcttga ttatttaagt
    19441 agattaggag aatttaaatc aattgatgta tttaaacaag caaagggaag aaatttcaaa
    19501 ggatttttac atcatgttaa taagggtaga taccaaaaga atgtcttaaa gttaagggtt
    19561 aaaaagaaac agataagaac attgagatca gaggaagtta agcaaattat tgatgcttgt
    19621 catacgaaaa gagataaatt aattttaatg cttatgtatg agggtggttt aagaatcggt
    19681 gaagtgttat cgcttaggct tgaagatatt gtcacttggg acaatcaaat ccatttaaca
    19741 cctagagatg ttaatgttaa tgaagcttat attaaattaa ggaaggaaag aacaatacat
    19801 gtgagtaaag aacttatgtc actttataca gattacttga tatatgagta tagtgaggaa
    19861 ttggagcatg attatgtttt tatttcctta aaagaaggct attttgggaa accactaaag
    19921 taccaaagtg ttcttgatct agttagaaga atagttaaaa ggactggaat agaatttaca
    19981 tcacatatgc ttcgccacac tcacgcaacg cagctaatta gggaaggatg ggatgttgcg
    20041 ttcgttcaaa agagattagg tcacgcacat gttcagacaa cgttaaatac ctatgttcat
    20101 ctttcagatc aggatatgaa aaatgagttt aataaatacc tcgagagaaa ggagcataag
    20161 aaatgaatgc ttctagtaaa aggaaaatta ttagtcagag tgagattagc aaaaaaatag
    20221 ctgtaatgaa tgaagaaatg caggggtttt gggctaataa tagttgggat ataagaaaat
    20281 gtccacatcc ttctgccata gaattaagta agaatcctgc tttaaggaat cgttgggttc
    20341 gttttgaacg tgttaaaaat ctgtggttaa gaacagaatt gaaatatttt tatttttacc
    20401 atttaaacaa tggaatatgg aatgcaaaaa ctgtctggat tagaaaagga acagtaatta
    20461 ataaaatgtt agatttctta gatttaaagt atcctagcat tacttcaatt actgaagttc
    20521 ctattgaaaa agcaatgacg gagtatagaa cttatttgac aaaacggggt gttagaatta
    20581 ccactactaa ttataagatt actgctaatc aagaaaaaac acctgtaaaa gctaattcct
    20641 actatgttac taatctaaaa caatttatgg agttttatga gaacttttat tttgatggag
    20701 aggagtggga taaagacgtt tgggatagac gtaacttacc tttgccagat gataaggtta
    20761 acccaacaca atatgaatat acaattaact ttaaagggtt tcggaataca tattttaaac
    20821 aacttgtaaa aagatattgt aagttgagat tgaacatgga tagcttttcc tatgtaagtg
    20881 atattgccca aagacttaaa gagttcttta attttctgga catgaaattt aaacaagttc
    20941 agagagtaca ccaattaacg agagtggaaa ttgaagcata tttaagtgaa ctaaacatga
    21001 tgggaataaa acctagtaca ataactggga ggatctctat attggaagga ctatttagta
    21061 cccttcttag gctagaatgg gatgatgttc cttccaaaat attaatttat tctgaggact
    21121 atccgaaaat accaagagca aaaccacgct ttatagatga attcgtccta gagcaattga
    21181 acagtcatct tgataaatta cccgaatata tagctacgat gactatgatt gttcaagaat
    21241 gtggaatgag gataagtgaa ttgtgcacct tgaaaaaagg ctgtctatta gaggacaaag
    21301 atggagattt ctttttaaag tattatcaat ggaaaatgaa aaaggagcat atagttccaa
    21361 tatctaaaga ggtagcttta cttattaaag ttcgggaaga taaagtttca gaggaatttc
    21421 cagatagtga atacctcttt ccaagaaaag atggatcgcc attaaaacaa gaaacattta
    21481 gaggtgagtt aaataaatta gcttatgagc aaaatatagt ggataaatca ggtgagattt
    21541 atagattcca tgcccatgcc tttcgccata cagtaggaac aagaatgatt aacaacggga
    21601 tgccccagca tattgtgcag aaatttttgg ggcatgaaag cccagaaatg acaagcagat
    21661 acgctcatat ctttgatgaa actctaaaaa atgaatttac taaatttcag gaaaaactgg
    21721 ttaccaataa tggagatgtg cttgatctag atgaagataa tgaagtcgat gatgtagagc
    21781 ttcaatggtt caagaaaaat ataaatgcac aagtgcttcc aaatggttat tgtagattgc
    21841 cagtagtagc aggtggttgt ccacatgcga atgcatgctt agattgcact cacttctgta
    21901 ccagtaagca attcttacca cagcacgaag aacagttaga gcgtacagaa gagttattag
    21961 ccatagctaa ggataaacaa tggcaaagac aagtagagac taatagccgt gttaaagagc
    22021 gtttagaaca aatcattgga agtttgacgg ggtaattatc aatggataaa caagttagaa
    22081 atacaacaga aattgtacgt ttggcgaagc agaaatcaaa aaagacaagg gaaaaagtag
    22141 acaaagcgat ttctaaattt tcgattgaag gtaaagttat taattttaat tcaatagcaa
    22201 aggaagctaa tgtttctaaa tcatggcttt ataaggaaca cgatattagg caaagaatcg
    22261 aatcccttcg tgagcgtcaa ataacagcaa atgtagtctc aaaacccaag aaaagttctc
    22321 gttcggagga aatccttatt aaaaccttaa aaagaagagt aatggaatta gaaaaagaaa
    22381 ataaaaaatt acagaaccaa attcaaaaat tatatggaga tctgtataat aaagaataat
    22441 tattaatctg tagacaaatt gtgaaaggat gtacttaaac gctaacggtc agctttattg
    22501 aacagtcatt taggtatatg tccaatctag ggtaagtaaa ttgagtatca atataaactt
    22561 tatatgaaca taatcagtta cgaggtgaaa tcatgagcaa tttgattaac ggaaaaatac
    22621 caaatcaagc gattcaaaca ttaaaaatcg taaaagattt atttggaagt tcaatagttg
    22681 gagtatatct atttggttca gcagtaaatg gtggtttacg cattaacagc gatgtagatg
    22741 ttctagtcgt cgtgaatcat agtttacctc aattaactcg aaaaaaacta acagaaagac
    22801 taatgactat atcaggaaag attggaaata cggattctgt tagaccactt gaagttacgg
    22861 ttataaatag gagtgaagtt gtcccttggc aatatcctcc aaaaagagaa tttatatacg
    22921 gtgagtggct caggggtgaa tttgagaatg gacaaattca ggaaccaagc tatgatcctg
    22981 atttggctat tgttttagca caagcaagaa agaatagtat ttctctattt ggtcctgatt
    23041 cttcaagtat acttgtctcc gtacctttga cagatattcg aagagcaatt aaggattctt
    23101 tgccagaact aattgagggg ataaaaggtg atgagcgtaa tgtaatttta accctagctc
    23161 gaatgtggca aacagtgact actggtgaaa ttacctcgaa agatgtcgct gcagaatggg
    23221 ctatacctct tttacctaaa gagcatgtaa ctttactgga tatagctaga aaaggctatc
    23281 ggggagagtg tgatgataag tgggaaggac tatattcaaa ggtgaaagca ctcgttaagt
    23341 atatgaaaaa ttctatagaa acttctctca attaggctaa ttttattgca ataacaggtg
    23401 cttactttta aaactactga tttattgata aatattgaac aatttttggg aagaataaag
    23461 cgtcctcttg tgaaattaga gaacgcttta ttactttaat ttagtgaaac aatttgtaac
    23521 tattgaaaat agaaagaaat tgttccttcg atagtttatt aatattagtg acatttgcat
    23581 gcttcaaagc ctgtcggaat tggtttttag tgaaaagaac acgatattca cggtttaccc
    23641 acttataaac aaaagatcga tactttttgt agtccttctt tgaaatcaat ggttgatgtc
    23701 gttcaagaac aatcaataca gagtctacac ttggcttagg atgaaaatat agtggtggta
    23761 cttttttgag catttttata tccatctcca ccattaatag taaacccaaa gctcgttgca
    23821 gattttgcaa tcttttcgca aatcccttct caacgataag atagctatat ttagcctgac
    23881 tttcaaaggt aattcttttg acaatatccg tactgatgtt ataaggaata ttaccatata
    23941 tcttatagtt tatatgtttt gggaaggaaa attttagaat atccgtttga atcactttta
    24001 tattctcaga ggggtttacc gcttctttag tcacttgaca taagcctcca tcaatttcta
    24061 tagcagtaac tgatcgactc attttgacta gctctttggt aaaatgtcct tttcctgatc
    24121 cgatttctat tacgttgtct tgtttactga tattcgtgtg attcaatatt tcttttacat
    24181 gctttttaga agtaataaaa ttttgcgtgt ctttagggtt tttctggttc attataacct
    24241 tctccttact ggttataatg aactgacatt aagcgttcat tataaccaat taattttgat
    24301 ttggttgata acgaacttta ttaataacaa atatagaaaa agtacccata cttttatctc
    24361 ctttccattt ttttcattat gaatgagata aagtaatttc tactactgcg atactggtgc
    24421 acataattga aacctcctta tgtaactgaa tatgattgta tcatcttttc attttagaat
    24481 aaacctttat atgtaatttt gcacttgaca cttcagatgg acgttttctt caaaataata
    24541 tgttttgaaa cttgataaga gaagccactg tctttactaa aaagtaaagg ctgcgataaa
    24601 ggacagtgac tttaataaag ttgcactgtt gtattgtctt tggataaaat tgtctaaagg
    24661 gaagttttat ccccaacgaa aaaacatcta aagtggtaag taatgaaaga atgtcgataa
    24721 agtaggaatg ggggatcttt ttgaatacaa tagaatcaca gtttgataag gttgcagaag
    24781 attacgattt cgtgaatgag cttttgaatg attattcatt ctttgtgtct aatatgtctc
    24841 caaagaaagg cagagcatta gatatcggat gtggctcggg tttgttagtg gagaaattag
    24901 caagttatta tgatgaagtg gtagggattg atatttctaa tcaaatgctc gatcttgcca
    24961 aatctaaacg tcaactaaca aatacggtct atctgaatat gaatgcagaa caacttaatt
    25021 ttaatgagaa gtttgatttt attgtaagcc gaacaacctt tcatcatttg gatgatatag
    25081 ccagcgtaat acagcaaatg aaggaactgt tgaatgagga aggaagaata gttattcttg
    25141 ataatgtatc tgaagtcgag acaccgccta cctatgttta taaattaggg gcaatccaag
    25201 agtttttacc gcattgtttc aaatttggaa taaaaaatgc gattagaatt tataatcaca
    25261 acacatctaa atcatggctt gagcatttag cttcagataa gtacctatct gaacaaaatt
    25321 attacgattt atatgaaaag ttgttgcctg gatgccaatt ccataaaatg ggttgggcaa
    25381 tgggggttgt ctggacaaaa taacttaaag ctgtggcttt gaaaagttga atttgtgatt
    25441 gttttcagag ctatcgggtg ctttagtata aataacaatg aataaaaaat cagcctgtat
    25501 cttatgttac tattaagtaa tttaagatac aggttttact atttttgaaa taagtacctt
    25561 ccacttcaaa tacatgtatt caaagtgaaa gtattgattt aaatgattct tttgtaattt
    25621 actcttgata atatgaaatt ccatcgcagc aggatatgaa ttttaccaaa aggatacaaa
    25681 agtgtggaga aatgatgggg atcactgtac tggaccactt gatcatcgga cgcaaacgat
    25741 attttagttt gagagaagaa ggaatgatgg aagagaagta agttgctttc ttgcaaaaaa
    25801 aatgaagcaa gtgtacaatt atattgtaat tttgtttggg aatagaagtt gaaacatctg
    25861 ttgcacctct atcgtacacg agcaatgtta caatttaaat tagtgctttt ggcacgagga
    25921 ggatattatt atggaaaacg gaacagtaaa atggttcaac gctgaaaaag gatttggatt
    25981 tatctcacgt gaagatggaa gcgacgtatt cgttcacttc tcagctatcc aaggtgacgg
    26041 attcaaaaca ttagaagaag gtcaagcagt gactttcgac gttgaagaat cagaccgcgg
    26101 cccacaagct gcaaacgttg taaaagctta attttatctg taaatcaaat tgatggtaga
    26161 catcaatttt tacgagagaa caaagcgagt aacaaaaata cctgccacaa gagacaattt
    26221 ct
//
LOCUS       DARBDV010000032        25979 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000032 DARBDV010000000
VERSION     DARBDV010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.032"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..258)
                     /locus_tag="SCM56_001261"
     CDS             complement(<1..258)
                     /locus_tag="SCM56_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110073.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAFL"
     gene            complement(274..924)
                     /locus_tag="SCM56_001262"
     CDS             complement(274..924)
                     /locus_tag="SCM56_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110074.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(935..2107)
                     /locus_tag="SCM56_001263"
     CDS             complement(935..2107)
                     /locus_tag="SCM56_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEG1110075.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(2126..2998)
                     /locus_tag="SCM56_001264"
     CDS             complement(2126..2998)
                     /locus_tag="SCM56_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG1110076.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(3020..3940)
                     /locus_tag="SCM56_001265"
     CDS             complement(3020..3940)
                     /locus_tag="SCM56_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110077.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(3940..4209)
                     /locus_tag="SCM56_001266"
     CDS             complement(3940..4209)
                     /locus_tag="SCM56_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110078.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(4206..4586)
                     /locus_tag="SCM56_001267"
     CDS             complement(4206..4586)
                     /locus_tag="SCM56_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HEG1110079.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
     gene            complement(5053..6516)
                     /locus_tag="SCM56_001268"
     CDS             complement(5053..6516)
                     /locus_tag="SCM56_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEG1110080.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(6580..9597)
                     /locus_tag="SCM56_001269"
     CDS             complement(6580..9597)
                     /locus_tag="SCM56_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG1110081.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(9748..10587)
                     /locus_tag="SCM56_001270"
     CDS             complement(9748..10587)
                     /locus_tag="SCM56_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG1110082.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            10732..10977
                     /locus_tag="SCM56_001271"
     CDS             10732..10977
                     /locus_tag="SCM56_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110083.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            11040..11462
                     /locus_tag="SCM56_001272"
     CDS             11040..11462
                     /locus_tag="SCM56_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110084.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(11556..11873)
                     /locus_tag="SCM56_001273"
     CDS             complement(11556..11873)
                     /locus_tag="SCM56_001273"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110085.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(11900..12106)
                     /locus_tag="SCM56_001274"
     CDS             complement(11900..12106)
                     /locus_tag="SCM56_001274"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110086.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(12087..12269)
                     /locus_tag="SCM56_001275"
     CDS             complement(12087..12269)
                     /locus_tag="SCM56_001275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110087.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(12283..12633)
                     /locus_tag="SCM56_001276"
     CDS             complement(12283..12633)
                     /locus_tag="SCM56_001276"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110088.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(12659..12961)
                     /locus_tag="SCM56_001277"
     CDS             complement(12659..12961)
                     /locus_tag="SCM56_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110089.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(12964..13197)
                     /locus_tag="SCM56_001278"
     CDS             complement(12964..13197)
                     /locus_tag="SCM56_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110090.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(14183..14505)
                     /locus_tag="SCM56_001279"
                     /pseudo
     CDS             complement(14183..14505)
                     /locus_tag="SCM56_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14628..15743)
                     /locus_tag="SCM56_001280"
     CDS             complement(14628..15743)
                     /locus_tag="SCM56_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEG1110091.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(15749..16147)
                     /locus_tag="SCM56_001281"
     CDS             complement(15749..16147)
                     /locus_tag="SCM56_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEG1110092.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
     gene            complement(17176..17562)
                     /locus_tag="SCM56_001282"
     CDS             complement(17176..17562)
                     /locus_tag="SCM56_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HEG1110093.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(17559..18080)
                     /locus_tag="SCM56_001283"
     CDS             complement(17559..18080)
                     /locus_tag="SCM56_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEG1110094.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            complement(18082..19116)
                     /gene="sppA"
                     /locus_tag="SCM56_001284"
     CDS             complement(18082..19116)
                     /gene="sppA"
                     /locus_tag="SCM56_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEG1110095.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(19149..20564)
                     /locus_tag="SCM56_001285"
     CDS             complement(19149..20564)
                     /locus_tag="SCM56_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEG1110096.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(20533..22686)
                     /locus_tag="SCM56_001286"
     CDS             complement(20533..22686)
                     /locus_tag="SCM56_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEG1110097.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            22944..23180
                     /locus_tag="SCM56_001287"
     CDS             22944..23180
                     /locus_tag="SCM56_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEG1110098.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            23199..23414
                     /locus_tag="SCM56_001288"
     CDS             23199..23414
                     /locus_tag="SCM56_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HEG1110099.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(24117..24869)
                     /locus_tag="SCM56_001289"
     CDS             complement(24117..24869)
                     /locus_tag="SCM56_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1110100.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(24884..>25979)
                     /locus_tag="SCM56_001290"
     CDS             complement(24884..>25979)
                     /locus_tag="SCM56_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEG1110101.1"
                     /translation="SYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFE
                     GIKGYGYVVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVS
                     YVAHQYQVFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYT
                     DSLEKDQEKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYR
                     YLKNIKNEYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAI
                     EASIESQDRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSS
                     IEEINKKNPNMFVNYQNDPYQFTTQVILLF"
BASE COUNT         7476 a         5115 c         3911 g         9477 t
ORIGIN      
        1 caaaaaggca agtgcttctt tgtaggcatt ttgactgtat ttttttgata aagcatgaca
       61 aatttcttga taaaatagtt gactgcctac cgaataccga ttattgtgta tccgccacaa
      121 aacgaacaca ctgatttgaa tcgacgggcg taaaaaagaa aaattctttc cataagaaaa
      181 agaacgtcct tcttgggttt cttttgcttg ttctgttggt acttcttgta cctctcttat
      241 tgattcttgt ctctccataa ccttctcctt ttcttattct tctaaaagct gttgattaaa
      301 gctacttccc aaattttgca taaacgtgat tttctttgac gctggatcaa aggttacgtc
      361 aaaaatattc atggcattat tccaggaact atcaccaatt ttattgcgat acgacaccac
      421 aacgagccct tggatagtcg ctttttgtgt gctttttaaa taaacagaac atttctctaa
      481 ttcagaagac accccgtttg aatcaccata ggaaacgcct tctttgaaat attgcttcat
      541 cagtttgtca gaaagatatg gtgcacaatt gttttttcgt tcttgaaaag aatcaggctc
      601 ataattaaac agaacctcga acgcttctgt cactgttttt tgaaaggttt gaatgtttcc
      661 tttttctgtt gaatcattgt ttttcaattc ttcatttgct ttttttaacg tcgtgacctc
      721 gctttctact tgttgaagtt gttctttgag tgacgttaca aggtcttcct gtcctttctt
      781 ctcttcttgg aacttgttta tctccaccgt cagtttctct tcctttgctg ttagctgtag
      841 cttttcatga ataagaaaaa atgtactgcc tacaccaaat aagaacaatc cgcctacaca
      901 aacataaata aatcgtttct tcatttgaca cctcttattt ctttatcggt cgagcaaaac
      961 aaatatcacc accattaagc aaatacccaa aggataaatt gacttgccct tcacctacac
     1021 cgttagtatt cattccccct tgttcgatta ctttcgtaga aagtccatgc caatcttccg
     1081 ctaaaacagc tgtgtgtcca tcatttccca caccagaacc taagttcaca atgataatat
     1141 cgccagcttt tgcctcgctc tcaggtattt cttgtaagta ttgcttactt cctcttgcgt
     1201 cgctcgtcat tgagccggta aaccacccaa cgttcgcagg cgttttatac cctgctttcg
     1261 ttaaaacaag ccacacaaag ctcgaacaat cggcatagcc atttttatct ggattttcga
     1321 cactaccaaa ttgtgcccgt tgcggttgtg aataatgaaa ataaccaatc agactttttg
     1381 ctacgtttaa tacattacta tcggaagaac tgcttgttcc tgttgtttct agacaataat
     1441 cggtcaagtc tttacttcct gcgtttgccc cattttctaa tgtaattgaa gcgacgggat
     1501 cactgtcatt ctttcctagt tttgcgtacc attgattcgc aaaagtaata cgattcccca
     1561 tcattggtat ccctgcacgc tcaaatttca cttgccactg ttctgttgct tcctctacgc
     1621 tttttgcttg taatgcaact tgtgaagctt tttctgttgt gtttagttca tgaatcaaaa
     1681 actcaacttg tacacctaaa tctgaccact ctttgtttcg ctctttggca aaatttttca
     1741 aagcagtcgt tcgccctgcc gtccattgtg caatcccatg cccagataat ggatcgtttg
     1801 gtcgttcaat cgctgtggga ctcatactcg actcttgttg aaaattgcct aacatacccg
     1861 ctagaccctc taatgtcgta tttggaatat gtttttgtac ttcctttgca atttgaagcc
     1921 cgtttgtctc catgccttgg ttcgttaatg agatagtact tgcgacaatt tcggttggac
     1981 tacagtcgat ttcttcctct tcagaaccac tactaatcaa aagtatcata aacagaaaac
     2041 caaacataca tagtaatagt gtgggtagac aaccgaaccc acaccataaa agaagttttt
     2101 tcttcatgtt tgatccttca ttcgcttagt tcattttttg tgttgctttt cttttgcgcc
     2161 aaaaagcaaa accgcttcct gaagccaata atccaccaag tgctgtcgca acgagagaca
     2221 cttcttcgcc agtcttaggc aatactttca tttttccatc agaccctttc accgcaatat
     2281 ttccgcttgg aagttgttcg accttgtctg aaatgggcgt caagccattt cctgattctt
     2341 tataagcgac accatcaacg acggagacaa ttttttctcc tgaagctgtt tcaattggtg
     2401 tacttggtgt tggaatatgg gttaattcag acgcattatt ggtttcaatt tgaacatttt
     2461 gtccacctgt ttgttggctg ccaatacttc catctggttt tactacgacg tcctgtactg
     2521 gaggcgtagt gttcgtcccc ccattattgg taggtggtgt cgttggagga gtcgtatttc
     2581 cttcattgcc tcctggattc gacggctttt ctggtgtctc tgtattacca ccatttcctg
     2641 ggttttcctc aacgggtgga acttcaggtg ttccttcccc tccgccagta tctggatttt
     2701 ctggtacttc aggcgtttct gtgcctccac ctgtatctgg attttcttcc gttggtggaa
     2761 tctctggggt ttcaggagtt tctggtattt cgggaatttc tggggtttct tctcctccat
     2821 ctgttggtgg taaaacaggt tcttcaggaa cttcaggtgt ttcttccgtt ggtggaataa
     2881 tttctgtcgg tggtacactt ggatcacctg gtgacgtagt atctgcatac gcactggttc
     2941 ctaccatacc tagcaataca gttgcactaa ataaacttcc taaaatcatt tttttcatgt
     3001 gtttttcctc ttctcttatt tattgattta tttcaaaatc atctagttcg ggaagcgtgg
     3061 aaactagata atttttttcc tgttcttcca attgaaaaga cacttttttt gtttcttgtg
     3121 tctttttctc ggtttccaat gtaatgacat acgcaatttg ccaatgcttg ccttctttct
     3181 tcatttccca ggataaaata cttttgactt tttccttttg aggactgatt tgtttcgcaa
     3241 ctttttccgt tagaaacgat tgaagctttt tcctataccg ttcttctgcc atttcacccg
     3301 aataataatt cgtaataaaa taacgactaa atgtatcaat ttgtggttgt tttgtcgcaa
     3361 gttcttccgc aaatgcaatg cgttcttgta actggtgctc ctttttcgtt agttgttcaa
     3421 tctctctttg cgttgaaagt tgcataataa aaagaccaca agatacaacg acgataaatc
     3481 caaacaaaag ccattgaaaa gacatttttt tcttctgaag agagccgttg ctttgtgtaa
     3541 acgctgtttc tacagaaagt ggtgttttct tttccttttt gatttttgtt ggtcgacgtt
     3601 cgttcttttg gtctttcttt acttgataaa acttcggtaa ttcggaattt aaggtttgaa
     3661 taaaggtttg tgcttcttca cttaattgcc cattttctgt taaagattgt tcaatcaatg
     3721 aaatcaaatg aaactcttct actggaaaat catacgagcc ttcaaatact ggaatttcat
     3781 ggttttctgt gtagatttcc aaggtacacc agccaatttc cgctcgtgcc aatagctctt
     3841 cattttctct tttcaattga ataaatgcct tatctaatgc aattttttta tctgttagta
     3901 aagacatgtt atcaacttct gtaaatcgaa tcatcatcat tagtcaaaca cttcttccac
     3961 cacttctacc gcatacacca cttgaatagg gttatttaaa gaaaatttca cccgatattc
     4021 ttgattgttc aaactgacga tatagctatc ctcatcttca gcataatagg tatattcgtt
     4081 gcctttgact tgaaaatatt tttggatcgc ttgtctttgt tgccattcgc caatctttgg
     4141 aaaataggcg aaatgatagc caaataaaac gcctatccct aataaaacaa ttcctgttac
     4201 tataatcatc tggtttcctc ctgtacctgt ttatcttcta ttgactcttt tgtcggtaag
     4261 caataaagaa ccagtaacac aagagagcca atcgttggga caaaatagaa aaaaataaat
     4321 gcccaatgaa aacctgcgtc tcgtaaacgt cgaacgctga gagaaagaga cgggacaaga
     4381 actacgaaac aatacacaat caatacgcct atcaaacaac caattattgt ttcagaagca
     4441 ttcaacgctc ctgctagtcc aatcgctaag cctaggccaa acccaatgag ggcattcatt
     4501 aacgcaacga gccaatactc ttttcttgtg gctttccctt tgtaattaaa gctattctgc
     4561 caaaattttt tataatttgt gatcatatct ttgtcctttc taatgtttat ttttgattct
     4621 agtttcttct gttttactaa caatgaaact tgccataaaa aaggtggcca atacaccacc
     4681 agctagaaat ccacttacaa ataacaaaat tttttcctcc tttctaaaaa atttcgccat
     4741 aaaaaggcaa agtatgaaca cgataaatac catgtacata ctttgccttt ttatgcaata
     4801 tatgttaact ctccaagcaa ttactgattt ttaaattctt tataaataaa ataaaaatcg
     4861 tttgagtaaa gaaataagtc aatgaagacc caaacgataa aaaatattcc taatactata
     4921 tttaataaac tacgttctag atagatacga gatttcaaat taaaaaacga aaatgagaga
     4981 tacaaatata tcaaatattt aaaaagtaac cacattttct ataccactcc ttatcagaaa
     5041 cagccttatt ctttagaata agactgtttc tgggttgatt aatttgtctt ttgttaaact
     5101 accatctaag gcaagttcta aatgtaaatg tggtcctgta ctatttcctg ttgaacccac
     5161 ataaccaata atttgtcctt gttcaactgt ttgtcctacg ttcacgcaat attcctgttg
     5221 gtgcgcataa agagccacac ttccgtcttc atgctcaatt gtgacataat ttcctgacaa
     5281 agtatgatac gaagcttcta tgaccgttcc tgccttgctc gcatagattg gttctccttg
     5341 attggctgcc atgtctaatc ctctatgaaa ttcttctcct cgccaaccga aggaactaga
     5401 aattgaataa ttttttagag gtaccatata gccttctttg cttagttgtg gaccagctac
     5461 ttctttatca tatttgatta agtcataggt ttcgattaac ccatttaatt ttttatcgta
     5521 gctagtgtct gttgcgtatc ttcctgttaa aaatttagtc gcttcctgat aggtttttgc
     5581 cttacttttc caaacaccat cataaaaaga ggtatttcca actaagcctt cttttaacag
     5641 tttggcatag tcttttaagc tttcttcata gctctcatat tgccgaaaag ttgcttgggt
     5701 cgtataaagt gtcccattgc ctaaatcttc ttgtgtcgca aaagagacgc cctttccgtt
     5761 atgtgtgcct ttgattccaa atagattgta gtttggtgcc tgagccaatt gactttgtcc
     5821 gcttgcggat tctaaaatgg cttgcgcgat cataacagaa gcgtataaat cattttcttg
     5881 tccaatttta cgagcagatt caccgatttt acggataaac gtttctacag acgcgtcttt
     5941 ttcaaaatga atagtccctt cgttatcttg tgtgctggaa gggactaatt cttgtatcgg
     6001 tgcgcttggt gcttggtggt agttttttgt ctcttctgaa ccgattgatt gtgcctgcgg
     6061 tacgtcattc ataggtacag tcacactctg ttcaggtgcc ttttgtgggc gttccgtttt
     6121 tgttggcggt tccgcttctg tttcggtact tggtttttga ggagtctgtg agctttcatc
     6181 agaaggttgc tcatttggtt cttctgacgg ttcctcctca ggttgttgat tgtcttctgg
     6241 tttgtctggt tgattcggtg tttctggttg ttcttcagaa cctgttggtg gagcgttcat
     6301 ttctggatta tctggtgtat ctattgtatc ttcagtcggt gtttctggtt gattttcttc
     6361 tccagtggaa tttgttgtcg tttcttcagt gatcgttgca gattgcgaat ttgtcagttg
     6421 attgttgcct tgttcattag ccaaaaccgc tggcgtatac ccaaatactt gacctactaa
     6481 taacaaagca gataaacatt tactcgtttt tttcacaaga ttgcctccat tcttcaatca
     6541 atcatctaga gacagctgat tgctgtctct agtcatactt taattgcttt gtttcatttt
     6601 acgacgaacc agaacagccc ccacaccaac aaaaagaaac agtcccaatg caattaacac
     6661 atcgttcact gtttctcctg tttttggtaa gctttgtttt ggtgtgctcg tcggcgtttg
     6721 tttgacaggg gttgtcggcg tttctttttt aatgcctttt gctgtcaatg tttgtttttc
     6781 attcttcaaa tcaaagtgac gtgcttcttc ctctttatgg tctttatcct tatagacaat
     6841 atgagttgcg accaatttct ccccgtcttt taacgtgcct gctttgtagt caaaggatac
     6901 tttaaactct tcagacgctt tagaaacttt acgtgcagtt tctaccgtac caacgactgt
     6961 atcttttcca tttttatcaa tcttatggaa ttgagtcacc cagtatttgg tttcccctac
     7021 cgtagattct ggaaattctt gcgatacatg atcgatcaac tcttggtctt tgtctggatc
     7081 aaatgtttgg tcgccattca ctgtaataaa gcgtgtttcg atttttggtt ctttcgcagt
     7141 cactgtttga tctttattgg ttaaatcaaa atgacgagcg aattcttctt cctcttcttt
     7201 gtcgttatag acaatgtgcg ttgcgacaat cgtgtcaccg tctgaaacca tgtctgcttt
     7261 gtagttgaac gtcacatcaa atgtttcttt ttcatcggtc acttttcgtg tttcttttac
     7321 cgtatctaat acagtgactt ttccatcggc agtaatttta tggaattgcg tcacccaata
     7381 tttctctgtc ccaaccgcag atttagggaa tacttgggac actttatcca ctaattcttg
     7441 gtcttttttc gggttgaaat cttttccacc tgtcgttgtc acaaacaaag tagaaatttc
     7501 tggttggtct tcttctggaa ccacgatttc ttcgtttttc acagtaattg gttgtacttc
     7561 tccgtctttg accgttacta aaatcgtttc tgtttgtcct gacgcttgcc cataatgctc
     7621 agggaacaca atttccgtaa atttatacgt accgtctggt aattttagtt tttctggttc
     7681 ttcttttgtt tcatacacaa aggacacatt tttattatgt gcagtcatac cttcaatcag
     7741 gaatttcgct cctggtaatt gtagatgatt gattgaatcg actttattga agtctacttc
     7801 acccgttttt aacaagttgt caatcacatc taattcaaac gtaagcacag gcgtcgtgtt
     7861 atcgccatag gccacagaga acgtggtttg ttcttcagaa ggttgacggt agttgtctgg
     7921 cacatttttt tcaatcaatt ggtatttgtt ttgatcgcca ttgtagaatt ttttgtttgc
     7981 cttccagtgt ccgtctttgt cagtttttac tgaatccaag acttctcctt ttttcacaag
     8041 gactgtttct ttatctggcg cagtaatatc tgttttggcg ataatatcaa attcgatatt
     8101 ttctgcagga acggtatcat aaacaggttt gccttgattc caggattctt ccgcattata
     8161 gacttctttg actttctttc cttctggaat gaactgttgt aagtcatttt ttgcagttaa
     8221 cgtcacttct ttgtttggtt caatcgtaat cgtatgagtt tgtttgtcta gaatataccc
     8281 agctggtgct ttgacttctt tgacatccaa tttattttga acatattttc cgtctttttc
     8341 atagaatgca gggatttctt ttaaggcgat ttcccctttg gcatccgttg tatattgttt
     8401 tgtttctccc gtatcatgac gtttgatttc atacgtcaca tttggaactt ttttccctgt
     8461 gacagcgtct agtttggtta gtttgaagtc gccatttttt agaactttaa tagttagtcc
     8521 agctctatta ggatctggca cataaagctt tcctagattt tgagcaacag gatctacata
     8581 ggcaaaactt gaacctaccc ctctagaatt tggatccagt gaagaaacag aaattcgtcc
     8641 gtcatttgag ttttgatttg cttttataaa caaactattt cctgattttc ttacttgaac
     8701 gttagctgtg ttttcaacaa ccgacccatc ccattgagaa aacacacctt ttgtatcctt
     8761 aacttctact tcttccccta ctttaaccgt cactgtttgg ctatcaaaag aaggatcttc
     8821 aagtaaattg tttatttttc tatctacagt ctgtttgaaa gcttcataat tagattgtcc
     8881 aatagttcca taattaacaa atgaaccacc taaaacttcc caaatatatg tttgtgctaa
     8941 tacatagtta tccattgaac cactagaata gtagccaaaa taaccaatta aagaaatctt
     9001 ttttctaata gcacttgtcg taattaaatt ttcgagtggt gtcacatcat attgaatacc
     9061 cgcactcgtg attgcgcctt cttgaatgca aaaaacatct tctccgtcaa tttgccagcg
     9121 aaccatgtaa tcatgagtat acattgttcc ttcaggagta attcgagtca tattcacatc
     9181 tttacgacca ttttcagtca atgttagatt ggtagcacgt gtctgtgcat tcttttccaa
     9241 gattgcttgc cattcttcag attcttgcgc caatcgttct tgttcagaaa aagaagtcgc
     9301 tttttgaata tcttcaatgg ttataaagta tttctcataa ccttcaggga cctcttgagt
     9361 cattacattg ggcggagaaa catcttctgt cgtgtcctgc gtagttgtct cttctgttgt
     9421 ttcacttgtg gtttcagtac tgatcgcttc ttcactactt tttgattctt cagtcttcat
     9481 tacttcttca gtagaatttg attcatttat cgtttctgca aatgccgtta cagatcctaa
     9541 catgggattg agaaatgtaa cgaaaattgc caaccaagtt gccattcttt taaacatatt
     9601 tttttcgttc ctttcatttt atttttcagt cttctaaagt aatttcttat ggacttcgac
     9661 accgaaacat ttttatcctc ctcaaaacaa aaaaagacta gaacttataa ttctagtctt
     9721 catcaaccac ttacttttct tttattttta gtagaaatct acagtgtgtt tttgtgaccc
     9781 gtcactgtaa ataataacat ccaccataaa acctttgttg taccactgcg aatttggatc
     9841 gctaacctct gcattagccc aatcataggc ttcatctaca gtgttaaata cctttccact
     9901 attaccaatc tctccaacta caattgttgg ctcttcaggt gtcgttgtgc caccatttcc
     9961 gccttgatcg cctccaccag gattgcttgg ttcttcaggc gttgttgttc ctccacctgt
    10021 gcttgggtta gaaggagttg tttctcctcc agtatttcca ccattacctg gttgtgtagg
    10081 tgtttgagta gtgccaccat tgttttctgg tgtacttggc gttgttgtac caccagttgt
    10141 tcctgtttga tctccagctg gtgttgttgg tgtcgttgta tttggtgtcg tttgtgtatt
    10201 tgaagtcgtt ccttgtccta ctggttgatt cggatcaact ttgtctttct cttgaatcac
    10261 tgtttgagcc acagtttcac ctgcgctgtt ttgtacactt gctacattgc cattaaatac
    10321 taatttattt cctgcataga ttaaatccac atttgtaatc ttattcatgt ccgctaattt
    10381 ttggacagta atatttgttg cttgactgat tgcgcttagg gtatctcccc agacaattgt
    10441 gtattcattc cctttaattt ctgctttgat ttgttccgcc gaacgaggtg tccaaacttt
    10501 gttttctgct gcttcagcat tcgttcccac agcaagacta actcctccga ctacgattcc
    10561 tagtgccaat actttcaaca ttttcattgt ttttcgcctc cgatttgaat aaatttattt
    10621 acaaaaacat agtaccaaac ttatgtctat cagtcgatat attttttgta gattttttta
    10681 ttttttgata tactaacttt ggtactaaaa taactaaaag gaatgtcaca catgcaactt
    10741 aataatttaa atgaaataca tgattgggct caaaataatt tacttaatcg taaacaagct
    10801 gccaaaatca ctggacaaaa ctatgatgct ttttctagct ctttacgtag aaataatatc
    10861 aaaccatttc tagaaatagg cgacgaacct ggtccttcca tcattcgctt atatctaaga
    10921 gaagatattg aaaaatacgc aaaacagctc gaagaaaaaa gacagaaacg tcaatgaggc
    10981 gttttctgtc ttggatttac aagtaaaaca ataataaaca cttttacaac ggaggttcta
    11041 tgaataagaa atacattgtt tcagttggtt caatcgtact gattgtactc gctattattg
    11101 ctttttctgt atccaacaaa acagaaacac agccacaaaa tacggttcaa tcgactacta
    11161 caagtcaatc tagcgatcat tccgtctatc cttcactaaa tagtttaaaa gaaacgcatg
    11221 gacaagattt aattgatctt ggttcttctc aaattactac tgtcttattt gaaaagaaaa
    11281 atattgcgtt tattactcct aaagaaggaa ctgaccttga attttctatc aaagtaacaa
    11341 ataccaaaga aaatttattc tccattcctg acattcaata tacccctaac aagttaaaga
    11401 ttggggatac gtttggtttg gctaaagtaa atgaacaata ctttgcttac tcactgaaat
    11461 aagtttctaa tagcaagagg aaaaaccctc ttgttatttt tatcacataa tcattttata
    11521 aaaacgcttc gaaaatatca acatttttga agaaactatt tttctaattt ctcaatttct
    11581 tccaagggat tttcgctaat gacccataca ggcacagatt gctctcgagg ctccccttct
    11641 tctaaccacc aacctgcata atgaacttct tcctcctcag cattaccatg ataccaacca
    11701 acaaaggatt catctacaac gaatgaaatt tcttcttctg gatcaaataa ttcatttttc
    11761 catgcttcaa ttgcttgttc tttggtgtac ttttgtttgt taaatacacc acaaatttcg
    11821 tcatctacat aaaaaatttc aaaatcaaaa ttactcgttc cttttactgg catcttaact
    11881 tttcctcctt tgactcactt caatagatat accactcatg ttcattccat tgtttactag
    11941 ttaattggag ttttatctta gggctttgtg cactctttcc aaaaacttga gcgtcgtttt
    12001 tttcttctaa ccaaaaaagg taatttctta cattcggtac taaatgtttt tcctgcattt
    12061 cttcatgaaa aacatataat tctgcttcat ttttcaacca attcattatt cattccctca
    12121 ttcacttctt cataccgcca ttcagatagt gagatttctt tttgtttctc cacttgtcct
    12181 ataaaaatcg ctgtattcaa caagctgatc acacttaata ataaagcaat gaaactaatg
    12241 gttacactta ctctttcaca ttttgtcatt ttgtttcctt ctttactcgt ttgttatttc
    12301 ttgtaatagg tctaataact ccttcaccga aatttgcaaa tggttcgcaa aaatttcaat
    12361 aaaagcactc gcacacatta ccagaccttc aactgatata tcactgcgac aataaaattc
    12421 tggagaaaca ctgatttctg attctttctt acaattgaac gaaaaaacta tttttgaatc
    12481 aaattccggt aattcttcca caactatgtt ttgggattct accaagaaag ggagtgcctg
    12541 ttctaatgct tcttctggtg tttgagcaga tagaataatt gcttcttgtt cttttatttc
    12601 attgttctca aatactttat aacttttttt cattgttagt tcctctttca ttcctctctc
    12661 aattagctaa gccgttactg gtacgccatt ctctaaattt agtatcgcta atccttaatg
    12721 ctcgtcgaat atcaatcatt cgataaccta ttgcacgaag ttctttatat cttttagcag
    12781 ttagcgtctc taacgtcatt tcaggcaatg ggttcgaatc aaaaatagaa ttcgaaccga
    12841 ttatttgggc caattgttgt acttgtaaca gctcttctga acttaattca gtgattgctc
    12901 gattgcctat cattgcatac attttctgtc gaatatactt cttttcttgt ttagacaaca
    12961 tttctatcca ctcctatttt cattatcatt tgttacctcc acttcagtaa atccacaaat
    13021 aaatgaattc atattccaag taccattccc ttcaactaca acttttctcc tgtcctcttt
    13081 cggatattca agaatcagac cgtttaccaa tatgtttttc actactacta atttgtttgt
    13141 ataccgtgga attgctgttc cgatatactt cactttattt cctacgctaa ttgtcatttc
    13201 ctcactccta ttctttttta tccaaaactt tttcctgtcc tattcctgcc aacgaatata
    13261 cttcactgca tgcttaaaca aaacaaaatg ttgccctgtt ttatttccgt cttttacttg
    13321 ttccacatac agttcatatt tggtcacacc ttttaaaacg ccagatactt tttttcatcc
    13381 attgtcacaa ttagtttttt catcaatcga ttgatactgc actgctcatt tttcttgttc
    13441 atatagtatt cctttctttt tcaaaactag cctcttttgt ttactagttt tcatcaatca
    13501 tcaacgattt aacagatttt ttctttcgca gggtttctag cgtcagcaac cccaaactct
    13561 aaaaaagtgc ctttgctccc ttgaaaaacg agcggacgat tgtactcgtc atcatatgtt
    13621 tttcatacta cctgatccac gcaaataacc tttttttgtt ctgtgccaca cccatccata
    13681 cgtatcgctt gagattcacc atactttttt aggtggttta tccttcctaa gtccctgtta
    13741 gacacgctga ttaacttaga aagtcctgtt tgtatccaat tttcaaagat ctcccaaaag
    13801 aaataagtag gtttacgtgt atcgcttctg ttcaaagtac ctgtttgtac ttgagtattt
    13861 ctactcccct cgttcaaggg attccttcca ccagctagct tttcctttca tcatcaaatg
    13921 ttcctgaaaa gcatttacgt ttcgtgtccg tgtgcttact tattcttttt tcgatagcga
    13981 ttatccgctc gctactcgct attttatact ttgtttgtta gctcaaataa aaactaacaa
    14041 acaaaaaagg ctagtttctt tccgttttat ttttttaact gtcgataaat agttgttagt
    14101 ttaattccaa gttctaaaac caatacgccc aacaaaacca caggaaaaac aattggggca
    14161 aacaagtaaa atcggatagt tttcatgtct tccctctttc tttttggtgt cgctgtggtc
    14221 gttcttgccg ttcaatactt cgatacattt tttattcatt atttgctcgt tttgttttcc
    14281 tctggactgc ttttcttgct ttttgaatac tttatacgct tttcgtgcct gaattaatag
    14341 gtcttgtcgg cgtttcattc gtgagaaaac aatatttgaa accatcaaat cctggttcga
    14401 acaaccacga ttcgcaataa ttttcccatc aaaaaaaata gttttcactt gagaaaaaaa
    14461 cagttggaaa tcttgttttg ttgctaattt ttttgtcaaa atcactgtgc ttttccctgt
    14521 tgaatctaca atgattcttc gaacaatccg attcgataac ttccaacgtt gccgattaaa
    14581 caatgttact tcacaacaaa tatgacgtaa gtgattaggg gtcattttca tgtgtccaat
    14641 tcctccaacc aattatgcat cgaaattttg ggcaattgtg acagcccata aatctgcgtt
    14701 tccaggtctt cataactaca agaacgcttg gcttgaagta atgaatattc tgcttgatct
    14761 gatagttttg ttgccattgg atctagattc aattctctta cacaacattc ccaaaaattc
    14821 aatagcgctc taaaataaaa tccttttcga ttctgaattg cttttccttc atgacgagca
    14881 attttttcat tttgaataca ccgttgatac gttttgctta aatcttctgc aaatgtttcc
    14941 cctaaaattc gatactcgct tccatcagat aaaccatgtg tttgattata ctttcgttgc
    15001 cgcttttcta taattcgttt actgtcgtat atcatgttta gctgctcttt cgcttcacgc
    15061 cattcagctg aactgcgaac aatccatgtc aaaacatctt tcggaacaaa cgtactttcg
    15121 ctgtaatgag caataaactc tttttgccaa tcatcagcca tttcaacagg actagtcgta
    15181 tcgatttttg tatctagatt ttctgtatct agattgttag aagaattatt aaataattgt
    15241 gcagaatttt cacatggatt tttttcgtca aaatcatcga aaatcgctaa attatcaaca
    15301 gattcctcct cttcttttcc ttgtgcagaa tctacactgg ttgagtcaac aggacctaat
    15361 gaatacacat cattttctgt cgtatctaat tctcctagat acaaccgagc tttgtttctt
    15421 tttcctgttt gttgatcaaa tcccatatcc tctatttcta gtagctcata ttctttcaat
    15481 tcttttttga ttgtattaac agaacgtctg caacaattca atgcattggc taattcttgt
    15541 tccgtaaatt caaaataaac aaaaccttga ctatctactt gatttcgttg aatggctaaa
    15601 tctaagcgtg accgaaaaac catatacgcg atttttgctt tatcagacaa tgagcgatat
    15661 ttttcacttg taataaatac ctttggaaat tgatagtata attcattgta tttacgagtt
    15721 gcggtaaatc tttttgttgc catcgttatt acacctcatc ttgtccatat attaagaaag
    15781 aattttggtg ttggtagaca cgaacatctt ccattgtagc aatcaattct tttagataaa
    15841 ttcgaccgaa ttcaattgtt agtaagtcgt aatcttctaa ttcatttttg atttcttcaa
    15901 tttcttcttc agacacattg agtaaaatgg ctaattcttg attggtataa gaaatataga
    15961 catgtttctt ttcatcaatc cgtttttcaa ttaaggattc ttctaagtac tcattcagta
    16021 agctataggc gtattttgct tgtgcagtca tcgcataata ttttgacgat tttataaaga
    16081 atagttttgg catatgaaaa gttgcttgtg tcagcacatc tgttgcgcga aatttgtttt
    16141 ttaccatatt ttttcctcct agaaaaacac gcaaaaaaag ctctgtataa atcgaattac
    16201 acagagtttc ttcttgttga tatttaattt ctaaaatacg tttcgggtaa aaactaaaca
    16261 cttactaata tttagattgt agaatcttcg ccttcattta ttcattttaa agagaaaaag
    16321 atcaagtgaa atgtgcccaa aagtttgatt ctattaattt ttaaacacca ttcataatat
    16381 atttcctata atgtaaaaag cgtcgatact tattaaagca tcgacgttat ttttatagta
    16441 aatttatcaa aatttttgcc aacatttttt ccgatttttt tccctatttc tatctttcct
    16501 tatctttctt agttaatatt atttgatgtt aatatatcac tactaatatt aaaaaacatt
    16561 gatttatcat aggataaaga gtgatagttt aatacttaaa gagacatatt aattttattt
    16621 aaaaattgac attaatgact cttaatcagc gggtcgcggg ttcgagcccc tcacggccca
    16681 ttgggtgcca aacccacgga acaggtaaat gttggtgcga aagcatttcg aaaacattta
    16741 cgctgttctt tttttatttt tcaaaataac tagggctttt tatttttaac tcaaagtcca
    16801 ctgtagccct ttggactttg agttaaaaaa gaagcataat tatgaacagt aaagtttcta
    16861 atataacaat aattcgagaa gctttgcaaa atcatgctgc caatgaactc tctaaacata
    16921 taggattaaa tctaagtact ataaaaggtt aaaaagtgaa gaatatttaa tagaaaaact
    16981 aaatttactt gctgctttcc ccttttccat taatacaaca atcagagagt gtcttttttt
    17041 cgaaaaaatt agaataaata aaagacacgt agttttttta tctacatgtc ttccatcgac
    17101 tacaagttat catttctcaa aacgtattta gcttttaatt gattaatcgt ttcacgttgt
    17161 ctacacattg cctaattaag attttcttat tagaagcatc aatcccgcaa taatacctaa
    17221 aactccagga attacccaca ataatgttaa aagaaaaaat ggaataatag ataaaatgag
    17281 taaactaata ccatagacca ctgttttttg attttttctt actaaaaaac ctattatgaa
    17341 aatcattatc gaaaaaacta gggtaattcc tccaaataaa acaacattcc cgcttagatt
    17401 gactaacata tctatatcct tactggttag ttctgtagta tttaaagtag ttgaatcaat
    17461 agaagataga aacataccac ccatcaatgc taaaagacta cataaaatcc ctgttattcc
    17521 actaattaaa atcaaaataa actctgtttt tctcttattc atttaattct ctccagatta
    17581 agtgttttag aattagaaat tttttgacca tagttaatag cttctaatga acgaataaca
    17641 accactgacg tgttagaaaa ataatcccca atatgttgtt tcttgggcgt gaaagcagcc
    17701 accaaatacc caatttgaaa tacaaaattt tttaatataa acctacatat acactcacga
    17761 ataaagatag tttgccaact tagctctttt tcctcaaagc tcactactct tatcccaaaa
    17821 atcattttcc caattgtttg tcctgaattc agcttagtca aaagtacaaa ataggttaaa
    17881 taaatagcta atgacgctat accataaaaa gtaataatgg atttgtcaga ttccagtccc
    17941 atgattttgt aaaagagacc tatagtagca tgggtgatga tgctaataca aattaaatca
    18001 ataagaaaag caaaaaatcg taaccaaaat cccgcaaata cataattagg caaagtttca
    18061 atgccactct tcttattcat tttatagccc tccatagtaa tacattggtt ttggagtatc
    18121 agaaaatcca agtttttcaa aaactgaaat tacacgatct gatgtagttg cctttagccc
    18181 ttgccattca gcaatcttat tacctaacca agaagaagca aagccagttg tgtcattttt
    18241 atattctata accgtcgcat cagttaattt cttctctttt tttagggaat ctaatgcatc
    18301 ttcagagtaa ccaatttgat ccaccaaccc attttctact gcttggttac catcataaat
    18361 tcttccatcc gcaagttctt tcactgcatt agtatccata tttcttcctt ttgctacaac
    18421 attgacaaaa cgatcataag cactcatcac aaattcctgt ataactttat tctcttcctc
    18481 tgaaggtttg tgttgaggtg acatcatatc ttttaatgca ccacttttat acgtcgcatc
    18541 agttacgcca agtttttcta ataagccaga ataatttaaa ccagaaataa tgacaccaat
    18601 tgatccagtt gttgtttctt ccgtagcaaa tattttatct gaaccagcag aaatgtagta
    18661 ccctccactt gccgctgtat ttttaaatgc agtatagata ggaatatcta gctttttaat
    18721 ttttgccatt tctttagcaa tctcagcact ttcatatatt cctcccccag gtgaatttac
    18781 ttctaataac actcccttaa ccgctttgtc ttcttgaatt ttctttagct gtgttaaaaa
    18841 attctgatgg ttatattgtt ccctactaaa aagattgctt tcacctgtat ctgcaataac
    18901 tccattgacg gatagtttga caatcttttt atttgatgcg ccttcttcta acgtttcttc
    18961 tgtgatttcg ttagatccgt aaaaaatctt atttattcct gttagggttg tatttgatgc
    19021 ttcctcttta tcttttcctg gaattagcgc gacgataatg gatagtaaca ataatccgca
    19081 cgctactccc acagcaatcc atcttctttt attcatataa actcctccta tattcttagt
    19141 aaaaataatt attttttatt tagaaacaca tcgacaattt gttgccaata tgttttttct
    19201 cctacaatta aagacaactc tccagttaat ccataacgaa acggaaacga ttgagttgtt
    19261 tttaacgttc ctttcactgc ataaaaaaca cctttctcag tggcagtaga attttctgaa
    19321 atttcatcta aagttccgtc gatcgtaatc ggagccccac ctttttgatc caatttaaaa
    19381 tgaaccttca tcccttcttt aatataggta ctttcattcg caggtaaaag tgctgtaaat
    19441 ttcatttttg atttttcgtg gatcggataa atttctgcaa gcactgttcc tttggctagt
    19501 tctgcttgtc cctttgtttc ttcatttaaa tgaaccgttc cagatacagg agcttttagt
    19561 attccttttg aaagttgatc gtccaaactt ttgataactg actggttctt ctcttgtttt
    19621 tcatttagct ctgtaactgt ttgttttaca gttgttattg cttgttctag ctcttgcttt
    19681 cctttttccg tttgactaat tctgtcattc tcagaagtag ttggtttaac taatttagct
    19741 ttttctgact gatactgttc tacttctttt tttagttgct caatttgttc atttatgctt
    19801 gctgaaattg tcagtttcac ttgatttttt tgatcatcag aaacattatt tagttgttct
    19861 tgccaattct catagtttgc catcatttct tttgaaaaat cttttaatga ttgattatta
    19921 ctccaagcta cctgtacttg ttgccattct tgtaatttac tatctgaact ctcaatttgt
    19981 ttttctagtt gtctttttgt cttttcgtat acttctaatt gcttttgatc atttaattca
    20041 ctttgcttca aagcgtagcg gagtgattct ttttctgaca acatgctctt taattgatta
    20101 ctgtagccaa aactgtcatc cgttgaaaac aaattttttc ctttacttat actttcaata
    20161 aatagctgag ccatctttat ttgttgagta atacgttggt tttcttgttc tatctgagcc
    20221 ttttcattct ctattaaaga acaatccaat ctaaccaaca catcgtcctt atgaacaaat
    20281 tgattttctt ttaggaagtt ttccattatt ttagcttcta atggcacttg taacttctct
    20341 attttttcgg ggttcaattg agccgttgtc cgaataacaa cctctttttt agcaaaggtt
    20401 aaaaacaatc caataataca taaaaaaaga attacagggt ataacaccca acggtagaag
    20461 ctatgatgtt gtttagtata gatactagaa tgttcgaacc aattattatt ctgcataaat
    20521 tgtttccctc tcctagattt cccataattt ttgatagata ccatcggcaa aacgaagttc
    20581 atcgtgcgtt cctttttcta ccaaatgccc ttcatggaga actaaaattt gatcacatgc
    20641 ttttgctatt ggtaaatgat gcgcaataaa aataatcgtt ttttctttta aattgaggag
    20701 ataattcata attgcatgtt ctaacaaggt atctaatcca ctagttgctt catctaaaat
    20761 taatatgtcc ggatttttta ataaagcccg tgctaaggct aatcgttgtc gttgccctcc
    20821 tgaaagatta tttccacctt cttccaacaa tgtctcgtaa cgtaatggct gttgattaat
    20881 aaattcattt agttggacta tctcacaaac ttctattatt tgttcgaaac ttggtgggct
    20941 atctaaaccg aatataagat tctcaatgat tgttccacta aagaaaaatg actcttgagg
    21001 caaatatgtc actcgatcgc gtaatatctg aaaaggaatg tctaagtgat taatgtttcc
    21061 ataatggatc gatccttctg atgcttcata aaaacgaaca aataactttg caagagttga
    21121 ttttccagaa ccactaactc caactatagc cgttttactg tgcgaaggaa ttgtacatga
    21181 aatttcgttc aacgttgggg ccttcatgct atacgagaat gaaacgtttt ttacagtgat
    21241 atcttgttga aataattgtt cagaaagcat atattcaaaa gactggttcg atttttctga
    21301 atcaatcgcg aaaatttcat ttaaacgatt atttgcaact tgagccgttt gcattttcac
    21361 ttgtaaatta ataatgtttt gtaaaggatc tgtcaaaaat actagtaaag cattgtatgt
    21421 gataagttga cctagactaa ttctattatc cataacaaaa taagaaccta gccataaaat
    21481 taatgcactg ctaattaatt gaatcaaatg tttaattcct tgttgaatat tatcaagcgt
    21541 aaccgtacga aatgattttt tcattaattt aacaaattca tggtctatac ggttatatac
    21601 tttctcttca ccgctatatg ctttaattgt ttctattcct ttaagacttt ctatgatact
    21661 agaatttaca gctgcaccgg ctgccatttc ttccctgttc gatttttcat agcttttcac
    21721 gaaagcgaat attgcaacta cataaaaagg caaagagcat agagtgatta aaaacaacgt
    21781 actattctga atagctaacg taaccccaac aagcaacacc ataccaatat ctaaaaatat
    21841 tgataacgta gcacttgcta aagcatcaat aatcttactg gcatcaagaa aacgagaaat
    21901 aatttcccct gattttctcg tagcaaaaaa attcattggt aaagttaaca catgtttgaa
    21961 atactgaagc ataattgcca tactcattcg ctgtcctaaa ataataagaa gataactacg
    22021 actatattca aacaataccc gaaataaata gacagcgatc aagccaatgg aaattatatt
    22081 taaagttgaa cgtgcttgat tagggatgaa aaaatcaaga ataccttgaa aataatatga
    22141 gcttcctatg ccaaaaaacg taatgaaaat cgatgctaaa acgatgtgga atactaatcc
    22201 tttttgtttc caaattatag gcaaaaaaga acttaatcca gctacttttt ctttaatagg
    22261 aatataagtt tctctaggca taggtaataa aagaatacct gtccattctt tttgaaacgc
    22321 ttcaatcgtt tttctatttt ttccattagc gggatctgca atccatagta cgtttccctt
    22381 cgttttataa attacaacgt agtgcatata gctgttatca accaatatat gtgcaattaa
    22441 tggtaagata agatctcttt ctttccagat ctcactatct gcttgaatag ccatacaatc
    22501 aaaagacaat ttttcaaatg tctttttcaa tccaaatgct gaagtaccct ctaaatcagt
    22561 cccagatagg tcccgtaatt tatgaatcgg gatatcagtt ttaaaatatt ttaaaatcat
    22621 cgaaaggcaa gcaacaccac aatctttttc atcttgttgc tgcacaaaag gaaatgattt
    22681 tatcacttat ctaacacaac tccttctaaa aaatttttat agaaaaattc tcaatatagc
    22741 tttaatacag tgaaaatatt gtctcataat tccacaaaaa ctttgaaaac agttcttgaa
    22801 cactaacttt ttcattcctg aatcaaattt attttggaaa ttttactatt caagaataaa
    22861 cttgataagt ttcaggaaga tttttactgt taatcaaata cgtatgttac agtagcaccg
    22921 taaaaaatat tatttaaaga agcgtgttta aacatgcaaa atgtaaagga actaagtatt
    22981 gtggaaatgc acaaactgtt ggtggaggca ataatgcttg gggaaaattg gggcaagttg
    23041 taggtggact taccacaggg gcagtaggtg gtgcaggttt aggaacagca atttgcggac
    23101 cagcttgcgg tgttgttgga gggttatatg gagctgtcgc tggaggtgct gcagctggct
    23161 gggatgctcg aaaaaaataa aaaaggagaa taagtagtat gcatattaaa aacacaaaaa
    23221 caacatttat attatcttca gaagagttga aaaatattca aggtgggagt gccgtgggag
    23281 ttttaggcac tactttttct ggggcaacag caggagtaaa actttgttca gccggaggac
    23341 cttatgcaat agctgcttgt ggtgtaggtg gagctttatt aggtgctgga ttcagcatgt
    23401 ggaccggtgc gtaaacaatt aaaaaatatc aaacctcaag attggtttgt gtcgattacg
    23461 aacataggta tgttctttat cttttcaaaa gtttggaatg gacagaactt tttactaaag
    23521 cttcttgtgc tagtttttat aattatatta aatttttatc ttttattaaa gtcagataaa
    23581 aattctttgg aaatgtaatg gtagagatat ttttacaaaa ggacttgcag cgctgtagtt
    23641 ggtgctgcag gaggacctat ttcggccgtt acaggaggta ttggtaatgt acttgtcgga
    23701 tgtgcattaa agcctgttcc tgcaagttaa atttataaaa acaggaaagt aattatctca
    23761 aatcctatca agtacaatca taggattgca cttgacagga caaatttttt aactaaaaaa
    23821 tgaaaaacac tttctaaaaa ttagtagaat ttgccaaatc tttcgcatct tccaccatac
    23881 atttaatttt aattcccctt tatggtactt aataaaaaca agaaaagaac tattcatttg
    23941 gccctttttt ggttccagac catagaaata ttcctccagt aactaatatt gctgttatgc
    24001 acgtaactaa taacagctat aaaaattggt acaactttaa taggattcag aaaaatttgc
    24061 actagtgaag gaatctattt tattagaaaa atgcaatctc cctaaagaaa aaaccactaa
    24121 acagtgcttt ctttcaactt ctccttaatt ttttgtgctt tccctaaagt aaagtttctg
    24181 gccaactctg aatcaaaata tagtgaacgt tttttaaaat ctatttcttt aacgttaaag
    24241 atatttacta aacacgctcg actaattctt gttaacatag gatatttctt ttcaagctca
    24301 ctaattcttc cataaaattc gtactgccca tctttagtac acaatgataa tctatgtggt
    24361 aattttgaag cttctacaaa ataaatgtca tttatatcaa aagtaaatgt ttgagaacct
    24421 atggaaaaaa caaatgcttg attcgtaacc tgttttgttg catctatcct ttcttgcgct
    24481 aataacaata actcaacaat ctcgtttcga tactcatcta gtgtttgatc ttttgttaca
    24541 aatcctaacg tttcaactct tctttttatt gttacgggta acatctcatc atgagtagtt
    24601 gtaaaaatga tttttgcttg aacatcatat tttctgatta cttctgctaa ttctataccg
    24661 ttaacttcat gatttaaatc aatatctaag aaatatattc cttgctttgg tcggaattgt
    24721 ttgaggtatt ttttaacttc taacggactt tgtgttttca atactatctt aaatacttta
    24781 tcgtgaaaaa gaatgaaatt atcaataatt cgttctaact gatttaattg tataatttga
    24841 tcttcacata atattattgg ataactcata aatcctcctt ccctcaaaac aacaaaataa
    24901 cttgagtagt aaattgatac ggatcgtttt gatagttcac aaacatattg ggattctttt
    24961 tattgatttc ctctatacta gataaaccta acccttgatg tccctttttt gttgttattc
    25021 cttttttcat tagagtatct atagagagta gtatctctct acaactattc gatatcgaaa
    25081 tttctatctg tttatcatct cggtaaatag ctagagacag ttctctatct tgactctcta
    25141 tagatgcttc aattgcatta tcgagaataa ttccaagcac cctaatacag tcaaatattg
    25201 gaacaggaac ttcaataata gtacttggac attcgaattt acaacaaatc ttattgttat
    25261 ttgcttggta tagcttcgct atcaacaaac ttttcaaata ttcatttttt atatttttta
    25321 aataacgata ttcaaactta ttggttagat aagaattaga atagatctct aattctttta
    25381 tttctttttc caatatcgaa tcatggtttt gattagcgat ttcttttaaa ctcagcaata
    25441 aatttttata gtcatgtcta aatctggcaa atttttcttg atctttttcc agtgaatcag
    25501 tatatttttt tagataaacc aattcttttc ttttcaattg ctgttcatat tgttctcgtt
    25561 gcctaatagt tattctaata aataaaaata gaacaacaaa catttgaatg attaaaaata
    25621 ttaagacacc aaacacaaaa tgatcaaata cttgatattg atgagcaaca taagaaacaa
    25681 ataaaccaat tgtaagtaaa taaatcatga atatagcagt aagtttagaa ctggactgtt
    25741 caacaatcct atttatagct aattttctat ataaccaaat aaacatcagc aaaaacaaag
    25801 aatcaagcat tatctggaat actacataac catacccttt tatcccttca aatgaaaaca
    25861 tatacgtcat aaccatggaa gtaataatag atctgaaatc atcaataagc atacataata
    25921 gtaatttatt taaaagcact atattagttt tcttcttttc ctttaatata aaataactt
//
LOCUS       DARBDV010000033        25619 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000033 DARBDV010000000
VERSION     DARBDV010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.033"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            127..3654
                     /locus_tag="SCM56_001291"
     CDS             127..3654
                     /locus_tag="SCM56_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEG1110102.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3651..7373
                     /gene="addA"
                     /locus_tag="SCM56_001292"
     CDS             3651..7373
                     /gene="addA"
                     /locus_tag="SCM56_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEG1110103.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7442..7783
                     /locus_tag="SCM56_001293"
     CDS             7442..7783
                     /locus_tag="SCM56_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1110104.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8092..9135
                     /gene="pheS"
                     /locus_tag="SCM56_001294"
     CDS             8092..9135
                     /gene="pheS"
                     /locus_tag="SCM56_001294"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEG1110105.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9140..11560
                     /locus_tag="SCM56_001295"
     CDS             9140..11560
                     /locus_tag="SCM56_001295"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEG1110106.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="SCM56_001296"
     CDS             complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="SCM56_001296"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEG1110107.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12994..13731
                     /locus_tag="SCM56_001297"
     CDS             12994..13731
                     /locus_tag="SCM56_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEG1110108.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13745..14560
                     /locus_tag="SCM56_001298"
     CDS             13745..14560
                     /locus_tag="SCM56_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEG1110109.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14573..15220
                     /locus_tag="SCM56_001299"
     CDS             14573..15220
                     /locus_tag="SCM56_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEG1110110.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15236..15892
                     /locus_tag="SCM56_001300"
     CDS             15236..15892
                     /locus_tag="SCM56_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEG1110111.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16054..16875
                     /gene="racE"
                     /locus_tag="SCM56_001301"
     CDS             16054..16875
                     /gene="racE"
                     /locus_tag="SCM56_001301"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEG1110112.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16878..18233
                     /gene="rph"
                     /locus_tag="SCM56_001302"
     CDS             16878..18233
                     /gene="rph"
                     /locus_tag="SCM56_001302"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEG1110113.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18234..18752
                     /locus_tag="SCM56_001303"
     CDS             18234..18752
                     /locus_tag="SCM56_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEG1110114.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18859..19359
                     /locus_tag="SCM56_001304"
     CDS             18859..19359
                     /locus_tag="SCM56_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEG1110115.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19639..20559)
                     /locus_tag="SCM56_001305"
     CDS             complement(19639..20559)
                     /locus_tag="SCM56_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEG1110116.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20724..21737
                     /locus_tag="SCM56_001306"
     CDS             20724..21737
                     /locus_tag="SCM56_001306"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEG1110117.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21847..22602)
                     /gene="fabI"
                     /locus_tag="SCM56_001307"
     CDS             complement(21847..22602)
                     /gene="fabI"
                     /locus_tag="SCM56_001307"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEG1110118.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22822..23262
                     /gene="fabZ"
                     /locus_tag="SCM56_001308"
     CDS             22822..23262
                     /gene="fabZ"
                     /locus_tag="SCM56_001308"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEG1110119.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23377..24138)
                     /locus_tag="SCM56_001309"
     CDS             complement(23377..24138)
                     /locus_tag="SCM56_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEG1110120.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24327..25421
                     /locus_tag="SCM56_001310"
     CDS             24327..25421
                     /locus_tag="SCM56_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEG1110121.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            complement(25507..>25619)
                     /locus_tag="SCM56_001311"
     CDS             complement(25507..>25619)
                     /locus_tag="SCM56_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG1110122.1"
                     /translation="SVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT         8485 a         4439 c         5405 g         7290 t
ORIGIN      
        1 cgtatactta aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag
       61 aacaaatgtt cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg
      121 aaaagcatgt ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg
      181 atcgacgaag catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt
      241 cccaactaca gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc
      301 ggaaaaaaaa ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg
      361 tatttgctgc aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca
      421 atgatcttgc gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa
      481 atcaataaaa gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga
      541 aatattcaac cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag
      601 caacttaaaa tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa
      661 cgtgcactaa caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat
      721 ttcaacaacg tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa
      781 ttactgcagg caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct
      841 tatacagcgg atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc
      901 cgtctttttc aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca
      961 gagcagactc gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc
     1021 caaaagaaag cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct
     1081 gaaaatccaa ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa
     1141 gaaaactacc gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat
     1201 atccttcggc cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg
     1261 atggaaaatc atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct
     1321 tatcggctaa gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca
     1381 gaaaccgaac ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc
     1441 actgaaaatg tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac
     1501 tggcagtttg tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa
     1561 gaacagtcaa atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa
     1621 ataaagaaaa gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa
     1681 agtggcgtac agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta
     1741 gaagcggcaa gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat
     1801 gtagtaattt atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt
     1861 ggtctagaaa atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat
     1921 cgtttggaat tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat
     1981 caagtgtttc cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta
     2041 ttaaatcagg ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct
     2101 tttgagcctt tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt
     2161 tatgcacaaa gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc
     2221 acagaatatt tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct
     2281 aattgttatg tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta
     2341 gctttagatg aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg
     2401 aattcgtcat ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt
     2461 cctgtttcga tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca
     2521 atttcacgta tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg
     2581 aaattgaaag aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat
     2641 gaatcattgg atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg
     2701 tccgatgcgc agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt
     2761 cggtttgaga tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag
     2821 acgattcaaa aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct
     2881 cttcagacag agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa
     2941 ttaccgttga gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta
     3001 gcttctgaac aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca
     3061 ttcgatgtca cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta
     3121 gcattgatgg atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat
     3181 tatttacatg ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg
     3241 aaaaaattta gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat
     3301 cattcactgg aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat
     3361 gaataccaaa aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta
     3421 ttcatggcgc ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa
     3481 atcaaactaa atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc
     3541 agaagcatct gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa
     3601 atagataagg aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa
     3661 ttcctttaaa accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc
     3721 aaggagataa tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc
     3781 gaagagtgat tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat
     3841 ttactgaagc tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg
     3901 tcaatagcga aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc
     3961 ccacagcgaa tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt
     4021 acttaattga tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga
     4081 tgaaggagga tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat
     4141 ttttccaatt aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg
     4201 tgttttctct ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt
     4261 taagcgataa ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa
     4321 tccgtccttt agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc
     4381 agttagcaca aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc
     4441 agatcaagaa tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt
     4501 tggaacaact aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt
     4561 ccaatgaagt caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa
     4621 gttattttcc tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag
     4681 tcgaagaaat gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg
     4741 aaaaaggtgt gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa
     4801 aaacgaaaga tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag
     4861 aagtaatggt agatgaatat caagacgtga accaattaca agaagccatt ttatactggc
     4921 taagagaacc cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca
     4981 tctattcttt ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa
     5041 agaaagaagg cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc
     5101 tttcttttac gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt
     5161 atgacgaagc agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg
     5221 agccggaaat catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg
     5281 atgatatact ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc
     5341 aattaattga ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag
     5401 agtacaaaga cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga
     5461 ttttcaaaac attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga
     5521 cagaaattcg cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc
     5581 cgcttgcggc tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca
     5641 ttcgattagc taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa
     5701 acgaagatga attagcagct aagctggaac attttggcaa gcaactagag cattggcgag
     5761 aacttgctcg ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat
     5821 atctagagta tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat
     5881 tggttgatcg tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc
     5941 gtttcattga aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag
     6001 aagataatgc tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg
     6061 tatttttgtt agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt
     6121 ttgaagaaaa acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg
     6181 atacgttacc gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa
     6241 tgcgtaaatt gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct
     6301 ataaaaataa agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag
     6361 tttttgatcc tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg
     6421 gattgatccg tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc
     6481 ttcaacattc aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac
     6541 aaagacagtt gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg
     6601 aaactttatt agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt
     6661 cttcacaaac cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag
     6721 atgatacaca agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc
     6781 aatttcgtta tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag
     6841 tgtcagcagc tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga
     6901 tgccaactac agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag
     6961 acgaaaaagt cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac
     7021 ttggtcaaca gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc
     7081 tgcttcctgc tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc
     7141 acggaatcgt cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa
     7201 cagattttat tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc
     7261 aaggacagct aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa
     7321 atgttttcct aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat
     7381 tgagcaaact tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat
     7441 gatggagcta acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat
     7501 tttaggaaaa aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg
     7561 ctttgttgag cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt
     7621 gaaggaatta gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg
     7681 ttcagaatat ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca
     7741 agtttggagc gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct
     7801 tgtaaactat agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta
     7861 aaccagttca gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt
     7921 tcactttccg agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt
     7981 gcggtgtaag tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca
     8041 ggaatgaggt ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta
     8101 caagcacaat tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat
     8161 ttaaatgaat taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa
     8221 gtattacgtg ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca
     8281 aatgagatcc gagatctttt gacagaagag atcgagcgaa aaaaacaagt attagaagaa
     8341 gaagcgttaa atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa
     8401 gtaaaacatg gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt
     8461 gtgggtatgg gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc
     8521 gaacggatga atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt
     8581 tcagacgaga tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa
     8641 catgatttct ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat
     8701 acagatgatg cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa
     8761 aacatcacaa tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg
     8821 gaagatcgtg aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa
     8881 gtagatgtca gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga
     8941 tggatcgaaa ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc
     9001 gatccagaag aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg
     9061 cggtatggag tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag
     9121 ttcaaggggg agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt
     9181 atcgaatgta acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga
     9241 gggagtaacg gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg
     9301 cgtaccacat ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga
     9361 actgtctcaa atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc
     9421 acttcctaac tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca
     9481 agtatcaaat ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc
     9541 gaaagaatac gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt
     9601 ttttccttat ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc
     9661 ggatgcattg tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc
     9721 tacattcgat gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt
     9781 ggaagttgaa gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag
     9841 aatccagcca agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat
     9901 caacaatgta gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc
     9961 atttgactac gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga
    10021 aacattagta acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac
    10081 taacggcaaa gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac
    10141 gaatgaaaca acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg
    10201 tacagcaaaa caatttaatt tgcgtagtga atcctctgca cgttttgaaa aaggaatcaa
    10261 ccatgcgact gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg
    10321 tggggaagtt ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt
    10381 gaagatttca cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt
    10441 aaatgagatt tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt
    10501 agtagtacca ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc
    10561 tcgtatttat ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg
    10621 aagcttgtca aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg
    10681 attgagcgaa gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat
    10741 aaatgaaagt aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg
    10801 aatgaatttg atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac
    10861 agagattgct ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca
    10921 tctgccgcaa gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga
    10981 ttggcagacg aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt
    11041 atttgaacaa ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga
    11101 aatgcatcct ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg
    11161 tcaagtgcat ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt
    11221 tgatttatct gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa
    11281 attcccagca gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga
    11341 attaacagaa gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga
    11401 tgtctatcaa ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt
    11461 cgtcaaccca gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga
    11521 aaaagcgttg gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta
    11581 acgatcaagt agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca
    11641 catctctttt tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct
    11701 ttacagcctg cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat
    11761 tttacttcag aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag
    11821 atttccataa cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca
    11881 aattcagctt ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg
    11941 cggcggtaat gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag
    12001 taggtgaaga ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga
    12061 tcagtataca tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata
    12121 ccgtttccta acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt
    12181 ccttttggtg ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag
    12241 ctttgtacga tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca
    12301 agattcgctt tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct
    12361 gtaggcacgc tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc
    12421 cgaacgcgtc ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga
    12481 atttgtgatt ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct
    12541 aaagcaactt gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt
    12601 gcttgcatgt cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact
    12661 cgggttttta gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc
    12721 gttaaaataa tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa
    12781 tatcttaccg tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg
    12841 ataaaataac taatagatta gaaatggaag aaatatttat gggagaataa acgttctaag
    12901 aaactttaca aattactaaa ctatcagata taatgtattt tattatacat ctttaaaaga
    12961 aatcttacta aataataatg ggagtgtaat tctatggcaa tgattaaatt tgatcacgtg
    13021 gataaatatt atggcaagtt ccacgcatta aaaaatatca atctcgaatt cgagaaagga
    13081 gaagttgtcg ttgtcattgg tccttctggt tcaggaaaga gcacgatgct tcgctgtatc
    13141 aatggattag agacgatctc ttcaggtaag ctgctgatta atgatactga cttgcatgat
    13201 aaaaagacaa aactgacaga agtccgaaaa aacatcggca tggttttcca acatttcaat
    13261 ttgtacccaa ataaaactgt tctggaaaac atcaaattag caccaatcaa agtgttgaag
    13321 caagaccagg caactgcagt caaaaacgcg gagaaatttt tgaaaactgt caatatgtta
    13381 gataagaaag attcttatcc ttccatgtta tccgggggac aacagcaacg ggtggcaatc
    13441 gccagaggtt tagcgatgaa tcctgagatg ctgctgtttg atgaacctac ttctgctttg
    13501 gatccggaaa tgattggtga tgtattagac gttatgaaaa aattggctca tgacggtatg
    13561 tcaatgattg tcgtgacaca tgaaatgggc tttgccaaag aagtggcgga tcgtgttatc
    13621 ttcatggctg atggagaagt attggaagac agcagaaatg tgagagactt tttcgaaaat
    13681 ccaaatgaag tccgagcaca gcaatttatc agtaaagtta tcaatcattg ataggaggaa
    13741 aaagatgcgc aggttaaaaa aaatacgcct gattttcctt ttaggcattg cttttcttgc
    13801 actagccgga tgtaaaggaa atgggttggc aaatgaagat attttagagc gaagcaaatc
    13861 taccaatgaa atcatctggg gtgtcaagta tgatactcga ttatttggaa tgatggatat
    13921 tgagagcaga actgtacaag gatttgatgt agatattgca aaagcaatca ctaaaaagat
    13981 ccttggtgac aatggaaaaa cagaatttgt tgaagtcact tctaaaactc ggattccatt
    14041 attgaaaaac ggaaatatcg atgcaattat tgcaacgatg acgatcacag atgaacgaaa
    14101 aaaacaggtt gacttttcag atgtctattt cgatgctggc caagctttgt tggtaaagaa
    14161 aggaagccaa atcaaaagtg tagatgattt gaatgcttct acaacggttc tagcagtcaa
    14221 aggttctaca tcggctgcga atatccgaca gcatgcacca gatgcaaaaa ttttagaatt
    14281 agaaaactat gcagaagcat ttactgcttt acaatctggt cagggagacg cgatgactac
    14341 ggataatgcg attttgttag ggattgcaga tgaaaatcca gagtatgagt tagttggtgg
    14401 aacgtttact aatgagcctt acgggattgc gatcaacaaa gggcaagaaa acttcttgaa
    14461 agctgttaac caagcgctag aggaaatgca tgcagatggc acatacgata aaatctatca
    14521 aaaatggttt ccaaatgaaa ctgaaggaaa agtagagtaa gggggagagg caatgagcga
    14581 attgatacaa acttatggac caatctttgt cgacgggttc aagatcacat tgttttcgag
    14641 tgtcttagca cttgtgttta gtttgattat cggaacgtta atggcaatct tccaattatc
    14701 aaagaataaa gtagtgagcg ccttagcaaa ggcatatgta gaatttttta gaaacattcc
    14761 attattaatc atcgtgatgt ttttttatgt tgtcgtaccg atttattggt ttaatctaga
    14821 tggattccaa gcaggtacga ttggattaac gatctataca tctgctttta ttgctgaaac
    14881 agtacgttca gggattcaaa ccgtaccaaa aggtcaaaca gaagcaggac tatcttctgg
    14941 ttttacctat tcagaaatca tgcgttatat tgttttaccg caagcattca aaatcgttat
    15001 cccgccatta ggcaaccaat ttatcaacct cgtaaaaaac tcatctattt tagcaatcgt
    15061 agctggttta gatttgatgt atcaaggaga cttgatcgct agtgaaacat tcaatacctt
    15121 cgatacgtat atcatcgttg ggttatttta cttagttatc acgctaccgt tatcttactt
    15181 aatggtttat cttgagaaaa aatgggcggt tcgttcatag aaggagggga aatacatgga
    15241 ttttagcggc gctttttcat ggatgaatat ccgtttttta ttggaaggat taaaggttac
    15301 tgttgaagtt tcattgtttt cgatcatttt tagtttcttg atcggtggtc tgacaggtgt
    15361 gattcgtttt gcgaatatcc ctttcttttc caaaatcctt ggtgtagtga ttgatattat
    15421 acggaatctt ccattattat tgatcatttt cttcacttac tttgccttac ctcaaatcgg
    15481 cattcagatg aacatcttct ggtccgctgt cgcagcatta acgatttttg agtcagctat
    15541 gttgtcggaa attttccgag ctggactcaa tgctgttcca aaaggacaga tggaagcagg
    15601 attatcaacg ggactgactt atgtagaatc gatgcgaacg atcgtgttac cgcaagcatt
    15661 taaatcaatg atcccggcaa ttgtgagcca attgatttca ctgatcaaag atacttcatt
    15721 agcagtaatc atttctttac ctgaactttc tcatcaggca cgtattattt atggacagaa
    15781 caccaattat gtgcttccga tgttcatcat catgacactt atgtacttcg tggtatgtta
    15841 cgcgctttct ctgctttccg gttacttgga aaagagacgg tacagttatt aatagatgat
    15901 tcttttagcg gttgtggcaa actaaagtca caaccgcttt tcctctttcc attcgaaaaa
    15961 aagagatttc gattgtttta agcaagtaag agcacttgaa aattcacgct aaaaagtaca
    16021 caatagggag gatagaattt attgaaaaaa aggatgatac gattgacaga taatcgccct
    16081 atcggattta ttgattcagg tgtcggcggc ttgactgtag taaaagaagc cctgaaacaa
    16141 ttaccgaatg aaaatatttt atttgtagga gacacagcac gctgcccata tggccctaga
    16201 cccgcggaac aggtaatcca gtatacttgg gaaatgacgg attatctggt ggagcaagga
    16261 atcaagatgc tggtgatcgc ctgcaatacc gcaactgcgg tggctttaga agaaatcaaa
    16321 gctgctcttt ctattccagt catcggtgtg atccttcccg gtactagagc ggcagtaaaa
    16381 aaaacacaaa ataaacaagt tggcattatc ggtacgattg gtacggtaaa aagtcaagct
    16441 tatgaaaaag cactgaaaga gaaagtacca gaattgactg tgacaagtct tgcttgtccc
    16501 aaatttgttt cagttgtcga aagtaatgaa taccattcat cggtggcgaa aaaaattgtg
    16561 gcagaaacat tagctccttt aaccactaaa aaaatcgata cattgatttt gggatgcacc
    16621 cattatccat tattacgccc catcattcaa aatgtaatgg gagaaaatgt tcaactgatc
    16681 gattctggag cagaaacagt aggtgaagta tctatgctgt tagattattt caatttgagc
    16741 aattcaccgc aaaatggtcg gacattatgc cagttttata caactggctc tgccaaactt
    16801 ttcgaggaaa tagctgaaga ctggcttgga atcggacact taaatgtaga acatatcgaa
    16861 ttgggaggaa aataaggatg cgtcatgacg ggagaaaagc ccaagagctt cgcaaactgg
    16921 agcttcaaac aaatgttttc aaatatccag aaggatcggt agtgatccgt tttggtgata
    16981 caacagttat ttgttctgca acaatagaaa attctgttcc cccttttttg agagaaacag
    17041 gaacaggatg ggtgacagca gaatatagta tgctgcctcg agcaacgagt acgagaaatc
    17101 gtagagaaag cagcaaaggg aaactttctg gtagaacaat ggaaatccag cgtcttattg
    17161 gtcgttcctt gcgtgcagta gtagacttgg aaaaattagg agaacgtagt atcatcgtag
    17221 actgcgatgt gatccaagcg gatggcggta ctagaacagc aagtatcact ggggcatttg
    17281 tggcattgaa attagccatt gaaaaacttt taagagaaaa agaattgtca gaagatccaa
    17341 taaaagaaca tcttgctgca gttagcgtag gaatcttacc agatgggact tgcgtcacag
    17401 atttggatta ccaagaagat tccgcagcac ttgtagacat gaatcttgta atgacagaat
    17461 ctggaaaatt cgtggagatt caaggaactg gagaagaagc aacgtttgat ggtgaacagt
    17521 tgaatgaaat gctctttttt ggaaaaaacg cgattgaaga tttgatcaaa gagcaaaaac
    17581 atgctttgct aacagagttt gctcaaaatg atgagcggat agaagaaaca aaaacaatca
    17641 tcattgcgac aagaaatcca ggaaaagcag aagagtttcg aaatatgttc aaagaagcgg
    17701 gatatcatgt aaaaacatta cttgactatc ctgaattgcc agatgtggaa gaaactggca
    17761 gcacatttga agaaaatgcc cgattaaaag cagaaacgat tgcacaattg cttgatcagc
    17821 cagtcttagc agatgattca ggtttgaaag tggatgcttt aggcggtatg ccaggaatct
    17881 attctgcacg ttttgcaggg gaacaaaaaa gtgatgcggg gaataacgcc aaattattat
    17941 atgaattgac agatgtgccg gatgaaaaaa gaacagcaca attccattgt acgttagtat
    18001 tcgcggcacc caaaaaagac agccttgtag tagaagcgga atggcccggt cgagtagccc
    18061 gtataccatc tggcgaaaat ggctttgggt atgatccttt atttatccca gaaggtaaga
    18121 aacaaacagc agcagaatta tcgagcgaag aaaaaaacaa gatcagtcat cgggctcaag
    18181 caatgaaaaa attgagtgcc gagtggaaac aatggctgga aggagagaga tagatgcggt
    18241 atttagtagt aagtgataat catggagaca gagatatcct cgttgactta gtagatcgtt
    18301 atcgttctga agtggatatc tttttccatt gtggtgattc ggaattagaa gcttctgacc
    18361 cattatggga tacattccaa gtggttcaag gtaattgtga ttatggacca ggattcgaac
    18421 agaaaaaagt gatccaaact ggtcaggata cagtattcat gactcacggt catttatcga
    18481 atgtccgatt tggtttgaca cagttagcaa tcgaagctcg agctgcaact gccgatatgg
    18541 tgttttttgg tcatacccat caaataggct gtgagatgga tcagaaaatc ctgtttttga
    18601 atcctggtag catttctcag cctagaggcc cgatccaaat cccatcatat gctcttatcg
    18661 aaagtttaga tgaccaaatt tctgttcaat actataaccg tgcccatcag gcagttgaag
    18721 agttggcctt ttctttttcg agagagcgct gaataaaaaa actctaattt ttaataaaaa
    18781 taagctttat tttatgaatt ttgcatccgg tttcggtaaa atagtagtgt tgaatgaaat
    18841 agaaatggag gccatcagat gattggacct atagtaagag aaatgctgct ggaaaatcag
    18901 gaaacgtttt tgattcctgc agaaaatgtg gcaaatgtta tgtatcatca tccattgtcc
    18961 catggtttac ttgtgttatc gaaggtcggt tattccaaaa ttcctgtatt aggaaaagat
    19021 gatcactttg taggactagt aagtttatca gatgtggtca ataaaatgat tgacttgcaa
    19081 acaatcagca tggacccgtt agaaggacta acggttgctg atgtgacaga aacagatgtt
    19141 ccgacaattg atgaaaattg ggaactagaa gaagtactcc atttgttagt cgattcgcct
    19201 tttttaccgg ttgtcacaga agatcggatt ttcaaaggaa tcattacaag aaaagagctg
    19261 cttaaagccg taaacttcat ggtgcatgaa ttagaacgtc gtaatatatt gtctccgaaa
    19321 atcgaagaat taaaagaaca aatcgatatc gtgagctaaa gaacgagaaa ctaaaagata
    19381 atcaaaaaat aagtagaaaa aacagcgtac cagttttgac tttatcttga aaaagaggct
    19441 gggacaaaag cgtttaactc caagaaataa gaaggaattc acgaaaattg cttttcaaat
    19501 ttttgtgaat ttcagcttat ttccgaagga gttgcttctg cttccgccgt ttatacgtgt
    19561 ttagagcgtg ggacaaaagt gttctttact tttgtcccac gctcttttta gtgagattcg
    19621 ttgcatcgtt tgtccaaatt attttccgac agcaattgga ctgttcggaa tcgtgaaacc
    19681 agaagctgtc aattctttga cataggagct caagaactct tcttttaagg catattgttt
    19741 tccgttcaat gcgtagcaga ccgtacgaat cgcaaaattg ctgtttccaa gatccactaa
    19801 tccaaagata gaaggttcag tttggagctc ttcttggtat ttttcagcta atgtacgatt
    19861 tactcgatca atgatctcat agattttgtc atatccttct tcaggaacaa tccgaatatc
    19921 taaaagtact tgcatatttg cacgagagag attgctgatc gtggtgatgt ttcgattcgg
    19981 gatgaaatgg acagtaccat ccgttgcttt cagctgaagg gtacggatac caacagatac
    20041 gacggttcct tcaatcgtta agtttgctag cttgatgtag tccccaacat ccatctgttg
    20101 ttccatgata ataaaaaatc cggtaataac atcactcatg aacccttggg caccaagacc
    20161 gattgccaca cctgcaattc cggcaccagc caaaagtgat ccgatcggca caccgatagc
    20221 agataaaatt gcatagataa aaaagaaacc aagggtataa tgaaaaatag tatttaaaag
    20281 actatgtaaa gtctttaagc ggctctcgtt tagaatggct tttttagaat actgattaaa
    20341 agacttatca atcagatatt ttcccacacg gtgaagaatc caaaaaagga cgatcaaaaa
    20401 aatcagatat aaactttttt ggataaaggc agccaaaatg gcgtcccaat caatcccgtc
    20461 ccaaaaccgc tgaaacgcat ttactttgct gacagcttga tctgtcaatg tctcgccaga
    20521 agagtctgtc gtaccggcag ttgctaataa tgaaaacata aaatcctctt ttctaaaatt
    20581 tgattctttc tgtattgtac caagaagttt tcaaaaaagg aaatagcaaa aataactata
    20641 atgaaggaaa aaagctgaaa ataaaatgac aaataatatg gataatgata aaatgtggag
    20701 aaaataacga aggtggtaag tatatgcaaa tcgattggaa caatctaggt ttttcttata
    20761 tcaaaactcc ttggcgtttt attgccaaat ggaaagacgg acaatgggaa gaaggagaat
    20821 tgacagaaga taattacctg acgattcatg aaggctctcc agcgcttcat tatggtcagc
    20881 aatgttttga aggtctaaaa gcttatcgtc gaaaagacgg caaggtcaat ctctttcgac
    20941 ctgaacaaaa tagtcgacgg atgaatcaaa gtgctagacg attgctgatg ccaatggtcc
    21001 ctgaaaagat gttcgttgat gctgtcaagc aagtggtaaa agcaaatgaa gattttgtgc
    21061 cgccctatgg aacaggcgct acattatact tgcgcccttt actgattggg gtcggtgaaa
    21121 acattggtgt ccatccagct ccagaatatt tatttgtcgt cttctgtacg ccagtaggag
    21181 cgtatttcaa aggtggacta aaaccaacga attttattgt atctgattac gatcgtgctg
    21241 ctcctaaggg gactggtgca gcaaaagtcg gcggaaatta cgcagcgagt ttacttccag
    21301 gaacagaagc aaaagaactg gattactcag attgtgttta tcttgatcca gccacacata
    21361 cgaaaatcga agaagtaggt gcagcaaatt tctttggaat tacaaaagac ggaaaattca
    21421 tcactccgca atcgccctct atcttaccaa gtatcacgaa atattccttg ttgcagttag
    21481 ctgaagaacg actaggactg caagcaattg aaggagatat tttcattgat gatctagatc
    21541 aatttgcaga agctggtgca tgtggtacag cggctgttat ttctccaatc ggtgggattt
    21601 atcacaataa caatcttcat gtattttata gtgaaacgga agtaggacca gtaacaaaac
    21661 ggttgtatga tgaattgaca ggtattcaat ttggagacat agaagcacca gaaggctgga
    21721 ttgaagtcgt ggaataattt caaagtaatg agttgaaaaa aacgtaaaaa gaaaaaatca
    21781 ggaagagact ttaaaaagtt ttttcctgat ttttatatgt tttactttaa agaatgcaac
    21841 aaaagtttat gaaagatgga cacctttgtc aacgtagata atatcaccga tgattccgct
    21901 ggaaagtggg ctgattaaga aagcacaagc attaccgact tcttcgatcg tcacaccttg
    21961 ctgatcaggc gtacgagatt cagataactg gatcagtttt tgatagtctt taacacctgt
    22021 gacagctaat gttttgattg ccccagctga gattccattg acccgaattc ctttaggcga
    22081 gaactcagct gccaaataac ggatagcagc ttcaagagaa gctttcgcaa tacccatcat
    22141 gttataattc gggattgcgc gttctgaacc tagataagtc atgctaacga tcccgctgtt
    22201 tggtgcaaga aacggttgtg cgtattttga tacagcaatt aaagagtagc tgctgatatc
    22261 ttgagctaat tgatagccgt ctctggaaat atcagacaca tttccggaca attcttcttt
    22321 gttggcgtaa gcaactgcat ggacaaggcc gtggatctcc ccagcatatt caccgatggc
    22381 attcattgct ttttcgatgc tttcgtcgct agaaacatca cattctacag tgaatgcttc
    22441 ttcgccgacc aatttgacta atgatttttt cattcgttca ttttggtaag tatagataat
    22501 ttctgcccct tgtttcaaca ttgcttcagc acaaccccaa gcaatgcttt ttttgttagc
    22561 gacgcccata atgacgattt tttttccttc tagaaatgac ataatgactc tcctttgttt
    22621 ctgataaaaa tttgatagtc gtaaaaaaat agagaataat agctaaaata acttcttgat
    22681 ttcttaatta ttagtcaaaa tgttttgatt gtcaaactat tttttcttaa attaaaaaaa
    22741 gattgctaaa tatttaggaa tatgatactt tgatagtcaa agtaatgaaa atggcaattg
    22801 cctgaaagaa ggagtttaat tatgtcttac gtattatctg ctactgaagt aatggaattg
    22861 attcctaatc gttatcctat ttgttatatt gattatgtag atactttaga accaggaaaa
    22921 aagatcgttg cgacaaaaaa tgtaacaatc aatgaagatt ttttccaagg tcactttcct
    22981 gacaatccag tgatgccggg tgcgttgatt ctagaaacac tggcacaagc aggttcgata
    23041 cttatcctaa aatcagaaga gtttctagga aaaagagctt atatcggtgg gatcaataag
    23101 gccaaattcc gtcaaaaagt tgttccagga gatgtgctga aatgtcattt tgaaatcacc
    23161 aaaatgaagg gatcagtcgg tacagcgtta tctgcagcat atgtagatga taaaaaagtc
    23221 tgtgagtgcg agttcacgtt catcgtcaac caaaatgctt gactataaaa cctgatatag
    23281 caaaacaaaa ggaagcttac ttgcttttta tgtaaaaaga agacgcctga atcctagcat
    23341 acgttgtcat ctatgcagga ttcagacgtt atttttttat ttttgttttg cttcttgcag
    23401 tatattgatt ttatcgattt ttacttcttc ttttggtttg tctgaactat ctgtttcgat
    23461 atttgcaata gcatccacga catccattcc ttcaatcact tggccgaaga cagtataatg
    23521 gccatctagt gaaggataac cgccgttttt ataagcgtcg atgattgctt gaggatagtc
    23581 gtcttttaaa agaccatcat gcatatcatc tgtattttga acgataaaga actggctgcc
    23641 gttactgtta ggatcttgtg agcgagccat agataacgca cctcttatat tataaaggtg
    23701 gttgttgatc tctgtttcaa atccttctcc ccaaatgctt tcccctccag tgccatcgcc
    23761 tttaggatcg ccaccttgga tcatgaaatc tttgatgacg cgatggaaag tcagtccgtt
    23821 gtagtaaccg tcttttgcat gtgtcataaa gttttcaacc gcttttggtg cgtgttcggg
    23881 aaataatttg attttgattg tgcctttatt cgtcaccatt tcgaccagat cttcattttc
    23941 ggcgacatct ttcgtcaatt gtggtaattc caatgcgttc aaatcgacgc tactggcagt
    24001 cgtgctttcc gcagtagatg tactttttga actttctgta gacgaactag aagaagtcgt
    24061 gttgtttgca gaacaaccag cagcaaacag caatgctgct gctaaactaa tacttaaaat
    24121 aactttattt ttcttcataa ctgttccttt ccaatttact attttgttat ctttaatgat
    24181 aacaaaatag agggaaaata cctacttaaa ttattggaat ttatcattta ttttaaaaga
    24241 aaaagaaaag gtgtagacca tttactttgt gtttgctata atgtaagtgg atacaaaaac
    24301 gaaaagatgc aggaggaaat ggtaatatgg gaaaattagg aatctcgatt tatccagaac
    24361 ggtcaacatt tgaaaaggac aaagcgtact tggatttagc tcataagtat gggtataaac
    24421 gggtttttac cagtttgctg cagatcaatg atgataaaga aaaagttctt tcagaattta
    24481 aagaagtagt tgattacgcg aatagcctag gcatggaagt aatggttgat atcaatccag
    24541 cgttatttga acagttggag atttcttacg atgacttatc ttttttccat aagatgggtg
    24601 cctacggggt ccgcttagat atcggattca caggagcaga agaagcaaaa atgacgcgca
    24661 atccttttgg tatcaaaatc gagatcaata tgagttcggg cacgaattac gttgacaaca
    24721 tcatgagtta ttcgccaaac acggataatt tattaggttc tcataatttt tatcctcatc
    24781 gatactctgg tttaggatac gaacactttg ttttttgttc agaaaaattc agaaaatata
    24841 atttgaatac gatggcattc gtcaattcgc aatctgctga gtttggccct tggcctacgc
    24901 aagatggctt gtgcacgctg gaagaccacc gagatcttga aattgcgaca caagtgaagc
    24961 atttaatcct tacaggattg attgatgata tttctattgg aaatgcgtac gcaagtgaag
    25021 aagaattgaa agaaatggca gaagcattta acgcggatta tccaacattg aaagtagata
    25081 cggaagaagg tattacagaa aacgaacgaa tctgcttgtt tgataacctt catagttatc
    25141 gaggggatcg ctcagaatat atccttcgtt caacgatgac tcgtgtatac tacaaagata
    25201 aagatttccc gcctcataat acgcgagata tgcatcatgg ggatgtactg attgacaatg
    25261 aagggtacgg tcaatacaaa ggagaaacac aaatcgcctt gaaggatatg aaaaacgacg
    25321 gacgtgtcaa tgttgtagga agaatctccg atgacgaatt attcctcctt gactttttga
    25381 aaccatggtc aagctttaaa ttaattgaaa ataaaaaata gataattagt tattgcagaa
    25441 ataatccgct agaaatagcc agttatttct gcttttttat tttgtttttt tataggttat
    25501 gatttgtcaa attaagctag aataaaatgc ttcttgtaca tagctcaaaa atatttcgat
    25561 tggtgttctg taattcaatg attttcttgg aatatgatta cgttgattgc tgacactgg
//
LOCUS       DARBDV010000034        25535 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000034 DARBDV010000000
VERSION     DARBDV010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.034"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..845)
                     /locus_tag="SCM56_001312"
     CDS             complement(<1..845)
                     /locus_tag="SCM56_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG1110123.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDS"
     gene            complement(1069..3009)
                     /gene="thrS"
                     /locus_tag="SCM56_001313"
     CDS             complement(1069..3009)
                     /gene="thrS"
                     /locus_tag="SCM56_001313"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEG1110124.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3346..3525)
                     /locus_tag="SCM56_001314"
     CDS             complement(3346..3525)
                     /locus_tag="SCM56_001314"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEG1110125.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3572..5395)
                     /gene="cadA"
                     /locus_tag="SCM56_001315"
     CDS             complement(3572..5395)
                     /gene="cadA"
                     /locus_tag="SCM56_001315"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEG1110126.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5673..7952
                     /locus_tag="SCM56_001316"
     CDS             5673..7952
                     /locus_tag="SCM56_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1110127.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8045..8692)
                     /locus_tag="SCM56_001317"
     CDS             complement(8045..8692)
                     /locus_tag="SCM56_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110128.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8871..9590)
                     /locus_tag="SCM56_001318"
     CDS             complement(8871..9590)
                     /locus_tag="SCM56_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1110129.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9721..10872)
                     /locus_tag="SCM56_001319"
     CDS             complement(9721..10872)
                     /locus_tag="SCM56_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEG1110130.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11089..11829)
                     /locus_tag="SCM56_001320"
     CDS             complement(11089..11829)
                     /locus_tag="SCM56_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG1110131.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11833..12156)
                     /gene="rsfS"
                     /locus_tag="SCM56_001321"
     CDS             complement(11833..12156)
                     /gene="rsfS"
                     /locus_tag="SCM56_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEG1110132.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12213..12806)
                     /gene="yqeK"
                     /locus_tag="SCM56_001322"
     CDS             complement(12213..12806)
                     /gene="yqeK"
                     /locus_tag="SCM56_001322"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEG1110133.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12790..13440)
                     /locus_tag="SCM56_001323"
     CDS             complement(12790..13440)
                     /locus_tag="SCM56_001323"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEG1110134.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13450..13761)
                     /gene="yhbY"
                     /locus_tag="SCM56_001324"
     CDS             complement(13450..13761)
                     /gene="yhbY"
                     /locus_tag="SCM56_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEG1110135.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13777..14886)
                     /gene="yqeH"
                     /locus_tag="SCM56_001325"
     CDS             complement(13777..14886)
                     /gene="yqeH"
                     /locus_tag="SCM56_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEG1110136.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14883..15410)
                     /locus_tag="SCM56_001326"
     CDS             complement(14883..15410)
                     /locus_tag="SCM56_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEG1110137.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15590..16375)
                     /locus_tag="SCM56_001327"
     CDS             complement(15590..16375)
                     /locus_tag="SCM56_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEG1110138.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16368..17237)
                     /locus_tag="SCM56_001328"
     CDS             complement(16368..17237)
                     /locus_tag="SCM56_001328"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEG1110139.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17238..18617)
                     /locus_tag="SCM56_001329"
     CDS             complement(17238..18617)
                     /locus_tag="SCM56_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEG1110140.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18625..19044)
                     /gene="fabZ"
                     /locus_tag="SCM56_001330"
     CDS             complement(18625..19044)
                     /gene="fabZ"
                     /locus_tag="SCM56_001330"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEG1110141.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19069..19545)
                     /gene="accB"
                     /locus_tag="SCM56_001331"
     CDS             complement(19069..19545)
                     /gene="accB"
                     /locus_tag="SCM56_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEG1110142.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19548..20783)
                     /gene="fabF"
                     /locus_tag="SCM56_001332"
     CDS             complement(19548..20783)
                     /gene="fabF"
                     /locus_tag="SCM56_001332"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEG1110143.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20802..21539)
                     /gene="fabG"
                     /locus_tag="SCM56_001333"
     CDS             complement(20802..21539)
                     /gene="fabG"
                     /locus_tag="SCM56_001333"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEG1110144.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21551..22468)
                     /gene="fabD"
                     /locus_tag="SCM56_001334"
     CDS             complement(21551..22468)
                     /gene="fabD"
                     /locus_tag="SCM56_001334"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEG1110145.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22575..22799)
                     /locus_tag="SCM56_001335"
     CDS             complement(22575..22799)
                     /locus_tag="SCM56_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEG1110146.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22852..23814)
                     /locus_tag="SCM56_001336"
     CDS             complement(22852..23814)
                     /locus_tag="SCM56_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEG1110147.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23824..24264)
                     /locus_tag="SCM56_001337"
     CDS             complement(23824..24264)
                     /locus_tag="SCM56_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG1110148.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24570..25535
                     /locus_tag="SCM56_001338"
     CDS             24570..25535
                     /locus_tag="SCM56_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEG1110149.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         7085 a         5630 c         4432 g         8388 t
ORIGIN      
        1 ttcgaatcca taaaatattc ttggattgct tctcgtgttt ttttcacttt ttcgctaagc
       61 ttttcgtttt tagccatata gaaataaaaa atgatatcga tcgtagcaaa ttgggccaaa
      121 agtgaattag ttgccgcact tcgattcgtg atttcttttg gtctagatgt ttgtacgagt
      181 acatcggcta tcttgctaag cgggttattt ccaaattgcg tcaacgcaat gatttcgata
      241 ttcatgcttt tcgccagctc ggctaatgcg actacatctg cacttctacc tgagtttgaa
      301 acaagccaaa gcactttctt tttctcattg ctgacaagtt gcgggagcaa gacgtggata
      361 tctttttcaa aaatgattgg cttaccgaca cgagaccatt tttgcagaat atcttctact
      421 actaaagaag aggcgccaac cccatataaa aaaacagtct ctgccgattc caaacactct
      481 acgacttttt ggatcgtttc ttcttctaga atatctactg tttcttttat cgctacttgt
      541 gcattgttac caagtttgtt tttgatagag taaaaggact catttgcttc tatatcacta
      601 aaaccttcat cgattccagt ctgtagttct gttgaaatcg tgattttgaa gtccgtcaga
      661 ctttgaaaac cgattttttt ggaaaaacgt acaactgtcg gcgcactcaa cccggaagct
      721 tctgctaatt cattagctgt cattgtaggg accttctcaa tattaccgat aatgtagttg
      781 gccaactttt tttctgctga agaataatct tccatcatac tttcaatacg actaattacg
      841 ctcataagtt cctccaccct tttaatgata gtctatgtaa gtataccata tcagaccctt
      901 attcacggtc gtatactgga taaaaaacaa aaaaacaatc cgcccaacag aatatcttat
      961 cccatcaatc ccacagattc tattgagcat ctgtgtcttt gaggaatacc tgttatctat
     1021 tgttcggatt gctttctatc tactactttt ttacaggaaa tttacctatt agttttctct
     1081 gctgtaattt ttgacttctg cttccatagc atcaataaag atattcaaat cttctacagc
     1141 tgtttcttta cttccataac gacgtacgtt cactgttgca tcttccaatt ccttgtctcc
     1201 aacaactaat tggtaaggga tcttttgtgt ttgagaggca cgtatcttat agcccatttt
     1261 ttcattacga tcatcaactt ctacacgcaa ccccttcatt tgcaaacgtt ctttgatttc
     1321 ataagcataa tcgccatgtg catcaacaga aacagggatg attgttgctt gtataggtgc
     1381 caaccatgtt ggaaaagcgc ctttgtatac ttctgttaaa taagcgacaa aacgttccat
     1441 tgtagaaaca attccgcggt ggataactac tggacgatgc gtattttccc cgtcttctcc
     1501 aacatatgtc aaatcaaagc gctctggcaa taagaagtct aattgtatag ttgataatgt
     1561 ttcttctgta ccaagagctg ttttgacttg aacatctagt tttggtccgt aaaaggctgc
     1621 ctcgccttct gcttcaaagt aatctagttc taattcatcc atagcggctt tcagcattgc
     1681 ttgggctttt tcccacattg cgtcatcatc aaaatattta tctgtattgt ttggatcgcg
     1741 atagctcaag cggaaacgat aatctgtaat attgaagtca gcatatactg ccaccatcaa
     1801 ttcaagcgta cgtttaaatt catctttgat ttgatcagga cgaacaaatg tatgaccatc
     1861 attcaacgtc atttctcgta cacgttgtaa acctgacaaa gcacctgatt tttcatagcg
     1921 atgcatcatt cctaattcag cgatacgaat tggtaattca cgataagaat ggatcgtgtt
     1981 tttgtagacc atcatgtgat gagggcagtt cattgggcga agaacaagca tttcgccatc
     2041 tcccatatcc attggcggga acatgtcttc ttgataatga tcccagtgtc cagatgtttt
     2101 gtacaactca acatctccca ttattggtgt atatacatgt tggtaaccta gactaacttc
     2161 tttgtctacg atataacgtt cgatcgtgcg tcggattgtt gccccttttg gaagccagaa
     2221 tggcaaacca gaacctactt ctggggaaac catgaacaag tcaagttctt ttcctagttt
     2281 acgatggtct ctttcttttg cttcttcacg gagacggata tattctttca aagctttctt
     2341 gtcaaaaaag gctgtcccgt atacacgttg catcatttga ttgtttgagt ttccacgcca
     2401 ataagcacct gccactgata ataatttaaa tacttgtata cggccagtgg atggaacatg
     2461 tggtccacga cacaaatcga tgaattcgcc ttgttgataa gcagtaatca cttcttcttc
     2521 tggcaattca ctaatcagtt ctactttgta cggatcactt gcaaaaattt ctaatgcttc
     2581 ctgttttgaa agaacacggc gttcgatcgg cagattttct ttgacgattt tcatcatttc
     2641 tgcttcgatt gccggcaaat cttctgcagt tacttggttt tgtccattat ctgtatcgta
     2701 gtaaaaccct gaatcgatag ctggtccaac gccaaagtgg atatttggat agaggcgacg
     2761 catggcttgg gccatcaaat gagctgctga atgacggact aagccaagtg catcttcatg
     2821 gtctggtgtg atgatctcaa tacttgcatc ttcctcgatt ggacgagtca aatcaacgag
     2881 ttttccgttc actttaccag ctaaagcttt ttttgctaag ctttttgaaa tgctttcagc
     2941 aattgccaat gttgttgttc cagactcaaa ttctttgact gcaccatctg gaaaagtaat
     3001 ttttatcata atagtttcct ccttgaaatg ttttgattga tcagatcatc aagctgaaaa
     3061 acaaaaaaat cctagcatct cttgaaaaga gatactagga cgaaaagttc gtggttccac
     3121 ctaatgttca aacgcaaatg cgttcctcaa gcgttgtaac ggtccgaccg cccttgcttc
     3181 aacaagggtt tcgaaagtgg tcatcctttg gttttctcct ggaaaagttt cagcctagct
     3241 tttcctctct tatcgaagac tctcaaattc agttggcttt ctcatcaatc aattatgtgt
     3301 ttatttaacc actaataaat agaaaaatca agtgggaaac taaacttatt ttttccattc
     3361 cccatttaca ccgtatgttc cttttttcat ctcattgatg atgacatgga tgtgttcttt
     3421 tggtgcctgc gtgtttttat gaacagcttc cgtgatatct ttcatcatat tttctaattg
     3481 ttccggactg cgaccttcta ctaattcgac atgtacgaat ggcataatgc tctctcctct
     3541 ttctgatcgt tcttttttca actcgattct atcatactat gtttgtcttg ggtactgaat
     3601 caggaaaaat ggctggatct ttttttgctc tcaaggcact caaaatagag accgtatcca
     3661 cgacttcttg caaaacagct ccgaataatg cggggattag tcccgtactt gcaattaaca
     3721 tcaaaaaaat acaaataaag atgccaatca gtacagactg tttcgctatt ctcatcgtat
     3781 ctcttgaaat cactactgct tttgctacac gcaagagatc atctttcaag atgaccgcat
     3841 ctgcactttc actagcagct gtagaaccat gagcccccat tgcaataccg atatctgcag
     3901 cagcaagtgc tggtgcgtca ttcacgccgt ctcctaccat cgcaacaggt ctttgatctt
     3961 tagggatact ttctaaaatg gtcagtttat cttgaggcaa gctacgggca tgtacagaat
     4021 ctaattggac tttttctgca atcgtttgag caatggcaga tccatccccg gtgatcatca
     4081 tcaaattttt cacatcaagt tttcttagct ccttgattgt ctttgctgct tctgggcgaa
     4141 tctgatccga aaataggatc gttcctgcaa aagatcgatc aattgttaca tagattgctg
     4201 tattggacgt ttctatttct tcagctgaaa caaaagaggc acgtccaatc tttacttcat
     4261 gattattgac tactgcttgg attccttgac cggtcacttc tttcagttga ctaacaggat
     4321 acagttggct gtttttcacg ttgttcacta acgaacgagc taagatatgg ctagattcct
     4381 gttctgcact agctgcatac agaagtagct cttctttttt tattccattg actggctgaa
     4441 tcgcctctac ttccaatatc cctttggtaa gcgttcctgt tttatcaaaa gcaatcgttt
     4501 ttgtacgtgc gagcttttca atcgttgttc cagtttttac aacgatcccg tttttacttg
     4561 aacggctcat tcctgcaact aaagcaatgg gcgcagctag aatcagtgga caaggtgatg
     4621 ccactaccag tacctcagca aatcgtaaag ggtcttttga gaaccaccaa gaaataccac
     4681 cgatcaaata agcaatcaac gtgaaaggga ctgcataacg atcagctaaa cggacaaatc
     4741 gggcaggctt ttcttttgat tcttcgacta gtttaataat catttgatac tggctgtctg
     4801 cggcacgttt ttcagcgatc atttggatcg atccgtctcc atttaaagac cctgacataa
     4861 tctgatctcc aggtttcttg gtttccgggc gggactcacc tgtcaaagaa gattcatcga
     4921 ccgaagattc tcctttggtc actcgaccat ctgcgggtac aagttcgcca ggcttgacta
     4981 gtaactggtc atcaatattt atttcatcga ttactatatc ctgcaatttt ccttctttga
     5041 atcgatgggc aattcttggg gtgttatcta acaaagactg cagttcttta cccgctcgtt
     5101 tattcgcgta atcctctaga ctatctccgc ccgtaagcat gatcaatatc atcaaactag
     5161 cccagtattc tttgacggct aatgttgcaa ggattgcggt aatcgctaat acatctacgc
     5221 catatcttcc tgaacgtaac gtcttgatca tttccaaaaa catcaagaag gcgagaatcc
     5281 ctccagtaaa tgcgacaatc gcaaatgcaa gggccggctg gtgaaaaaca aattcactta
     5341 ataaagctat cccaccaata agcaaagtaa gtaaaaattt tttgaaatgt gtcattgttt
     5401 tttctcctct tttatgtgac ttagagaggt tagtaataaa tttccagctg cctctttcat
     5461 cttaagcata gtccttgact agcacttttt aaaacaatag gccttaattg aaaacaacta
     5521 tcactatcag cgtttccaaa attcatcagt tctacaaaca aaaatattca gcaaggatta
     5581 ctttttcgat tcatcagtta aaaagtacct tttttcgaaa aactatgcta tacttttaat
     5641 tcgttattta cggcggaagg agcaaacatg cgatgaatga gaaacaacta gaacaaacgc
     5701 acgtggatga aacgatcgaa ctaatccaga tcgaacaaaa tatgctggat acaaaacaac
     5761 aagaattgac tcaggaaatg aatcattcga caaaagacac agctaatcac aagattcgag
     5821 ctggttccaa tgaagctttt tatgaatctg ctgttgaata tcgccaacat gagcaggaat
     5881 tgcttttgaa ataccatact cttgaagcac agcaaaaacg cctgcaaacg ttgtctgtca
     5941 tgaaagatag tccttatttt gcccgtatcg actttaaaga agaccgtgaa acagaaacat
     6001 tatatctagg tatcgcttct ttaagagatc tggcagaaga aacaatcgtt attgactggc
     6061 gcgcaccaat tgccaatctt tattatgaag gtgagattgg tcccgcattt tatgaaactg
     6121 aaacagatag aatagatgtc gatctgcagt tgaaacgaca atttaaaatc caagaaggac
     6181 gtattttatc tatggtagat acgtcggaag taatcaatga tgactttttg ctagagatat
     6241 tggatgaatc atcaagcaac catatgaaaa acatcgtctc tacgatacaa aaagcacaaa
     6301 atgcaatcat tcgggacaca acaagtaaag taatgctgat tgaaggaatc gccggaagtg
     6361 gaaagacatc cgctttgctt caacgtattg cttttctgtt gtatcataat cgcaagtggc
     6421 tcgaggctga aaacgttctt ctattttcac ccaaccattt gttttctgat tacatctcga
     6481 ctgtattgcc ttcattaggt gaaagcggcg tgcctacaca gacattcaaa aattatttgg
     6541 tgcagctgct gccagagttt tctttactag atgaacagca acaagaaagt ggttttttgc
     6601 taggggaaaa agatccgata caagtgatga aaagcggatt gaccttagtc gatcagattg
     6661 atcgctatat ccaaggcatc acaaactacg gtcccttgtt ccgtgatatg aaaataaatg
     6721 gccgtacgat cctatcaaag gaatcgattc gccaatggta taaagaaaca aatgaacaat
     6781 tacctttaca tcatagactt tctctattac aaacgaagct cctgaaaaaa ttaggcggtc
     6841 tgcaaaaaga cgaaacacgt cagcaatggg tcaaagatct ggcggaagaa cagctccaag
     6901 aactatacgc aacagatcca aatcttgaat atacagaaca aaaggagcgt tcattacgta
     6961 aaaaactggc caaccaaatc gtacgtaaaa gattccgtcg aatccatcga gggatcatga
     7021 attatcaatt tgtcaatata acgaaacaat atctccactt tctacaaact gtgccacttt
     7081 ctcttctaac acgacataaa atcagcgaga ccaggtggca ggaacatttg acaactgttc
     7141 gtacacactt gcgtcaaaga gaattgcgtc aagaggatgc tgtactattc tttttactga
     7201 tgcgccgaac atatccagtg acggttacac acaaagctcg ctatatcttt attgatgaaa
     7261 tgcaggattt cgcaccatca caagtggcat tgctgcaagc cttgttccca aaagcaaatt
     7321 tgactttctg tggagatcta aatcaaaatg tctttggaaa tgaaacgatc gttggatcat
     7381 tggaagacct tttcgtggac cgtgaagtca ctcgtttcca attaacgaca agctatcgct
     7441 ccacaaaaga aatcactgat tttgctaatc attttattgt gaaagaacat caagtagaaa
     7501 caacagcaag aaaaggagac cttcctttgc tcatccaagg aacaacagat gatatcaaat
     7561 tgaattggtt agcagatacc attcgaacga ctcagggaaa agcccggtat tggcgtacag
     7621 caatcatagc taaaacacaa gaagaatgcg aagctcttta tgctcaactg ccagacgagt
     7681 tgaaacaaca agtccagctg attttaaatg agagtgactt tatgaagcgt tccattattt
     7741 tacttccagc ctttttagca aaaggtctgg agtttgaccg agttttcttg tgggatgtag
     7801 atgcagaaca tttttcttct cagcaagaca aattgatcct ttacaccatg tgtacacgtg
     7861 cgatgcatga attgaccctt tttacctcca ataatgaaag tcctttgtta aacaatatgg
     7921 atatttcatc ttatgagaaa aaagtactgt aagtaaaaag acaactgcta tcattaatgt
     7981 tagcagttgt ctttttattt acaataataa ttgcagaaaa agtaattcaa tcatttaagg
     8041 tcatttattc tatatataaa tccaaatctg tttcaggata ttttaatttg acaagcgaaa
     8101 taatcatttt cagttcttcc atgttttctg gatattctgg gtcaaatacc ttgctatgac
     8161 ggccaatccc gccattgtcg atcaatcggt aaattcgatt ccattcaatg aattggtcaa
     8221 ttgattggaa attgctgatt ggatagccgc cacaatccaa aatctctgat acacgaaatg
     8281 ctaggtatgc actatcgatc actttatttt ttttcgtgtc tatcttctca tttctttcga
     8341 taagaatttt ttcaggacga tgtttcaagc tccactctaa tagtagaaag aaagcctcca
     8401 tccgatactc ttcttcaaat aaccgaaagg ctgaatcaga acgacgaaaa agcagcggaa
     8461 tcagataatt ttgtctataa atcagcattg gtgcgcggtc caccatacac tctaaataac
     8521 ggttcagata ccctgtaccg acatagtttc ctaatcgata gccttcaacg ccgaatcttg
     8581 cgtatttttg cgctaatttt gaacaatcag ttacgttcac agctaactct tctactttgc
     8641 ggtaaccgct taagatacgt tcaaaaggtt ctaaagtgat ttttgcttcc atattcttcc
     8701 ccgtccatct tccacatact tcatatcatt tcacatgata cttatgctct catcatacca
     8761 caggttgaat tagaaaagaa aataaatcac acaaaaaagc aagacaatct gttagtgaga
     8821 ttgtcttgct tttcgaaaaa gactgaatac ttttcgttaa aactggttgc ttagtcttct
     8881 aatgccacat tatggaacac atgctgtaca tcttctagat cttccagtgc atcaatcatt
     8941 ttttcaaatg tttcttgatc ttctggagat agtgttacct cgttttgagg gatcatttgg
     9001 atctcagcga ttgaaaaatc agtgattcct ttttctttta atgcttcttg tatactatgg
     9061 aaatccgcta cttcaccgta aacgatgatt tgtccttctt cttccattac atcgcgaaca
     9121 tcgatgtctt tttccattag gtattcaagg atctcgtcag cgtcttctcc tgcaaaagca
     9181 aagattgctg tattgtcaaa catgtaagaa accgctccgg aaacacccat gttcccgcca
     9241 tttttcccaa atgctgcccg tacgtctgct gctgttcgat tcacattgtt cgtcaatgta
     9301 tcaacaatca ccattgagcc gcttggtcca aatccttcat agcgcaattc agagtatgtt
     9361 tcatctcctg caccttttgc tttttcgatt gctcggtcaa taatatgttt tggtacatta
     9421 taagttttag cgcgatcaat gacgaaacgt agtttttggt ttgcatgcgg atcaggatca
     9481 cctgattttg ctgccgcgta gatttcaatc ccaaattttg cataaatccg gctattatta
     9541 gcatctttag cagttttctt tgctacgata tttgcccatt tacgtcccat attttcactt
     9601 cgctttctga tttgatagtt tcataacgca aaaacgtcct gttccattat acttgtgaat
     9661 cacgattctg tgaagctttt tttctttctt ccttcattct tctcattttt agtcaattat
     9721 ctatactcgg attgggattt gcccgaaatt ctgttccgga atattgcctc ccaattgata
     9781 gatttgatca cttctaacta acagttccgc ttcttgtgcc tctttttttc cgattcggga
     9841 ataaatagaa atctcttttt tttcttttaa gaatcttctt ccagcatcgg taaatccaag
     9901 tagtcgaaga taatttttat tttgttggac aggaatctct tgctttttga tattcagcaa
     9961 gacataacaa gataatcgtt ggatccgtgt ccatgtatat ctttttgttt ttacctttga
    10021 cacaaaatcc tcaaaactat cagcagttgc agctgctgtt ttcatgcgcg attctattcc
    10081 ttcggtcatc tgatagatat ctgtcagttc cagcaaagta gaagataaca accgataacg
    10141 caaatacggc caataagatt cccaagtctg cagcgaacaa gttgaaagat cttcttctgt
    10201 ctgaggcggc acaagtccag ctattctttt tccttcttct aacgcatggc gaatggctgt
    10261 tgcactggct atcctctgat ctgtgatgat tttttcatga tagcctgcac ttttacgtcg
    10321 gatcggatgt aattccatag ggtggacata cttggcattt tcctttgcat aacttaatcc
    10381 caagatatga tttggcgaag aaaaatcaag cgtaatatcg ggatacagac tgcgaaaaac
    10441 ctcagtcatt ttttgcggat aactatacgt ttgatcagac agttcttgaa atgcctcttc
    10501 tatcctatct tgattctctt ggacaaagcg tccgaaactg ctgtaatcaa acgaagtctc
    10561 ttcttctgtt ccaaaacaca aactgtcaca gtccagtgtt tgcaagattt ttactgcacc
    10621 tttagcaaaa tagtcagctg actgaaccgc ccattcgacg ggaagctcca ctactaaatc
    10681 cacaccattt tgtaaagctt cattcgcgcg atgccattta tcgatgatag ccggttcgcc
    10741 tctttgcaaa aaattcccgc tcattacagc aatgacgacc tctgctcctg ttatttttcg
    10801 tgccatttcc acatgatagc gatgtccatt gtgaaaagga ttgtactcta cgataatccc
    10861 gcagcttttc atgcgcatcc tccttcatag ttatcgataa aaaagaaata gagacaagta
    10921 aaaaaaggca aaaatcggaa aaaatacccg aactatataa actattctcg caaacacaat
    10981 acttctgcga cgaaaagctg cactgcaaga gcaatgccta tatcgttcgg atatataatt
    11041 tatgttgaag atttattctt ttcttgttaa ctcgagcagg caaaaccgct agtctttgta
    11101 tgctacaaag aaccagcgct ttgatttctc tgatgctgct tcgtctgtaa aatcagaaaa
    11161 tgcttcgact ttcgaaaagc cgctgttttc cagcattctt aaataacttt ccaatggata
    11221 ggtacgctct tgatgtagtt catcttcacg aataaatcga ccagaccctt cttgttcttc
    11281 aacgaaaaag gtaagaaaat gttcgatgct gtgtttctcg cttcctggat aactatccca
    11341 caaaaaagca aaattatccc cttgataatg atagctatat tctggaaaaa cctcatctac
    11401 ttgataggtg gagtgtacgt caaataaaaa tcggccattt tcttctaacg cttgatagac
    11461 ttcatcaaac acttgctgta cctcttgtct atttttcata tagcacagcg aatcagaaaa
    11521 acaggtaatt gcctgatatt gtccgatttc tgataaatcg agcatattgc cttccacaaa
    11581 ttggatcgat gcgtcttctt catatgctcg ctggctggca atcatcaaca tctcttctga
    11641 aaggtctaac cctgtcactt tatatccatc ttttgcaaag gcaaccgcta atgctccagt
    11701 accgcatgcc agttccaaaa tattttttgt gtcttcttgt aaatgacgtt tcgaaaaagc
    11761 taaccattgc tgataaagtg tttcgtccat cacttcatcg taaatgaaag caaatgtttc
    11821 ataagccata tgttaatcca accattcact aagatcgact agcggagcat ctgaccatag
    11881 tttttccaga ttatagaaat ttcgttctgg cgcatggaaa acatgtacga tcacatctcc
    11941 aagatcgatc aatacccatt ttccgccttc ttttccttca atacgtttta cttcatattt
    12001 gttttcttct tctttatcaa tgatttcttc tgtaattgcg ttgatttgac gttcgctatt
    12061 tgctgaacag atcatgaaat aatctgccaa caaggaaaca tttcgaacgt ctaaagcgat
    12121 aatgtcttct gcccgttttg aatctgctgc ttcaatcgca atttttaatt gttctttact
    12181 atctatgatg atttcctcct aatatgagca gcttattttt ttgctaccca gtaattatat
    12241 gtttcgattg tttttggata aatctgttgt tcttgcgcaa gcaaatgggc caatgtatgt
    12301 tttgtttcaa atgctacagc ttcgtctaga tcagcccatg caatggctcg tgcatcttgt
    12361 accccaggaa aatctcgacc tggttcaata tagtctgcta cataaatgat tttgtcaagg
    12421 agactcatct tagcagcccc agtcgtatgc acccggattg catgaagaat ctctgcatcg
    12481 gtgattccta attcccgctc tacaaacgaa gcgccaacaa gaccatgcca gattgcatta
    12541 ttataattca ataactccgg atcaaaaccg tctcttacga ttaccatttt gaactcctca
    12601 tctggtcgtt cttttgcata atcatgtgtc aatgcagcaa tactagcttt ttcaggagaa
    12661 gcaccgtatt ttttggcaag agccacggct gtttcttcta ctccaagcac atgcttgaag
    12721 cgttgttcgc tcattctcat ctgtattttt tgcatcaggt tttctctaga ataagcagta
    12781 tattcattac tatagtccat attcatacaa cccttcattt tgaatataat caattacttt
    12841 atctggaaca aggtagcgga tagaacagcc ttctttgatc ttttgacgta ttttcgtgga
    12901 actgatgtcg atctcaggta catctaccca aattacagga tatggagtat ccgttgtgta
    12961 tcctggtcga cgtataccca caaagttgac catagaagtc agctcatcga ttttatacca
    13021 ttttggcaaa tattctacca tatccccacc gataataaag taataatctg tatccggatt
    13081 gttttgcgtc aattccttca tagtatcata ggtgtaactt ttcccaccac gagctaattc
    13141 aatcgtttca atctgcaaaa aaggattgtc ttccaccgct aattccaaca tgttgagtcg
    13201 atgttttgca tcaatcgtct gtttctcatc aacatgaggt ggctgataag aaggcatcaa
    13261 aaatacgcgg tcaagaccta aattccggcc agcttgttca gccatcacta aatgtgcaag
    13321 gtgaacggga ttgaatgttc cgcccaagat ccctacttgc tttttcttct ctaagaattg
    13381 aggcatttct tcaggaaata attgtgaacg aacaaatgtc ttagcttgcg tattcatcat
    13441 ggtcactcct tatattgctt ttacttcttt tgatagcctt tgatactttt cttttgtgga
    13501 aggtttgtat aatacaataa cacgtccaat gatttggacg atttcgcaat ctatttcttt
    13561 ttctaatact tgagctgctt cttctgcaat ctcatctgta ttttgaagta acgatacttt
    13621 gatcagttct cttttttcta aagcttcttc aatttgaacg atcattgctg aattgatccc
    13681 accttttccg atttgaaaaa tcggctgaag atgatgcgct ttgctacgta aaaaacgctt
    13741 ttgttttcct cttaaactca taattttcct tcttccttaa atcaatgctt tgcgtttcaa
    13801 tacatctaca ccctttggcg cccagcccgc aattactact ggcgctgtta cagtgatcca
    13861 gcctagaccg gcaaaaacga tgtctgtttt ttctttcaca gaaaactcaa aacgaacaag
    13921 ctccggaaat gcctcgactt catcaggacg aggaggctgt aataatccac ctacatgttt
    13981 ttgataaaat tcgtctgctt tttccagttt cgttcgatga atatttacat catttgacac
    14041 ataagcaata aatgaagcac gattgccgct gataaaatca aatcttgcaa gtccgcccaa
    14101 gaaaagtgtt tgcccttcat tcagctgata aactttaggt ttgatttctt ttaacggtgc
    14161 aatgatccgc aaatcttttt tacctaagta gtgcgccatt tgatggcgat ggataattcc
    14221 cggtgtatca atcaaaaaat gcccgtcatc taaaggaatc tcgattttat ccaaggtcgt
    14281 tcctggaaac tgcgaagttg tgattacatc ttgtactccc gctgtatttt tgatgatttg
    14341 attgataagc gtcgattttc cgacatttgt tactccgacg atatagacat cccgtccatc
    14401 gcggtattct tcgatttttt ctaatagttc cgtcatctca tgagatttct tagcactagt
    14461 taataataca tctactggac gaattcctgc ttcgtaagca cgttccttca tccattgtgt
    14521 taatttagtt tttttcaatg atttaggtaa tatatccact ttgttcccaa ctaaaagtac
    14581 tggattgtca ccgacaaaac ggtgtagtcc tgggattagt gaaccattga agtcaaaaat
    14641 atccaccaca ttgacgatca atgcatcttt ttgccctaat tcattcagta agcgcaaaaa
    14701 gtcgtcatcc gtcaatgaga catcttgggt ctcattataa tggcgtaatc tgaaacaacg
    14761 ctgacaatag acttccccgg aagctaaacc tttttccaaa gccgtttgcg gcgtgtatcc
    14821 taattcaccg ggtttgtttg tttgtataac ggcaccacag ccgatacatt gtaattcttc
    14881 tgtcattcga ttccacctcg ccagcccata tcagggtgtt ttgctaatag ataggccata
    14941 attctttttt cccaaaaacg attaaattgt gtcttccaac tatctgttgc gataatgggt
    15001 tttacaagga tgctacgtat ccctgcacga ttggctccac gtatatcagt cataatctga
    15061 tcgcctacca ttactatctc atcatctgct aaatccaatt gtttctttgc tatgttgatc
    15121 cctcttgcta aaggtttcat tgctcgtgca acatacaaaa gatcaaattt ttcaatcgct
    15181 cgcgcgacac gttttgaatt attgtttgaa acaacaacaa cagggattcc tgcatttttc
    15241 atttctaaaa tccaagtaag caactcttct gttccatctg gattattcca tgcaatcaac
    15301 gtattgtcta aatcggtcaa tactgctttg atgcctagat tctttaattg ggcaggtgta
    15361 atctgataga tggcttctac catccatgtt ggtttgtaat ttgaaaacat gctgtctcct
    15421 catctgcaaa aaatgaagga gcacataaag tactcctcaa ccccgtatta tactgcatat
    15481 cagcaaaaaa aactacctct atactcgttc gtctttcaga aaaagcattt ctttacaaaa
    15541 tgaccaaaaa taaagattga tcacagaaaa atttagaaac aaaaaacaac taatattggc
    15601 ggaacctctt gtatcgtttt tccagtaatt tatgctgatc taatcgaaca agttccgtca
    15661 atctttcaat caacgctttt ttcataatgt gtgcgacttt atatcgtggc aactgctcgc
    15721 cgttgaacgt ttcaggaata actcgatcaa cgattcctaa ctcgcgtagt tctggcgcag
    15781 taattttcat caactcagct gcttctgcgg cgcgactacc gtctttccac aaaatcgacg
    15841 caaatccttc tggagaaagc acagcataaa tcgtttcttc cagcatccag acttcatccg
    15901 ctaaacctag agcaagggca ccgccacttc caccttcacc aatgatgatg gcgatgatcg
    15961 gtacctttaa atcagacatt tcgagcagat ttctggcaat agcctctcct tcgccgcgct
    16021 cttctgctcc tacaccgcaa tatgcacctg ctgtattgat caaggtaatc accggtctat
    16081 tgaacttttc cgcttgtttc atcagtcgta aagcttttcg atagccttca ggatggggag
    16141 aaccaaaatt tctgcggata ttttcctgca gatcccgtcc tttttgaata ccgatcactg
    16201 ttaccggttt gccatctaat cgagcgattc caccaacaat cgcttcgtca tctgcaaata
    16261 aacgatcacc atggcattcc tcgaaagaat cgaagatctc ttcaaaaaaa tccaagcttg
    16321 tccatcgttc tttagctctt gctaactgaa cgacatcatg tgctgtctta tccacgatct
    16381 atccatcctt tctgtgtatg caattcaaga agcgtatgga ttttctgttt caattccatg
    16441 cggggaacaa tctgatcgac aaaaccatga gataataaga attcagcctt ttggaaatct
    16501 tctggaagtt cctgcttgat cgtctgttcg atgacgcgtc ttccagcaaa accgatcaag
    16561 ctttggggtt ctgctagaat aatatcgcct tccattgcaa aactggcagt tactcctccg
    16621 gtcgtcgggt ccgttaaaac agttagataa aaaagacctt cattgctatg ccgtttgaca
    16681 gcagcagaga tttttgccat ctgcattaga gaaaagattc cttcttgcat ccgtgcgcca
    16741 cctgaagctg tgaacaagac gactggaaga ctttcctttg ttgctcgttc gaataatctg
    16801 gtgatctttt ctccgacaat cgttcccata cttcccatga taaaattagg atccatcact
    16861 ccaataatga agggaatccc ctgaatggtt gctttaccag tgaggactgc ttcatgaagt
    16921 cctgttttat cttgcatttt tttaattttt tccgtatatt cgggaaatgc tagcggatct
    16981 ttggttacaa gatccgtatc ccattcttca aaactttttt catcaataat caaagctaga
    17041 cgctgccatg caccgattcg aaaagcgtat ccgcaatgag ggcatacttt ttcttcaccg
    17101 atgtctttcg tataaaggat atgtttgcaa tttggacact ttgcccacat gttatcagga
    17161 acagatggcg agttagcagg tttgctctct ctattaggat tgatccgaat atatttcttt
    17221 ttcttaaaca gagccatcta tgcgcctcct atctccgttt ctggtgtcca attcggtaaa
    17281 aattcttctt gtaaaaatgc tgtgctgtaa tcacctgcta tgactgcagg atgactaatc
    17341 aaatccatct gaaattctgc gttcgtgatg attccttctg tgataaattc acttaaggct
    17401 cgctgcatct tcattaatgc ttcaaaacgc gtatttccat ggacgattat ttttgcaatc
    17461 attgaatcat aatatggcgg gatcgtatat cccgggtaaa ctgcactgtc tactcgcagt
    17521 cccatcccac ctgctggaag cagtaagttt tggatcttcc ctggagaagg agcaaaatgg
    17581 aaagcaggat tttctgcatt aatccggcat tcgattgcgt gtccttggaa tactacttct
    17641 tcttgacaca cattcaaagg ctcaccggct gctatctcca cctgtttttt tactaaatcg
    17701 attcctgtga ccatttctgt tactggatgt tccacttgga ttcgagtatt catttccatg
    17761 aaataaaaat cgccttcttc atccatcaga aactcgatcg ttccggcatt ttcataattg
    17821 actgctttag ctgctcgaac agcagcgtct cctagtgctt ctctttttgt ttgtgagatc
    17881 actacagaag gagattcttc cagtactttt tgattgttgc gttgtaaaga acaatctcgc
    17941 tcgcctaaat gaatgacatg accaaatcga tcacctaaga tctgaacttc tatatggcga
    18001 gcaggataaa tgattttttc caaatacata tcatcatttc caaatgccgc tgctgcttct
    18061 tgctgagctg aagaaaaatg ctgtgggagc tcttcttttg attgaacttt ccgaatgcct
    18121 ttcccgccac cacctgcagc tgctttcagc atcactggat aaccaattct gtctgctatt
    18181 tccaatgctt cttctactgt agataataca ccagtacttc ctggaatgac tgggacattt
    18241 gcttcttgca tcaattgacg cgcattgatc ttatttccca ttgcatcgat cgttgcactt
    18301 ttaggaccaa taaatgtgat attgcactct tcacacatgg aggcaaatcg gctattttca
    18361 gataaaaaac caaatcctgg atgaattgct tcggcttttg tcacgattgc agcgctcagt
    18421 acttcctgaa cattcagata tgaatcaacg gcttttgccg ggccaatgca gattgcttca
    18481 tctgctaact gtgtatgaag tgcttcggca tctgcttctg aatatatcgc tacggtttgg
    18541 atccctagtt cgcggcatgc acgaatgata cgtacagcga tttcaccgcg gttagctatc
    18601 aatactttag aaaacatatt tcacctatcc aatcataaag gttaattccg cttcggctac
    18661 ttttttaccg tctactttag cgattccttt gcctattccg gctgaagctt tcactttcaa
    18721 tatctcaact tcaaggtata aggtatctcc cggtgtaact ttcttacgga attttgcttt
    18781 gtccaatccg ccgaaataag ccgtctttcc cttgaattgt tcaagagaaa gtaatgcaac
    18841 tgctcctgct tgagccattg cttcaacaat caatacccct ggcataacag gttcttctgg
    18901 aaaatgtcct tgaaaaaagg gctcattgat cgttacattt ttcttagcaa cgatccgttc
    18961 tccttcaacc atttcttcca ctcgatcaat caaaagcatt ggataacgat gtggaatgat
    19021 ttcttttatt tcttgtatgt tcattgtttt ccctctttcc tgctttgctt atttttgctg
    19081 aatcctaaag agtggctgtc catattcgac aactgactcg ttttctacaa ggatttctgt
    19141 gatcactccg tcggcctcac tagttatctc attcatcagt ttcatcgctt caataatgca
    19201 gataacttct cctttggcaa cttgatcacc tacttttttg aaagctggtt tgtcgggcgc
    19261 tggttgcaga taaacgattc ccacgattgg cgaaacaacc tcgattccat caaatactgc
    19321 cgattctttt tctttcatta tttctggagc agttgttgct tgttcaggta tgacattgac
    19381 tggctccgaa actggcaacg tttcgtttgt ttgaaccata ttcatgcctc gacttgtttt
    19441 atttttattc atatacaatt caaattgacc ttcacgcaaa tcgaattccg ttagcgagga
    19501 ttgatcaaat tgactgacta attctctgat ttcattgata tccaaaatta ttcctcccag
    19561 cgtttcatgc acagaaccgc attatgtccg ccaaacccta atgaattact cattgcataa
    19621 gtaaaacttg caggtttgct ttcgtttttg acaactgtca agtcaatctc aggatcttgt
    19681 ttttctacat tgatcgtagg tgggataaat tgatgttcta atgccagcaa agttgccaag
    19741 gcttcgatcc cgccggctgc tcccaataaa tgtccagtca tcgatttcgt gctgctgacg
    19801 taagtatctc tgaaattttc acctaatgcg atttggattg cttttgcttc tgcctggtca
    19861 ttggctggtg tacttgttcc gtgagcattg atatatccga cttgttccgg ctgtacacca
    19921 gcttcttcca tcgcaagctt cattgcttta gatgcaccag aaccatctgg agtaggagct
    19981 gtcatatggt aagcgtcaca gtttgcaccg tatccaacga tttctccata gattttggca
    20041 ccgcgagctt gtgcatgttc taaagattca agaactagaa cgcctgctcc ttcacccatc
    20101 acaaaaccat tccgattttc gtcaaacgga atcgaaccac gattaggatc agttgattct
    20161 gagagtgctg ttaaagctgc aaatccagca atccccatct cacaaatcgt tgcttctgtt
    20221 cctccagcaa gaatgacatc agaatagcca tgtttgatgt tacggaatgc ttccccaatt
    20281 gaattcgttc cgctagcaca agctgttacg atagctgtac aaataccttt tgcacctaca
    20341 cgcaaagcaa tatttccagc tgccatgttg gcaatcgcca ttggtacgaa cattggagaa
    20401 actcgttttg cacctttttc atgtagacga ataacttggt tttcgattgt tggaagacca
    20461 ccgatcccag atccgatgat gacgccaaac cggtcttgat tcattttttc tttatctaaa
    20521 ccgctcatct ccattgcttc taaagcagcg tagattccat agatcgaaaa catatccatt
    20581 cgtttgctat cttttttaat aaaatattta tcgtaaggga aatctttgac ttcacccgct
    20641 aaggtaatcc ctgtttctga agcatcaaat ttagtgatcg gtgcgatgcc attcgtcccc
    20701 tcttttaagc tattcaaaaa gctgtctgcg tcatttccaa taggtgaagt gactccatag
    20761 ccagtaatta ccactcgatt catttgtttg ctctcctttt cttatccgtg catgaccaat
    20821 ccgccatcga cattgatgac ttgtccggtg atatatggat tttgtgctaa gaaaactgct
    20881 gtttgtgcaa tatcttctac ttgtccgaaa cgctgcatcg gaatttgctt ttccgcttgt
    20941 tccttgactt tgtctgctaa aacttccgtc atatcagttg tgatgaatcc tggcgcaatg
    21001 gcattacatg taatgcctct agttgcagct tctcgtgcca cagattttgt taatcctact
    21061 acgccagctt tactagctgc ataatttgct tgaccaatat ttccaatcaa cccagataca
    21121 ctagagagat taatgatggc cccttctcgc tgcttcatca tttttttcaa tacgtgctgc
    21181 gtcatattga aggttccaat caaattgata tccagacatt ttttgaaatc ttctgcatcc
    21241 attcgcatca caagtttgtc atttgtgatt ccggcattgt ttactaacac atggatcgat
    21301 ccaagctttt cttctgcttc tttgatcatt tgtcccgctt tctcataatc agagatgtca
    21361 ccggaaactc ctacgcattt tacaccgaat gcttcgattt cttctatctt ttcttttgga
    21421 atctctccgc gaccattcaa aatgatattt gctccagctt ttgcaaaagc taaggccatc
    21481 gctttgccga ttcctcttgt actacctgtg ataaatacat tttttccttt tacatccata
    21541 acgttcctcc ttatagtatt gctattgttt catcaaatgt ctgcttattc tcaacacgca
    21601 gaactggtat tgttttatcg atttttttca taaatccgct taatactttt ccggggccga
    21661 cttcgatgac ttgttcgatg ccgattgttt ttaacttatg gatactctca tagaaacgaa
    21721 caggttgcat gacttgctgt tcaagtagtc ccgcgattgt ttctttcttc attatttccg
    21781 ttgttgtgtt gcttatgata ggaaaagaag gttcagagaa ttgaatctga cttaggtcct
    21841 tcgccagttt ttttgctgcc ggttctaaaa tagctgtatg aaaaggcccg ctgacattta
    21901 gagggatcat tcttttgaac cctttttctt ttaatagtaa aacagcttcg tctactgctt
    21961 tttcttcgcc accaatcacg atctgctgag gtgtattata atttgcagga gatacgatgc
    22021 cgtacttgct agcttcatga cagctttcct cgatcgtttc aatcggcgca ttcatcactg
    22081 caaccatttt cccgcttcca gctggggcag cttctgtcat ataagcgcct ctttttgcta
    22141 ctaacgcaac tgcttcagtg aaggatagcg cattactagc tactaatgcc gaatattctc
    22201 ctaaactcag tcctaaagct gcatctggga tgattccatg ctccttcaat aagcgataat
    22261 acgcgatgct gactgttaga atagctggct gtgtatattg cgtctgatcc aaacgttcat
    22321 tttctgtaaa gcaaagttca gccatatcgt agccgagaat ctcacttgct tcatcaaatg
    22381 tctgctttac aacggcttct tcatataaat cttttcccat accttgatat tgtgcacctt
    22441 ggccactaaa taagaatgct gttttcatgt ttttctccaa tatatccaga atatatgttt
    22501 gattcttcct attcaaaaag gccgagacat tcctatttca ggaaatgtcc cagcccccaa
    22561 cctaatttta atgattatgc agtttgtttt tctacgtaag tcactaaatc ttgaaccgtt
    22621 ttgattccgt cttctgtttc gattttcacg tcaaattcat cttcaatttc attgatgatt
    22681 tggaataaat ccaagctgtc agcttctaag tcttcttgga tgtcagtagt caatttgatt
    22741 tcttcagttt cttttcctaa ttcttctgcg atgatttctt gaattttttc gaataccata
    22801 ataatatatt cctccgatat tgtttgcttt ttatttttat aatttaacag tctacaactc
    22861 catgagaata cttccccatg ttaaacctcc gccatagcct gtaaatatta ctttctgctg
    22921 ttttcctaag gtgatctttc cactagaaac tgcttcatcc aataagatag ggatgcttgc
    22981 agcagatgta ttcccatatt tatccatatt ggtcaggaac ttttcttgcg gcatctttac
    23041 ttttcgggca attttctcaa taattctgag gtttgcctga tgcaaaagca gataatcaac
    23101 tggtttgtct ttcatgactt cttggatatt gtttgcaacg tctcgtacgg caaaatcaaa
    23161 aatatctctg ccagtcattt gcaaataata cgaaccttct gaatcttctg aatagaacgg
    23221 atttttattt tgctggtagc cagcggtcaa tgagctactt ctggtaccgt cagctgctaa
    23281 tttttctttc agcaattttt tctgtggatt cgcttctaag agaatcccac ctgctccatc
    23341 gccaaacaac acagcagtcg tacgatcaga ccaatcgatt agcttagata gtgtttcacc
    23401 accaatcacc agtcccttag ttgagccgca gcggatcatt ttttcagcca tgcttaacgc
    23461 ataaacaaat cctgagcacg cagcactgat atcaaaagcg atagcatgag ttgctcccaa
    23521 tttcccttgt acttgtgctg ctacagatgg tgtcgtgtaa tcgggcgaca ttgttgctac
    23581 taaaataaaa tccaaatctt tagggtttag atttttctgt tttatcaatt tttccgccac
    23641 taaaatgcat aaatcagaag tctcctgatt tgttacacat cttcgttcac gaatacccgt
    23701 ccttgacgaa atccattcat cagaggtatc catcatcttg gaaagatcat tattagtaat
    23761 aatcttttca ggaaggacgc tggctgttgc agtaatcatt ccaaattgtt ccattaaata
    23821 tcctcactta tagtcttgca agaagtcgtg caagtttttc agtgcatcca gcaaggcatg
    23881 ctgttcctcc gtagccattc cgtctaagat gttcttgacc atttcacgat gaaaatgctg
    23941 atggacccga aatagtaact ttcctttttt cgttagtccc aactttacaa cgcggcggtc
    24001 atcttcactg cggatacgtt cgacatagcc tttttttact aacttgttaa tagcagtggt
    24061 caaggtccca acagtaattg atagttcttt ggctacttct gaagttgttt ttttcttgta
    24121 catcccgatt gcctcgatcg tgtgcatctc tgtgattgac agatctttga attgagattt
    24181 tttcagctca gactcttcga ttgtcaaaat atcattgaag acactaacaa gataatcatt
    24241 tactgttttt aaatctggtt ccatgttcca ccgtccacta attagtttga ttatcaaatc
    24301 ttttgatgtt caaactatat cttaactttt ttcaaaatgc aaccttaaaa gatcattttt
    24361 ttactatatg agcttttttt aagattttac tcatttaaaa caactgataa atcagcattt
    24421 ataatttctt ttattagaaa aatcatctat ctcacatatt ttgattatca aagtattttc
    24481 tgtgaacaga actatagaat tccctaaaac ttgtccatta tttcaaaatt tgttatagtc
    24541 tttttattca aagcgtaaaa ggagatccta tgttaaaaga aaaaatgaaa actctcccca
    24601 tgctgccggg tgtctatctt atgaaagacc gagatggtaa ggtcatctat gttggtaaag
    24661 caaaacggct caaaaataga gtaagcagct attttcatca gaataaacag cattcaaaaa
    24721 aagtattacg tatgattcat catataacag attttgattt cgtcgtagta gacacggaat
    24781 tagatgcttt attgctcgag tgtcaattga tccaacatta ccgtcctttt tataaccgac
    24841 aaatgaatta tttcagtaat tataattatg tacatattac caataaggga tttgtattaa
    24901 cagatactcc tacggctcga acatacgggc cttttcgtct ctataaaaaa atgccgtcta
    24961 ttttgcgtat aatggaagaa acttatcaaa tgccttggtt gtctgaaatt agtttgttag
    25021 ctttgcgagt ccagcttcct gacctacagg aaatgacttt tgaacagaaa aaaaaggaac
    25081 tccaagggct gtttcaaggc cgaaataaaa agctactgac ttacttgaaa aaaagacaac
    25141 aacatttcat ctatcagttg aatttcgaaa aagccggtat gcttcaaaaa gatatcgaat
    25201 tagttactta ttttattaga cggatccagg aacaaaagca gtttttaaga acaccatcgc
    25261 tcaccttcag catgccatta gcagcagatg agagtcagaa aaagcactac ctcgtttgtt
    25321 atggtcaact tgcagaaaca atgattacct ctggtgacgt ttctcctgac ttctattatg
    25381 aaaagaaaga agctcattta tctttgaaac gtcaattgtc aaaagaagaa atcgatcctg
    25441 tacaaatctt gatcagttac cgcaaaaaat tagaaaaaga acagtttgaa attgaactgt
    25501 taaacaaaaa agaagctgaa aagcaactaa attag
//
LOCUS       DARBDV010000035        25196 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000035 DARBDV010000000
VERSION     DARBDV010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.035"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            12..84
                     /locus_tag="SCM56_001339"
     tRNA            12..84
                     /locus_tag="SCM56_001339"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="SCM56_001340"
     tRNA            88..160
                     /locus_tag="SCM56_001340"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="SCM56_001341"
     tRNA            168..249
                     /locus_tag="SCM56_001341"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="SCM56_001342"
     tRNA            267..339
                     /locus_tag="SCM56_001342"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="SCM56_001343"
     tRNA            351..422
                     /locus_tag="SCM56_001343"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="SCM56_001344"
     tRNA            439..524
                     /locus_tag="SCM56_001344"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="SCM56_001345"
     tRNA            535..608
                     /locus_tag="SCM56_001345"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="SCM56_001346"
     tRNA            618..691
                     /locus_tag="SCM56_001346"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="SCM56_001347"
     tRNA            711..783
                     /locus_tag="SCM56_001347"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="SCM56_001348"
     tRNA            811..884
                     /locus_tag="SCM56_001348"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="SCM56_001349"
     tRNA            898..971
                     /locus_tag="SCM56_001349"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="SCM56_001350"
     tRNA            995..1084
                     /locus_tag="SCM56_001350"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="SCM56_001351"
     tRNA            1097..1170
                     /locus_tag="SCM56_001351"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="SCM56_001352"
     tRNA            1189..1264
                     /locus_tag="SCM56_001352"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="SCM56_001353"
     tRNA            1266..1336
                     /locus_tag="SCM56_001353"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="SCM56_001354"
     tRNA            1350..1423
                     /locus_tag="SCM56_001354"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="SCM56_001355"
     tRNA            1448..1535
                     /locus_tag="SCM56_001355"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="SCM56_001356"
     tRNA            1557..1628
                     /locus_tag="SCM56_001356"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="SCM56_001357"
     tRNA            1651..1740
                     /locus_tag="SCM56_001357"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="SCM56_001358"
     tRNA            1753..1826
                     /locus_tag="SCM56_001358"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="SCM56_001359"
     tRNA            1845..1917
                     /locus_tag="SCM56_001359"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="SCM56_001360"
     tRNA            1921..1993
                     /locus_tag="SCM56_001360"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="SCM56_001361"
     tRNA            1998..2068
                     /locus_tag="SCM56_001361"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="SCM56_001362"
     tRNA            2081..2154
                     /locus_tag="SCM56_001362"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="SCM56_001363"
     tRNA            2166..2237
                     /locus_tag="SCM56_001363"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /locus_tag="SCM56_001364"
     CDS             2503..3696
                     /locus_tag="SCM56_001364"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEG1110150.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="SCM56_001365"
     CDS             3826..5298
                     /locus_tag="SCM56_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1110151.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="SCM56_001366"
     CDS             5381..6169
                     /locus_tag="SCM56_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEG1110152.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="SCM56_001367"
     CDS             complement(6236..6904)
                     /locus_tag="SCM56_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEG1110153.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="SCM56_001368"
     CDS             7019..8731
                     /locus_tag="SCM56_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1110154.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10503
                     /locus_tag="SCM56_001369"
     CDS             8734..10503
                     /locus_tag="SCM56_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1110155.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(10708..11274)
                     /locus_tag="SCM56_001370"
     CDS             complement(10708..11274)
                     /locus_tag="SCM56_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEG1110156.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(11274..12230)
                     /locus_tag="SCM56_001371"
     CDS             complement(11274..12230)
                     /locus_tag="SCM56_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEG1110157.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(12230..12886)
                     /locus_tag="SCM56_001372"
     CDS             complement(12230..12886)
                     /locus_tag="SCM56_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEG1110158.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            13256..15274
                     /locus_tag="SCM56_001373"
     CDS             13256..15274
                     /locus_tag="SCM56_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG1110159.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    15344..15550
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15701..18115
                     /locus_tag="SCM56_001374"
     CDS             15701..18115
                     /locus_tag="SCM56_001374"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEG1110160.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            18463..20016
                     /locus_tag="SCM56_001375"
     CDS             18463..20016
                     /locus_tag="SCM56_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEG1110161.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(20397..22181)
                     /locus_tag="SCM56_001376"
     CDS             complement(20397..22181)
                     /locus_tag="SCM56_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1110162.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(22196..23917)
                     /locus_tag="SCM56_001377"
     CDS             complement(22196..23917)
                     /locus_tag="SCM56_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1110163.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            24017..24778
                     /locus_tag="SCM56_001378"
     CDS             24017..24778
                     /locus_tag="SCM56_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEG1110164.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         7795 a         4629 c         5364 g         7408 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 taaagaggtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag
    10561 cttctcatgt ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt
    10621 gaataaagag gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac
    10681 atatcattta tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg
    10741 cggctggttc ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag
    10801 tttggaacaa aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta
    10861 gacgacaagg atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt
    10921 gcgtgtagtc tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt
    10981 gaaaatccct gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc
    11041 ttgtagaaac aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc
    11101 atcgattgct tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc
    11161 tgctagaaaa accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg
    11221 ttgcaagatt tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg
    11281 ctcacctttt tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca
    11341 tcaattgcat tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc
    11401 cacggcgcat cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg
    11461 tcgcaaatga cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag
    11521 tcccgcaaca ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata
    11581 tctgaatcta aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt
    11641 aatccgttga tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca
    11701 tctaaataag tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt
    11761 gtttgcaatc caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca
    11821 actacttcat ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt
    11881 aaattgacta cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta
    11941 aaattttgga aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg
    12001 atttctttct ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa
    12061 ccagatacac tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag
    12121 tcaaatcctc catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg
    12181 taattccaag aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct
    12241 tctttctgta agagttatct atttgttttc ttttttctct atacactgga taagtcaggc
    12301 acagccaaga aagactgagt aagtaaccgc cgatgatatc agtaggaaaa tgaacgccca
    12361 gataaatgcg gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg
    12421 tgcgtaaggt tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa
    12481 tcatactgcc tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg
    12541 ccaatgaagg acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa
    12601 ttacaataaa tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc
    12661 ataaagccgc acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa
    12721 agagttggta gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat
    12781 cgaaaccaga aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga
    12841 caagaaagaa gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt
    12901 gaagcaatga cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac
    12961 caatgtatga ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga
    13021 aagacatttt ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa
    13081 tccttttaaa caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt
    13141 acggtaaatt acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat
    13201 attaagagaa gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga
    13261 aaacattgca agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa
    13321 acgaggggca gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt
    13381 acagccggtt cctgttcaag cggatgaaac acctgttcag ttcggcgcaa gaatcaatac
    13441 agcggcattt atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct
    13501 atatgcatca gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt
    13561 atctcaagca ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt
    13621 ctacatggat acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg
    13681 acaatatcct tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc
    13741 atttggcaac ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga
    13801 tgcgacagct tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa
    13861 taatatcatc gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg
    13921 cggtggagcg actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag
    13981 caattctggt aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg
    14041 tgattctgtt tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg
    14101 gaacaacatc caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca
    14161 ggaaaatggc tcttcgacga ataattccgg taacaatacc aattcttcag gaaatgctgg
    14221 tacatcaaac ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt
    14281 ttggaagatc tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat
    14341 caagaacaat ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa
    14401 tgctggagca aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc
    14461 aagttcttct aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg
    14521 gagcgtctca aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca
    14581 aaataacttt atctacccag gacaacaact gactgtttca aacagtggat caacaaacag
    14641 cacgtcaggt aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg
    14701 cagcagttcg tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc
    14761 aaataaaaac ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt
    14821 catttatcca ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg
    14881 ttcaactact tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc
    14941 atccactggt aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa
    15001 caaacatcat atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat
    15061 ctatccagga cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc
    15121 agttactggt tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga
    15181 cagtcatgga attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat
    15241 ttatccaggt caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt
    15301 gaattccaca aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg
    15361 aagtagtaaa aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc
    15421 ttttgaactc gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg
    15481 gccgttacaa cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta
    15541 cgtcctataa aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag
    15601 aaaacaaagc aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat
    15661 aatagaaaca agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga
    15721 aattgagaaa aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga
    15781 tcctgaaaag ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct
    15841 acatgtaggg catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag
    15901 tcaaggatac aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca
    15961 atatgcgcta gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt
    16021 ccgacgccaa atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac
    16081 tgatccggag tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct
    16141 agcttatgaa gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa
    16201 tgaagaagtg atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat
    16261 gcgccaatgg atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt
    16321 agttgattgg ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg
    16381 agcaaatatt gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg
    16441 accagatact ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag
    16501 agaaattacg acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa
    16561 aaaatcagat ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc
    16621 ttatgcaatc aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt
    16681 atcttcatac ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga
    16741 attcgctcaa actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa
    16801 agctgcttac acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa
    16861 acaagaagca atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga
    16921 agtcagctat cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat
    16981 cccaatcatc cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt
    17041 acggttgcct aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa
    17101 tatcgaggag tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac
    17161 gaatacaatg ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca
    17221 taacaaaaaa gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta
    17281 cattggtgga gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt
    17341 cttatacgat atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg
    17401 aatgatctta ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc
    17461 tgatgatgtg gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg
    17521 gccattagat gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt
    17581 ggatcgtgta tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac
    17641 attgaatgat ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga
    17701 ctatgaaaat cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc
    17761 ctataaagtg gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc
    17821 gattgctcct cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc
    17881 ttacgcacca tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt
    17941 tttccaagta aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga
    18001 tgagctagaa aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac
    18061 agtcagaaaa gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc
    18121 tctttgtcaa taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca
    18181 ttctgtctca gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga
    18241 ttttcatgtt ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct
    18301 ctgtaatcat caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga
    18361 aattgagagt acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga
    18421 ttttgacgca taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt
    18481 attatcgaag tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa
    18541 agaataatgt tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct
    18601 gtatcgttcc tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa
    18661 ttaaataatt tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt
    18721 gtttttcttc tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg
    18781 cttagtcaaa gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat
    18841 ctttattttg agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag
    18901 ttattgctga gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc
    18961 ggaatgaaca cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag
    19021 tcttcgcctg gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc
    19081 attcttttag ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat
    19141 ataaatgctg cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca
    19201 aatgaagggt tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat
    19261 gatctcaaac ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat
    19321 tgggtatttg tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg
    19381 gggctgattc ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca
    19441 tggtttatct attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa
    19501 acatggcaaa cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta
    19561 ttttatggca acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact
    19621 ttgtacattt acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg
    19681 atcaatcagc cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca
    19741 cgtgaaagtt tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg
    19801 attccggaaa actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact
    19861 caaataagcg cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat
    19921 gcagggaaaa tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc
    19981 gttcagcaac tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg
    20041 ttcagcaccg agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt
    20101 gtaactcgtg attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc
    20161 gtattttaca agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt
    20221 aaaaagggat tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc
    20281 caaaaagtat cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa
    20341 aagctatcga tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac
    20401 gctatctgaa attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca
    20461 gaatgtgttc ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt
    20521 tcaatcgttg acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc
    20581 attgcttgct gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa
    20641 ataacgacag gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct
    20701 tgagataacg tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga
    20761 ataaagtgat cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc
    20821 attttcccgt aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa
    20881 accatcccaa aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg
    20941 aattcgatct ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt
    21001 tttcctgcac cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg
    21061 acttgattca tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt
    21121 tttcctttcg gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca
    21181 tctaatatct caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca
    21241 taagaagcgg tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag
    21301 aagccgatcg tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg
    21361 aaggcgagtt gattaatgaa acgaatggcc ggatagatag cacagttcat aaattgagca
    21421 cgtttaaaag cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct
    21481 tgctgattga atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt
    21541 aaaacaccca gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta
    21601 gttgtaacaa ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg
    21661 atataaaaca gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg
    21721 atacctgtca ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg
    21781 tcacctactt gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt
    21841 tcttttcgca ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata
    21901 aatgaagtga tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat
    21961 gcttgtttga tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag
    22021 agatcatcga tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat
    22081 aaactgcata gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa
    22141 cgtatcaaag cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata
    22201 ttccttgtga ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag
    22261 taccttgtcc aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg
    22321 tactgaggcg ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg
    22381 ctcttaatgc tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga
    22441 tctctgctgg tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat
    22501 aattagaacc gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt
    22561 cttctaattg agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc
    22621 ccatctgcaa gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac
    22681 tgatgtggga gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta
    22741 cttttcctat cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag
    22801 atccggtccc gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt
    22861 ctagaactgg atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat
    22921 ttgtctgatc aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct
    22981 ctgcttgcag tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca
    23041 ataccatagc tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag
    23101 tcacgacacc gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag
    23161 aagcataaac gattcccatg agcaaaaaag caattggagt gacccaagca aatattcggt
    23221 tcactttgat catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat
    23281 atgattgatt atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga
    23341 tggtgatccg atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca
    23401 gtcgataaac aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata
    23461 gagaataacg aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca
    23521 tttggcaaaa taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca
    23581 aaagagatcc agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga
    23641 aatatttctc ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag
    23701 aggcaactgc agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt
    23761 ttataactga ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg
    23821 gtacacctaa tgtaccggcg atttctaaac agatcatcac aaaggtaagg agagcgagtc
    23881 ctttgttttt ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat
    23941 acttttcgat tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa
    24001 aaagaggtga gaaaaaatga aaaaaatgta tacgattggc caagtatcgg aactatttga
    24061 tttgccaaaa tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca
    24121 agaagagaat gattatcgat tgtttgatat cgatgatatt tttatgcttt atgatatcgt
    24181 tttttataga aaattagata taccgatcaa acagatgaag aatttatatg gaaaaacatt
    24241 gacggagtta tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt
    24301 gatgaaacaa aaacaaaaag agataaggga gcaaaaaaaa cagttgaaat tgatgatcga
    24361 tacaaacgag gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga
    24421 gtttgaagat attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat
    24481 gagtatgcca gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt
    24541 acatttattt actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt
    24601 cttattgaaa tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag
    24661 aatgtatgaa aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac
    24721 ggatgaaaac aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga
    24781 aatatgtgtt taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct
    24841 ggttcgatta cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt
    24901 tttagaggaa atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg
    24961 taattgtcct cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat
    25021 ggtgaactga gactaatgag aagaaaaatt catattgtta tgagtgagat tgtatcagat
    25081 gagaatgata aattaaatag gtgcataaaa gcattacact taaaatacat tgttgcttac
    25141 attattacaa tatgttaata aggcaaaaat aaatagatgg attgaataaa catatt
//
LOCUS       DARBDV010000036        24575 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000036 DARBDV010000000
VERSION     DARBDV010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.036"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(152..550)
                     /locus_tag="SCM56_001379"
     CDS             complement(152..550)
                     /locus_tag="SCM56_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEG1110165.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(547..1320)
                     /locus_tag="SCM56_001380"
     CDS             complement(547..1320)
                     /locus_tag="SCM56_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEG1110166.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1317..2000)
                     /locus_tag="SCM56_001381"
     CDS             complement(1317..2000)
                     /locus_tag="SCM56_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEG1110167.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2167..3054)
                     /locus_tag="SCM56_001382"
     CDS             complement(2167..3054)
                     /locus_tag="SCM56_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1110168.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3051..3920)
                     /locus_tag="SCM56_001383"
     CDS             complement(3051..3920)
                     /locus_tag="SCM56_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1110169.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4057..5343)
                     /locus_tag="SCM56_001384"
     CDS             complement(4057..5343)
                     /locus_tag="SCM56_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG1110170.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5616..6194
                     /locus_tag="SCM56_001385"
     CDS             5616..6194
                     /locus_tag="SCM56_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEG1110171.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPMKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6194..7123
                     /locus_tag="SCM56_001386"
     CDS             6194..7123
                     /locus_tag="SCM56_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1110172.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7120..8856
                     /locus_tag="SCM56_001387"
     CDS             7120..8856
                     /locus_tag="SCM56_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEG1110173.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="SCM56_001388"
     CDS             complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="SCM56_001388"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEG1110174.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9752..10261)
                     /locus_tag="SCM56_001389"
     CDS             complement(9752..10261)
                     /locus_tag="SCM56_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEG1110175.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="SCM56_001390"
     CDS             complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="SCM56_001390"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEG1110176.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11562..12653)
                     /locus_tag="SCM56_001391"
     CDS             complement(11562..12653)
                     /locus_tag="SCM56_001391"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEG1110177.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="SCM56_001392"
     CDS             complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="SCM56_001392"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEG1110178.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="SCM56_001393"
     CDS             complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="SCM56_001393"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEG1110179.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="SCM56_001394"
     CDS             complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="SCM56_001394"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEG1110180.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16616..17590)
                     /locus_tag="SCM56_001395"
     CDS             complement(16616..17590)
                     /locus_tag="SCM56_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HEG1110181.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17571..19454)
                     /locus_tag="SCM56_001396"
     CDS             complement(17571..19454)
                     /locus_tag="SCM56_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HEG1110182.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19472..21697)
                     /locus_tag="SCM56_001397"
     CDS             complement(19472..21697)
                     /locus_tag="SCM56_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEG1110183.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21812..22846
                     /locus_tag="SCM56_001398"
     CDS             21812..22846
                     /locus_tag="SCM56_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1110184.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22913..23926)
                     /locus_tag="SCM56_001399"
     CDS             complement(22913..23926)
                     /locus_tag="SCM56_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG1110185.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYWGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24004..24192)
                     /locus_tag="SCM56_001400"
     CDS             complement(24004..24192)
                     /locus_tag="SCM56_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEG1110186.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         7061 a         5341 c         4397 g         7776 t
ORIGIN      
        1 gcatattttg aaaagaaagt ctgatgctta gagtagaatt tcataaaaat ttactatttc
       61 ataagtattc acgagaaaaa tgcaaacaaa aaagcggaaa taacgctcat ccgccttctt
      121 gtttgtgttt atttcctctt caaacgcttg tctatgattc agacttttct ctatcgttat
      181 tttcatctaa aagttcttgc attccgataa tttctcgata ttgatgaatc ttagtgatac
      241 caatgacttt gtctacatca atactaaaac aaataccatt acctggaata tcgatgttga
      301 tgccttgacc aatcgcatcc attactttat tcgtcaaact atcgggtact aatgtcaaca
      361 caatatcttt ttctggcgaa atgtccaaac ctaaaaattt ccctgaatca tgtacaccag
      421 aacctcttcc atgtaagatc gttccgcctg ctgcacctgc ttcttttgaa aagtcgataa
      481 catcttcgcc cattcctccg tcaacgatcg tcacaatcat gtgaagattc aatgctacaa
      541 gatctttcat ttgctttccc ccaatttttg caatctttct ttttcgattc tgtctttttc
      601 atcttttcgt ttataaatat atcctagtac taaagtggat aatattggtg tcaatgcaat
      661 cattgcaacc acaccaaacc cgtcaatgat cggatctctt ccttcaattt gtgcagcgat
      721 tgccacgctc attgataaaa taaatgtcga acacatcggg cccgttgcta ctccaccgtt
      781 atcaaaagcc attgctaaaa agatcttgtc cacttttcgt cctaaaataa atgctagccc
      841 gtatcctggg atcaagaaat aataaagaga gaatccgacg agcaaacgcc acatagacaa
      901 tcctactgct gcgccgatcc caattgaaat taccatcagc atgattttcg atcggattct
      961 cccctcgctc aattcttcaa cctgtttgat cattacatga atcgccggct cagcaaaccc
     1021 tactaagaac cccattaaaa agccaagggg aaccagtata taattgtaag gcaatcccgc
     1081 aatagcactg cctaaatgct gtcctaccgg tacataagcc atattgactc cgtgcaggaa
     1141 aaggatcaac ccaatagtcg ttatcaagaa acctttcata atgtcaaaga aacgcttttt
     1201 gcgcatacga aaactaatga tattcattaa aacaaatagg attacgatag gtgccactgc
     1261 caaaatgact tccatggtaa cgatttcgat tccatctatc acttgctgcc agaaactcat
     1321 tggaagatca cccccaaaat catcacggca agaatcggcc ctaacgaagc aataccgacc
     1381 attccaaaac tgtcattttc atttttcttt tgtttgatca tactagtcat ccctccggca
     1441 agggccaaga taaatggaac agttactgga ccagtagtca ctccccccga atcaaaagca
     1501 atcggcataa attcggaact ggtgaaaaat gaggcgatca aaacgcctat gtagcctata
     1561 gccatcaatt gataatagga tagcttgaat acgacccgaa gcagcgcaaa ggctagaaat
     1621 actccagtgc cgacgctgac gatcccaatc agcaacactc tgccaatctc tccgtcagat
     1681 atatcatata cttgttgtcc caaaacttgc acggaaggtt cagcaatcgt gatcacgatc
     1741 ccgatagcaa atcccaaact gaccaaaacg gtcaaacttt tctttttgat catatatttc
     1801 cctactagat ctccgacttc catcatagaa taatctgctc caaacaaaaa gagcgtcatt
     1861 ccgatcatca tgattgccgc accaaccaga aatgaagcga ggtcttctcc ttctaacggt
     1921 gcaaaaacaa acgttaaaat gacaatcagg acagtcatcg gcaatatagc agcaatcact
     1981 tctttaaaat tttttagcat gtatcctcct cctttactct ttaagtttac cttaaaatga
     2041 aagagttttc gagcacaaac ttaaaacgtt atcatttaat ttttctcaac ataaaataac
     2101 acacgtccca aaagtatctt tttacttgga aaaatgactt tgaaggaacg cgcttattgt
     2161 gtttttttat tttacagagc cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa
     2221 tacgatggca gttggtaaag tagcgatgac taatccagcc atgatcattc catagtctgg
     2281 tgtataggaa gcacccatag cagataaaac aagcggaacc gtccgtttat caggagattg
     2341 caaagctact aagggccata aataattgtt ccagctactc atgaatgtga tgattgcagc
     2401 cgcggcatag gtatttttag ccgtcggcat atacactcta aaaaaaatac ttagttcttt
     2461 tagaccgtct aatcgagcgg cttctactag atcttttgga aaagatttaa cattttgtcg
     2521 aaagaagaag atcaaaaaag cagtgctaac agatggcagg atgacaacca agaaactgtt
     2581 gattcccaaa accgttccgt tcaattgtga aaacattcga tataaaggga tcatcaatgc
     2641 cgcaaaagga accatcatcg atagcagtag aatattgaaa attaggtctt ttcgtttact
     2701 ttgataaatc tcaaatccat atcctgccat tgacgaaatc agcaatgcaa acacagtggt
     2761 caccaaagcg atcatcgctg aattccaaag ggaacgagta aaattcagct cattggaaaa
     2821 caaattttga aaattgatga tcaagttact gccaatcttt atttttcccg atgtgatatc
     2881 cacgggtgta ttcgtcattc ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga
     2941 taggatgctt aatgctgtat actttaatac tgacccaatt gtcgaacgaa gttctaatgc
     3001 ttgttttttg acgttggggc gttcgattgt ttctttttct accatatttt tcatcgactt
     3061 tctcctccta ctttcgtttg gattatcgac aatatcacaa taaaggaaac aataacaaag
     3121 gaaactgctg ccgcatatcc aaaattaggc gtgaatttat aactcaaatt atagatgtat
     3181 tgagataggg tggtcgttgc atttgctggt cccccgccag taatattttg tacttcatca
     3241 aaaagctgca atgtcccgat cgtcgaagta atggaagtaa acaaaatgat tggttttaaa
     3301 tttgggatcg tcactttgat aaactgctgc catttagaag caccgtcaat agatgctgct
     3361 tcataaatct ccgaatcaat attttgcatg cctgaaagga aaaaaatcat attgtatcca
     3421 gtccaacgcc aagtgatcga aagaatgatc agaactctcg cccaaattgg atgagtcaac
     3481 cagccaatcg gtgtgtcaat cataccaatg cccaacaaca agttgtttac aaggccagat
     3541 tgagaaaata gacttttcat gatcagagaa taactgacca tcgaagtgat acatggaaga
     3601 aagatagcgg ttcgaaacaa ccctttatat ttcaactttt tatcatttaa aaggttggaa
     3661 ataaaaagtg ctagaaacaa catgatcggt acttgtacaa gtaaatacag gaatgtatta
     3721 aaaaaagctt ttttaaaagt tgcatctgtc aacatccgct gataattacc taacccgtta
     3781 aatgtcatat tgacaggggg accactttga aaagacgtga tcaaagccaa aaccatcgga
     3841 ataaaaacca tgattgatat caaaatgact ggcaagatta aaaaagcatg cccgtttttg
     3901 aataaatctt tctgcttcat tcttttccct ccaattctat cattgattat ctacatgaaa
     3961 agacaagaaa gacaatatct gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg
     4021 atgaaaaagc gaagaaattc acttttttac caccgatcaa ttagccaatt gtgcttctac
     4081 ttgtttttgt gtatctgcta gtaccttgtc tgtctcttct ccattgatga ttcgatgaag
     4141 agcttcagct acgactgctt caattgcata ggtctcatgt ccgtaattca cttctggtat
     4201 ttcagatgtc catgttgaaa aatcgtcaaa gatcttttga ccactgtaaa attcagaagc
     4261 ttcttgataa gcttcttgtt cattcgcgct aagcatcgta gatactagtc cgatttcttt
     4321 cgcaagagtg cccatcagtt cttcattcga agcaaacgtt gcttctagga aatctttcgc
     4381 tgaatcttct ccagctactc ctttgatcac ataccagcca gcgccgccaa ggttagaagc
     4441 ttgcgctttt tgcagatttt ctggaagtgc aggtattggt gcaatcttcc attttccaga
     4501 ttggttttct gctccttgga tcgttgaaga ataccaagcg ccagttgggc tagaagctac
     4561 tgctcctgat tgtatcgcag ttactccagc attccagtca gaggtttgtt cgactagatc
     4621 ttctttcaaa agagtggcaa acaattcaag accatacttt aaagatgcat tgtcttggat
     4681 cgtgactgtc tccccatctt cagatgtata ccattctcct gcctgttgca taatcatcct
     4741 tactcgacca aggtcagaag gattgacttc agcgattttc tttcccgtct tttctttgac
     4801 atctcgcgct acttgaatat actcgtccca cttcagattc tccatatcct cttctgtata
     4861 acctgcttct gccatcagat ccgtacgata gaagtttgct gttacgcccg aatcgaaagg
     4921 aacaccgtat attttatctc ctactttgtt taccgcaaat ttatacggtg caaaatgatc
     4981 ttcctttaca atagcagaca aattagcgaa tgcatctgga taagaagtca aatagccttg
     5041 tatcctataa tcttcaatca agacgatatt gggcaatcct tctgtattcc cgctcgctaa
     5101 agcggtgttc agtttctgaa cgatatcatc ctgagacatc gtaacaactt ctatctctgt
     5161 ttcttcgttc tcataaactt tttttgcttc attcactgct ttgatattga atgtctcatc
     5221 ccacgcccag acagtgactt tttcttcttt gtctgctgta ctactagcac ttccgcatcc
     5281 tgttaagaaa cctgatgctg ccgctaaaca aacgactcca gcaattaact ttctgatgtt
     5341 catttttatc cgctcctttt ccatccatta gttgtttacg tttgctacat tcatcatacc
     5401 aaaatttgaa aacggtttct ttattattca gatacttcaa aaatttctcc taaaataata
     5461 aaaaaataca aatcaactat gacaaaagga ataatcttag catctagacc aaagatcgaa
     5521 atggtgaggt ttctttaaaa taaaaatatc cttgcatttt ctcctagaaa taagtatttt
     5581 taaattacaa gactattctt aaggcgggta aaaaaatgcc aatacgcgta attcttatcg
     5641 atgatgaaca gacgatttta gatggaatga aacagttgat caattggtct gagcacgggt
     5701 ttcaattaat agggactttt tccagtccga tgaaagcgct tgatttttct ttggtacatg
     5761 caatcgatat cgtggtcaca gatgtaatga tgcctgaatt aaatggaatc gaattatcgc
     5821 ggctattaaa acagcaacaa cctgaaacac agatccttat cctctctagc tatgatgaat
     5881 ttgatatggt taaagattct tttaaagaag gcgtcgctga ttatctttta aagcctcgat
     5941 tgagtcctga tttttttcta aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc
     6001 ccaacaaaag cccaatcacc aatcgttatg agcagatctg cgagaatctt tcacagcaga
     6061 ttgctggaca aactcgtcca ttgcttgacc ctcttctttt tgaacacacc ttgtttttct
     6121 gttatatacc gaaaaacggc cattcagttc aaaacaaggc tctaaacaaa aacagtcgat
     6181 cacccattca ttgatggata gcgacgattc gtttactatt tatcctgttt ctaccttttc
     6241 ggaagaatcc ggctatgtaa tcaatactga ttccaaagat acacttagga agtttgttca
     6301 aaagtttcgt caagaagttt ttccaggtga tttttttgtt gtttctgatt cttttgcaat
     6361 ccaagatatc aatgaaatat tccaagagat cagaaattgc accaaaggac aacgtttcta
     6421 tcataaggga aagaaccttg cttatcagca tgaacttctt ccgttattaa ccaaccacga
     6481 gcagcaagca agtagttatt taacaaaaat cgttcataaa gactacgctt tagctgtcaa
     6541 agaacttgag aatttcttca cgacgatatt ggaacagcct attgatccag cttatttgaa
     6601 acatgaggca atcaatcgtt tctatgcatt actcaacgct ttggctgacg agcatgttgg
     6661 gaatgaagaa ctttctttgc tcaagatcgc ccttccttct ttattagccg atgctgagta
     6721 tttcgaagat ttttctattc ttgtcaaaga aacacttgag aaactcaccc acttttctgc
     6781 aaatgcaaaa aaagacaatt tcgatatctt gactttgatc tacgagttcg tccaggaaaa
     6841 ttatgagaaa gaaatcaatt tacaactttt atccgaaaaa tatcattttt catatagcta
     6901 tctctcagcc atctttactg aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt
     6961 ccgtatcagt aaagccaagc tctttttgac ggaaacacgg cttactttga cggaaatctg
     7021 tgagaaagtc ggctataccg aactgggtta tttctccaga gtttttaaag aagagacagg
     7081 aatgacacct tctcaatacc gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag
     7141 catgaactct ttgccaaact tctgttcatt ttgttcatcg gtctttttct gcaagccatc
     7201 gtcatttctt tgtttattta tcatcgatca agggatgcct atatccagct gttcaatcaa
     7261 tcgaatgatg tggtactaaa aaagattcaa tctgaatttg aaagtttgaa tgatacaatc
     7321 gagaatacat tagccgcttt tgattctaat ccagctgtca aaagctattt ttccaatgat
     7381 ccagcccaac atatggaaca gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct
     7441 ctttcaaaaa tccatccaat gatcgattac gatgtattga tctttggaga aaatggaaga
     7501 acttttgtcg gaaacgacat gctcactgcc gtatctgccg attctttttt tcaatctgcg
     7561 attgctcaga gagtgaatga acgtgctgct gatacacaaa tgctttttgc acaccacgga
     7621 ttgactcttc gcgataagaa agcacccagc gtcttctttg tcaaaaaact gaaaaataca
     7681 ttgaatcacg tttacggata tgctgtttta tcgatctcat ccaagcaact agcgaatctt
     7741 tttcaatctg tagtaaatcc tgagatatcg aagatctcct tcatgaacga agaacagacg
     7801 atcatcgcat caaatgaaga aaatacaatt gggagccgat caatgatttt caaaaaacta
     7861 acagtaggtg aaacaacgat ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc
     7921 caggattgta cattactcag tcaagtcgat cttcattctt tatctaacca aatgggcgtg
     7981 atttttccga ttattttgta taatatcgtg acacttgctt ttgctggctc attggtattt
     8041 atgtatttgg atcgacacac aaaatcgatc taccgcttga ttgattcgct aaaacggcta
     8101 gaggaagatg gcttaaaagc tactgttcct gtacaaggaa gccatgaagt ccaattgcta
     8161 gggaatacga tcaatcaatt gctaagtaat gctcagcatc attatgaaca gacgatccaa
     8221 gacgaaaaga agaaacgttc gcttgaaatc caaactatgc aagcacagat acagccccat
     8281 tttatttata atacgctgac tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa
     8341 gcagttcaag gaatcgacag tttcattact ttactaagaa gtacgattgg gaaaaaagaa
     8401 gaagtgattc cagtcaagga agaaatcaaa agcgtacaaa gttacatcga tcttttgagt
     8461 atgcgatatg gcgaaagaat ctcggctaaa ataatgattc ctgatgaact gcagtcatta
     8521 tctatgccga atatgatcct tcaaccaatt atcgaaaacg cctatcttca tgcttttcac
     8581 aataaaacgc aaggattcat cacggtgtac ggcaaaacaa aaaatcagct tcttttattc
     8641 gaagtgattg ataacggcga tggcttcgat actgaaaata ccaaacaaaa aaatgatcat
     8701 ttttcaggaa tcggcatcag ccatgtagat gagcggatac gcctaatgta tggcgatgca
     8761 tatggcttaa cgatccaatc agtgattggg acaggaacga ctgtaacgat ttgtctaccg
     8821 cttcttccta aaaatcaatc agaatatcaa aaataaagaa tagccaagcg ctgatttttt
     8881 tattttaagt taaataaatc aatcagtatt cttgactttc atctacgcgt ctcttctact
     8941 gtcagcactg gataacagcc caatatttga ctttctcctc cgagcatctt gatttcttgg
     9001 agcgcattct ctactaatgt cattggacgg tccaacaaca aatcgatcac gaaaaaatat
     9061 tctcccaagc tagttttcaa tggtcgggat tcaatcttac ttaagttgat ttcccgccag
     9121 ccaaaagctg ccagcatttt atgaagcatc cctggccgat tagcaggtag tgtcaagatg
     9181 acactcattt ttactggagc tgactgctgg ctggtcatct ttcggtcacc gacgatccaa
     9241 aaacgtgtct ggttcaactc attatcttgg atattctcag ctaagatctc caatccaaca
     9301 tgctgtgctg tttctaatga agcaatcgct gctgcgtctt ctttcggatg ttctgctaca
     9361 tacattgctg cagcagttgt tgattctgtt gctactaaag gaacattagg ataatgcgtt
     9421 tctaaaaact gttgtgattg tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt
     9481 gttgctggat tgcctaaaag ctgctgcttg ataggaagaa cgatttcact tttaacttcg
     9541 acttctggat gtttggaaag aagatcgatt gtgtgatgaa cagctccttc caatgaattc
     9601 tctacaggaa cgacagccag atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg
     9661 atggaagcat acgcagtcaa ttgttctgta ggaaatagct gacaagccgc ctgataagtg
     9721 aatgaacttt ctggccctag atagctgacg ttcatatgct tttgatcctt tctataatgg
     9781 tatcgatgat cttttctggt gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt
     9841 cataccaaga aagtcgttgt gccaataatg cttttatttc ttctcttgtt tttccaagag
     9901 ctaaaggtcg aatagacgtt tcatcttgtt ggatacgatc aactagacag tccgcttctg
     9961 cctctaagta aagaacaatc ggctgtgttt ttaaaaaacg ccgattttct gcactttgga
    10021 caatccctcc accagtcgcg ataattttgt cttcatttga cagctttctt aaaatatctg
    10081 actccacttt tcgaaagctt ttttcaccgt aatagtgaaa atattcggca atcgacaatt
    10141 ggatatgttc ctcaatcttt gtatctaaat ctacatactc tttttgtaaa cattgagcta
    10201 accctttacc gattgttgtt tttccagcgc ccatgaaacc aatcagcaaa atgctagcca
    10261 tgagcttctc ctcctgacac aagttctgct aaatcgtcaa aaaatgccgg ataagagatt
    10321 gaaacagctt cagctccttc tagttcgcaa ggtccatcag ctatcaaagc agctacttgc
    10381 agcatcatac cgattcgatg atcgcctctg ctggacactt ttgcgccatg cagaggtgtc
    10441 ggcccatgga tgatcagacc gtctgctgtc gcttttatct ttgctcccat tttctgtaat
    10501 tcttctgcca ccgcatcaat tctgttggtt tcttttactt ttaattcttc tgcgtctttg
    10561 atgatcgtga ttccttctgc ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc
    10621 agacgcggaa tgatttctcc accaatcgtt gctttcttca aatgactcgt cttgacactt
    10681 aaatcagccg attggttctg ttcattgtga ttcgtctggg taatcgctgc gcccatctcc
    10741 tccaacacat ctaagatacc agttcttgtt ggattgaccc cgacattttt taacaacagt
    10801 tggctttccg gaacaagaag tcctgctgct agaaagaaag cagctgatga aatatcacct
    10861 ggaacagtta tctgttgacc agttaatttt tgcggtccgg tcactatgat cgttttatct
    10921 tccactgtga tccttccgcc aaattgacga atcatttctt ccgtatggtt acgggaacgt
    10981 tctttttcaa cgattacgga tgtgccttct gcttgtaatg ccgcaaacag caaagcagat
    11041 ttgacttgcg cactggctac aggcatattg tatcggatag gtgataaaga gcgggttcct
    11101 gtcacagtga ttggtagata ctggtcattt tcttctcctt gcaagtcagc gcccatctcg
    11161 cgtaaaggtt ccatcactcg ccccattgga cgtttagaca acgaagcatc cccgaacaga
    11221 gtagtcgtaa aaggctgtcc agccaaaata cccatcaata aacgtatggt cgtaccggag
    11281 tttcccatgt caattgattc ggttggttgg acaaatgagt ggctaccgcg accatgaatc
    11341 ttgatcacct cttcctcttc ttcgatctcg acacctaatt gttggaaggc atgtaaagta
    11401 ctcagacaat cttctgcgcg caaaaaattt tgtatagtcg tcgttccttc agcaattgct
    11461 ccaaacatga tgctgcgatg ggaaattgat ttatctgctg gtatcctaac agtccctctt
    11521 aatccatgta tttgttgtaa taattgcatt tatccagcct cctatttgat ccagcaatga
    11581 taactggtat tagacataat gattcttttt gcttgtaaca aatcctgttg gtttttaaaa
    11641 ctgatttgca gtattcctaa aatatcttcc ctggtctctt ggatcttcaa attaatcaaa
    11701 gaaactcccg cttttccgat cagtccagtt acttctgcaa taacgccagg ttcatctggc
    11761 acatcgataa ataaatcaaa aaaggcagga atcgcgcctt gcttatggac aggcaactgg
    11821 tctcgacttt cttttgcccg attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg
    11881 atccattctg ctatttgtgt catttcttgt tgccaatctg ccaatcgttc caataatatc
    11941 tgcttattgc tcaacaaaat atctgtccac atgacaggat ctgaggaagc gatcctagtg
    12001 atatctcgaa agcctcctgc tgccagttgt ttggctcgcg gatactcttg attgaattgt
    12061 tctgcctgat tgactaagcc agctgccaga atatgtggaa aatgacttag cattcctgtt
    12121 atacggtcat gttctcttgc tgtaagagtt acatattttg ctttggttcc gcaaaatagt
    12181 tcttgcagtt tttcaacatc ttttttcgtc ttttccaaag gtgtaaagat gtaataggca
    12241 ttttcaaata gtttagcatc tgctgcctgt atacctgatt tatgagaacc agccattgga
    12301 tgtccaccga caaaccgaag atttttctgc tcagcggatg tcacgacttg ctgcttcgta
    12361 ctgctgacat ccgtaatgat gacgtgatca gacaagttga ttctttcaag gacatcaaga
    12421 taagctaagg ttgtacggac gggaacggct aataagataa cgtctgcttg ttctgcacct
    12481 gtttcaaaat cacttgcaac gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt
    12541 tttgactgac tatcccaacc aactactatc agttctggat tggtctgacg aatacaaagt
    12601 gccatagaag caccaatcag tccaagcccc acgataaaaa catgttgtgc catcttattt
    12661 gccccctttt tagtaatgtt tcaagtagtc acggtactcc ttcactgcag ctttcaactc
    12721 aacaagcgaa tcacttggga attttgacaa tagctctgtc gctaccacag tggctacaac
    12781 attttccgca acgacgctag ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc
    12841 tttgtaaggt tcttttgtat cgatatcaac actttgaagt ggcttgtaca atgtaggaat
    12901 gggtttcatg accccgcgaa tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc
    12961 gcctagatta ttggtacgtc ggaaatagcc tctttcttct tcccatagga tctcatccat
    13021 cacttctgag cctggtaatg aaccagaagt gaatccgacc ccaaattcta cgcctttaaa
    13081 ggcattgata ctaaccacag cttgtgcgat tttggcatct agcttcgtgt cccattgtac
    13141 gtagcttcct aatccagctg gaacgcctcc aacgagcact tctacaattc ctccgatcgt
    13201 atctccattt ttctttgtct gatcgatcaa atcttttatt ctttgctcaa ctgctggatc
    13261 aacaactcgt acttctgacg cttcagaaat ttctttgatt tcttgaatcg agatatttcc
    13321 aggtatttct gcttggattc caccaagaca aatcacgtgt cctgctacga tgatgtcaag
    13381 ttcttccaga attttcttgg caattgcgcc gattgctaca cgcatggtcg tctctcttgc
    13441 agaggaccgt tcaagcacat tccgaagatc tcgatgatgg tatttcatac cgcctaccaa
    13501 atccgcgtgt cctggacgcg gacgtgcaac tcgccgcagt gattggtctt tttcaggcac
    13561 ttctgctaca gacatgacag ttgtccaatt tttccaatct ttattttcta ccactaaagt
    13621 gacaggtgaa ccaattgttt tgccgtgacg gatgccagac gtgatccgaa cacgatcttt
    13681 ttcgatcttc atccttcctc ctctgccgta accagcttgt ctgcgagcga gttcgtgatt
    13741 gatttcttct tctgacagag gtaatcctgc tggtagtcct tcaataatgg ctgttaattc
    13801 tggtccatgg gattctcccg cagtcatata acgcatctta cagtcctcct aagtaatctt
    13861 tgaatgtctc tgtgggaaca ggaacgattt ttgcttgccc aatcttttct agcaagatga
    13921 tattcaattg gcttcctctt gctttcttat catgggttat cgcgtggaat aattcatctg
    13981 gatgacttag atcgtacgta atcggcagat ggaatttttg aagtatcctt tctaaagcag
    14041 ctgtcgatcc agttggagac aagccgattt tttctgcatg agaagtgatg gctaccattc
    14101 caatagctac tccttctcca tgactgacta ccccatatcc tgctgtattt tcaattgcat
    14161 ggccaattgt atgaccaaaa tttaaaataa gtcgatttcc ttggtcaaag acatcttctt
    14221 ccactacttt tcgtttgatt tccaacgtag cagtaatgat tggaacagca tgttccagaa
    14281 gatctttttc gtctttcagc tcgttcagct gtttccaaag cgttgcatct gctatagctg
    14341 ctgatttgat gatttcagcg attccttctc gtaaacgacg taccggcaaa gtttttagaa
    14401 cctcaggatc aatcaataca ccagctggct ggctaaatgt tccgacaagg tttttcgctt
    14461 tgtttgtatt gactgctgtc ttcccgccaa tactagaatc tacttgtgca agtagcgtag
    14521 tcggaatttg aacgaaatgg atcccacgca tgaacgtcga ggccacgaat gctgctagat
    14581 cacccaccat tcctccacca agtgcaagta tcccatcgct gcgggtaaaa tcattatctg
    14641 ctaaaaaatc gtataactca gctgcttgtt ctaaagattt actggtctca cccgccggta
    14701 cgattgaaag gactggttca aaattagctt gtctcaattg gaacatgatt ttttctgcat
    14761 atagtggagc aacgtttgta tcagtgatca cagctacttt ttgaggtctc caaatcgttt
    14821 gaagccatga tccagcctgt tctagtgcgc ctttttcaat atgaatatcg tatttttggt
    14881 tatcaacatg tacagtaagc attcgtttca cttctttctt ttaaaaagct ggaattaatc
    14941 gagtgtctga atcttttcca ttgcttcttt catgatcgca acttctttca tcatgcgtga
    15001 gtaagtcttt tcattcaacg attggggacc gtctgaccaa gcatttgcag gatcaggatg
    15061 gatctctacg atcatgccat ctgctccgct tgccacaccc gcacgagcca ttggtggtac
    15121 aagttcccaa attcctgtac catgacttgg atcaacaatc acagggaaat gagttaattt
    15181 tttcatcaat gggacagcac ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg
    15241 aatcccacgt tcgataaaaa tgacaggaga tttgtcttcg actgcaatgt attctgcagc
    15301 attaagccat tcattgatcg taccggaaat tccacgtttc aatccaacag gtttacctgt
    15361 tttaccgaca gcagatagca gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat
    15421 atcgctgtac tcagccacca tatcgatatg cccttcatcc atcacttcag taatcacttt
    15481 cattccaaat tcatcagccg cttgacggat gtatttcaat ccttcttctt ctaaaccttg
    15541 gaaagcatat ggagaagtac gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc
    15601 ggctttggcc attcgcgcac attctctgat ttggtctaat ccttcgatag aacaaggacc
    15661 agccatcgtc acgaaactgc cgtcaccaat ttttactccg tctacatcca cgactgtatt
    15721 gccaggatgg aattctcgag aagtcaattt gtatgtgttt aaaatacgca cagcattttc
    15781 tactccttca tagcgtgtaa atgctccttc ttgtaaactt cgcgtatcac cttttagtcc
    15841 aatcactact cgttctgttc cttgatcgat tgagactttg aatccttctt tttcgatatg
    15901 atcgacgact ttttttattt gttcttccgt tgcttctttt ttcataatta ctatcattgt
    15961 ttttcccgct ttcttttgtt tacttatcta tcttggctga ttttctctac tttttcaagg
    16021 tggttttaca ttaattccta ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc
    16081 tttcctactt atgacccaga taatcaacaa aaaaagatgt ccctgtagga aattgcctct
    16141 acagtgaaca tcttcaacga gccttaaaaa gcgctgcaaa aatataaaat agccactata
    16201 gatgaatttc tctacgtggc tgtaagtcgt gtttaaaaat ctttagccat catagataca
    16261 aacctggttt gtttgtaccc acgaagtcat gaatacaaac ctatctaaaa tagctaaagt
    16321 aatacgtatt cagttttgta agttttctag atttcataaa aaatcgctcc aaactgatgt
    16381 aagaattgta cctagtatag acataaaaaa atcgactgtc aatcattttt atgaaacttt
    16441 ttttaatttt ctgatagtta actttgtttt tcagaaaaat aacaattggt tttattgaag
    16501 aaaatagtca tgaacaaaag tatctgagaa cttttttact ctcttgttca tgacatatag
    16561 aataaagcag ctttgtcttg taaactccgt ttttcaaata ataatctaaa gattgctact
    16621 gaataataaa gccgatttca tggtcctctt cgctgctgat tcggtaaggt gactcaacat
    16681 ccgctcccca gctattgata cccccgactc ctcttacttt tgcaaaaatc gtaagaaccg
    16741 ttcgtctttc tggcggtagt tcttctatat gtgttgcctg ttccagttct tctgctttga
    16801 atggtaaagc ggaaaaagca aatggcgtat cctgctgttc aaatcgcaac gaaaaatctt
    16861 cttgatcttt atccgtatta tccaacgttt gatttcttgt tagttctacc catttcgttt
    16921 ccatatgcat gccgcattct tggggaacaa ggtatgggga aacggggagt cctgccactt
    16981 tataaatacc ttcgacacca ccagccatcc gatccggata tgtttcccct gataggcctt
    17041 catactcaaa aaattttgct ttcgttggca taatgaacct taaacccaat gcgggtaatt
    17101 ccggtaatcc tttttgtcca taataatgca tattgacaag cattttaccc tctccagcga
    17161 tttggtagga aattgtcact tgggtttttg gtactgtgtt gatttccagt gtaaaaatga
    17221 tttcaaccgt ttcagcgtat tcattacttg aatattgatt atttactgga gcaatcggca
    17281 aatcgatctt tttattgtcc accagtactt ctatttcttt gacttttgga tacaatccgg
    17341 ctcctaacca cataccggat ttaaatccaa atccgctacc tctgtcatta tcggtcgttg
    17401 ctcgccaaaa aactggaagt gtttcccggt atagccattc ttttccgttt ttattgagcg
    17461 attccagtcc gcctcttgta taagaaaaaa tataatgcgt attccccgat ttgacaccta
    17521 atgtacagtc gccgaagacg attgcaactt tttccgtatc catatgactt ttatttactg
    17581 tatttttcat aataatatct tacctcctgc atggcgggtt tttcttgtcc atctgcaaaa
    17641 acgatcccat tccctgagaa ttcatagtca cttggtttat cgtcaaagtc accgccatag
    17701 cgcatcactt ctctgcctgt gattggatca gtcacttgga tcgcctgatc tttaaaatcc
    17761 catatatagc ctccttggta catttcataa cgatcaagca acgtcatgta agaatccatt
    17821 ccacctaacg aattccccat gtcatgcata tattcacata agatgaacgg tttttccgct
    17881 ttattttcaa ggtagtcaat gatctcttct ggtggcgcat acatgcagct ttttacatct
    17941 gaaatcgaat cttcgaatgc tggatttgga aaattccctt cataatggac aagtcgatcg
    18001 gggtctactt ttttgaagta atcgttcatt tgtcgaatgt tttcttccgc ataagattca
    18061 tttccaagag accaaaaaag aatacttgga tggtttttta atgtttcgaa attcgttttt
    18121 gctcgatcaa gaacagccgc ctgccactga ggtactgcac ctggtacgtt gtaagaaggc
    18181 tcaaatgcgc ccattttttg ccatgagcca tgggattcga ggttcgtttc cgccatcaca
    18241 tggattcctg cttggtcgca taaataatac caagtgatct ggttggggta atgagatgtc
    18301 cgtacgccgt tgatctttgc attttttatg atagccaaat ctttttccat atcttctata
    18361 gtgatggcgc gtcctttttt cggatgccac tcatgtcgat ttaccccgtt gataatcatc
    18421 cgttttccat tgaagtagat gattttatct ttgatttcga ttttacgaaa accgaaggaa
    18481 tagtcaatat actccgttac tcttttttct tcgtctatcg tctccaattc aagcttatat
    18541 aaattgggct gaaaatgccc ccacagtgcc acattttcaa atccatcgat gcgaatcgtc
    18601 atttgctctt ttaacggata ttgctgttga tagatttcct tcccatcagg atctagtatc
    18661 gctacattca gctgactgtt tttggctact tgctcaaaag acaaatctac ttcaagtttg
    18721 ccattttgac atttgtcatc taagcgaggt ttgatccaaa gatcttctaa atggttttgt
    18781 cctcgagcaa ctaagaagac atctctgaag atcccaaaaa atcggaaaaa gtcctgatct
    18841 tccaagaatg cagctgtact tcttttgtaa acctccacag ctagaatatt ttcttcttcc
    18901 aaataaggtg ttaaatcaaa ttcagacgga gtgaacgaat cttcggcata cccgataaaa
    18961 tgaccattca accaaataaa cattgcttgt tctacaccag caaaatgaat cgagatttgt
    19021 tttcccttta gaccttcttc taatcgaaaa gtcgtcaaat acgaaccaac cggattgtct
    19081 ttagcagctg aaaagatccc ttccgcctct ttctcagggc ttgtcgtgaa agggggacgg
    19141 cgaaatacat gtccctccca cggatacatc gtattgatgt aatggatctt gtcataattt
    19201 gctaattcaa tatgactagg aacagtgatt tcatcaaaag aacaggcatc aaaatctttt
    19261 ttataaaatg tttttggtcg taccgaagga tttggcgaat aacaaaaacg ccatgatcca
    19321 tttaaagact gtttaaggga ggatgtggtt tctacttctt cccttgttgc ataacaaaca
    19381 tgatcgctat gtgcatttga ttggttcact ctaaaaactt gtggatcatc taaccatttt
    19441 atatttgctt tcatactttt ttccttctct ctcataactt tttgaaatga agcagttctg
    19501 tttggaaatc accttgaaaa tcggggaagt aaagtccaac tgtcatcaat tcggcccccg
    19561 aaaatatccg cttcgaggtc tgttcctgat aaagtgcctc atcatctaac cccatcagat
    19621 atacttcatg gaacgctggt tgagctgaag ctagtatcct ccctaaaaac acaatagctt
    19681 catcttgttt aggagacaca aacatccatg cggcaatatt ttcttcaaaa ggactcgcca
    19741 aacgataata atggccatac tggatcaccg gacgaatcgt ttgataggat ataatttggt
    19801 cttttacttg ctttttttct tcaggagaca gctttgtcaa atccagttca taaccaaaaa
    19861 cagcgctcat agctgtttct cctcttgttt tcaacgaagt agatctgtcg gtctgatgat
    19921 ttggtacagc tgatacatga gctgtgatgg acgaaatcgg ataagcaagg cttgttccat
    19981 attggatttt catacgctcc actgcatccg tgttatcact tgtccatgac tgcggcatat
    20041 aataaataaa cccggcatca aaccgtccac ctccaccaga acagccttcc cataagattt
    20101 ccggataatc agttgtcaaa tgttcaagta gttcataaag cccgagcata taacgatgcg
    20161 ctacttctcc ttgtctctgt gggtcaaggg taatcgaata gacatctgaa agcgaacggt
    20221 tcatatccca tttgatgtag tcaagaggaa tcgtatctaa aatctttctc atttgtttct
    20281 caatcgtctg ccgaacatct aaccttgtaa aatccagtac atgttgtgat ctggaagcag
    20341 aaggcattct acctggctcc tgcataagaa aatcaggatg tgttcgatac agttcagaat
    20401 caaccgagat catttcaggt tcaaaccata aaccaaattt caatcctttg ctatgagcat
    20461 aatctgctat ttcacgtaat ccatttgtca acttgccttc atactcgaac cagtctccta
    20521 atgaagaatt gtccgcatct ctttttccaa accagccatc atctaatacg aacatttcga
    20581 tgcctagctc ctttgcttca tcaatgatcg ccttgatttt ttcactattg aagtcaaagt
    20641 aagtggcttc ccagttattg accagaatcg gccgttcagc aaattgatga cgacctcttg
    20701 cgattctttc acgtagcaac tcatggtgga tctgactcat tttgttcaat ccctgacttg
    20761 agtaagaaag gatcatttca ggtgtttgaa aagactggcc tgcattcaat tcccacgaaa
    20821 aacgatggct gttgatccca ccgacgactc tcaattgatt gatctgatct ttttcaagtt
    20881 cgaaagaatg gttgccggaa taaacaaatt gaagtccgat cgcttctcca gtattttctg
    20941 tcgtatgatg atgaactaaa gcaataaagt tattcatatg gtgactgctc gttcctcgac
    21001 ggctttcaaa tacttttgtt ccagataaaa cagattgccg tgaaatctgt ctttcattga
    21061 catgtgctcc tggcaaagca atgacttcat caaatcgcct agtatgggca aagtctagct
    21121 ggaaactagc cgctttttct agtttgattg tttctccggt ttcatttctt atctttacag
    21181 atcgtgtgat cactgctcta tcagtaaata tggtatagaa caaatcgaaa taaatcgctg
    21241 tttctctatc cttaagtgaa atcaccaacg tttgtgcctc tgacttgtcc atcacataag
    21301 tttgaggcaa gccttcgatc tctggcttcc catctgtaat ataatggctg tcatatctca
    21361 gatccagtaa gtctgatcca ttcttgcgct gaatgatcga tgcgggcgtt cgaaagtcca
    21421 tgctgccatg agaagagtat tcttgcggta gtgtatcttt cgacagagac cggtcaggat
    21481 ttaagggaac atttcctgaa aatccacgat ctcttcgggg ataattttta ttgtctttgt
    21541 actgcttgac tggtttgcca aaataaatat gcgccaatac attgctttct tctatcttta
    21601 tcagataaga aagaaatgta tttcgtaaat ggaagactcg gctcttttca tcaaataaga
    21661 tgaaattttt atctgttttt ctatgcgtaa tatccatcat ttcactccca acaaaacgga
    21721 aaccgtttac ttttttttat aattcctgta tactgtttac taaacaattt tgtcaataaa
    21781 aattttttat ttttttgagg agtgtgtaaa aatggttgga attagagatg tcgcacgaaa
    21841 ggcaaatgtc tctcctgcta cagtttcccg tgtgttgaat aacgatcaga cgatccatgt
    21901 ggctcagact actcggcaac ggatagtaga ggcagctgct gcgttggatt atgacatcag
    21961 tactcgtaaa tatatcaaaa agagaatgcc ttcgattggt gtgatctcta cgatcggcaa
    22021 agcgagcgaa gaacaggata tctattacaa acatttgcga gtagggatgg aagaagaagc
    22081 tcgacgtctg catttaggga tgaatcgaat ctataatcta tctgataatc ctaaacaatg
    22141 ggaagatcta gatcagttag gtgcggtgat cgttgttggg actgttacga aagaatcgat
    22201 taccgccttg ttaaaacaaa acatgcaaat agtcgtcgtg gataaccctg acatccagca
    22261 agaggtcgat atggtgtatg cagatcttga acggatgact aaaaccgtac tggaattgtt
    22321 cttagaaaat ggtcataaaa gaatagccta tatcggggga tataaagtcg atatggatga
    22381 attcggtggg aagcagattt ctataaatga aaagaggatg cgtgcctatc agcattttat
    22441 gatcgatcat cacttagaaa aatatatgtc ttatcatcta ggtggctgga cagaaaaaca
    22501 aggtgaggta cttgcgacta atctattaga aaatgaatcc cctcttccta ctgccattct
    22561 tgtcgggagc gatccaatgg caattggtgt ctatcgggca ttgaaacaaa aaaatatcca
    22621 aattggagaa gagattgttg tagcaagctt tgataatatc gagacaatcg caaacctctc
    22681 ccctggcttg accagtgttc aaatcaatgc gaaatcaatt gggcaagcag ctgttcgaat
    22741 ggcattggag cgaattgacg gtatacggga cgaaccaatc attttgactt atcctgcacg
    22801 attgattgtt agggaaagtt ttatcccaag aacaaaaagg tattgaaaga gaggttgtga
    22861 aaagccgctt cgtttcttca tactcaaaac gaaacggctt tttcacaacc tcttacttat
    22921 acttattcac aaaatccaat gtctgctggt aacaattttg cgattctttt agcgtgtaat
    22981 caaattggta ttcatgggta atctcttttt ttgtatctgt ataaaacaag gattgcacgg
    23041 gtacctttaa ttctgtcaac cgattttcta atgcttgacc ttgctcctgg aatgagtatg
    23101 catttccgtc agttatatac gttggcggaa attccttaga aacatggtca gccacactag
    23161 cttctttcaa ttgctcactc gttttccaat cttttgttcc aatcaacgac cacgcaacgg
    23221 ttttgacgaa aaacttcata aatcgatcat ccgaatgctg ctgtgctgtt agcttcaaat
    23281 caacaggtcc ccaataagaa atggttcctt tcagactatc ttttggaagt agctgtttga
    23341 ttttcatttg tttagcatac gcatcattcg tttgtatagc tgcatattgc aatgcgattt
    23401 gtgaacctgc actatctcca ccaaaaaata attgttccat gtctagcaat ttgtcgtctt
    23461 tttcttcagc taataaatct ttgatcagtt cattcacttg aagaacttga ttagggtatt
    23521 ctgaatcagg cgctaattca taattcatcg caaccactgc gatgttggcg tctgaaacta
    23581 attttgtcgc aaattctttg acacccgatt tatctccgcc gacgaatcct ccaccatgta
    23641 cccaaaatag aacaggaaca ggccccttgc tgttttttgg atagtagatg tcataagtat
    23701 tctctttata cttggactca tacgtcaaat ctgttttttt cgccacgttt ttttctgctt
    23761 gatcgaacgt ttttttatca gtgatttgaa cagtatttga aaacatatgg ttgatcaaga
    23821 atgctccagg tctgggggaa agctggaagg caagaaagat tgcaaacgcg atcccaagaa
    23881 tgattccgaa gaaccatccg ataaatttaa gaattttctt tttcataagg ctccctcagt
    23941 agttagtatt aagcattctg attataccat agtgttattt cctatcaagc tctcctagat
    24001 gatttaatga ttttccccaa acttcttctg caatgctttt gacaaaagaa attttttcca
    24061 ttgttcttca aacaaaacat tttcttcttc tgttgaagca aatccacatt gaggacttaa
    24121 gcacaaacga tccaaaggca gatatcgtgt cgcttcgtag atccggtcaa tcacttgctg
    24181 gtgctcttcc atttcgccat ttttcgttac agtcaaaatc taagacaact aatttatctt
    24241 cagagacttt ctctaaaaga acaaaactcc cagcacgttc actatcgtat tctaaataat
    24301 aggcacggac attttcttga gtaaaataat gggtctgcta cgtcgtcatc ccccctgatg
    24361 ccgccaaaat cgaataataa tttccgcgtc aaacatgtat atttagtgtt gaatcatttg
    24421 agaatatgaa aataaaaatc tttttgatca atcagttaat caaattgaaa taacgaaaaa
    24481 tctgagacag aatgacgttc attctgaccc agatttgatt ttgcacaact caacagtcct
    24541 tattggcaaa tcgccaaaat aatgtagact aacta
//
LOCUS       DARBDV010000037        23831 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000037 DARBDV010000000
VERSION     DARBDV010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.037"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(55..789)
                     /locus_tag="SCM56_001401"
     CDS             complement(55..789)
                     /locus_tag="SCM56_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEG1110187.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1116..1526
                     /locus_tag="SCM56_001402"
     CDS             1116..1526
                     /locus_tag="SCM56_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEG1110188.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1519..2220
                     /locus_tag="SCM56_001403"
     CDS             1519..2220
                     /locus_tag="SCM56_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEG1110189.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="SCM56_001404"
     CDS             2220..3806
                     /locus_tag="SCM56_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1110190.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="SCM56_001405"
     CDS             3825..4046
                     /locus_tag="SCM56_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEG1110191.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="SCM56_001406"
     CDS             4043..4804
                     /locus_tag="SCM56_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEG1110192.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="SCM56_001407"
     CDS             5184..5693
                     /locus_tag="SCM56_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEG1110193.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="SCM56_001408"
     CDS             complement(5768..6307)
                     /locus_tag="SCM56_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEG1110194.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="SCM56_001409"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="SCM56_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEG1110195.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7320..8171
                     /locus_tag="SCM56_001410"
     CDS             7320..8171
                     /locus_tag="SCM56_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEG1110196.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8199..8834
                     /locus_tag="SCM56_001411"
     CDS             8199..8834
                     /locus_tag="SCM56_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEG1110197.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="SCM56_001412"
     CDS             8854..9627
                     /locus_tag="SCM56_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG1110198.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="SCM56_001413"
     CDS             10035..10832
                     /locus_tag="SCM56_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEG1110199.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="SCM56_001414"
     CDS             10810..11223
                     /locus_tag="SCM56_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEG1110200.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="SCM56_001415"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="SCM56_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEG1110201.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="SCM56_001416"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="SCM56_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEG1110202.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /gene="maf"
                     /locus_tag="SCM56_001417"
     CDS             16000..16560
                     /gene="maf"
                     /locus_tag="SCM56_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEG1110203.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="SCM56_001418"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="SCM56_001418"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEG1110204.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="SCM56_001419"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="SCM56_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEG1110205.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="SCM56_001420"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="SCM56_001420"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEG1110206.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="SCM56_001421"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="SCM56_001421"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEG1110207.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="SCM56_001422"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="SCM56_001422"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEG1110208.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="SCM56_001423"
     CDS             21907..22485
                     /locus_tag="SCM56_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1110209.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="SCM56_001424"
     CDS             22537..23019
                     /locus_tag="SCM56_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEG1110210.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="SCM56_001425"
     CDS             23136..23510
                     /locus_tag="SCM56_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110211.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7783 a         4285 c         5114 g         6649 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca
     1141 ttattatttt cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc
     1201 ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc
     1261 ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc
     1321 ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac
     1381 tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag
     1441 gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcacccgg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatagaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaat aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcatagca tgacagcatt
    23641 cggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cattagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagttt t
//
LOCUS       DARBDV010000038        23488 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000038 DARBDV010000000
VERSION     DARBDV010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.038"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..310
                     /locus_tag="SCM56_001426"
     CDS             <1..310
                     /locus_tag="SCM56_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1110212.1"
                     /translation="QIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNL
                     IEGFNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF
                     "
     gene            783..1337
                     /locus_tag="SCM56_001427"
     CDS             783..1337
                     /locus_tag="SCM56_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1110213.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(1510..1983)
                     /locus_tag="SCM56_001428"
     CDS             complement(1510..1983)
                     /locus_tag="SCM56_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1110214.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1995..2531
                     /locus_tag="SCM56_001429"
     CDS             1995..2531
                     /locus_tag="SCM56_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG1110215.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            2571..2942
                     /locus_tag="SCM56_001430"
     CDS             2571..2942
                     /locus_tag="SCM56_001430"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG1110216.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            3226..4497
                     /locus_tag="SCM56_001431"
     CDS             3226..4497
                     /locus_tag="SCM56_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEG1110217.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            4742..5767
                     /locus_tag="SCM56_001432"
     CDS             4742..5767
                     /locus_tag="SCM56_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1110218.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            6061..7170
                     /locus_tag="SCM56_001433"
     CDS             6061..7170
                     /locus_tag="SCM56_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEG1110219.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            7253..8149
                     /locus_tag="SCM56_001434"
                     /pseudo
     CDS             7253..8149
                     /locus_tag="SCM56_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            8165..8989
                     /locus_tag="SCM56_001435"
     CDS             8165..8989
                     /locus_tag="SCM56_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1110220.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            9021..10331
                     /locus_tag="SCM56_001436"
     CDS             9021..10331
                     /locus_tag="SCM56_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HEG1110221.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            10479..11738
                     /locus_tag="SCM56_001437"
     CDS             10479..11738
                     /locus_tag="SCM56_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEG1110222.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11748..12620
                     /locus_tag="SCM56_001438"
     CDS             11748..12620
                     /locus_tag="SCM56_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1110223.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            12632..13462
                     /locus_tag="SCM56_001439"
     CDS             12632..13462
                     /locus_tag="SCM56_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1110224.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            13502..15685
                     /locus_tag="SCM56_001440"
     CDS             13502..15685
                     /locus_tag="SCM56_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEG1110225.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15746..16966
                     /locus_tag="SCM56_001441"
     CDS             15746..16966
                     /locus_tag="SCM56_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HEG1110226.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16963..18423
                     /gene="gtfA"
                     /locus_tag="SCM56_001442"
     CDS             16963..18423
                     /gene="gtfA"
                     /locus_tag="SCM56_001442"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEG1110227.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            18500..19606
                     /locus_tag="SCM56_001443"
                     /pseudo
     CDS             18500..19606
                     /locus_tag="SCM56_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19928..20137
                     /locus_tag="SCM56_001444"
     CDS             19928..20137
                     /locus_tag="SCM56_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEG1110228.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            20130..20279
                     /locus_tag="SCM56_001445"
     CDS             20130..20279
                     /locus_tag="SCM56_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HEG1110229.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            20433..21098
                     /locus_tag="SCM56_001446"
     CDS             20433..21098
                     /locus_tag="SCM56_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1110230.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(21382..22467)
                     /gene="xerS"
                     /locus_tag="SCM56_001447"
     CDS             complement(21382..22467)
                     /gene="xerS"
                     /locus_tag="SCM56_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEG1110231.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            22801..23034
                     /locus_tag="SCM56_001448"
     CDS             22801..23034
                     /locus_tag="SCM56_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HEG1110232.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            23031..23438
                     /locus_tag="SCM56_001449"
     CDS             23031..23438
                     /locus_tag="SCM56_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEG1110233.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
BASE COUNT         8050 a         3510 c         4471 g         7457 t
ORIGIN      
        1 tcaaataagg tttatgatag ataaatggaa aaagcagtat ccacgagtgg ttaaattgct
       61 catgaatcct gctatattaa ccttttataa tttccctccg tcaatcagaa gaactattta
      121 ttcaactaat ttgattgaag gttttaacaa acaattaaag aaatatacca aaagaaaaga
      181 gcaatttcct aatgaagaat ctctggagag atttctagta tctcaattca acaactataa
      241 tcaaaagttt ctatgccgag ttcataaagg ttttaaagaa atacatgata cattagaatc
      301 aatgttttaa cttgatcaac agaatgggct tttccattta cacaaaatta ttgacgctcc
      361 cgacaaaatt atgatcttgc gtaaagttat aaaatattaa taaaaatatt ttattaatga
      421 aaagcttggt agcacggtta ttcatatgct aatttatttc actttatagt aaggggagtt
      481 cttagtatat tgttcaaact taaatttcat ataatttaat tatgtaaatt agctattcta
      541 agaacttcta cggataactg cccgtctaaa atttaaataa gagcttagaa tcttcatata
      601 agtaaggtta atttttttgt acaaaaaagt cggaatattc tgagttactg aaatgatata
      661 aaatcataaa ctaaatccct tgattttatt gactttcaaa agtttatata tataagttaa
      721 tgattatggt aaaatatata cataatcatt aactcagaat atttttaaga agggaaacaa
      781 tagtgagcta taatgtacaa ccgttaagga caaatgaaga gatacaagat tttcagtttt
      841 ggctaagaag aacaagtaat ccggaaagag atagtttttt atttttgttt ggaatcaata
      901 atgggttacg tatgtcagat attataggat taaaagttgg agatatacga ggcaaaagta
      961 agcctattat tgttgaacat aaaacaggta agcgaaaacc gatttttata gataatttga
     1021 gagaagaaat tttattatat actgaaggta aagaagaaaa tgattggtta tttccgagtc
     1081 ggcaacaagg tcgacatata acaagggatc gtgtctatca gatatatgca gatatagcag
     1141 aaaaattagg acgagatgat ataggaacac atacattaag aaaaactttt gggtatcact
     1201 actacaagaa aactagagac attgctacct taatgtttat ttttaatcat agctcgcaag
     1261 caattacaaa aaggtacatt ggtataactg aagatgaaat aggagcatca cttcgtggat
     1321 ttaaactagg tgtataattt aaaaattttt ataaacagta aagacaaaga ttaaaagcta
     1381 tttttatcta gaacgatttt atttcataaa gttcgcggaa taaaagctgg ggttactggt
     1441 acttggtata ggttcgtgtt atataaacta agtaattaaa taacattttt aggttggaaa
     1501 aggatgaaat taaaaaaaga gaatcaatac ggtctcaaaa acgcaaacgc tgcggaataa
     1561 gaaaaatact gataactcaa cgtttagcag actatctaat agggatagtc tgcttttttc
     1621 gtttttgacc atacttttga ccttctgcaa acttccttgc ttttcctctg tagaactcat
     1681 gaaatctgca aaacgaattg ccgtttcctc tgtctgcttt ttcgcgatat gggtgtatat
     1741 atcatccata gtggttttaa tatctgtatg tccaagtcga acatacactt ctttgacaga
     1801 cgcaccagat tcaaagagca gactacaatg ggtatgacga aaaccatgaa gtgtgatatg
     1861 ttttaagtta ttctctttaa tgatgatttt caaattgtga tttacatagt ctaaataaag
     1921 gggcttattt tcttctgatg aaaacaaaag ttgttcacca tcttttatag gtttgattct
     1981 catttttaga tattatgccc gcacgtattc atcacatttc tattgtgaat cgctttattc
     2041 gaccgacatt tgacttttat cataatatac tcggtttaaa attactaatg aaaacaatta
     2101 atcaagacga tcataccatg tatcacctgt ttttttcaga taatcaccac cgtgtgggaa
     2161 ctgaactgac tttttttgaa gtacaagaag ggaacaacca gttttttgga accaatacga
     2221 ttgaacgaac gatactaaaa gttccttccg aagcatcatt gcatttttgg gaaatatatt
     2281 ttgaaacaca aggcgtttgc cattatggaa tcgaatcatt ttctggacga cctatcttac
     2341 gttttgaagg gccagatgca acacgattag ctttagttcc cttacgcgaa tttgaagata
     2401 ttgatgatta tttcccgtat gtcacagacc agatccctac agagcatgcc attttaggaa
     2461 ttgatgccat tcaactacgc gtacaatatt ccgatgcgac tgaacgagag ttattgtcgg
     2521 taggctggta gcacaaagaa tacgtcagtt tcttttcggg tcatcaacga gtgacaatct
     2581 tagaaaatca agacactcag ttctatcaag aagttcagat tattcaagat cgtgagaatc
     2641 cagttgccat tgaagggatt ggtagcgtcc atcatgttgc gtttggcgta gaaaataaat
     2701 ctgatttaca acgcatcgac aaacaattac aagagcgcaa ttttataaat tcaggaataa
     2761 aagatcgtga attttttgta tcactttatt atcgtgaccc aaatcaatta ttgattgaaa
     2821 ttgctacagg ggaaggaaac ctagatgcaa aagcgtatga aaatcaaagt ccacgctttg
     2881 aagaaattcc tttattttta ccgcagtact taaatttcat tcgtgaacaa gtggagcaat
     2941 aattagctaa tcaaatccat gacgaagagt aagaggttga ctcataagtc aaaaaataaa
     3001 gaaaaacgca tcaaatccat tctaaagaat gttgatttga tgcggttcct atttttataa
     3061 actatcattt tcgactaata aaactactta tgagtcagcc tctctttttt tcttgatttt
     3121 tatacctctt tctaggtaaa ctaaacatct gccaaaaata ccgtgtctca ttgaaacatt
     3181 ctcaactatt tcaaaataaa ggggagtacc aaatgaacga gtatattgaa attatccacg
     3241 caagagaaac aacttaaaaa tgtcagtctc aaaattccca aaaataaaat tagtattttt
     3301 accagcgtat ctggttcagg aaaatcatcg attgtttttg atactattac ccaagaagct
     3361 ggtcgtcaac tgaatgacac ctatgatagt tttacccgtt tatttttacc taaatacagt
     3421 cgtccagagg ttgatgagat tgataatctt tctacggcga ttgtggtcaa tcaaaagaag
     3481 attggtggca gtgcgcgctc aacgttaggg acgattacta acatcaatgc gttatttcgt
     3541 gtgttgtttt catgttttgc aacacctagt ttaggatatg caaatgctta ttcatttaat
     3601 gatcctagtg gaatgtgttc tgtctgtcag ggaatcggta agacgattgc atttttaata
     3661 ggattgcttt atttttcggc tattgataat gctgattttg tattttctga tccatttacc
     3721 gcagaagttt tgtctggaat tgaggaacag gcaaagaatc atggttattt tgtattggtg
     3781 cataatgtta attcagcaga agatgttaaa actattcaga gaaattggcg ttttgagggg
     3841 ttcattgttg ttggtgtacg tgtaaaagaa tttttggaat taaataacgt ttttgatggt
     3901 cctgttgttt atattgatac gcatctttca aaagagacat taagagaact tgaacaacaa
     3961 atgaatcgtt tatttgtaaa tacaaatgat taccaagcta gtacattggc agtggaacat
     4021 ttgattcatt atggtcatga gaaaatagcg tttctttctt ttgactacat tgtaggtgaa
     4081 ccaagtgtta ttcaagaacg acatcaagct tatatcgata cattaaagaa atatcattta
     4141 acctttaaat cgcattggat atataacaac tctgaatttg agcgaattta tcaagaatta
     4201 gctgaattta ctgcaattgt tgttactgga gattatttag cggctaaatt agtaaaattt
     4261 ttaaaaaata agaataaacg agtgatggaa caattatcga ttattggatt tgatgatatt
     4321 acttttgcag aattaatgga tcctgcgttg acaacaatac gactaaatcc aggtaacaag
     4381 ggggcgattg cttttcacca actgattgga ctggccaaac aagaagaaat aaaaaatcaa
     4441 gtactactat tagagggaga attgatttct cgagactctg ttcacaaaat caaataaaaa
     4501 agctctgtga aataagtata gttaacaata aaatatccga acgatagaga gaatgaactg
     4561 tgatctagca gttcctcgtc gcaaattcac tagaattagc attatttact ctaaattgtt
     4621 cggatatttt ttcttgtttt taattatgat tacagtgcct atttttaacg aaatttgctt
     4681 attttcaaaa tgtatgatta tatgttagta aacactttca aatatgaaaa gaggagctat
     4741 tatggtagga attagggatg ttgcaaaacg ggcgggtgtg tccattgcaa cagtatcgcg
     4801 cgtgctaaat gaggatccga cactttcagt aaccgatgaa accaaacgaa agattgcaga
     4861 atcggttaat ttttatcatt atgtcaaaaa aagtcccgtt gcaaaaaatt taacaaatat
     4921 tgcattaata acgacagttt ctgaagtaga tgagttggaa gatccctatt ttcgaacgat
     4981 tcgcagaggg attcaagtcg aagcagaaca aagcaaagtc aatttgaaga aaattattcg
     5041 tttgtcagaa acaccgttgg agcaagacga tttgctaaaa tgtcaaggtg tgttgattat
     5101 tgggcaggtg ctatcttctg tgattgaaga agtatcagca attaattcga atgtcgtagt
     5161 gattgatgac cctagtattg aagctgaagt agatgcagtt tatactgatt taacaaaagc
     5221 aacgaatcag catttagatc gattgtataa aaaaggacac cgaaacattg cgtttatcgg
     5281 aggaggacgt gttcgtttag atagccaagg aaataaacac gtgtcagatg atgattatcg
     5341 aaaaatagcc tatgagacgt ggatgaaaca aaaaggatta gaaaaatata gtcaaattta
     5401 tttagaaggc tggaccacac ttgacggaat gcaggcagca gatttacttt tgaaaaaaca
     5461 tgctaaagat aagttgccga ctgctatcat ggtaggaaat gatcctattg ctgtgggggt
     5521 ttatcgttca cttcaaaaaa atggtctaca tattccagaa gatatttcga ttgtgagttt
     5581 tgataatatt gaagttgctg aatttttaac acctgcatta aatacggtga atgtgaatac
     5641 agaagaaatt ggaaaactag cagttcgcat ggttaatgaa cgaataaaag aagttagaaa
     5701 tgtttcgatc aaagtgattg tttctaataa tattattgtt agagaaagtg aaaaaaatat
     5761 cgattaaaaa agtaaacatc caaaaaaggg tgtttacttt tttgtttact aatattttta
     5821 gtaaaaaatc tcgacaaaac attaagtaaa cagttatggt gttgttaaca aaaagaaaac
     5881 gcattcaaaa caggaggaat taagatgaaa catttcaaaa aaatagcttt aacagggata
     5941 gctgttttaa ctgggacatt actcgcagca tgtggtgggg gagatagtaa ttctaaagac
     6001 gggcaagtaa ctttaagcta tgcgagttga gatactgacc aagctccagg actaagaaaa
     6061 atgttagatg aatttgaaaa acaaaatcct gatatcaaag tagaaatgga gacaacgcct
     6121 tgggatcaat attgggttaa attagaagct gcaacaacgg gaggtaatat gccagatgta
     6181 gtaaccatgc actctagcga atcctataaa tatatgtcac agaatgcttt gatgggactt
     6241 gatgatgtta tcaaagaaaa tgatattgac atgggaaact ttactgaagg aattgctgat
     6301 ttctatacgc ttgaagacga actatacgca atccctaaag atgcttctgt agtggggcta
     6361 tggtacaaca aagaattatt tgataatgca ggtgtcgatt atccagatga aacatggaca
     6421 tgggaaacac tcagacaagc tgcaattgat ttaacagatg aatcaaaagg catctatggt
     6481 tttgcatcac atattggaac agagactggc tactggccat ttatttatca aaatggggga
     6541 gaagtatttg ccgatgacaa tacaaaatca ggtttgaata ccccagaagc acaagaagct
     6601 ttagcttttt atcgagatat tgcacttgtt gatcatgctt caccaacaat tacagaatta
     6661 caagatcctg ataagttaag tcgttttcaa tcaggtagat tagcaatgat gattgaagga
     6721 aactggtttg caccgggatt tttgaaaaat gactatctag ttgaaaatgt tgcttctgcc
     6781 gtgttaccta agggaaaagt taatgcaaca attacaaatg gacttggttg ggctgccagt
     6841 gcgagtacaa aatacccaga agaagtgaaa aaactaatgg catttttagc tagtgaggac
     6901 gccaataaaa tccaagcgga aacgggggca tcaatcccag cactaaaagg ctttgaagac
     6961 gagtgggctg cttcttcaga tacaattgat ttgagtgctt ttggaaaaat gttggagtat
     7021 ggagtccctc gtccatacaa taaagatgga ttacgagctg aacaagcaga agttcaaatt
     7081 attaaccgta ttctgtctgg tgaagtaact gttgaagatg gaaccgctca aattgaaaaa
     7141 gaagtcaatg cgatattgag cggaaattaa attagttaat tatacaatat acaggcgaaa
     7201 ggattctatt tcgtctgtat attaatggat gaataccaaa aagggggata ttatgaaaaa
     7261 tcgaattttt ggaaaatatc gtaaagatca agtgatatgg ggctatattt ttatttttcc
     7321 aacggcatta ggattaattt tattaaatat atggccagca attcaaacag caattttaag
     7381 ttttcaaaaa acggtagggt tcggtgatac tgaatgggtc ggattaagaa attaccaaaa
     7441 aatgtttgca gacggtgaag tttttcagtc tttattaaac acattaattt atgcagtggt
     7501 ttcgatacct agtattgttg tattatcctt gttggtggca gttttaatga atagaaaagt
     7561 taaaggattg tcgatttatc ggacaattta ttttctacca gtcgttgctg caccatccgc
     7621 agtcgcaatg atttggcgct ggatgttcaa taatgattat ggactgatta ataacatgtt
     7681 ggctaaaata ggtctcgatg gaccagcatg gttaacggat ccatcgattg cgatttattc
     7741 tattatcatt gtaggagttt ggagtgctat tggttataac atggtgttat tactagccgg
     7801 cttataagaa attcctaaag attattatga agcagcggaa attgatggtg ctagtccaat
     7861 ccagcagttt ttcaacatta cgttaccatt agttacacca acattatttt ttgtagttgt
     7921 aacaacagtt atcaatgctt tccaagtatt tgatgttatt tttatgatga ttactcctaa
     7981 tagtaccgcc atgtttaaat cacaatcatt agcctattta ttctataaac attcatttat
     8041 tttaaatgac aagggatatg gttcggcaat cgttatgttc cttttagtga ttatattaat
     8101 tattactgca attcaaatga agttgcagaa aaagtgggta aactattaag gaggctatca
     8161 aattatgaaa aataaatttt tttcaaatgc gccaatccat atcatgctta ttttaggtgg
     8221 tattgcaatg gcattacctt ttatttggat gattttaact tcagggaaaa cgttggttga
     8281 atctacccaa attccaccta caattttccc tgagaaattt cagctaagta actatcaaca
     8341 agtacttgat ttattaccat tcatggcttt ttatatcaat acatttttaa tggttgttgg
     8401 acgagtgatt ggctcggttt tattttcagc aatggctgca tatgcttgtg cgcgtctaag
     8461 ttttccaggg aaaaatatcc ttttcagctt agtacttatt caaatgatga ttcccggttc
     8521 catttttatt actccacagt atttattagc acaaaaattg ggtattttaa atacaattcc
     8581 agcattaata ttaccagggg tagttagtgc gtttggtacg tttttattaa gacaattttt
     8641 cttaggttta cctgatgaat tggaggaagc ggcctattta gaaggtgcca atatttggac
     8701 gatctttttc aaaattatgt tgccgcttgc taaatcagga ttggttgcac ttggaatatt
     8761 cacagcgttg tttgcattta aagatttaat gtggccattg attgtcaata cttcaattga
     8821 taagatgcct ttagctccgg gattagcaaa cttacaagga caatatgcca cgaacttccc
     8881 acaattaatg gcaggttcgt tattagcaat ttggccaata gttgtgatat tcatcgtttt
     8941 ccaaagaaaa ttcattgaag gaattgctac aacaggtagt aaattataaa actattaatt
     9001 agtgattgga gagtaagtac atgacaaaaa ttacatttat tggtgcagga agtactattt
     9061 ttgcaaaaaa cgtattaggc gatgcgatgt taactccaag tctacaagat gcacatattg
     9121 cattgtacga tattgatgaa aaacgactaa aagaatcaaa tttaatgctt aaaaatatta
     9181 ataaaaattc gaatgaaaat cgtgctgata ttgtttctta ttatgatcgc aaagaagcgt
     9241 taagagaggc tgattttgtt atcaatgcta ttcaagtggg tggttataaa ccttcaacag
     9301 tgattgattt tgatattcct aaaaaatatg ggttgcgaca aactattggt gacaccatgg
     9361 ggattggtgg gatttttaga accctacgga cactaccagt aatgttagac tttgctaaag
     9421 aaatggaaga agtttgtcca aatgcttggt tattgaacta tacaaatccg atggcgatgt
     9481 taaccatggg tgttttaaaa gcgacgagta ttaagactgt tggtttatgt catagcgttc
     9541 aagtatgcgt accagaattg tttgagcatt taggaatagc cgatcaatat gacttaaatg
     9601 actttcaatg gaaaattgca ggcattaatc atatggcatt cttgttggaa attacaaaaa
     9661 atggggaaga tttctatcca acaattcgtc agttagctgc agaaaaacca aatccacatc
     9721 cagattctgt acgttttgaa ttgttgaaac gttttggtta ctatattaca gaatcaagtg
     9781 aacacaatgc agaataccat ccatacttta ttaaatcaaa atacccagaa ttgattgacg
     9841 aattagggat tccgttggat gaatacttac gtcgttgtga acaacaaatt aatgattggg
     9901 aaagcatgcg agaggacatt gtaaataatg gggagttaac acatacgaga agccgtgaat
     9961 atgcttcata tatcatggat tctattacga caggaactcc gtcagtgatt gcaggaaatg
    10021 tcttaaataa aggcttaatc acaaacttac cgacagatgt ttgtgtggaa gtaccttgtt
    10081 tagtcgataa aaatggcgtg caaccaacct atgtcggtga attgccaaca caattagctg
    10141 ctttaaatcg gacgaatatc aatgttcaag aactgacagt agaagccgct ttaacactag
    10201 ataaatcaaa aatttatcaa gcagcgtatt tagatccaca tctatcatct gaattgtctt
    10261 taacagatat caaaaattta gtggatgatt taatcgaagc acatggggac tatttacctg
    10321 aatataatta aaaagaagct gaaagagtaa ggaattttca attccttact cttttcttag
    10381 ttaaatttag taaaaatatt gacaaaacac ttagtaaaca gttacattag tgttaacaaa
    10441 aagaaaacgc attcaaattg tataaggagg agcataaaat gaagtggtct aaaaaaatta
    10501 ttttagcttg tatagcacta ggagcaacga tctctttgac tgcttgtgga aaaaatcagg
    10561 aagatgcaag cggtaaggta gaaatagagt tttttaatca aaaaaaagaa atgactcaaa
    10621 cgattcagga gattgctaaa gattttgaag caaaaaaccc tgatattcat gtaaaagttg
    10681 tggatgtacc gaatgctggt gaagtaatta aaacacgtat gcttgcaggc gatgtaccag
    10741 atgtcattaa tctttatcct caaagtatag aattgaaaga atgggcgaaa gctggttatt
    10801 tagaggactt aaccgaagaa ccgtatttag agaatatcaa aaatggatat gcacaacgtt
    10861 ttgctattga ggatcgtgta tatagcatac cattaaccgc taatgtctat ggtttttact
    10921 ataacaaaac tgcttttgag gaaatgggga ttcaagcacc agaaacttgg gcagaatttg
    10981 aaaaaatagt agctgagatt aaagatcaaa acaaagtacc gtttgcgatt gcaggagcag
    11041 aaggctggac tttaaatggt tatcaccaac tagctatagc gactgctgct ggtggtgaag
    11101 aggaagcgaa caatgtttgg cgtttctcag aagttaatgg aattaatgct gaaagcaaag
    11161 aaatgcaaaa agattttaat cgattagatt tgttaagaga accaaaagct ttgcaaaaca
    11221 actggcaagg tgccggttat aacgatacag tagttacttt tacaaaaggt gatgcgttga
    11281 ttatgccgaa tggttcatgg gcaatgccga tgattcattc acaaaatcca gattttgaag
    11341 taggaacttt tccttttcca gcagatgaag caggtcaaag tctaacgatt ggcgcaggag
    11401 acctagcctt atcagtttct gcaacttctc aacataaaga agaagccaaa cgttttgtag
    11461 aatatatgac aacacctgaa gcgatgcaga aatattatga tgtagatggt gcaccgtgtg
    11521 ctgttgaggg cgttatcgag aatactcagg attcaccatt aagtggttta actgaacttg
    11581 cttttactga tcgtcattta gtttggttag ccaaagattg gaatagtgaa aatgattttt
    11641 ataccttaac aacaaattat ttacacaatg gcaatcaaaa agccatgatt aacgcattaa
    11701 atgctttctt taatccaatg aaagctgacg gaaggtagga aaataaaatg aaaataaaag
    11761 aaattttaaa tcgttactgg gcaatcattt ttgtagcaat tccaattatt ttgcagatga
    11821 tttttttcta ctttccaatg gttcaaggtg ctttttacag tttaactaat tggactggtc
    11881 tgacttataa ttttgatttt gtgggtttga acaattataa gattcttttt tctgatccga
    11941 agtttatcaa atcgattggc ttcactttaa ttgtgacgct ttgcatgatt gctggacaaa
    12001 ttggttttgg tattttgatt gcgcgggcgt tgaacgcaaa tattaaaggg aaaacattct
    12061 ttagagcatg gttctttttt ccagcggtct tatcgggcgt ggttgtttct ttagtattta
    12121 aacaaatttt taactatggt ttaccaaaaa ttggtagtat gttaggcatt gaatttttac
    12181 aaaccagtct tttaggaaca acatggggag cgatttttgc agtaattttt gttttacttt
    12241 ggcaaggaat tgccatgcca gtcatcattt tcttagcggg ttttcaaagt attccacaag
    12301 aaattattga agctgctaga atggatgggg cgaatgataa gcaaattttt tggaatattg
    12361 aaatcaccta cttattacca agtatttcaa tggtatttat tatggcatta aaaggtggct
    12421 taacagcatt tgatcaaatt tttgctttaa ctggtggtgg accaagcgat gccacaacat
    12481 ctttggggtt acttgtttat aattatgctt ttaaaaacaa tcagtttggt tatgcgaatg
    12541 ctattgcttt agtcttattt atcttaattg ggattgtttc actggcacaa ttaaaattat
    12601 cgaaaaaatt tgaaatctag gaggtttccc aatgaaaaca tcaaaagaaa aaaatatttc
    12661 taaatacttg cttttattat tagggggctt catgatttta gttcccttag tttcaacgct
    12721 attcggttca tttaaaacaa cacccgatat tatgaataat ttctttcgtt ttcctaatcc
    12781 agcgactttg gaaaatttcc gaagattgtt agcggatgga attggtcatt acttttggaa
    12841 ttcggcaatc atcacaatca ttgcagtagt tgctgtgaca gcaattattc ctatggctgc
    12901 ttattcacta gcaagaaaaa tgactcgtgg gaaagcatac ggattgatgt atttattttt
    12961 aattttagga atttttgttc ctttccaagt aattatgatt ccgattaccg ttatgatgag
    13021 taaaattggt ttagcaaata tgtggggatt aatcttactc tatctcactt atgccgtacc
    13081 acagacacta tttttatatg ttggttttat taaactaaat gtgcctatca gtctggatga
    13141 ggctgctgaa attgatgggg ctagtcactt tacaacttat ttcaaaattg ttttcccaat
    13201 gttaaaacca atgcatgcga cgactattat tcttaatgct ctatggtttt ggaatgattt
    13261 catgctacca ttattaattt taaatcgtga ttctaatatg tggacactac cattgtttca
    13321 atataattat acgggacaat attttaacga ctatggtcca agttttgctt cctatacagt
    13381 aggaattgtc acgattacac tagtttatct tgctttccaa agaagtatta tagctggaat
    13441 gagcaatgga gcagtgaaat aaccaataaa ttattctcat tataaaggag ttcataaact
    13501 tatgattata tttgacaatc acacacaaac atttcattta caaaataaaa aaattagcta
    13561 tctattagga atcgaagaaa ataattattt aacgcatctt tattttggaa aaaagattac
    13621 gcactattct ggtgggtatc gctacccccg aacagatcgt tccttctcac ccaacaatgc
    13681 aaatgcgcaa gatcgtttat tatctttaga cacattacca ttagaatttc caggatatgg
    13741 acatggtgat tttagagaac cggcgcataa tattaaatta ggaaacggta gtcgtgtaaa
    13801 tgattttcgt tatgatagct acgaaattca aaaaggaaag cccagcttag cgggattgcc
    13861 agccttatat acggaagatg atgatgaagc tgaaacgctg attattcaat tgaaagacgc
    13921 gattagtgga ttgactttac agttgttcta cacaatttat caaaatcaca gtgtaattat
    13981 tcgttctacg caattagtaa ataatagtga tgaaacagtg gagattaacc gcttagctag
    14041 tcaatcaatt gattttccaa atcgtccact cgagttaatt catttaaatg gtacctgggc
    14101 aagagaacgt caaatgacta gagaaaaaat tcatacaggg ataaaagtat tagatagtaa
    14161 acgcggatca agcagccatc aacaaaatcc gtttgttgct ttagtagatg ccaaaacaga
    14221 tgaatttcaa ggagaggcat atggatttaa tttagtttat agcggtaatc acgaaacggt
    14281 gattcaaaaa gatccatttg accaaacacg ggtcattatg ggaattaatt catttaattt
    14341 tggttggcaa ctaaaaactg gcgactcatt tcaaacgcca gaagtcgtga tggtttatag
    14401 taatgaaggt ctgaatacgt tatcacagac gtatcatgaa ttgtataatc accgtttaat
    14461 tcgtggaaac tatcgtttaa aagaacgtcc cacgttaatc aacaactggg aagcaacgta
    14521 ttttgatttt aacgaagaaa aaatcatgaa tattgtcgat gatgcgaaga acttgggcat
    14581 tgaaatgttt gttttagatg atggttggtt tggcggtcgt gaaaatgact tcacttcatt
    14641 aggtgattgg gttgaaacag aaggcaaact tgcttgtggt ttggaaaatt tagccaagca
    14701 agtgcatgat aacgggatga aatttgggat ttggtttgaa ccggaaatga tttctgaaga
    14761 tagtgaacta tttcgagcac atccagattg ggcattgggt attccaggtc gtgaccgctc
    14821 aattagtcga aatcaatatg ttcttgattt ttcaagacaa gatgttcgtg ataatatcta
    14881 tcaacaaatg acggcgattt ttgatcgtgt accgattgat tatgtgaagt gggatatgaa
    14941 tcgaaatatg acagaagtct attctgtctt gttggactca acgatgcaag gtgaagtggc
    15001 tcatcgttat atgttagggt tatatgaatt tttagaaaag ttaacaacga attatccaac
    15061 tattttattt gaaagttgtt cgggtggagg cggtcgtttt gatgcaggtt tcttgtatta
    15121 catgccacaa acttggacga gcgacaatac cgatgccgtg gctcgtctga aaattcaata
    15181 tggaaccagc ttagtgtatc caatttcaag tatgggtgcg catgtttcag cagtcccaaa
    15241 tcatcaaaca ggaagagaaa cgagtttaac tattcgtggc aatgctgcga tggctggtgt
    15301 ttttggttat gagctagatc taacgcaatt aacttctgaa gaaaaagcag aaattaaaga
    15361 acaagttgcc ttttataaaa agcatcgcca acttttgcaa tatggaactt tttatcgctt
    15421 ggaaagtccg tttgaaggca atgatactgc atggatgttt gtctcaaaag accaaacaga
    15481 agccatggtc ttttatttcc gagtattagc agaagctgcg catccattag tcacattgaa
    15541 attagcagga ttagcagaag aagcaactta tcaaatggaa gatgtaacat ttgctggcga
    15601 tgaattgatg aatctcggtt tttatattga ctcagaacta catggtgatt atgcgacaca
    15661 acgcttttat ttgaaacaga tatagtagct tgaatttaca atataagtgc aacaccaaaa
    15721 gagactcatt ccaaacgaag aaaatatgtt ttatttacac gcatttacta aaaagcagcc
    15781 tgatttgaat tgggaaaatg aagaagtacg tgaagaaatc tatgcaatga ttaactattg
    15841 gttagataaa ggactaggcg gatttagaat cgatgcgata ttaaacatca aaaaaaaact
    15901 agagtatggc atctttgaat ctgatggaga agatgggtta gccttcattg ggccctggat
    15961 tcttaatcag tcgggcattg aagtgtggtt aaaagaaatg agagagcgga catttaaacc
    16021 acataatagt atgacggtag cagaagccga tgttcctaat gaacgtttag atgaatatat
    16081 tggtgaagat ggctattatt cgatggtttt tgattttagt tatacggata ttgatgtacc
    16141 tgaaactggt gaatggttta aagatagcca atggacatgg acggatatgc gtaccaatat
    16201 ttttacgaat caattagtta cgcaagataa aggttggggc gccttatatt tagaaaacca
    16261 cgatcaacca cgttcaatta ataaatatat tccagaagaa tggatcaatg attatagtaa
    16321 aaaaatgttg gcgacgcttt ttatgttact aagaggtacc ccttttattt atcaaggaca
    16381 agaactagga atgaccaaca tagaaatggc atcattagaa gattttgatg atgtagctac
    16441 gcattctcag tataaacggg cattagaata tggattggca ccagagcaag cattgaaagc
    16501 tgtcaataaa cgaagtcggg ataattctcg cacacctatg caatggaata cgcaaaaaaa
    16561 tgccagattc tcaacttctg ataatgtatg gttaaaagta aatcctaatt atccagaact
    16621 caatgcagaa gctcagttaa ccaatcaaca gtcggtattc aattattatc atcaattaat
    16681 taaattaaga aaaaatagtg tatacaaaga cgtgctaatc tacggacaat ttctaccagt
    16741 gaaagataaa aatgaacata ttttgcttta tgaacgccaa ttagaagata aaatcgtatt
    16801 agttatctta aacgttacag caacagaaca agaatattat gtagatgaac agtaccaaca
    16861 agtattaatc aataattatg aagatgtttt attagatcaa agtcgaaaat tgcgtttacg
    16921 accgtttgaa agtattgtat tagctaatta tggaggacaa ttatgaaaat taaaaacgaa
    16981 gcaatgctca tcacctatgc agatagttta ggaaaaaaca tgggcgaatt aaaacaagtc
    17041 ttagacaaac atttacaagg tgttattggt ggtgtacatc tgttgccttt ttttccatcg
    17101 acaggtgatc gaggctttgc gccgagtgat tatacgacag ttgatccagc tcttggaaca
    17161 tgggaagatg tggaagcatt aggtgaagat tattatttga tgtttgattt tatgattaat
    17221 cacatttctc gtgaatcaaa atttttccaa gatttcaaaa aaaaccatga gcagtcacca
    17281 tacaaagaaa tgtttatccg tattcatgaa tttttcccag aaaatcgacc aacacaagaa
    17341 gatattgatt taatctacaa gcgtaaagac aaagcgccgt ttcaaacggt agaatttgcg
    17401 gatggtacga cagaagaggt ttggaatacc tttggggaag aacaaattga tttagatgtg
    17461 accaaagaag ttgtgaaaga atttattcga gaaaccatca aagatatggc gaaccatggc
    17521 tgttctttaa ttcgattaga tgcgtttgct tatgcgatta aaaaattaga taccaatgat
    17581 ttcttcgtgg aaccagatat ttgggaactt ttagacgaag tgcgtcaaga agcagcgaaa
    17641 tatgacgtcg aattattacc agaaatccat gagcattatt ccattcaaat gaaaattgcc
    17701 aatcatgatt attatatcta tgacttcgcc ttgccaatgg tcactctgta ttcactttat
    17761 agtggaaaat cagaacgttt agccaattgg ttgaaaatga gtccgatgaa gcagtttaca
    17821 acattggata cgcatgatgg aattggtgtt gtggacgctc gtgatttatt aacagatgaa
    17881 gaattggatt acacctctga ggaattgtat aaagtcgggg cgaatgtgaa gaaggtctat
    17941 tcaagtgcca attacaataa tttagatatt tatcaaatta acagcaccta ttatagtgca
    18001 ttaggcgata atgaccgtag ctatctgtta gcacgtgcga ttcaagtatt tgcgccaggg
    18061 attccacaaa tttattacgt tgggttatta gcaggaaaaa atgatattga actgttggaa
    18121 gcaacgaaag aagggcgtaa tattaatcgt cattactatg atttagccga aattgaagaa
    18181 gaagtgcaac ggccagtgat tcaacaatta tttgcacttt taaaatttag aaatgaatct
    18241 gcggcgtttg atttggatgg aacgattgaa gtagcaacgc catcagaatc agaaatagtg
    18301 attacacgta aaaataaaaa cggtagtgat agtgcaacat taacggctaa cttggatacg
    18361 aaagaattta tcattgagtc taaagatata aaaattttca ctagtgttgc attaaaattt
    18421 taacctattc catagaacaa aaaatccttt atactagcac ttggatgact tatccaagac
    18481 caataaaaag gactcataca tggataagta taaaacaaat acagcattta ataaatggtt
    18541 ttctgccata aaattaggaa aactttctgt caactcccaa aaatgtattt ttgattacaa
    18601 ttcgtatcgt aaaaaactgt cttttgaagc tgtgctcaag ctctttctct acgcagttaa
    18661 tgaagaaaaa gaaagtttgc gtgatttgag tacagctctt atcgaccgat cgctccaact
    18721 agaaaccgat gtgtcatcga ttagtcatac ccaattgtct cgagcattta atgcacttga
    18781 acctaaagtg ttagaagaaa tttttcaaca actgttagaa aaagttcaac atcaaatcaa
    18841 accaaccaag cgaaatcatc tgtatctgat tgattccagt acgttttcgc ttagtttaaa
    18901 acgtcataag tgggcgactt tccgtcaaac aaaatcaggt gtcaagttac acttaaacta
    18961 ttgttatatg gacgatacta cgatgtatcc aactgatttt accttaacaa atgcccatga
    19021 acatgatctc aatcaactac cagtattggt gaatcaacca gaggcaactt acgtgtttga
    19081 tcgtgggtat taggactttg aaaagatgga tcagatgcat tgggatggct atttcttcgt
    19141 cactcggatt aaaaaaaaca ccaaagttca cgtgatagat actctggaaa catcgccaga
    19201 aacagaaatt ctacgagacg agctggttcg tttaggatct aaaacctatc taaccgccaa
    19261 tttccgacta gtcactgttc aagataagaa cggacgtgtg tttcaattta tcaccaatcg
    19321 aatggacgtc tcttcaaaag aaatctctga tatgtatcac gctcgttggc aaatcgaatt
    19381 gtttttcaag cacatcaaac aacacatgac cattaaaacc ttcttttctc aaagtgaaaa
    19441 aggcgtgcag aatcaattga ttttaacgat gatttccgct ctcctcacgt ttctcatcaa
    19501 attggagacg aaaacggaaa aatcagtgtt ccaaatcaaa cgattctttc gttatttact
    19561 gtttcagccc tttgaatgtt gtctagaaaa attaattccc acatgagaaa aaagaaattt
    19621 ggctcgttac ttttggtttt cagttgaata acagataaaa aattttctgg aaaaagtcat
    19681 cctctgcgtc agcacagttt tttttgactt attttcagag gaaatagttt tcagaatatt
    19741 ctaaaatgtt ttatgcaaca ctagtgatat tttttagaat tagatttcta atgtgcattt
    19801 tttgagtatt aaggagcaag tatacaaaga tttataaagc tgattaaatt tgaataaaaa
    19861 tatgcaggaa cgctactatt tttagtagtg ttctttttta tatttgaata aaaaatgtgg
    19921 tggttaaatg gtggttataa caaaaaatga attaatgaaa ttaggttatg ggaagtatca
    19981 gtctgagaga atcatacgac aggcaaaagc aattatggtt tcaaaaagct atacctatta
    20041 cgaaaataga agattaggac tagtacctgt tgaatctgta gaagaagttt taggtatgaa
    20101 gatagaggta gaggaggatt ttcagaatca tggttaattt taaaaaagat aaaaatggga
    20161 aatatcattt tactgctagt ttgggattcg acaaacgaac agggaaaaga gtacaaaaaa
    20221 acgtagaggg gttaaaacag ttaaagaaga aaaaaagcgt ataccgaact tgtttctaat
    20281 tatggaaaag aagcagaaac ttctcaaagt actatgttgt ttgaggagtt tttttataaa
    20341 ttattcctac cttattatca agggaaagta gaagaaagaa catataaaaa tcggttatct
    20401 acaatcaaaa aaacttttct tatttcttta aaatggagtt aagagaaata cgtccaattg
    20461 acattcaaaa gtggcaaaat aatttattga gcaaatataa aaattcttat gtaagaaaca
    20521 tgtatgggat ttttcagatg tcattagatc gagcagttgt tttgggacta cttgaaaaaa
    20581 atcctgcaaa agttattgga aatgtgaaga aaaggaaaac tacagttgat ttttggacaa
    20641 aagaagaatt tacaaaagtt atttcaactt tttatattga tgatttgtac caacattttg
    20701 gttttatttg tatgtggtta ctttttatga ccgttatgcg tttgggagaa gccactgcat
    20761 taacttggaa agatattgat tttgaaaata aaaacttgcg tgtgaataaa acactctatt
    20821 ataagagagc caaaacctat gagtttgtag aacccgaaac ggcttctagt attcgtacaa
    20881 tcgaactaga cgacaataca attacctttt taaaagcttg gaaggagcgt caagtttcaa
    20941 taggaggcat gtcgtttgta ctttcttata atggaacacc tactcagaaa tatacaatcc
    21001 gtcatattat gagaagacat gcaacgatcg ttggtgttca tataatccgc atacacggat
    21061 taaggcatag tcatgcttct ttgcttatat cacaataaaa agatgcactt cgaattaaag
    21121 aaaggttaaa acatgctgat gtacaaacga ctttaggaac ttatggtcat ttgtatctaa
    21181 attcattgag agagataaca aatgatccca cagattatat ccaaactaat ttttctaaaa
    21241 tcaaaccgac acctatttac gataataaaa aataaatgat aaacaaaagt aggatcacac
    21301 caaaagaaac acactagaaa aactataata caggcttttc tagcgtgttg tttcttattt
    21361 ccgcctattt atacattata ctcataaagc atttaatgca tcgatcgtag ccgcattgtc
    21421 tatatgggta tataaagttg ttgtattggt acttgaatgt cccaattgtt gggcagttaa
    21481 taccaatgaa tttgtctgtt ggtagagtct agtagctaat gtatgacgca gtttgtgagg
    21541 gctgacttga attctaaagc cttttgaata ttttgctacc atccgttcga ttgcaatacc
    21601 ggatatacgc ttggcttctc cttggtaaat tgctagaaat aaagcttttt cgttttctgt
    21661 tactttatat cgttctttcc gaatagctag ataattagct aaatattcca ttgcgaatgc
    21721 tgctatattg acagcatctc ttttatttcc ttttctagtt actaccacat tattcttatc
    21781 taaagatagg tcatctaaat taatatttat aacctcggag agtctcaatc cagatcctaa
    21841 aatcagtgca ttgatcgcca ggtctctttc tacatctcgt tgataataga tcaaagctcg
    21901 tttactcaaa gtatttttat attcattggc aatataatct agataatttt gtgtatcctt
    21961 atctagaaat aattttttct caatggcatt ggcacgtgca gctaacgtag ttttatctgt
    22021 gatggttgct acttgatcca taggattatt gtctaagtat ggttttccta gaggattatt
    22081 ggaacttttc gaaagatatt taaatagaac ctttaatgcc gtgatatttc tattgatagt
    22141 agaagtagat agacctaagt tattattatc tttgagctta gtttttgttg tctctttttt
    22201 tacattttct tttgtgcgac gtttcaaatg agatttgtac aagtttaggt cataggcagt
    22261 taactgtctt atcgaggtga tagaagtatc aggaacgata gactcactca gaagccattc
    22321 ataaaagagc ttataatttt ttagatattc taaagtggtt cgaggtgatt gatctttatc
    22381 ctcacgatct tgaacaaaat cttttatata atcgggcata tgaagtatga ttgtatctat
    22441 tttttgatat atttctgtat ttttcatgtc attcatcctt tcaataagta ggggagaaga
    22501 gtatctctat aattggacta taccaattat agaatgtttt gtcaattggt atgctctaaa
    22561 atttattaat ttgtttcaaa aaaagcatat aaacaggtat acgtttaata atttaaaata
    22621 aatggtatac ataaataggg aaggaaatag aagggagcaa tctaaatttt ttgaattttc
    22681 ttaattaatt atatagaaag aacaatcttt ctatagagtt agacatgaga gggactatgc
    22741 ttgatttaaa atcaacaagc gtatatactg tatatacaaa gaaaaaggag ggagaagact
    22801 atggcaatta aagaaagaaa atttagggaa gtggggaact cagtagtatt aactatttca
    22861 aaggagtttc ttaaagaaaa tgggttaaat cccggagata cggttttttt agatgaagaa
    22921 aaacttcgag cggcaattac aaaaaaacaa gccttatctg atgaaattga tttagcaata
    22981 tctcaagcat taactgaata tgatactact tttagagagc ttgtcgataa atgagtattc
    23041 attacctaga agccaaagat attataaaaa tgaatgtaat ccaaataaaa aaatactctc
    23101 ctaatgaacc ccttggtgta aaggatgcga atgctttaga aatgtgtgta gggcaacttc
    23161 gagcctcagc ttttggtgaa gaggtttatc cttcgattta tgaaaaagca gctatcctac
    23221 ttatacaatt gataaagaaa catccttttc acaatgcaaa taaacgtact gctttcttag
    23281 ctacttttgt ctttttgaaa atcaatggac agcttttagc tattgatcaa aaagatgctg
    23341 taaatttagc ggtatatatt gctacgtatg ataaggattt cgatcaacta aaatatgaag
    23401 tgatcaacac tataaagaca catacgcagc ccatttaagg agttactgta tgtgtcttta
    23461 tagggttatt agctgctata gcgaaata
//
LOCUS       DARBDV010000039        23066 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000039 DARBDV010000000
VERSION     DARBDV010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.039"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(110..565)
                     /locus_tag="SCM56_001450"
     CDS             complement(110..565)
                     /locus_tag="SCM56_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110234.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(719..1927)
                     /locus_tag="SCM56_001451"
     CDS             complement(719..1927)
                     /locus_tag="SCM56_001451"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEG1110235.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2039..2371)
                     /locus_tag="SCM56_001452"
     CDS             complement(2039..2371)
                     /locus_tag="SCM56_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEG1110236.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2519..3394
                     /locus_tag="SCM56_001453"
     CDS             2519..3394
                     /locus_tag="SCM56_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG1110237.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3476..4057)
                     /locus_tag="SCM56_001454"
     CDS             complement(3476..4057)
                     /locus_tag="SCM56_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HEG1110238.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4063..5322)
                     /locus_tag="SCM56_001455"
     CDS             complement(4063..5322)
                     /locus_tag="SCM56_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEG1110239.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5324..6370)
                     /locus_tag="SCM56_001456"
     CDS             complement(5324..6370)
                     /locus_tag="SCM56_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110240.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6605..6880)
                     /locus_tag="SCM56_001457"
     CDS             complement(6605..6880)
                     /locus_tag="SCM56_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEG1110241.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7123..8433)
                     /gene="der"
                     /locus_tag="SCM56_001458"
     CDS             complement(7123..8433)
                     /gene="der"
                     /locus_tag="SCM56_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEG1110242.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8615..9844)
                     /gene="rpsA"
                     /locus_tag="SCM56_001459"
     CDS             complement(8615..9844)
                     /gene="rpsA"
                     /locus_tag="SCM56_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEG1110243.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9979..10659)
                     /locus_tag="SCM56_001460"
     CDS             complement(9979..10659)
                     /locus_tag="SCM56_001460"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEG1110244.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10708..11343)
                     /locus_tag="SCM56_001461"
     CDS             complement(10708..11343)
                     /locus_tag="SCM56_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG1110245.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11409..12812)
                     /locus_tag="SCM56_001462"
     CDS             complement(11409..12812)
                     /locus_tag="SCM56_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEG1110246.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12799..13830)
                     /locus_tag="SCM56_001463"
     CDS             complement(12799..13830)
                     /locus_tag="SCM56_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1110247.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13861..14082
                     /locus_tag="SCM56_001464"
     CDS             13861..14082
                     /locus_tag="SCM56_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEG1110248.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14247..14984)
                     /locus_tag="SCM56_001465"
     CDS             complement(14247..14984)
                     /locus_tag="SCM56_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HEG1110249.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15137..16477)
                     /locus_tag="SCM56_001466"
     CDS             complement(15137..16477)
                     /locus_tag="SCM56_001466"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HEG1110250.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16714..18237)
                     /locus_tag="SCM56_001467"
     CDS             complement(16714..18237)
                     /locus_tag="SCM56_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEG1110251.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18615..19226)
                     /locus_tag="SCM56_001468"
     CDS             complement(18615..19226)
                     /locus_tag="SCM56_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEG1110252.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19349..19474)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19556..20272)
                     /locus_tag="SCM56_001469"
     CDS             complement(19556..20272)
                     /locus_tag="SCM56_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEG1110253.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20278..20847)
                     /gene="scpB"
                     /locus_tag="SCM56_001470"
     CDS             complement(20278..20847)
                     /gene="scpB"
                     /locus_tag="SCM56_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEG1110254.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20831..21628)
                     /locus_tag="SCM56_001471"
     CDS             complement(20831..21628)
                     /locus_tag="SCM56_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEG1110255.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21598..21969)
                     /locus_tag="SCM56_001472"
     CDS             complement(21598..21969)
                     /locus_tag="SCM56_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEG1110256.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21988..22875)
                     /gene="xerD"
                     /locus_tag="SCM56_001473"
     CDS             complement(21988..22875)
                     /gene="xerD"
                     /locus_tag="SCM56_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEG1110257.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6469 a         4926 c         3910 g         7761 t
ORIGIN      
        1 aaaatcttgt cacagtccct tattctgaat aaacggtgtg gaagagacag cctatcagta
       61 gtaataagac acatctcttt cacagcctca tttttgcgta aaaatcgatt tattcgaaaa
      121 attccccgta tacatcttct acacgttgat ctggattgat cacgatatac aagttacctg
      181 ttttttcgct gatttttgtt gattgatatt gattgtctaa tccttctgtt cctttatcta
      241 gatatgggaa gtacacaaga tcaggtgtgc catttcggta aaggatttcc attcgctcga
      301 tatcttcgta tttcaatgcg cgattaaaca tgcccatttc cagtccgcct aggttgatat
      361 catgtgtcgc aactgcgtcc ccttcatgga agatttcgat tttaaatcca gcacacggat
      421 ggatttcaac aaattcactg ccattgattc gtccaaaact tgttgtgatt tgtttgatcc
      481 aaagatcgcc gatatgacgg cggttgatcg tccaggtttc tccatttcta aaataaaatt
      541 ttagcgctgt catttcttta ctcattaaaa agactccttt atgatgtaat ttattcttgt
      601 actacctcag tttagcacaa atttatgtaa gtggtcacat cttatgtttt ttcttctaat
      661 gacgtatctg cccactttaa gtagccaaca aagctgagca gatacagtct agtgtttttt
      721 aaatcaattt ttctttcgat aaaaaatcca aaacctgttt tttatcattt tttattcttt
      781 gttctacgac ggctttttca gctaattgca aagcttcact aacccactta ccaggttttt
      841 ttccagtttc ttctagcaag attttaccat ctactgctaa ttcgcttaat gacttgatag
      901 gtaaacgatc ataggattct cctacggcat ccatctgagg tgtttcgtca aaatagatca
      961 tcaattcttc tacttgcagt gcatgttttt tgcctaagaa gtacaattcg aatggttcca
     1021 aagctccagt ttctcgttgt cgtagtcctt ggacaagtgc ttgaacgtct ttaatcattt
     1081 ggttagaaca cttccacgat ttcaaaaagg aacgaatctc attttctttt agatccagtt
     1141 ggctgatcaa taatgcccat gcttggctct cttctttcaa tgggacggat ggtaattcag
     1201 caaaacgtaa aagtgcttcg ccttcttctt tcaaaccggg acagcaaatg tagcactctg
     1261 tttcaataaa tgcatggatt cccgcggccc cttcatttcc caagagcatc ttgatgaatt
     1321 ccacatttat acgttctaca gatattttac ctaatagctc gtggttttct ttgatggcgt
     1381 agaaggtttc ttcctctaat ttgaacccta gttgactgac aaaacgcaac ccgcgcatca
     1441 ttcgtaaggc atcttcatgg aaccgctcat gaggattgcc tacagctcgc aagatctgtt
     1501 cttctagatc ttgcagacca tcgaacaaat cgatgatttc tccatcttct ttcaaagcaa
     1561 acgcattgat cgtaaaatct cttcttttca aatcttctgc caacgaacga acgaattcga
     1621 catgatctgg tctacggtag tcctgataag ttgattccgt ccgaaatgtg gtgatctcat
     1681 attgttcatc attttctaat accaacacag ttccatgttc gatcccaata tctatcgttt
     1741 ttggaaaaag ctgtttgatt tcttctggaa atgcgcttgt tgcgatatcc acatcatgga
     1801 ttggctggtt cagcaagata tcacgaacac ttccacctac aaaataagct tcatatcctg
     1861 cattctctaa ttttttcaga actggaattg ccttttggta ttccagaggt aattgtttta
     1921 atttcatttt tccaaccttt cctgcgtgtt tcttgtcctg agataaagac tcagttgcat
     1981 ggtgactgtt tgacaagtcc gtttcagagt cttcaaggcc aggaccactt catcttgttt
     2041 actcgtcctt acgctcaaag cgattatggt ctctttgatt gaatttttcc atgttttttt
     2101 caaaaacttc agttaagtcg atattcaatg aattagccat gatcatcgta acgaataaaa
     2161 catcaccaag ttcttctgct accgagttaa ctggttcaga agcctttttg cttttttctc
     2221 cataagtgtg gttgacttct cttgccaatt cacctacttc ttcagttaat cgagccatct
     2281 gagctaatgg agagaaatac cccaccttga attgctggat atattcatcc acttcctgct
     2341 gcatactgta taaagaacgc tttttatcca tctttttccc tccaaaacta tcttaccaaa
     2401 actttaacgc aaatcctaga taagagcaat tgagaaaatc gaatttccat gctacattta
     2461 ggaaggaatt tctaaatgga aattctgtga aaatacataa ttgaaagctg gaattcatat
     2521 ggtcaaacga aaaacagtaa aagaagttct ttttatcatt ataggaacaa gtatttatgc
     2581 atttggtctt gtttacttga acatcgctaa tcatttagca gaagggggag tctcagggat
     2641 caccttgatc ttaagagctt tatttggtat tgatccagct tatacgactt tattaatcaa
     2701 catcccattg attttactag gaggaaagat cctcggtaaa cgttccttgg cctatactgt
     2761 tctaggtaca ctttctttgt cattcttttt atggatctgg caaagggttc cactagaaat
     2821 caatttacag catgatctac tgatcgtttc tctacttgcg ggtttagttg caggtgtagg
     2881 aagcgggatc gtctaccgca tgggtggaac aacaggcggc agtgatatta tcgctcggat
     2941 tttagaacaa aattacggca tcactatggg gcgttcccta ttggcatttg atatcatcgt
     3001 cttacttgct tcattaacct atatcgactt gaaacgtatg atgtatacgt tgattgtttc
     3061 gtatgttttc agtcgtgtga ttgattcgat cttagatggc ggatattctg caaaaggtct
     3121 acttgtcgtc tccaacaaaa gcgaagagat tgcaccacta ctgatgaccg gtctgcaaag
     3181 aggtgtgact ttcctttctg gagaaggcgc attctctggt aattcaaaaa aaatcatcta
     3241 tatggtcgtt agctctagtg aattaactga agctaagagg atcgtccacg agattgacga
     3301 aaaagctttc ctttcaatca tcaatgtgca tgaagtggaa gggcaaggct ttacttattt
     3361 gaagccacag acaagattac tgaaaaaact ataagaaaaa agacaaagat aggctatgag
     3421 gaaagattcc acatccagct gacctttgtc ttttttagta ctatactatt tttaactaat
     3481 tgttatgttc acttacccgg ttacttttct tttgtgtctg caaaaggatc tttcggttaa
     3541 cttcatcaga taatttcaaa aaagcatccc tatctccatc ttctagtgct tgatcgattt
     3601 gagcataaag catttttagt tttgcctctt cttctttttc ttgaaaatat cgttcaatta
     3661 attcagtatc ttcttcactg actgaagaat cccaagaaga aaatggattg tcttctaaga
     3721 tagatagata ttctcttgtc tgccaagcat tttcgaatat acattccaca tataaaggct
     3781 ttttccaatt caaacggatt tcatgaaaaa tctggtctgt gtccgtaaat tcttttccct
     3841 gcaaaaacaa tttcatgggt tccccttcaa cagatagatc actgatctgc agtcctcgat
     3901 ccgcaaaatt cgccccttca acaaagtgga cattgcttaa aatcgcttca tgattggcaa
     3961 gataattcaa gatccaaaaa acttctcttc ggctaaaaga tacatgattg accatccacg
     4021 ttaaaaaatt cttcttttcc cgtacatcaa ttagcatttt tctcacctct ctgttaggtc
     4081 atctaataat gacagtgcct ccatatctcc tggttcaagt gttaaataat aagtcaataa
     4141 ttcttgcgct cttttcagct gcccttcttc tcttaagaaa aaggcatact ctttcaagaa
     4201 atctggttca tgtgatagtt catgataagc ctgttcatag tgtacagctg caacggtaaa
     4261 gtcttctaat tcattataag catgggctag gttccattct gcataaggat tagcagtttc
     4321 ttccatctga ttgatcactt gtatcacatc ttcatatctt tcatcatcca agtaaagatt
     4381 acttaaagta agtaatgttt catcttgttt gtctcctact tctaaagctt tcagtaaaag
     4441 ttcttctgct cgctgtctat catgcagacg gaaagcattt tccgaggcca ggtgataaag
     4501 ttcgacttgg taaggatttt ctttgattcc ttcttctaac actgtttgtg cttcttcaat
     4561 catttcttct tcttgaagag cttgtcctag atatagatat aacgattgat aatgcggatt
     4621 aacttctcga agttcttcca gatagttgat tgcctgctga ttttctttca attgaagatt
     4681 cgtaaaagct agctggaaca gtcgatcatc tgtcctttct tctttcagtg cttcttctaa
     4741 aggtacgata gctgcttcga attctccttg catacttaag gattgtccta aacgttcctg
     4801 catcgagatt cctgaaattt catttatccc gcttgccaac aatgtttcat aaaccactgc
     4861 tgcctctttg aaacgatcaa ctgaaaaata caattcagct agcgcaaact ggatcaatgg
     4921 ttcatcagga agcaacccag ctgcttcttt caattttgct tcactcactt cagggattcc
     4981 gataacttgg tacaaatcag ctatcgccaa taagctttgc ggatagtaat cactttgctt
     5041 atccactttt tccaggtatt caaaagctaa gtcgatctca ttatcttcga ttgcaatttc
     5101 tgctaacggg atgttcagtc cgtcattttc tgggtattgg ttgatcagtt gttcaaaaat
     5161 ctgttttgct tcgatcaaaa agccaatagc cagcaattct tcgcctagtt cagctaatat
     5221 atctgctggg tcttctttta atgctttttc taaaaagatt tgtgcttgtg acaagtcttc
     5281 atctgataaa gcttgcaaca ttttttcgct atttgtattc atttcaattc cctcttatct
     5341 cactgacgat ttgttgaaat accgtcattg ttctcaataa ttctttttcg tttggatgtt
     5401 ggtatccgtc tattatacct attttttcta aaacaagtat ttttttgctt ttttctgcca
     5461 aaaaaagaac tttttccaaa tactccatct ttgaaatctg ttccagccag tggattggat
     5521 agccaagcaa gtccagccac acaaaaaagt tttttagttg gaaatcaaaa ggatatgctt
     5581 ggagattcca cacgaaatga aacatcattc ctaaaaattt tttcatacta gcagaaagta
     5641 gatggccatt ttcggtcaga taaaaagctt gttcaaacag tttaccataa tttgacaggt
     5701 tttctgcctc taattgataa aaatagatca tttcttcgat catcccggca aacggccgag
     5761 tcgttaattg ctttttattt ggatagtttt tatacagatt ttgcaaaaaa cgatgatcac
     5821 aaatcattcc gcaacagata aaagtcaaaa agtcaatctg ctttccgtcc acctgccgct
     5881 cgctgattgt ttgatcataa acgatgcgtt ctggcagatt gatggtttgc agcactacac
     5941 gatttggtag ctgcaaaatc gtccgctgcg gtgttaatgc tcggcttaga gaacggatcg
     6001 atacagggat catccatagt gatgaatcaa gggttgtatg tttttgaaaa aaacctgcca
     6061 gatccatgat cccttcattt ccaaatccga tgataaacat ggattgattt tcgggatagc
     6121 gtttgacgaa atcaagcagg ttctttaatt ctgttaaatg attgcattga gtgttggcac
     6181 aaatatacca gtcattgttt gcttgatttg caaataacgg gttgatctta tctgcaaaca
     6241 aatcataata gcgctgattc gttaaaaata agattggctg atcaatggaa agttctctta
     6301 gctgactgac tagcgtttct ccgtaaaaca ctttcgtcaa cgcatcttct acttgataat
     6361 caagttccat acgcatccct cctcttctta tttttatttt accattagta agcaaattct
     6421 agaaaagata agagcaaagc tcacctcata acttggaaat aaataaaaat taaataaaat
     6481 acatgtgttt tctatgtatg acatacggat tcggaatctc tgtctcttat ttattccttt
     6541 tctgctagga aataaaaaga aaagcacttg tagccctaga gccacaagcg ctttttcaac
     6601 tgtcttattt aacagcgtct tttaatgctt tacctggttt gaatgcaggt actttgctag
     6661 ctggaatttc gatttcttca ccagtttgtg ggttacgacc tttgcgagct gcgcgttcgc
     6721 gaacttcaaa gttaccaaaa ccgattaatt gaactttttc gccgttagct aaagcatctt
     6781 gaatagttga aaatacagca tctactgctg cagttgcatc ttttttagtt aaatcagtag
     6841 ctgaagcaac tttttcgatt aattctgctt tgtttgccat ggatagattc acctcctcca
     6901 caagagatat gatagaaata tcatttagtt aaaactttct gagtggtcca gaagtttaaa
     6961 aagaaatatt gattaacttc aatattctgt tatcacgata tcatatgaac cgcttaatag
     7021 caaggttttt accaattttc acatgctttt tttagtcata gaaacgtaac aaacgaaact
     7081 gtctttttct tttgaaaaaa aaacgtcgat atggtaaaat gcctattttc tcctacgagg
     7141 gataattcgg atggacgtcc cttcaaaagt aaatgctttt ctgatttgat tttctaagaa
     7201 acgttcataa gaaaaatgca tcaattcctc ttcattgaca aaaacgacaa atgtaggtgg
     7261 tttgacagcg acttgtgtag cataaaaaat cttcaggcgt tttcctttat ctgttggtgt
     7321 tggattgatt gctacagcat ccataatgat atcattcaac acagccgaag agatacgaag
     7381 attctggttc atacttactt gttcgatcat ttcaggcaga cgttccaacc gttgtttagt
     7441 cactgcagaa acaaaaacga ttggagcgta atcaagatac cggaattcat cacgaatctc
     7501 ttgttcgaaa tcacgcattg tattggtttc tttctttaca agatcccatt tattcacgac
     7561 gataataatt ccacgaccag cttcatgggc gtaacccgct actcgcttgt cttgttcccg
     7621 aatcccttct tcagcattca gtaccatcaa aacgatatca gaacggtcaa tggcacgcat
     7681 tgcacgcatc acgctgtatt tttcagtatt ttcgtatact tttccgcgtt tacgcatacc
     7741 agctgtatct atcattaaga atttctgacc actttcagaa acgaagtggg tgtcgattgc
     7801 gtctcgggtc gttccttcaa tatccgaaac aattacacgt tcttctccta aaatcgcatt
     7861 gatcaaagag gatttaccaa catttgggcg accaatcaaa ctaaacttaa tcgtgtcgtc
     7921 gtcctcttct tcagtctctt cagagaaata cttcactgct tcatctaaaa tatcaccaat
     7981 ccctagtcca tggcttcctg aaacaggata aggatcgcct agccctaagg aataaaattc
     8041 atagatatcg tttctcattt ctggattatc tactttattg actgctaaaa tcactggttt
     8101 attacttctg tataaaattt tcgcaaccat ctcatctgcg tctgtcacgc cttcgcgtcc
     8161 gctgactaca caaatgatta catctgcttc ttcaatcgcg atttctgctt gatgcttgat
     8221 ctgatccata aaaggttcat cgccaagatc aattccgcct gtgtcgatca cgctgaattc
     8281 tcgacctaac cattcacctt ttgcataaat acgatcacga gttactccag gagtatcttc
     8341 gacaatcgag atacgctcac cagcgatgcg gttaaaaatc gtagacttac cgacgttcgg
     8401 gcggccaacg attgcaattg ttggatttgc catattattt cctcctcaca agtttccaat
     8461 ctcacttagt ttaacgaatg cattgccaac tttcaagcat agattgtcaa ttaaacgaag
     8521 tatttttatt ccttattgaa atactaatcg cttttcaaaa gaaaaactga gacaaaagca
     8581 gctgctttca tctcagtcac agatacaaaa tcaattattt ttcagaatca tcggttgctt
     8641 tttcttctac ttcttctttc aacgcatcac ctaaaatgtc tcccatagta aatccagtat
     8701 tttcttctgg taattcataa gactcaacat tttttggttc ttcttctgaa gctggttttt
     8761 cttctaaagc tttgatactc aatgcaatac gatgttcttc tggatggatc tctaatactt
     8821 tcactttcac ttcgtcacct tcatgaagca cttcatgtgg tgtcgcaata tgtttatgtg
     8881 aaatttgaga gatatggact agcccttcta ctccagggaa cacttcaaca aaggcaccga
     8941 agcttgtcaa acgtttaact gttccgtcta atactgtgcc aactggagct tttgtatcga
     9001 tttctgacca aggtccaggc tgtgtgtctt tgattgacaa ggaaacacgt tcttgttcag
     9061 gattgataga caacactttg acatttacat catcattgac agccaataca tcacttggtt
     9121 ttgctacatg actatgtgaa atctctgaga catggactag accgtcaatt ccgcctaggt
     9181 cgacaaatgc gccaaaatca gtaagacgtg caacacgtcc actgagaaca tctccctcat
     9241 ggatagttga aagaatttct tttttctttt cttctctttc gttttccact accgctttat
     9301 gcgataagat caaacgattt tctgatggtt cgatttcgat gattttaaag ctcaatgttt
     9361 tgcctttata gtctgaaaag tctgcaacaa aatgatcttc caccattgat gcagggacaa
     9421 aaccacgtac accgacgtca acgactaaac cgcctttgac aacatttgta acaggtgctt
     9481 cgatgacctt gccctcttgg aaatctcttt cgatatcttc ccaaactttt ttagcatcta
     9541 aacggcgttt tgaaagtagg tagctgccgt tttctttatc tttgccgatc gtactgataa
     9601 cgactaaatc taatacatca ccgactttta ctacttcgct gatatcttct accggcaaag
     9661 ttgataattc ttttgccgga acgactcctt caactccagc cccttcgatt ccgacaatca
     9721 cttgtttgtc ttcaatgact aaaacttcgc ccttaacaac atcgccaact tttacttctt
     9781 gtacgctttt cattgcgtct tccattgact ggttgttctc ttgattttga tcaaattctg
     9841 tcataacact tgtcctccta accaacattc tgccgtaagt acggtactgt ttttagttta
     9901 attgattatt caaaaaaaat aaagcgattt cgttcgtttt ccttgaaaaa acaattgttt
     9961 ttcatttttt ttcttaattt aaaaaagaag ataacctttt ttcaaaacaa cagcttttat
    10021 tgctgcaacg acttctggaa tcgatttgcc agtcgtatcg atacgaacag catcctctgc
    10081 ttgcttcagc ggagagacgt cccttgttga atcataatgg tctctttcct gtatctcttt
    10141 cgtcagtgtt tcaagatcag tcggaattcc tttttcttgg ttctctttga aacgtctttc
    10201 tgcacgctca cttacgcttg ctactaaaaa gattttcact tctgcattcg gtaatacagc
    10261 tgtcccgatg tctcttccgt ccatgacaac gccaccgcgt tcccctattt ttcgttggac
    10321 agcaactaat tcttctctga cttttccatg tgcagagact tcagaaacag cttttgtgac
    10381 atctggttga cggatttcta aagtcacatc ttttgatgct atgaatacat gctgtccatc
    10441 ttcttgttgc gaaaaagaga taggatactt tttgatcagc gaaactagcc cttcttcatc
    10501 tgaaaaagat acattgttcg taatagctaa ataagttact gcgcgataca tggcaccggt
    10561 atctgtataa atataattca attcttttgc taatatcttg gcaacggtac ttttcccaga
    10621 tgaagcaggc ccgtcgattg caatactgat ttgtgtcatg atataactcc tttttctcag
    10681 aaaaaaagcc gcaaagcgcg gctattatta tttaattctt aattcttgac cagggtaaag
    10741 cataaagttg tttggatcta agccattcaa ttcaaacaat tgatcagtgg tgatccctgc
    10801 acgctcagcc acttgttttg gaccttcgcc tgcttgaaca gtcgttgttg aaccggctgc
    10861 agctgatgaa gaaggtgttg tttctgctgc attgctgctt gaatcccctg cttgataagt
    10921 tgcctcactt gaggaagtcg gtgttgtctc tgctgcaata ctgctttctg acgttacacc
    10981 tgtatcttga gacacagtag attcttccgc gattgtacta gattctactg ttgaagtctc
    11041 agttgactcc tcttttgttg tacttgtaga actttctact actgatgtcg aagtttcaga
    11101 agaagttgct gctgttgcat tgttgcttga tccatttctg atccagaaat aagcacctgc
    11161 tggaatagcg atacataaga ccagtaaaat caaaaatagc gaaagaaata atgtatttcc
    11221 tttcttttgt tgtctctgag aacttcttga tgaaacttca tcatctgtat cgtagattgg
    11281 ttgttcccat ggttcttgtg tttcgttatt ttctgtagat tgatgtctat cttttctgct
    11341 cactgtttta cctcctaaac tctatcgtgt gtattatacc ataggaaaaa gtgtcttgtc
    11401 ctaaaaaatc aatgttccat gtttttttta aataaattta gtaaaatcgt ttcccaatga
    11461 agttgttcag tcttagaaaa aagcttcctc tttggcagaa gttctgcttt tgctccatca
    11521 atatcacaac aattctgcgg cttttcggag agtttttcgc caaaataagc taagatgaat
    11581 tttctgcggc agttttcttc attaatatag cgaagcatct gctgcaggcg gaattctttt
    11641 tctttttcct gacgtttgag ccgttccacc caattttctg gtttttcaga ctgctggatc
    11701 agctccatcc attttttttg cagctcatca aagggagctt gctttgtggc atattccaaa
    11761 taaagctcaa aactttgtcg ctgttctcta gaaagttgat taaagaaata atggatacgt
    11821 tcatccccac tttgataaaa aagaattgct gcactttctt cctgatctct gcctgctcga
    11881 ccgatttctt gtacatagtt ttctagactg tctggcaaat cataatggac cacataacgg
    11941 atatcagatt tatcgatccc cataccaaat gcattcgtag caatgagaaa ctgcagctga
    12001 ttttttacaa actgctgttg aagttgtctt ctttgagctg catcaagacc accgtgatag
    12061 tatccaactg taaaacgttc cctgaataaa tgatacaatc gttcgacttc tttttttgtt
    12121 gcacaataaa tgattgccgc tccttgtgct ctttccatga agtgttcaag ttcttgttct
    12181 ttttgctgcg ttttccgaac aaaaagcgag atattttgcc gattgactga ttgtctgatc
    12241 tcttttggtg gtcgatcaaa tagtacatga cgtatatctc ttgctactaa atctgttgct
    12301 gtcgccgtta gtgccagtgt gaccgggttt cctaaaagct ttcggatttt tcctaactgt
    12361 tgatattctg gacggaaatc aactccccat tgagacacgc aatgagcctc atctactaca
    12421 tataacgcaa tattttgttt tttcagatgc tctaaaagag aaggattagt caacatttcg
    12481 ggactcaaaa acaaaaattt atattgcgat agatgacgga ggacgtatct tctttcagat
    12541 tgcgaaagta aactattgaa agcagctact cgtttctccc ctcgtttttg taattgcgtc
    12601 acttgatctt ccattagaga aagcagcggt gacacgatga tgaccagacc ttccattaaa
    12661 taccccgtca attgatagca aaggcttttt cccgttcctg taggcaaaat agcgagagta
    12721 tcttctccat caagcaacgc ctgaataatt tcttcttggc cgggacgaaa tgaatcaaat
    12781 ccaaaatatt tttttaattc atttattaac attctgtatc gcttcctttc tcaatagata
    12841 gatttgatac aaacgaaatt ctccataatc taattgtcca tctacattcc attcgctaaa
    12901 gcgccactct cgcacggaat ccttatttgg ttccaaacgt tcgattgttt ctgcagagag
    12961 aatccactcg tatggaaaat catcaaaaaa caaggcccac tcaataagat gatctgttac
    13021 tgttcctctt tttaaatgac ggattgccat gatctggtca attgtctgac cattcataaa
    13081 catctgcttg gtgatcatca tgctttgatt gaaattttgc tgtaataatg aatcgatcaa
    13141 agcgtgccaa agcgaatctg gccgtttttc gatttgtgct aacaaaaggt ggatacatct
    13201 gctttgttgc agataaacct ctgactcatc attgtttttt gccagctgat aaggaagcag
    13261 ccctgttcgc tcatttccag ataattggtt agctaaaaaa tctgctgctt cttctggtat
    13321 ctccgaaaac agctgcacca aactctcata cgcagtatca atcaaaattt cacgtggcaa
    13381 ttggctttca ctaagccatt tttttagttg gaaagtataa aaaggacttg tctctgctgg
    13441 aagatagtct tgaatacccg ttgccagatt agaaattact tgaacagcaa atttaatgag
    13501 ccgccaacta gtttcccctg ttctgccata gcggtcaaaa cgcaaaccag tatgttctat
    13561 ttgatgttcg gacaacatat cagcccctgc aggcgttagc tttgcttcat tttcatttat
    13621 ttcgatccaa ccttgctgcg caattttttg caaaatttga taaaacttat cctgcttcaa
    13681 ttctggaaaa ctgttatgga taaacaaaag ttcgtgggta aatccataaa taagtactga
    13741 agtcgttcgt tttcccacaa ggagttggta aagagaacta atccgcagct tgtgtcctga
    13801 tgaaaatagc gataaaataa aaacaatcat attattctcc ttaagttagg atggttagaa
    13861 atgtcactac tatgtaaact tgtacctgaa cgctgtattg catgcggtct ttgtcaaatc
    13921 gaagcgccag atattttcga ttatgatgaa gaaggaatcg ttttatttgc ccaagaacca
    13981 gaggctcatc aacagttcgt gatgccggaa gaagaaacat atgtaaaaaa agcgtatcaa
    14041 aaatgtccag ttcgagcgat tttattagaa aataaaatat aaatataaac gaaactttaa
    14101 aaacgtacac taagttctct aaacttgaaa ataagccggg atctgtttct cttgttccgt
    14161 gaaatcccga cttattttga aagattcatt tgctatctat agttaattga ctagtttttt
    14221 cacggaccct ataggaccca tttatattat ttcaactttt tcagccagtt ttccaatgta
    14281 ttgacgatca cagtttgctg gtctgcgtta gaaatttctg cctctccatc tcctttttgc
    14341 tcaccatagc tgccaaaacc tccatgattt ccgccattga ttgatacata ctctgttctt
    14401 tccggtaaat actgcttagc ttcttgataa gcttcctgat tcaatacttt atcttttgtc
    14461 gccgtgatcg ataaaacagg aacagctgat tctgtcaatg ttcctttttt gtcgggataa
    14521 cttgccatga agaatacccc ttttaaatgt ttgtctgaat gattcttatt gacataacga
    14581 cttgccatta cgccacctaa tgaatggcca ccgatcacat aatcggaatt tggaaaatct
    14641 tctttgacct tatccgcttt gtttccgccc atcactgcta gatcaagtgg cattttgaca
    14701 atagcgactg aaaagccgtt ttttgctaaa tcttgcgccc aaatactata actttctggc
    14761 tctacgagag caccaggata aaaaataagt aatggagact ttccatgatt ctttttctct
    14821 ccttcaaaaa ataagtaatc ttctttgttt tttccttctt ttaccaacac ttgcgcttct
    14881 gacgatggtt cgtacgtatt ttgctgtata tagtaataac cggctccacc gagtaatcct
    14941 aaaatgatga ctaagccaat tagtattcgt ctaaatattt tcactttctc ccaccttttc
    15001 tatctgagtg accccttttt cttaatcaag tatgattata ccttaaaaaa ataagagaga
    15061 aatgcaaact ttccagcatc aaataaaaaa agacggaata tccatttgaa tatccgccct
    15121 ttttgtatgt ttatttttat ttagcacata aacgatacac agcttctgca taaatttcca
    15181 ttgctttata catactactt aattcccatc gttcattcgc ctggtgcata aagtcaggag
    15241 tatcagggaa catcgcaccg aatgccacac actggttcat cgtacgagca aaagtagcgc
    15301 cgcctgatac aaacggttca gtcatgtctc cagtaatatc gcgatatgtc cccaatagat
    15361 ttttcactaa ctcactgtct agtggcacgt ataatgaatc aaggaagtca aattcttcat
    15421 aagtcaaccc atatttttct gctgttttag ttaatttagc tactaaatca tctttcttga
    15481 atgtaacagg gattctcata tctacaccga tttttgtttg ttctggtgtg atttccaaac
    15541 ttgcaaagtt catcgttagc tcgccggatt gttcatcttc tgttttgccc acgacagctt
    15601 caccagttgc attttcttga actaattttc ctaagaaatt gattgggcca aaatcaaata
    15661 cttctgaaag agcaattgcc agacgagaaa tagcattcac tccttgtgct gcatctttcg
    15721 cgtggatact tttaccaaga acgacgatgc cttcaccttg ttcttcgaaa tcgaattcgt
    15781 gttttttcaa tgcttctttt acttcagaaa gcttttctcc agagtaagga gctgcatcag
    15841 gaacaacatt caacgctcct ccagctttta cagaaaactc gttcgttcct ggtcctgtca
    15901 agtaagcttg caataagcct ttttctgcat aaattaaagg aaattcagca tctggcgcaa
    15961 atccttgcgt aattccttct tctttttcgt tatatttttc taaacaacgc cataaatttt
    16021 cttcatctgt accaaagata aaacgaatac gtgtcttgaa ttccacgcca gcatcaatca
    16081 aagctttgac ggcatacatt gccgcaatag aaggaccttt gtcatcttgg gatccgcgac
    16141 caactaacca gccgtctttt atagttggat cgaatggttt tgtttcccaa ttattttcgt
    16201 cccctgctgg aacaacgtcc atatgacaca agataccgaa taattcatct ccagagccaa
    16261 tctctgaata accgtaatag ccttctggat ctttaaatgt tctaaatcct agcttttcac
    16321 tgatttccaa tactttatcc aaagcttcaa tcactttttt accgaaggga tgtccttgtc
    16381 ctgaatctgc ttcatctagt acggaaggaa ttcggatcaa ttcgctcaat gattctaatg
    16441 cttgttcatg atgttgttct gtcacaaatt ttttcatgat attatcctcc taatatgtta
    16501 aaaagggtca tgtcaaattc ctccgacaca acctcttctt ctcaaataat gacgttttaa
    16561 gcctgctcca tcagcaagca gaatgttctt aacaaatgat gaaagaaaaa acactcactg
    16621 aatggagcta ttgacttgtc cacgtgctta tcgatagccg tttatcggtt tcttcattct
    16681 tatgatagac cttgtacctg tatttgtcta ggtttagaac attgctgcta cacctaggat
    16741 cacacagatg gctacaaata atatcgcaat taatttaccg gtaaatttcc accacactga
    16801 aacatcgaaa cgagcaattg ctacagcacc cattacgata gcagatgttg gtgtaattaa
    16861 gtttactaaa ccagatgcag attgataagc agtgattact aaatccttac cgacgccggc
    16921 aaattcaccc ataggtccca tgattcccat tgttgcagca gctaagccgg aagttgaagg
    16981 aatcaagaat gacataggga tatagaaaat aaatgtaata atgatgaata cacttgaaga
    17041 taatgaactt aatccttgtt cgccccaatg taagatggtg tctgtgatta atccattgtt
    17101 cataaccact tggataccac gagcaacagc tactacgatt gctacaccga tcaaatcaga
    17161 agcaccagct aaaaattcag agacaaattc agactctttc atgccataag tgattgctac
    17221 tacgatggac attactaaga ataacatagt aatttctggg aagtaccagt cacctaatgg
    17281 aagcatgctt ttacctagaa ccgtacctag taccggaagg tttgttaacc agtttgtcaa
    17341 tgattcaaaa atcgtgaaat tcgcattcaa ggttgtccaa ggaatcaaac tgatgatcat
    17401 gataccaaaa gtaataaaga acaatacatt aacatgtttt tgttttttcg tgattacttc
    17461 ttgacgaccg attgcttcaa tatcgaaatg cttcatatct tctttacgtt ggttgtatac
    17521 taatgatttt gttggatctt tttcgatctt tgatgcataa cgatacacat atacaataga
    17581 aatagctaac aaaataacga acatcaagaa tcgaagacca ataccttcac ccatcgaaat
    17641 cccaacagct tgtgaagcta cgcctgttgc aaaaggattt accgttgaag ctagacagcc
    17701 tacttgagac cctactaata cgattgctac agctactact gtatcaaagc ctacagccat
    17761 catgactggg atcagtaaag catagaatgg aatcgtttct tctgccattc cgtaagtaga
    17821 gccgcctaat gcaaataaca gcatcaatac tggaataagc attttttctt ttcctttgaa
    17881 tttcttcaca actgaagcaa tcccggcatc tagtgcccct gttttgttga taatacctaa
    17941 aaatccacca acaaccaaaa tgaagaaaga gattgggatt gcagcaggtg tttctttagt
    18001 tagctccccc gctggtacac ctagcatccc attgattggc gccatgaaaa tatcccacag
    18061 cccttgagga ttttgttgta ctcgttcata tgatccagcg acgatgttgc ctgcatcgtc
    18121 tacactatag tgcccagctg gtataaacca agtaagtaca gcgattactg caataataat
    18181 aaataagacc gtatacgctg aaggcatttg aaattttttc ttttttgttt gttccatctt
    18241 cttcttcctt tcttttccgt tagagacaac aattttggct ctaatgttat tttatcaaaa
    18301 agagtatcag aacaaaaata ataacacgca ttttgtaaaa cttttgttca aaaagttcgt
    18361 ttttccctat tccctcctct tttttcagtt ttttaaagct ttcttctttt tcattgtagc
    18421 gtttcctttt ttttataaac aaacattttc gtcttttaaa aggagaagaa tttcacattt
    18481 gcaaagaatt gattgatttt tatgcataaa tcaaaaaaat actcaaaaca aacaaataaa
    18541 acgtttacat tattgcatgt cttgtatttt tttccaaaaa aaaagaccaa gaaatttctt
    18601 gatctttttg atatttattt gactgctgta tgacgttgtt cgaactgatg ttgttttcga
    18661 cttaaccatg gaagaagttg cgcatgcagc actaaaaaga cagcactcac gatgaatcct
    18721 ttgatcaagt tgaaaggaac gatcccaatc agtacatagt ttgctaaaga catgcccagc
    18781 atctgtgttg cgttcagccc taaaaacatt aagtaaagag gcgtaataac gaaataattt
    18841 gcgatactca taaaaatagt tagagcgatg gttcctgtaa tgacacctaa aaatttgttt
    18901 cttcctttat gctgtttctt tacactaaag aaataataaa tcgggaatgt aaagatcgtt
    18961 gttgataaga aactagcagt gtctcctacc aaattatccg gagaaaaacc tgtagtcagc
    19021 agatgtaata ctgaacgcaa gaacgctgtt gcaactccgg ccgctggacc gaataagaac
    19081 atgctgatca ataccgagat atcactaaaa tcgatcttca aaaaactaaa agccggcatg
    19141 atcggaaaag caaaatactg taaaactaat gcgatcgcag caaacatcgc tactgcaacc
    19201 attttctgta cacgggtgtt cttcatttga aatcctcctg ttaggactcc tcttcagcga
    19261 actttttgac gtttctctcc aaatgaaaaa actctatttt ccagggaaaa tagagttaga
    19321 gttatttgat caagcgtcgt caaataagct ctatcttctt tatccagact atactgtcgg
    19381 tatcggaatt tcaccgattc agctattttt ttgcaaaata ggtcgtggac tataaccacc
    19441 ggtcgggaat ttcaccctgc ccctgaagac gaaccttata taatttttgt ttacatattc
    19501 attatacata tctattcaaa aaacgcaaca tctaacaatt ataaaaagta agtgttcatt
    19561 ttgagtggtt cagcaaactg ctgacttctt ttttgttcag ttcacggtaa tccccaggac
    19621 gtaggccttg taaagtcaga tctccgtatt tttcacgttt tagtttttgc acaggaagac
    19681 caactgcttg aagcatattt ttcacttgat gattacgtcc ttcgtggatc gtcaattcca
    19741 cgatactgtg gtttgtctta ggatccactg ataaaatttg gaatttagct ggagatgttt
    19801 ttcgtccttc gatctttatt ccttttgtca aaggtgcaag catcgatttt gtagcgatcc
    19861 cttttacctt tgcaacgtaa actttatcca cttcatgttt tggatgtgtc aggcgctgag
    19921 caaaatctcc atcatttgtc agtagcaaga tccccgaagt atcgtaatcc agtcgaccta
    19981 caggatagat acgttctttt accatcggca aaagatccgt aacaactttt cttcctttgt
    20041 catctgcaac agcagaaata actcctttag gtttgtacaa taaatagtaa ccgtattctt
    20101 cttgataaat tgggacttca tccacttcca cagtgtcatg tttcccaact tttatcccta
    20161 attcccttac aacttgtccg tttactttta cacgtcctgc aaggatgaat tcttctgctt
    20221 ttcgacgaga agtgatgcca gcgtgggcaa ttactttctg caatctctcc attacaatta
    20281 ttctccttta ttcattccat caaagaatag atctggtgac acgtcttctt caagtgaaat
    20341 ttccatttct tgtgtatcag gcagttcttc taatgatttc aaaccaaaat aatctaagaa
    20401 gtagccagtt gttccatata atatcggccg acctggtcct tctactctac ctttttcttc
    20461 aatcagttta tgggcagcta gacgctgtac cgctcctgaa acctgaactc ctcgaatctg
    20521 ctcgatttca gctcttgtga ttggctgttt ataagcgatg attgctaagg tttcgacggc
    20581 ggcttgagat aatgtgtttg atgtcccttg cgcatatcgc ttaagtaagg gagccagctc
    20641 ttttttagtc gttaatacat aatggctttc cgtttcaaaa atatgcaaag ctgagtgcga
    20701 gttttgagtg taagattctt ttaattcttt cactaaactt gtgacttctg cttcgctgag
    20761 atttaacagt ccgcttaatt cttcattact gatcccttcc tcgccaacaa taaaaagaag
    20821 ggcttcaagt tcacttaatt tcgtcatgtt cggtatctcc tactgctgga taaagcataa
    20881 ttgattcata gttttctttt tgttgcgcaa tgatctgccc ttttttcatc aattctagca
    20941 aagccataaa agtagtgaca acttcacttt tagaaggtga ctcaaaaaaa gaatcgaaag
    21001 tacagccatg cgcatttctt tttatacgct cttcgataaa taccattcgt tcttcgatcg
    21061 ttgtgtcatc gggcgtgatc gttttttcca ctttttgaga aaatttctta cgttctaaga
    21121 ttttctgcat ggcacggatc aaatcgattt ttttgacttg tcctcgcttc aacggctttt
    21181 cagcatccgc taacccatcg atgttcatag gttcttttgt gtaataaaga cttcgttcct
    21241 ctgctttttc tgtcagtctt tcagctgcat atttatattt acggtattct aaaagctgag
    21301 tgaccaattc ttctcgagga tcttcttccc ataattcttc atcgtctgtg ccgaattctt
    21361 ggacaggaag caatgtcttg cttttgattg ccatcaatgt agcagccatc actaagtatt
    21421 ctcctgcaac agctagctcg agtgttttca ttgtatggat ataattcata tattgctccg
    21481 tgatcgtagc gatcggtata tcatatatat cgatttccat ttgctgaatc agatgaagca
    21541 gcagatcgag aggaccctcg aatacatcta atttaatatt gatctccgtc aactcattca
    21601 ctctttcttc tttttctttg ctagccattt tccaatgtaa ggctctgtga tttcagttgc
    21661 acggatggag acatccgggt acttttcctg taattcatct aacatttgaa agcccatatt
    21721 ctcaccgcga taagacggat tcaaagaaat gtcatggatg atcagctgat cgttcgttgc
    21781 ccagtaaata ccaataatcc cttgaatgtt ggttgaacct tctggcgtaa agaaaaaaag
    21841 ttcataatcg tcttcagatt cgtacgtatc aatctccttc aacaaagaag ttttacctgt
    21901 cagtttctta tggaaactca atagtcccat agcaatttta cggttttctt tccggtaatg
    21961 tgttaacatc ttctcacctt cctatgacta agcccttgga aaatgctgct tataaacatc
    22021 cgtcattctt ttcttcgtga tatgtgtata aatctgagtc gtagagatat ccgcgtgacc
    22081 taacaattcc tgtactgtcc gcaaatcagc tccattttct agcagatgtg tggcaaagct
    22141 atgccgcagc gtatgtggtg ttacttcttt gtaaatccca gcctctcgta ctaactgctt
    22201 cagatttttc cagattcctt gccgagaaag acctttccca tgattattga caaatacatg
    22261 catttcagat gcgtccttta caagtaatgg acgtgcttcg tctaagtagc gttccagcca
    22321 ttggatcgca taatcaccta aaggaatgat ccgctcttta tctccttttc caactgtctg
    22381 taccagtcca agagaaagat gaagatctcc caatttcaat ccaatcaatt cactgacgcg
    22441 catcccagta gcatacatga cttccaagat tgcacgatct cttatcccta acgtttttgt
    22501 cgtatcaggt gcttcaatca gcttttctac ttctgtcaga gtcaatgtac ttggtaattt
    22561 ctgtgctttc ttcggcgtat cgatatgttg catcggatca tgatcagtga tacgctcttg
    22621 ccgtaaaaac tgatggaatc ttctgagact tgagatcatc cgagtaacag ttgctgtcgc
    22681 ttttttttct tcatgcaact tctccagaaa acggatgact aaaaagcgat cgacatcctg
    22741 ccatgaagca attttctgct cctgtagaaa atgcaagtac tgttccaaat cacgttcata
    22801 gctttgtcgt gtattggcgg acaatccccg ttcaatattc aagtagtgga gataatcgtt
    22861 gatttgctct ttcataataa tccccctcat gcctctctat catatcagat accgcttcat
    22921 tctgatagtc tttttccata atctgagcaa agaaaaagcc gtctagacac tcagacggac
    22981 tggtttcttt tttcggctct ataatttatg ggactgatga atcagaattg attcttcagc
    23041 cccattttca ttttaggtat taaaaa
//
LOCUS       DARBDV010000040        22833 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000040 DARBDV010000000
VERSION     DARBDV010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.040"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..142
                     /locus_tag="SCM56_001474"
     CDS             <1..142
                     /locus_tag="SCM56_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEG1110258.1"
                     /translation="TIVGSIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGK
                     FL"
     gene            215..331
                     /locus_tag="SCM56_001475"
     CDS             215..331
                     /locus_tag="SCM56_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEG1110259.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(386..2068)
                     /gene="recN"
                     /locus_tag="SCM56_001476"
     CDS             complement(386..2068)
                     /gene="recN"
                     /locus_tag="SCM56_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEG1110260.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(2086..2535)
                     /locus_tag="SCM56_001477"
     CDS             complement(2086..2535)
                     /locus_tag="SCM56_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEG1110261.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(2642..3460)
                     /locus_tag="SCM56_001478"
     CDS             complement(2642..3460)
                     /locus_tag="SCM56_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEG1110262.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(3469..4359)
                     /locus_tag="SCM56_001479"
     CDS             complement(3469..4359)
                     /locus_tag="SCM56_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEG1110263.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(4361..4585)
                     /locus_tag="SCM56_001480"
     CDS             complement(4361..4585)
                     /locus_tag="SCM56_001480"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEG1110264.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(4590..5927)
                     /locus_tag="SCM56_001481"
     CDS             complement(4590..5927)
                     /locus_tag="SCM56_001481"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEG1110265.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(5928..6782)
                     /locus_tag="SCM56_001482"
     CDS             complement(5928..6782)
                     /locus_tag="SCM56_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEG1110266.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(6879..7328)
                     /gene="nusB"
                     /locus_tag="SCM56_001483"
     CDS             complement(6879..7328)
                     /gene="nusB"
                     /locus_tag="SCM56_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEG1110267.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(7321..7746)
                     /locus_tag="SCM56_001484"
     CDS             complement(7321..7746)
                     /locus_tag="SCM56_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEG1110268.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(7992..9056)
                     /locus_tag="SCM56_001485"
     CDS             complement(7992..9056)
                     /locus_tag="SCM56_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEG1110269.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(9228..9473)
                     /locus_tag="SCM56_001486"
     CDS             complement(9228..9473)
                     /locus_tag="SCM56_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEG1110270.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(9612..9986)
                     /locus_tag="SCM56_001487"
     CDS             complement(9612..9986)
                     /locus_tag="SCM56_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEG1110271.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(10069..10983)
                     /locus_tag="SCM56_001488"
     CDS             complement(10069..10983)
                     /locus_tag="SCM56_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEG1110272.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(11004..11807)
                     /locus_tag="SCM56_001489"
     CDS             complement(11004..11807)
                     /locus_tag="SCM56_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEG1110273.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(11840..12829)
                     /gene="manX"
                     /locus_tag="SCM56_001490"
     CDS             complement(11840..12829)
                     /gene="manX"
                     /locus_tag="SCM56_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEG1110274.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            complement(12932..13423)
                     /locus_tag="SCM56_001491"
     CDS             complement(12932..13423)
                     /locus_tag="SCM56_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1110275.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            complement(13614..16421)
                     /locus_tag="SCM56_001492"
     CDS             complement(13614..16421)
                     /locus_tag="SCM56_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG1110276.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            complement(16855..17148)
                     /gene="rpmA"
                     /locus_tag="SCM56_001493"
     CDS             complement(16855..17148)
                     /gene="rpmA"
                     /locus_tag="SCM56_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEG1110277.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(17161..17505)
                     /locus_tag="SCM56_001494"
     CDS             complement(17161..17505)
                     /locus_tag="SCM56_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEG1110278.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(17532..17840)
                     /gene="rplU"
                     /locus_tag="SCM56_001495"
     CDS             complement(17532..17840)
                     /gene="rplU"
                     /locus_tag="SCM56_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEG1110279.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     misc_feature    complement(17860..17936)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            18122..18763
                     /locus_tag="SCM56_001496"
     CDS             18122..18763
                     /locus_tag="SCM56_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEG1110280.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(18825..20456)
                     /locus_tag="SCM56_001497"
     CDS             complement(18825..20456)
                     /locus_tag="SCM56_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEG1110281.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(20579..20938)
                     /locus_tag="SCM56_001498"
     CDS             complement(20579..20938)
                     /locus_tag="SCM56_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HEG1110282.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            complement(21097..21170)
                     /locus_tag="SCM56_001499"
     tRNA            complement(21097..21170)
                     /locus_tag="SCM56_001499"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21134..21136),aa:Arg,seq:tct)
     gene            complement(21479..22162)
                     /locus_tag="SCM56_001500"
     CDS             complement(21479..22162)
                     /locus_tag="SCM56_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEG1110283.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            complement(22290..22808)
                     /locus_tag="SCM56_001501"
     CDS             complement(22290..22808)
                     /locus_tag="SCM56_001501"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEG1110284.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
BASE COUNT         6565 a         5034 c         3807 g         7427 t
ORIGIN      
        1 gacaatcgtc ggcagtatct atggaatgaa cattaagtta cctattgctg aaagggatga
       61 tgcctttata tggctagttc tattaacatt atttatttgt atcgtaacta cctattattt
      121 aaaaaaaggg aagtttttat aaaaaagccc tcttgtggaa atggaaaaaa gattgtacac
      181 ttgaatcaac taattaatga cgaggttgaa aacaatgcgt gaaaaaaaac aaacaagtac
      241 ttttgctaaa gtaacaaaat ttgtcatttg gacaatgctg attttaacga ttggatctgt
      301 tgtattgact gctgtaatga gtgtcatgta aaaataaaat atagctatca gctgctgaaa
      361 aggacctgaa ccactgcgag gtggctcagg tcctttttgc gagtttcaat agttcttttg
      421 catgttcgag tgtgagtttt gtaatctcac ttccagccag catacgtgca atttcttctt
      481 ctctttcttt tgttttcaac tctgaaacag aagtttgcgt acgtccatca atcactgctt
      541 tcacaatata atattgatag tcggcgactg cagcaacttg aggcagatga gtgatgcaca
      601 aaacttgaga gtatttcgat atttgagaaa ttttatctgc gatggcctgt gcaactcttc
      661 cgctcacacc tgtatccact tcatcaaaaa taatacttgt cacaccttgc tcagaagaaa
      721 aaatagtttt caaagcaagc aacatccgag acagttcgcc accagaagcg actttcacca
      781 aaggcttcag tggctctcct ggattggtcg taatgtaaaa ttcgatttgg tcaaatccat
      841 catgattcaa ttgtctattt tcttctttta agaaacgtac ctcaaactca gtgttttcca
      901 tatacagact tttcaattca tgcagaatgg atttttccaa ttcttttgcg atttttttac
      961 gcgcttcatg aagatctgcg gcttgttgct gaagtagaag ctctttttgc tctagttcct
     1021 ctttcatttt gtccaattga ccttctgaaa aatcagaaga agctagctct tcggtgattt
     1081 cgtcatagta agccaatatt gactcaatgg attcgccgta tttacgtttc aactgtcgga
     1141 ttaattctaa acgttgcgta acttcttcta aacgactctc atctaactcc aataaatcaa
     1201 tctgacgact catatcgccg attgcatctt gcagcaaata gtaagcactt tggatatttt
     1261 cataaattgc ttcatactcc acatctagat gggcgatccc ttgcagttct gaaacggcca
     1321 agccaacacc atctacactg ttttgttcat tattcgtcag tgctccgtaa cctgcagcta
     1381 atccatcagc aattttttga taattgctaa gtttttctct ttcttctttt aatttctctt
     1441 cttctccgac ttgcaaatca gcagcagcta tttcttcttg ctggaaatgg agcatatcta
     1501 ttctctgaac ataggatttt tcattttgct gcacttttcg tacccttttt tccagctcac
     1561 gataagagat atattcttgc tgataactct ttttcttctg ttggaaagct acatctccaa
     1621 aacgatccaa taaagcaaga tgtgattcag gctggagcag ttcctgatgt tcgttttgtc
     1681 cttggatatc tactaaataa ctgccgattc ttctcagatt agctaatgtg ataatatgac
     1741 cattcactcg acaaacattt tttccagtta aggacatatc ccgtcgaaca atcagattgt
     1801 cctcatctgt ttcgatccct aattcaacca tcagttcaga gaatccttct tgttttggta
     1861 gttcaaacaa cccttcaagc acacattttt ctgctccttg acgtatataa tcgcttgaac
     1921 ctcttcctcc tgctaacaga cctaaagcat cgataataat ggatttaccc gcaccagtct
     1981 ctccggtcag cgcggtcatt ccttcgtgaa aagaaagccg caattccgga ataatcgcaa
     2041 agttagtaat actgatttct tgtagcatca cattcgctcc tcaacttaaa ggtaatgcag
     2101 gaagtctttc ttcaataaaa ctgcctggtc ttcagttcgt gtaatcatca aaacattatc
     2161 gtcatcattg ataatcgaaa ataattcttt cttgtaacgt ttatcaatca aatttgctgc
     2221 tgccgaagca tttcccggca atgttttcaa aataacaaat ttttccattt ggtcaactgc
     2281 cacgaaagcg tcttttaata gtttttccaa tttagtattt acatcttcgt ttgtttcagc
     2341 tggtatgcta taacggtagc cgccatctga agccggtacc ttgatcagtt tcagttcctt
     2401 tatatctctg gaaatcgtag cttgagtaac agatattcct ttgcttttca ggatttcaac
     2461 aaattcttcc tgctttcgga tatcttgctc actcaacaat cgagtaatca aacgatgccg
     2521 atcttttttt ctcattacgt cttcgctcct tagcatattc ttcttaatca atgatagctt
     2581 atttccttgg tataataaag aagaatccgc atacttctca cttgattttc acagttacaa
     2641 tttatttatg gtcaaactgt tcgtgtgcat ttgaaaccac ttctgccata ttaatagctg
     2701 aattgattgt tccgctttcg ggtactttcc tcaagtgagc caagaactcg atatttcctt
     2761 ctccccccgt gatcggagaa taacttaatt ccagcacatc atatccacct tgtgccgcaa
     2821 atgacaaaat gtcttctaaa actttttgat gggtctgagg gtcacgaaca attccgtttt
     2881 tccctacagc ttctctgcct gcttcgaatt ggggtttgat taaagcaatc acttctccct
     2941 cttcttttaa aatagcatgt aatggaggca gcatcagttt tagtgaaata aaagaaacat
     3001 cgataacagc aacttctgga attcctaagg taaagtcttc gggttttgca taacgaaaat
     3061 tcactctttc cattacgacg actctaggat cttgtcttat tttccaagct agctgattgt
     3121 agccaacgtc taaagcataa ctcatttttg cgccattttg caaagctgca tcagtaaatc
     3181 cgccagtgga tgcaccaata tctaacaatg tttttccttc aacagataga tcaaatgctt
     3241 tgagtgcttt ttctaatttt aatccccctc gagaaacata aggaagtttt ttacctttga
     3301 ctcttaattc actactcact ggaatttttt ctcccggttt atcaaaccgc tcgttttttt
     3361 cgttgtaaat caaaccagcc atcactgaac gtttggcttg ttctcttgtt tcaaataatc
     3421 cttgtttgac cgctaataca tctacgcgtt ctttttccat tctttccctc atttctccac
     3481 atcaaattgc ttgatcatct ggcgcatcag cccgccatca aaagaaaaaa cgtcctcttc
     3541 taatttgtct atgatcttgt tggcggataa cagctcaatc tccaacgcct cacgtgtctt
     3601 ttctaggcct aataaacgcg ggtaagtatt tttttccata cgttcatctt taccagctgt
     3661 tttccctaat gttttcgttg taccaatcac atcgagtaga tcatctctta tctgaaaagc
     3721 taaacctaaa tgttcggcta atcgttgaag tagcaataag atttcttcag gttgcttcgc
     3781 taatatgcca ccagccagca aggcataacg aatcaaatgc cctgtctttc tttcatgaat
     3841 aaatgctaat tcttctaaag aaagcttttt cgattcgccc tcgatatctg cagcttgccc
     3901 agcaaccata ccttgtgatc ccgcacaaac agctaattgc tgaagcagca gaagcttagg
     3961 agaattacct aagtgtgcca tactgattag ctggaatgct cctgtaagca aaccatctcc
     4021 ggccaatata gctaatgctt cgccgaatac tttatgattg gtaggttttc ctctacgaag
     4081 atcatcattg tccatcgcag gcaaatcatc gtggatcaag gaatacgtat gcaccatttc
     4141 taaagcagct gctacttgat agacgggaac atcaataggc tcatcaaagg aagcaacggc
     4201 cgttaaaagt aataagggac gtattctttt cccgcctgct ttcactgaat aactcatcgc
     4261 atcatagagt cctctatccg ttgtatgctc atctaaaaaa gacaagattt ctttttctac
     4321 agcaggtaga tgaatagctg aaaagtcagt taatctcatc tcagctttcc tcgtttccgt
     4381 caaaatcaac ttcttcattt gattctgtca tcattttcgt caaggtctct tctgctttag
     4441 ctaatgtatc ttggcaatgt ttgcttaatt ccattcctcg tttaaacgaa tctagagctg
     4501 attctaatgg aacgtctcct tgttctaact gcatgacaat tttttctaat tcttgcagtg
     4561 actcttcaaa agttggattt tccatatttt tattctcctt tgtctatctc ttgtacttgt
     4621 cctttaacga ttccatcttg ataatggatc gtcagcaaat ccccttgatg aatctcggtg
     4681 acacttttta ctacttgatc atttaacgtc gtatagctgt agcctcttcc catgattttc
     4741 aatggactca acaaatcaag agattgaaca gcttgagcaa aacgctgctg ctgttgtttc
     4801 atatattgtt ggattttctc ttctaagcgt ttttctagat aagcagtttc ttggcgagct
     4861 gctttaactc gataaagcgg agccacttgt tccaattggt ggcttaatgc gatcgtttgt
     4921 ttttctttcg catgataaat ttgctgtgta gcttgatata atcgctgttt caactggtct
     4981 aatttgatgg tctgaccttc atacaatctt tcaggctgcc gaaatacata ggaacgtcga
     5041 gcacgctcga accgttcttg tttgcgctgg atctggcgta caaacgcctg ctgcaatcta
     5101 gcttgccgtt cttgaatgcg catcaactct tctgacaaaa caggaacagc taattctgca
     5161 gctgctgttg gagtggcagc ccgaacatct gctactaaat cagcgatcgt ggtatctgtt
     5221 tcatgtccta cagatgaaat caccggcgta ttagcttgat aaatcgcccg agcaacccgc
     5281 tcttcattaa acggccataa atcctcaatc gaaccacccc cacgaccgat gatcattgta
     5341 tcgaaattgc ccatctcttc tactctctgg atattcctga caacattgtc cgctgcttgg
     5401 tttccttgta caactgttgg gaacaaaacc aattggacaa ttggatatct tcttttcacc
     5461 gtcgtgatga tatcgcggat aaccgctccg cttggactgg taaggaccgc gattcgcttt
     5521 gggaaacgtg gcagcatttt tttaggccct tgaaaaaggc cttccttcat cagcttttct
     5581 tttcgttctt ccaattcttg atataaagca cctattccat caggctccat gtgctctaca
     5641 tagatttgat aagaacctcc cgcttcatat aatgaaatac gcccaacgac aaggactttc
     5701 attccttctt ttggctggaa tcgtaatttt tggaatgctc ctttgaacat aatagcagat
     5761 atttttgcat ggtcatcttt taagctgaaa tattgatggg cattggcacg taaacggaaa
     5821 ttcgatattt cgcctgttaa atatacgcgt tctaaataag gatcggcatc aaattttctt
     5881 ttcaaatact tcgttaatgt agtgacagtt aaatattctt gtgccatcta ttcacctgct
     5941 ccaaaagctt cggcagattt tacggtttga tacatcagca tcgtgatagt cattggccct
     6001 acaccctttg gcacaggagt gatccagcct gcaataggct ccacttcatc aaatttcaca
     6061 tcaccgatca gcttaccgtt agcatcacga ttcatcccta catctatcac tacagcacct
     6121 ggttttacga attcttttgt gacaaaatgt ccacgtccta ttgctacgac taaaatatct
     6181 gcttcttgtg ccagttcagc cagattttcg gttttagaat gagcaacagt aacagttgca
     6241 tctgccatca agagtagctg agccatcggt ttaccgacga tattgcttcg tccgataact
     6301 actgctcgtt tacccgccgg atcgatagaa taagcttcaa acattttcat gatgccgtat
     6361 ggagtacaag gaatcttgtc cggtttaccc gcaaggagtc tgcctaaatt catcggatga
     6421 aaaccgtcta cgtctttttc agggcggata gctaacagga tcttttcttc atctatatgc
     6481 tttggcaatg gtaattggac caatatacca tgaaaactag gatcttgatt gtatttttca
     6541 atttctgcaa gtagttcgtc ttcagaaatc gtctcaggat aacgctctac caatgaatgg
     6601 attccgattt cttttgctac acgttcttta tttcttacat agatttggct ggctggattt
     6661 tcccctacca ataaaacgac taatccggga tggataccat tatctttcaa tttttggatt
     6721 ttttcagcaa tctctgcctg cattttttca gctaattctt ttccgttaat caattctgcc
     6781 atctaaccac tcccagatat tttttgtgtt ttcattctac catagaattt tcccgattta
     6841 taacaggttt atgatgattc atagaaaaaa gctaggactt acgccccagc ttctaattct
     6901 ttcaatacat tggataaaac accgttgacg aatttacgtg aacggtcatc actaaatgtt
     6961 tttgccaact cgattgcttc atttaaagca acagccgctg gtacatctgt cacataagtc
     7021 atttcaaaaa tcgcgatacg caagatcacc aaatccattt ttgagatccg attgatgtgc
     7081 caattgtttt ttaaatgttt ttcaatcact ttatccaatt catctttttt tgcacagaca
     7141 ccgccaacta gcgtatcaag ataaacagga acaaactctg attcatcttc gttgatcatg
     7201 tcgcggtggt ctaattcgat tgcgttgaaa atcgcatctt cttttgttag gtctgcattg
     7261 aagtctaatg gaaataaagc ctgcaatgcc atttcccgaa tctcgtgtct tgataattct
     7321 ttattcattc tcttcctctt cgtcttcaaa aagttcttcg attgttggtt gttctaattt
     7381 ttcaggaaca acagctacta catggatatt gacttcagat aaatcgatat ccgtcatgaa
     7441 aaggacttgc tggctgaccc gttcctgcat ttccattgct actttaggaa ctgaaacacc
     7501 atattctaga taacagtaaa gatctacttt caagccatct tcctcgtttg ctaagtaaac
     7561 accttttccg tgagctgcac gtcctaataa ttcagtgaca ttgctggcaa aagttccgcg
     7621 cattccgtat acgccttcta ctttagaagc tgcaatcccg ataattactt cgatgacttc
     7681 aggagcgatc acgatctctc ctaaatcttg gtttgcgttt aaaatcaaat ttttttcatc
     7741 tgccatttat ttattcctcc ctttgcctaa gcacgagata catccgaatt ctctatgatg
     7801 cggcatagtg atcacgtggt aagaaaactg ccgccgatca gctgcaaact gctatgaatt
     7861 ggagaaaaca cccaacttta ttatctctta cagcttaaaa gttcttctat agtgtatcat
     7921 taaatcgagt gattttctat aacaaagtct gtgaacactg atttttacgc ttacttcttc
     7981 tattgatgaa actataagat gatcaattct tttggtgaac gagtcaacac acgatttcca
     8041 tcttctgtga tcaacaagtc atcctcaata cgtactccac cgattcctgg taagtagatc
     8101 cctggttcat cagtgatcac atttcctggt acaaattgct tttctgcacg agaagaaacg
     8161 tttggtcctt cgtggatctc taaaccgatc ccatgtcctg tagaatgtcc aaatgcttcc
     8221 ccgtaaccat gttttgaaat ataatctcta gcaactgcat ctaattggac accagtcatt
     8281 cctggttttg cagcatcaat aactgctaat tgagcatcta agactatttg ataaatttct
     8341 tttaattttt ccccaggatc accaatagcg aatgtacgtg tcatatcgga aacgtaccct
     8401 tcgtaatagc aaccaaagtc caatgtgatc aagtcacctt gttcgatgat ttttttgctt
     8461 gctacaccat gcggcatagc cgaacgcaat ccgctagcaa cgatcgtatc aaaagaaaca
     8521 cctgaagcgc caagagaacg catataaaag tctagttgat ttgcgacttc aatctcagtc
     8581 atgcccggtt ggatcatttt caggatgtga tcatatgcca tatccgcaat atgacatgct
     8641 ttttcgataa tagcgatttc ttcttcatct ttcacttctc tgagttcttc gatcatctgt
     8701 gaaacaggta ctaattccgc atcgatgatt tcttctaaaa ccgaatattc taaaaatgag
     8761 actgttgttt cctcaaaagc taatgagtgc agttcttctt tgcgtactaa gtttgctacc
     8821 tcatcaaaaa taggtccgac atttttaata atttcaaacc cttgagcctg cgctgccgcc
     8881 tgctcggtat aacgaaaatc agtaataaaa aatgcttttt ccaaagtaat aacagctaat
     8941 cctgtcgtac ctgtaaagtt cgttaagtaa cgaagattgt acggactggt aaccaaaaat
     9001 gaatccaaat tttcttcttg catttttttg cgcaattttt ctacacgtag catcataatg
     9061 aaactccctc tttctttgca cgcttttttc cattatagca aacttttctt cgtacacgac
     9121 agttaaataa aaatttccgg aaaacttctt tttattttaa ataaataaag ccaaaaagga
     9181 gtcacagtct cgctgcaact ccttttagtt tactattttg taaaaactta tgatcgtttt
     9241 ccaaagaaaa atgagacaac tgctaccaaa atcactgcgc ctaaaataga tggaataagt
     9301 gccatacctg cagcttttgg tccccattcg ccgaaaatcg cttgtccaat acttgaaccg
     9361 actagtccag caatgatatt cgttacacag cccatcgaat ttcctttact ggtgatcgca
     9421 cctgcaattg ctccgatcac ggctccaaca atcaatgtcc ataaccaact cataatataa
     9481 cctccttctt tgctaatcaa ttttagcata tgggatttaa aggggacaaa taattggttt
     9541 tgctatttca tattttcttc tgcaaagtaa aaaaataaag aaaacctcct cttagaagtt
     9601 ttctttattt tttacttgcg tttaaacata ctacgaatac cacgtttcac tacatcaaag
     9661 aaactcaatg attgtaccat tctatcttca tctacatatt ctctgatcgc acgaagcact
     9721 tttttaggtt ctttggaaga aaatgtgtat gtaccatttt ttttcgtttg aatggcatat
     9781 cgaggaatcc attttccctt tagcatgact gatgctagta cataatccac ttcttcccat
     9841 ggaatttgga tatatttgcg tctatctcgc gcatggtaaa actcaaaacc attatcacct
     9901 atcatgattt gcccgtaatc tgtcagaccc gtaaacgaag tagcatcaat taccaagtct
     9961 acttttgtat tgattgattg aaccattttc tccctccaga tatttgttta tgaacagtat
    10021 actatataaa aaaagctcgg gacaacgccc gagctatatt tttatggatt ataaaagtcc
    10081 gatgacgtgt ccaacaacac cgactacgaa taaaccaagg atgataacga ttggagaaac
    10141 ctttttctta agcaaccaca tacataacaa tgttaatcct aatgcagcta aaccaggaat
    10201 caattggtct aagttgtctt gtaatgttgt taccttttca ggagaaaggg ctaagccatt
    10261 attaacttgt tcaaatgcac tcttgattcc ttcaccacct gaaggcaatt tatcccattc
    10321 gatgtaagca cctttatcta attttacagt tgatacaact ggtaagaatt tgattgatac
    10381 ccatctttgt acaagggcag ctaatacgaa catccctaaa attgaagcac cttttgttac
    10441 gtcttgtaac aatccgccag aaaggtcatc agtaatttta gaaccagctt tgtagccaaa
    10501 ttcttgtgta taccacatga atgcccaacg aataatgttc caacctaaga agaagatgat
    10561 tggtccaagg atatttcctc ccatagctaa tgaagcacct agtgcaccta gcattggacg
    10621 tactgtaaac cagaacactg ggtcaccaac accagctaaa ggacccatca tacctacttt
    10681 aaccccttga atcgctacat catcaacagg cgcgccattc gcacgttctt cttccagagc
    10741 caatgtaacc cctaaaattg gagaagcaat gtatgggtgt gtattaaaga attccaagtg
    10801 acgttttaat gctgcagaac gttcttcttt tgttttgtat aattttttga ttgctggaat
    10861 catagaaaat gcccagccac cgttttgcat acgttcgtag ttccatgaac cttgaatgaa
    10921 ggtagagcgc cacgcaacag ctaaacgatc tttttttgat aatttgattt tttcttctgc
    10981 cattttattt tcctccttca agattaatag tcattcaaaa tgtcgcctaa agggtcacca
    11041 gagttgcttc cgcctccatt atttgatgaa ccacccattt tagaaaggtt caagtaaata
    11101 agagctaatg caacacctaa agcacctaga gcaatcaatg ttaattgaga aattgcagct
    11161 acaacgaaac caataacgaa gaatggccat acttcttttg tagccatcat gttgattacc
    11221 aaagcgtaac ctacagctac gaccattccc ccaccgatag ccataccatc tgttaaccat
    11281 gctggcattg attcaaggaa tgattgcaca gtttgtgctg ggataaatag caacgctgct
    11341 gctgggaccg cgatacgaac cccttgtaaa catacagcaa tgatgtgcca catttcaacc
    11401 ttacggatgt ttccttcttc agcagccgca tccatcaaat gaacgattgg aacagcgatt
    11461 gtacgtacga tcattgttaa gaacaaccct gcaacagcca aaggaacggc aatcgcgatc
    11521 gcagaaggaa cacctttaac accttgtcca cctaaaacta aaataattgc tgatgctact
    11581 gatgctaatg cagcatctgg tgctacagcg gcaccgatat ttgcccaacc taaagcgatc
    11641 atctgaagcg taccgccaag tacgatacca gcttcgaggt taccggtaac taaaccgatc
    11701 aatgtacatg ccactagtgg ttgatgaaat tgaaattcat caagaatacc ttccatacca
    11761 gctagaaagg caactaatac gactaaaatt attgaaataa tagacatgat aagcctccta
    11821 taaattttaa tattatttat tacgcttttg ctaattcagc ttttgctttt ttcaaaatat
    11881 catccatgtt atcgcgtgaa tcattaggta ctttacgtac atcgaatttc acaccttttt
    11941 gttcaagctt ctcaaacgct tcaacgtctt cttgtcccat agacaatact ttacttacaa
    12001 cgactttacc gaccgaatgc gccattgacc caacatttaa ttctttgatg tctacgccac
    12061 cttcaacagc tgtcaatacg tcttcaggat tttcaaataa aagcaatgct ttggtatttc
    12121 caaaacgagg gtcttttgct acttcgatca ttttgctaat aggaataaca tttgctttta
    12181 cacctggagg agcagcttgt tcgattagtc tcttacgcag atcatccttt gatactgcat
    12241 ctgaaacaac gataatacgg tttggttgca ctgcttttgt ccaagctgtc gcaacttgtc
    12301 catgtaacag acgtgagtca acacgtgcta atacatattt gatttttccg tctccgacca
    12361 cagttccttc tggaagtgct cctttaggtt gtgcgtcttc aactgctgct ttcggtgctt
    12421 ctgccggttc taattcttcc ggttttactt ttacaccgtc ttttgctgtt tcaatgatgt
    12481 gtgctgcaat ttcatgagca gattccattg aaaagcgtga tgcgtaggct tcaatcaaca
    12541 ttggtaaatt caaaccgctg acgattgccc atttatcttt gtgctcttcg aataatgtgt
    12601 ttgattgatt gaacggtgtt ccgccccata aatcaactaa gaataacact tcgtcttgat
    12661 tatcaaaaga agcaaccgct tcttccaatt tggctctcaa gtcatctgga ccttcacttg
    12721 gtttcaaaat cacggctttg acgttttctt gttcgccaaa gatcattgaa ccggattgta
    12781 agattccttc agcaaattgt ccatgacttg ctaggataat ccctaccata tttatacctc
    12841 ctatttactt ttgttgtaac aaattcaaaa atattaataa ttgctaacca ttacaacaga
    12901 attatttgac cttttccaac aaccattctt gctacacctt tttgattttc gctagtagtt
    12961 ccatcaaaag gattttcctg tcagcaggta cttttcgaat ctctaactcc acttcttgac
    13021 tgttcaagta acgaaaggaa tcaatatctt gttgatttac tgaaacaaaa ttggtaatca
    13081 ttgatttgcc ttcagtaaaa ctcataccgc cgatattcaa cgacttcaac tgtacaccgc
    13141 agcggacaac cgcttcaaca tcttggggat tggtgaataa aagcatcact tttgctcgtg
    13201 ataataatcc actatgatag atttgaatca ttttctgcac ggttattaca tttgctgtga
    13261 tccccggcgg tgcagcttct tttagtagaa actttctcaa tttatcttga gcgactacat
    13321 cgctgatcac aatgatacgt tcaacacctg tttgcttcga ccaaactgtt gcgacttgcc
    13381 cgtggattaa acgatcatct atacgtgcca atcgaatatc catctgcaaa actccttctt
    13441 cctcactact tcacagaaac ccctatcatt tctgtgtgaa caaccacctc ctagcacaca
    13501 ctactataag caaaaagcgt gccaactttt ttacgataca ctaattgaat ttaaaggatt
    13561 cttaaccaat acacaaaaac gatacactat aaaagtgtat cgtttttgtg tattcatggc
    13621 acagtgtatt cttttttatc taactgatta tggactagac gtagcagata atacatttct
    13681 tctatcggca cttttacttt tatctgatct tccaacagaa caactgaggc tgccaattgg
    13741 ctataaagag gatcattcgt tgtatttgtc aattcgtttt cttccacagt caatggttca
    13801 ttccgaataa tacgttcgat cattcctgct gtgtgcatca ctaggttgat tttgaatcca
    13861 tattcttcat cgccgattcc cgtcgtctga gagatttgtg tagaaaattg ccacattgga
    13921 tcgattaact tactaccatt aataaagatg aagttatcag agataaactc aacacacgta
    13981 tttttagctg actcttcatc tagggagatg aattcagttt cctccaattc tgattccatc
    14041 agcaactgat ccaaaatgac ttcgatattt tgatcaataa aacgttctaa aggaataaat
    14101 ggggcttgga tattcaaatc agtaatccca gtagtagcta tgatctgata ttctttttgg
    14161 ataactggca gttcttcttt cacttccaca atcgacaaag ctagaacttc taattctgtt
    14221 tcttgacgct tgtttaccgc tcgttcgatc aattctttga ttctttgtgc tgtaccttcg
    14281 ccagatgcgc agatagccaa aacagctttt ttcttatgaa taggttttgc ttcctgaaca
    14341 gattctacag aagatatttc tgcgtaacct cgaaaatttt tcaatgattc atgcaacata
    14401 tctagatctg tccctaatac tgagaccttt cgtacagttt ccaaaactag agaagttgtt
    14461 accatatcaa cagtacgtac tggaattcca gtgtctcttt gaatttgcga attaaacgac
    14521 gcaagggacc ccatatctac taagagtatg accccgcttc cttcatctac ttcttgtaca
    14581 ttgcgttcaa ttcggtccaa tgctgttttc ggatccatat ccaaaggcat gtctactgct
    14641 cgtacgttct ctgcatctaa caactgttgg acaacttgga ccatactgct tgctgtgctg
    14701 tttccgtgag ctgcaacgac tacaccgatt ctgccgcttg cttgatttgc ccgtaacgag
    14761 acgagtagaa cggtcaagta atagtcttca ctttctggaa tctccacttg aaaatacgtt
    14821 tcgatataac tacgcacttc tttcgcaaca gcatattcat ctggaaaatc aaaagccatc
    14881 tcacggatgt tatcattcgt atgccgttct tctcctaagt tgattttttt caaaaaagaa
    14941 ctgatatgca agctcatagc ataaataaag ttttgctgaa actcatatgg taaagatctt
    15001 ttgaccatta cataaatctg attggtcact tctatgattt tcggatctac aaattctgct
    15061 aatttgctgt ctgcattaaa agaaaaacca tggtctttat agaaagactt cagatggatg
    15121 ttgatatctg tagaaatgaa atgattgatt gcttcttgat ccaaaccatc cgacttcagt
    15181 aaagctgctt tatctccgat gatatcatat agattataag gtaactcata ggagtccgat
    15241 tggattttca tgatcgtatc atttggtgct acagttatct ttggctccaa tattttagaa
    15301 agctctgcca ttgcactgcg gttgcttgct aattgaatca atccgctacg aatcccttct
    15361 gtcaaatcat cgattgtgat cgagatttcg ggtgattgca tatgatttag aaatcctcgt
    15421 gcacatacta actggatatt tgacttcaat tgtcctatat ttccataggt cacactgccg
    15481 atcaatgctt ttgctacatc ttctgtcaaa gaaatctttc gctgcgtgcg gtttgcttcg
    15541 tgggcaatca tgatttttac taaatcgact ttctcatttg caggacgttt ttcaaaagcc
    15601 ggaagtttga tattgattgg gatacgccga acaaaagttt ctaatagaga agaacctggg
    15661 tcttctgtgg tcgcacctac gatccgtaca tttgcttcat gggatttcgt tgtttcccca
    15721 agacgactgt aagttccatg atccataaag taaaagatca tttcttgacc ttcaggcggc
    15781 aaccggtgaa tctcatccaa aaacagcata ccgccatccg cttgatcgat aatccccatt
    15841 ttttcttcat ctgcgcctgt aaaagcacct tttgcgtacc caaataaatg actcatcaaa
    15901 agctcagggt tattggcata atcagcacaa ttgaaaacaa tcagttccgt ttcctcatct
    15961 attacatgat gcgcttttgc aaaatgatac atcgcgtgtg caaaataagt ctttcctgat
    16021 ccggtaggtc ctgtaatcaa acaatttaat cctcttggcg gatacaaaat cgcagctttt
    16081 gcctgttcca ctgaattttt catgctgccg ttagctccaa tgatttttgc aaagatatcc
    16141 ttagtatcta tttggagtgt atttttttct tcagagaccg gcgtttcttt gtaactgact
    16201 acatgttttg taactggctt tacgtgcgta acgagtgttt gatatatgta acgtactggt
    16261 cgtccgtctg tctttatcag ttttccttcg cgcactagct gattcagatc tttactgcta
    16321 tttgttcgct gaatccctaa cacttcagca atttcctgtg ttgttactcc ttgatcatat
    16381 tctgcctttg ttaaatgagc ggttttttct ttgacataca tataaattcg gtcaatgcgt
    16441 tttgccaatg gtatcgctcc ttttctatag actattgtac ttaggcttca aaacataata
    16501 ctatgtattt atttctgttt cgtcaactat ttacctgatt ttgttttgtt gataacacgc
    16561 ggatagatag aaatgttcaa tttaaaagaa cgaaaaatcc cgatacgatt tttataatgg
    16621 aaacaagaaa aagaacactg tttaccatat aattatttca ctgatagcaa acaatatagc
    16681 ttccatcaat tgaaaacgat agttatcaaa acatcctgat tccaaattgg caaaaaaata
    16741 ttaagtgtaa aaagacaaag taaacttcgt agcctccact acaactttta tttttataaa
    16801 caaaaaaggc tgtgacaaaa gtcttgtcac aacctcttta atcataagat tcaattaagc
    16861 ttcttgagct actggataaa cagacacttg ttttttgtta cggcctttac gttcgaaacg
    16921 tactacgcca tcaacttttg caaataaagt gtcgtctcca ccgataccca cgtttacacc
    16981 tggatagatt tttgtaccgc gttgacggta aagaattgat ccacctgtaa ctgtttgtcc
    17041 atcagcacgt ttagcaccta gacgttttga ttctgagtca cggccgttag atgtagaacc
    17101 gccacctttt ttgtgggcaa ataattgtag attcatagat aacaacatgg tctgcacctc
    17161 ctatttatca ttaattgttt taatttggat atattctgag ttttcagctt ctatcgattg
    17221 taatcctaat aaaagatttt ctaatagaat ctcagcaata ttgttttgtt cttgtgtcat
    17281 tcctgaagtg acttcggtat aaagataacc gccttcttct tcattcattt caacaatcgg
    17341 ctcaaaaccg gctaatgagg caatcccgtt gacagtgctg atcgctaacg cagaaacacc
    17401 cgcacaaacg atatcacttc cataaggacc agcatcagca tgcccggtca aagtgaatga
    17461 aacgatctga cctgcgtcat ttcgcttaaa tgttcctttg atcatatata ggccttcttt
    17521 ctcaaacgct attaagcgtt gatcgcgtcg ataaccactt ttgtatatgg ttgacgatga
    17581 ccttgtttac ggtgtgaatg ttttttaggt ttgtatttga aagtaacgac tttcttttgt
    17641 ttgccgtgtt tttcaacagt accttcaaca gttgcacctg caacagttgg agcgcctact
    17701 tttgtagatt ctccgcctac taagataact tcgtcaaaaa caactttttc tcctgcttca
    17761 acgtcaagtt tttcaacata gattgcttgt cccacttcaa cttttacttg tttaccacca
    17821 gttttaataa ttgcgtacat gctataaagc acctccttct gatatactta gactcgccat
    17881 ccgaagtgac agcatatgct gtgcttatta cttgttctga gcggttgtag ctgtggagtc
    17941 cacaattaca acagtactag cttaccagaa cagcaaccgt tagtcaacta aatttaaaat
    18001 caaaaagtaa aacaaaaatt caagcaatac ttctgctttt ctatcctcac agttctacca
    18061 aataaaatag tcgcattgtg tttcttttat tcttataata aaagaaaaag gaggagcata
    18121 aatggaatac ccaaaagaac tgatacaaga agcttcttta agaatgaacg gccgtcctgt
    18181 agaaggattt atcgctgggc aaggacctct tcatcctaaa ctgatgctcg tgggagaagc
    18241 cccagggaaa acagagattg atacgcatgt tccttttagc ggtcaagctg gaaaagaatt
    18301 gatgcaggca ctttcttcaa ctggcttaac acgagaagag gtgtatatca ccagtgctgt
    18361 aaggagccgc ccgtatcgcg tgactcatcg gatcaataaa cggacacaac agtcagaaac
    18421 tgtttatcca aatcggacac ctactcgttc cgaagtattt gcgcatgcac caatattgga
    18481 ttacgaactg atgaaggtcc agccaaaatt aattgcgaca cttggaaaca ttggtcttca
    18541 acgtttactt ggcaaagaat atacggttac caaaaatcat ggtcaactct atacagggcc
    18601 tgtcttacaa ttaacagaac aaaaggatgc ctacgaatgg tcagaaaaaa actatcgaat
    18661 tcttccactt tttcatccag cagctatttt ctacaatcgc tcccttgtac ctgttatcca
    18721 tcaagactgg caagtactag cttcactttt acagcaatat taaaaagaac agacaagacc
    18781 ataaaatctt cagtttacag tcttgtctgt tctttttcat tgttttaatg gctaattgca
    18841 gtatatttcg catgtaaagc tgtcaacgca tgagcaactg tttgatgttg atgcgtgaat
    18901 gtatgttccc acaagtgaga aagttgtttt tctacttctg gctgcaagga agtatagata
    18961 atcgtttgtc ctctttgctc tgctttttct tgaaattcat caaaaactgc acgtgccgtc
    19021 aaatcaatac ttggtacgcc tcgcatggag aaaatgacac catcgtgatg atttagttct
    19081 tccaatttat ttttgagttt ttctgaagac ataaaaaaca gtggaccact aatatacact
    19141 actgtccagt tattcagctt ttccgtttct ggcagcccca ttctttgcca atcgatcgtt
    19201 tctaatgaaa tcgtgatagc agcgcttttg acaacaaaaa atacacagcc gccgatcacc
    19261 ccgatcacga tagcaatact taaatcaaag ataactgtac aaatcatcgt aaggataaac
    19321 aatagaactg ctgaccaata tttattagta aatagctcct tgatcgtttc ccattcattc
    19381 atacgccaag ccgtcacaat caaaacacct gcaagtgctg gcattggaat ctgtgacatc
    19441 actggcgcaa aaacaagcat ggagagcagt aagacaactg catgtatcat accagctaat
    19501 ctagtttgtg caccagattt gatggccacg cttgtacggg cgatggccgc agtagcagga
    19561 atcccgccaa agaaaggaag caacagattt ccaattcctt gcgccattaa ttcttgattg
    19621 ctatctaagg gcttatttgc cattcggcca gccgaagcac cacatagcaa gctttcaatc
    19681 attcctaata aagcaatgct aatagctgga actgccactt gttgcaaggc agaaaagcta
    19741 aaatctccca tattcaaacg attactactg atcaatgttt gtggaatctt acccacagtt
    19801 gctactggta aatggaacaa tagcataact gctgttgcca aaatgatagc cagcagcgaa
    19861 ctaggcattc gtttcgccca tttttttgga tagataaaca tccctaaaat gactagccct
    19921 cccatgatca aagaggctgt gtttatcgga aatcctatcg tttgataact acttagtttt
    19981 tcaataacgt ttgtaccttc cgagtgaaca ccaaataaat tatcgatctg acctaatgca
    20041 ataatgatag caatacctga agtaaacccc gtaattacgg gtgctgggat aaaagaagtc
    20101 agtgttccta atcggaaaag tcctgctgca agtaaaaata gacccgctaa aaaagtagca
    20161 agcaatacac cttgcattcc ctttgttgcc gcaatagaca tcaagatagc tgccatcgca
    20221 cctgtcggac ctgatatttg ataaaaaccg cctgataaac cgccaataac gataccggca
    20281 atgatagccg taatcaaacc tgcggccgca tcagctccgc ttgatacccc aaaagccaag
    20341 gctaaaggca atgctacagc agctactgtg atcccagcca aaagatcttt ctgaaatctt
    20401 gaaagatcat aacctgaaaa ttcttttttt agtgaggaaa cataattttt gaacacctat
    20461 ctaactcctt tttcatgaaa ttttctaaaa taccattcat tagaatatca tatttcatga
    20521 gataaagtaa tatttttcac aaagtttcct gttagataaa ttcttatttt ttctatattt
    20581 ataagaaaat agaaagcaat gccaacagag ctagcccacc ttgtttgagt aaaatccaac
    20641 gatcgctagt caaactgcca tataatgcaa caaaaaaaat agatattaat agcatacttg
    20701 tgatttcttt agatgcggaa gaaagataag ctccataaag tagtcccact cctaatagtc
    20761 cattgtacac tccttggttc ttaaacaatg tttgaacaga ttttctgctt aactcttctt
    20821 tactcatttt aaaaacccta cttgtcgtgt cagaatcagt tctcaccgtt tcgatataca
    20881 tgatataaaa aaattcaatt gcaactagag tcactaaaat tctagatagt atcgtcatat
    20941 cttcttcctc ttttctgatg agttttattt ttttgctcct tataattgta ctcgataagg
    21001 atgttttttt attttatttg cctttgaaaa caaacaatat tggcacaaaa aacaaagaaa
    21061 aaccgttaga aacctcagtt tctaacggtt ttttagcgtc acaggaggga ttcgaacccc
    21121 cgaccgtccg cttagaaggc ggatgctcta tccagctgag ctactatgac aagaatcagc
    21181 ggaacatcaa taattataga taattaaatt gcttctgtca atacgaaata gttatttaat
    21241 tagcaaataa tgaattattt tggcccaact actaaatcaa tcttttaaaa cgagacattc
    21301 tccttcagct gttctaaggt tatcaattga aaacaggagc tacattgact tcatgctact
    21361 cctgtttatt tagatttcct gtatccttta attgacttac aaatatagaa acttttacat
    21421 caagttcgaa ttgagccctc gattcaatcg attatctaca ggctgccata atcatgtatc
    21481 acaactcgat tgtttctgat tcctttgtca ttaagatcac accatcacct aatggcaaga
    21541 ttgtcgatgt caaatcggga tgatccatca caacacgaag aaattcattt aatttacgat
    21601 ggatagctcg tttgccacgg ggaatctctt catctgggag caagatcgtt cctccttgga
    21661 agatgtcatc taccatcaaa acgccacctt ttcttaacaa acgtaagcat tcaggtaaaa
    21721 attcaatata ttttgatttt gcactatcca taaaaataaa gtcatacgga ccagacaact
    21781 ccggcaagat atctgctgct tgaccttcaa gcaaagtcac tttatctgtc agtcctagac
    21841 gttcatagtt ttttttagct ttctcgatca tgacatcgaa acgatcaatt gtcgtaacat
    21901 gagcgttttc tccgattgtc gtggccatga cacttgatga aaaaccgata gctgctccga
    21961 tctccaagat tcgttcaggc tgaagctgtc caagtaaaaa tttcaaaaat acaactgttt
    22021 catggggaat aactggtact tctgctacat gagcttcttc ttccagtttg cctagctcac
    22081 ccggtaattt tttttgctgg gtacgcataa aatccaatag ttcttctttg actacaggac
    22141 gatgcatcat ttcattgcgc atactactcc tcctcttcac acattatacg aacaactgac
    22201 taaatctaca agctgcatga gtgttttctc gtgtttcatg ttcagaagag tcgttggatc
    22261 tctggaatac atttatgttt ttaacattct tacaaaaatt tctgtgttcg tctccgcttc
    22321 tttttcaact ctacaaaggt attccatgct gtgattcctg ttaggcaaaa agcaatcgta
    22381 aagaataacc cctgaatcca tgcaaatgcc gtttttgaaa ggactagtcc aatatatgga
    22441 atctgaaaga tgggactacc gataatattt ctctttttta ctactccagg ctcaatataa
    22501 gtctttgtat cagcttttgt ataaaaatct tcgtggttgt tttcaacggc tattacgcga
    22561 cgcgtcgaga ttttttttcc tgaattttca taatatgtaa taatatctcc gactaaaatc
    22621 tcatttgtat ttttcggtcg tacatatatc agatttcctt gtgcataata tggactcatt
    22681 tctttgtcct caataatata aggagtcaaa ccaaaaattc ggggaaccaa gacgacgaga
    22741 agaataagaa ttaaaaatat aatcaacgtt atagaactta taaaagttat ttttttgtaa
    22801 tcaggtatcg ttttcaactt ctttctttat tca
//
LOCUS       DARBDV010000041        22706 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000041 DARBDV010000000
VERSION     DARBDV010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.041"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            123..935
                     /locus_tag="SCM56_001502"
     CDS             123..935
                     /locus_tag="SCM56_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEG1110285.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            932..1732
                     /locus_tag="SCM56_001503"
     CDS             932..1732
                     /locus_tag="SCM56_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEG1110286.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1893..3161
                     /gene="rseP"
                     /locus_tag="SCM56_001504"
     CDS             1893..3161
                     /gene="rseP"
                     /locus_tag="SCM56_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEG1110287.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3229..4938
                     /locus_tag="SCM56_001505"
     CDS             3229..4938
                     /locus_tag="SCM56_001505"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEG1110288.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            5150..9508
                     /locus_tag="SCM56_001506"
     CDS             5150..9508
                     /locus_tag="SCM56_001506"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEG1110289.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9653..10126
                     /gene="rimP"
                     /locus_tag="SCM56_001507"
     CDS             9653..10126
                     /gene="rimP"
                     /locus_tag="SCM56_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEG1110290.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10149..11324
                     /gene="nusA"
                     /locus_tag="SCM56_001508"
     CDS             10149..11324
                     /gene="nusA"
                     /locus_tag="SCM56_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEG1110291.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11346..11639
                     /locus_tag="SCM56_001509"
     CDS             11346..11639
                     /locus_tag="SCM56_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEG1110292.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11636..11947
                     /locus_tag="SCM56_001510"
     CDS             11636..11947
                     /locus_tag="SCM56_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEG1110293.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11960..14266
                     /gene="infB"
                     /locus_tag="SCM56_001511"
     CDS             11960..14266
                     /gene="infB"
                     /locus_tag="SCM56_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEG1110294.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14293..14640
                     /gene="rbfA"
                     /locus_tag="SCM56_001512"
     CDS             14293..14640
                     /gene="rbfA"
                     /locus_tag="SCM56_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEG1110295.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14797..14970)
                     /locus_tag="SCM56_001513"
     CDS             complement(14797..14970)
                     /locus_tag="SCM56_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1110296.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            15418..17780
                     /locus_tag="SCM56_001514"
                     /pseudo
     CDS             15418..17780
                     /locus_tag="SCM56_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            18148..19070
                     /gene="truB"
                     /locus_tag="SCM56_001515"
                     /pseudo
     CDS             18148..19070
                     /gene="truB"
                     /locus_tag="SCM56_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
     gene            19074..20015
                     /gene="ribF"
                     /locus_tag="SCM56_001516"
     CDS             19074..20015
                     /gene="ribF"
                     /locus_tag="SCM56_001516"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HEG1110297.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            20367..21152
                     /locus_tag="SCM56_001517"
     CDS             20367..21152
                     /locus_tag="SCM56_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG1110298.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            21165..22016
                     /locus_tag="SCM56_001518"
     CDS             21165..22016
                     /locus_tag="SCM56_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HEG1110299.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(22140..22592)
                     /locus_tag="SCM56_001519"
     CDS             complement(22140..22592)
                     /locus_tag="SCM56_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1110300.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
BASE COUNT         7719 a         3957 c         4962 g         6068 t
ORIGIN      
        1 ttaaaaaaag aaaaaattat tcagttttta aataaaaaaa caatgatttc ataggaaaaa
       61 cagttagata ttgctataat acttaagata gtgggaatct tttaagtata ggaggaaaaa
      121 gaatgttacg tttttttccg caaaaaaaca aatatattca agaaaaaagc gaatttcaat
      181 ttaataccga tggaccaatt cctcagcaca ttgccatcat catggacggc aatggtcgtt
      241 gggcgcaaaa ccgtcgtttg ccacgagttg ctggccataa agaaggcatg gaaacagtaa
      301 agaaagtcac aaaaaaagca tcaaggcttg gagtgaaagt gttgacgctt tatgcctttt
      361 cgactgaaaa ctggaagcga ccaaaagatg aagtaagttt tctcatgcaa ctacctgtcg
      421 acttctttga tacgttcgta ccagaattga ttaaagaaaa tgtcaaagtt catgtcatgg
      481 gttatgaaaa cgtattgcca gaacatacgc aagatgctgt aagacgtgca attgagcaaa
      541 caaaaaacaa tactggaatg gtattgaatt ttgctttgaa ttatggaagc agagcagaga
      601 tcgtgactgc tgtccaagaa attgccgaag aagtagcaaa aggagaaata cacgcagaag
      661 aaatcgatga tgaattgatt gctaagcatt taatgacagg tttcttgcct aaagagttac
      721 aagatcctga gctgatgatc cgtacaagtg gagaagagcg aattagtaat tttctgttat
      781 ggcagatcgc atatagtgag ctgtatttta caaaagcgct ttggcctgat tttgatggtg
      841 cgcatcttga agaagcaatc gcctcttatc aaaatcgaga ccgtcgattc ggcggtgtaa
      901 aaaaaacaga agatgagaaa ggggatcagt catgaaacaa cgtgtggtca cagcagccgt
      961 ggcgctagtc ttgtttattc cgattatatg gatcggaggc atagcggttg agcttacagc
     1021 agcaatctta gcagtaatag gagtttacga gctgtttcgg atgaaaggat tgacactctt
     1081 aagttttgaa ggagttttat ccgccgttgg tgcagtgtta cttgttttgc caaaagaaag
     1141 atggtttttc tttcttccag agaatgtaag tacatttatg ttattttatc ttatagttat
     1201 gattctttta ggaacatcag ttatttctaa aaatacgtat acgattgatg aagcaggttt
     1261 tcctgtgctt gtcagcttgt atgtgggaat aggtttccag aattttgtca atgcacgaac
     1321 agaaagcttg gctgttttga tatttggtct atttattgtt tgggcaactg atatcggagc
     1381 atatatggtt ggtcgacgtt atggtcagca taagttaagt cctgatattt cgcccaacaa
     1441 aacgattgaa ggtgcgctcg gtggtattgg tagtgctgtt gtagtcgcat ttctttattt
     1501 attgctatac ccagcaaaag aactatttaa tcatggtatg tttgtcatgc ttctgatgac
     1561 gattattttt tcagcagtcg gacaatttgg tgatttggtc gaatctgcaa tcaaacgtca
     1621 ttatgatgtg aaagactcgg gaaatatctt accaggccat ggtggaatct tagaccgttt
     1681 cgacagtttg ctgtttgtat ttcctatcat gcatttattt ggcattattt agtaagtata
     1741 cctgcgtttc tttgtgaatt tcataaaaga cgcaggtttt tatttttcat tatattgata
     1801 ctattggtgt ctttggtata ctatggtaaa attatagtgt tttatcgttg ttttataaaa
     1861 aacacaaaaa acaagtaaag gagaaaaaag ctatgaaaac gattctgaca tttatcatcg
     1921 tttttggtat attagtgatt gttcatgagt ttggtcattt cttctttgcg aaacgatcag
     1981 gaatcctcgt tcgcgaattt gctatcggta tgggtccgaa aatttatggg catcaagcaa
     2041 aagacgggac aacatatacg cttcgcctat tacctattgg cggttatgtt cgaatggcag
     2101 gcaatgggga tgatgaaaca gaaatggcac caggaatgcc tctttctctt ttactaaatt
     2161 ctgacggcat cgtagagaag ataaatttaa gtaagaaaat ccaattgaca aatgctatcc
     2221 cgatggaatt gagccgttat gatttagaag atgagctgac tatcactggt tatgtgaatg
     2281 gagacgaaac agaagtagta acctaccctg ttgatcacga tgcgacgatt attgaaaacg
     2341 acggaacaga aatcagaatt gcgccaaaag acgtacagtt ccaatcggct aaattgtggc
     2401 agcgtatgct gaccaacttt gctggaccca tgaataactt tattttggca atcctgttat
     2461 tcatcattct ggcatttatg caaggtggtg ttcaagtgac gaacactaat cgcgtaggag
     2521 agatcatgcc taatggagct gcggcagaag caggcttaaa agaaaatgat gaggtagtca
     2581 gtgtggacgg aaaagaaatc cattcatgga atgatctgac aacagtcatt acgaaaaatc
     2641 cggataaaac attagacttt aagatcgaac gagaaggtca ggtgcaatct gtcgacgtga
     2701 caccgaaatc tgtagagtca aatggcgaaa aagttggcca acttggtata aaagccccaa
     2761 tgaatacagg ttttatggat aagatcattg gagggacgcg tcaagctttc agcggttcat
     2821 tggaaatttt caaagcctta ggatcactat ttacaggttt cagtttagat aaattaggcg
     2881 gacctgtcat gatgtaccaa ttgtcttcag aagcagcaaa tcaaggtgta acaactgtta
     2941 ttagtttgat ggcgcttctt tcaatgaatc tcggaatcgt caatctgctt ccgattcctg
     3001 ccttagatgg cgggaaatta gtcttgaata tttttgaagg gattcgcggg aaaccattaa
     3061 gtcaagaaaa agaaggaata ttgacattag ctggctttgg atttttaatg ttgttgatgg
     3121 tgctagttac atggaacgat attcaacgat tctttttcta gaaaaagttg acaaataggg
     3181 tataataaca aacgataact attttgagga aataaaggag tatatagaat gagacaatca
     3241 aaaatgttga ttccaacatt acgcgaagtt ccaaatgatg cagaagtatt aagccatcaa
     3301 atcttgctaa gagctggata tatccgccaa gtatcagcgg gtatttattc atatctgcca
     3361 ttagcaaatc gtgtattgga aaaactgaaa cgcattatgc gggaagaatt tgaaaaaatc
     3421 ggagcggtag aaatgctgat gcctgccatc ttgccagcag aactttggga agaatccggg
     3481 cgttacgaaa catatggacc aaacttgtat cgtttcaaag accgcaatga tcgaaaaatg
     3541 atccttggac caactcacga agaaactttt actgatttga tgcgtaatga aatcaattca
     3601 tataagaaat tacctttaaa tctttatcaa atccaaccta aatatcgtga cgaaaaacga
     3661 cctcgcttcg gtttgcttcg cggaagagag tttatcatga aagatgcgta ttctttccat
     3721 gcaacggaag aaagtttgga cgagacatat aaagattacg aaaaagccta caccgaagta
     3781 ttcaagcgtt gtggcttaga atttcgctcg attatcggag acggtggcgc aatgggcggg
     3841 aaagactcaa aagaattcat ggcgatctca gaaatcgggg aggatacgat ctgttattcg
     3901 actgaaagtg attatgctgc aaacttggag atggctacaa gctactatgt atcaaaaaaa
     3961 tcgcatgaaa cacagcttga attggagaaa gtagctacac ctaacgtaaa atcaattgac
     4021 gaagtagcag atttcttttc tgttgaacct caaaaaatca tcaagtctgt attgttcatt
     4081 gcggatgaac aacctgtcct tgttttggta cgcggagacc atgaagtcaa tgacgtaaaa
     4141 ctgaaaaact ttttatctgc tgacttttta gaagaagcaa cagaagaaga agcacaaaaa
     4201 tatctaggtg ctgagtttgg ttctgtcgga ccagtcggtg tttcagatga cgtaaaagta
     4261 tatgcagacc gttatgtcca agatttagcg aatgccatta ctggagcgaa cgaaacagga
     4321 ttccactata aaaatgtgaa tccagaacgc gatttcactg tactttctta tgaagatctt
     4381 cgttttgtac aagaaggaga tccttcacca gacaacaatg gtgttcttgc ctttactcga
     4441 ggaatcgaaa tcggacatat cttcaaaatc ggcactcgtt acagtgaatc tatgggagca
     4501 actgtactag acgaaaatgg acgagaaaaa tttgtgatta tgggctgtta tggtatcggt
     4561 gtaagtcgtt tattatcagc aatcgtagaa caacattcag atgaacaagg aatcaactgg
     4621 ccaaaaggaa ttgcgccatt tgatttgcat atcgtccaaa tgaatctaaa agacgaatac
     4681 caaacaaacc tgacgaatga actagaagaa gcgatgacaa aagctggcta tgaagtactg
     4741 gttgatgaca gaaatgaacg cgcaggagtg aaattcgcgg attctgattt gattggctgt
     4801 ccaatcagaa tcactgtcgg taaaaaagca gtggatggaa tcgtagaagt gaaaatcaag
     4861 aaaactggcg aaatggttga agtacgtaaa gaagaattgg tgaatacttt accaatcctc
     4921 ttgaaccaag gcgaataata agcgttaaaa cacagaaaat aaaaatagct gaatcagctg
     4981 tttttgacaa tctgatgatt cgcttttaaa attggaactg cgatttgaat cgcagttcca
     5041 attttttaat gtaaaactca gaaaaaacga gtatactaaa aggtactgaa aaagaagagg
     5101 ataagatgcg atcaattgca ttgtttctca tcagaaaggg cgtatatctt tgtcagaaaa
     5161 acgagattta tttgaaaaac tgttggaaca aatccaacta gaggaagcag aaaaaaatca
     5221 tccactgctt actgctggtg aaatggatag tgtagtggtc catcgtaaat cgcgtttatg
     5281 ggaatttacg ctgcattttt ccaaaatttt gcctattatg ctatatcggt cattgactca
     5341 gcatctgact atcgctttta aagatattgc acaagtcaaa ctgaatatct tagcagatga
     5401 ccagacattc gatgaacagc tcttgcaaga ttactgggcc caagctttgg aaaatcagca
     5461 atgcgataca ccactagcac agcaagtatt gaaaacgcag gtacctgtga taaaagatcg
     5521 taaagtcatc ttgccaatcg acagtacagg agctatttct tatttgaagc aacaatactt
     5581 gccattagta gaagagttgt tcgtcagtta cggattccca aaatttcgta tcgaaccaga
     5641 agtggatgag cagcaagcgg aacgagtatt gaaacttttt gaagaacgca aacaagaaca
     5701 ggcagaagcc tttatgaagc aagcagctga aagtctagtt gtccacgaac aaaagaaaaa
     5761 ggaaagaaaa gagcaatcgc cagctcttga aggacccatc cacatccagc taggacgaaa
     5821 tattccagcc gatgaaccaa cgacaccaat gatcagtatc gttgaagaag aacgaagagt
     5881 cacacttgag gggtacgtat tcgacaaaga agtcagagaa ctacgttcaa aacgcaagat
     5941 cttgactttg aaaatcacag attatacttc atcatttatc gtcaaaaaat tctcgaacgg
     6001 cgaaaaagat gaacaagtat ttgatgcgat cagtgtcggt agctggttaa aggtacgagg
     6061 aagtattcaa gaagatacgt ttgtccgtga tttggtcatg aatgcgcaag atatcataga
     6121 agtcaaacat acacctcgca aagattatgc tcctgaaggg gaaaaacgag tagagcttca
     6181 tgttcatagc aatatgagta cgatggatgc aacaaacagc atctctgatc ttgtggcaca
     6241 agcagggaaa tggggacacc gagctatcgc cattacagac cacggtggtg cgcaagcctt
     6301 tccggaagca catagtgctg gaaaaaaagc aggtgtgaaa atcttatacg gcgtggaagc
     6361 gaatgtcgta gatgatggtg tcccaattgc ttataatgat gcacatgaag cattgagtga
     6421 agccacatat gtcgtatttg acgtggaaac gacaggattg tctgcagtat atgacacgat
     6481 catcgagcta gccgcagtca aaatgtataa aggaaatgtg atcgaaagct ttgatgaatt
     6541 tatcgatcca ggacatcctt tgtcacgaac aacggtagat ctgactggta tcacggatgg
     6601 aatggttcga ggatcaaagt cggaagaaga agtattgcgg atgttccttg aattttcaaa
     6661 agatacaatt ttggtagccc acaacgcggc ttttgatatg gggttcttga atactagcta
     6721 cgcaaggtat ggtattcctg aagcggccaa tccagtcatt gatacgttgg aattagctag
     6781 atatctgtat cctcaattca aacgtttcgg attaggtgtg ttatctaaaa aatttggtgt
     6841 cagcttggaa cagcatcacc gagcaatcta tgatgctgaa gcaactggtc acttagcatg
     6901 gatctttgtg aaagaggcaa tggacaatca taacatgctt tatcatgatc agctaaatga
     6961 gcatatcgga gaaggagatt cttacaaaag ggctcgacct ttccatgtaa cgattttagc
     7021 aaaaaatcag gcaggactga aagatttgtt caaactgatt tcaatgtcga atgtcgaata
     7081 cttcgaacgc gtcccacgaa taccacgctc ccaattgaaa aagatgcgtg aaaatctatt
     7141 gatcggctca gcttgtgata agggagaaat attcgaagcc atgatgcaaa aaggggtaga
     7201 agaggcaaga aaccgagcaa aattctacga ttatattgaa gttatgccaa aagcggtcta
     7261 tgcgcctttg atcgagcaag aattggtaaa aaatgaacat gatttggaag aaattatcca
     7321 aaatttggta gaaatcggaa aaagtttgga taaaatcgtt gttgcaacag gtaatgtaca
     7381 ttatctgaac gaagaagatg ccatttaccg aaaaattttg atcaattcta tgggcggagc
     7441 gaatccgttg aatcgtcata gtttgccgga tgtccatttc cgaacaaccg atgagatgct
     7501 aactgcattt cactttttag gggaagaaac agcgaaagag atcgtagtag aaaatacgaa
     7561 taaaatcgca gatatctgcg aagaagtgat tccagtcaaa gatgaacttt atacaccgaa
     7621 gattccaggt tcagaagatg aaatatcaga attgagttac acaaaagcaa aacaaatgta
     7681 cggagatccg ttacctgaga tcattcaaaa acgattaaaa aaagagttga attctattaa
     7741 tggtaatggc ttctcggtta tttatctgat cgcccaaaaa ctagtgcata aaagtaatga
     7801 agatggctat ctagtaggtt cacgtggctc tgttggctct agctttgttg cgacgatgac
     7861 aggaatcact gaagttaatc cgttagcccc tcattattat tgtccagagt gccaatattc
     7921 cgagtttttt gaagatggta cgtatggttc tggatttgac atgccagaaa aacaatgtcc
     7981 aaaatgtggt gcacgcttaa acaaagacgg tcatgatatt ccatttgaga catttttagg
     8041 attccatgga gacaaagtgc ccgatattga tttgaacttc tcaggtgatt atcaagcaga
     8101 agcccataac tatacgaaag tattgtttgg cgaagactat gtttaccgtg ccggaacgat
     8161 tggtacagtc gccgacaaaa cagcatatgg ctatgtaaaa ggatacgaaa gggacaataa
     8221 tctgcagttt cgaagtgcag aagtcgacag attagcaaaa ggcgctactg gagtcaaacg
     8281 gacaaccggc cagcatccag gggggatcat cgttattccc gattatatgg atgtctatga
     8341 ttttacgccg atccaatatc cagcagacga tcaaaattcg gaatggaaaa cgactcattt
     8401 tgattttcac tcgattcacg ataatgtatt gaaattagat attctagggc atgatgaccc
     8461 aactgttatt cggatgcttc aagatttatc agggatcgat ccacaaacga tccctacgga
     8521 tgacccggaa gtgatgcgga tatttgccgg tcctgaagta cttggagtaa gtcaggaaca
     8581 aatttattcc aaaacaggga cattaggtat tccagaattc ggcactcgtt ttgttcgagg
     8641 gatgctagaa gaaacgcatc ctacaacttt tgcagaacta ctgcaaattt ccggactatc
     8701 tcatggtaca gacgtatggt taggaaatgc ggaagaattg attcgacgag gagatgcgac
     8761 actggcggaa gtaatcggct gtcgtgatga tattatggtt tatttgatcc atgctggatt
     8821 agatagcggg atggcattta aaatcatgga aactgttcgt aaaggacaat ggaacaagat
     8881 ccctgatgaa ttgcgagaaa cctatctatc ggcgatgaaa gaaaataatg tgcccgactg
     8941 gtatatcgat tcttgttcaa aaatcaaata tatgtttccg aaagcccatg ctgcagctta
     9001 cgtattgatg gctcttcgtg tcgcttattt caaggtttat ttccctattt tgtattactg
     9061 tgcatacttt tctgtacgtg cagacgactt tgatctagtc tccatgtgca aaggaaaaga
     9121 tgctgtgaaa caagcaatga aagaaatcac ggacaaagga ttagatgctt ccgtaaaaga
     9181 aaagaatcaa ctgacagtgc tggagttagc caatgaaatg ctggaacgag gattcaaatt
     9241 tggaatgatt gatttgtata aatcggatgc cgttaacttt gttattgaag gtgatacgtt
     9301 gatcgcacca tttagagctg tcccaagttt gggaaccaac gtggcaaaac aaatcgttga
     9361 agcaagaaaa gacggtccat ttttatccaa agaagatctt gccacccgcg gtaaagtttc
     9421 aaaaacattg atcgaatata tgaacgacaa tggcgtattg aaagatctac cagatgaaaa
     9481 tcaattatcg ttattcgata tgctataagc atctgttttc ctgtctgctt gtaattttaa
     9541 gcagagtatg gtatagttct tataggtaat gatactaact gtgagtgagc ggatttttcg
     9601 ctcactcttt ttaatggaat tacgcactta attttttaag gaggcgaaac ttttgagtag
     9661 cgtcgttgaa acagtcacag aaatggtgac accgatttta gatgaacaaa attttgaact
     9721 tgtcgaagtc gaatttgtaa aagaaggaaa aagctggttc ttacgagtat ttattgacaa
     9781 agaaggcggt atcgatatcg aagaatgcgc ctttgtcagt gagaaactga gtgaaaaact
     9841 tgatgcagca gatccagatc cgattcctca agcttatttc ctggaggtat cctccccggg
     9901 agcagagcgg ccattgaaaa aagaaagcga ctatgaaaag gcacttggtg attatgtcca
     9961 tgtctcctta taccaaccag tagatggcga aaaacaatat gaaggatttc ttcaatcatt
    10021 tgatgctgaa cagcttacat tgaaaatacg cataaaaaca cgagaaaaag aattgacttt
    10081 tgaccgaaaa aatattgcaa aagctcgctt agctatccaa ttttaatcac ggagaggaat
    10141 cagaaaaaat gagtaaagaa atgttaaatg cgttagacgc tttagaagct gaaaaaggga
    10201 tctcaaaaga aatcgtgatc gatgctttag aagcagcatt ggtttctgca tacaaacgtc
    10261 actatggcca agcacaaaac gtcgaagtgg aatttgatca gaaaaaagga aagatccatg
    10321 tctatgcggt aaaagaagta acggaagaag tgatggattc acagttggaa gtctcattga
    10381 aagatgcact tttaatcaat ccagcatatg agatcggtga tacgatccgt tttgaagtaa
    10441 caccaaaaga ttttgggcgg atcgctgcgc aaacggctaa acaagttatt ttacagcgtg
    10501 tacgtgaagc agaaagaacg attatttata acgaattcag tgcttatgaa aaagacatca
    10561 tgcaaggaat cgtcgaacgc caggataaac gttatattta tgtcaatctt ggtaaaattg
    10621 aggcagttct ttctaaacaa gatcaaatgc caaatgaatt ttatcaacca catgatcgaa
    10681 tcaaagttta tgtttcccga gtagaaaata cgtctaaagg tccgcaagta tttgttagcc
    10741 gcagccatcc agacctattg cgccgcttat ttgagcaaga agtaccagaa gtctatgatg
    10801 gaatcgttga aattgtcagc atcgcccgtg aagctggcga tcgttcaaaa gtggcagttc
    10861 gctcaaatga tccaaatatc gatgcagtcg gtacatgtgt cgggccaaag ggacaacgag
    10921 ttcaagcaat cgtcaatgaa ttaaaaggcg aaaacatgga tatcgtcgaa tggaatgaag
    10981 atccagctgt attcattgca aatgcattga atcctgcaca agtcatggat gtgatcttcg
    11041 atgagacaaa tccaaaggcc tgcacagttg tcgttccaga ctaccaatta tcgctagcaa
    11101 tcggtaaacg cggacaaaac gcacgtttag ctgctaaatt gactaaccat aaaatcgata
    11161 tcaaatcaga atctgacatg acggaatttt atgaaaatca agaacaacaa aaagaaacag
    11221 aagaactgca tgatgaagca atcgttcagt cagatttaac agacgatgaa tatgaaacaa
    11281 ttgcattcaa caatgaaacg gtcgaagaaa aaccagaagc ttaactttag taaggaggca
    11341 aagagatgaa acaaaggaaa attcctttac gcaaatccgt ggtttcaggt gaaatgaaac
    11401 ctaaaaaaga gatggtccga atcacccgtt caaaagaagg ggaagtatcg atcgatccaa
    11461 ctggaaaaat gcctggacga ggagcgtatg tctcacttga gccggaagaa gtacaacaag
    11521 cttgggataa aaaaattctc gatcgagtac tcgaaacaac attaacagat gagttttatc
    11581 aagaacttct tgactatgtc actcaccaaa aagcacgtaa agagttattt ggaaaatgaa
    11641 tacagttaat cggcaaaaag ccatgaatct catcggtcta gcgatgcgcg cgggaaaact
    11701 aatcacgggt gaagaactga ccattgctga aatccgcaaa caaaaagcaa aattggtgtt
    11761 tgtggcaacc gatgcaagcg agaatacaag aaaaaagata aaagataaga gttcatatta
    11821 tgaagttcct tgctttgagc tattttctga agcagaaatc acgcagatga tcggaaaacc
    11881 tcgcaaggta ttcggaataa tggatgccgg atttgcgaag aagaccaagg agctaattga
    11941 aggttaggaa ggtgattgca tgggcaacaa aagaatctat gaattagcaa aagaactcaa
    12001 aaaatctagt aaagacgtcg tggaaaaagc acaacaactt ggcatcgatg tgaaaaacca
    12061 tatgggtgcg attactagca atgatgagaa gaaattgcaa caagcgttca aaaacccaag
    12121 cacgaaacaa caagctgcac aacaagcaag cagaaaacca gccaatcaac agccgaacga
    12181 tcaacgtaaa aaagaaacaa cgaacaatca acaaaaaaat aaaaataacc gcagttatca
    12241 agatcgcggt caagggagcg gtcaagtgaa tcaaggaaaa aaaccaacaa caaaccaaaa
    12301 taatacggga aataccagca gccaaaatcg tcaaggaacg agccaaggcg gcaatcaaaa
    12361 ccgtactaac aataacaaca aaccaaacaa taacaatcgc ggcaaattta ataataataa
    12421 ccgtaatcgt tataataaaa aaggtaaaaa aggcaaacag caacaatcaa ctaaaccagc
    12481 tgtaccacca cgcaaattcc gtgaattgcc agatgtatta gagtatactg aaggtatgaa
    12541 cgtagcagat attgcgaaaa aaattcatcg tgagccagca gaaatcatta aaaaattatt
    12601 tatgcttggt gtcatggtca atcaaaacca agcactggat aaagatacga ttgaactgtt
    12661 agcgacggac tatggcatgg aaccacaaga aaaagtccaa gttgacattg ctgatatcga
    12721 taaattcttc gaggcagaag aagtcgatcc agataaatta gtctcacgtc cgccagtcgt
    12781 aacgattatg ggacacgtag accatgggaa aacaacttta ttggatacat tacgccattc
    12841 acgtgtaaca agcggcgaag ccggcggaat cacacagcat atcggtgcct accagatcga
    12901 tattgatggt aaaccaatca ctttcttgga tacaccagga catgcggcct ttacaagtat
    12961 gcgtgcacgt ggagcaagta tcacagatat cacgatctta gttgttgctg cagatgatgg
    13021 cgtcatgcca caaacagttg aagcaatcaa ccatgccaaa gctgcaggcg ttccaatcat
    13081 cgtcgctgta aataaaattg ataaaccagg tgcaaaccct caacatgtta tgcaagaatt
    13141 aagtgagtat gagttaattc ctgaagcatg gggtggagaa acgatctttg ttgagatctc
    13201 agcgaaattc ggccaaaaca tcgaagaatt gctggaaatg atcctgttag tcgcagaagt
    13261 agaagattta aaagcagatc caacacaacg cgcaatcgga acagtgatcg aagcacgtct
    13321 tgataaaggt aaaggtcctg tgtcaacctt acttgtccaa gaaggaacac ttcatgttgg
    13381 tgacccaatc gttgtcggta atacttacgg acgtgtccgt gtaatgacaa acgatcttgg
    13441 ccgtcgcgat aaagaagcag gacctgcaac gccagtagaa atcactggct tgaatgatgt
    13501 acctcaagca ggtgaccgtt tcgtagtctt cgaagacgaa aagacagcac gtgcagcggg
    13561 tgaagaacgc gcgaaacgtg cattattaga aaaccgtgct gccacaagcc gtgtgacttt
    13621 ggataactta tttgaaagcc tgaaagaagg cgaattgaaa gaagttaacg taatcatcaa
    13681 agcagatgta caaggttcag cagaagcact ggctgcatca ttgaagaaga ttgatgtgga
    13741 aggtgtccgt gtaaaaatcg tccattcagc tgtcggagca atcaatgaaa gtgatgtaac
    13801 cttagcagca gccagcaacg ctattatcat cggtttcaac gttcgtccta caccgcaggc
    13861 aaaagtgcaa gctgatactg aagaagtcga tattcgtctt caccgtatca tctataaagc
    13921 catcgaagaa atcgaaactg cgatgaaagg gatgctagac cctgaatttg aagaaaaaat
    13981 caccggtcaa atgatcgttc gtgaaacctt caaagtttct aaagttggta cgattgcagg
    14041 tgcctatgta acagaaggat atattcgtag agatagcggt gtccgtgtaa tccgtgacgg
    14101 aatcgtaatc tatgaaggcc aacttgcaag tctgaaacga ttcaaagatg acgtgaaaga
    14161 agtcaaaatg ggttatgaat gtggtgcaat gattgaaaaa ttcaatgaca tcaaagtaga
    14221 tgacgtaatt gaaggattta tcatggaaga agtcaaaaat gactaaaaac tgataaaagg
    14281 aggccaatca ttatggctaa ctaccgtgac cgccgagtcg gtcaagaaat cttaaaagaa
    14341 gtcaatgata ttttgcgcaa aaaggtccgt gaccctcgtg tggagaacgt aacgatcaca
    14401 gatgtgaaag ttacggggga cttacagcaa gcaacgatct attacagcat tttatctgac
    14461 ttagcatcag ataagaaaac agcacaagat ggattgaata aagcatcagg attgattcgt
    14521 cgagagttag ggcacaatct aagtatttat aagacacctg aattgttctt tgaacttgat
    14581 gaatcagttg tctatggcaa ccatatcgat gaattgcttc gcgacttgaa caaagactaa
    14641 tcaaaaactc ccgcaaatcc tgtgaaatca ggggttgcgg gatttttttg ttttcattaa
    14701 gacgtattac ctaagagttc catgacttta gtcttgcggc gtgtcagcta accaaaaaag
    14761 agaaaaagaa aagtaaaaaa ttcgactttc ttttttctag tagtatataa atttttgcat
    14821 atagataaag atcaagatac cgataacgga agctagattg taatctattt tagtgacagt
    14881 caggtatagc caagtaacta gaagatcagt ggaacctgca ccataaaagt ttgagtttgt
    14941 cggtccgttg acagttaaaa gaaaaatcac gttgaagtta ttgatatttc cgattacgcc
    15001 gcttcgattt ggtcacgatc caaattttgg ataatccctg tagaaattag catagtagct
    15061 gggataccaa ttcacatatt gacaaagata acagagactt ttacataaaa cggatcaatc
    15121 aggaaaggaa ttgctcgatc cagccccagt tttccagcaa tgtgttaact ggcccagcgt
    15181 cattcagcaa attatgcatg atgaggagcg aaatgaactg cggaacagcc attgtgataa
    15241 cgacaagttt tttggatcaa tgtaaaaaac gtcagcgaaa ggaaaaattt cgtggttttt
    15301 tatacctgtt catttggcta gatataaaat ccaaacaaat gaacagaagt gcttattagg
    15361 tttcaattgg gagtatctgc gctagaataa gagtaataat gaaaaggagt gttatgtatg
    15421 gattattcta gtaaagaata ctttgataaa atgacggctt ggtggcgtgc tgctaattat
    15481 ctttctgttg ggcaacttta tcttaaagat aaccccttac tgagacgtac actgaaaccc
    15541 gaagatgtca aaaaacatcc aatcggtcat tggggaacga ttcctggcca aaattttatt
    15601 tatgtccatt tgaaccgagt catcaataaa tatgatttga acatgtttta tattgaaggg
    15661 ccaggacacg gcggacaagt catggtatcg aatgcttatt tagatggaag ttacacagag
    15721 atctatccag aagtaacgga agatgagaca ggaatgcaaa aattattcaa acgattttca
    15781 tttcctggag gcattgcttc tcatgcagca ccagaaacac caggttctat ccatgaaggc
    15841 ggagagcttg gatactctct ttcacatgca gtcggagctg tattagacaa tcctgaagta
    15901 atctctgctg tcgtgattgg tgatggtgaa gcagaaaccg gtcctttagc tggaagctgg
    15961 ttctcaaatg tctttatcaa tccagtgata gatggagctg ttttaccgat ccttcattta
    16021 aatggtgcaa aaatagccaa cccaacgatc ttggcacgta aatcagatgg agaattagca
    16081 aattacttta acggacttgg ctgggaacct ttctttattg aaggaaatga ttcagaaaaa
    16141 ttaaatcctg ttatggctga aaaaatggat caagcaatcg agaaaattaa atccatccaa
    16201 aaagaagctc gcctgaagac agcaacagat gtcgtgatgc caaaatggcc agtattgatc
    16261 gtacgcacac caaaaggatg gaccggtcca aaagaatggg acggggaacc aattgaagga
    16321 accttccgag cacaccaagt accgattcct gtagatcaag aacatatgga tcatgcagat
    16381 gctttactcc gttggctgaa atcctatgaa ccagaaaaat tatttgatgc acaaggacgt
    16441 attttagaag aaatcagaga aatcgcgcct actggtgacc aaagaatggc aaaaaatcct
    16501 atcaccaatg gtggaatcga tccaagaccg cttattatgc cagattggaa aaaatatact
    16561 ttgcaatttg aaaaaccagg ctccgttaaa gcagaagata tgaccgaact tggtaaattc
    16621 gttcgcgaga tcattgagaa aaatccagaa aatttccgta tatttggacc agatgaaaca
    16681 aaatcaaacc gattgaatca agttttcaaa acgacaaatc gccaatggat ggaaaaaatc
    16741 gaaccagaaa atgatgaatg gctttcgcca tctggtcgcg tcatcgacag tcaattatcg
    16801 gaacaccaag atgaagggtt cttagaaggg tacgttctga ctggacggca tggttttttg
    16861 caagttacga atcattcttg cgagtggttg actctatgtt gactcagcac ttcaaatgga
    16921 tgcggaaatc acatgacctt tcttggcgaa atgattatcc ttcgctaaat ctcattgcaa
    16981 gctcaacagt cttccagcaa gatcacaatg gatatagcca ccaagatcca ggaatattga
    17041 cacatttggc agaaaagaag gcggagttca tccgtgaata tctgcctgcg gatgcaaata
    17101 cattgctcgc agtcatggat aaagcattcc gctcgtcaga aaaaatcaat ttgatcatct
    17161 cttccaaaca tccgcgtgct caattttact cagcagaaga agcagccgtc ttagtgaatg
    17221 aaggcttgaa aatcattgat tgggcttcga cagcaaaaga ggaggaacct gaattggtca
    17281 ttgctgctgc aggaacggaa tctaacttag aagcattagc agctgtaact ttgctgctgg
    17341 aagaattccc aaaactaaaa attcggttca tcaatgtcgt agatttattg aaattacgtc
    17401 atccaagtca agatccgcgt gggttgagcg atgaagaatt tgatcaatat tttacaaaag
    17461 acaaaccgat cttatttgct ttccacggtt atgaaacatt ggtccgcacg attttctttg
    17521 atcgtcataa ccatcattta atgattcatg gctacaaaga aaatggagat atcacgacac
    17581 catttgacat gcgtgtagta aatgaacttg atcgttatca tttggcaaaa gacgcagctt
    17641 taaaaatcaa aggaagtcaa gcagaagatt ttgcgaaaaa aatggatcag aaattacaag
    17701 aacatcaaaa ctatattcgt gaaaatggta ttgatctgcc agaagtattg gattggaaat
    17761 ggaaaaatct tgaccaataa aatgataaaa tcgatcccgc aaaatagaat tttttacatt
    17821 gattaagaaa ctgaaaaaat aaataaaaaa aacggaaaaa agactggaag aatggccagt
    17881 cttttttttc gtttttggga agtgaaaacc aaactattta cctgtaagcc acccataaat
    17941 ccaatcaaat ggtgcaaaga aaaaatcttc cattctattc cctcctttaa aaaagcataa
    18001 catgatttct atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
    18061 ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt
    18121 aaatcaaaaa tgaaatggag aaaagacatg gacgggttat tacctttatg gaaagaacgc
    18181 ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc
    18241 ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa
    18301 gccacaaaag ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact
    18361 ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta
    18421 gatatgtctt tatctgaaaa aaaatagatg aagtgatggc ttcatttatt ggtgagatca
    18481 cccagatacc accaatgtac tcagcggtaa aagtcaacgg taagcggtta tatgaatatg
    18541 cccgcaataa tgaaacagtg gagcgtcctg ttagaaaggc tcagatttac cgttttatcc
    18601 ggacaagtga attgacaaga gacgaagtga caggaaccat ttcttggcgc ttcgaagtag
    18661 aatgcgggaa aggaacctat gtccgcacat tagctgtaga tacaggcaag aaacttggtt
    18721 accctgcgca catgtctgat ctgacccgaa cagcaagtgc cggtatgtcc cagaaggatg
    18781 ctctgacatt agaagaagta gctcaagctg tgaaaaatgg gacaatcgat gattgtttgc
    18841 tgccaataga aaaaggagtg gaacggttca aacgagtaga tattactgat gaagtatggc
    18901 agaaagtaaa aaacggtatg agacttgatt atactgtatt tgcgctccca gaaatgccgt
    18961 cagaagaaat tgctttgttt tataaaggaa aagttgtcag tatttatcag ccaaacccga
    19021 aagaaaaaaa caaattaaaa cctagtaaag tattgagaaa tgaggaataa cgaatgaata
    19081 tcatcaagat cagacatcct taccaatcag aacaaattcc agaagacgat gtcgttttag
    19141 tgctgggatt tttcgacggc gtgcatatcg gacaccagaa agtcataaag acaggtatgg
    19201 aaattgcaca aaaagaagga ttgaaattag cattgatgac tttcaatcag catccatcta
    19261 ttgtctttaa aaagatcgat cctagcagcg tgaaatattt gactagcctt aagcaaaaag
    19321 aagaaaaaat ggccgctttg ggtatagatt atttatatga agttgatttt acttcttctt
    19381 ttgctcattt agcaccgcag gaatttgttg atcaatacat cgtagggtta catgcaaaat
    19441 atgctgtttc cggttttgat tatacgtatg gaccaaaaga cattgcagat gtcgctcatt
    19501 ttcctgaata tgccaaagga agattcaaaa tcgtcacagt ttctaaagaa gaagaacaag
    19561 gtgaaaaaat cagctctacc cgtattcgtg gattactaga cagcggacaa gtaaaagaag
    19621 cagcaaaact attaggttgc acatatgagg tagaaggggt agttgtccat ggcgatgcac
    19681 gaggcagact actaggattc cctacagcaa atgtcaaagt aaagagcacg gttcatttgc
    19741 ctaaagaagg tgtttatgtg tcggaaataa aaattgggga aacatggtat ccagccatgg
    19801 gttcaattgg acataatgat acattcggtg aaggacggga gctgactgta gagttgtata
    19861 ttttggactt taatcaagat atctatggtg aacatgtcgc ggtacgctgg aatcaatttt
    19921 taagagatca agtgaaattt tctagtgtag aggaattgat tgaacaactc aaggaggatg
    19981 aacaagcaac agctgaatat ttttctcagc aataaaacat tatgcataaa atctcttgtt
    20041 tatgtataac tagcaattac aataaaatgg gtgataaata agcccaggaa aggggctgtg
    20101 acaaaagtct tgtcacagcc tttttttcca aataaatggt ttttcagaag tagcttcttc
    20161 ggaaataagc cgaaatttct tgaagctgac cacttctgtc acaacctctt tctttataga
    20221 aataaaagga cactagcaat tcatgaaaac gattgctaga gcgtatcatt tgctttagac
    20281 tatatatctt ggtgtacact cactttaagg cggaaataaa agttatccgt aataacaggt
    20341 ttaggtatga aaaaggagtg ggtactatgt acaaggaatt agaaaacaaa gtcgcagtag
    20401 taactggtgg ctcaaagggg attggaacag cgattgctca acgattcggg aaagaaaaaa
    20461 tgaatgtagt tgtcaactat aactcggata aagaaggagc agaaaaagca gtcaaagcaa
    20521 tcgaagaggc aggcggaaaa gcagtctctg tacaagcaga tgtcagttct gaagaaggaa
    20581 tacagcgttt acttgatacg gctttagaaa attttgggac actggacgtt tgggttaata
    20641 atgctgggat ggaaaatcag catccaaccc atgaacttag ccttgaagat tgggaaagag
    20701 tgttaaaagt caatttgact ggcgttttta tcggaacgaa agcggcttta aattacttta
    20761 cagagaataa taaaaaagga aatatcatca atatgtcttc tgttcatgaa cagatcccat
    20821 ggccaacgtt tgctcattac gctgcttcaa aaggcggggt aaaattattt accgaaacgg
    20881 tagcaatgga gtatgcaccg aagggaatca gaatcaatag tataggccct ggtgctatcc
    20941 gtacacctat taatgcggca aaatttgatg atcctgaaca aaaagaactc ttggaaagta
    21001 tgatcccact aggacgcgta ggtgaaccaa aagaaatagc ggcgacagct gcatggttgg
    21061 cttcagacga atccagttat gttacaggta ttacactatt tgcagatggt gggatgactt
    21121 tatatccttc tttccaaggt ggaaaaggat aagggtgaat aaaaatgggt ttattgattg
    21181 cattgatccc ggcagttggc tgggggatcc agccactgat tttgaaaaag attggtggca
    21241 gaccaacaaa tgagatatta ggaactggta ttggagcggt gcttgtcggc ttactcgttc
    21301 aattattcgc ttctccagct ggtatttcag tgactacatt tttgattagt ttactttctg
    21361 gtgcattttg ggttattgga caaacaggac agtatacatc tttcaacttg attggtgttt
    21421 ctaaaacgat gccaatttcg actgcattac agttagtcgg aacctcgttg attggtgtct
    21481 tcgcttttgg agaatggtct ggaagtatgg ggaaaatcat tggagcagca gcaatcattc
    21541 ttcttgtatt tggttctgct ttaacggcaa tcagtgatga tcctggaaaa aaaggcggta
    21601 tcacaaaagg aattagtcta ttagcatcaa caagtattgg ttattgggta tacagtgcat
    21661 tgcctaaact agttgatgca agcggacttt ccattttctt tccgcaaatg atcggtgtct
    21721 ttttaggggc agtgatttat gtgattttcc gtaaaccaac cgcattttct gaagacaaga
    21781 gttggaaagc aacgatcgtt ggagccatct ttagtgtgtc agcattggct tatatttttt
    21841 cagcaaatga aaatggggtt gctactgcat atatcattac acagctaaac gtagttatct
    21901 ctacattagg cggtatgtac atcctgcatg aaaataagac accaaaagaa aaaaaattaa
    21961 cgattatcgg attattgctg atcgtagtag gaagcatggt cacggtcttt atttgaggat
    22021 aaagatggta aaatttcatt tattaaaaaa atatttgttt gaaccaaaca gtaagtactt
    22081 ataaaaaaat agcgctagaa tttccaacat ctctggaatt tctagcgctt tttaattgtt
    22141 taagatacaa gggaaatatc tgtctggatc aataattttt cattgatcca ttgactaaag
    22201 tttacgacat aattgaagtt accaccgatt agtatctccg catgattgtc tttataaaga
    22261 tcgtattgta aaagacggtc ttttttattt gcgtaaacat cgttatggta ctggactaat
    22321 tgttctcgga attgattgat tgctgtaaaa tcacgatatt gaaggttgaa acgcatcgtg
    22381 attggtgcat ttagcatgaa tcgctgcatt tcttttgctg cttcctctgc ttgatctccg
    22441 tataggaact caaaaagatt acgttcagaa aatttccatt gcccgccaac ctttgttcct
    22501 tttagttttc cttcatgaag ataatttctg atagaacgtg tagtaacacc taattgttcg
    22561 gctaattcat ttactgagta aaaatctttc aatgttcacc ctcctatgtg aatggttcta
    22621 ctgacttagt tgatatttgc ttaatcacga aatgtagaaa gaacgatttt aattaaggtc
    22681 attgtaaaaa tcaagtcaga aaattc
//
LOCUS       DARBDV010000042        22187 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000042 DARBDV010000000
VERSION     DARBDV010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.042"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            55..1953
                     /locus_tag="SCM56_001520"
     CDS             55..1953
                     /locus_tag="SCM56_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HEG1110301.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(2252..2971)
                     /locus_tag="SCM56_001521"
     CDS             complement(2252..2971)
                     /locus_tag="SCM56_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEG1110302.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            3308..3640
                     /locus_tag="SCM56_001522"
     CDS             3308..3640
                     /locus_tag="SCM56_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110303.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            3627..5630
                     /locus_tag="SCM56_001523"
     CDS             3627..5630
                     /locus_tag="SCM56_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEG1110304.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            5649..6119
                     /locus_tag="SCM56_001524"
     CDS             5649..6119
                     /locus_tag="SCM56_001524"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEG1110305.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            6140..6724
                     /locus_tag="SCM56_001525"
     CDS             6140..6724
                     /locus_tag="SCM56_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HEG1110306.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            6739..7725
                     /locus_tag="SCM56_001526"
     CDS             6739..7725
                     /locus_tag="SCM56_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEG1110307.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            7715..8026
                     /locus_tag="SCM56_001527"
     CDS             7715..8026
                     /locus_tag="SCM56_001527"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEG1110308.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            8048..9829
                     /locus_tag="SCM56_001528"
     CDS             8048..9829
                     /locus_tag="SCM56_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEG1110309.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            9822..11198
                     /locus_tag="SCM56_001529"
     CDS             9822..11198
                     /locus_tag="SCM56_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEG1110310.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            11205..11840
                     /locus_tag="SCM56_001530"
     CDS             11205..11840
                     /locus_tag="SCM56_001530"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEG1110311.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            11870..13225
                     /locus_tag="SCM56_001531"
     CDS             11870..13225
                     /locus_tag="SCM56_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HEG1110312.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(13417..14811)
                     /locus_tag="SCM56_001532"
     CDS             complement(13417..14811)
                     /locus_tag="SCM56_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEG1110313.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            14903..15874
                     /locus_tag="SCM56_001533"
     CDS             14903..15874
                     /locus_tag="SCM56_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEG1110314.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            15871..16653
                     /locus_tag="SCM56_001534"
     CDS             15871..16653
                     /locus_tag="SCM56_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEG1110315.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(16822..17193)
                     /locus_tag="SCM56_001535"
     CDS             complement(16822..17193)
                     /locus_tag="SCM56_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG1110316.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            17327..17770
                     /locus_tag="SCM56_001536"
     CDS             17327..17770
                     /locus_tag="SCM56_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEG1110317.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(17813..18622)
                     /gene="yidA"
                     /locus_tag="SCM56_001537"
     CDS             complement(17813..18622)
                     /gene="yidA"
                     /locus_tag="SCM56_001537"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEG1110318.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(18641..20008)
                     /locus_tag="SCM56_001538"
     CDS             complement(18641..20008)
                     /locus_tag="SCM56_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEG1110319.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(19992..20822)
                     /locus_tag="SCM56_001539"
     CDS             complement(19992..20822)
                     /locus_tag="SCM56_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEG1110320.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            20985..21422
                     /locus_tag="SCM56_001540"
     CDS             20985..21422
                     /locus_tag="SCM56_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEG1110321.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            21498..22118
                     /locus_tag="SCM56_001541"
     CDS             21498..22118
                     /locus_tag="SCM56_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEG1110322.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT         7117 a         3850 c         4783 g         6437 t
ORIGIN      
        1 atagagcata acaactttat aatgttgagt ataccaattt ttggaggcgt tttaatggac
       61 cgctatttag atttactttc agagaagttc cctacaacgg aagatgtcat cacggaaatc
      121 atcaatctcg aagcaatttt gcagcttcca aaaggaacag agttattttt gagcgatata
      181 catggcgaat ttcctgcttt tgatcatatt ctgcgtatcg gttcaggaaa tcttaaagaa
      241 aagatcaaag accttttctc tgaacaaatg acggaaacgg agatcagcca attcactatt
      301 tttacggctt atccagaata tgtgttgacg actgattggt acaaaaaaca agataaatcc
      361 cagctgatcc atcgtttgat cgatctttta cggtttacga cagtcaaata cactcgatca
      421 aaagttcgaa aaggattgcc gaaagaatat agctatatca ttgaggaact cttgtatatc
      481 gatgatcgta tcaacgggaa aaaagactat gtaaaaaaaa tcattgaaca acttgtcttg
      541 atgaaggaag aagaaaaatt tttaacaaaa cttgcgcaga cgatccaaaa atctgtcatc
      601 gatcatttgc atatcgtggg tgatattttt gatcgaggaa ctcaagcagc caaagtaatg
      661 gatcgaatca tggatttttc ctctgtagat atccagtggg ggaatcatga tattttgtgg
      721 attggtgcgt attgcggctc tgaggcatgt ttattgacgc ttttacggat tgccgcacgc
      781 tacaactatt tatttgaatt agagaaagaa tatggattaa atttacgtcc attattttct
      841 tttgcccatc aaacctatca gaagaatcca gtatttaccc ctaaaaacag gaaagatcta
      901 tctgaaagag agcaagaaga attagaaaag atccatcaag cactttctat cctccaattc
      961 aaatcagaac atcagttgtt gaatcgacga cctgaattta aaatggacga tcgtaaattg
     1021 ttggaaaaaa tcaactatga ggaatcaacg atcgttttag aaggtcagct ctatgagttg
     1081 aaagggactt gttttcaaac gatccagcca gatgatccta aaaaattctt ggaagaggaa
     1141 caaaaagtga tcgatagttt gatgtattca ttccagcact cgttaagaat gcaaaaacac
     1201 atgacgtttt tgatcgaaaa agggggaatg tatctcacga acaatcagca tgtactgttc
     1261 catggctgta tacctgtaga tgaaaagggg cagctcttag catttgaatt agaccaatct
     1321 tatcgcggaa aagaactgct gggatttttt gaaaaacaga taactgaaag cgcaaaagat
     1381 ttgactgcaa atgaagattt ggcgaccgat ttgatttggt atgcgtggag cgggccattt
     1441 tcgcctttat tcggcaagag taaaatggca acttttgagc gctattttct cacagagcct
     1501 catacgcacg tcgaaaaaag caatccctat tatcacttgc gagatgaaga gtggttcagt
     1561 gaaaaaattt tggcagaatt cgatgcaaaa gaagaaggtt cagcaatcat caatggacac
     1621 acgccagtaa aagtaaaaaa aggagagtca ccgatcaaag caaacggaaa agcatttgtt
     1681 attgacggag gtctatccaa agcttatcaa aaaacaaccg ggattgctgg ctactcatta
     1741 ctatgcaatt cttacggttt ccaaatcgtg acacattatc cgttcttgcc tattgaacaa
     1801 caatttgcaa aaaaatctga tcaaacgaat gtaaaaaaag tgatcgagca gcagctccca
     1861 cgcaaactta atcggaatac gacaaaagga atggaattac agcaacagat tactcaactc
     1921 caaaggctgt tggattatcg aaaggacgat tgagaaaaat gctagccaac attgctcaat
     1981 agactaaaaa gcgactgcaa caaaaggcgt gtcgcagtct ccttcggaat tagctcaaaa
     2041 aacggtaaaa tattagaagc tatttttctg tttatttctc taatactcag gtcagggcgc
     2101 tgatttcacg acctcttcct taaacggtgt tctcctgcca actcctcggt cacaagtcac
     2161 aatatccaac aaacatgaga agctgttttt tgaaattgct ccttgtgact cagagttgaa
     2221 cggcaggctc acgacctaat cagaaaaacg attaaaattc attcacaaag aaatcgaata
     2281 aacgctgttc agatgaatct acacgatgag aaagttccgg atgccattgg acaccaagga
     2341 ttttgtgcat attatcttta ctttggactg cttcaatgag tccatcgttg gaaaaggcga
     2401 tggcttttaa caaaggagaa agatctttga ctgcctgatg atgataggaa ttcacaaagt
     2461 atgaatcagg gagaaggtca cttaataaac tgtccgaaac gattttgact tcgtgagttg
     2521 caaactgcgg ggcagtgggc tgttgttcgt gttttacgga ccattttgga tataatgaaa
     2581 gatcttgata aagtgttccc tccaacacga cattcaatag ttgaagtcct cggcagactg
     2641 caaaaatcgg ttttctttgt ttcaatgctt ccaagatcaa tgcctgttcg aattggtctc
     2701 gttgaatatt tgtttccgtc agttttggat gagggtcttc catgtaaagt tgcggactaa
     2761 catcctgacc gcctgcaaga atgagtttgt cgattttctc aattagttga ggcgctgaat
     2821 ctggagacat gactggcaga ataagaggta atccttgagc atctaaaact gcatccacaa
     2881 atccctgcgg tgtatagctt acttgatttc cttggaaagt atctgtcgct cgaatcagtt
     2941 gattcccggc aatgccgatg attggtttca tttttattcc ccctaaagta gactaattgt
     3001 aacaaggatt taaaaaaatt taaactatgt aagacttgcc cctatactta tctgttcttg
     3061 ctgtgttact atctgtaaag agaattttcg tgtgaaattc atgacgatag ttgaaaatat
     3121 tgcttaattt gttataataa aaagtaagca gaaataaaaa ggttgggcgt aagcttcaag
     3181 gatctagttc ttcaaggggt gagaaggaca ttttattgaa ggggcctgcc tatactaagt
     3241 tatctttaga ggtacatact ttcaatgaaa aaagaaagca tgtacctctt ttttaaatcg
     3301 aaaggaagtg aagcaagtgt tagaagcttt ggaacaaatc cgaaaagcag aagaagaaaa
     3361 tgagaagcgt gctcgaaccc tacggcagca gttgaagacc tatgcagaag aaaaagagca
     3421 agaattggag ataatccaaa cgaacagtcg gcaacagctg aaacagttgg tagaagaaaa
     3481 agaacaattc gaaaagcaag aattagaaaa agaaaaaatg aaattgaaag cagaagcaga
     3541 caaggtgcaa gcaactttga aagaccaata tcaaaagcat cagagtcaag caatcggtgc
     3601 aatcattgaa agggtgaaag aaacttatgg ccgtcactaa gatggaaaaa gtgaccctca
     3661 tttcagataa aagaaatcag gagaggattt tacaagcagt acaaggaatc caaaatgttg
     3721 aaatccggga tctgtatcaa aatagaacaa acaaccagtg ggtagaaaaa tattttccag
     3781 ataccacgat tattgataaa gaagcaaaat taagcgcttt aggcaatcgg ctgaaagaca
     3841 tccaagaagc tatccaattc atacaacacc acggcgatag ccgtcaaaag aaactccatt
     3901 tgaaacgtca agaactttcc ttacaagact tggaagcaaa ttattccgaa gaaaatttca
     3961 tgaataaatt agaagaagtc cttgatttaa aagaaaagtg ggaacagcta actgaaaaac
     4021 gtgatcgttt agaagaagag gaagaatggc ttcttcattg gcaacagctg gatattgctc
     4081 caagtatgta tgcaagtaag acgaccattt ttgagatggg gactgtgagt gcagcaaatt
     4141 ttgaaagttt caaggaagaa ctaagtaagc ttgatgacgt ttatatggaa gaaatagatt
     4201 atacgccaaa acaagtttat ctttcgtatg ttgctttaaa aaaatcagtt gctgctatag
     4261 aagaaattgc tgggaaatac gggttcaata aagaaacgta tccttatgaa caaaatccta
     4321 aagaaaaatt gcaagaaacc aaacagcaat tatccattgt tcacgagcag caaaaaaaga
     4381 taacgacagc gttaggtcag tgcagcggct atatccaaga atttgaatgg gtagaagaag
     4441 tcacacttgc cgttgcagaa cgggaaaaag taaaagaacg gtttatccat gccacttatc
     4501 tgatcgtttt gcaaggttgg gtagatgcag aagaaaaaca gaatttgatg catgtgttaa
     4561 acgaaactgt atcagaagaa gatatatacg ttagtttcga aacaccaaac tcagaagaga
     4621 ttcagcagga agtccctact aaattgaaaa accatccgct tatcgaacct tttgaattgc
     4681 tgactgaaat gtacagtctg ccaaaatatg aagaagtaga tccaacgcct tggatgacac
     4741 cattttatct tgtcttcttc gggatgatgg tcgcagatgt tggttacgga ttgctgatgc
     4801 tcattgggac aatactagct caaaaactat ttgttttacc ccgcgggatg aaacgtttcg
     4861 taaaattctt tgagattctc tcggtaccat cgatcatctg gggattgatc tacagttcat
     4921 ttttcggaca ggcattacca aaagaactat ttggtatccg cttgcctttc ccaatcctat
     4981 ctacgacgga tgacgtcaat acgattttga ttttatccgt gattttcggt ctgatacaga
     5041 tcttggttgg attattcgtg gcagcaaagg aaaatatcag gaggaaagat tacttgagtg
     5101 ctgtcagtga tggttttgcc tggcaaggga tcctgattgg gatcgttatt gcattgattg
     5161 gtgtcatgtt gataaaaaat acagcatttg tttatcttgg cgcggggcta tcgatcattg
     5221 ctgctttatg tatcttaatc gtgccgatta tccaatcgcc gtctaaagtc aagggtgctg
     5281 ccaaaggagc gtataatctt tacggattga ctggttatat cggtgattta gtcagttata
     5341 cacgtttgat ggcactaggt atttcaggcg gaagtattgg cgcggcattc aatatgctag
     5401 ttgctttcat gccgccagcc gctcgattca gtgtagggat ccttttgatc atcgctttac
     5461 acgcactgaa tatgtttttg acactgttaa gtgcctatgt ccatggtgca cgattgcagt
     5521 atgtcgaatt ttttggaaaa ttttatacag gaggcggacg tgcctttgac ccgctgaaaa
     5581 cggcggaaaa atacgtcaac atcaatcata agaaaaaaaa caataaataa ttggaggatt
     5641 tttatacaat gatggattac ttaattagtc aaaatggtgg aatggtattt gcagtattag
     5701 cgatggcaac agcgacgatt ttttcaggga ttggttcagc taaaggtgtt ggaatgacag
     5761 gtgaggcagc agcagcgtta acaacaagtc aacccgaaaa attcggacag gctttgattc
     5821 tgcaattgct tccaggtacg caagggttgt atggattcgt tatcgccttt ttgattttta
     5881 tcaacttagg aaatgatatg tcagttgtac aaggattgaa ctttctaggc gcttctctgc
     5941 cgattgcttt tacaggtttg ttttcaggta tcgcccaagg gaaagttgca gcagcaggta
     6001 tccaaatttt agcgaaaaaa cctgaacatg ctacaaaagg aatcattttt gctgcgatgg
     6061 ttgaaacata tgcgatttta ggtttcgtta tttccttcct attagtattg aatgcctaag
     6121 cataaaggag gaaaagcaga tgaacgccat tgaaaaaatc atctctcaga tgaatgaagc
     6181 agcagagcaa gaaagagcgg cgttagagca agaagagcga atgaagatcg atcaaaactt
     6241 tgaacaaaaa cgtacgcaaa tcgaaacaga acatcaaaaa caaaaagaaa aacaaatcga
     6301 attacttgaa aaaaaatacc gtcagcttag gaatcgccaa caagtagaag tacgacaaga
     6361 aaatttgaat gcgaagcagg aatttttacg acgattgttt gcagatgcaa tagctgaaat
     6421 ggagaattgg gatgaatctg aacaaatcca gtttatcaaa aattcattgt attcattacc
     6481 tttgacaggc aaagtaacat tcattgctgg agaaaaatca gcagcttatc tttctcagac
     6541 attgctggat gaatggaaca atgaacttcc ctttatgatg gttttaagtg atgaaactgt
     6601 tgctgatcaa gcgggttttc tgataaacga tcaaggagtt cagtataatt ttcttttcag
     6661 cagccttgta caagatatcc aaggaacgat gagttttgag attgccaatc aattattcga
     6721 ataaggaggg atcagtgtat ggaataccac gaactaaatc ccttgattcg cggaagagag
     6781 cttgaacttt tgtccaatga gacatttgaa cggatgattc aaacaaattc tgtcgaagca
     6841 cttggtgaaa tattgaaatc cacgatttac agtccatata tttatgacgg atatgaatat
     6901 gacttcgaac agaatctttt aaaggagcag agccgtctat ttcgctggtt aaaggaatca
     6961 gcaccggagc ctgaaatcgt ttggatctat acgatgcgct atactttcca taatctgaag
     7021 gttttgacaa aagccgagat gacaaatcaa aatctggacc atttgtatat cgacgatgga
     7081 ttttattcat tagaaacctt gaaggatgcc attcatacgc aagtttcaaa tgaactccct
     7141 gaaagcatta tggacagtat acgagaagta cgtgattatt ttgaagagtc taccattctt
     7201 cagggaattg acgtgattta tgatcgacag tttttgacag agcagcgacg gcttgccgat
     7261 aagctaggat atcaagaatt gctgacagaa gtcattgctt ttattgactt gaccaatatc
     7321 acaacgatgg ctcgaggaat attgcagaaa agaacatatg ggttcatgac gacggtactt
     7381 tccagctcgg gaagtatcga aaaagaagta tttttgaact ttgtagataa agatatggaa
     7441 gattacacga attttctatt agagacagat tatgctgatt ttctgcgccc tgtcatccgt
     7501 gagggaaatc ttgatctcgt aaagcttgaa cagttgaaag atgattatct aagttcattg
     7561 tatcaagaag cacaaaccca ggctttcgga ccactgccac tactagcctt tttgaacgca
     7621 aaagaggtag aaagcaaaaa ccttcgttta ctggttatcg gcaaaagaag ccatttcgaa
     7681 ccagaaacaa tcagaaagcg ggtgagacaa gtctatgacg cataagatcg gtgtagttgg
     7741 cgacaaagat tctgttttgc cattcaaatt gtttggcttt gatgttcgtt atggtacaac
     7801 caagcaaacc gttcgtcagg caattgaaga aatggcaaag aatgagtatg gcgtgattta
     7861 tgtcacagag caatgtgcag cattgagccc agaaacgatc gaacgttaca aggaacaaat
     7921 gatacctgct atcgtattga ttcccaatca tcaaggatca ttagggatag ggctggaaga
     7981 aattcaaaaa aacgtagaaa aagcagtagg tcaaaatatc ttatagtatg aaaaggagat
     8041 agaaattttg caaattggaa aaatcataaa agtctccgga cctcttgtaa tggctgagaa
     8101 catgtctgat gccagtatcc aagacatgtg tctagtggga gatctaggag ttatcggaga
     8161 aattatcgag atgcgtggcg atgtggcgtc tattcaagta tatgaagaga cttctggaat
     8221 tggtccaggc gaaccggttc gctctactgg tgaagcttta tctgttgaat taggaccagg
     8281 gatcatttca caaatgtttg acggaatcca acggccatta gataccttta tggaaatcac
     8341 acaaagtaat ttcttagggc gcggagttca attgcccgca ttggaccatg agaaaaaatg
     8401 gtggttcgaa ccaacagtag aagccggaga aacggtgagt gctggagatg ttatcgggat
     8461 cgtagaagaa acaaaagtta taaaacataa aatcatggtg ccaaatggga taaaaggaac
     8521 gatcaaaaca atcgaaaaag gttcattcac tattgatgaa acagtatgtg tcattgaaac
     8581 cgaaaatggt gacaaaaatt tgacgatgct tcaaaaatgg ccagtaagac gtagtcggcc
     8641 aatcaaagaa aaattaaacc cggatgcacc catgatcact ggacagcgag tgattgatac
     8701 attcttccca gtgacaaaag gaggagcagc agctgtacct ggtccttttg gtgcagggaa
     8761 aacagtcgtt cagcatcaaa ttgcaaaatg ggccgatgtg gacatggttg tctatgtggg
     8821 atgtggcgag cgtggaaatg agatgacgga tgttttaaat gaattccctg aattgattga
     8881 ccctaataca ggagaatcgt tgatggaacg tacggtattg attgctaata catcaaacat
     8941 gccagtagca gctcgagaag cttctatcta tacgggaatc acaattgcgg aatatttccg
     9001 tgacatgggt tatgacgttg ctatcatggc agactccact tcacgatggg cagaagcact
     9061 tcgtgagatg agcggacgtt tggaagaaat gcctggtgat gaaggttatc cagcttatct
     9121 tggttcacgg cttgcggaat actatgaacg atctggaaga gtcattgcat taggatcaga
     9181 aggtcgcgaa ggaagtataa ccgcaatcag tgctgtatcc ccgtctggag gggatatttc
     9241 ggaaccagtc acacaaaata cattgcgggt cgtaaaagta ttctggggtc ttgattccag
     9301 tttagcccaa aaaagacatt ttccttcgat cgattggata caaagctatt ctctttattc
     9361 aacagaagta gggaagtata tggatcatat ccttcaagct gactgggcat ccatggtcac
     9421 cgagggcatg cgtattttgc aggaagaaca gcagctgaat gaaatcgtgc gtttggtagg
     9481 gattgattcc ctttcagata atgaccgtct gacattagaa gtggcaaaat ccattcgtga
     9541 agattatttg cagcaaaatg cttttgatga tgtcgataca ttcacttcaa gagagaaaca
     9601 attcaaaatg ttgaagatga tcttgacctt tggtgaagaa ggacgccgtg cgttgtcgtt
     9661 aggtgcttat ttaagtgaga tcatggacgg taccgtatcg attcgtgaaa ggatcggacg
     9721 aagcaaatat atcccggaaa cagaattaga aaaactagat caaattattg gtgaaatcaa
     9781 gcaaacgatg caaacgatcg tttcagaagg aggcatgagc aatgattaaa gaatatcgta
     9841 cgatcggtga agtcgtcggt ccattgatgg cggttgaaaa agttgctggt gtcaaatatg
     9901 aagaattaat cgaagtccgt atgcaaaatg gcgagattcg tcgtggacaa gtattggaag
     9961 tccaagaaga taaagcaatg gtccaaatat tcgaaggaac aagtggtatc aatttacgtg
    10021 actcttctgt acgattttta ggccatcctt tagaactagg tgtatcggaa gacatgatcg
    10081 gtcgagtatt cgatggtttg ggacgaccga aagataacgg accagagatc ctgcctgaaa
    10141 agcatttgga tattaacgga gaagtaatca atccagtagc tcgggattat ccagatgagt
    10201 ttatccaaac aggtatctct gcaatcgatc atttgaatac attagttcgt gggcaaaaat
    10261 taccagtatt ttctggctct ggtcttcctc ataaagaatt agctgctcag atcgcgcgac
    10321 aagcaacggt actcgattca tcagatgatt tcgccgtagt ctttgctgct attggaatca
    10381 cttttgaaga agctgaattt ttcatggaag attttcgtca aacgggcgca atcgaccgtt
    10441 ctgtcatgtt tatgaattta gcaaacgacc ctgcaatcga acgtatcgct actcctcgta
    10501 tggctttgac tgctgcagaa tatcttgctt atgaaaaggg aatgcatgtt ttggttatca
    10561 tgacagacat gaccaactat gcagaggcat tgcgagagat ttctgcagca cggcgagaag
    10621 ttccaggaag aagaggatat cctgggtatc tttacacgaa cttagctact ttattcgaac
    10681 gagcaggaag aatccgcgga ctaaaaggat cggttactca aattccgatt ctaacaatgc
    10741 cagaagacga taaaactcat ccaatccctg acttgactgg ttatattaca gaaggacaga
    10801 tcatcttgac tagagagctt tacaagagtg ggattcagcc accaattgat gtactcccgt
    10861 ctctttcgcg gttaaaagat aagggaactg gacccggaaa gacaagagaa gaccatgcag
    10921 ctacgatgaa tcagcttttt gcagcctatg cacaaggaaa acaggcaaaa gaattagccg
    10981 tagttttagg tgaatctgct ttatctgatg tagacaagat ctatgcgaag tttgctgagc
    11041 ggtttgaaaa agaatatgtc aatcaaggat tctacacgaa ccgttcgatt actgaaacac
    11101 ttgatctagg gtgggaactg ctatcgatgt tgccaagaac cgaattaaaa cgaatcaaag
    11161 acgacatgct ggacaaatat ctgccagaag ggaagtgagt tctgatggca cgattgaatg
    11221 tgaatccgac ccgcatggaa ttgactcgat tgaaaaaaca gttgacaact gccacaagag
    11281 ggcataaatt attaaaagat aagcaagatg aactgatgcg tcaattcatc ctgttgattc
    11341 gaaagaacaa ccagctgcgt caagaaatgg aagagtctat gacagaagca atgtctgatt
    11401 ttgtattggc aaatgcttcg atcacggaac ctttcatcga agaattattt gttttgccgg
    11461 cggaaaacgt tgatttgtct atcgtagaca aaaatatcat gagtgtaaaa gtacctgtga
    11521 tgaattttga atatgatgaa cagctgacag acacaccttt ggaatacggg tatttgaatt
    11581 ccagtgccga actggatcgg tcaatcgatc ggtttactac cctgcttcca aaacttttgg
    11641 aactgacaga aatcgaaaaa acttgtcaac tgatggcgag tgaaatcgaa aagacacgtc
    11701 ggcgagtcaa tgcattagaa tatatgacga tccctcaatt agaagaaacg atctattata
    11761 ttcgtatgaa gctggaagaa aacgagcgcg cagaagtaac aaggttgatc aaagtaaaaa
    11821 atatgggtac cactgactaa gtggtatctt tttttaaaag aggaaaacga tgattaaaca
    11881 aagcaaacaa ataaagaaac gtttgccggc tgttcagctt ctcgcattag gattttttct
    11941 cctgatttta gttggcggcg gcctacttac cctaccattt tttagtaata gtgggcatgg
    12001 gacaaatttt atcgatgcgc ttttcactgc aacttctgct gtctgtgtaa cgggattgac
    12061 aacattgaat acagcagagc attggaacag tgctggtcaa tttttgatca tgcttttgat
    12121 tgagatcggc ggtcttggtt ttatgatggt tccgattcta tgctttgcat tgataaagaa
    12181 gaaagtcagt ctcagcacac gtattatatt aaaagaagcg ctgaatctag aagaagtatc
    12241 aggtgtgatg aagctcatgc tttacatcct aaaattggcc tttgttatcc aattgattgg
    12301 tacggttgct ttatcttttg tatttgtgcc tgaatttggc tggttgaaag gtatctggta
    12361 cagtttgttc catgcaatct ctagtttttg taatgcaggg tttgatttgt taggagacag
    12421 tttagccaat caccagcaaa atgtttattt gatcatggtg gtatctgctt tgatcatttc
    12481 tggtgggtta ggcttcatcg tatggcgtga tttactggaa tatcatcaga aaaagaagat
    12541 gacgattcat tcaaaaatcg cattaaccgt tacaggtacc cttttattag gcggattcgt
    12601 tgtgttcttt ttcacagagc gcaatggggc aacgctagtg gaagggaata tgtttcaacg
    12661 attggcaaac acctttttta tgagtgtgac acctagaacg gctggatatt attcagttga
    12721 ttacctccat atgagccatg caggtctgat tttaacgatg tttttgatgt acatcggcgg
    12781 aacatctggg tcgacggccg gcggattgaa aacaactacg ttaggtgtgt tgctgatcca
    12841 gatgcgttcg atgttgaaag gacgtacgag agcggaagca tttggccgta ccatccgaca
    12901 aggcgcagtg ttgcgggcac ttactttatt ttttgttacg ttgacgttgt gtgtagtcgc
    12961 catcatgatt ttatcggtga ctgaaacgat acctaaaact tctggcatcg aatatattgc
    13021 ttttgaggtc ttctctgcgt ttggtacagt aggtcttacg atgggattga cgccagactt
    13081 gactgcaata gggaaattga tcattatagc gcttatgtac attggacgag ttggtgtatt
    13141 gactgtggtc ttctctctga tgacaaaagc acaaagaaca caatcaaaat acaaatatcc
    13201 agaagaaagt gtcatgattg gataatttca tcttctgaca gaaacctagt gatttagtaa
    13261 aataagaaaa taaaataatt tttcttgctt cttggatact ttaagtgcaa aatgttcgtt
    13321 tattttttga ggcactaccc gttgttgaac ctcgggtagt gcctcttttt tattgaaagg
    13381 aatcaataag atttgattcc tttatttatc aataggttat aattcatatt tttttatttt
    13441 ttcttttacg atacttagtc cagaagctaa aatttcaagg ttattagagg atagggaaat
    13501 tcgaacgaat gcatctggtt gcgcacgtgt agtaaagcgt ctggaatgat aaagtcggat
    13561 accgttagca agaaagtcat tctcaattat cttagggtca atctcaggat caactggaag
    13621 aattcgatag taagggacac gattcgttgg tttttctact ttgaaaagag aatcaaaaag
    13681 ctgattggct ctttttgtaa gacttaattt agtttctaag atcctatttg cttcatttga
    13741 atggaggatt tgagtgatga cttccacatc taatccagaa gttttcacat ttatattaaa
    13801 aattgcttgt tctatcttgc tttggacatg tactggaact acaagataag caactcgcaa
    13861 tccagaacaa ataaattttg tcattccagc taagtagatt gtttggttag gtaataaatt
    13921 ttggaaagga ggaatgatcg tatgttgata gtaagttgtt aaaaatgaat ggatatcatc
    13981 ttcaataacc catagacctt ctttttcaat cacttcggct aattgctctc gtcttttgtg
    14041 agagatttga aaccctatag gattattaca agaaggcata agaaagacac catgaatctt
    14101 cttttttcta cattcctgaa tcaatatatc aggtcgcatt ccttgctcgt caaaatcaat
    14161 tggaacgatc tctaaatgat ataactgagc caattcaatg aaattagcgt atgtatatcg
    14221 gtctgttgct attctatcac ctggagagaa aagtgcagaa agtgtgatag ccaatccatt
    14281 ttgaacaccg gaaacaatag atagagattc taaggacgga atgactcctt gagtttttaa
    14341 ccaatttgct gcagtagtca attgaaaatt tgtgcctttg ggatcttggt aattaagcaa
    14401 accaacaaga tttgggttat tactagttga ttgaataaac gggacaacga atttattaca
    14461 ttcttcaaaa gaactaacca gaccaaaatc aatgatatgt tcggttactt cattggtaga
    14521 gatggtgatg gactcaaaga cattgggaga aataaatgtg ccacttccaa tattcgtata
    14581 cagcaaacct ttctcaattc catatttata agccttgcca acggtagtaa aatttaaatc
    14641 taaataatca gctagctctc tttgggaggg taactgagtg tttttctgta atttattctt
    14701 taaaatatct tgttctaatt gttgaatcaa cgatcgataa taaggacgct tcaatttttg
    14761 tttgtctggt cgccaagtta acgggtaatt gtcataagaa ttaataggca ttttctccac
    14821 tccttaaaat tgtataccat acaattttac cattgattgt attgatttta gaagttaatc
    14881 ttttgtgagg agggaattgt ctatgaaaat tattaatttt gaggaagtag tagcttcttt
    14941 tagttttgaa gaagcgattc aagtaatgaa aaaatgtttt tttgattatg aaagtggtaa
    15001 aatcagccaa aatcctcgat tggtggaaat tttacctgac ggaaaaggca aaaatatgtt
    15061 tgcgatgatg cctgcttatt tgggggagaa cagatatttt ggcacaaaag tcattaccgc
    15121 ttttccggat aatcatcagt atcatttacc ttctcattta ggagagattt tcttgtttga
    15181 tgctcaaaac ggactaccca aagcattgat aaacgcaaat gcagtcacct ggattcgaac
    15241 agctgctgta tctgcgctgg caacagatta cttagcaaat ccgcatgcag aaacacttgc
    15301 tttaattgga gctggtcaac aagcattgtc gcatttgact gcgatttcta ccattcgtcc
    15361 catcactaca gtgtttgttt acgatatttt agaagaaaga cgaaatactt ttatcgctaa
    15421 agctaaaaaa cagtatccta aaataacgtt tataaatgca atatcagtag aagatgcagt
    15481 acgtccagcg gaaatcattt gtacgttgac ctctagtaaa gaggcatttc ttcaaaaaga
    15541 gtgggtaagg gaagacgcac atatcaatgc tgtgggcaca tttactcctg atacaagaga
    15601 gataagtaca tcgttgatgg aaacttctca aatctatgta gatgattact cagctgcctt
    15661 aacagaaagc ggggacctgc ttatagccat ggcagaagga gatttagagg aatcttgtat
    15721 tgttggtaca ttaggcgaat tagtttctga gaaaaaaaca ttttataaag gagaatcgag
    15781 gccgacgatt ttcgacgcag ttggtctagc tattgaagat ctttgttgtg cagaatatat
    15841 ttataacaaa ttagagaaag gggctttaaa atgaacttag attattacgt agttgatgca
    15901 tttgcagatg aggtgttcaa aggaaatcca gcagcggttt atgtgctaga agaatggctg
    15961 ccagaaggta cgatgcaaaa aattgccatt gaaaacaatt tatctgaaac agcatttaca
    16021 gtgaagaaaa accaagaatt tgagcttaga tggtttacgc cagatcgaga aatcgatctt
    16081 tgtggtcacg cgacattagc aacagcattt gttttattta actattacaa aatacctgat
    16141 gaaacgatta aattttctac tcaaagcggt aatctatttg tgacaagaat taaagattat
    16201 tattacatgg attttcctag tattatgccg aaaaaagtgc cgatattgtc tgaatatgaa
    16261 gaagcgattg gggctaagat taaagaagcc tacttagcac gtgatttatt tttcgtatta
    16321 gaagatgaaa aaacggtggc taaattgcag ccagatttta cagctatcaa aaattttgaa
    16381 ttaggaatag gagtcatcgt gacagcagaa agtgtgcaaa aagactttgt ttctagaacc
    16441 ttctttccaa aattaacaat caatgaagat ccagtatgtg gatcagcaca ttctaatctt
    16501 attccttatt gggcagaaag aatgaataaa acaaaattgt cagcttatca aatgtcatca
    16561 aggggcgggg atctagaatg tgaattaaaa gaagatcgtg tgataatagg aggaaaagca
    16621 gttttgtttt ctaaaggtac ctcgtttatt tgatttcttt caaatgaatt aaaaataaat
    16681 tggtggagag tggcaacctt atagctacaa tggttgttaa aaaaagacgc aaactttttc
    16741 aatttaatga aaagtttgcg tctcatttta attattggtg tttgtttttt attcaatcaa
    16801 gagtaaaatg ccccatacta actatcctcg gatgacttcg attttataac catcagggtc
    16861 aatgacaaag tagtaagaag gcgcagtgcc aggtaatcct tttaagtcag taatctcaag
    16921 accggcagct ttatgttgct cgtgtaattt ttctagatca tcagctgcta tagcgatatg
    16981 accatagccg tctcctagat catacgcacc atgatcatag ttgtaagtca actctaattc
    17041 atagtcatcc ccaggaagtg tcaaataaac aagtgtaaat ttgttttctg gaaaatcacg
    17101 gcggcggctt tcttcaaaac caaatgcctt ttgataaaat tcgattgatg cttcaagatc
    17161 tttcactcga acacatgtgt gagccatttt catagattca tctctccttt tccttttact
    17221 caatcataac aaaaagaagt aaggaaatag ttgatttgtg cttgttcatg agaaatttct
    17281 catgtaaaat ggatagaaaa taaaactgtt aaggtggaaa ctattcatgg ataaaccaat
    17341 ttttggtgaa aaaaagcaat ttgctgatta tcaaacccgt tatgcagcat atattgtcat
    17401 agaaagagat cagcagattg cagtaatcga ggctccaaat ggtgcttttt ttcttcctgg
    17461 cggggaaatt gaaggagaag agacgaaaga agaagcgatt gtccgtgagc tgatagaaga
    17521 aatgggaatt gtggcagaaa tttcttctta tctgggagaa gctgatgagt atttttactc
    17581 gaattatcgt gcaacttatt attataatcc aggatatttc tttgttgctg attcttggaa
    17641 gcgtatagga gaaccaactg aaaagaccaa taaaatttgg tgggtgagcc ctcaagaagc
    17701 gcttgtgaaa ttgaaacgtg gtagccaccg atgggcagtc gagagatgga tcgaaaaaaa
    17761 taaaaactga aagaaagcga gtagatcagc tttctctcag tttttttatt tattacagta
    17821 catatttttt gattgcttca gctacaccgt gctcgtcgtt tgatgaagtg atgacatttg
    17881 ctgcttcttt gacaagagaa acggcatttc ccattgctac tcctagacca gcatattcaa
    17941 tcatggataa gtcgttctcg ttatctccga tagccatgat ttcatcttga tcaataccaa
    18001 ggtgctgtgc gagattcgca actgccgcac ctttacttgc ttctttattt agtacttcga
    18061 agtaataggg cgcacttttg accgttgtgt atttatcacg gaaagtttgc ggtaaacgcg
    18121 caattgctgc atctaaaatt tctggttcgt cgatcatcat cattttgacg atgttcatgt
    18181 ctggtgtcat ttcttcaggt gttcgatatt ttagcggcat cttgactaaa cttgcttcgt
    18241 ggatcgtata agcgctgata tctcgattag gcgtgtagat cgctttgtca tcgatcgaat
    18301 ggagatgact gccgacacgg cgtgccatca cttcgatctc taggaaatct tcatgtgtca
    18361 gtccgtgacg ggcgatgatt ttacctgatt tcgtttgttg tacgagtgcc ccgttataag
    18421 taataacgta atcattatct tgaaaaaggt tcaactcgtt taactgttcc tggacacctg
    18481 gtaagggacg gccagtgcat aaaacgatcg aaacaccttt tgcaacagca gcggcaatcg
    18541 cttcttttac ttcttttgtt acttctcttt tctcatttaa taaagtacca tcgatgtcga
    18601 tagcgactag tttgatagac ataatttcct ccaatatatt ttaatttatt tcaatcaatg
    18661 cgccattacg gatatggctg gcaaattctt gataagtttc gtcaaataaa tcataatgat
    18721 cacgcaagga cggatcaacc atttcttttg gaaagaaaaa tcgctcatct ccttgttctt
    18781 gtcccgctaa tgcagcaaca agctgactaa cctgtgataa ttctatcaaa gttccgtcat
    18841 ttcgcaaaat ctcgatttgt gtgcgatgtc tgccttgttc tgggcgataa aaatcataag
    18901 gtagatcgta gctagaattc acagcagtgt aataaacagg attatatccg accttttcta
    18961 ctaacaaagt gagttcttgg actaattttg aatcccggtt gccagaaaaa gtagcagatt
    19021 tcagtggttt acgattcaaa aaacgtttag ctaaatcatt taaaatagga tcagcagatt
    19081 cagaccattg agtaaagtaa gtgcccaaga ccccatcatc aagtttgagg tattcttcta
    19141 aagtaaagct tccgtcaaga aatggcagaa gtaattgaga atggagttca aaactgctgg
    19201 catcttcttg atacaactcg tgtgcgcgat gaagcagatg ctccaaaatt acttccatac
    19261 cacgggaaac aggatggaag tacacttgca catacatttg atatcggcta acgatataat
    19321 cttccactgc atgcatgcca ctcatggaaa aagcgatccc gccttcataa ggacgaatca
    19381 cacgtaatat tcgggtaaga tcaaatgttc catattctgt tccagtgaaa taagcatccc
    19441 gaagcaaata gtccattcga tctgcatcga tctgactaga aatcatttgg acaacttgtg
    19501 ggttggggta agttttttgg atcacactag ctaccttttc aggaaaacct tcttctactc
    19561 ggttaagaat ctgatacact tctgtttctg gagaagtgat gatctctaca gtgatggctt
    19621 catgattcgt atggaagata tgttcaaatg tatgagagta aggaccatga ccgacatcat
    19681 gcaacagtgc cgcacataaa gtcaccagcc gttcctgatc atcccagcct ccgttgccta
    19741 ttttttcttt tgaaaaattt cgagaaaaga tatcgcagat tcgtcttgaa atctcataaa
    19801 cgcctaatga atgagtaaaa cggctatgtt ctgctccgtg aaaagtgaaa gaagaagtgc
    19861 ctaattgttt aatgcgtcgt agacgttgga cttctttcga attgatcaaa tctaagatta
    19921 cttgatgctg gacatggata tagttatgga ctggatcacg aaaaactttt tccataggca
    19981 gcatttgata tttataaggg gttgtcataa tgtagctcct ttatgttatt acgttgttcc
    20041 attctctgga tttggttgtt ccattgctcc agctggattt ctttttttag ccagtcggta
    20101 gaatcctgta aatcacttgc agaatacagc ttttcaaaag cttttgcaaa tgcttctttg
    20161 acttctttta caaaaagggg ttggtcaagc agttcttcaa gtgttgccat tgtagcagga
    20221 tccactgcag gatagccatt tgtaccaaag tcttctttta aagcagattg ataaaatcgc
    20281 ttcatgactt gcccgcgttt ttcctgatta ccgttgacac ttaagtacat cataacagaa
    20341 atcccatttt tgatccgacg ctgagcgata ccggcaaatt ttttgccatg gatgcttaaa
    20401 tcataagttc caggacaata agaatgggtg atttcgaatg cttgtatcgg tgcctttgga
    20461 aaagcgtatt tagtcaatag gagcatttgc tcgtaagcgt catctatggt tatttttttt
    20521 gtgtcacttt gggggaaaat caaagaaata ttcaaaattc cttcatctga tacgatccct
    20581 aatccacctg aatttctgat cactggagcg taacctgcat ctttcagtgt ttggatcccg
    20641 tcacttagaa aaggagttcg aacatccttc atacctaaga tgactgtgca gtcatattgc
    20701 cagaaatgta aaaagatttg ctggtgttct ttagaatatt ccgttaatat atctgttaat
    20761 gcaaaagggg aaaagtcgtt tttttgataa atgtgctgat ctagtcgaat cgcagggttc
    20821 attcaaccgg ctcctttgta taaattcata ttttctacaa tattatactc taaaatcaaa
    20881 gagttgtttt ggaaaaaagg ttttctgtaa aaatggacaa gttggttgac aaaacaaaag
    20941 aaactaggaa caatgtagct atgagaagta aaggagaatg aataatggaa ttatcacaag
    21001 gaacaccgat ttcaatcaaa ttgcgtacaa aagtaaaaca gcaaggggaa gtgcaagatt
    21061 tttattttga tttgaaaggg caaatggtga caattggcga tacgctgtac atacgttata
    21121 aagagattca ggaggagacg ggcgatgaga ttcctgtaac gatcaaatta gttccagatg
    21181 gtcatgtaca gctgatccgt gcaggagaaa tgcggatgcg tttgaaattt ggctataaag
    21241 aacggttaga aacatcctat cgaacaccat acggtatgat ccagattgct acttttacga
    21301 aagaactgca tgttagtttg aaagaccgtc ccacaggagg gaaggttcga atcgattatg
    21361 atctgttcat gggaccggaa aaaatcggcg aatattattt gaccttggat tttacagcgt
    21421 aaatataaaa gaaaagtttg attttttgtg tctgattttg tatgataagg agtagactgt
    21481 gaaaggacgt gtcctgattg gaaattagtg tatttgaagg tgcaaacaaa agcgaattat
    21541 caatgatcga agtagcacac gcgatcttag aacaacgtgg cgatgtcatg gatttttctg
    21601 atttagctaa ccagatccaa aactatcttg ggaaatcaga cagtgaaatt cgtgattcac
    21661 tcgcacaatt ttatactgac ttgaatattg acggcagctt tatctcatta ggggataatc
    21721 gttggggctt gcgttcatgg tatgctatcg attcgatcga tgaagaagta aaccatggaa
    21781 tggacgaaga ggatgaagat actccacgtc gccgtaaacg caagaaagtc aacgccttta
    21841 tcaatgatga tgaagacgcg atcgattaca atgacgatga cccagaagat acagatctga
    21901 cagaagaaga tgacgatgat ctgtttgatg acgatgatga tgaagatgaa gaaatcgctg
    21961 cttataattc tgacttgcaa gaaatcggag cagatgaccc gacagatgat gaagaagact
    22021 tgccaggaaa tatcgaagaa gatttaagta tcatagacga agacgatgat gatgaagatt
    22081 atgaagagga agaattttct gactctgaaa aagaataaac attattttct tacgagaggt
    22141 tgtgacagag acgatcagcc tatcagaata aggaggtgtg acacgat
//
LOCUS       DARBDV010000043        22031 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000043 DARBDV010000000
VERSION     DARBDV010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.043"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            367..555
                     /locus_tag="SCM56_001542"
     CDS             367..555
                     /locus_tag="SCM56_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110323.1"
                     /translation="MELIVFTNNGQTYHFYEVENFKPTTTGFSFIYTGKATGVTRSAT
                     FNNTSTAGYALAEVQNES"
     gene            545..805
                     /locus_tag="SCM56_001543"
     CDS             545..805
                     /locus_tag="SCM56_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110324.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            1138..1710
                     /locus_tag="SCM56_001544"
     CDS             1138..1710
                     /locus_tag="SCM56_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110325.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            1711..2022
                     /locus_tag="SCM56_001545"
     CDS             1711..2022
                     /locus_tag="SCM56_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEG1110326.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            2013..2273
                     /locus_tag="SCM56_001546"
     CDS             2013..2273
                     /locus_tag="SCM56_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110327.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            2434..2793
                     /locus_tag="SCM56_001547"
     CDS             2434..2793
                     /locus_tag="SCM56_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110328.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            2790..4433
                     /locus_tag="SCM56_001548"
     CDS             2790..4433
                     /locus_tag="SCM56_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEG1110329.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            4468..5550
                     /locus_tag="SCM56_001549"
     CDS             4468..5550
                     /locus_tag="SCM56_001549"
                     /inference="COORDINATES: protein motif:HMM:NF028182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110330.1"
                     /translation="MKKRYFLFVTIFIIIAICVLTVFLPQITEYLNYRIFTTKVDDSV
                     TSLSRLEFIKICASVFGPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKEL
                     LTQIDREFYFLLNMFIEIQKDQNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEI
                     GSTIEQSGPLKGTVEYIKFETFDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQ
                     IYLGRYFKMFHRIVKTLNEYYDDYNDFDVKRYTKYIGTLRTQISPAEFQVILFNSLYI
                     KRGFGLGIQLIGSGFFGDDFDFETNQHFETSINEQWFLSLSTVDSDNSIKRQKLSEYI
                     KELGSVEYKKIANFESLYKLFNETKL"
     gene            5594..6766
                     /locus_tag="SCM56_001550"
     CDS             5594..6766
                     /locus_tag="SCM56_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEG1110331.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            6753..7457
                     /locus_tag="SCM56_001551"
     CDS             6753..7457
                     /locus_tag="SCM56_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEG1110332.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            7462..8622
                     /locus_tag="SCM56_001552"
     CDS             7462..8622
                     /locus_tag="SCM56_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEG1110333.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            8698..8976
                     /locus_tag="SCM56_001553"
     CDS             8698..8976
                     /locus_tag="SCM56_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEG1110334.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            8957..9328
                     /locus_tag="SCM56_001554"
     CDS             8957..9328
                     /locus_tag="SCM56_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HEG1110335.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            9344..9748
                     /locus_tag="SCM56_001555"
     CDS             9344..9748
                     /locus_tag="SCM56_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110336.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            9745..10155
                     /locus_tag="SCM56_001556"
     CDS             9745..10155
                     /locus_tag="SCM56_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110337.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            10216..10803
                     /locus_tag="SCM56_001557"
     CDS             10216..10803
                     /locus_tag="SCM56_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEG1110338.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            10876..11343
                     /locus_tag="SCM56_001558"
     CDS             10876..11343
                     /locus_tag="SCM56_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HEG1110339.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            11389..11697
                     /locus_tag="SCM56_001559"
     CDS             11389..11697
                     /locus_tag="SCM56_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110340.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            11892..18626
                     /locus_tag="SCM56_001560"
     CDS             11892..18626
                     /locus_tag="SCM56_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEG1110341.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            18630..19502
                     /locus_tag="SCM56_001561"
     CDS             18630..19502
                     /locus_tag="SCM56_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HEG1110342.1"
                     /translation="MRTVILRNKLNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSF
                     REIGESIELSEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSL
                     KSLSKTEIDSQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYT
                     KNVWEKKGVFKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFI
                     NMKDTQILEVSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGH
                     ANVEIELYEERDLF"
     gene            19499..20548
                     /locus_tag="SCM56_001562"
     CDS             19499..20548
                     /locus_tag="SCM56_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110343.1"
                     /translation="MILAITLFHRDMNLYNERLISTEFEFGTDEIGEEVSNFITDKYV
                     HVKSGDFLLAKFIPSGKIAYFGVISSQEDKKINCKGLLSLADSEIPTVKISGANYEEH
                     IRRLIEYYLLNDPTKQLKNILEVKTESVTSHSYQAVDTNKRRLNAYIINGFKKYNVKW
                     YVKEIKGKKIYTGIRAVDDSIYIKDNSSEFNDWDVFVQSPGPGNENKLLIVDKAMKDI
                     ENPLVLSTWYLDDENNLTQDSTNKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGN
                     AYSHEIKVNVVLNARNINVENIETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFG
                     NVRSRFMDYFEDNGG"
     gene            20552..21376
                     /locus_tag="SCM56_001563"
     CDS             20552..21376
                     /locus_tag="SCM56_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110344.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            21394..>22031
                     /locus_tag="SCM56_001564"
     CDS             21394..>22031
                     /locus_tag="SCM56_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HEG1110345.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKANNRIDQTNQQIGDLGKLKKMYSNSI"
BASE COUNT         7878 a         3297 c         4671 g         6185 t
ORIGIN      
        1 cttctaaatt gttcttatta tctatcatca gttgctgtct attgtcatta tgtcactgtg
       61 gtggaaaggt gtatcgctca tctaaaattt aggtgcaaac tgcaaggttc gattccttgc
      121 cagtgacttt ggtaaccgag gcatcggcgg tttaaaaata taggggtgcg caatttcgta
      181 cgcgttttgt gcatcttgta ggttgctatt acataattgg ttgggttaga ttgagttttg
      241 ggattcggta caaatgaatc gtcaaatgac tcaagcacag gatcggaaac gtccctgcct
      301 gtgcattaca tattagatca ctctttgagt ggtcttttta ttttaaaaaa gagaggattt
      361 tgaaaaatgg aattgattgt atttactaat aacggacaaa catatcattt ttatgaagtg
      421 gagaatttta agccaacaac aacaggattt agttttattt atactgggaa ggctacggga
      481 gttactcgtt cagctacgtt taacaataca tcaacagctg gttatgcgct tgcggaggta
      541 caaaatgaat catgagaagt ttatcgaaaa atgcaaggct attgtacgtg aaagaattga
      601 aaatgagatt gctgacctaa gtggagcagt gcctgagttt agtattttca tagtctggtc
      661 atgcaaaaca ctgcaaaata gcaaagcatt agtcagcgct agcttaaaag gagcaccgta
      721 ttttgaaatt acgttgaacg gggacaaagg tgaaatctat gtagatactt atctcaaaaa
      781 atcaaatgaa tgtatcaaag tctagcaggt gctaggcttt ttctttacat aaaggaggct
      841 gcataatgag aaactactgg tatgtatcac taactaatga atatcctcga accattggtg
      901 attgttcagt gcgtgttgtg cgttctgtac aaatcaaagg gaagtactct attgtcgaaa
      961 tgctaagaga agctacacca aacgaagtgg ataaatgcaa gctgatatat tgcggtcatg
     1021 gctattggaa agacgagtat atccaataca acattgagag gtacatcaaa tgaatgataa
     1081 ctatgattac atcaagttgt tagcaagtaa ttattagatg tttttaggag gaaaactatg
     1141 aaattaaaag attatatcag agaagggtat aacgttgtaa ctgcacccaa tctagcttac
     1201 aaaattcaag aagattatcc caatgcttta gtgtttactg atagagcttt agatgccatt
     1261 cctataggga agttattagt agatcgtttt tatagcaata atccagctgt tcttagtgct
     1321 aagccacttc aaaacgctta tgctattgaa cgctttactt gcgagtttac tggatatgag
     1381 acggtagtac caatgacagg tgagacagga agaaaagtaa ttcaacagat caaaaagagg
     1441 attgctgaat tgacgttaga tgatggaagt aatacgaacc ttctagaaat caaactaaga
     1501 gatactgact cagtaccaga agtttattat aaaggtcaac gaatggatga gatgccgata
     1561 ggtcttgtcg atattactta tcattgggat acggaagaat atgaatgcca agtaccaaaa
     1621 ggagctaacg acatcactat tgaatattat gattcacata ataacaagta cttagataga
     1681 aaaatcattg ggcacaagag agatatgtaa atgattgatg tttcaagtaa acaagcaaga
     1741 gctaggttct atggatcaac tgagtggagg aaacttagaa gtcaatgctt agagcgagat
     1801 cattatgagt gccaatggtg taaagcagag ggtagactaa caactcaaca tgattcaatt
     1861 cttgaagtgg atcatataaa ggagctggag tatcatccgg atttagctct agatataaac
     1921 aacctaagaa ccttatgcaa agattgccat aacaaaaggc acaaccgtat gaaatatcgt
     1981 ggccaacaaa agaaaagaaa gtgggatgat gaatggtggt aagaaaaaca gcaaacagag
     2041 atgatgtcaa acgagcagaa gcaatcgcta tggttgataa ggctattgat aagttggttc
     2101 tatttaagag tcagctcatt gaaggaacaa caactgtaga ggatatatct atcaacaata
     2161 gactaatagg taacgctaat gaagtaacag ggacatttaa tattaatgta gacattgatt
     2221 ataccttgag tccatttgca catattgtag aaagcaacga gcaagacggc tgataaatca
     2281 gtataaagcg aagatatata gagtgaaata cccccggtca aaagattttg agaagaaata
     2341 aaatttttgg gaaccggtgg atggggtcaa ctctgcaaat aaatgatgct attttcacac
     2401 gaccccctcc ccacgtagaa aggaagtgag ttgatggctg atctgcaaga gagaaatagg
     2461 cttgtttctg ctgaagaaag gcgtctaaat aaattgttta aggatattga taaggataaa
     2521 aagaaggtcg tttctggttt gattacgcaa gcggcacgct taaaaattct tcttgatgaa
     2581 atgtggattg atatttccga aaaaggtgat tatgagcttt tctctcagtc agaaaatcaa
     2641 aaaccttacg agcgggaacg accagttgct aaacagtata attcgagaga ccaagcatat
     2701 caacgtgtaa ttaagcaact tactgactat ttgccagaag aaaagcgcga acctacaaaa
     2761 aatgcagcgc tagatggtag tgatttgctg tgacaacatt aaagccattc tattttgatg
     2821 aatacgtaga tttatacgaa aaaggggtaa tcccttttaa taaagaaaga attcagttaa
     2881 ttacttattt aaaaaacgaa gtgctagttc gggatgatat ctattttgat gaagaaatga
     2941 ttcaaaagtt tatcaagtac actgaaaaaa atttttttcc tttagctaaa tatcaaaaat
     3001 ttatcacacc tttcattttc tgctatcaaa aagaggacga cgaggtgttt tttgatgaaa
     3061 ttctaaatag tattgcgcga ggcggcggga aaaatgggtt catgtcagca agagactcat
     3121 tttttattag ccctctctac ggtgtcagaa attatgatgt aacgattaca gcgaattctg
     3181 agaagcaggg aaaggtatct tttaaagaag tttatgaaat ggtacaaatg cgcaaactgg
     3241 aaaaacaatt ttatttgact aagatggcta tagtcaacaa tgtaactaat tcgattttta
     3301 gttataggac aaacaatcct aagactatgg atagtgcaag agatggctgc ttagaatttg
     3361 atgaaatcca tcagtttgaa tcttcagacc tagttgatat tcagcgtagt gggctaggga
     3421 aaataaaaca tcctcgaact ttttataatg gtacaaatgg tcatgttaga gaaggttttt
     3481 atgacaaaat gttggaacga gcacaaaaga tttttagtgg agagaacaag aatgatcgct
     3541 tgtttccctt tatttgtaaa ctggataaca ttgatgaaat ggataatcct aaaatgtggt
     3601 caaaggctaa tccaatgttc gaagaagatt cagcatattc aaaacgtctg ttttctacag
     3661 tgatgaaaga atataaaaaa ctagaagatg agccatcagg taggcgtgaa tttgttgtta
     3721 aacgaatgaa ttttacagaa ggtgatgcag aatcagatat tacgactcat gaaaaattaa
     3781 tggctactaa acaagaattg ccaaatttga atggtcgttc ttgtgtggct ggatttgatt
     3841 atgcaagtat tcgtgatttt gcaacagtgg gtttgctttt taaaattgat gataaattta
     3901 tctggcagca acatagtttt gctaggaaaa agtttctaga tacattcaaa ctgaaagcgc
     3961 ctattaagga ttgggagaaa aaaggtttag tgacaatcgt tgatgagcca tcaattgatc
     4021 ctcgtcattt aattgattgg ttaaatgaaa aaagaaaaat ccatcaaata gaaatcgttt
     4081 gtgccgacgg atttcgtatg gatttgctga aacctcttct agagaaagaa tcatataatt
     4141 ttgagtttat cagaaatata agaggtgtgc agtcaaaagt tgctcctatt attgaagatg
     4201 gttttgcaaa tgaacggttt atttttggtg atgatcctat gatgcgttgg tatacaaata
     4261 attcgtttat caaagaagac aaattaggga ataaaacatt tttgaaaaaa gagccagtaa
     4321 ggcgtaaaac agatgggttt catgcttttt tggcagcttt atataaacga gaagaaataa
     4381 ctgatatgga ttatgaggaa gcatttgata tgttggatga gattgaattt taaggtatac
     4441 tccttattga aaggggtagt tatagaagtg aaaaagagat attttctatt tgttacaata
     4501 tttataataa tagcaatttg tgttttgaca gtatttttac cacaaattac tgagtattta
     4561 aattatcgga tatttaccac aaaagtagat gatagcgtta ctagtttaag taggctagaa
     4621 tttataaaaa tatgtgcatc tgtgtttggt cctattttga ctgtaatggt atttgtgaat
     4681 actttaaata tgcagaaaaa aacagagcaa aagcaaaatg agcttgaagt aaagcaagct
     4741 aaggctgaga acgagagagc aaaaaaagag ctgttaacac agatagatcg tgagttctat
     4801 tttttattaa atatgtttat tgaaatacaa aaagatcaaa atgccaaaca agctgttatt
     4861 gctttacaga agttggcaat ttttaacgaa aagggtttta atcaagacta tgcatttact
     4921 tctaaagatt tatcagatag tgaaattggg agtacaattg aacaatcagg accgctgaag
     4981 ggcacagttg aatatataaa atttgaaaca tttgatgtaa gcaagtcgat ggggaattat
     5041 caaacaaaaa caaaagacgt tgctacaagt ttacacgatc agaaagtagc tatattgaat
     5101 gaacaatatg aaaagatgca aatatatctt ggacggtact tcaaaatgtt tcaccgaata
     5161 gttaaaacat taaatgagta ttatgacgat tataatgatt ttgatgtgaa gaggtataca
     5221 aaatatatag ggacattaag aacacaaatt tctccagctg aattccaagt gattttattc
     5281 aattctctat atataaaacg aggttttggc ttaggaattc aacttattgg aagtggtttt
     5341 tttggagatg attttgattt tgaaactaat caacattttg aaacaagtat caatgaacag
     5401 tggtttctat cactatcaac tgttgacagt gataactcta taaagcgaca aaaattatct
     5461 gaatatatta aagaattagg atccgtagaa tataaaaaaa tagctaattt tgaaagttta
     5521 tataaactct ttaatgaaac taagctctag aaatagggct tttttatttt tctttgaaag
     5581 gtggtgaaaa catgtgagct tatttgatat ctttaagcag tcaataagaa atgaagagcc
     5641 ttctgagtgg ataccggatt ttgtatatgg agaggaagta tctgctcggg catacctcaa
     5701 aattatggca aaaaatactg ttttagattt tgttgcaaga actatgtcaa cgttagaagt
     5761 taagtttaaa aataaagatg ggacatcaga ctgggattat attctaaatg ttcgacctaa
     5821 ttctgatatg tcagcagcta cattttggca aaaatttttt tatcggctct tagatgacaa
     5881 cgaagtgtta gtaatattta cagatgataa tcaattgctg attgctgatg attttagtcg
     5941 aacggagtat gccgtgtatg atgatgtttt tacaaatgtg atggtaaaaa attttgtctt
     6001 tgaacgttca tggaacatgt ctgacgtcat ttatatggaa tataataacg aagaattaga
     6061 aaggtttaca cgaggtttat ttgaagatta cgccgcgttg ttcggacgga ttctagaagt
     6121 tgcaatgcgt aacaaccaaa ttcgtgggtc tgtatccatt gattcaacgg gaacggcgaa
     6181 tgatgaaaaa ggaaaagatg gtaagactag atctcaaaga ttacaagaat atattgatag
     6241 gatttggaac tcttttaaaa ctaactcagt tgcaattgtt cccaaagtga aaggctttga
     6301 gtatgaggaa tacaccaata aacaaggcgt atcgaatcaa tcgctagatg aactgaataa
     6361 gatgaagtcg tctttaattg atgatgttgc caacgccata ggagtaccta cggcgcttat
     6421 ttatggtgaa aaagctgaac tggattcaaa tattaaagct tttcgtaaat tgtgtattgc
     6481 tccactagtt aaaaaacttc aagacgaatt ggtggcaaaa ataattagtc gtcaagagta
     6541 caaaaacggg gaacgtatag aagttaaaaa agttctccaa aaagatctat tagagtatgc
     6601 tactcaaatt gataaaataa catctggtgg tgcgttttat attgacgaag ttcgtgatga
     6661 agttgattat gatgagttgc caaataatga aggaaaaatt cgtattcgta cgaaaaacta
     6721 tgaagaaact gtgaagggag gtgagaacga caatgccgaa agctaaaaaa gtaccatttc
     6781 agtttactaa cgaaattcag aatggtaagc acattctcac cttgagtgga aatgtacaga
     6841 aaaaatattg gcgtgatgat gatgtcataa acgctaaaga tattcgtgac tctctagata
     6901 ctgtaaccga tgatattgta atcaagttga acagcccagg cggtgatgtg tttgaaggga
     6961 ttgagattta caattattta aaagatcacc catcaaatat tactgtagaa gttacagggc
     7021 ttgccgcaag cgcagcaacg tttattgtcg ctggtgccaa tgatgtgatt atgaatgttg
     7081 ggacttcttt gatgattcat gaagcttcta catttgcttg ggggaataaa caggacatcc
     7141 aaaaaacgtt aaacgcattg gaaacgatcg atgattctat cttagctatt tattcagata
     7201 agactggcca atctgttgat aagttgcgtg aatggatgaa tgaagaaaaa tggtttacag
     7261 cagatgaagc tgtcaaatat ggatttgcca attctgtgaa gcgtgaagaa ccacaaaatg
     7321 aacctcaaaa tattgcagcg atgattcaag atgctgttgc cgttgcaata gcgaatttaa
     7381 aggaacctgt tactaataaa atcgaacaag aatcaaaaca aaaatcatta atcgcacgat
     7441 tgcgaaaagg agaataaaga tatgttaaaa attacagata aaactgcaga tgcaaaaaag
     7501 gcatttaatg ctatttcttc gaaagaagat gcaacacccg aacaaattaa tgctgctcta
     7561 gaagaatatg taaccgccat tgcagaagat gccggcaaac aggtgcgtgc tgaatatgaa
     7621 gaattgaaaa atgtgacgga caaccgtgta ttagaggctc gtggaattcc tacactaaca
     7681 gcagaagaaa caaagtttta taatgaagcc gtaaaatctg gaggtttcga ttctgacgta
     7741 gtttggccag aaacgatttt ggaacgtgtt tttgaaaatc ttcaaaacga tcatcctatt
     7801 ttaggcctca ttaactttac accaactgta ggtcgtgtca aagtaattag agcacgtcgt
     7861 aaaggtgttg ctgtttttgg accattgcat aaagaccttg aaggccaact agatgctgaa
     7921 tttggtgcaa ctgaatttgt tcaacttgcg ttgaccgcat ttttcttaat ttctaatgat
     7981 accttagatt taggaccacg atggattgac cgttttatca atctttcttt aagtgaagca
     8041 gtacgtgatg tttgggctaa aaaaattatt acaggttctg gtaaagatga gccaattgga
     8101 ttgcttaaag atctagatgg tgcggtcaca agtggtgtat atccagataa agctagcgca
     8161 gggacattaa cttttgcgaa agagaagatt gttcttgaac ttgcaggggt gatgaaaaaa
     8221 ttatctaaat acacatacaa agtagatggt gacgatgaag gtgaagtgaa ataccgttct
     8281 gttgctggac gagtgtatct aattgttaat cctgttgatt actgggatat cgtagctaga
     8341 gttacatttg caaatttaaa caatgtgtat gggactacga tgccatttat cgctgttgat
     8401 catatcatcg aatctgtaga cgtaccagaa agtaaatgta ttgcatacgt tgataaagag
     8461 tatgaagcta ctcaatcaag agctgaaaaa gtctatgtat ataaagaaac ttttgcaatg
     8521 aaacgcgcaa ctttgtatgc tgtcgacatg cttggtaatg gctatccaac aaataatgat
     8581 gcagcacaag tatatgactt ggactttaca gcgggggagt aatgtcccct gcaattggtg
     8641 agataacacc gacgactgat ggggcagtca ttaatctttc gtaaatgggg ttagagtatg
     8701 aacactgaag aatttataaa agaatataag gcccgatttc gtattttcca tacttccgaa
     8761 gatgaggata tcgggaaaca gttagagagc gggtttgccg atataaaatc tctaatcggc
     8821 agttttgatc cttccactta tgagaaagga aaagaattag tttatgaacg tactcggtat
     8881 ctacggaacg aatcactaga gtacttttac gacaattttc aaacaatgat tatggatgca
     8941 tcaattgatc tggcaggtgc tggatatgcc aattaaatca aattataaga agcctaaaat
     9001 tgtagctggt gatctaaata cgccagttac tttttttgag tttcaaccta ataagggtcc
     9061 agaacctggt gatgaagaaa aaaaggaact gtatttttgt acagcactag tttacaaccc
     9121 atcaatgaaa gatcgtgaca ttcttggcac aaaagaaggc gttacgataa aaatacggga
     9181 tccccatcaa tcttatattc ctactaacaa acataaagta aggatattag attatcgata
     9241 tggagataaa gtttgggaaa taatcgatgt ctcaccggat ttggaagaaa attcaattgt
     9301 aaagatagta ttagaattaa ttacttaaat ttggtgataa atgatgagta acgtaaagtt
     9361 tgaaggcgtt caagaaacaa tccaagcttt agaaaaaaag tatggagaaa agaaaactaa
     9421 gacattaaca aaaaaagcaa tcaatgttgg cgctgaaaaa gtagaaaaac agttgcaaac
     9481 ggacatgacc gtttttaaag acaaaggtta tacgatcgct gaggttgttc gcaagaacgc
     9541 tacgtataga gattacaagg cagaagctga aatcggttgg aatggtccac atcaacgata
     9601 tagaatcatt catttgaatg aatggggata tacacgaaag ggcaaacaaa ttagaccgag
     9661 aggatttggt gtaataacaa aatctcttaa gaattccgag ctgttgtact ttgatgctgt
     9721 aggagaggag gttaaaaaga gtttatgaaa gatatgctta atatcattta cgaggctctt
     9781 tgtttgaatg agtatattca ttcaatgacc tacaacgaga ataccgagca atatcgtatt
     9841 aaattttatg agcaacctga aaccgccgat aaaacaggtc caatgatcac aattcgtcct
     9901 gtagatacac ctaacgaagc gtaccacgga agtaacaaag agctttctgt ggagcatatg
     9961 atacagattg acgttgaatc gaaatataga gcaacatgca agcagattca atatgagata
    10021 aaaaaagaga tgaaaaaact aggctttggc caaatttccg gtcaagggtt agatgaatac
    10081 tttactgaaa caaaccgctt tgtagacgcg cgtcgttacg aaggaaatac aaaaatttac
    10141 gataccgaat attagaaatt aagacacgaa aataacgtgt ctttttttat tacccaaaat
    10201 taggaggaaa attttatgac tttagtagga ttcaaaaaaa tgacaatcgg ggtctttgac
    10261 agcacaggga agattccagc agcaaattta tatgtaattg aaggtgaaca aaacaaaggg
    10321 gcgactgtat ctgctgaaat cagtgggcta tcaaaagaac catcaaaagt ttacggatcg
    10381 aacatcgctt actatgtctc tcaaaaagga acaggcgatg tttcagctac ttttggattg
    10441 ttagatttac caacagaagt aaatgacaaa attcttggtt acaaaaccga tacaaacaaa
    10501 atcagcttct tgggagaaga tactgagcca ccatattgtg caattttgat ggaatcggag
    10561 gatttgaacg gcgatacagc aatgcttgcg atgttcaaag gaaaattcag tcgtgaatct
    10621 attaatctta acactacgac aaatgaagct ttcgaaccag aagcagaaga atatgtattt
    10681 tcagcgattg ccaatgatgt tgaaggtgat gctaaaggcc aaacagtcgc aaaatatgtt
    10741 ggagacgaag aagcttctat tactggtttg aaagagatgg tatttcctgc agcgggggag
    10801 taactagccc cgttgttgga gcaatcactc ccactattaa tggggcaaca gttgaattaa
    10861 gttaggagga taactatgcc agatacattt aaaatctata aaaaaaatgg tactgaattt
    10921 acaaaagtcg cagagggtga aagtccactt tctattactg gaattgcagc aaatactcaa
    10981 gttgctaaag gagattatca agctacgcgt gtggtcggag gtcaagaatc agtaaaagta
    11041 gatattcctg cattcaccac tcttccaatt tcagttactg gtgtgacgtt ggataaaacg
    11101 acagctgaag ttgaacaagg cggaacctta aaattaactc ctactgtaac accagcaaat
    11161 gcaacagaca aaacaggaag ctggagcagt tcgaatacag ctgtagctac ggttactgga
    11221 ggcaacgtta cagtaaaaac agacgctgaa gttggcggaa ctacagaagt ttcatttact
    11281 actacggatg gcggtaaagt agcaaaatgt actattacag ttattgaaaa agcagcgggc
    11341 tagtcttcgg actagctttt ttatttggaa aataatggag gaatctaaat ggcaaaagta
    11401 agaattgaat tgaaagaaga atcaggaaag aaagtctatg aaaacataga cacaacaggg
    11461 aaagattatc gaaaagcttt agaaacaatc aaaaagctaa atgaagacgg agtaatgatt
    11521 tggaatcgtt tagacattta tttggatttt gctgtaggta tcttcaggga tagcaaattg
    11581 acagctgaac aaattcttga tggcttacca tctgagaagg taatgagcac attggacgac
    11641 atcctaggag aagtaatggg gattgaaagt aatccagacc cagacgcaaa aaagtaacac
    11701 ccgaagaagc agaagaaatg tacttaaacc tatgcagaga gttagtgaaa gcgggatggt
    11761 cactatctga catagaagat aattcctttg aaatgatgat gaaaattgcg tgcacttcgc
    11821 ctaagaaaga gaaacaaaaa gaagttgatc ttaaagactt cttgaaatcc atgtagagag
    11881 gaggtaaaca tatggcaaat ggaaaacctt taggcaatat gattatcaaa ctagacttag
    11941 acagttcagc tttttcaaaa ggtctagccg gagctaaaaa cgctgttaac catcaaatga
    12001 aagcaatgaa atcacagatg caagtaatga actcttcagg aaattctctc ggtgctttac
    12061 aagcaaaata caatggttta ggcggagttc tacaagcaaa tgaaaagcaa gtagaattgc
    12121 tcactaaggc ttataaggat agttttgact ctaacggaaa cgctactgct tcgactgcta
    12181 aatacgctaa tcaattgaat caagcaacag ctagatcagc aagttacgaa tctcagatga
    12241 aaacaactgt tggacagatt gctcggatga aagtggaaac ggaagggttt acggggaaac
    12301 taaaggctaa ttctgatgta ttgattaatt ctgggaaaaa aattgaagcc tttggtaaag
    12361 gagtttctgg gataggatct gccctaactg ttggagtaac agctccaata gtggcaggcg
    12421 caactgctgt tactagtgca gctatttcat gggaaagtgc ttttgcaggt gtaaaaaaga
    12481 caaatgatga agttgtagac tcgaatggaa acgtagttta ttcctatagc gacttagaga
    12541 aaggtctgcg taatcttgcc acccaattac ctggtagcca tcaagaaata gcaaatgtag
    12601 ccgaagcagc cgggcaatta ggtataaaga ctgaaaacgt agttagcttc actaaaacga
    12661 tgatagacat gggtgaatcg actaatatgt cggctgaaac tgcggcaact tctttagcac
    12721 gatttgctaa tattacccaa atgtctcaaa aagactttga taaattagga tcagtcattg
    12781 ttgatctagg taacaatttt gctacaaccg aatcagaaat cactgaaatg ggattgcggt
    12841 tagcgggagc tggtaaacag attggaatga gccaaggcga aattctagga tttgctactg
    12901 ctctatcttc tgttggtgtc gaagctgaag cgggtgggtc tgccttttcg aaggtcatga
    12961 tccagatgca actagctgta gaaaaaggat ttggcgcttt tgatcagctg aaacagatgg
    13021 cagaagaaca aggtgttccg tgggttaatt tagtaaatgc tgtgagagac ggtggaaaat
    13081 cgttaaaagc agtatcagag caaatgggat tcacttcatc tgatttaaag aaaatgtata
    13141 aagaagcgga caattcgaaa agttcgcttg aaaattttgc gaatgtggca ggtgttacaa
    13201 gtgaacaatt ccagaagttg ttcaagagtg atccttcaga agcaatcatc aagttcattc
    13261 aagggctgaa agattcagaa aaacatggta cttcagcaat caaagttctg gatgacatgg
    13321 atataaaaga agttcgacta cgcgacagtt tattgagagc tgcaaatgct agtgacatat
    13381 tcaaaggagc aattgaaaaa ggaaacggcg cgtggaaaca aaatacagct ttaacaaatg
    13441 aagctaataa aagatacgaa acaactgaat caaagctaaa gatattgaaa aacgaagtga
    13501 ttgatacagc aatcgatctt ggaggtccat ttgtagacgc gttgcgtgat ggtctaaatg
    13561 tatcgaaacc attgataaaa aacttaggga atatggcgaa agctttctca gctatgagtg
    13621 atgatcaaca aagaaacatt atcaaatggg ttggattagc agcagcagct ggtccagcgc
    13681 tgaagctttt aggaactggt acttcagtaa taggtaaagt tacttcaact actggaagat
    13741 tgacgaaaag ccttgttgat ttagcagcga aagcagcaga aaagaaggct atggatgcct
    13801 ttgcatcgtc ggtaacaaca gctggtgctg cagctgcaaa gacagcagga gctggaggta
    13861 ttggcgggtt agtccctatg atgggaaaaa cagctagtgc tgcaagtact gctgcgggcg
    13921 ccagcggaat aggcgcaatg acaacgtcac taggtttgtt aggtcctgct ctacttggta
    13981 ttgttggcgt tggtggcgca ctggcagttg gctatggagc atggaaatta tttggtgaag
    14041 aagcttggaa ttcctcgcaa agagtaaagc aatggggaac agatgtcggt gaggagatag
    14101 atagtactct tgacggagta cagaagaata ttgaaggtgc aaatggtcaa ttcggtcttc
    14161 tcaaagaggg ttttactgtt gatgatgcaa ataatatggc ggctaatttc gcaaccgctg
    14221 gaacatcttt ggaaacatct ttaacgggta gaataaaggc attagacgat gcactaaaag
    14281 ggctacctca atcagttcag gatgccatga aagaagtagc tgaatcagaa gaagaaacct
    14341 tagggcaatc tctttctaaa ataaaagaca acaatgaaga aataaaaaaa atcagagaaa
    14401 gagccgcaaa tcagaacaga gacttgactg tttcagaact acaacagata caggatctaa
    14461 tgaaaggaac ttctcaagcg tatgtggata cgctcgatgt ttctgctaaa gacagaaaaa
    14521 agatactatc agccatgaat ggtgatgttg cacaagctac agaagaagaa gcgaagacgt
    14581 ggttacaatc tttagcgaaa caaaaagctg caactaccga aaattataga caaatgcttg
    14641 aagaaaaaaa ggcggcttta aaggaaaagg gagtaagcaa agaattcatt gatgcattag
    14701 aagatgaaat gaatgattac atcaaaacta caaatcaagg attcgatagc caaatagctg
    14761 ctataacaaa taaatatcca caattgatta acgaagtgag tttatcaaat ggtcaattat
    14821 ttagcaaaac ggcagctgct atggacaata ctggtcagta tacaaaagtt tttctcgaaa
    14881 gtaatgaaaa gattttagcc caagcgaaga aaacatcgga tgaaatagca gataatgccg
    14941 aaaaaaatgc agaacaactt ggtatggtag ctgatgaggc tacgcatgct ggatcaactt
    15001 ggaatggctt agttttagac gaaaaaacag gaaaagtaaa aactaatgta caagaagtag
    15061 tgaatgaagc aacgaaggat ttcactaaat ggaatgaatt gaaacctgtt gttcatgatg
    15121 cgaaactaac aacgaatgca aaagatgtca tttccatagc ggcagtacaa aatggctatt
    15181 gggatgatat ggaatggaaa gaaaaagaag cattattaga agatgagtgt tctaaaaatg
    15241 tatacaaggc tttagaaaat gctggtaaat ggaatgagtt agatattcct tctaaagaag
    15301 caatcctaac ttcaaatact cctgaagtta tgggtgaaac tctcatgaat ttgggattat
    15361 gggatactta caaacttcaa gtgaaagatt tagatttgaa tgcgtataaa gtatacgatg
    15421 ctatcgatgg atcagaagaa aaattaaata gttgggctca attagataac cccacaaaag
    15481 aattgttatt agataactca gatttttcta caaaactttt taattccgaa gaaatgttta
    15541 aacgttggaa tcagttatca tctaatgcaa agtttctttc ggctaataat actaaattta
    15601 cagaggttct tttatcatct gacagaatgt ggaatcaatg ggtaaacctt cctgataatg
    15661 agaaaagatt acttgcagat aacgaagatt tagctaccac tgttttatct tcagaagaaa
    15721 tatacggaag atggactcaa ttaccagata atcaaaaata tatgttagct gacaatactg
    15781 acttagcaaa caaaatattc gcttcagaag aatcatggaa tgcatggaaa gcaattccag
    15841 acacagttaa aaacttgctt ggtaataata ttgatttact ggctaaagtg aaagacggga
    15901 ctgtaagtgt agaagaatat aacaaagtta tgccacttct taaaactcta tatggagata
    15961 acgttaattt aaaagaggca atcagtgggg ctaaaagctc tatagagaat tacaacaaaa
    16021 atatttttcc tagtgataaa actgcagtag gacacgatga ggcttcacaa gctgctaaag
    16081 atgcatttga agcgttcaac atcttccaaa caaaaatacc agataaaatt actaaaactg
    16141 tatcagctga ctttataggt cctatgccga aaaatgcgaa aggaacaaac ttccatcctg
    16201 gcggagcagc tatggtaaac gatcaaaaag ggcctactta tgaagagctt ataaccttgc
    16261 caagcggaga aacttttata cctaaaggac gtaatgtcgt tttagactta ccaagaggtt
    16321 cgaaagtact gaacgccact aaaactaaac gtctagtgcc taaatatgct gatggtatag
    16381 gaaacataac gacggtttca tctacgctaa acttagatgc tttaatttta gctattaacg
    16441 agctaacgac ggtattgaga actcaactga tggcaggaac gactgatgct aaagatacta
    16501 aactatcaga tgaaacaaaa gttgccgatc cgattattcc ggacagtctt tctgaaaaat
    16561 cagatcagta tctaggaatt ggagctcaat ggttaactaa tctaatgaat ggttggaact
    16621 cagcggttcc tcaatatatg aatagtgaaa tggtttttat tacaaactat ctcaatgcgc
    16681 taaaacttca aaacaatcct aattatacgc aaggagcaac atggaataag aacttactga
    16741 atggatggaa tagtttaact gggaccttta ttgctacaat caattcattt tgtaatcaag
    16801 caatggtcac gcttagaaac tacaacactc ctatgtacaa caatggccga acgtggcagc
    16861 aaaataatct taatggttgg aattcattgt atggatcatt catagcccgt gtaaatcagc
    16921 ttggtaacga ttcgattaac aaccttcgtt ctaaaagtgg tggtttttac aatgcaggga
    16981 cattcttgtt gcaatcttta ataaacggaa tgaattcgtt aggtaattct ctttctacta
    17041 caatgaacaa tgtcgctaat acaatggttg gtggtatagg caaaggagtt aatggcgtca
    17101 tatcgggagt taactatgtg cttaaggaag tcgaatcaag taagaatatc ggaaactggc
    17161 caattccaca atacgctaag ggaactgatg gacatcctgg tggacttgct atgattaatg
    17221 atcaaaaagg cctagttcac gaagaatatg ttcagatgcc agatggacgt agttttattg
    17281 ccaaaggtag agatcttctt gttaatttgc ctaaaggtgc tcaggtctta aatgcttctt
    17341 taacaaagaa attaaaggaa cgtttaaata ttccacacta cgaaaaaggt attggcaatt
    17401 tagatattgt tgatttgtta gatgatgaaa aaagaatgtt agaattctta actagcaaag
    17461 ttgatttttc aggcattaat gaaccatggc ttgatatgac taaatcagga acgtcattaa
    17521 tgtccaaagc tgcaaataca atgcttcaat caaaattaag tgaattcttc actcatggca
    17581 attttgatgg tgcagtaaat gccaatggtg tttatcaata tttagttgat gtggcacaga
    17641 aagtaatggg taagttcccc ggacttacag taacttctgg atatcgagca ggagatgcct
    17701 actatcacgg aaaacggcaa gctatcgact tggcttatcc aggtatttct ggagatccga
    17761 gatatacagc agcagcaaat tacgcttttg aaaaattccc ttcaaaaatt gcgtatgtca
    17821 ttacgaatgg acgtgtacgt gaccgcatgg gattatctgg aacaaggtca agcggacaat
    17881 ggacgaattg gccagatggc gatcacttcg atcatattca tttaaacggt tcgatggggt
    17941 ctggcgatat attcaatgga ggagttggag gaagtggtgt tgcacgctgg cgttcttatg
    18001 tgtctaaagc attgaaaatg aatggattac cagctacagc agcgtatata aatgcctgga
    18061 tgtcccaaat ccaaacggaa tctggtggta atgagaaagc aatcggcggc aatgatgggc
    18121 tcgccgaagg caatgcaaca ggattacttc aaacgaaacc cggaactttt gcagctaacg
    18181 cttttcctgg tcacggaaat attatgaacg gatttgataa catgttagca gcaatcaatt
    18241 atgctaagaa acgttacgga gttgcaggta tgttgcaagt tatcgggaaa ggacatggat
    18301 atgccaatgg cggcttgatt accaaagacg gactatatcg agcaggagaa ggaaataaac
    18361 cagaaatggt tattccattg acaagaaaaa caagagctat cgaacttatg ggtcaagcat
    18421 tagccttcct ttctggaagc aataaaaaag aaacgaaaag tgagattaat tctgtagata
    18481 atagacatat cctacaagcg atagaaaaac agtctcgtat gacaaatgat ctgctatcat
    18541 tattactctc gttttttaaa agtaacaatc gatcagataa agaactagct ttagatattc
    18601 agaaaatttt aatcaggagg acatagtatt tgaggacagt aatattaaga aataaattga
    18661 atgaagaaat tgatttgtcc acagaagatt attttgctac tgaaatgtcg aatctaggtt
    18721 ttgaagttac aaaagagcat attggacaat gggggagttt cagagaaata ggagagagta
    18781 ttgaattgtc tgaatttcaa tcatcagtta tgatttctgt tcacggattt agagaacaac
    18841 aattatatgg ttcattagtt gaatttctgg ctaatggacc ttttgaacta gaattttctt
    18901 ttgattcaga aacaatgatc aggaaatgta gtttgaaatc attatctaaa acagaaattg
    18961 attcccaaag ctcattgcta acagatactt tgaatctata ttttacttca agctggtatt
    19021 ctattaagcg tgaaaaatta atacagaatt caacttctct caatacacgt ggaaaagttt
    19081 actcatttgt tcgaccatat gtctacacca aaaatgtatg ggaaaagaaa ggtgtattta
    19141 agttcagtaa taattctgta tatttgacaa atagtacaga gcgaatgtct ccgttaaaaa
    19201 taaggattat tggagagtgc tcaaatccat attgggaagt tatccaaaac tctaaagtag
    19261 tagcttcaga tggctatttt attaatatga aagacacaca aatattagaa gtgtctagtt
    19321 tgctagaaga ccaaacggca attttaaaag atgtaggtgg agttgaatct tctgtttatc
    19381 aacaacaaga ttttactaaa tcaaacttcg tccaagcgcc tctaggagaa ttcagcgttg
    19441 tttttcatgt tggacatgca aatgtagaga ttgaacttta tgaggagcgt gatctatttt
    19501 gattttagcc attaccctct ttcaccgaga tatgaattta tataacgaac gtttaatttc
    19561 gactgagttc gaatttggaa cggatgaaat tggagaagaa gtaagtaatt ttattacaga
    19621 taaatatgtc catgtaaaat caggagattt tctactagca aaatttattc ctagcgggaa
    19681 gattgcttat tttggagtga ttagctcgca agaagacaaa aaaataaatt gcaaaggatt
    19741 actcagtttg gccgatagcg agataccgac tgtaaaaata tcaggtgcta attatgaaga
    19801 gcatattcga cgactgattg aatactactt attgaacgat ccaacaaaac aactaaaaaa
    19861 tattttggag gtaaaaacag aaagcgttac ttctcattct tatcaagctg ttgacacaaa
    19921 taagagacga ttgaatgcat atataataaa tgggttcaaa aaatataatg ttaaatggta
    19981 tgtcaaagag attaaaggta aaaaaatata tactggtatt cgagcagttg atgattcaat
    20041 ttatattaaa gataattcat ctgagtttaa tgactgggat gtttttgtgc aatctcctgg
    20101 accagggaac gagaacaaat tattgatcgt agataaagca atgaaagata tagaaaatcc
    20161 cttggtattg tctacgtggt atttagatga cgaaaataat cttacgcaag attcaacgaa
    20221 taagaacatt gcattgccta ccgttaattt agtaaatata tatgatcaaa ctcaagaaga
    20281 tagaccttct tatgaagagg tagctaaatc ggagcttaaa ggcaacgcct attcacatga
    20341 aattaaagtt aatgttgtgc taaatgctag aaatatcaat gtagaaaata tcgaaacagg
    20401 aatgttagcg actattgttt acaaagagca gacttacaaa tctgttctaa ctgcctggcg
    20461 aatatccagc agtaaaaaga atatagaatt gacatttggt aatgtccgaa gtcgttttat
    20521 ggattatttt gaagataatg ggggataaaa aatggttagc aatgtggatg gatatcaatt
    20581 tgaaaacgtg aaagtaagcg cagaaaatga tgctagactt tatcacgttt tatataatcg
    20641 gaaaaatcag gttattgatg gttacgatca gtctatgaat ttatcttcaa gcggattaac
    20701 agtaaaagtt gctgcaggag cagcgattat tcaaggtcgt atggtcgttg ttcgacaaga
    20761 agaaagtata acagttccag caaactcaag tggttatata gcattaacag tggatttgac
    20821 acaagaagtt atacctggat ctattcttcc agaatcggaa gaatatgaat ggactaataa
    20881 tcaagtcaag ctagaattta taacaaaagt tataaaaggt aatttaaaca atggtgataa
    20941 ggtctataat ttaccactat gctcagttaa ttctactgga tcaactgttt caatctcaaa
    21001 gatatcggat agttacgagc tgactctctc taaaggagaa attttgtgga gggggactgc
    21061 gttaatgcat gatactcaaa ctgtccaacc ttcaaaaaaa atttggcaga cagtttcagg
    21121 ttttttgtta gtatggctcc catacgaaaa cgggcaagca attgaggata gatatgtaac
    21181 tacgcctttt tataaggagc gtataacttt tactaatgtt ttaggagaaa ttgtttcagg
    21241 atttgatgac tatcacaaaa agtggtttag taaacgaata aactataatt cgaatactaa
    21301 tatatttaca ggtgcagcaa gcaatgcaag tggagataac gcaaatatgg tgcttaggta
    21361 tatagtttct ttttagttag gaggtatgga agagtggcaa atttagaaat taaattatct
    21421 gcaaataaga gacagcctta tctacgtcac cgtgttgttg gtagagttgg tgatgggggg
    21481 ctcacaacaa tcaatgtaca acttcttgag gaagatgaaa ttacaccttt tgtaattaat
    21541 ctaaacggta ctttgaaatt tgtgggcgaa gtttcaaacg gtaactatac cgagggagaa
    21601 ccagaaataa tcgattcgac taatgggtta attagttaca cgttcactaa gtcaaatttc
    21661 agcacgagtc atcaattcaa acaagcatat tttgaatatg tagatcctaa cggcaaaaaa
    21721 gtaacttttc agaacttcat catagacgtg ttagaacgag tagatattaa ttcggagcaa
    21781 gcgaaatact atatttcttc attggaaaaa ttacagagtg aaatgcaaac cactttcaat
    21841 cagttcatta gtgataaaca ggtccaatac gatcaaatct actcgaaata taacgaatta
    21901 gtaagattga taaatgaatc agataagcaa gtaaatgatc gtattgataa ggcaaataat
    21961 cgtattgatc aaaccaatca gcaaatcggc gatctcggca agctgaaaaa gatgtacagt
    22021 aacagcattg a
//
LOCUS       DARBDV010000044        22026 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000044 DARBDV010000000
VERSION     DARBDV010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.044"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(61..1371)
                     /locus_tag="SCM56_001565"
     CDS             complement(61..1371)
                     /locus_tag="SCM56_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEG1110346.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1383..2801)
                     /locus_tag="SCM56_001566"
     CDS             complement(1383..2801)
                     /locus_tag="SCM56_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1110347.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3041..4609)
                     /locus_tag="SCM56_001567"
     CDS             complement(3041..4609)
                     /locus_tag="SCM56_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEG1110348.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4613..4894)
                     /locus_tag="SCM56_001568"
     CDS             complement(4613..4894)
                     /locus_tag="SCM56_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HEG1110349.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(4891..5814)
                     /locus_tag="SCM56_001569"
     CDS             complement(4891..5814)
                     /locus_tag="SCM56_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HEG1110350.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5811..6689)
                     /locus_tag="SCM56_001570"
     CDS             complement(5811..6689)
                     /locus_tag="SCM56_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEG1110351.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6676..8013)
                     /locus_tag="SCM56_001571"
     CDS             complement(6676..8013)
                     /locus_tag="SCM56_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEG1110352.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8016..9119)
                     /locus_tag="SCM56_001572"
     CDS             complement(8016..9119)
                     /locus_tag="SCM56_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEG1110353.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9193..10701)
                     /locus_tag="SCM56_001573"
     CDS             complement(9193..10701)
                     /locus_tag="SCM56_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEG1110354.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10667..11359)
                     /locus_tag="SCM56_001574"
     CDS             complement(10667..11359)
                     /locus_tag="SCM56_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEG1110355.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11381..12355)
                     /locus_tag="SCM56_001575"
     CDS             complement(11381..12355)
                     /locus_tag="SCM56_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1110356.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12655..13527
                     /locus_tag="SCM56_001576"
     CDS             12655..13527
                     /locus_tag="SCM56_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1110357.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14109..16307)
                     /locus_tag="SCM56_001577"
     CDS             complement(14109..16307)
                     /locus_tag="SCM56_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1110358.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16539..17531
                     /locus_tag="SCM56_001578"
     CDS             16539..17531
                     /locus_tag="SCM56_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1110359.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAV
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17591..19012
                     /locus_tag="SCM56_001579"
     CDS             17591..19012
                     /locus_tag="SCM56_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HEG1110360.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19005..20504
                     /locus_tag="SCM56_001580"
     CDS             19005..20504
                     /locus_tag="SCM56_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HEG1110361.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20543..21937
                     /gene="uxaC"
                     /locus_tag="SCM56_001581"
     CDS             20543..21937
                     /gene="uxaC"
                     /locus_tag="SCM56_001581"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEG1110362.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         6669 a         4493 c         3823 g         7041 t
ORIGIN      
        1 atcattaaaa aaaataaagg tcaagctaaa atattattta agcttgacct tattataatt
       61 tcaacgattg tcaaaagtat tgtttgcagc tgtgttgcca gtttctatag ataaatcaca
      121 gcttttgaaa cataagtttt gtactgcttc gattcgcccg aattcttttg cagttatttg
      181 gcaatgagaa aagcagacat cttctatagg cttttcagga aatgctacta aatcaaaagc
      241 tcgggaagga agtgagtaac ctggagaaag gcatgcctgt acgtttttga tgtgtatgtc
      301 tttgactttc gtcatttgct ttgattcagg tatttcttca gccactgctt gccaactttc
      361 tggcatttga gcaaggtcat tgctattttt tctattgtat tcattatgcc agtccattat
      421 ccaagaaaaa ggaaattgta catcaatcat attcagattt tctaccctga tattttcgat
      481 cactccccct ctttcttttg aagatttcat tcgaatacca cagtctgact gaaaaaaatt
      541 gatatcgtga atatatacgt cactaactcc agcactaact tcgctaccaa tagttacacc
      601 gtatcctgaa cggattttac atctagcaac ctcgattctt gatgattttc gattgactct
      661 tgcaccattg ccatctctgc cagacttaat agcaatacaa tcatcaccac aggccaaatc
      721 acattcatag acacgcacat ttgttgaaga atcaatatca atcccatctg tacttggtcc
      781 atcattgttg cggatgatta ctttttctac taatacatcg ttagaataag tgatttgcaa
      841 gttccaaaaa cctgatttct gaaaaatcaa atcacgaaca acaacatgtt ctgcatgata
      901 cagtaaacaa gcgcgtggac gtttaatagc ataatccgca atccaacgaa gtcccttttg
      961 atcgtattca gctcttgtcc cgcttttttg atcttttccc caatacagct cccaccaatg
     1021 gtctccttga ccgtcgatta ttccttttcc ttctataaga atatctttcg ccgagagaac
     1081 attcaaaatc gctgctggcc atttcatttc tacgccggct actcgtgtat cgatttctgg
     1141 aaatgcttcg atatctgttc tccccttgag gactgcacct tcttcaaaga taagatgact
     1201 cttatctttt aaaaaaaggg ctccgactaa ataaatgcct tttggaataa atacaggtaa
     1261 gtgttctttc gcaccttgat cgattgcttt ttgcagtatt attgtatcat cttgttgagt
     1321 gtcgtttgtt ttaaagttca aagcattgat aatccctttt tttgtccgca atacgtttct
     1381 ccctatcttt cattgatttt tttcaattct tcaccaataa atacagtttt tgaacgattc
     1441 agtgggtaac ctactagcaa aatgattgcg gatacaaata agcagaatgc tggtaacaag
     1501 tttgccatcg tataaatcct ttcttgtact aaaactgatt ggatggctcc acctgttgta
     1561 gacgattggt aaccaatgag tgccagcata aatccgccta ccgctcctgc acatgcttgt
     1621 cctacttttc tcgcaaaaga atttactcca tatactgttc catcttctct taagcctgtc
     1681 ttatattgat gataatcaat cacgtcagta atgaatgccc agaccatcaa attgaataaa
     1741 ccagatccta aagtagcgat aaataaaaga atcagatata cccatgcatt tgttatatga
     1801 aggaaataca tcagcaagta catacatgaa gaagcaagta acgcaaatac gcttgcttcc
     1861 tttttaccaa ataattttgt cagctttgta gcaaatggtg ctaaaactac tactgttccg
     1921 taagtaaata aaagagcaac agacattgct tgtttattat ggaagtaatc attgaataaa
     1981 taagtcatat tcgtcccagc taaaatctga ttgatcacaa tgaaaatgtc aacaatgact
     2041 aaaataatca gtgctcgatt agctaacaaa ccttttacca actgagaagc agatactttt
     2101 tcgtttttga caactcttac tctttcagta gtcaatttac aagttaacat atatgcgaca
     2161 aatgctattg cagcacatcc gattgaaatc caaaagaaat gattccctga aacgacttgt
     2221 tgtccactct tgttatgggt atacattaaa atcggaatga agaatcctgt agttgatccg
     2281 ccaatcgcag aaccaatgct tcggaacgtg gacaacgagg tgcggtcatt aggatttgag
     2341 ctaattgctg ctgccattgc tccatacggg atattgattg ttgagagaaa aatcccataa
     2401 acaatgtatg aaacaaaaat ataagcaatc ttcaccgtca ttggaagatg ttggacgaat
     2461 ggcaaaaaca aaatgacagt cactaagcaa aatggatact tcatttttct gatccaagga
     2521 ataaatcggc cagtttctgt taactctgag acatcacata atcttccaac catcacatca
     2581 gcaaacgcat ccaaaattct cgaaatcaag aataacgtac ctacgattgc ccctgaaacg
     2641 ccaagaacat tcgtataata aatcatcaag aaactattga ctaaacctaa aataaaacag
     2701 ttacctagat caccgaacat gtagcctatc ttatcggaaa tcccaaattc ctttttttct
     2761 ttttttactg tatttgctgt aacagcattt tgtgaaagca tttgaattcc tcctacctct
     2821 tctagtaaac gttttcaaaa ttataactct ttttttcttg tttttatatt cacaatgttg
     2881 gctttttatt gcattatctt gacagataaa aaacaaaaaa taatctaaaa acattgatgc
     2941 ataagattga taactttttt tacataacaa tatatacaaa caaaaaaatt cactaactag
     3001 tgaaaataag ttagtgaacc tcatttatta aagaattaaa ttacagatga tcttttccat
     3061 catacttgaa aaactcaaaa tcagcataac ttcctctttt tttatttaga tcatgcactg
     3121 ctatcccgat aaaatttcca gtaaaccctc ctgagagtat acacatgttt ttttgcggac
     3181 ctactttctt ccattcttca gttgatacgt ctttcaaatg aaactgtgct gtctctttct
     3241 ttacagtgac tttcaaaaaa gtcttctttt ctgtgagtgg aatcgtccaa tcttctattt
     3301 gtgctactcc gttttctgat tctatcatac gtaggcattt tcccttgtcc tcattccaag
     3361 taacataaca ataaatataa ttggattcat ttaagtacag taccagtcca gccatctgat
     3421 taaaattatt aggcatgtat tcaaatgctg tctccgcatc aaaagtaaaa tccgtttgcc
     3481 gaatagctaa caaatgatgt tcgaaggtag attgaagaga ttcaccggaa atcagacgta
     3541 aaaaacctgg tctacatttt gtatcacacc aatcagcatt tggcaaaatg cgtctggcat
     3601 tccagttctt ctttagctct ccttcaaatg tatccataaa ctgatgatct ttttcttgtt
     3661 gtacctcttc atttgtctca atcattattt cttccatcgg agttgtctgg ctatctttaa
     3721 gtcttaacca atcgtcggac gtccaataca cttcttgaat agcggtttct cttcccaaaa
     3781 tagccgcacc gtttaatggt ctggtacaaa gatatgtcat gtaccatctt cctgacgggg
     3841 tttgtactaa gctcccatgt cctgtacatt gcaatgtaga agcaggtttg tcactagctg
     3901 tcagcatagg aaaaccagga tcaagctcgt aaggaccagt aatattcttt gctcggcaaa
     3961 ctgtaactga atgtcctctt cctgtaccgc cttctgccgt catcaaataa taatacccgt
     4021 tacgaaaata caaatgtggt gcctccgttt tcgctaattc tgtacctgca aatattttat
     4081 agatgggacc atcaagcttc ttcttctctg catcatattc ttgtaatacg ataccgctgg
     4141 atttattcgg tgtgtccaga cgataatccc atagttcatt gacaagccat ttttttcccg
     4201 tttcaagatc atggaacaaa gaaggatcaa agccgttact atttaaataa attggttcgg
     4261 accaaggacc tgtgatctca gatgcagtga tcaaataatt gtggacatct ttaaacggac
     4321 ggcctgcact tttgacatct gtgtaaacaa gataaaacaa tccatcagca taactgatct
     4381 gtggcgccca tatacttcca tttgtaggat ttccttgcaa agatacttga gtggtcaaaa
     4441 tatccgttgt atgtgaccag ttgactaaat ttttcgactc atatactcgg attcctggaa
     4501 gccattcaaa agaggagaca ataatgtaat accgctcttt cactcttaat atattaggat
     4561 ctggattgaa tcctcttaaa ataggatttt tcactttgat agtctgcatg attcaggctc
     4621 cttttctcat tcttactcga tataaactga atgccccagc tgctaaaaaa gcaagtgaaa
     4681 taaacaacac aacggcaaac agaccagtca tgtcctgtaa taatccaaat gtattttcta
     4741 aagacaaagt tgaatgatct acagcaagcg actgataact ttttttaaac tctgacgcag
     4801 acatattact taaagtaact gctaacccgc caaagaaaat caaagataga ctgccaccga
     4861 ccattcctag caatagttcc cattttcttt tcatttctga accccctgct tttctaaatg
     4921 tatccattca ggtcttttga tcgatgtttc ataattgtta ttacgttcag agaatttgac
     4981 agtctgttca tttttcttgt catcccaatt atcccaacca gttgggtgaa catgcccacc
     5041 tatcttacac gcttcaaata agacatttgc atacggacgc cacggtcttc ctaaatagac
     5101 gtttctagta tcaggttctg cagtcaaggt acagctttca aaacgatacc ctgttttttg
     5161 atttttcggc gtagatgctg ctgtgatgta tccaggattt ttactggcta agctatggat
     5221 atgacactga tgaaaaacgg catctgcccc accaaatatg aaatccaccg ttccttcaat
     5281 ccagcaatat tcataggtct gacgacaata ctcaggattg gcagaagccg gtgtacaaaa
     5341 aggagtacca tctttttgca aatcaggcaa cggacccgtg cataaggtat cctgataacc
     5401 agatagtcga cagtttttta aagatacgcg atctcctgaa ttgaataaag caacagcctg
     5461 tcctaccttt tctcctggac ctgctgtatt ttcgatgtga agattttcta atacgacatt
     5521 cgatccttct aagaaaaaag tagcacttcg gaaagtccct aactggctac cgtcaagata
     5581 ctcctgtttt gcaaaacgat taccggagat aactactgtt cctgctccca caagtttaaa
     5641 attggataat ctgctttcta tatattcatc atatcttcct tcttttacta tcaagacctt
     5701 tggatggcta tccggcaggc ctgccaaata atcgattcct tcttggatag tttcaaaatc
     5761 cgtcggggta tctatagcat tctttccaat tatcagtttg tctttcatcc tcataaatgc
     5821 tcccttatga attgtttcca aatatttctc atttcttgtg tttccatatg accgcctgtt
     5881 aaagaaaaag aagaaaagtt ttctggacta cccattgatt gatacttttt gtccagccct
     5941 tttctcagct tcagtacgcc ttcgtccaag cacatcgtat cgttttttcc aactaagcta
     6001 agacgagggc gcggagcgat tttttcttgg attgctaacg ttgtataagc tgtcaataga
     6061 tttggcacat agtaataaaa cccatgatga tctaggcggc gatgtagcaa aagtgtttcg
     6121 atgtccacct gtgccgctag atcaatacaa actttgacac gagaatccaa tgctgtcacc
     6181 caccagctta ataagcctcc catagaaaga ccaatcgttg cgagccgttg agtatctgtg
     6241 tcttcccttg tctccaaata atcgatcaag ctgatcacat cataaatacg cattccccat
     6301 aacgtttttc ctgttaataa aaattgtttg aacaactcgc tttcagaaat acctccgcgt
     6361 tcttcaaatc cccaagcatc aatcgaccaa acagcatatc ccattcctgt cagtacttct
     6421 gcaaaagatg gagaagctaa gtaaggagca ccatggatca attcactttt cccgactgaa
     6481 aaatctccgc cgtgagaatg caggtaaagg accgtcggaa acggtccttc tttttctgga
     6541 taagcataga gggctggtac tgtttccaat tcatttaagt acaattgata gtattcaagc
     6601 ataaaaccgt tttgtttttc tcggtataat cgttttccac taggggtatg tctgctagat
     6661 ggagcaccta aaaaatcatt caactgcata agcaaagttt ttacgaacaa ccagttcatc
     6721 catattttct gattgatttg attggaattt ctcaatactg atttctttag tattttctaa
     6781 tagaaatgct ggtccctcgt gattttcaat cgacatctgg ttaaaggaaa ccttacccgc
     6841 aaagccaata taaaatcctt gtctgctcat ttcctctatt cctgtcatca tagcaggaac
     6901 accgggaact gcatcttggg ccatcgagac atcgatattg tcaaatgtga tttcttgtat
     6961 gaattgttcc gctaatccat aaataaaacc tgcagaagca tggacattcc ttgccgtgat
     7021 attcgaaaaa tggatccttc tgaatgctga tgtccgttca tctatcggat aagccttttt
     7081 ttcccataca tacggttctt tccctcgcgg accacagaaa taataaagat tcagtataaa
     7141 tggacacatc acattatcca tgactatatt ggatactcga atatcttcaa cgatcccgcc
     7201 tcgacctcgt ctagatttca atcgtattcc tcgatcagtt tcttggaaga tacaatttga
     7261 aatggtaata ttacgaatac taccactcat ttcacttcct aatacgacac cgccatgtcc
     7321 atggaccatc gtgcaatttg tgatcgtgat attttcgcaa gcgatccttt cgtacgtatc
     7381 ttcagtccct gccttgattg caatacagtc atctcccacg tcaatatggc aattactaat
     7441 acgcacattt ttgcatgatt ctggatcaat tccgtctgta tttggtgaat ccgctgggtt
     7501 caagatcgtc aagttatcaa acgttgcatt actgcaaaga atcgggttga tcgtccaact
     7561 tggtgactgg atcaatttaa tatctttcac agtaattcga tgacagttat ggaaactcat
     7621 caatttagga cgaggataag cgagattatc tggttcattt ctaaaagtat gccaccattt
     7681 ttttccattt ccatctaatg taccgaaacc agtgacagag atgttctcta cattttgagc
     7741 atagatacag gaagcataaa cttttcgatg gactccttcc caacgtgaat gaacaacagg
     7801 ataatctttt ggatcgtcag agaatttcag aaccgctcct gcgcttaaat gaagttcgac
     7861 attgcttttt aaaaataaag ctcctgttag aaattcacct gctggaacaa ctacggtccc
     7921 gcctccgtca cttgctgcag catcaatggc ttgttgtatt gcttcagtat tcaattcatc
     7981 aattgatgcg ccaaatgtca aaatatcata catatttata atgcctccac ttcaatcaat
     8041 gcttgcaaga atgcaccaac gcctttttga tcattacaaa taattggttc actgatgtag
     8101 taagcatatg aaccatctcg ctgatctgct ccgcctaacc ctgctacttg acagttttta
     8161 ttcaagttca gccagccttc ttttgtttcc agaacaaatt catcaagtaa tccttgatgt
     8221 gatttcttca cttgtggaag ccattcttct ttgttcagca gtcccaaacg tattccttta
     8281 gccattgcgt aaacaaacat actactgccg gaagcttcca gataatttcc tttccgtttt
     8341 gtttctgttg ttacttgata ccatgtcccg ctgttttctt cttgatatgg aagtaatgca
     8401 gccagtgtct tttctagaat ttctttcagc aaagaacaat ctgtttcttc atgaagtaat
     8461 tcataggtat ctgctgcagc catcaaatac cagccaatcg atcggctcca aaaatgatgg
     8521 gatagacctg tttttggatc agcccatggt tggaccttct tttcatccca tgcatgaatc
     8581 agcaaatttg tttttggatc gaatagatgt ttataactga tttcaaactg tctcaaaaca
     8641 tcttggtaat ctttttcttc actaaaattc tggataaatt cagcataaaa aggagaaccc
     8701 atatataagc catccaacca aatttgataa ggataaattt ctttatgcca aaaagctcct
     8761 tcggatgtgc gtggatggtt tgccaactgc tgggaaagga gttctgctgc ttttttatat
     8821 ttttcttcgc ctgtttcttt gtacaataaa aagaaaagct ttcctgtatt tacatggtcg
     8881 atattgtatt cttcaacgct gtagccacgg atcgttccat cttcttgcac gaaatagtcg
     8941 atattgtctt ttatgaaatc aaaataaatt ttttcttggg ttcgttccca aagtaattgg
     9001 aaccctttaa gtacgacgcc atagtcataa gaccattttc ctttatacca cttttcttca
     9061 tataagcgag gtgttctttc catgatactt tttgctaatt tctttgacca gttttccatt
     9121 atttgctctc ctttaaaaat taaaatagga tttgggacaa ataccaagaa gtatttgtcc
     9181 caatcgtcta tattactgtg ccgctgcttt gtatgcttcc tcgtactctt gtgtgattcg
     9241 atcgcctcct tgtgctttcc agtcttctac agcttttttg aatccatctt cgtcaagctg
     9301 tcccatgatg tattgcaccg tagcatcaat gacgattttt tcaatttctg atcctacttc
     9361 attgttcgtc ggtgaatcca atggcacaga aggatccatt acagcaaatt cttcattttc
     9421 tttgatcaat tgattagaga attctttttc agggtctgta tctttgaaag agaacgtcac
     9481 ttcactagga cgagagcttg ataacggttg cacctcttgt tgccaaagat ccatatcgat
     9541 aaattcgata gaaccatcat cattcaattc ataatgcgtt ccttcgatac cgccagtcat
     9601 caatttatac acatcttctt ctaaaagatc atcaacaaat tgaagtaatt tcttcaattg
     9661 tgcttcgtct tttacttgag atttgggaaa tgctagcaag ccgccaatac catttccttc
     9721 tgaccaaaca tgataaccgc cgccttcacc atctgagatc ttgtttacag gcactaattc
     9781 catatcatct tgaatacctt gacccatcgt cttcagattc ttgatatcca ccattccagt
     9841 gtagattcca gttcttcctt gtgcaaattg ttcttgctga tcagtctttt gggtaactgc
     9901 aaagtcttgt gccaaatagc cattctcaaa tagatctctt gaaaaattca aagctttcat
     9961 atactcttct gtatcaaatt ctggagtgaa tttgccatta tcgtctacag cccagccatt
    10021 tggtgctcca aaataagaag tgaaaagttt aaagctggta taacgaatat cactaggcgc
    10081 acgatcgcca aaaccaactg tgtcgtccac accatttcca tcaggatcgt tttctgtaaa
    10141 ttgtttagct acttcataaa gctcatccat ggtagttgga acatctagtc ctagattatc
    10201 caaccagtct tttcgaatga ctaagccagc acgagcgtag tctttttgta tcggtacgcc
    10261 gtacaaggca ccgtcgatac tcgcagcttc gattctatct tcagaaattt gacttaaatt
    10321 tttatagtct tttaaatatt ctgatacatc ccagaatacg cctgatttta acgcattgcg
    10381 gacagaagag tttttcaaca tcgcaatttg aagtgtaacg atatctccta gttcatcaga
    10441 agccaatgca gttgttaatc tttcttcttt tgaagcatca ggaatccagt tgaatgtcag
    10501 ttccgtatcg gttttttctt cgatcaagtt cacaatcgtg tctgtaggcg gtgaagcatg
    10561 atgcaaaata tttaaccaag tgattttagc ttttccatct tctgtctctg aagaactgtt
    10621 acttgaactg ctgccgcatg cggcaagtgc tagaattcct gcaaaactaa gacctaattt
    10681 tacgagcgtt gcttttttca taaattttct ccttcactgt tttaataata tttttgtttt
    10741 cttctgtttt ttctgtatat gagttcttcc attccaaatg gcaaaatata gaatttgctg
    10801 tccacgtgac tgcggaaaag aagagactgc caccgataaa ggttaaaaca cctggcattg
    10861 tttggatgat catcagataa atccctacca tacttaatat caacactaat gagtaatgaa
    10921 gatgtgagaa tccgaataaa aatgccattt tcattttgaa aaaatatcct ttccaatcat
    10981 aatgagccat gattgggaaa atatagagtg ctgagagcac atatatgaac gcagcaatat
    11041 acaaagcgat cctaagatac caattgtgta tgaaagaaag atccacaagt aaaacaatcc
    11101 cgatggcgcc atatatccag ctcatcagca aggtttcacg atagttttct ttaaaatatg
    11161 tgaagtacgt tttcgtcaaa ggaaacgttt cttttgagcg cagccatttt ctaatcactg
    11221 caaccatcgc atacgatgca ggaccgaaag taaaaactcc caaacctgcg atgactaaac
    11281 cgccccacag aaaattcaaa taaaccaact gtaaaatacg atttagcctt ccgtttattt
    11341 tatctaatga ctgcagcata tctttccctc tctttctatc ttagtaaacg ccgtcttctc
    11401 ctagcttttt ggcaatctta ttagctaaaa ttaccataaa caatcctacg acccctttaa
    11461 acaatcccat tgctgtactg aaacttaact gtccattctt caatccagct gtataaatat
    11521 aggtgtcgaa aatttctgca acgcttcgat tcaatgaatt caataacaag tacatatgct
    11581 cgaaccctaa gtctaatgtc tgaccgattt ttagaatcag caaggtaatg attactggac
    11641 gaattgccgg caatgtaaca tgccatgttt tgcgtaagcg tcctgctcca tccatttctg
    11701 cagcttcata caactgtgta tcgactgcag ttatcgctga aaggtagatg atcgtagacc
    11761 agcccaactc tttccagatc acttgtccga tatatactgt gcgtgtccat tcaggggatg
    11821 ttagaaaact gattttttca aatcccattg ttgcaaggaa ttcattcaat acccctccgt
    11881 caacattcaa aaatacataa gtaatcgaaa caatgattac ccaagacatg aagtgaggga
    11941 tgtaaatgat cgtttgaatc ccgtttttca atcgtttgtt cgttacttca tttaatagta
    12001 aagacaaaat gattggcatc ggaaaaaaca cgaagatatt taatccgaac aagatcaacg
    12061 tatttcttaa taatgtaaag aatgtcggtt ctgtaaacaa acgaatgaaa tgtttgaaac
    12121 cgacccaagg acttcccatt attcctaaaa atggttgata atcttgaaaa gcaataacga
    12181 gtccccccat aggaagatac ctaaaaatca agaaataaag gactcccggc agaatcatca
    12241 catataaaaa tttattcgtt ttgattcctg caattatttt ttttcgtctt ctgagctttt
    12301 cttctttttt cgatagcgtc agttgaaatg gttcttgttt ctttacactt tccataagcc
    12361 cctccttagt tttgtgaatg tttgctgctt atgacttcat gatagataac tatctgaatt
    12421 acgtatataa tcatttttat cgtaagtaaa aacgcttaca tagcaatgat ttttgtgcta
    12481 tacaaaacga taaagtgtat tttttcacaa ataaactacg catagcaaac cttctatttt
    12541 tttctatctg acacttagca taggaaatga ttatatacgt aatctttcac cgatgataaa
    12601 ttgaacatat agaaacagaa acatttaatt tattttgagg aggagcctat ctatatgaaa
    12661 aagaaagtct ctataggaga aagaagtttt acacttttca acaatacgtt tctaattttg
    12721 ctagctttga tctgtatcgt tcctttttta aatatcatcg ctacatcttt tgcttctaca
    12781 caagaagtcg tcgcgaaaaa gtttatcctt tttcctacta ccttttcttt agacgcgtat
    12841 cgctacattc tttctacgcc gaccattttt agagcacttg ctgtttcgat tggtgttaca
    12901 ggtgtcggaa cgatcgtcag tatgtgtgca acttcactga tggcctacgg tctttccaga
    12961 aagtatttgt ttggtcgagg tttcgtcaac tttcttgttg tattttctat gttgttcagc
    13021 ggggggatga ttccaacgtt cttagttgtt cgttctctag ggctggtcaa ttcttactgg
    13081 gccatgatcc tgcctgtggc tgttaatgca atgaatatga tcattatgag aaactttttc
    13141 caagccttgc cggatagttt ggaagaatca gcaaaaatgg atggttgcac agattttggt
    13201 gtatttttca aaattatgct gccattagct ttaccgtcta ttgctactat ctcgcttttt
    13261 tatgctgtta cttactggaa cacttatatg actgctattt tgtatatcaa cgattcatct
    13321 aaatggccga tacagattct tttacgccaa atcgtcattg tatcaagtgg gatgcaagca
    13381 gaaagtagtg ccgttgatgt gattccacca gcacaaacag ttaaaatggc tgttatcgtt
    13441 attgcaacag tgcctatgct gattgtttat ccttttgttc agaaatattt tgtcaaagga
    13501 gcactcgtag gttctgtcaa agggtgatta tgtactgaac gcaaaatggt agttgtgtca
    13561 gaagtggtca gcttcaagaa ataagaaggc atttacgaaa attgcttctt aaatttttgt
    13621 gaaatttcgg cttatttttc gaagaatcta ctcctggaac accgtgtatc agaggttgtg
    13681 aaagagacgt tcagccttga gcgatgataa aaactctcac aaacattgcg acgagtaacc
    13741 cacaggagca ataagaagcg attttgaaaa atagcttccc atatttcaca aaattatgcc
    13801 ttattgtcga tagcctgtct cttgaacgcc atttattcgt aaaaaagaac tgtgacaaga
    13861 cttttgtcac agttcttttt gcgtgcttag ccttgaaatt taactatcca taaatacaaa
    13921 tactagggtt attctaactt cttttattgc ttgaatcagt ccacaagaaa aacagcctct
    13981 tgattaaacg gcgtttatct tgttgattct gcggcaataa aatcagccag cagcaaaaca
    14041 tgaagtgctg ttttttgctg tcctctttta ttgcttgaat cagatcacaa gaaaaacagc
    14101 ctcttgattc acaaatattt ttttcgatat tctcccggcg taatcgattc cttcttttta
    14161 aagaaacgaa taaaattttg cggatttcga tattgcaatc tttctgcaat ctcttttacg
    14221 gagaggttag tctctttcaa ccattttttt gctacctcta aacgatgatt ttgaacaaaa
    14281 tcaccaaatt tttctcctgt ttccttttta aatacattac ttaaatagtt agggttataa
    14341 tgcaacctat cagcgatcac atctaaagac agatcacgat catattctgt gtagacgata
    14401 tgcataatat tctccgacaa atttttaaat tcatgatcag tgattgcttg agagcattct
    14461 gtgattggca gaactaattg ttctaaaaca agtttttcta gttctttggg atgataattg
    14521 ttcagtgctt ttacataaat ttgtttcatg ttatcaaagt tttttgaatc tactcccaat
    14581 aactgtccta attgaacgag ttcattgatc aagcggaccg aaactacttc ccgactcaaa
    14641 ggatttttat tttgtgcaaa caatgcatca atcaattcat gtactaaatt cttcacttcc
    14701 tgatttccag aacgaatggc ttcaaacaat cgattttgtt cttctatcgg atatctaata
    14761 agtgtttttt catgagcagc aggaacgatt tcctgataaa aaataattga acttggacca
    14821 gtatttacac gataatacag ggcttctttt gcccgatcaa ccgattgctt gctttcattc
    14881 agcgtttcga accggttact gatgcctata ctgatagtta aattcaagta ttcttttaca
    14941 cgtttttgaa tgagtcggca atactccatg acttttttgc ttgcttctgc atcatcttta
    15001 tccattcgat aaatagtggc ttgcatttct tcattcaaaa cgatcggcag cattcgattt
    15061 tcctttggca caatttccgt gatcatgttg ttgatcgcta aaagtagcaa atcacgctcc
    15121 ccaccgtgac tatcttctaa cagatcgatt tgaaccaaag ctgtataata gcagtcattt
    15181 tgagaactat aaccaaattg ctgcaaccgc tgatctagtt ctcgcctttc tactcgattt
    15241 ctaaataaac taagtacaaa tagcgtttcc agctgtggct tttgggtaac taagtgtgca
    15301 cttaacgctt ctttttctcc tatgatccga ctgactgatt gagcgaccaa atctagttct
    15361 ttcccactga atccttttga tttaagattc agacgttcct gtatttgaga aatcggacgg
    15421 gcaaatctgt ccgaaaataa gtaggaaaaa agaccaatga gtcctaccag tgttaaagac
    15481 actgcaacta gcccgattct cagattgcgg atcattgcgc caattgcact agagtcaact
    15541 tctaatctgt aaatccaatt attataatct gatttcagta ctatatacgt tttatctttt
    15601 tctcttatca ctttcagctg attgtaatct agatccacgt ccgttaatac agttggttgc
    15661 tctttatttt ttactgtctg agtagtaaaa aggttgccaa cttcgttttc aatagaaagt
    15721 atgtcttctc cactttcacc aacaacttgt tctattgtat gtttctctat cgtagcaatt
    15781 cccaatgcat atttttcttt ttcaaacatt ggcaaagaaa tcaccatttc gattcctttg
    15841 tttttctttt gccaaaataa attatttttg ttatttatgt acttctgttg atacttttca
    15901 acttcctctt cggtcagttg actcaacgat ccattgatta ctccccattt accagccaaa
    15961 ctgactaaat cataattatt atcatccata acaatcgttt ctataaaatt tagttctttc
    16021 ctgatttcag aataagtgga gtaatctgta tatgtcagcg gatgcttgaa tatgtccgag
    16081 aaatttttgg tcgaaccata cgtagaaaac gaatattcaa tcgttctaat tttccattca
    16141 acgttagttt taacttgcct gagcaaattc tttttgtctg ttaaataact cgattcgatc
    16201 gattcatagg tattctggta aatgaataaa ctaaatccta tgacaagtac ggctcctaat
    16261 aaaaacatcg gtaaaaatat tttataaaat actgtacttt tcttcatttt cttcctctcc
    16321 tccttttaaa ataaaaggct ttcatttttc tgtaaaattg atactcatct aattaaatga
    16381 gcattccccg ctttcaagaa tagcataatt aaaaataaaa atccaagtaa tttttaacgt
    16441 gtgcaatctt ttcatatcaa agatctattg acttatcgta gttatcgtaa tatgatgaat
    16501 gcgttaacaa aaagtgaaaa agtaaggaaa atgatggaat ggtaacgatc aaagatatcg
    16561 ctaaagcagc tggcgtttct catacgacag tttctcgtgc tttgaataac agcagtttaa
    16621 taaaaaatga aacaaaacaa aaaatacgac aactagctaa ggaaatgaat tattcgccaa
    16681 actatagtgc aaaaggttta gtgaatcaaa aaaaatatct aatcggacta tttttctcaa
    16741 gtttgcataa aggaacttca tcaaatttcc tagcagattc cattgcaggc atccattcag
    16801 tattagacac aaattacagt ttatctgttg aaagtatcga tgttgttgat ttaacagaaa
    16861 tcaacctgca acgctacgat ggactgatcg ttatgagtca aagtgatgat gatcagcctt
    16921 tcatcgacta tctcaaagaa aaagaatttc ctttcgttgt tgtcaatcgt cacatcaatg
    16981 atccagcggt tatcaacgtc actgccgatg atgcggtcgg tgttatgcag gcagtagatt
    17041 acggtattgg atgcggacat acgaagatcg cttatattgg cggaaagaaa aattttcgtt
    17101 cgacaaaaga acgggaaaaa ggggtattga aaagtctaaa aaaagcgagc atccctgtca
    17161 atctagccta ttttttaaca ggagattaca gcttagaaag cggcctccaa aacatggaat
    17221 acctattatc actttctgac ctgcctactc tggttttttg cggcaacgac gacatagcga
    17281 ttggtgcatt gagagctgta catttagctg gcctccgtgt tccccagaac atttctctga
    17341 tcgggttcga tgattctcca gtcgtctcct atctcaatcc gccgcttacc accgtacgta
    17401 agcctttgaa agaaatgtgt caagaaggaa caaagctttt actagatttg atcgaaggca
    17461 ggacgatcga acaatctaaa attgagctct ccaccttgtt gatccttcgt gagtcgatca
    17521 attacttgta aactacaagt aattttatat aaaaatgtta acgtgtgcat aacacagaaa
    17581 ggaagtttat atgaaaaaac tgacaaaaga attagtgaaa aaaaacgaag cacctgtaaa
    17641 agttcttcag tttggtgaag gcaattttat gcgtggattt attgattggc agattcagca
    17701 gctgaataac caacaattgt ttaaaggtaa tgttgcaatc attcaacctt tagaacaagg
    17761 tcttggaaat atgatgaacc agcaagataa tttatatacg gtcattttac aaggactcag
    17821 caatggcgaa atcgttgata cttccgagat tatcaccgta gtagaacaaa tgatcaatcc
    17881 ttatgatgaa tgggatgctt atctatctct agcagaaaat gacgacttgg cctatatcgt
    17941 ctccaataca acagaggcag gaatccaatt cgtggagact gataatttaa cagatactcc
    18001 cccacagagc ttcccgggca aactgacagc ctttttatac cgacgtttca aattaaacaa
    18061 agctggcttt accatcattc cttgcgaact gattgacaga aacggcgaac gtctaaaaga
    18121 aatcatttta cgctacgcag aaaaatggga tttagaacct gcttttgttg agtggttaga
    18181 aaaagaaaat gttttctgtt gtagtttagt tgatcgcatc gtcccaggtt atcctcgcga
    18241 agatgcaggt gttttcaatc aacggcataa ctatgaagat caattgatgg tcaaagcaga
    18301 accattcatg ctttgggtga tcgaagggcc taaagaattg gagaatcaac taccattcaa
    18361 acaggctggc ttgaatgtca tcatcacaga tgatatgact ccttatcgtg aacgtaaggt
    18421 gcatttgctt aatggaccgc atacagcgat ggtccctctt gctttgttgg cggaattgga
    18481 aactgtagaa gaagtaatga aagatccttt attctccgca tatgttgatc agttattcaa
    18541 tctagaattg attcctatgc tttctttacc taaagatgaa ttagccattt atgctgacca
    18601 aatcaaagaa cgtttcttga atccttttgc ccatcacaaa ttagaagcga tttcattaaa
    18661 tagtgtttca aagttcagca cccgtctgct tccagtgttc aagaagtata tcgaagaaca
    18721 aaatcaggta ccaccgttaa tcactgtttc actagctgcg ctacttctta tgtatcgagg
    18781 cgatcaagta aagccacatg atgatgaaaa gacgataagc gaatttactg atgcttggtc
    18841 tgataatgga acagctattc ctcgattatt gcaaaacgct gcgctttggg gagaagattt
    18901 gtctcaaata ccgaatgtga cagatacagt tcaagaaata gctgcaaaga tcgatcaaaa
    18961 aggtgtccgg tcagtattac aagaaatgaa aggaacgatg gtccatgctt gaaaccctgc
    19021 aaccaacaat gaaattaaat cgacgagaca gtgtggtcgt cgcattaacc cctattccaa
    19081 aacaaacaca actgacgatt gatcagcaaa cgatcaccac tcttgaagat atcccgcaag
    19141 gtcataagat tgcattgggt gatttgaaag ctggagacaa cgtgatcaaa tatggctatc
    19201 caatcggtca tgtgacgaca gacgtaactg ccggtcaatg gctgcacact cataatgtaa
    19261 aaaccaatct ttctggtgaa ttggattatc gatatgaaaa agatgtccat ccaagtcatt
    19321 acctttttga gaaccgaaca tttcaaggtt acttgcgaaa aaatggaaaa gtagggattc
    19381 gtaatgattt gtttttagta cctaccgttg ggtgtgtgaa tggcattgct gaactgatcg
    19441 tcaaacagtt caaggaaaaa catccagatc tgggacagtt tgaccatatt acgattttaa
    19501 agcatcctta tggctgttcg cagcttggaa aagatcatca aaatactcga gaaattttag
    19561 cggacgcagt aaatcatccc aatgccggag gagttttagt atttggatta ggttgtgaga
    19621 acaatacagt tcctgaattc aaaaaaattt taggtgaata tgatgaagaa cgagtgaaat
    19681 ttctagtggc tcaagatgtt tacgatgaga tcgaacaagg tgtggctcta ttagaagagc
    19741 ttttaacagc ggcggaaaat gaccagcgca tatcagtccc gctctctaag ttgaatgtcg
    19801 gtttgaaatg cggcggttct gatggtctat cgggaataac tgctaatcct ttacttggtg
    19861 cattttctga ctacttgatt gctcaaggag gcagcacgat cttaacagaa gtgccagaaa
    19921 tgtttggcgc agaacaagta ttgatggcac gagcggaaaa tgaagaagtt tttgactcaa
    19981 tcgttgactt gatcaatgac ttcaaacagt atttcttgtc ttacggggaa ccagtttacg
    20041 aaaacccatc tcctggtaat aaagcaggtg ggatcactac attagaagat aagtctttag
    20101 gctgcacgca aaaatcagga agctctcctg tcgttgatgt gctacaatat ggcgaaaaaa
    20161 tcagaaaacc agggcttagt ttattgcaag ctcccgggaa tgatcttgta gctgcctctg
    20221 ctcttgcttc atccgattgc cagttggtct tgtttaccac aggaagaggg actccgtttg
    20281 gctcatatgt cccaacggtc aaggtatcta cgaatacaac tctttttgaa cgcaaaggtc
    20341 attggatgga ttttaatgca ggcgtcttat tagaacaacc aatggaagta atcttagaat
    20401 cgtttgttca aaaaatcatc gctgtagcta gtggagaaga aacgaaaaat gaacaaaatg
    20461 acgttcgaga aattgccatt tttaaaaacg gagtaacact ttgattttca taaaacagta
    20521 taaaaagaaa gaaggaagat ttatgttttt atctgatgat tttttattaa aagacgattg
    20581 ggcgaaaaag ctttaccatt cctacgcaaa aaacatgccc attattgatt atcactgtca
    20641 cttagatcca aaagaaattt acgaaaacaa caatttcagc aatctgacag aagcttggtt
    20701 aggaggcgat cattacaaat ggcgcttgat gcgagcttgc ggcgtgcctg aagaaaaaat
    20761 cacaggaaat gcttctgatt ttgaaaaatt tttagcttgg tgtcaaaccg taccaaaaat
    20821 tatcggaaat ccgctttaca gttggtcaca tcttgaattg aagcgtttct tcggtattga
    20881 cttgttgata aatgaagaaa atgcagagaa aatttggaca caagcaaatg aaattttggc
    20941 agctcctgat tttcgccgtc gggaaatcgc aaaaaaatcg aatgtagaag tgatttgtac
    21001 aacagatgat cccgcagacg acttgcacta tcatcagttg cttgctcatg aagaaccaga
    21061 tttgaaggtt ctaccttcat tccgtccaga taaagcactc aatattgaaa aagaaggatt
    21121 taatgcatgg gtcacactac tggaagaagc agctgaccaa acgatcacca cctatcaaga
    21181 actgatagaa gtcttagggc agcggataga atacttccat caacatggat gtcgtgtttc
    21241 cgaccacgct ttagacatct tacgctataa agaagcggat gaaacagtat tagaagagat
    21301 ttttcaaaaa gcacgagcta acaaactatt atcttcagat gaaatcgatg cttatcttac
    21361 agaaacactg atacatttga cccacttcta tcattctcat aattggacga tgcagctgca
    21421 catccatgct tatcgtaact gtaatacaca aatgttggaa aaattagggc ccgataccgg
    21481 ctatgatgga atgaatgatc tttctttgac tattcctctg caaaaattat tgaaccgtgc
    21541 agaagaaacg gatcagttgc caaagacgat cctttattca ttgaatccga atgactatcc
    21601 tgcgatttta gctttgatgg gctgtttcca aaaagaacag aatggaaaac tgcagttagg
    21661 ttctggatgg tggtacaacg atactcgagc tggtatgcgc gatcaattga cacaatttgc
    21721 tgatggtagt gctttgggga attttgtcgg catgctgaca gattcaagaa gtttcctgtc
    21781 ctacactaga catgaatatt tccgtcgtgt tctttgtgaa tttttaggcg aaatggtcat
    21841 gcgaggagaa gcacctgaag atgaacaact tttaggaagt ctagttcaag cgattagtta
    21901 tacaaatgct aaaaattact ttggcttttt tgcctaattg attaatagta acagtaacga
    21961 atcaaaaaca ggatagtgaa ataacggaat ccgtttatcc acgttttaga gaagaagacg
    22021 gaccaa
//
LOCUS       DARBDV010000045        21988 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000045 DARBDV010000000
VERSION     DARBDV010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.045"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..395
                     /locus_tag="SCM56_001582"
     CDS             <1..395
                     /locus_tag="SCM56_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110363.1"
                     /translation="ISLKDESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLNQKTIKKLNIGG"
     gene            398..1147
                     /locus_tag="SCM56_001583"
     CDS             398..1147
                     /locus_tag="SCM56_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1110364.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1159..1428
                     /locus_tag="SCM56_001584"
     CDS             1159..1428
                     /locus_tag="SCM56_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110365.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1433..1597
                     /locus_tag="SCM56_001585"
     CDS             1433..1597
                     /locus_tag="SCM56_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110366.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            1590..1892
                     /locus_tag="SCM56_001586"
     CDS             1590..1892
                     /locus_tag="SCM56_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110367.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1889..2050
                     /locus_tag="SCM56_001587"
     CDS             1889..2050
                     /locus_tag="SCM56_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEG1110368.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            2047..2352
                     /locus_tag="SCM56_001588"
     CDS             2047..2352
                     /locus_tag="SCM56_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110369.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            2352..2708
                     /locus_tag="SCM56_001589"
     CDS             2352..2708
                     /locus_tag="SCM56_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HEG1110370.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            2695..2913
                     /locus_tag="SCM56_001590"
     CDS             2695..2913
                     /locus_tag="SCM56_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110371.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            2910..3329
                     /locus_tag="SCM56_001591"
     CDS             2910..3329
                     /locus_tag="SCM56_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HEG1110372.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            3326..3883
                     /locus_tag="SCM56_001592"
     CDS             3326..3883
                     /locus_tag="SCM56_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEG1110373.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            3880..4176
                     /locus_tag="SCM56_001593"
     CDS             3880..4176
                     /locus_tag="SCM56_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110374.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            4253..4666
                     /locus_tag="SCM56_001594"
     CDS             4253..4666
                     /locus_tag="SCM56_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HEG1110375.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            5012..5386
                     /locus_tag="SCM56_001595"
     CDS             5012..5386
                     /locus_tag="SCM56_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110376.1"
                     /translation="MEDKMYLRQKVFTKKDIENQINGFIQKLENANSYLINKELNKAY
                     DQWKEVYDELKLIQNETKLVRTEKKNENSFFFDGYAILMLETVAKQNIKAPKKELSDN
                     IDNALAELRYYVMQIKDVRITR"
     gene            5423..5590
                     /locus_tag="SCM56_001596"
     CDS             5423..5590
                     /locus_tag="SCM56_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110377.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKH"
     gene            5616..5960
                     /locus_tag="SCM56_001597"
     CDS             5616..5960
                     /locus_tag="SCM56_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEG1110378.1"
                     /translation="MNEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            5965..6246
                     /locus_tag="SCM56_001598"
     CDS             5965..6246
                     /locus_tag="SCM56_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110379.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            6349..6663
                     /locus_tag="SCM56_001599"
     CDS             6349..6663
                     /locus_tag="SCM56_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HEG1110380.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            6641..8335
                     /locus_tag="SCM56_001600"
     CDS             6641..8335
                     /locus_tag="SCM56_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEG1110381.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            8355..9533
                     /locus_tag="SCM56_001601"
     CDS             8355..9533
                     /locus_tag="SCM56_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEG1110382.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            9496..10182
                     /locus_tag="SCM56_001602"
     CDS             9496..10182
                     /locus_tag="SCM56_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEG1110383.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            10182..11342
                     /locus_tag="SCM56_001603"
     CDS             10182..11342
                     /locus_tag="SCM56_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEG1110384.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            11352..12227
                     /locus_tag="SCM56_001604"
     CDS             11352..12227
                     /locus_tag="SCM56_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110385.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            12224..12535
                     /locus_tag="SCM56_001605"
     CDS             12224..12535
                     /locus_tag="SCM56_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110386.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            12525..12878
                     /locus_tag="SCM56_001606"
     CDS             12525..12878
                     /locus_tag="SCM56_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEG1110387.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            12868..13269
                     /locus_tag="SCM56_001607"
     CDS             12868..13269
                     /locus_tag="SCM56_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110388.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            13262..13666
                     /locus_tag="SCM56_001608"
     CDS             13262..13666
                     /locus_tag="SCM56_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110389.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            13678..14286
                     /locus_tag="SCM56_001609"
     CDS             13678..14286
                     /locus_tag="SCM56_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEG1110390.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            14306..14668
                     /locus_tag="SCM56_001610"
     CDS             14306..14668
                     /locus_tag="SCM56_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110391.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            14671..14853
                     /locus_tag="SCM56_001611"
     CDS             14671..14853
                     /locus_tag="SCM56_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110392.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            14870..18289
                     /locus_tag="SCM56_001612"
     CDS             14870..18289
                     /locus_tag="SCM56_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEG1110393.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            18340..19077
                     /locus_tag="SCM56_001613"
     CDS             18340..19077
                     /locus_tag="SCM56_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEG1110394.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            19087..21378
                     /locus_tag="SCM56_001614"
     CDS             19087..21378
                     /locus_tag="SCM56_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEG1110395.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            21402..>21988
                     /locus_tag="SCM56_001615"
     CDS             21402..>21988
                     /locus_tag="SCM56_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110396.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSI"
BASE COUNT         7933 a         3245 c         4705 g         6105 t
ORIGIN      
        1 aaataagcct caaagacgaa tcgaatacaa ccgtgagtga tgccaatatc ttagcgaata
       61 tcatcctaga tttcatcttt gaacatcata ttcctttcaa agaaggatat gagattttac
      121 cagcgaatca agagtattac ttctacaaat gcattacaaa aagagtctgc tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgcaaag
      241 aagttgatca tgcagagtac acatttgcag cactctgcag aatccatcac acagagaagc
      301 acaaaatagg tgtgattaat ttcaaaaata aatatcaaat caaagggatc aaattaaacc
      361 agaaaacaat caaaaagtta aatattggag ggtaaaaatg acagaacatc gaagttatta
      421 tgcgattata ccagccaacg taaggtacga caaaagactt aaaccaaata ctaagttgtt
      481 atacggagag ataacggcct tgtgtaatga aagaggcttt tgttgggcag gcaatgagta
      541 ctttgcagat ttatatggtg tgaataaaga gaccatatcg cgatgggtaa gtgatttgat
      601 taagtttgga tacttgaatc gggaaatcat ttacaaagag ggtaccaatc aaataatcaa
      661 taggtaccta cgaattaatc aataccctat tgacgaaaaa cgcaataccc ctattgacga
      721 aaaagtcaaa gataataata catctattaa taatacattt aataatacaa aagaatatat
      781 aagagagtta ccgccttcga aaaaatcgaa ggctaagccc gtccgtcata aatacggaga
      841 gtataaaaat gttcttttgt cagatgagca gatggagaaa ctcaaaacag aattccctaa
      901 tgattaccaa gagcgaatcg aacgactgtc agagtattgt gaatcatctg gtaagactta
      961 taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa gaaaaaagtg aacctaagaa
     1021 cgcaagcagt ggatacaagc gcacaggaag acgagagaag cttcctgaat gggcaatcga
     1081 ccaagaagcc tatctcaaga aaaaagcgct agaacgagct aatagacaat caaaagcacc
     1141 attctaagag gtggaaaaat gaagatcgat tatctagaac taattaatga aatagcgaat
     1201 tataaaaagg gcgaggaatt agacgtcctg agagacgtat atgatcaact cgaagaagct
     1261 ggaattgaag gaattaagaa tgatcgttcg agttggagta agctcagata ctatttcgca
     1321 ctttatatcg atacaacaca attaagaaat ttagcatata ccaaattact atttgttgat
     1381 tgtgttaaag gcttgcaaaa acatcttaat gaacttgagc aggtgtaatc agatggacct
     1441 aaagacattt acagcacaga ttgaactaat gcatcaagaa gctttaagac aaagtgtatc
     1501 gtacgaagac aagtggctca acacgttcca tggcggacgt gagagcgcac ttgatcaagt
     1561 actcaaatta ctgaaaggag aatgtcggga tggataagaa agcggcaatg cagcgaatta
     1621 tcgaattgac ttattcagaa gattggcaaa atgacaaaga agctgcttca gaagtgatga
     1681 ggcttggaag agcgatgtgg gcagacaaga gcaacaagcc aagaccacga aaaatcgcaa
     1741 tttggcacgg tgacaaactt ctagtgatag ggacagctga acagttagca agtctcacag
     1801 gcttgcacga gaaaatcgtg aggaaaagag caaggtgtgg ctacacagac gctaagaaga
     1861 gaacgtttag atacgtggag gaatcatcat gacaacagaa gaagtgattc aaatgcgtat
     1921 tcgaagcctt cagcgtgaga ttgacgatct ggaacgaaca aaggcagtga tggtcaatga
     1981 aacggcgaga aaggcaatcg atttgcacat agagaattta agaagggaaa tccatcgatt
     2041 ggaggaatga gcgtggataa gaaagcagca atgaaaagaa ttgctgaatt aaccaagtca
     2101 gaatcttggc aagaagacaa agaaatagtt gcagaagtcc aaaagctcgg taaatcaatg
     2161 tggactgaaa agcccaaacg gagaacgccg agaaaaattg caatctggca tgatgaccga
     2221 attttagtaa caggtactgc tgaacagtta tctgaaatta ctggattaag caaaaacatt
     2281 atctgggata gagctaggag cttatggatt gattcaaaag gacgacagtt taggtatgtg
     2341 gaggagagat aatggatctc attacacaat acagtgacat catcctcaag aaaatcatga
     2401 tgaagattca gaaagataaa aaatcaaaag aacgagctga attagttaag ttagaaatgg
     2461 ctgaaacagg agcaggagtg cgaagtagca ggcattggaa agcagcagca aacattgaat
     2521 tttattacaa cgaaattcaa aaagggttcg atcagatgcg tgagctggat cggcaaacaa
     2581 attggagcaa gaaacttcat caagatcgtt tcaaatttgt agagaagtat aaagaaatat
     2641 tagaagagta tttgaggagg acagcaaatg ataaaaaaac tcgttcaatt cagcatggat
     2701 ttatatgata tcgaatcagg agcaacacta tctgtggaat cggaccatct aatcataaat
     2761 tttggtggaa agcgccagat tattttgtgg gtagttgatg atgtactgtt tccagaaatt
     2821 gttcatgatt tcgaagaatc aaaagcggtt gagtttgaaa tagtgaaaaa agtaatggaa
     2881 ttgattgaaa aatacgagga ggacagcgaa tgataccgaa gtttagagcg tgggataaac
     2941 gaaagaacgt aatgagagat gtagccgtct tgcattttac taaaaacggc aaaacaaact
     3001 ttattgaata ttggataaat cctaccgaat tgaaatcata tcacgtgcga aacatcgacc
     3061 tcatgcaatc cacaggaatg aaagataaga atggtgtgga tatatttgaa ggggatatag
     3121 tattagtcag cgtgcgaaat ggcttcgatt acttagataa taaagtctgt attgtcaaaa
     3181 attcaataga ttattccgga ttagtttgtg ccactgttga tgaagactta gagtatcaaa
     3241 tttttaacac agagctgttt gaagaataca cgtacgaagt caccggaaat atatacgaga
     3301 atagcgagtt attggaggaa cagcgatgaa taaacaggaa gcaatcaaaa agttagaaag
     3361 tattaaagcg ataggaaatg atgcaatagc tgcttgctat aacgagagta taaattcagg
     3421 tattacgtta atgaaaaaaa tagacgaacc gcagaaacca gttattccac aactcgtggc
     3481 cggttggctt gagaaatcta cggacccttt tacaaaagct gaaaaaatag cgtatttaat
     3541 caaatctaaa gatggtgatt catattattt ctgtgattgg tttgtacgag atggcatagt
     3601 gacgcaagag caaggagaag aattactcgc ttgggcaaca agacaatcat atgaaacact
     3661 attgagccta tacaacggct acgaggttga gaaagagccg ttatgggcaa taaagaatgc
     3721 cgatggaaac tatcttacta aatgtgcttt atggggaaaa gatggagtaa attatagttt
     3781 tgaatgcaat ccatctcatc gattgctttt cactgataaa gcaacagcgg atgctgcagc
     3841 attgttggtg aatggaacag tggaagaggt ggtagaaaga tgaaactaaa agacggattt
     3901 tacgctagca gtcacggtat cggcggttta atgctagata tgccgacaaa gaaccctaaa
     3961 acacgcaaga aatcaaaatt caaagtcggt gacatggtcc gctgtgaagc agaagagttc
     4021 gtttatccgt tcagaggata cgttaaaaag atactgtcaa actcagcaat cattcgtatt
     4081 gaaaatacaa tgaagtgtga tcgttcgact gcaaaacaca agcattattt agctgtagcg
     4141 agattagttg acatggaagt aatcaagagc aaataaaaaa agccggatcg ctccgactga
     4201 ttcaataaat ccaacacatt tattatatca cataaaggag cggtttgact tgatgcaatt
     4261 gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc tgaagaactt
     4321 tcggcgtttg gagcggatgg caggtcgctc tttgatagat attaagtcgc cgattattac
     4381 ggatatgccg aaggcaccaa agcacggcaa taaggcagaa gacgcgatca ttcagatgat
     4441 ggatatagaa gcggagagag atgcgattct agcggctttg atggctctta gtctaattag
     4501 tcgccagata ctctactaca gcttctgtgt gccagatagc ttctcaaact acaggattag
     4561 ccgtgaagtg ggttattcag aaagaagtat acaacggatg aagtcggaag ctctaataga
     4621 gtttgcagaa gcatataaac acggaagaat aattgcttat aaataatttg gcggtttttt
     4681 ggcggaatga tggcggtttt tagctattta ccagtgatat tatggtagtg tcgaaagatt
     4741 agtgataggt ctaagacaaa ataaaatgta agggaggaaa tctccctcat cgtttaatta
     4801 agcttcgata gacagcagca aataaactaa aggatgtggg ggtcagctcc tacagttagt
     4861 tcatgtgttg ctgtctatta attgcaactc tttcggtttt attgagtatt tactcaactt
     4921 aaaaaaactg ttgtttgtag atgtaagctg ttcctatttg tacatagtaa aaaatgatat
     4981 aataaaaata ttacgaaagg ggtgagagtg catggaagac aaaatgtatt taagacaaaa
     5041 agttttcaca aaaaaagata ttgaaaatca aattaatgga tttatacaga aattagaaaa
     5101 tgcaaattct tatttgataa ataaggaact aaataaagct tatgaccagt ggaaagaagt
     5161 atatgatgag ttgaaattga ttcaaaatga aacaaaatta gtacgaacgg agaaaaagaa
     5221 tgaaaatagt ttcttttttg atggatatgc tatattgatg ttggaaactg tagcaaaaca
     5281 aaatataaaa gctcccaaaa aagaactatc tgacaatatt gataacgcat tagccgagtt
     5341 aaggtattac gttatgcaaa taaaagatgt taggatcact cgttgagtgg tctttttatt
     5401 ttgcacaaag gaggtaacaa caatgtatag accacaatac ttagaacaga agtatgaagt
     5461 aatcactgtg caaaatggta acggagagat agtacgaaag tatagaagac caataaagag
     5521 cgatacatat aaacgaaagg aaagcaatga agttattcca ttgtatggca aaagaatagc
     5581 taagcattaa ataagattgt gaaaggagac gggacatgaa cgaggaattc tatagatggc
     5641 tattacagtt gataagagaa gatcgtttgg ttaagttcta tcagtctcct aaatggcgca
     5701 ggcttagaga gaaagcgatg aaacgagatc actatgaatg ccaagagtgt agaagactag
     5761 gtaagtatca tagagtagag aacgttcatc atataaagga agtcaaggat agacctgact
     5821 tagctttaga tttagataat cttatttgtt tatgtgttga acatcataat gaagttcatg
     5881 gcagatatct tacagcattg gataaacaag agaagaagat agaaagcttt gctaacttcg
     5941 atgcaagtga aaggtggtaa gtgcatgatc atcaatgata atggcagaga gtatgataca
     6001 gaaaagattg aagagtattc atcttatact cagggattaa ttaaacgttt gatatacgtt
     6061 cgctatgtag gtattaggga tctgttatca gataactgtt gtagtaaata caaagtgaat
     6121 caagtaagag aagcgttgaa taaagataat aacgtcgaaa gaataaaaaa tgtttttgga
     6181 tatagtattg aagagattaa ttattacatt gacttcgctg aagctttcat tccgatggtg
     6241 agataacccc cccttaaaat aaatcgcaaa ttttttgggg gtgatgaaac ggagggggct
     6301 gtcaggaaaa gagatttttt cgaactttat catgaaagga gggctaaaat gtttaaaaac
     6361 gaattgtctc aaaatcgcta cagagaaaaa ttacgccgct ctttaataag ccaattggaa
     6421 agtcagaaaa caaatattga gccattctta gataatgttg atcgttatat cagtttatgg
     6481 gaaacggcga tatcactgga agaagatata tccgagaacg gcattagact ggagaatggt
     6541 aaaaagaatg aatcagtagc gttgcttgtt tctgtcaaca aacaaatggg attgatgttg
     6601 gataaacttg ccattactcc tgaattggta ggtgaagcaa atgaatcaat tcctgagtta
     6661 taagcatatt gaaaattggt tcaaagctat agaagaaggc actatcaagg tatgcaaaga
     6721 gcaattattg ctaaaaaatt atctagaaga aagagtcttt actagagaag atatttactt
     6781 cgataagcag atggtagagg attcaatcaa tataccagca caatactttc cattcgaatt
     6841 aattccgtgg gaaaaatttc tacaatgttt tatttatggt gttcgatgga aaaaagataa
     6901 aacactagtg ttcaatagat atctttcatt aatgggacgt ggtaatggta aaactggttt
     6961 tgcttcttgg aacaacttct ttctactaac cgctaaacac ggtattaaaa attatgatat
     7021 tgatatctat gccaataatg aaagccaagc aaagactagt tttgatgatg tatttaaagt
     7081 aattaaagat catcctgatt tagataaaaa agtatttaaa gctacgaagg aagttattca
     7141 aaatatcgct acaaacagca aacttcgtta taacacggca aatgctagaa caaaagatgg
     7201 gaagcgacca ggtgcaaacc gctttgatga aattcacgaa aatgaagatt attcaatgat
     7261 aaatgtggct acttctggtg gtggtaaaat tcgagattat agagaatttt atgatacaac
     7321 taatggtcat gttcgtggtg gtccgcttga tgacattata gaagaatcaa aaatgattct
     7381 ttctggagaa cttggaattg acaaggatgg agcagaattt tctagtttgt ttccatttat
     7441 ttgtcgcttg gataacgata atgaagttga tgatcccgac atgtgggaaa aagcttgtcc
     7501 aactattaat tacaatgcaa atttaaaacg gaaaatgttt caagaatact ctcaaatgca
     7561 acgtaatgct ggtttaagac ttacgttcat gaccaaacga atgaacagac ctatggaaga
     7621 tacacgattt gctgttgctt catatgatga tgttctgcat acgaaagaaa aagaatttcc
     7681 tgaaaaaatg gatgaagtga taggaacagt cgattttgct gatagacgag attttgccag
     7741 cgttgggttg ctaggaaaat acgataaaga tgtttatttt acacaacata cttttatcca
     7801 cgaatcagcc cttcgattac aaaacatcaa acgagaggtt atagatattt ctatagatca
     7861 aggaaaatca cagatcgttc atggaaaaaa tatagaagct gattatattg taggttggtt
     7921 tcttgaaatg agtaataaat attatattaa aaaaatcgct atggatatgt accgtgcaaa
     7981 aatattgaag cccgctttag aagaagcagg ttttactgtg gaaattgttc gaagcggatc
     8041 tgttacacat ggtatgttaa aagatctggt tgatgacctt tttattaatc aacgtttatt
     8101 ttttggtgac gatgcgatta tgcgttggta ttgcatgaat gtatatgaag agcatatttc
     8161 taatggaaat atacgctatg aaaaaataga acctgaaact agaaaaacgg atggcttttt
     8221 ttcattcctt catggtttga attttttaga tgatatttat gattctgctc ctgtaacagt
     8281 cacaaatagc tcagtagaaa atacaggaac tggatttact cctctagtat tctaacttga
     8341 aaggaggtga gaaagtgggg atttttcaaa aggcggtagg atacttcaca aaaaaagcaa
     8401 cggttccttt agaagaatac ttttgtaaat tgcaagttga ttttgtgtat cgaaaatttg
     8461 cgattgaaac ttgtattgat ttgattgcaa atgcgatgag taaagcggaa ttcaagtcat
     8521 atgaagatgg aaaaaataaa aagaatgatc tttactatag gctgaatgta gctcctaata
     8581 agaaaaataa tgcaacagaa tttagaaaaa aactgatcag gagattaata ttctacaatg
     8641 aagtattgat cgtttctccg tctaataatt ctagcgaaat atttattgcg gatagttggg
     8701 atgtcacaga atatgcattg aaagatgatg tgttttctca agtgcaaatt aacaacatag
     8761 tccttgatag agaatttcta gaaagtgatg ttatctatat aaaatacgca gatcaacaaa
     8821 ttaggcaact agtcgatgcg tattatcaag cgtatgggaa actcatttct agtgccatga
     8881 atgtttacaa gcgctctaac gctcgtagat acgtactgaa agggaattta ttccgaccgc
     8941 aagacaatac aacacaagat caaatcaata aaatgatgac atcacaattt aaggctttta
     9001 tggaagctga taatgcaggt gcggtatttc aattacaaaa tgagtacaca ttagaagatt
     9061 tcagcggaaa cttccaaagc aattcaagag atataaaaaa cttaatagac gacatctttg
     9121 agatgacagc agcagcgttt cacgttccga aaaacctact aaagggagac atgagtgggt
     9181 tatcggatca agtggacgct tttttaatgt tcgaaatcat accgattgct gaacttattc
     9241 aggatgcgtt taacgctagt ctctatgaag tagaagaata cttgtcaggg aattttgtac
     9301 gtgtggatac aactatgatc aagattacta gcttcaaaga tttggttgac gctattgatg
     9361 taggcattag aaatggagta tttacaatca acgaaggaag agaacgcgtt ggaaatgatc
     9421 gctctgataa ggcgatggca gatgaaatat ttataactaa aaacaatcaa caagtatcga
     9481 aaggaggtga ggcgaatgac gacaatgaaa acatttctag cagtaaagaa tgaaggcgca
     9541 gtaccgcaaa tttttattca gggatttatt ggttctagtt ggttctttga agggaatact
     9601 gacaagggaa tcaaaaatat tttggatagt ctaggtgatc aagaagaaat tgaagtagta
     9661 attaattcaa acggtggaga cgtatttcaa gggattgcta ttgggaactt acttaagtca
     9721 aataaagcaa aagttaacgt tgtgattaac ggcttagccg ctagtgctgc ttcaattatc
     9781 gcaatggctg gcgatactat aaaaatctac aacaatgcac aattgatgat tcaccgcgct
     9841 tccacatacg gagaaggaaa tgtcgatgac ttccgtacga ttgctgacca actggaatca
     9901 attgataaat cggtaaaggc ttcatataaa acacgattca atggcacaga tgaagcattg
     9961 caagaacttc ttgaaaaaga atcgtttatg gatgcagaaa cagctttgag ttatggattg
    10021 gtcgatgaaa ttatcgatgc agaaaatagc tcaggtactg aagctaaaaa agaacaaagc
    10081 gttgaagaaa ttttgaatga cgttaaagaa aaaagagcag aaaaaattgc tgcatttaca
    10141 gcagcattaa ataaaacatt tggacaagga gatgtaaaat aatgacagtt aaaaatttaa
    10201 aaggtgtaac agctgcaagc gaccaattga tgaaagcttt taaagatggt aacgaagaat
    10261 cttttagcgc agctatggta agcttatcta aggaaattca ggataaaatt ttagaagaag
    10321 caacagcaaa aaatcaagat caattagtat taatgaaccg tggtcagcgt gtattaacta
    10381 cacaagaaac aaaattctat aacgaagtag tgaaaaacga aggttttgca ggggtcgaag
    10441 aattagtgcc agctactgta tttgaacgtg tatttgaaga tttagaacaa tctcatccac
    10501 tattgcaaaa aattactttt gttaacacaa ctggtgtaac agaatggatt gtgtcacgtg
    10561 gagtcaatcc agcatggtgg ggtaaacttt gcgaagctgt taaaaaagtt ttagataatg
    10621 gctttgatgt aattaacatg aagcagttca agctatcagg ttatattcct gtatgtaagg
    10681 caatgcttga tttaggtcca gtatggttag atcgttatgt ccgtactgtt ttagtagaat
    10741 cattgagaat tgcattagaa caagcaattg ttgatggtac tggtaaagat atgccagtcg
    10801 gaatgatgcg tgacatgagc aaacaaacta gcggagaata tgctgaaaaa aaagcagaac
    10861 ctattacagc tttagatgct gtaactatgg gccgtttgat ggcgcgacta tcaaaattca
    10921 atatcgaagg tgtgaatgat ccgatttatc gtaatgtgaa tccttctgat gtggtcctaa
    10981 ttgtgaatcc aactgattac tggtctaaag tattcccagc taagactgta ctaactgcta
    11041 atggagaata cgtacaagta ttgccagtac cagtttcaga tttgcagtca acggctgtgc
    11101 cagaaggaaa agcagttatt ggggtagcct cagattactt catgggtgta ggatctacac
    11161 taaaaattga agcttcagat gaataccatt ttgttgaaga cgaacgcatt tatctagcta
    11221 aacaatatgc aaacggtcaa cctaaacgta acgatagttt cattgtatta gatattagcg
    11281 ctttgggaac tactactaca actacaaaac caacaaccac aacaactaca acacaagcgt
    11341 aggtgatcag aatgaagtat attctttgtc agccggcaat caatcggttt aaatgggagc
    11401 ttgaagtttg tttaactaat ctgaagaaac taggaatcaa agatatcgta ttgcttttca
    11461 gcagacacga tgatcagatt cctatttttt ttgagaagga atatggtgtt gaagttcatg
    11521 tgtacgatga tctgcgggac gacaaagagt atattccttc gattaaacca tatttatggt
    11581 ggaaatattt agaagaagat cattcgcgtg aggacgaccg atatttctat atcgattcgg
    11641 atgtcatttt caataaaaga attaatttgc gcaaattgcc ttctaaagat gatgtttggt
    11701 attgtagcga ctgctgtagt tatctaagtc ttgattatat tagaagctgt gaaaacggag
    11761 aaaatattct aaaagatatg gcaaacattg taaatgttac agtagaatct ttggaaacta
    11821 taaacactaa ttcaggaggc gcacagtggg ttattaaccg tcctaaagcg aattattgga
    11881 aaaaggttta tctggattct aatcggctat atcgctacct tagagggcaa aaaacaaata
    11941 tacaaatttg gacagccgag atgtgggcac ggctttggaa catgatgtat ttcaatattg
    12001 gtcctaaagt tcacgaggaa ttagactttt gttttgctac tgatccaata gaaaaagtta
    12061 aagaagtaaa aatcttacac aatgctggag taacaacaaa cgatgaagat ttatttttca
    12121 aagggagata cgtgacttct acgccttttg atgaagattt atcatttgta aacaagaaaa
    12181 aatgctctta cgcatatgtt aaagcaatta aggcggtggt tagatgacgc ctgaacaagt
    12241 gactgaagaa ttgctaatag ctgtgaagga taatatttac gttacctgga acgaagaaga
    12301 tgagtcaatt aaaaagatga tagctaaaaa tgctgtttat cttcaaagta aagtgagtac
    12361 aacactttct ttttctcctg aaagcttaga atacggattg ctaatcgaaa gatgtagata
    12421 cgactggaat cgtgctttag atgagtttga acaaaatttc gctagtgagt tattaggttt
    12481 cattcaacat tatgcgctac aagaatatat tgcaggtgat gtgaatggcg aataatcgta
    12541 gactcgaaga aacattcaac gatggttggt taaagatttt gacgcaaact accaaaagaa
    12601 atgaactagg aaaaaagatt ggtgtagaag atacagaaat cacttcttta aaatttagaa
    12661 atctttccat gagagatagt gatataacag ctatggatgc gatgggatcg aaattaacta
    12721 agaaagtaaa gactccattt catccaatcg ccaagaaatt taataaagat caatatttta
    12781 tcgtaatcga tagtatgcgt tacaacgtta tctatgccga ttacgataat ttttatatct
    12841 atttttatct tgaaagtgtg ggtgaatatg gtgattgata attctaaaga aaaagaacgt
    12901 ttaaataagc aaatttctgc tatcaaaact tccttagaag agcattttgg cctcaaactc
    12961 tttcaagact ccgtaggcga ggatgagcta cctgatgatt ttaattactt cattctcgaa
    13021 acaggagaaa tagaaatgat cactgagcca aaatatagcg tgggtcaaaa tctatatcta
    13081 actttctatt cagaaaatag agaagattta acaggagact cactagatat tatttcattg
    13141 attcaaaatc gttcgattcg ttttcagaga atggatccca atcatttaaa actagagaac
    13201 caagatcgct atatcgatca attggtattt acgtttagac gattattgaa gagtgattgt
    13261 catggctaaa aatagttggg agctaaaaat aaatggacat gatgaacttc ttgtgcggat
    13321 ggaacgctat tcaagcgaga gcgaacgact gattaacgaa gcattgaaat caaaaggttc
    13381 ggctattgca gtggatagga ttacagaaaa aattcctgtt tctgaagcag atttaagaag
    13441 agggcaccaa cacgcaaaaa atagtcgtcc acttaagact caatacatta atttgggttt
    13501 catcattaga cctacaagaa aatttgagta tttaaaatat cctgatttgg ggataggtac
    13561 ttctaaaaga aatcagccgg acgaatttat gagaagagga ttaggtcttg cacttgatcc
    13621 aattacagaa cttctgattc gtcaattcga taaattaaat aaataggggg aacaacaatg
    13681 gctaaaacaa aaactgtagt aacaacgttc gataacgtaa gtatcaaacg aattgctttt
    13741 aattttaaga acgcaggaaa tgcaatcgca acgaattgta atggacaatt agatggcgaa
    13801 acagaaatgc aaacggtggt taaaaaatgt ggagcgacag aagtaaaatc aaaatctaaa
    13861 ccaatcaata tgacggtaac aattactgca catgtaccga tggaagttta tcgacgtttc
    13921 aatgggttga aacaagatgg acgtattaaa ccaggcattt actcttacgg tcctgattcc
    13981 gtaggcgaag atttctcact tgctgcagag atcgtggatg acttcgaaga aaatagaaag
    14041 ttagttggta tgttagcatg cacttcgaat acaggattaa cattctctat tgaaaatggt
    14101 gcggatgaag tagctgcgtt agaactagaa acaaaagtta tgcaagatga atttggtaaa
    14161 ttttatcatg aagcaattgt tgcagaactt gaagaagact taacagatca atggatgacg
    14221 aatctatctg ctgatgtgat taaaaaaact tcagttgtga caactacggc cactcaatca
    14281 cagtaaaaaa aacggaggta gcgaaatgaa cgaagattac tcaaaaattg aactaaacga
    14341 tggaacaatt ttgaatttag aacctaaact gaatatcaag aaattattga tgatcaatag
    14401 agattttaac acagacgagt ttgcaaaaat gactgtggga aaaggatcca tggatatttc
    14461 tgttattcaa ggtgcaaagg ctgtgtatat tgcttaccgc caagcgaaca tgactgatta
    14521 tatttcattc gatgaattta tcgataaatg ggattttgat atggctactg ccagctatat
    14581 ttatcaattg atgatgttca aacaagcacg cgatgcttat caaaaagaat ttgaaaaagc
    14641 aaataaggaa aaaaagcttc aaaagtaaaa atgccaaagc tcttagttga aacgtgggtc
    14701 gatgtctatt cgatgttgac cgacgttttt tctatgcctt cagatttggt tttaagcgat
    14761 atctgtttag atgacatttt gcaaatggct tacaacaaga gtgcttatga aggatggaaa
    14821 aactatgcaa taaaccaatc tcagaaaaac taaagaaagg aggtaaaaaa tggctaaaaa
    14881 gagaacagaa gcagaagtaa ctttcatagc taacgatgac ggattgaaat ctacgttaaa
    14941 agaaatcagt gctgaattaa ctaaaaatag agcagaatta aaactagaac aagctcaatt
    15001 acaacagact ggttctgaat cagacaagtt aggaagtaaa ttatcttctt tagaaaagca
    15061 gtatgaatta caaagccaaa aagttgaagt aactagccaa cgtttagcca atgccaaaaa
    15121 atattatgga gaaaattcca ccgaagttca gaaacttgag agagaactga ttaaccaaca
    15181 aacagcgcaa caacgtttgt caaacgaaat tgataaaacg agtaatacac tagctcaagc
    15241 aaaaggcgaa atacagacgt acgagtctac aatgcaacag ttggatagtg aacaaaaaaa
    15301 tgttcaagct agtgcttctc tgattgaatc cgaatacaaa aaatggcaag taactgctgg
    15361 tcaatcagct tctgaatccg agaaattagc gaaagcccaa gaatatgttt ctcaacaatc
    15421 tgaaaatgca gagaaaacga tagatatcct gagacgacag ttagaagcta cacagtctga
    15481 gtttggcgct acatccacag aagcaatgca gatggaggcg aagcttaatg atgctgaacg
    15541 tgaatttgaa gagttaggac aagctgctaa aaatgtagat acaactaact tggacgatat
    15601 cggaagcaaa atagatatga ataatttaat ggaagcttct gacgttttaa gcgacattgg
    15661 cgataagctt acagaattag ggaaacaagc agtggactct gctaacagtg taggtagttc
    15721 ccagagtaaa atacaagcta attttggttt gactaaacaa gaggctgaag aattaacgaa
    15781 tgtagccaga gacatttatt ataaaggttt tggagaatcg ttagatcagt ccacagatgc
    15841 attgattttg gtaaagcgta atttaggcga tttaaataat caagatttac aaaatattac
    15901 ggaacaagct atggtcctag aaaacaccat gggcgctgat atggatgaaa cgttacgtgg
    15961 tgtaaatggc ttaatggtca acttcggatt gagcgctcaa gatgcaatgg atttaatggt
    16021 ttcgggtact caaaacggtt tagataaaac gcacgaatta ggcgacaata tggcagaata
    16081 tagccaatta tggagtcaaa tgggatattc agctgatgaa acgttcggaa tgcttcaaaa
    16141 tggtttagat gcgggtgctt ataaccttga taaagtcaat gacttagtta aggaaatggg
    16201 aatatcgtta acagatggtc gatttgagca aaacatggat atgtttagtg aaagtactag
    16261 aaaagctttt gaagagtgga aaaatggtgg aggaacacaa aaagacgtta ttaattccat
    16321 gattcaagat tttagcaata tggatggtca atacgaccaa ttaaataaag cttctacaat
    16381 ttggtctgca cttggcgaag ataacgcgat gaaagttgtc caatctttga ctgatgttaa
    16441 ccatacattt gatgatgtta gtggatctgc acaaaaaatg aatgaagatt ctactactcc
    16501 gttacaagaa ttaaacggaa aaatagctga attaaaggat tcattagctc ctataggcaa
    16561 cacaatcata gatgcactcg aaccagtaat tgattttcta ggaaagatgg ctgatgcgtt
    16621 taataatctt ccacaaccag tacaggatta tgccgtagcg attggcggat tgactgctgc
    16681 atttacttta ttaatgccaa taatagttgg cttcatggct ctaggtggtc ctactacatt
    16741 aataatagga gcagttatta ctgctattgc tggagttata gcaattataa aaaactgggg
    16801 cgcaattact gactggttta agggaatatg gagtaaattc actgattggt tgggtggtac
    16861 ttgggaaagc ataaaagaag gtgcctcatc agtttgggat ggagtcaaag aaacctggtc
    16921 tggatttgta gattgggttc aagaaatttg gcaaggagtt tctgattggt ttggagagtt
    16981 atggagcgga ttagttgaag gagcttccaa catctggcaa ggagtccaag agacttggca
    17041 agcattcgtt gattgggttt caaatatttg gaacggagtc aaagaagtat ggtcgattat
    17101 ttgggcagac attgtaggaa ttgttcaaat accatggaca ttaataacgt cattgattca
    17161 agctggtatt aatattatcg tgggtatctt tgatgtagct ggacagttat taggcgcagc
    17221 ttggcaagct gtttggacac ctatttctga tttccttaaa aatacttggg atactatgac
    17281 acaatggata agcatcgctt ggaatggaat tgtaactaca ttccatacta tatttgatcc
    17341 agtagtggca tggtggaatg gtatatggac atctattagt actacggctt caaatatttg
    17401 gaactcaatt agtgcaacag cttctagtat ttggaatagt atcaagaata caatcactag
    17461 cttggtacaa gcagctgcta cagtaattca aaatatttgg tcaactgtat ctagttggtt
    17521 aggtggaatt tggaattcaa tcagctctac agcatcaaat atctggagta gcgtgactag
    17581 tagtataagc aatgctataa acgcagctaa aagtgccatt caaagtgttt ggaatagtat
    17641 atcttcgtgg atcagcggaa tttggaacgg tatcaaaaac actgctttga atctttggaa
    17701 tggaattaca agcactatta gctctaaagt aaacgatgga aaaaatgcaa tttcaagcgg
    17761 ttggtccaat ctaacaggta ttgtttccga catattcaat aatgttaaaa gcacgatagc
    17821 taatatatgg gaaggtatca aaaagactgt tagcgctccg attgattgga ttagagataa
    17881 aatcagtagt gtctttgata atttgaatat ttctatacca catattccgt taccacattt
    17941 taaattgagc ggagaattca atccattgaa ggggaaaatc ccaacattgg gtgttgattg
    18001 gtatgcgaaa ggtagtgtgt ttaattctcc aaatattatc ggtgtaggtg aatcaggacc
    18061 tgaagcagtt ttacctttga aaagatctgt gctgcaagaa attggtgatc gtatcttgag
    18121 tagcacctca gtttcatcta ggacacaagc ggttcaacct gtgaacaatt acgaattcaa
    18181 tttcacaatt gatggtaacg cagatgaagt cactatgaag caaacaactc aacaaatcat
    18241 tgatagcatt acaaaagttc aaaatgataa tgcttcggca tggcgttaaa caggagagta
    18301 tttctcctgt ttttttagta ttaaaaagga tgtgaaaaaa tgactgattg tatacattct
    18361 ataatcgatg gatttcctga ttatttgcat aaattggctt tagctgaaag accaaccata
    18421 ccttctccaa aaagacagag agttgaaact tctgttttag gaaggttagg tggcttagtg
    18481 caagattact cgtttgaaga catgtcgttt acattgcact ataactattt agaggatgtg
    18541 gaagaccatc aagcgttcaa gcaatcgttt tatatcatgc gtcattggtt aaactatgca
    18601 aagaaattag aattctctga cgatcccaac gtctattacg ttatccagac tatcgatatt
    18661 ggggatgcag aaaacgatat tgttgaatgg ggagagttcg atgtaaatat tactgcgaaa
    18721 ccattcgcaa gagttcaaga agatgtacct ataaccgtag ataaaccaca gtcatttaac
    18781 ttgctgaata atagtttaga agaaagtttt ccaaagatta tcatcactcc ttcagctact
    18841 tcatgccagt tcatcttaaa tgattatgtg tttagtttcg aaggcttagt agcaggaact
    18901 gatgtagtca ttgatagtga tttgatgctt tgctacgaag agcaatcgga cggagatatt
    18961 ttagatcggt ccaacaaaat gaagaccatg caatatccga cattgcaagt ggatatcaat
    19021 cattttaatt gcactggttt gagcaaaata caaatttatc gtaacgggtt aaggtaggtg
    19081 aaatagatga tcgataattt aataactatt tacgataaaa atgacgcgaa taatttagct
    19141 gaacatttat atgatacgca aggtttaggc gctttgtcag actggttaac agctactgtt
    19201 agcaataaac taaacggagc cgagatattt cagggtactt atccaataag cggaactaat
    19261 gcagatttga ttgtagaagg acgtattatt cagtgttatg tagatgaaaa tcgagcaaaa
    19321 cagcgtctac ggatttatta tgcaaaaact tctgtaatag gcaatacgat agaagtaaaa
    19381 gctgaaccta ttttcaatga tataagaaaa tcggtgttga ataaatatga cagcggaaca
    19441 gaaaaaatca ctgctactca ggcatggcaa aacgcaaaag ctttagcgaa accagctatc
    19501 ccttcgcagt tttctttctc gtcattagta gatacgcttg ctaatgtgaa gatagaaaaa
    19561 gcgaattttt tagaattctt tggtggaaaa gagggatcta ttctagatcg atttcatggg
    19621 gaatttctta aagataataa cacattacat catgaaaaaa ggctaggtac ggatcataaa
    19681 atcaaagcga tttatactaa aaatttaact ggtcttgact tagagataga tgctcaaagc
    19741 gttttagttg gagtttatcc attcattagc agttcttcag aaggagaaga cgagatcact
    19801 ctaccagaag aagtcatttt cacggattac gtggatgatt atcctgctgg atatgtttct
    19861 tttgttgatt ttaaagacaa agcgactgat gtagccacat taagggaagc tgctaaagac
    19921 tggttgaaaa caaacataga taaacaaaaa ccacaagtga gtggttcgat tgaattagta
    19981 ccattgaggc atcaaagagg ctatgaaaaa tttgttgatc tagaaaaagt ttcgatgggt
    20041 gacggagtag atgtgtatca tccacagtta aaagtgaata tgtcagcgag aattgtggaa
    20101 tatacgttta atgttctaac caattcatac gataaattag ttgtaggaaa cgtcaaaaca
    20161 aacttcttag aaaacacaga gaataatgta agcaatttga ttaatgatgc cattgatcaa
    20221 ttgaaaaatg gtggcgaaat cagtgattta atcaatgata ttgtagatca tcaaactgat
    20281 atgattactg gtcaaaatgg tggttatgtt ttattagatc ctaaagaagc gcctagtcgt
    20341 attttgatta tggacacacc agataagaat accgcaagga atgttttaca aatcaacaat
    20401 gctggtattg gtttttctaa aactggcatt aatggaacat atgaaaccgc atggacatta
    20461 gatggcggat tcaatgcctc gtttattacg gctggtgaaa tagtaggaat tactattaga
    20521 ggtactacat taattagtga tggcactgat tatagaacaa gtattgctaa tggcaaaatg
    20581 acttggtact caaaaaaagt taacaaagat attatggagc tagaagcacg tgattatgta
    20641 agtgctgatg ccggtattgt atcatacacc atgaaaactg gtggtggttt catgattaga
    20701 aatccacagg gtaacttggt ttttagtacg tgggataatg gtaataacag accgtttcta
    20761 tcttttggtg cgcccaattt caggtatagc aatgctagtt atgtaacttc tggcgacggt
    20821 agttctttaa gcattaatgg tagtgcgggt aactcatggg aatttaaggt agctggtagg
    20881 actatgaaat ttactagtga tggtatgcta acgttgccag gttgtttttt tggttcatgg
    20941 gaagatggga aacttgctag gtttgaacaa tcaacggtac aagtatataa agattttact
    21001 gttagaggta ctaaaaactc aaccgtacca acagaacatt atggacaacg actattaaac
    21061 gcttatgaaa ctccagaata ttatttcgct gattatgggg aagccgttac aggtgatgat
    21121 ggtaaagttc gtgttgatat tgaccccatg tttgctgaga cagtaaatct aagtcgatat
    21181 atgacacatg tgacacctac agaactagtt ttgtgtgctg ttactcatga agatattgac
    21241 catttcatca ttgaaactag taagccaaac gtattagtta gatggaattt agtggcacac
    21301 cgtctagggt atgaagatat tagattaaaa gaggatacag catatgatag cacagtgctt
    21361 gaccaaaaac gtttttaaaa cgaagacaag gaggtatata aatggctagc agtttatata
    21421 atttggctct agatttcagc aaagaattaa actacaccaa agctattatg gctcgtcaag
    21481 gtgataaagg gattacagtg actgttaaac catttttaaa tggcttgcag atggatacga
    21541 gtggcggaac atttacttta aaaggaacaa cgccatctaa ccgttacgta gatagtgtgg
    21601 caactagtgt aactagtgaa gaagtcacgt tttctcttga tggcacattt atgagtgaag
    21661 caggatatta taaacactgc tacgtagaat atagaaaaga caatcaaatt ttaacaacgc
    21721 aagatatcat ttttttctca ctaggagtgt ctgacatttc gcaaggccaa gccgatgaat
    21781 acgtttcgca attggaagag ttgattcaaa agtataacga aacttttgat gcttttatgg
    21841 ctgaaatcga aggtagagtg gacagcttaa ataaacagat tactgattta actggtcaag
    21901 ctaaaacact acaagacaag ttagacgctc tgaaagaaga aatttctaaa ttaggtaact
    21961 tgcaggtgat gtacagtaac agcatcga
//
LOCUS       DARBDV010000046        21911 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000046 DARBDV010000000
VERSION     DARBDV010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.046"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="SCM56_001616"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="SCM56_001616"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEG1110397.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="SCM56_001617"
     CDS             complement(1172..1867)
                     /locus_tag="SCM56_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEG1110398.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="SCM56_001618"
     CDS             complement(1880..2596)
                     /locus_tag="SCM56_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEG1110399.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="SCM56_001619"
     CDS             2926..3534
                     /locus_tag="SCM56_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEG1110400.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="SCM56_001620"
     CDS             3859..4182
                     /locus_tag="SCM56_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEG1110401.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="SCM56_001621"
     CDS             4364..5734
                     /locus_tag="SCM56_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEG1110402.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="SCM56_001622"
     CDS             complement(5864..7165)
                     /locus_tag="SCM56_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEG1110403.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9477)
                     /locus_tag="SCM56_001623"
     CDS             complement(7519..9477)
                     /locus_tag="SCM56_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEG1110404.1"
                     /translation="MTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="SCM56_001624"
     CDS             complement(9654..9920)
                     /locus_tag="SCM56_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEG1110405.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="SCM56_001625"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="SCM56_001625"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEG1110406.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="SCM56_001626"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="SCM56_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEG1110407.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /locus_tag="SCM56_001627"
     CDS             13264..13827
                     /locus_tag="SCM56_001627"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEG1110408.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="SCM56_001628"
     CDS             complement(14080..14601)
                     /locus_tag="SCM56_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEG1110409.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="SCM56_001629"
     CDS             complement(14604..15446)
                     /locus_tag="SCM56_001629"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEG1110410.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="SCM56_001630"
     CDS             complement(15439..16164)
                     /locus_tag="SCM56_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HEG1110411.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="SCM56_001631"
     CDS             16340..16636
                     /locus_tag="SCM56_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110412.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17204..18796
                     /locus_tag="SCM56_001632"
     CDS             17204..18796
                     /locus_tag="SCM56_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEG1110413.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="SCM56_001633"
     CDS             18809..19549
                     /locus_tag="SCM56_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEG1110414.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="SCM56_001634"
     CDS             19717..21066
                     /locus_tag="SCM56_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1110415.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="SCM56_001635"
     CDS             complement(21194..21784)
                     /locus_tag="SCM56_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEG1110416.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
BASE COUNT         6294 a         4462 c         4158 g         6997 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caataactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aaccatcgtt ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt
    21901 acttttaaaa a
//
LOCUS       DARBDV010000047        21867 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000047 DARBDV010000000
VERSION     DARBDV010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.047"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(205..1104)
                     /locus_tag="SCM56_001636"
     CDS             complement(205..1104)
                     /locus_tag="SCM56_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HEG1110417.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(1114..1470)
                     /locus_tag="SCM56_001637"
     CDS             complement(1114..1470)
                     /locus_tag="SCM56_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEG1110418.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1825..2121
                     /locus_tag="SCM56_001638"
     CDS             1825..2121
                     /locus_tag="SCM56_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HEG1110419.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(2765..3718)
                     /locus_tag="SCM56_001639"
     CDS             complement(2765..3718)
                     /locus_tag="SCM56_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG1110420.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(3741..4496)
                     /locus_tag="SCM56_001640"
     CDS             complement(3741..4496)
                     /locus_tag="SCM56_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1110421.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(4493..5452)
                     /locus_tag="SCM56_001641"
     CDS             complement(4493..5452)
                     /locus_tag="SCM56_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEG1110422.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(5445..6395)
                     /locus_tag="SCM56_001642"
     CDS             complement(5445..6395)
                     /locus_tag="SCM56_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEG1110423.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            6545..6997
                     /locus_tag="SCM56_001643"
     CDS             6545..6997
                     /locus_tag="SCM56_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEG1110424.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(7086..9353)
                     /gene="gshAB"
                     /locus_tag="SCM56_001644"
     CDS             complement(7086..9353)
                     /gene="gshAB"
                     /locus_tag="SCM56_001644"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HEG1110425.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(9540..10538)
                     /gene="ftsY"
                     /locus_tag="SCM56_001645"
     CDS             complement(9540..10538)
                     /gene="ftsY"
                     /locus_tag="SCM56_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEG1110426.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(10549..11358)
                     /locus_tag="SCM56_001646"
     CDS             complement(10549..11358)
                     /locus_tag="SCM56_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG1110427.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(11367..14948)
                     /gene="smc"
                     /locus_tag="SCM56_001647"
     CDS             complement(11367..14948)
                     /gene="smc"
                     /locus_tag="SCM56_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEG1110428.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(14968..15654)
                     /locus_tag="SCM56_001648"
     CDS             complement(14968..15654)
                     /locus_tag="SCM56_001648"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEG1110429.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(16002..17783)
                     /locus_tag="SCM56_001649"
     CDS             complement(16002..17783)
                     /locus_tag="SCM56_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG1110430.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(17809..18723)
                     /locus_tag="SCM56_001650"
     CDS             complement(17809..18723)
                     /locus_tag="SCM56_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1110431.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(18750..19712)
                     /locus_tag="SCM56_001651"
     CDS             complement(18750..19712)
                     /locus_tag="SCM56_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1110432.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(19712..20656)
                     /locus_tag="SCM56_001652"
     CDS             complement(19712..20656)
                     /locus_tag="SCM56_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1110433.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(20657..21661)
                     /locus_tag="SCM56_001653"
     CDS             complement(20657..21661)
                     /locus_tag="SCM56_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1110434.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
BASE COUNT         6121 a         4656 c         3863 g         7227 t
ORIGIN      
        1 tatatttttt tttgtgatta tcgttcaaaa aaagaataca cgcttgcatc tagtactctg
       61 cgagttacaa atcatccgat caaataaaca ttcgctgatt attttccgca aaaaaagcag
      121 catatccacg ataaatggat ctactgctct tacttatcct tatgttttac aaggtatttt
      181 cttttacaca ttactaatgc tgtctcaata cggaacgatt gtcatcgctt gttcaaattc
      241 ctcttgcaaa tgttgtgttt gataggcctg aaaagctggg tgatgggcac gcaagaggac
      301 acgtaatttc ataggatcat agctgtctag caactgttct ttcaagacaa aatctcgggt
      361 cagctgccat tcataataat aaccaaaagg tctgcagctg taaatcgtgt gaccgatgga
      421 tttatcttca aagcgacgat gcgtatggcc aaaaactact tgtttgatat ttgaataacg
      481 gtcaagtaat tctccgaatg attctgatcc taaaaatgca tttaatttgt tccaacgctc
      541 ataaggagca gattgataca tgataaatgc ccgtttagga acgaaatgag tggataaaat
      601 cacctcaagt ttttttgctt ccagctggtc tagcaaattt tttgcagctg caagcatttc
      661 tgcattgacc tcgagatctg ttttcccacg ctggatgaac cgatcatacc aatacaaacg
      721 cttcattcgt acgattgcct cgtaatcttt caattcggaa aactgatagt cgtaccagcc
      781 attcatccct aaaagaacgg tctgctcatt taaagcaata taccgttcat ttaaaaaatg
      841 tgggtctgga aaatggtcca tcatctgttc attgggcaca tctgccaact cgtgattgcc
      901 gaaatgaaat gtcgtagata atcctttatt gacgaaaaat tcgacaacag ctaggcaacg
      961 gtctacttta ttagccgtat ctccggctag atgaagccga gtgatcttgt gcttttgcaa
     1021 aacttgccaa agctgcatca gctctgattc gccgaattta ttgatgtcca catgtaggtc
     1081 gctgatgatc gccaattttc ccattttatc cctctagtct tttcgaacaa cttgcaagtt
     1141 gccttttctc aagccgcttg gtccttttaa ttgtaaacta ttataagttg gttctaaagc
     1201 aagtgtggat ggatcatcca tgtaaagtat tgcactgtct ttgattttga ttggtccaac
     1261 tggtgtatca gccactacag aataaagact tagatctgtt tgtgtatctt ttttgacaat
     1321 gatcaatcga aatgccgtat ccaattggca actgacttgg ctttctgcaa atggaccgtc
     1381 accgttttcg atatctaata aaagttgatc gtctgcttct aatttattct tcaatacttc
     1441 ttgtgcttct tttgtcactt ctaatttcat ctgtatcacc tcatgtcata gaataatcac
     1501 tttattcatc agatgcaact aatttgtctg gttctgactg ttctcaattt attggaaagt
     1561 aagtcattcg tttattatga tttctcttat cacaaaagag acaactttcc cttttcttcg
     1621 ttcagtttct tgaacggaaa aagacccaaa aagcaacctg ttatttgttt aaccttattc
     1681 ctttcttaaa taacaaaaaa tactataaaa aataaaaaac ctttaacaac tattggaaaa
     1741 agaattactt tttgttcacg gattattaac aactcttcgg tagagtaaac ttatcaaata
     1801 aaagacttct ttggaggaat cactatgaag gaatatataa aaaacatcta ttttatcgaa
     1861 gaaacacaaa atatcgaggg ttcttatatt gaagtaaaaa cactctttgt caatgaagat
     1921 aaaaccaaag cattggatat ttataaaaaa ttagcttcaa aaaaaacgaa tagttttgga
     1981 ttgatattaa gcgagtataa aatcaaagca gaagagagtt atttctatca attattaaaa
     2041 cggtggagta agcttcccgc cgatttttat cgaaaaatgc agatcatcaa ctatcaaccg
     2101 cttgctgaga cacatgccta aatagaggtt gtgaaatcag tgctttgagc taataccgaa
     2161 ggtcagctga ttgaacattg tttagaagag attgtgacaa ctgcgatttg ctttgagtaa
     2221 taagaacgct ggacagaaaa tagcttctca tattttctag tctagtgggc ttattgccga
     2281 gaagcagcag atggaacatc gtttagaaga gattgtgaca actgcgattt gctttgagta
     2341 ataagaacgc tggacagaaa atagcttctc atattttcta gtctagtgga cttattgccg
     2401 agaagcagca gatggaacat cgtttaaaga agagatcggt acaaaataaa aatatctaaa
     2461 tttaagtttt atctaagttt cgctttgtac ctttgattca aatcaaagga ggcgatcttg
     2521 atgctgaaaa aatggatcac actatttttt gccactcccc attctagaat caacccgtat
     2581 gatgtagata atgaattcac gttgaaaaaa gatttctcaa ataaataaaa accatccgta
     2641 aaatcatcaa tacgatttta cggatggttt ttgataactt ctttgaggat aggctcttgc
     2701 tatttaagaa tgtaagaaac attattgtta catagtatga gagtttaatc ctctgttatt
     2761 tatgctattt aaatgcttga ttcacatctt cgatcatcag tttcattgat tctaaaccgc
     2821 ccccgcttaa ataccagaca tctggttcaa gggagatgat ctgctgattt ttaccagcat
     2881 tcgtttgtgc taccagttca ttggcagaaa tatcatcttt gctgtcgtct ccaccaatag
     2941 cttttgttct gtctactaca aataagatat ccggattttt ttccagcaca tattcataag
     3001 aaacactttg accgtgagtg gatgcttcga tttgatcatc tgcttgttca aagccaaata
     3061 aatcatggat gaaaccgaat ctagaaccag atccgtaggc tgataattgc ccttcattca
     3121 caagtgtgac tagtgctttt ttgtcagtag cttctgcttt ctttttcgtc tgatcgattg
     3181 cactagataa ttcctctaat tgagttttag cttcttcttg cttaccaaag atctccccta
     3241 aagcagtgac attttgctgg aaagattccc atggattttt cgcatctaaa gaaaggaaaa
     3301 tggtcggagc aatttgactt agatcttttt gaaaatctct ttgtcggcca gaaatgatga
     3361 ttagatctgg ctgaagctga ttaatttttt ctaagtctgg ttcttttatt ccacctgctg
     3421 attctacttt tttataggat gaaagatact caggaaggct gcttgtagct gctccaacta
     3481 ccttatctcc aacaccaaga gcatccatcg tatctaaaga accattgtcg aatacgacga
     3541 ctttttctgg attatttggc acctcgattt ggtctccatt actatctgtt atcgtcaatg
     3601 tagtttcagt ttgtgtgctg cttttttttg tgtctgcctg actggtgttg ttgctatttc
     3661 cacatgctgc taaaccaaat gatgctagaa tcaagatacc tactactaat tttttcatct
     3721 cttctctcct aatgttagtt ttagaaataa agacagaatc tgcggccttc aatctcacaa
     3781 atccgtatgt ccatttcgta taattcatcc aatacatctt tttttatcat ttcttgggtc
     3841 gttccttgcg taaataactg tccatctttc attgcaacaa tttcgtctgc ataacttgca
     3901 gcaaagttga tatcatgaag aacaataaca attgttttcc cgatttcttg tactaaattt
     3961 tttaaaatct gcatgatttg aaccgcaaaa ttcatatcta gattgttcaa aggttcatcc
     4021 agtaaaatat aatccgtgtc ttgcgctagc accattgcaa tatacgcacg ttggagttga
     4081 ccgcctgaaa gagtgtcaat cgattgatcc gctatttctt ctaacccaag gtgttccagt
     4141 gattcctgaa cgatttcctc gtcttttgtc gtcaattttc ctttcgtata aggaaatcgt
     4201 ccaaattcta ctaattcttt cacggtgatt tggacattta tttcattcga ttgtcgtagg
     4261 atcgataatt ttttggctaa ttcattggat ttccaagcct ttacttcgtt atggtctaaa
     4321 tagatttcac ctgtatcttt tggtatcaaa cgactgatca tcgacaacac cgttgacttt
     4381 ccagcaccat ttggaccgat aaaagcagtc aatttctttt caggaatcgt taactgtaca
     4441 tctttaacca ctgattttcc atggtacagt ttattgacct tttgtaaatc aatcatagga
     4501 tactcttcct ttcgcgaatc aattttgtga caaaataaat tcctccgaca aattcaacga
     4561 cgacactcac tgttgtattc cagccgaaaa tctgctctac tgcgagctgt cctcctgtca
     4621 gaaacagcat agctaccaga aagcttccga taaatagctg accatgacga taagtagaaa
     4681 agacacgata actgatattt gcgacaataa accctaaaaa agtaactgga cccacaagag
     4741 ccgttgatgt ccctgtcaaa agactgacac aacataaaat caaaaattca aaacgatctg
     4801 tgtctatccc gaggtttact gcttgttcat ttccgagatg gaaaacgtct agcacatgtc
     4861 gatatcgaaa taacaggtac cagcagccga taatgatcaa agaagcaggc aaaagatact
     4921 gactctggat attgccaaag cttgcaaata atctgccttg aagcaaatca tattcattcg
     4981 gatccatgat gacttgtaaa aaggtactga cactaccaaa aagcgtacct aaaatcatcc
     5041 ctgacatcag taataaaaaa agattcgctc tcattttttt caaaagaaag aaaaataaca
     5101 atagactcag cccgaccatc agcccaacgt tcaataagaa aaaaactgta cctgtctgct
     5161 gaagcatcgt cactcctccg ataaagaaga aaaggaccgt ttggacaagg acatataacg
     5221 actcaagtcc caatactgaa ggagtcaaaa actggttatg cgtaattgtc tgaaagctga
     5281 ttgtggcact gctgacagct agcgagacaa ggataaatgc aaaaattttc ttactcctca
     5341 atgcacatgc aaaatgccag tttccatatg tatggatcgt caagtacatt cctgtaacaa
     5401 ataaaacaag aacaagcata ccaataaaaa tagatggctt ttttttaagc atatcgtcgt
     5461 ccccttatca gaagataaag gaagatagcc ccaccgatga ttccaacgat cacactgact
     5521 ggtatttcat aaggaaaaat caacacgcga gcgacaagat cgcaaaatac taagaacaaa
     5581 ctacctgcta aagcagttac gccaattgtt ttgtgcactt gatctccata tattctagag
     5641 acaagattcg gaatgatcac tccgataaaa ggaatgcttc ctaccatgat caacatgact
     5701 gcactagcca atgatactaa gccaatgcct aaaaactgca tcttctggta atctattccc
     5761 agatttactg caaacgattc gcccagacca atgatcgtaa atcggtaagc aaatagccaa
     5821 accaagagca acaatggaac aacagcatac aataactcat aatttcctcg tatcacagtc
     5881 gcaaaattcc cttgaagcca tgaagtcata ttttgtacca actggaattg ataagcaaaa
     5941 aaagtagcaa ctgaaccaat gatattccct aacatgactc caatcaacgg aagcatcaat
     6001 tgatttttct ggaggaacaa tcgcgtaagg gataaaaaaa gcaatgttcc tgcaaaagca
     6061 aagcaaaatg ctgtcaagga tcggacaatc aaggatgcac caggtaggaa aatcatagcc
     6121 actaaaatac ctaaacgcgc actatccatc gtgcccgcag tcgttggtga aacaaattta
     6181 ttttgggtca aatgttgcat gatcaaccca caaatactac tggtcgctcc cacaaggatc
     6241 aaacaaatcg ttcgtggcaa acgagtcact agcaagattt gtaagctttg cccatctaac
     6301 tggagcagtt ctgacaattg tacattcgat actccgatga acaatgaaat actacttaaa
     6361 ataactaata atgctactag tcgtccgtag cgcatccaat ccacctttct aattgagaat
     6421 gattttcatt cttattttaa gtgaatcgtt gaacaaaagc aaccgctttt tcatatgttt
     6481 taggtattat tgggaatatg ctataatcgt aagaaagaca aagagaagaa ggagggtatt
     6541 ttttatggca caagatgatg taaaaagcca tttggataag ggactttatg gtacaccttt
     6601 agtgaaccct gaagaacagc ataaatattt agggacattc agggaacgct gctatcttag
     6661 tatgactgtt gctgaaatga tccaaccaaa aaacaaagaa aactttttaa aagaactcaa
     6721 aaaacatcca gatgcttcca tactcttgaa tggggctcta cccctttctg tccaaaacca
     6781 atatatccag ctagcaacga aaaacaatag ccgtttcact gtagtcaacg attacgtcac
     6841 tgcaaatcct gaggaattcg gattgctctt aacagcaaaa gaagcagtaa atgaacctgt
     6901 gattgatatc gaaaaaaaat acccagaaga aaactcttcg ccagctgaaa aagaaaaacc
     6961 aaaaaagaaa aaaagcttct gggatcgttt attttaaaac gaaaaaacac ataaattttt
     7021 acatgcaaaa aaagcttttg atcagattga catcaaatag gacaatctgt caaaagcttt
     7081 ttcttttaaa atacttcagg atataacaat ttcaatacat tcatcgtcaa tcttctgcct
     7141 tttcctgcaa aaggataaac atgcatatgc atggctggat tgaaatttgc ttcgatgatc
     7201 ccatatgctt ttttatcagt tgtaggatcg atttctttat ctggtataat caaatcgata
     7261 ccactaatct tagctcctag tgcttctacc gcagaaacgg cgattttttt gtaagcttca
     7321 gaaaattcat cagtcatatc gatcgaatca ccacctgtac tgatatttga gttctccctt
     7381 agataaacaa tttgattggc ttggggaact gattcgatcg tcaacccttg ttctttcaac
     7441 atcaattgtt ctaattcacc taattggatc aattctaaag gtgcacgatg attcgttcca
     7501 cgcaaagggt cactattttt ttcttcaaca agttctttca ctgttcggat actgtcacca
     7561 ataacattag cagggactcg caacatgatg gcttggactt ctccgtcgat cacaaagaaa
     7621 cgatattccg ttcccggcat aaattcttct actaaaaccg aactgtcttc acgaaaagca
     7681 atggctaaac ctgcttgata atcttccaga ctagctcctt ccttgaaaat cgtgatacct
     7741 aacccgtaat tggttgattt aggtttgatc acaaatgcct gctcggcaaa tctcggataa
     7801 gcttttaccg cttcttccat ggatgaaaat tctgcgcctc ctggtacccg gaacccagct
     7861 tcttttaaga cttttttcgt tactgttttg ttttccatga taagtgggac gatatagctg
     7921 tccttacttg tcatgtttgc atttttcaca tattctacat ggtcttggaa ttgcagacgc
     7981 aaaaattgat ccgactcatc taatactttg acttggacac ctttttggat ggcatcgaac
     8041 ataaatatct gagtagaaag ttccatttcg cgaaaaccgg ccaactgata gggacgttca
     8101 tggctttctt tataatattg atgtcccaat gccacagcaa cttcttgctg actcgatagc
     8161 tgtgcttctt gatagagttt cgctgctaac gtttgttcag gatgatccat ttgagtcaat
     8221 gcttcctcta ctaaataaag agaatccaac caatccaatt caaccagcat ttgcttcatt
     8281 ccttcaagaa tttctctacc ttcttgatga aattctgttt gatctgatgg ttgttctagt
     8341 gctactttgt tatttcgagt cgtaccttct gctacccatt ggtcacagtc ttctttttct
     8401 tctgtccata acatgtacat caggaataat tgtaagaacc gaacttgctc tggattgata
     8461 cccaatcttg cataaggatt caaatcgata ttccgcaatt caatataacg aacacctgtt
     8521 gttgctaaat ctgcgacttt ctttccgcct cggaaacgaa gcggtgtata aaattctttc
     8581 tcctccgaaa gtttcccttc ttcgatcatt cgctcgatat ctgctaaata ctgtttcata
     8641 gaagcatagg aaactttcac atttggatga ttcgtatagc ccagcgcact gttgcgaata
     8701 ctgcgaacag gttcttgcgg atgtgattca tctaaaaagt agttcttctc actcattggt
     8761 gaagcaccga ataaataagt gatcatccag cgatagcgca taaagtttct cgctgttttc
     8821 aaataaagtt ctgttttgaa ttcggaaaaa tcttgaaatt cttcctgatg gctgaacaat
     8881 gttcttagca actcatcgcc aaattcaaaa ttgaaatgaa taccgcttac catctgtttt
     8941 cgcttcccgt attcttttgc taaataacgt cgatacaaga catcttcgaa attttttaat
     9001 tttgcaataa tgatctcttc gtctttttcc ggtaaggcag gtggcatact taatggccag
     9061 atcatctctt ctttcggtat agaacgagca gtcacatcat aaacagcagc gagatactga
     9121 aataattcgg gaatagaatc ggtaacaggg gtgatcatct ctgtttgtgt ttcgctaaaa
     9181 tctgtttgga tataggggtg ataggatcga tcgccaaaga ttgctggatg atcggttttt
     9241 gctagatttc ctgcaagatc aacccgctgt ccttcccgtt ctataccaaa tcgagcttgg
     9301 tcgataaaag ggcgcgcatt gacatgcaat aataattgct taaaattcat cattaagtga
     9361 cctccaattc atatgaatct aattataatc aaaaagtcta agaaaaagaa cgaaattgtt
     9421 tgagaaccgc ttacaaataa gtataaaaat gaaggcatca aaaacacagc tatgttaaaa
     9481 acaatcattc aaacaaggag ctgcaacttt tgtcacagct cctgcttttt agacatgggt
     9541 tattcttctt ttagtaatcc tttgaacaat ccaacgacaa aatcatttgg atcaaacggc
     9601 tccaagtcat cgattccttc accaagaccg accaatttca ctggtaaatg caattcatta
     9661 cggatagcaa gtacgatacc gccctttgct gtaccatcta attttgtcaa aacaagaccg
     9721 gtcacatctg ttgtttcttt gaattgtttt gcttgaacca tcgcgttttg accagtggtt
     9781 gcatctaata caagaagtgt ttcttggggt gcttctggta attcccgctg gattactcgt
     9841 tttatttttt ctaattcgtt catcaagttc actttatttt gcagtcggcc tgcagtatca
     9901 actagtaaaa catctgcctg ctctgtcttt gctctttcca aagcatcgaa tacgacagct
     9961 gctggatcgc ctcctgcatt gccacgaaca acttcaacac cagcgcgttc gccccagaca
    10021 actaactggt cgattgctcc agcgcggaag gtatctgctg ctgccatcaa tactttttta
    10081 ccttcgtttt tgaactgatg ggcaagttta ccaatacttg ttgttttccc tacaccatta
    10141 acaccgacaa acaaaataac tgttagtccg tcttcttgga tatttagttc atttacttca
    10201 ttgattcctt ccgcttcata gagatccaca agtttctcga tgatcgcatt ttggacttct
    10261 gccgattttt tcgcatttct gagtttcact tcttgacgta atgcttcggt aattcgtatc
    10321 gctgtttcaa aacctacatc tgcaccgata agtgtttctt ctaattcttc aaaaaagtct
    10381 tcatcgactg ttcgaaaatt ggcaaacaac tcattcatcc gttcaccaaa tgttttacgc
    10441 gtcttttcta aacccttgtc gtacttctcc tgtacttcaa cttcttctgt tttctcttcg
    10501 ccagtaaaag cacgcttgat tttatcaaaa aatcccattt gactccccct actctaatac
    10561 aaatttttgg atcactttgg ctacgccatt ttcttcattg ctggctgtca cgaaatctgc
    10621 tgcttctttg acgaaaggca cagcattctc catcgctacg cctagtccag catattggat
    10681 catcggtaaa tcattttcct catcaccgat cgccatgatt tcttcttgtt tcaaaccaag
    10741 atcttttgcc aataaagaaa taccataagc ttttgtaaca ccttttggca taaattccaa
    10801 aagcatactt cttgttttga tgatctcata catttcatgg aattctgctg gtatttcttt
    10861 gattttagga tctaattctt cttggtcata agcgattact gccttattaa gcacaaattc
    10921 atctgtaata tcgcttatat ccgctggttg gaattgcagc agcttattca acacattgta
    10981 caaggattgc ttccttgggg cactaggcag ctgtaaaacc acattgtcag acaaaacatc
    11041 tagtggcaga tccaattgct gagccagttt gtataagcgt tggatatctg atacttccat
    11101 cactttcttt tccatgattt caccagtatc atttttttgt accaaaccgc cgttgaaagt
    11161 gatgctgtag tcaccttcat ctaccagtcc taattcttgc agataatgcg ccatcgcagc
    11221 taaggggcgt cctgtacata gaacgatctt gaccccttgc tgttttgctt gtgctaatgc
    11281 ttgtttattc tcatctgaaa tcagcttttg ttcatttaat aatgtaccgt ccaaatcgat
    11341 tgctactaat ttgatcattt tcgttcctac actttcgttt cgttgatttt cgcctcttca
    11401 ttgatatctt ccatccgtac agaaacgatt tttgatacac cggattcctg catcgtcact
    11461 ccgtacagta catctgctgc gaccatcgtt cctttgcggt gtgtgactac gataaattgt
    11521 gtgtcatctt ggaaatcgct caagtaacga ccaaaacgtt tcacgttggc ttcatctaac
    11581 gctgcttcta cctcatctaa gatacaaaac ggaacaggac agacacggat aatggaaaag
    11641 agcaacgcga tggctgtaag tgctcgttct cctccagaaa gcaaacttag actttgcagt
    11701 tttttgccag gaggttgtac ttcaatctca atacctgttt tcagcaaatc agttggttcc
    11761 gtcagtacta attcagcttt accgccgcca aacatatttg gaaagacaat tttgaattct
    11821 tgacggatag cttcaaatac ttctttgaat cttgtccgca cttcatcatc catctcatcc
    11881 atcgtttcaa acaattggtt tttcgctgct aacagatcat ctcgctgtgt tgctaagaat
    11941 gtatgtcttt cactgacttg ttcgtattgt tcgattgaat ttaagtttac tggtcccagt
    12001 ttttcaatct gttcttttaa ggatgaaacc tttgttctac tgtcttcgat atcggttgtt
    12061 tcttgatagt cttgggaagc tttctcaaat gtcagctgat attctgactg cagataggtc
    12121 aaatgattat ctaaaatcat ttcagctcgg tctttttgga cttctaattt ggattgttcc
    12181 gacagtcgtg ctttttgctc acgattacgc tctgccaaga ctgcttcaag cttatcgatc
    12241 tccttttgcc ttctatctcg ctgctctttg gcttttgcca actcggcttt taatgtttct
    12301 cgctgagcag ataattcgtt aatttgtttt tccaaacttt cttctgtgac ttcatgatca
    12361 gaaaagtctg ctgtcaatgt cgccaactgc ttttctatcg cttcttgacg ttcggttgcc
    12421 tctgcttttt ggactcgggc accgcggagc tggatctgca gatgattaaa ctgctctttc
    12481 aagacagcaa gatctgcttg ttcttgtgct ttttgacttt gtacttgtgc tcttctggct
    12541 tccatctggt cgctttcttg acttaacgat ttgatttctt cgtcgatttt ttgtcgttgc
    12601 gattcaagat ctgtttgttt gtctttcaat tcgttcgttt gcttttcgta atcttcgata
    12661 aactgctgca gttcacgtgt ctcaaaagtt gagatttgct tttctttttc aaaacgttca
    12721 agttcattta tgatgttttg tagctgattt gtcgcttctt gttcttcaaa acgtaattgt
    12781 tcgcctcgtg tacgcaaaac ttcttgttct tcggctaaac gagctgtttc ttgttgcagc
    12841 tcctgaactt ttttttcttg tgcttggagt tgtttatctg cttcttcaaa ttcactagtc
    12901 aactgtttca attcttgatt ttgtacaaac aaactgcctt ggtttcctcg tttattcgct
    12961 ccacctgtca tggatcctcc ggcattcatc acatcacctt ctaaggaaac cactcgatat
    13021 tgatagcgga tcgtttgcgc gattgcattg gcactagtta agtctttggc taaaaggatc
    13081 gttcctagca ggttttgaac cacagtttgg atttgatcgg gataactgac ttgttcactg
    13141 gcaatcccta taaacccttc tacagctgct gcttgtgtca atatatgagc cggtaattgt
    13201 cttggtttga ttgtcgtcag tggaaggaat gttgctcttc ctcctctttg ctgtttcaaa
    13261 tacgtgatgg cttgccgagc atctttttca ttttcaacga tgacatgctg ggctgcccca
    13321 cctaaagctg tttcgatagc taacgtgaaa tctgcaggaa catcgatcag ttcagccact
    13381 gctccgacaa tacccgaaag ctgctgttta tgctgcaaga ccaatcgaac cccttggtaa
    13441 aaaccaaaat agttttcttg gatttcttga aggcttttct tacgagcacg tagctgctgg
    13501 acttgattca ttaattgata cattttaggc tgttcttttg ctaatgcttc ttggaacttt
    13561 ttcccgtttt gttggatcat ttctaacttc tcttttgttt cagttaaaga tgattttagc
    13621 tgcgcctgct tttgtgtcaa ttcttctttt tgtaagatca atcggtctac tgatgcttct
    13681 acttccgatt gcttggctaa cgtctgcttg ctttttgctg tttcttgtat atattgacgt
    13741 tctagatact tcagttcgtt tcctacagct gcttgttctt gcatcaaatc aacatattga
    13801 tctcttaatt ctgccaataa ttctttcgtt gatttttggt atttttctaa ttcttgctgt
    13861 gtttttatga gatttgcttc agctttttgg atctctgttt ctttttctgc tgctgctttc
    13921 atcaagcttt cttgaagctt ttcaaaatgt ttaacctttt tttgtgcttc tgctaatgaa
    13981 gtttgatatt cttgcgagct tttttgtgta tgctttgtcc gctcctgcaa aacatctttt
    14041 tgtccttctg tctgcttcag tttttccgaa agatctaata atacttgctg atttttttcg
    14101 atcagacgat cggcttgcgc attttcttta cgctgcttcg ccaaaatgga ttcttgttct
    14161 tggatcgact cagatagttt gcctaattct agattgaatt tcgcaagctg tgcttgtttg
    14221 ttgtcccaat ctttttttgc tgtcttgatt tcagcaacca tcaaagagac atcggtttga
    14281 gtcagtgttt cttttagccg taaaaactcc tttgctgcct cactctgggc ggccaaagga
    14341 gttaactgtt cttccaattc gtggatgatg tcttggacgc ggctgaggtt gtcttctgtt
    14401 tcaaaaagct tctgttctgc tttctttttc cgctgcttgt atttcaagac accagctgct
    14461 tcttcaaaga tcccacgacg gtcttccggt ttactactga aaatcgcttc tacttttcct
    14521 tgagaaatga ttgagaacga ttcttttcct aatccagaat ctaagaacaa ttcttggata
    14581 tcttttaatc tgcagctttg tttattgatg aaaaactcgc tttccccagt ccgtcgataa
    14641 cggcgggtta cactgatctc attgtactcc aatggcagat aatgatcact attgtctaaa
    14701 atcactgtga cttctgcaat attcagctgc ttacgggtat cggaaccggc aaagatgata
    14761 tccggcattt ttccgcctcg cagactttta gcagattgtt ctcctaatac ccagcggata
    14821 gcctctgtaa tattgctttt tccgctgcca tttggtccaa ccactgctgt gacgctattt
    14881 tcaaaatcaa tgactgtctt gtctgcaaat gatttaaaac cggctatttc aattcgtttc
    14941 aaatacaaag ctttccctca cttactttta cagtgctgcc aatgcatttt ccgccgcagc
    15001 ttgttctgct aattttttcg atttcccttg tccagtgcca atcaattgat catctacata
    15061 aacttcgatc caaaatactc gttcatgagc tggaccttct tcattgatca atcggtattc
    15121 gattgataca tcacctgatt tttgcaagac ttcttgtagt tttgttttat gatccatctc
    15181 atgtgaaaaa gcacctgctt tgactttagg aaaaatcact ttttcgatga atgcatgggt
    15241 cgtgtcgaat ccctggtcaa gatataacgc gcctaaaaaa gcttcgaata ggtcgcaaag
    15301 caatgctggt cgcgtacgtc ctcctgaatt ctcttctccc ttgcccaaca aaacatattg
    15361 atcaaagtga cattctttgg caaatttcga caagctgtct tcgcgaacga tcgcagctcg
    15421 tgtttttgtc aattttcctt ctggcaaatg aggatataaa cggaataaat actgagaaac
    15481 catcagttct aaaacggcat ctcctaaaaa ttcaagtcgc tcattatccg ataattgcag
    15541 attgcgatgc tcattcacat aggatgaatg ggtaaaagct tgttcaagca aatttagatc
    15601 gtgaaaaacg atgttatagt tttcttttag ttcttttgtc agctggttgt ccattttcca
    15661 cattcctttt atggatattt tttcgatact tccttattat actggttttt tgtcaaattt
    15721 gaaatggttc ggtcaaaagg taagtaaagc tttaataatc ctagtgattg acgagaagtt
    15781 gtgaaaaatt cgtttagttt cgagtatgaa gaaaaaattt tgtgaaaatg gctttccata
    15841 tttttgcata aatttgactt aataccgaga aactggactt tgaacaccgt ttattggaga
    15901 agtcatgaaa tcagcgcctt gacctgaata ttagagaaat aaacagaaaa atatcaaaaa
    15961 accgaaaaag gacatcgccc tttttcggtc atttttagtg attatgaacg tgaatctgct
    16021 gtaacagaaa catttgccca tgggtttggt acatcataag accaatccca gtctttgaca
    16081 cggtcgttga ctggcaatac ttcattacgg aacaacgttg gaatcgcaaa tgcttcttca
    16141 aatgcatatt gttgccattt ttgatatgct tctttttggt actcttcatc aaatgctttg
    16201 cttgaactga tatcgttgat caattttgta ttttcttctg attcataacg agtgtagttg
    16261 aacgcagcat ttggtcccca tagtccactt ggatttggat cagttcctgt attccaagca
    16321 gcttggtaaa catcaatttc aggatcatcg ttttctaatt tatcatagaa agaattgaat
    16381 tcgatcagac gacctgttgt gtattggaca tccaaaccaa tttctttcca ttgttggata
    16441 tagtaatctg ccaatggttg cgctgtttcc ccaccggaca tcgaagcaaa gttgattgtt
    16501 agtttatttc catctttgtc ttctcttaaa ccatcgtcat ccacatcttt gtagccagca
    16561 tcatctaata gttttttcgc tttgtctaga tcttgtgtga atcctttcat ctcatcatca
    16621 tggtatgact ggaatactgg aggaatcaat gaagtcgcat tgcttctcaa acctgcatag
    16681 aaacgttcac ctactgcatt attatctaat gcatagccca ttgcttgacg taatgattta
    16741 tcagccattt ttgaatctgg attgtattcc acactctttg tatctgcatt ccatttacct
    16801 aacttgaatc ctagataagt ataagaagtt tgttcacggc ctaagtttgt atagccagca
    16861 atttctttat aggtatcgta agtatctgtt ggcatttgga aaatcatatc atataattta
    16921 gagttcaatg cttcagttgc acttgccatc ggtacagatt tcatcgtgat ctttttcaat
    16981 ttaggcgtac cattccagta gtgctcgttt ggcaagtatt ctactgattc tcctgcaacg
    17041 atattactca tgtaataagg gccaaaagtc actggatttt tacgtacttg atcgcttgat
    17101 tccatatctt tgattgctac accttctaac gtatgttttg gtgctgcata tgaccagata
    17161 ccaccacccg attgaagcat gcttgggttt acttctttat attcgatttc aactgttttg
    17221 tcgtctattt ttttgattcc ggagattgtg tcggcacttc catcatgata ttcttccata
    17281 cctacgatgt tacggaaagt gtcgtcataa cggataccag tgtaatcttt attcccaatg
    17341 atttcgtaag ggaaaatcac atcgtccgcc gttacatctt caccatctga ccatttgacg
    17401 ttgtctttta ctttgattgt cgcttttttc gtatcttcat ctagatcaag cgttgcagct
    17461 ccgtcgtctg tgattctgaa atcgtcatcg tttgcaaata gcggttcatg agaaggagcc
    17521 ataaaagctg catcatatga atcttggtaa aactcccact ggaacaatcc tttaaactga
    17581 ctgtccgttg cgaccgcagc ttctaattcg ccgtcttcga ttgcatcatc attatttttt
    17641 gttgcaattg gaaaagttaa ttcctctttg ttattgccgt tgcctccaga gttgtttcca
    17701 gaggtatctg tacctccgcc cccgcatgct gcaagaacga cagcagacat cagcgctgca
    17761 aatccaaaaa tcgttttctt cattcctatt tcccccttcg cttctatact agcctaaccg
    17821 ttggcgcgca tctgcagaac gcttgaatgc ttgtccaaca taattgatac tcaacatcaa
    17881 aactaaaata aggattgatg cgggtaacca gatccattgc cgattcacta agacttctcc
    17941 gttacttgca taaccgatca atgtacctaa actaggtaca tttgccggca agccgaaacc
    18001 taagaaagtc aaagttgtct cgataccgat gtttgccgca aagttcatcg tcaaattagt
    18061 aatgattaat gaacttagat tcggcatgat ttcgcggaac atgattttaa aatcattggt
    18121 tcccattgtt tttgaggcac tgacataatc tcttcttgct tcagataatg tcttgcttcg
    18181 gaataatcgg gctttagcta cccaataaaa tgcactcatg atgaatataa aggaccagac
    18241 attgtatctg gaaataatcg atacgaagac gataatgatc agcatgatcg gtagaatcat
    18301 gataaaatca acgatccgca tcaagatgtt gtcaatgatc cctccgtagt agccagagat
    18361 gatccctaat ccaaccccga tgatcgaagt aaggatcgtg atcgcaaatc cgatcgtaac
    18421 agagtttcta gcacctatga tcaattgtcc taatacatca cgtccgcctt catctgctcc
    18481 cagaatgaac ttttgtcctt gtgtcgaaac tgttcctggt gcagcgtatt tgtcgaagat
    18541 actaacatac atcactttgt cttgatcggt cagcatcgca ccgataaaga tcactaataa
    18601 taaaaccact agcaaaccta atgaaaaaat agcaatttta tcttttttaa attctcgagc
    18661 aatcattcgt atccccatag gaggaatact ttcttgaact gctgtattgt ttttttcact
    18721 catcgatcca ttctcctttc tgattggttt tattgaatcc gaatacgcgg gtcaacgata
    18781 ctcatgatga tatctgaaag taatgtcccc actagcgtcg cgattcctaa aatcaatact
    18841 aaggatgtga tcacggtata gtcccgttgg ccgatggaat caataaatag ttttccgact
    18901 cccggataac caaaaatctt ttcgataact acagaaccag caatcaacga agtgatttca
    18961 taccccattt gagaagccat cggcaacgct gcatttcgga aaatatgtcg actgaatact
    19021 ttattcgtcg gtaccccttt ggaacgggct gttctgacaa aatcgagcga tttcgaatcg
    19081 attacttcac ttcgcagata ttggattgtg acggcagtcg ccaaaaatgc ttgggtaatt
    19141 gatggtaaca gaatatattt caaacggtct atccattgag tgataaatcc accttgcaaa
    19201 cctagtgttt gtgatccact tgttgggaac cagcctaaac gataaccaaa aacaaatagc
    19261 atgatcaagc cgaagataaa taaaggaaca gcaaaactaa agaagttgta gattacgaca
    19321 atcttatcca agattgaatt ttgataccgg ccagcaagca tccccagagg cacagcaatc
    19381 aaataagtaa ggacaactgt caagatcgat agatataaag tgttgatgat ccgttcgccg
    19441 atcaagtcag caacagagcg tttgtataag aaactttgac caaaatcgcc ttgcaaagca
    19501 tttccgatcc aacggaagta ctggacatac catgggtcat tcaagccagc tgcttgtcgc
    19561 atctgttcga tcactgccgg atcctgattt ggattgatca aaccggtaaa tggatccccc
    19621 ggcatcgctt gggctaataa gaagatcaga acacttaaaa taataacttg cgggatcatc
    19681 aataaaacac gtcgcagaat cgttttccac attagcttgc acctccatct ttcaacgcca
    19741 ctttgtgtgt agttgtgatc gtacgcaaat cgtagactcg accagtcgca tcatagtatt
    19801 ctttttggtt ttggatatac tcttgttcta ctcttcgtcg attttctttg tggctttccc
    19861 tattttctac atcgatttgc ggaatagctg aaagcagacg tttcgtatag atatgtcgcg
    19921 ggtcattata aatgtcttct cttgtaccga tttctacaaa tcgacctttg tacataattg
    19981 cgatattgtc gcacatgtgt tttactacac ctaaatcatg tgaaatgaat aaatagctga
    20041 ggccgtattc ttgctggatt cgtttcatga aattcaatac ttgcgcttga acagatagat
    20101 ccagagcaga tacaggttca tcggctacga tcagttttgg attggtagca acagcacggg
    20161 ctactcctag acgttgacgt tgtccgcctg aaaattcatg aggatattta tatagcgcat
    20221 cttccggcat cccaacgata tctagcagtc cttttacctt tttcttttct tcttgatcgc
    20281 ttaaccgttc aaaatttcgg atgggttcag caatgatatc caacactcgc tttttaggat
    20341 tcaaactgga cattgaatct tggaagatca tctgcacatc cttgttataa cccatttttt
    20401 tgcggttgct tcgcttcgtt acatcttttc cttcatacat gatttgtccg gctgttacct
    20461 tttctaatcc cacaacggct tttcccgtag tagattttcc agagccagac tctcccacta
    20521 aaccatatgt ctttcctttt tcgataataa aatccacacc atctacagca tatacatgat
    20581 ccgttactcg attgaagaat ccactgcgaa tagggtaatg aactttcagg tctttgattg
    20641 tgattaattc tgtcatctac acctctcctt tttcgtctct aaagtggaaa tgcttatagc
    20701 aggagcaacg taccaagtgg ttcggtgcga cttcatgaag gataggctct tcttcatgat
    20761 cgcttgctgg tatccatgga atacgtggtg caaaccgaca acctttacga ggcatgtttt
    20821 tcagagaagg aacgacacct tcgatcacat ggagttctgc atcatgattt ccttcttgtg
    20881 gtatagagtt cagtaaagag cgtgtataag gatgcttagg atgatcaaac agttctttta
    20941 cacctgctac ttcaacgaac tgtccgccat acatcactgc cactcgatca gccatttctg
    21001 ctacgactcc taagtcatga gtgatcaaga tgatccctgt ttttgtttct tcctgaaggt
    21061 ctttcagtaa atcaagaatc tgtgcttgga tggttacatc tagggctgtc gtcggttcat
    21121 ctgcaatgac gattggaggt ttgcaggcga ttgcgattgc gatgatgaca cgttgtctca
    21181 taccgcctga tagttcatgg ggatattggc gtccaactct cgcaggatta ggaatcccta
    21241 cttgttctag caattctagt gcacgagctt gacgctgttc tgcattcatc tttgtgtgat
    21301 aggaaagtcc ttctttgatc tggtcttcga ttctcatgag aggatttaat gcagacaacg
    21361 gatcttggaa aatcattccg atatcatttc cgcggatttt attatagagc gtttcgttca
    21421 ggtcgattaa attcagatcg ttatataaaa tctcacctgt aatttttgtg ttattaggat
    21481 caagtaatcc cataatcgta gtagccaagg tacttttccc gcacccagat tccccaacga
    21541 ttgctaaaat ctcgtttttg tctaaagtaa atgaaacgtc atctactgca tcgtaatagt
    21601 catctttgag tcggaatcct gtatgtaggt tctgaacttc taataattgg ttatcggaca
    21661 atcgaatacc cctctctgct tcctaaatgg aagtttttct taataaaaaa ggacattaca
    21721 cagaataaat cgttttataa caaaggttat tcttatcttt gtgtaatgaa ttattaataa
    21781 ttatatagaa aaccgcttta ctttgcaaca taatttctgt ttatcgccag aattatcaga
    21841 aaaatgactt ttacgtgtca aaaagat
//
LOCUS       DARBDV010000048        21405 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000048 DARBDV010000000
VERSION     DARBDV010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.048"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            86..787
                     /locus_tag="SCM56_001654"
     CDS             86..787
                     /locus_tag="SCM56_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEG1110435.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1396..2412)
                     /gene="tsaD"
                     /locus_tag="SCM56_001655"
     CDS             complement(1396..2412)
                     /gene="tsaD"
                     /locus_tag="SCM56_001655"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEG1110436.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2409..2873)
                     /gene="rimI"
                     /locus_tag="SCM56_001656"
     CDS             complement(2409..2873)
                     /gene="rimI"
                     /locus_tag="SCM56_001656"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEG1110437.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2880..3422)
                     /gene="rimI"
                     /locus_tag="SCM56_001657"
     CDS             complement(2880..3422)
                     /gene="rimI"
                     /locus_tag="SCM56_001657"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEG1110438.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3406..4128)
                     /gene="tsaB"
                     /locus_tag="SCM56_001658"
     CDS             complement(3406..4128)
                     /gene="tsaB"
                     /locus_tag="SCM56_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEG1110439.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4319..5299)
                     /locus_tag="SCM56_001659"
     CDS             complement(4319..5299)
                     /locus_tag="SCM56_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1110440.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5323..6807)
                     /gene="galT"
                     /locus_tag="SCM56_001660"
     CDS             complement(5323..6807)
                     /gene="galT"
                     /locus_tag="SCM56_001660"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEG1110441.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6819..7808)
                     /gene="galE"
                     /locus_tag="SCM56_001661"
     CDS             complement(6819..7808)
                     /gene="galE"
                     /locus_tag="SCM56_001661"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEG1110442.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            8056..8223
                     /locus_tag="SCM56_001662"
     CDS             8056..8223
                     /locus_tag="SCM56_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEG1110443.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(8285..10096)
                     /locus_tag="SCM56_001663"
     CDS             complement(8285..10096)
                     /locus_tag="SCM56_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEG1110444.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(10093..10458)
                     /locus_tag="SCM56_001664"
     CDS             complement(10093..10458)
                     /locus_tag="SCM56_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEG1110445.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(10621..11016)
                     /locus_tag="SCM56_001665"
     CDS             complement(10621..11016)
                     /locus_tag="SCM56_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEG1110446.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(11034..11996)
                     /locus_tag="SCM56_001666"
     CDS             complement(11034..11996)
                     /locus_tag="SCM56_001666"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEG1110447.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(11996..12208)
                     /locus_tag="SCM56_001667"
     CDS             complement(11996..12208)
                     /locus_tag="SCM56_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEG1110448.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(12229..12927)
                     /locus_tag="SCM56_001668"
     CDS             complement(12229..12927)
                     /locus_tag="SCM56_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEG1110449.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(12947..13489)
                     /locus_tag="SCM56_001669"
     CDS             complement(12947..13489)
                     /locus_tag="SCM56_001669"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEG1110450.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(13621..14625)
                     /locus_tag="SCM56_001670"
     CDS             complement(13621..14625)
                     /locus_tag="SCM56_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEG1110451.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(14622..15611)
                     /locus_tag="SCM56_001671"
     CDS             complement(14622..15611)
                     /locus_tag="SCM56_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEG1110452.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(15608..16414)
                     /locus_tag="SCM56_001672"
     CDS             complement(15608..16414)
                     /locus_tag="SCM56_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1110453.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            16580..17536
                     /locus_tag="SCM56_001673"
     CDS             16580..17536
                     /locus_tag="SCM56_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1110454.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(17613..18131)
                     /locus_tag="SCM56_001674"
     CDS             complement(17613..18131)
                     /locus_tag="SCM56_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEG1110455.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(18219..18368)
                     /gene="rpmG"
                     /locus_tag="SCM56_001675"
     CDS             complement(18219..18368)
                     /gene="rpmG"
                     /locus_tag="SCM56_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEG1110456.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            18596..19042
                     /locus_tag="SCM56_001676"
     CDS             18596..19042
                     /locus_tag="SCM56_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEG1110457.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(19237..21132)
                     /gene="asnB"
                     /locus_tag="SCM56_001677"
     CDS             complement(19237..21132)
                     /gene="asnB"
                     /locus_tag="SCM56_001677"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEG1110458.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         6435 a         4548 c         3696 g         6726 t
ORIGIN      
        1 ttctaatgtt ggtatgttaa aactttacaa attctttaaa aaaagtgaca ataaatatag
       61 tgaacaataa ggagaggact ttttaatgcg tgttaataaa atcgattttc acttttcaaa
      121 actcagatat caacctgatt ttgaacattt ctcggatgaa gagttccaac agatcaagga
      181 acatactgtt cttcgttctt ataaaaaagg acagatcctt ttcgacgatg gtgatcccag
      241 acaacggttg tatatcttga ccaaaggact cgttcgttta gaacgttatg atgaaagtgc
      301 aacttattac tactatgact atgtgaatac aaataacctt tttcctatgt caggaatgtt
      361 tggagaagag acctacactt atacagctca agcgatgact gatattgaaa cattctacct
      421 tcctgtttcc tactatgaaa aattagcacg aaaaaatccc gaacagttga tctatatgat
      481 acaaaaaatt tctaaagtag ttgaaataca tgaattgcgt atccaaatcg gattgacctc
      541 tagcgccttt gaccgggtaa aacaaaattt attcattttg aaagaagaat tgggcgtacc
      601 aacaaaagat ggtcatatca ccattcctta tccgatcacc ttaaaagaat tagcagtgaa
      661 tagcggcacg acacgtgaaa ctgctggaca agtaatcaaa caactacgtg attccgggct
      721 gttagattat gacaaaaagc gttttacttt ctgtaagaca gctactactc cagctatgag
      781 agaataacgc gaacaaaaat aagcagtgac cctgataaat atcagggtca ctgcttattt
      841 tcctccctaa aacttctgaa agatccggca gttttagatc actggacaag actcaaagat
      901 tttctatgtg tgtttcagtt atgtaatatt cactgtttgc aaaaaatact gttagattaa
      961 ctagatgaag cgtcttgtcc tcatccgatc tagattattc gatattcctc gacaataaac
     1021 gattactcag tgagggcaat cagaggaacg gataatgata aataagctgt aaaaaatagc
     1081 aaaataacaa taatgattaa aagtatgtaa aaaatagggc tttgacaaaa acgtgtcaca
     1141 gccctatttc gattaaacgg tgtgtacctg tcaactcttc ggtcacaagt cataatgttc
     1201 ataaacatga ggagctgttt tttgaaattg ttccttgtga atcagagtta aacgacaggc
     1261 ttacgaactc ttgattaaac ggcgttcaaa agcttgcata cgcggtaata agaacagcca
     1321 ggtcaaaaac atgagaagcc gtttttcctg ggctgttctt attacttgat gcagggcagc
     1381 tttttcacaa cctctttatt cagataaaac caagcttgga tcagcattca actgatagct
     1441 agcaaaattt cttttttgca tttccacatg tgctgctgct ccaatcattg cagcattatc
     1501 tccacaaagt cgaagagggg gaatcactag ttctacctca gggagtttag ctgccaaagc
     1561 atcctgcaat ccttcacgca acccttgatt ggccgctaca cctccagcta taatcagttg
     1621 cttgactgga taattttgac aagctcgaag tgttttagta atcaatacat cgatcacact
     1681 tgcttgaaag ctagctgcta aatcattttt gtctagatct tctccacgct gttgtgcatt
     1741 atgcaccaaa ttgataaatg cactttttaa tccgctaaaa ctgaaatcat aattatcttc
     1801 atggatcatc gctcgcggaa aatggtaatt atcttttccc tgatgagcta gctgatcgat
     1861 ttcttttccg cttgggtaag aaagacctaa tactcttcca actttgtcat aagcttctcc
     1921 tgctgcgtca tctcttgtct ccccaatgat ttcatatgat ccatcttctt gcatatagac
     1981 taattctgta tgtcctccgc taacaagaag tgccatcaat ggaaactgga atggtttaac
     2041 cagacgggct gcatagatat gccctgccat atggtttaca ggaataagcg gcaattggtg
     2101 tgcccaagca aaagccttag ctgcagaaat accaatcagc aacgagccga caagtcctgg
     2161 tccataagtg acagcgactg ccgataaatc ttctgccgac acaccagctt ctaccagtgc
     2221 atcttccaag cataaggtga tttgttctac gtgatggcgg ctagctactt caggtactac
     2281 gccaccaaaa cgtttatgac tgtttatttg agaagcaacg atgttagata agatctctgt
     2341 tccatttcta acgaccgcta cactcgtttc atcacaactt gattcaatcg ccagaatcaa
     2401 ttcttcattc atttcatcgt ctccgttttt aactttctgc tcataatgat cgcatcttcc
     2461 gtcggattgc gataatatga tttccgttta cctaatatac gaaacttttc tgattcataa
     2521 agttttcttg ctgcttcatt agatagacgt acttctaaaa aaacatgcag cgaatctttt
     2581 atttgttccg taatcagtaa ttgtaacaaa tgccgtgcat gcccttttcc ttgttctgaa
     2641 actgcaacag caatatttgt gatctcgact tcatctatta ctttcgagta tccaataaag
     2701 ccacagatct ggttatcttc agctaaaagc agataatcag agtgaggctg ttggatgtct
     2761 gccaaaaact gcattttcgt ccaaggtgaa ccatgtggat aagcattttc gctaatcatc
     2821 catacgcatt ctgccaagta cgcttcatca aaatcttttt ttctgcaaat caactaatct
     2881 cactcatttt ctaaaagttt tatcatatcc aacgcatctt cttttgtttc agtgtaatat
     2941 ctttttttga tcgttgttgc ttgaaacccc aatttccgat aaagtctttg tgcatcttga
     3001 ttactcatac gcacttctaa tgacatcgtc tcacagcgat tcattatagc aaagttctcg
     3061 atttcgtcaa taagcagcga accgattcgt ttacgctgat atgccggtcg aacggcaata
     3121 ttcgtgatat ggcaatctaa accgaacgct cttgaaccga taaaaccaat cattcttcca
     3181 ttatcaaaga tacctagata tagatgagga agcggtgact tcaattctga tataaaggca
     3241 ctttttgtcc atggcaattc gccattatag acttctcgtt cgacatccag cagctctttg
     3301 atatcttctg tagtcaattc tcttagtacg taacgacgat cgatttgttc gactgttttt
     3361 tctgtataaa tcattttatc ttgaaaaaag cttctggcat acgctctaaa ttttttcaac
     3421 ataactttcg tctccaggtg tgtgcgtctc caaccatttt tcttctgctt ctactcgttt
     3481 taaatagcga ggaagaaaag cttgtttctc tgcaactggt tcaactaaag cacctaattg
     3541 agccacagtt acaccacttg gaatatccca tgctggaact gtattaatca tcgcactagg
     3601 caatatttcc tttatttcag agaagaattt gtgacaatca ctcccgacga aataaatagc
     3661 ttcattcagc ttttgtagtt gtgtgaacaa atcagaagca gaaatatgta catcttcaag
     3721 tacagtttct aatctatttt ctttccagcg gtatgcccca gcgtatacat tttttcttcg
     3781 agcatcaaac atgggcacaa taatattatc cacaccgatg caattagccg cgatggtttt
     3841 cagactagat actcctacta gctctttatc taatgtataa gcaatcgttt ttgctgtcgt
     3901 tacacctata cgaagccctg tataagaacc gggaccatca gaaaccgcga ttcggtcaat
     3961 gtcttttgga cttatttgaa gatctgcaaa caattggtcg attgcaggca tcagtgttac
     4021 actgtggttg cgtttgatat ttgtttgtat ttgtccaaga atttgttcgt cttctaccac
     4081 accgatcgcc aaagtttggt ttgcagtatc tattcctaat gtaatcatat atttgatcct
     4141 ttctgctatg acattctcgc cttccattgt agcatacttt tttattctga ggatgaaaat
     4201 accaaaggca aaactagtac aataatacat gattatctgc tggcgggcaa attttgcccc
     4261 ttcctgaaac acaaaaaagc tgcggtatca ctccattttc ccacagcttt tccatctttt
     4321 atttcgttga ttctcgaata atcaactctg tttccacaat aatctttctt gaaaccggtg
     4381 ctttggaatt acttaattga agaatcgtct ccactgctag ctctcccatc cattcggtat
     4441 aaacttttac agtagaaagc gagggcgtta catatttcgc tacactgaca tcattgaagc
     4501 ccatcacaga aatatcttct ggtaccacta aacctttttc ttgaatcgct ttcagtgcgc
     4561 caatcgctaa tgcatcatta gctgcaaaaa gcgcatcagg aacatgcgga ttcgttttta
     4621 aatattgctt cactgcttga tatcctgact cgacagaaaa atctgcttcg ataattggac
     4681 cttcgtcgta gcctttttct gccaaaactt gttgaaatgc cataattctg ggatctttta
     4741 gccgctgatg gtttttcttc gtatattctg ttccagcaag catagcgata tgttgcgctt
     4801 tatgggagac aaagtgttca atgacttttt gaacacttga cttaaagtcg acaactagag
     4861 agtcgattcc ttgagaagtg ccgtcagaat ctaccaaaag caaggcattt tgggatcctt
     4921 tcatttctaa taactgagct tcgtcgaatt ttcctagtgc gatcgttcca tctgccggaa
     4981 gatcactaat ggaatcccac gtttctttca ataaagtaac gtttgcttct tctgcctttt
     5041 tttcgattcc taggcgaata gataaataat aaagatcttc aagctcttcc gtatcgtcgt
     5101 accactggat caggcgaagc gtttgcttag tatttgctgc atttttacga tgtttcgtat
     5161 aatttaagcg ttctgctgct tcaaaaattt tttgctttgt ggctaagcca acggaaagat
     5221 caggatcata gttgagaact cttgaaacag tagcaggaga aactcctgcc aactgcgcaa
     5281 tgtctttaat tgttgccatt tgtctctcct ccttttttac gtttataaaa tcgcagtaaa
     5341 tcgttcaaac gctgcctgtc ctttttcatc tcgcttgaat actccagcgt cttccagtac
     5401 acgtgcaaaa acctgaccta cagatttttg tacgacatcg gtgaccgtat ctttcgtcaa
     5461 atcaggatgt tcttttttta attgttctgc ccacggacga tgatattcag ccatctggtt
     5521 ttcttctcct aataagaaat gctctacttc tactagttct ggtttcaaac gaggtggtaa
     5581 aacagcaagc cccattactt cgatcaaccc aatgttctcc tttttgatat gttggacatc
     5641 tttatgtgga tgaaaaatcc catcaggatg ttcttctgaa acattgttgt ctcgaaggac
     5701 aaggtccaac tcaaagtaat tgtcccttct tcgtgcaatt ggcgtaatcg tatggtgtgg
     5761 tgtaccatct gctgaaaaag ctctgataga tactgtttca tccgaatatg ctttccattt
     5821 ttccaatact gagttagctg cttcaataag caagtttttc tctgttgatt gcaaacggat
     5881 cacagacatc ggccatttca cgactcctgc gatgacttgc ggataatctt ctagtttgat
     5941 aagtttttct atcggtgctt ttgccatagg aaattcatga cgtcctgcct gataatgatc
     6001 atgagaaaga atcgatcctc caacaattgg taagtcagca ttcgaaccaa caaagtaatg
     6061 aggcaagact tcagtaatcc gaagcagtct agcaaatgtt tcctttgtga ttttcatcgg
     6121 gcggtgttgt tctgataaaa tgatcgaatg ttcattgtaa taagcataag gggaatattg
     6181 gaatccccag ctttcaccgt ctagattcat tcgaataatc cggtgattgg tgcgggctgg
     6241 atagttcaag cgacctctat atccttcgtt ttccatgcaa agcatgcagg taggatagtc
     6301 tacttttacc gcctcgcgtt ctgctgcgat ttgttttgga tctttttctg gcttagataa
     6361 attgattgtg atttccagtt caccatactc cgactctgcc ggaaatttga tgttttttgc
     6421 gatggctctt gttttgatgt agtcattttt cttacttaaa ttaaagaaat actcagttgc
     6481 ttcctctggg cttttcgaat agtgctgtgc aaagaatgca ttgacgactg aaggtggcgg
     6541 tgtgagaaaa tccattaatt gcgcttcaaa aatctcttgt tctgctggca attctgaaag
     6601 aacaccattt tctcttgcct tagcgacaag ctgatccaat aagtctacag attcttcctt
     6661 aactggtctt acttcaatgc tttctagagc atcttctcct accatcccta aaagacgatt
     6721 ctgtaaatat aagcggtcca tttccatcca tccgccagct tcgatcgcaa gtgtggcaaa
     6781 atcacaaatc gtttgactga ttgacatatc tcattcccct attcttcata accatttgga
     6841 tggctgacat gccaatccca tgctgttttg ataatcgctt tgatatctgt gtattcaggt
     6901 ttccagccca gaatctctct cgctttttca cttgaagcga ctaatcggct aggatctcca
     6961 gctcttcttg gtgctatttc tgctggaatt tcttttcctg tcacttctct agctgcttct
     7021 agcatttcct tgactgagta tcctttgctg ctgcctaaat tgaagaagtt gctttcattt
     7081 ccttctttca agtattccaa agctaaaata tgtgcagcaa tcaaatcttc tacttgaaca
     7141 taatctctga tacatgttcc atctggtgta tcataatcgt ctccataaac agccaaggct
     7201 tttctttgac caagagctac ttgtaaaatg attgggacaa gatgcgtttc tggtgtatga
     7261 tcttctccga tagatgcatc tgcttttgct ccggctacat tgaagtagcg caaagcgaca
     7321 tagcgcatgc catatgcttg gtcacaccac ttcatcattt tttccatcat caatttactt
     7381 tcaccatacg gatttttagg attagctggt gtatcttctg tgattggaga ctctttcggc
     7441 tctccataag ttgctgcagt agaagagaat acgattttat tcacgttgaa ctcgtgcatg
     7501 acttcaagta agatttgcat accgtatacg ttgttgttga aatacatcaa tggtttttcg
     7561 acagattctc ctactaaaga gcttgcagca aaatggatta ctccttcaat cggttccttt
     7621 tcaaatacgg aacgtagaaa ttctttatcc cggatgtctc cttcataaaa atgagcatct
     7681 ggatgtactg cttgtttgtg acctgtgagc aaattatcta ccacagctac ttgatacccc
     7741 ttttgaacta gctggtctac agcgtgcgaa ccgatataac cggctccgcc tagtactaaa
     7801 attgtcataa aaaacactcc tttgattagt aaaattgtgt ttgttgattc tatataattt
     7861 aaaaaacgaa gtttactttt atggtgaaaa caaacaaagt aaaatttcgt aaattcatat
     7921 ggtaaattta taataaaaca tttagtaaac attttcaagg gttttttgca aaggtttaca
     7981 aacaaacgta aaactagcct ttttttctct tttgttccat aatagatata acaaataata
     8041 aatgaggtgg attcaatgaa aaaatttgta tctggaatat tagtaggaag tctggcaaca
     8101 gccgctgccg ttgctggttt ggttgcttct gtgaaaaaaa cagtgatcga tccaattgac
     8161 gaaaaagaag caatgatcga agaaaaccgt aaaaaagcaa tgcgtaaacg cgtttctcgt
     8221 taataattat ttaaaaatac gcatgaagac tttctaaaaa acagataagt catcatgcgt
     8281 atttttattt gtcagtcaat ataagttgtg ctattttatc tgcctgtctc agattccatg
     8341 gatctgtata aggtctagct actgctgcag ttagccaatc cacaccttca acaggtattc
     8401 catagcaata aaagtcagac tggatttccc ctgaagcatc aatcacgcga ttcgtacctc
     8461 gctcaacatc tatagcttct gaatgataac ttcctgtatt atctgaaaat gaaaaagggc
     8521 gaacaagttt gttttccttc aaatttttta tgacaggatt acttgaacga gaaaagcttg
     8581 tttgtggcaa tcgagcttca atcaaatagc gactgctgat ttctttatct tgtagagaaa
     8641 taacatagcg gttgtgcttt tgctgaatat caaatggcgg ttcgatcaaa tgaaaaatac
     8701 ccgcatctat cagtgctgct aattctttcg tccgaatgat tggcggaccg atcgttaaaa
     8761 agctatttag atgagtaaac catccccata gtatttcttt gtattcctta gcagaaaaaa
     8821 cgtgccattc aatagcctgc cggattggat cacgccagtc ttttaatgca tcaaatgttg
     8881 aagcaaatgc tcctgtgcaa ttgccttttt ccgcttcttt tatctgccct tcaataaata
     8941 ttcggctagc ttctgaaaag ggctcattct gataggcaag aggagtttct aaatacgacc
     9001 aatcccatct ataagggatt agtgctggat gctttccgag acactcctct gaagagaggg
     9061 agagaaaatc atgctcaaaa tttttctttg gaatcggcaa gtgatattct tcgatgattt
     9121 tcttgtaata aaacaactca acgtcttttt tcagatagtc aaaaaatgtt tcgccagtca
     9181 aaaatcccga ctgatgccat tgctccaggt tttcttttgt tagtaaccgt ggccatgcca
     9241 ttgctccgcc ttgcttttga tttcttcctc gagggtaata agggagcccc cttcttgatc
     9301 cactgtatac aatcggttcc ttgcctgatg gatgatagac cagttttccc ttttcgttct
     9361 cgaagcgccc tcctcttgat actgtaaaca atccgacata atcaaaaaaa cttaagccta
     9421 gacctcttaa aaagacattt tctttaggcg gtatcttcga aagatctaca tctgcaggat
     9481 tagaaggaag ctgatacagt aaaacatttt tctttgcgta gtctacccac actctttctt
     9541 cttctgtctc atcattttcc cagtgaccgc ttgctaaaac aagctgatcg atagaatagc
     9601 tttgctgatc tgtctgtaaa agataaccat cttttctttt gcttactttt tttactaaag
     9661 aataaacaaa ctgaatagaa gctggaaatt cttcttgcaa ctttgagaaa aaccagcgtt
     9721 gatagagtcc gtataaacat cgagtagatt gctgatcttt ttccagacga ttgatttctt
     9781 ctataattag ttgcttattt ttggttaccg tctgttcgat ataatctgta gcaggtcctt
     9841 tactccattg aaacaagttg ggtccctgtg ttaccaatcc tccactggac aatgtttcat
     9901 ctgtaaaaag cgtcacttgt tgagaaacag aattcattaa taactccggt ggctgattca
     9961 accgccaaac tcgacctcca ggaccattgg gatcgaaaag aaaaagttca agagaatgtc
    10021 tgtcctgatt attatggaga atcctttctg ctacacttaa tcctctaggt ccggcaccaa
    10081 tgatccctat tttcatttgc atgcctccat ttcgataaat tttttcaata aaacagcatg
    10141 gttctgatgt tcattttttg caccataaac aaaagtaatg actggatgtt ctttcataag
    10201 ttgactgagt ttaaaaatag cttgcttctt ttcttcttcg aaaatcaatt cttgctggta
    10261 tttctttgca aatgaagcaa atcgttctgg tacatggtca aaagttttgc gtgcttcatt
    10321 actaggtgca atttctttca accacagatc taactgcgct ttttcttttg aaattcctcg
    10381 tggccagaat ctatccacga gtattctata gccatcctct ttttcagcat caaggtaaac
    10441 acgttttaat ttgatcatag ttgtttcctt ctttctacct tttcgcttag cttatatatg
    10501 tagataatag cacaaaataa aaaaaatgaa cggcacaact atccattgcg ccgttcatgt
    10561 gatcttggtc aaaatctaat gattgattcg gtttttaaag ctgtcataca atcatctgtt
    10621 ttatactttt ttacttttga tttttccaga ccatccgtca tatccaccct taagtaaata
    10681 aatctcttga taacctgctt tacgtaattt atttgctgta cggatgctca aacttttctt
    10741 ttgatcgtaa atatagacag gctggtcttt tcgaatagat cctaatgaat ctttcaaaac
    10801 agtgtaaggg atgtttcttg cacctaaaat atgcccagca tcaaacacat ctttttcgcg
    10861 aacatcgatt acttgtgctt ttctcattcc ttctcgaaat tcttcttctg tcaaagtcgt
    10921 tgctgaacgt ttggccatta ctcgtagata gacttcccaa aggatgattg caagcacaat
    10981 caatagcaaa atgatattaa ttacccataa aaccatgtaa tgcctccgtt tttttaagca
    11041 aatttcaagg ctaatgaggc agcaccaatg acaccagctt cattaccaag ctctgctaat
    11101 ttgatttttg ttgattctct tacttgcggg aaagtaaatt ctttaaaata tttctctaca
    11161 cggctacgta aaaattctcc tgctgccgat actcctccac ctaacacgat acttgacgga
    11221 tttaatgtat tccccaagtt tccgcaagct aaacctaagt agaagcatac gcgatcaaca
    11281 accatcaatg caaacggatc gtcttcttga gccatttcaa agatatcttt acttgttact
    11341 tcttctccgt tgtctagcat tgctttcagt tttgaatccc ctgcgtattc ttctgccaag
    11401 aaacgaccta ggcgtaccac acctgtagcg ctagatacag tttctaaaca tccgcgttta
    11461 ccgcaagtac attcgaaccc ttcaggatcc acagtgatat gtccgatttc tcctccagct
    11521 ccgctcacgc cgtgaatcaa atttccgcca gcaacgatac ctccgccaac acctgttcct
    11581 agagtaacga atacaacgtc tggatcgttt tctccagcac ctttccatcg ttcacctaaa
    11641 gcagcaacat tcgcgtcatt atcgatagcg aatttgattc ctgttcctgc ctcgatcagt
    11701 tttttcacaa attgaacttc tgtccagttt aagttatacg caccaataac cgttccagct
    11761 tcgctgtcca cgcttcctgg ggtacccatg ccgattccga taaagtcttc ggctttcata
    11821 ttatataaat tcaaacgatg attgattgat tcaatgattt caggaacgat atgcttacca
    11881 tcatccaaaa tattggtatc gatgctccat ttttgctgaa tctctccttc tggtgttaaa
    11941 attgcaaatt ttgctgttgt tccacctaaa tcaatcccaa taatcttctt gtccattaca
    12001 aatttccttt ctttcggctt tcttcaattc ggtgctctct ttttaatatg cgccacgctg
    12061 tcatgtatgt atcatgatca atcaaacggc tatcatacag attacgcaat tcgatagtca
    12121 ttaattcaat gtcatagatg cgtgcaccca catagataaa gatgccaaat tttttgaaca
    12181 gctgctgtac atcatataag ctctccatct ttaagcactt ccttcatatt atacaagtaa
    12241 accatatttt tttaaaccca gtaaaataca tattacaaaa agaaatacaa aaactagtgt
    12301 agaaagaatc ctttggtgta tcgaatagct tgtctgttgt ttcggtagac ccaaaacatt
    12361 ccctaacact aatccgccaa ttagcccacc gacatgtccc caaacatcta cagaggtatc
    12421 cagaagacca aaaacaaatg tcaacacaat aaataaaaga aaatgtctga ctagctgctg
    12481 gatcgctggg ttatgtttaa aatgaaaccc taagataaac aacgcaccga acaaaccgaa
    12541 tatcgaagta cttgcaccac cagacagaac attcgcttcg ttaaaagcaa agcttgctgt
    12601 atttcccatt atcccgctga acatataaat caagaaaaaa cgccaatggc catagattgc
    12661 ctcgatttgc tgccccataa aatacagtat cagtgagttc attacgaaat gcatcagtcc
    12721 aaaatggatg aagattggtg tcactaaacg ccaccattca ttgaagtgaa caagataagg
    12781 accatacatt ccggcataat aagggatctc aagtcgcgga aataattcca tcatgaggaa
    12841 gaccgctgtc tgtatcccta aaaaagcata ggtccataac ggtcgtctta accatgcacg
    12901 caattttatt tgctgttgat aattcatgaa aacctcccat ttcattttag tcaataaaca
    12961 attgttggat cttctgatca aagctctccg gttgccattg ttcattcaat tgttcacgaa
    13021 aaacaaggct acatgttttt ttatgaaaat tatttaaata ccggtcgtaa taaccaccgc
    13081 caaaaccgat tcgataaccg gctctattgt agataatacc tggtacaatg aggagatcga
    13141 tcatctccgg caaatagagc ttctctgatt gtggctcaag aacaccaaaa ctcgaacgta
    13201 caagaacgga ctgttcatcc atttcataaa acgccatctg acgtttcggc aaagttttag
    13261 gtacaactat tttcttttgt tctttttctg ctcgttggat aaccggctgt gtatccagtt
    13321 caaattcatt cgataaagtc gtccctatca cttttgctga cttccagtac tcgctggcga
    13381 atagctgttt atagattcgc tgttcttttt cctttttctt gccacttttg gccagatttt
    13441 tcagcttttc gattcctttc cggcgtaatc ttactttcaa ttggtccaaa aactcaccct
    13501 tccttctctc ataaaatggt ataggaaaaa aagaaaaata gcaatcgaac tttcaaataa
    13561 atgcataaat agaataaaaa gaagacctag aaatgattgg ccttcctttt ttaatgaatt
    13621 tcaactgttc agcgcgtgtt tttgtctgat aaaaagtgca tacagaaaat atggcgcacc
    13681 aaaaagggca atcaaaattc ctgtaggtac ttccccactt ggtagaatca ctctagcaac
    13741 catatctgca ctaagcagaa aaatggaacc gaacaaaccg ctcagaacaa aggtccagtt
    13801 attttcttta cgtacgacta gcttcgcagc atgaggagct agtagtccta caaaagataa
    13861 actacctgtc accgctacac taccgcttgc caaagcaaca gctaacaaca aaaggatttt
    13921 ttgactttta gccacgttaa ggcctaatcc ttgcgcgcgt tcttgaccaa atgatagcaa
    13981 tacaatcctc tgtgcataaa gtcctcctag cggaattaag ataaccgccc atggaagcaa
    14041 tgcccaaaca taaggccagc tcgcacccca gattgatccc gctaaccatg cattcacaaa
    14101 tccataattt tccttagata attggatcgt gcatagcaat gttaatgcag aaaggccggc
    14161 attaacggca ataccggaca ataagattcg attcataccc aaaaagccat ttcgatcata
    14221 ggccgcaaga tagacacagc aagcagctaa aacaccacct agaaaggcta caactagcaa
    14281 tagaaacggc agcgaatagt gcgaaaagaa acctaaataa agcatcacta aaaatcctgc
    14341 tccggcatta atgcccaata tactagaatc tgccaattca tttcttgtta ctccttgcaa
    14401 gatataacca gaaacgccaa gacaagctcc tgctaataaa ctgatcagta atcgaggaac
    14461 tcgaaattct ctaacaatca gcgccaaaga agatgattct ttccctatga acgtggcaaa
    14521 aagctgatcc aggtaaataa aaggcgtacc aatcattaaa ctacctatag acaggatgac
    14581 aagtacaaaa agcaagaaat agaacgtcca atatattttt ttcaccgttg ttccctcctt
    14641 acttgtatca gtaaaaacgg aacgccaatc aatgcaatga ctaatccaaa aggtgtttca
    14701 aaagggggat tgatcatcct cgctgtaaaa tccgccaaca taaccaatcc accgccaact
    14761 aaaaaagaga caggtagaac ataacgatag tccgttccaa ccattccgcg aacaactggc
    14821 ggaaccaaca gcccaataaa tgaaacaggt ccgactaacg aaacggcaat ggctgctaac
    14881 actgcaacta gacagaaaac aatcaaccgc gttcgtttag gatgacttcc aagagctaat
    14941 gcatgtgttt cactcaaatg aatcaaagat attgcttttc ctaatgaaaa agaaaccaaa
    15001 acagcaagta tatataaagg aaacaatact gctaattgtt tccatgtgat attggcagct
    15061 ccgcctacaa accaaaaagc aagatcctgc tgcagattaa agaattgggt aagtgcttgg
    15121 ctgatagcag taaaaaaaga actaatagct acgccagcta aaatcagctg taaaggattc
    15181 atgcccattt tcgaaaactg caaaaaacca aatacgagca aagttacagc aaaagaacct
    15241 gccaatgaca ttagaaacac agaaaaatca gaagctttag gccaaaaagc aaaactaagg
    15301 gccattgcca acgaagcacc cgcgttgatt cctagtaagc ctgaatctgc taaaggattt
    15361 cttgtcaccc cttgcatcaa tgcaccgctc aatgcaaaac aactccctac aagaaatgct
    15421 cccgccacac gaggcaaacg aatatttcgt atgatttggt gactttgttt tgatctatca
    15481 aaatcaaaca acgcctgcca aaaatctgct ttttccacag tcgctgcgcc atttgaaaga
    15541 gcaacaaaaa aaataatcaa aagaacaaaa cagaccaaaa gcaggagtct atttttcttc
    15601 atagtcctca taattgatcc tcctgctctt ttggcaacaa atcataggtt agaaaaactg
    15661 gtttatttgt tcttggaagc gtgacaatcg ttgcatcaat ctcaaatatt tttttcatac
    15721 actcttttgt gatcacttct tcaggtgtac cttctgtgat caacttaccg tttttcattg
    15781 ctaataaata atctgaaaaa cgtgaagcat gattaatgtc atgaatcgac atcaagatcg
    15841 ttgtttgttt catttgattg atttcagtca ataactgtag gatctctaat tggtgtgccg
    15901 gatctaaaaa tgttgtcggt tcatccaaga tcaagatatc tgtttcctga gcaagcgcca
    15961 tagcgatcca gacacgctgg gtttgtccac cagacagcgt tgcgatatcc cgcatacgaa
    16021 atccagctag acctgtagca tccaacgccc actcaattgc ttcatgatct tgcgtgtcca
    16081 tgccactgaa cagtttttga taagggtatc gtccgtaaga tactacttct tctactgtca
    16141 tacctgtagg gtgttcacta gtttgtgcta ataatgcaat tttttttgca agttcttttg
    16201 aactgtatgt agagatatca gagttattga caatgatctt tcctgatttc acaggtagta
    16261 tacgggtaaa acttttcaat aaagtagatt ttccacttcc atttggccca atcaaactag
    16321 tgattttccc ttcaggaatc gtaacagaca tgttttcaat gatttttttg ttttcgtagc
    16381 cggctgcaat gtcagctgct cttaatctcg tcatttcacc catcctttcg ctgaaaatca
    16441 ttctcaatta tgacgctgtt tcaacatttc gtcaagcgct actttcatat ctatagccaa
    16501 atgaagcggt tgacagcgtt cgaaatatca ggaataataa ccaatgagaa agattctcaa
    16561 ttatgttagg aggaaagaaa tgaaattaaa aacaaaagta ttcacattat ctagcatatt
    16621 gctgctggct ggagtactga gcgcttgctc cccctcttca gataaagaaa cagctacaac
    16681 tgccaattcg acgacttcat ccaatagtac tcatacagtc acagatacac ttggacataa
    16741 agtagagatt ccaaacgcgc ccaaacggat catcggcagt tacttagaag actatctgat
    16801 tgcactaggg gaaaaaccta tcgcccagtg gactgttggc agtggttcca ttcaagatta
    16861 tctgcaagat gatttaaagg atgtcccaac gatttcttat gaccttcctt atgagaaagt
    16921 gttaagcttt gagcctgatt tgctgttgat ttcttcttcc gctactgtcg aaggcgggaa
    16981 atacgaacaa tatagcaaaa tcgcgccaac ttatgttgta aaaaacggaa cagatgtcac
    17041 atgggaagaa caactaaaag atgtcggcaa agctttggca aaagaaaaag aggcagaaaa
    17101 aatcatttcc gattatcaaa aatccatcaa gaaaactcga gaagaactag cagataaaat
    17161 cgagaacaag acagctgcag tgttgtgggt gaccaacaac tcagccttta tggtcagcaa
    17221 aaatcggtca agtggaagaa tcgtatacga cgatctggaa tttggtgttc ctgatctagt
    17281 ggaagaggtc tccaaaaaag ccacttctga ctggtctgct gtttcctctg aaaagctagc
    17341 tgaattagat gctgactaca tcatccttgt gaacagtgac aaagatgctg ccatgttcaa
    17401 tgaaccaagc tggaagaatc taaaagcagt gacagacaat catgtactgc aattcggacc
    17461 tgaatctagt tggctatata acggacctat tgccagtcag gaaatggtca atgatatcaa
    17521 agaaaaacta aattgatttt aactaaaaaa agcatcttca cgattagtcg tgagatgctt
    17581 tttttcttat taacaaacta gacgcataga ttttattctt cttttgaagc aaccctattt
    17641 tttactcttt cccatttttg aaaacgatgg gcatatagat tttcttgatc cgttatcccc
    17701 tcatactcct ctataagggc gaagtctgaa aaatcaatat tcccaatata cgtatcgcct
    17761 tcaaacgtgc tatcgatcaa tgttcgccag atccgaccag tgtaaggaag aagttcttga
    17821 aaaatccgac taccacccga aacatagata tcttctgaga tcgtttgact atacgacaaa
    17881 agttcatcga tcgaatgcat tacttccgca ttttctttcg tgcgaaaatt tggttgcgtt
    17941 gtcaacacga tgatatggcg ataaggaagt gaaagatccc ccattccttc ataggtcttt
    18001 cttcccatca ctaaaattcg atccattgta tgttcgcgaa agaaacgcat atcatttggc
    18061 aatcgccatg gtaacaaacc atttttgccg atcagaccat ttttatcttg tgcccacatg
    18121 gaaataaaca tattatccgc tccttatgat tttagatgtt ttgagataaa aaaagtctca
    18181 attctttgac cagcaaggaa ttgagaactt taaaaacatt attttgtttc acggtgcaaa
    18241 gtaactttgc gttcgcgtgg gcaatatttt tgtttttcca aacgatcagg attgttacgt
    18301 ttgtttttgc ttgttagata gttacgttct ttacaagaag tacactctaa agtgatgttt
    18361 acgcgcatgt ttttccctcc catttttaca agaatcctct aagatgactc tcagacttga
    18421 ttatcttacc atgatttttt ctagattgct agtgtttttc acgattttgt taagtaaaac
    18481 tacttgacga taaatacaac ggttttactt ctcaaacaaa atctttttct ttttgtataa
    18541 atacccttac acttgtttat atagattatg ttttttaata aagggggata ctaatatgcc
    18601 atggaacatg aaggactttc ctgcatcaat gaaaaactta gataaattga caagaaaaaa
    18661 agcaatcgac atcgcaaatg cattattaga tgaagggtac cctgataacc gtgcgatccc
    18721 aattgccatt gatcaagcaa aaaaatggta tgaagatgct agtgaatcag aaaaacgaac
    18781 tttcgaaaaa gaaaaaaatc caagcaagac ggatgaacac gatacgaatc ctcgtgctgg
    18841 aaaactgctt gattcagatg tgatcgtaga atatgaggaa gaacaatgga tcgtgaaatc
    18901 taaaggggca aaaaaggcaa gcaaccattt tgatacaaaa aaagaagcaa tcgaaaaagg
    18961 aaagcaagta gcacaaaaca aagaatcaac tttgttcatt tataaaaaag atgggacaaa
    19021 agaaaaagaa atcgattatt gaacatatgt aaaggggttg tgacgaaaat cgtgtcacaa
    19081 cccccttatg ttcgaaacaa tggtgttcct ctgccaactc cgaaccataa gtcataatat
    19141 ctataaaacg ggaagagctg ttttttgaaa ttgtttctta tcactcagtg ttaaacggta
    19201 ggagaaaacc tcttttttat tgatatttga ttttcatcaa acagtcacat agtcgattgc
    19261 ttctgcttgc ggaatcgttt catctacaaa gtagacttta taccatgtca agaaactgaa
    19321 gattgtccag atttttcttt gctcttcttt acgtccttcg tgatggtcat ctagcaactt
    19381 catgattttt tcttgatcaa aaaattcttt tgcaaaatct gcttcgaata atgctttgac
    19441 ttgttcgtag ccgtgttctt ctttcaacca agctttgatt ggcacaggga atcctaattt
    19501 cacacgattc gaccattctt ccggaagatg tttgttcgca gcttgtctga aaatatcttt
    19561 tgtgttgttt tgattcaata aaaacttact tggtatttgt tgagccaatt tcatgacttc
    19621 gatatccaat aaaggtactc ggacttccaa agaacttgcc atagaaagtt tatctgcctt
    19681 taacaagata tcttttggca tccattgatg aagatcgaca tattgcatct tattgacttc
    19741 atctttgatt ccttctgttt tctcgtaatg aacagtcatg atttcatcca cagatggtgc
    19801 tgtttgatat tttggtgtta acacttctga tgcttctgat tcttcgaaca ctcgagcatg
    19861 accgataaaa tattcttttg caggagcaag tgattcatat aaatggatcc gtccatggaa
    19921 gtttttcatt tttccgattt tacgtcctaa attatagcgt gttccttttg gtaatttttt
    19981 caatccttga gcaatcacac ggatgaattt tgaattcgta tggaatccat acgtttggta
    20041 tcccgcaaac agttcatccg ccccttctcc agattggact acccgcacac tttgtgaagc
    20101 aagatttgct aaaaagtaca aaggtacgca agaaggattt gaatcaggtt catctaagtg
    20161 atactggatc aaagggaatg ctttgaacga catgtctccg tcgatcactg ctgctgtatt
    20221 atctaagtcc aatagttcgg ttagttttct ggcttcaatc gcttcattat aggttttatc
    20281 atcaaacccg attgagaaag aatgatctgg tttcaataca gaggttacat aactggaatc
    20341 aaccccgctg gataagaaag aaccaacttc tacatcactg accgtatgtg cttcaataga
    20401 agcttgaacg gtttcgtcga ttttttcgat ccattcttgt cgggaatgcg tttcttttgt
    20461 ttcaaaatct gcatcccaat attgctgaat gtctattttt ccatctttat acgtataata
    20521 atggccttca ggcaaacgat agacatcttt gaagaatgtt tcgccgttta atggagaata
    20581 ttggaaagtc atataaggtt tcaacgcttc tttgttcaat tctttgttaa aatcaggatg
    20641 aggcagaaaa cttttgattt ctgaaccaaa cataaatgtt ccgttcattt ctgcataata
    20701 atatggtttg atcccaaagt ggtctcttgc accgaataac tcattctttt cattatccca
    20761 aatagcaaaa gcaaacattc cgcggatctt ttgcaataat tctgttcccc attcttcgta
    20821 accatgcaaa aggacttctg tatctgcatg tgtttgaaaa acgtggccag ctgcaatcaa
    20881 ttcttctctc aaaggctggt aattatagat ttcgccatta aaaataatga tctttgtccg
    20941 atcttcattg taaataggct gagtaccgcc ctctaagtca ataatactta atcttctgaa
    21001 tcctaaagca acatgtttat cgatatattc tcctgaacta ttaggtccac ggtgaacgat
    21061 tcgatccatc attgtattaa taattgtttt tttgttgtcc ttgtcgttaa caaaacctac
    21121 aataccgcac atttaacttt cacatcctaa tataaaatta ctgtttattt cattcatatg
    21181 catcttatca tagaataaat agcggacgaa tattttctct cttacaattt cgtcacgtga
    21241 aaacaaaaga tgaaagtttc tgtttttaca gaaaataaag tcccaatatc taatttacca
    21301 tgttttcttt tttgaggcta ccttattctc ttcgtgttaa aaaaatatta gtggatgcta
    21361 tttccaaaaa aaccagcaaa aaaagacacg acctaaagat cgtgt
//
LOCUS       DARBDV010000049        21281 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000049 DARBDV010000000
VERSION     DARBDV010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.049"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(34..1296)
                     /locus_tag="SCM56_001678"
     CDS             complement(34..1296)
                     /locus_tag="SCM56_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1110459.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            complement(1289..2188)
                     /locus_tag="SCM56_001679"
     CDS             complement(1289..2188)
                     /locus_tag="SCM56_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1110460.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            complement(2333..4111)
                     /gene="uvrC"
                     /locus_tag="SCM56_001680"
     CDS             complement(2333..4111)
                     /gene="uvrC"
                     /locus_tag="SCM56_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEG1110461.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIERLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            complement(4293..4607)
                     /gene="trxA"
                     /locus_tag="SCM56_001681"
     CDS             complement(4293..4607)
                     /gene="trxA"
                     /locus_tag="SCM56_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEG1110462.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            complement(4694..7054)
                     /locus_tag="SCM56_001682"
     CDS             complement(4694..7054)
                     /locus_tag="SCM56_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEG1110463.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            complement(7169..7716)
                     /locus_tag="SCM56_001683"
                     /pseudo
     CDS             complement(7169..7716)
                     /locus_tag="SCM56_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            complement(7774..8211)
                     /gene="zapA"
                     /locus_tag="SCM56_001684"
     CDS             complement(7774..8211)
                     /gene="zapA"
                     /locus_tag="SCM56_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEG1110464.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            8413..9330
                     /locus_tag="SCM56_001685"
     CDS             8413..9330
                     /locus_tag="SCM56_001685"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEG1110465.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            complement(9586..10497)
                     /locus_tag="SCM56_001686"
     CDS             complement(9586..10497)
                     /locus_tag="SCM56_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEG1110466.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(10900..11478)
                     /locus_tag="SCM56_001687"
     CDS             complement(10900..11478)
                     /locus_tag="SCM56_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1110467.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(11498..12166)
                     /locus_tag="SCM56_001688"
     CDS             complement(11498..12166)
                     /locus_tag="SCM56_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1110468.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(12181..13251)
                     /locus_tag="SCM56_001689"
     CDS             complement(12181..13251)
                     /locus_tag="SCM56_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1110469.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(13422..14786)
                     /locus_tag="SCM56_001690"
     CDS             complement(13422..14786)
                     /locus_tag="SCM56_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEG1110470.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(14783..16342)
                     /locus_tag="SCM56_001691"
     CDS             complement(14783..16342)
                     /locus_tag="SCM56_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1110471.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(16398..18878)
                     /locus_tag="SCM56_001692"
     CDS             complement(16398..18878)
                     /locus_tag="SCM56_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110472.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(18862..19197)
                     /locus_tag="SCM56_001693"
     CDS             complement(18862..19197)
                     /locus_tag="SCM56_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110473.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(19211..20245)
                     /locus_tag="SCM56_001694"
     CDS             complement(19211..20245)
                     /locus_tag="SCM56_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEG1110474.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(20335..21015)
                     /locus_tag="SCM56_001695"
     CDS             complement(20335..21015)
                     /locus_tag="SCM56_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEG1110475.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
BASE COUNT         6068 a         4451 c         3626 g         7136 t
ORIGIN      
        1 aatgtccagc ttccttctat atttttctat attttatgct ttttttcgtt catttctcat
       61 aattgaaatc gattgtttca atgaactcca taatccgcct tcgctataca cgagtacatt
      121 cgatttgtac atgtttgcag acaagatcat caataagaat gttacggcta caagtaccaa
      181 caaggaaatc aatgctccac ccataccgac tgtctcattt gctaagcgaa ccggcataat
      241 atatgaagat aagaatggaa tatacgaagt gatccgaata acgatattgt ttggatctga
      301 agctcctagt atcaatccaa gcatatatcc gcctagagat agataagtca ctggcaaaat
      361 tgcttttgct gtatcttctg ctttgttgac caaagagcca cataaagctg ctaaaacaga
      421 aaaaatcaaa atgccaaaga tcatgaatag aagagaatac cacaagaatg gtccaatcaa
      481 gttatccaat gagataccgt ctaatatatt tttgaccatt tccatatctt tgaattgact
      541 gtatcctata ccaaaaataa taccgtataa tgccatttgc gttaatgcaa caagtaaaac
      601 acctgttagt tttccgtaaa aatgcttttg tgcagtcgta ctggacagga tcacttccat
      661 gatccgcgtg cctttttcag aagcgatctc ttgtgcaatg atctgcgcat acgtcaaaat
      721 aataatgaac aagacaatcg ttgctacata acttacgaca tattggactg tcgagttatc
      781 ttctccaact gtcatttttc cttttgaatc aaaattgact ttttgtcgag tcaaacttgc
      841 ttgttggctc aagctagcta cttcttctgg tgaaatgcct aattgacttg ctcgaagcat
      901 cgattgcaag ccagtcagtt gttgttgtat cagtagttgt gtactttgtc ctaatgaatt
      961 ttcgctataa agcatcccat ttatcttatc attctcagtt gagactacta aatacgcatc
     1021 gattttttct tccgaaagct ctttttgtgc tttttcttca gaatcaactg ctttgaactc
     1081 aaaatcatct gttttgactt ggctcatttg ctgactgatt tgctcattat cagccaatac
     1141 gccgatttta ttgacatcac tattttgttg agcaaagttt cctgctacat aaatgatacc
     1201 cagcatgata aaagggacta atatcatgat caagaaagaa atcgacttaa cattttttaa
     1261 atagacatct ttggtaataa tccaaaactt acgcatcgat ttcacctgct ttcaatttga
     1321 aaatctcttc caaagttggc ggttgctggt taaacatagg aatgtagccg aactgagtcg
     1381 cacgagtaaa gatttctttc ccagcttcag gatcttctaa ggtaatctct aacgtattgt
     1441 cttcatgaag gatcactttt ttcacgcctt cgatttcttc tacttcctgt tggcttaaac
     1501 cagattccaa gaataacttc gtacggccaa atgattcacg aatctcatgt acttttccat
     1561 tcaaaaccat ttttccgttt cgcaacatga tcaaatgatc gcagattttt tctacattat
     1621 ccatattgtg gctagaaaaa atgacacatg aaccatgttt tttcaattcc aatattccat
     1681 cttttagcaa ttcagcattt accggatcta acccgctgaa tggctcatcc aaaatgatca
     1741 atttaggttc atggattaac gtggcgatca gctggacttt ttgctgattt cctttagaca
     1801 aagattttac tttatccgtc tttttccctt ttacctgaaa tttttccatc caaaaatcga
     1861 tttttggttc aatctctttt cttgatttcc ctctaagttc tgcaaaataa agcagttggt
     1921 cttgaatcgt tactttcgga taaagtcccc gttcctccgg aagataaccg atgacatcat
     1981 aatctttctc cccaagatga tggccattcc ataacacttc tccttggtct tgtgtcaaaa
     2041 aatccaagat caaacgaaaa gtcgttgtct tcccagcccc gttttggcca attagcccca
     2101 tgatttttcc atcggggatt tggaaagaga catgatcgac agctgtgtag ttcccaaaag
     2161 ttttaacaag atccttgact tctaacattt cttcactcct tcttcaagaa atcacatata
     2221 tccttagaca ccagacgtaa gcgatttatt atttaccaac aaaacgtaaa cgctcttttt
     2281 gttgtacata aaatgatagg acactttact ttctttgtaa agtgcccttt cctcattcct
     2341 gatgtaattt atcaaatacg ttttgtgcga catttttcgg cagtccgatt tcttgtaatt
     2401 cttctgtcgt ggctgctgtg atatttttca acgatttgaa ctctttaagc agcatttttt
     2461 ttcgttttgg tcctaacctt tcaatttcat ctaatcggga agcaaaactg tttttactgc
     2521 gtagctgccg atggaaagtg attgcgaatc gatggacttc atcttggatt cgctggagta
     2581 agaaaaactc tggtgaattt cgctcaagcg gtactacagc caaatctata ccgaatagca
     2641 actcatttgt tttatgttta tcgttttttg ccatgccagc aataggaata tcgattccta
     2701 attgattttc taagacatct tttgccacat ctacttgtcc ttttcctcca tcgatcaaga
     2761 tcaaatcagg taaaggcaac ccttcgcgaa tcactcgtga atatctgcga tagatgactt
     2821 ctctcattga tgcataatca tccggtccgt tgactgtttt tattttatat ttccgatatt
     2881 cttttcttgc cggttttcca tcaatgaata cgaccatcgc agccacagga ttcgtcccca
     2941 tgatattaga gttatcaaaa gactcgatac gtattggggt cgggatattc atcgcgtttc
     3001 ctaaacgttc aactgctccg atcgtccgtt cctgttttct ggcaatcaga tcaaaccgtt
     3061 cattcaaagc aactgaggca ttcttattcg ccaattcaac taattttttc ttttctcctc
     3121 gttttggttg cagcactttc gtagctaaca aagcttctac actgggcttg tcgatcgtat
     3181 caggaatcaa gacttcttta ggaatgaaat gctcattttc ctgataaaac tgaccgaggt
     3241 aagtcaagaa atcttcttct gcttcgttat agaaaggaaa cattgaaaca tctcgttcaa
     3301 ttagctttcc ttgtcttacg aaaaacactt ggacacacat ccatcctttg tcaatcgcat
     3361 aaccaaatac atctcgatcc agtaaatcag tatttgtcat tttttggcga gtcatgattg
     3421 tctcaatggc acggatctgg tctcggtatt ctgcagcttt ttcaaactcc atgttttcag
     3481 cagctttttc cattttctga tggatctctg actcgatttt ttcgtgtcct ccgtttaaaa
     3541 actttttcac ttcatcaacg attttcgtat agactttcgg atctacgtcg aaataataag
     3601 gacacagaca ttgtcccaaa tgatagtaaa gacaaggctc cttcgtctga ctaggtttgc
     3661 attttctcag aggaaataat cgatcgagga tcttctttgt ctcattcgcc gcaccgacat
     3721 cgggatatgg tccaaaatac agtgctttgt cttttagtac tttacgagta atcaccaaac
     3781 ggggatattt ttcattcgtt atcttaagga aaggataggt tttatcatcc tttaacataa
     3841 tgttgtattt aggatcattt ttctttatca gattgatctc taagagcaat gcttcgatat
     3901 tcgattctgt aacgatgtat tcaaagtctt cgatttcact aacaagccgt tccgttttcg
     3961 tattatggct gccagtaaaa tatgagcgga cacgattttt taatattttg gctttgccga
     4021 cataaatgat cgttccattt ttatctttca tcaagtaaca gccgggctga tcaggaagta
     4081 aagctaattt gtttttgatt cgttcgttca tctggtactc cccttcatat tcaattaaag
     4141 ttattatagc atgtctgtca aaaaaacaat ccagcctgac aacgtaatta tactcaaaaa
     4201 atattaattt gaataacctt aactcaaccg caaaaaaacc gaggacggaa ctggattcag
     4261 tcccaccctc agcttatttt tgctgtaagt ttttataaat attgatcgat catttgacgt
     4321 agttgttctt ttgaatgaac accaacagct ttttctacta cttgaccatc ttttttcaac
     4381 attaatgttg gaatgctcat gataccgaat tgttgaggag tttctggatt ttcatccaca
     4441 tccattttag caattctgaa ttcttcttca tcatattctt gttccaactg atccaaaatc
     4501 ggtgcttgca tacgacatgg accacaccaa gttgcccaaa aatcaatcaa tacaagtcct
     4561 ttatctgttt cttcattaaa tgtcttatct gtaattactt gtgacatatc aaagacctcc
     4621 atttttaaaa tctatgatta gtatagcatt tctatacttg gatagcgaac catctgctta
     4681 cctttagtaa gctttactta aatttaacaa ttgtcgcacc atttccgcct tggtttgccg
     4741 gtgcaaactc aaagcttttg acgctccgat ggtttttaag ataatcagta attcccttgc
     4801 gaagtgcgcc tgttcctttt ccgtggacaa tagtcacttg aggataacca gctagaatgg
     4861 cagaatccaa atattgatct acctcactta gcgcttcttc gtatcgtttt cctcgaaggt
     4921 ccaattgatt cggcacgtga ctactagatt cagatcgtac agcatggata actcgttgtg
     4981 tcggctcttt ttgaggagca acaggcgtca gctcgtcttc ggacacattc atttttaata
     5041 taccgatttc tacttgccac tgattcccgt ttttacgtaa aagcgtccct ctttggccgt
     5101 aagtcgtaac caacacttcg tctcccggtt tcaacgtttt ttgttctttc gccttcttca
     5161 atactttatt tttcttcaaa gtttcttctt gatgtagatt tgctaattgg gtctttgcat
     5221 cgatcaactg atgctctttc acatttcctt gcccaatctg ctgttgcatt ttacgaatat
     5281 cagcaatgat tttttctgct ttttcttccg cctctgacac gacttcgttt gcttttttct
     5341 ttgctttttc catttctttt tcacgttctt caaagaaata gctgtaagct tctttcaaat
     5401 catcgtgaag ttcttgtgcc tcagaaacaa aatgacgcat ttccagatat tcggtctctg
     5461 ccattttccg gcgattctct aaatctgtga tcatttcgtt taaatcttgg ctctcatcat
     5521 tcatcaactg cttcgcttca tcaataactt ctgtatcaag acctaatcgg ctagagattt
     5581 caaacgcatt gcttcgtcca ggaacgccaa tcagcaaacg atacgttgga cttagtgtat
     5641 ccacatcgaa ctccatgctg gcattaatcg tatttgcccg attgtatcca taaactttca
     5701 attcaggata atgcgttgtt gccatcacat aagcagactt tttaccaaga tcatctaaga
     5761 ttgcgattgc tagagcagca ccttcttgcg gatctgttcc tgcaccgagc tcgtcaaata
     5821 ataccagact tttttcattg acttttgata aaatatctac agtgttcgtc atatgagaag
     5881 aaaaggtcga tagactttgc tcaatcgact gttcgtctcc tatatccgca aatacttcct
     5941 caaaaatacc gatttgactt tcttcacctg caggaatcgg taatcctgct tgccccatca
     6001 gttgtaacag acctaaagtc ttcaacgtga tcgttttccc acctgtgtta ggtcctgtaa
     6061 tgacgatggc ttgatagtct tttccgattg tgatgtcatt agaaactact ttttcctgat
     6121 cgatcagtgg atgtcttgct tgtttcaaga cgacatgatt gtcttcactg attcccggca
     6181 cgatggcttt tacttcttta ccaaatcgtg cttttgcatt catcagatcc attttaccaa
     6241 tcacataagc attgtggatg atttcttgac gatggggaac aagttcagca gaaagttcac
     6301 ttaaaatacg ttgaatctca tttcgttcag cgatttggta ttgacgcaaa cgattattca
     6361 aatccaccac ttgttttggt tcaataaata aagtctgtcc tgaagcactt tgatcgtgga
     6421 ctacaccgcc aaatacaccg cggtattctt gtttgactgg gataacgtag cggtcatttc
     6481 gcatggtgat gatcgcatca cttaaatact ttgcattttt cccacggacg atcccatcta
     6541 actgttcacg aacagcttgt tcacttcgcc gaatgttctg tcttatgctc tttaactcag
     6601 gagaagcgtc atcagtcacg cgtccgtctt catcgattgc ttcctttaaa cggcggctca
     6661 aaacagggat cgtgactaac tgatctgccc acatatacaa acgtgcaaat tcgatttcac
     6721 tgtcagaaag atcatcaatg aaacgattca attcgcttgt cgtagacaat actctggaaa
     6781 cttgtgcaag ctccactcca ttcaagtctg ctccgatctc gatccgtttc atatggggac
     6841 ggatattttc aagtttaggt atagggatcc cgcctcgcag acgttgcact ttcaatccat
     6901 cctcagtttc ttgaagccag tttgtgatcg tttgtttctc actcactggt accaattctg
     6961 ccagttcttc ttttccttgt gcagtcacta caaattgtct gaccatctgt ttgacttttt
     7021 caaattccaa tgtttcaagg attcggttat tcattttatc accttatctt tcactatgaa
     7081 aaaagagctg tgccggacat acgctacgca gccttcttaa ataaactact ctgtacaaaa
     7141 aacgaaatat tttccagcac tattttcctt aattcaaaat attcgttacc caaagctcat
     7201 acatcttgtt ggtaagaaat ggcgttctct caacgataaa acgagcgaaa ctatgctcgc
     7261 cgaattgatt ttggaccaca ctaatggaac aaaggcaagt aactgcaaaa tcaaaaatat
     7321 agtaacgtaa gccatgatta gtgacaggat gcctcctgca atccagtcta cctgtctcaa
     7381 gatcggcaca tacgtcaatc catggacaaa aacccccaca aatcgggtaa gcagccagcc
     7441 aattagcaaa ataatcagaa aagcagttcc tgcataaaaa gcttcatcta ggtgaaaagc
     7501 aaatacctga tcaaaaaatg ctaactttga tgttggggtg acagctgggt atggaatgta
     7561 taattccaaa tgacttgcta atggtttgta aaaatgctgg gcagcaataa atgacaatac
     7621 atatccaatc gcgaatatga tttgcaaagc aaacccgcga cgtgctccag aaaaaaacgc
     7681 aattagcaat aaaaataaaa tcagcaaact cagcatgcta cgttcctttc tcttctaact
     7741 tttttaaatt tttcttattt tgataaaagg atcttaatta gaagattttt gtttaatttg
     7801 tccttttcgt ttctcgttta aaatctgttg tgcttccaca tgatttttaa tggtatgatc
     7861 tgcttgacca ttttctttca atacttcccg agcttcgttc tcgattgctt caatgcgttt
     7921 cacacgattt tcaagctctg taaatttgat catttttttc tttaattctg ctgtttcttc
     7981 ttccagctcc aagattcttt cttgtttctt caattgatcc gaaagcgcat tgacagccat
     8041 caagattgct gcttgttcat tatccatttg tggtgataat tgtttcaatt ctgctaactg
     8101 ttcattgatc agcttcgtta cgatatccat atgatgcttg gtttcccgtc cgataatagt
     8161 atatgtttgg ttggcaatca ctgctttgta gcgtgttttt tcgtgcgcca tacgaatcat
     8221 ttcctcctat ttgatgaaag ttttatttag tgtaccatga agttggttct ttcaccgttc
     8281 tccacaaata tttttcacat acttaactat tatatgataa actttaaaaa aatgctatgt
     8341 ctcgcaatct cttagaaaac gaaaagtcgt gagttgcggt atagttttat gtataaaaac
     8401 gaggagaatt ttatgagcaa cgtcattatc aaagcaacaa acgaccaact tagtcaaatg
     8461 aaagcatact atcgctctta tttgttaaat aaacagattc cttatactag ctttgctgca
     8521 aaaaaagacg ggacgaccat taccgcttat acatccggaa aagtgatgtt tcaaggtacg
     8581 aatgcagcaa tagaagctga taaatggggg gaggcacagg ataaacaagc caagccagca
     8641 aaaacagcct cttctttgcc aaaaaatatc agtcaattat ccgttttagg tagtgatgag
     8701 gtcggaaatg gcagctattt tggtccgcta acagtatgtg ctgcttatgt gaaacgtgat
     8761 cagcttgcag aattacgtaa acttggtgtt agagattcaa aagagttaaa agatagccaa
     8821 atcatccagc ttgctgacat cttaaaaaaa accatcccct ataaactgct tgtcttatct
     8881 ccagaaaaat acaatgagat ccaaccaaaa tacaatgccg ttcggatgaa agtcgctttg
     8941 cacaatcaag cgatccattt gctgctaaaa aaaatcgagc cagaaaaacc agaagcgatt
     9001 ttgatcgatc agtttacgcc ggaagcaaat tttaaaaaat atgcgcgttt agaaaaaaat
     9061 caattacaag aaaaactgta cttcgttact aaaggagaac aatatcattt agctgttgct
     9121 gctgcttcta tcatcagtcg tgccagcttt ttggaagagt tacacaaagc atcagctgaa
     9181 gctggaatca cccttccatc aggagctgga acgaaatctg accaagtagc ggcaaaacta
     9241 ttggagaaag gcggcatgcc tatgcttgaa aaatatgcca aacttcattt tgccaataca
     9301 gaaaaagctt tgaagctgct aaaaaaataa tggaactgtg acaaaagtca tgtcacagtc
     9361 ccttgttccg aataaacggt gttctcatgc caactcctcg gtcacaagtc acaatagtca
     9421 tcaaacatga gaagctgttt tttgaaattg ctccttgtga cgcggagtta aacggcaggc
     9481 tcacaacctc ttccttaaac ggtgttcaaa agcttgcatc tgcgataata agcccggcca
     9541 aacgaaaaat atgaggagct atttttgttt ggctgggctt atttattact gaaaataagt
     9601 ggtcaatgct tgtgtcaaac ttgcagcaat ctgctgctga taactgtctg ttactaattc
     9661 ctttaaatca ctatcattat tcatataacc catctcgatt aaaatagaag gttgaagatt
     9721 ttctcgaaga acttgatagt tcccagtgga gattcctctt gaatccagag gcagatcagt
     9781 gaaggagtca gcaattgtct gtgctaatgt ttcctcttgt ccatagtaat aatatgtcgt
     9841 gattccagtt gcttcattcg catattccgt agagtctgcg tgtaaactga taaagacatc
     9901 tgcctttttt tcttcactgt agtcgcatat atctccaagc tgtactaatt catctgtagt
     9961 atgggtcaaa taaaccgttg ctccagcatc ctctagcgcc ttttttactt ttatggctgt
    10021 acttaatgtt atatcttttt cttctgtttg ttcatcattg cttaatgccc cagtatcatt
    10081 tccgccatgc ccaggatcta atacaatcac tgcatcagat agctcttccg gttttgtttg
    10141 tatattcttt gtgactttgg ttccatcttc gagggctaaa tatccagtat agccatcatt
    10201 tgtcttgatc tgataagcat tttttgtttc agaaagaaac tccgccagct ctccgccgtt
    10261 tatagttgca agtggggtag ttagcgagtc atctgtatat agtgtacggt cattttttcc
    10321 gattttgatt aatgtctctt gttctgtatc atcaaatgat tcctttgtct ttgtaaccgt
    10381 actgttcgta ctgctgaccg tttgtccaac tatctttttt tgaccaaaaa catctatttg
    10441 aaaacagata ataatgataa tgattgcaaa tacaaaagct ccaattattt ttttcattct
    10501 tgactctcac ctcgtagatc cagactaagg gtcaaaactt aaaatcagct taagtacgca
    10561 aaaaaggggc tgtaaaagat ataaccgtag aaaataacat tactgctaaa atgatttttt
    10621 tcatttttgg catatcctga aatctccttt tgactattcg tggtgattca tctacccaat
    10681 aaatagtttc cttcattcta caaacaatta atttcttcgt tattaaatta aaacacttcc
    10741 tggaacaccg acataataaa taaaaatata ccctgataat catgagagaa taacaaattt
    10801 gtatttatga aaaactacct atataaaaaa aacaagatat aacgatcttc gtcatatctt
    10861 gcttcttcac ctaactattt ttctccctat ataacagctt tacgcttctc ctaatacacc
    10921 attcaaatat aaatcgatca attgcgccac atttagcggt tcttccacag gtcttttcga
    10981 ccgagtgatg taaggtgcta acactaagaa aaaatgcctt gctgcgattt cttttgaaat
    11041 cccatttctt agcacagatt ggtttgctga aaagatttct tcaaaaggtt cacgataatt
    11101 tttcttccat agaaagaaaa gatgatctct tgtttccttt gaaagattgc tttcaagatc
    11161 gtgaagcatc agaaacaaat cgaccagatg tgtctcaatc aaaaactgcg ccatttttgt
    11221 caaggttact tgaaatcccg tttcgcttgt ttgaacgatt tctctcaaag cttttccaac
    11281 atcttttaag tggttttcta aaactgccgt atatagtttt tctttatcac cgaaataatg
    11341 atagagattg ggttgggtga tcccaacttt ttgtgcgatc tctcttgttg aagtgccgag
    11401 atatcccttg gacataaaca agtcagtcgc ttctttcaaa attttttgct gtgtatcttt
    11461 gttgtatcga tggaccattt ttccacctgc tttttggtta ttctttttct ttcaatacac
    11521 catcttccat cataaatatt ctatcgcatt tgtcaatcag ccgtgtatcg tgagtaacca
    11581 tgatcgttgc tttatttttg tcttttgatt cttttgccaa gatatcgact acttctcgtg
    11641 ccttttctga atccaaacta gcagttggtt catctgctag aataatggac ggatcattgt
    11701 acaatgcacg agcgattgcg accctttgcc gttctccacc agatagttct tctggatact
    11761 tgttgatcaa cttatccacg ccaagctgct caaataattc ttttgcttct ttttgacgat
    11821 gttcatgttt gatcttatct actaataata actgcttttt tactgtcaga aaaggaacca
    11881 gattggaagc ttgcaaaata aagccaattt ctttaaaacg tagtttaacg cgttcacttt
    11941 ctttttgatt actgaattct tgatcattga taaatacaga gccactgcta ggcgtctgca
    12001 atccgcctgc caaagtcaaa aaagtacttt tacctgaacc agaaggaccg atgattgcaa
    12061 caaattcccc tttatcgata ctcatggttg tttctttcaa tgctttaact acttggtcgc
    12121 catcttggaa acttttttct acttctttaa attctattgc tttcatgctc ttcctccttc
    12181 ttatccaatt gccttcaacg gatcgatttt tacgattgtc cgaacagaaa atagtgcgcc
    12241 taacacagct actaccagca tcaacaagct aatgacgcca aaaaacagcc aattgctttg
    12301 gaatggaacc gcatcaggta gaacaaggga tgttcccact gttcctaata gaccaagaag
    12361 aataccaaca aatgataaaa tgaatgtttg tgccactaca gaacgcgcaa taaatcctcc
    12421 tgtgatccct tgtgctttca ttacaccaaa gatattaatc ttttgcatcg tcagtacata
    12481 aatgaaaatc ccgatcacaa tagccgcaat aacgatcaaa aatccgatca taaatccaaa
    12541 agtcaatact tgcgcattat atccaggtaa ttcattgatg aatttagaaa ttttgatctg
    12601 ttctaaatct tcaggtaaat tactgatttt acctctgaca actattgcgt taatccgtgc
    12661 attttcactt gtatcttctt tttcgaagcg gatctcttgg taagcattga tggttgtata
    12721 gagaactggt gagacattga atttggcatg atccgtaaag ccagttagtt taaacgtctt
    12781 gtcactacca gataacttaa ctgtatctcc tacggctaga ccatattctt ctttcaaact
    12841 aatatcgcct actgcctcat catcgttatc aaaagctttt ccttcaacaa gattgggcat
    12901 gatgaattga tttttatcga taccgaagaa gctaacattg atttttcctt cttctgtact
    12961 gtctttacta gtcacgactc caggtgtctg ggcaagatac gccactttcc caccttcgac
    13021 ttcttctgct atcttcttag taatcatgga catccctaga tttgaatttg catctttcga
    13081 aagcacaatc gaatctgcct cccacttgtc gactgctgtt cgattatctt gagctaagcc
    13141 ataagctaac ccagtcaaaa aaaataccag gtaggcgatc aaaaacatca cccctgcaac
    13201 taacgcataa cgtaactttg aatgcataat ttcatttaat gctaaaaaca tttaactcct
    13261 cctcctgaaa taatcatttt atacaacaat tttatcactt gataaatttt tggcgaatat
    13321 tatgctcaaa aaattcttcc gctatttcat tatatgaatt ttttattctc gattgattaa
    13381 tatttattat atccccacac aataaaagac cgtgatattt ctcacagtct tttatctact
    13441 ctattgctat ttgctctctt agctagtact ttaaaagcta actcattgag gtagtgaaaa
    13501 cgagaggtgt gttcagacag aaatccaaaa tcctcaactg ctagctgtgg aatcgtagtt
    13561 ttgtgtttca cgatatccgc aagcaggttc aaatctgata ataagaattc ttggcttatc
    13621 agctgtccgc caattatttc taatgtatcc ggatggtaga tcagctttgc atttactgtt
    13681 ttgttttctt catcgtattg tggaaataga tcaaattctc ctgaacacga accggtattt
    13741 tgttccaaga atggcgcttc atcttctgtc agccctacag ttcctaaaaa gtagtctata
    13801 atgactgctg ctgttatctt ttgtctgagg tcgtaagcgg ttggctgacc actcaaagtt
    13861 agcgctactg ctcttgccat atgtacagct cgagtaacta aaggtaaaaa gagatgccgt
    13921 ttatttttgt caaaactgac tgggacgata tctccaatcg catagatgtc cggatcactt
    13981 gtctgcagat attcattgac tatcacagtc tcatcctcat acaaagccac tttttctttt
    14041 aacaatgtcg tattaggacg agtttgggca gaaaaaatga ccatatctgc ctttattgct
    14101 tgagatagaa gttgtacgga tactactttc tcttctgatt ttttccaatc gatcaagaat
    14161 tcatttggat aaaaattgat ttgcttagtt tgcagctgat tttgaaaact gttgatcata
    14221 tccttatcga aatatcttga taaaacattc ggcatataat caactagatg aataatgtat
    14281 cctttatttc tcaaagcatc tgctaattcc aatccaatat aaccagcacc gaagatcact
    14341 acttcttttg cttcttttaa agcggcaagt acttgcttag attgcactag atttttatag
    14401 gtgtagacat tttcagcttc aatttgttta tacaaggtga caggagagga acctgttgct
    14461 acgattaatt tatcataggt tatggtctcg ttttttcctt ctttggatgc gaggatgagc
    14521 tgatgtttgt ctggatcaag ttcagttact ctccattcgg tgatcagttt tgccccactt
    14581 tcttgaagag tccgcatgtt atgggcaact tcagctaaac tacgtatctt tccttttaaa
    14641 taaaggttga ttccatttga aacatagcct agtccatctt tatgatcttc atcgatcagt
    14701 aaaacttctg ctttcggttg aagttttctt agatttaggg cagctgtgat gcctgcatgg
    14761 gaagcaccaa tgatcacgat tttcatgttt tattcatcct taaattcaat ttttctaaat
    14821 aacattcttt taaaaattgg ttcaaatagg ggaaatcata attatattcg ttgctagtga
    14881 atacaaaaac tttagcggac ttctgtgacg ggtactcttt caattggcta gtcaaaatga
    14941 tatcatactg cttttctttc tcgtattcga ctaaatcata tttgatcgta ttcagcaatt
    15001 gttcctggat gatttctttc acgatatccg ccattagcct ttcataagga agatcataag
    15061 caatttgcag acgtaattct aactgcttga tcagcgtccg caaaatagat gcatagcgag
    15121 caaataattc aaagcgggca ttttcctcgt ctacttcagt aaaatcaaca atagcaaatg
    15181 tttgtctgac caatctttca cataaaacca ggaattgttt atcttctact tcagttaacc
    15241 gctgttttaa tccttcttgg ccaaaaatat tcatccatcc tctaaaatag gcaaaatgag
    15301 cgtgattttg catcactgtc aacgcaatct tttgcgaaat ctcttctgga aaaacataaa
    15361 aagagaaata ttcttctatc gattgtttaa acgttcgatt caattgagct actacgtcgt
    15421 ctttgtattt ttcgcttatc aaaaactcct ggacatgcga gttatgtgta tctaatgtaa
    15481 agttactgat catgaaaaga taaaaaacca acgtttcatt ttgattccaa ggaaaggcat
    15541 attgaaagaa gtaacgggtc aaaaatgtat ggaggtccaa taaaaattgt gtcgtttgaa
    15601 acaatccaac aggaagattg ctataatctg gtttggattt tttcgtacag cgcattcgct
    15661 tcttcataat ggaaagccat atttcaaatt tcaatcgtcc ttcttctgta atagtaaaac
    15721 taaactcttt ttgcagcaga tctaaaaaag tgtttcctga taaatcactg tattcttttt
    15781 tgttttcaag ataaggacgt ccaaaccaaa ataattggaa ataaaaataa caaatttgac
    15841 tttctggacc ggtcagccgc ccgcttttta ttttcagatg aaactcattt aaagaatcat
    15901 ttaattcctt cactctgcga gacaaagtag ccgaactgat attaagttcc atgacaagtt
    15961 tctgccgatc aaatgagcca ttttccaaca gataaagaag tatctgatat tttgtagaat
    16021 agcgcaagta gcaatgataa taaacatcga ctgctgctat atttccatgt ctgattaata
    16081 aaatattccc ttcttggata acaatttcca attgtttgat attttccaag ttagcttgta
    16141 aataaaacag atcttctttc aaggtagccc gatcaactga taagcgggta agtacgttat
    16201 taacggaaat ctctcctttt tcctctaata aaagttggaa aagtttgatt tgccgttgtt
    16261 catttttttc aagcaaactg aaaagttcca agtccactcc tccaagcatt tctgatttta
    16321 acagcagaag tttccttctc atccttgaaa aagaggatct gtgacagtta tcacagatcc
    16381 atacaaattc taatcactta gctggttggc ccagctgtca gttcccatgt gaccatgcct
    16441 tgatacgttg cgccaggttg agcattcgta ctgctattta cctctaagag taaaccatcg
    16501 gtatcgggaa agtcaacgag aacctctcct ttagctgttc catcactctc ataaatcaac
    16561 gtgttttgac cttgctgaat caattgtttt gatccagaat gattgaagta taattttgtt
    16621 ccgcttaatt cttgaccttg atcattctta aataaagaag tcaatgtccc tgtcagttgc
    16681 caaggagacg tgcttggaag ccgagtatcc cgcacatcaa aagaaaaatc gccttttgct
    16741 tcataataac ttgtcttcaa cggtttgggg agatcgttac caaatgtaaa atcatctggt
    16801 acattaacta atgttaattc ttgtttgggg acaagaattg ttttagcaga tgtttggaca
    16861 actgggttag gaagcttagc accatctgct aatgtaatat tgctggaaag tacagctgcc
    16921 tttgtcagta tgctgacgga ttgtacagaa gtatctacag tatttaattg atagttgtaa
    16981 gtaagatgaa tggtatctcc tattttgctc aatagatctg ctgggacaga aactccttgt
    17041 tgaatgccgg tatctgcgat agttgtacca tttaatttta aggaaccatt gatataagta
    17101 gtattttcag gaagtgagat cgtgattttt ttgtcggtta tcgcatcccc agattttgtt
    17161 aacgtgaatg tcacattttc ttgaatcgta tctcctgata gagcagacag gggatctcct
    17221 gtctcaggtt gtctgctaag gttttttagc tgactatctg cactcacaga tggaaaatta
    17281 gccactgtta tttgaggaag agtggtgatt ccagcttgtt cagactgcca tttgtacgca
    17341 aaagacggat acatgttagt aagtacatcg tccaccaagc gataatcaaa ataatattgc
    17401 cctgaagcca atgcaggtag tgtagaagta gctgtattta cacccccgtt atttgattgg
    17461 atcgtttgga aaagttgatc tgtccatcct gtcgctaatt tttgggttcc tttcgaataa
    17521 agcctgtaat ggatcgaacc cttttttagt tcagttgctg accaattggc cgtgacttgg
    17581 tacgatgcag aagaaaggag ctttccccac tgactatcgg ctgttaagtt tgttggtaaa
    17641 accgttccgt ctagcttgta ctgctccaat acctttaaag caacaggaga aagcaatccg
    17701 ccttgcgcat ctttcccact gtacacgata ttggctgtct gattcaatcc acttgtgctt
    17761 gctggagtat gagtcagtat tgctccttgt gtaacgtttt ttcctgtaaa atagttgact
    17821 acgcgatcaa agcgactaaa tggcaactga tcaaaacgtc cttcagtcag ccccatcgtc
    17881 aatgtccgac tattggtaga gcctggaagg ttctggttat ttccatccac gtattctgtg
    17941 gtatcagcaa atgtattata ttttcctgtt gaatcacttg ttgagatgta actattccac
    18001 gtatttatct ctgcacttgc agaaaatgca tatgctcctg gattcaagtt tccgccagtt
    18061 aatttaggat aaaatctacc tgcagctgga gctaaaatcg aattgaacat acgatcttgg
    18121 ataatattat tcgaatcata aaaatcaaca gaaaaaacgg cctttttaga catagaaggt
    18181 tgtaatttac ccttggtcaa aattttgttt ggactcgtat catatttatc tccgttttct
    18241 gtcatcttcg cgacattagc tgtaacatca aattgaagcg ggatatcgtc cacgttgaat
    18301 tcgcctcgcg taataatagc aataatatct gccaatagag tatttgtatc ccacgacccc
    18361 atcccaaaac gcaaggtatg gcttcctgct gataataaac taccgctagt aaccttgatg
    18421 ggggcaccgg caatatacaa gtaggcgttg ttccagtcaa ttgcttttaa tacatcactg
    18481 cttgatacat gttccggcaa attgatgatc gccacaaatg caaatccttg tgcagtgata
    18541 ttcaccaaat caagtacatt tgttagtttc acatggcttt cccaatgaaa acggaccgtg
    18601 ccacctttcc aaggatcatt tgaaccatcg ttcaaataag tagcagtcaa tttaggcaat
    18661 tggttagccc cagttagttg gacaagcttc ccgccattga ctgcactacg tccaccattt
    18721 ggaggtacga tattttcata cgtgtatgga tcataagaaa tggcaggagg cgttgctcca
    18781 acagcttctg ccgaaggtga aaagataaat atacttaaaa caaaaagcga agtaaaaaag
    18841 aaggagcgaa aaaatgaaaa tttattttgt agcaacatgt tcatcctcct tttcctttct
    18901 tctccataac aacaaccacg caagaagcca taattctata ccaatgatca aataaagaaa
    18961 acggatcttt tcttcattcg tctccgggaa tacgccatgt tgattagatt ctttataaat
    19021 cggtagctgt gtctgctgtt ttgatgcgcc attgactgaa ccacttggat gcggaactgg
    19081 acttagatca taaaatgtaa tcgtacctga acttttggta tctgcttgga ctggctcttg
    19141 ccagctgaag aacagcaaac ttgcaataat ccctcccaat aagatcgatg ttttcaacga
    19201 aatcacctca tcatgttgtt tctccttttt tctttttctt gtggacccac aaaaacagca
    19261 aaatcaacca taacactaga actgcagcaa ccaatcctgc ataaagccat gggaaagatt
    19321 gttcttgtac tgcaaaaacg gaattttcat ttactttttg tgctttttct tttttgatcg
    19381 taaagttttg tgtaaatcgc cattcattac tgtcgcctga ttttgctatg atttctgcgc
    19441 ggtaatcgcc ggcttttgct ttatcaccag ctaaatcaag attcaagtga aaattcgaat
    19501 ttggtgcaac taaaaattca ttagacgtat tttcaatata aagctgattt tcataaaaga
    19561 tcttgctcgt aaatatcaaa ttgctgataa tcctaggctg aggattttgg aaacgaagct
    19621 gaacagttgg ctttgcgttg acctcgtcca aatctcctga taaaagtttc agatccggtt
    19681 ctatcgtctc ttgactttct tgtaaaacga ctgcgagatt ataagcaata cgatttttga
    19741 tttgtgctcc ttctgtagca ttttctgttt cagcttcttc tttaggtgaa acatgaatac
    19801 ccgctaatac acgtccctta aagctttttg ctgggatttt cacttctata ggtatagttg
    19861 tttctgcatg tgctgataca ttgatttcct ttttcgcaat gagcaaaacg tcacggacat
    19921 caaacggcaa tgtctcatct aacgttgggt ttttttgact ataatcgatc gttccaccat
    19981 cactcgttac cgccggattg acagagacct caaatgttcg ttcttcatct gaagaattgc
    20041 tgataattac ttctagtgtc tgattttgtt ccggtgccac taataaatca aaataaccga
    20101 ttgctttatc tatttggttc tctggaaata ccggtttgat tccaaagtct cctgctgaat
    20161 cctttgcttg gctgattgac ggaaaaatga aaaatagact taaaatagaa aaaaacgaaa
    20221 caaacagtcg tttactttga ttcattgtcc accacatcct ttcaacaaca actgcggtaa
    20281 aaaatccttc acctatagta ttttcaccgc agtttgttga atttatttca tactctatgc
    20341 tactgggcca gccacgaggt tccaagtcaa atcagccgtg aatgttccag ctgctgcttt
    20401 cggcactttc agtgtgatat ttgctggatc ataaacagaa agccatgttc ccagaccttg
    20461 atcttttgct gctgtaaata ttggtgtcgc tgcacttgct ttactcaacc cagttacagt
    20521 ttgactagtt ggtccagtgg attcgttatc tgacgtactt ttaacagtgg atgttccaaa
    20581 atctaattca gcgccttgca aaacttgtga accgtttaca aaatctgaaa ccgaaactgt
    20641 cacgttccac ccttgtgctt ggccatctgc acctacacct cgacgatctg atacttggag
    20701 ataaggtgta tcgttttttg aagcgtctac ttgatacgtt tttgttccgc tttcaatctc
    20761 gtgtgtacca aatggcaatt caggaactac atccaatgtt aaagaacctg tctggccagt
    20821 tgctggattt tccggatctg ctggctctac tggttttgaa ggatcagtag ggttaagagg
    20881 ctcaacggta tttgttggtt cagtaaaggt aatatctcct tttgaagtcg cttgaccctc
    20941 agcaaaagct ggtgttgcca agccgcctag aagaagtgtt gaaagtaata atgtcccaat
    21001 catgttcttt ttcataataa atcactcctt tttcttagtg acctaagtat agtagagaca
    21061 gtatgcaaaa aatcctcatg aattcatccg cttacttgaa attatgaccc tttttgtata
    21121 aaagagaaaa aaacaataaa atcgtttaca tgaatattct tcctttttta ttaagtatga
    21181 aaaaatattt caattttatt ttttcgtttc aaattattct attagtgaca tccttgaact
    21241 ataaaaatag gagtacggca tcatctgccg cactcctatt t
//
LOCUS       DARBDV010000050        20874 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000050 DARBDV010000000
VERSION     DARBDV010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.050"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            591..1280
                     /locus_tag="SCM56_001696"
     CDS             591..1280
                     /locus_tag="SCM56_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1110476.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="SCM56_001697"
     CDS             1267..2475
                     /locus_tag="SCM56_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG1110477.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="SCM56_001698"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="SCM56_001698"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEG1110478.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="SCM56_001699"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="SCM56_001699"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEG1110479.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="SCM56_001700"
     CDS             complement(6578..7273)
                     /locus_tag="SCM56_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEG1110480.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="SCM56_001701"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="SCM56_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEG1110481.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="SCM56_001702"
     CDS             complement(8472..8663)
                     /locus_tag="SCM56_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HEG1110482.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="SCM56_001703"
     CDS             complement(8684..9574)
                     /locus_tag="SCM56_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEG1110483.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="SCM56_001704"
     CDS             complement(9561..10328)
                     /locus_tag="SCM56_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEG1110484.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="SCM56_001705"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="SCM56_001705"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEG1110485.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="SCM56_001706"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="SCM56_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEG1110486.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="SCM56_001707"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="SCM56_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEG1110487.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="SCM56_001708"
     CDS             14899..15282
                     /locus_tag="SCM56_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110488.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="SCM56_001709"
     CDS             complement(15450..16208)
                     /locus_tag="SCM56_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HEG1110489.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="SCM56_001710"
     CDS             complement(16226..17044)
                     /locus_tag="SCM56_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEG1110490.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="SCM56_001711"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="SCM56_001711"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEG1110491.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="SCM56_001712"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="SCM56_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEG1110492.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="SCM56_001713"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="SCM56_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEG1110493.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="SCM56_001714"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="SCM56_001714"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEG1110494.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         5962 a         4253 c         3829 g         6830 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tatattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaa
//
LOCUS       DARBDV010000051        20790 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000051 DARBDV010000000
VERSION     DARBDV010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.051"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            179..2107
                     /locus_tag="SCM56_001715"
     CDS             179..2107
                     /locus_tag="SCM56_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEG1110495.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            2245..2700
                     /locus_tag="SCM56_001716"
     CDS             2245..2700
                     /locus_tag="SCM56_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HEG1110496.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            3170..3439
                     /locus_tag="SCM56_001717"
     CDS             3170..3439
                     /locus_tag="SCM56_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110497.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            3839..4093
                     /locus_tag="SCM56_001718"
     CDS             3839..4093
                     /locus_tag="SCM56_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEG1110498.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(4182..5228)
                     /gene="holA"
                     /locus_tag="SCM56_001719"
     CDS             complement(4182..5228)
                     /gene="holA"
                     /locus_tag="SCM56_001719"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEG1110499.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(5266..7539)
                     /locus_tag="SCM56_001720"
     CDS             complement(5266..7539)
                     /locus_tag="SCM56_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEG1110500.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(7532..8071)
                     /locus_tag="SCM56_001721"
     CDS             complement(7532..8071)
                     /locus_tag="SCM56_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HEG1110501.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(8207..8425)
                     /locus_tag="SCM56_001722"
     CDS             complement(8207..8425)
                     /locus_tag="SCM56_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEG1110502.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(8422..8667)
                     /locus_tag="SCM56_001723"
     CDS             complement(8422..8667)
                     /locus_tag="SCM56_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEG1110503.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(8783..9511)
                     /locus_tag="SCM56_001724"
     CDS             complement(8783..9511)
                     /locus_tag="SCM56_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEG1110504.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            complement(9670..10713)
                     /locus_tag="SCM56_001725"
     CDS             complement(9670..10713)
                     /locus_tag="SCM56_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEG1110505.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            complement(10703..11194)
                     /gene="coaD"
                     /locus_tag="SCM56_001726"
     CDS             complement(10703..11194)
                     /gene="coaD"
                     /locus_tag="SCM56_001726"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEG1110506.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            complement(11191..11751)
                     /gene="rsmD"
                     /locus_tag="SCM56_001727"
     CDS             complement(11191..11751)
                     /gene="rsmD"
                     /locus_tag="SCM56_001727"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEG1110507.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            complement(11870..12208)
                     /locus_tag="SCM56_001728"
     CDS             complement(11870..12208)
                     /locus_tag="SCM56_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEG1110508.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            complement(12192..12629)
                     /locus_tag="SCM56_001729"
     CDS             complement(12192..12629)
                     /locus_tag="SCM56_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEG1110509.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            complement(12619..13743)
                     /locus_tag="SCM56_001730"
     CDS             complement(12619..13743)
                     /locus_tag="SCM56_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110510.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            complement(13764..17192)
                     /locus_tag="SCM56_001731"
     CDS             complement(13764..17192)
                     /locus_tag="SCM56_001731"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HEG1110511.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            complement(17218..18381)
                     /locus_tag="SCM56_001732"
     CDS             complement(17218..18381)
                     /locus_tag="SCM56_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEG1110512.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            complement(18384..18686)
                     /locus_tag="SCM56_001733"
     CDS             complement(18384..18686)
                     /locus_tag="SCM56_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HEG1110513.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            complement(18800..20572)
                     /gene="recQ"
                     /locus_tag="SCM56_001734"
     CDS             complement(18800..20572)
                     /gene="recQ"
                     /locus_tag="SCM56_001734"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEG1110514.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
BASE COUNT         6029 a         4310 c         3577 g         6874 t
ORIGIN      
        1 tcttatttga gaaatgttct ggttttttat tttctttaat aataatcatt ataaataata
       61 aaaacaacat caagcttaat caccaaatga taaccattat cgttctcatt tagctgttat
      121 tattatcaat tacattttta ttatgctatc cttgttttga caaggaggtc taataacgat
      181 gactaatcag aaaaaagctt ttgcagcaac aatcggctgc cttttattta tgatcttcgg
      241 ttttatcttc gaccgattct ctcttaccat caccccactt ttttatatca gtgccattat
      301 catcggtgga tataaacaaa catcggaagg cttgaaagaa ttgtggaatg atcggacact
      361 gaatgtggat ttattgatgg ctttagctgc tattggtgcc tgtttgatcg gtaattactt
      421 tgaaggagca atgttgacat tcatcttttg tttaagcggt gcattagagg aatacacaac
      481 taataaaagt caaaaagaaa ttactgcttt gatgaatctt caaccacaaa aagcccaatt
      541 attaaaagaa aatggacaaa tgcaagaagt agatgtttct caactaaagg tggataactt
      601 ggtatttgtc gctaaaggag cagctgttcc gatagatggc tttttagaaa gcacacaggc
      661 aacgatcgat gagtctgcat taagcggtga atctgtgcct gtagaaaaat atttaggcga
      721 tgagatgtat gcaggaacgc tgaatcaagg aaatcccatc acgattcgag taacaaaaac
      781 gagttccgaa actgtctttg ctaagattat ccaattagta gaagaagcgc agaatacgcc
      841 aactcaaaca gcttcattta tcgaacgaat cgaaaataat tatgtaaaat tgattttgct
      901 tgctgttcct ctaatgatcc ttctccccca ctttttccta ggttggagtt gggatgaaag
      961 cttttatcga ggaatggtat tattagttgt ggcttctcct tgtgcgcttg tcgcatctgc
     1021 tacaccagct tctctagctg ctctttcaaa ttctgcaaaa aatgggattt taatcaaagg
     1081 cggtatccat ttagaaaagc tttctgaatt aaaagcgatt gcctttgata aaacaggaac
     1141 gctcactaaa ggaaaaccag ttgtaactga cagctttttc tttgatgaaa aggatttggc
     1201 acaacaactt cttgttgcta tggaacaaaa aacgacgcat ccacttgctc atgcgatttt
     1261 gcaatatttc gattgcacaa tacctgaaga aatccaatac ttaccaatag aagaaattac
     1321 tggttttgga ttacaaacta cctatttagg tgcgcaatgg aaagttggca agcacgcata
     1381 tgatcctgag acgatgataa tcagcaaaga gattgcggaa atgatcgaac gtttggaaaa
     1441 tcaaggcaaa accgtgattt atctttctaa agatcagcag ttaattgctg ttttaggtct
     1501 gctagatatt ccaaaagcaa atacccaaca agtaatttcc tactttaaat cccaaaacat
     1561 ccatacaagt atgataaccg gcgaccattc tggcacagca aaggccattg ctgaacaagt
     1621 agggattgaa cattactatg caagctgtac tccggaagaa aaaacacaac tagtcaaaaa
     1681 agagaatgaa ctttaccaag taaatgcgat ggttggtgat ggagtcaatg atgcgcctgc
     1741 tttagcagcc gcttctttag gtgtcgctat ggggcaaggg acagatgccg ctatggatat
     1801 cgcagacatc gtcttaatga aaagcgactt aaataaattt gtctacagtc atcggctatc
     1861 tttaaaaatg cggcggatca tcaaacaaaa tattattttt tcgattcttg ttattctctt
     1921 gttgattgct tctaacttcc tgcaattttt aaatctacct cttggagttg taggacacga
     1981 aggaagcaca atattggtta ttctgaatgg cttgcgttta ctaagacctt tacctgatct
     2041 ttctgattct tcaaaaaaat gtaagtcttg tccactatac aaagcgcggc tgtcttctgt
     2101 caaataagca aagtgttttt ttatgatttt gtttatttta tgaaaaaaac agcttatgaa
     2161 taggtcttga aggatgaatt cctacttttg taactttcta aaaacatgtt atgattgttt
     2221 cgaagaaagt gaggaatata tttcatggaa agttggcttt ttttaggaat tattttactt
     2281 gtagcaattt tagctaaaaa tcaaagtctg atgatagcgg cagcttttgt tttgattatg
     2341 aagctcatac cagcctcccg ttcctttttc ccttggcttc aaggcaaagg aatcaatatt
     2401 ggggtgacga tcatcacagc ggctattcta atccctatcg caacaggaga aatcggtcta
     2461 cgtgatctat tagaagcctt taaatcccct attggctggg tagctgtttt ttgcggtggg
     2521 ctagtggctg tcttatcttc caagggtgta ggattgattg cttctgatcc acaaatcact
     2581 gttgcacttg ttttcggcac gattttaggt gttgtcttct taagaggaat cgccgccgga
     2641 ccggtcattg cttctggtat cacttactgt atcttacaag tatttgcttt aggaaaatag
     2701 aacaaatttt gtctatttta ggagattctt aacaccccca atttactgat ccgtgatata
     2761 ctgacaacga ggtgatacaa tgttcgaaaa attaaagtat ttcataggaa caatcggctg
     2821 gtttcttcta gcggtacttt tgattctggg cttagcttat ctattatttc ctgatttgat
     2881 acaaaatgtg tttgatactg ctttttgatt gacctattaa aacgatatag gggatgctcc
     2941 tgcgatgcag gggctttttt taatcaatgt tttgtaataa tttaggccgt gccaatgaga
     3001 cttcctatag aattcgatgc tcttttttct ttttggctct tctgctcttt tttgttatac
     3061 tagattacag tggacaaaag aaagaatgat cgcatccact tcaacgaata tcaaaatgga
     3121 agaattagtg cctgttttca ggttactttg aaaagaaagg aagacgcttg tggaaacttt
     3181 tgaaggtctt ttaattgata cgtacgtttt acaagaacag ccaatgatgc tgactgggat
     3241 caacttgcag cttgaggacc gtgtgatcaa tggcctgctt aaagatcgct atttggcaaa
     3301 atatatctta cgtttgcccc taaataaata tgtgctggaa gtaagtggca aatggaaccg
     3361 tcggaaccag ttggtcataa aaaaaatgtt gataaaaaat atcgatgaat acattaaaat
     3421 aatcggtaca ccagaatagc ctttactgat caaaggatac tggttataaa ataactgcag
     3481 tttcttaata gaaaaacaaa gaacaacagg gaatcgtatc ccttttcaaa aaaaactttg
     3541 caaacggttc tctttgttat ataataactt agtaccaaac attcccattc aaactttgtt
     3601 taaatagata aataaaaatc tagtttggtt tgacgctatt gcctcccaca atagcgtctt
     3661 tttgattttt ttcagcttgt actatccgtt tttactgaaa aaaaatccta ataaaagact
     3721 gttaaggaaa aaaacttgtc atgttcatat tgcaaagcac gctgccttta ggtataataa
     3781 tgaatgttga attatgcggt aacacgcgta agaacttaag ttatcggagg tgaaactaat
     3841 gccaaacatt gaatctgcaa tcaaacgtgt tcgtacaagc gaaaacgcta atgtgaaaaa
     3901 ttcatctcaa acaagcgcta tgcgtactgc aatcaaaaaa tttgaagatg ctgtagcttc
     3961 aggtgctgac aacgtagatg cgttatataa agaagctgtt aaagctattg atatggctga
     4021 atcaaaaggt ctaatccaca aaaacaaagc taaccgcgac aaatctcgtt taagcaaaaa
     4081 aatcgctaaa taaaagaatc ggcttcggcc gattttttta tttgtcaaaa aaaggacaaa
     4141 aacaaacgac cttcccgcat gctttgccct cgtctttctt cttatcgatt tcttaacaac
     4201 tgccctgtgc gcaaaacgaa tagttgaaaa cttaattctt tttcgatttg tcccgttttg
     4261 atttgataat ccatctcaac taattgatca aacaactgta ccagcaaatg ttcatcgaat
     4321 ttttttactt cctgcatggc aagttttaca cgatatggat ggattttcaa tgtttcagca
     4381 atatttgcct gctgataacc aatctttaca agaaattttg tctgtaaaaa taaacgtaat
     4441 tgagaaagca aaatcgcatt aattttgatt gtttcttccc cttgcataac taaatcttcg
     4501 tataaacgca aagcttgttc cgtcttccct tttaaaatat attgggtcat gtcaaaaata
     4561 ttatgttcta acgttttagg aactaattct tctacttctc ttaccgtgat ttgctgttga
     4621 ttttgtccat aaaggaccaa tttctgtaac tctctcatca ttttggataa atctaagtcg
     4681 gtcaacctca agaataaatc aattgcactt cgttccattt tgattgagga attctctaag
     4741 gtattcatca gatattgacg tacttctttt tcattcaact gttggacatt gatgataatt
     4801 gctgtttttt tcaattgttt cgttactttt ttccgttcat ccaacttttc atatggcgca
     4861 aagaatacta atacagtcga ttctaatggt tctttcaaat aagccgtcaa taaatcagta
     4921 ttttgttcga ttccattgtt tactttttca cctgttaaaa aataaggatt ctctacgaat
     4981 actaaccgtt gatctccgaa aaacggcaac gtctctgctt cagctactac ctcatctaaa
     5041 cttgcatttt ccatgtcaaa acttaaaaaa tttaagtcat cttcgccctc taattggatt
     5101 tttttgacaa tttctgtccg aacttgatct tggagaaact tttctgttcc taaaatcaag
     5161 taacaagatg caagtttttc tttacgtatt ttttgtaact ctgcttgtat actcaaatta
     5221 ctcaccactt tcaaaaatta tcctaccatg agcagatgag aattactagt ctatcattgt
     5281 ttccactagc ccatgaggat gaaaaactgg atgccagcga taggtgatca ttccttgttg
     5341 atccgttcgc catgttttta tctgattttc ttctagtgtc tccaatactt cgctgtgcgg
     5401 gtgaccataa cgattattta cgccgcagga tataatccca tgttttgctt caatatggga
     5461 aagaaaatct gtgttacttg acgtacggct accgtgatga cctaatttga ctacatcggc
     5521 tttcagatta ggataaatca aaagcaattg tttttccatt gcttgattca aatctcctaa
     5581 aaataaaaag cgcctattgt tatattccat ccatagacca atcgaatctt catttcttcc
     5641 ctctcccttt tcaaaaggag tcaatacata aagatcataa taaccgctga tcctgtcccc
     5701 atgtttgatt tcaatgatct cagtaccatt tttttgagct tcagataaat gcctttgtaa
     5761 tggctcattt cgtccagcgc cccaaccgat ataaattttt ttgatagtaa aatgctgaga
     5821 taacgtgggt aattctccca catgatccgt atcgtcgtgt gtcaagatga gtttatcgat
     5881 tacacttatc ccctgacctt ttagatatgg aacgatattt ctttcagcag gaggacgtat
     5941 cataccttgc gcccactttt ctctttcaaa gtgtaactga cctccagtat cgatcaaaat
     6001 cgtttctttt cgaaaaggac tttgcaaaaa gatactgtct ccttgaccga catcgataaa
     6061 agcgatcgtt gaagaaaagc gtaaaaatgg caaagtcaaa aaaataaaag aaaaaagacc
     6121 aacataaaac aagtgtttca caggttgttc tttcaaccta tccatacaaa tcagtaatcc
     6181 gaaaatatac atcccagata gcacaagggg caaacgtcct ataatgagag acgaccctga
     6241 agaaaaatcg attattcgct caaataagat taatccctct tctatcagca aaagtaaagt
     6301 ttccggcagc cagcgattga agagtgtgag aaataacaca ccaggaagaa aaagaaactt
     6361 gaataccggt gcaatcagta atgtaaaaag actacctaaa ataggccact cataaaaatg
     6421 ccatacaatc aacggcatcg tgatcatagt aaacaggacg tgagaggccg tttttttcaa
     6481 ccaatctcca tcaaaagaaa tcaggataaa caaaaaactc atgaaaaaac taagctgtcc
     6541 acccgcatga aaaagaaatc gaggatcaaa tagtaaaaga agccaaaaga caactgcaaa
     6601 taagtccatg gtggaataat gcttattcag caatttgact agcaatcgca ataaaaacag
     6661 caaactagca cgcaaaacag aaatagaact tcctgcaata gaaacataac agatagttag
     6721 tagactcaac ggaataatac tgacgtgtag tggccatccg atacgtcgaa ataaataata
     6781 cagccaccct aaataaaatt ggatgtgtag acccgataga ctgaacagat gcagcaaacc
     6841 actctcccta aaaatttctt ctggttctct gaatcctgca tctttatagc cccataataa
     6901 agaattaata tatacacttg ttttttgcgg aaattcagat tggacaaagg agataaaaga
     6961 tcctcttttt ctctgaagcc agtttcgcca gtgattttgc atggtgatct gttcaatctt
     7021 ttgaatttgg aaccgtccag aaattctatg gttcctgaag taagcttctt ggtcaaaatt
     7081 ataaagattc cgcttttttg ctggagagat gaactcgcct tcagcagtca attctattcc
     7141 ctttcctcgg aagttaagcc atgttttcaa ttctgcttct gtgtttactt gataagaaag
     7201 ctggagcttt tgtttattct ctgataacct tccttccatt gtcaaaaaat taccatttgc
     7261 acgaatcgta tctgtaaata cagtaatttg cgcagatgtt gttttttcct ctaaaaatga
     7321 atactcttgt agcctataag aaatggatac cgttatgcta cacaaaatac acaagtacgc
     7381 gataaaacgt ccttctttta gcgaaaacca taaaaaaatt ccggcaagaa gcaaacgata
     7441 accccaattg tcagtgtaat acaacaaaca gataatcatc gacgtaattg ccggaaaaat
     7501 caaccgattt cgaacatgcc tttctaaaac gctagacatg ttttcctgtt aattcatttt
     7561 gaagttttga aaaatgctgt gagtctaaag caacttggtg tacagataca cctaattgtt
     7621 cgatcaacgc ttgggcataa ggatcatttc gatagtcatg gagataattg atccctttca
     7681 caccagcttg caacaaagcc tttgtacagg ccaaacatgg aaaatgtgtg acatagattt
     7741 ctgaaccttc cgtcgggata ccaagttttg cacactgtag gagtgcattc atctctgcat
     7801 ggatcgttcg caagcagtgt ccatccacga cgtaacaccc ttcatctatg cagtggacat
     7861 ctccactgac tgctccatta tagccgccag caatgatcct tttttctcga acgagtgtcg
     7921 ctcctacttc taaacgtgta cacgtactac gtaaagataa cagtactgcc tgagccataa
     7981 agtactggtc ccaaggaatg cgttcattat gtgacttatc agtaactttt tctttattcg
     8041 tatcgcatgc ttttgaagat ttttctaaca tgttttatat tcctccgttt tttcaagttg
     8101 attaaaaagt actcattgct taaacaaaat attctttaaa tataacgaat catcctgtct
     8161 gacaaaagat cctacccttc actgccgaaa tgaagtccgt catccattag cccattataa
     8221 aaattcttcg aatccatttg ttcaagaaat tccgtataaa aatcgatact tcacaatcat
     8281 ggcgttttgc tacttctgtg agaacagaga tatattcttt gatcaacttg ttattccgat
     8341 atttttgttt tgtctcatcg actgctggtg gcgtgataaa gaaagcatga tcagaactat
     8401 agtctttttt cagctgaact atcatccact gcagattttt tggtacgttt ctttagaaag
     8461 ttgtttatgc tctgctagat cattcgtccc aatgagaata aatactttat ctgctgtata
     8521 gctaagtaca tctttggcaa agcgttctcg tagagcttga ctgttattgc cgctgacagc
     8581 cgtattcata atatgaatcc cagatatttt attttggaga tagacattca gcattggttt
     8641 ttttaatgtt tctttgcgaa caatcaagct atttcctatc caaaaatact acctcctcgt
     8701 cctgccaagc tcttagaatt ggattattct tagtatccat tagcgtccat ttattgctcg
     8761 tgccagtcga ttttgaatca ttctatttaa ttgtaacgaa gttttttagt ttttcaactg
     8821 tcttttcacc gataccagaa atctcctgca ggtcttccac tttctggaag gatccgtttt
     8881 cttctctaaa tcgaatgatt tcttgcgctt tgactgatcc gatcccagga atctgctgaa
     8941 gttctgatgg agtggctgta ttgatattta ttttgggagg ctcggaatct gctgtaggct
     9001 gttttccttc cgaggatgcc aactgatcaa gatgttctgg cagctcttca cctacagcag
     9061 ggatatatag gacttgctga tcttctatct tcgcagaaaa gttgattcga tcgctatccg
     9121 cttcttctaa taatccacct gcacgcttta gtacatcata gacccgttct tctgaagaaa
     9181 aggaatagat accaggctcc ttcacacttc ctttgatatc ggtgtacatg acctttggac
     9241 gagcatcctc ttctacatcg tttccttcag ctgatggatc ttctccactg gaactaaacg
     9301 aagaaagtat ttcttttgca gcagtctctt ctgtttctct ataaaatagc aaatttgata
     9361 ataaaaaacc acttgcgata caaaagataa gaactatcat agacatccaa atgatcaatt
     9421 tgggtgaagc tagtattttc ttgctgattt tttctatcat tgttcttttt tcaaagaatg
     9481 accagtaaaa atcattcatt actcctttca tttaaaattg aagaaacagt gagctctggc
     9541 aaaatcgtcc cttcaacatc taattacgtc aattttttct tttgttttta tttttatcgt
     9601 gaagattttt gacgaaaaag aggggttgtg acagaactcg tgtcacaacc cctctttaat
     9661 tatttagttt catttcattt tctctagata gtcaagcgct tcttgtactg tttttactgg
     9721 tacgattttc attgaggaat tcaatttttt agcggcagct tttgcttctt cgtagttggt
     9781 ttttatttca ggatattctt tcttaacatc gtctgaaatc gtatcatctg gtgcaaagaa
     9841 aatctctacg ccagctttat ccgcacttgc cactttttta tcgattcctc cgattcggcc
     9901 aacttctcct tcactgttga tcgttccagt tcctgcaatt tgcttaccat gtcgcaagtt
     9961 tttaccagtg acttgttcat agatttctaa agtgaacatc aatcccgctg atggcccacc
    10021 gatagagcca gaattaatac gaacatctgt atctgattcg atctcggtat ggtcagtaag
    10081 cgtgatacct attcctgcct ttttatctgt tggtaactcg atcagatcac cagaagctgt
    10141 tttttcttca ccattatgca aataactgat ttcgattgtt tgtccaactt tttggctttt
    10201 tacataatcc ataaatgctt ctgtactttg aaagcttttc ccgtctactt ttgttactgt
    10261 atctccaact gaaagcttgc caaagaaatt cgatgttttc tctacactca tgacatatac
    10321 gcctttgaat tccattttat aaggcttgtc tgcaagtttt aacgcttgtt cgattgctgc
    10381 attttgggaa gaatccatat aatactgttg catttgatta tattcttcat ttgtcgcccc
    10441 accagtcaat tcttcttcac tgatcaattc ttcaaaaggg gtcattttag cttttaaagc
    10501 taagaaacct gtagcttgcc tgataccaac agaggtaaga gagaaagacc cagaaccttt
    10561 atcttcctta tcattgaccg taattagttc tttcagatcg atcgtggctc ccggtgattc
    10621 tacataatac ggcaagggaa ctactacaaa tccaatgatc aacattccaa atatgataat
    10681 cagtactgtt ttcaaccatt gtttagcttt catttgattc acgctttcca gctaaagctt
    10741 cgttgatcac tggcggtaag tagctgctca catctccgcc aaagtgcagt acttctttta
    10801 aaatactgga actcacgtga ctatattctg gttttgctaa taagaagaca gtttccaatt
    10861 catcatatag atgatgattc atctgagcaa tctctcgttc atattcaaag tctttgatac
    10921 ttcgtatgcc tctgatcaac gcatctgcac caatctcttt tgctgtttct accgtcaatt
    10981 gattttcttg atgcatgact tttacattag gaagatgaga aacagctttt gtgatcaatg
    11041 tcattctttc ttctggggga aacaaacttt tctttgaagt attgataaaa acaccaatta
    11101 ctacctcatc gaataattta gccgcacgtt ccaccgtatc taagtgacct tttgtaaaag
    11161 gatcaaaact tcctgggaat aatgcacgtt tcattgattt ccctcttttc tatagatagt
    11221 tacggccgtt atgccataaa tctgttttct tatttgatgg aatccagcca cagtttctgg
    11281 caattcgact gttttatccg tttcgcatac gataactgcg gttttagaaa aaagtcccag
    11341 ttcttccatt ttatggattt gatcgattat tttttgtttt gcatatggcg gatcaagaaa
    11401 caagtagtcg aattgttgtt tctccgctgc tagccgttcg attgctttgt ccgcatcaag
    11461 ttttattgtg gtaaatttct ctggttcttt cgtaatttcg atgttttctt tgatgatttt
    11521 tagcgcttgg taatttttgt ctacgcatac cgcatgactg cagcctctag atactgcttc
    11581 gatagctaaa ccaccgcttc ctgcaaacag atccaatact gtctctccat caaaatatgg
    11641 tccaatcata ttgaatatgg attcttttac tttatctgta gttggtctgg tgttcgcccc
    11701 tgctaaactt tttaaacggc gcccgccata atttcctgat attatccgca tctactcact
    11761 ccttagaaat ggtactggta ctagtatacc aaaaaaatac ttggacaatg atcaaaaatc
    11821 gatcgttatc caagttattt tttatttaaa aactgctcat aagagatttt taaagatgtt
    11881 cagctaatcg gatttctgta tttagctctt caatttcgaa accttcgtct tttaatactg
    11941 ttttgtttcc tattttttcg gcaaaattca tttcgatatc cggtcggtaa gaacgttcta
    12001 cgtttcgaac aaaatgtaat ttacggattc gttcttctgt ttcttccaat gtctcttcat
    12061 ttaagtaaag cacgacatat ttcattcttc ttgagacata atgcaacaag ccgaactttt
    12121 tcagattctt taattgtttt aagctgtaga cccatacgat cagcccgcgt cgttttgtta
    12181 tttcaaattc actattgtct aattgcatga caattacctc cgcatccaaa gtgttttttc
    12241 gtttcaaaaa acgggctacc tgcatctact ttgatttcat tagaaacttc ttcggcaagt
    12301 acttggacaa tttgatccaa cagactttgc agctgtgttt cactatatcg gaaagctgca
    12361 acatgctcat ccatatcaag ctgtcttttt gcttttcgaa cagctcgttt tttttcacga
    12421 taatcaggtg catactcacc atatgccgct acattttcaa atgcttcttt cttagacaag
    12481 aacgcttctt tcaactggca aacttttgca gattcttcca ttgactgttt atgttcaaga
    12541 taagaagcca tcgttttact cttttttatc atttcagcga cttggaggca ttgatcctcc
    12601 agttgaaaca gctcttcgtt aacgatcact gctatcacta ctttctccgt tttcttcttg
    12661 caataagact aacatatttt ctttagaaaa agcaatgcct acagaatccg ttcccgggtc
    12721 catgaaacgg tcatggttgt aaggatcgct gtaccaatat aaaaacgaaa aagtagcgtc
    12781 aatcgccgga tgaataaata gcccactagt attttctaat ttttgtttac tgattaactt
    12841 ctccaattca tcattcgtca gtgagaattt cactgtcgtt tgagcactgc ctagcgattt
    12901 ttgccaatca gccatacgtt cctctgacaa tatttcctca ggccttgtca aaaaatcatg
    12961 tagtagcagc tgactgtggt tttgaaaggc aagtgtttga ttgtatatgg gaagttcgtc
    13021 ttctcttcta agcatattta ataaagtcgt actttttcgt tctttaacgc gattgattga
    13081 ttcccaatca gccgatttat ctgcttgata atgtggaagg ccttcaccaa acacttgata
    13141 tggcaatagt ttcaaaagac tatctttatc taaatacaca actgaaaata ggccgtttgt
    13201 tgtttgatca aaaaataaaa tagcaaaagt gtcgttatca aaagcaatta gcggacgata
    13261 attcatatcc tcttccatta gttcgattcc aacttctgta tcatggtact caaaagtgaa
    13321 attagtatag atagtagtga ttttcgtaag atcttgcatc gtcatcccta aataaaacgg
    13381 cgcgatatca gcaggccctg tttgcagtac tttaacagtt gtgactctgc tgtcttggat
    13441 gttcgcttca aatgtgagat gactagcttt attttcataa tatcttgttt caaaaccaaa
    13501 tccgctagtt aatcgatcat aggcttggcc aaaatccttt tccagctgat cgatcggttt
    13561 tccgatataa gtagaaaaac cagttgcttt aagctcctgg tatttccacg ttgtcatttg
    13621 gtttgatatt tgttgatcag agttttcctt cacttcaagt tttggcggaa acaagactgg
    13681 ttccatgtag aatgccacaa taactaatag cagaatcagc caaaacaaac ccagtctttt
    13741 catgtttccc cacctttcgc ttattatttc tcagtcactt caatcaaaag gtcaccagac
    13801 gaaatagtgt ctcctgaaga cacatataca tgtgcgactt ctccgtcaaa acgtgcttcg
    13861 atggccgttt ccattttcat cgcttcagta atcatcaacg tatcaccttt tttaacgtta
    13921 tcgccttttt tcactaatac atccaaaaca gaaccagaca tcgttgctcc aatttgttct
    13981 cggtttgtcg gttcagcttt caatcgggtt tggaccgtac tgatgatcga attatctttg
    14041 atagtgattt cacgtctttg tccattcaaa ttgaagaaca gtacgcgatt cccttcgata
    14101 tcgggttctc cgatttcatc taaacgaatg ataagcgttt ttccgcgttc gatttgtaca
    14161 ttgattgttt cacccaaacg tataccttgg aaaaatgttg gtgtgtccag taatgtaaca
    14221 tcgccaaatt gattatatgc tttttgatag tccaaaaaga cttgcggata catcaaatag
    14281 cttaatactt cgtcttgttt tggttcatag ccaatttttt ctgccaattc tttttttact
    14341 tcgttgaagt cgactgattc agccaaaaga cctggacgtt cattcaatgc aggtctgcct
    14401 ttaagaataa tcttttgcaa ttttttagga aatccgccaa cgggttgccc tagttctcct
    14461 tggaagaaag tcacaacaga ttcagggaaa cttagtgttt cgcctttttc ataaatatct
    14521 tcttctgtaa gatcattttg gaccataaat agtgccatat ctccaactac tttagatgat
    14581 ggagttactt taacgatatc gccaaacatc aagttgacag tatgatacat ttgtttgatt
    14641 tcatcccagc gatgacctaa tccgacagct tttgcctgtt gctgtagatt cgaatactgt
    14701 cctcctggca tttcatgcat atatacttct gtttcaggag cattcaagcc gttttcaaac
    14761 ggtttgtaat acatgcgcac atcttcccaa taatggttta atttctgtgc attttcaatc
    14821 gtgatttctg gcagccgtgg accattgacc agtgcataat acaagctgct catgctaggc
    14881 tgactagtat tcccactcat tgcactcatt gccacgtcaa cgatgtctac tcctgctttt
    14941 gaagcagcag aataagtgat gattccattc ccgctggtat catgagtatg cagatggatt
    15001 ggcagatctg tcgtttcttt caattcacta atcaaacgat aagctgcctg cggtttaagc
    15061 agcccagcca tatctttgat tgcaatgata tgcgcaccca gttgttctag ttcctttgcc
    15121 atgtctttat aatactgaac attgtatttt gcccgacttg gatcgttgat atcgccagta
    15181 tagcaaatag ctgcctctgc gattttacca gtatcccgta cagcttggat acttttttcc
    15241 atttgtggag tccagttcaa actatcaaag attcgaaata catcgattcc ttgcgctgct
    15301 gcttctttga caaattccac caatacattg tcaggataat tagaataccc aacggcattt
    15361 gaacctctga aaagcatttg cagcaacgta ttaggcatta aagaacgaat ttttctcaac
    15421 cgttgccaag gatcttcatt caaaaagcgg taagccacat caaatgttgc tcccccccac
    15481 atctcacttg aaaagagttc aggaagacct tctccagtca aacgtgcaat ctgcttgaaa
    15541 tcctttgtcc gaacacgagt tgctagcaga ctttgatggg cgtctctaaa tgtcgtatcc
    15601 gtcaacaaca aattttcctg tcctttgatc cattcgacca aagcatcggc acccttcgca
    15661 tctaatacat tcttagccgt tacgtaatca gttcttgtga ttagatcttt aggcatacgt
    15721 ggttcttcat aaaatggttt ttcgccactt tcgatacctg gaaaaccatt cacagtaatt
    15781 tcaccgatat atttcatcgt tttgtttccg cggtctcgta gtcgaggaaa ttcaaataat
    15841 gttgctgtac tatcaatgaa ggttgttttt gcatttcctg attggaattc aggatgtgtg
    15901 atgacattga gcataaatgg aatgtttgtt ttcactccac ggattctaaa ttctttcaag
    15961 caacgttcca ttttctgaat ggctgtttca aaggtagcac catgcgtaca tacttttact
    16021 agcaatgaat caaaatacgg tgtcactaca tagcccgcgt aagcatttcc gacatccaga
    16081 cgtacgccaa aaccaccagg tgaacgatac gtatcgattt tccctgtgtc tggtaaaaag
    16141 ttattcaacg ggtcttctgt agtgattcgg cattgaatgg ctgaaccatt taatttaatt
    16201 ccttcttgct gaggcaatcc gatttcttta tgcagatcca aaccttgagc aatcaacagt
    16261 tgcgttgtta cgatatcaat atctgtaatc atttctgtga tcgtatgttc cacttggacg
    16321 cgagggttga cttcgataaa gtaaaaatca tctccttcaa caagaaattc tactgtacct
    16381 gcgttgacat acccgacata tttcatcaat tggacagctg cctggcagat tttttgacgt
    16441 tgctgttcat tcatagagac acaaggcgct acttctacta ctttttggtg acggcgctga
    16501 acagaacaat ctctttcaaa aagatggatg acgtttccat gcgtatcccc gagaatctgt
    16561 acttcgatat gcttaggatt agcgatgtat ttttcaacat acacttcatc actaccaaag
    16621 gctgcttttg cttcgctttt cgcacgttcg tagccctctc ttgcactctt ttcatcgtga
    16681 gctacacgca tgccgcgtcc gccgccgcct agagctgcct tgatcataat cggataaccg
    16741 tattgtttcg caaactccaa ggcatcgtct atagaagcga ttggtccatc tgtgcctgga
    16801 atcgaagcaa tccccgcttc gatagctgca gcttttgctt tgattttatc tccaaaaata
    16861 tctaaatgat gcagtttggg tccaacaaaa atgatgcctt cttcttcgca ccgttgcgca
    16921 aaattcaagt tttccgacaa aagaccgtaa cccggatgaa tagcgtcagc tccgcattct
    16981 ttcgcgatag aaatgatccc ttcaatatct agatacgcat caatcggttt ttttccttgt
    17041 cctactaaat atgcttcatc cgctttgaaa cggtgtacgg aatattcatc ttcttctgca
    17101 tagattccaa cagttttaat tcctaattct gtacaagcac gaaatacacg tacagcgatt
    17161 tcacccctgt tagcaactaa aacttttttc atcgaaccac tcctacatct ttttttatta
    17221 taaacctaag ctcttgcgtt tttcatccgc actgatgttg aggacaaagc cgacacaaat
    17281 cgaaagcatc agcaaactgg agcctccttg acttaaaaat gggaacgtga tccctgtcaa
    17341 aggaataacg ccagtaattc ctccaagatt gacaaatact tggatcaaaa acattgcgcc
    17401 gatcccaatg cacaaaagcg aattgaacgg atcttttgaa cggataccta caaggacaat
    17461 gcgcacaatc ataaagaata gcaacgctaa tatcaaaatc cccataatca ctcccagttc
    17521 ttccactacg attgcataaa taaaatcggt ttgtgcttct tgtaaaaatc cttttttctg
    17581 gatactgtta cctaatcccc tgccaaatag tcctccgttg aacatggcat agtagccgtt
    17641 gactagttga tgccctttat ttaattcgtc tgagaaagga ttttggtaaa tagcaaatcg
    17701 attatagata tactgtaaac gtccgggtaa gatctttccg tttgtgatat tgatcagcca
    17761 gatcgtaaaa gtacttagac aaaagcctcc tactcctaca atcaaagtat acacataatt
    17821 gactccgctg gctaaaagca gaaccaaaat aatcaaaaag ataactgccg cattaccaaa
    17881 atcgggaaga atagcgacta atgcagtaag tccgataacc attgcaagtg gacgtttcac
    17941 agtcccaaga aaatcttttt gtacactgtt ttgtctttta gataaagtca gagacaggta
    18001 ccagatagaa atgattttta ggtattcggc aggttggatc gagaatcctg caatttgcag
    18061 ccaacctttc gccccattga tctccttacc aaaaaacaca acaatcagca gcaaaatcgt
    18121 caaaactgcg atggctgcca tgatcaaacg ctggtttttc aaaacatctg ttttcatttt
    18181 ataaagtagt gcgatagcaa tcaagctaag cacccaaaaa atactctggt taatgactga
    18241 tgcggacgga ttttgtccgt tttctaataa tagatacgac gtagaactat ataccatgat
    18301 caatcccaat acacataaaa ttaaataggg aatcaagatg ctatagtcta atagatgcct
    18361 ttttttcact ttcttaggca agtctattcc tccttgccaa cttctttttt atcataaatt
    18421 tcttggtaaa cctcactata taactgattc aattctcgtt ctagctcact taacatcagc
    18481 tggccctcgt tctttccgat tactcctata cggacagcaa aaccgacttg ccgggaaaag
    18541 ccgtacatct gtgtatctac cacttcttcg aaggctttgc attgtgagat acagagactg
    18601 ttgttttgat ttcggattaa tcgtttgatc cgttctgctt catcatttaa tacttggatc
    18661 gcaagttttt tcgacaaaga atccatctgt gcttacctcc cattctaatt ccttaattat
    18721 aacatatttg attttcataa ggagcaaaaa aaacagattt aaccagttgt ttttattcat
    18781 ttttacgtat tcgttctttc tacttatcca gaacgttaga gtccgcattt tttatccgag
    18841 ataaaaaggc ttcgccatat ttatcaagct tgctttggcc aactcctttt atctgaagca
    18901 tctccaatct gcttgatgga cgtattcggc tcatttcttt caatgtggaa tctgaaaata
    18961 cgacatacgg gggaacgcct gcttctgatg ctagatccgt tcgcagttcc cgcagcactt
    19021 caaatagtgt atcggtttct tcatccgata gctgctggac tttcttgggt tcctttcgat
    19081 aaacagattc ttgtcctttc aatacttgta tccctctttc tgttactttc aaaacaggat
    19141 actcaccatt gatggtcaga agatagccat tcgatatcaa atattcaatt aactgcatcg
    19201 tttctttttg cgattggttt ttcatcagtc cataggtaga taaatgtcca aaacctaaag
    19261 cttgcagctt ttgttctttc gatcctgcta agactttcat cagcatttgt ttaccatagt
    19321 tctcacccat acgttttaag caagaaagaa ccatctgtgc ttctgtcgtt acttctacta
    19381 tttctctgtc atctaaacag ttaccacaac ggttgcacgg actgcttgtc tcgccaaaat
    19441 agtttaggat gtattgctga agacaggact caatatacac gtattctgtc atcgctttta
    19501 atttttcata ttctttttgc ttttgccctt cttctctttg ggactgctgg ataaaaaact
    19561 tttggacttg gatatcttga ggagagaaaa gcaggatggc ttcactggaa agaccatctc
    19621 ttcccgcccg accagcttcc tgatagtatg cttctagaga gccaggaatt tgataatgga
    19681 taacaaaacg gacattgctt ttatttatac ccataccaaa tgcatttgtc gctacaatca
    19741 gctgcaagcg atcgtataaa aaggcttctt gcatttctgt tcgctcattc tcatttagac
    19801 caccatgata tctacccacg gaaaaatcga attttttaag caaatgatag agacggtcta
    19861 cttcttttct cgttgctgca taaatgatac cagattgatt tttgttgatc tttaaatatt
    19921 cgattagata ttgttctttt ttttgatctt taattacttg aaatcgtaga ttttctcgtt
    19981 caaaaccagt ctgaatatgg ttgttttctg gaatttttaa aagtcttttt atgtcagcag
    20041 ctacttgcac agtcgctgta gctgtcaaag caacaattac aggacgattt ggcagttgat
    20101 ctagctggtt cgccatctgt aaataacttg ggcggaaatc atgcccccac tgagagatac
    20161 aatgtgcctc gtcaatagcg atcatacgga cgttccatcg cttcattgct tggataaaat
    20221 ccggcatgat aaaacgttca ggagcgatat aaagtaaacg gtactgctgt ctatcgattt
    20281 cttggaagcg tctagctgtt tcgtaaccat ctaacgagct attgataaaa gtggcgggta
    20341 tacctaattg attcgccgcg tccacctgat ccttcatcaa agagattaaa ggggacacaa
    20401 ccacagtaag cccatctaat agtaaagctg gaagctggta acagattgat ttcccacttc
    20461 cggtaggcat gattcctaat acattttcct gatcaatgac tttctggata atttcttttt
    20521 gaccaggtct aaactcatca taaccaaagt attgttttaa taagtcgttc atcttcccgc
    20581 tcctttctct tgccatttta cttcgatttt cttcgaagga caagttgatc tcctttcgtg
    20641 aaacattatc ttattatgaa acaaaaactc tctagaaata agttttttaa aagtctttta
    20701 aacgtctttc ggcgtatttt ctgaatttat gtccattgac aaagatgact tctccacaga
    20761 aaagagttgg acaaaaaaag aaaccagcta
//
LOCUS       DARBDV010000052        20678 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000052 DARBDV010000000
VERSION     DARBDV010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.052"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            99..293
                     /locus_tag="SCM56_001735"
     CDS             99..293
                     /locus_tag="SCM56_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110515.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            562..861
                     /locus_tag="SCM56_001736"
     CDS             562..861
                     /locus_tag="SCM56_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110516.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(958..1392)
                     /locus_tag="SCM56_001737"
     CDS             complement(958..1392)
                     /locus_tag="SCM56_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1110517.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1624..2334)
                     /locus_tag="SCM56_001738"
     CDS             complement(1624..2334)
                     /locus_tag="SCM56_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEG1110518.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2324..3184)
                     /locus_tag="SCM56_001739"
     CDS             complement(2324..3184)
                     /locus_tag="SCM56_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEG1110519.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3189..3827)
                     /locus_tag="SCM56_001740"
     CDS             complement(3189..3827)
                     /locus_tag="SCM56_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEG1110520.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3842..4141)
                     /locus_tag="SCM56_001741"
     CDS             complement(3842..4141)
                     /locus_tag="SCM56_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1110521.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4184..5668)
                     /locus_tag="SCM56_001742"
     CDS             complement(4184..5668)
                     /locus_tag="SCM56_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEG1110522.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5688..6149)
                     /locus_tag="SCM56_001743"
     CDS             complement(5688..6149)
                     /locus_tag="SCM56_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1110523.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6167..7234)
                     /gene="ulaG"
                     /locus_tag="SCM56_001744"
     CDS             complement(6167..7234)
                     /gene="ulaG"
                     /locus_tag="SCM56_001744"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEG1110524.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7452..8222
                     /locus_tag="SCM56_001745"
     CDS             7452..8222
                     /locus_tag="SCM56_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEG1110525.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8495..9322)
                     /locus_tag="SCM56_001746"
     CDS             complement(8495..9322)
                     /locus_tag="SCM56_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1110526.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9890..11125
                     /locus_tag="SCM56_001747"
     CDS             9890..11125
                     /locus_tag="SCM56_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEG1110527.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12124..12384)
                     /locus_tag="SCM56_001748"
     CDS             complement(12124..12384)
                     /locus_tag="SCM56_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEG1110528.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12411..13712)
                     /locus_tag="SCM56_001749"
     CDS             complement(12411..13712)
                     /locus_tag="SCM56_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1110529.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14004..14462)
                     /locus_tag="SCM56_001750"
     CDS             complement(14004..14462)
                     /locus_tag="SCM56_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1110530.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14475..15806)
                     /locus_tag="SCM56_001751"
     CDS             complement(14475..15806)
                     /locus_tag="SCM56_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HEG1110531.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15817..17259)
                     /locus_tag="SCM56_001752"
     CDS             complement(15817..17259)
                     /locus_tag="SCM56_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1110532.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(17246..18010)
                     /locus_tag="SCM56_001753"
     CDS             complement(17246..18010)
                     /locus_tag="SCM56_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HEG1110533.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18114..18989)
                     /locus_tag="SCM56_001754"
     CDS             complement(18114..18989)
                     /locus_tag="SCM56_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG1110534.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19244..19328)
                     /locus_tag="SCM56_001755"
     tRNA            complement(19244..19328)
                     /locus_tag="SCM56_001755"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19292..19294),aa:Leu,seq:caa)
     gene            complement(19346..19417)
                     /locus_tag="SCM56_001756"
     tRNA            complement(19346..19417)
                     /locus_tag="SCM56_001756"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19383..19385),aa:Gln,seq:ttg)
     gene            complement(19427..19499)
                     /locus_tag="SCM56_001757"
     tRNA            complement(19427..19499)
                     /locus_tag="SCM56_001757"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19464..19466),aa:His,seq:gtg)
     gene            complement(19561..19645)
                     /locus_tag="SCM56_001758"
     tRNA            complement(19561..19645)
                     /locus_tag="SCM56_001758"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19609..19611),aa:Leu,seq:caa)
     gene            complement(19666..19736)
                     /locus_tag="SCM56_001759"
     tRNA            complement(19666..19736)
                     /locus_tag="SCM56_001759"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19702..19704),aa:Cys,seq:gca)
     gene            complement(19782..19853)
                     /locus_tag="SCM56_001760"
     tRNA            complement(19782..19853)
                     /locus_tag="SCM56_001760"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19819..19821),aa:Gln,seq:ttg)
     gene            complement(19865..19937)
                     /locus_tag="SCM56_001761"
     tRNA            complement(19865..19937)
                     /locus_tag="SCM56_001761"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19902..19904),aa:His,seq:gtg)
     gene            complement(19940..20013)
                     /locus_tag="SCM56_001762"
     tRNA            complement(19940..20013)
                     /locus_tag="SCM56_001762"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19979..19981),aa:Trp,seq:cca)
     gene            complement(20020..20100)
                     /locus_tag="SCM56_001763"
     tRNA            complement(20020..20100)
                     /locus_tag="SCM56_001763"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20064..20066),aa:Tyr,seq:gta)
     gene            complement(20102..20177)
                     /locus_tag="SCM56_001764"
     tRNA            complement(20102..20177)
                     /locus_tag="SCM56_001764"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20142..20144),aa:Phe,seq:gaa)
     gene            complement(20213..20285)
                     /locus_tag="SCM56_001765"
     tRNA            complement(20213..20285)
                     /locus_tag="SCM56_001765"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20250..20252),aa:Asp,seq:gtc)
     gene            complement(20295..20367)
                     /locus_tag="SCM56_001766"
     tRNA            complement(20295..20367)
                     /locus_tag="SCM56_001766"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20332..20334),aa:Val,seq:tac)
     gene            complement(20383..20454)
                     /locus_tag="SCM56_001767"
     tRNA            complement(20383..20454)
                     /locus_tag="SCM56_001767"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20419..20421),aa:Glu,seq:ttc)
     gene            complement(20469..20558)
                     /locus_tag="SCM56_001768"
     tRNA            complement(20469..20558)
                     /locus_tag="SCM56_001768"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20522..20524),aa:Ser,seq:gga)
     gene            complement(20598..20671)
                     /locus_tag="SCM56_001769"
     tRNA            complement(20598..20671)
                     /locus_tag="SCM56_001769"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20635..20637),aa:Asn,seq:gtt)
BASE COUNT         6528 a         4335 c         3285 g         6530 t
ORIGIN      
        1 tgattctttt tatttatcat aaagatatag atacataatc attttcactt acatatattt
       61 gaatattaac taaaaacagc aagaaagaag aggatcaaat gtattatgta gaagtaaaaa
      121 caaaaggcgt caaaaacaaa caatatgtga aaggtatcag taacgaatat cctttacttg
      181 gttcatggaa agaagctgct cctttttcca aaccatgtgc cataaaaata aagaacgagc
      241 tagaaaaaga actgacttgt ggtaaagctg ttgtagatat catcgaaaag taaatccaat
      301 gtatagaaag gaagatcaca catgaaaagc gacgtacaaa ttgctaagat tatagaatta
      361 gaagaagaag acgcagtgtt gacaattgaa aaaaaataaa tgtttgtata ccatatgatt
      421 atttcaatat gtatcctgga attggagatc tcgtagagct atatcaagat gaatcaaata
      481 tacttgtcat tcatcatgaa atatagagac ttataaaatt gtttattttt ataatgtatc
      541 agtatagaaa ggaaataaaa catggattat actgttgaat ttgacagtga actacttaag
      601 caatttccac aagaagaaca tttcatgtat aaagaagcaa tttcccacaa agtgacagag
      661 tatttggagc ataatttttt tcgtataaag ccagtgcgta ctcgttcggc aaaaggaata
      721 tacgagatga aagtcaaaat taataaaaaa aattatcgca tagcatttgc tttaaaaaaa
      781 aatcatgtgt tcatttttta tattagttcg aatttgcaaa aaattgtatt tgataaagag
      841 gtgagtaaaa aaataaaata gctgagcatt ggaggataga ttgagtgata ctaagtctat
      901 cctcttgttt ttctaaagaa ctaagctaga attttttctt ttgtacgtta ttatatgtta
      961 tttgtcatta ccaaaaagca ccttttttct tctagtatct atgacataag aaataccacc
     1021 catttccttt acttgaacaa atcctactga ttcataaaat ttttttgttt tctcagtatc
     1081 atctgtcaat aattcgattt ggactggtcc ataactagtg atcttctcca aagcttcatt
     1141 caaaagcact ttgcctattc cttggcgctg atacctcgga tgaatgagga tatcttgaat
     1201 aaatgcgatc gtcgcaaaat cagtgatcca acgaacgatt ccgataatct tcccttcccc
     1261 attacgcttt atcagagatt cagattgttc aaatgctcta gctaaccttg cagtgtcttt
     1321 tgtgtaattc gtccaaccaa cgctcttata taattcagaa atctcatcga tcataaggga
     1381 atcaatattc atttttttct tcctttctta tacaattctc cctctaataa tacagttctt
     1441 tgactattta atctatcaca tataactctc ctgtaaagat tctctcgatt ccttaaaatt
     1501 cctctttcct tttagaatgc atactttttg ataaataaaa aggttgtgat aaattttctt
     1561 cacaacctct gtttctctct atcaagtgtt atcttttaat tttctaccct taccctagca
     1621 tctctattgc ccgtagtatg catactttcc atgtttgcga aaataatgtt tatctagtaa
     1681 atattgcggg atttcacata tattggggtt gatcagctcg ttttctttgg ccatcagaca
     1741 aatttcatct aagatcaaac tgttttctac tgctttcata ggtgaatccc cccatgtgaa
     1801 cggtccatgt ccatagacaa gtactccagg gacttcgttt ggatctaatt ttctttcctt
     1861 aaacgtctcg acaatcacat tccctgtatg aacttcgtaa gcttctctta cttcttcttt
     1921 cgttaattgg cgtgtacaag gaaccttacc ataaaaagca tcagcatgag tcgtgccata
     1981 agctggcaaa tcgcgtcctg cttgcgccca catgacggag tgcgtggaat gagtatgagt
     2041 aatagcagtt atatcttcaa aagtttgata caaaacaaca tgtgtgggga gatctgaaga
     2101 tggttttaag ctatcttctt ctattatatt tcccgacaaa tctgttacca ccatttgatc
     2161 tgcttgcatg cattcatatc tgacaccgct tggcttaatg acgatgactc ctagctcacg
     2221 gtccacttca ctcacattgc cccaagtcaa tttgactaat ccgtatcgcg gcaatgccag
     2281 atttgcttca aggacacgtt gcttcattgc tgcaattttt tcttcacaag acatcatagc
     2341 cagcctcctt caatttagaa cttaagtagt cttttgcctg ctggatttct cttcgaaagt
     2401 cttggctttt ttcactccac atttcaatca gaaatgttcc atcataatct agacgtttca
     2461 aatgtcttaa taatccaaga aaatcaacac aaccgctacc aaaaggaaca tcacgaaact
     2521 ggcctgaata ttcttttgtt acagggtaag tatcttttaa gtggatagca gtgatataat
     2581 cgatcccttt ctcaagttct cgagccggat tattttccgg ccatgcggac aaattgccta
     2641 ggtctggata tacttgtaga aaaggtgaat gaatctgttc tttgatttct aaaaattttg
     2701 aaatggagtt cataaatgga tcatccatga tttccacgga aagaatgatg ccatatttag
     2761 aagcttcctt tacgctttct tttattcctt cgataaagtc ttctctggaa ttcattgatt
     2821 tctcttcata ataaacatcg tatccagcta tttggattac tttgacgctt agattatgcg
     2881 ccaaatggat tgcttttttc attagttgtt tcgctgcttt tttcttttct ggatcagcag
     2941 aaccaaacgg aaatcgccga tgcgcactta aacaaatcga atggatacgt acaccggagc
     3001 tgaacatctc ttctctcagc tcagctatct tttcttcaga ccaatctaat cgtgctaacc
     3061 gctcatccgt ctcatcaatc gacatctcga taaaatcgaa gtccatttct tttacaaggg
     3121 aaaaacgttc tttccatgaa atatctttgg gtaacgcttt ttcataaatc ccaatttgag
     3181 ccatttcctt acccccagat acgaatgatc tcatctttga agttttgtgc ggccttcatc
     3241 ggatcatttg ctgctgtaat accacgacca gtgataaagg tatacacatc gatcccttca
     3301 aataagtgta atgtgtgtgg atttaatcca ccagttacag aaacattaaa gcctagttca
     3361 atcagttttt tgatcttcga taaatctttt tcaccccaag tttcaccaga taacagcgca
     3421 tcacggcttt gatggtagat tacctgccgg ataccgatgt tatgccaatc catggcttgt
     3481 tcaaaagtcc aattgccgta gagttctact tgcaattcac ctatttcttt ttgtgctgcc
     3541 tccatagttg gaatcgttgc acaacaaatg actgtcatcc agtctgctcc tgcttttgca
     3601 acattactcg ccactgttcc tccagcgtct gcacatttcg tatcagcgac tagtttttta
     3661 tctgggaaca tactgcgaat acagcgaatc gctttttgtc cttcctgtaa gcaaagaatt
     3721 gtgcccactt caataatatc tactacatca ccaactttca taacatctcc taatgcatgt
     3781 tctaagtcat tgtgatctaa cgcaatttgc aaattcggcc tgctcatctt agttccttcc
     3841 tttacttgtt tttttggtac cactttgttt cagcaaattt ttcactgatt tctttgactg
     3901 acatcacatt tttgataccg ataactgtaa ctcctttttt ctctgcctga gtaaacatat
     3961 tcaaaaaatt gactggcgta aaaactacat cgtattggcc agctgaactt tttccctctg
     4021 aaatggcaca atgatggatt ttggtaattg gtacgtttag ttctttcatt gctttttcca
     4081 cacttctcat catcatcaag cttgttcctg atccatttgc acacgatact aaaattctca
     4141 tcttaatccc tccattatga gtgttgtcta atcagatttt cttttatact ctctgctttt
     4201 ctgcatatgt gtcatagtct tctacaatca aaaaataacc atcaggattt ttccgatatt
     4261 gtaactgcgg aatagccaat aaaccaatga caactacggc aattccagca taacctagga
     4321 ctttcatgat tactgtaaac aatggccaag ctgttgccca gtcgaacatc ccgatataac
     4381 caccgtatgt tgacatccca atccatgtag cgaataatgc tgaaccgcct acttgtatga
     4441 ttcctgaaag aaacgggaac aggcaagcgg ctttgaatcc acctcggtta ttggcataaa
     4501 ctgctataac ggcattatca aagaacaacg gaatgaaacc ggctatgacg atcgttggtg
     4561 atttcaataa aatcagtaaa ccaatcatca aaaactgtcc caaagcacca aacaagaacc
     4621 caatcgttac cgcatttggc gaaccaaagc caaaagtagc agctacgtca attcctggaa
     4681 cagctcctgg taaaatcgtg tttgagattc cttggaaaga ttgagttagt tcatcgacga
     4741 atgtccgaac acctaattgc aagatcgcca gatataccgc aaaatttaaa gaagtcgtca
     4801 taatgtagaa taagaaactc tgcccttctt gcatgaattg cgcttgaatc aaataatctt
     4861 tgcctagtac aatcagaatg atcccaaaga aaaatagcat gagtaacgaa gtagcaacca
     4921 tattctcatt gaaaatcgac aagaatcctg gtaatttgat atcttcaatt tttttgtctt
     4981 gcttgattga tctatttttc ttttcatccc gctttttcat ccactcagct aattttgcaa
     5041 atataaagat tccgaacatt tgctgatgtg ccactgcaaa tccggcacct tctgtcaatt
     5101 cctgcgtgat tcctactgtt aagttagaag ctactgtcca ataacaacct aaaatcagtc
     5161 ccataaaaat caatgcttgt atcctaccta gatcaggaaa gcaaaacagt aaaatccaaa
     5221 aagcggtagc tgcttgttga acctgaacgt ttccggtagt gaaaacggaa cgcagtttcg
     5281 tatatttctg aaaacggact aataagatat ttaaaacaaa tgcaatcagt aataaaatca
     5341 tcacatctcc aaaggtacgg ccaaatgtct ccattaatcc agcatccaca gcattttgtc
     5401 caaaataagg atctgtaacc atggcatcca aattgaaccg ttcctttagt ccaaccaaga
     5461 ttggccgaaa attattgacc aaaccaccag agccaacagt caaaataaag taaccgaccg
     5521 ttgcctttaa gaatccagcg atgcattcat gaagcgggcg tttcagcaaa atatagccta
     5581 ataacacgat aaaaccaatc aggtatgctg gctgcgtcaa gatatttgtt gcaaaatacg
     5641 tccaaatgct taacaaaaat tcccccatct tttttctcct ctatcaatta attatattct
     5701 gccatcacaa cttggtaatc atcgatggag ttcgtagaga tgagacgctc aaccaatcca
     5761 tctgtcatta ataattcgga taatttttgg atattttcta gatgttcttc tggatctttt
     5821 gctgcgagtg taaaaaacaa actagccgtt ttctcctcat tctccccttc actttcaaaa
     5881 cggattggct gtttcaactt ggtaaatgaa atagctgttc caaaaacacc atcacttttt
     5941 tctgaagaat gcggcattgc tacttgaggt gcgatcacaa tataaggacc gtatttttct
     6001 acacaatgga tgatctcctc cacatacgtt tcatcaataa tttccttatc gatcaataca
     6061 agacagcttt cggcgatagc ttcacgccaa tccgtgattt ctttatctgg gtaatgaatc
     6121 aattgatggt caaaaaagta tttcagcaag aaactcccct tctttcttat aaaaacgacg
     6181 gaaacggtgt atcattttca atcgagaaga catcttcaaa tcctcgataa aaattaaact
     6241 caagatcatc tttgtttgtc ggatagatga atttcccgcc tacttgccag atataaggtt
     6301 taaatccata cttcaggcga tcctttttga atttccaaat ttcagtgatc tcttttggat
     6361 cagccataaa attagaccaa atatcgtagt ggacaggaat cactactttt gcattcaatg
     6421 actccgccat gcgcagcata tcaacagaag taactttgtc tgttatccct cgtgggtttt
     6481 caccataggc ccctaaacag acatcgattt tgtgttcatt accatgtttg gcaaataaat
     6541 tggagtaatg tgaatctcct gcatgataaa gattccctcc tgatgtttcg aataaatagt
     6601 ttactgcaat ttgatccatc tcctgaggca tttttccttt taatacctct tctggatctt
     6661 cacaagtgac caacgctgtc cgatcaaacg cttctaaagc tactacattg atgtctttga
     6721 ttttgactgc atcacctggc tttactgtaa tcgttttttc ttcaggaatt ccccattttt
     6781 gccacgtgcg aaccacttct tttggcccaa tgaatgttgc ttcaggacaa ttttgatgaa
     6841 ctgctgcggc tgtatggata tctagatgat cggaatggat atgtgtaacg attaaagcgt
     6901 ctacattttt tatcgcaaaa ggatcgatta cgaaaggctg agtacgtaaa ttaggctgca
     6961 tattttgaac gccactcata cgcatcatct gatggccttt tttcatcatg ccattcccat
     7021 gactacgttt tcccgtccca caccatagat cacataaaat attcgttcct tcatgacttt
     7081 tcagccaaat tcccgtacag cccagccacc acatagcaaa agttccagga tccactactt
     7141 ctgtttcgat ttcttcattc aagtatgtgc cccactcagg aaatgtttct actaaccact
     7201 tttcttttgt tacttcattg acagttttac tcatttgctt gctcccccca tatcgttgtt
     7261 ccaacttaca agtaaatatt aatgtgtatc agcatccatg aaaacccttt tatatgaccg
     7321 tttgtttttt atatgatttt ctttcagtaa tcaatgattg tttcagtata ataaagacag
     7381 atatgaatgt ttaaatgaac aactagtcaa ataaatccat ggatcaagat gtccctcagg
     7441 aggcaaaagt catgaaaaac tcacttgtca atattgaaaa acgtcaaaaa gaaattttgg
     7501 aactgcttca gcagactgaa caactatcaa ctttcgaatt agctgaatcc cttgatgtat
     7561 ctattagtac gatacgaaga gatttaaatt tattagaaga aaaaaacgag atcatacgca
     7621 attacggtta ttgttcttat aattacaaaa accagactga ttttgatcaa tctggtcctg
     7681 aaaggattaa acaagcgatt gccagagaag ctagtaaata cattaacgat tatgatacgc
     7741 tgtttatcaa ttctagctcg actgcgttga aaacactgga ttttcttcaa tctaaccatt
     7801 tgacagtcat tacaaacaac gtgaaaatca attactctcc gcacaaagat aattgtagtt
     7861 atattttaac tggtggagag atgcgctttc caaaagaagc actagtgggc gacatcgcat
     7921 taaatacgat ttcttcagtc aatgcagaca tttgtatcat tggctgtagc ggagtcgatg
     7981 tagtacatgg ggtcacaaca aagatattaa atgaatcaac gatcaacgaa atgatggtca
     8041 acaagacaat caaatgtaag attttagtcg ctgatcatcg aaaaatcgga ctgacatcaa
     8101 agttcaagat tgctgatatt tcagtctttg attatttgat cactgataaa ttttgttctg
     8161 aaaaagtatt agaggagatc agaaatacgg ggataaaaac cattcaagta gattcatttt
     8221 aagaaccagg acgtaaatat gtaaatagga ttatcaaaaa aatgaataac aaatttatcg
     8281 ataaatgaaa aacacgctac gctcaaattg ataaaggaat cccaagatgt atccatccaa
     8341 tcgataccat cataatctat tgcttctttt ataaccgtta tatgttgcct aaataggaat
     8401 caaacaatat cgaaaaaagg aggtatgaca aaagtcacac ctcctttttt cgaataaacg
     8461 gtattcaaat cagaacactg atttcacaac cactttattt caggtcgttt tcaatcacct
     8521 ttaccgcgta atcatttccg ttgaaacgcg ctattttttt tgcgtccata tatctttcta
     8581 aataatcatc ttctcctaat tgcttatggc aagaagcaat ttcataaagc aagtattcca
     8641 atgcataagt cgatttatgt ttcttattat actcaatacc cttctcacat aattggattg
     8701 ctttatcata atcctcgatt ttacggtaat aggtggcgat atcataatat acaatgcaga
     8761 ccatcagatt atcaaaatca gtctcactca atattttaag tgtacgctcc acataaactt
     8821 tcgcaaaatc taattctcct tttgacaagt agatcattgc cacgtttgca gctgcttgta
     8881 agtgatggat atctgtttcg gtcgtttgtt ccaacacttg attaaaataa aataatgctg
     8941 catctggttt tttcgcataa agatgatcaa gcagtccttg ataaaataaa atttgattta
     9001 aaagatcaga attacttact ttttcctgat cgatcgtttg taaaatttta taagattctt
     9061 ggtattttga tacagaattc aatgattgaa tctcatttat ttttttcctg attttgtctt
     9121 cttctgtttc tactaatata tcggagatct cgacattcag ctttttgcaa agcgcactta
     9181 agatattgat gcctggcgac atattcttgt tttcgatatt gctgacagtt gcttgttcac
     9241 aaatgccttc tgctaattgt acttgtgtta accccttttt ctttcggtat tcttttagta
     9301 atgctccatc tactttaatc aaaacaattt ccttcccttt aaaaaattat ttctccttta
     9361 tatcatacac aaaaaaaagt tctaaacaac caaaaatgtt tagaacagaa taaaaatttt
     9421 aattttaaaa taatattcaa attaaacaac tggcgtatct gctccttgat ttctttttgt
     9481 gttatctgaa taattatcgt ttacagttat aacacatagt gaaaattgtc atggcgagaa
     9541 agtctaagaa tatttttaaa cataactatt tctttcttac gtgtgtataa tgacgacagc
     9601 acacctatca attaatataa ttattttatt tttttatgtt ctcatgttta tttttaatga
     9661 cacaacttct cgtatttatc tggcacaaca tataaatcga tcaaaaaatt gttgcgtgtt
     9721 tttttgaaaa ttggagtttc aacttgatta caaaatttaa atttagaagt tgtgaaaaca
     9781 gcgagagaca gtttaatatt caaaatatac ataggctact cgaacgctgt gttttttagt
     9841 atactagtaa gaaacgaagg gatagactca ttccaataag gaggatttca tgaagacaat
     9901 tctttttgct cctgcagtat tcaatttagc tgagacaaca cgaatgattg aggttgccaa
     9961 agctatgaaa gaccgttatt actgtgtatt ctttggcttt tctaaaactt ttgctccttt
    10021 gatcacagaa agtggctttg agtatcattt actagcgccg atccttacga aaaaacaaga
    10081 agaacaaatc ataaaatttg accagttgcg tacacttaaa aaccctttta ccaaagaaat
    10141 ggttgcctct cgtatcacta gtgaacagag tctgattgct aagtttgagc ctcgactgat
    10201 tgtgacaggt tcgaatgtga caattttttt atcggcccgc aataaaaaga ttcctttagt
    10261 atatgttaaa ccctatgctt tgagtgaacc tttttttcaa tcagagcatg cgctgatgcc
    10321 ccacgagtta cgtttcttcg gccctttaag caatcccctt tggcactgga caaaaaagcg
    10381 tatagtaaat acccgatgga tccccaatgc cttcaaacac gctgcacaaa aagaaatgct
    10441 tcctttacca aaaacaagtt tacaaatgat ggatgctgat ctaaatctca tcacaacgcc
    10501 taaactattt ttggaagaag aacagctgcc ggaaaattat cgaatcgttg gtccaatttt
    10561 tgctaagctc agtacttcct tgcctgaaga agtgctaact tttatacagc gaaagcaaca
    10621 agaaagaaaa cgtattatct atttcgcaat gggtagttca ggcaatccgc gtatgttacg
    10681 caaaattttg ttcttcttac aaaaacaacc ggatctagcg gttattgcac ctattgacta
    10741 tctgcttgat gacattcaat ctgactcaga agatctttta ataacagaat atctgtctag
    10801 tcatcttttg catgaattca ttgatttttc ttttatccac ggcggggaag ggaccgtcca
    10861 aactgcctgt gaatccggca aaccgtttat cggtgtgggg atgcaatttg agcaaagctg
    10921 taatattgac tattgtgtaa aatacggaaa cgcaatcgcc ctcacaaaac cagtgactga
    10981 aagaaaactg cagattgctc tgcgcgaagt ttcttcaccc aagattcgtc agcaggcacg
    11041 cacgctgcaa gccgtttttc caataaatgg tccacaacaa gcaattcaag aaatcgaaac
    11101 cttcctgagc aaccacccaa tatagtttca agtagaaaaa gcttttttaa tagagactca
    11161 tcagtaattt cgctattcga gacccagaca gtgaaaatac aaaaaaagta aatagtggat
    11221 tgaaaagtta gagttactta aattacggtg tctaaaaaac acaaaatcat ccttaaaata
    11281 aaagatctga cgctatccaa atgagcatag gtagcgtctg ccatttattc ttagtcaaat
    11341 tctttgacac ctttctctgg ttggatcatc tcaacgtttc ttcaaaagaa agaccataat
    11401 aattaggcaa atacattttc ttaaaatgtg tcatatagat ctagaagcat cgctttgtag
    11461 agatttgtgc cgctagtccc ctcaacaatt actaaatcaa atgtaggatt taccacttta
    11521 tctttgataa tcaacctccc tcttctgttg agacaatgat ctcgcaatcc gatgcatact
    11581 cctatataaa ctgccagcaa tccccacata ttgagtagat catgtcagaa tgttctatgt
    11641 cgtcagcaac gatacactaa aagtcacgtt caccatgact gattgctatt cccagcacaa
    11701 ttatttctat acacgtttca tgatttgaat aaacactcac atcatacata ttaccattca
    11761 tacaccatac tgcagcccct actatatggt tgtaagctcc caaatctata tttgacttga
    11821 tcactttggt agtagatcta ctaattctaa atccattgct gataaaattt ccattttttc
    11881 ctccttcttt ctgccaaaac agttaaaaca ataataatct ttatgtaaaa aagggctgtg
    11941 acaaaagcct tgtcacagtc ccatattccg aataaacggt ggtcaaaagc ttgcatctgc
    12001 ggtaataagt tcaaccaaac gaaaaataag ccaacatttc acaaaaattt aagaagcaat
    12061 tttcgtaaat ttcttcttat ttcttgaagc taaccacttc tgttacaacc acttttagaa
    12121 catttaaagc ttcgatgtga caaaccatct atgctgctcg atcataaaat tgatagcaaa
    12181 aattccacaa agtacatatg tcccccatga aaacaaaaca ttcatatgcg gtacaatgat
    12241 ctttggaaga atcacgatca aaaatgatgc cagtgcccaa atccatcgat tgatttgaat
    12301 atgatatgta gctagcatat tcaatataag gtgactagcc gttaataaaa agataaaaac
    12361 aaaaaataaa atcagccaat acattgtttc ccaccttcta aaaaccaaac ttagacagtc
    12421 tggacttcgt tttctccacg actttgttca tctgacagca tctttttatc ataagctttt
    12481 acaaatggat aatagataat aaatgctatc aatgtgttga tcatgatcaa gataacaaca
    12541 cgccagtcat ttcctgctga aagataaccg ccgattggag ctggtaatgt ccatgttggc
    12601 aaaacagtaa atctattgac catttctaat ttgactgcaa aataactgat aatagtcaaa
    12661 actaaaggaa ctaagttgaa cgggatgaac atgtaaggat tcaacatcat aggagctcca
    12721 aaaattatgg gctcattgat attgaagata ccaggaataa tagaaaaacg accaacctct
    12781 ttcaagaatt ttgatttaca gaagaatata aacaaaacag ataccacgat cgttgctcct
    12841 gcaccaccaa ttgttaccgt ccattgataa aactgttcag gagcgatata gggtaattgg
    12901 gtggcagctg taccatctgc caatgccttt gcattctcat ccaacatgat cagccacatt
    12961 ggacgaacaa cagcccctac aatactcatg ccatggatac caaatgacca caacaaatgg
    13021 atcaaaaaca ttggtagtaa aacaccaagc aaattattcc ctaaaatatt ggttaaaggc
    13081 tcgaataacg ataataaggc agcgtttaca tcaaagcccg cgacgcttcg aatcagccaa
    13141 gtaattgtta aaatgactgc acctggaatc aatgcctcaa aactacgggc aacagcaggt
    13201 ggcacttgtt ctggcatttt gatagtaata tttcgatttt taaagaaacg atatacttcc
    13261 actgcaaaaa tcatagataa gatccccgca aacatcccag atgctcccaa agaactcata
    13321 ggtaagaccc agccaagagg tgcctcttca ggagttaaaa cgtttacagg aacttgtaac
    13381 ataaagaacg ttcctaaaga taatatacca cctgtcacac tatctaatcc atatgattta
    13441 gctagatccg aacccataac aaaagttgca taaatagcca tcaatcccat cgtcatccta
    13501 aagggtaaaa caatcgttgc cgaataaggg gcaataaatt cattccatcc aggaaacggt
    13561 aaattgataa agaccataaa aaatgtccca atcaaaatca atgacaatgt tgaaacaacc
    13621 ccattacgaa ttgctaataa atgacgctgt gtaccaattt ttgccattgg tgggacaagt
    13681 ttctcttcga cccatttcat cagcttttcc actaaaacca ctccttttca ataaaatatg
    13741 aaccattcat tgcatgacaa cgcaaagaat gttcataact aattatataa ttaagaaatt
    13801 ttatttcaat atattctctt aaataatcaa atttattttt aagaatcctg aaaataagtt
    13861 ttctaaatca taggagcaac aaaaaaattc gataattaac aaaaatcaaa atgattaaat
    13921 ctagtgattg aaacatatca tttcaaaaaa attttgtaaa ttcacccata aatatcaccc
    13981 tatttaactt ttttcatcgt acactaaaat tcaaaacaac tatctttaat ctttacttct
    14041 tggttttgtt ttaataaatt tccttcaaaa atgcctggag aaactacaat gatcaccgta
    14101 tctttttgtg cttgctccaa cttcttcaaa tctataatag ctaacagtgt cccttctttt
    14161 acctcctggt tactttcaac ccgtatctga aacggacttc cttcgagttc aactgtatct
    14221 aaacccatat ggatcagtat ttttctgcca gattcacttt cgatcgttat ggcatgtcgg
    14281 gtaggaaata tattatcgat tattccttta actggcgcat ggatatatcc atcatgattc
    14341 gttatagcgt atccaactcc tatcattttt gaagaaaaga cctcgtcttt tactgctgtt
    14401 aatggaataa tttgaccact agctacagct tttagaatgt ttcttgtttt aaagaaatgc
    14461 actttttatc acctctactt taatgctggc caatattttt tgtttacttt gatcatttca
    14521 tctagtattt tttttgcgac tctggcattt ggtacggttt gattcaatgt aaatgcttgt
    14581 aaagcttttt gataagaacc ttcaaagtaa gcatctacta gtaacttctc tactgcaact
    14641 tgtgcctcca tcaatccttt atgaaaatca ctgattggtt ttcttagtgt aattggctcc
    14701 acaccagttg caccaacata tgccggtatt tcaacaactg catctgaccg tagattcgga
    14761 atcgcccctt gattaggaac gattagcata aagcgacgac gatcatcatt caacaaggaa
    14821 atagctatat cgacgatata ttgtccatgc tccccaaaat taaagttcaa tacttcttcc
    14881 acaataccgc ggcgtatttt atcagccatt tctttggtat ttttctctcg tccatccatg
    14941 accatattag cacgtgtata ctctttgtca gtatgctcaa cgaccatatc aggatataag
    15001 taatactcta agtaattatt cgggatatta cttggaaaat ttttcgtgat gactgacatc
    15061 atttgaaagg ttttttgcca agttttatct cctatattaa aatcggcgac ttgcatttct
    15121 tttgttatta gtttttcaat aatctctggc ataatttctc gttgcaattc tttatcataa
    15181 atcgatgtat accagccaaa gtggtttaaa ccataatacg taacgatcca attcttacga
    15241 tcataaccgt aattcactgc aatcgtttct tcaattgaaa tagtcatatc acaggcatta
    15301 atcattttta cattcggata tttacgacga acagcttcag aaacgatcga ctctggattt
    15361 gtataattca aaatccatgc tttgggggca tagtcttgta tatgatcgac aatttccaat
    15421 aaacctttga ttgagcgcat cccataagca aatcctccga gtccacacgt ttcttgtcct
    15481 actaatccat gtttaagtgg aatcttttcg tccttttctc gcatctccaa tcctccaaca
    15541 cgaatttgtg agaaaacaaa atcgcatcct gtaaaagcaa gctttggatc atccgttgca
    15601 cgaatgcgaa tatttgaaaa accttttctt ttcagcataa atttaacaat caagtacata
    15661 tccatatttc tttcttcatc aatatcatat aaacgtattt cagataatgg caaccgtttt
    15721 tcattgttca aaatggcttg aacgattcct ggtgtatacg tactcccgcc tccagctata
    15781 gtgataatct gttttttcat catcatcttt tcctttctat tcttctaata atttattgaa
    15841 ttctgccata acataagtca cttctaagcc gatgataatt tgataatttt tttcagttgg
    15901 tttaatgatc ccgcttatcc ctaattgctt gattcgctgt tcctcgacca tggtgggatc
    15961 cactacctct acccgtaggc gtgtattaca actatagaca tccaggatat tggcttttcc
    16021 tccaaacgct tgtagcattt ttgttgccat atattcataa tttttacttg ctaaccctaa
    16081 ctctgtttct tctaaggtta cttcattgcc aaactctgcc ttagcttcac gtcctaatgt
    16141 ctgaatgttt ctcttattga tcaaataata aaaagtaacg taatacaagg caaaaaagaa
    16201 aattccgatt ggaagaatca aaatagcatt ggtagcgatt cgaaaattga tgagataatc
    16261 aacaatacat gctccgaatg taaaaccaat tcgtatattg aataagtaac agacaatccc
    16321 tgctaaaccg gcaaaaagtg catggatcac gtacaacata ggagaaagaa acatgaaact
    16381 aaattcaaca ggctcagtga tatttgcaat aaatgaagtt cctgcaccgc ttcccatgat
    16441 tcctttgacc atctctttat tttctttata agctgcttta tagatagcta gacatatagc
    16501 tggtatacca aacatcatta ctacaaaaaa aactgataaa aacagtccag cctgaggatc
    16561 tccgttgata aagcgtgtca tctctcctct gaaaacttcc ttgctaggag atgtatagct
    16621 tcctaaatcg taataaatat acgtattcaa cacatggtgc agtccaaagg ggatcagcaa
    16681 acgattcaaa aacataaata tccccacacc taatgctccc atatcaacga gcagttttgc
    16741 aaaattatca atcccaattt ggatgagcgg ccagataaag cccaaaaaaa cagctgcgat
    16801 tgtttgtaga atcatcacca tagtcaatgg gaatttttcg cccgcaaaat aagaaaaaac
    16861 tactggtaat ttttgttctt taaagcgatt atacgtccat gccgctacta aaccggaaat
    16921 aattccacca aaaatcccca tattcacttc tgggtccata atttctaagc cttttttcaa
    16981 ggtagcaatc gctaaaaaac cagtaagtgc aggtattcct ttgtccttat ttttgacaaa
    17041 accgattgca actcctatag caaataagat atccaagttt ccaaaaacgg catttccagc
    17101 tgcaccaatt actgggatgt tcagcatatc gtcagctgat aaacgcatga tcaatcccgc
    17161 taatggcatc gctgcaactg gaagtagcat cgctcttcca agcttctgca catttgattt
    17221 aacattcatt tttattcctc ctctattaaa tatcctcata tgttactaac tcaatcaaag
    17281 gatcattttt cactttttga gcaaatgctg aatgaattaa aaattcgact tggtgggtac
    17341 gaggatacac aaaacttgaa ccatttaaca attcctgatc caaatacgct gcgtgacaca
    17401 tgacttcgat cgttttatct tctctcaata tcatgagtaa acttgccaaa tattcttcca
    17461 cactcgtatt gatttgtcgt tttttttcat cagtttctcc tacgacatcg aaacaaggct
    17521 caaaataatc gacatgacga acttcatctt ttcgttcaaa gttgctccga accggcaaat
    17581 catatttttt tgctaatgta acaactacct cctgattttc atccaaagta tgagtatgat
    17641 gatgactgtc taaatgagtt ggtatgatcc ccgcatgata aattttttga atctgagcat
    17701 tccattcctg atacagttga tctttgtcaa tgttaaatgg atgctgataa taagataagg
    17761 atctgaactc tccgctttcg ataagagtat cgacagtttt gaggagtggt ttgccacacg
    17821 tcaatgttaa atggactcct acacctagcc taggcatttc ctttcttagt ttcacagcat
    17881 gctcgaaacc cggcatattt gccatcaatg tagttgatgt caaaattcct cgctgataag
    17941 catccataat cccataattc acaccatggc tatagccaaa atcatccgaa ttaaaaatca
    18001 cttttcccat ttcattctcc ttcttttttc tactaaaata ggcgcaacca aataatcata
    18061 gtccatttac tatgtgtcat ttggttgcgc ctatagatag ttacgccctt attttaatag
    18121 attcttgcta gatgaattgc caaatagatg atttcatttt cttgaatggc aagttgaaat
    18181 tgctgttcga tgatatcttt aatctttagt gctgtacaat acgctttgct atgtttaaat
    18241 gtttcagtaa aaaaaccaat attttctact ggcgtttctt cctgataaat caagcgttta
    18301 accaggaatt tgatatgaac cattaagcga tgataagtca gtgattcttt atcataaacg
    18361 tttgaatagg atttttctag atgggacaaa atcgtggtcg tcatttctac tatttttaag
    18421 ctctttaaac tattaggctc tcctagctca gcattaatga tatgcatagt gatgaatcct
    18481 acttcatttg accctaactc ggtttgatat cgatgattga ttcgctcgac cacttctact
    18541 gctatatcat attcatttgg ataaaggaat ctcatttctg gcaacataat tgctgtgaca
    18601 tctctatctt ctctttttcg ttgaatagca aaataaatat gatctgtcaa tgtaatatag
    18661 atatttgaat ctaatggaga ggagatccta gctgttatca gttcaatcac ttcttccgca
    18721 aaatccataa attcgattgg aatctgcgag actatctcac tgaatcgttt gatcttttct
    18781 ttagaatcta atacaaataa tttgtcagca tcctcaacat caaatggctt actcacttta
    18841 gcagaaaagc caatcccttt ccccatcgca attttttcta ttccatcgtc atcgatcaat
    18901 acagcattat gattcagctt tttcatcaca tacaccgtta attcacctcc tctatatgat
    18961 aaaacaaaac aacttgcaga ctccttcata catatttttg tctccaaaca atttttaaag
    19021 acaaaaaacg acctaagaaa gaaccctttc ttaggtcgtg cctaattgaa tagtagcacc
    19081 ctacataatt tacaaaaata gtttaacaca taataaaagc gttatcaata tcttttttac
    19141 tcgttttatg ataatacttg tatcgaattg atttggaggt aattacataa aaacaaaaaa
    19201 aatatagcaa ctctctataa gaaagttgct atattgataa ttataccggc ggccggggtc
    19261 gaaccggcac gtcctcaacg gacactggat tttgagtcca gcgcgtctgc caattccgcc
    19321 acgccggcaa atttaaaaat aataactggg gtagctggat tcgaaccaac gcatgaggga
    19381 gtcaaagtcc cttgccttac cgcttggcta taccccaaga ataaagggcg aatgatggga
    19441 atcgaaccca cgaatgccgg agccacaatc cggtgcgtta accacttcgc cacattcgcc
    19501 atcataaaag atgttattta atttctcaat aaaaaactaa gatgaagaca tcttagtcaa
    19561 taccggcggc cggggtcgaa ccggcacgcc ctcaacgggc actggatttt gagtccagcg
    19621 cgtctgccaa ttccgccacg ccggcaagtt gatttattag tactaaggcg gtaaccggat
    19681 ttgaaccggt gatgaaggtt ttgcagacct ctgccttacc acttggctat accgccatta
    19741 tactattatc aatctataaa ataaagaata aattatagaa actggggtag ctggattcga
    19801 accaacgcat gagggagtca aagtcccttg ccttaccgct tggctatacc ccaagaataa
    19861 aaagggcgaa tgatgggaat cgaacccacg aatgccggag ccacaatccg gtgcgttaac
    19921 cacttcgcca cattcgccat ggcaggggca gcaggaattg aacccacact aacggttttg
    19981 gagaccgctg ttctaccttt aaactatgcc cctataaaat ggaggagagt ggattcgaac
    20041 cactgaaccc taaggaacgg atttacagtc cgtcgcgttt agccacttcg ctactcctcc
    20101 atggtggcgc gggacagaat cgaactgccg acacacggag cttcaatccg ttgctctacc
    20161 aactgagcta ccgcgccata ttttaaaatt attaattgaa taacaagaac gacggttccg
    20221 acgggaatcg aacccgcgat ctcctgcgtg acaggcaggc atgttaaccc ctacaccacg
    20281 gaaccagttc attatggagg ttaacgggat cgaaccgctg accctctgct tgtaaggcag
    20341 atgctctccc agctgagcta aacctccaac attcaattaa aatgacccgt acgggattcg
    20401 aacccgtgtt accgccgtga aagggcggtg tcttaaccgc ttgaccaacg ggccaggtaa
    20461 atttgctacg gagagtaagg gattcgaacc cttgagacag tttggcacca tctacatgat
    20521 ttccaatcat gctccttcgg cctctcggac aactctccag agtgaaagaa gctcaatact
    20581 ctttctcaaa atgagaactc cggcagtagg actcgaacct acgacatcat gattaacagt
    20641 catgcgctac taccaactga gctatgccgg aataacat
//
LOCUS       DARBDV010000053        20485 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000053 DARBDV010000000
VERSION     DARBDV010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.053"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            4..552
                     /gene="hslV"
                     /locus_tag="SCM56_001770"
     CDS             4..552
                     /gene="hslV"
                     /locus_tag="SCM56_001770"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HEG1110535.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            566..1966
                     /gene="hslU"
                     /locus_tag="SCM56_001771"
     CDS             566..1966
                     /gene="hslU"
                     /locus_tag="SCM56_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEG1110536.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1984..2775
                     /gene="codY"
                     /locus_tag="SCM56_001772"
     CDS             1984..2775
                     /gene="codY"
                     /locus_tag="SCM56_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEG1110537.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2857..3732
                     /locus_tag="SCM56_001773"
     CDS             2857..3732
                     /locus_tag="SCM56_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEG1110538.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="SCM56_001774"
     CDS             complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="SCM56_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEG1110539.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4629..5063
                     /locus_tag="SCM56_001775"
     CDS             4629..5063
                     /locus_tag="SCM56_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEG1110540.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5229..7274
                     /gene="parE"
                     /locus_tag="SCM56_001776"
     CDS             5229..7274
                     /gene="parE"
                     /locus_tag="SCM56_001776"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEG1110541.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7286..9736
                     /gene="parC"
                     /locus_tag="SCM56_001777"
     CDS             7286..9736
                     /gene="parC"
                     /locus_tag="SCM56_001777"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEG1110542.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10013..12244
                     /gene="pflB"
                     /locus_tag="SCM56_001778"
     CDS             10013..12244
                     /gene="pflB"
                     /locus_tag="SCM56_001778"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEG1110543.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12488..13249
                     /gene="pflA"
                     /locus_tag="SCM56_001779"
     CDS             12488..13249
                     /gene="pflA"
                     /locus_tag="SCM56_001779"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEG1110544.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13334..14260
                     /locus_tag="SCM56_001780"
     CDS             13334..14260
                     /locus_tag="SCM56_001780"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEG1110545.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14442..15335
                     /locus_tag="SCM56_001781"
     CDS             14442..15335
                     /locus_tag="SCM56_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEG1110546.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15469..15594
                     /locus_tag="SCM56_001782"
     CDS             15469..15594
                     /locus_tag="SCM56_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1110547.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15728..16360)
                     /locus_tag="SCM56_001783"
     CDS             complement(15728..16360)
                     /locus_tag="SCM56_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110548.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(16508..17050)
                     /locus_tag="SCM56_001784"
     CDS             complement(16508..17050)
                     /locus_tag="SCM56_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HEG1110549.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            17185..17574
                     /locus_tag="SCM56_001785"
     CDS             17185..17574
                     /locus_tag="SCM56_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110550.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            17578..18342
                     /locus_tag="SCM56_001786"
     CDS             17578..18342
                     /locus_tag="SCM56_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEG1110551.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            18471..19418
                     /locus_tag="SCM56_001787"
     CDS             18471..19418
                     /locus_tag="SCM56_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1110552.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            19548..20288
                     /locus_tag="SCM56_001788"
     CDS             19548..20288
                     /locus_tag="SCM56_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110553.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         7193 a         3481 c         4316 g         5495 t
ORIGIN      
        1 aacatggtcg aatcaccatt tcattcaacc actatctgtg ccgtcgaaaa agacgggaaa
       61 tttgctatgg ctggagatgg ccaagtaaca atgggtgaat ctgttgtcat gaaaggcact
      121 gcaaagaaag tacgacgtat ctacaatgga gaagttgttg taggtttcgc cggaagtgtt
      181 gcagatgcgt ttactttaga agaaaaattt gaaggcaaac taaacgaata taacgggaat
      241 ctacaacgcg cagctgtcga attagctcaa gaatggcgga cacagcaatc tatgcaaaaa
      301 cttgaagcga tgctgatcgt aatgaatgat aaagaaatgc ttcttgtttc aggtacagga
      361 gaagttatca caccagatga tggtatctta gctatcggat caggagggaa ttttgcgttg
      421 gctgcagccc gagcaatgaa aaactatgct caaaacgaca tgagtgcgaa agaaattgca
      481 gaaaatgcgt taaatattgc cgcagatatc tgtatcttta ctaatcataa tattatcgta
      541 gaagaactgt aagaggtgag tagatatgta tgaattaaaa caaacaccaa gacaaatcgt
      601 aagtgaatta gatgaatata tcgtaggaca gcacgcagca aaaaaatctg ttgccgttgc
      661 tttgcgtaac cgctatcgcc gtttgcagtt agatgaaaaa atgcaacaag atattacacc
      721 aaaaaatatg ctgatgatcg gacctacagg ggtcggtaaa acagaaatcg cacgacgttt
      781 ggcaaaaatc gtcaatgcac catttgtcaa agtcgaagca acaaaattta ctgaagtcgg
      841 ttatgtcgga agagatgtag aatcaatggt tcgggattta gtagaaaatg cgatccaaat
      901 cgtagaaaaa gagcagtaca gccgtgtata cacaagtgct aaaaaaaatg cagaaaaacg
      961 tttggtaaaa atcttggtac ctggtatcaa aaaagaacag aaacaatcca ctaatcaatt
     1021 tgaacaaatg atgaatcttt ttaatgctag tcagcaaaat accgctgaac cgcaagaaga
     1081 agtgacagaa gatattcgaa tcaatcgccg tacagtcttg gaacaactac aaaagggttt
     1141 actggatagt cgtgaagtaa ccattgaagt tgaagaccaa aagaaacctg caccaatgat
     1201 gaacaatgga ttggagcaaa tggggatcga cctaaatgaa actctaggtg ctttgacacc
     1261 gaagaaaaaa gtggaacgga cggtaacagt caaagaagct ttagagttat tgatcaagga
     1321 agaatcagca aaattagtca aggatgctga tatccatagt gaggcaatca aattagcgga
     1381 agaaagtgga attattttta tcgatgaaat cgataagatc acttctaaat cacagcaatc
     1441 tggagaagta tccagagaag gcgtccaaag agacatattg ccaattgtcg aaggttcaca
     1501 agtaaacaca aaatacggga tcatccaaac agatcacgtc ctattcattg cttcaggagc
     1561 tttccatttg tctaaaccaa gtgatctaat tccagaactt caaggacgct tcccgatacg
     1621 ggtagaatta gatgatttga cagctgaaga ttttgtgaag attctgaccg aaccaaataa
     1681 tgctttagta aaacaatacg tcgcgttgat cggtacagaa aatgtgtcag ttacgtttac
     1741 aaaagaagca attgaacgga tcgctcaaat cgctttcgat gtcaaccgtg acacagataa
     1801 tatcggcgca cgcagactgc ataccatttt ggaaaaacta ctagaagatc tattatttga
     1861 agcaccagat atgcaaatgg gagaaataac gatcacagaa gcatatgtgg atgacaaact
     1921 tggctcaatt gcggcaaatg aagatttaag tcgctatatt ttataaaaaa tcggaggacg
     1981 acaatgacag acttgctaac gaaaacccgc aagataaaca cacttttgca gcaaaagaat
     2041 acttttgaca tgcaagcaga tttgccttac gataaaatgg ctgtcacact aggtggtatt
     2101 cttgacagca acacctatat cataggaagt gacggagtat tactaggtta taatgaacgt
     2161 cacgatgtga acaatgaacg aatcaaaaca atgtttgttg agaaacaatt tcctgaatct
     2221 tatactgata cagtggcaca gttgatgaaa actgaagcta atatcccaat tactagtgat
     2281 attactgcat ttccggtaga gtcaagagag aaatatccat ttggtctgac gactgtcgtt
     2341 ccgatctttg gagctggcga acgtttgggt acgattatct tatctcgtat ggaacaatcg
     2401 tttgcagatg acgatctagt cttagcagaa tatggagcta cggttgtcgg tatgcagatc
     2461 ctttatcaga agtctcgaag catcgaagca gatgtacgga gtgcaacggc tgtccaaatg
     2521 gcaatcaaca cattatctta tagcgaattg aaggctgtac aagctatctt tgaagcattg
     2581 gatggagatg aaggtcgtct gactgcttct aatattgccg atagtatcgg catcactcgt
     2641 tctgtgatcg tcaatgcttt acgaaaattg gaatcagctg gaatcattga atctcgttcg
     2701 ttaggcatga agggaactta tttaaaagta ttgaattccc gttttaaaga cgagttagca
     2761 aaacacagtt attagaagta aggggactgg ggcagataga aatgtttgtt ccagccttcc
     2821 cattttaaaa agtaagtcga agttggagga atggcaatga ctgttattat tcaaaatgac
     2881 cagctgatcg ccgaaatcgc tgaacacggt gcggaattga tcagtctgaa atcaaaagaa
     2941 catggtttag agtatatctg gcaaggtgat ccagcgtatt ggggcagaca tgcaccggtt
     3001 ttattccctt ttgttggacg tttaaaagat gatcagtatg tttatcaagg gaaaacatat
     3061 caaatggggc aacacggatt tgctcgggat atggattttg aagtcattga acatggacaa
     3121 gaaatggcta gtttcatctt aaggagtacg ccggagacat tagaaaagta tccatttgat
     3181 tttgaattag tgatcagcta tgaattagga ggagacggca tcacggttca ttatcaagtg
     3241 gcaaataccg gtgaaaaaga aatgttcttt tctatcggtg gacatcctgc tttcaatgta
     3301 cctcttgaag aaggattgtc atttgaagat ttctatctgg cattttctcc attgaaatca
     3361 agattacgcc taccattgaa aggcccattc atcgatatgg atcaaaagac gttaggtcag
     3421 acaaatacga atctggcttt gaatcgtgaa ctttttgagc aagatgcctt ggtttttgaa
     3481 acaaggggtc taaatgcgta cgccattcga agtgaaaaat cttcccatag cgtaacttta
     3541 agctataaag atatgcctta tgtagggatc tggtctcctt atccaaaaga agcgcctttc
     3601 gtttgtatcg aaccgtggtg cggaatcgct gacacggtga acagtagtgg aaacttgatg
     3661 gaaaaagtag ggatcaatca actaggtgcc catgaaatct tcaagacaaa atattccatc
     3721 acagtgaagt aagaggttgt gaccgagaag ttagcaggga acaccgttta ccaaggtgtg
     3781 taaaaaagct atcctgtaag taataaggga gtgtgacatc gatttgtcac actcccgttt
     3841 tatctctttg gaatgaaaaa gtatcaggat tttttcttgc tgctattcaa accaaacggg
     3901 attcgacttt cagtatggtt tctgatacgc ttgatattgt ctttatgccg ataaaagata
     3961 aaggaagaaa ctaaaaaggc aatgatcgtc agtaaccaat tgggcttagc caaaatcaca
     4021 ggagctacat aaggaaggac gatcgtagat aaagtgatca atactgcact gatcatactg
     4081 ctcaaactca ccatacttgt acaaaataaa ctaacgataa agatgataca agaataaaca
     4141 aaaaatacag gattaaatcc taaaagcatt cctgcacttg tcgcaacagc ttttcctcct
     4201 ttgaacccag caaaaatcgg aaatgtatgg ccaagaatgg cgcatacccc aaaccaaaga
     4261 gggttaatcc cagaaaggtg gaagatcatc ggcaaacaag tagccaatgt accttttaaa
     4321 atatccataa gcaggacaac aatcccggct gttttgccta atacacgaaa tgtattggtc
     4381 gttccagtat taccgctccc gtgttgtcgg atatcttttt tgaaaaacaa tttgccaatc
     4441 cagacaccgg aaggaataga acctaaaaga tatgctaaaa taagtaataa gatcaatttc
     4501 attttttacc ctctttcaag ttgaaaacta tcctttattt tagcatgaat aggtaacagg
     4561 aacaattgaa aaaatgaaag atacaagtat aatagatgga ataaaaagaa aagcaagggg
     4621 tggcagaaat gtctgtaaaa aatccaacac aagaaacgat tttcaactat ttggaagaag
     4681 caaaaaatat tgccgtcgta ggcttgagca ataaaccaga acgaacaagt tacatggttg
     4741 cacaggtatt gcaagacaat ggatataaaa tcataccagt aaatccatta ttggctggtg
     4801 aagaaattct aggcgagaaa gtctatgcga atttgacaga aatcgatgat ccaatcgata
     4861 tcgtagatgt atttagacgg agtgaatttt tacctgaaat agcaggtgat tttttgaaaa
     4921 ctgatgctaa agtgttctgg gcacaattag ggattgaaaa caaagaagca gctgagatgc
     4981 taagaaaagc aggacgtaca gatatcgtaa tggatcgttg tattaagatt gaacttggta
     5041 agttgaaaga aaaccaaaaa taaaaatgac cacaaagaag agatgatcga tagaggggaa
     5101 tgtcgaaaaa gcggttagga gataattaaa attgatttct cgtctaccct cttttttttc
     5161 acgacctttt ctagtatgat aatcgtggtg cggttttgcc gcaaagtatt attgaaggag
     5221 cgtttgcctt ggcaaaaaaa gtaaacaacg aatacaatga ctcgtccatc caagtattgg
     5281 aaggattaga ggcagttaga aaaagaccag ggatgtacat tggatccact gacagccgag
     5341 gcttgcatca tttagtctat gaaatcgtcg ataatgcagt tgacgaagca ttatcaggtt
     5401 atggcagtga gattgatgtg accatccatg aagacaatag tataactgtt gcagatagcg
     5461 gacgtgggat gcctgtagga atgcatgctt ccggaatacc aacagttgaa gtgattttta
     5521 ctgtgctgca tgctggcgga aagtttggtc aaggagggta caaaacttct ggtggactgc
     5581 acggtgtagg agccagtgtg gtaaatgcct tatcgaaatg gctaattgta acgatcgtta
     5641 gagatggcgt agagtatcag cagaaattta aaaacggcgg taaacctgac ggtactttga
     5701 aaaagatagg aaaaacaaaa aaagcaaacg ggacgactgt ccatttttta ccagatgata
     5761 caatattcag tacaacgaaa ttttcatatg aaattttagc cgaacggtta agagaatctg
     5821 cctttcttct aaaaggcgtt aaaatctctc tttctgatct tcgtggagaa gaaccagtca
     5881 aggaaatttt tcattacgaa gaaggaatca aagaatttgt tgattatctg aatgaagaaa
     5941 aagatacatt gacaccagtt gtttattttt caggagaaaa agaaggtatc gaagtagagg
     6001 tcgcttatca atacaatgac ggttattcag aaaatgtttt atcttttgtc aataacgttc
     6061 ggacaaaaga tgggggaacc catgaagctg gtatgaaagc ggcgatgacg aaatcttata
     6121 atgaatatgc gagaaaagtt ggtttgctga aagaacggga caagaatctg gaaggtagtg
     6181 atttcagaga agggttagct gctgtacttt ctatccgtgt acctgaaaat cttctgcaat
     6241 ttgaagggca aacaaaagaa aaacttggta cgccagttgc tcgtaccgta gtagataacg
     6301 tcatcagtga acaaatgggc ttttatttgc aggaaaatag cgaaatgagc cagatgttgg
     6361 tccgtaaagc aatcaaagcc agagaagcga gagaagcagc acgtaaagcc agagaagaaa
     6421 gccgaaatgg gaagaagcgt aaaaaaggcg aatctcttct ttcaggaaaa ttgacacccg
     6481 cacaatcccg aaaccctaag aaaaatgaat tgtatttagt cgaaggggat tctgctggcg
     6541 gatctgctaa acaaggaaga gacagaaagt tccaagcaat tctaccttta cgaggaaaag
     6601 tcatcaatac cgaaaaagct aaaatgcaag atatcttgaa aaatgaagaa atcaatacga
     6661 tgatctatac gattggtgca ggagtcggtc ctgagttttc tatcgaagac tgtaattacg
     6721 acaaagtaat catcatgacc gatgcggata cagacggcgc ccatattcaa gtattactgc
     6781 ttactttctt ttatcgttat atgaaaccgc tgattgaagc tgggaaagta tatatcgcgt
     6841 taccgccttt atataaagta tccaaaggac aaggaaagaa gcaagtcatt gaatatgcgt
     6901 ggacagatga cgaattggca gcaatgatta aaaaagtcgg caaaggatat atgctgcagc
     6961 gatataaagg acttggagag atgaatgcgg aacaattgtg ggaaacaacg atggatccga
     7021 cttcccgtac gttgatccgc gtccgtatcg atgatgctgc gcaagctgaa cgtcgagtaa
     7081 ccacactgat gggtgacaaa gtagaacctc gaagaaaatg gatcgaaaat cacgtgcaat
     7141 ttacgctcga agaagatggc agtatcttag ataaaaaaga ggatactgaa atctctcctt
     7201 cagtttctaa tgatttatta gatgaagaac gagcagataa aaatgaaaac aatcagttat
     7261 ttgaagtaga ataggaggtc acctgatgga aaatcgacaa gaagtacaag aattaacttt
     7321 agaagaagtg atgggtgacc gcttcggacg ttattccaaa tatattatcc aagaacgtgc
     7381 actgcctgat atccgtgatg gattgaagcc agtgcaacgc cggatattat ttgccatgaa
     7441 caaagatggc aatacgtatg acaaaggatt ccgtaaatca gcaaaatctg tcggaaatat
     7501 catggggaat tatcatcctc atggtgacat cagtatttat gaagcgatgg tccgcatgag
     7561 ccaagactgg aagttgcggg aagtactgat cgaaatgcac gggaataacg ggagtatgga
     7621 cggtgatcca cctgctgcga tgcgttatac tgaagccaga ttatccaagt taagtggaga
     7681 gatgctagca gatatcgaga aagaaacagt cgatctcgtg tggaattttg atgatacaga
     7741 aaaagaacca acagtccttc cagctcgata cccaaacctt ctcgtcaatg gatcgacagg
     7801 tatatctgct ggatatgcaa cagaaatccc gactcataac ttagctgaag tgatcgatgg
     7861 cacaatctat atgatcgatc atccacaagc gagccttgaa aaattgatgg aatatattcc
     7921 tggacctgac tttccaacag gagggatctt gcaaggaaaa gatgaaatca aaaaagctta
     7981 tgaaacagga cgaggaaaag ttattcttcg ttcgaaaaca aagatagagg caatgaaggg
     8041 taataaacag caaatcgtga tcacagaaat cccatatgaa gtaaataaag cgaccttggt
     8101 gaaaaaaatg gatgagatcc gtttgaataa gaaaatcgat ggaatagctg aagtacgaga
     8161 tgaaagtgat agaacaggtc tgcaaattgt cgtcgaattg aaaaaagacg ccaatgctca
     8221 aggaattttg aattatttgt tcaaaaacac tgagctacag atcaattaca atttcaatat
     8281 ggtcgctatc gaccatatga ctccgcatca agttggactg aaagacattt taagaagtta
     8341 tatcgagcat cgaaagcaag tcataacaaa acgtagtcaa ttcgatttag ccaaagctca
     8401 aaaaagacag catatcgtag aaggattgat gaaagcttta tcgattctgg atgaagtaat
     8461 cgcaacgatt cgtgaaagta aagacaaaaa agacgccaaa aagaatttag tcgacgtctt
     8521 tcaatttaca gaagaacaag cagaagcaat tgtcacattg caattatacc gtttgacgaa
     8581 cacggatatc acagagcttc aaaaggaatc cgaaagcttg attgcgcaaa tcacagagtt
     8641 gaataaaatc ctttcaaatg acaaagagct tttctctgtt atgaaaaaag aattgcgaga
     8701 agtgaaaaaa aattattctt ccgctcgtct aacgacaatc gaagacgaaa tcgaagaaat
     8761 caaaattgat acgcaagtct tagtggcaca agaagacgta attgtcagtg tgacgcgaga
     8821 aggctatgtg aagcgaacaa gccttcgttc ttatagtgct tccaaaccag aagaaatcgg
     8881 gatgcgagaa ggcgactact tgctatactc aggtgaattg agtacattgg accatgttct
     8941 tctgatcaca aataaagcaa atgtgatcta ccgccctgtc catgaactac ccgatttgaa
     9001 atggaaagat gctggtgagc acatttctca aacaatctcc aatctgtctg tagacgaatc
     9061 gattttagct gttttcccat atcaaaaaat tcaagcagag aaaacatttg tattcatcag
     9121 caagaatgga ttgatcaagc aaacacgaat gacagagttc gaaccatggc gcacctataa
     9181 gagccgtccg ctcagctgta tgaagctaaa ggcggaagaa gatgaactga ttgctgtgta
     9241 tctgaaagaa gatcagccag gcttagatgt tttcttagta acgcatcaag gaatgggttt
     9301 gcgctatcct ttaacagatg ttcccgttgt tggagcaaag gcagctggag tgaagtctat
     9361 gaatttgaaa gatggagata ggattatcaa cgggctgctt gtgttagcag aaggtgatac
     9421 gccgatcgta atcgttactc agcgaggagc tgttaaacgc atgcttgctc aagaaatcag
     9481 ccaaacttca cgtgcgaagc ggggagtgac ggtcatgcgt gagctgaaaa aaaatccgca
     9541 ccgtattatc tatatgtcgg aaggaagatc aaaagaaata accttgatga atcaaaaagg
     9601 gcagcagtta acgattgacc caactgattt ccctataggt gatcgaacat cgaatggatc
     9661 atttgcgatg gatgagaaaa aaggtggaga agtcatcgaa gttatcgatg ctcctgagat
     9721 tttcatcgag tcgtaactgt tgtagtattt taatgaagag acgccatctg tattaataca
     9781 ggtggcgtct ctttttcatt catttttaat aaatccttat acgtttgcaa aaaaagatga
     9841 aagtgttcac aatgcttaaa tgttggtatg tcaacgttga tttaaatata tgtaaataaa
     9901 acataaaaat tgttaaataa aaaacaagaa aacccttgca aagatagggt gctgtgttat
     9961 actaatcacg taaaaactgt tacataaaac agaaatttag gaggtccata caatggagca
    10021 atggaatgga tttaaaggta ccaaatggaa agaaggcgta gacactagag actttattca
    10081 aaataactac actgaatatc gcggtgatga cagctttttg gaaggcccaa cagaagcaac
    10141 agaaaaatta tgggatgaat tgcaaaagtt aaatgatatc caattcgagc gtaacggtgt
    10201 ctatgatatg gacacagaaa tcgtatcaac tattacttca cacgatgcag gctatttgga
    10261 taaagatttg gaaaaaatcg tcggtctaca gacagacaaa cctttgaaac aagcattcat
    10321 gccgttcggc ggaatcaata tggctaacaa tgcgctaaaa agcaatggct atacaccaaa
    10381 cgatgaattg acacatattt tcactgactg gcgcaaaaca cataatcaag gtgtatttga
    10441 cgcttatact gctgaaatgc gtgctgctcg taaaaacaaa atcattacag ggcttccaga
    10501 cgcttacggt cgcggacgta tcatcggtga ctatcgtcgt gtggcattgt atggaatcga
    10561 ctatttgatg gaacaaaaga aaaaagatca tgatatgact ggttatcata caatgagtga
    10621 agaagtgatt cgtttacgtg aagaaatcac tgaacaatat cgtgcattag cgcaaatgaa
    10681 agaaatggct gcgaaatatg gctatgatat ttctaaacca gctgcaaatg caaaagaagc
    10741 tgtccaatgg ctatacttcg gatatttagc agcaatcaaa tcacaaaatg gtgctgcgat
    10801 gtcaatcgga cgtatctcag cattccttga tatctatatc caacgtgatt tggataatgg
    10861 agtaatcaca gaaaaagaag cacaagaatt gatcgaccac ttagtaatga aattacgtat
    10921 ggtcaaattc gcgcgtacac ctgaatataa ccaattattc tcaggattcc ctatctgggc
    10981 tactttatcc attgcaggta tgggattaga tggtcgttca ttagttacaa agaatgattt
    11041 ccgtctgctt catacattaa caaatatggg accttcacca gaaccaaact tgactgtttt
    11101 gtattctgaa caccttccag aaggattcag aacatatgct agcagaatcg ctatcgaaag
    11161 ttcttcgatt caattcgaaa atgataatca gcttcgtgaa caatggggtt ctgatgattg
    11221 tgcgattgct tgctgtgtat caggaacagt tgtcggaaaa gacatgcaat tctttggcgc
    11281 tcgtgctaac ttggctaaaa cactattata tgccatcaat ggcggagtcg atgaagtgac
    11341 tggcgcccaa gttggaccag aatatcgtcc aatcacatca gaaatactag actttgaaga
    11401 agtgaaacat aacttcatga atatgatgga ttggttagca gaactatatg tcaatacgtt
    11461 gaacatcatt cattatatgc atgataaata ttcttatgaa gcagctcagc tggctttgat
    11521 ggattctgac ttgcaacgca catttgctac aggtattgct gggatctctc atgcggcaga
    11581 cagcttgtca gcgatcaaat atgcaaaagt aaaagcaatc cgtaatgaaa aaggaatagc
    11641 agttgatttt gaagtggatg gcgacttccc taaatacgga aatgatgatg accgtgcaga
    11701 tgaattagtt tcatggatct tggaatactt catgggacaa atcaaacgtc ataaaactta
    11761 ccgcaactca aaaccaacaa cttcattatt gacgattact tcaaatgttg tttacggtaa
    11821 aaatacagga aatactcctg atggccgtga agctggaaaa ccacttgcgc caggagcaaa
    11881 tccaagttac aatgcggaac aaagtgggtt attagctagc ctgaactcaa cagctaaaat
    11941 gaattacggc tgtgcattgg atggaatttc aaatacccaa acaatcaatc cttcaggtct
    12001 tggaaaagaa caagatacaa gaatcgaaaa cttgcgtaat gtcttagatg gatacttctc
    12061 aaaaggagct tatcatttga atgtcaatgt attcagtaat gatttattgc gtgatgcaat
    12121 ggaaaatcca atgaaatatc cgaacttgac gatccgggta tctggttatg cagttaaatt
    12181 ccgtgacttg acaagagaac aacaattaga tgtcttgatg agaacttcac acgacagaat
    12241 gtaaaaagtt cgtgaaaaaa gtttttcgac ttagcgtcga aaggacgttg aatagccatc
    12301 gcatcaaaat cgaataatca gtatgcttat ttgaaagaaa cgatttacag gggcaggact
    12361 tgtatgttcc gtccctgtaa attttacaca taacgaagcg gcaattgcga aaaattttgt
    12421 tcagaaagct tcgcttcgac agctaacttg tacttaaaat gaaatcaaag aaaagatgtg
    12481 aacttaaatg gaagaaaaaa caatcggcta tgttcattct atcgaaactt tcggttcagt
    12541 agacggacct ggactgcgct ttgtcgtgtt tatgcaagga tgccgcatgc gttgccaatt
    12601 ttgtcacaat ccagatactt ggaatattgg cggagggaaa gaatacacag cagatgaact
    12661 tttagataaa gcagaacgct tcagacctta ctggggagat aaaggtggta ttactgttag
    12721 tggtggagaa cctctattgc agatcgattt tttgatcgaa ctatttaaaa aagcaaaaga
    12781 gcggaagatg catactacat tggatacatg cggaaaacct tttacgtatg aagaaccttt
    12841 cttttcacgt tttcaagaat tgatgaagta tacagattta ctgttatttg acatcaaaca
    12901 catcgataat gaagagcata aaaaactaac gcattgggac aatgaaaata ttttagaaat
    12961 ggctcaatat ttgtcaaaaa tcaacaaacc ggtttggatt cgccatgtac tagttcctga
    13021 gcgaagtgat tatgatgaat atttgatccg actagacaac tttattcaaa ccttgtcaaa
    13081 tgtagacaga gtagagattc ttccttatca tacgatggga aaatacaagt gggaaacact
    13141 tggtttgaaa tatccattag aagggatcga accacctaca aaagaacgtg tcgaaaatgc
    13201 taaacgattg cttcatacag aagagtatac aaaatattta acgcgatagg tcgcatcgga
    13261 aagaagataa gtattgcttc tttccgtttt tttgctatta ttaaatagga gaaatcacaa
    13321 aggagcgaat gacatgtcaa aaattttagt ttttggtcac caaaatccag atacagatgc
    13381 aatcggggca gccattgcgt tttcacactt gcaaaatgaa ttaggtaaag aaacagaagc
    13441 agttgctcta ggtacaccta atgaagaaac acaatacgca ttagaccact ttcagctatc
    13501 tgcaccacga gtagtagagt cagctaaagc agaaaatgca gatgtcatgc ttgtggatca
    13561 taatgaattc caacaaagta tttcagatat cagcgaagta aatattttag ctgtagtaga
    13621 ccaccatcgt attgcaaact ttgaaacagc taatccattg tactatcgtg cagaaccagt
    13681 tggatgtaca agtacgatta ttttaaaact atataaagaa aaccaagtaa caattcctaa
    13741 agaaatcgca ggaatcatgc tctcagctat catttcagat acattgttgt tcaaatcacc
    13801 aacatgtacg cctgaagatg aagcagctgc aaaagaactt gcagcaatcg ctgaagtcga
    13861 tctaaacact tatggacttg aaatgttgaa agcaggaaca aatctaagtg ataaatcagc
    13921 tgcggttctt cttgatttgg atgcaaaaag tttcccaatg ggcagttcca acgttcgtat
    13981 cgctcaagtc aatacagtgg accttaatga agtgatggat cgacaagcag agctagaagc
    14041 agcaatggaa gatgaaatga aagcaaatgg ctacgatctg ttcgtattag ttgtcacaaa
    14101 catcttagac agtgattcag aattacttgt tgctggtaaa ggaaaagaaa aagtagaaga
    14161 agctttccaa acaactttga ccaacaaccg tgctttgctt aaaggtgtcg tttcacgtaa
    14221 aaaacaagtc gtacctcaat tgacggctgt atttagctaa tcaaatggta gtatgaagca
    14281 ttcaaaatga atggataagc gattgatata aaaagaaaca tagaacatac taaaggccga
    14341 agaaatggag gagaataaca gtcaattatg actcttgttc ttcctccttt ttacgtaaaa
    14401 gccttttaag taaaataaaa cggatagaaa agagaacata tatgaactat tattcaaaaa
    14461 ataaggaaaa cttttatcaa aaactgaccg atgatccgct tttttcttca ttgaccgatt
    14521 atctatatga acatcgagaa caagaaacga ttctaagaga attgaaaaaa gaattttcgc
    14581 aaaataaatt ttcccatttt cttgatttat tgatcgatgc aggattaatc aagagggaag
    14641 aacgaagata tcatttgaac tttccaatat ttgatcctaa agattacttg caacaagcca
    14701 cttcggctgc tgaaaccata gctgaacaat tgaagagact ctctgttgat gaacaaaaat
    14761 tagcgatggg agaagttatt tgggcgtatt gtttcgaaga tgaacggaaa gaagcgtatt
    14821 tttatggagt aagaaacagc cgagagacag aattgctgag gacaactgca ggaaatgaga
    14881 agtaccgttt tatcacactt tcctctatag aacacttccc gcttactttg gctaattact
    14941 tttttgtaca gaagaatcag cttcctgtaa cgaaggcatt caaagaatta gccgaactta
    15001 ttggagacgt aaatgaatcg tacttttttg atcaaataga agttatcgtt gacaggatac
    15061 gtaagaataa atataaaaat cgccggccta gtattttcca tcagtctcta cttgtgacga
    15121 atactataaa ggaagaagaa tcattcacat tagaattacc tattgtcgaa aaaaacaatt
    15181 ttgagattga attaccaaca ttggatcctt cattgacaat ggaggaaatg gcctttctta
    15241 aacgacaaat cttttctgag ctgagcaaga aatttatacc gcatgcattt tcttatatca
    15301 aagaatatgg aacagtttta gtgagtaaaa cctaagagag cagtaagcaa aaattatact
    15361 gactcttgca ggaggagcct ttctcttcct ttatggttaa aagaattatt tgagtatttt
    15421 gcctatctac cacctattag attcactaca tggtctggaa acaaaggaat gcaaaaaaat
    15481 ggtgagaaat gtggaatgac aaaggaagta gtgataagaa aagttagatt tttaaataat
    15541 caatattatg attcagtcaa gtatggcatt ttatgggaag aactagctga ttagattagg
    15601 aagtccattt gataatccag aatcactaag aaacgaatgg cgagtcatta ataatttatc
    15661 caatcttggg ttattctttt agattgaagt gtttaaaact aaaaaagcat ttatgaagtc
    15721 gtaatagtca ggcttcaaaa tgctttttat tttgtttgtt ttttctttgc cacataaata
    15781 cacctagact gacgatgaag agcatatcca acagatttcc tgcgacttgg atcgtcgaag
    15841 ggttggtcag tggcattaaa gcaaggacaa aaaaggaaaa caagctgtaa ataaaacagg
    15901 tgacaggaac taatcgaaag aagagcggac atttcaattg gctaaaaatg agtgaaaaaa
    15961 taaaataagt aaaaatggat aaaatcacga ctaaaccatt catgataaag aagaaaagcg
    16021 tattgtgatt aaacgaataa aaaagaattg ctaaaaaaga aaacgtttta aatgcaatca
    16081 atacgcttga agcttcgttt aacgtaccag gatgtcgatg aaaaaaatag gcaccgaaat
    16141 aaacagaaaa cagtaagccg caaagagaag acaaaatcaa aaaaaacgga ttttcttcgg
    16201 cttctgtgat atcagaatga tccaaaagca accagcctaa tgcactagaa ggcgcatgtg
    16261 aggtcttaag gtatgaataa catagaaaaa ataagatcgt caaagttgca aaaaaagagc
    16321 ctaaatgtaa aaaattagtg gttctacgtt gaatttccat aatatctcca aattaaattg
    16381 ataagtatat aaagtataat aacaaattga tgagtatatg tcgatgtttt ttaatcaaaa
    16441 taataaataa aaactgtcta acatttaaaa tttaatttag atattagaca gtttttaagt
    16501 ttgtgagtta ttttccttgt cgttttctca cttttttagt ccagaaacca cctgaaatat
    16561 attttggctc ataagaaatg ataaaagcac gctcttcgat ttctttgatc aggcgataca
    16621 gttcacgttc gctttttcta ggagaaagta tttcgagaac tagccgctca ccttccagcc
    16681 ccattgcaga agtctgtgtc acaccataac cgtgttctct cagtataccc ggcaaacttt
    16741 tatcttctgc gctatttgct ggcaaaatcg ttgtgaccat gatgtatcct aatgccaatt
    16801 tgtcttcaat caaaattccg acactgatac ctactgcgta acctaacgca tagacaaata
    16861 aattcagtgg attatctaaa cgatctaaaa caagggataa gccgacgata taaatcgtga
    16921 tctcgaccat actgacaagt ggagcaatca gtcgatagcc tttcattgtc aacatgaaac
    16981 gtaacgtgtt cagagtgata taagcaaaat taatgaggaa gatcatcccc aacatttttg
    17041 gatcaatcat ttgtaaaact cctttcattt atataacaac aatatcaaaa aaaatcgtaa
    17101 gtgaagaaaa attgaaactt tatacataca aaaaaatgat aaaatgatta taatagaatt
    17161 ataaagatag gttaggtgat taaaatgaaa acggtcaatc gtgtgttttg tgccagccac
    17221 gtgctaagtc tccttctcag tatatactta ggtgtctatt cgattcaaca gccaattgag
    17281 gtagagaaca ttgtttttca gatgtcgctg acagatggtt taattttaag cgtttttttc
    17341 tttttgatac tgtttattgg taatattttc ggtagcgttg ccagtttttt gaatttttct
    17401 atttatccac tcctttcgct agcattagga gtggtgggac tttgttctct gtgcttattt
    17461 cccgagccat tcagtcctgc atttatatta tttggtacac tgaacctgtt ccagacaact
    17521 gttggagcat ggttgctatg gcgatcagga aatttaatga aaataggtga gtaacaattg
    17581 aatccaatta tattgaaacg ttctgtccat catattccag tgttagaagt tgttctgaaa
    17641 gaattaagag aggaacgtat tcccgttgtc atctattatc acggatggca gacaagtaag
    17701 gagttagttt tgacgcaagg aagaaaaatt gcgcagcagg gcattcgtgt gatcctgcca
    17761 gatgcgatga atcatgggga gcgcaaacag ccggtttctt ctattccatc ctatacattt
    17821 tgggatagta tctacggaaa tctgtttgaa tttgacacac ttatcaagca tttagaaaaa
    17881 agaggtcttc tacatgatca gcttgcagtt ggcggggttt cgatgggggg aatgacaaca
    17941 tgcgccttgt tggctaaaca tcctcatata gtagcgggcg tgtgtttgat gggttcccct
    18001 gcacctttgg aatatggaaa agaaatcgta aggcgcgcat cggaatataa ttatgtcgta
    18061 ccaaaagact attttgattt gatttcatgg gttaagagct atgatttgtc attgcagccg
    18121 gaaaagttag ctggacgtcc tctgttcttt tggcatggac aagctgacga aaaaatccct
    18181 tatatacaaa cggaacgatt tgtagaaaaa aatcggcata agtcttacgg aaaagaaatt
    18241 gtttttcgtt cctcaaaaac acagcgacat atggtacaaa taccactaat ggaagaagct
    18301 tccacttttt tagcaaaaaa tttgataaaa aagtataaat aattgaaacg gaaaatcact
    18361 catgacgata gaggattttc cgttttttat tttgggctat aataaactct gttaggaaaa
    18421 tttggtataa ttgatagtaa gaatgacaac atgttagaaa ggaagagagc atgcccactt
    18481 tagcagatgt tgcaaaacgt gccaatgttt caaaaatgac ggtctctcga gtcatcaatc
    18541 atccggaaca ggtgactgac gaattgaaag agttagtttt ctcagcaatg gctgaactgg
    18601 attaccgtcc aaatattgct gcaaaagcac ttgtcacgaa tcggtcgcag atcatcaaat
    18661 tatttatact ggaagaaatc gacacaactg agccctatta catgaatttg ctgatgggaa
    18721 tcgctaaacg gatcggtaaa gcgcattatt ctctccaatt agttacaaat gatgccttcg
    18781 acgtcggttc ttgtgatgga tacatcatca caggtgtcag aaaaaaagac ttcgattgga
    18841 tcgaccgttt ggaaaaacca gttgtcttgt ttggggaaaa ttctgaagga tatgattatg
    18901 tagacagcga taatgaatat ggtacagcaa aagcaacgca atatgcctta gaaagagatt
    18961 atcaaacgat tatttatatc ggtatcgaag tggacgagcc ttttgaaatt tcaagggaaa
    19021 ctggttattt atctgtgatg aaggaagttg atcttcctag taaaatcttg cggtttccaa
    19081 atcattcgac agaagtagaa agatatatga cagaaaactg ggcagatttt acagataaaa
    19141 cttgttttgt atgtagttca gatcggctag cactaggagc agtaagaggg attataaatg
    19201 ctggaggaca cttgcccgaa gaatttggtg tgattggatt tgatggggta tttttagacc
    19261 aaatcagttc acctaaattg acaacgatca agcaagatat tatacgtatg ggtgaagtat
    19321 gtggagaaat gttgttaaaa aaaatagaag aaaaaggggc gagtcaaggt tatcgtcgtt
    19381 tttttcctga actagttatc agagagacaa cgaattaagt cttattacta tatatatatt
    19441 tatgaaattc atcgctattt tcttaaaata atcagcgcat tgctactgaa aacaatgaat
    19501 ctgtgctatt attgagaaaa gttccatttt tgagaaggat ggtttgaatg aaagaaatca
    19561 gacgaaaaca tttccgtttt cctgcgtacg atgatgagtt aggcgtaaaa ttaaccaacg
    19621 acaagaagcg agtattattt gacggacaag aagatctttt gactgaacag aatcagtccc
    19681 cgtttgaaca gatgaaagaa tttcatttca cgaacacgaa caccgagaaa atccgtcgca
    19741 agcgtcgtgc aacaaatgga ttgaagtcag ctccgcgtaa ggaaaatttg gaaaagcata
    19801 aaggaaaatt accggattat accggccatt accaatcaga aatcacatcg acaggacgaa
    19861 aaaaactttt tggtgattcc tctcgttctg tcagacaaag tgaaacaagg acagagtcca
    19921 agcatactac acgaaacgca tctgttgaca cgaaggtgcg ggagcgttct tattttgttc
    19981 ctaaatatgt acccgcatcg attatcccag accctagaga aagcaaaatt tctgaggatc
    20041 atttaatcga atcgatgaaa aaaacggatg actcttatct gttgttcgat acagaaccga
    20101 cgccgtatca agaaaaaaga aatggtgagc cgtctgtacg aacattcaat cgaacaaaaa
    20161 cgttagagga aacaaagcag tcagaagaaa aaaaatcgaa aaaaagccat ggtatcttag
    20221 aacgctcatt acaaggattg atcgatgatc aaggaaactc tttgggagaa aatagctatt
    20281 ttaaataagt gatatacaaa tttatattcg gctacagttg gaaaggaaac gctgcggcgt
    20341 ttccttattt tatactctat ccgaaagttt atatcgtatc ctcaaatttt tttgagttta
    20401 agcgaatttt ttctcccaat aagtaagaaa ataatggcaa aataaaaaaa caaactattt
    20461 tcgcagatcg tttgcaaaaa tagtt
//
LOCUS       DARBDV010000054        20217 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000054 DARBDV010000000
VERSION     DARBDV010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.054"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..152
                     /locus_tag="SCM56_001789"
     CDS             <1..152
                     /locus_tag="SCM56_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1110554.1"
                     /translation="NFAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIY
                     MKKTL"
     gene            complement(230..763)
                     /locus_tag="SCM56_001790"
     CDS             complement(230..763)
                     /locus_tag="SCM56_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEG1110555.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(857..2035)
                     /gene="mutY"
                     /locus_tag="SCM56_001791"
     CDS             complement(857..2035)
                     /gene="mutY"
                     /locus_tag="SCM56_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEG1110556.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2028..2825)
                     /gene="recX"
                     /locus_tag="SCM56_001792"
     CDS             complement(2028..2825)
                     /gene="recX"
                     /locus_tag="SCM56_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEG1110557.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2932..4314
                     /gene="rlmD"
                     /locus_tag="SCM56_001793"
     CDS             2932..4314
                     /gene="rlmD"
                     /locus_tag="SCM56_001793"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEG1110558.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4488..5066)
                     /locus_tag="SCM56_001794"
     CDS             complement(4488..5066)
                     /locus_tag="SCM56_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HEG1110559.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5388..5858
                     /locus_tag="SCM56_001795"
     CDS             5388..5858
                     /locus_tag="SCM56_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HEG1110560.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(5966..7192)
                     /locus_tag="SCM56_001796"
     CDS             complement(5966..7192)
                     /locus_tag="SCM56_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEG1110561.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(7946..8146)
                     /locus_tag="SCM56_001797"
     CDS             complement(7946..8146)
                     /locus_tag="SCM56_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG1110562.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8280..8888)
                     /locus_tag="SCM56_001798"
     CDS             complement(8280..8888)
                     /locus_tag="SCM56_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEG1110563.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(8973..9443)
                     /locus_tag="SCM56_001799"
     CDS             complement(8973..9443)
                     /locus_tag="SCM56_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEG1110564.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9491..10405)
                     /locus_tag="SCM56_001800"
     CDS             complement(9491..10405)
                     /locus_tag="SCM56_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEG1110565.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10551..11354
                     /locus_tag="SCM56_001801"
     CDS             10551..11354
                     /locus_tag="SCM56_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEG1110566.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11532..12479
                     /locus_tag="SCM56_001802"
     CDS             11532..12479
                     /locus_tag="SCM56_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEG1110567.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12547..12840)
                     /locus_tag="SCM56_001803"
     CDS             complement(12547..12840)
                     /locus_tag="SCM56_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HEG1110568.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(12868..13533)
                     /locus_tag="SCM56_001804"
     CDS             complement(12868..13533)
                     /locus_tag="SCM56_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEG1110569.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(13673..14305)
                     /locus_tag="SCM56_001805"
     CDS             complement(13673..14305)
                     /locus_tag="SCM56_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1110570.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14312..15379)
                     /locus_tag="SCM56_001806"
     CDS             complement(14312..15379)
                     /locus_tag="SCM56_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEG1110571.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15376..16107)
                     /locus_tag="SCM56_001807"
     CDS             complement(15376..16107)
                     /locus_tag="SCM56_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEG1110572.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16276..16755)
                     /gene="greA"
                     /locus_tag="SCM56_001808"
     CDS             complement(16276..16755)
                     /gene="greA"
                     /locus_tag="SCM56_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEG1110573.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="SCM56_001809"
     CDS             complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="SCM56_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEG1110574.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            18270..19439
                     /locus_tag="SCM56_001810"
     CDS             18270..19439
                     /locus_tag="SCM56_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEG1110575.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(19706..>20217)
                     /locus_tag="SCM56_001811"
     CDS             complement(19706..>20217)
                     /locus_tag="SCM56_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110576.1"
                     /translation="LLILAISILNYSSVVNMVKEMNFQGTYSNEQMENKLEEFENFDY
                     VPIGYKKDQLLLKNHQVTEDGQVIYSSFSPNKDEITFTFKSSKGKTIVLPVFRYKGLT
                     VTVNSKNATVLKQGGPFVTVKSESGINKVTIRYSYTFFSKISLFISIITLIVLLFYCI
                     KLKIRTSHE"
BASE COUNT         6125 a         4026 c         3213 g         6853 t
ORIGIN      
        1 aaaactttgc taagaatcaa ggctataata cgattttatt aggggcacag tccacagctg
       61 aaacttttta cgaaaaacta ggatatacag cttacggcga tccttttgaa gatgctggca
      121 tgcctcatat ttatatgaaa aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca
      181 gcaagctttt gagaaaaatc tctcactggc ttactgttct tttttgtgtt tacttacgcg
      241 acaattgctt gtaacggtta taccaaatat caatatagcc ttccgagaac gggccatctc
      301 cattattgat ccagtcaacc agaatcttca cattttcttt taggatgaag tcaatatcgt
      361 tcgggtaatt catcattttg ctgtgcatct catattcatc gacatccaat agtcgtttct
      421 cgccgtcagg aaataccttg atatccaagt catagtcgat atatttcaag gcctcatcgt
      481 ccagcaaaaa aggtgaagct agattacaat aataggaaac ccccttttct ctgatcattg
      541 ctattacatt gaaccagtat tttttatgaa aataaacaat cgctggttcg cgcgttaccc
      601 atcggcgtcc atcagattca gtgactaatg tgtgatcatt cacgccgatc atagagtgtt
      661 cgcttgtttt taataccatg gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt
      721 aactttggat cgtcacaaac tctccttctt ttggaactcc catagcaacc cttccttaag
      781 cagcattttg tgctttgtgt ttgggtgttc attacaccga catcataatt attatatcaa
      841 taaaaatgag ctttgcctag aaaggattcc tattgtttcg atttttctct aactgatcta
      901 ccagtttcat ttggacctta ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact
      961 tcgaagtttt gttctcggta aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa
     1021 ggagaacatg ccatttcaga tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct
     1081 gccaaacaaa gagttcctgc ttttcaaatg ggagattttg ctcttcttct gctactaggt
     1141 catcaaacag atcgatttct ggctttgttt cccattcttt tttcaatcgc tcatactcct
     1201 cttgtgtcac ttccatcatt ggaaatgtcc acatattcgc cagcaatttt tggctatctc
     1261 tttcttcaaa ataatacgct ccagaatgat tttgaagagc tgcactgata taataaacgt
     1321 cttttggctt tgcttttttt gtttttacag gaaaactcgt ttggattcct cgtttgttag
     1381 ctaaacaaaa ggcttggatc ggacaagtct cacactttgg agaagttggt gtacagatag
     1441 ctgaacctag atccatcatt gcttggttga attcacctgg atgcttttca tcaatgattt
     1501 tccgcatcgc ttcatcaaag attttcctgc tagaagcttt tgcgatgtct gcttcaatgc
     1561 aaaacaatct gctgactact cgcatcacgt tgccgtcaac tgcaggttct ggaagtccaa
     1621 atgcgatact tgcaatcgcg cctgtcgtat atggtcctat tcccttcaat gagctaattt
     1681 cttcaggcgt ttgcggcatt tccccatcaa attctgacat gatctgttta gcagcagctt
     1741 ggatgttccg ggctcttgag taataaccaa ggccctccca tgcttttaaa agcttttctt
     1801 ctggagcatt tgctagctct tcaattgtag gaaaccattc catgaaacga taaaaataat
     1861 cgatcactgt gtctactctg gtttgctgaa gcatgatctc agagatccag atacgataag
     1921 gatcacgatt atagcgccaa ggcaggtttc ttttttcttg ttcataccat tgaatgaact
     1981 gatcttgaaa ttcttttgtt tctttatctg tccatttttc ccattcttta ttcactttcc
     2041 tcttcctttt ccgtaatgaa tcgctggatc atatccaaat caaatccttt ttgatataag
     2101 ctttgcttta cttttagatt tcttttttta ggttcaaacc ttaaatttct tcgccatagt
     2161 ttatctcctt gtttgactaa ataatcatat tctatttctt gatctgcttc ttgcggaaga
     2221 tcagctatcg cttgttggat cacttcttga gaaaagcctt tcgtcatcaa tgtttgttgg
     2281 atttttctca ataattcttt ttggcttttt ccgtgatttt ttccaaacgc tttttcggct
     2341 gtctgatgtg cattttgaac ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg
     2401 atttcaggtt tcaaaccttt ttgctgcatc tgctgcgcta atttctttgg tcctttgtca
     2461 cttaagcgca tattcgtgcg cacataactt tccccataga ccaaatcatc gacaaggtcc
     2521 aattctttta agcgggctac gattttattt cgatcttctt gaaggatttc cttttctttt
     2581 aagtatgttc ggatttcttt ttccgatcgc aactgataac ttaagtaatt cattgctagg
     2641 tgaaaaccga aatcagctcc gctactcttc ttgatctctt ggatagtttc ttcgtctaat
     2701 tcccgtcctt ttaataatcg atagcgtact aatacatctt cagatacttg taaagcttct
     2761 ccttcagata aatctacgcg atagaaaggg cctttgcctt tactgattct cgtaacggtt
     2821 aacataaaca tcctcctcga acatatattc tctatcatac cacagaaaat ccccgtaatc
     2881 aggtattata tgttagaata aaccacagaa aaaagaaaaa gggagatact tatggcagaa
     2941 acaattgtca aaactggaca aacggttcct ttgaaaatca agcgtttagg cataaatggt
     3001 gaaggaatcg gttatttcaa acggttgatc atatttgttc catatgcact accaaaagaa
     3061 gaagtactag tgaaaatcac gaaagcaact cctcgctatg cagaaggaga attaataaaa
     3121 gtcaaaaaaa caagcaaaga tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt
     3181 ggctgtcaat tacaacactt agcttatcaa gctcaattgg attttaaaaa ggatctgctt
     3241 ttgcaagcat tggaaaaatt caaaccagca ggctttcgat cttatcaact tttgccgacg
     3301 atcgggatgg atgagccttg gcattataga aacaaagcac aatttcagtt acgaaaaaat
     3361 aagcagactc aaaaaattga agctggttta tacgaagcaa actctcatca gttagtccct
     3421 ataacagact gcttggttca agaacctcga acacaaaaag taatgaatac tgttgttcaa
     3481 ctattgaata aatttgatgc acccatctat gatgaacgga caaacagtgg aatcttccgc
     3541 acaatcatgg ttagaatcgg tgttaaaact ggggagttac aagttgtttt tattactcgc
     3601 agtaaaaaat tcccgcaaaa aaacgcaatg atccgtgaga tcaatgaacg actgcccgaa
     3661 gttgtttcaa tcatgcaaaa tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa
     3721 acgatccact tatggggaaa agaagccatt gaagaacaaa tcaatgaagt gactttcgat
     3781 ctttctcctc gtgccttctt tcaactcaat cctcagcaga ccgaattgct ttatggcgaa
     3841 ggaatcaaag cattagatgt ccagcctaat gaaacgatcg tggatgctta ttgcggagtt
     3901 gggacgatcg gcttaagtgt agcaaaacaa gccaaagaag tacggggaat ggataccgtt
     3961 cctcaagcaa tcgctgatgc aaaaaaaaat gcggaaagat taggatatca caacactcga
     4021 tatgaagtcg ggacagcaga gcaagttctg cctaaatggt tgaatgaagg ttttcagcct
     4081 gatggtatca tcgtagatcc accacgtacg ggtttggata acgcattgat ccaatcatta
     4141 ttgaaatacc ctccaaaaaa acttgtttat atctcctgta atgtttctac acttgccaga
     4201 gatctggtta atttaagtaa aaagttcaag attgaatatt tacagtccgt cgacatgttt
     4261 cctcagactg cacgttgtga agttgtcgtg aagatgacga gactcgctga ctaaatgaaa
     4321 gaggttgtga aaaagtctgc cctgtatcaa gtaataagaa cagccaaaca aaaatagctc
     4381 ttcatgtttt atgaatactg tacttgtgac cgaagagtta acaggaggac actatttatt
     4441 taaaaaaagg gttgtgacat agtttttgtc acagcccttt ttgattatta ttggatattc
     4501 acgtctatca cttgataaaa tgcatttgtc gtatcgctga tattccatac tgctaaaatc
     4561 acattatatc cttttcgatc ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga
     4621 acagttgctt tgtcgtctat ttttgtgatc aattcaaaat cactaaattt caaaggttga
     4681 ttaggattcc aagttggttt ggtaatgtag taatcccaag tacttgtccg atgttgtgct
     4741 gttaaattcc aagtaatatt taatgttcct ggattaacca tgtttttatg ccatctgtta
     4801 gatgtctgtt catctaaagg ctcaaagccg ctgactcctg cactagcaat ttttccatca
     4861 atgaatgtat ttttggtagc ttcgatactt tgcggttccc attgtgctct tccgacattt
     4921 tgatttaagt ttcctgctgc tgatgttcct aggtatgcac ggcttcctgg agaagtaata
     4981 taaccatgcg caaaacctgg aatgctaaaa ccgaacaacc ctaaactaac taacccaata
     5041 aacaataacg tactaatctt tttcatgtag aaacctcctt catttgataa ccacattgta
     5101 aacgctttat tgtattaaca caaacattat gcttttataa ttttatgttg aagtaaaaaa
     5161 ggtgaaaaaa gtaagatgca gattataaca gaagtagacg agaaaagtac aaatagaaaa
     5221 catgagaatc aaatcatatg atctttttca aaaacatcat tcatataaac aaatgttcaa
     5281 ctttttaata acaacaatta tcaatttttt tcttacttca caaactaaat acaggtcttg
     5341 ctgtgagcct tcatggtaaa ctaatattat agaaaggagt tagtaaaatg aagatgataa
     5401 aagatattcg tttttgggtt tgtgttatag gaattgtcat attaggcttt ttatcagggt
     5461 tgcttagcgg aaatcccgga aaatattact attctttgca acttccacca tttgcaccac
     5521 ctagctggat atttgggccg atgtggacct tactgtatat attgatgggt atttctcttt
     5581 atttgttact taatcacaac aacaaaaaac agcgaaacaa tcttgtcggt ctttttgtga
     5641 ttcagttcat ttttaatttt atatggtctg ctctattttt taatttaaga aatattttca
     5701 ttgcagcaat cgatattaca cttttagtta ttttcttgtc agtgcttatg tatcagttgt
     5761 ggctgcatca tcgcctgaca atgtggctca tgattcctta ttatttatgg gtactattcg
     5821 caactctatt gaattattca atttattttt taaactaagc gctaaaaaga gacgtcctgt
     5881 aatttaacac atagaaaaag tgccacctaa gttagatttt ttgtctaatt taggtggcac
     5941 tctttattaa ccaaactatt tttttctagt acccacttgt tgttccatac tcattattct
     6001 ggtcataacc ataaccattt tgttcctgtc cagcgtaagg atcataactt tgagtatctt
     6061 gacttggtgt atagtactga cttgtatctt ctgtttgctg ataataggta ttcggatctt
     6121 ggtactgatc atatgtgttt tctgaatagg aagaataatc ggtgtaccct tcactattcg
     6181 atccatcacc gtttgtcgaa tcgccatctt ccgaatcatc aaagaacatc attgaagcat
     6241 tctcattttt caaattaggt aatgtatcgc ttgtttttat atttaattgt ttcttcagtt
     6301 cattttggat cgaaagaaga tcattttttc ctaaaatttg atagtagata tcattgatca
     6361 tttgtccttc gccttgcaac tggtcttgtt tgattgtttc aaacgccggg gtgtagttcg
     6421 tcgctacatc aagcatgtca tcccaatcta agtccgtcgt cacattgttc tcaactgctt
     6481 ttaaaatttt tctataattg cttacgccat ctaaacttaa aactttattg acgattttcg
     6541 tcactacttc ccgctgacga cgttgtcgac cgatatcgcc ttctggatct tcgtgtcgca
     6601 ttcgagaata ggctaaacct ttttctccat tcaatgtttg ttcacctact ggtacatgga
     6661 caccatccaa agtaaagtca attttattat tcactttgat gccaccgacc gcattgatca
     6721 aatcttccat gccatccaaa ttgatcgtta cataatgatc aataggaata tcgagcagtt
     6781 gctcaattga atccatggcc atttcgacac caccaaatgc gtaggcatga tttaatttat
     6841 ctaccgttcc ataacctacg atattcgtat aaatatctcg gtccaaactg atgatcgttg
     6901 attttttttg tttcggatta actgttacta ccatcatcgt atcagaacgt ccttgttccg
     6961 ttcttcctaa tccaccggta tctaaaccta atagcaaaac cgaaaaaggt tctttgtcat
     7021 caatgcttac tctctcagaa cgtttggagg atattcgatt agagttttta gaaattttgt
     7081 ttactgtttg gctcgcttct cccataaatc tgattccgta tgctgataat ccggcaatca
     7141 gcaaaacgaa tacccctaac aaggttaata gaactttttg ccatcgcttc ataatacacc
     7201 tctgtttcac attttctaaa atattgtctc atataactgt tcaatcagca aattcacttt
     7261 ttaatcataa gaaaacttta aaaaacattt ttgtgtaacg attattattt tttaaaattt
     7321 ctcaaaattc ttaactatca taccataccg tgaaaaagat cgaaagaccg aatacacgca
     7381 gttcgcaatc gttttatctt tttttatgta ttttttccag aaaaagcgaa caacaaggta
     7441 ttttccaaat aatcgtgcag taaaatgatc gcacatacct ttcccatctt tttaacagtc
     7501 tttgcctacc gttcagatta tcattcgatt tcacgacaat ttcttcctat gactggtcct
     7561 tttgttaaac ccatcttcta ttcgttttat ttccagctca ctgcatggct cttaaaatga
     7621 ctcgaactag tgattgcatt accatcatct tttaatggat agatatgctc ctcttcaaag
     7681 taaaacgatt ttcttttaaa gtaatagaat tgacatggta atgagtatgt atactagtgg
     7741 tttcacaaat ggtcttttat tcaacatcca aaaactgaaa tttctttaaa atccattact
     7801 aacgaagaaa atgttccttg cttgataatt agttaggaca tgtcaagtga acaaatgcat
     7861 ttacttagcc aaaaaatcta aaaacttcat aaatagacaa aaagacacga ctctctaaga
     7921 agtcgtgtct ttattcaaca aattattata atttagtcac ttcagcagct tgaggtccgc
     7981 gtgcaccttc aacaatcgtg aattctactt cttgaccttc ttctaggctc ttgaagccgt
     8041 ctccttgaat tgctgagaaa tgaacaaata cgtcatctcc gccgtctact gtaataaacc
     8101 caaatccttt ttcattgttg aaccatttaa ccgttccgtg ttccataaat gtatatcctc
     8161 caaaaaagtt cttgcaagta accttgcaag aacattatac ccaatttaga aaacgctagc
     8221 aagctttcgc tcttttattt tccaaattat ttttaagact gatatgacgg aggaaatggt
     8281 tattttgctg cttcgaaacg tttgtttact tcatcccaat taataacatt ccagaatgct
     8341 gcaatataat ctggtcggac atttttatat ttcaaataat aagcatgttc ccaaacgtcc
     8401 aaacctagaa ttggtgtctt tccttccatc aatggagaat cttgatttgc agtagaggta
     8461 atagctaatt ttccattttc cattacaagc caagcccatc cagaaccaaa tcgtccagcg
     8521 gctgcttttt tgaattcttc tttaaaagaa gaaaaatcac caaaagcttc attaatcgct
     8581 tcttttattt ctcctgtagg ttcgccacca gcatttggtg ccatgatttc ccagaaaaat
     8641 gaatggttag catgtccgcc accattgtta cgtacagctg tcttgatatc tgttggaata
     8701 gcgtccatat cagataataa ttcttctatt gttttttcgc ctaattctgg gtatttctca
     8761 attgctgaat ttaaattcgt cacataagta ttgtgatgct tatcatgatg cagatgcatc
     8821 gtttcttcgt caatataagg ttccaatgcg tcatatgcat aaggtagatc tggtaatgtg
     8881 taagtcataa aaaaattcct cctcaatgtt ttcttaataa tgtctacatt acaaagagta
     8941 acaaagagaa ggaaattctt caattgttat gcctagtcgt ctttttcact ttgacctact
     9001 tcttcttcaa aaacctcagt ttctttaaaa ataaacaact tgctgaaaat atagttcgca
     9061 aggataacaa taatttgagt gatgattttc gccacatact cattgctgtt cataacattg
     9121 atcaacaata ccattgcccc catgtccaat ccgtaagaaa ggatacggta gaagaagaac
     9181 ttaccgcatt caataagtgc atgtgtaaaa ttcggtgatt ttgaatggaa tacccaaagt
     9241 ttgttcgtta caaaagcaaa caatacagaa caaaaccacg aaatagaatt ggataaggcc
     9301 atacttaaat caaaatattg gcgtgcgaga gcaaaactaa caaaatttac aatcgtagcc
     9361 aaaccaccaa aaaagagata gactagtatc tcccaatatc cttttgcttc taaatatcct
     9421 tttaactgtc gatactgctt catgagccaa cctaccttat taaaaattct tatttatcat
     9481 actctatatt tcaataactt tcatctttat ttaaaaaaat taattttttt tgatcctttg
     9541 cctttgtctc tgcttggatc gtcacatgtg taataccata ttttaatttt aataaagaaa
     9601 ttatacgagg atatatcttt tcaatctcgc ttagtagctg atcttcaaaa tcaatatgtc
     9661 cgtcgaaaat aattatcttt tcgctatact gccaaatatg gacatgatga acatccttta
     9721 ccccatcgat tgcttcaatt tcttgttcca ttttttcata gtcgatagca ggtgcacttt
     9781 gtatcaaaat agcggcagct tttttgatca ccattatcgc ttctatcaat atatatatcg
     9841 aaatgatcag tgtaataact ggatcgatcc agtagatatc gaaaaaacgg atcaaaattg
     9901 ctccgataat cacacttacc gaagacagtg tatcacttaa caaatgcaaa taactagatt
     9961 ttatattcaa actttcatct gcttcttgtc gtaataataa tactgagaac aaattcgcga
    10021 gcaatccaat aactgctact gttagcatca aatgactatt tatcttttct ggtgaagtga
    10081 accgcttgaa tgcttcaacg gttaaaaaca aggataatat gatcaatatg aatgagttga
    10141 tgaaagcact caatatttct aaacgctggt agccatacgt acgtttttta tttgcttctt
    10201 ttcgggccat tttttgtgcc ccgtaagaaa aaataattgc caatgtatca ctaaggttat
    10261 ggaaagcatc tgaaacaaga gacaaactcc ctgacataat ccctccaata aattctgata
    10321 aagtgatcac cgcattcaaa accactactc caaatagttt ttttgatgat ctctgtggct
    10381 gtgtaggcac tattccttcc tccattttat ttatattgga tagtctttag acaagaataa
    10441 catttttttg atatttcacc aatcattcag ctgtttcctt aaagcacttg tatttcactt
    10501 gatacttcgc tagaattgca agtgatgaca ctactagatg aggagtactt atgacaacaa
    10561 aacaattaat tatcagttcc tttatacgat tcaaagacct aaaggaggcg agaaatgtcc
    10621 cattttggaa agccattgtc tatcttttag ttttgagtat aattatggct ttgccaatct
    10681 cttttcaagt ttttcaagtc ttaaacaaca ttcaattgga tgggcaaaag attgctactc
    10741 gcattcctga ttttacaatc caaaacgggc agcttcaaac cgaagaaaaa gaaggattca
    10801 tttaccaaac aaactccatt atcttcacat ttgatccaga gggaaaacga acagaacagg
    10861 acatctctac tgatttgatg gggaattttg ttagtgtcgg gatgttaaaa aacaaactta
    10921 tcatagctct tcctaataca ggcacgactt ctgctttgtt gaacaataac caattagaac
    10981 ttccctacac caatgaatct ttaaaaaact taacaggtaa gcagctccgt tcttttttaa
    11041 gtgaagcatc tataccactt tggataaaag tcctgacatt tatattttct atttatccta
    11101 gttttctgaa tctgctgatc actttgctat ttaccaacat tgctgctttt ctctatgcac
    11161 gctttcgact aacaaaagct acttttcttg attgtttaaa aacattgatc tattcaattt
    11221 cattgcctac tataattgca acaatcttga tggtctttct tccttctttt gacactagcg
    11281 ccttcattgc ttttgcagga atctttattt ttgctcaagc tgtcaaagga tgggcaaaaa
    11341 tatcgatcaa ttaagaaata aagacaactc gttatttcta cttcaataga cccttttaga
    11401 ggttgtgaca tttttttgtc acagcccctt ttttatttga aaaatatttc tttctcaaaa
    11461 aacaagcaag gcgcttcaaa gttcttcttg tatgcgctac acttagagta aagaaaggcg
    11521 ggaaattaac tatgtcagaa agaatcattt atgcagatcg tccatttcgt gatgaattta
    11581 tcgaagaaat ccatactata gcgcctggtt atatctttaa aacagaatta caaccagagg
    11641 atctacaaaa tgtagagatc agtttgggct ggaactcaga ctaccaagaa aaactattag
    11701 cctcttctac cttgaaatgg gtccaatcta tctcagctgg tgttgatcag ctaccccttg
    11761 agactttttc aaagaaacat attttacttt caaatggtag tggcatccac agtgaatcga
    11821 ttacggagca tgtcttagga atcatccttg ggtattctcg tggtcttttt caagcacaaa
    11881 aggcacagag tgacatgaaa tggttaggtt catcggtcca ttaccaatct atccaaggga
    11941 aaaaaatact aatcattgga actggccata ttggaaaaat gattgcaaaa aaatgcaaag
    12001 cattagacat gaactgctat ggaatcaata catccggtca tccagcagaa ggaatggaaa
    12061 agactttttc tttaaatcaa ctaaaggaag ttcttcctga agtagatttc gtagtcaata
    12121 tcttacctct aacggaggaa acaactgggc tctttgatca gcatatgttt gaacactttg
    12181 atccaaaatc tgtattcatc aatgttggcc gtggtgcttc tgttaagaca caagatctgg
    12241 tgcaggcttt aaataaccag caactttcct ttgcagcatt agacgttttt gaggaagagc
    12301 ctcttccaca agatcatcct ctttggaaaa tggacaatgt actgattact tcccacattg
    12361 caggtttgac acctgatttt caaaagaaat taatggctat ttttttaacc aatctgaaaa
    12421 gctactttgc cacccatgag cttcaaacca atcaagtaaa attgtctgca ggatattaat
    12481 agctgaaaac aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct
    12541 ctttttttat tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac
    12601 aagtaaatca tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc
    12661 atccatttcc actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa
    12721 accatcatga acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc
    12781 tggcagatca atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat
    12841 cccaacaacc tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac
    12901 atcgcctgtc tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc
    12961 aggagaagcg ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg
    13021 ttgcctgaaa tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat
    13081 ttctgctaaa gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa
    13141 attatgcgca atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat
    13201 cttttcaagt actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc
    13261 catttccttt accatcagag taactaagat actggcttga atatcttctc caatagcgac
    13321 aattacatga tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt
    13381 tgccacaaca gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc
    13441 aatagccagt acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg
    13501 tccaagacct acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt
    13561 ctcagtataa caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa
    13621 gaaaaataat agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca
    13681 ccaaaccatg tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag
    13741 ccaatatatt ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta
    13801 tttcctgatt actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta
    13861 gttcttcatg taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca
    13921 cttctggttc taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat
    13981 gtgcggtaga ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt
    14041 tttcgtcatc aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga
    14101 ttgcttttgt tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt
    14161 ccggtcgaag tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta
    14221 cttcaatgtc ttcttgaata gataaataag aagagacgcc taagcgcacc atttcatggt
    14281 catctactaa taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga
    14341 tttctacact tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag
    14401 ctactcgttc tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa
    14461 accctgttcc gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata
    14521 cctctaattc attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc
    14581 ggaacaagtg atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg
    14641 aaatctggat tttattttgc aattccttca ataactgctc gataccttgc tttaaacttt
    14701 tctcttctaa attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag
    14761 cgttgatgat ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt
    14821 tttctgcttg ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa
    14881 ctgaatcatg cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg
    14941 tttgtccatc tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa
    15001 tcattttttc ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg
    15061 atgcacttgg gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac
    15121 gcagcttttc ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga
    15181 caattaatcc tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata
    15241 aacgtgcacg cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga
    15301 aaagtgaaaa tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc
    15361 tggatatttt tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat
    15421 aatcttcaat ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt
    15481 tttcaaagaa aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa
    15541 tcggatggct acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat
    15601 cacaatatta tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga
    15661 aatgacggcg atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc
    15721 gaataattgc tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat
    15781 catgatctgt ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac
    15841 acctttcaga ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt
    15901 gactaagcta aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt
    15961 acgaggatgt ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa
    16021 aagtccagta ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac
    16081 cacaaaaaaa cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc
    16141 atcataccta tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa
    16201 atctaaaata cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta
    16261 gatctttcag tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc
    16321 tggagtagca atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg
    16381 agaatcattg gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta
    16441 ctcttcttct tctccatctg gtagttcgat gaatgtaact gttttaccaa ttgatacctc
    16501 atctgcatct acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat tttcaattgt
    16561 tgtgattctt ccttcaacaa atgcttgttc atctttcgct gattcatatt cagagttttc
    16621 ggaaagatct ccgaaaccgc gggcaatttt gattcgttca atgatttctc cgcgtttgac
    16681 tgtttttagt tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata
    16741 aactttctct accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct
    16801 tttgaggaat atagtccaaa aaagattacc acgcttttct tcaaatgtaa atgtttttct
    16861 aaaaatgatc aatgtctaga gcctgttttt ctaagaatta tttacatatt tttccactaa
    16921 ttccatatgt tcttcataag ttttagcgaa ataaacattt ccagtgctcg tatctgctac
    16981 gaaataataa tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg
    17041 attgtcaaaa ggtccaggtc catagccggt attggtatac aaattatatg gtgaatctac
    17101 agctgtatct tcatacgtca ctaattcttt gtgttcacct aatgcgtata aaacagagat
    17161 atctgactga agtggcatat ttgctttgat tcggttaaag aagacttgtg caatgttttt
    17221 acgatcattt tcttttacgc cttctttttc aactagagaa gctagagtta aaacttcttg
    17281 tacagtcaaa tttttctgct tgatagctga ataatagttt tgcatgaccg tattcgtctt
    17341 gttcaccatt tcaatcacta gatccttaag cgaagtactc gtgtaataat catatgtggc
    17401 tgggaataag taaccttcca aacggtattt tgtgttctca gctttagatg cactttccaa
    17461 cagttctggg aatttttggt gcaactcgtt aaagaaagtc tcatcattca ctaaatctaa
    17521 aaattctttc ttgtctttac cagttacctt cgctacccgt tcagcaatct gatcgatatc
    17581 gtacccttct ggaatggcaa ttttcccatc tgcaactttt gttggctcag aagtaccgcc
    17641 ttcttgcaac tgtttgccaa tctcgtcaag agtcatatta ggcgctaact gataatagcc
    17701 agcttgaaag ccagttaaat ttttaaattt tgtataataa ttgaaaacaa tcccgctttt
    17761 aataatattg tccttctcca aaatctcacc aatctgcttg ttagaagagc cactcgggat
    17821 ctcgacttgg actaattggt catctgattt atctaaaggc ttcaacccgc tatctacata
    17881 acgatacacg gtaaatccaa gaaatccgcc aataatcaat aatgtaagag caatcactaa
    17941 aatgatcttc ctcacaatct tatcttcttt tttacgcaga tttctccgct tgtcgattat
    18001 atctgctgta gctgccttac tggcttcttt tttatcttta cttgggtgtt gtttgtttcc
    18061 tgacaaaaca actcctcctt actatcaagt tcgtaacttt attcattata catgatattt
    18121 gaattggcgt gaatgatttt atttaaatat tctatcaaaa atgtaagtag taaatccatt
    18181 actataagat taaaataaat gttatcaaga tacaagctat tttagcttta ttctttctgt
    18241 aaaaaagaaa ggagactaaa taaactatta tgaaaaaagg aaaacatgct aaaaaaagaa
    18301 aatattcatt tttaatgatc gttgtcgtgt tgtttagcct tagtgcgttc tctacaggaa
    18361 ctattcttgg atggcaattt tacaataatg aacactctca gatagttgcc catgctcaat
    18421 cagctcaaaa aaagttgata ctgactacca atgaacaaca gctcgttgaa aaaatcgatc
    18481 aactattaag aaaaaatgat tatattggct caatttacgt aaagaaaaat aatcgcatca
    18541 tcatggaaaa aggctatggc tatgcgaata aactcacgca acaaccaaat gatccttctc
    18601 tttattatca gattggttcg gttcaaaaag cattgactac tcttttgatt ttaaaacaag
    18661 tcgagcgtgg gcacctttct ttagatacta agctggctca attctatcca caaattgctg
    18721 gcagccagca aattactatc cgtgacttgc tgtatatgcg ctcaggcttg caccgaactg
    18781 cttctccaac cataccgatg actgatgaag aagtcgttca atttgcgatt cagcacttaa
    18841 aatttacaga ttatcagacc tctcattatg attcattgaa ctttaccata ttgactggaa
    18901 ttttgatcaa attaacaaat caaccgtatg aaaaattatt gaccgatgat atcatcgaac
    18961 ctttaggatt acagcataca aaattttatg accaagtcaa aaattcatcc aatcatgcac
    19021 tatcctatca aatgtctaaa gaggatgatt actttaaacc actgacagaa tctgaaacag
    19081 agatccataa cgaattaggg actggtaata tcagtatgtc cgtctatgat ttaaatacgc
    19141 tgtttacaca agttttatct ggaaaactag tttccaaaga cttactcttc tcattatggc
    19201 gtgaaaatgc gaatggacat ccatatagtg gtggggtata tagcggagat aattatatcc
    19261 tgtcgcaagg aaatatcgac tcttttgata catctgctat atttagcaaa gattcacagg
    19321 atgctatcat catggaaagc aatataaaag ctgacaaaac tatcaaactg cctactgctg
    19381 atctaagaaa ccaaatctat gacctgatgg aaggaactac taagttggca aatagctgat
    19441 atgatgaaaa gatagcaaat actacttctt tgaatagcat ttgcttaaaa aaagtactcg
    19501 actaaacgta ggaaagagtg tgggacaaat ctaaaaatca atttttgcta cagactttaa
    19561 ttccaaataa acagaacaga aagaggtgtt cttctttctg ttctgtccaa attattctaa
    19621 gatttctctt taccaacatt atttgacaaa gggcctatta aatctaaaat agtatcttta
    19681 gattgctaaa atatgtgcta gcaaatcatt catggcttgt tcttattttt aattttatgc
    19741 aataaaaaag aagaacaatt agcgtgatta tagaaataaa taatgagatt ttagaaaaaa
    19801 atgtgtatga atacctgatt gttactttat ttattccact ttcacttttt actgtaacaa
    19861 aaggacctcc ttgttttagt actgtagcat tttttgaatt aacagtaacc gttaatcctt
    19921 tataccggaa tacaggtaaa actattgttt tgccttttga agatttaaac gtaaatgtaa
    19981 tttcatcttt atttggagaa aagctactat aaataacttg accatcttct gtaacttgat
    20041 ggtttttcaa aagcaattga tcttttttat agcctatagg tacataatca aaattctcaa
    20101 attcttcaag tttattttcc atttgttcat tagaataagt tccttgaaaa ttcatttctt
    20161 tcaccatgtt aactactgat gaataattta aaattgaaat agctaatatt aataata
//
LOCUS       DARBDV010000055        19475 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000055 DARBDV010000000
VERSION     DARBDV010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.055"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(21..995)
                     /locus_tag="SCM56_001812"
     CDS             complement(21..995)
                     /locus_tag="SCM56_001812"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEG1110577.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1075..1950)
                     /locus_tag="SCM56_001813"
     CDS             complement(1075..1950)
                     /locus_tag="SCM56_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HEG1110578.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2080..3987
                     /locus_tag="SCM56_001814"
     CDS             2080..3987
                     /locus_tag="SCM56_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HEG1110579.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4528..7806)
                     /locus_tag="SCM56_001815"
     CDS             complement(4528..7806)
                     /locus_tag="SCM56_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HEG1110580.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(8077..8622)
                     /locus_tag="SCM56_001816"
     CDS             complement(8077..8622)
                     /locus_tag="SCM56_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEG1110581.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(8634..9326)
                     /locus_tag="SCM56_001817"
     CDS             complement(8634..9326)
                     /locus_tag="SCM56_001817"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEG1110582.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(9345..9629)
                     /locus_tag="SCM56_001818"
     CDS             complement(9345..9629)
                     /locus_tag="SCM56_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110583.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(9642..10205)
                     /locus_tag="SCM56_001819"
     CDS             complement(9642..10205)
                     /locus_tag="SCM56_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEG1110584.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            10433..11095
                     /locus_tag="SCM56_001820"
     CDS             10433..11095
                     /locus_tag="SCM56_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HEG1110585.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            11219..12415
                     /locus_tag="SCM56_001821"
     CDS             11219..12415
                     /locus_tag="SCM56_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEG1110586.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(12516..13892)
                     /locus_tag="SCM56_001822"
     CDS             complement(12516..13892)
                     /locus_tag="SCM56_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1110587.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(13929..14795)
                     /locus_tag="SCM56_001823"
     CDS             complement(13929..14795)
                     /locus_tag="SCM56_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HEG1110588.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(14884..15909)
                     /locus_tag="SCM56_001824"
     CDS             complement(14884..15909)
                     /locus_tag="SCM56_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEG1110589.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(15927..16601)
                     /locus_tag="SCM56_001825"
     CDS             complement(15927..16601)
                     /locus_tag="SCM56_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1110590.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(16785..17678)
                     /locus_tag="SCM56_001826"
     CDS             complement(16785..17678)
                     /locus_tag="SCM56_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEG1110591.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(17752..19449)
                     /locus_tag="SCM56_001827"
     CDS             complement(17752..19449)
                     /locus_tag="SCM56_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEG1110592.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLAKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         5683 a         4189 c         3446 g         6157 t
ORIGIN      
        1 tctttccctc ttttttttgt ttattcgaat tctttttctt ccagacggaa taacggactc
       61 atcggtgtat tttcatggat gcgtttgacc gcttcaccca ttaattctga acatgtaacg
      121 attgttaagt tttctggatg acgattttct tctgtgaaaa cagaatcagt gatacaaata
      181 tctttgattg gtgcattgtc taaaatactt ttcgcatgat cggacaataa tccatgtgac
      241 gcacaggcat atacttcttt tgcgccgttt tcttttaata catttgctgc tcgtgcaagc
      301 gttgctcctg tgttcaagat atcatctacc atgatacatg tctttccttg aacatcaccg
      361 atcacataac caccctcagt tgaagcatca tctgcatgat ctacgatggc taatgtgctg
      421 ttcaaatatt ctgaaagact acgtgcacgc tgaacaccgc tgttttttgg cgaaacaact
      481 acgatgtcat ctcccgtcag acctaattga cggtaataat gagcaaacaa cggcatcgta
      541 aataaattat ctacaggaat atcaaagaat ccttgtactt gaaccgtgtg cagatctaaa
      601 gtcagtactc gagttgcccc tgcttcttcg atcaagttag cgatcaattt cgcagtgatc
      661 ggttcatgag gtttcgccgt gcggtcttgc ctagcatacc catagtaagg caatacgaca
      721 ttgatcgttt tcgcacttgc tcgcttcatt gcatcgatca tgatcagtag ttccatatag
      781 tagtcattga caggtgcatt cgttgattga acgatataca catgatcccc tcgaatactt
      841 tcttcgatat tgatggaaat ctcgccatca ctaaattgct tcacgacgct tttacctaat
      901 tctgttccga acactttcgc gattttttca gctaatggac ggttgccatt caaactaaaa
      961 atcttcaacg tgtcatcctg atacttatta ctcatgtagt cctccccaaa acttctctat
     1021 tcttcgatag tttaacatat ttttccaaga ttttctgtta atttcagcaa attcttaagg
     1081 aagtgaaact gttccgttac tccaactggc aggaatgact ttatgataat cgccccagtt
     1141 gtcgtgtact ttgtaataac gttctcctgt ttcagtttca aaataagcgt acgctgtcac
     1201 ccaatgattg ccatatgtac tgccaagtac tttcaaaata ccaatcatca caggtttgcc
     1261 ttcacgtata cgttttgtag ctcgctgcca tgaacctaga agagttgctc gtgcacgctc
     1321 atgattgcca ttcttcttta aaaaactaga aatccctgta ctaacctgaa aaggaatcgt
     1381 aggaagtcct agtggttgga tctggctacg caatgccttc gtcaaaacca acgaatcagc
     1441 cgtatttttc ttgcgaatct catttggaag catccacccc ttacgatagt cttgataata
     1501 ggcaaggaga actgctgccg cgtaagtacc acaaagatag cctgaggggg tagcccattt
     1561 cttatacctc aaaaaacgtt cttctggcaa tcccacccag caatttgcga gttcctttcc
     1621 tgaccttttt tcttcgatca aaacgggaat atcttctttt ctgttgatcc atctagataa
     1681 ctcgattggc tcttcattga tcagcttcca ttttttacaa gaaagaccct cttgataagt
     1741 atattttgct ggagaatatt ctaccggatg tcctgattga tcgataatca cataactttg
     1801 atcttcaaaa accagagcta aaaaaagcaa tttacttttt cttgactgca gctgctgagt
     1861 gctggctaat tttttaattt ttttatctac tttgaaatat aaaggtttat ccactacttg
     1921 attcagttgg gatcgcctcc tttttttcat tataagtgaa ggattgaaaa aaacaaatca
     1981 gtttgattgt tcgaattttc aattttttat gttctatttg ttaattcctt cattttgtaa
     2041 gataatgggc acacgaaaca aaaaaggagc gatcgatgaa tgaacaaaga acgattgaaa
     2101 caagatttct atgaagcagt caatgaagaa tggcaaaaaa cagcaaaaat ccctgcagat
     2161 aaaccagcta caggcggttt ccaagattta gttgacggga tcgaccaact tttgatggga
     2221 gaaacagacg atctgcttgc ccatccagaa aaagtgtctt cagaaaaaat gaaacatttc
     2281 cttgcatatt atcaactggc aaacgattat gaccgaagaa atgagttgca tgctcaaccg
     2341 cttcaaccta taatcgaaaa agttgaatca ttgcagtctt tcgacgaatt gaataagcaa
     2401 tttccagaat ggctgttgga tacattacca ttgccattta atctggatgt agatgcagat
     2461 atgaagaatg cgcaaacgaa cgccttcttt gcttatccgc cttctttatt ccttccagat
     2521 aagacgtatt atgcatcaga ccatcccaac ggtgcacaac taataggcgt tttctttgat
     2581 atggctgttc agttattaga gcttgccggc aaagaaaaaa gtcaggcaga agcaatcacg
     2641 gaacaagcag tgcaatttga taaattgatt gctcctcatg taaaaagtgc agaagaaaac
     2701 gcagattaca gcaaaatgta taatccgcaa tcttttgata catttattaa atacacagat
     2761 aaactggact tgaaaaaact tgtcacgggt ttgattggga caactccaga taaagtgatc
     2821 gtaactgatc cagtgtactt cgatcatttg aatgacatcc tgacagaagc tcattttccg
     2881 ttattcaaaa gctggatgat cattaaaaca gtacgttctc ttagtggtta tctatcagaa
     2941 gaattccgcc aagtcagcgg aatctattct cgcgtattgt ctggaacaga cgaagcaatg
     3001 cctcagaaaa aagcagcgta ttaccttgcc tctggacaat tcgatcaagt agttggcgat
     3061 tactacggaa aaaaatattt tggcgaagct gctaaaaaag acgtagaaca aatggtgaaa
     3121 aaaatgatcg ccgtttataa aaaacgtcta gaagaaaatg actggcttgg ggatgcaaca
     3181 aaagaaaaag ccgtcataaa attaaataac ttaggtatcc aagtaggcta ccccgataag
     3241 atcccagcat tattcgacca gttcaaaaca gtacctgcag atcagggtgg tactttgctt
     3301 tcaaatactc ttgttttcag caaactcaca ttaaaagatc gtttcagcaa atggaacaaa
     3361 ccagtggatc gttcagaatg ggaaatgagt gctgacacgg tcaatgctta ttatcatcca
     3421 tttcggaatg tgatcgtctt tccggcagcg atccttcaag caccatttta cagtctagaa
     3481 caatcaagct ctgctaatta tggtggaatc ggagctgtta tcgctcatga gatttcccat
     3541 gcatttgata ataacggcgc tttatttgac gaatatggca acttgaacaa ctggtggaca
     3601 gaagaagatc ttacacaatt tcaaaacaaa gcacaagcaa tgatcgaaga attcgatggt
     3661 ctgccttttg ctgacggaaa agtaaacgga aaattaaccg tttcggaaaa tattgctgat
     3721 gcaggcggtc taagctgtgc attggaagca gcaaaaacgg aatcagatct ttctatcgag
     3781 gaattctttg tcaattgggc aacgatttgg cgtacaaaag caaaaaaaga ataccaacaa
     3841 cttttactgc aaatcgatgt ccatgcccct gctaaactcc gagcaaacgt gcaaccaaaa
     3901 aaccttgacg aattctatga aacatttggt atcacggaac aagacgctat gtatctagcg
     3961 ccagaaaaaa gagtgcgaat ctggtaagag gttgtgccag aagtggtcag cttcaagaaa
     4021 taagaaggaa tttgggaaaa tcgcttctca aatttttgtg acatttcggc ttatttttcg
     4081 aagaagttac ttctggaata ccatgtatta gaggttgtga aaaagctgct tagtttcaag
     4141 tagtaagaaa aattttgtga aaatggcttt ccacattttt gcataaattt gacttactac
     4201 cgagaaactg gcttttgaac accgtttatc gagaggttgt gaaatcagcg tcctgacctg
     4261 agtattagag aaaaaaacgg aaaaatagtt cctcatattt tgccgtttgt tgagctaata
     4321 ccgagggtca gctgattgaa caccgtttag tggagagatt gtgaaaaagc tgtcctgcat
     4381 caagtaataa gaacagccaa tcaaaagcag caaggagaac ctcatctata aaaagataga
     4441 aggaagtgag acatctattg gtctcatttc cttctatttt tactttcctc agtcaattgt
     4501 tttacatcat aaaaggagaa attccactta tttttttcgc tgtttcatga cataaacaac
     4561 tacaccgatt aacaaaattc ctccagctaa tgctagcaac gggttattaa attctcccgt
     4621 ttttgggagt ccttgagtgc catttttttc actttctttt gctgtcctat ccggatgaga
     4681 aggttttcca tttggttttc catttggtga ttcatttcct ggattttctc tttctggttt
     4741 gtcatttccc gggggcacat cggcagtttc tcgcacagtg atcgcttttt gctgggtaac
     4801 agattctgta tgagcagacc atttttcccc atcaaatatt tccgaacgat aagtgacttc
     4861 tagcgtatat tctccttttg aaggtgctga aaaaatagcg gtatgattag aaaattgagc
     4921 acttgactgt gtttcaacga tttggatctt ttctgggaaa taacgcatat cttcaaagac
     4981 aggcgttcct tgatttggtg tgaaagttga cacaaaaact gtaaaaggct tcagttcaat
     5041 gatatttcct tctgcattct ccgccacacc atttgcttct tgtataataa tcggattatt
     5101 ttgttcattt ggttttgtct gatttggttc ttgtacgatt gcatactctg gatattccga
     5161 agacggtgag gtttctccgt caaaaatagc acctacagat aatggaagac catccactct
     5221 ttccttttct gtttgacgat tccccggttt aatctgcatc agtccatgaa catggatact
     5281 tccattttca tagcgcgtaa caggctgctg accttctttc gaacatgtat ttagagactc
     5341 gatccaatct tttgatatgg tatgtccaag agtattttct gattgtttct ctcgttcatt
     5401 ccaatagtag ttcaacgagt ggtagatttg atcttcgttt tgattcaatg ggcggatctg
     5461 ctctctcatc tcaagattct ctgttcggaa agataagata ccagaagcat ggtagtctgt
     5521 gtttcctgct gaagaggtat aaagagccac attatatgaa ccgttattaa aacttgtact
     5581 gctattgacg ataatatctg ttccgctatt ggaatcgatt cctttcgcaa gattttcgaa
     5641 agacaaacta ttgatcaatt catgagggcc cgttaaagaa gaaccgccca ttttgaatcc
     5701 attaccgtta ccttctccct caattcctgg tacccagccg tttcgataag caactgagtt
     5761 tttgatgatt acttttccga tcgaacctgt ttcattttta gcaaaaaggt cccaaccgtc
     5821 atctgcatta tgatacgcga tacaaccgtc aaatacattt ccttctccta ttgtcagttt
     5881 ggccgcaaat ccatcggcat cttcaaatcc tggatcggca ttttcaaaag atgtacagtt
     5941 cttgactaag ttatgcgcgg gccatgcttc gaaaggatca tttgagctac cgctgatttg
     6001 gattcctgta tttccattcc gataagcatg aatctcttcg atcaaattat gatttccaga
     6061 aatctgtaat cctttttgca tagctgcact atttgtaatg tcaaaccctc gtaaacccca
     6121 gtagtgactg aacagtgtca tcccatttcc agttccttta aaatcaagta caggacgctt
     6181 gttttctgga tttttctctg caatcaactg aatcgggttt tctcttgtcc cgttaacgcc
     6241 ttttggaatc gtcaatcctc tttcgaagga ataagttcca ggcgccatga cgattgtctg
     6301 cccagcatat gcgtatctca atgcttgtgt cagagataat ggattttgtc tgcttccgtt
     6361 tcctgcatct gacccttctg gtgttacata aactgtcgaa tatttaggtt tacggtagtc
     6421 cactgtatga agaatctctt ttgtttcata actagtcatc tctacgtatt cttctggcgg
     6481 atagttttta tctggcgtaa aactgaaagt aaattcgttc gttccttcca tcagttttgt
     6541 ctgataatca aattcccgat tcgcagtgat agccaagttt tttgcgttct taagcacctc
     6601 tcctgttgct ttatttttaa ttgtcaataa tccgtcagca tttgctctaa aggacatagt
     6661 atgatcttgg tttcctgaat gattactaga ggtcacagtg taaatcggat caatcaagcg
     6721 agtaggtctt tcttgcccag gaggatctgt cgcaggatct gagtaagcaa cttcgattcc
     6781 ttctaccttg atcgtcatat tccttgctgc agcaaatcct gcataaataa ccgattcatc
     6841 tgaagcgtat aatttttccc agtcatacat gatgtcagaa gccatttctt ctcctgtttc
     6901 aggatcgtaa taatatgact ctaaaccagt attcgttttt ttcatctcta cgtccagttt
     6961 agtaatcgag ttcgtggccg gaaacaaatt cccatgaccg tttccgaata tattataaga
     7021 acctgtttct ttttccaaga accctgctga agtctctaaa ggtgttgttt ccactgagac
     7081 actcccagga gctggcgcag cttgcggatc ttcagatgat attcctgtga ttgcccgtgt
     7141 ccccaatcca agtcgcatcg tatatttgct gccgctcgtg ttgaccactt cttgtctatc
     7201 cgagtcccag acatattcga tccgactgcc tagtatggca aatgaatttg aataaaatgc
     7261 ctgcccaaac aaatgatttt ttggtactgc gtctcttacc attaaagcaa atccttcttg
     7321 ggcattcgta taggtccaag attctatttc gatcgtcgcc cgcatcctaa agttgatgtc
     7381 tttggggatt gcttgataat aataggtcat tccatctgat ccgcttgact ggaattttcc
     7441 gccgttattc gtggaagaga gccgcacgcc gtcttctaat ggttcaatgg catacctact
     7501 agcacttgtg ctttctccga aaacaacccc tttccactct tcatttgctt cttcagattg
     7561 cttttttgtc ttttcatttt ctgattcttt catcatagga tcatttcctt cttccgtatc
     7621 catatcttca ctagttcttg gtacttcctc cattgttgac atcccttcaa tgttcacgtg
     7681 tgcattttct tcgttcacag actgttgttc gttagcagca acttcaacga cagaaattaa
     7741 aaccggggta ctgagcataa ggagattcat cattagcgca ctgtatttta tacgtttttt
     7801 taacaagtgt attcctcccg taacaaatta gaaagcgttt acttaaagta ttacctaatt
     7861 tatttttact gtcaataaaa aacaaaatta taataagtgt attttataaa aagaaaaaat
     7921 ataacttaag ttgttgataa taaagagatt tattaacgta aaaagaataa gtttttttaa
     7981 aataacgtat aaaaaagagc ataaaacaaa gtcgtttgtt ttatgctctt cttttattat
     8041 tttgtgcttt ttattttatc aaatcacttt gtttttttat aaaatttctg cgcctagtgt
     8101 ggactcaaaa tgtctcaatg cccattcatg tccaacggga tcgaaacttg ccacggcatc
     8161 tttgaaaata tggagtttgt atcctagatt atacgcatcg actgctgtat gcaacacaca
     8221 gatatctgta catacgccgg tcaaataaat atctgtgatc tggcgttctc gcaaacggat
     8281 gtctagatct gttccactaa aagcggaata atgccgtttg tctatccaat aaacattttc
     8341 ctcttgctca tagtcttggt ataaactttt taaagagccg tacaattccc gtccacccgt
     8401 tcctatcaca ttatgcggag gaaataagcg gttctccgga tgaaaggaat cttttggatc
     8461 atgcgcatca atggcaaaaa cgacaaaatc cttttgatca ataaacttct ttgtatgagc
     8521 agtcagcttt ttttcaatta cttgtcctgc tgtcccagta gtcagctttc catcctctgc
     8581 aacaaaatca tatgtgtagt caatcgagat caatgcgttc atcttagtcc tccttatacg
     8641 agatgtttga caaattcgat caccatttct gctgatcgtt tgcctgcgtc ttcgataaat
     8701 tcgtcaaagc tttgtgtcgc cgagtgatct gctgtgtcgc tcattgcacg tacgatcaaa
     8761 aatgggatat tgaactgacg agctgtttgt ccaacagcgg ctccttccat ttcgcaagct
     8821 aatgcttcag ggaaattcgt taagatttct ttgattttat ctggcgagtc gacaaatgta
     8881 tcacctgtaa cgatcaatcc ttcttttgcg ttcaaattcg ttgcttttgc tgctttaacc
     8941 atttcactgc gcaaatatgt gcttgcttca taatatagag gcattcctgg taattgtcct
     9001 ggtttgtagc caaatcctgt tgcatctgca tcaaaataag ctactttgtc tgatataacg
     9061 atgtcaccta cttgtaagcc ttcaccgatt ccgccagctg aacctgtatt gatcaccatg
     9121 ttcacgccgt attgttggat caacagactt gtggtaatag atgctaagac ttttcctata
     9181 ccggaacgta cgacgatcac ttcgtggtta cctaatgaac ctgaaataaa taaagcgcct
     9241 gcgcgttccc atgataatgg ttcgcttagg ttctctctca aaatttttac ttcttcttcc
     9301 attgcgccga taataccgat tttcatcttt ttaattcctt ctttctatat aaatgcgatt
     9361 gctaaaaaaa ccacacataa cattaggata acaataataa gagatttcat caaaaaagag
     9421 ttccatttgg tcgtcttttc ccgttcgctg atccttgttt tcgtgattgg tttattcttt
     9481 ttgctttctt tacgataaaa gctggcaatc tttttttctt cttgctgata agctgtttct
     9541 gcttttcgct gtttttttaa tgactcgctt gcttgtgctt gttgtcgctt gcgaagttcg
     9601 cttcgcgtaa taagcggtcc tttggccata agccgatccc tctactttcc tttggtcaat
     9661 agttcccagt attgaatcgc ataaatcgtc tttgcatcac aaatcaactg atctttcatc
     9721 gcttgttgcg cctcttcaag agttagatga tacaactcca gtacttcgtc ttcatcttgt
     9781 gctaaaggat tctcgacctt ctctacccct tgtgcatgat aaatatgaag gacttcattt
     9841 gcaaaacctg gtgaaagata catcgaagta agatggctca gcgacttcgc acgatagcca
     9901 gtttcttctt ctaattctct agctgctgtc atttcgggat tttgtccttc tcctggatcg
     9961 atttttccag ctggaatctc caaaatcact ttttctaaag gttttcgaaa ttgtttgaca
    10021 agaagcagac gatcttgttc gtcaaaagca atgatcccga caccaccagg atgaaatacc
    10081 agttctctct tcgaagttcc tccatctggc aaacgaactt cgtctacagc aacatcaata
    10141 atttttccgc tatagatttc tttgcgactg attgtttttt cttcaaattg ctcattgttc
    10201 agcatatctt ctcctgcttt ctttgtaatc ataactgctt ttttttcatt tttcaatttt
    10261 tctcaaaaac tccttattag tttaccatag ataaacctta gattttctct aaatatagaa
    10321 aatccttctt tagtccgata ctctttctag tagtccgtat gctaaaataa gtatgtaatc
    10381 gaataacgaa aagaattcaa tactcaacga taaatgacat ggagggttta ttatgataaa
    10441 acgaaattgg ccgtggcttc tagcattggt cttgctattt ctctttgtcc gaaacaatag
    10501 tttttctttt gttctaggac ttttattgat aggtgtagtt ttagttttgt ggggactctt
    10561 tggttcgcgc aggaaaaagg gaaaacaaga agaaattcct gcactgactg acgaacttga
    10621 aactcactat gaaaaaagcg gtatgacagc gaatgaaatt tcattttttc gacaaacaat
    10681 gaatcaaaca aaaaatgaga tcaatcaatt gcaaatgaat atgcagcaga cagccaaact
    10741 taaagcaatc gatctccgcc atgatccagt caaagcagcg aaagctttat tcaaagaatt
    10801 ggtgaaagaa ccaaaccgac tgcatgaagc aagtcagttt ctttatacgc atctgccgaa
    10861 tctagttgat ttgacaaata agtataatga gatcaatgac catgagatca aaaacaaaca
    10921 gacctatgaa aaactagaag aaagcgcgca gatcatcgac cagctgtcag ctttaatcgc
    10981 acaagattat caaaaattcg ttgctgagga tctagatgat atcgacgtag agatatctgt
    11041 tgcgaaacag agcttaaagc gtgataataa atatgaacca gaacaaaagg attaactctt
    11101 ttgttcataa aagaagactc ggaacattca gatgatgtct ggctgtttcg agtacttttt
    11161 ctaaagtaga gacattcatg taagaatcga gaacagattc aacaaggagg aattttttat
    11221 ggatcataaa ccgactgaaa caaaagatgt tacaccagtt aacgatacac ttgaagattt
    11281 attgaacaac ccgttttcaa ctccaacaga tacgctgacc acttcacaac aagcggagat
    11341 tgatcagctc caagagcaac agacagctgc tcgtttaatt gacaaactac cggcggaacg
    11401 ccaagaacaa gctaaacagc tagcagcaaa aatcgatgct aatgatgcac agtctgtcat
    11461 cagctacgga tcggctgccc aagcaaaatt aagtgaattc tcacaatcga tgttgaatca
    11521 tgtgcaagca caagatattg gtcctgtagg agattcctta actgaactga tgtatcgtct
    11581 ccaagaagcc aatcctgacg aactgcgtgc cggagaaggg aacatcttcc agcgtgtctt
    11641 cgggaaggtc aaacaatcga tctatgaagt gacagcaaaa tatcaaaaga ttggtgcaca
    11701 aattgacaag atttcagtga aattagataa agaaaaagac ggtctgctaa aagataatct
    11761 gatgttagaa cagctttacc agaaaaacaa agactacttt gatgcactga atatttatat
    11821 cgctgctgga gaattgaaaa tggaagaact tcaaacaacg atcattccag aagcgatgaa
    11881 aagagcagaa gaaactggcg atcagatgga tgttcagatt gccaatgatt acacgcaatt
    11941 cctagatcgt ctggataaac gtacgcatga tcttcgctta gctagacaga tcacgatcca
    12001 gcaagcacca caaatccgtt tgatccaaaa cacaaatcag gccttggctg aaaagatcca
    12061 agcatctatc gctacagcca ttccgctgtg gaaaaaccaa gtcgtgatcg cattgacctt
    12121 gcttcgtcaa aaagatgcag taactgctca acgccaagtc tctgaaacaa caaacgactt
    12181 attgaagaaa aattcagaaa tgctgaaaat ctcagccatc gaaacagcga aagaaaatga
    12241 acgtggaatc gtggatatcg agacccttca aaccactcaa aatgatttga tcgaaacaat
    12301 ccaagagaca ttgcgtatcc aaaaagaagg aaaagaaaaa cgtcgtcatg cagaagtaga
    12361 gcttggacac atggaagaag atctgaaaca aaaactgttg gatttaaccc aatagatcac
    12421 ctttcgcttg ttactaaaac aaacgcccaa caacagttta ttacaactgt tgatgggcgt
    12481 ttttcttatc tttcttctaa cttctccatt ggagtctatt tctcattcat cttgctgtgt
    12541 ccatagccat agccaaaata aatgatcagt ccaataacca ttacgatacc gaacacgatc
    12601 catgtcacag tttgcagacg aagcatcaat acaatacaag tcaagatgga gacgattggc
    12661 aatagcggaa cgaatggcac tttgaattct ccagcttttg gtttgccaaa catttttctc
    12721 aacttcagta agcctagact aacaagcatc aaataggcga gcgccatgat attcgttagt
    12781 tccgctaata cattcaatgg gaaaaatccg gcaaagaata cagctgccac accagcggca
    12841 taagtcgctt ttttcggtgt acgatgcttt tgatcgattt ctgtcataaa cttaggaagt
    12901 aagccatcct tgctgacagc aaacagtaaa cgagcaagtg aatacatcat cgagatcgtt
    12961 accgtcagta gcgttaaaat tgcccctaca gcgatcaccg cgcccactgt tccatgtcct
    13021 acatacctca ttgcaaaagc cactgggtca ttgactccta gttcagtata cggaacgatt
    13081 cctgtcaaga tcagcgtcac gatgatgtaa aggacagtag caatcagtat cgaaccaatg
    13141 attcctcttg gcacatcttt ttgcgggttt ttgacttcct cggctgccat tgcaacggca
    13201 tcaaatccca agaaagcaaa aaacacaaga gctgcaccat ctagcacacc ggaaaaacca
    13261 aaaggcatga atggctgcca gttatcaggt ttaacgaaaa atattcctac aatgataaac
    13321 aacgcgatga taccaaattt gacatatacc atggcattat tcaaacgtaa tgctttcttg
    13381 gcttcaagcg acacgatgta agtgacgaat atcactacga gcaccgcaat caagtcaatg
    13441 tatgtaccat tttccggatt gtacccgcca gttaatgctt gtggaagttc ggcccctaaa
    13501 cttttcaaaa aaccttgcat atagccagac catccggaag caacagagga aaccgctagt
    13561 aaaaattccc cgatcaacaa ccaccctgtc agccaggcag caaattcacc aaatacaaca
    13621 tacagatagg catatggccc accaatcact ggcactcgcg aagcaaattc tgcgaaactt
    13681 agtccagaca aaatgacaac gattgcagct aagacaaatg aaagtgacag tgctggacca
    13741 gcattttgat tggccgcaac ccctgtcacc acaaatattc ccgtaccgat gatcgcacca
    13801 attccaagca tgatcaaatc agacgttttt agttcttttt tcatctcact cgtatgattg
    13861 agattgatct ctttcttacg aaacaaatcc attgaagttc ctccccattt taggttggaa
    13921 aacagcgatt aaactgtttt catataccct aaaaggatac cacctaaaat gactaaaagg
    13981 caaccaacaa taacataaat gagctctttt ttcgtttttc tttcgccaag gatgaaaatc
    14041 ccacctaaag tagagataat gatccccatt tgtgaaagtg agaagccaac agctaaacca
    14101 agtgatttga ctgttagaag catgcagatg tttcctaagc cccataataa tccacagatc
    14161 atgtttttcc acacaaactg atcgacttta actttacgga aagcaaagaa gcttgcgcct
    14221 aagatcattc cgatactttg aggtaagata attgacatcg catccaaatt cgcccagttg
    14281 acgatgatgg tatatacacc ataaccaatc gtagagaaga tcaatgctct aaaaccctta
    14341 ccaaaatcaa gcattttatt atctgtatct atttttcctt cgtcatcttg tcgggcagtc
    14401 aaatacgcac cgcttacaag aagagctaaa gcaataatac cgtatacata atctttcgtt
    14461 tgtgtccatt catggaagaa aacggctccc gcaatcgtat tcaaaattaa ttgaaaacca
    14521 gtagacaatg gtagccctac agaaacaccc atgtacttca ttccatggaa ctgtccattt
    14581 tgtccaacac tccaagctaa gccggataaa aatccgatca agaaaatcca aggggtgatt
    14641 acaggactaa caacgaaaaa gacaactaaa gaaaacagaa atgcaccgat cgtcattcct
    14701 agtgtttgtt gattggcatc gccaccaatt ttaccactta ctaaaccaat actaccccat
    14761 gccaacatag ggactaatgc tattaaaatt tccataacca atctcctttt atttcacatg
    14821 ataagaaact tatcagcgaa aacgttaaca acacaagcac tttttttcgt ttctaacgat
    14881 tttctaacgt ttttcattat aaaaatttaa gattgggaaa aataaagtca ttgttgtccc
    14941 ttttccttca gttgattcag ccgtcaaatg gatacctaat ttttcggcca gattttttgc
    15001 taaataaagt cctaatcctg ttgaatgtgt tttgctaagt cgaccatttc tcccagtaaa
    15061 tcccttatca aagatccgtt tcaagtcttc cgctgggata ccgatccctg aatctttaac
    15121 tgaaaggctg actccttgcg cttccttttc tatgctcaca atgatgtttc cgtgatccgg
    15181 cgtatatttg atggcattac taagcaattg gttgaagata aaagcgatcc atttagcatc
    15241 ggtcaaaaca gtatgatcct caccaacgat ctcgtagcga atattttttt ggataaaata
    15301 atttgcctga ctgcgtatca caggctgaat gattcctttt agtccatatt cttggatgag
    15361 gtagtcccgc gagaaactgt cgagtcttgc ataatacaaa acttgttcta cgtattcatc
    15421 gatttttgat aattcatttt ctagtaatgt atacttttga tcgtcaatat cgaactcgat
    15481 cgaacgaact agcaatttag aagcggaaag tggcactttg atttcatgga cccatgagtc
    15541 aatataatct ttttggtctt gttgattact gattgctgat tgcattactt cttggtgttc
    15601 acggatcagc tgattgatat attcttctac cagtttttct tcttctgtcc tcccaccact
    15661 caaataattt tgcaaagggg aagttttttg tatgtcggct aattttcccc accatcttct
    15721 cttgctcaag taaccaccca gcagataaaa aatcagaaag acgccctcaa tcaaagctaa
    15781 ataagcaatt gtgctcaaat caactttgat gtttggcgcc agccacatca caaagatcgt
    15841 caatgcaata aaaaacagcc atcccaagat caatgtccac tgatctttca aatatttaaa
    15901 aaaattcatc ttaaccgctc atcctttcag ggaacgatat atccctgtcc tacttttgtt
    15961 tcgatatatc cttggatacc tgcctgttcg atttttcggc gtaaccgatt gatgttgact
    16021 gtcaatgtat tgtcatctac aaatcgttcg tcatcccaca gtgctcgcag caatttttcc
    16081 cgacttaaga tctttccatt gtgcttcatc aaaataaata atagacgata ttcatttttg
    16141 ctcaaatcaa tgatctctcc gttgatttcc atacttccgt tgtcaacatt caacgtaatg
    16201 ccattgtggc tcatcatttc actagataat tctgtgtagt tgtaagaacg gcgaagtaat
    16261 gcattgattt ttgcaatcag tacatcgatt tggaaatgtt ttgtcacaaa atcatctgcc
    16321 cccatgttca tagccatgat catatccata ttcgtattcc tgctggagat aaaaatgatc
    16381 ggtactttcg atacttcacg tattttttga caccaataat agccatccaa aacgggaaga
    16441 ttaatatcta gtaatacaag ctgtggttct tctctttcaa agtctgagaa cacttgattg
    16501 aaatcggtcg ttccaaaggt ttcaaattgc catttctcaa gttcctccat gatcaattgt
    16561 cgaatgatct cttcgtcttc gactaccatg atttttgcca taaaaaatcc ctccatcttt
    16621 cttcttgcta ctacaatatc aaagaagccc ttgtgaaaca agttggctta cctggaaaat
    16681 gtcattttag caaacgttaa aaaaaactgg aaatatgcga tttaatcgcg tttgtctctt
    16741 ccaagcagct taaatttatt atgttattct taataattgg tctattatct gttatttttc
    16801 tttcgttgtt tttgtctttt aaattttcga taacaccaat aaccgaaaag cgttagcact
    16861 agcaatatac ctagaaggag tataagtaat cgataaagat ggtatttttt tgtctgttct
    16921 atctttgaag agacttcttc tgtaaaggga actcgtcttc ctgtcactag taatcggtga
    16981 gtgttcacgg tgtaaggtgt acaagtgata agtgtgacta gatcctgctt ttctctaatt
    17041 gctaatgtat ttgtttttgt tggaaggaca acaattttct caacgacttc ataagcgagc
    17101 atccgaccat acacttcaag atagaatcgg tcatcttgct ccatttcttc aagatccgta
    17161 aagagttttt tctcagcaat accgctatgg gcggtaatca cggaatgtgt actctctcct
    17221 ccagttggaa aagaggtgcc ttgcaggact gtcgctcctc tatctaacaa aagatcattc
    17281 gtttcatcaa ataaaggcaa gctaacattg attttcggaa tatagatggc tccgatcata
    17341 tgttgttcgt aatattcttt tcctggattt tggacatttc taaccgcttg ctcaaaagga
    17401 tctttgactt gtcccattcc tgggattact gatgtatgag caagcgtctg atttttcttt
    17461 tcatgcactg ctaattgttg ctcatgtttt tcttctttat cgcgttcaag ctgcttttga
    17521 taattctcga tccttctttg atccaaataa ttgctgagag catctgctaa aaatggatat
    17581 gtaaaaactg cagctccggc aagaaacatc actgccataa ataattgtaa taaaagactg
    17641 atttgggttt gaggtctatt acctattttc tttttcattt tcttcacctt cttatttaaa
    17701 aaaactgcag caacctttat aaaaccgatt gctgcagctt atttttgttg cttatacttc
    17761 agacttcatt ttagatttac ggtaccagct gtacgctcca atcatcaaac ttattccgat
    17821 tagtaagaat gccaagagcc cgttcccacc agtagcagga agtgacccgc gtttcatatt
    17881 cggtactctt tctggccctg gattttctac tcctacgatc tgaatttctt cattatagct
    17941 tccataagag atgataaatt cagtaaaggc tgcctcacct gttggaagta cataaccttc
    18001 tggcgccatg gtctctttca tttgataggt tccataatct aacccttgaa cctcaaagta
    18061 tccatttcca tctgcgacaa attctgttgc ttgtccgctt gttgcccact caattctatc
    18121 tgctcctgat tcataaacag ctaaaggcga ctccccttta taaaatattg cgtattcaga
    18181 acggtcttca ttccacaatt cgaatcttgc atctttaagc aattcactgc ttgatgcatc
    18241 atatttttca aactttcttc cacctgttgt gactttctct ggcggctcta ctccttcttc
    18301 ataatcaaat tcatcatttc gtccgatttc aataaccgct gtgttgttat ggaaatcatc
    18361 cgcttctagt tctgtggaaa ccttcaaatt atacgtgatc gttaaatctc ctccacgtgc
    18421 atctgctaat ctacctaaag tatctacttt ggctactcca ttttcttctg tcaaaatcgt
    18481 cacgacaaac ccgttgctta ctacttctac tatataatct tgtccttttg ttaaaatcgt
    18541 ctcgccggct cgcacttcta tttcctgatc ataataagtt agaccaactg taggcgtatc
    18601 tgtgattttg taatttttga taacagcgct tccgttttca aggacagaat cgatgaaata
    18661 aggaataggt gctgtgatcg tgtaagaagc cgtttttcct ctttctagat caatataaga
    18721 aatcgtttct tgttctccag cttcattaat aatcgttact tgtctaaggt cgctttcctc
    18781 tacattcatc tgtttatctg cttctctgat ttcgtttttc ggatacaaat gcacatctgt
    18841 attgtatgtg tctttccatg tagaaccatc ccacacgcca tccaccatat caggcatcat
    18901 cactggcatc ataacaacca ttggagacgc gattctggaa atatgagctg gcgaataagt
    18961 ttctaagaaa agatagactt tatctctaga agaaggtgct tcttccgtca cttggacggt
    19021 agagaatacc gcttctccag cttcattcgt tgtttgtttg tcaatacttg tcagtcgata
    19081 ttttaaaaac caactttctg tgtgccgttt ttgaatccat tcaatcgctt cggcagaatt
    19141 aagaccgtct tctcgttccg gtgttaaagg attatctcga atcaaatcgt aatatacttc
    19201 cgttacgtca aacaaatcaa aatcgactcc tggaatattt tcttcaccaa aatccgtaga
    19261 aaccaatccg ctattcggtc gttcttccgg aacagatgag aaaccttttt tgtgtaatgt
    19321 aatcgtcact tcacttggtt tttgtgcgac ttcttcggct ttggctagtt ttccgttccc
    19381 gaaaatccct attcccgcag taacgagtaa cagtacactc attcccgtga caatcatttt
    19441 tttattcatt tttttctccc cgttcttttt ttatt
//
LOCUS       DARBDV010000056        19338 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000056 DARBDV010000000
VERSION     DARBDV010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.056"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(407..766)
                     /locus_tag="SCM56_001828"
     CDS             complement(407..766)
                     /locus_tag="SCM56_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HEG1110593.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(796..1137)
                     /locus_tag="SCM56_001829"
     CDS             complement(796..1137)
                     /locus_tag="SCM56_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110594.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(1134..1808)
                     /locus_tag="SCM56_001830"
     CDS             complement(1134..1808)
                     /locus_tag="SCM56_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEG1110595.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="SCM56_001831"
     CDS             complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="SCM56_001831"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEG1110596.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(4322..5512)
                     /locus_tag="SCM56_001832"
     CDS             complement(4322..5512)
                     /locus_tag="SCM56_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEG1110597.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(5533..6060)
                     /locus_tag="SCM56_001833"
     CDS             complement(5533..6060)
                     /locus_tag="SCM56_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEG1110598.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(6107..8896)
                     /locus_tag="SCM56_001834"
     CDS             complement(6107..8896)
                     /locus_tag="SCM56_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEG1110599.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNGKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(9043..9243)
                     /locus_tag="SCM56_001835"
     CDS             complement(9043..9243)
                     /locus_tag="SCM56_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG1110600.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(9696..10016)
                     /locus_tag="SCM56_001836"
     CDS             complement(9696..10016)
                     /locus_tag="SCM56_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110601.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(10519..11637)
                     /locus_tag="SCM56_001837"
     CDS             complement(10519..11637)
                     /locus_tag="SCM56_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110602.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            12108..13415
                     /locus_tag="SCM56_001838"
     CDS             12108..13415
                     /locus_tag="SCM56_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEG1110603.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            13536..14735
                     /locus_tag="SCM56_001839"
     CDS             13536..14735
                     /locus_tag="SCM56_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEG1110604.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(14762..16030)
                     /locus_tag="SCM56_001840"
     CDS             complement(14762..16030)
                     /locus_tag="SCM56_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HEG1110605.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     gene            complement(16200..16841)
                     /locus_tag="SCM56_001841"
     CDS             complement(16200..16841)
                     /locus_tag="SCM56_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HEG1110606.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(17250..17753)
                     /locus_tag="SCM56_001842"
     CDS             complement(17250..17753)
                     /locus_tag="SCM56_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEG1110607.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(17876..18640)
                     /locus_tag="SCM56_001843"
     CDS             complement(17876..18640)
                     /locus_tag="SCM56_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEG1110608.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            18748..19023
                     /locus_tag="SCM56_001844"
     CDS             18748..19023
                     /locus_tag="SCM56_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEG1110609.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
BASE COUNT         5539 a         4122 c         3443 g         6234 t
ORIGIN      
        1 ttcttccata cagatgtttt ggcttattct ttctttttct cttcgatcat tgcccctgct
       61 cttatccaac ccgcacttgc cttcttgatc ttgacaggat agcaactaag tttgaagcct
      121 gttgctggca atttgtctaa attcattaat ttttcgatag gacaatatct tttttcaatc
      181 cctgcaaaat gtccttccca gatattcgaa gctccactac gctttcaaaa atatagaaag
      241 agttctttaa tgaaaattat ttcaactttc cataaacagt aatcccatat aaataaaaga
      301 cgtaaaagtc acatgggaga acttatacag gaaaacacat agagtcaggt gaaatacatg
      361 tatcccacct gactctatgc gttttctaac ctttattatt ctctttttat acaatttctg
      421 caccgaatgg catcaacgcc aatcgagcaa gtttgaaaga ttgcttggca aaaggtatcc
      481 ctatgatcgt gatacataga aggatgccgc tgacgacatg tgcgagagca attggtaggc
      541 ctgaaacgat cagccagatg atattaacga tcaatgacat gccagactgg tgataaatta
      601 cttctttgcc aaaaggcgca aaagaaaggg aggcgatctt aaaacattgc ataccgattg
      661 ggatacctat gatcgtgata caccaaagta tccccgctgc taaccagcta agaccaccga
      721 ttagtccgcc gaatataaac cagatgacat ttcctaaaca actcattgta tttctccttc
      781 tattactgtt tttgattaaa actgggcaaa aattgatttt attcttcttt ttttagcttc
      841 cagcatcaag tattctgcaa attttttttg gatttcttcc gtattttctt ttgttactaa
      901 tgtgtgttgg attagttttc ctgatggtcg ctgatccacc cgttcttctg actgttgcac
      961 gtccgcttgc gcaaatccga taatccgttt ttcttttgtt acagcaacga gcaatagttt
     1021 attcttatca tttaggtcag ctgccgtttt tgcttttacg aatgattctg gatgatcagt
     1081 aatcagctga ttgatttgag gtaaatcttc ttttactgcc caccgtattt ccatcatttc
     1141 tcacttttct ttcgctcatt tttatctcca ctaaaaaaat cgactgcggc aattgcatct
     1201 atccagcttt gtgcaatgat ctcatgtccg ccaatcgttg ggtggacccc atcttctctt
     1261 gtatatttcg aaaaaccata agcaatccct aaagcattga atatgccatc taaaggaacc
     1321 agcaatgcgc catattcctt agcaagttta cgaacaattt ggattttagg gtctaagtct
     1381 ttacgccagc catgacggtc gtcggttata ggaagtacaa acggctccat caagatgatg
     1441 ttgcagattt ccgctgtttg aagttcattt agtaattttc gataaacgga ttcaaattgc
     1501 tgcgcagatt tctcagttcc gaatatagcc tgatcgctag tattatgcca ggtatcattg
     1561 attccgatca aaatcgatac atagtcaggt tgtaaagcta aacaatcttc ttgccagcgt
     1621 acttgcagat ctgcaatttt gtctcctcct atcccacggt tcatgcattc aaatttcatc
     1681 gttggatatt gggagattag ttttccggca atcaaaagtg gataaccttt acctagatca
     1741 gttgcttctg ctcggtctct tcctgcgtct gtaatgctgt caccgataaa taatatacgt
     1801 tttttcattt gtcaaaaact cctttttgaa taaaacgatg attttcttcc ttatattatc
     1861 ccataagccc tatcaagacg aaagagaaaa tatatgtata aaaaatgaca ccagcctagt
     1921 cccttcaatc aattgaatga acgctcatca agtgtctagt tttttatact ttttttctta
     1981 acgtgccgga ttccccttga ttccttcgtt gtttggtcca gagaaatcga tcacacctaa
     2041 gcttccgtca atcagaccac tgatgacgcc atcagaacat atatttatag gctcatctaa
     2101 gacttccagt aaatgttctt ttactaatat ctcttctaga ttgcttggga tagattggtc
     2161 ataactttat ctggtccgat gatttcaagg atgaattaaa cttacatatg gatcattgca
     2221 tgctgaaaat acggacctac tccatcttcg aatgttacta ttagtttgtt ttcaggtact
     2281 agatgctgct ttatttcaac agcagaagca tcatcaaatc agatttccat ttgaataacc
     2341 ttctttgtat atgtttgatt gattgcgatt tcgttataga accttgtgaa tcaaatagca
     2401 atttcttttg ctttcttctc ttgtctgctg gaaacttcct ttatcccgta ttttaaaaaa
     2461 agaactgcga gagttcgcag ttcttttttt taaataaacg ttgctctccg gccaactcct
     2521 cggtcacaag tcacaatagt catcaaacat gaaaaactgt tttttgaaat tactccttgt
     2581 gactcggagc tgaacggcag gcgcacaacc tcttgatata aacggtgttc aatcagctga
     2641 ccctcgatat tagctaaaaa aacggtaaaa tatgagaagc tatttttccg ttttgtttct
     2701 ctaatactca ggtcaaggca ctgatttcac aacctcttcc ttaaacggtg ttcaaaagcc
     2761 agtttctcgg tattaagcca aatttatgca aaaatgtgga aagccatttc cacaaaattt
     2821 ttcttaatac tcgaaactaa acagcttttt cacaacctct ccactaaacg gtattcaaaa
     2881 gcttacatct gcggtaataa gtcccgccaa ttcaaaaaca tgaacagctg tttttgcttg
     2941 gctgttctaa ttacttgatg cagaacagct ttttcatcac ctcttatgga taaattcggt
     3001 tcaacaaacg tgggaatgga atagcttcac gtacatggtc gataccagca atccaagtga
     3061 ctgttcgttc aaggcctaga ccaaaaccag agtgcggaac agaaccgtat ttacgtagat
     3121 caagatacca tgaatattct ttttcatcca aaccgtgatt acggatttgt tctagcaaga
     3181 aatcatagtc tgttgcccgt tcgcttccgc caataatttc accgtaacct tctggtgcga
     3241 tcatatctgc acaaattacc acgtcttctc ttgttggatg tggtttcatg tagaatggct
     3301 tgatggcttt tggatagttt aggataaaga caggctgttc gaatgaattc gcaatgaacg
     3361 tttcatgagg tgaaccaaag tcttcgcccc attcgatgtc atcaaatcca tttttcttca
     3421 gtaattcgat tgcatcatcg taagtaatac gagggaatgg caattttgta taagttttta
     3481 agacttcttt gtctcgtcct agtacatcta atgcatattc gcaatgatct aaaacatttt
     3541 gtactaagaa tgctacatat tgttcctgta cttctaaact ttcttcttga tgagtgaaag
     3601 ccatttctgg ttcgatcatc cagaattcga tcaaatgacg tcgtgttttt gatttttcag
     3661 cacggaaagt cggaccgaat gaaaagactt ttccgaaagc catagcagca gcttccatgt
     3721 ataattggcc actttgagat aaataagctt tttgatcaaa gtaatttgtt tcgaatagat
     3781 ccgtagtgcc ttctggtgca gaaccagtca agatcggcgg atcaattttg acaaaatgat
     3841 gttgattaaa gaattcatac gttgcacgaa tgatttcatt tctgatctgc ataatcgcat
     3901 gttgtcttga agaacgcagc cataaatgac gatgatccat caagaagtct gttccgtgtt
     3961 cttttggtgt gatcggataa tcgtggctct cgcttaccac ttccacattt gtaattccga
     4021 tttcataacc aaattttgaa cgactgtctt cacggatttc tccagttact aacacggatg
     4081 tttcttggtg tagattttta gctgtttgga aaacttcttc cgacacttcg ctttttacaa
     4141 ctacaccttg gaaaaaggct gttccgtctc ttaattgtaa aaatgcgatt tttccgcttg
     4201 atcgcttatt tgcgacccaa gcgccgattg ttactgtttc tcccacatgg tttttcgaat
     4261 cgatgatttg aatttgttcc acgtatgact ctccttttat attccttgaa tttcttcagg
     4321 attaattttg acttttactc tcaataaatt gcttgattct gcgtaccgct tcttctaacg
     4381 tagcgcgatc agtggcataa ctcatgcgga cattttctgg tgccccaaag ccttcgccag
     4441 tcacaagcgc tacccctgtt tcttctaaaa gatcttctac ccattgtgtg acattttcat
     4501 acttacacat gcgtagcgtt tcttttacat taggaaaaag atagaaagct ccttgcggct
     4561 tttcaagaga aacacccggc agttctgcca caagcggata taacgtattc aggcgttctt
     4621 caaaggcgtt ccgcatttct tctactgtat cttgctcacc tttcaatgct tcaacagctg
     4681 catattgact gactgctgtc ggattgctag ttgattggga agcaacagcg atcattccat
     4741 tgatgatcac aggatttccc accgcataac caatgcgcca gcctgtcatc gcatatgttt
     4801 tagacacgcc gttgatgatg atggtctgat ttttgattgc ttctgaaatc gttgcaattg
     4861 gagtaaactc attgccatta taaactaaac ggccataaat atcatcagca actatcaaaa
     4921 tatcattttt tactgcccat tcaccgattg cctgcagttc attcttgcta taaatcattc
     4981 cagtcggatt cgatggagaa ttgatgatta cggcttttgt tttttctgaa cgtgcttgtt
     5041 ctagctgttc aacagtaact ttccagctat tggattcttc ccctttcaca aagacaggaa
     5101 gtccttctgc cagttttact tgctcaccgt aactgaccca gtaaggcaca ggaatgatga
     5161 cttcatcttg cgggtcaagg atcgtttgga aaagggtata caaagcaaat ttcgctccgt
     5221 ctgttacgac tgtttgtgaa ggttcataga caagtccata gtttttcttc agataatcaa
     5281 cgattgcctg tcgaagctct gggattcctg ctgttggtgt ataatagcta gctttcccat
     5341 tgcggattgc ttcgattgct gcttcttgga tgttttctgg tgtcgcaaaa tccggttctc
     5401 ccacagtcaa actcagcaca tctctgcctt ttgcttttaa tgccttggcc tttgccgcag
     5461 ctgccaaagt gacagacggt tctaatcgtt ttgttctttt tgataactcc atcccattca
     5521 ttcctttacc ttttaaattc ccttgattgt cttgacttca tctccagatt caaagccaat
     5581 caggtaataa ttcagtttcc catttgtttc ttttcctact acttcccaca caggctgatc
     5641 atcatacatc cctaaagcag ctttttcgat ttggatttca ggatgggccg cagcaattgt
     5701 ctgttttgct tcgttttcag tcagtccctt tgtttgatcc aatactttta ctttttcacc
     5761 agatttagga atgataacgg cgatttcttt tcctttatca tttgtacctg tcagtgaaaa
     5821 gtatgtcttc tttcgagtaa accaataaaa atgatcggtt tcctcaagat ttgcatattt
     5881 ctttgcgatt tctactgctt ctttcttcgc ctgcaccgcc ggacgactgg aacgcaagta
     5941 aaaaatcgag ctgatcacga tgaccgctaa taaaaatacg atcaagccaa gtaacacgcg
     6001 attaatcgtt ttttcctttg tctgtttttt cttcaagggt cgtcgctcct ttatcttcat
     6061 tccttttcat tatagcagat tttaattatg aaaaaagaca gctcctctaa cttccttctt
     6121 catttttctt taagaattca tggatatcct gatagatttc ctccatagct agctctttga
     6181 tcggtacttt tttcggtaat gcatggatga tccgtgcgcc atattttgca gtgactagtc
     6241 ttcgatccaa aataagcaat acgccacgat ctgtctccgt ccttatcaag cgacctaatg
     6301 cttggcgtaa cttcaaagct gctttaggta cggattcctg gtaaaaagga ttgatcccct
     6361 ctgattctaa ataagcgttc ctagctttga ccatcgggcg aagcggatta tcgaagggta
     6421 aacgtgtgac tacaacgatc tgcaaagcat ctccaggcaa atctacacct tcccaaaagc
     6481 tgtcagcgcc aaacaatacg cttttttctg acaaaatgaa gcgtttcaaa agcttctccc
     6541 ggcttccacc aaacccttga gctagtattt ctcgtccttt ttctagcata gacagatgca
     6601 ttttctgata gaccttctgc aaaacatcat gagaagtgaa taacacaagt attggccgat
     6661 cttcttgttc gatcagacct gtaagtgctg tctctaaata atcgatatat gcttgattgc
     6721 tgacttgatg gacggcaggt gcctcttttg gcacatagat ccttgcctgt ttctcataat
     6781 cataaggaga ggcaatcact ttaagaggtg ctgcttcaat gccaagcctc ttagagagat
     6841 agtgtcgatt agggccgatc ttcagcgttc ctccaatata aagaatacga tcatatcgag
     6901 gataccaaac agtcgacggc aacatagccg cttgaaaatc atggagatga aaaatcatct
     6961 gctgcgctgt gttcccagga acgaaccaat ggatcaaatg atctcgccag tcttccagcc
     7021 attgatccat cagcaagtat tgcttgtcta attgatcaaa taggctatat aaacttgcta
     7081 aatcgatccg catctggttt gtccagcttt cttggataat ctcaaaagtc agacgcattt
     7141 gtgactgtag ctgctggatc tcctgataaa aaagctgcag gtgatcaatg gctgtcccag
     7201 attccagtgg cagctgatcc aacatctctt ttgtcatcat gacttcttct ggatactgtc
     7261 cgttaggttg cttgccgctc actaaactca ttaatccttc tgccatacaa atctgtgctt
     7321 cgcatagcgc agaaagttct tctcgataaa tcgataacca gcgtaaggct tcatgttctt
     7381 tcggtaaata gctttgtatt cggtcaaaca gctgatttgg ttcttctaat tgctggattt
     7441 gtttatggaa atagttaata cttaatcgtt tgccatttac tttttcagcg atttccggca
     7501 aatgatgtac ttcatcgatc aacaaaaatg gactagctgg aagcaatggt tcttttcgct
     7561 gtgtttcttg tactaaaaac gcatgattga caatcaaaaa attactttgc ctgattttct
     7621 tttgcaagaa acgaacaaag tcttgttcgt aaaatgactg atttcttgct aaaaatccaa
     7681 tacctctgtg cgcgatatct gcaaagaatg aatggttcaa atttgttaaa tgtaattcat
     7741 ccaaatcacc agtcttcgtt tgcgtcagcc aaactaagac cgccatttga tacaaagcat
     7801 attgtttttg ttctggcggt tccacaagtg tgcctttaaa acgctgcaaa tcaatatagt
     7861 ggcgatagct tttgataatt acaggttgta tgggctgatc tagcaattga ttcagcaatg
     7921 gaatgtcttt ttctatgatc tgctgctgca aaactagcga aacggtactg atgacagcgg
     7981 gtttttcagg tgtagccaaa taactcaatg gcagcagata accgattgtt ttacccattc
     8041 ctgtagctgc ttcgatcatc aaatcttttg attcatcttt tgtgaaatgg tcgtagaccg
     8101 cattcatcag tctgttctgt tcttttcgat acatcagtgt tttaccaaac attttttctt
     8161 ttgcttgttt ttttcttgga tatgtccgct cgccataatg gacggatgtg aatagttcca
     8221 cttctttttt ctgcaaagct agcccatcga cgatttctaa tgagggatca agtggctctg
     8281 gtttttcttt catttcttca atcacatgat aaatgaaacg actcgtatcc atacctgtgt
     8341 gtctacttaa tcgcgcaatc tgctctaacg tgatgattgg caattttctc atccgttctt
     8401 cgatcagcaa aagaagttga cctgtcactt cggcatcact atctgcctga tgaggattct
     8461 catgaataaa tccaaggctt tctgacaaat cagctaagcg aaagcttggt tccgtcggca
     8521 agaaaatttg tgcaagttct actgtatcga tgcctggtat cttcaaacta ggaagtccgc
     8581 atcgcatgag ttcatggttc aaaaaattat aatcaaaata aatattatgc gcgacaaata
     8641 tggtgtctgc caaaagattg taaatcgttt gggcgacatc ttcgaaataa ggcgcttttt
     8701 ggacgcgttg attcgtgatc cctgttaaat gctggatttg ttttgaaatc ttccgccctg
     8761 gatgaatatc gatcgaaaaa cgtgaagtga tccgacctga ttcaatcatg acgcagccaa
     8821 attggatgat ccgatcttct tttggattcg tcccagtcgt ttcgatatca acgactgcat
     8881 aagttcttgg tttcaaaata tcttcctctt ctcctgcctc taatgttctt ttgaaattat
     8941 actacaaagt gcagaaaaat actctcatcg ctttttaaag aaaatagaca aaaacgccca
     9001 gacacgcaaa tgtctgggcg tttatttttt agtataggtt aattaagctt tagtaacgtt
     9061 tgttgcttga ggtccgcgtt ggccttcttc aacatcgaaa gttactgctt ggccttcttc
     9121 taaagttttg aatccgtcgc cttggatagc tgagaaatga acgaatacat cgtttccgtt
     9181 ttctgcagtg atgaatccaa atcctttttc tgagttaaac cattttactg tacctgtttc
     9241 cataataaaa atcctccttg tgtatcaaca catattttgc aattacttga atggtgtata
     9301 tctggaagat gtttatttga aacaagctac cacagattac cgaacaaaaa ctactcctat
     9361 agtatatcat accgtttaca aaaaagctag agtttttcga ctttttttat taaaaaaagt
     9421 tttctcagcg attacataaa gtgaaaaacc gaactttttt ttaaaataaa cgacatattg
     9481 ttcagttggt gtttttttgc tctgatgttc ttttttaaac taaaaaaatt attctttctg
     9541 actacttatc ggttctgttt caacggtcga ggctggggaa gattctttag taggtctgat
     9601 gttctcatgg tatttctgat agcctatgac agttaccaga agaacaaggc aaaccaatac
     9661 gaccaacgag attagttttt cttttttgct catatctata cctcataaat aacaggatga
     9721 tgtccatcat atttctgagt atgagtaaaa gcaaaagctg cagctgtaga atgtaagaaa
     9781 tgccagcaac tttcataatc acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc
     9841 aaagaccgtt cttctgtttc aggaatcgca gaatcaagat ctggtgtctg tacataaaca
     9901 tttttgtaca gacgtgtgcc tgtaatagga aaccgctgtg tttcttcagc aaaaactggc
     9961 tgacttgtta aacttaggga tagagacagc aaacaccata acagtctttt tttcataaag
    10021 acctccttat cgataagttt ttcccaatta aaatcatatc tcaagaacgc ccgttattca
    10081 cattttgttc ataaatgatg ttttttgtat cataatcgtc aaaaagaggt cgtgaccacg
    10141 tcacaacctc ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt
    10201 tcacaaaaat ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact
    10261 tctgtcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca
    10321 ccaaacaaaa aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca
    10381 gctttttcac aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa
    10441 aaacggcaaa atatgtgaaa ctatttttcc gtttgtttct ctaatactca ggtcaaaaca
    10501 ctgatttcac aacctctttt aataacgaag aatcgctgtc agactctttt catctggcgc
    10561 atctttttga tccaaaataa acacttttcc gccattaatg atcacatcat ctgctaatgc
    10621 attcaacact tgacgccgat catattctgt ttcaggatct tcaccgaaac catcagctaa
    10681 gtttgacgta gcgataaata aatgtgacac ttttccttct ttcgcagctg gaacgatatc
    10741 aactaactga tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc
    10801 tgcgagttca cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc
    10861 aggagaagca gaaatcgcaa cgtctgcaca ataataggga tttttagcaa tttttttgaa
    10921 catcgtctga ttttctggta gagcaaataa ataaatcggc cattgttctg gattttcgaa
    10981 agaatccttc aagtacgtat caactgcttg gtaatagttg acccaatcga tctctacttc
    11041 ttcatctttt ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt
    11101 agaagagaag ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac
    11161 cggtgcccct tctggtagag atacttcttt tacttgtttg ttgctgactt catacaattt
    11221 catggagtcg cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat
    11281 aatagccagt aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa
    11341 acgatggata taggtttggt cagcagttat aatgaccgct acactcgtag ttgcactacg
    11401 ccaaaacgaa ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg
    11461 ttcggaatat tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat
    11521 ctgatctttt tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacgaatgg
    11581 tcctttagcg ccttctgaag tcaattgtga caataattct ttatcctttt ttgccataaa
    11641 cgtacccctc ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt
    11701 tttccataca ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc
    11761 tattttttta agttatcttc ataatttcct caattattta gccccagtct tcaactattc
    11821 ttcatgaaat gctgataaac taagaaaaag aataaaagag gtggatctta tgcatttgat
    11881 tatgattttc agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt
    11941 agatgcggaa acacagccta ttcctgtaaa agtacgtaaa aacgaccgct aaccttccat
    12001 aacgtgcgtt cttatcctaa agttttttca gaaacattga aactctctcc cctctctgat
    12061 aaaattttaa tttgtagcga aacaaattaa ttacaaggag atcggatatg tccataaaaa
    12121 aaacacgtct agtcctatta ttggctagtc tcattctttg ccttgcttct tttctaccag
    12181 ctgttgctgt gcaagctgaa gatactttca aagtaaatgc aaaagcagcc tttgctgtag
    12241 atgcgcaaag cggtaagatt ctttatgatc aagatggaga aaaaaccatg gggatcgctt
    12301 caatcacaaa aatcatcggt ctttacatcg tccttgatca agtaaaagaa gggaaactat
    12361 catgggatga taaagtttca atttcagatt atgcagaaaa tttgagtgtc accccagatt
    12421 tatcaaatgt tcctttgcat aaagaaaaca cctacaccgt taaagaatta ttcgactctg
    12481 caatcatcca gtcagccaac gcttcaatgg ttgccttggc agaaaaaatc tctggcagcg
    12541 aagcaaaatt cactgaacga atgaaagaac aattgaaaga ttggggtatc aaagatgcaa
    12601 cgatcgttaa tgcatcagga ctgaataact cttatcttgg cgaaaaccga ccagaaggta
    12661 ctggagaaaa cgacgaaaat caaatgtctg ctcaagatgt ggctatcgtt gcccgtcatt
    12721 tgatcttgga ttttccagaa atactggatg tatctagcac tacgacacaa atgtttggcg
    12781 aaaacacgca atcaccagta gaaatggtca actggaactg gatgctccca ggattcgtga
    12841 attataaaga aggcgtggac ggattaaaaa ctggtacgac tgaacttgca ggagcctgtt
    12901 tcgtcggtac gatcactaaa aacgatcaac gaatcatcac tgtcgtattg aatgcagacg
    12961 gccatgcgga aaacccaagt gcccgtttca atgaaacagc tcgcttaatg gattattgct
    13021 acgacaattg gtcagaaaaa gagcttggaa aagccaatgc aagtatccct agtctaaaaa
    13081 ccattgatgt aaaagacgga aaagaatcaa cagtgcccgt tgtgcttaaa agtcctgtaa
    13141 aagtatgggt acggaacgat atggatactg gaaaattaac aatcacacca actcttgaca
    13201 aaaaacaagt cactgacaat gagatccaag cacctatgga taaaggaaca aaaatcggta
    13261 ccgcaacgat caccttggca gaagataaat taggttatct tgaagacaac aacgcgccat
    13321 ccacagaaat catcacggat aaaaccgtag aaaaagcaaa tattttcgtt attggctggc
    13381 gacgcgtcgc agaatttttc ggtaacttgt tttaattgaa caaaaagaaa aaagcatctt
    13441 tccatgtgga agatgttttt ttgctttttc tagtttaaat aacagctttt ttcatctatt
    13501 ttcgttatac tgtataagaa aaaggagttg aatttatgaa tctaaccaga gatctgattt
    13561 ttttggttct ctatcttatt ggggtattct ccatcgttta ttggcgaaaa cagaaaaagg
    13621 atccttcctt gttcttttac agcagtacat ggacgtttgc ggtaatcttg acttattttg
    13681 tcgtgttgga aattttttat tcgacctttt attttttgat tcccttagtg tttttcagta
    13741 tctttgcctt ttcctatttt tcagaaaagc gaaggctgtt gaatggtctt ttattcaata
    13801 ttttcctcat tagttttggt atctacttgt ttgtcttgct atacgaaaca caaaatattt
    13861 ttcttggcgg actgattgcc ttgatcacga tcccgttact gcttgtcttt ctattcggca
    13921 tttatggatt gatcgttttt cttttttgga acggtgtcac agttttacgt agagagtcgc
    13981 attcattagc taatctttta acgttgatac tagccatttt tcttacattg tttcttgttt
    14041 ttgacttttt cttgttaaaa tatttaccac aatggataaa tgctttgttt ttctgcatcc
    14101 cattgatctt gatctatcta tttatcgtat tctacaactt tttgactgtc tcattcttgt
    14161 atcagttcaa ccgccctcga tacaaccaag acttcatcgt tgtgttaggc gccgggttga
    14221 tcaatggaga aacagtttct cctcttcttg ccaagcgaat caataaggcg attgcttttt
    14281 attgggcaca aagccgtgcg acattaaatc cgcctatcct attaatgtcg ggtggacagg
    14341 gagcagacga aaaagtacca gaagcaatgg cgatgaaaca atatgccatg gaacaaggaa
    14401 ttcctgaaag agatattctt gtcgaaacca attcgacaac cactttggag aatatgcgct
    14461 attcaaagga aatcatggat cagcaaatga aaggtcccta tcgcgcgatc ttcagttcca
    14521 acaattacca cattttcaga gctggtctct atgcacgaca agcgaaactg aaggcaaatg
    14581 gcattggggc aaaaacagct ttctattact tgcctaacgc gtttttaaga gaatacatcg
    14641 ccatcttgat gatgaacaaa aaaagacata tgatcatttg cggattgctt ttcgcaggtt
    14701 ccgcattctt atcactgatc actttacttt tctaaaaaaa caaggtgtga caaaacatat
    14761 gtcacacctt gtttttttct tttattctct tgacgattgg caagaatagt attccttttt
    14821 tgacggctac ttgctgtgca gaataaatac cgctgtttcc tgaacacata aagctgatca
    14881 agcagatcat aaagaagtaa gcagcagctt gtgaaccaaa taattcgatt cccatcacaa
    14941 aacaggcaat cggtgtattt gtcgcaccag aaaatacccc gataaaacct aatccagcaa
    15001 gaaaaggaac agaaacatgg agcaaaggcg ctagtactgc gccaagcgtt gcaccaatct
    15061 caaatagtgg tgtcacttct cccccttggt accccgcacc taaagacaag accgtgaaga
    15121 ataatttccc aataaaatca taagcatgtg cctgtccatt aaatgattcc tgcagtaacg
    15181 gaagactcaa cccaaggtaa cgttgtgttt gcaaaatcaa cgctgcggct acaactactg
    15241 ctccacctat cgtatttcgc caaacaggat gaggaatcca ttgcgcatac cgcgctttta
    15301 aaaagacgat cgaacggctg aatacccaac caatcaaacc aaaagcaatc ccagcaacaa
    15361 aaactttgaa gaacagcaac atactccatt caggaatcac acccatttgg taatgtagat
    15421 gagaaacacc atagctttct gtcatgaaat ttgcaaaaag cgcagcaaag aagctaggaa
    15481 atattgcttc tgttcggact ttaccgattg ccaacacttc taatccaaat aatgttccag
    15541 ctaaaggtgt gccaaataca gaactaaaac ccgcactgat tccactgata accaaaatct
    15601 cccgttctgc tttatccaat cggaataaat gcgctatatt atcagctaat gctcccccca
    15661 tttgaactgc tgttccttca cgaccaacag atcccccaaa taaatgggta gtgatggtcc
    15721 caaacaaggt caatggaatg agccgcaaag ggatcttctc ttctcctcca ttcccttgat
    15781 cgatcactaa gttattccca cgactagaaa gaccgccatg atacgcataa agataagcaa
    15841 aaagcgcgcc gctgactggc aatagataaa gtagccaagg atgtgctagt cggatatctg
    15901 tcacgaacgt caggctattt aagaaaaaag cagacaagct ccccatgaat actccgagaa
    15961 tcgctgtgac caccagccac ttcaacatgt acatcaaaat acgcactgga ctcggtaatt
    16021 gtttttccat aaaaaattcc tcctcaaata aaaaaacgcc tactaaagaa aggggctggc
    16081 cccagacttt agtaggcgtc attagctatc ttacgttagc atttaaaggc gaacaccatc
    16141 acctattcac ttccattaaa agatagcata tttttctgta aaaaacaata aaattatcgt
    16201 tatttcttta cacgttcagc aaattcatag aaaggagcca atttcgttaa ctgttgatta
    16261 cattgatcac tatcatgaca aatataattc ccctttttcg tatacgtgcc atcagaaccg
    16321 gctttcgttg tagaaagaaa gagcgagaca ttcgatgttt tctgacagat cgcacagatt
    16381 cccttattaa tcgttggtga taaagtaccg ctgatccctt ttaattttcc atcttcgtaa
    16441 taaaccatga attttttttg cgtccctgga tcattccaac cgatgtacgt atgtgcttgc
    16501 aagtcgattt cattccaagc gggttgtttg agttttttca cttttcggaa taatttctcg
    16561 atttgtggtt tgctcggctg cataaacggc tggacgattt gcttcagctc ctccaatgct
    16621 cgttctgctt gcgtttttgt cagttttaca tctaaaactg tcgttaaaaa cgtatcgatt
    16681 tctgatggtt cgtaacctaa tacatcgcga atcttttcag ctgctagagc ctgaaccgtt
    16741 tgtaccgttt ttgagtcatt gacagatgag tacgcattta ataactgctc tacttctctt
    16801 tttataaaaa aatattgata tggtttcatt gtttgttcca tgattttaaa gtcctttctt
    16861 ctaaccaaag tgcatacttt gtagaaaaaa gtgatcttct gcaaagtatg tgttacctga
    16921 agatcccgga ctcttttaaa taagatacgt tttgcatctt atcatcaccc cctttaatgt
    16981 tcattcttct atgtacatga ttcattcgat aaataaaagt aaaccgtatc ttgagcagat
    17041 agtcaactgc aaacagaaaa aggactgtga caaaagtcgt gtcaaagtcc ctttctacaa
    17101 acaaacggtg ttccagaagt agcttcttca aaaaataagc cggaatttca caaaaatttg
    17161 agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gacccacttc tgtcacaacc
    17221 tcaatatggt ctattattgg ttttacctgt taaatcgagg attcttttcc gaagtaacgt
    17281 tgccatctct tacgcatact tgctgacata ggctgagcgg cttctttgac tgcgcagtat
    17341 ttgtctacga atgttacgat atatccttct ttgtacttag gcggtgcaat cgttgcgccc
    17401 cacatgtgct tcaaaatgat atctcgctca agatccgaca aatcggtgat cttttctgca
    17461 ttctttacag cgattcttgg atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta
    17521 cgccaatcat agtagaacag atcatgcaaa agtccagcac gcgctgttgc tttagcattg
    17581 ccaccccatt tttttgcgag cagataacta tagtaagaaa cactgatgga atgctccaaa
    17641 cgagtggaat gaacatgctg tgtgtaattc gctaattttt tcactgcttc tgtttctaaa
    17701 agatcttcta cataagacat atattcttcg tcttcacgcc atttttctgt cataattatc
    17761 gatctgccac ctttttgaat ttcattttct tctgaaattg atctaagtat accaccagtg
    17821 ttcggattat tctagtcgcc ttggataaaa tagccaaagc ttaatagttc cttaatcata
    17881 aagaaaaatg ataaagaaca atccctgccg caaccccaac attcaacgat tccgcatttc
    17941 ctttcatcgg gatatatacg ttctgatcag tcaaagacaa gatttcctga cttaccccct
    18001 gaccttcgtt ccccatgatt attgcgaagt tatctgattt ttctactttg tctaaggcgt
    18061 gtgcttcttc atttaattct gtgccataca ctgtaaaccc tttgtctttc attaaaggaa
    18121 tcacttctgt caaatctttt tgcaacacag gcaaatgata attgcttccc tgcatgctgc
    18181 gcagcacctt tgtactataa atatctgccg ttcctttgcc taataaaaca ccagccatat
    18241 caaatgcatc tgccgtacgg atcatcgtcc ctacatttcc aggatcttgt acattatcta
    18301 ataacagcca tcctcctgaa aaatctggcg tgccttcagc cgttaacgcc atcactgcta
    18361 agattccttg tggtgtaggt agatccgata cagcatccat gacctcatcc gaaactagaa
    18421 aagtagaaat ctctttgttt gcggccagcc agtctcccca ttctttcatg cctcgctgtg
    18481 tcacaaaaag ttcttttatt tctgcatcag acgacgcagc ttcttcaatc agatggaacc
    18541 cttccaaaag atattcttcc ttttcttccc gatatttctt tttatgtagt ttttttaatt
    18601 ctttgattct tggatttttt gctgattgga tctctttcac attcctcacc tccccatgat
    18661 tatatcatgg aataggtgct tgagcttgtt tcatcctaaa aaaatcggta tcattaattt
    18721 ataaaatagg aaagaaaggt gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc
    18781 gtccaaggcg taggatttcg ctacatgacc aagatggtcg ctgatcaact aggcgtgact
    18841 ggaacggtaa aaaacgaaga tgacggctca gtcacgatcg aagcaatcgg caatgacgac
    18901 atcatccaga aatttattga agaagtcaag aaatctccga gcccaagtgg acgagtgcag
    18961 tatgtggatg tccaagaaga cccgatgatt gaggaacgaa agaaatttga tgtggtggga
    19021 taaggttgtg aaaaagctgg tctgcatcaa gcattaagga caggttgtga aaaatagctc
    19081 tccatatttt tcaacaggct ggacttaatg ccgtagatgc aagcttttga acaccgtcaa
    19141 taaggttgtg agaaagtcgc ttagttttga gtagtaagga gaattttgtg aaaatggctt
    19201 tccacatttt tgcataaaat ttgacttaat accgagaaac tgacttttga acaccgtcga
    19261 taaggttgtt tttgagacgc tttgcctcaa gtattaaaga taggtagtaa aaaagctgat
    19321 ctataacttg caataaat
//
LOCUS       DARBDV010000057        19200 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000057 DARBDV010000000
VERSION     DARBDV010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.057"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            102..2543
                     /locus_tag="SCM56_001845"
     CDS             102..2543
                     /locus_tag="SCM56_001845"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEG1110610.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2873..3577)
                     /locus_tag="SCM56_001846"
     CDS             complement(2873..3577)
                     /locus_tag="SCM56_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110611.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3764..4009)
                     /locus_tag="SCM56_001847"
     CDS             complement(3764..4009)
                     /locus_tag="SCM56_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110612.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4026..4877)
                     /locus_tag="SCM56_001848"
     CDS             complement(4026..4877)
                     /locus_tag="SCM56_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEG1110613.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5043..6047
                     /locus_tag="SCM56_001849"
     CDS             5043..6047
                     /locus_tag="SCM56_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEG1110614.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(6288..6644)
                     /locus_tag="SCM56_001850"
     CDS             complement(6288..6644)
                     /locus_tag="SCM56_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEG1110615.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            7012..8199
                     /gene="tuf"
                     /locus_tag="SCM56_001851"
     CDS             7012..8199
                     /gene="tuf"
                     /locus_tag="SCM56_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEG1110616.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(8387..10978)
                     /gene="mprF"
                     /locus_tag="SCM56_001852"
     CDS             complement(8387..10978)
                     /gene="mprF"
                     /locus_tag="SCM56_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEG1110617.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11004..11765)
                     /locus_tag="SCM56_001853"
     CDS             complement(11004..11765)
                     /locus_tag="SCM56_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG1110618.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            12043..12564
                     /locus_tag="SCM56_001854"
     CDS             12043..12564
                     /locus_tag="SCM56_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HEG1110619.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            12623..13483
                     /locus_tag="SCM56_001855"
     CDS             12623..13483
                     /locus_tag="SCM56_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEG1110620.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            13668..15983
                     /locus_tag="SCM56_001856"
     CDS             13668..15983
                     /locus_tag="SCM56_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEG1110621.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            16165..18006
                     /locus_tag="SCM56_001857"
     CDS             16165..18006
                     /locus_tag="SCM56_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEG1110622.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            18030..19139
                     /gene="rpoD"
                     /locus_tag="SCM56_001858"
     CDS             18030..19139
                     /gene="rpoD"
                     /locus_tag="SCM56_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEG1110623.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
BASE COUNT         6226 a         3392 c         4028 g         5554 t
ORIGIN      
        1 tttaaggatt gttaaaattt aatcttagaa aaaaataact ggaacaagag ctatttgttt
       61 ttacagcaaa gaatggtata tttttgtagg aggtggctta cttgaaagaa gtcgttatga
      121 tagatgctgc aagaacgccg ataggaaagt atcgcggaag tttaagtcca tttactgcag
      181 ttgaattagg cacacttgtg actaaaggat tattagataa aacaaaactg aaaaaagata
      241 aaatcgatca agtaatattt ggaaatgtcc tgcaagctgg taatggtcaa aatgttgcca
      301 gacagattgc tttgaacagc ggtctccctg tagatgtccc agcaatgacg atcaatgaag
      361 tttgcggatc tggtatgaaa gcagtgatct tagcacgcca gctgatccaa ttaggtgaag
      421 cagaacttgt gatagccggc ggaacagaga gtatgtctca agcacctatg ctgaaacagt
      481 atcagtcaga aacaaatgaa tatggtgaac caatttccag tatggtcaac gacggattga
      541 ctgacgcatt ttcaaatgca catatgggat taaccgcaga gaatgttgca acacaatttt
      601 ctgtgagcag agaagagcag gatcgctatg ccttgtcgtc ccagttgaaa gcagcacatg
      661 ctgtcgaagc cggtgtattt tctgaggaga tcatcccagt caagatttct gatgaagacg
      721 tgttatctga ggatgaagca gttcgtggaa atagtacatt ggaaaaactg ggcacgttac
      781 gtacagtatt ctcagaagaa ggaactgtaa cagcaggaaa tgcttccccg ttgaatgacg
      841 gtgcctctgt ggtgatcctt gcatccaaag aatacgcaga aaataataat ctgccttatt
      901 tagcaaccat caaagaagta gcggaggtcg gtattgaccc aagtatcatg ggaatcgctc
      961 caatcaaagc cattcaaaaa ttgacagata gatcaggaat gaatttatca acaatcgatc
     1021 tttttgagat caatgaagca tttgcagctt cttctatcgt agttagccaa gaactgcagt
     1081 tagatgaaga aaaggtcaat atatatggag gagcaatcgc attaggccat ccaattggtg
     1141 caagcggtgc acgtatcttg actacattag cttacggatt gcttcgtgaa cagaagagat
     1201 acggtattgc ttcattatgt attggtggag gtttaggctt ggcagtcttg ttagaagcga
     1261 atatggagca aacacataag gatgtgcaaa aaaaaaagtt ttatcagctt acgccatctg
     1321 aacggcgcag tcaattaata gaaaaaaatg tattgacaca agaaactgcg ttgatttttc
     1381 aggaacaaac actttctgaa gagctctccg atcacatgat cgaaaaccaa gtgagtgaag
     1441 tggaaatccc aatggggatc gcgcagaatt ttcaaatcaa tggcaagaaa aaatggattc
     1501 cgatggcaac agaggaacca tctgtcattg cagctgcaag taatggtgca aaaatctgtg
     1561 gaaatatctg cgcagaaaca cctcagcggc tgatgcgagg gcaaatcgtt ctatcaggaa
     1621 aatcggagta tcaagcagtg atcaatgcag ttaatcatcg aaaagaagag ctgattcttt
     1681 gtgcaaatga aagttatcct tcaatcgtga aacgcggggg cggtgttcaa gatatctcta
     1741 ctcgggaatt catggggagt ttccatgcat acttatctat tgatttttta gtggatgtaa
     1801 aagatgcaat gggcgctaac atgatcaact cgattcttga gagtgtggca aacaagttac
     1861 gggagtggtt ccctgaagaa gagattctat tcagtatttt aagtaatttt gctacagaat
     1921 cattggcttc tgcctgttgt gaaatcccat tcgaacgctt agggagaaac aaagaaatcg
     1981 gtgaacaaat tgccaaaaaa atccaacagg caggggaata tgcgaagctt gacccttacc
     2041 gtgcagcaac gcacaacaaa gggatcatga atggtataga agctgtagtg gcagctacag
     2101 gaaatgatac acgtgcggtt agcgcttcga tccacgccta cgcggcaaga aatggattat
     2161 accaaggatt aacagactgg cagatcaaag gggataaact agttggaaaa ctaacggtgc
     2221 ctttggctgt tgccacagta ggcggagctt ccaatatcct gccaaaagca aaagcatctt
     2281 tagctatgtt agatattgat tcagccaagg aactagcaca agtaattgct gcagtagggt
     2341 tagctcaaaa cctggctgct ttgcgtgcat tagttacaga aggaatccaa aaaggccaca
     2401 tgggacttca agctcgttca ttagcgatct caattggggc tattggtgag gaaatcgagc
     2461 aagttgcgaa aaaattacgt gaagcagaaa agatgaacca gcagacagct atacaaatat
     2521 tggaaaaaat ccgggaaaaa taaaaaagca atatctttag aagaataaag caagatttac
     2581 agttatttta tttactgcag atcttgcttt tttctctatc agaaaatatg tttattatta
     2641 gaatttttta tagttaaggt taggcatctt aagaaatcag gcaactccaa gatatcttca
     2701 ggtagaagag aagtcgccta acattgtagg atcataggtg atttgttgga caggtcaagc
     2761 tgatatttga gcctgatgtt agaaggaatt tctttttact tgctgtagaa attattatat
     2821 gactaaaaag gctgggattt taaatcccag ccttttatga caaatagaat ttttatcgat
     2881 cagtaatcgc tgtcgctgca aattgttcga tcaaaggaat caaagtcaat ttgactttgt
     2941 atttccgttc gagtgctttt tcgataacga actcattttc agtagtcata gaaatagttg
     3001 ctaatttacg aggttcatca gcattttttt cattaataac tacttcatgt tcattttgtt
     3061 ctgtgtaaaa tttaggatac tcagcaacgg cttttgaaaa atcagcaaaa cttaaagcgg
     3121 cattcgtatc agttgagcgt gtattttcag ttgtgtccgc ttttggtttt tccgtttttt
     3181 cttctggcac actgactgtt tcttctgtaa ctggtgtcgc aacagcttgc gcagttggtt
     3241 catcgttttt caatcgttga tcattccagt aagacacgat ttcaataaga agattgtcac
     3301 ggaatcgttc gaaaatcccg ttttctcctg aacgaattct ctttaataca gtattgaagt
     3361 tgtcatcgat tggcacgttg atccgtgtat cttcatttgt tttcccagct aatccgatcg
     3421 ttaagatttg acggtttttg ggatcaagca tgacttgtaa cggcaaacca taacgtcgca
     3481 agctctcttc ttctagagaa cggaataatt gtcctcttac accatccaat gaaggacgga
     3541 attgatttaa taatttttgt ttctgttttt tcttcatatg ttctctcctt cgctcatacc
     3601 ctttcgatta taagaaaaaa acagccaaat gaaaaggagt aatgcaaaaa aacttgcgta
     3661 tacgcaaatt acaacagaaa aatttgcgta ttttcccgtt ttttgaaaga aaaataaaaa
     3721 atggaacgga ttatgtatat atacataatc cgttccattc tttctatttt ttcggtatca
     3781 aggcaaaaac aggtagttca ggtgaataaa tgacttcatg ttcgtgccag ggtttgacca
     3841 ttagcgaatg atcgaaccgt tttttcagtt cttcgatgat ttgatcatgt gtccagtttc
     3901 ttgcaggaaa atgttggatt ttctctgcat cgatcaaaaa gacaaaatcg gaatgaaaag
     3961 tctgggtaac ttcttccgag agagaattca agcgttcgat tggatccata aaatcacctt
     4021 tcgacttaaa attgggtagt atctgggtct tttgctttgc cgactacacc atctaactga
     4081 tcgatttgtt tcatttcttc ttctgttaat tcaaaatcaa ataattcagt attttctttg
     4141 attctgcttg gtgtcactga cttaggaaga ggaaggaatt cgtgttgcag cgaccaacgc
     4201 aaagcaactt gtgcaatcgt cttgtcatgt gcttcagcga tttgtttcat ttcaggcaca
     4261 tcgaagattt taccagtgcc taatggacta tatgcttcga ggatcatatc atgttttttt
     4321 gcatactcaa ctacagttgt ctgtaattcg cctggtgcaa ggaaaacttg attgaccatc
     4381 ggcataattt ttgctgtttt tgctagtgta tctagatggt ggggtaagaa attgctcaca
     4441 ccgattgctt tgattttacc tgcttcatac agttcttcaa atgctcgcca tgtatctgca
     4501 tttgcttgtt cccaattatc acgaaaagca acagggtttg gccagtggat caaaaacaga
     4561 tctaaataat ctgtttggag cttcttcaat gattcttcaa aagaagacat cacaagttca
     4621 tagctgtggt ttgcattcca taattttgtt gtcaagaata tctcttctct aggaattcca
     4681 ctttccttaa tcgcttggcc gacgctttct tcattcttgt atccttgggc agtatcgata
     4741 tggcgataac ctgcagccag cgcttcttta acagaggaaa cagcgacatc cccatcaggt
     4801 gtttgccatg tgccgaatcc tactaccggg atttcgtaac catttgatag acgataagtg
     4861 cttgttaatg aattcatact ttcagctcct tttttattcg ttcaactata agtgtagcgc
     4921 atttttcgaa aaaaagaatc cattttgttt ctactcttga aatttttgtt taaaagaaaa
     4981 tgattttgat tctggtattt ttgatttttt ttccgtaaac taagacagaa gggaatgaga
     5041 aaatggatat ctatttaaaa aaagcaattc tgcatattat tgacagagaa actggttctc
     5101 cagtcttttc acaaaaagag ctggatctga caaaagaata tattcgtgat tttcttcaaa
     5161 agaagatcca aaaaatctct tctgctcaaa caaaaactgg acaactagca gaagaaagca
     5221 tgatcgttga atcacttaaa atcgctcaag aaaattttgt ggaagcaagt gaagcaatcg
     5281 ttcagcggtg gttcgatatc taccaagaaa gtgaagaagc acctagtgca gatgtatttg
     5341 ttgtcttgta tgaattagat acagtgatgt acttatcttt attgaaagtc aattatcgtg
     5401 aggcttacac tcactttgtt gaagcagacg aagcagggat cgacaaccgc ttgattttga
     5461 atcgagcaat tctaggggcg aaaacacaaa aagcagatga agcattcgct gtgaatttga
     5521 ctgattggac ctacgaatta attgaaaaaa aatatgagtt ttctggcaaa aaagagcttt
     5581 atttttctgg aagagtgatc gaaagtgttc ctgcaccttc tttagaagaa aatgtaaaag
     5641 tgatccaaaa agtagccaag catcttggaa aaaaatttga gacagaagaa tttgatatca
     5701 tggctgattt gaaagaagct gtctatgata cgatagaaga aaaaggtcgg ttggatcatg
     5761 agatgattgc ggaaaaagtc ttcaaggaaa acatcacggc aaaaatggct tttaaagaag
     5821 aagtacaaga gcaaggattt gtcccagaag cacctcctgt aaaagaagta caagaaattt
     5881 ctgagaaaaa atttggaaaa caaaaattca agctatcaaa cgggatcgaa ctgatcgtac
     5941 cagtagatgt atatcgcaac ccagatttga ttgaatttgt gaataatccg gatggtacga
     6001 tatccgtgat gatcaaaaat gtggatgaag tattgaatcg tttataaaaa tgactagaat
     6061 attaggtgtt caagtaccag tgatcagtta aatgaattgt gtcaagagaa ggatcggaaa
     6121 agacgaatat aacaaaaaaa gagcgtgaga caaaagtaaa gaacactttt gtctcacgct
     6181 ctaaacacgt ataaacggcg gaagcagaag caactccttc ttatttcttg gagttaaacg
     6241 cttctgtccc agcctctttt tatctaataa aattttaaga ccatttacta attatcgatt
     6301 ttttctgccg catttttagg cacttcagaa gcctttattt cttcccagct taatacacct
     6361 ttacgaggat tgactttgag tttaagatac gcattcatct ttaatggatg ttctctttct
     6421 tcgcgaagcg tcacctgttt tttttcacca tctttattat aagccgcttg cttgtaggta
     6481 taagtcgtga atttctctcc gttatctgct tgggtagctg acatttcgcc attcgtcgtg
     6541 atttttgtat aataagcttc gccgccgtaa aaataatgat aagctgcaaa acttccgccg
     6601 agaaatatgc ctaaaacgac aagtacgcca attaattttt tcatgtactt cacctctttc
     6661 cttattatta acgaaataga agatttcgtc tattgaaagt tgttggcaac agctgaattt
     6721 taaacttttc ctcattttgt tcttattgct tcaaaagaaa gttaaggact atcttgaagt
     6781 ttttctcaaa aaacacattt ttttatttat aaaaggtact tttttcgcgt ataagacaga
     6841 cgagtgctct tagttgactt tgaccaatat tgacgtataa taagcttagt tcgaatctta
     6901 caaaaagtaa gtcggacgta ttctttttga tttagtcaaa aaaagactat aataaaaagg
     6961 tgagagctga aatttttcag tgatctatat tatttaggag gtttgtaaat tatggcaaaa
     7021 gaacattatg acagaagtaa accacacgtt aacattggta cgattggaca cgttgaccat
     7081 gggaaaacta ctttaactgc tgcgatcact acagtattag gcaaaaaagg cctagcaaat
     7141 cctcaagact atgctagtat tgatgcagct ccagaagaac gtgaacgtgg gattacgatc
     7201 aataccgcac acgtagaata tgaaacagaa aaacgccact acgcgcatat tgatgcacca
     7261 ggacacgcgg actatgtgaa aaacatgatt acaggggcag cacaaatgga tggtgcaatc
     7321 ttagttgttt ctgcaactga cggtccgatg cctcaaacac gtgaacacat tttattgtca
     7381 cgccaagttg gtgtaaaata cctgattgtt ttcttgaaca aagttgattt agtcgatgat
     7441 gaagaattga tcgatttggt agaaatggaa gttcgcgagt tattgagcga atatggtttc
     7501 ccaggcgatg acactcctgt gatcaaaggt tccgcattaa aagcattgca aggcgatcca
     7561 gatgctgaag ctgctattat ggaattgatg gatacagtag atgaatatat cccaacacca
     7621 gaacgtgata cagataaacc attactattg ccagtggaag acgtcttctc aattacaggt
     7681 cgaggaactg ttgcctcagg tcgtattgat cgtggtgctg ttcgtgtcgg tgatgaggta
     7741 gagatcgtag ggatcaaacc tgaaacacaa aaagcagttg taacaggtgt agaaatgttc
     7801 cgtaaaacgt tagattacgg ggaagctggg gataacgtag gcgtgttgtt acgggggatc
     7861 caacgtgacg atatcgaacg tggacaagta cttgctaaac caggttccat tactccacat
     7921 acaaaattca aagcagaagt gtacgtgttg acaaaagaag aaggtggacg tcatactcca
     7981 ttcttcaaca actatcgtcc acagttctac ttccgcacaa ctgatgttac aggaacaatc
     8041 acattgccag aagatacaga aatggtcatg cctggtgaca acgtgacgat tgatgttgac
     8101 ttgatccatc caatcgctgt tgaaaacgga acgacattct caattcgtga aggcggacgg
     8161 acagtaggtt caggtatcgt tacagagatc gaagcataag aaacgcgact cgaatatttt
     8221 ggtaatgaaa aatgtttaaa ataaaagagc ggtccaaaca ggatttctgt ttggaccgct
     8281 ctttgttctt caattgtata agaagagatt gtagaagcat gaactttcgt tttacttcag
     8341 tctcttttat tcatcatgag agtgttcatc tatcggtatg cagtttttaa tactttcttc
     8401 gtatccattt tttcaaacca cttattctac cttgtgtctg ctcaactggc gcattatcaa
     8461 taatcagcaa agcaatcata acatatgcaa tccaactatc tctagaatat agagtataac
     8521 gtgagaccca gtttgtagcg tacttgcttt tatattctct taatccatgg aaagaataaa
     8581 aatgagaacc aaactcgtaa accaagtatg caatcctttc ctgtaaaaaa ctctttctgg
     8641 aagttccgac atttgaaaga ggggccatcc ctaaattgaa ccattggata tgttcttctt
     8701 tcatataggc aaacaaatgc aagaacaaga aatccatact gccagaaggt gcttttttag
     8761 aatcatatcg catcagatca atcgttccta ctttattgtt cgtgtaagta ggcatgatcg
     8821 ttgcaaaagc gacaatttct tcttgggtat ttttgacaac tgcaataggc gctctttgta
     8881 gataatcttc agagaaaaag cctaaagaaa accctttttc ctttcgtgaa cccaaccaat
     8941 tatcagagat atttttgaaa gtactcattt gctcagtaga aaatggtggt tgtaagacat
     9001 caaatgtaaa tccttctcgt tcgatccgat tcaagacagc acgagtgccc ttcatttttt
     9061 tccctgaagt tgtaaaacta ttcaggtcta cataagcttc ttcccccatc ttaatgaaat
     9121 cgtagccgaa ttcatgcaaa atcataacaa tctcttcgct tgtttcgtag aaaactggta
     9181 aatagcatag tcgatctgtt tcttcgataa aagcttcgat tgcttctgga aaatcttctt
     9241 tcttaccaga aggatcaccc attacaatac atttattatt gaagcaagcg aattggagaa
     9301 gaacagttgg ttcaccgtct ttttcatagg caaacatccg cttatctttt aagaagatca
     9361 attgactatc ggagtttcct ccataagttg taaggatctt cagggccttt tcttcattaa
     9421 aggcttcgcc aatctgtttc ttttctcctt gaaggaaatg aacaaataga acgatcatga
     9481 aactaacagc gatgatggcc aacaatcccg aaaaccaaat cttttcagaa ggaaataaga
     9541 aaaaggaaat aaaatggtga cgccgatgag ggaaatccgg cagattgtat accccaataa
     9601 cgatgtaaag caaggacagt gcaccaataa tgattccatc gatcgtacgc cactcccatg
     9661 aatagatcaa ttgttcccga aataattcgt ttttagaagc aagaatgatc agaagcagaa
     9721 tagacaagaa aaccaccggt gtgaaactaa aatcacttaa taaaacataa agtaaagcta
     9781 gaacaatcaa gaagattgta ggcaaatatg ctcgtttcac acgtgctgcg atccctctgc
     9841 ccataatgat taaaagaaat cccaatagta tactaggaaa ttggataata aaatgaaatt
     9901 tcaatgggtt caaactatga agccagcgaa actctgtaaa agcttgcggg atagtggcag
     9961 aaagtactag catgattcca gaaaagtata ataagacaac cacgatttta tgcgcaattt
    10021 ctgttgctaa ttgtttaggg ataccagaat aacgctgatc aaaagtggaa ccgatatttt
    10081 tgaagaaaaa gacgattcca atcaaaaacg gaatgatgta gtagaacagg cgatagagca
    10141 agatccacac aacgactatt tctcttggca caccaatagc ggacaacccg ataatcatca
    10201 ttacatcgaa actcccaatt tctcctggaa tcatagacac gattcctatc actgaagcag
    10261 caatgaataa tggaattgtt tgccacagat caatccggat ttccattaaa taaccaacag
    10321 aaaggaaact aaacaggaca ccggtccatt ccaacaatga aactagcaat agctgtcctt
    10381 gagttttaag tgaggtacca ccaaggaatc ctttcttttt gaaagtagta aataaaaaga
    10441 cgacaggaaa atatagaccg ccgccgatca accatatcca atattgcttc aaaaaaggag
    10501 caactggcgt gaagactaca agtaaaaaag aaagcagact gtaaatagaa agtccagcca
    10561 tcaaaaacaa gaaaactttc gataatgctt ggatcacgtt tctgctctct ttcttttgtc
    10621 catatagttc agagcgaagc cccacactaa caaacccgcc gaaaccggca atattgttga
    10681 tcgtattgat cagccagctt gtctcaaaca ggtaacgagg attctgcttt tgttcaagca
    10741 tccgattcaa aataatatca taaccaacca taggtaacac agagataaga ccaatcaata
    10801 gcattagtcc gcttttccat acaggtatag tggcaaacgt ttctcctaat tgctggatag
    10861 atagagtttt accgatggac atcaattcac cgacaataat gacaatgaca gaaatcaaaa
    10921 agattgtttt gaacagtcct aggtggtttt tcagccattg tatggtattt tttaacaaat
    10981 aaatcacctt ctattcctaa tagttatgtt tcagaaagaa attgaagaac ttctcgattg
    11041 aagcgttcag gatcttttct agctaattca tgtccttgtt ctttcacaag cacaaaggaa
    11101 gcttttggta tagttttagc tatatacaaa gaatgtttta gcttgattac atcttttttt
    11161 cccacgatga tcaaagtggg ggagttgatt ttttttaaat cattttctgt aagcccgatg
    11221 tcatgcagca aaagtttgat aactaaaaga tttttgcgaa gacttgggcg aaataaagag
    11281 agtaaccata cccatgcata atgaatatta ctgatgactc tggcagaaaa acgaacgcct
    11341 ttaacaagcg tgtttccaga atttaaaatc aatcgatgaa cttttttagg gaagctgctg
    11401 gcaaatacca gtgctagatt tgctccatca ctaaatccaa gaaaatctgc ctgatcgatt
    11461 ttttccagca acatgatcgt attcagatcc tcggccatta actgaaaatt caacatagat
    11521 gcttcgtttg tcgaacgacc atgtcctcgg ctatctacta gatacacggt atagtagcgt
    11581 tcgagtacag ggacctgttc tgaaaaaaag cggccgctgc cgtcattgcc atgcagtaaa
    11641 aagagaggga agccttgacc gctcttttca tagtaaattt cgctcccatc tggcattttt
    11701 gcatatttct tttctttttt cacataatca cctactgtgt aaagtataac gattatatca
    11761 gtcattgggc aattgttttt actgaaaaac aacgatcatt ttcgtaagat gtcaaaaaat
    11821 acaggaaaat tcattttttc ttaaggcgta cacagaaatc aaggaagaaa tagccaagaa
    11881 gataaagaaa tagtaagata aaaaaagatg tcaaaataac taggtgatga aatgaagcga
    11941 aaacgattat ctattatccg tattcttttt tacatgatca ttctgacggt tattggtgcg
    12001 gtgttgtttt tatgaggaag aacttatcgt tcggtaaaac gagtggaaag ctatcaaata
    12061 gaaataacaa cagctgcaaa aaattatcat atggaaaact acgagaattt gatcaaggcg
    12121 ataatattga ccgaatcaaa aggaaaaggg acagatctca tgcagagctc ggaaagtctg
    12181 catggagaag ctgagatcat tgataatcca gctgacagta ttgatcaagg tgtcaaatat
    12241 ttggcggaaa tgatagtaga agcaaagaaa caaggatgcg atttatggac agctgtccaa
    12301 gcatataatt ttgggaaaga ttatattgcc tatgtaaaaa atcatggcgg agaaaacaca
    12361 gtagaattag cagaagcgta ttctaaagat gtgctgagcc cacttttagg aaatgaggaa
    12421 gggaaacaat accgatattg gcggatccag tccttgcttt acaatggcgg ctatctctat
    12481 cataatggag gtaacatgtt ttatcctgat attgtcaaaa tgaatgaaaa aataatagag
    12541 acctatgaaa aagtctttaa gtaaaatagc aggaaatgtt caatcatgaa taaaattaaa
    12601 tgaaagaagg agtgcgcaaa tagtggaaaa aaggcaaaaa atggaaaatc aagaagtggg
    12661 gtacacaacc aaagactgga tgttacggtt tgtgaaggga atgtttatcg gttcaggatt
    12721 tatcttgcca ggagtcagcg gtggtgcatt agcagctgtc tttggcatgt atgagcggct
    12781 gatttctttt ttagcccacc ttacaaaaaa tttcaaagaa aatgttttgt tttttctgcc
    12841 tgttggtcta ggcggagtga caggcatctt tctactttct tttttagtca gttttttatt
    12901 aggtgcgtat gaaacaacga tattatggtt ttttgtcgga tgtatcgctg gaactgttcc
    12961 tgcgttatgg aaagaagcag ggaaaaaagg aagagatact tctgatgtga tcatcatggt
    13021 tgttactttt gttctggctt atttgttttt attatacggc gctcaattat ttgacgggca
    13081 agtcgatcag aattttggaa catggctgat tgccggcgga ttgattggtt tgggtatgat
    13141 cgtcccagga ttgagccctt ctaatttttt ggtgtatatg ggattgtata aagcgatggc
    13201 tgacggattt aaagaagtac gacttgaagt gattgtgccg atcggaattg gtgggattct
    13261 ttgtgttctt ggattgtcaa aaatcatgga ttacattttc tcaaaggcat atagcaagct
    13321 attccatttc attttaggtg tagtttttgc ttcaacgatt atgatcattc ctaccgatta
    13381 cgcgggattg ggagttgctg gggtactagc ttgtcctgtt ctttttatag ccggtacagc
    13441 actaggttgg tggatgagcc gtttggagga acaatataag taaagataga ttgataataa
    13501 aaacagcaca cgggtgattc aaacaaaaag gttggcatct tttttgtaaa atcattcgtg
    13561 tgtttttcta taaattttat aaaaacaagt tgtttttaat cttggaacac gctagactta
    13621 aacacgtgat tatttttgaa agttggcgga aaatggagaa aaagaaaatg aatggactta
    13681 cacagcaaga ggtagttcag aaaatggctt ctggccagca aaatgactac caggaagatg
    13741 catcaaaaag tacaaaacaa atattcagcg ataatatatt gacgttattc aattttctga
    13801 actttggtat tgggatttgt ctgttatttg taggtgcgta ttccaacatg gcgtatttag
    13861 caattattgt cgtcaatatc gttattggaa tttatcaaga aatacatgca agaaatcttg
    13921 tacggaaatt atcgattgtt tcccaatcag aagtactagt gatccgggac ggtaaagaac
    13981 aatcgatttc tactaaagag ctggtattag gagatttggt aaaaatcgga gcaggagaac
    14041 agataccagc cgatttacag gttatatctg gacgggcaga agcaaatgaa gctcttttga
    14101 caggcgaatc tgatttgatt gtcaaagaaa ctggagctga attgctttct ggaagctttt
    14161 tgacaagcgg ttctgtcatg gctgaagtta tccgagtagg tgcagataat tatgctgtcc
    14221 gcttgactag cgaggcaaag actcacaaag ctattcgttc tgaacttgta gattcgattc
    14281 gcaaagtatc caaattcact agtttcgtga ttattccttt ggggatcata ctatttttgg
    14341 aagcactgtt catccgcgat gcttctatca aaatttctgt tattgcatct gcagcagctt
    14401 tgttaggcat gcttccaaag ggtttggtat tgctaataag tattgcatta acgacagggg
    14461 taaccaaact ggcaaaaaaa cgaatccttg ttcaagacat gtattcaatt gaaacgctgg
    14521 cccatgtcga tacattatgc ttggataaaa cagggaccat tactgaaggg aaaatgagta
    14581 tccagcgggt agaagcacta catccgctgt acgagaaagc catcccagaa attatgggga
    14641 cgtatctggc agaaagcagt gataacaaca tcactatgcg ggctatgaga gattattttg
    14701 agctttctaa tcggtatgat gtgacaaaca aagtcgcatt ttcttctgat cgcaaatggg
    14761 gcgcgatgga atttccagaa ctgggagtcg tgtacgtagg agcgcctgaa cgtttggtgg
    14821 aagaagatcg gcttccggac agtctaaaaa cagcccaagc gaatggctat cgcgtactta
    14881 tgttggggat cgctccaaat cagcaattga acgaagaaaa accagcattt gttgagccgg
    14941 ttgctctgtt tgaaatcgat gatccaatcc gaaaaaacgc tcaagaaaca ctagcttatc
    15001 ttcatgagga aggtgtggat ctgaaagtga tttctggaga caatcctttt actgtctcca
    15061 atatcgctag acgtgcagga ttaccgaatt atgatgcgta tgtcgatctt tctcaaatta
    15121 ctgaagaaat ggaagtgcga gaagctgctc atcgctatac ggtttttgga cgagtgagtc
    15181 cgcagcaaaa gaaattgctg gtaaatgaat tgaaagaaag cggcagaacg gttgcaatga
    15241 caggcgacgg tgtgaatgat gttttggcgt taagagaagc agattgcagc attgctatgg
    15301 cagaaggtga tggagcaaca cgtcagattg cgaatctggt attgttggac tctgatttta
    15361 ctacattgcc agaagtcctg tttgaaggac gccgagtggt caacaatgtg acgaaagtct
    15421 caggcatctt ttttatcaaa acgatttatt catttatttt atcgattata tgtgccgtta
    15481 cagcaattgg atttccgttt atccctatcc aaatcacgct gattgattta gcgattgaag
    15541 ggtatccttc cttcttttta tcttttgaac aagataaaag aaaaatcact taccgttatt
    15601 tgcctacagc acttattaat gctttaccaa atgccttgct tgtagtagtg aatatcattg
    15661 cggtgtatct acttggaaaa acattcgcat ggggaaatgc tgaaactact actttgatgt
    15721 attacttgct gattgggatc agttgcatgg cagttgtaaa agcttgttat cccttcaacc
    15781 cattgcgcat cttcctagca gtaacgacga tcgtagggat atatgtagct gcaatgctgt
    15841 tccatggaat tttggaagta aacttagggt tgggagagag cgtaggatac ttctttgtat
    15901 tcatgctcgt aaatattgtt cttcgagtat tgtactggaa ggctgatatt caaaatcagt
    15961 tactgaaaag agtagcaaat tgatgcttaa tgagatagaa atgtgttata cttgaaaaag
    16021 caatgaaata tgatacaatc aaacatgtat aattgtgtcc tttaaaatcg cactgatttc
    16081 agttagtgcg attttttacc ggttattctc ctgaaatatc ttctgttaaa cccaaaagat
    16141 atcaatagag cagggggtga aaaaatggct caacggattc ctcaagaggt tatcgaaaca
    16201 gtacgcagtc aaacgaatat cgttgaggtg attggacaat atgtccagtt gaaaaagtct
    16261 ggtaaaaatt acttggggct gtgtcctttt catgaagaac gtacaccgtc tttttctgtt
    16321 gcagaagaca agcagatttt tcattgcttt ggttgtggta aaggcggaaa cgtgtttact
    16381 tttcttcaag aactagaagg tctaagtttt ccagaagcag tggtaaaagt ggcagacttc
    16441 gaacagatac cgttagaaga tcaatggcgt cagcgtacta tagctgtcca aaatcctgaa
    16501 tctgctacgg ggaaactgat tgctgctcat gaaaaagcag cggaaatcta tcatcacatg
    16561 ctattgcata caaaagctgg acagccagct ttagaatatt tgcaaagtcg tgggttgtct
    16621 ttagaattaa ttgaagaatt caacataggt tttgcaccta atgagcgttc ttttcttcag
    16681 caagtattca agaatgaatc aatttcaaat gaattgttag aacgctctgg attgtttgtc
    16741 acgcatgatg atggccgtat gtctgatcgt ttttatcaaa gagtgatgtt tccacttcgt
    16801 aattctcaag gccagacgat cggtttttca ggacgctggc ttgcacctga aaaagggcag
    16861 gaaaaagatc agcccaaata cttgaatagt ccagaaacag aactgttcaa taaaagacaa
    16921 gttttattta atcttgataa agcaagaagc gatatccgga aaaacggaga aattttgttg
    16981 ttcgaagggt ttatggatgt gattgctgca tggcaggctg gtatcaaaac tggattagct
    17041 tctatgggga caagtctaac agctcaacag atccaacaaa tggaaaagct gacgaaagaa
    17101 ttggtctttt gctatgacgg agacaacgca ggttttgaag caaccaatcg agggattgaa
    17161 ttgcttcggc aaaacagtcg attagcactt tctgtcgtag tcgttccaga aaagcttgat
    17221 cctgatgaat atctccgtaa gtatggagaa gcatctttcc aagaattagt ggctcatggg
    17281 cgcgaaacag tctttacttt taaatcacgt tatttaaaac aagggaaaaa tttagataat
    17341 gaaaaagaca agatcgaata cctcgaagaa ttactagttg aacttagccg agttgtttca
    17401 ccaatcgaac aagatatgta tctatctcaa ctgtcaacag agtttcagat cggcagagag
    17461 acgatacaaa aacaactgcg cgatcttcga aggacaaatc agcaaaatcg tcaagaacct
    17521 caggaaagtc aacacactca ggttacgaga caagaaactg cacggaaaaa gagacctttg
    17581 acgcaagtag aaaaggcaga gcagattcta ttatatcgtg catttaaaga agtttctgtc
    17641 cgtaatattt taaaagaacg agaatttcag tttattcatg atgcatatgc agaagtttat
    17701 tttttattcg atgcatatgt ctcacaacat tatgaattca accttgcaga gtttttagac
    17761 tttttgcaag atgaaaattt aaggcggttg gtggtggaaa tcgaatattt accggtggca
    17821 gaagaaagtt cgccacgaga aatccaagac ttgctagatg tgatcagcaa atctggctta
    17881 gctgatgaaa taacattaaa aaaacaaatg cagcaagaag ccagacgaac tggaaacaaa
    17941 cagcttgagc ttgaattgac gattgaaatc atcaatttaa cgaaacagtt aaaacaagcc
    18001 aaatagctac tcaagggggg ccttctttta tggcaaatgg aacagataaa aaaacgtatg
    18061 aagcagcagt agcagctttc atcaaagaaa acaaacccaa acgacaagtt gtatatgatg
    18121 atttatccaa tcagcttgct acaccattca ctttgaatgc agatgaaatg gacaaattga
    18181 tccaaaagat tgaagacgca ggtatcagtg tggtggatga aaatggtgat ccatcaattc
    18241 acagcttgaa gagtgctgag aaaaaagctg aacaagcaaa aacagaagat ctttctgcgc
    18301 caaccggtgt aaaaatcaat gacccggttc gtatgtatct gaaagaaatt ggccgtgtct
    18361 cccttttaac tgcggaagaa gaagtagcgt tagcattgaa aatcgaagaa ggcgaccaag
    18421 aagcgaaaca gcgtctggca gaagcaaacc ttcgtttagt agtaagtatc gctaaacgtt
    18481 atgtaggacg aggcatgcag ttccttgact tgatccaaga aggaaacatg ggactgatga
    18541 aggcagttga aaaatttgac taccgcaaag gatttaagtt ttcaacctat gctacatggt
    18601 ggattcgtca agccatcact cgagcaatcg ctgaccaagc acgtacgatc cgtattccag
    18661 ttcatatggt tgaaacaatt aataaattga tacgtatcca acgtcaattg ctgcaagacc
    18721 ttggaagaga acctacgcca gaagaaatcg gtgcggaaat ggatcttcca acagaaaaag
    18781 tccgagaaat cttgaaaatc gcgcaagaac ctgtttccct tgaaacgcca atcggtgaag
    18841 aagatgactc tcacttaggt gatttcatag aagaccagga agcaacaagt ccagcagaac
    18901 acgcagctta tgagttacta aaagaacaat tggaagatgt actagataca ttgactgatc
    18961 gggaagagaa tgtccttcgc ttacgttttg gtttagatga tggccggacg agaacattgg
    19021 aagaagtagg gaaagtattt ggtgttactc gggaacgtat tcgtcagatc gaggctaaag
    19081 cattaagaaa attgcgccat ccttctcgtt caaaacaatt aaaagatttc ttagaataag
    19141 cgtggactat tgaccagaag agaaatcagt aaaactgttt ggtttatttt aaaagaaaaa
//
LOCUS       DARBDV010000058        19197 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000058 DARBDV010000000
VERSION     DARBDV010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.058"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            117..1544
                     /locus_tag="SCM56_001859"
     CDS             117..1544
                     /locus_tag="SCM56_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1110624.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1636..2001)
                     /locus_tag="SCM56_001860"
     CDS             complement(1636..2001)
                     /locus_tag="SCM56_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEG1110625.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(1998..2363)
                     /locus_tag="SCM56_001861"
     CDS             complement(1998..2363)
                     /locus_tag="SCM56_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEG1110626.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2555..2803)
                     /locus_tag="SCM56_001862"
     CDS             complement(2555..2803)
                     /locus_tag="SCM56_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110627.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2802..2930
                     /locus_tag="SCM56_001863"
     CDS             2802..2930
                     /locus_tag="SCM56_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HEG1110628.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2987..4681
                     /locus_tag="SCM56_001864"
     CDS             2987..4681
                     /locus_tag="SCM56_001864"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HEG1110629.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4685..4804
                     /locus_tag="SCM56_001865"
                     /pseudo
     CDS             4685..4804
                     /locus_tag="SCM56_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            5332..6465
                     /locus_tag="SCM56_001866"
     CDS             5332..6465
                     /locus_tag="SCM56_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEG1110630.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6496..6816
                     /locus_tag="SCM56_001867"
     CDS             6496..6816
                     /locus_tag="SCM56_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HEG1110631.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6845..7309)
                     /locus_tag="SCM56_001868"
     CDS             complement(6845..7309)
                     /locus_tag="SCM56_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1110632.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7441..8694)
                     /locus_tag="SCM56_001869"
     CDS             complement(7441..8694)
                     /locus_tag="SCM56_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1110633.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8723..9040)
                     /locus_tag="SCM56_001870"
     CDS             complement(8723..9040)
                     /locus_tag="SCM56_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEG1110634.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(9066..9386)
                     /locus_tag="SCM56_001871"
     CDS             complement(9066..9386)
                     /locus_tag="SCM56_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1110635.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9850..11265)
                     /locus_tag="SCM56_001872"
     CDS             complement(9850..11265)
                     /locus_tag="SCM56_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG1110636.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11282..12598)
                     /locus_tag="SCM56_001873"
     CDS             complement(11282..12598)
                     /locus_tag="SCM56_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1110637.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12748..13446)
                     /locus_tag="SCM56_001874"
     CDS             complement(12748..13446)
                     /locus_tag="SCM56_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEG1110638.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13735..14691)
                     /locus_tag="SCM56_001875"
     CDS             complement(13735..14691)
                     /locus_tag="SCM56_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEG1110639.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14804..15637)
                     /gene="msrA"
                     /locus_tag="SCM56_001876"
     CDS             complement(14804..15637)
                     /gene="msrA"
                     /locus_tag="SCM56_001876"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEG1110640.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15812..17446)
                     /locus_tag="SCM56_001877"
     CDS             complement(15812..17446)
                     /locus_tag="SCM56_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110641.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(17433..19121)
                     /locus_tag="SCM56_001878"
     CDS             complement(17433..19121)
                     /locus_tag="SCM56_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110642.1"
                     /translation="MKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLSRI
                     KGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLLNI
                     CTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYY
                     AYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFV
                     VVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDI
                     TTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLL
                     QFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVI
                     QSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKA
                     ADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIE
                     ITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
BASE COUNT         6094 a         3754 c         3192 g         6157 t
ORIGIN      
        1 aacctttttt aacggcattt atatgctttt tcagaaatta actgatgatt tcaaaaagaa
       61 aacatgtcat aatatttccc atattgtgca aaaaaataca aggcggaggc tctactatga
      121 ttgaagaata tattgagaaa aatattttaa gacagctgtt tttatgtggg caattctatg
      181 taaataaaga agtcaatcta gaaaagctct ccaatctcct gcatgtttgc aaaaccactt
      241 tactcaatga tatcaataac ataaaaaaag aatttgaaga acagatcgct tatacacatc
      301 gggaaaagga ccgttacact ttatattttt cagaacatat acctcgatgc aagatcatgc
      361 agcaattgtc tcaaaattcg ttgtttttaa aaacttgtct gctttatttg gaagaagatg
      421 aacctgatta tcttcaatta actgaatgtg aattcatctc tgtcagcaaa gcttattcat
      481 taaaaaaaca agtattagct tactttaatg actgcgggat cgaaatcgac cgctactccc
      541 ctcgattcac tgaaatggaa agacgtctgc tgcttcttaa tgtgtcgtat cgtttaggcg
      601 gttttaattc ttgggagcta cctgaatcgt tttttgaacg tgccgatcgt ttcattgaat
      661 ccgtaacgga aaatagtggc agattttatg ataaagaaaa taaagaaatc ttatcgattg
      721 gttttgctat cagtttccta cgccagcaag tgtgtgctgt gacaatagat tcaaaattta
      781 tcgaagaaat caaaaaacgt cctatttaca attatgtcga atcagcatgg gaaaacactg
      841 atttccaaac ctattataaa aaagaagaat tcgcctttat tttgacacta ttcaatcttt
      901 gtaattatgg tttccattct tatcagttga ttgcggaaga ctttcaacag ctacaccaag
      961 tgtttattga caatactccc gaaataaaag aactagttgc tacctttgaa tctcacttca
     1021 atcaagaatt attcggaaac caaccgttcg aacgtgcgct gattcatctg atgcgttcgg
     1081 cttgggataa ctatcaatta ttcatgccag aaaaatttta tcttttaaat gaggaacaga
     1141 caaatttata taaagaagtc cagacaattt tttccagctg gtccagccag ttaccttatg
     1201 atctacggtt gaatcctaat tgcatgcggg cttttgtgat cgaattgagc ggaattctcc
     1261 gtctgacaaa agaacatctg actatctata tcgtcacaaa ttcggatgtt cattatctga
     1321 tttatcggga agccttggaa gctgtaacta ctttcgattt tcaagttgca ccaacaattt
     1381 attcaagtat atctgatata aagaagtatg cgcagcagtc ctcaaaccgg gtattatgtg
     1441 aacgtacatt atatacacca gatgcggtcc aatatgaaaa tattattcct atttcgatca
     1501 atacgatcga tcgagctatc atttcagctg tgcaaaacaa ataaggggct gtgacaaaaa
     1561 tcatgtcaca gcctcttatt tgttgaagat gcccatttct gtaacaatct cttgatacac
     1621 gtcaggctca caacctcact tattttggac atctaggggt ccgatctctt tatttaaaaa
     1681 ataagaaatg atggttcgga taactaccaa cgccgctagt tggaaaatat cctgcaaggt
     1741 aggtttgata atcgattcga tgatcccagc tgaaacaaga atttccaaac ttagaagtat
     1801 atagcctccc aacatctttt taatagcatt gttctcgctt actcggtcgc tttgcacttt
     1861 tgtatggaca aataaatatt tcaaaaaatc cttgattgat agacaaacac cccaaaccaa
     1921 taaacagata gcaaagatat caagtcccag tacaagtttt tcgaaatatg gaagaagaaa
     1981 ctgcatcaac tctgtcgtca tcctacctct cctcttcttt gtttttcttt gtcttctatt
     2041 tcctgattca agaaataaga aatgactgtt cgaatgataa taataagtgc aagcttcaaa
     2101 atatctgtcc aagtcggctt gataactgac tcgatgatat cagcaacgac tagtacttcc
     2161 aagcttaaca aaatataact tgctagcagt tttcttgtat ctgcattacg agtattacgt
     2221 tctgcatagt ttttcttgac atgagaccaa tcaaaaagtt tgaatagaac aagaacgatc
     2281 ccgacaatta agataccgat agaacaaata tttaaaatag caatgatcca ttctagaaat
     2341 tcaattaaat ttgagcgaaa catatacgct acctactttc ttttaatttg ttcctattct
     2401 tttattgtaa aagaaagtta ccttgttcgt ctatttaaaa gttgttctaa tgataacggt
     2461 tcgcttaaat ttttcttttt cactctccca tttttctccc tccatccata aatcgtggaa
     2521 gtatccaaaa caacgtagca agttaaaata aggcttagtc cgtcatcagc ttttcaaaga
     2581 agcactgtct cggtgctttt tttgtaccca aatttctaaa aatagaacca tacacctcct
     2641 caaatctttt cttcttacct accctatttt tatttacagg aaaatcagac acttcttctt
     2701 ttttgtcaaa atttcctttt tcttctcgtc ctatcatttg gcattctaat aaaaacgaac
     2761 tatccttgga tatagaaaga gaaaacaagg aggcgttttt catgagtaaa aaattaatca
     2821 aagtcggcat cggtttaggt ttgcttgccc ttggcgctgc atatctagga aaaaagacag
     2881 gactattcga agatgacagt catttatatg atgaattcga atcaatctag catcctattt
     2941 agttgaatca ctacggacat acttggaaga cattggagga atcacaatga gaaaaagtaa
     3001 agcaataatg attggtgccg gtattgccaa tatggcagcg gctgtttatt tgattcaaga
     3061 aggcaaatgg aaaggggatc agatcacctt ttattcattg gatgatcacg gttcaaatga
     3121 tggtgcaccg accgacactg tagtggatga atattggaat aaaaaccatc cattagaaaa
     3181 ccaaaaaggc tatattgctc gaggcggtcg catgcttaat taccgtacct atgtagattt
     3241 gatggatctg ttaagtcgaa taccttctgt tactgagcca gggatgactg ctgaagaaga
     3301 tacgagagat tttgatgcaa aacaccgaac ctttgacaaa gcacgtttga tggaaggtgg
     3361 caaaggaatc attgatggcg gaaaactggg attcaataat aaagaccgta ttcttctgac
     3421 taaactgatt gctatgcctg attcagaaga agaaaaacta gacaatatca ctatcgaaga
     3481 atatttcaaa gatgatccac acttttttga aacaaacttc tggtttatgt gggaaacaac
     3541 atttgctttc agaacacgta gttctgctca agaattgcgt cgctatatgc atcaaatgat
     3601 ttatgaattc actcaaatcg aacatctagt gggcgtcaat cgaacacgtt acaatcaata
     3661 cgaaagtatt atgcttcctt tgatcgagta cttgaaagaa caaggatgca aaatcatttt
     3721 gaaccgccgt gtcactgact gggaattcaa agaaacaccg atgcaagatg aaatcactgt
     3781 gactggtttg aaaattacca atacactgac tcaagaagaa gaatacgttg tggtcgatga
     3841 agatacagca gttattttca ctaatgggtc cattacagat tcagctactt taggagatta
     3901 cgaaacacca gctgtagaaa atatggatta tggtgcagct tctagtctct ggaaaaaagc
     3961 cagtgaacgt ttctacaact tgggaaatcc tgacaaattc tttgctgatc gagatgcaag
     4021 cgaatgggtc agctttaccc tgacgacgaa aaaccatttg ttgttgaatg aaatcacccg
     4081 tatcacgact caagaaccag gaaatgcctt gaattcattt atttcaacca caccgatcac
     4141 gccgttaggc caaaaagatg tcaatatgtc tattgtcgtg catcatcaac cgcattttac
     4201 gtctcagaaa ccaaatgaaa cagttatttg gggttatttc ctttatccaa gacgacgtgg
     4261 cgaatttgtg gataaacagt atattaaaat gaatggaaaa gaaatgctag aagaactgat
     4321 tggacaactt tcaaaagtag atcctggacc agtgaatatt cgtgaaaaag aaacagaaat
     4381 tttcgacagc gtaataaata atatccctgt ctatatgcct tatgcttccg cgttgttcaa
     4441 taatcgtgcc aaaagcgatc gaccaaaagt cattcctaaa cattcgacca atctggcctt
     4501 tacaggagaa tttgtcgagc agccttatca gatgatcttt actgaacaaa gtgctgtacg
     4561 ttctggagaa atcgctgctt tccattttgc gggtattcct atgtcccgtc ttgtcaaaac
     4621 accgcgttac gacaaagata ttcctactct agctcgtgcc gcaaaaaaaa tgtttgaata
     4681 atcaagaggt tgtgaaaaag ctgccctgca tcaagtaata agaacaacca ggctaaaaca
     4741 gcttcttatg tttttgattt ggttggactt attaccgcag atgcaagctt ttgaacaccg
     4801 tttaccaaga ggttatgaaa aagctgccct gcatcaagta ataagagcag ccaagctaaa
     4861 acagcttctt atgtttttga cttggttaga cttattaccg cagatgcaag cttttgaaca
     4921 ccgtttacca agaggttatg aaaaaggcgt ttagtttcga gtatgaagaa aaattttgtg
     4981 aaaatggttc ctcacgtttt tgcataaatt tgacttaata ccgagaaact gatcccttct
     5041 gtcataacct ctttttattt atgtctgttt ctttttgaaa acaactattt tttcaacgtt
     5101 tactctcttg ccaatccaac atcatctaac ggatacaact atttcaatga ttaccattac
     5161 actaaaaaat cggaaacaca cctctccgaa cacaaataca taagactata gaatgtttca
     5221 tttaagaaaa aaagcgtttc attatcaaaa aggtaagatc tcctttaaaa tgaaattatc
     5281 tgtagtacta ataatgcaga caattttatg aatctgaaag gagcatctcc aatgaaagca
     5341 gtcacttggc aaggaaaaca gaaaatggaa atcagaaacg tagatgatcc aaaaatatta
     5401 gaaccaacag atgcaatcat ccgtatcaca gcaacagcca tttgcggctc cgatctgcat
     5461 ctttaccatc atggtgaagc agttttggaa aaagggtatg ttgtaggaca cgaaccaatg
     5521 ggaatcgttg aagaagtggg atctcaagtt caaacattga aaaaagggca acgtgtggtt
     5581 atccctttta atattagttg tggtcattgt catttctgcc tgaatcacat ggaaagccaa
     5641 tgtgatgaat caaatccaga tcagttatat ggcgggcttt tcggatttgg caagttgaac
     5701 ggtaatcatt ggggtggtca agcggaatat ctaagagttc cttatgcaga ctctacttca
     5761 tttgttgtac cagataatga tcttcctgat gaaaaagttt tatttctatc agatattttg
     5821 cccacagctt attggagcgt tgaaaacgca ggagtaaaaa aaggcgatac agttgtgatt
     5881 ctaggttctg gtccagtcgg tctattcgct caaaaattcg ctgttatggc tggagcagag
     5941 cgcgtaatcg cagtagatcc agtacagcac cgtctagata aagcggctaa ttacaatggg
     6001 gttgaaactt atttcttaga agacacggca caagctggaa cagaattgta tgaactggct
     6061 aaaggtggcg cagatgtcat tatcgattgt gtaggaatgg acggattaga gcctgtcaaa
     6121 gaaaaagcga aaaatttagt aagcttacaa agcggcacaa tctctccatt acagatggca
     6181 tcgcaagctg tcaaaaaatt tggaacagtc caagtaactg gtgtttatat gactccagct
     6241 tcttcttacc cgctgcaaga attttttatg cgggatatcg ctgtcaagca tggacaggca
     6301 ccaattatcc atctaatgcc aaaaatttac gcaatgattg cagaaggact attcgatcct
     6361 acacaaatca ttactcattc gctgccacta tcagaagcag cacaagcata tgagatcttt
     6421 gacaaaaaag aagataaaaa tataaaagtt gttttgaaac cttgaattca gtaaaaaata
     6481 caggtgattt tctttatgcc caaaatcaaa cgaaataccc atgagatcga aataggaaaa
     6541 gaagaagata tcgaaatacg aggacgtcgc ttccgcttct atttccaaaa tcgttacaca
     6601 ttgatctcac tggctgtcga tttattaacc gggattttct atatttttgg aagccttgct
     6661 tccttaacta aaatccccga tcgatatggc acctattttt atttggctgg tgctattttc
     6721 ttgacgattc gtcctattct cagaatcgta aggaacattt ttatttacga cgaaaaaaaa
     6781 gcaatgaaga gaacaagcaa aaaaaaagag gcatgagaat ttttcaaatc tcatgcctct
     6841 tttttcaccc tatcgtatca ctttgataca gacgagcggt cgtcccactt tcttccttga
     6901 ccattgtcaa aaatgtccac tcgtatcgtt cataaaacgt ggtatgatct gtaattaagt
     6961 aaagtttttc gtacccaagt tgatgcattt cttttctgat ttccgctaat atccgcttgg
     7021 ctatcccctg cttacgcttt tccggttcaa caaacagggc acaaagattt ggtgtcagat
     7081 cagggcggtt atggaagtcg ttttcaatga cgcccgctcc tccgacgatg gtttcttttt
     7141 catctaagac cacataccat tgaggaatac tttccttatt agcgattcca gtacgaatac
     7201 tttcctcata cacaacagca ggaactcccc attttgatga aaaccaattc gcgctttgtt
     7261 ttactaattc tgggtgttct cttaatttga tccagttata tgtaaccaat gtttaaacct
     7321 ccgactaaaa taaaacgttt acaaaaatta tacaagtctt tatgaaaatt agcaatgaca
     7381 acaatagtga tgtaaaaaaa aaaacggaaa acaatcactt gttctccgtt ttttgattaa
     7441 tcaagcatct aaagcttctt ttcttctttc ttggatcgta gctaccttga cgaacggtag
     7501 atagatcaaa atagaaagaa ccaaattgac agcggctagt acaccacctg caatactttt
     7561 tgttgccaat acaccgccaa taattggtgg agttacccat ggaggcataa cggtagctgc
     7621 tggcactaaa cctgtagaag tcgcaaagta agcaacacta actaagacca ttggtgtcaa
     7681 aataaatgga atgaacatga tcgggttcaa cacgataggt aacccaaaca tcgttggttc
     7741 attaatattg aagacacctg gcgcaatcga caagtttgtt acaaccatat atggtttgtt
     7801 tttacgtcct actaaataaa tagctaacag taaacctaaa gttgcacctg ttcctcctaa
     7861 gttgacaaag ctgtcaaaga aaggtttgtt cacgataaac ggtgcgctat gtccagctgc
     7921 gattgcatta acgtttgctt caatggctgg cgcattgatt gtttgcatca aaggatcgac
     7981 catattcgct ccgtgtaaac caaagaacca taagaatggt gtgataaatg caagcagtaa
     8041 ggcagatgga taagagtttg caagtcccat aaatggttct tggaccactg tatagaaaga
     8101 acctacgata tcatcaacac cgaatgcagc aaaaattgca gcaactaaag caaacgcaga
     8161 gatagtgatc attgctggta gtaatgaagc aaatgctttt gcaactgccg gtggcacacc
     8221 atcaggcatt ttgatgatca atttttcatt tccgcttaaa cgtacaaaga tttctgttga
     8281 aataatccca acaataatag caataaacaa accgtttgag ctcataccta atgctccgcc
     8341 taaagcgaag aaagagccta aagaaatcac acctgcggaa acaccgtctt ttcggtagcc
     8401 gttagctaaa tgataagcaa gcaagaaagc aatcaagaca gaaagaatgg caaatgtacc
     8461 gttccaagca gcagccccga atcctttcca tgattcacca ccaaaaattg agttcatcaa
     8521 ttcttggaac ccgggaatcg gtaagttatt gatcattact gctaatgctc ctaagatcat
     8581 taacggcata ctcaccataa atgagtcccg aatcgcaact aaatgccgtt gtgcaccaat
     8641 ttttgatgct acaggtataa aatgtttctc cataaatgca acaaatttat ccataaatct
     8701 tccctctttt ctttactgta gattacgcgt tcaatcgacg atacagatta atgatctctt
     8761 ttgctaaatc tgtaaaagct atagatgtca tcagatgatc ttgactgtgg accgtcaaaa
     8821 gcgtgacttc aacgtgattt ccttgtgctt cttgtgtcag catctctgtt tgagagtgat
     8881 gtgcttggac taaagattcc tctgcatctt tgattttttc atctgctaaa gtgaaatcac
     8941 cttttttcgc agcagcaatc gcttccattg catcgctttt tgcatttccg ccaaacatga
     9001 tcagacccat gatggcttct agattttttt gatcttccat ttattgtatc ctccgataat
     9061 ttttattatt tgtttattag tttttctgct tggtcgagta ctttttcacc attcatcatg
     9121 ccatagtcag ccatattgat tacatctaaa ggaatgcctt taggtgctag cttttcagca
     9181 aattgatttt gcataaagcg tacttgcggt cctaaaagta aaacgtctac atttttagat
     9241 tctagttgat tatccgcatc tgaagcagat acagcaaaga tgtctgcttc cattcctttt
     9301 gcttctgctg ccttttgcat ttttgtgact aggagacttg tactcattcc agctgaacat
     9361 acaagcatga ttgttttctt ttccatttta aacacgttcc tttctatact tacacttgta
     9421 tataagcaag tagcgtgcca actttaaaag cgctttaaaa agttgatatc atcacattta
     9481 aggtctatac caagttgcat acacactaaa aaagaaagcg cacactaaac acacaaatta
     9541 atttttatta ccttttttct tttaacgtgc acaaaaaaag ttgtgacaca aaacttgtca
     9601 caactttttt caaataaacg gtattcaaaa gcttgcatct gcggtaataa gtccaaccaa
     9661 gttaaaaaca taagatgttg tttttacttt gctattctta ttacttgatg caggacagct
     9721 ttttcacaac ctctcaataa acggtgttca atcagctgac cctgggtatt agctcaaaac
     9781 gctggattca caacctcttt tatttcaaat aaccagtaaa tacaatgaac atctacaaaa
     9841 aacttctatt tatttataga tttcgtcagg caaatccaat tctctcttat caatcaaagt
     9901 ctgataccac gaagcagatt ttttcaatgt tctgcttcgt tcatcgttca attcgattcg
     9961 aactaatcca tatcgattct taaatgcatt cattggtgac acacagtcag tgaatgccca
    10021 taacatatag ccttcgcaat ttgcgccttc ttctgttgct ttgatcaagt aatgcaaatg
    10081 ctgtccgata aattcgatac ggtaatcatc ttgcacgatt cctttctcat ccatatactg
    10141 ttcttcatat tctcttccca taccgttttc tgttatcagc caagggatat tatgataatt
    10201 ttcttttaga tacatggcta tatcatacat gattttcgga tagatttccc atcccctgga
    10261 attattcatt ttcttcccag gtaaatcaaa gacttcataa tatcgttcag gatggaaagg
    10321 tgtctcttca ttccattgat aacgaggggc gcgaacgcgt ttaggataat attgattgat
    10381 tccaaggaaa tctactgtat gatgctgaat cgtttctaaa tcctctgttg caggatcgaa
    10441 ataaatcgat tgtttttgac acaactccag gaactcttct gtgtagtatc cttttaccat
    10501 cggatcaaaa aatactcgat tataaaacaa atcatacata taagcagctt tttggtcttc
    10561 tggagaattg gatcgagcat agaccatttc aggatttaaa atacagccaa tccttccacc
    10621 ataatttccg ctgcggtaaa tctctactgc tttagccgtt gctaaaactt tatgataatt
    10681 ccacatcatc cacttttttg tgttttgttc atgcggccag cgaacagcat ccaaatagca
    10741 acgcgtttgg ggaacgattg gttcgttgaa tgtaaaccag ttttttaccc tatcaccgta
    10801 acgatcaaag gcaatttgag catacttagc ataaaaatca actacttttc ttgaagacca
    10861 gccatcatat ttttccaaaa gaaaagctgg gacttcataa tgttccaaac aaatcatcgg
    10921 ttctacgtct gcttcgatca aagagtcgat catttcactt atatgctgag catattcttc
    10981 atctactata cagttctcat aatcagtaaa aaaacgagcc caattgatag aagtccgata
    11041 atgtgtcaga tggatggatt tcatcagatc cacatcctgc tggtattgtt ccataaaatt
    11101 agttgctacg gctgggccat acccattatg ccaaacaaaa tgttcatttt tataccagct
    11161 atctataaac gaatcctgtc cttctttttt tcctttccaa ccttccgttt gccaagcaga
    11221 agatgcagca ccaagaataa aattttttgg aatagtaatt ttcatgattt cctcctgttt
    11281 tttagatccc atcattttcc acacgatttg ctacttttac aaatggcaga tagatcaaaa
    11341 tagaaacaat aatacaaaca agttgcgtaa taactgcacc gatatttcct gctgttgcca
    11401 gataagcatt gacgatcggc ggcattggcc aagggaccat tacaactgct ttcgccgcaa
    11461 atcctaaagc ggttgcaaaa taaccaatcg ttccagtgat aagaggcgtt aagataaacg
    11521 gaatagctaa aattggattc agcatgatcg gcataccaaa aataactggt tcattgatgt
    11581 tgaagatccc aggagcaagg gataacttgg tgatttgttt catatcttcc cgttttccaa
    11641 ctaacaatac tgcaatcagt aaaccgatcg tgactccaga cccgcccata ctcatataca
    11701 tatcccaaaa tggcatagta ataatgttcg gtacttcttt tccagcttca aaagcttccg
    11761 tatttacagc aattgccgct aaaagcaacg gttcccgtat cggcttcacc atttgatttc
    11821 catggatacc aatcacccaa aagacttgcg caataaacat caatagcaaa atacccggta
    11881 acccttgtac gacaccttct aatgggcgtt gtacgacatt gtaaatgata tcataaaggt
    11941 acattcctgt aagttctttg atgataaatc ccgcagccgc gatacccgat acagtcagaa
    12001 tcgtcgggaa taaagcagaa aaactagcag aaacattcgt tggcacggta tcaggcattc
    12061 tcagctgtag tttcttctgt ttcctcaacc agcaataaag ttcaatagaa aggatggaga
    12121 ccaacattcc caaaaataac cctttcgtat ctgtatattg tttagccaat acgttttcta
    12181 ctataacagt ctgattttca ttcactaatt cataaacagt tggattaaca gataaataag
    12241 acatcactgc gatgatacca gggaatacac catccacttt attcagcttg gctaattcga
    12301 tccctatcag aaaaacagca tagatggtca agaaacttag tgtgacatag ctgatggcag
    12361 atgcgatagg ctttaattct tctaaaaaac gcaagaaact tatctgtgct aagccatttt
    12421 ccgagtcaaa gaccattgct gaaaacaatg tgccaaaagc acccgtaata attaccggaa
    12481 tcaatgcagc aaatgcgttt tttatcacca tgatatacct taaattattg attctcgttg
    12541 ccacgttccc taaaacattg gctaatttat ccataaaact cattccagat cccctcattc
    12601 ctgcttcttc tccttgacgt catctctttt tgtctattag tcagcgctta catttcgatg
    12661 aacagaaact atttactcct cttttttacc aaatataaaa caaaaattca tctgatgtat
    12721 ttatcataaa atacatcaga tgaactgtca attagtttct ttttctccta ttattttctg
    12781 taattctctc cgatttattg ccatatggac agtcattgca tcaaacgcag caagtgcatt
    12841 ccgcctttcg atggcctgaa cgatctctgt atgaatatcg atcgtctcga ttttcagatg
    12901 ctggatatca taatttttat taaacaaagc aattgattca ttaatgattg ggataatatg
    12961 cagcaaggct acgtttccgc tagcttctgc aatcatcgta tgaaaatcga tgtctaattg
    13021 aacatgttct tgtgtgcctt ctcgaaaaga agtttcaatc ttttgcgtta attttttcat
    13081 ttggacgatt tgattcttcg ttgcatattc cgctgccaaa cctgccatct taggctccaa
    13141 taaatatctc agttcaaata agtctcgtat catccgatcc tgatcttcaa tcaaagaaaa
    13201 accgagtggg tcattggcaa tccctcgctt atcactgaca aatgtccccg ctccttgccg
    13261 aacttcaagg atattcctgg aagctagagc ccgcacagct tctcgcaccg tgcttcgccc
    13321 cacctctaaa taatcggaca attcatattc attcggcagt ttatctccta cttgcaactg
    13381 catttccttt aaatatctaa ggatcttgtc agtagttact tctactaggg ttttattttc
    13441 ttccaaacca catacctcct tttcatctga tgtatttttt cgtaattatt ataacacgtt
    13501 tgaatcatct gatgattttt tgtatacaaa attgattaat ccgttcatag acatacaaac
    13561 gaagaagata tttaccataa aaaagactag gataaaattc actcctagcc ttcgatcatt
    13621 tttattttga acgacaaaaa atatgatgtt caataacaga cgatcttcat tcgattcttg
    13681 atgtcatctt ttgttttttt taatatatgg ataaaaagtt tgattacatc gtttctattc
    13741 tagaatttgg tcgattctca attcttcttc tggtgagaaa tctaaattat ccatcattcg
    13801 aacactttct tgtaactgac ttaaccgact tgctccgacc agtacacttg taacagattt
    13861 ttgtcgcaaa ttccatgcaa gggccatttg cgctaatgat tgtcctcgtg atacggcgat
    13921 tgtttgcagt gctttgattt tttccagagt tgatccaact tgttcttctg aaaggaatgg
    13981 tatttcctta cgatgtgctc tagaatcttc aggaatacca tgtagataac gatttgtcag
    14041 caacccttga gcaagcggac tgaacgtgat tgcccctagc ccttcttctt ctaatactgg
    14101 agatagatcg tcttctagta gtgcacgaga aaacatattg tagcgcatct gatgaataat
    14161 gaacggtgct tcctttcttt ttaaaatttc agtcattttt tttgtatctt ctccattata
    14221 gttggatata ccaatatata atgcttttcc ttgtcttaca agttgcatca aagcttctgc
    14281 tgtttcttcc aaaggtgtat cagggtctgg gcgatgatga tagaagatgt caacataatc
    14341 tagccccatt ctttggagac tttggtcaca acttgaaatg atggatttac gagagcccca
    14401 ttccccatat ggcccttccc acatatgata acctgctttg ctagaaataa tcagttcatc
    14461 acggtaggct tgaaaatctt ctctaaaaat acgcccaaag tttttctctg cagctccagc
    14521 agggcctcca taattattcg caaggtcgaa atgggtaatt cccatgtcga atgcgcctct
    14581 taaaatctct ctttgattat ggataggatc ataatcacca aaattttgcc ataatccgag
    14641 agataaaatc ggtaatttca aacctgaatg accggattta cgatattcca ttttttcata
    14701 acgtgcacta tctgcaatat acaatttttc tcctcctcat caaatctttt taaaacgctt
    14761 tcttttttct atttttagtt tactacattt taaagaaaag gagttaatca aaatgatttt
    14821 ttcgtatatt tttttgccat ttgtttttta ctcgttgata gatcagaaat tgattacgtg
    14881 cttgctggat ttttttataa cgtttcggtt gttttttgta aaattgctga tgatagtttt
    14941 ctgctggcca aaacgtaccg gctggttcga tttttgtgac gattggttgg tggaaagttc
    15001 ctgaactgtc tagtttctgt tttgattgtt ccgctaattc tttttgtcgt tcgacttgat
    15061 aaaaaatcac tggacgatat tgcttccctc tatcttgaaa ttgtccaaat gtatctgtgg
    15121 gatcagtaat ttgccaatag attgtcagca actcgctata acttatttct cttgtgtcat
    15181 aaatgatctc cacagcttcc acatgacctg tgtaaccacc acttacttga tcataatttg
    15241 gtttttcaac atgtccgccc gtgtatcctg acaaaacaga taggatccct tttttcgatt
    15301 caaacggctc gaccatacac cagaaacatc cccctgcaaa tactgcacgt tcttgatagt
    15361 cagcatcttg catggcatgc ttggaaaagt catatttttg ctccttatca aatccttccg
    15421 taatcaataa ataaaagtcc attacatcag gcgtcagatt cattcggata gctaatggac
    15481 gtagtgttgc ttctagtttt gataactgtt ctttaacgga tatattttct cttatgtcat
    15541 tctttgtctg aatcaatact tttcgttccc aatctcgtgt attcggattc aaaatcagat
    15601 tataaagtgt ttgaatgaat atttgttcct catccataga ttctcctcct ttccgaagaa
    15661 gacaagctag tgactttagc tgtaatttat cataaagtga ggaaataagc gattacttca
    15721 tccccctaca gatttttcgt ataaaaaaga gagaatcctt atcgacagat tctccttttt
    15781 aatcaattta ttttataaat gaaaagttaa tttacctttt atggaaaact attttaaaaa
    15841 ataaacataa atatgcagtt aatgtaacaa gataagaaat aatagtcgtt actgtataac
    15901 ttgtgcttac tttaatatca gcaactcctt tgactccgct tatttgtacc aatccattag
    15961 cattcacctg ctcctttaca ggttgatgat ttatttctac attcgttcct aaataagcat
    16021 atacaggaag tgtgattact tggctcttct cattagttac tctatatatg atagaactac
    16081 cagttgtttt ataaggaagg tttacccatt gactgccaat ctttgcttcg tgttttatta
    16141 tatgctcctt attctccaat gaagcgatag gagaataatc atataaaaaa taatccattc
    16201 ctttagtggt atacgcctct actgtttctt tgtttacaac aatatgtccc ggctgagaaa
    16261 ttattttttg attgaaattt tttttactag aaacagttaa agtgattaat aacaaaacag
    16321 tcataaataa ataaaatagt tctcttgtct tattgtatct tctatttttg aaaaatatca
    16381 tcgttaatat cagagaagag aatattattt gcaagcttaa gatccgccaa ggaaattgta
    16441 acaatttggc tggcgtattt tgcagaagat accatggcac caaactagtt gtagctagcc
    16501 aagtcaaaca cgtaccataa taaatctttt tttctatgtt tgttaatttt ttaaataaaa
    16561 tcaggggaat acataaagat aacagaccaa ctagtcccat tgagtaagta gttaagtcgt
    16621 tgttcatagc ttcagtcaga ctatcgaaca cattagatgc atgagcatat aaatttgtca
    16681 cgctaggttt gttgattttt tggtagagca tttgttcaaa catcggatac caaaaagcac
    16741 tcgtcagaac caatgtcatt cctgccgcac taaataattt tttaaatata gcaataaaat
    16801 ctgcattctt gaaaccttta gaaaccaaac gaataaaaat aaaaatgata attaatgctg
    16861 tactcattaa tgttgatagt acatggctgt acactagcaa actcatactc aatgctagct
    16921 ttacagacgg atacttttct tcttttccag agatgatttg ataagcataa aataatacag
    16981 ggataacaag tgttattgcg atagcttcgg caattgcgga acgatagtag atgtctactg
    17041 tccgataagc agagaaaata tagagacaac taaataaaaa tgccgctttt ttttgtttta
    17101 aaaaccgctc gccataatga taagctacga gtgttgtaca caagttcaat aaccaaacgt
    17161 aaataatata gcttacaatc aaattttttg tcatccaata taacattaca gctggaaaaa
    17221 aagtcagaaa gggataaaaa taatttacac catatccagt ataattgaat gtctcataat
    17281 taatcggact ggtaaaaata gtcgataacc ccttgattcg agataagtga aaagaaagat
    17341 ctccagtggt ataaatactc tgtgtatttt tgaaaaagat gaaagaatat atactactaa
    17401 ctataattaa tccactcaat attactactt ttttatttat tttcattctt cttgtttatc
    17461 tcctttttag tcacatatcg agaatctttc aacttattag gtgcaaatcg tttaaaactt
    17521 gaatacacca ctaaacaata agtcagaata ctgatccata aagccatgag agcagttgga
    17581 gtatattttg ttgtaatttc tatcttattt tctccagcat ttacatccac attcactaag
    17641 ccatgttttt tataagagtc atttacgact ttaccgttta cttttacctg tgtacctaaa
    17701 taagcatata ctggtactgt cactttttgc tttttgttgc ttgccactgt gtaagtaatg
    17761 ttattatctg cagctttata cttccctttt tcccacgatt ctcctacttt tacttcatgt
    17821 ttttctaagt gaggcctttc ttttatcgct tctgttgggg cgtaatcgta taatcctccc
    17881 atatctgatg ttgtataacg tgggagtgtt tcttcattga taacaatatg accgtgtttt
    17941 gattggataa cttttgcgta attttctttt gctgctatag acgtgacgag tagaaacacc
    18001 acagtagcgc caatgattaa ccatgaatat tttttattca tagtcttttt cataaaaaca
    18061 agcgttatga tcattgaaga aaaaaagatt tggataccta agatacgcca aggaaactgg
    18121 agtaagctta tcggtgtttt ttgaaacaaa aaccatggaa ataaattagt tacgcatacc
    18181 catgtagaaa ctacgccaac atagatgatt ttttcttttt tctccatttc tttcagtatg
    18241 aataaaggaa aaatcaacgc aaccaatcct aataacccca tagtgtatgt tgtaatatcg
    18301 ttatttaacg cttgaatgat actatctgaa atagaaagtg cttctagctg caaatctgtt
    18361 ttgaagggac tgtgtatttc ttgataattc atctgctgca acataggata ccaaaaatag
    18421 ctagttaatg cacaagtcaa aaaaacagcc tttactaaat tcacgaccac aaattttata
    18481 tcgtttttta tttgcttgct taaaaacaaa cgaactataa tcatacaagc aatcaatgca
    18541 ctacacaata gtgtagttaa gacatgagta tacacaagta gacttatact gacagataaa
    18601 gcgatgactt ttttttctct attgaatatc aatccataag cataatataa aacagctggg
    18661 ataatcgtaa gagcgattgc ttctgcaatt gcggatcgat aatagatatc aactgttcga
    18721 taagttgcaa aagtatataa acaactaaat aaaaaagcag catctatctt tttaaaaaac
    18781 ttttctccat aataaaaact aatcagaatc gtacaaatat ttaataacca aatatataga
    18841 acataactta atattaagtt atttgaaatt ccataaaaaa cgactgcagg aattattgta
    18901 agaaaaggat aaaaatagtt cagtccatct ccataattgt taaatgttgt atagttgatt
    18961 ggcccttcaa aaatagaaga taaccctttg attcgtgaaa gatgaaaaga taaatcacct
    19021 gttgtataaa tttcttgagc atttctcata aaatttactg aatataaagt actaagtaag
    19081 ataagaacta ctaaaagaaa tgctttttta cttattttca tagatttgtt gttctccttt
    19141 tttaattcta ttttcagtat actagaattg tcttttttta ataagttttg cgttaaa
//
LOCUS       DARBDV010000059        19126 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000059 DARBDV010000000
VERSION     DARBDV010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.059"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..703)
                     /locus_tag="SCM56_001879"
     CDS             complement(<1..703)
                     /locus_tag="SCM56_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1110643.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFT"
     gene            complement(717..1641)
                     /locus_tag="SCM56_001880"
                     /pseudo
     CDS             complement(717..1641)
                     /locus_tag="SCM56_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            1758..2837
                     /locus_tag="SCM56_001881"
     CDS             1758..2837
                     /locus_tag="SCM56_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEG1110644.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            2869..3462
                     /locus_tag="SCM56_001882"
     CDS             2869..3462
                     /locus_tag="SCM56_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1110645.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(3572..3799)
                     /locus_tag="SCM56_001883"
     CDS             complement(3572..3799)
                     /locus_tag="SCM56_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG1110646.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(3902..6490)
                     /locus_tag="SCM56_001884"
     CDS             complement(3902..6490)
                     /locus_tag="SCM56_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEG1110647.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(6510..7169)
                     /locus_tag="SCM56_001885"
     CDS             complement(6510..7169)
                     /locus_tag="SCM56_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEG1110648.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="SCM56_001886"
     CDS             complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="SCM56_001886"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEG1110649.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(7942..8808)
                     /locus_tag="SCM56_001887"
     CDS             complement(7942..8808)
                     /locus_tag="SCM56_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEG1110650.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            8917..9558
                     /locus_tag="SCM56_001888"
     CDS             8917..9558
                     /locus_tag="SCM56_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEG1110651.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="SCM56_001889"
     CDS             complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="SCM56_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEG1110652.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(10999..11898)
                     /locus_tag="SCM56_001890"
     CDS             complement(10999..11898)
                     /locus_tag="SCM56_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEG1110653.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(11900..12697)
                     /locus_tag="SCM56_001891"
     CDS             complement(11900..12697)
                     /locus_tag="SCM56_001891"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEG1110654.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(12675..13379)
                     /locus_tag="SCM56_001892"
     CDS             complement(12675..13379)
                     /locus_tag="SCM56_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEG1110655.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            13510..14085
                     /locus_tag="SCM56_001893"
     CDS             13510..14085
                     /locus_tag="SCM56_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEG1110656.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            14203..14880
                     /locus_tag="SCM56_001894"
     CDS             14203..14880
                     /locus_tag="SCM56_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEG1110657.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="SCM56_001895"
     CDS             complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="SCM56_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEG1110658.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(16943..17941)
                     /locus_tag="SCM56_001896"
     CDS             complement(16943..17941)
                     /locus_tag="SCM56_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEG1110659.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(18167..18820)
                     /locus_tag="SCM56_001897"
     CDS             complement(18167..18820)
                     /locus_tag="SCM56_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEG1110660.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         5525 a         4136 c         3324 g         6141 t
ORIGIN      
        1 acgtaaagct tgctgccaaa ttagcgattg aaactaacga ttgctttcta ggaatcaagg
       61 acaagcgaat accatttgac gattgatttg cttgctgatc ctgtacattg cggacccccc
      121 acatcgtccc gtagagaatc gccattccaa tcagagtgaa gaaataaaaa cttttgaaac
      181 tcatattacg acctgcatga acttcttgaa aagatacttc ttgttcaaaa acagtcatga
      241 tgggcgcgag atcaacgttc tccacttctg ccactttact cgaaattata ccaatacgct
      301 gtaaaaattg atccatgacg tttttcatca acgtctgact cattcctgct tgacccacat
      361 ggagcgaaac agatgaacca tcaatgagat aaaaaccagc caaatcatct gcatttagct
      421 tttcattcgc ttctacttcc gatagttcct gtacttcaaa taatggttta tcattgactt
      481 cgacttgttc taagactgtg gtaaaatttt ccgaaagcgc tgtttgatcc gctttgatta
      541 caattccagt attaatcgtt tcgagattgc caccttgatc aatctcgcca aaggccgcca
      601 taaacatcaa tcctaaaaga attggaaagg ccaatgtcca aaacaacatg gatttatttc
      661 gcagcagtat ttttgtgcga tatttataaa aatgccaaaa cattatttcc cctcctctag
      721 tctctcaaat ccttgccagt aatctctaaa aagacatcat tcaaggacgc ttctttggtt
      781 gctatttttc cgtaactgat cttttcttct tgtaagattg taagaatcgg caacaatact
      841 gtatttagtt cagtagttgt tacttggagc tgaccttttt tataagcgat tccatcagaa
      901 gctattgttc gctgacaaca cgttttctgg gagtaatggg atgtcgatcg tcaaacgatc
      961 cgccgtccgt accatatctt ttagagattc tttggttcct ttagcaatca cctgtccttg
     1021 atccatgatc acgatctggt cgcaaagaat ttctacttct tccatgtaat gtgttgtata
     1081 gacaatggtt gctccttgtc gattcagctc cttgatactt tctaaaattt tattccgact
     1141 ttgggggtct acagctactg tcggctcatc taaaaaaatt aaccgcggct tgtgggcaat
     1201 cccacatgcg atgttcaacc gacgtttcaa gccaccacta agttctcctg gacgaaacga
     1261 aacatatttt tctaatccca ctaattgaat cacctcttca atcaaggctt tccttttctc
     1321 tttttcggaa acatataaac cgcaaaaata atcgatattt tcttgcacac ttagttcgtc
     1381 aaagaccgca acgtcttgag gaacgacccc aatcattgcc ttgacagcat aattttcagg
     1441 agtcattgtt gtcccgaaca atttaatggt tcctttgtca taatttaaca aagaaagcat
     1501 acagttgatg gcagtcgatt tcccactgcc gtttggtcct aacagaccta agacttctcc
     1561 ttcttgtact tgcagatcaa aatgattcaa tgctacaaaa tctccatagc gtttgactaa
     1621 tttatcaatt tcgataatca tcatgaaaaa ctccttccaa atttcttgtt gatctgacta
     1681 taataaagtg caagaaaaga aactagtgac gattggctaa accacacatg acatttgtca
     1741 caatgaagga gtctgctatg cataaatctc ggctactcaa cgaaactagc tttctactgg
     1801 ttatctcatt gcttttgctg gtaagtcaag gattcgttgc tatgcaagtt atctatttat
     1861 tgatcgccgt tttattcact ggtgtcagtt atttactgcc tacaaagcca aattggattt
     1921 tccaagttat tctatttctt ttatgtgcct ttttacctga tcttgtcctg tttctgccag
     1981 cgacgatgcg agtgatcgcc cctgaagaaa gccaccttga ttacttgttt ttgttaggtt
     2041 ttttgttgat ttcgacggtt ggtaatcatc cccccatcaa ggtcgccttt gtcgtaattt
     2101 tgctgttttt ttccttttac ttacgaaaaa aagatcaacg aacgaccgat cttgagaaaa
     2161 agtacattgt ctccaaagat actcattggg aaaaacaaca agagttatcg gaacaaaata
     2221 aagcattggc cctgtcacaa gaaacacaat tagccttgca aattgtggaa gaacggaacc
     2281 gaattgcccg agacatccat gacaatgtcg gtcatctgct gtcaagtgcg attttacaaa
     2341 ttggtgcgct tgaaatgatc aatcaagaac caaaactaaa agaaccatta gaaaatttaa
     2401 gtgccaccgt tcacactggg atggatcgga ttcgtcaaag tgtccatgac ctccatgaga
     2461 catcgatctc ttttcaacac tccttagagt ttctgatcgc tgattttcct tcttctgtta
     2521 cgattgaagg aaacttgttt gaaggattga atgaaacgca gcaaaactgc ttcttaatgg
     2581 tcatcaaaga agccttaacc aacagtatca aacatagtca tgccaaaacc gttgttttac
     2641 attttcgaac gttgccggcc ttttatcgct tgcaaatcac gaatgatggc accagcccac
     2701 aaaaaaattc tgctcccggt attggtttga aaacgatgcg tcaacgtgtt caagaacaaa
     2761 agggacaagt ccatattttc caagaaaacc agcaatttca gttaacgatc attttaccaa
     2821 gggagggaca ccaatgataa cagtagtcat tattgagatg atccatttgt gacgaaatcg
     2881 ttacaaacca ttttagaaag cacgaaagag atccgtgtgt taggcattgg tcattgtgcc
     2941 aaggatgcgc tagtcttata cgaaacccat cgtcccgatg ttttgctgac ggacatccgc
     3001 atgcctaaac aaacgggcat cgatgctgcc aaagaaattt tggctaaatt tcccaaagcc
     3061 atccttttgc ttttgacgac attcaaagat gaagaataca tccgcgaagc attttccatc
     3121 ggtgtcaaag ggtatttaat caaacagaac ttgcaagcga ttattccttc tgtcaaatcc
     3181 gcttataatg gacaggccgt atttggtaat gaaatcatcg agactttcac tcaaatgatg
     3241 aagaataaac cgacgattga tagtcatgct tatgcttctt ttagtgaacg tgagcttgca
     3301 atcatcaaag aagtagctgc cgggaaaaac aataaagaaa ttgcagatgc cttgtacttg
     3361 agtgacggca ctgttcgcaa ttacatcagc caattactag aaaagctaga tttacgggat
     3421 cgcacgcaat tggctatcta tttttatcag catttgcaat aagtcttatc taacacaaaa
     3481 aaacctgaat gatgctcctt cgcatcattc aggttttttt cacatcttct attcaattgt
     3541 cccgcaaacc atttcctcac gattgaaaga atcaaggatt cgcagggctg ccaggattcg
     3601 ttggattgtt cggcgtgtta ggcggaaaaa taggcgcctt gtctaacgtt ttttctagtt
     3661 ttgattcttc acgatcgtct cgttctacat caggactttc attcaatttg aattcttttt
     3721 ccatctcatc taacttttta aagtcattgc cagattcttt tgctaatttc gctaatttat
     3781 cttcggtttc tttagccata aagtaacgcc ccccttttga tttcagctta gcatatcaca
     3841 ctgaaaaaac aaaagaaaac gctaaccaac gatcattttt agctatttct catctttaaa
     3901 atcaagcatt ttttgccggt ataaaatcgt agggagaact gttttccata ccaatcatcg
     3961 gatctactaa ctggttatcg atcacttctg ctgtaagaac gaaatcttta gaaacagcag
     4021 gttctacctc ttctttcttt tcttctccaa caaatgaaga aaacagatct ggttccactg
     4081 tttccattac ttggtttttc gtttcattcg tcccactgtc atcttctaca ggtagctttt
     4141 cttttacaac ccgtggctct tttgtatagt tcccatcttc aaatggatca tttccgacca
     4201 aagaatcttc ataagctgct agtttggtcc ggagagtgat attcatttta tcattatcca
     4261 tgatccttga tttcaacgta gtcatccggc ttgctgattc attcgctaca caagtgacat
     4321 aggcttgctc ttcccctaac aaaatcaaca gttccttact gcgagtcact gccgtgtaga
     4381 gcaaattacg ctgaagcatc cgctgatatt gatggaccat gggcaaaata accatcttga
     4441 attcactgcc ttgtgattta tggattgagc aacaataaga aagagtgatt ttattccatt
     4501 cgtttctttt ataagtgacc tcatttgcat caaactggat aactaattca tccactttat
     4561 cttcagaatc ttttgccaag atgatacccg tgatttctcc catatctcca ttgaatacat
     4621 tcaattcggg agtattgact aattggagga ctttgtctcc tatccgataa acggtgtcgt
     4681 tccaatgtac ttcctttttt ccaggtttcc ctggattgaa tatctcctgc atcattttat
     4741 ttaacgcatc gatccctgca ggtccccgat acattggtgc taatacttgg atatcttgtg
     4801 cacggaatcc cttatttttc gctttttcca caattttaga gatcaagggt tcaatttgat
     4861 aagcatcact tcggaaatag gaacgatctt tttgattctt tgtgaaatct tttggcagac
     4921 gtccttcttt gatctcatgg gcaagaggaa taatactaga accatcacct tgccgataaa
     4981 tctgattgag ttccattttg ggaatttgtt cgatttccaa aagatcatgg agtacttgac
     5041 caggaccgac agagggaagc tgatctttgt ctcctactaa gataacttgc atattcgtcg
     5101 gaatcgcttt gaataatgtg ttagccagcc atgtatcgac catagacatc tcatcaacaa
     5161 tcaaaagtcc gccttctaat tctttcgtac tgactgaagg tgttttttcc cgtccattca
     5221 gccccagaag acgatggatc gtactggcag gcagacctgt cgtttcgttc attcgtttag
     5281 ctgctcggcc agttggtgct gcaagtagaa taggaaaaat ttcttgggta tattttgaag
     5341 gatccaattc cagtccgttc aactctgcaa aaagattgac gattccgttg atcacagtag
     5401 ttttccccgt acctggtcct cctgttaaaa taaatagagg ggaacggatt gcttcagcga
     5461 tagcttgttc ttgcgaaggt ccatactgta tgccgaatat tttttcgata cgccgaatat
     5521 tcttctgcac ttctttttca ggataatgga tttctttttt acgactgaga aggcgctgga
     5581 tcgaattacc gatcccccat tcagaaaagt acaaactgtt ttcgtaaaga ttagtctctt
     5641 cttgttgtat ctttccttct tcaacaagtt ggataattac agtagcaacc tgatcaggct
     5701 ggatttcaat tgggcgactt gtttctaaaa tacggatcgt ttgctgaagc aatgtctccg
     5761 ccggtacgta tgtatctcct gtttgaactg catgctgaaa aatctgatgc aagatagctg
     5821 ctcgaattct tctcggtgaa tctgctgcaa taccgatttg ttcggctaga ttatctgcac
     5881 gtttgaaccc gattccttct atgtcttcga ctagctggta cggattttct tcgatgatat
     5941 ccaaagactc gttcttatag gcctggtaga tactgaaagc taactgacta ccgaacccgt
     6001 aacgattcag tcccatgatc acttgatcca tcccgtggtt tagccgaatc gtctcagcaa
     6061 gcatttctct ttttggcttc gtcaaaccgg agatttgttt taataccgaa ggatcagcaa
     6121 tgattttttc gatagcatct tctcccagca attctacaat tttttctgct gttttcttcc
     6181 cgattcctgg gaatttttcg ctggaaagat acgctacgat cccatttgca gttgttggct
     6241 tttcttgctc atatctttcg agtttaagct gccttccgta tttaggatga tccactaatt
     6301 ctccataaaa acgataagtc tcattttctt gaatatcgcc gaaacttccg gtgactacaa
     6361 tctctttttc acggtaatca gctgtggttt cactgatgtg taccaaaagc actttgtaaa
     6421 aattactagg attttgaaaa aaaatcgcgg caactgtacc aacgaaataa ggcatttctt
     6481 ctggctgcat cggaagccgc ctccttcttc tatagtaaag gttctggtgc tgtttcagtt
     6541 cctaaaaaat ctgtttggtt ccatactttc agttcatatg gcaataatct gcaaggctct
     6601 tcagtctcta aaatcgtcaa actactattg aacaatccac ccatttcacg caactcactt
     6661 agctcttttc ctgtcatcca ttgaatggct gcagttaaag aagctccatg tcctacgaat
     6721 aaaagtgggc catctttatg ttgggcaacc gcatctgcta caacagtttc gatacgtgtg
     6781 attgccgatt caatcgtttc tccgtgaaaa ggagaaggat catacagatc taaatgatta
     6841 cgcaaattcg gcagattatc cggatatttc tggtacattt catcgatcga ctgaccttct
     6901 aaagtaccca gccctagttc tcttagctta tctgtataaa aaatctctac cggacgagca
     6961 agctctttgt tgataccttc tgctgtatca cgtgctcgct ttgctgtact ggaataaatt
     7021 gctttaaaag gaacatattt tacctttttc ccaaatgctt tgatttcttc gtaactttgt
     7081 ggcaaaagcg gagaatcgcc attgctccct tgaaaacgtc tttcctgatt ccattgtgtc
     7141 ttcccatgtc gggtaaaata tagttccata cgcagcaaac tcctgtcttt cttttatttt
     7201 actctataat aaactgtcta atccagcttg tcattttcat agtgatgcgt caattctaaa
     7261 ttagggaatc cttgctggcg aagtgcttca tacactatca ttgcagctgt attcgataaa
     7321 ttcaatgagc ggacatgctc atcattcata gggatgcgta aacacttttc ttcgttttca
     7381 cgcataaatg tttctggcaa accggttgtt tcttttccaa ataaaaagaa gtgctcttct
     7441 ccatcagcaa aattttcgtc actatatact ttatgggcaa attttgagat caaatgtagt
     7501 ttacgattac ctaaaaatgt taaaaaagct tctaaattac tgtggtaagt aatatttaca
     7561 tcattccaat aatccaaccc tgcccgtttt agatgtttgt catcagttga aaaacctagt
     7621 ggctcgatta aatgaagcgg agtattcgtg gctgcacacg tacgggcaat atttccagtg
     7681 tttgcaggga ttaatggttc aaataaaacg atatgattca tcaggatcat tcctctcata
     7741 tttatgttcg tttctatttt ctgacaattt gatttttgca taaaaaaaaa cgtatcaact
     7801 cggtgtcagt cactgcgagt cgttacgtta gtgaagctct tctcactaac ttttatcaga
     7861 acaggaacct gataaaaacc aatgaaaaaa caacttgttg ctaatataac accctcaact
     7921 tttttttgca acctctactc attaaaaact gctagaaaaa cgttttctct tagttccttt
     7981 taataattct atgtcaattg taatcttctt caaaggattc ttatataagt ctgctttgat
     8041 tcgctcttct acctgccatt ctaaaggtga catggccaaa agatcttttt gattttcctc
     8101 tggtatctca aattcatagg ttacacgctg acgttcagtt aatttcattg cttcagcaaa
     8161 cttggctact acacgttcat tggaataact ctgtttttca ggttgattcg gataaaaagc
     8221 ctgacgtagt tcctttaaat aattttcttg tggcaccacc ttcaacactt ctccatcttc
     8281 tgagagtaca cgttggaatt cttggtaatg agaaggcgaa aagatattta aaaccgcgtt
     8341 gaatgtttga tcagcaaacg gcaaattggt caaatcagct acacaccaaa atgcttcggc
     8401 agattgttct gtagctgcgt agactccttc ttttgaaata tcaaatccaa cagcattttg
     8461 aatctttttc aattttgaaa gttcattcaa gaagctacct tctccgcagc caacatctaa
     8521 tagcgagact tcttcaggta aatgctgcgc aatagtctca ataacgggct gatacatgcc
     8581 agattggatc attctacgcc ttggaacgaa cattccttga tcgtattctg ttttaaccgc
     8641 atgggataaa aaataaagtg ttcctttttt ggaaaggtca aaacggtgat tatttttgca
     8701 tatcaagcca ttatcagact tttttacttc ttcttggcaa accggacagc ggaacagctg
     8761 atgattttct gccaaaaact ggcgtcctcg gtcaattttt tttaacatag ttccccctca
     8821 tttctgtttt atcttaccgt tagatgctca ctttggcaac tgctaaaaat atgatacaat
     8881 gcctagaaga aagtgaaaga taaaggagaa gattagatga tcaaagaatt ttgcgctgaa
     8941 aactacacat ctattcctct agcaattgct agaggagccg atcggatcga attgtgtgac
     9001 aacttagcgg tcggtggcac aaccccgagt actggtgtga tcgaagaaac gttaagttat
     9061 gctggggaaa aagatatccc cgtaatgacg attattcgtc ctagaggggg cgacttcgtc
     9121 tataacgata tcgaattgaa aatcatgcat actgatttga ttgaagcaaa gaaattaggg
     9181 acggatggcg tagtatttgg ttgtcttact ccttcaggct ggctagacga agaggcctta
     9241 gaactattga tcgaaacagc agaagggttg cagatcacct tccatatggc ttttgacagt
     9301 attcctgaag atcggcaatt cgaagcaatc gattggctgg tagaacatgg tgttgaccgt
     9361 atcttgactc atggcggtcc tgctgacaaa actattgtag aaaatttccc acggcttaaa
     9421 gaattgattg cctatacaga tggccgctta atcattttgc ctggtggtgg tgtcactgca
     9481 gagaatgcag cacatgtagc taaagaattg aacgtaagcg aagtacacgg aaccaaaatc
     9541 gtggatcttc agttataatc ttataaacaa aaaaacattt gcaacaggca taatgcttat
     9601 tgcaaatgtt tttgtttttt tacataaaat agcccataaa taaagaagca atattgaaat
     9661 aaatcagtac actcgtcaaa tcgctcaaag tcgtgataaa gggaccgctt gctactgctg
     9721 gatcaaatcc tagacgatcc attagcatcg ggattaaact acctgcaaga ttcgccactg
     9781 tgattgcaaa tagcatagcc atccctatga caaaccccaa gataaaatta tgtttccaaa
     9841 aacctacgat tagaaaaatc gtgatccccg taacagctcc tgtgacaacg ccggtcaaga
     9901 cttcagccaa aacaagtcga acaaatccat gatctttctc atcagaaatc gcaagtcgac
     9961 gaacagccac agctagcgac tgggttccag cattaccggc agttcctgta atcagcgaaa
    10021 tgaagactgc taaaatactc gcttcactta ccaagtcttc atagtgactg attaaagaag
    10081 cggtcgacat acccaaaaac aacagagtga tcagccaagg caaacgtcga gacgctgcct
    10141 ttactggatt ttcattaatt tcttccacat tgaccccggc aagtcctgaa taatcgctag
    10201 cagcttcatc atcaataacg tcgatgatat catcgactgt gacgattcct aacaaatgat
    10261 catcataatc ggtaacagga agagctaaga agtcataatc tctaaatgtt tgtgccacat
    10321 cttcttggtc gtcgcctaca tgaactgaaa gcactcgttc gctcatcaga tcagaaatca
    10381 ttgcgtcgtc atcattcacg attaggtcgc gcagagaaat aacccctact agctgacctt
    10441 cttgattaac gacatagatg taatagattg tttcagctac atctgcttcg ttcttcagca
    10501 cgtacatcgc agagcgaaca gtttgattgg ctacgatcga aacaaattca gttgtcatga
    10561 tcgcgccagc cgtttcatcc tcgtaatgga gaagttcctt gatttcactg gcatcgtctg
    10621 ccgacatcaa acttaaatat ttagcaattt gctttttatc cagcgtatta agcaagtcaa
    10681 cagcattatc tgtatacatc tcagaaagca tatcagctgc atagctagga cgcatttcat
    10741 ctaggtattc tttcatatgt tcgttgtctt cttcaatcac atcgaacata tccgctaatt
    10801 ctttaggcga cagataggaa tagatcagct gcctgttctc ttctgtaaga gattggtaga
    10861 attgcccctg ctcatagata tgcatctcta aaaaactgtt tctgaatgcc tgaatattat
    10921 tttcccttaa atcttgagtc aactgggcaa atctttcctc taattcttga ttttcatcca
    10981 aaccaatctc acctcgcctt attcttcaaa ccgtctcaca actgcctcca tgtctgcggc
    11041 taaaggctgc ttgaactcca gcaactcttc tgtaaatgga tggtaaaaac gcagatcata
    11101 acaatgaagg gcttgtcttt ttattcccag atccattcgc ccgccataca tatcatcacc
    11161 gagcagggca cagccaattg cggaaaaatg aactcttatt tgatgtgtcc ttcctgtatg
    11221 cagctggata tcaactaacg cgatttctgt tagtcgcttc tctatccagt actcagtctg
    11281 tgccgaacgt ccttcatcag agaccattcg cttcaatagc gaagaataat ctcgggcgat
    11341 cggcaaatcg atatgtccat gtgccgataa atctgaaagc cttccactga ctaaggcctg
    11401 atattttttc acaaacttct tttcacgaag ttgcatatct aattttgcgt gcgcaaatcc
    11461 atgctttgca aaaagcatca aaccagatgt atcccgatct agtcgcgtca ccacatggat
    11521 tacttggttt tcgtaacctt gttttttata ataagccttt acacgattag ccattgtccc
    11581 atttggatgg tattgggcag gaatagacga aacgcctgct ggtttattta ctaccagaac
    11641 gtgttcatct tcatacacga tatcgatcgg tgtttcatcc aaaagtacgg tttcatgttc
    11701 tccttcagca ggaatcacaa tggttacctt atcatcctta gctagagaaa acaagacatt
    11761 ttctacttga tcattaactt tgatttgacc gccttgaaat tttattttcg ctaacagacc
    11821 ctttgagatc cctttttctt ttaaaaaata ttttacttgc tgagaatgat ctttctcata
    11881 atcccatgta aactgcatat caatcctcac taataaaggc atctttcaca cgatgccaaa
    11941 aatgcatatg tcgataagaa gcaaaatgga tccgttcttc tgctattcga taataaattg
    12001 agcggatatt tgcttttgcg acattcaatt gatcaaccgt tacaagataa tcttctgtat
    12061 tttccaattt tatttctagc cattcatcat gggcaatcac gatcggagaa cccaaagtac
    12121 gaaacactcg attattcagt gaagcaatct ctgccaactg aaaggcattg atactaggat
    12181 ggatcacagc accgccgaca cttttattat atgctgtcga accagtaggc gtagaaatcg
    12241 tcaatccatc tcctctaaaa ctttcaaaca gctcgttttt gatgtataca tcggctacca
    12301 tcgtacgatt ggcccgttta atcgttgatt cattcagtgc taaaaaatgc tggtccggtg
    12361 tttcatccaa ataactgatc cgaacatcca gcaagggata actaacactt tgttcccggt
    12421 tcgttcgtaa gctgtctact agttcttcca gctcgtaatc ccgccaatcc gtgtaaaacc
    12481 ctaaatgacc tgtatgcacg cccaagaaac gcacatcatt cagacaatga ctgtagtgat
    12541 ggaacgccga caataatgtg ccgtctccgc cgactgagat gaccaattct ggctcatgat
    12601 catctcgttg aatccctgct tgttctaaaa gtgcgttcaa acgttctgtc acttctatag
    12661 tctttgcttc tttattatga acgatcgcta ctttcatccc ctgcctcctc tctttcgctt
    12721 tctttcttca aaagcgttcg atagtagaca tcatctgatt ttcctcttcc atgggagaat
    12781 aaccgctgtg cttcttggat ttcttcacgg atactggaca tctcttcatc caactgatag
    12841 gcagcttctg ccgcacgctg aagtcgttca ctcatttctt ctggaaaaac gccttgatat
    12901 ttataattca gcgaatgctc aatcgttgcc cagaaattca tcgcaagagt ccgaatctgg
    12961 atttccgcca aaattttctt ctcaccgctg atcagctgta caggatactc gatcaccaga
    13021 tgataagaac ggtagccact tgctttttta tttgtgatgt aatctcgttc ttggagaact
    13081 ttcatgtctt ttcttttacg gatgatctca acaacttgat ggatatcttc tacaaactga
    13141 cacatgatcc ttaacccggc aatatcagac atttcttctt ctaaacgtcc cattggaata
    13201 tgccgtaatt gtgattttgt tacaatacta tcgactggct tgactctccc agtgacaaat
    13261 tcaatcggga catgtcgatt ttgttcacgg aactgtttgc ggatcccccg cagtttcact
    13321 ttcaattctg atacagtttg ttcatatggt gctaaaaatg cttcccaatc tcgttccata
    13381 gctgatacct ctacttcatt aacattcatt gtttattcta ccatatatgc aaagtttttt
    13441 cgtactcttg atttatgcat tgaccaaaaa aagaaaatta ggcaaaatag acaaaaagga
    13501 gtcgatcaaa tgacacaaat gcttgaaatc gaatataaat ctatgctgac aaagcaagag
    13561 tacgagatat tcatcgctca ctatcaatta acagataaag atttccgtgt acagactaat
    13621 atttatttcg ataccgttga tgaacagctg aagaaacaaa attgtggcct acggatccgt
    13681 ttcgttggtc atcaagctga atatacatta aaaacacctg ctgaaaaagg gcgtctcgaa
    13741 acaacggata cctttataac agaagaagca gaagcattta tcttcgaaaa acgtctgcct
    13801 cgttcaggcg cagtttttca aaaactttct gatttgaata tcgatcctgc ttcattaatc
    13861 caaaccggaa agcttacaac aaaacgggct gaattcccta ttgaagaagg acttttagct
    13921 attgatgaaa gctggggaga caatctacac gattttgaac tggaactaga ggtaggggat
    13981 gcatctgtgg gaaaaattgc ttttataaat tttttaaaac gtttttcttt accttatcgt
    14041 cctgctaaga acaagatcca acgtatgctt gaagctaaaa attagaaacc tttcattttc
    14101 acaaggcact tgagttcatt tttttacttt tttctgttaa gttataggat gtgaaagaat
    14161 ctagtcacta gttgtataga atctattttg ggggagattc atatgatcga aatctattta
    14221 tttgttaatc ctttagggga atactgtttc gaaatggaac aacagttatt acaatttatc
    14281 gaagaagagt acgggacacc atcaaaagaa aagatgcaat ttcgctttct tcctttggtc
    14341 aatttgcaga caattgggga tgtcatgcaa caaaaaggaa tttctcagaa caatcttgag
    14401 gaacgtaatc acttattctc aatgacttat tctgcagcat tggactgcaa agcggcgcag
    14461 tttcaaggaa agaaaaaagg ccgccgtttt ttgatcaaat tgcaagaagc agtcggctgt
    14521 aatgacatac catattctca agaacttgct gaatccatat ttgaagaaat cggcggcgat
    14581 ttgaatatgt ttaaagaaga tcgtcagtct gacttcgtta aagaagtatt cttttctgat
    14641 caaaaaattg ctcgggaaat gggcatcaca aaacaccctt ctgcagtcgt ttacaattat
    14701 gcctgcgacc gtgattttgg tgttttagta gaagtaaaag gtacaagtag cctttggaga
    14761 atcacagagc tgtgtaaaac aacagataac tcatatcaag tttttaatgg tgaacattta
    14821 catacaaatg attctcagtt ccattcaaga actagccatt tacacttgct ttctaattaa
    14881 aaaaatacgt cgactccctt acaagagagc cgacgtattt ttttcgtttc tattcgagaa
    14941 aatcaaaaat agaaagcctt ataaacgatc gatcaattct tctaattcat ttagacgttg
    15001 ttcaaacatc gacattgcat cttcaatata tgcagcctgt gtcatatcaa ctcctgcttt
    15061 tttcattact tccacaggat aatcgctgtt acctgctttt aggtaagcca agtagttctc
    15121 tagtgcctct ggttcctggt ttagtatttt cttcgccagt gctgaagcag cagaaaagcc
    15181 agtcgagtat tggaaaacat agtaattata ataaaaatgc ggtatccgtg accattcgaa
    15241 tttaatttct gggtcttctt cgactgctgg accataatat tttgcattca atttaccata
    15301 actatcactt aggtattcac tagtcaatgg cacacctttt tcatcttccg tatgcatgaa
    15361 atgctcgaat tcagcgaatt gtgtctggcg gaaaactgtt cctttaaacc catccaagta
    15421 atggttgagt acataagccc gtacacgagg gtctttttct gtttccaata aatactccgt
    15481 taggatattt tcattcgttg tcgaagcaat ttctgctaaa aagatggagt agtcgccgta
    15541 cacataaggc tggttcgaac gagtgaaata actatgaaca ctatgtccca tttcgtggac
    15601 aagcgtaaat agctgatcca gcgtatcatg ccaattcaat aaaatatatg gatttgtgtc
    15661 atagcttccc gaagaataag caccgcttcg tttcccttta ttttcgacaa catcgatcca
    15721 acgttcagag aatgcttttt ctacgatggc catgtattct tcacccattg gtttcagtgc
    15781 ttctaaagct ttttcttttg cttcttcgta cgtaaaggta attggagctt cccctaagac
    15841 cggtgtataa agatcataca tgtgcagttt ttccacttct aataaacgtt tccgtaattc
    15901 catgtatcga tgtaacaaag gcaaatgctt gtttaccacg tctaccaaag tatcgtatac
    15961 actttcagga atatgattat tgctcaaaga cgcttctctg gcagagctgt aattacggac
    16021 tttcgcttta aaattatgtc cttttatatg tgtgcctaaa gtagaagcaa atgtatttct
    16081 aaattgttcg taaacactgt acaatccctt aaatgctgct tcgcgcaccc ttcgatccgt
    16141 gctttctagc aactgaccat acacgccatg agataattgg actatttcac cattttctcc
    16201 ttcaatcgtt ggaaaaacta gatctgcatt attcaaaaca gcaaatgtat cacttgaagc
    16261 atcaaagatt tcacctgctc cagcaagaag agattcctgc tcagcagata agacatgggc
    16321 tcgattatct actatttgct ggatataatg gcgatagact tccaattttg gttcttcttt
    16381 gaaatattgc caaatctggt catctgacaa ttgcaatatt tctggttcaa accaggaaac
    16441 ggcttcacta actttcgaaa acagactgct tgctcttgca taaagcgctt ggtaatctgt
    16501 atttccagtg tcttgatcgt ttttaaggtg cgcatataca taaatgactt cagtttggcg
    16561 ataaaccctc aatacgaatt caatcgcatt taaaaattga gaagcgcctt gatcaagtgt
    16621 tcctttgtgt ttttcagatt gttttaactc ttctgataat tccaaatatt tctcatcaaa
    16681 ctcttggtcg cttgaaaaga tcttggtcaa gtcccaagtt aaattttcag gcaattcttc
    16741 tcgttttggc aactgcttta cttccatgaa atccactcct tttcttccct tagctctatc
    16801 tatcaaaata gatcatagtc taacatccac atgtcacagc atctaacttt tctactcatt
    16861 catgtgtctg cttccatcaa ttttagcata actaaatctt gttttacata gaaaaccgat
    16921 tcctagccta tttttatact tactattttt tcaaagaaga caagtgtatg cattcgttaa
    16981 aaaactgctg atagaccatc tcttcatcaa tcataggaaa aagccattcc cttttatgtt
    17041 cttttatttg gcaaagccat gagttgaaat caggaacttt ctggttttcc aaggcttctt
    17101 cgtacaacat tcgataaaga aaaacctgtt cttgtaaata aaagaaaaac cgactttttt
    17161 gatagatcca cgatgataga tgcaaaagat gtttatgacg caagtagcat tgttcttgta
    17221 tggatactgt attcttttgc ttgtgaataa gttggcgtgt cagccatttc ttcccatctt
    17281 cactcacttt ttctattttt ttcatttggt tcattttaat ttcgttacta gtgaatattt
    17341 ctttaaggtc cctactagaa aaagtccagc agctactgca aaaggaaata tgccctgata
    17401 cagtctctgt aatatgatga tacaaataaa gcttttgttt cttgagatca gctttccagc
    17461 aatgcactcc tctttttcta ttatagtagc agcaactttt ttctatcaat gagaattgcg
    17521 attgtccaag aaaagaatta ccaagtatcc accatacttg gtaactgtta ttcaaataat
    17581 tttgggttct ttcaataaat ctctggtgag acagtctaga acattggatt tctattgcta
    17641 gattcgccaa caaaatatcc ggcctttgcc gcaacctcgg caaataggct tccaaaaata
    17701 ctggttctac tgactgtccc agccaatcca tgaagcattc ctttaactgt aagtgctctt
    17761 ttgattctct ttcactaaag accgaacagt tatctgattt ttgatgggca aaatgagcac
    17821 attttttctt tccctttttc aaaatcactc tttttcgaca gccacgacaa taatatttct
    17881 ttcctttttc tgcatgttta gcaaaacagc acttattata ttcattcatt gcaagcagca
    17941 tttttctccc tctgaactta tttacgtcta agaatttatt tacacgaaaa aagagggcgt
    18001 gaaaaagcta ttctgcatca agtaataaga acagctaaac aaaaatagtt cccaatattt
    18061 ttcgtttggt tgggcttgtt accgcagatg caagcttttg aacacattta tacgaaaaaa
    18121 agactgcgac acaatttttg tcacagtctc ttactataat gctgatttat acaaaataaa
    18181 atttcgttcg ctcgatagca ttacgcgcca tcagacattg tccatattca ttcaatgttt
    18241 ctggtgtacg atcgctcttt tcagcaaact ccaaaattcg agcaatctga ttctctacat
    18301 ccgcttggtt gagattttcc atccagaagt gaacgactag gtaatagcgc tcttcgaata
    18361 aatacaaatc agtccaagca gactctagtt cgacatctgt tgcaagttgg atcacttgtt
    18421 caaaatcttc aaaagcaaaa gttgcttgtc tttcctgttc ttctagggcg cttccagata
    18481 attcttcctc cggatcgctg ccaataatct gctgacgaag aaagtcactc acatcttctg
    18541 aatcaacagt agaatcttct gattgagaaa tttcttcatc cggaagattt ttactgataa
    18601 acagttctaa tccgtctcct tttggcaaaa cttggaaagt gacagcttcg cttcctcgga
    18661 actcctcttc gatatcaact tcttccaaaa tactataaaa gaaattttcg atttctttgt
    18721 gattgccaag taaatccaaa aatgaaacgc cccgttcctc taaatcttca tgaccaatta
    18781 gtacacggat cgtattttca ttaatatgtt ccatttccat tttgctacac ctcactttgc
    18841 cacaatgttt aatatagatt cattgtagcg aaatcagctt aaatgtaaag aaaaaaaatc
    18901 tcgctgttaa gcgaatttaa taaaaccagc ggatttttca acctttcaag ctatcttatt
    18961 taactataac atttttggca gacgaatact atatagccga cttttcagct aattttccct
    19021 ctctattttt cgtgcaatca tcatcttcta ctaccttttt atacatctaa tcaagaatac
    19081 tttttgtaaa aacaaaaaaa cttggaaaaa ctatctttgt aatagt
//
LOCUS       DARBDV010000060        18740 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000060 DARBDV010000000
VERSION     DARBDV010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.060"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            256..531
                     /locus_tag="SCM56_001898"
     CDS             256..531
                     /locus_tag="SCM56_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110661.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            544..2616
                     /locus_tag="SCM56_001899"
     CDS             544..2616
                     /locus_tag="SCM56_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEG1110662.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2781..4667
                     /locus_tag="SCM56_001900"
     CDS             2781..4667
                     /locus_tag="SCM56_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEG1110663.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4679..5626
                     /locus_tag="SCM56_001901"
     CDS             4679..5626
                     /locus_tag="SCM56_001901"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEG1110664.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5646..6173
                     /locus_tag="SCM56_001902"
     CDS             5646..6173
                     /locus_tag="SCM56_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEG1110665.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6236..6889)
                     /locus_tag="SCM56_001903"
     CDS             complement(6236..6889)
                     /locus_tag="SCM56_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEG1110666.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7021..7863
                     /locus_tag="SCM56_001904"
     CDS             7021..7863
                     /locus_tag="SCM56_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEG1110667.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8021..8917
                     /locus_tag="SCM56_001905"
     CDS             8021..8917
                     /locus_tag="SCM56_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEG1110668.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8920..9633
                     /locus_tag="SCM56_001906"
     CDS             8920..9633
                     /locus_tag="SCM56_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HEG1110669.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9653..10171
                     /gene="msrA"
                     /locus_tag="SCM56_001907"
     CDS             9653..10171
                     /gene="msrA"
                     /locus_tag="SCM56_001907"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEG1110670.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10168..10395
                     /locus_tag="SCM56_001908"
     CDS             10168..10395
                     /locus_tag="SCM56_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEG1110671.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10547..11098
                     /gene="lepB"
                     /locus_tag="SCM56_001909"
     CDS             10547..11098
                     /gene="lepB"
                     /locus_tag="SCM56_001909"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEG1110672.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11181..11681)
                     /locus_tag="SCM56_001910"
     CDS             complement(11181..11681)
                     /locus_tag="SCM56_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEG1110673.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(11960..12358)
                     /locus_tag="SCM56_001911"
     CDS             complement(11960..12358)
                     /locus_tag="SCM56_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG1110674.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12618..13478
                     /gene="ylqF"
                     /locus_tag="SCM56_001912"
     CDS             12618..13478
                     /gene="ylqF"
                     /locus_tag="SCM56_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEG1110675.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13471..14238
                     /locus_tag="SCM56_001913"
     CDS             13471..14238
                     /locus_tag="SCM56_001913"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEG1110676.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14294..15151
                     /gene="dprA"
                     /locus_tag="SCM56_001914"
     CDS             14294..15151
                     /gene="dprA"
                     /locus_tag="SCM56_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEG1110677.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15273..17351
                     /gene="topA"
                     /locus_tag="SCM56_001915"
     CDS             15273..17351
                     /gene="topA"
                     /locus_tag="SCM56_001915"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEG1110678.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17320..18696
                     /gene="trmFO"
                     /locus_tag="SCM56_001916"
     CDS             17320..18696
                     /gene="trmFO"
                     /locus_tag="SCM56_001916"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HEG1110679.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
BASE COUNT         6518 a         3231 c         3899 g         5092 t
ORIGIN      
        1 ttttgtcgca gccccatttt tttgttgtga taaaagaaca agaaaggcag gaatgggaaa
       61 gtaaacatcc ttttgtcaga aacttttatc tttcttaagt tttacttgat atctgatact
      121 atttggaata gaatgaggcg ttgaaaaatt tatggaaatt tctaactagc aataaacgat
      181 gaaacaatga aatatttttc atccaaaaag aactttgcta taatgatgga agtttgtgta
      241 taggagatct ggaaaatgag agagagtttt gaacaacaaa aaaaactgtt gcatgatcgc
      301 tatggcgccc tttcgatgga tgaccgccgt caaatacttt gtaagttgcg taaaagaaat
      361 atattgatgt atcgtcagtt ggaacgtctg aaacatgatc tgcttcgttt ggagtcaaaa
      421 cgtgtacagt gtgaattaga ggggaaccaa acacaggttg aagtagtcga aaccaaaatt
      481 ttaaagaaaa aagaacaatt tttaaaaatg cttacacaaa ataaaaaata aaggggatgg
      541 acgatggaat ttgctgagtt agttattgta tttgcagtaa cgatcacctt atcgaacata
      601 gctagccgca ttttgccaat gattccagct ccactgatcc aaattttcct tggagtggta
      661 ttaggtctga cagagtgggg acaatcaatc gattttgaac ctgaattgtt cttagttatg
      721 attattgcac cattgttatt tagagaagga gaaaaagcag atatttcttc gattctcaaa
      781 aatttcggga caattttgtt cttggcattt ggaggagtca ttttgaccct tgtcggggta
      841 ggtgcgacgt tatcattcct gttgccaagt gtcccacttg ctgcctgctt tgcttttgga
      901 gcagcgttag gtccgacaga tgcggtagca gtcagttctc tttctggacg agtgaatatc
      961 cctaaaaagg cgatgcatat tttggaagga gaaggtcttt tgaatgatgc atctggtgtt
     1021 actgccttcc agtttgctct tggtgcgctg atcaccggat ctttttcagc agtaaacgcg
     1081 gggatgagtc tagtagtttc tagtattggg ggagctttga tcggcttttt gcttgtctgg
     1141 ttcaaacaaa aaataattca tttgatcgaa aaagcatcag cacaagatgt cacagggtat
     1201 ctgctgatcg aacttttgct tccttttcta gcctatgtat tggcagaggc atttgatgta
     1261 tctggaatca ttgctgcagt ggcagcaggt attctccaag cttctggttt taggaaaatc
     1321 tctgtgtttg atgcagaatt gtcgagcttg tcccacagta catggacaac tattgctttt
     1381 acactgaatg cattagtatt tatattttta ggaatcgaat tgacgcaagt gttctcgcca
     1441 gtatggggag acggtttata tccaaacggt cttttattag cgattatcgt attaatctct
     1501 gtcatgcttt ttgtgatcag attcatttct atcagtcttt tttatgtatt caaagatggc
     1561 agtaaaaagt tcaaaaaaca gttgaacgag atcttgatct taacatttgg cggtgtaaag
     1621 ggaacagtca gtttggctac gatttttatt ttgccatttt caatcaataa tatgatgttt
     1681 taccagcggt ctttgctttt gtttctgacg gcgggtgtca tcttagtgac tttggtcata
     1741 gggatcattg tcttgcctat gctgacggaa acagaggaag cgaaaagcac ggatttgaat
     1801 gctttgatga ttttagaaga ggtcgttgaa gttttgcgaa aagaaataaa ggaaattgat
     1861 cgtaatacaa aagaattttt agccactgaa gcagtgatag aaaattatca agaacggata
     1921 cgtgatctgt acttggaaga tttaaccgat gatgaaaagc aagaagtaca agaaatacaa
     1981 gcactgattt tgtcgatcga acgggatgga ttggatgaaa gttatcgttc gggcaagctt
     2041 tcttctaatg gttatcgttt ttactcccgt tttctttctc gattcgaaca ttctatcaca
     2101 agccagattt tgtcttttat cggcttttgg ttcattgttg tccgtaggtt aatgcgaatc
     2161 gttctccatc cgaaaatgtt ttggcaaaga agatatgctg atcgtcagac atttattagt
     2221 gatgaagata tccaagaaat cagagagaca tatcaaaaaa atactcagtt gattattgaa
     2281 agtttagata acttgactga tatttatgat gaaacgatgg ttcaattttt tatccagcaa
     2341 cgcaaacttg aaggaataaa aatgaactcg gggaatttga tttcttcgtg gatgatccag
     2401 caagatagtc tgtttacgaa aaaaatgcta agaggttatt atcttgaaag aaaaatcatt
     2461 gatgaatacg aagttgctga aaaaatcacg acgttttctg cgaacagcta tcgcaggaat
     2521 atcaatttgt tagaatctta tacgatgaac aaaccatctg ataacttttc tttccgattt
     2581 gcgtttcgag caaagtcaaa aaaacgagtc caataaaata aaaaggtaaa aattttcaag
     2641 ttttataaaa aacaaaacat atcagaacag gatcgaatcg gtcctgttct ttttttgaga
     2701 acacaaaaag aacaaatacc aaaaaaattt tcctgaagtt cactattttt atgatacaat
     2761 atccaagata aggagggaac atgttgaaag aattaaaagt aactgacctc aaaaaaactt
     2821 atggtgaaaa agatttgttt gaccagattt cttttttggt ccacgaaaaa gaccgtatcg
     2881 ggttgattgg gacgaacggg acgggtaaaa caagtctctt aaatattatt gccggtatcg
     2941 atagcagcga cggtgatcgg caaacagtgt tttatccaac tgattaccgt attggttatc
     3001 tttctcaagc agctgaattt tccgatgaat taactgttct ccaagcagtc tttcaaggaa
     3061 acagcccctt gatccaaact gttcgagcat atgaggaagc attgatcgaa ctaggggaaa
     3121 acggagaaga ccctgatgtg caaaaacgtt atgctaaagc agaagaacaa atgaataaag
     3181 aagatgcttg gactacagat accaacgcaa agattatttt gcaaaaatta ggaatcagtg
     3241 aattagataa aaaaatcagt actttatcag gtggtcagaa aaaacgagtt agtctggcgc
     3301 aagtgttgat tgatgaaccc gatttactat tactagacga accaacaaac catttagatt
     3361 atgaagcgat cgagtggttg gaaaactatt tgaaacaata ccgtggcgca ctattgatgg
     3421 ttacccacga tcgttatttt ctagatagag tagcaaaccg aatcttcgaa ctttcatttg
     3481 gaaaattata cgagtataaa ggaaattatg aagcttacgt cttagaaaaa gcagaacgtg
     3541 atcgtgtaga agtagaacaa gaagaaaagc gcaagcggct ttataaacag gaattagctt
     3601 ggatgagggc tggcgtacag gcacgtggca ccaagcagca agcacggatc aaccgtttcg
     3661 aagatctaaa ggagaacttg taccaagtaa accaagaaga tgatttagag ctgaacctag
     3721 cgacacaacg tttagggaaa aaagtactgg aaatcaaaga tggcagttac cgtataaatg
     3781 accaaacact gctagaacat ttagatttat tgattcaatc tcgggaaaga ctaggtatta
     3841 ctggtaaaaa tggtgcggga aaatcgacat tgcttaatat tctagctggc agaattcccc
     3901 tggatagcgg tacaatgtct atcggtgaaa cggttcatct tgcgtattat acacaagaaa
     3961 atgaagaaat ggcaccagat aaacggatga ttgcttactt acaagaagct gctgaagaag
     4021 caaaaacagc agatggcagt caaatcggag tagcagaatt acttgaacga tttttatttc
     4081 caagatttat gcatgggaca ctgataggca aactttcagg cggagaaaaa cgccggttat
     4141 ttttgttgaa actgctgatt cagcaaccta atgttttatt attggatgaa ccgaccaatg
     4201 atttggacat agcaacattg acgattttgg aagattattt tagaagtttt ccaggtgctg
     4261 tgatcactgt ttcccatgac cgttatttct tggacaaggt agcagataag ttattagtat
     4321 tccaaggaaa cggaaaacaa gaactttatt acggaaacat gtccagttat ttactgaagc
     4381 aaaaagagac ccagcagcct gctgaaaaag cgaaaccaaa aatacagagc aaggaacctg
     4441 ctgaaaagaa gaaattatct tacatggaac aaaaagaatg ggaaacgatt gaagatgaga
     4501 ttgcagaact tgaagaaaaa atctcattgc tccaagaaga gatgaatcat caaggagata
     4561 atttcactcg attacaagaa ttgcaaaacg acgtatctga aacagaagca caacttgaag
     4621 aaaaaatggc acggtgggaa tacttgagtg aatgggtaga agattaggag gaaaaaggat
     4681 ggaacaagcg tatcttgact taggaagaaa attgttggaa gaagggcatc gtaaagaaga
     4741 cagaacaggc acaggcacat taagcttatt cggataccaa atgcggtttg atctgcaaaa
     4801 agggtttcct ttgttaacaa cgaaacgagt accttttgga ttgatcaaaa gcgaattgct
     4861 ttggttttta aaaggagata cgaatatccg ctatctatta caaaataaaa accatatttg
     4921 ggatgaatgg gcatttgaac gctacatcaa aagtccagac tataatggac cagatatgac
     4981 tgattttggc catagatgtt tgacagatcc tgaattcaat gaagaatata aaaagcagag
     5041 caagcttttt tgcgagaaga tcctaaccga tgattccttt gctgaaacat acggcgatct
     5101 tggacacatc tacggctatc aatggcgtca ctgggaaaca aaagacggtg gctttatcga
     5161 tcaaatcaaa caagtgatcg aagcaatcaa aaagactcct gattctagaa gattgatcgt
     5221 ttctgcatgg aatccggaag atgttcctag catggctttg cctccttgtc atacgatgtt
     5281 tcaattttat gttcaagagg gacggctaag ctgccaattg tatcaacgca gtggggatgt
     5341 gtttctaggc gttccgttca acattgcaag ttatgcgttg ctgactcacc taattgcaca
     5401 tgaaacaggt ttagcggttg gcgaatttgt tcacacatta ggagatgctc atctttatat
     5461 caatcatatc gaacaaatga aagaacaatt gagccgagaa atacgcgcat ttccgacttt
     5521 agttttgaat caggaaaagc agtcagtatt tgattttgag atggaagata tccaattaga
     5581 aggatatgac ccacatccgg cgatcaaagc tcctatcgct gtttagaatc agaaaggatg
     5641 agaaaatgtt agctgcaatt tgggcacagg atgaaaacgg attgattgga aaagaagacc
     5701 gtcttccttg gcgtttgcca aatgatctga aattcttcaa acaaatgaca gaagcaaata
     5761 cactagtgat gggacgaaaa acattcgaag ggatgggtag ccgtccgttg ccgaatcgtc
     5821 agacgattgt tttgacgaga gattcttcct ataaagcaga aggcgtgcat gtcatgcata
     5881 gtgtggaaga cgtgcttgct ctggaaaaag aaacagacgg tatctttttc atcgctggag
     5941 ggtcagctgt ttatcaagaa tttctgccat tttgtacgat attataccgt acagtcatcc
     6001 atcatgtatt cgatggagat gcttattttc cacctgttga ctggtctgat tggtcactga
     6061 tcaatactag tcaaggagaa atggatgaaa aaaatcgtta tccgcatcaa tttgaaacgt
     6121 atcagcgtaa tgagaatcct gtaaaaagcg ggacaggcga attgacgata taaaaaagct
     6181 atggatagac tagctgttat ttagagctgt cccatccata gctttatttt ttttattaag
     6241 taaagaataa aacagagaag tacataaaac ctgcacctaa cataacaaac aaatgccaaa
     6301 caacatgcat atagcggata gaattcaagc tataaaagaa tgcgcctatc gtgtaagaaa
     6361 gcccgcctgc taccatcaaa ataacaccag tcattccaag agagtgataa agaggaacag
     6421 cagccacgac acagagccac cccataaaaa tatagatgat cgtagatact tttgatactc
     6481 tctcccgttt atgcaacgtc aacgctttat atataatgcc aagaatcgca aacaaccaga
     6541 ttaatacaaa taaagtccaa cctagccatc ctcggatact cagcaaacaa aaaggagtat
     6601 aacttcctgc aatcaaaaga aaaatcgaac tatgatcgaa tacttggaac accttttttg
     6661 cttttgtaaa aatcaatgaa tgaaacaaag tagacgttaa aaataaaaga atcatcatcg
     6721 atccatagat agcataagag acaatatgga gggaggaatg caagtgggca ccttttacta
     6781 aaagaaccac tagtcccgcg atactcaatc cagtcccaat tccatgggtg actgcattaa
     6841 acacttcatt taatatttca tacgttcgat taaatttcgt tttttccatg aaaacggctc
     6901 ctttactagg tgtaattaat gaaaaatcat tatttgtctg ttatactaaa aatagaaatt
     6961 gtcttacatg tagtttaaca tgaataaggc atcttgttaa agaaagagag agagaaaata
     7021 atgacaaatg taaaaatcgt aacagattct tcatgtacga tggaaaaaaa tgtacgcgat
     7081 gctttaggca ttcgtgtcat gccgctatcc atcatgatcg acggaacagt atatcccgat
     7141 gatgaccaac tagacggcga aagatttatg gaaatgatgg cgaaagcaaa aaatctgccc
     7201 aaaacaagcc aaccaccaat tggagaattc atctcgatgt atgatgagct aggagaagac
     7261 ggcagtgaag tgatttcgat ccatatgaca aaaggcttga gtggaacagt agaagcagca
     7321 agacaagcca gtaatctaac aaaaacgaaa gtaacggtta tcgacagtga taccactgac
     7381 caaggattat cttttcaggt aattcgagca gctgaattag caaaagaagg taaaacagta
     7441 gagaaaatcc ttgaagaagt caaaaaagta caagacaata caaaattata catcggaata
     7501 tccacactag ataacttggt aaaaggtgga agaatcagcc gaacaactgg tttgctgtct
     7561 agtgttttga acatacgcgt ggtgatgaat ttcgcccatt ctgaattaat ccctgtaact
     7621 aaggggcgag gaaagaaaac gttcgataaa tggtttaata atttgaaaaa tgaactgaaa
     7681 gaattgccaa atgtgcgaca aatcggtatt tcacatgcgg atgcaaaaga attggcgcaa
     7741 gaattcaaag agggtttgca agaattattc ccggacatgc atatccctgt actgcatacg
     7801 aatccggtta tcgctacgca tactggtaaa gacgcgtttg caatcatgta ttatgtagat
     7861 tagtcgaaaa gaggataagc cctcagcctg aaaggcttgc agcttattct ctttttcttt
     7921 ataaaattag gaaaataatt gttttctttt ttcataaaga ctaccttttt gataaaattt
     7981 ttgataacat tagaaccaat cgaatacaag aggtgaaaaa atgaaattaa cgaaacgaca
     8041 tttgatcctc gttttgttcc cattattaat tgcgatcatc atctatgcag gtttagctgc
     8101 ttctataccg aaagctggcc cactaatgaa accagtaact actgtgagag aagatccaaa
     8161 taaaaaagaa aaacttcact acatagctat cggagattcg ctgactgaag gagtaggaga
     8221 tcaaaccaag caaggaggat tcgttcccct tgtcgcaaat gatctaaaag accgttataa
     8281 tttgaccgcg gttgaaacag aaaactatgg tgtaaatggt gaacgaagtg accagatctt
     8341 aaagcgagta aaaacaaaag aagaaattcg aaacaatatc aaaacagctg attttatcac
     8401 actgactgtt ggcggtaatg atttgatgaa agttatccaa aataatttat tcgggttaac
     8461 ggtcaattcc tttaaaaagc cactgaaaaa ctatcaggaa aatgtcacag aactattgga
     8521 agaatttcga tccctcaata aaaccgcacc tatttacatt ttaggcatct acaatccatt
     8581 ttatttgaac tttcctgaga tcactgacat gcagacaatt gttaacaatt ggaacaaagg
     8641 aacagaaacg gtgacaaaaa aaatgccgaa ctgctacttt atcccaatca atgatttgct
     8701 ttatcaagga ttgaatcagg aagtaggtgt cacagaagaa ggaacagatc aaacaactag
     8761 cacgacagaa aatctaaatg gcatcaaaaa taatgtgctc tatgaagaag atcatttcca
     8821 tccaaataat ttaggttatc aattgatggc aaatgcagtg agagatcagc tgatcgagac
     8881 acaaaaccaa tggttagaga aggaggatga tcaataaaca tgaaaacaag atctgacgta
     8941 tcttcaaaca aaacaaagaa aacaaattac tggaaattcg ccttccttat ccttcttggg
     9001 gtagtacttg gttcgaccgt atttttaggc agccgtattt ttgctaatcg ggaaccagaa
     9061 ctcccagaaa taccagctct tacggaaaga caaggagatc ctgtcttaac gatcaattca
     9121 aataaagaaa aagtcaatca aatcatcagc tttttcctta gtgagtatca aaaagatagt
     9181 gatattgaat ataaatttta tttggaaaat gaagcactac tcaatggtac tttcgaagta
     9241 ctaggttttc ctatcgattt ctatttatat tttgatccgt atgtgatgga aaacggaaat
     9301 gtccaactaa aagctagaag tctttctatc ggaacattga gtttgccaat caaagatgtg
     9361 atgaatatga tcaagagaaa ctataaatta ccagattgga tcgaagtcaa tacagatgat
     9421 ctgactatca tgcttaggtt ggatcaattc cgaatgcaaa acgggatgta tattaaagca
     9481 gataaaatcg acttagtaaa tgatgacatt cggtttagtc tatatttgcc aaaagatacc
     9541 tctgatgctt caaacaactc aaataccaac aaaactagca ccagcagtac tgaaagcaag
     9601 acaaaagaaa ataaaaaatc atcggatgag tagtattaga gggggaatca taatggaacg
     9661 agcaatattt gcaggaggct gcttctggtg tatgatccag cctttcgaca cgcttcctgg
     9721 tatccataca gtgatgtcag gatatactgg agggcatgta ccagatccga cttatgaaca
     9781 agtaaaaagc caaacgactg gacatacaga agcagtggag attatctatg atccaacatt
     9841 gatttcatat gaacaattag tagaacttta ctggcagcaa actgatccaa ctgacgcttt
     9901 cggacaattc gaggatcgcg gtgacaatta tcgcccggtg atcttttatt tgacagaaga
     9961 acagcggatc attgctgaaa aaagtaagaa acagttagct gaaagtggac gtttcgaaca
    10021 gccaatcgtt acaaaaatcg aaccagccca accattttat ctagcagaag aagaccatca
    10081 agagtattat aaaaagcatc ctgaaaattt taaaagaaac catgcacgcc gggctgcctt
    10141 tatcgaagaa aattggagga acgaagggtg aatcgaagct tttttcatta tttaatgaca
    10201 ctgcgaggcg gtggcacaca tgatgcttta tctgttttcg ctacagaagc cggtaaagac
    10261 atccaatttc ctaagcacag ctctagctac gaagaaattt ctaattatct ggaactgaat
    10321 gtcgactatt tgccgtccat ggatatcttt gacgaagcat gggaaaaata tttagaaaat
    10381 aatcatgaca tataagagat agttttgtac tttatgatca aaaaagtttt ttgtccaata
    10441 gcctaattga aagaattatt tttatttcag ctatctagtc gcattttttt gatatttttc
    10501 ttataataaa gaaagaatga aaaaaagagg gatggattga tagagattga caaaaaaaca
    10561 acgctatatt aatcgcttct ggcttgtatt caaatactta cttgtttctg tattccttgc
    10621 gttcatgttg cggggatttt tattcatccc ggtaccagtg gaaggcaatt caatggaaaa
    10681 cgtgttgaag caaggtgata tggttgtgat ggaaaaattt tcggagatca gacgatttga
    10741 tattgttgtt tttcaattag cagatggcac gatttatatc aaacgagtaa tcggtcttcc
    10801 aggagaaaat gtcagctacc aaaatgatca attgaagatc aatgggaaag tagttaagga
    10861 accttatcta actaaaaacc tgaaatcaga tcatgcaaat gcatcctata cgacggactt
    10921 tacactacaa gagctaacgg gacaatccaa gcttccggaa gataattatt ttgtacttgg
    10981 agataatcga agagtttcaa aagatagtcg ttcgtttggc acgatcaata aaactgatat
    11041 tctaggtaaa gcaagatttg tctattatcc acttgatgaa ataaaatgga tccagtgaga
    11101 aacaataaag cgtgcagtct gttgagactg cacgctttat tgtttaatcc aaaatcagtt
    11161 tactacctta gtacgtaaaa tcaaacagct cgtttaaaca cttttctagc ctctaaagtt
    11221 ttgataaatg aataaacaac cattatttga atagggattt ggatcatatt cctgatgaat
    11281 ctacctagaa aaattacttt gaaatctgca ccaaacaaaa cagacatcag ccaagaattg
    11341 ataaaaaggc tgactgggat cataatcaat aattgggcag cgatgacgga aggcaggttt
    11401 atctttttat gatataaaaa taagccatta ataaatccac caaggaaagc cgtgataaga
    11461 aaagcagctt gaaacgttcc tgaaccaaat atcgtgaaat ttaaagtgtt cgacaacatt
    11521 tcaagcaaac cagcccagat cggtccatat agataaccca taagcgccat acttaaaaat
    11581 ccgaatgtga tttgaaaata agcaggacca atcgctatct tgtataagat gacatcaagg
    11641 gctaataaaa gagcagaggt cgacaacaat cttgcattca tttcgggcaa tctaaaaacg
    11701 tcttttgcaa tcaaagtgtt caaaatattc tctcctttgt cagttaataa taaaaggata
    11761 ccataaaatc accaatgtgt catgacacat tggtgattta catacactgg catctaacaa
    11821 ttaaaaaaag ggtgagaaga ctgcttttat aaactgtaaa aaagagattg tacgaataaa
    11881 aaaaatagag gcggagaata cttatcgtat catccgcctt tgtttttttg ttataaaaaa
    11941 cgtcagatta gttatctgct tacatcttca tccatacggg ttctaatgta tttccgtcta
    12001 gatcttggac ttctaatgaa aacatttctt cttcaggcat tcccatatct acttcgtgga
    12061 aagtaccacc attttctttt gcaatttgag caaatttttt tacttcatcc gcgttttcaa
    12121 cactaaacga aatcaaagca ccacttgttt ttttattatc agctatttct ttatctttta
    12181 taaatttttt ataaaaatca tgagagagaa gcattatcca aaaagtgtca tcccatacca
    12241 ttgaaccagc ttcttcagta gaaaattctt catttttttt aaaacccagc ttttcataaa
    12301 aagcaacgga acgattgaca tcagcaacag gaaaattaac aaaaaccata ctattcattt
    12361 gtttcgtctc ctttctgttt tgttcgtcta tcatctctta tttaggaaac gctgtcaaaa
    12421 atttagattc atatatagaa accaaaataa aaatttgcat catcaaaaaa gtgaagatgt
    12481 gatagaatga ggactggttc actttcactt aactaataga tggaatttct ttataataaa
    12541 aaagtgaatt tatattgaaa aagaaaaaaa caagtaaagc cggggaatat tccggtatga
    12601 aaggaatgga gagaacaatg accatccaat ggtttccggg acatatggca aaagcccgac
    12661 gagaagtttc tgaaaaaatg aaatatgtgg acatcgtatt tgaattggta gacgcacgat
    12721 tacctttatc ttctcgtaat ccaatgctag atcagatcat tcagcaaaaa cctcgccttg
    12781 ttttattgaa taaagcggat ctggcagatc cacaacagac aaagctgtgg caagaatatt
    12841 ttcgtaaaca aggccatctt gcattagcta tcacggcaca agaaagtaaa ggaattaaag
    12901 gtctagtacc tgcggctaaa gaagcattga aagaaaaacg ggcaagagat gctgctcggg
    12961 ggatcaagcc tcgcgctatt cgggcaatgg tcttaggtat tccaaatgtt gggaaatcta
    13021 cgttgatgaa tcgactagta ggcaagaaaa ttgcacagac aggaaataag cctggcgtaa
    13081 caaaaggtca gcagtggctt cgtttaggta atgagttgga attgttagac acaccaggga
    13141 ttttatggcc caaatttgaa gatgaagaaa tcggcaaaaa actagcgctg acaggcgcga
    13201 tcaaagacca gcttgtccat ttagatgatt tagcgattta cggattagac ttttttgccc
    13261 gatattattc aggaagaatc agcgatcgtt atggtttatc aatcgagcaa gaagaaaaat
    13321 tggcaccgga attactgatg gatattacag caaaacgtgg gtataaagaa gactatgatc
    13381 gtggcagcga attagtcgtg catgatattc gtcaaggaaa acttggacga tacacactgg
    13441 atcgttgcga agaaatggag caaaaaagtg atgactgaat ccatccaaag tatcaaagaa
    13501 cgattgaaaa gcatacagag aatagatgat cctacactag aatcattaag aaatgatcca
    13561 cgcaaaggcg tgcagcaagc actggcttct tttgaacgac gattgaaaaa ggaagcatta
    13621 attaaggaaa aatatgaaga aatgttttgc tttgaaaagc aagggtacgc acaaggtcac
    13681 caatggattg ccggcattga tgaagtagga agaggaccat tagctggacc tgtcgtggca
    13741 gctgcagtta tcttgccgaa aaatgagatc atattagggc tgaatgactc caaacaatta
    13801 tcagagaaaa agcgagaact tctttatcaa caagttcaag aaaaagccct ggcaatcggg
    13861 atcgggatcg ttgatcagca gacaatcgat gatgtgaata tttatgaagc aagtaaatta
    13921 gcgatgatcc aagcagtaga aaaattgtcc attgtaccaa gctatttgtt gatcgatgcg
    13981 atgaccttgg atttgcctat tcagcaggaa aaaataatca aaggggatgc acgatctgtg
    14041 tcgattgctg ctgcaagtat cattgctaaa gtatatcgag accaattgat gaaagaatat
    14101 gacgtgcttt atcctggata tggtttctcg cataatgcag gttatggaac aaaggaacac
    14161 ttagaaggac ttgaacgaca aggaattacg ccaatccatc gtaaaagttt tgcacctgta
    14221 aaaaaatatt tacaataaaa aacagccgct tttacgtatc ttattagaaa gagagacgaa
    14281 aggcggtttt atgatgtatc aaatagaaga aaatttattg aaacttgctt atacaaaagg
    14341 aatcagtagt caaggaaaat gggcacttgc taactggatg atgcagtatc cttataaaga
    14401 aatcggattt gaagacatca ttaagatagg taaaataacc aaacatcggg agctgtttcg
    14461 caaaagttgg caggaaattc acgctaactg gggcaaaatc caatcgaaac agtcgtttat
    14521 cacttgcttt gatcccaaat atccccctca gctgcttcac ttgacctatc cgccaattgt
    14581 actgttttat aatggtgatc tatctttact ttcttgtaac atgttaagcg ttgttggtgg
    14641 aagacacact tctgctttag ccaaaaaaac agtttattat ttactaaaac cagtgataga
    14701 agctggatat gtgatcgtca gtggctgcgc gaaagggata gatacatacg gtcaccaagc
    14761 agcaatctct catggtggac ggacaatcgc agtcattgga acagggatag agcaagtcta
    14821 tcctgctgaa aatcgtgact tgcaagaggt gattgcaaat gatcatcttc ttttgagtga
    14881 atatcctcct caatccggcc cgaaaaagca ttgttttcca atgagaaatc ggattatcgc
    14941 agctttgtct aaaggaacat gtgtctttga agccaagaaa aaaagcggct ctctgataac
    15001 agcacagcaa gcattggatc ttggaagaac agttttttct gttccaggaa atattttaac
    15061 agaacattct accggatgtc atcatttgat ccaagacggt gcagtttgca cggtttcggg
    15121 tgaagatatc ttggcagaat ttaaagaata acaccatact tttcatttgc ttttcttgac
    15181 aaacgttttc tgaatggata tgattagcta cgatttgtat gaatcctctt ttaatagtag
    15241 gaacacgaca atggaaagga gtccattcag taatggcgta taaatattta gtgattgtag
    15301 aatcacctgc aaaagccaag acaattgaga aatatcttgg acgaaactat aaagtaatgg
    15361 ccagtgttgg ccatataaga gatttaccga aaagtaaaat gggtatcgat tttgaaaaca
    15421 attatgaacc ccattatatt tctatacgcg gaaaaggcga tgtcatcaaa agcctgaaag
    15481 ccgcagcaaa aaaagctcaa aaagtttact tggcaagtga cccggataga gaaggagaag
    15541 cgattgcttg gcatttagcg tacctacttg ggttggatct gaaagaaaaa aatcgggtgg
    15601 tcttcaatga aatcacaaaa gacgcagtca aagcagcttt taaggaacca agaacgatcg
    15661 atgtagattt agtagatgca cagcaagctc gtcgtacctt agacagaatc gttggttatt
    15721 cgatcagtcc tattctctgg cgtaaggtca agaaagggtt aagtgcagga cgtgtccaat
    15781 ctgtcgcttt aaaaattatt attgaccgtg aaaaagagat ccgagaattt gttccagaag
    15841 aatattggag catcgacggt aattttaaaa aagctcgcaa gaaattcaaa gcaaatttct
    15901 ggggaatcga cggtaagaaa aagaaattac cagatgcaca aagtgtaaaa gaagtcactg
    15961 ctagaattga tggaacagac ttcgaagtta aaaaagtaga aaagaaagag cgcaaaagaa
    16021 accctgcgaa tcctttcact acaagtagcc tgcaacaaga agctgcccgt aagttaaact
    16081 ttcgaacaag aaaaaccatg atggtagcac agcaattata tgaaggaatc actttgggta
    16141 aacaaggaac agtcggcttg attacataca tgcgtactga ttctacccgg attgccgata
    16201 cagcaaaagc agaggccgca gcatttattg aagatacgta tggaaaagaa ttttctagac
    16261 atgatcaccg aaagatcaaa aatgctcaag gcgcacaaga tgcccatgaa gctgtgcgtc
    16321 caacgagtgt gcttcgtaca ccagatgaac tcaaacaata tttagataaa gatcaattga
    16381 aactttatac attgatctgg tcacgttttg tggctagtca gatgacacca gctattttag
    16441 atacaatgaa agtgacatta gaacaaaatg gtgtcatctt cattgcgaac ggttcaaaaa
    16501 tcaaattcaa aggattcatg caagtgtatg tggaaggtag agatgacggc aaagaagaga
    16561 aagaaaatat tcttccagaa cttgaagaag gcgatgtagt ccaatctgtt gatattgaac
    16621 caaaacaaca ttttactcaa ccaccagcac gttttagtga ggctacgttg attcgtgcac
    16681 tagaagaaaa cggggtaggg cgaccttcca cttatgcacc aacacttgat acgatccaaa
    16741 gacgctacta tgtaaaattg acacaaaaaa gatttgagcc gactgaacta ggagaaattg
    16801 tcaattcatt aatctgtgaa ttcttccctc aaatcgtcga tatccatttc acagcagaaa
    16861 tggaaggaga tttggataaa attgaagaag gcacggaagc gtgggtcaaa gtggttgatc
    16921 gtttctataa accgtttgaa aaagaactga cgaatgcgga agaaaaaatc gaaaaaattc
    16981 aaatcaaaga tgaaccagca ggattcgatt gtgatgtctg tggacatccg atggtgataa
    17041 aattagggaa atatgggaag ttttatgctt gcagtaattt tcctgattgt cggaatacga
    17101 aaccaatcgt aaaagaaatc ggtgtgactt gcccagtatg tcatgaagga caagtcattg
    17161 aacgaaaatc taagaaaaac agactattct atggttgttc ccgtttccca gaatgtgaat
    17221 tcacttcttg ggataaacca gtgggcagaa attgtccaaa atgtgacgga tatctggtcg
    17281 agaagaaagt caaaggcggt aaacaagtag tctgtgtaaa tggagattac gaagaagaca
    17341 tccaaaaata atagaaaaga ggttctttca ttgaaaatgg taaatgttgt tggagcaggg
    17401 cttgccggaa gtgaagctgc ttggcagatc gcacaagcag gagtccctgt ccgtttatat
    17461 gaaatgcgtc cagtcaaatc gactgaggcg catcacacaa gtaattttgc tgaactagta
    17521 tgttccaatt cattaagagg aaacagtttg gctaatgcag tagggttatt gaaagaagaa
    17581 atgcgccgat tgaattcggt agtaatcact tcggcagatg aaacagcagt gccagctggt
    17641 ggtgccttag ctgttgaccg cgattctttt tctgaaacaa ttacccgaaa agtcaaagag
    17701 catccattag taacagtgat aaacgaagaa attactgaaa taccggaagg aatcaccgta
    17761 gtagcaacag gacctttgac ttctcatcca ttagcagaaa gcatcaaagc attcaatggt
    17821 tcagatggtt tttatttcta tgatgcagct gcaccaatcg tagacaaagc tacgatcgat
    17881 atggataaag tatatttgaa gtcccgctat gacaaaggag aagcagctta cttgaactgt
    17941 ccgatgacag aagaggaatt caaagctttc catgaggcac taatcaatgc cgaagtggca
    18001 gaacttaaat cttttgagaa agaaaaatat tttgaaggat gtatgccaat tgaagtaatg
    18061 gcacagcgag gattcaaaac gatgcttttc ggacctatga aaccagttgg attggaagat
    18121 cctaaaactg gcaaacgtcc ttatgctgtt atccagctgc gtcaagacaa tgctgcagct
    18181 tctttataca acatcgtggg ctttcagact cacttgaaat ggggcgagca aaaacgtgtt
    18241 ttccggatga ttcctgggtt agaaaatgct gaattcgttc gctatggtgt gatgcaccga
    18301 aacagcttta tgaattcgcc agaattgctg aaaccgactt accaatcaac gaaacgcgaa
    18361 gatctgttct ttgcaggtca gatgactggt gtagaaggct atgttgaaag tgcagcgagc
    18421 ggactacttg ctggaatcaa cgccgcacgc ttagcaaaag aaatggaacc agtcgagttt
    18481 ccacaagaaa cagctattgg aagtatggct tactacatta ctcatgcgga aggaaaacat
    18541 ttccaaccga tgaatgccaa ctatggtctg tttcctgaac tgccagagcg cattcgagac
    18601 aaaaaagcaa gatatgaagc aattgcacaa cgagctttaa aagctaatga ggcaataaaa
    18661 acagaattag aaaaaactgc aatcccaaat gagtaaggaa actccatgaa gagggtgaga
    18721 cggatagtct cgctcttttt
//
LOCUS       DARBDV010000061        18583 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000061 DARBDV010000000
VERSION     DARBDV010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.061"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            154..237
                     /locus_tag="SCM56_001917"
     CDS             154..237
                     /locus_tag="SCM56_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HEG1110680.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            362..1099
                     /gene="erm(B)"
                     /locus_tag="SCM56_001918"
     CDS             362..1099
                     /gene="erm(B)"
                     /locus_tag="SCM56_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEG1110681.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1362..2225)
                     /locus_tag="SCM56_001919"
     CDS             complement(1362..2225)
                     /locus_tag="SCM56_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEG1110682.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2227..2499)
                     /locus_tag="SCM56_001920"
     CDS             complement(2227..2499)
                     /locus_tag="SCM56_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEG1110683.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2516..2725)
                     /locus_tag="SCM56_001921"
     CDS             complement(2516..2725)
                     /locus_tag="SCM56_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HEG1110684.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(2824..3720)
                     /locus_tag="SCM56_001922"
     CDS             complement(2824..3720)
                     /locus_tag="SCM56_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEG1110685.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
     gene            complement(4018..4590)
                     /locus_tag="SCM56_001923"
     CDS             complement(4018..4590)
                     /locus_tag="SCM56_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEG1110686.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPISLGFYEQNWYLIGFAG"
     gene            complement(4866..5660)
                     /locus_tag="SCM56_001924"
                     /note="aph(3')-IIIa"
     CDS             complement(4866..5660)
                     /locus_tag="SCM56_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HEG1110687.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(5753..6295)
                     /gene="sat4"
                     /locus_tag="SCM56_001925"
     CDS             complement(5753..6295)
                     /gene="sat4"
                     /locus_tag="SCM56_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HEG1110688.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            complement(6292..7200)
                     /locus_tag="SCM56_001926"
     CDS             complement(6292..7200)
                     /locus_tag="SCM56_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HEG1110689.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            complement(7233..7967)
                     /locus_tag="SCM56_001927"
     CDS             complement(7233..7967)
                     /locus_tag="SCM56_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG1110690.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            complement(7948..8817)
                     /locus_tag="SCM56_001928"
     CDS             complement(7948..8817)
                     /locus_tag="SCM56_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEG1110691.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            complement(8832..9056)
                     /locus_tag="SCM56_001929"
     CDS             complement(8832..9056)
                     /locus_tag="SCM56_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1110692.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            complement(9199..10743)
                     /locus_tag="SCM56_001930"
     CDS             complement(9199..10743)
                     /locus_tag="SCM56_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1110693.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            complement(10763..11179)
                     /locus_tag="SCM56_001931"
     CDS             complement(10763..11179)
                     /locus_tag="SCM56_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HEG1110694.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            complement(11180..11677)
                     /locus_tag="SCM56_001932"
     CDS             complement(11180..11677)
                     /locus_tag="SCM56_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HEG1110695.1"
                     /translation="MLFLRDGEIKATLTTMMNKLAFSHKLILEPLFKSVSQIDEESDC
                     ERMDAIDKLMEQLLEERNTLIALMSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTN
                     SASGVLRANEIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLK
                     EKVVR"
     gene            complement(11988..12665)
                     /locus_tag="SCM56_001933"
                     /pseudo
     CDS             complement(11988..12665)
                     /locus_tag="SCM56_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(12665..13282)
                     /locus_tag="SCM56_001934"
     CDS             complement(12665..13282)
                     /locus_tag="SCM56_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1110696.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            complement(13296..13466)
                     /locus_tag="SCM56_001935"
     CDS             complement(13296..13466)
                     /locus_tag="SCM56_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110697.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            complement(13814..15304)
                     /locus_tag="SCM56_001936"
     CDS             complement(13814..15304)
                     /locus_tag="SCM56_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HEG1110698.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            complement(15347..15646)
                     /locus_tag="SCM56_001937"
     CDS             complement(15347..15646)
                     /locus_tag="SCM56_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110699.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     gene            complement(16012..16296)
                     /locus_tag="SCM56_001938"
     CDS             complement(16012..16296)
                     /locus_tag="SCM56_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1110700.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            complement(16815..17300)
                     /gene="vanZ"
                     /locus_tag="SCM56_001939"
     CDS             complement(16815..17300)
                     /gene="vanZ"
                     /locus_tag="SCM56_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HEG1110701.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(17453..18364)
                     /locus_tag="SCM56_001940"
     CDS             complement(17453..18364)
                     /locus_tag="SCM56_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HEG1110702.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         5332 a         3746 c         2840 g         6665 t
ORIGIN      
        1 gattcatcgt cattcctcct gctatctttt tctattattc taccttattt gatagtagat
       61 ttaaaacttt gcaacagaac caaaattttg tataatagga attgaagtta aattagatgc
      121 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgtaatgta
      181 gataaaacat ctaccgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      241 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      301 acaaacaaat cgtttaactt ctgtatttat ttatagatgt aatcacttca ggagtgatta
      361 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      421 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      481 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      541 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aacactcgtg tcactttaat
      601 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      661 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccatgcgtc
      721 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
      781 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
      841 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
      901 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
      961 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1021 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1081 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1141 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1201 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1261 aaattcctcg taggcgctcg ggaccccaac atgaggaaat gtttttataa aaaaagagta
     1321 gttaccgttt tttggtaact actcttttcc ttttttaagg tttaaatacc tggaagttta
     1381 ggtgttttgg gtattggtgg tgtaggtagc tgcaagcttt ccattttttg ttgaattgct
     1441 ttaaattcag gggtttcttg gtgttgattt tgaaccattt tttgctctat tctctccaaa
     1501 gtcggttgaa tttctttccc tgatacttta cgatttaatt ctctttctaa ggtctctttc
     1561 ggactaatgg aaggcgtttc taagcttgaa tagagtttca ctccttctct attgtaaagc
     1621 cttatatcgc taaataagcc cgttttatga agggtttcta aattggtcgg taagtttttg
     1681 acaacaatat catgcgcttg ttttggtgtt gccctagctg tcattggatc atctgcatac
     1741 atggtttcat atcgttcaat cgttcctaag tatgattcaa ttttcggtac tgccatgacg
     1801 tatatttttg tttcataacc tttagattga agcattgtgg ctgtttgaat gggaacgtct
     1861 gttgttcgtc ctgttccttc aatcactaaa ttatagcctt gatcgcttaa acggctaata
     1921 agtgcttctg tcatgcgatt cgaataaggg gtaacgtgtt ttactacgtc tttttcatag
     1981 agtttcacta attcgtcaaa atttgggtgc tgttgtttaa aggtatcatt atcaatgaca
     2041 ataacattcc cttgtgtttc ttcaaaaatt gctgatcgca aactggtttt ccctgaccct
     2101 ggttgcccac caagtaaaaa agcggttggc gattcaaccg ctagtctatt tttagttagt
     2161 cgttcaacat tcttttctaa acgatcttca aattgctttt cggtgaagtc agtaatattc
     2221 gccatgttaa gccactttct ctttattcaa aatttgtttt gaatattgat tcattgaccg
     2281 ccaatactca tgaatagctt gtaattcttc aaatggttgt tgaaataaat caacttcttg
     2341 tgccacttct aattgattta aaagattcac ttctagtaaa cgagtatttt tagtatctag
     2401 ttcatgattc aaaacataat ttaatacccg attataaaca cttgccgata actcattaat
     2461 gatttcaatt tcaaatgttt tttcatacgt aactgccatt tcattttcac tccttttaaa
     2521 gtttgtcagg taaatatttc cgtatatatt cttctaaggc ttgatccata acttctttta
     2581 cgtttccgcc attctttgct gtttcgattt ttatgatatg gtgcaagtcg gcacgaacac
     2641 gaaccgtctt atcccccatt atatcttttt ttgcgctgat tggcgtatca tttcgttttg
     2701 ctttctgtgc gcctaaattt cccacgatcg ctcacttctt tctatttctt cttactattt
     2761 attttatcat cacctatcac aaatcacaag tgatttgtga ttaatcactt gtgatttgtg
     2821 attctattct ttttcatttt ctaattgaat gattcgctca atcatttcaa aaaatacgtt
     2881 ctcatacata gacaaaactt ttttgtcata gcctttgtgt tctgtaatgc catttttaga
     2941 ccaagtactt actttattac ttcgcttgat aatatttcgg aaaaccaaat tatcctcttt
     3001 atgttctttg tacagttctt ccaggtttga ttttatcgtt gcgctgtccg tatcaactaa
     3061 ataaggaaca aaaccaatca tatctagtcc agggttaaac tgttcttgca aatcaatcaa
     3121 ataggaaata tagttttgaa tgttgtttgt actttcttct tctgcttgta aagggatcat
     3181 aacgtaatca ctcgccacga ttgcattatt tgtataaacg cttggcgttg gtacagtatc
     3241 aataataata agatcatagt cactttttaa aggtgctaaa agagtagcaa gcaatctact
     3301 ttcattttca aacgtccatg agcgagttaa ttttggcagt aacatcaaat caaacgtgcc
     3361 agggatcaag tctaaattat cagtcaaatg aataatagaa gaagccaagt ttccattttt
     3421 caagccttca taaaaattga cacgtggcaa ttctacctca aaagtttttg ctaagtcttt
     3481 tgtcaatgtt gcctgtaagt ccttatcgat cattaaaact tttaaattca atttgtctgt
     3541 caagtaagca aacatagtcg ataatttgga ctttccaaca ccacctttaa agtaattatt
     3601 caagataata attgcttcat ttttattgtt taaaatacgt cttaattctt caagtatttt
     3661 tagtttttcc ttctccataa ctacgcccca ttcttttttt gtatagtaat attgtatcat
     3721 taagaatcac aaatcacaag tgatttgtga ttaatcactt gtgatttgtg ataagtgatt
     3781 tgtgattaat atataaaagc cctctttaaa gggcttttat atttattttg agaaagatat
     3841 aaaatcaata tatccctttt ccccaatttt tacaacggca ttgtagggca tatagttttt
     3901 tatatgaaag catttgtttt aatatttgtt ccaaagagcc atcatggctt tgacagagct
     3961 cttcttctat gcaaatgctt ttaatccgat ccacacgaaa attcctatat tcatttctta
     4021 gcctgcaaaa ccgattaagt accagttctg ttcataaaac cccagtgata taggctctat
     4081 cattctgctt tccggttctt gccctcctga tgcaggatat tgaattgata ttaccctttt
     4141 attgcagatt gcagtctgaa ttgctgcaat aacgttatcc gccaatgtat ttttctgtcc
     4201 agatgtgctg tacacgttaa tactgttctc caactcctgt gcatagtttt tttcaccatg
     4261 cttcaaaata gatttgattt tatacatagc tgaatcaaaa tcctttataa atgattcatc
     4321 tccatggcaa tttaaaaatt tccctgctgt aatcaatgca cccacttccg ccgctgtaaa
     4381 cattaccggc ggaagtagga agccctctac aagaaaatat ccttttccgg cctcagatcc
     4441 aattggtatt ccggcttctt caagtgtccg gatatctctg taaactgtcc ttaagcttat
     4501 attaaatcgc tgtgcaattt ctttggcagg aattattttt tttgattgca gctgaataag
     4561 aatagaggtt actctgttaa tcctgttcaa attttattcc tcctctgtga cttccataaa
     4621 aagtatatcc tatgcgttct taagctagct gctgttagtg cttctcattg caccctggtt
     4681 ataaaagcca tactcacaaa cctcttcacc aaaactcttt acatctctaa tatagaactc
     4741 tatctagcga acttttagaa aagatataaa acatcagagt atggacagtt gcggatgtac
     4801 ttcagaaaag attagatgtc taaaaagctt gtagttaaag ctttttagac atctaaatct
     4861 aggtactaaa acaattcatc cagtaaaata taatatttta ttttctccca atcaggcttg
     4921 atccccagta agtcaaaaaa tagctcgaca tactgttctt ccccgatatc ctccctgatc
     4981 gaccggacgc agaaggcaat gtcataccac ttgtccgccc tgccgcttct cccaagatca
     5041 ataaagccac ttactttgcc atctttcaca aagatgttgc tgtctcccag gtcgccgtgg
     5101 gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga
     5161 tctttaaatg gagtgtcttc ttcccagttt tcgcaatcca catcggccag atcgttattc
     5221 agtaagtaat ccaattcggc taagcggctg tctaagctat tcgtataggg acaatccgat
     5281 atgtcgatgg agtgaaagag cctgatgcac tccgcataca gctcgataat cttttcaggg
     5341 ctttgttcat cttcatactc ttccgagcaa aggacgccat cggcctcact catgagcaga
     5401 ttgctccagc catcatgccg ttcaaagtgc aggacctttg gaacaggcag ctttccttcc
     5461 agccatagca tcatgtcctt ttcccgttcc acatcatagg tggtcccttt ataccggctg
     5521 tccgtcattt ttaaatatag gttttcattt tctcccacca gcttatatac cttagcagga
     5581 gacattcctt ccgtatcttt tacgcagcgg tatttttcga tcagtttttt caattccggt
     5641 gatattctca ttttagccat ttattatttc cttcctcttt tctacagtat ttaaagatac
     5701 cccaagaagc taattataac aagacgaact ccaattcact gttccttgca ttctaaaacc
     5761 ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat aacatagtat
     5821 cgacggagcc gattttgaaa ccacaattat gatagaattt acaagctata aggttattgt
     5881 cctgggtttc aagcattagt ccatgcaagt ttttatgctt tgcccattct atagatatat
     5941 tgataagcgc gctgcctatg ccttgccccc tgaaatcctt acatacggcg atatcttcta
     6001 tataagcgta ccggttccaa ttttttcgca gtttaacttt tccgacgcat ttatcgtctt
     6061 ggtagtaaag atatattatc ttatcagtat tgtcaatata ttcaaggcaa tctgcctcct
     6121 catcctcttc atcctcttcg tcttggtagc tttttaaata tggcgcttca tagagtaatt
     6181 ctgtaaaggt ccaattctcg ttttcatacc tcggtataat cttacctatc acctcaaatg
     6241 gttcgctggg tttatcgata tctttcaggt gccctgcttt catttctgta atcactgttc
     6301 ccgcctctct tctatatcag cggcatatgt gctatccagg cagccggttt taccagtgta
     6361 ttttttatac atgtccctgg tatattttgt tatattccta tcatactccg gataggcata
     6421 atgaagcctt tccgccacct caccggatac cgccctgaac aattgatggc atagaaataa
     6481 tgcttcccat atgttttcat aggaatccat ccggtaggtg gacaaaagtt tctcccacaa
     6541 atcctcggat atataccttt caataaactt atagttcttg cctacactta atttaaagcc
     6601 tgtttcgatg ccgaccttcc atgatatcat tctcagcagc tcatggcgaa caatctgatt
     6661 aaaatgatca atagcaaata aaatttcctt acggcacaat cctttaataa cataaggtgt
     6721 tacattccaa aattcattgc agcaatcatc atactccctt gcgcttggct ttcttacatg
     6781 ataatctata tcagtcggaa ctatgtccct tttaattcta caatctttat caattagaac
     6841 ctttattaat ttatcgccct ttaggtaatt atctaactct tccaagggca ataaggtaag
     6901 atcaatttta ttgtaatcat caaatagcat aagataggaa aatccctttt cttcaggtgg
     6961 gaataattcc atatcctccg gcttttgcat cattattata ttcccaaatt gattaagcca
     7021 gtcatcatta gatataaacg gttctatatc acttacaaaa tatgtaatat cataatcctg
     7081 aaattcatct ttaggtatat taatatttgc gcgtgacccc tcaagggtca caattcgaat
     7141 acgttcatcc tgttctgcta aagaaagtac taaatccatc atttcttttt ctgatctcat
     7201 ttacatactc ctcgtttatt ttttctatat tattacatct tcttttttgc cgatacaatc
     7261 agcatcattg ggcgtcgcat ttcatccgcc atccccggaa tatccatcat gttctctggc
     7321 ggctgtggct ccacaatctg atttattata aaactatttg aaagcagtgt atttagatat
     7381 gtggtcagtg ttctatgata ttttgtaacc ttttcttcca aaaacatagc tgtccgtttg
     7441 ccctcataat aataattgtc caccgggaaa tgcagtattt ctcctttttc gttataatac
     7501 cagtcttgtg ttccatgagc agtaaaaaca ggatgttcaa ctgtaaaaac taaattgcca
     7561 ccagccttca gcatcctata tatctttttt attaaattct catagtctgc tacataatga
     7621 aacgcaagcg aacttagtat tacatcaaag ctctcctctg ggaaatccac atcttctatg
     7681 gcacagcatt catattcaat ctgtggaaaa tgggtttttc cttttgctac ttcgagcatt
     7741 ttatgagaaa tatcaacacc tactacagag gaagcaccgt tttccatcgc atatatacag
     7801 tgccatccat agccgcatcc taaatcaagc acacgcttac ccttaaaatc aggtagcatc
     7861 tttttcaaag tctcccattc tcccgcacca gccagtcctt tctgcgagcg actcatttga
     7921 ctgtattttt gaaaaaatat attatcatca tatttgtttt ctttcatctg aactctcctc
     7981 gtttaaaaag ttatttatct ccgatacaat ttcattcact tctttataaa gtttctcggt
     8041 catgtcgtag cattcaaaaa gagaaagacc gagtacttca aaaatatgat taactctttc
     8101 ggcatatttt tcaggtttat attcaaaagt ttcaagcagt tttatagctt tcttttcgtt
     8161 aatgcaataa gcattattac atgcaaatag tacttgattt aagcatgaaa taatacgaaa
     8221 aacatggcct gcaatataat atttatcctc tgcccccgca tttgctttta caaacattaa
     8281 agagaattca gcttcaaata taaaaaagtt catcaagctt ttctttagag cagtagggta
     8341 aatttctgcc tgctttttta attcgcataa gctttcgttc ttagcatata gtattttact
     8401 aatcgctaat tctccacgat acatagcact tatataacca tggggatgcc cggtctgata
     8461 attggcagta acaattcctt gctccgtatc tttgattatt tgttccaccc gttttatatc
     8521 acgtaatatc aagtcaacat gacacccgtt tataactaac catccaccgc cattaaccca
     8581 atcaccccac gctccgggag gtacaacaag gttgtttcta ttctcatcat ccaactctgt
     8641 agcaatttga ttaatcgctg tcaggtcaaa tgagtctgaa ttgtaataga ttccgatatc
     8701 tatatcagaa ttttctgtat gggtgcctct tgcacgtgag ccccctaata cgatgccttc
     8761 tataaaaggc aaagatgata atttctctgt tactatttga ataacattac ctaccatata
     8821 aacatcctcc tttattcgtt agatattttt atttcttctc catctggtaa aataaatgcc
     8881 tgcacaaact tgacacctag cacatcagca atggccttca actcatccaa ggttactgtt
     8941 tctcgttgta attttttatt gaaattctgt ggagtctggc caatacgtct agcaagttcg
     9001 gaaacactta tattcatttg ctcacacagt tctttaatca tttctgccgt agtcatacag
     9061 cacctccaag tttacgctaa gaatattata aaccatttgg ttgataaaca caatagttat
     9121 taaaatatta ttataatcag caagcaaaag aaaacccaca actctactgt agagtcatgg
     9181 gctatttact tctggttttc atatttccat ttcgatgcca gatttgaagg ctatgacgaa
     9241 gtggccttca tagactgtaa cgctctggat tatcttcctt acaagcttat catcatattc
     9301 taaggtgcgg aatttgttct tgcggataaa ttcaatcagc tcattgattc gctcgttctc
     9361 gccactgagg gaggcatctt ctactaaaag gatctgtcgc ttgtctctca gctcatcaat
     9421 ctcatctgct aagtggtcat agtcttgacc cttgttcgcc agcttgatta gttctttttg
     9481 tttttcctct agtgagttgt taatctctga aatttggtat tctgtggttt cgccaatcac
     9541 agcatgaata ttctcttcca gtgtctttat catattgttc ccacctgcaa gtagcttatt
     9601 aatcgcagtc attactgcac cataaagttc atcttctttt acggttcggt tcttacaaac
     9661 ttcaggacct tgctcgattc tagtcacgca tcgccagaca aattcttttc ttccgtggat
     9721 attccaatag acccgtctat aaatatcgcc acaatctcca cagaaggtga tggcacttaa
     9781 agcgtacttg ctactgtaaa ttcttttgtt cttgccttct cctgtgtaga tattgcttct
     9841 ccgatgaatt tcttcttgcg cctgcaaaaa taactcttta ggaataatcg cttcatggct
     9901 attttcaaca taatattggg gaacatgccc ttcatttttc actcgtttct tggtcagaaa
     9961 atctactgtg acagtctttt gcagaagggc atctccgatg tatttttcgt tttgaagtat
    10021 cttcttaact gattctggtc gccatcttgg ttttcccgct gctgttaaaa taccatcctt
    10081 ttcaagggct cgaccaatgc ccactagact ctgaccctca aggtattctc ggtagatgcg
    10141 tttgataatc tcagcttctt cggggacaat agttaaattt ccatcttcat ctttcgtgta
    10201 gcccataaat cgcttatggt tgacctgcac ctttccttgt tggtatcggt actgtagccc
    10261 aagcttaacg ttttgtgaaa ggctctggct ttcctgctgt gccaaagatg ccataatagt
    10321 cagcaaaacc tcacctttgg catccgtggt gttgatgttc tctttctcaa aataaactga
    10381 tatgttctta tccttgagct gtctaatgta ttgaaggcag tctagggtgt tacgcgcaaa
    10441 tcggctgatg gattttgtaa taaccatgtc gatgttaccg tccatacact ctgcaatcat
    10501 gcgattaaat tcgtcacgct ttttagtgtt cgtaccagag ataccatcat ctgcaaagat
    10561 gccagcaaac tcccattcat tatttttctt tataaactct gtatagtgtg agacctgaac
    10621 ctcatagcta gaattttgtt cttctgtttc tgtagaaaca cggcaatagg cagcaacacg
    10681 aagtttcttt atcttttctt ttgcggctgt acttcctact cttttacgag caggaataac
    10741 cattatattt ttctctgtca ctttattcct cgctttctat cagactgtat aagtattctg
    10801 ctcgttcaaa aggatccact ggcatcttat tatctgcctt tttcattttg aatcgttctt
    10861 tgggaggggg agaggtgaaa gcggcaagct ctaccactcg tcctaaatcc tttgcacgtt
    10921 tatctctaac ttcttcagct ttatcaaata tctctttatc aataattgct ggatacgtat
    10981 catttccaag gtagttgacg tttttcaaaa tgcgccccat cacagaatgt gtcttttcaa
    11041 tacctgcctg ttcgccagcc actgtaaggg atagtcctga tatgtatttc tcaaagaata
    11101 cctttacttg acctgctgcc ttttcatcga cagtaaccac tccatcttga attttgtatc
    11161 catatggaat atatgccatt tatctcacca ccttttcttt cagggaaaga ccgcatttca
    11221 aattgaatgt cagctcatcc ctggaattta caatgatgtt cactacaaat tcttcaaata
    11281 gttcttccgt gtagtcacca ttaaaattat ctgctgacac ataattaatg aggtccttta
    11341 tctcgtttgc tcgcaaaacc ccactcgcag agttcgttac caggtttgtt ttttcggtag
    11401 taagattttt aatctcacta tctaaaacat ttcgttcctg attaaaaaga gcaggttcta
    11461 ggaaaccttt ggacatcagt gcaataaggg tattgcgttc ttccaatagt tgctccatta
    11521 gcttatcaat agcgtccatt ctttcacagt cgctttcttc atcgatttgg ctaactgact
    11581 tgaaaagtgg ttctaggatt agcttatgac tgaaagcaag cttattcatc atggttgtca
    11641 atgtggcttt gatttcccca tctcgcaaaa acaacatgga gcaactttct ttatcttcga
    11701 tactatctca acatgtgtcg tggataaggt aagtatgtaa aatggtatgt ggaacatagt
    11761 gatttgctgt aaatgatttg ctaactactt tagttcttta tacaaagtat aaatcggata
    11821 ttttattatt ttttctttta tatcttctga gattaggtca aagtatttgt ccgctaaatc
    11881 agaaaggggt tcttttttca tcacctaatg ggtgatgaag cagaatactt taaagactat
    11941 taaatcaatg ttaggaaaga ctattagtaa aactatgaat tattcaggaa aaaaggttct
    12001 tttttaaagt tttctattgc ttcctggcgt tgataaatga gatataaagt cggtgtttgt
    12061 acacgtccta atgaaaatgt accttgtacg cctttttgtt gtaaattcaa ggtatataaa
    12121 gggcttgcgt tcatgccgat caaccaatcg gcaatttggc gtgtttgcgc ttcttgataa
    12181 aagggatagt aattcattcc tggttgcaaa ttttgaaaac cgcttcggat cacatctttt
    12241 tctaagctat tgatccataa tcttttatac gttttatctt tagaaaaggc atttgcttta
    12301 tggataatcg accaggcgat attttcacct tctctgtcgc tatctgttgc gacaataatt
    12361 gtatttgcct ttttgagaag ttcagcaaca attttaaact gctttccctt atcttttgca
    12421 acttcaaaat cgtatcgatc aggaaaaatc ggcaaagatt caagtttcca attttgccac
    12481 ttttcgtcat aatgacctgg ttctgctaat tccactaaat gcccaaaacc aaaggtgata
    12541 aacgtttcat ctgtaaatag tgggtctttg atctcaaaat aaccgtcttt tttggtgctt
    12601 tgttttaaag cacttgcgta ggctaatgcc tggcttggtt tttcagctaa aataaccgta
    12661 ctcattaact atccctcttt tcattgtttt ttctttgatc gactgtcacg ttatatcttg
    12721 ctcgatacct tctaaacgtt cggcgattga ttccagtttg ttcttcaact tctttatcgg
    12781 ataaaccatt caaaaacaaa tcgaaagcat gctttaaacg tggatcattt tctttaaatt
    12841 tatgctgacg acctttgaat ttgccttttt tcttagcaat ttcgattcct tgtgcctgac
    12901 gttccttaat tttttttcgt tctgattctg cttgatactt gtacaattca atgacaaggc
    12961 tattaatcaa acgccttaaa ttttcatctt caataccatt cattgagggt aaatttaaga
    13021 cttccagggt tgccccctta atttgaattt gattcatcaa ttctgttaat tctttattat
    13081 ttcgtcctaa tcgatctaat tcagtaacaa taacaatatc cccttcacga atatagttaa
    13141 gcatagcttg taattgtggg cgttcgaccg attgaccgct taatttgtct gaaaagacct
    13201 tagaaacgcc ctgtaacgct tgtaattgcc gatctaagtt ctgttctttg ctactgacac
    13261 gtgcataacc aattttagcc attttcaacc aacctctaaa attctctcgg ttgcaataac
    13321 caatcagcaa tatctacttt ttcaatttca aattgcttat cagaaattgt cttttcgtaa
    13381 gcgataaaat cttgcgcata ttgttgctca ttaaaaatag ccaccacttc gtcattttct
    13441 aaaactcgat aaataaattt tttcatttta ctcctcctat tatgcccaac ttaaatgacc
    13501 tattcaccaa gtcaattata ctgctaaaat catattagga caaataggta tactctaatg
    13561 acctataaaa gatagaaaat taaaaaatca agtgttcgct tcgctctcac tgcccctcga
    13621 cgttttagta gcctttccct cacttcgttc agtccaagcc aactaaaagt tttcgggcta
    13681 ctctctcctt ctccccctaa taattaatta aaatcttact ctgtatattt ctgctaatca
    13741 ttcgctaaac agcaaagaaa aaacaaacac gtatcataga tataaatgta atggcatagt
    13801 gcgggtttta ttttcagcct gtatcatagc taaacaaatc gagttgtgtg tccgttttag
    13861 ggcgttctgc tagcttgttt aaagtctctt gaatgaatgt atgctctaag tcaaaagaat
    13921 ttgtcagcgc ctttatatag ctttcttttt cttctttttt tactttaatg atcgatagca
    13981 acaatgattt aacactagca agttgaatgc caccatttct tcctggttta atcttaaaga
    14041 aaatttcctg attcgccttc agtaccttca gcaatttatc taatgtccgt tcaggaatgc
    14101 ctagcacttc tctaatctct tttttggtcg tcactaaata aggcttgtat acatcgcttt
    14161 tttcgctaat ataagccatt aaatcttctt tccattctga caaatgaaca cgttgacgtt
    14221 cgcttctttt tttcttgaat ttaaaccacc cttgacggac aaataaatct ttactggtta
    14281 aatcacttga tacccaagct ttgcaaagaa tggtaatgta ttccctatta gccccttgat
    14341 agttttctga ataggcactt ctaacaattt tgattacttc tttttcttct aagggttgat
    14401 ctaatcgatt attaaactca aacatattat attcgcacgt ttcgattgaa tagcctgaac
    14461 taaagtaggc taaagagagg gtaaacatga cgttattacg ccctattaaa cccttttctc
    14521 ctgaaaattt cgtttcgtgc aataagagat taaaccaggg ttcatctact tgttttttgc
    14581 cttctgtacc gcttaaaacc gttagacttg aacgagtaaa gcccttatta tctgtttgtt
    14641 tgaaagacca atcttgccat tctttgaaag aataacggta attaggatca aaaaattcta
    14701 cattgtccgt tcttggtatg cgagcaatac caaaatgatt acacgttaga tcaactggca
    14761 aagactttcc aaaatattct cggatatttt gcgaaattat tttggctgct ttgacagatt
    14821 taaattctga ttttgaagtc acatagactg gcgtttctaa aacaaaatat gcttgataac
    14881 ctttatcaga tttgataatc atagtaggca taaaacctaa atcaatagcg gttgttaaaa
    14941 tatcgcttgc tgaaatagtt tcttttgccg tgtgaatatc aaaatcaata aagaaggtat
    15001 tgatttgtct taaattgttt tcagaatgtc ctttcgtgta tgaacggttt tcgtctgcat
    15061 acgttccata acgataaacg ttgggtgtcc aatgtgtaaa tgtatcttga ttttcttgaa
    15121 tcgcttcctc ggaagtcaga acaacaccac gaccgccaat catgcttgat tttgagcgat
    15181 acgcaaaaat agcccctttg cttttacctg gcttggtagt gattgagcga attttactat
    15241 ttttaaattt gtactttaac aagccgtcat gaagcacagt ttctacaaca aaagggatat
    15301 tcattcagct gttctccttt cctataaatc ctataaaata ggttgtttaa ttaacttggt
    15361 ttgctttttc attcaactgt ttcaatattg catgttttga aaaagatttt tttcctttat
    15421 aagtcaattt ttttccacta atcgaataaa ttattttgtt attttctatt aacttatata
    15481 tataatcttc cccctccgaa gaaaaatact tatctgattt tgtttctaag tagatatttc
    15541 tcttttctaa ctctttctta aacgtttcta gtgtatagat atttgctaat tttcttatct
    15601 ccaataaact attttttata taagttttac attcatcatg attcatacaa actccacctt
    15661 ctataaatga atacaaaaaa agcaatcaaa cgatttccga ttgattgctt aacaattctt
    15721 aaattcagta gcttagatac ttgaaaactc tctgatttcc ctatataatg atagtacggt
    15781 tatataccgt cttcaaacaa agttaattaa ataacttctt acgagggaag agttcatctg
    15841 actaactgat aagcgttggt ttggcaatct tatcgggcta tgcatttata aaatgtcgtc
    15901 aaacatttta taaatgtgtc atggctcttt tttcgtttct attcagttcg ttgtttcgtt
    15961 atatctagta taccgctttt aaaaaaaata agcaacgatt tcgtgcatta ttcacacgaa
    16021 gtcattgctt ttttcttctt ccatttctaa atccaatgtt acttgttctg attctgtttc
    16081 tggttctggt tctgttggct catttgggat taaatccact actagcgttg agttagttaa
    16141 ctttgcaatt tgttctagtg tttttatggt tggatctgat tttcctgatt ctattcgtga
    16201 ataatttgat ctactcattt ctaattcttg ggagaatttt tcttttgatt tggtacctct
    16261 catctttttt aagctttctc taaatgctag ttccaagttt ctcccccctc tttttgtgta
    16321 ataaaattca cgctttattt aaattatagc ttttaatatc ttaataaaca agtgattaat
    16381 cacttgtgat ttgtgattat tccaaaagta ggtaatttca ccaacctttt tcaaaagttt
    16441 ttatcgacga ttattggact ggatcgtgtt tcctaaagcg attctaagcg catgtttaat
    16501 tttaaagtgg gtaattctct ttaaaacgtc ctacaaagat tcaaaaaaag catggctgct
    16561 agtgggggta ccgtcaggaa aatgcggatt tacaacgtta aggaaattta ttaaagagac
    16621 caattcctca gcactaaaat tgataattgg gctttctttg ttactatagt gttaggtaag
    16681 ctttttactt tatcaccggt tttcccctca cttcacacct acgggcgagt ttccccacat
    16741 cccgttttcc atctaatcca attcattcaa tatctttatg tgtatgaaag atcagattgc
    16801 ttgatttaga catcttacac gtaatttatt cttaaatggg tacggtaaac gagcaataat
    16861 acgagcaaaa gtatacctac aaaaataata actctgtcta attttttttg attcatatgc
    16921 ttattgctta aaccatataa tttcagtcca agaaagcctc caacagtatt tgtaattaca
    16981 tctgttatgt ctgtcgctcc aatagcgaag ataaattgaa ttatttcaaa agtaagactt
    17041 aaaaccagta caaaagcaaa cttaggtaaa aatccgattt ctttaaaatt gacattcaaa
    17101 agcaagccaa atggaataaa gattataaca ttatctatca tctctctgaa attcccagta
    17161 gcagtaaatg gagtcaagtt aagacttctt tgatgataat taaatactga taaaatattg
    17221 tattgtaatt tgaataacac taaccagatt agtgtcacta aatataaagc tagcaatcct
    17281 ctagataata tttttcccaa tataatttcc tctaattctt ttgtttaagt tgtatcatta
    17341 gtatagcaat gtcctagtta acgtgggttt taaattaaaa agctaacgtt accccaaaag
    17401 tgctagtaca ataatgctgt tcattaattt cgttggtttc attccgccat ccttacctcc
    17461 ttgaattagt atgtgttgat ccgggaaaca ctgtcacaat tacaccgtct atattgtttc
    17521 ctgatatctc ataacgaaga ttagtcggca catgaatggt ggtattttta gtaacaggat
    17581 aataaaagat ctcatatttt tccccattta cactaacaga aatggttttt tcttctttta
    17641 ggtaatccat atattcctcg agaacgaaat tcttttcttt cataatcgca ctatgtggta
    17701 aaccaacata gcgaatatgc catggttcat attgaattcc tgttaactct gttttgtcct
    17761 ctggataacg taaaatgaac ccgtatttcc aagcattttc ttctatccac tttccttcag
    17821 gggctcgttc cattttcgtc aagcttgatc ctacatctag tgataaacct gaattatgct
    17881 cactataacc tgctggtaag gcatactcag cccccatttc ttggtaaagc acactttgct
    17941 catcaaagtc tcgatagcca ctattaataa taaaatgact aacgccaccc tttacagcat
    18001 cattgaccat ctctgaaaat ttttgtgcta tttcttttga catataaata ttactatcaa
    18061 gcaacccgta tccatttatt aattcgtcat gtttagataa attcacgata tctgacttca
    18121 cactttcttg gcgaacagga tatttactat tgattaatag cagatttcct tgataaacct
    18181 gttcttctgt aattgttttc tcttgggtat tttcagaagt cccactattt tctaaatgtt
    18241 ctttgggatt ttgatcataa ttttgaaatt cgactttttc ctgagaaaac agtgcttcat
    18301 taacgtagtc ataacctaag tatattaaga ataacaataa caataaaaaa aacaacttct
    18361 tcattttcag tctcctcctt aactatatag ttggaagtat gaaatatttc tagaatcttc
    18421 cccttaaacg gcacaatctc gtttgagatt gtgccgtttt gccccgtaaa ttacagtcaa
    18481 ttatttcgcc ctactggatt tactattatc accaatgtag atgaattttc ataagtgata
    18541 cccatgctga atttaacgag gctgaactgc ctgttgacaa tgg
//
LOCUS       DARBDV010000062        18188 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000062 DARBDV010000000
VERSION     DARBDV010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.062"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(28..726)
                     /locus_tag="SCM56_001941"
     CDS             complement(28..726)
                     /locus_tag="SCM56_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEG1110703.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(719..2071)
                     /locus_tag="SCM56_001942"
     CDS             complement(719..2071)
                     /locus_tag="SCM56_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEG1110704.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2294..3694)
                     /locus_tag="SCM56_001943"
     CDS             complement(2294..3694)
                     /locus_tag="SCM56_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEG1110705.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3855..4592
                     /locus_tag="SCM56_001944"
     CDS             3855..4592
                     /locus_tag="SCM56_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEG1110706.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4717..5196
                     /locus_tag="SCM56_001945"
     CDS             4717..5196
                     /locus_tag="SCM56_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HEG1110707.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5193..5960
                     /locus_tag="SCM56_001946"
     CDS             5193..5960
                     /locus_tag="SCM56_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110708.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6190..6759
                     /gene="lepB"
                     /locus_tag="SCM56_001947"
     CDS             6190..6759
                     /gene="lepB"
                     /locus_tag="SCM56_001947"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEG1110709.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6863..7465)
                     /locus_tag="SCM56_001948"
     CDS             complement(6863..7465)
                     /locus_tag="SCM56_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEG1110710.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7571..8040
                     /locus_tag="SCM56_001949"
                     /pseudo
     CDS             7571..8040
                     /locus_tag="SCM56_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8146..8730
                     /locus_tag="SCM56_001950"
     CDS             8146..8730
                     /locus_tag="SCM56_001950"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEG1110711.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8733..9806
                     /gene="prfA"
                     /locus_tag="SCM56_001951"
     CDS             8733..9806
                     /gene="prfA"
                     /locus_tag="SCM56_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEG1110712.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9799..10638
                     /gene="prmC"
                     /locus_tag="SCM56_001952"
     CDS             9799..10638
                     /gene="prmC"
                     /locus_tag="SCM56_001952"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEG1110713.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10631..10810
                     /locus_tag="SCM56_001953"
     CDS             10631..10810
                     /locus_tag="SCM56_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110714.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10832..11857
                     /locus_tag="SCM56_001954"
     CDS             10832..11857
                     /locus_tag="SCM56_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEG1110715.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12032..13276
                     /locus_tag="SCM56_001955"
     CDS             12032..13276
                     /locus_tag="SCM56_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEG1110716.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13429..14376
                     /locus_tag="SCM56_001956"
     CDS             13429..14376
                     /locus_tag="SCM56_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG1110717.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14510..15139
                     /gene="upp"
                     /locus_tag="SCM56_001957"
     CDS             14510..15139
                     /gene="upp"
                     /locus_tag="SCM56_001957"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEG1110718.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15278..15520)
                     /locus_tag="SCM56_001958"
     CDS             complement(15278..15520)
                     /locus_tag="SCM56_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110719.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15641..15713
                     /locus_tag="SCM56_001959"
     tRNA            15641..15713
                     /locus_tag="SCM56_001959"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15674..15676,aa:Thr,seq:cgt)
     gene            complement(15832..16470)
                     /locus_tag="SCM56_001960"
     CDS             complement(15832..16470)
                     /locus_tag="SCM56_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEG1110720.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16593..17225)
                     /locus_tag="SCM56_001961"
     CDS             complement(16593..17225)
                     /locus_tag="SCM56_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEG1110721.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17576..17875
                     /locus_tag="SCM56_001962"
     CDS             17576..17875
                     /locus_tag="SCM56_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEG1110722.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT         6250 a         3165 c         3579 g         5194 t
ORIGIN      
        1 tttgtcacat tccccttttt cgaataacta ggctgtttgt ccttcaagat agggcagatc
       61 caattcttta ccgtaacgtt gttctaaagc taggcggatc aaacgagctg ccaggttctc
      121 atttcttgca aggataggtc catggaaata agaaccgaag acatttcggt aaatgacacc
      181 ttcagattgg tcttcaccat tatttccttg accttgtacc actttcccca aaggtcgttc
      241 accttcacct aagaaagtcc gaccattgtg gttttcaaat ccataataag tttcattgaa
      301 ttcttcgtta tggataacga tatcaccgat aaaccggtta ttttcctgac ttaacgtgta
      361 gtgatccagt gcactgattc cttggatctt ttctccatta gctcccatgt agtagtgacc
      421 tagcaattgg tagccaccac aaatggctaa catgacgcca tcattttcga tatagttgat
      481 caaagaatct ttctttttct ggatgtcttt tgaaatgatt aattgctcaa agtcttgacc
      541 accgccaaag aaaaccaagt catatttatc agcatcaaaa ggttcatgta tactaacgat
      601 ctccgtatgg caagaaaccc ccattttttc tgctatatat ttcaacatca aaaggtttcc
      661 gttatcacca tatgtgttca tcaaatttcc ataaaggtgg gcgatattga gttcataatt
      721 agccacggtt cattcctcct ttgataaatc cttgagcagc tagttcttta cgaagctgca
      781 atacagcagt gtaagtagct aaaatataga catgctcagt tggcaattct ttgatttttt
      841 cgattacttc tggtaattct tttgtttctg tcaatttatt ttctggaatc ccggcaactt
      901 ttagacgtaa agtcatatct gtatgacggt ctcctccaga aataacagct gggatatcca
      961 tatcggcaaa gttttcaaaa ttaccatccc aaatccagct gacatcgatt ccgtctgcgt
     1021 aattggcatt taataaagcc actaaagaaa atggataagg agctagacca atcatatcga
     1081 ttacttgatt gatacctaca ggatttttga caagtactaa tgtacatttt ttcccattga
     1141 tattgatgac ttcttgtcgg ccgaatactt tctcgtcata tcctaaacct tgtttgattt
     1201 tatcaggatc gacattgaag tactctgcca ctgcagtagc tgctaaagca ttgtagacat
     1261 tatacatacc gccaacttcg attccgtatt catgaccatc gatcacgaaa tctgctgaca
     1321 cattgtccat ccgtaccata tcagttaatc gataatccaa ttcaggacgt ttgaagtcac
     1381 aatttggaca gtaatatttc cctaagtttg cataagtgat cattttataa tgcaagatgt
     1441 gatgacaatt tggacataag acaccgtctg tattgtaatg agccatttgt tcacgatctt
     1501 cttcatggtc aaagccataa tattttctag gattgactgt ttcgatagaa ttgaagattg
     1561 gtgaatcacc gttactgata atcgttgcat ttggtgactt tgcggcaccg tctacaatca
     1621 ttttataggt cgtatagatt tctccatacc gatccatttg atcacggaaa atatttgtaa
     1681 ataagaacag ttctggtttc atatattctg tgactttgct caaacttgct tcatctattt
     1741 ctaggacagc aaactttttc tgtccttttt ttggttttgc ctgcaagaaa gtcgatacaa
     1801 ttccctgtac catattcgca ccagtagggt tggttagaac ttcatcgaat tgctggcgca
     1861 aaatattgac tgttaaagcg gtagtcaatg ttttaccgtt tgttccggtg acaacgacga
     1921 cttggtagtc ttttgccaaa ctatctagta tatgtggatc gactttcagc gctagtttac
     1981 ctggtaaact tgatccgcct ttaaaaaaag tttttaatgc ccattgcgac gtttttccaa
     2041 ctgcaatcgc aaaatgactg cgtattccca ttatgtaacc tccaaaaacg atttgctgac
     2101 gaataaattc aactaatcat accatatgtt gaaaaaaagg ttaaattgct tttcttattc
     2161 ttaagaaaag gttacggctg aaagtgcatg aaatcggaca aaatcgcatg atttaacaaa
     2221 aaaacagctt gaaacaaaat gattgtttca agctgctgtg attttattat tccctcttac
     2281 ctatattccc attttaaccg ataataattt tctcgcttgg atattcatag cctagatctc
     2341 gtgtttcttt tggcacgaat aacatcaatg tatatagcat tccgatccgc ccaataaaca
     2401 tcagtgtagc aatcgtgatt ttgcccacaa cagacaagtc tccggtaatc cctaaagata
     2461 aaccagttgt tccaaacgca gaagttactt cgatgatgat cgagatcagc gtttgttctt
     2521 ctgtagcaga taaaaaaacg atacaaaaga aacacatacc aagagaaagc ataaagacaa
     2581 cgactgattt tcgcacatca tcttgatcga ttctccgtcc aaaaatattg atgttatctt
     2641 cacttttgag gaaggaatat agatacaaac cgatgattgc tacagtagtt gttcgaatcc
     2701 cgccgcctac agaactagga ctgcatccga taaacatcag taaagaaaaa atgatcagtg
     2761 ttgtgatttg aaaatcaccc aaatcgtgaa tctgcagacc agcattacga gtcgtaatcg
     2821 aataaaacat cgaattgatc catttgacac tcatattgga atcttggaat aaatggtcct
     2881 tttccaatag ataaatcaag acagttcccg aaacaaacaa gataacaaaa gctaacacag
     2941 ctaatttggt aaacaaggaa aaacgaaacg gaagcttcgc attggaacgt ttgtataata
     3001 gccattcccg gcactccatc aatacaggaa aaccaattcc tccgacaaaa atcaaaaaca
     3061 tgatcaggat caagaaaaaa tagtcatgtg caaagggctt gatggaatcc ccagtgacgt
     3121 caaacccaga gttcgtgaca gccgatatcg cctgataaaa accgtaaaaa acggcatcac
     3181 gccaacgatc aaaatagcct ctgtaataaa aataaataga aaagaatgtc ccaaaaagta
     3241 attgaaacca gattaaaata gcaaaagtga tccgaatcag tcggacgata ccagacaaac
     3301 gcggttgatt catatccgtc atgatcagct gtcgctgttt cagtgtgatc cgtcgtttag
     3361 aaaaaatgac aaaagcagta gaaatcatca tgatccccaa accaccaact tgaaataata
     3421 tttccagcag tataataccg ttatcattga acacagaatt gatatcaaaa gtcgatagtc
     3481 cagtcacact cactgtactg attgccatga acagcatgtc cacaaaagaa acatggctat
     3541 ctggttcgcg aaaaatcggc atataaaata gaacgagtga taagaccgtc atcaatatat
     3601 aataaaaaac gatgatttgg atcgatgaga aatgattggc agcaaacttc tgtcctcgcc
     3661 gctgcagcct tcgaaagaat aaatcaacat gcacagtact atcctcctga caatagtcgt
     3721 ttctaccagt atacaagggg aattaggaat actcaaagaa attatcaaac agataacaat
     3781 acagctttcg attaaaatag aaatggctta taataaggaa aatcctagaa aaaagaagga
     3841 ggaggaataa caaattgatt aatgctcaac aaatcgtaga aaaaatgaaa aatcaaaaaa
     3901 taaattatga tacagttctg aaaaaaatga tcatgcaatg ggagaaagaa gaagtaaagc
     3961 ctaagatatt aatacataca tgctgtgctc cttgcagtac gcacacattg gaattcatgt
     4021 ctcaatatgc agagatcacg ttattttttt caaattcgaa tattcatcct aaaagtgagt
     4081 atttgcgaag attacatgaa caaaaacgat ttgtagacca atttaatgag aaaactgacc
     4141 atcatgttgc gctaatcgtt gatgaatacc gtccaaatga attcaccaaa atggttttaa
     4201 caaaccatct tgaaaacgaa aaagaaggtg gtgctcgctg cagtgcatgc ttcaatatgc
     4261 gattagatct tgttgcgcaa aaagcgcaag aactaggata cgattacttt ggaagtgccc
     4321 tgaccatctc gccaaagaaa aatgcagctt tgatcaatca gatcggaatg gatatccaaa
     4381 agatttacgg gacacaatac ttgccaagtg atttcaagaa aaacaaaggc tatgaacgct
     4441 ctttgcaaat gtgcaaagag tatgatattt atcgccaatg ttattgtggt tgcgtgttcg
     4501 ctgccaagca gcaaggatgc gatctgaaag aaatcaatcg ggaagcaaaa gcttacttaa
     4561 aaacatcaac cgtagttttt gaggaaaatt aaagaaatct ttcggaaaaa cgtaagaaaa
     4621 aaataaagaa tttatggtag aaataaggta gactgtataa tttgtccgta gtaaagtaag
     4681 gaaacaagaa taaatttcaa tgaaggaaga gataagatgc gaaaagtagc acctattttg
     4741 aagccaatcg ctgtattaac aatctcgacg attgtcgcga ctactacagg tattgtgaat
     4801 agttccagct accgtgcacc aagttcaagt tctagtactg tagaagaaac aacaccaact
     4861 acttcagtct cgacatctga accaacaaca acggaaagtt tgaatgaaac cactacgaca
     4921 agtacatggg acgatacaac aactagttcg tcgtatgatg agacatatac aactgacagc
     4981 tcaagcgaaa caagtaatac ggtaccagat ggcactccat cggattcaac caatccaagt
     5041 acaagtccgg atacaagtgc tagctccgaa acgataccaa gcagtacgac tgtagatgat
     5101 aacacgcaaa atgatgatgc agcacgtaca gcacagcaaa accaagattc aggaaatacc
     5161 caaacgtata accagcaagc aggtgattct caatgacatt tttactagac cttatcaacg
     5221 gggtgcataa attccttggc tatttagaca tcagtcctaa atatcttaac cgtggatata
     5281 cgattttaag tgtgattccc acgctttatt tattgagaat tgtttatgga ctttggcaaa
     5341 accaaaacta tcttcaattt ttcttatatg gacttgcatt tctagtgctt gtttatttta
     5401 ctgttttaaa tgtattttat tactttttag ataaaaacac gaaagccgat gtcactcagc
     5461 tttttgtgaa gtatttacct gatgaagcgt tcaacatcca gacagaagaa tcgatagata
     5521 atggaaattc gatcgataaa gtgaatacac aagaggtcat ggtttcttat gacgaagatt
     5581 atcaattgaa attagccgag aatatgcggt atttgattgg aaatggtgag atcaaaacaa
     5641 atgaactcgg tcgcatggat ggatttctgg tcgatagaaa tacattgtat ccttattact
     5701 atgtcaagaa aacaggagag aaagagtatg ttctaaatat tggacgaagc tactcggaca
     5761 tggaaaaagt agggaccatt cgacttgact caacagaaga cacattggaa cctgtcggat
     5821 tgttcatcgt cggcggtgat tttgtcaaag acgggttacg ctatcatgag ccttatcgtc
     5881 tgaaattgat tgcaaaaaag gcacagtcgc aggaagaacc agtggtttat agtcgtagcc
     5941 aaagaagaag acattcataa tgtgatgaaa cgccgtgtat cagtggttgc gataaaaacg
     6001 tttattcgga ataaggggga tgtgacacgt cttttgtcac gttccttttt tatataacga
     6061 gaaagataag acgaacacat cacttattct ttcctttcat ttataaacat tgtagaataa
     6121 agaaagtaca acgaagtatt gtgaaaaaac gtaataaata acgaggaaaa tacaagtagg
     6181 aggtaagctg tgaaagaatt tttgaaaaat tgggggattt tgatccttgt attagcggct
     6241 attttattag ctagagtgta cgtgtttaca ccagttactg taaacgggca ttcgatggac
     6301 cctacattaa gtgatggaca acgattgatt tcatctaaga tctcaaacta tgaacgaatg
     6361 gatattatta ctacaaagga accgggggat gaagaacgga tgattgttaa acggatcatc
     6421 ggtatgcctg gagatacagt caagatggaa aatgaccagt tgaccattaa tggcaaaaag
     6481 tatgatgaac cttatctgga tgagttcaaa aaagaatttt cagaagataa gttacaagga
     6541 gaatatgctt atagctcagg attccaagca caagcagaaa gttcatccac ctttacgagt
     6601 gactttgaat atactgtgcc aaaaggaaag tatcttgttc taggcgataa ccgcttgatt
     6661 tcaaaagaca gccgaatgtt tgggttagtc gacaaagata tgatccaagg aaaagttgtt
     6721 ttccgttatt ggcctctttc agaaattaaa atcatgtgat gaaaaaataa aaagaagctg
     6781 tgacatgttt tttgtcacag cttctttttc cgaaaattcc ttcttatttc ttggagttgc
     6841 tcactttttt tgcaatctca ttttaagctt tttggctgat ggataagaaa agcgaagcgt
     6901 ttttgttcat aaatagcttt tccccacgat ggaaggtatg actagtagca gagacaaagc
     6961 ccgttttttc caatcgatta ttcaattctt cttggctaaa gccattatgg actttcggat
     7021 gactaatttg ttcattcttg tcaaagtcta cgatgatcaa ttgaccgcca ggagctaaaa
     7081 tttcataaag ttttgtaaga atattctccg tatcaggaat gtgtaaaagt acaagtgata
     7141 gaaggatgag attagcttgg acagcaggaa attcgacaga agcatcagcg tggatcgttc
     7201 ggacattttt cagatctgct gcttggatct tttcttctgc cattttcagc atagtttctg
     7261 aagcatcggc aatgatcagt tctttgaatc tttccgcaag cggtaaacta acaagcccag
     7321 ttccgcctcc gtaatccagc aggacttttt ggtggctgtc gcgcggtaga taagtagtta
     7381 gttcttcgcg aataatcttg gctaatgctt gacggtcagg ggaatcataa tggttagcga
     7441 tggaatcaaa aatatttttt gtcatcaaat agctccttac gtaaaacttt gaaattttaa
     7501 cttattatag cacgtgaaaa gaaagaaagc atgctatact caattcaaaa agggagaatc
     7561 tagaaagaat atgccgatat cgtttgtaaa agatagagag gaaaaaggaa agtgtgtccg
     7621 agaaatatta ctagacttgc ctgagtggtt tgggcttcct gaatcaacgg aaaaatatat
     7681 cgaggaatca agtaaactac ctttatggtg tgaaaaaaga aaagaagaat atttaggctt
     7741 tatcactctt tcacagacaa gtgaagatac tgccgaaatc tattctattg tatgggaata
     7801 aaaaaagact atcatcacca aggattagga acaaaacttt ttaaagaagc agaaggttac
     7861 gcagcaaaac attacaaata tttgcaagtg aagactgtag acgaggggca ctactctata
     7921 tatgatcaaa ctatttgttt ctacgaatca ttaggatttt caaggttgga agtttttcca
     7981 aatttatggg atgaatggaa cccatgttta gttcttgtca aaaaattgga acaaaagtga
     8041 gcaatagatt taatactaat ctgtgcttga tgaaaattct cactgtaaaa aatgctgaaa
     8101 atcaagtata ataccatagg aacaaactgg aaggtgaaaa agagcatggc acaactattt
     8161 tttaagtatg gcgcaatgaa tagcggcaag acgatcgaga tcctaaaagt cgcacataat
     8221 tatgaagaac aaaataagcc agttgtctta atgacaagcg gtattgatac aagagatggt
     8281 ttaggcgttg tttcaagccg aatcggactg aaacgtaatg cgatgccgat atttgaagaa
     8341 acagacgtat ttgacgtaat tcagaaaatg gaaagcaaac cctattgtgt gctgatcgat
     8401 gaagcgcaat tcttaaaaaa gaaacatgtc attgctttta cgagaattgt ggacgaattg
     8461 gatattccgg ttatggcatt tggtttgaaa aatgattttc gaaacgagtt atttgaagga
     8521 tcaaaatatt tattgcttta tgcagacaaa atcgaagaaa tgaaaacaat ctgttggttc
     8581 tgccacaaaa aagcaatcat gaatttacat tatattgatg gaaaccctgt ttatgaaggc
     8641 gatcaagtgc aaattggcgg aaatgaagct tattaccccg tttgtcgaaa acattacttt
     8701 cacccatcaa tgattacaga aggagaataa gcatgtacga tcaattacaa gcaattgaag
     8761 accgttatga agagcttgga gaacttttaa gtgacccaga agtgatttct gatacaaagc
     8821 gttttatgca gctttcaaaa gaagaagcaa acacgcgcga aacagtcgaa gtttatcgtc
     8881 gttataaaga agtagtacaa ggaatcaccg atacagaaga actgcttggt gaaaaactgg
     8941 atgatgaaat ggctgaacta gcaaaagaag aattatctga attaaaaaaa gaaaaagaag
     9001 tcttagaaga aaaaatcaag atcttgcttt taccaaaaga tccaaatgac gataaaaata
     9061 tcatcatgga aatccgtgga gcagcaggcg gagacgaagc agcattattc gctggcgatc
     9121 ttttcaatat gtaccaaaag tatgctgaag cacaaggctg gaaaacagaa gtgatggaag
     9181 ctaacgttac cggaatcggt ggatataaag aagtgatcat gatgatcacc ggagacaacg
     9241 tatattcgaa attaaaatat gaaagtggtg cgcaccgcgt ccagcgtgtt ccttcaactg
     9301 aatcgcaagg gcgtatccat acttccaccg caacagttgt cgtgatgcca gaagctgaag
     9361 aagtagagat cgatattgaa gacaaagata tccgtacaga tatctatcat gcttcaggtg
     9421 ctggcggaca gcacgtcaat aaaacagcat ctgctgtacg tttgactcac ttaccaacag
     9481 ggatcgttgt tgctatgcaa gatgaacgtt ctcaaatcaa gaaccgtgaa aaagcaatga
     9541 aaatcttgcg tgctcgtgtg tacgataaga tccagcaaga agcacaaagc gagtatgatg
     9601 ccaatcgaaa atcagcagtc ggaactgggg atcgttcaga acggatccgg acgtataatt
     9661 tcccgcaaaa tcgtgtgact gatcatcgta tcggattaac tatccaaaaa ttagatcaga
     9721 ttttagcagg gaaattagat gagatcgttg atgcattgat catgtatgat caaacatcaa
     9781 aactggaagc tatgcaaaat gggtaaaatg acctatcgag aagtcctgtc acgggcttct
     9841 tcttttttag aggatcatgg gaaagaagca tatagtatcc aatttttatt tttggaacga
     9901 aaacaatgga aaaaacttga ttggctcctt catatgaacg aagagatttc agaagaagaa
     9961 cagcggttga tcgaaaccga tctgcgactt ctgcttgcag atcatccacc tcagtatttg
    10021 ctaggatatg cagattttta tgatcatcgg ttaaaggtaa ctgaagcaac actgatccct
    10081 cgaccagaaa cagaggagtt agtcgaatgg tgtttagacg agacgcctgg tgtaccgtta
    10141 gaagtgatag atattgggac agggactgga gcaattgcta ttagtttgaa agcagcaaga
    10201 aaaaattggc atatatctgc tgtggatctt tcagaagaag cattagaagt agcgaaagaa
    10261 aatgctcaga aagaaggaac gaaaatcagt ttttatcatg gagatacatt aggaccagta
    10321 atggatcagc aatttgatgt gattatttct aatccgccgt acatcagtcg gaatgaatgg
    10381 gaactgatgg atgaaagtgt ccggtcattc gaacctaaaa tggccctttt tgctgagaac
    10441 gatggattag ctatttatga aaaaatagca gttgaagcac caagcgtact aaaatcaaat
    10501 ggaaagattt ttttagaaat cggatttcgc caaggtgaag cagtaaaaaa aatatttcag
    10561 caagcatttc cagataaaaa agtggcgata aaaaaggatc tttttggcaa tgaacgaatg
    10621 atccaagttt atggttgaag ttgtggatga aaaatttttt atttgttttt ttattataaa
    10681 aaaacactta tttgacaagg atgtaaacga ctttttggtt tatcaacaag tttatccact
    10741 attgacaaat agattatcca caaacagtgg aaaacttgag gataaactca gacaaaataa
    10801 gaatgtatga aaaaggggtt gtggataaac tgtggaaacg aaacgatatg atgaaacaga
    10861 attgcaagag gcagccgaag cactgaaaga aggagaactt gttgcttttc ctaccgaaac
    10921 agtatacgga ttgggcgcca acgctctttt accaaacgct gtcaagaaag tgttttctgt
    10981 aaaagggaga ccgcaagata atccattaat cgtccatgtc gcctcttttg aacaagtaaa
    11041 agagtatgtg gacaactttc atccggcagc agagaagatt gtgaagaact tctggcctgg
    11101 accattaact ttgatattta agatcaaaaa agacactttg ccctcagttg tgacgggagg
    11161 attatccaca gctgcatttc gtataccgga taacaaaaaa acactagaag taatcgaact
    11221 ttccggtgtt ccgcttgtcg gtccaagtgc aaatacgtcg ggaaaaccaa gtccaacgac
    11281 tgcagatcat gtatatcatg atctacaagg aaaaattacc gggattattg atgatggagc
    11341 tacgcgaatt ggtgtagaat ccactgttct tgatttaagt gatccaacag ccatgcctat
    11401 gattttacga ccaggtgcag taacgaaaga acaaatcgaa gcagtcatcg aatctcctgt
    11461 tgctattgat cagcatcttg taaaagaaaa tgaaacacca aaagcccctg ggatgaaata
    11521 caaacactac tcacccgata cacgtgttct gatggtcaga gatggcgatt ggtcaactgc
    11581 agttcaatgg gcaaaaaata agaagatccg tgcaggtgtc atagcaagcc ccgaaattgc
    11641 ggatcaagtg cggacagata ctgcagctgt ttatatgtat aacgacaact cagtagaagc
    11701 ggcggctaaa ggattgtttg caggtctaag aggattagat gaaccaacac ttggattaga
    11761 tttgattttt gtacaagtgt atccagaaac tggattgggg aatgcttaca tgaatcgctt
    11821 gaaaaaagca gcaggacaaa attattttga aaaatgatca attggttatt gtcgcattct
    11881 tttagtatga tacaatgcta tcaagtaaat cttctatcag tgaacgaaaa actaagcgta
    11941 actaatcgta atagaagaga tacgaatatt tggcaatttg tttttgatgg gaaaaataaa
    12001 attgtaggaa aataaaaagg agctgttgtt tgtggtagat tacaaaacgt ttgaccctga
    12061 tctttgggct gcgatcgcca aagaagaaga aagacaagaa cataatttgg agctgattgc
    12121 ctctgaaaac tttgtctccg aagcagtgat ggctgcacaa ggaagtatct tgacaaataa
    12181 atacgcagaa ggttatccag ggcatcgcta ttatggtggg tgtgaatttg tcgatatcgt
    12241 tgaaaattta gcgattgacc gtgcaaaaga attattcgga gcgaaatttg cgaacgtcca
    12301 gcctcactcc ggctctcaag ccaacacggc tgcttatctc gctttggtag aaccagggga
    12361 cacgatctta ggaatggatc tttccgcagg cggacatcta acacatggat caccagtcaa
    12421 cttcagtggc aaaacctatc actttgttgc ttacggtgtt gatccgacaa cagaagttat
    12481 tgattataat gtggtacgta ttttagctag aaaacatcaa ccaaaattga tcgtagcagg
    12541 tgccagtgct tacggacgaa cgatcgactt tgcgaaattc agagaaatcg cagatgaagt
    12601 aggcgcaaaa ctgatggtag atatggcaca tatcgcagga ttagtagcag caggacttca
    12661 tcctaatcca gttccttatg cagatatcac aacgactacc actcataaaa cattgcgtgg
    12721 ccctcgaggc ggcatgattt tgacaaacga tgaagcatta gcaaaaaaaa taaatagtgc
    12781 ggtatttcct ggtattcaag gcggccctct agagcatgtg attgcgggta aagcagtagc
    12841 attcaaagaa gcattagatc ctgcatttaa agaatatagt gaacaaatca tcgccaatgc
    12901 aaaagcaatg gtcaaagttt tcaatcaagc aattggaacc agagtcattt caggggcgac
    12961 agataatcat ctgatgttga tcgatgttcg cgagttaggt ataaatggaa aagaagcaga
    13021 gagcattttg gacagtgtga atatcactgt aaataaaaac tcgattcctt ttgaaacact
    13081 aagtccattc aaaacaagcg gtattcgaat cggcacgcca gcaatcacta ctcgcgggtt
    13141 caaagaagaa gatgctgtaa aagtggctga actagttgtc aaagcattac aagcaaaaga
    13201 tgataacgct caactagacg aagtcaaaac aggtgtacgc gaattaactg aaaaatttcc
    13261 gttgcataaa aaataaaaat ttaaattcca ttagtaaaca gaaaagttca atgttgggct
    13321 tttctgttta ttttttggga aaacggattc tttgacaata tcggtgatgc tctctagtat
    13381 atgaggtgta attttagtct tcacactaaa tctcagggag gaaaagtcat gactgatcgt
    13441 atctcgttag aaaaggaagc agtagcattc agtgatgcaa atgcgcctca tccgcgtatc
    13501 tatgaactag caccggaaga aggaagagtt ttactagaag aagtacaaga atcacctgtg
    13561 aacaaatatt ccgtcgatat tgaagacttg atggtggata ctggcgaatg gggaaaactc
    13621 aatattcgtc tcgtacgacc agaagggaat atggataaat taccagttat tttttatatt
    13681 catggagcag gctgggtttt tggcagtgct caaacccatg ataaattgat cagagaactt
    13741 gcggttcgta cgaattccgt cgttgtcttt ccagaatata gtcgatcacc agaagcaaaa
    13801 tatccaacag caattgaaca aaattatgct gtgttgcaac agctgaataa attagccaag
    13861 tcaaaaaatc tggatctcga gaatctaatc gttgcaggag attcagtagg tggtaacatg
    13921 gctacagtca tgacgatctt aacaaaagaa cgaaagggcc tcccaatatc caagcaattg
    13981 ctgtattatc cagtcacaaa cgctgatttt gatacagatt cctatcatga attcggagaa
    14041 aactattttt tgacaagaga aggaatgatg tggttttggg accaatatac gacgaatgaa
    14101 gaagagcgtg ctgaaatcac agcttcgcct ttgagagcaa atataaatga actaaatgat
    14161 cttccttcag caatgatttt aacgggagaa gcggatgtat taagagatga aggtgaggct
    14221 tatgcgcgca aacttcgtga tgcaggggtt cctgttacac aagtacgctt ccaaggaatc
    14281 atccatgatt ttgtcatggt aaatgctatg gatcagacaa aggcaacgcg tgcagcaatg
    14341 gcattatcta cttcttggat caagaaaaaa gaataagctt tgcttgtccg caggaaaact
    14401 tcttttcaaa aacggatttt cttaggaaaa atggcgagtt tgactagtca atcacttgat
    14461 tttgttttac aattaaaaag aaacgacaga acaaaggaga gcttaaaaaa tgggcaaatt
    14521 tcaagtgatc gatcatccat tgattcaaca caaactaact attattcggg acaaaaactg
    14581 cggaacaaag gtttttcgtg aagtagtcga tgaaatcgct atgttgatgg catatgaagt
    14641 atcacgggat atgccattgg aagacatcgt gatcgaaaca ccgatcactg aaacaacaca
    14701 aaaaacgttg tctggtaaaa aagtagcgat tattccaatc ttgcgcgcag gtattggaat
    14761 ggttgacggt atcttggaat tgattccagc tgctaaagtg ggacatatcg gtctttatcg
    14821 agatgaagaa acattggaac cacatgaata tttcgtcaaa cttccagaag acattgatgc
    14881 acgtcagttg tttgtagtag atccaatgtt ggcaacaggc ggatcagcaa tcatggcaat
    14941 cgatgcattg aaagaacgtg gcgcgtcgaa cattaaattt gtttgtttgg tagcagcacc
    15001 tgagggagta aaagctttgc aagaagcgca tcctgatatt gacatctata cagcttctct
    15061 agatgaaaaa ttgaatgaac atggttatat cgttcctgga ctaggggatg cgggtgaccg
    15121 tttgtttggt acaaaataag taaatacgaa aaagggtgtg acacaacttt tgtcacatcc
    15181 ttttttggtc atttttaacg tttgatgcag aacagctttt tcacagcttt cgtttgcata
    15241 taaaaaaacc atcccaaaaa tgcagagatg gtcaaattca aatctttttt agttctttgt
    15301 agttaacgat tactacaccc ttactttgcg tcatcaagtt cgcattttct gcagtttcat
    15361 caatctcaac aacagcggaa tttttcaatt gctttgaaat cgttccagtt tgcgacttgc
    15421 cgtgaattgt gaacattacg tgagtattga tcttgaattt ttcaccaatt tccgatggta
    15481 cgatagttgt atcaaatttt ccaaaagttg ccattttcat aaaacctgcc tttcacatca
    15541 attataccac gttttatgaa aaatatcatg aaattaaact aattttccat cggactcttt
    15601 acaatcagcc aaatagagta tataatttct tttggataag gccagcttag ctcagttggt
    15661 agagcaacgc actcgtaacg cgtaggtcgt aggttcgatc cctatagctg gcattaaaag
    15721 agaagacaga tatatcaagg atttttaatc cattgataca tctgtctttt tgtttaaagg
    15781 aaataaagta tgctttctta gcaatagagc atgtattatt tctataaaaa tttaggagaa
    15841 taaaacgact agcaatatag ctgtgaaatc aaatatcaca tgaactgccc aacttgtaat
    15901 gatcgagttg cttttaatgg cagcttggtt gaataacaat cttgccaccc cttgtatcaa
    15961 aaggatgtgt gctacagtgt gtaaaatatt tccattatca taagtactgt aatggataaa
    16021 tccaaagatg attgctgaca agaaactagc gacaggaatg ctgaacttac tagaaaaaat
    16081 agcgagaaaa tacactgaga acaattcttc tccaagtatc atgataggca aaataataaa
    16141 taataacggg ctgaaagcct catttattgg attgcctgtt aaaccccatt tcaaaatttg
    16201 ttgcaaaaat accgagactg cggcactgat tactacattg ataaaaaaat atttggctat
    16261 atttttgaca ggagcgacag gtttcgagaa catttttttc gtatcttttt taccaaaaac
    16321 aagtgtaaat gctataaatc cagtgatagt taaagaaaaa atgctaataa tggatggcgc
    16381 aaatagcaac cctaacaaac cgagtacaac gacaatagtg cccaatatcc cttttttaag
    16441 ttctattttt tttcctaaaa tgttattcat gataataact cctactattc agtttttgaa
    16501 gatagagtca gtatacatga agagttaaaa taggcaatgt ttttttatgg atatttgtat
    16561 tgctaaaaat ttatcttata tattcatttt gattatacta cttttatttg taaaaatttt
    16621 ctagcaaaac caagtgcttg caactgatta agcgtacaat tttttagatg ttccgagcgt
    16681 acttcaatca tttcaaagga gttgctactt aaatcgatat tttttaaatc agtatcaaaa
    16741 aagctagcat tttctaaatc attagtagaa aacattattt tagtgaggga acagtctaca
    16801 aaatctgcgc ttgataagac agagtgctca aaggtacttt cttttatgtt tgaaccagat
    16861 aaatttacat agtccatttt acaattctcg aataacgtgt tcccaatata actttccatc
    16921 aatttagtac ctgttaattt acaattgaca aatttcacgc gatgcaacat gctttttgtc
    16981 aatatacagc ctgaaaaatc acagttttcg aatatacagt cggtaatctc cattctttga
    17041 aaatcattgg aagcaaaaaa tgcatacttg aaaatagatt tgtaaataat tggatggcag
    17101 attatttggt tttcgatgac ctctttttgt gaaaggatat tatacagttc aaaatcttcg
    17161 ttttcataga tatgaaaaaa ggatccttta ttcaagcttg cttcattaac tttaggaaaa
    17221 atcatattta attacctcca ttttcataaa gtcatattaa caaaagctaa accaacttac
    17281 attagcagaa aatgagatag gttttcgcct ttttcgataa cgatagtgtc gctatctctt
    17341 gaaacgttct tagaattgcc ctgagagaca caaattaggc gtaattctaa taaaaatctt
    17401 agaataaaaa tttttactga tttaatttag aatgttttta aaataaaaat cgttgccata
    17461 acagtgtatt gaaaggtttt aacaatccag taatgattta aaaaaatcta gatgtagcgt
    17521 atactttgtt tatagaatgt agtgggaaga aatatatttt tgggaggaaa tcgtgatgtc
    17581 taaaagtgaa gaaatagaaa acatggtact aaaattatat gatctcgttt taaatccaga
    17641 gatcaaagaa aaggaacgta tacttttgat agacgcaaag acaggcttag aaaaaggtca
    17701 gtattatcct aaagtcataa ataatttgga acgtagttta cgtccgttag caatcagggg
    17761 agaattgtct aaaccagtat caccgtttta tatggagata tcaacaatag gaaaatttga
    17821 aaaagaatta ggtcgaggaa tggcttcggc accgattact tttgggcatt tataaaaacg
    17881 agttattcat ctcaatgaat taaaaagtat agtcaatctc ataatgcagg ctaggttcat
    17941 ttatttgaac agagtctgta tttttttatt ctaaataaag aaacagatgt ttctttatat
    18001 tgtattttat cctttaatta acacaaaaac agctagtata aactaataat gttaattaaa
    18061 agtgtatttt atgaaactat cgttatttat taatgttgca tttttgtaag ttaaaaattg
    18121 gaaatataga ttgaaaaaat gaaagcaata agtaaaataa aatgaatata tgcgaaaaaa
    18181 taatttac
//
LOCUS       DARBDV010000063        17369 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000063 DARBDV010000000
VERSION     DARBDV010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.063"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            34..294
                     /locus_tag="SCM56_001963"
     CDS             34..294
                     /locus_tag="SCM56_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110723.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(656..895)
                     /locus_tag="SCM56_001964"
     CDS             complement(656..895)
                     /locus_tag="SCM56_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110724.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            1181..1495
                     /locus_tag="SCM56_001965"
     CDS             1181..1495
                     /locus_tag="SCM56_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110725.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            complement(1929..2295)
                     /gene="ssrA"
                     /locus_tag="SCM56_001966"
     tmRNA           complement(1929..2295)
                     /gene="ssrA"
                     /locus_tag="SCM56_001966"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2466..3239)
                     /locus_tag="SCM56_001967"
     CDS             complement(2466..3239)
                     /locus_tag="SCM56_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG1110726.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            3383..3733
                     /locus_tag="SCM56_001968"
     CDS             3383..3733
                     /locus_tag="SCM56_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HEG1110727.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            3714..4430
                     /locus_tag="SCM56_001969"
     CDS             3714..4430
                     /locus_tag="SCM56_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1110728.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            4430..5878
                     /locus_tag="SCM56_001970"
     CDS             4430..5878
                     /locus_tag="SCM56_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG1110729.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(5948..6457)
                     /locus_tag="SCM56_001971"
     CDS             complement(5948..6457)
                     /locus_tag="SCM56_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110730.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(6447..7172)
                     /locus_tag="SCM56_001972"
     CDS             complement(6447..7172)
                     /locus_tag="SCM56_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110731.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(7395..9515)
                     /locus_tag="SCM56_001973"
     CDS             complement(7395..9515)
                     /locus_tag="SCM56_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEG1110732.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            9745..10923
                     /locus_tag="SCM56_001974"
     CDS             9745..10923
                     /locus_tag="SCM56_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEG1110733.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            10939..11697
                     /gene="aroD"
                     /locus_tag="SCM56_001975"
     CDS             10939..11697
                     /gene="aroD"
                     /locus_tag="SCM56_001975"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEG1110734.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            11720..12205
                     /gene="ybaK"
                     /locus_tag="SCM56_001976"
     CDS             11720..12205
                     /gene="ybaK"
                     /locus_tag="SCM56_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEG1110735.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(12276..13529)
                     /locus_tag="SCM56_001977"
     CDS             complement(12276..13529)
                     /locus_tag="SCM56_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEG1110736.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            13647..14996
                     /gene="gdhA"
                     /locus_tag="SCM56_001978"
     CDS             13647..14996
                     /gene="gdhA"
                     /locus_tag="SCM56_001978"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEG1110737.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            15108..16454
                     /locus_tag="SCM56_001979"
     CDS             15108..16454
                     /locus_tag="SCM56_001979"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEG1110738.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(16762..17148)
                     /locus_tag="SCM56_001980"
     CDS             complement(16762..17148)
                     /locus_tag="SCM56_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEG1110739.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(17197..>17369)
                     /locus_tag="SCM56_001981"
     CDS             complement(17197..>17369)
                     /locus_tag="SCM56_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEG1110740.1"
                     /translation="VETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQR
                     RFEGTLRYSLGY"
BASE COUNT         5708 a         3034 c         3452 g         5175 t
ORIGIN      
        1 gaataatgaa aaaacattaa ggatggatga aatatgaata acgaaactat cattttagga
       61 aaaaaatata tttgccaacc tattggcttg aaacaaaaag tggttggaga agtaatcagt
      121 aaattagaaa attgtgttgt gatctgcgta gacgaatatc atgacgtcga tcgcaatgaa
      181 atattagaaa aatgcggaaa agtagtggca aaatatactc acgtttatga gttggaaaca
      241 acagaatatc tatataacta tagcaacaaa tatgaacata ctttagtttc ataaagattt
      301 aaataaagtg ccttcaaaag cgagaagacc aagcttttga aggcacttta tttagttggc
      361 agtgattgtt catgattttt gatttattca aaaggttcgc ctagagaatc tgtctctgta
      421 acgaggcctg gtggagaaac tcgataaaaa cctatactgc cagaactgtc ctattttcgg
      481 atagacaaac tagtagtttt aaaaagatca tccgatccaa tcatttcaaa aaatgcaaaa
      541 ctgatatcag gatcatttag ctcaggcttg ttatcgtaaa gcatttgtaa aggttgcatt
      601 tagttaggag taaacgcgaa actatcatca ttatttgctg aagaagatga agaagctaat
      661 tcagagtttg tttgaaataa aggagacgtg ctgacagttt ctttaccctg ttttttgcag
      721 aggtagaaac ttttatatcg gaagtttcta ccggagaatt tttctcattt gtagaaattg
      781 ttgaatcgat actattcgaa atttgcgtat ctgaaggtat gttctttgat gcagaagtcc
      841 cacaagcagt aagtaacaat aaatttgata aactgataaa cagaattctt ttcattatgt
      901 acacactcct tatagtatcg tttagcatac acctatgctc agtattaggc aaaatcaatg
      961 caaaactatc aagtctttta tagtatctat tagtcttagt aatacatata tggagagggt
     1021 gatatcccag ttcaaaccag atacattggt taccatctgt attaacgatt atgcctctaa
     1081 acgctttttt tatgaaaaaa tttataagtg aaaacgtagg tatgatatta tctagaggta
     1141 atttttttgg gtatttaaat agttgctagg aggatagctt ttggattttt acagtaattt
     1201 tatcttaata atagcaatat tattacttct aaatatttgg ttttttgata agagtagaaa
     1261 tgcagggata ggttttagaa ccaaacgaag tacgtcttct gaaaaaaagt gggtatattc
     1321 acagactatt ttttacggag gggttatttc aatcagttta ctttcttcaa cactttattc
     1381 ttttaatgtt atagacgttt ccatgtcaaa ttttatatct attattggaa tccttatttc
     1441 tgcaattatt acacaactac ttcttgtgtt tgaagagaag tcaaaaaata attaaagcaa
     1501 ttatgccttt tagtacagga attattactt cttacaaatg tatagcgaat gtgaagcctg
     1561 ctgttttaat tgttctagaa aaacttttga gtgaacaatt attttatgtt aggatgaatt
     1621 aatttctcta gagtttataa ttaaaaaact tatttgaagg aacatgctta aaccataatt
     1681 gtgtttttcc ttccatcagc ataaaagttc acctcaaaaa gagtgtacga acaaaagttc
     1741 tttttgtaaa ccttaaaata gattttcctg aatacgtatt ggggatttct aaatgtttga
     1801 atgttttttt gaatgcaaaa tcaatgcaaa aaattttgta tttctctact tttctctcct
     1861 tgaccaaaat aaaaaaacgt tgatttaaca acgtttttca tacatgttta tctgtctcaa
     1921 ccatacatat atggagacgg cgggagtcga acccgcgtcc aaacacatcg ccacttaaaa
     1981 atctacgttc atagtcaact catttaagga ttcactttac agcttgccga gtgacaggca
     2041 ttctgtatcg ctagtctgat gttctctttt tcaacttaca gacggaaagt tgaaacgtat
     2101 cccactaaag ttaggaccct tactcgagca catgggcgat gccgagagga tctacgctaa
     2161 ctgtttttag gcagctaaag cgtaagagtt gttttcgttt ttagcagtta tatttaactg
     2221 taacgttttt acgtagacgt aacctacgaa acgcaattca agctcgactg tgcctgtcga
     2281 atccgtaacg tccccgttag ctaacaaggg ttacgtgtat ttttcataca aaattagtat
     2341 agcatatatt gggataaatt caataaaaaa gcgaatcata tcccattatt tatataataa
     2401 ctgccccttt cttaagaaaa aataagcgat tttcttaaga aaagaggtag cttcaaaaaa
     2461 agatcttaaa tcagatcaaa atacttcaat gcgtggacaa ttccgccttc tgtatttttc
     2521 tttgtgataa aggtagaaag ttcttttaat ccgtcatagg cgttgcccat agcgattttg
     2581 ttgtcgcaag cttccaacaa ggcaaaatca ttcggaccat cgccaaatcc aaaagtagga
     2641 actttttcca gctgaagttc tttttgcaga cgtttgacac ctgccccttt tgagattcct
     2701 ttcttcactg tatcgatcga gtaaggacca ttccgataaa aagttagctc aggaaaatgt
     2761 tcgtgatagt attcgtcacc tgattgagat aagatcagca acatatttac tgtgttttct
     2821 ttaaagccta aaggatcaat ttccggaaca tctgaatgaa tgaagtcgta agcattgatc
     2881 attgtttggc tatgaccggt acaccagatt ttctgatcgt tatagtaaga aagttcatga
     2941 ccgttcaact gtacatgatc atacattttt tgacattctt ctgaagaaaa tgtttgagaa
     3001 tagatttctt cgccgtctac tcgaatgaaa gaaccattca tggtgaccgc agaagtaatg
     3061 cccgcatctt ccatgataga gtggatttct gcttctgtac gtcctgtagc gatgacgggg
     3121 agaacattgt tttcttttaa tgcgttcata gcttttgcca cttcgggtgt gatttttgat
     3181 ttcgcatcta gtaatgttcc atcaagatca aagaaagtga ttgcacgata atttgacaca
     3241 aataaaactc cttttttatt ggaataagtt tatcttaaca aagaatgaac aaaagacaaa
     3301 cctttcgtga agaattcctg aacatattgg ttcagtcaac aatttagagt atactataag
     3361 aagagaattt aggagggaaa gaatgatttt tttcagtatt ggtatcttat tcttaataat
     3421 tggcagtctt tttcggattc tgccctcaaa aggaaatctg cctttttatg ggtatcattc
     3481 gcctcttgcc gcaaaaacag acgctcattg gcggctggct caaaagacaa gcggaaactg
     3541 gttttttctt atgggcttac tgatggcatt gatcggctat tatttaaaga catctgggca
     3601 tacgaattat tttctgattg aaatgcttct gctagtattt ccaattatgc ctatttttat
     3661 tatgacagaa aagaaattac agaaatatga cctggaaact ggagggaacg ataatgaata
     3721 ttttaatgat tgaagataat caatctgttt ctgaaatgat gcagatgttt ttcttaaatg
     3781 aaggttggga agcaactttc aagtatgatg gaaaagaagg gctcgatgcc tttttagaaa
     3841 acccacaaca ttgggatatg attacactag acctgaattt gcccacactg gacggtatgc
     3901 tagtaggaag agagatccgc aaagtttcaa gtactgtgcc gattatcatg ctgacagcta
     3961 gagattctga aagcgatcag gtgatcggct tagagatggg ggcagatgat tatgtcacaa
     4021 aacctttcag tcctctgacg cttattgctc gaatgaaagc tttgcatcgc cgtagtgagt
     4081 tggcagaagc aagagaagaa gcagcagttt cagatgatca ttttgatatc gagactagtc
     4141 atttcaagat gaatacgaaa acacgagaag cgtatctgga caaccggcca attgaaggac
     4201 tgacgcctaa agagttcgat ctattgttta ctttagcaaa aaaaccacgt caagtattct
     4261 ctcgggagca gttgcttgaa ttagtctggg attatcagta ctttggtgat gaacggacag
     4321 tggacgccca tatcaagaaa ttacgacaaa aaatcgaaaa ggtcggacca caagtcatcc
     4381 aaactgtttg gggagtcggc tacaaattcg atgattctgg tgttgcctaa tgcgttattt
     4441 gtatcagcaa ctcttagctt tttgcggaat ggtcgcgttg atcttattga tcgttggagt
     4501 ttcttttaca caactgacaa aacaaacgat tgaagaaaac aattaccagc aactgtttgg
     4561 atatgctgaa tcagttgaaa agacaacgca aacctatgcc gatacgctcc cgcagtttag
     4621 tcaagatcag gcgtttcaaa atgcattgct gctgacagag caagtactga cagaacaaaa
     4681 tgtgaacttt atatttatcg ataaaaacga acgtgtcatt tatccgacta atacagcttt
     4741 actgaatttt accttaacag ctgatcaatg gaaaaatctg aggaatggac gtcaagaaaa
     4801 attcacatcg aataaaaata ttttaggaca agcacaggcg acctcttacg cacttgtgcc
     4861 atttaatttg aatcatgaat tctatggagc acttgtagtt agtcaacctg cgcgaaatat
     4921 cgataacagc gttcgtccaa ttacactgaa cctgttcaaa ggatttattt tttccagtat
     4981 cgttgcagtt attgccagct atgcttttgc agcattccaa gttaagcgta tcaatcggtt
     5041 aaggaatgcg acgaaagaag tgacgaatgg taattttgat gtacagctcc ccgtacatga
     5101 caaagatgaa tttgatgagt tagcagatga tttcaataaa atgacgaatt cattgaaaga
     5161 atctcaagca gaaattgaag aacaagaaaa tcgtcgaaga caatttatgg cagatgcttc
     5221 tcacgagatg agaacacctc ttacgacaat caacggactt ctggaagggt tagaatacaa
     5281 tgcaatccct gaaaatcagc gagaaaacgc gattaaattg atgaaaaatg aaacagagcg
     5341 tctgatccgt ttggtgaatg aaaacttgga ttatgaaaaa atccgtacga atcaaatcag
     5401 tatggtcatt aaaaaattca atggaacaga aacattgaaa aatattgttg cacagttaga
     5461 agctaaagca gaagcagcag gagatacatt gacattgaaa gccgttgatg atattgatgt
     5521 ttacgctgat tatgaccgat ttgtccaaat catggtcaat attattcaaa acgcgataca
     5581 gtttactgaa aacggccaaa tcacgattac tttagaaaaa ggctatcttg aaacgatcat
     5641 cacgattgaa gatacgggaa ttggaatgtc tgaacaacaa atgaaaagta tctgggatcg
     5701 ttattataaa gtagatcctt caagaaaaaa tacaaaatat ggtgaatcag gattaggttt
     5761 accaatcgtg cagcaacttg tacgcttgca taaaggaaaa ttagaagtag aaagcgaact
     5821 aggaaaagga acaaaattcg ctgttcgttt gcctgacgta gaaatcaaaa aagagtaaaa
     5881 aacaagcggt ataaaagatc atgaatcgat cttctatacc gcttgttttt actataaaat
     5941 gattgattta gcccgcgggc tctaaacgat caatataagg actttgagca atagtagcat
     6001 catgagtaaa gagtaagata ccttgatttt cttcatgagc taaataagaa agcaagtgga
     6061 gtagctcttg gatctccaat acactaagtt catcaaatgt atcttcaata acaatattgt
     6121 ttcgcttgga aagcaaggca tgaagtaatt gcagctttac tttttcaaaa gacgtcaact
     6181 cagctgccgc acaattaagc aaggaaggag agatatcaag tttttcttga taggccaaac
     6241 gatcttgttt gatttctttt tccggtacat ccaacaggac atggtcttta agattcagat
     6301 aaggaaacat cggccaattt ttttggatga tcgcaaaatt atccagtttt gcaacaagaa
     6361 cctctttttt caagtgctcc aatattttat gctgtttttc gagaggacac aaaataacgg
     6421 tcaagtgttc agcatgaatc aaatattcag gtttcattac gtccaggcct cctactttgc
     6481 aaatgatgga taaaaaaagt gaaagggaat agactaagaa tgagacagca tccactgagc
     6541 ccaataagag tttgggcaaa tcccttaagt atatctgtga aaaaaagatc attttggaaa
     6601 tcgatggtaa aaaaagtctg ttgatccatc ggaattgtaa gtccgcttac cgaagtcaag
     6661 atatcacgga tatctggcag ttcagtagtc tttaagacaa gaaaattgat cctttgaaga
     6721 agcgcttcat aaactgactg gaagcttacg atccacaata aaaccaagat acaacaaaga
     6781 aagagaggaa agaacagctg ccagaaaata aaaaggaata atttggtttt agaaagacca
     6841 gtcaggcgcc aattgatcaa ttcctgcttg ttttgcctga ctgaatagta aaagaaaaag
     6901 ataaaaagga aggcaaatag aaaaaataaa ctgaatatgc cttttatata aaatcggcta
     6961 gctaattcta aagccggaag aagctctgta gatgcagaag cgccaataaa tgaaagccga
     7021 tcttggactt gattggctaa agccgattgc aaatggtaaa gagtcaggca tgctatcaat
     7081 aaaaaagcag aaaagcttga aaataaacaa tagagtattg tctgcttttt gtaataaatt
     7141 aatcgagcag tagcatagcg gattattttc atacttttca cctctataaa aagtatagaa
     7201 aatgaatatg aataaagtat gaaattcctt tagtgaaatc acgaattaaa accagaaaat
     7261 attttgtaaa aagcaagagg ttgtgaagaa gctgccctat tctattaccg tagatgcaag
     7321 cttttgaaca ctgtttattc gaaaagagga gcagcgacaa taattttgtc gctgctcctt
     7381 ttaaatttat tttgtcattc ggaagaactt gtggtcgttc cgtgaagttc ttggtacgtt
     7441 tgcgttttat aaagatcaac agttgattgt ccgttgtgtt cactaaagac actagttgac
     7501 ttgtcaccaa gagatttttc agcttctttt aatttctcta agccattctt gtagtcataa
     7561 tcactggtat caattgggtc taatccacta ttcgtataga accgaagaag atcaccatta
     7621 ttgatttggt cagaaacagc taactgttta tcaacttttt ctttcagtga atcaatttca
     7681 ttctgtactt cctcagtagg ttcagtaata gcttcaccag tttcgttact atagatcgta
     7741 ccaccgtagt aagtatattt tggtgtaaca aaatcaccat cacggaaaga aacaagctgt
     7801 tcatgatctt tcgagaatag atcttgtcct aactgaatat aattttttgt atctacacct
     7861 agtaagtgaa gtaatgtagg caaggcatca acttgtccgc cgtaagtgtg attgattccg
     7921 ccctgtgttt gtccagggat atggatcata taagggacac gctgcatttg tgcattgtcg
     7981 tagtttgtcc aagtagaact tgtttttcca actaattcag ctagattttt atttcttgaa
     8041 ttcgagacac cgtaatggtc accgtaaagg acgatgactg aattatcata caagccgctt
     8101 tctttcaaat aattgaagaa ttcttgtacg gcggtatcta aataattggc tgtcgcaaag
     8161 taaccgttga tcgtttcatc atttgtattg gctaacggga atcctgcttc gtcattggtg
     8221 aattttgaat aaggataatg gttagataca gcaatgaatt ttgaataaaa tggttgttgc
     8281 agacgttcca agtattgtac ggattgttgg aaaaatggtt tatcatgcaa cccgtattgg
     8341 aacgaattgt catcattaac atcataataa gaagaatcaa agaaataatc atatcctaaa
     8401 ttcttgtatg tctcgtttcg attccagaat gtaccagcgt tcccatggaa taccgcacta
     8461 gtgtaacctt gtgtttgact taaaatatca ggtgcagctt ggaaagtatt ttttccgcct
     8521 aattgtgtga acaaggagcc ttgattcaag ccaaacaatg aattttccat cagcgtttcc
     8581 gcatcgcttg tttttcctgc ttttacttga tggaaaaagt tgtcaaaact gaacgtgctg
     8641 ttgctgtggt aaagactatt gatgaaaggt gtaacttcat gctggagacc attctcatct
     8701 gtcaatttat aatcgatcag gaattgctgg aagctttcta aatggatata aatcacattt
     8761 tttcctttag cgagcccgaa caaatcgtca tttggtgctg cataatgttc ttttacataa
     8821 ttttctactt ctttcatatc attgggactt gcttctgccc gcacttggtt tgtctggtaa
     8881 gtttgaacac cgtcataaac catgaaagca ttcaagccaa gatatttgac caaataatca
     8941 cgagagaatg ttcgagtcag caattctgga cgatcagctt ccgccataaa aagatttcct
     9001 gagaaaatca taactgctaa tgtagagatc gcaaaagcca ttcttgctcg gatcgggcga
     9061 gggtccaact tgattttttt tgcaatcagc agaacagtca agataataaa atcgatccaa
     9121 tagatcacat catatgggcg aaacagtcga atggcacttt ctcccagacc actcgctact
     9181 ttcccagcac ctaaaatcgt gttgatcgta atgaagtccg tgaattctcg ataataagtg
     9241 acattcgaaa aaacaagcaa tgtcaaaaga aaataaatga ccatcatcgt gatataagca
     9301 actctagggc gtctgatata aagagcaatt ccaagtaata acaatgtagt tgcaataggg
     9361 ttgataaaca agataaagta ttgaatgcca ctttcgattc cgagatggaa atcaactaaa
     9421 taggcaaaaa tcgttttcaa ccaaagtaaa acgactagta aagaaaaaaa tccgagcctc
     9481 ttattcaaaa aggcaggtgt ttttatacat ttcaagataa tcttcctttc tgcacttctt
     9541 aatcacagaa gtgtcatgaa accatgttac acagctcact tattctcatt tattttacta
     9601 gtatgacaat atagtgtcaa ttgaaaccag aagtttatgc tgtttcatta aggttttctt
     9661 taagcttcat gaaaaaatga gcagtaagac aagtcctatg ttttttgtta tacttgggaa
     9721 gagaaagcta aagcaggtga aggaatgaaa gtaaaagtaa aaaaaagagc aagcaatcga
     9781 ttgaaaaaag gatatccact gatccaaaaa gaagaccttg ctcatcccca aaaagaaacc
     9841 acgcaatggg tggaattagt tgattcgcaa aaccaattta tcgcaaaagg atatctagga
     9901 aagcagaata agggaatcgg ttgggtattg agccagcaga acatcccatt tgatcaatct
     9961 ttttttgaag aattttttgt gaagtcaaaa gaaaaaagaa ctttcttttt ttcagatgat
    10021 tctactaccg cttttcgtat attcaatgga gaaggagacg gaatcggcgg tctgattgtt
    10081 gattattatg caggttatgc tgttttttct tggtataacg aaacgttata tgcacatcgt
    10141 gaattattga ttcaagcttt tcgtgaaatc tacccggaaa tcgtaggagg ctatgagaaa
    10201 attcgatttg aatcagtaaa tctgcctgag agccagttca tttacggaaa agaggcaccg
    10261 gaacccttga tcgtaaaaga aaacggagta aaatttgccg tttatttaaa tgaaggatta
    10321 atgactggga tctttttaga ccaaaaagaa gtacggggat cattagcaga aggtttcgct
    10381 tctgggaaaa cagttttgaa catgtttagt tataccggcg ccttttctgt tgctgccgca
    10441 atgggagggg caatcgaaac aacaagtgtc gatctagcaa aacgcagttt aaaaaaaaca
    10501 caggaacagt ttgaagtgaa tggtttggat gtcgatcagc aaaaaatcat tgttatggac
    10561 gtattcgagt atttcaaata cgccaaaaga aaacaatttt tatatgacat gattgtattg
    10621 gatccaccaa gttttgccag aaataagaaa aaagtctttc gcgtagcaaa agattatggg
    10681 gaactggtca aggatagtct ggatatttta gcagaagacg ggatacttat tgcgtctaca
    10741 aatgctgcga atgtctcgat agaacaattc caaacaatga ttgaagagga attccatgcc
    10801 aaaaatgttt cttttgaaca acttgcttta tatcgtctgc caaaagattt ccagacgacg
    10861 gatacctttt tagaaggcaa ttatctgaaa gtgtttgttt atcaaattaa aaataaccat
    10921 tagaaggagc gatgcagaat ggtttgggta aagggattga atataggaag cggtagacca
    10981 aagatttgtg cgcctcttat cggggccgtc cgagaggaag tattacgtga agcagttctt
    11041 gcacgtgaag caggggccga tttagtcgaa tggcgagtcg atcattatcg tgaagttttt
    11101 tcttttgatg aagtagcaga aacattggtt cttatccatg agatgcttga tggactgccg
    11161 ttattattta cgtttcgtac cttagctgaa ggcggagaac aagacatatc gatccgtcag
    11221 tatcgcgacc tttatgaaca tgtgatacac acaggattag tagatatggt cgatatcgag
    11281 ttgttcaaag tagaaagtct aggaaaaaga ctgatagagg aaatcaagtc tttaaacatt
    11341 cctttaatta tcagtagtca tgattttaaa gaaacaccag cagaccctgt cttgctttac
    11401 cgattgaatg ttatggaaca ttttggagcc gatatcggaa aactggcagt aactcccaat
    11461 aatgaacgag atgttttgcg gttgatggag ttgactagac gggcaaatgc ttttgtctcc
    11521 atgcctatta ttacgatgtc catgggaaat cttggtaaaa tttctcgtct ggctggcgga
    11581 acgacgggtt ccgttatgac atttggttcc ttaagcagcg attctacttc tgctccagga
    11641 caattaccgg tcaaagcatt gaagcaaatt attcaattat taaatgaacc gttgtagaac
    11701 aagtttgagg tgatattttt tggcaaagaa aaaaatagta aaaacgaatg caatccgtat
    11761 ggtcgaacaa aaaaagattc cctatacaga gcacgaatat gagtgggacg aaagccacct
    11821 aagtgcgtca tcggtcgctg aacagcttcc agaaagccaa tctcgtatat ttaaaacact
    11881 ggttgctgtg ggcaatgtga cggggcctct tgttgctgtg ataccgggag aagccgaatt
    11941 gaatctaaaa aaattagcaa aagtttcagg gaataaaaaa gtagaaatgc tccatttaaa
    12001 agatttggaa gcaacgaccg gatacatacg tggtggctgc tcgcctatag gtatgaaaaa
    12061 actatttcct acttatttag atcaaattgc agaaagctat gaacaaatca ttgtctcggc
    12121 tggcagaaga gggctacaga tggagttagc acctcaagat atctgtgcat taacgagcgg
    12181 acaatttgca gatataaaac aataaatcat acgtataaaa gaaagaagga ggccgggatg
    12241 ttgtttgtcc agtctccttc tttctttttt atacattatg ctctttgccc ggcattcatt
    12301 cctgctacat gccctgtagc gaaagccgct gtgatattaa agccgccagt ataaccattg
    12361 acatccagta attctcccgc aaaatacaga ccatcaatga ttttactctc caatgtttta
    12421 ggataaattt cctttagaga aacccctccg ccagttacaa atgatttctc aataggaaaa
    12481 gttttgttga ttgatagctt aaactctttg catagttgaa caaaatcttg aatttgtttt
    12541 tgagaggctt gttgtcctgt cagatgatcc atttcaagcc gctcaagaaa gaaaactaac
    12601 aatcgctcag gcaatatacc atgccaagca ttgaccaagt tctttttggt tgctttactt
    12661 ttttctgtca atatatgaat aagctcttgt ttggtttgtg tagggaaaca atccagagaa
    12721 agtgtcactg gttctcccgt tttctctaat tctttattga caaaacttga acaacgcagt
    12781 gcagccggac cagaaatacc gaaatgggtg aaaagtaaat ccatcgtatg ttcagtcaat
    12841 acacgacctt ttttatttaa aacgcgtaaa gtgatgtctt gtaaagaaag cccttgaagt
    12901 gttttctcct gaatatacgg ttcatccgat atcaaggggg actctgtcgc ataaagaggc
    12961 gtcaccgtgt gacccactcg cttcacgatc ttgtacccat ctccagtaga accagtagat
    13021 ggataagtcc gtccaccagt tgttaagata atggcaggag catggaactc ttcgaattcc
    13081 gtgcgtatac cgtaaatttt atgttctttg tggatcaatt tctctactcg agtagagaat
    13141 agaagcgtca catctagttc tttcagacga ttgagcaatg cttcgataat cgtttttgat
    13201 ttatttgtta caggaaacat ccgtccatga tcctcttctt ttaaatgtac gccttgtgat
    13261 tcaaagaagt tcatgatatc gaaattattc cattgcgcaa acgtactata taaaaacttt
    13321 ccatttccag gaatatgtgc aatcaaatca tcaacaggac gattgttcgt aacattgcac
    13381 cgaccaccac ctgtcatcag tagttttttt cctgcttttt tgtttttttc gatcaagagt
    13441 acgcgtgcgc cttgttcagc tgcactgatc gctgccatca tcccgcttgt tccagcgccg
    13501 actacgatca catcatacag tttttccatt ccctcacctc aaggaaagtg tagcatatct
    13561 gaagaaagaa aacagaaaaa aaatgttttg agccaaaaga gaaaatgagc taaaatgagg
    13621 gaagataagc aaaggagcgt ggaacgatga caaacgcaac agaatatgtt caagcaattc
    13681 aagaaaagct acatcaaaag gatcaagggc aagaagaatt tttacaagca atcgatgaat
    13741 tcatgcccac ggttatgtct tttttggaca ctcatcccga atatatcgaa aagaatatct
    13801 tagaactttt aacggaacca gaaagagtta ttcaatttcg agtgccttgg caagatgatg
    13861 aaggaaactg gcgagtaaat agagggtacc gtatccaata caactcagct atcggccctt
    13921 ataaaggtgg attgcgcttc catcctagtg tcaatctaag tatcttgaaa tttttggctt
    13981 ttgaacagat tttcaagaat agtttaaccg gtctgccaat cggtggagga aaaggcggaa
    14041 gtgacttcga tcctaaagga aaatctgata atgagatcat gcgtttttgt caaagtttca
    14101 tgctggaatt gtcaaaacat atcggcccat cgattgatgt tcctgcggga gatatcggtg
    14161 taggagctag agaaatcggc tatttgttcg gcgaatataa acgattgaaa aaatatgata
    14221 ctggtgtgtt gacaggtaaa cctttggatt tttggggaag taagatacgt acggaagcta
    14281 ctggttatgg attagtgtat tatgtcaaac atctattgaa cgaagaaaaa gattcattta
    14341 atcaaaaaac agtttttgtt tccggaagcg ggaatgtagc catttatgca atcgaaaaag
    14401 tgcaagagct aggcgggaaa gttgttacat gttcagattc tagcgggtac atctatgatc
    14461 cagaaggaat agatgtgaaa ctattgaaag aagtcaaaga aatcaataga caacgtttga
    14521 cggaatacgc aaaccaacgt ccttctgcta tttatcatgc tggagaatct gtttggacat
    14581 tgaaagaaaa agcggatatc gctttgccat gtgctactca aaatgaaata gacgaaagct
    14641 tggccgaaat cttagtagaa aatgggatca aaatcgtagc agaaggcgct aatatgccat
    14701 gtacagtaaa agctgtttct gttcttcatg aagcaggcgt gtggtattgt ccaggaaaag
    14761 ctgctaatgc tggaggagta gcagtatctg ctttagaaat gagtcagaac gtccagcgat
    14821 tgacttggga aagagagcaa gtggatgagc agctggatca aatcatggaa acgatttacc
    14881 atacgtgtcg ccaaacagca gccgagtacg gcgacgaaaa aaatctgctt cttggagcaa
    14941 atgttgctgg atttgaaaaa gttgccaaag caatggcagc agaaggttta gtttgaaaaa
    15001 tgaaactttc ttggaaatat tgtcagcatt ttgtttattt gcttgaaaaa taataaaaaa
    15061 aaacgtaaag tattacctgt agagtaaacc tataaggagg agaacaaatg gcacacattc
    15121 attttgacta ttcaaaagta gcaccgtttg tcaacgaaca agagttaggg tacatgcaaa
    15181 gccaagtaac agcagcccac aacgaattac gggaaggtac tggcgcagga agcgacttcc
    15241 gcggctggat cgatttacca acgaactatg acaaagaaga atttgcacgt atcaaagaag
    15301 cagctaaaaa aatccaatct gattctgaag tattagttgt tatcggtatc ggtggttctt
    15361 atttaggcgc tcgtgcagcg atcgacttct tgaatcacac attcttcaat ttattagata
    15421 gcaacacaag aaaagcacca caaatcttct ttgccggaaa ttcaatcagc tcaacatata
    15481 ttgctgactt gatcgaagta atcggtgatc gcgacttttc agtaaacgtt atttcaaaat
    15541 ctggaacaac tactgaacca gcgattgcgt tccgtgtatt caaagagctt cttgttaaga
    15601 aatatggaca agaagaagca aacaaacgta tctacgctac aacagataaa gcaaaaggtg
    15661 cagttaaagt agaagcagat gcagaaggtt gggaaacttt cgtgattcct gatgacatcg
    15721 gcggacgttt ctctgtatta actgctgttg gtttgttacc aatcgctgtt agtggtgcag
    15781 atatcgatgc attgatgcaa ggtgccgctg atgcaagcaa agcatactca agcgacaaac
    15841 tagaagaaaa cgaagcttac caatacgcag caatgcgcaa cattctttac cgtaaaggca
    15901 aagtaactga gttgttgatc aactatgaac caggaatgca atacttctca gaatggtgga
    15961 aacaattgta tggtgaatct gaaggaaaag accaaaaagg catctatcct tcaagcgcaa
    16021 acttctcaac tgatttgcac tctttaggac aattcatcca agaaggacgt cgcaatattt
    16081 ttgaaacagt tgtgaaggtt gaaaaacctc gtaaatcaat cacaattcct gaacaagcag
    16141 aagatttaga cggtctaggc tacttgcaag gaaaagaagt ggacttcgta aatacaaaag
    16201 catttgaagg aacattgctt gctcatacag acggtgacgt gcctaacttg ctagtcaaaa
    16261 ttccagaaat ggatgcttat acattgggtt atacaatgta cttctttgaa attgctgtag
    16321 gtatttcagg ttacttgaat ggaatcaacc cattcgacca accaggtgta gaagcttaca
    16381 agaaaaacat gttcgcactt ctaggcaaac caggctttga agatcttgct aaagaattga
    16441 acgaacgcct gtaagataag ggtttttcgc tagatttaaa taatcagtga tagaaacatg
    16501 ttcgctttaa aatgaaaact ggtacccatt taggtactca aacatactgc atcgtttatt
    16561 cagaataagg ggctgtgaca agacgtttgt cacagtccct tgtttgtata gaaatcgatt
    16621 gattgccttg ctctttagtt tgttttagga ctttaagaac agtgaggctg acttcgaagg
    16681 ttaattctgt ttttttgttt tctagaatca gtaagcaaaa aagcactcac ttcgagtgct
    16741 tttttgcgat atattcttag cttaagatag gcctaatttt tcttttacag atgagggtac
    16801 cttatcgctg gtaattactt ttttctctat gatccgcttt tcgcttgctt tcaactgcaa
    16861 gatcgttcct tcggggtaag cagaaccatt ggcagagtca ccagaagtaa tgacatcgaa
    16921 atctaactgt cttttttcac cgttttcagc ataagcatct acgttgtatt taaaacctct
    16981 tccaattact tccccattat ctgctttgat atcagtttct gcaggcattg gagctttgac
    17041 taccgcataa taatctttac ctacataagt agactgatag tagtcccaac ctttccatcc
    17101 tataaaagca atcaatatga taacgatcaa ccctaaaatt tttttcatga aagatctcct
    17161 tttttatttt tattataccc gtggtgctag ttaattttaa tatcctaaag aatatctcaa
    17221 agttccttca aaacgtcgtt gtgctcgact taatagaaca ctgtaagcca gtaatatgct
    17281 ttcaaatcta ctctctagcc ctttaacaga acgtgagttg aaattttgac aacctaattt
    17341 ttctaaggaa gaaaaaacag tctcaactg
//
LOCUS       DARBDV010000064        17113 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000064 DARBDV010000000
VERSION     DARBDV010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.064"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            101..1438
                     /locus_tag="SCM56_001982"
     CDS             101..1438
                     /locus_tag="SCM56_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HEG1110741.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1486..3615
                     /locus_tag="SCM56_001983"
     CDS             1486..3615
                     /locus_tag="SCM56_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEG1110742.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3590..4279
                     /locus_tag="SCM56_001984"
     CDS             3590..4279
                     /locus_tag="SCM56_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110743.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(4687..5373)
                     /locus_tag="SCM56_001985"
                     /pseudo
     CDS             complement(4687..5373)
                     /locus_tag="SCM56_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5509..5703)
                     /locus_tag="SCM56_001986"
     CDS             complement(5509..5703)
                     /locus_tag="SCM56_001986"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110744.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5753..6193)
                     /locus_tag="SCM56_001987"
     CDS             complement(5753..6193)
                     /locus_tag="SCM56_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEG1110745.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(6197..7042)
                     /locus_tag="SCM56_001988"
     CDS             complement(6197..7042)
                     /locus_tag="SCM56_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEG1110746.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            7191..8609
                     /gene="pepV"
                     /locus_tag="SCM56_001989"
     CDS             7191..8609
                     /gene="pepV"
                     /locus_tag="SCM56_001989"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEG1110747.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8666..9613)
                     /locus_tag="SCM56_001990"
     CDS             complement(8666..9613)
                     /locus_tag="SCM56_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEG1110748.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9836..10186)
                     /locus_tag="SCM56_001991"
     CDS             complement(9836..10186)
                     /locus_tag="SCM56_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEG1110749.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            10386..11465
                     /gene="pepA"
                     /locus_tag="SCM56_001992"
     CDS             10386..11465
                     /gene="pepA"
                     /locus_tag="SCM56_001992"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEG1110750.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            11609..11929
                     /locus_tag="SCM56_001993"
     CDS             11609..11929
                     /locus_tag="SCM56_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEG1110751.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            11951..12415
                     /locus_tag="SCM56_001994"
     CDS             11951..12415
                     /locus_tag="SCM56_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEG1110752.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            12620..13225
                     /locus_tag="SCM56_001995"
     CDS             12620..13225
                     /locus_tag="SCM56_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HEG1110753.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(13264..14553)
                     /locus_tag="SCM56_001996"
     CDS             complement(13264..14553)
                     /locus_tag="SCM56_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEG1110754.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            14981..15460
                     /gene="rlmH"
                     /locus_tag="SCM56_001997"
     CDS             14981..15460
                     /gene="rlmH"
                     /locus_tag="SCM56_001997"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEG1110755.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            15663..16213
                     /locus_tag="SCM56_001998"
                     /pseudo
     CDS             15663..16213
                     /locus_tag="SCM56_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299296.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
     gene            16284..>17113
                     /locus_tag="SCM56_001999"
     CDS             16284..>17113
                     /locus_tag="SCM56_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HEG1110756.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT         5444 a         3102 c         3463 g         5104 t
ORIGIN      
        1 tggtcaccct ttaatgaaag cgctaactac ataagttaga atgagtttga ctgatatatt
       61 agtttgctta tcagaaaaac aaaatggacg gaggaataac atgagtgata ttattattgg
      121 tattttgtta gttatctcat tctttttcat ggtctggtac tgtgtaaaag gctacaactt
      181 gatggtcggc tttgccatca tggctactgt atggatggga ttagcgctag tcggaaacac
      241 gttttcacca aacccggcga tggaagggca aggagtaatt gatgtgttga cgcatatcta
      301 tacgacgggg ccagctgagt acgccaaatc gatcttggta aatgtcttct ttggtgcctt
      361 tttcgggcgt gttctagtag atagcggaat cgcagctacc ctgattcgaa aggtagttga
      421 acttggaggg gacaagccaa gaatcacgat gagcttacta tgtattgtaa ccgctgttat
      481 attcatgtcg atgacaggaa tcggaccagt gatttctatt gcagttattg tgttgccaat
      541 cttgatgtca ttaggtattt cagtaccggt tgcactgttc tcatttatgg gctcgattat
      601 ggctggaatc tttgctaata tcgtcaattt caaacaatat caaacgattt atgctggttt
      661 caatccagcg gctgaaagct atacttataa tgactatttc caaattggta tgattgggat
      721 gattgtaagc ttagttgtgg tactaacggt cgctaatata tcaatgaaca aaaagaaacg
      781 ctatgcaatg gcagcaaacg taccagctga aggtggcgac gcaccaatga tctcatggtt
      841 agccgtatta ttaccagttt taggggtcgt tcttttagat cttccaatca tcttaggttt
      901 tattctagca ggtatctggg cattgctgtt tactggaaaa ttgcgcggag gttataaaga
      961 aatctgtcgt cagtttgcga aattatttac agatggcgca gtagatgttg cgccaatggt
     1021 tggtttcttg atgaccttag cgatgttcaa taattcagct gcgtatgctt ctccttattt
     1081 ttcagctatt tttggagatt ggataccgca atcaccatta gtcttggcaa tcgtatttgc
     1141 tatattgaca ccactaggat ttttccgcgg tccaatgaat ctagtcggtt caggctcagc
     1201 tattttagct gtcgtattag ctgttaatcc aacgatgtcg ccagcctttt tgttcccgtt
     1261 atttgcaatc acaacgattg ctcctcagca tttagatatt acacaatctt gggttgcttg
     1321 gggattaggt tatacaaaag taacttctcg agaatatatg aagaaatcca tcccaactgg
     1381 ttggatcatc ggagcgatct tatgcctgat cacgttctta ttgtacggga acgcctagta
     1441 aacaaaacgt cgataaaaac gattagatga atggaggaaa agaaaatgaa acgttttgtg
     1501 cgaatgggaa ttgatgttgg cggtacacat acgaaagcag tcgcaatcga taatgcgacg
     1561 cacgaaatca tcggaaaatc ttcagtgaaa acaacccacg atgatgtccg tggagtagcc
     1621 gcaggtgtcg ttcagtcttt tcaaaattgt ttgctggaaa ataatatcag tccagaagat
     1681 gttgtgtttg ttgcacattc taccacacaa gcgactaatg cactgattga aggagacgta
     1741 gcaaaagtcg gcgtaatcgg gatggcaaaa ggtggattag aaggtttttt agcaaaacga
     1801 caaactagat tgaacgatat cgatttaggg aataagaaaa aaatagagat tgtcaatgca
     1861 ttcttacctg tcaaacactt aaatgtagac cgtgtttcag aaacgatttc ttctttagaa
     1921 agagaaagag cggaggtctt ggtttcttct atggcatttg gagtagataa tggggaacca
     1981 gaacgagttg tttatgaagc agcatcggta aaatcgatcc ctacaacgat ggcttctgac
     2041 atcacaaaat tatatggatt gacaagaaga actcggacag cagcaatcaa tgcatctatt
     2101 ttaccaaaaa tgctagatac ggcaacctct acagaagatt ccgtacgaga agcaggggtc
     2161 aatgtttctt tgatgattat gcgcggagac ggcggagtaa tggaaatcaa tgaaatgaaa
     2221 aaacgacctg ttttaaccat gctttccggt ccagcagcat cagtcatggg ctctttgatg
     2281 tacttgagag cgtctaatgg tgtttatttt gaagtaggcg gaacaacaac caatatcggg
     2341 gtcattaaaa atggacgtcc agcaatcgat tattcaattg taggtggcca cccaacatat
     2401 atctcttcat tagatgttcg ggttctaggt gtggcaggcg gttcgatggt acgggctaat
     2461 cagtcaggaa tcatcgatgt cggtccacgt tctgcacata ttgccggtct agattatgca
     2521 gtatttacgg agacagaaaa aatcaaaggt cctaaggttg aatttttctc tcctaaagaa
     2581 ggagaccctg ctgattacgt caaggtagtg atggaagacg gagaagaagt gactattaca
     2641 aatacatgtg cagccaatgt ccttggatta gtgcaagaag aacatttttc ttatggaaat
     2701 gtcccatctg cacgaaaagc catccaagca ttagctgatt attgtcatac gacggttgaa
     2761 gacatcgcag aacagatcat ggaaaaatcc tatgctaaga ttgaaccggt gattttagaa
     2821 ttggcagaca agtatcactt ggagaaagat cagatttctc ttgtaggtgt agggggcggc
     2881 gctgcctcat tgattactta ctttagtaat aaaatgggtg tgaaatattc gattcctgaa
     2941 aacgccgaag tgatttcttc tattggtgta gcacttgcaa tggtccgaga tgttgtagaa
     3001 cgtatcatcc cttcgccaag taaagaagat atacgatcgt tgaaaaatga agcgatgaat
     3061 aaagcgattg aatcaggcgc tacaccagaa tcgatcgaag tccacgtaga aatcgatcca
     3121 cagacttcaa aagtaacggc tatcgctact ggttctacgg aagtgaaagc aaccgatcta
     3181 accaaggaga tcacaacaga agaagcacta gagcttgctg cagaggatat gcgattaaat
     3241 aaaaatgaag tttgcttatt ggaaaataca cctttctttt atgtatgcgg cgaacagaat
     3301 cgttcaaaaa atgctggaag tcttcggatc atcgatcaaa aaggatttat caaggtccaa
     3361 cgtggacatg cttcatgtat gaaaacaact gcagccaatt atatgacagc tgtcgaacaa
     3421 ctttgggaag atatggcagt ttatcaaact gaattgattg cccgaccaga attttatctc
     3481 tgcttaggag caagagtctc tgattttaca gcgacggatc tggaacaact acagcttttg
     3541 atggatctgg aagtatctac tatggaaccg gaggaagaag tgatcgtcgt tgccggaaat
     3601 atcaaacaaa cataagttag aagagttact aaacgaatta caacagatcc ccgaagaaat
     3661 ttggggattc taccagtttc ggcgagattt attttggaaa aagattcctt tgtccaagca
     3721 gaaaattctt atacaacaat caatcgattg tggtatcgaa acggcatgta gcatcaaaaa
     3781 aaagtaccct tttgccgatg taggagaaat ttgtgaacaa atggctgttc caatcgtttc
     3841 ctgtgaatca gagcaaataa acgaacgaat cacttttgca acttatgcag aagatgaagg
     3901 gattcgattg atgacggagc cgttagaaaa attaaaatgt tcaggactag cttcgatctc
     3961 taaagaaaca gcgcaagctt tgattattgg acatgaacta tttcaccaca tcgaagcttc
     4021 tgttaaggga atttatacgc aaagcgaaaa aattgtgctg tggcggttgc ctttttatac
     4081 acatcaatca aacattcgtg cattgagcga gatagcagcg atgagttttt caaaagagat
     4141 gaatcaaagc gatttttcgc cttatgtatt agaggcagtc ttactttggc catataatga
     4201 aattcgtagc caagggatct tagaagaaat aaaaaaaata gaaaaaagat gtacggagta
     4261 tgatttagat tacaaataaa gagactgtga caaaagtcgt gtcacagtcc tttttttccg
     4321 aataaacggt gttctcctgt caactcctcg gtcacaagtc aaaatattca acaaacatga
     4381 gaagctgttt tttgaaattg ttccttgtga ctcggagatg aacggcaggc tcacgacctc
     4441 ttggaaaacg gtgttcaaaa gcttgcatct gcggtaataa gtccagccaa gttaaaaaca
     4501 taagaagcta tttttacttg gctgttctta ttacttgatg cagggcagct ttttcacaac
     4561 ctctcaataa acggtgtgca atcagctgac cctcggtatt agctcaaaaa aaacggcaaa
     4621 atatgaggaa ctattttgcc gtttctttct ctaatactca ggtcaaaacg ctgattccaa
     4681 aacctcttat ttgatgggtc tgtcctgaat atcttcaaaa taatcggggt aattatgaat
     4741 cacgtattct tgctcctcga taatcatatg taaatgcatc gtgcaaagaa atccgacttc
     4801 atcaaagtta tgatcaatca ttgattgaag cattgtctga tgttcatcta atactcgacc
     4861 ccagtctaat tctgaaatcg tcaaacgtaa ccatctgaaa cggttcaagt gcgtactata
     4921 actttctagc caatcccaaa tttctcgttt tccagcaatt tcataacaag ttcggtgaaa
     4981 atcagtatcc aaatcaaaaa aagctttttt attttgagcg attactgcct gatcggtttg
     5041 ttctagtatt ttttctagtt tagcttgatc ttacttggtc attttcgcac tgcattcttg
     5101 caggatcggt ttttccaact tttcccgcgt ataacgggct aatgaagcag atcggatatc
     5161 gatttttgaa acataagtgc cggactgtgg aatggttttt acaagctctt gattgcgcag
     5221 ttggatcagt gcttctcgaa tcggtgtacg cccgatagct agttgttctt ctaacacttt
     5281 atcggagatt ttacttccag gtgctaactc ggaataaatg atttgtttac gtatagattc
     5341 ataagcttgc tgttgcaaat tattgataac catttaacct tcctttctat gcagatataa
     5401 tctattataa cataaatcgc ttacaaatcg tgtgggcata aaaacacagt ctgcaaaaaa
     5461 agccctgata ggcatgccta ttagagcctc gaaaagaaaa atattgaatc aatctggtcg
     5521 tacctcttgg atcggtttga cacgaccgcc gattctttta gcaaaagctt cggattcttt
     5581 tttgtctttg aaaatgagca gattgtctaa gtttttttcc cgagtgcgac agccagtttt
     5641 atcgatgtaa ccgtgcatta ctttaacgac atacatgggg atcacctgct tttttctttt
     5701 cattatacga actactcgac acaatacaag aaaaatgctt agacggtctt tattaagaat
     5761 ttacatcatc caaagaagcg tacgcttgga ataactgatc taactccgga ttgactttgc
     5821 tcagcttcac taatcgttgc gaatcagaaa gtaaaaaaca gtgtttacta ctgccagtag
     5881 tttttaattt tttgtcttca gttcccacaa tttcgtagct aaaatccaac cgtgtccccg
     5941 tatacttttg gatagttgga tggatgctca ctttttctcc gtaatggatc atttctttat
     6001 agctgcaatt tacttccaga acggggatga tgatcccaga ttcttcgatt tttttatagg
     6061 gaaaccctag atgtaccaat aaggctaccc gagcctcttc aaaccagcgg atataattgc
     6121 tgtgatgaat aatccccatt tgatctgttt cataataaaa aggttctcgc acataacctg
     6181 taaatttctc catgactcat gcctcgtttt ccattgattt tttacaaaaa taattcatcc
     6241 atttcggaag tgcttcgctg atttgtgtcg gcaaaacaat atattgttct tttgccagct
     6301 ggtctccaat gaaactatga agataaatag ctgctgcaat cgtttggtca ttttgaggaa
     6361 actgggctaa aaatcctgtg atcatcccag ctaaagtgtc acccatgccg cccgttgcca
     6421 tagcagcaga acctaaagga ttggtgtaga agtgctggtg accataaatt tttgtctcat
     6481 gacttttcaa aacgagcaca gcaccgattt cctgttgctt ctttttgttt tctttatccc
     6541 gttgttcttg tatcggtaga gaactcaacc gttgccactc catttgatga ggtgtaagta
     6601 cagtttgttc gggatagggt agcaaaagat gattagcagc aagtaaactt aaagcagagc
     6661 catcaatcac cagccattgt tcttttgatt ggttttctag tacttgtttg aaacgttcca
     6721 aactcttaga agaagtaccc aatccgggac caatcaagag tacatctgct tgctttatta
     6781 cggaaagaaa ctggtcagta tccgtccaaa ctacaatcat tgcttctggc agatgactgt
     6841 gaagagcact ccgatttttt tcatcagtta ctacagtcgt gagacccgct ccgctgtgta
     6901 cggcagctaa agcactcata atgattgctc cgccatattg ttcattgccg ccaatcaaaa
     6961 cgatacgtcc aaatgttcct ttatgtgaaa tttttggacg aggtgtgatg actcttttca
     7021 ctagttgttc gtttatcagc atctaaaaac ctccttttac tcattatagc gtatattttt
     7081 tttagagaaa aacagataag aaaaggaatt gtcagatttt ttgaactatt tttttctgta
     7141 atttgtggta tcatgaacac gaagacttaa ataagtggag gattaacttt atgacaattg
     7201 attggcaaaa agaagtagaa gcacgtaaag aagacttgct tgaagacttg aaaaatcttt
     7261 tacgtgtcaa cagcgagcgc gatgactcaa aagtaacacc agatgcacca tttggacctg
     7321 gacctcgcga cgcattgaaa catatgttag cttatggcga acgtgatgga tttaaagtaa
     7381 aaaatgtcga taactatgca gggcatattg atcttggcga aggagacgaa acgctaggga
     7441 tatttggaca catggatgtc gttcctgcag gagatggctg ggacacagat ccttatgaac
     7501 cagtaatcaa ggatggaaag atctttgcac gcggatcaag cgatgacaaa ggtccaagta
     7561 tggcagctta ctatgcgatg aagatcatta aagaacttga cttgaaacta tcaaaaaaag
     7621 ttcgcttcgt agtcggcagc gatgaagaaa gcggctgggg cgatatggct tattatttcg
     7681 aacatgaaga agaaccagat tttggtttct caccagatgc tgaattccca atcatcaatg
     7741 gtgaaaaagg aaatgtttct ttagctttac gcttcaaagg cgacaatgca ggagattatg
     7801 tcttgaaatc ttttgtttcc ggacttcgcg aaaatatggt gccaggaaca gcaactgcag
     7861 cacttcaagt accaagtgca gatgcagcta tcgcgatgga agaagcattt taccaattca
     7921 tcgaagcaaa tccagtaagc ggaacgattg aagcggacaa tacgtatgta aaaatcgaat
     7981 tagttggtaa aggcgcacat ggtgcaagcc cacaatctgg tatcaatgca ggttctttct
     8041 tggcattgtt cttagataac tatgaattcc taggatctgc taaacaattt atccatgtag
     8101 cagctgctta tgtccatgaa gatttttatg gagaaaaact aggagtcgct tatgaagatg
     8161 aaaaaatggg caaattgaca atgaatgctg gactatttgc ttttgaagaa aatggtacag
     8221 aggaagcgaa cttcatcaat atgaatttcc gttttccaaa aggtgtaaca gtagacggat
     8281 tgcaaagtga catcgaacaa acagtggggc aagaaggcgc aacagttact cgaggtgctc
     8341 gcgtaatgga accacattac gtaccaatgg atgatccatt ggtggctaca ttattgcaag
     8401 tctatgaaga ccacactgga gaaaaaggct acgaacaaat catcggtggc ggaacttacg
     8461 gacgtttgtt gaaacgtggc gttgcttatg gagcgatgtt cccaggatat actgatacaa
     8521 tgcatcaagc aaacgaattt atgagtcttg atgacttgtt cagagcaaca gcaatctatg
     8581 cagatgccat ctatcgtttg gcgaaataat taattaaaaa gaggatcaag aattgcaaat
     8641 tatttttgca gtcttgatcc tttttttact ttctactgtg gtattcatga taaacatgat
     8701 atagattcaa ttcatattct ttcttagcgt tcgtgacgac tgtatccaaa atcaaccctg
     8761 ttactaatga aagaagtgct aaagtcatca atccagtact taaaatcgca gaagggatgc
     8821 gtgtaataaa gcttgtcagc atatattcgc gaatcacagg aatccccgtg accaatccaa
     8881 ataaaaagaa gaagatggtc caaatcccaa agaaaagcaa aggcttataa tctttaaaca
     8941 ttttaataat catcatgatc actttgaatc cgtcagagaa tgtattcaat tttgattcgc
     9001 tgccttcagg acgatcacga taatcgattg ggatttcaac gaacttgaat tttttatcta
     9061 aagcatggat cgtcaattcc gtttcgattt gaaaaccaga actcataata ggaaaacttt
     9121 tgacaaagat acgattgaat cctcgatagc cggtcatgac atcaagaatt tttgttttat
     9181 acattctagt aattgtattt ctaacaaggt tatttccaaa atcgtggaaa ggtctcttat
     9241 tctcatcaaa gtaggttcca tttgataggc gatcaccaat gaccatatct gcttcaccag
     9301 aacgtaattt ctcgagcaaa ccatgtacag cttctgcagg ataagtatca tcgccatcta
     9361 ccattaaata atagtcggca tcgatatcaa agaacatttg gcggatcaca tttccttttc
     9421 cttgacgagg ttcttttttt acgattgctc caccttgaac ggccaattcg tatgttcgat
     9481 cggtcgaatt attatcatag acataaatat ctgcttctgg taattctcgt ttaaagtctg
     9541 caatgactgt tgaaattgtt gcttcctcat tgtaacaagg aatcagaaca gcaatttttt
     9601 cttgttgttc catttttcac tctccttaac tatagaaaac aaaagctaaa ttaaaaatac
     9661 ctcataatca aaggaaatgc tagtttttcc cgctaaatat catttattta aaaagaaaat
     9721 actggaatat atgtttggaa agctctatct aaaaaaccac ccactacaaa agaaggacat
     9781 gatacctctc ttttgaatag tgggtgcgta ttcgttctct cacttttttg acgtattaat
     9841 ctgttgcacc tttttttctt gaaatttcta atacggttcc tgtatgagca tctgctaaaa
     9901 attcataata aacagtttcg ccatcttcta ttcgagaaag acctccccga taagcactcg
     9961 accgaacggc gaatttccta ataggttcct tttcaaaaga gatccaagaa ccttcgattg
    10021 caccttcttt tagaaaagat gacttgatat tttcaagtac ttggtctgct gacaatactt
    10081 ttttgcgatg ataccaaatc gttgaagcta ccccgctcaa taatcctaga ccgattccaa
    10141 ctgctaaccc gcctttaaaa tagcttagtt cgctttcttc atacatgtgg acatacctcc
    10201 ttcttctcac ttgttactcc ttgtgcttat tttaccacac tctcatattt tctttgaaat
    10261 aaataaccgc taccagaaaa aataagctat aataaatacg atcggtctgc agaaaaaatc
    10321 atattttatc cgcagatcaa tataagtctg agaggagttt agtctgaaat ttagacgaat
    10381 agcatatgga agaaaaaaca tttcaacgaa tcaaagagtt aacagaatta caaggaacaa
    10441 gcggttttga acaagatgtt cgcgcttata tggaaagtca tatggaaccg ctggtagatg
    10501 agttacagtt agatggtttg ggcggcattt ttggtctgcg ccatcatgaa gaagcggatg
    10561 caccccgtgt aatggtcgca gcacatatgg atgaagtcgg attcatgctg acacaaatcc
    10621 aagaaaacgg gctttttaaa gtcgtacctc taggcggctg gaatccttac gttgtttctg
    10681 ctcagcgttt tacacttaaa acctctactg gtaagaatta tccatgtatt tcttcttccg
    10741 ttcccccaca tttattgcga ggaacaagcg gacaaaaatc agtagaagta accgatattt
    10801 tgttcgatgc tgggtttgaa tcaagagaag aggcaatgag ttacggtgtc cttccaggag
    10861 acacaatcgt tccttacgct gaaacgatca aaacagccaa cggaaagaat attatcagta
    10921 aatcatggga caaccgctat ggctgtacaa tggtattaga agctttagaa gcgttgcaaa
    10981 atgaaacatt aggtcataca ttgattgcag gcgcgaatgt ccaggaagaa gtaggcttgc
    11041 gtggttctaa agcttctgtc aataaattca agccagatct gttctttgca gtagactgct
    11101 ctgcagcaga tgatacagtg acgaaaaatg gcacattcgg acatttagga gaaggtacat
    11161 tgatgcgtat ccaagatcca ggtttgatca tgcttcctcg tttgcgcgag tacttgctgg
    11221 atatcgctga aacaaacaat atcccttatc aatactttgt ttcaaaaggc ggtacagatg
    11281 caggtgctgc tcatacacaa aacgaaggaa tcccaagtac agtgattggc gtagtgggac
    11341 gttatatcca tacgcatcaa acgatgttca gtattcgcga ttttgaagct gcacgagaaa
    11401 tgttgattca aacactgaaa ggtttggata aatcaacagt aaatacgatc gtatacggaa
    11461 aataatgcga tagtgaaact ataaaatctg gcaaaagatt gctttttgcc agattttttt
    11521 tataaaaaga ggatcaatgt ttgttagttc gaaagaaagg tgtataattt aattgaaaaa
    11581 tacataaaca gaaagtagag gacaaagaat gattatccca aatagtttag aagaattagc
    11641 aggctacgta gaaactggaa agagtgtatt tttctttact gccggctggt gcggagactg
    11701 ccgttttatc aaaccgcaaa tgcctgaaat tgaaaatgac ttttctgatt ggcaatttat
    11761 tgaagtagac cgtgacaagt acatagatgt agccgctgaa tggaacattt ttggtatccc
    11821 aagttttgtc gtgatccaag acggaaaaga attaggtcgt ctagtgaata aagatcgtaa
    11881 aacaaaacag gaaatcgaat cgttcctaga aagtgtaaca aaaggctgac atctgttagg
    11941 aggggtcaag atgaatcaag aatataagaa tattttagta ggtatcgacg gtagtgaaca
    12001 agcacttgct gctttcaaaa aagcggtaga agtggctcgc cgtaataatg ggaaagtata
    12061 tgtagcaaac gtaatcgatc agcaatttta taactttatg ggctatgcac ctattgatca
    12121 aaatctagtc gatcagcaaa ctgaaacagc caaggaaatg atcgaagaat gtaaagaata
    12181 tgggaaatct gttgattaca caaatataga aggaattatt gcatatggtt ctgttcgtga
    12241 atccatggca gtcaaattgc caaaaaaata tgagatcgat ctaatcatgg ttgggcagtc
    12301 aggtctaaat gcagtcgaac gctttatgac aggaagtgta gccagctacg tgatacgtca
    12361 ttcaccttgt gatgtcttga tcgtttctga cgaagaagaa cagccaagta attaagcaag
    12421 tggaacttgt acgacctttt taatcaatta actaaaacag taaagggact gtgacaaaag
    12481 tcttgtcaca gtcccttgtt acgaataaat ggtgttccag aagaggcttc ttcggaaata
    12541 aagcatgaaa aaaatttcat gcagtgaatg caattttttg ttaagataaa tctcagcaaa
    12601 ggataatgga ggaaagaaca tgatttttgc ttataacaaa gcacacgtcg gagacacctt
    12661 gatggttatt atagccgacg ataaaggaac agaaaacact gttgaacgca aatggaatgt
    12721 tgctcggatc aaagatgaac aaggtcaagt tgttgcttgg aactttttcc atatctccga
    12781 tcatctaaca atcgaaggga acggccaagt aagcttaaca gatgaggaat tagcgaaatt
    12841 gaatcgattg atcaaagaag caggattcga agaacagctt gaagctgatc atgaaccgaa
    12901 gatcgttgtc gggtacgtaa aaacatgcgt cccacatcct gattctgacc atttatcgat
    12961 tactgagaca gaagtagaca atggtacagt attgcaaatc gtttgcggtg cacccaatat
    13021 cgaagctgga caaaaagttg tcgttgcaaa accaggagct atgatgccag atggaatgat
    13081 gatctggcca ggaactttgc gaggggtaga tagctatgga atgatctgtt ccgctcgtga
    13141 attgcatttg ccaaatgctc cagcaaaaaa aggaatctta gtattgcctg aggatgccgt
    13201 agtcggagaa gcatttccaa aataaagagg ttgtgacaag tctttgtcac aacctctttt
    13261 ttattaattt tcactatttt gattttgttt taatgccgat tgatcgatcg tcaattcaac
    13321 agtagttgtt ttttctttcg agccttcata gtaagtgatt ttgacagaat ctcccacttt
    13381 tttcttatac aaagcagatt gaagttctac accagaagaa acttctttgt catcgatctt
    13441 agtaatgacg tcgtattgtt tcaatccagc tttttcagcc ggtgtagctg tttggacatt
    13501 tgtcacgatg acaccgttag ttacagatga aggaatcttc aagatttgtt cttgttgctg
    13561 agtagaaacg gcagataagt cgaccattgt gatacctaga gctggacgtg tcacttttcc
    13621 gtctttttcc agttgattga tgatattcac tacatcgtta cttggaatag caaatcccat
    13681 tccttctacg cttacattag aagtagattc agaagtactt gcgatcttac ttgagttgat
    13741 accgataact tgtccttcga tattaactaa aggaccacca gaatttccag ggttgattgc
    13801 tgcatctgtt tggatcgcat tgatattgac tgtttcgttc gattcattcg tacttgtcac
    13861 ttgtcggttc aaggaagaaa tgattcctga agtgacagaa tttgcatatt cagatcctag
    13921 tggggaacca atcgcaatcg caggctcacc aaccttcaga gcacttgagt caccaaaaga
    13981 ggcaactgtt tcgactttgt ctgaattgat cttcaacacc gctaaatctg aataagcatc
    14041 tgttccaact aattcagctt ttaccttcgt accgtctttc atcaagactt caagaccttg
    14101 ctggccatct actacgtgat tattcgtgac tacataagca gtattcccgt cttttttgta
    14161 gatcacgccg cttccttcac tataagcttc caaattacta tcatctgagc tgctactttc
    14221 ttcttgctgc ccgaatagtc cgccaaaacc gcttgattgg ttttgacttt gtaagttgat
    14281 aacagaaacg acagcgtctt gaactttatc taccgctttg gtgatatcag aatctacatt
    14341 gactttgaca ttttccacaa ctgtttcacc ggcagaattc tgattgccgc ttgtagcagt
    14401 agaactattg ttgccagtgc ccatcgctgc ataaaaaatg ccagctgtca gtagtcctcc
    14461 aacgatccct ccaacaagcc ccagacctaa cttacgccag aggctattat ttttactttt
    14521 tttcatcttt ggtgtcacat tttttctatc catgtgttcc tcctccaatt acattcattt
    14581 atctactgtt agtatagtct tatttgttga ctttagtcta ctttgagact gtgaactttt
    14641 tatgaaaaac tttaggaaaa atcattgttt tcagatgaaa atatttgatt ttctttttcc
    14701 acagatactg tgtaaaacta cgcggatagg attggaaaaa taagggataa aacatatcca
    14761 cagcctgtgt ataaagtgga taaattctta taaaacgtat gtttgcctaa tgaatgtgat
    14821 gaaataaggt aaaataacac tgttttctgt ggataaacct gtggatgttg tgcataactg
    14881 ttggggaaat ctttttaaca aaaatagcca aaataaacat atagacgaaa aaattgatta
    14941 taataaacaa agtgagcatg aagaccaaag gagagaaaac atgaatatca aaattatctc
    15001 agtaggaaaa ttaaaagaaa aatacctgat ccaaggtatc aatgagtatg tcaaacggct
    15061 gaatgcctat gcaaaaatcg aattgatcga agttcctgac gaaaaagccc cggagaattt
    15121 aagcgaagcg caaatgagac aagtaaaaga aaaagaaggg gaacggatct tagcgaaaat
    15181 caaagaacaa gaatacgtct atgccttagc aattgaaggg aaaaatccta caagtgaatc
    15241 ttttgctaaa caaatcgacc aattaggtat ccaaggaaaa agccaccttg tttttgttat
    15301 cggcggctct ttaggactca gcgaagccgt catgaaaaga agcaatgctc agatctcttt
    15361 tggaaaaatg acgtatccac atcaattgat gcgtctgatc cttgtcgagc aaatctaccg
    15421 tgccttccgg atcaatgcgg gggctccata ccacaaataa tgatattttt taagagaaaa
    15481 aattatccct attttaaata atagcaatag gtagtggtag ggagaaattt ataaataact
    15541 tttttatttg ctttggtagc caagtaagtt gcaaaagaaa aatagatact gttaaaaaga
    15601 taatgtagac atagaaacaa atacgtaaaa ttttagttag aataatggca aggagaaaaa
    15661 acatgataga tgaaaaaaag tgtttaaaaa atctattaga ggatatggat gatattgaag
    15721 aaaactatct aaatggactg ggaaaacaaa tatatttcag atacttaagc tatctaaaaa
    15781 tgaaataaga catagtaatt ttttagcttt tttgtttgct ccaaatgaaa cccacaattt
    15841 atctgatgag ttttttaaaa tgtttttgaa aaggtatata gattctaatg atgataccaa
    15901 agctgctatc aattattttg atgctttact taatagctat gaagatttaa ttgtttacag
    15961 agaaaataag aatattgata ttttattggt atcggaaaaa aataaaatag tggtgtgcat
    16021 cgaaaataaa attctgtctt cggagtctgg agggcaactt aataaatacc aaatatatat
    16081 agaacgcaat tattctaact ataaaaaaat ttttgttttt ttaactccag atggaaatga
    16141 acccacaaat cctagttggg gcataattac ttacaagaat atcgttgaag agagtgtaaa
    16201 atattttgtg taaatagaaa aaaggaagtc ccttctgtag aatagagtta ccacaacaca
    16261 ttcacagaaa agaggacttc catatgaacg attttactac agaaattatg gaaatactaa
    16321 tcaataaagg tgatttggat gatttgtttc gtcgtcacct agaattggct attaacgctt
    16381 tattacaagc tgaattaacg gtatttcttg actatgaaaa gtatgataga gctggattta
    16441 attcaggtaa ttcccgtaat gggaattatt cgcgctcttt taaaacagag tacggtgaat
    16501 tgaatttagt aatacctaga gataggaatg gagacttttc tcaacaaact ttacctgctt
    16561 ataaaagaac caatgactct ttagaaacca ctattattca gctagttcaa aaaggaatta
    16621 ctatgtccga aatctctgat ctaatcgaaa aaatgtatgg tcattattac aaaccacaaa
    16681 ctatttcaaa tatgagtaaa attgtgtctg aagatgtttc ggcatttaaa gaaagaactt
    16741 tagaagctaa atactcagtc atttttatgg atgctactca tattccctta aaaagacaaa
    16801 ccgtatcaaa agaagctgtc tatatagtaa ttggtatcag gttggatgga actaaagagg
    16861 ttctaggatt tactatagct ccaactgaat ctgcttatgt ttggaaagag attcttcaag
    16921 atctaaaaga ccgtggttta gaagaggttt tgttagtcgt aacagatggt ttaagtggta
    16981 ttgacgatag tattcatagt gtttatccaa atgctcaatt tcaacaatgt tgtgtccata
    17041 tatctagaaa tatcgctcat aaggttcgtg ttagtgatcg acaagaaatc tgtagcgatt
    17101 tcaaattagt tta
//
LOCUS       DARBDV010000065        16671 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000065 DARBDV010000000
VERSION     DARBDV010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.065"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(160..867)
                     /locus_tag="SCM56_002000"
     CDS             complement(160..867)
                     /locus_tag="SCM56_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEG1110757.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(946..1284)
                     /locus_tag="SCM56_002001"
     CDS             complement(946..1284)
                     /locus_tag="SCM56_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEG1110758.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(1395..2357)
                     /locus_tag="SCM56_002002"
     CDS             complement(1395..2357)
                     /locus_tag="SCM56_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEG1110759.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(2372..3703)
                     /locus_tag="SCM56_002003"
     CDS             complement(2372..3703)
                     /locus_tag="SCM56_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEG1110760.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(3711..4391)
                     /locus_tag="SCM56_002004"
     CDS             complement(3711..4391)
                     /locus_tag="SCM56_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEG1110761.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(4610..5089)
                     /locus_tag="SCM56_002005"
     CDS             complement(4610..5089)
                     /locus_tag="SCM56_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEG1110762.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(5216..6895)
                     /locus_tag="SCM56_002006"
     CDS             complement(5216..6895)
                     /locus_tag="SCM56_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEG1110763.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            7443..8945
                     /locus_tag="SCM56_002007"
     CDS             7443..8945
                     /locus_tag="SCM56_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1110764.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            8958..9620
                     /locus_tag="SCM56_002008"
     CDS             8958..9620
                     /locus_tag="SCM56_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1110765.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(9721..9924)
                     /locus_tag="SCM56_002009"
     CDS             complement(9721..9924)
                     /locus_tag="SCM56_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEG1110766.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(10035..11219)
                     /gene="iadA"
                     /locus_tag="SCM56_002010"
     CDS             complement(10035..11219)
                     /gene="iadA"
                     /locus_tag="SCM56_002010"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HEG1110767.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(11376..13115)
                     /gene="yfcC"
                     /locus_tag="SCM56_002011"
     CDS             complement(11376..13115)
                     /gene="yfcC"
                     /locus_tag="SCM56_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HEG1110768.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            complement(13888..14502)
                     /locus_tag="SCM56_002012"
     CDS             complement(13888..14502)
                     /locus_tag="SCM56_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEG1110769.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(15108..15485)
                     /locus_tag="SCM56_002013"
     CDS             complement(15108..15485)
                     /locus_tag="SCM56_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110770.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(15785..16666)
                     /gene="galU"
                     /locus_tag="SCM56_002014"
     CDS             complement(15785..16666)
                     /gene="galU"
                     /locus_tag="SCM56_002014"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEG1110771.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT         4831 a         3593 c         2847 g         5400 t
ORIGIN      
        1 aatctgagcc agaatgaggt tcattctgac ccagatttga ttttgtacaa cccatcagtc
       61 cttattgaca aagagccctt attccaaata aatggtattc aatcagctaa ctctcggtat
      121 tagctcagat caaagcgctg atttcacaac ttctttattt taatctcttc ggttgccgcc
      181 gaacaataag atcaagcgca acagctgcag gaatgtcgaa agcgctgctg caacataagt
      241 cagtgctgct gcaaataata cgtgtcttgc catcggtact tcttcttccg tcagtagtcc
      301 gccttcactc aaaatttgga ttgctcgccg agacgcgtta aattcgactg gtaatgtcac
      361 caattggaaa agtaatgcta aagaaaaagc gaaaatcccg atattgatta acgtagagtt
      421 ccagctaagc aatacaccta ccataatcaa ggggaacgaa atcatcgaac caaagttagc
      481 tgctggaaca agtgctgcac gaatcttcaa tgggacataa cccgaagcat cttgtaccgc
      541 atgtccgcat tcatgagctg ctacacctat cgcagcaact gaagtagact gtgcagttgc
      601 ttgagacaaa ctcaacatct tattacctga attgtagtta tctgtgagat ctccggcaat
      661 ttgttgaaca ccgacattat ttatctgttg actttgtaat atatattgtg ctgcttgtgt
      721 accagtcaca ttgtttttgc ttctcacttg atcgtatttt cggaaagtac tattgacgta
      781 cgctgacgca attccggaaa taactaatcc aagaatgacc aaaagaaatg tcggatctaa
      841 aaaaccatac attccataac catacatttt cgtttcactc cttttcgttt actttatttt
      901 atcattagta gtaggaaaag aatgtgaaaa gaccttgaat attctttaat tctttttcac
      961 aaattccgat ttcagtttca tcggtccaaa tccttctacc ttgcaatcga tattatgatc
     1021 gccttctact aaacggatgt tcttgacttt tgttccttgt ttaatggcac ctgacgcacc
     1081 ttttactttc aagtctttaa tcactgtcac gctatcgccc tctaacagac ggtttccatt
     1141 tgcatctaat accactaatc cgtcttctat gacagtttct tgttcttctt tgttccattc
     1201 gttcccgcat tctgggcaaa tcaacaagcc tcgatcttca tatgtgtact ctgagccaca
     1261 ttctgggcaa tttggtaatg tcatcttctt tctcctttaa aatctttagt gagcactatt
     1321 gtaacggaaa aagaagatct tgaaaagata catttgtgat ttttactaca aaaggcacgt
     1381 tttcgaacga agtatcattc tgtttttctg ctcttatacg attggcatat atcttggatc
     1441 tctttataga tttcctctac ttctgctaaa tcttttgcat tcgcaccact tgctaatgga
     1501 tggcctcctc catgatggcg tttagccaat tcattgatga tcggcccctt agaacgtaaa
     1561 cgtacgcggt aataccctga aggctgctga acaaaaatcc cccaagccag cacttcgtca
     1621 atcactccag gaagtgaaac gatcgaagct gtctctgaat catcgattcc atatttatca
     1681 agaatttctt gcggaaggat tattcttcct gcaccatttt catccacctg gatattttga
     1741 taaagataac cggaaagttt agcgactttc atcggcattt gttcaatctc tctgttcaaa
     1801 gcacttgcat caaatggata atttctcaat atcgaagcaa tctctaatgt acgggacgtc
     1861 gtggagggat acaaaaagcg ccctgtatct ccgacgattc ctgcataaag taaccgtgca
     1921 gccttaacgt tcatctttaa agattcagga aacatcaaag caaattctgc aatcatctca
     1981 ctacagctac ttgcttttgt atttacccac acaagatctc cataagggtc atcattggga
     2041 tgatggtcga ttttgatcag tttttctgcc aactgatatc tttgatcact gattctgggt
     2101 gaattagctg tatcagtcac gatgactagc gctccttggt agaagacatc ttcaacttct
     2161 tgcatttctg ctaaaaagcg aagcccttca atcgtttcac ctacttgacg tatttctttt
     2221 tctgggaaac tttcccgtaa tagttcggct aatcctactt gtgatcctaa tgcatcagga
     2281 tcagggcgca aatgcctgtg gataatgatt ttatcgtatt tttttattgt atctactatt
     2341 tcttgctgta ctgtcatttc actcctgctt tctaagaacg ttccattact tgacaaacaa
     2401 cgattgcttt ggaaacgatg gtgttttcaa taaacacttc gatatccagt ttagcggaac
     2461 gtctgccgat ttccaataca cgtggacgaa tgtctaattc gctttccaat tggatcagcc
     2521 gcaaataatg taggttcact tgttcaatca agatatttcg tttttggttc atcaccattg
     2581 ttttttgtgc gacatcagaa atgatttcgc taagtacgcc aaaagaaatg gttccaacgc
     2641 cattgaccat ttgtggtgct actacgaatt tgaacttagg gttttccaaa cgatttcctt
     2701 cacggtcagt atcaatcgta tggatatttc cagatatctg atccgaaatc gtatctgcaa
     2761 tctgcggttg tcgctggaca agctgcatcg ctttcattac atcttgccgg gtaatcaagc
     2821 cttccaatgt caagtcatcg gaaacgaccg gcatgacttc tagcccatcc cagatcattt
     2881 gatggctaac agaggcaata ctcattgttt tctttacact gattggttct ttcgtcatca
     2941 cccgatcgat gatctggttt ggcgatttcc ctaaaacatc ttttgccgta acaatcccca
     3001 tcaaacgatg atggcggttg acgactggaa aacgcgaatg ctgcgtttct tctgacaaac
     3061 gctgataatc agaaatcgta tctgtctgct gcaaataatg agtcttttca gctggcatat
     3121 agatatcact gacaagcatg atatcttttt tgatcaattg gtcgcttaac gctcggttga
     3181 tcatggttgc gactgtaaat gtatcataag aggtgcgtaa gatcggcatt tcaagatcat
     3241 cagctagctt tgcaatctca gcagtcgtat tgaatcctcc tgtgatcaat actgcggcgc
     3301 catcttccaa tgctaatttt tggacttccg cccggttgcc gacgatcatt agtgatcctg
     3361 gcgtaatata gcgttccatg gcatgtttag tcatcgcgcc gatgacgaat ttattcaaga
     3421 ccttgtctaa tcctgccgcc ccgcctaaaa cgtccccttc aatgatcctg acgacctcgc
     3481 caaatgtcaa acgttcaata tgttttttta atttgcgttc gatacggatg gtccctactc
     3541 gttgaattgt tgaaactagt ccgacatttt ctgcatcttt gattgcacgg tacgctgttc
     3601 cttcactgac attcaattct tttgcgatgc ttcttacaga aattcgattg ccaacaggta
     3661 aactttcaat atattctaaa atcaaatcat gttttgtcgc cattttctgc ctacacttcc
     3721 agcgtttccg agacgctcat tatttttcct tcactatctt ccagtaactc tacaaaagat
     3781 tcgggatctt gttcaatgac agggaaagta ttgtaatgga ttggaactac tttcttggca
     3841 cgaattgttt gcgcagcctt tgctgcatct tctggcccca tcgtatagtt atcgccaatc
     3901 ggcaagaaag cgacatcaat atcgaattct tctgcaagca atcctagatc actgaaataa
     3961 gcagtatccc ctgcatgata aatcgttttt ccttctgctt gaataataat tccgctaggc
     4021 tcgcccatat actgaacgat accgtctact tcatagcttg aactatgctg tgcatgtacg
     4081 aattttacca cgccgaatgg aaacgcatgt tttccaccga tattcatacc gaatgaatct
     4141 actcctctgc tgttcgcata atcagcaatc tcaacaatac tgataattgt ggcttgattt
     4201 tcttttgcaa taggaatcat atctcctatg tggtcactat gaccatgagt aatcagcaac
     4261 caatctgtct ttacttcttc tgcagttaag tctgtcaatg gatttcctgt aataaacggg
     4321 tcaatcatca cgcgttggcc attttccatt tcaattgtta aacaggaatg tccatgatac
     4381 gtaattttca tattttacac ctcgcgtttt ctctgtatct tttttattaa aaacgatttc
     4441 atcacttgca catcaatgaa aaaatatagc tgctaaatat attttcattg gattgatagt
     4501 acagtatgta caaagcaatt gtaacagata atacaggatg caccaagtac caagcagata
     4561 taatctcaag tttgaggtct ctgctctgct tagcttctct tctcgtccct taactaatta
     4621 aatcagcgta aataggatgg acgatctttt ctaaatcggc tacttttatc cgaaggcttc
     4681 tcccgatttc accgcttgac acaattactt cttctttttc ttttaaagaa ctgtctgcta
     4741 aaaaacaata atcaggatgt gcgagtttta ttccaatagg ggtgtttgct ccgtggggat
     4801 atcctgtatg cattaataca aaatccattc caggaaaacc aactttacga tctttcgtca
     4861 cttgtctcgt ttttttgtaa tctaagtgat cgttcaaagg aatcaatgcg atgatcaccc
     4921 cagttttgtt tcctttcact acgagagttt tacatatcaa agctggatca atccctcttt
     4981 tttgtagttc ttctgtgaaa gattcttcta ccacatgact aaaatcaagt ggttgatagc
     5041 tgatcttttg ttcttctaaa taacgttcga cttttgtttg actcatcatt gattcactcc
     5101 ctttattcta tcgtttatta taaagcgagg gatgctgtat tcaaaaatta tttgtttttc
     5161 gcaaaaaaag agcaagacga gcactctata gagaggttca tcttactctt tttgtttatt
     5221 ttttatttcg acgttgagta gcttccataa tgactggcaa gatcactggg cggcgttttg
     5281 tttgttcaaa taagtaacgg cctaattgat ctcggatgtc ttgctttaat ttgctccact
     5341 caaaatcatt gctttctaaa tgtttctcaa cgattgtttc aatcatattg ctgctttctt
     5401 tcatcagatc acggctagct tttacgtaaa cgaatccccg tgaagtaatt tgaggttttg
     5461 aaatgatttt cttttctcgc cgactgatag tgacaaccgc tacgaaaatc ccgtcttctg
     5521 acaagatttt tcgatcacgt aaaacaatgt ttccgatatc acctatgcct aatccatcaa
     5581 tcattacatt ttctgcactt gtagttcctg caacactcat acgcttgttc ttatattcca
     5641 gcacgtctcc gcgtccagta atatagatat ttttgtatgg catccctact gcatgagcca
     5701 aatctgcatg agcagctaat tcacgatact ctccttgaac tggcacaaaa tatgttggtt
     5761 tcatcaaatt gatcattaat tgcaagtcat taggattggc atggccagaa acgcgcatat
     5821 tctcagagat caacttcact gttccaccag ctcgataaat gatatcttcc gttttcgcca
     5881 cgatggtttc catcgcgatg cttggcgtag tagtgatata aaccaaatct ccttcttcaa
     5941 tcttgacatt acggtgtgta ccgtttgcca ttttttgcag agacttgatg ggttcaccca
     6001 ttcggccagt ttctaaaatg atcagttctt ctggatcata gtttttcatg tctttcaacg
     6061 taacaagcag atcttcactt ggtaattgaa gtttttctaa acgcatcgct gtgcgaataa
     6121 ttcgttcgaa atcttgacca gtcaagacaa cttttcgttg tgctttttct gctgcatcca
     6181 atacttgttg cactcgttgc aaattacttg ctacacaagc gacgatgata cgcccttccc
     6241 aatatttgat cgtatcaaat acttcatctg ctatttgggc ttcagaagct acaggctgag
     6301 gattttctgc atttgccgat tcacttagta aggctaagac tccttctttg ccgatttccg
     6361 ccaatcgtgc ataatctgtt tgatacattg ggatagccgt ttggtcaaat ttaaaatctc
     6421 ccgtataaac gatactacct tcttttgttt tcagattgat ccccactgaa tctggtatcg
     6481 tatgagtggt acggaaaaag ctcactactg tttcaccaaa gtctatttct gtatgctcat
     6541 ctaccacatg gaaatcctta aatcctttgg ctgcttcatt acgactgaca ttcaattttg
     6601 ctaactcgat cgtcaactct gtcccgaata ctggtacaga aatttttgac aacaagtaag
     6661 gaagtgcccc gatcgcatct gcatgtccat gtgttaaaaa gacaccagct acacgatcaa
     6721 tgttttcttc taaatacgta aaatcaggga ttacgacgtc gatccccaac aactcatttt
     6781 caggatattt tagcccacaa tctaaaataa aaatatcctc gcccacttct gcgacataca
     6841 tgttttttcc attttcacga acgccgccta atgggataat tttaatggta ctcacaaact
     6901 tcacctcttc ttatgacatc caagtttaaa gatgtcgatt ttcttcttta tctaaatata
     6961 tgagccattc caatgaagca tatgcaaaat cactatttct caacttatgg agaatcagca
     7021 ggaaaattac cagcacatcc gcgttctgac agttggatgg gcattttatt ttggatatac
     7081 ttcattaaca aaatggatct tttgcgaaac gctatagcca gaatacttat ataagtattc
     7141 agctgttccc cattattata caacaaataa actaaatcgc tagtatcagc aattgttaga
     7201 acaactatgc acgtaaaaat ccctctgtag catctaggat ttcttttgct attcctcttt
     7261 catttcttct tcgtcggttt gaaacatact tttttcattt taccacattt tttgcaataa
     7321 ctatagtgac tttttatata tacagaacct catgttgatg gtgtgcaaac agcataagat
     7381 ttaagctagc tttaagatag gttgtctatt atcaaatgca tctactagga gggatgcaaa
     7441 atatgaattt tatgaaacgt gcatggaaaa gtacaaaagt aaaatgggga cgttcattgt
     7501 tattgtttgc cgtgttcaca gccattctta tttttgtgct agctggcctc acgattcgaa
     7561 gcgctgcaga agaagcagcg aatcaagcac aaaaagaagt tggagcaaca gtgactttaa
     7621 gtgccaatcg agaagcttca tttaaaaaac aggaacaaac ttcttcaagc ggttctacca
     7681 ctgaagaacg tccagatcca ggcagttttt ctcttacacc agtttcctta acggacgcgg
     7741 aaaaaatagc tgaattagat aatgtaaaga gttattcctt tgaaagttcc gcctcagcta
     7801 tggcaaaaga tggaataact gccatcacta gttctgattc aagtgaagac agttcagaaa
     7861 ctactgatgc aggcggtgaa cctgctggag gaatgggcgg gaatccgcaa atgactcaag
     7921 cagactttca aatcagtggt gtgtcagcaa ctgcacaaaa tagtaatttt tctgatggaa
     7981 cagcaaaaat cacggatggt gaaggaatag atgacagcga caaaggcaca aacaatgtag
     8041 tcattgattc tactctagca gaaacaaatg acctggcagt cggagatact ttcacaatca
     8101 cgagtactga agatgaagat accacttatg aaatgacgat caaaggaatt tatgaatcca
     8161 gcgaaacaag cggcagtatg ggtatgaaat tcaacttcat gaatccgtcg aatacacttt
     8221 acacttctta cacttttgca aatgaactaa atgggacatc tgacgataac acgatagatt
     8281 ctgctattta cactctatct gatccagata aaatggatga gtttctcaaa gaagctgaga
     8341 agctgatcga cacagataca tttagcttac aatcaaatga ttccatgtat cagtcaatgt
     8401 tggaaccttt aaacaacgtg gcaagttttt ctaaaaatgt tgttttacta gtggctgtag
     8461 ccggtattat catacttact ctgatcatta tgattaccat tcgggagcgt cggcatgagt
     8521 taggcgtctt gctatcttta ggagaatcca gaagtaaaat cattctccaa ttgtttaccg
     8581 aagtggctat ttgtatgatt cttgcattag gagttgcttc gcttagcgga aacgtcgtcg
     8641 cgaatgccgt gggacagcag ctacttgacc aacagacaga aaccacgaca caaaaccagc
     8701 aagctcctgg cggtggacaa atgccaggta acagacaaga acaaggcggt caacgcgatg
     8761 gtcaacaagg aaccacgaat aatccatttg ctgtttctga tcaagtcagc gaattggaaa
     8821 tcaatgtcaa acctgcacaa ttgggactat tagccggagt agcatttgga atcagtctgt
     8881 tttctgtctt tttagcttct atagggatat taagattaaa cccaagaaaa atcctattaa
     8941 actaaaggag gaatttgatg ttacggacag aacaattggg attttggtat caagatccgc
     9001 aagatgcgct ttataaagat gtagaactga atttcgaagc tggaaaaatg tacgctattt
     9061 taggagcaag tggttctgga aaaacgactt ttctttcgct tatttctgga ctagatgcac
     9121 caaaagaagg aaaggtatac tatcaagatc gttcactgaa caaaatcggg ttaagaagtt
     9181 atcgtaaaaa tgatgtctcg attattttcc aagcttacaa cttgcttcct tatttatcgg
     9241 ctctagacaa cgtcatagca gcaatggaaa tcactaagac caagcaagag aattatcgac
     9301 aagttgcttt agatcgactg gctcgtgtag gaattgatga aatattagca aaaaaacagg
     9361 tgaccaaact ttcgggaggt cagcagcagc gagtcgctat cgttcgtgct atctgctgtg
     9421 aacatcaact aatcgtagcg gatgagccta ctggaaatct agatgaaaca acttcacaag
     9481 atatcgtcaa acttttccaa gaaatcgccc atgaacaaaa tcgctgtatt atcatcgtga
     9541 ctcatgagat tgaagtagca aaaagctgtg atgaagtctt tgaactaaaa aataaaaaat
     9601 ttcaaaaaat cgaagtttaa aaaacaggat aatgatcaaa taaaaaatcc gaaccatatc
     9661 tgtctcgcag atatggttcg gattttttat ttgcttgatt caattagtaa taagtggttt
     9721 ttattttttt tcgtcttttc gagaaaactt gtctttgact tcgtcgccta aatttcgagc
     9781 tttttctttg atgtcagcac ctagtgattg tgctttccct tctgtttctt tgctcttgtc
     9841 gtctgttgct ttgccgtatg cttcttttcc ttcaccgact actttatctt tcatatcttt
     9901 agaacgtcct tttaaatctg ccattgtgct tcctcctctt agtttgttta ttgatctacc
     9961 ttcagggtaa catcaacaac agatacagac aaacagaacg ctcgttttct aaaaaagata
    10021 gttagagctg ttgattaaaa acttttttcg aaagtccccc atactttgac ttctttttct
    10081 tggaccatta tttctccttt agcgataact gtatcaatat caagcgtttc tttatccaag
    10141 atacacagat ctgcatcagc accaatttcg atttttcctt ttttatccaa tttcaatata
    10201 cgtgctggat tactggttat agctcgtaag gcgatttcca aagggatact ttctttttgc
    10261 acagcttctt tgatccctac tagcaatgcc ttggcacttc ctactcctaa ccctaggaaa
    10321 tgattgtttt catcaaaata aggcaagctt ccttgtccat ctgaggtcat cgtgaaatta
    10381 tctaaagaaa catcttcctc gatcaatcgt ttcagtgctt tgctgaagcg aacttcacca
    10441 tctgtcttct cccaaaaatc aggatcttcg ctagcagtga agtcaatcgt gccaccacgt
    10501 ttggcaaatg ccacgcaagc ttcaaataat tctggtgtgc gatttgcatg agtaggcaaa
    10561 aattgtgtga tcggaatttc tgtttcttct acgattcgtg tcaatagctc tagctttctt
    10621 tttcctccgc ctaagtggac attgatgatc ccagcttttc cggataacat gcctcctaca
    10681 cgagcatctg ctactgcacg agcgaactct tcaaaacttg gctgagaaga acggtgatcg
    10741 gatactgcga tttctccgat tccgataatt ttatcaattg tcaaaaagtc tttgatgata
    10801 gaatctgtta ctgtttttac cggcaatcgg tacgaacctt cgtatacata agtggagatt
    10861 ccttctgctt ccagaccttt cgctttgctg atcaacgcag tcatatctct accttctccg
    10921 tctgttccca aacaaccgac aactgtcgtt acaccagctg tagtcaattg gctcaatgtg
    10981 acttctggcg ttcgattttg ataaccgcct tctcctcctc cacctaaaat atggaaatga
    11041 cagtcaatga aacccggggt tagtactttt ccctcacctg aaagttcttc tacttccact
    11101 ccttcgtagc cgccttctag ctgttcttct acagccaaaa tcttcccacc ggcaatcagt
    11161 acatccattt ttcctaagta atctggtgca aacacttcaa tttgtcgaat gattttcata
    11221 catttttcct ctttctttaa gtataactga tgataggatc ttaaaaaata atccaaacga
    11281 taggatcttt actcctttga ttttaccgta aaatgacggt tcttggagtc cttatctact
    11341 gtttggatta cttttttatt tacaggtctc cagtactact gcagacctat tgcaatggca
    11401 atcatgatga aaatagttcc taaaacaaac aggaatcctt gcatcttgat ttggaatctt
    11461 gcccattttg cccattcgat ccgtgcaaca cctagcacac caatcaagct ggctgatacg
    11521 ggtgtaaaag cgtccataaa acctgaacca agctgataag caagaactgc tacttgtctg
    11581 gatacaccta ctaaatctgc cagtggagcc atgattggca tagtcaaagc tgcttgtcct
    11641 gaatttgatg taacaaccaa gttgaacaaa ctttggaaga tgtacatcgc ccaagcacca
    11701 atgacagctg gaacaccggt caacgccgtc ccgatcccat gcaaaatcgt attcaaagct
    11761 gaaggaacag atgcatcaga tcctcccaag acaagaagaa tccctttggc catacctact
    11821 acgattgctg taccagctaa atcagctgca ccagattgga aagaagaagc gatgtcattg
    11881 actttcattc catctaattt gaaaagaaca gcaatgattc cagcaactaa tcccatcaca
    11941 aagaattgag aagcgatttc tggaatgtaa tagccttttt gagtcacgcc ccaaacgatc
    12001 cagacaagga caactaacat ctctaataag atcaatttat gcccaagcat gaatttttta
    12061 ttttcatcag atgtcttttt gatatgagaa cggaaataat catctgattt gtaagtcaac
    12121 gaactttctg gttttttacg gattttttct gcatatatca tcatataacc agcggctaat
    12181 gcagtaacaa ctacccacat aatcaatcgg aatgtcgcac cagacaacac ggggattcca
    12241 gcaatccctt gagccaccgc tacgctaaac gggctcatcc aagaggtcgc atttccgact
    12301 tgagatgcga cataagtcac ggttactgct acgatcgagt catatcctaa cgcaatgaca
    12361 aatggaacca tcaccattga gaatggaatt acctcttctg ccatcccgaa tgtagcgcca
    12421 ccgaatgaaa aagcaaagaa taatagcggt aaagctaaac gctccagtcc cttagtttta
    12481 ctgataaacg catagatacc tgcgtcgatc gctccggtac gcataataat accaaaagca
    12541 cctccaacaa caaggatcaa tgcagcgatc cctacagcag atccatactt atctccagat
    12601 actagtcctt caaaaacaaa atttaagaaa ccaaagccgc caaaatcatc tgttccccat
    12661 attttggctg ttttatgcaa ttttttagaa gtatcataga tattttcgcc gtactgatcg
    12721 taaagcacat catctgtcaa ggagatttca tctagttttt cctgcgtaag atttttttct
    12781 ccgtcagcaa tgactttttc caatcctttt tccggaacat ccagtttttc acgttctgct
    12841 ggatgatctt gcagttcttc taattgatca aacacatacg acttatccag ctcgtaagca
    12901 taacggaagg aatcttgacg taaaacagta cgagtacttg tttcgccatt tgcatctttg
    12961 tattcaatat cttgcgtgct gaactttcct gctggaacga caaaagtcag tatccatgca
    13021 aagatgacta caccaaaaat aatgacatac gtatggggtg tcgataattt agtgaagtcg
    13081 cgtttcggtt ttcctgaaga atttgttttc ttcaaattta tttcttcctt tcttttttca
    13141 gtaattttga tgaataaaaa atggataatc aaatgaatta tacagtaaaa atgacatttc
    13201 cacatgaata atttattaga aaatcacttt tttctatttt gttttttttc tcctatgtat
    13261 gattattttt ttctctttcc atttatatta agtattttat ttctctgttt ttccttaaaa
    13321 ttcttttttt atttttagat tagagaaaat ctcgtcctgt ttttttcatt ttacaatcat
    13381 tgaaaaattg aatttcattg caaaaaaaat aataaacact tataaatatc cattatctat
    13441 ccttcaattt ttgtgtattt tatttctatt agaaaaaggt atcctgttgg acaattttgt
    13501 tatatcaggt aaacttcatt gcaataatga taacaaatgt actataatta ctttgtaaag
    13561 agttttgggg aagactcttt catcaacgtg tattaagaga caagaaagaa tccatatgga
    13621 ttctttcttg tctctttttc cactactttt tatttttctg acactacata ttcttataac
    13681 tcatttctca taactgtgaa acaacttata aaaataacaa actttaaaca aaacaagctt
    13741 aaaatcaaag atgatccatc ttaaaataac taaaacaaaa aaagtagtcg ttgttaatcc
    13801 tcattaataa tcgttttctt tttttcaaac gctccatttt tatgtggaac attatttttt
    13861 taaggaactt tgtcaacatg tggataacta ttttccactt gtgtcctgga ctagctgttt
    13921 tgctgtaaaa tacaaaactt gctgcagcaa aaagatcgtt gcaagagagg ctgttccgca
    13981 gatcgctgga tctagatttg gcaaagtcaa gactgcatca tgtaaattgg cgtggttgtc
    14041 caaaaccaaa tcagcaaatt caaataatcg caaaccactg ctatgttgcg atttgatact
    14101 tcgtgacaaa tcaaatccag taacaatgat taatggatga ccattttctt tgatccactg
    14161 agctagttcg atgattgccg ggtttcttcc ttcattcgaa agaagaagaa aaatgtcttc
    14221 tgatttgaca gaaagctgat caataattgc ctgtcctgca ccttctagcg tttcatagac
    14281 acctccggct ggattttcaa tcaatatact tggtacaaga tccggggttt gatcccaaaa
    14341 ttcaaatgct acaccactta gtgtgcggct agcatatatc tgcaatctac caccttgatt
    14401 aatttgtttc gcgatccaag tgccagcttg aagcatcagc ggctcttcta tccttaattt
    14461 ctcttccaat tggtgataat agtcaaaata acataaactc atttatctct cccgcctagc
    14521 tatacttgac atgaatagaa acagctgcct aatctaagga agtacataaa aaaacagtag
    14581 ttagacagta acacatgtgg tctttgctgt ttcaaacaca aaaaaggcat tgaacgaata
    14641 cgcccaatac cttgacatag caaataaaac aacgtgcaga ccatggaaaa tatctacata
    14701 ggggtagaat tttcacccta gattaggtac aataaattag gtcgatgcac tgcattgact
    14761 atggcaaaat gatcggtcag ggtgtatgtg tgtgactggt cattccctga ttcttagcgc
    14821 caactaaggg tcagggagcc tttttttatt cagctgtttc tatctgaatt tattgtaagt
    14881 aatctcttat ccaagtgcaa gcaagaaaat acaaaaaaac attggtatga tttatatata
    14941 tataaataac ataaaaaaag gaatggctta tcccgataca acaagcattc ctttaaaaaa
    15001 gattctttta tataagtgaa aaaagctttt ttccaccaat taaataaatc tctcatctca
    15061 atagtttatc cattatttta tctttttttg cgtataccta ctaaatatta ccgtcttctt
    15121 ctcgttcttc tactttgttc gagagaatct gtactagaaa aagtagttgt atgtctacct
    15181 tttctgccta tccgagactt tcttttcata tatgggatat aaatgattag atacagtaca
    15241 gcaaaaaata aagcaaatac gcaagcttcc caactaccta acgccaattc aaccatcaag
    15301 gtagcaaccc caacggatgc tgcataccag ataaaaatca gaaacgtttc tttttttctg
    15361 accatgcgaa acatgataaa tagactaata agcaatagtg gcaacacaaa tatccaattg
    15421 cttccttcca tcagccactt gagtatatca agcagcgact gtgcaacagc gactaatttt
    15481 cccatagtga acacacacct ttttttataa ttaaaaaatt cgccttttta ttctactatg
    15541 aatcttctgg cttggctttc atttctctta tgtaatcatt ttcttttata caacttaaaa
    15601 aaaaattaaa ccattagcat aatattttct gaatctattc acgaaagtct ttttctatct
    15661 ttcgttttcc acgactcaac tgatctagag tcaaaaaact aaaacgacag ttgcgcaaat
    15721 gacgcacaag ctgccgcttt aataacaaat agcctttcat tctcagcagg ccacttattt
    15781 ccagttaact ttctgttctt agctttttac tcaactggat aatatatgca cttaatgaag
    15841 tcccaatttc cgaatgttga agtccatatt caatatttgt cttcatatat cctaacttat
    15901 cgcctacgtc gtatcttttt cctttaaact ctctcgcata gaccgcctct gtctggttaa
    15961 gagtctgaag tgcatccgtt agctgtatct cgtttcctgc tcctggttct tgagtttcta
    16021 atatctcaaa tattttagga gtcagtagat aacgtccgat aatggctaaa ttacttggtg
    16081 cttttcccat ttcaggtttt tctacaaacc cttttactct gcaacgaccc ttttcgctat
    16141 ctgttttagg atcaataatt ccgtatttag atgtttcatt ttctggtaca cgcatgacgg
    16201 caacagcgga agcttgtgtt tcttcataat ccatcatcaa ttgttgagtc aatgggatat
    16261 catctttcat caaatcatcc cccagcatga cgacaaacgg ttcatttcct ataaatgctt
    16321 ttgcttgaag cacggcatcc cccagtcctc ttggatgaga ttgacggatg aagtgaagat
    16381 tgacttgagt cgtttcttca actaaagcga gtaattctgt tttttccttt tcccgcaaat
    16441 tgttttccaa ttcgatattt gcatcaaagt gatcttcaat gggtcttttt tcttttcccg
    16501 ttacgatcaa gatgtcttcg attcccgaat ccaatgcttc ttccacaata aactggatcg
    16561 ttggtttatc cacgattgga agcatttctt ttgccatggc ttttgttgcc ggcaaaaaac
    16621 gtgttcctag cccagcggca ggaatgactg cttttttgac cttcacttgc t
//
LOCUS       DARBDV010000066        16444 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000066 DARBDV010000000
VERSION     DARBDV010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.066"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            1..1404
                     /locus_tag="SCM56_002015"
     CDS             1..1404
                     /locus_tag="SCM56_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEG1110772.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTISS
                     TESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPI
                     LSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQA
                     PNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGI
                     SGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGP
                     QMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSW
                     GNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDS
                     SLSQSMLVDPKTLLFGD"
     gene            1753..2385
                     /locus_tag="SCM56_002016"
     CDS             1753..2385
                     /locus_tag="SCM56_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HEG1110773.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            2973..4412
                     /locus_tag="SCM56_002017"
     CDS             2973..4412
                     /locus_tag="SCM56_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1110774.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4707..4925
                     /locus_tag="SCM56_002018"
                     /pseudo
     CDS             4707..4925
                     /locus_tag="SCM56_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5174..5389
                     /locus_tag="SCM56_002019"
     CDS             5174..5389
                     /locus_tag="SCM56_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110775.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5606..5862)
                     /locus_tag="SCM56_002020"
                     /pseudo
     CDS             complement(5606..5862)
                     /locus_tag="SCM56_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6049..6960)
                     /locus_tag="SCM56_002021"
     CDS             complement(6049..6960)
                     /locus_tag="SCM56_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1110776.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(6979..7983)
                     /locus_tag="SCM56_002022"
     CDS             complement(6979..7983)
                     /locus_tag="SCM56_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEG1110777.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(7980..10103)
                     /locus_tag="SCM56_002023"
     CDS             complement(7980..10103)
                     /locus_tag="SCM56_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEG1110778.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10108..12555)
                     /locus_tag="SCM56_002024"
     CDS             complement(10108..12555)
                     /locus_tag="SCM56_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG1110779.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12542..12931)
                     /locus_tag="SCM56_002025"
     CDS             complement(12542..12931)
                     /locus_tag="SCM56_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1110780.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(12991..13494)
                     /locus_tag="SCM56_002026"
     CDS             complement(12991..13494)
                     /locus_tag="SCM56_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEG1110781.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13507..13731)
                     /locus_tag="SCM56_002027"
     CDS             complement(13507..13731)
                     /locus_tag="SCM56_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110782.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(13835..15745)
                     /gene="ltrA"
                     /locus_tag="SCM56_002028"
     CDS             complement(13835..15745)
                     /gene="ltrA"
                     /locus_tag="SCM56_002028"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEG1110783.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         4896 a         3260 c         2931 g         5357 t
ORIGIN      
        1 atgaaaaaat ctttaaaact tgcatcagca gttatattaa tgtcccaact ccttatagca
       61 acacctttcc atgtgttggc aagcgaaaat tcaacataca aaattgcaaa agaaagaacc
      121 gagacgactg aaatgagtaa ttccgatgta acagcagaga gtagtgaact tttgacaact
      181 tcatcaagtg aaagtagtac gacttcaaat gccgagtctt caacaacttc atcaagtgaa
      241 agtagtacga cttcaaatgc cgagtcttca acaacttcgt caagtgaaag tagtacgatt
      301 tcaagtaccg aatcttcaac aacttcaagt agtacaagtg aaataacttc ttcgaaagtg
      361 acaaaaccaa accatataga atcttcaaaa aaggaagcgc caaaaacgga aggacaaaca
      421 aaaacttttt cgacaccaca gcaaatagaa tcaacagaat ctttccaatc acaaccaata
      481 ctatctggta atgaagaaat tcattttgac aaaaatgaat ctgtagaaaa ttttattatg
      541 aaaattggtg agtctgcgag aaaaattggt cagaaatatg atttatatgc ttctgttatg
      601 attgcacaag cgatcttaga atctgccagt ggtcaaagcc agttagctca ggcaccgaat
      661 tacaatttat ttgggataaa aggaacttat aatggaaact ttgtcattat ggtaacaaac
      721 gaagatttgg gtaatggaac gctttatacg acccaatcaa aatttagagt gtatgaaaac
      781 tatgaggaaa gctttgaaga ttatgccaaa ttattaacta agggaattag tggaaataaa
      841 gatttttatg caggagcatt aaaagctaat agtaaaacat atcgagaagc aactaaattc
      901 cttacaggac ggtatgcaac agatacacaa tactatttga agctaaatga attaattaaa
      961 acctacgatt taaccaacta tgataaagaa gttaaaggtc cacaaatgag taagactgga
     1021 taccaagtgc ctattcataa ttattcaata accagtttgt ttggaaatcg tggaaatgaa
     1081 tttcaccgag gacttgatat ggcagctaca cagggtgagc cgattcatgc agttaaagct
     1141 ggtaaggttg ttaaagcaga gtttcactac tcttggggaa attacgtggt gatacaacac
     1201 gaagatggat caaccgcgtt atatgctcac cagcaacaat acctagtaaa aactggagat
     1261 aatgttttgc aaggacaaat aattggctat gtaggaagta ctgggaatag tactggccca
     1321 caccttcatt tggaaatttg caaagatagt agtctttctc agagcatgtt agttgatccg
     1381 aaaaccttat tattcggaga ctaaaaaagg taatcaatct gacaaagtaa caagaaaatg
     1441 gttggattct tagacctttg atcaggatat tgagactgga attgatatct cgattacgat
     1501 gggtttgaca aataaaacaa gtccattatc gaatatcgag tgtattattg ccgtctttat
     1561 gctcatattt tgagcaggtt tcactagatg aaaacaattt atctatcttg aggatttcgt
     1621 gtacgtatcg ttcagtcttg tattgtaaat tagtcataaa atcagactaa gaaaaattgg
     1681 aaatattttt ttctgattta ttaaaagtta attgaaatta tatggtactt ctttcttggg
     1741 agggtaggtg cagtgaagga aagatatggt caccttactg ttgttgagga gattactcat
     1801 aatggatata aaaaatatcg ttgcctatgt gactgtggga atgaaacgat tgtatatcga
     1861 tcaaacttac tatcaggtag aaccaaaagt tgtggatgtg gaaattataa aaataggcat
     1921 aaatataaaa atttgacaaa taaaactttt ggtaagttag ttgcaatcaa gccaactaaa
     1981 ctgcgaagtg aaggaactgt tgtttgggaa tgtcaatgtc tgtgtgggag aaaaaagtta
     2041 gtatcggcaa agcgacttct aaaaggtgat gtaaaaagct gtggatgttt gaaatcaaaa
     2101 aagcatcaca ttgaaaataa aaaattcgga ttacttactg caaagtatcc tttggacaat
     2161 aactgttcat ggaaaacaaa atgggtttgt cattgtgagt gtggcagtat gtgtgagata
     2221 agttatagta acttattttt tggacatact caaagttgtg gatgtttaaa aaggcaagaa
     2281 acaaggacat gtgttgaagg gacagtaata gagtgtctaa cgtcaaaatt accgaaaaat
     2341 aatacaagtg gtgttaaggg agtttatcgt tctaaagaga aatagattgc gtatataact
     2401 ttaaaaaaaa agaggtacta tttaggatcg tatgattcta ttaagagtgc agctcaagct
     2461 agaaaagatg ctgaaaataa attgtttcaa cctttgattg acaaatatcg aggtaatcat
     2521 taattattgt ggataataat taacagtgat atataaacat ctataatata ttattcatac
     2581 tattgatatt tagtataatc ctatggaatt gaatatcata gcaatgtatt tgaaaagcaa
     2641 attggaaaac aaataggtgc aacaccataa atatgaaaaa attgggaaaa agtagaatgg
     2701 aaatttgctt acgtctcacg agatagctca cactattaat ctgaagaaca atgaagtcga
     2761 cttttagaaa ttgctagaag aaagtaaagc gaattatcac tagttactat agagtaagtt
     2821 caaataaagc atctccaact tacatggtta catgaggata tcaaatcgaa attactcaag
     2881 cttcctatgg ctagagtcgc gagaagttgt tgggcagtag acataggtta tattgttctt
     2941 agctacacga tcattagaaa ggaaatccta ttatgtatag ttatcaagaa gatgcaaaag
     3001 tccaaaaaaa tcgctggtgg attttgattg ctatatctat gtttaccttc atgtccacat
     3061 tggatggcag tattgtcaat attgcactac ctaccatctc agatgatatg ggcataccaa
     3121 tgaatcaagc ggaatgggta gtgtccgttt atctgatgat tgtctgttct tgtttgttgc
     3181 tgtttggtaa aattggcgat agccgtggga aaatcaaagt atttcggatt ggtacgctga
     3241 tctttgtctt gggatcgctg ttgtgtgggt tcaatcattc gctaggtatt ttattgttcg
     3301 cacgggtggt acaagccatt ggtgctagta tgactatggc tacaaatgcc ggaatcatca
     3361 cggaagtctt tcccatacat gaacgtggtc gggcattggg agcaataggt gcgtttgttt
     3421 cattaggctc gattgctgga ccaggtcttg gcggcttgat tttgtcccaa ttttcttggg
     3481 cgtacatttt ttggatcaat gtaccggtgg gaatcgtaac gatgttgatt gctgaaaaat
     3541 tcttaccaaa agatatcacc atgagcggcc atacaatcga tggagggggc tttgctgcca
     3601 ttgcggctac gattatcagc ttttttggag caatcttttt ggggcaagaa agtggctttt
     3661 tccatccagt cccccttgta ctgttcgtaa tttcagtgat tgcctttgtc agctttttaa
     3721 aagttgaaaa gaaagcagcc tctccattga tttctttcgg tatttttaag aacaagattt
     3781 ttaccatgag cttgattaca gccttgttga ttttttcttc caacttcttt gtcaatgtag
     3841 tgattccttt ttatctgcaa aatgctcgcg ggttgagtcc tagctatgct gggctgttga
     3901 tgatggtctt tcctttgatg atggtcgtcg gggcgccttt aagcggctac ctgaccgacc
     3961 gggttggccc ggagattttg gtattagtag gcttgggctt attgtcggta acccaggtca
     4021 tgtatatgtt tttgtcagtg gactctcctt tgtggctata cattgtcgct actgccatca
     4081 tgggtgccgg caatgccttg ttccagtccc ccaataatac gatggttatg agctcggtca
     4141 ctatcgaaaa cttgggactg gcaggctcga tgaattcctt tgcccgaaat ttggggatgg
     4201 tcagcgggat ttcgctttct acgacggtct tatatcaagc gatgtctgct aagatgggcc
     4261 agcaagtaac taactatatc gcaaatcggc cggatgtctt tttatatgga atgaagatta
     4321 cctttttagg cagctttgtt ttgtgttttg ttgccttttt cctgactatc tggcgtatca
     4381 aaaaacaagg aggtattcag aaaaatgtct gaaaaaacca ctgttgaaga atttttggag
     4441 gcctgcgtat gggtgagtgg gatttccatg gatgatggaa gatgaagtga acttctaaaa
     4501 gccgaaaaac tcaagtatta tcttcaaaat actagtgctc aatgagatgc ttgatttgat
     4561 cacaagaaga ctgcgaaagc cgctggcaac cgcaagctag gtatcccacc gatggactac
     4621 gttttagagg ctactggtta tgaatacgga gccaacgctc tcatctgcat aagaatacat
     4681 cacccagatt atgtaaagat tttcgatact tctgtcaaaa aatcattaag atagataaat
     4741 gatttccgtc tagtcaaatt ggttcagaat gtagacacac agatggtaag aagtcactca
     4801 caattcgaga atggacttgt cctatttgcc gtatacatca taatcgaaac gtcaacgata
     4861 gtatcaatat tttgaccaag ggctatgaat acaagcattg ccttagaaaa catcaagaac
     4921 cttaggaaca acggggatag cttggttaat aagagatacc gctgtttagg cttgcaagta
     4981 tactctttta tcaagaattc cgtggcttta gtcatgagag gcttaatact atgaaaattt
     5041 tctttagcat ttttgattta ggttcctttt ctaattataa taagatgacc actacagtaa
     5101 cctactgaaa tatataaaca gccactgtag taccattttc aaagtagtct atgagaggag
     5161 aatcttcatt cttatgaacg ctggaattgc ggatatgaat ttaataaaaa aaacccttaa
     5221 tgattttact tccaattcaa tatcaaaagg tactggtatc aatttaagca caataaaaaa
     5281 gctgaaaagt ggtgaaagat cagtggaaaa gctgaatctt ttagatgcta taaaaataac
     5341 ggaatttgct atgaaaaatg gaaaagctga gattgaaata tggagataat tatcgtctag
     5401 tgaattactt gccacttagc tgaatttaac gatccttggt gcttgaaggg ggagattctt
     5461 gggaatagag catacttgca agcctatgtc tttactaaca acgatatctc ttattaacca
     5521 agctatcccc gtagttctaa ggcttttact tttatctaag caataatcgt attcaaagga
     5581 aatttttgac ccttagaaag tctcatttta tcagacgtga gcttaggcat tgctgaattt
     5641 tgttaattcg gttttaggtt gtgctttgag cataatatga aagtattctt tatcacgatt
     5701 tcattcatct aaggtaatgt gatgtgattc tgaaattctt tcaaaggcag tttttgcata
     5761 agctgacatt acatcatcaa tgacatgttt acaatgtttc actactaaaa taagggggta
     5821 accaagaaga aaaactgaat gattatttgt atctaattcc atggtatatc aaccatttcc
     5881 tatatagact gattatgaca tggattaaaa taaaaaggca ctatgtctta attgtcggtt
     5941 ttcgagttac cgaatggcat tcatcaccca cctatagagg atgggcgact tctgcctgaa
     6001 ataagttaca ctacttataa cttctaataa aattagattg agtcattatt atttaacatc
     6061 aacgattttc cagtttccat ttttttgtaa tactaatttg tactgaaagt agctagtggt
     6121 ttttgataca ttatcaaggt attttatctt tgtactcact agaaaatttt ttccgttttg
     6181 tagataaact ggattctcta actccgaaaa ctttaagtca atattgattg gtcgcatcgc
     6241 accattttca acataatact ccaattcttt ttcagaagct gatggataaa gtttaaaaaa
     6301 tgtttctaaa aaatcggtga tttcttccgt tgttttggca tcaatttctg aatcgttttc
     6361 tacttgtttt gcttcatatt ttgccttgtc tggctttcca atcattgttg gcaaactagt
     6421 tactaaatga ttcctgtttt catcttcaaa gattgtcact cgatatgcag aagaaattga
     6481 ttgcgtcttt tttccctctg taatatgttg ctccactaaa aatgaaacga caaattcttc
     6541 cgagtgtttt tcaacatcta gaatttttac agattgaact tcggaactcg tgggaatatc
     6601 tgctcgaacc atatcttgga ttaaagcgag accgttttct gtaaggtact gttcgagatt
     6661 actcattctc ttttcaattg cttctttgtt atttttccat gaaaaatatt ctttcgcaaa
     6721 atcctctgta aaactttcaa cgccagaagt attcactacc ttctctttta taaccacttt
     6781 ttcatgagtt gtatgttgat caatggcggt gaaattttta taaacgccaa aagcaagact
     6841 acaagacaat aaaagccaaa gggcaagcac catttttcga tgcttaccca cggatacaac
     6901 tcgttctttt ttcttttttg ggctcttttc cttttgattc cgttcaattt ttatcttcat
     6961 tctttttcct cttttctctt atttgtatcg gccagctccg ataagatgct gccgccaata
     7021 gttttccgtt agattcgtcc accccactgg atctcctgca tgaaacatgc gattctcccc
     7081 tgcgtaaatg cccacatggg tcacataagt accggcatca taggttgaat gaaagaaaac
     7141 taaatcacct ggtttggcat cttttagatc aatatgagtc atggcatcat attgatcctg
     7201 agccacccga ggaagtttca ggtcaacttt ggcataacac cattgggtca agccactaca
     7261 atcaaacccc gtttctggac ttgagccacc aaacacatag gccgttcctt catacttaaa
     7321 tgcctcatca aaaatttctt ggactatttt gtcatcaaac tgagttgtag ttaggtattg
     7381 cttcactaat aaacagtaaa acatattgcc ataattatac cgccacccac cattttcctt
     7441 aatcgcgatt gggttggggt aatcgacttt ttgaccacct gatttttcct ttgaaaaaga
     7501 ttcagctaaa gcaaaactat gcttcttccc gtttttcccg acatgatcta gatacccgcc
     7561 accatagtta taggcctgaa taatactatc ttgatcacac cctagttctt tggaacgctt
     7621 aaccaaagac gcaaaataga gacagccttg tttgatggat gcctcggtac ttaatgaatt
     7681 aggtggtaat ccggctgact cagaactttg cataacgtct tccattgtgc caccagattc
     7741 cacttgaata atcgccaaaa gaattggaat atactctgtt atatccgatt ctttggcata
     7801 cttttccacc attgcacggt gtttcaatac ttcttctgag acactagttc cgccaggaga
     7861 agtgccagaa ctaccatttc cctcactgtc ttcaccaaac aacaaaccca cacacatcaa
     7921 aatacccata aaagagatgg tcagaaaagt taatgaaata acacccactt tcttcaagtt
     7981 cattttttct cacctgacct tgattttgga cgatggttca ttcttttttg tactttttga
     8041 ttggtctgct ttggcagagt ggacctctgt ttcacttgtt ggaaagaatt tttcttactt
     8101 gctgattggc ttttcccttt tgaaatgatt tcttgactag ttagttttcg tttgatttcg
     8161 ggattcagat ttgctctcac aatccgcttt ggtgcttctt ttttcagcat catatctttc
     8221 tgaggttgag ctgctttatc tattggaata ttcgcccttc tttttccctg acgatttgtt
     8281 gcttcactca gtatcttgcg tttttcgtcc attttcgcat ttcttcgttg tcgttgtttt
     8341 tctcgcaaat ccactcgatc cgctttttct tggaacagtc cgcgcttgaa ttcttctggt
     8401 gcttttttcg acttttcgat tcctttatgg agattgtatt tcaaattcgt tggcgtattc
     8461 cttacctgat cctttacttg tttagtctta tctttcacca aagcttgggt atccaaaact
     8521 ttgccgattt ttctcccagc caaattcatt cgggattgtt tcttattatt tgaaactgga
     8581 ttctccttag ttaagtcaga tagtatttcg tgatcgttcg gtaatcgtga attctttcgt
     8641 agagaagaac cggatggctt aaatttggat tttgattgtt cctttgccaa cttatttccg
     8701 actgctgcac caccaccaaa agccaaagct cgtcccacat ttcgattgag ttgccgcaat
     8761 tttcgattca ttaacatttg cggttttcgc atgacgcgtc ttcccattga ctggctatca
     8821 ttactctgga ggttgaacat actcataatg tcgcccaatt tgaaatagat accggcaaag
     8881 gtcacaattt gcagaaaagc aaccatgaaa aatggatagg ttgccgaaat attgaaaaac
     8941 atcgtggaga tgctaaatgc agtcgtaatc accaaagtaa cacccgctcg catcataatc
     9001 gtattgaata aacgaataat cgccttcttt cctaaacttt cataagtcgg caacatggac
     9061 aataagaaac taattggcag aaacatcgca aagataatga agagaatttg cgagaacaac
     9121 ataatccctg ttagaagaaa aacaaagaag gaaatgccaa tattgaacaa taccaaaaag
     9181 accacggtcc ctaaacggtt catggtcttg gtaatactga gattcgcatt gtcattgtct
     9241 tcaatctctg ccttgaccgc ttcttcccga tctttgcctt tgttttcatc tggactcaca
     9301 gatagaatct tgttgacccg gtcttcacca atttcttcta tgttggaatc atcaaattgc
     9361 agtagtaacc aaggttgttg gacttgaatc gaaaacaaac tatcccgaat caaatccaca
     9421 ctatcttttc cttggctttc tgaattgggt acaacgatct cggttccaag ggtaagggcc
     9481 gcttcactaa catctgagct aaagtcattg atttttgtga tataggtagg cgcataagca
     9541 atgaacgaac ccgatagtag aaaaatcatc acaaaattaa tcacggctcg aactgctttc
     9601 gtggtttctc gttttaataa tccggtatac gccacataca cccccagagc taaaatcatc
     9661 aataatagaa acccaaaata aaatccggag gtctggagtc cattttcagt aataccagcc
     9721 aatgtttgga tattctttcc aatactttca gccgtatccg aaatgaaatc taatttgtaa
     9781 gcctcttgaa ccacataacc cgtggcgttc gacaaataca aactcaccgt ccaaatgaaa
     9841 ttagtaatgg cataaagacc atacatcacg cttttcccta agccatcacc ccaattccaa
     9901 ggcaaccaat cccaagagct atccacaaag aaatctagtt gatagttgtt cagtgaatat
     9961 ttggaatata gatttcctgc ttcaactgta tcatcaacta gccctgtcgc ctcggctacc
    10021 gtaccaacta aactcaatag aactatcaga gtgagtatgc taataaaaac gattcccaca
    10081 atccgaaaaa gtttcttttt catcccctca ctccattctc tcttgaacag gtggacgggt
    10141 atcaaaggca tggaagagtt cttcaaagac tggatgtacc tgaaccacgc ctacccgacc
    10201 atacaaatcc tgcatcaagc attggccatt ttccaaatct cgtaggcgtt tttgattgct
    10261 ttcatcttct cgatccaagc caaagaattc caaggttttc ttgatttcat gaatatcggt
    10321 actgcgaaac gcaaacttca acccgatatt attcttcaag cgttcatcca gtaaatcatc
    10381 actattttgc gtgacaaagt aaaccccggc attcatagcc cgcccagcac gaacgagctt
    10441 attcgataat gttttccctt gcgctacttg aagaaaactc cacgcctcat ctaagtctac
    10501 aattttgaag atttctcgat tactatggat aaagtccaag gcaaaagtgg aaatgacgat
    10561 tagcatggaa acactaagca attccatggt ggtatattct tcaaaagtgg tttctgcatc
    10621 tggtaaaacc aaatccgcta cttgaatgat atttaactgc ctatctaaac taatcgactg
    10681 ggtaatcgaa ccatctgaga aaagcaaatg ggcaaaatca taatcggtaa aggactcgat
    10741 gtgatccgcg atattttccg caactggtga gccttctttt cgaagctcat taattacttg
    10801 taataatccc cgttgattgc tttgggtaac cgtccgaatg gccttacgca acactggaaa
    10861 cttctcccca tctcgactag aaattcctgt taaaaaggtc agaatatcaa ttgctagact
    10921 ttcagaatcc ttctttttct ccataatcac ataaggatca agtaatcctt gattttttgg
    10981 cttactagtt aagttgagaa tattgatttc atccgcaatt tctggcaagg tttctttcca
    11041 gccaccccgt tccccttttg gatcgacaat cacagcttgt ccaccaaaaa ggacggcgta
    11101 ataaactaag agattgttac taaaggactt tccgccaccg agtgaaccga ggaatgccgc
    11161 tgctagcgca ttggtaactg aatccttgac cccttgtgag gccaatgccg gtttcaggta
    11221 aacattgcgc cccgtatcta cgttataacc aaagtaaatg ccttccaact cccctaacat
    11281 ctgagtggcg ccaaaaccaa gtccggcgag aaaatcagac gtaacatact ggatataatc
    11341 attcatgtat cgcttactag aaggtaagaa ttcttcatgt agccccatca tatcgccaaa
    11401 aggccggact agttttacat tggtatcatc ataaaaatct agcacttcat cacaccggcg
    11461 ctttagttca tctactgatt cggcacttac tcgaaccaca tagctcagtt tgtacatgga
    11521 ttctttggac tgatccaagg tagtttccaa ttcatctacg gaatccaatg catctaagac
    11581 attggaattt gtttcattgt cagattgata ggcatggttg tccaagtctt ttaattcctt
    11641 tttcttattg cgaacggtag caagtgcctt tttgttcgtc acaatctcca cattcatact
    11701 agtgtcaatt gggaatgtaa attgttgttg ctggtaatag aaaagttctg aggagggaaa
    11761 ctccatttcc cccacaattg tattaattgt taagtacgct acatatgatt catgattctc
    11821 atgttccatg cataagtacc ggggcttttc ttcaatcaaa caacgactgg gacgtaataa
    11881 gtcgtaacgt ttcaccaaag tttccgactt cagctttttc tttggtagct gaaattcata
    11941 ttcttcaaat ggtgtaccct tttcgccata gatatgttca atcaaataca tcagatcact
    12001 cggtgtgact cgtctgactt taaatcttcg agccaattta ctttccatta gtttttcaag
    12061 cttggtatat cggcgaattt cttcattgga taaggaaaca aaatcgccca tcaaatgatg
    12121 attcactcca tagacaaatt cttgaaagcc ggagaagaaa gatttcttga gattctttag
    12181 attgacctct tcatctgtcg caatcagttt gaagccaata aaaaagcgat aatcaatctg
    12241 actatcgcca atcatggata ctaatgcctc tgtctgtaaa tcaattcgtt gtttcgctac
    12301 ttctgttaat cgcccggtaa tttccttttt tgatcgttct tgcgtggcac gaatactact
    12361 ttctgtggca atttgtaagg catgaatctt gccttcacga ttttgcgcaa tcaattgacg
    12421 aaagttatcg tggacttgat atttctgttc aggtgacaaa aaggagtaat tgtaaggtat
    12481 taactcatag taggcaaaac attctccctc ttgattaaag acaagattat tttcaatata
    12541 cttaatcggg tacatagttc acactcctta ccagagtaat gggttcgtcc catcgtttct
    12601 tctgttcttt gactttcttt cctgcgaaag tgaccttaaa tcgtaagtga tagctaataa
    12661 aagatcgtaa aaatcccaat ggtttcttac cgtcaaacgt tttttgcgac atgaaccaag
    12721 taactgccac cggtatacct aagtatttta aaaaggcccc ctctatcata gatagtggag
    12781 gcacatgggc aaatacgatg acaaacagca aggataaaac aaaccaagcc atctggttaa
    12841 aagtcacagg aaaaggcagt tgaaagtcat taattgcata aatgaccttt tcaactgccc
    12901 aaatactggt gtagcttttt attttcttca tcaaatcatt cctttaaaaa gaaggatagt
    12961 ttagaaatca actacccttc aataattttg ttaatatgga tactcaaaaa cacctcgact
    13021 cgttaccaaa taattgcctc caatctccaa gtctctacca cacgcatcaa aatcaatata
    13081 cgtcttcaag tgttctggta cttctccaaa atcaccagct tctacaatca agaaatatgc
    13141 tacatcactc atatcatcgc aatctggata atagataata tcatccacat gctctaccat
    13201 ctcctcaaaa gaattgaaga aagcatgttg aatctcagaa gcaacttcac caactggcgt
    13261 tccttcaagt tcctccgcta agccacacaa ccgattaatt tcctcaattg gcgtatactc
    13321 atcaatctca aagggaagtt cataatcatg aattgcatat tcctcgtatt cgtcatttaa
    13381 cccaattcgt tctttcactt catcaaaatc gacaggtggg gtaaaccacg cccccaccaa
    13441 ttcaccttcg ttatatttcc cgaggttcgc aatataaaca cgcatctgtt ccatcgtgct
    13501 tcccccttac gcaccgacaa ttcggttaaa caaactgagt aagacatcct tcaccccaga
    13561 agcattaaag accaatccga ccgcaatcaa agcaatcacc aaaaagccaa tcagtttgga
    13621 aaactcccgc ttaaatccta aaaacaaacc aatgaccaca attgccagta agactaagga
    13681 ttgggcattg gttaaaaacc attgatatat ggataggtaa gttcctgata ttataaatat
    13741 ctcaaacttt tccccccatt cacacggagc gtgcgacttt caccgcactc cgcgttccat
    13801 cgtgtttata ttgaaaagta tttatgtagt agacctatat caagtttaat tcttgattgc
    13861 cagctaatac tctatattta tttagtctta tgagctggac tttatttagc ttcaatagag
    13921 ctatatattt ttgtattgtt tcttctgttt tagcgtgtat tagacggtgt accgcctctt
    13981 ttaccaaaat aagattttta taatcgtcat ttccaccagc tccagttggt acaatatgat
    14041 gacaatgtat ttcattagct tgttgaaatt cttccagagt tatagcacat tttccatgtt
    14101 gagcagaaaa tagcgaaatt cgattgtctg cgtattctat tgtgcgattt tccaaagata
    14161 atttcattaa ctggtacatc agtaatttat caacacttag attggtgtgg ataaattttc
    14221 ttccctctag agtgtagcaa ttcgctaaat acacctttgc cattggtttt ctgtgttgga
    14281 tatatccaac agggaaaata ggttcgccct cgccagcaac ataccgaagc attttagatt
    14341 taccatatct tgttgcctcc tttgtcgtaa gttgtctgcc tgtttttctc aatctgcccc
    14401 ttttgtttgt acctaatctg ttagtgaaga tagccattac agctctattc agtttcatac
    14461 aatccagatt tacattcgtt gcaatctgat aataattttg catacctaat accattgaat
    14521 tataaagtct tgtttcttct aattccgttt tcccttttga gggagaagca attttctttg
    14581 cctgtgatac cagcttgtct ttttggtttt tgaattgttt atcacttata tgtgatttaa
    14641 caactaactt ctttcttctc ggtatcattt taatcttgaa accaagaaag tcggagtatc
    14701 tttttctaac atttacaatt cttgtttttt cttgtgatac ttccaatttt aatcgttgtt
    14761 caatccattg agtgattgct attttggctt tttcagcact ttccttgtag cggcagaaaa
    14821 ctctaaaatc atctgcataa cgaaccatat acatttcctt taactttgtt ttcttcattt
    14881 ctttgtaagc gttgctttta catgggctac cgcttttatt gatatgaacc acatagtttt
    14941 ttgtaacagg attttcctgc cattggcttt ctacccaatg gtctaactca ttaagaacaa
    15001 tgtttgctag tagtggggat attattcccc cttgcggagt tcctttctct ggatatgtta
    15061 ttgtaccgtt agtcattttg attggagctt ttaatatcct ttttaaaatg aatatcaagt
    15121 gcttgtcccg tattcccata gcccatatct gttttatcag cttggaatgg ttcacgttgt
    15181 caaagaaacc cttaatatca aattcaatca cataatgtag ctggctgatt tgtaggcggt
    15241 tataggttgc ttttattgca ttttcaactg acctattagg tctaaaacca tagctgtttt
    15301 cactaaattt agcttcgcag attggttcta atacttgttt tatacattgt tgtataagcc
    15361 tgtcccacat acaaggaata cctagaggtc tggttttacc atttggtttt ggtatttctt
    15421 ttcgcctcac tggcttaggt cgatacccat gtggactgcc tgcaacaatg tatcttactt
    15481 ttttgacaac tgtttctgct ggcaatttgc caatatcttt aattattgta ccgtctgttc
    15541 ctgctgtttt actgcctgca tttgctttga tatttcgata tgccagtaat atattttctc
    15601 tcgataggat aatatccata agatgagtga aaatatctcc attcttactt ttggcatata
    15661 aatcatcaaa ggtgttttgc atttcgtaat attcagcgtg tcttaaatct tctacacaca
    15721 atgtttcatt tttattttta ggcataaggc ttcactcctt tacttgaaat tgagtgagct
    15781 tttttatctt actcgtaatc cttattgaaa caacataata cttttctaat atatacacga
    15841 cttgagggca ttcctccatt ctcattacaa gaatttctta ggttcgccct ctgcttttca
    15901 gcattagttc aactgcttat tgttcttcgt cagtttattc tcttgatgag aacccaatac
    15961 ctttcctcgt tccgataatc ttatctatac atatatatcc ttaggtcact agctataagc
    16021 ctgctggctt gataccgcct gtaacgttat atggcatttc ataacctata ttcttacttt
    16081 gccacaccag ctacgatttc tcgtaccttt gcatttctac aaagttcaaa tttagacccg
    16141 tacattcgct gtttcgtcag ttccttatag aacattctaa ccatagatat tttatagacc
    16201 cccgacctgt tggatacgct acccacctct ggatagtttt cgatagaaga tatatgttca
    16261 tcaactatta cggtatcttt cagccgtctt taccgagctt caaacattct gagtttcctc
    16321 atacaatgct tgtcggaggc tcagggcagg cgtttcagag cgttacttca tcattccacc
    16381 tgtcagatta tcagttctcc taataaccaa actttttcgt tcttttatta gtgccttagc
    16441 tttt
//
LOCUS       DARBDV010000067        16129 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000067 DARBDV010000000
VERSION     DARBDV010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.067"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..468)
                     /locus_tag="SCM56_002029"
     CDS             complement(<1..468)
                     /locus_tag="SCM56_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEG1110784.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFN"
     gene            complement(598..1308)
                     /gene="budA"
                     /locus_tag="SCM56_002030"
     CDS             complement(598..1308)
                     /gene="budA"
                     /locus_tag="SCM56_002030"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEG1110785.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1321..2985)
                     /gene="alsS"
                     /locus_tag="SCM56_002031"
     CDS             complement(1321..2985)
                     /gene="alsS"
                     /locus_tag="SCM56_002031"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HEG1110786.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3193..3855)
                     /locus_tag="SCM56_002032"
     CDS             complement(3193..3855)
                     /locus_tag="SCM56_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEG1110787.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(3865..4680)
                     /locus_tag="SCM56_002033"
     CDS             complement(3865..4680)
                     /locus_tag="SCM56_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1110788.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(4941..5384)
                     /locus_tag="SCM56_002034"
     CDS             complement(4941..5384)
                     /locus_tag="SCM56_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HEG1110789.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5518..5856)
                     /locus_tag="SCM56_002035"
                     /pseudo
     CDS             complement(5518..5856)
                     /locus_tag="SCM56_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5844..6221)
                     /locus_tag="SCM56_002036"
     CDS             complement(5844..6221)
                     /locus_tag="SCM56_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEG1110790.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6445..7638
                     /locus_tag="SCM56_002037"
     CDS             6445..7638
                     /locus_tag="SCM56_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1110791.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(7804..8226)
                     /locus_tag="SCM56_002038"
     CDS             complement(7804..8226)
                     /locus_tag="SCM56_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1110792.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            8809..9303
                     /locus_tag="SCM56_002039"
     CDS             8809..9303
                     /locus_tag="SCM56_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110793.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQAA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(9465..10637)
                     /locus_tag="SCM56_002040"
     CDS             complement(9465..10637)
                     /locus_tag="SCM56_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEG1110794.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(10843..11658)
                     /locus_tag="SCM56_002041"
     CDS             complement(10843..11658)
                     /locus_tag="SCM56_002041"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEG1110795.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(11808..12254)
                     /locus_tag="SCM56_002042"
     CDS             complement(11808..12254)
                     /locus_tag="SCM56_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HEG1110796.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(12251..13255)
                     /locus_tag="SCM56_002043"
     CDS             complement(12251..13255)
                     /locus_tag="SCM56_002043"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEG1110797.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(13295..13624)
                     /locus_tag="SCM56_002044"
     CDS             complement(13295..13624)
                     /locus_tag="SCM56_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HEG1110798.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(13750..16077)
                     /locus_tag="SCM56_002045"
     CDS             complement(13750..16077)
                     /locus_tag="SCM56_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG1110799.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         4605 a         3230 c         2870 g         5424 t
ORIGIN      
        1 attaaaattt gctaataaaa gatctacata tttttctaga aatgcttgat cgatttcatc
       61 ataccctttc gttactccgc aatcgatatc taaaacaccg attaaattac cttcttttac
      121 cattggcact acgatttctg attgtgcggc tgaatcgcag gaaatgtaat ttgcatgggt
      181 tttgacatca tcaacgatca atgtttcttg tttttcagct gattctccac aaactccttt
      241 tcccatcttg atacgtgtgc aagaaactcg cccttggaac ggtcctaaaa tcaattcatt
      301 accatcgaat aaataatatc ctgcaaagac agtctcaggc aaagtctcat tcaacaatgc
      361 agatgaattt gctaaatttg ctaaccagtt atcttctgct tctaataatc cttgttgctg
      421 taataataaa atttcgtatg catctttttt ctgtttttct gtccacatga taaaaaccgc
      481 tcctttttga tctatggaga agattatagc tcagtttttt tgtaaacgct aggtttcctc
      541 ctcaaaaaaa cgagatacgt caggttaatc accttgaacg catctcgctg tctttttcta
      601 ttctgcttgt tggatttctg ctgctaaatc agcataatca atcgtttcgt gaacaaaagt
      661 cggatcatca actggaaaat gctgtacaag ggacgagatg cggctaatct caacttttcc
      721 ttcagccact tcaaaatcca ttacatgtcc gccaaatgta tgtgtatcat cgataaaatg
      781 caggtgaaat cccgctgcag caactccttg gaataattca ggcgtaaaga acccgactaa
      841 tgttccttgg atattgcttt gttggaattc tggttgagac tctgaaattt ttgctagtct
      901 tctgtaaggt ttttcttgct tcggcatgat acggatgtgc atatttttga ataatccagt
      961 tatcttcact gccaaaaata ggttttcccc ggcttctctt actaatatgt cttttttcaa
     1021 ttgttctcca tctgcagttt ttgagacaga aaaactagta tctggcgtaa aatctgtaat
     1081 tgctgcatag ggggtaagtt cacttccatc taattgggca actgagccat ctgatcttcc
     1141 ttgataagct acgccatcta aaaagatgac ttcaccatca agcccatgca atgttcccaa
     1201 tcccagagtt cctttttcta atatatgagc aatcgtttct gttccatcca tcaagccggc
     1261 catcaaagct cctaatgtcc cgtgctggta taagattttt tcagtcatgt cgacacttcc
     1321 ttaatagaat tgatctggta agattgtttc tcccaatttc tcattatcac ggtaatcaat
     1381 cggaatatca ataattacgg gtccttctgt cgcaaatcct tcttctaaag ctttttctaa
     1441 ttcctctaca cttgtagctc ggattccttt agcgccaaaa gcttctgcat atttcacaaa
     1501 atcaactgga ccaaagtcta cacctgacgc ccgctgatat ttcatttttt cttggaactc
     1561 taccatattg tagtgtccat cattccaaat caaatgaacg atattcaatt ttttacgtac
     1621 cgccgtttct aaatcctggg cagagaataa aaatccccca tcgccggaaa ctgatacgat
     1681 ttgtgtattt ggacgcacca atgcagcaga aatcgcccat ggcaatgcaa caccaagcgt
     1741 ttgcatacca ttactgaata acaaatgacg aggttcataa cttctaaagt ggcgtgccat
     1801 ccaaatataa tgactaccta catctacggt aacagtcata tcatctgtta cttttgactg
     1861 aagcgtgtta ataacttcta aaggatgcaa gatacctgct tctccttttg attgaacaat
     1921 gtcccgctcc gttaattttg cttgaagaga tgctaaatat tcttttgcat cttctgacac
     1981 ttgttgaggc tctaatgaac ctgtcaataa atcaagagtg gctgagatat caccgatcaa
     2041 ttctctttca ggctgcataa aaggatcgat ttcagctggc gcttcatcga tcacaatgat
     2101 ccgcgcatct ttttccgcat tccagtttct tgcttcatat tcgattggat catagccaat
     2161 tgcaataact agatcacttc gtttcaaaag catatctcct ggttggtttc taaataaacc
     2221 cacacgacca aagaaatgcg cttctaattc acgtgagatc actccagctg cttggaatgt
     2281 ctctacaaca ggcaatgctg tcttttcaac taattgcctg atggctaacg tttctttttc
     2341 acttgatcct cgcataccta ctaatagtac cggtagtttt gcttctctaa tgcgttcagc
     2401 taggtaacgg atatcatctg cagaagcaga acctaattta ggatcgctca atggcttgat
     2461 gacattacct tggacaggtg catccactac atcttgcggg atactgataa agctcgcgcc
     2521 tttttttgaa ctcttagcca tacgataagc attggcaatc acttctgaaa ttgtttctgg
     2581 atcttggatt tctgcactgt attttgtgat tggctggaac agtgctgcat tatccatgct
     2641 ttgatgggtc aacttcaata gatcgcttcg ttttacttgt ccggcaattg ctaaaactgg
     2701 gtcaccttct gctgtcgctg ttaccaaacc tgtagctaag ttactagcac caggaccact
     2761 tgttgcgata actactcctg gctctcctgt aatacgtccg attgcttgtg ccataaatgc
     2821 agcattttgt tcgtgacgag tcacgattaa ttcaggacct tgatcctcta attcattaaa
     2881 tacaccatcg atttttgcac ctggtatacc gaatacataa tcaacatgat gattaatcaa
     2941 actttctact acagatttcg aaccttgttt cattttttca gccattgaac atcgtccttt
     3001 ttatgcttta tttttaatta ttagttagaa ataatcacaa ataagtgata cagtttctaa
     3061 acctgtttca tacaaaagta tagcacgttt tatttcattt gtttagccat atgtttcaaa
     3121 aaatgaaaaa gaatgacagt ttttacataa aaaaaactgt cattcttaat tgaaagtttc
     3181 aatttataaa gtctacagat ccatagttct catcaattta cgattgttga agtaaatcaa
     3241 accaaatacg atagaaggac ctagtagaat acaccattgg ggctttacga aaaagaagag
     3301 aaacaaactt gtcaatagta aaaaatctag taaactcaca taccattttt tcaacgtata
     3361 gtagaacgct atccgtagaa cttgatttgg tttcgacgct ggatttctca cctgaaaata
     3421 ccagcagttg gtgctaaagg aaactgataa acaagcaagt aaaacaaaca acggaatcaa
     3481 ccattttcca taagaaaaac gtataaaaaa gaaacaatca aaaatcatta tcacactaac
     3541 tatccaagca aatagccaat aaaaaaatcc ctttagccaa aattggcgaa acatctttat
     3601 gaacaaacgt actggtgaaa aactattttc ttttgctaat tcttctgtca tacctatcat
     3661 cacgatgcaa cctggaccaa taaaaagtaa agaaaaaata aaggaaaaga cattccttcc
     3721 atctatcgac aaaaggaacg cagctgctat atacaagata ttgacacacc aaaaacaaag
     3781 gctacttgcc aatgattgat acaaccaata aaagaattcc atataaacat ttttgtcaaa
     3841 acgctgctct ttcataatca cctcctattc cttcattcct gatgaagcga tcccttctac
     3901 aaaatatttt tgcatcgcta aaaatataaa agcaacagga agaacagaaa caactgaggc
     3961 agccatgatt aatgcgtact ctgcatcaaa tagcccaata aacattttca accctaactg
     4021 gattgtttta ttttccgtag aagacaaata aatgaatggc cccatataat cgttccatgt
     4081 attaacgaat gtgatgatcg tcaaactggc aatcgccggt tttgtaagag gtaaaatgat
     4141 cttgcgatag attccccact ctgataagcc atcgatccgc gcactctcac ataaagaatc
     4201 tgggatacta ctgtaatatt gcttcaacaa gaacacacca aaagcattga acgcttgtaa
     4261 tagcaccaac gaccacaacg tatctgttaa acccactttt ctcatcataa tgaactgcgg
     4321 gatcatataa gactgccaag ggacagcaat cgtgccgata tatgctaaaa ataaaatatc
     4381 gcgcccttta aaatttagtt tagaaaaccc gtaagcagca aaactagaag taaacaactg
     4441 aatcaacgtg atcacaacgc ttaaaaaagc tgtattctta aaaaatgtca ctaaaggaat
     4501 acgagaccaa ataaccagat aattctctaa atgccaagtt ttagggatcc attgaatcgg
     4561 aatcgtgaac acttcattat ttgctttgaa tgaagcagaa accatccata aaaaaggaat
     4621 gatcgtcaat aaagctaata caatcagcag aatcataccc aaccattgaa taaagggcat
     4681 gttttttgaa cgaatttttg ctttttttct ttcatttcga acacctccaa agttataatc
     4741 ttcatttctt tgtaatgatt acaatccaga acaacggtta ttatagctga ttcatttccg
     4801 ttcttcttat ttttgtaagc gcttatttaa caagttatgt aaatctcttt atttttttga
     4861 tttaaaaatg aaaaaataca aaagcaagac tgtcatacgt ctcgcttttg tattgaaatt
     4921 tcttcgatta gagatactta ttactgttca aaagcttctt tgaaacggtg aatcaattca
     4981 ttgactacag gtgataaaat ccgattcttt ttccagacta gcatgcagtt cgtttcaatc
     5041 attgggtcaa gcggaacgaa tgtcactcgc tttgaatgat gacttacagc accttctatg
     5101 actaaagcag cacctacctg attctcaatt agcgcaaata cattgaaaat caaattgtac
     5161 gttcctgtga tcagcaactg ctcctttttt atgcctaacc agtcagcaaa cattcgctgt
     5221 atttctggac gctttgagat caaaagaggt acattcgcaa agtcttcttt tgttatcacg
     5281 tctttttttg ctaaaacaga attttctaaa atgagcaatc cccatttttc ctttcttggt
     5341 aacaaaatcg tttcgtattt ctctgtatct actggatcta gcaaaatcgc taaatctaac
     5401 aggctttttt ctagtcactc cgtgatatca tcacttgttc cgctgtggat atgaaattga
     5461 acgctgggat aatcacggat caacttttct agtaatagtg ccaatgtatt cgaactatca
     5521 gcttccacac agccgatcgt aaatcggccg ctgaataatt gtttttttct gttctcgaat
     5581 tcttgagtgg tttgattcgt taaatctaaa atttcttctg ctcggctttt ttagaaaata
     5641 acctgcttct gtcaagatca ttttgcggtt ttcgcgtaca aacagttctg ttcctagtta
     5701 ctcttctaac tccttcagtt gtcggcttaa ggttggttgt gtaagatgta gaacttctgc
     5761 ggcttgagag attgttcctt cttctgcaat tgtccaaaaa tattttagta agcgtaactc
     5821 catagcgatg ctccttgatt tttttacttg atacattcca ttttatttct agataacaca
     5881 cgatcatatt tcttgatttt tccttgtaaa cgttccaaac attcttgcat ttcagcaatt
     5941 tttttcgtca aaagttcact ttcttcaatc aaaagcttac gccgctcaaa tacagttgct
     6001 tctccttcgc tagataggcg aatatattca atcaaagact cgatcgataa tccggcattt
     6061 cgcatacaga tcgtaaagtc gacccatttt aggtcttctt ctgtataatc acggattccg
     6121 ccttttgtcc gattaactgg tggaatcaac ccgattcgtt cataatagcg caatgtatcg
     6181 gccgaaatgc ccttttcttc actgacttgt ttgatattca tctgatcact ctttctctta
     6241 atttcttgcc actttactta tgtattttat ttattctaca actttaagtg tactccaagt
     6301 caatactcat ttctattaaa actaagctgg attatctata tcggttgttt gtgtattatt
     6361 tcaaataaaa aagtcttgtt tttttattct tttttggata tacttttaac aatttaggga
     6421 aattttagga aaaggggtat aaaaatgaca acgtatcagc gaaaggtatt agtatcgaca
     6481 tcggcgggaa ttgctttaga gaatatggat attatgtttt tagctttttc attatcttca
     6541 atgattactt cttttcatat tagtggtacg caagcgggtt tcatttcaac gatcactaat
     6601 ttggggatgc tcattggagg tatttttttt ggcatcatag ctgataaata tggacgtgtg
     6661 aaagtatttt ctcaaacggt ccttctgttt tcgattgctt ctttattgat gtattttgct
     6721 tcaaacatct acttggttta tctctttcgc tttatcgcag gtgtaggagc gggaggagaa
     6781 tatggggcgt gtatgtcctt gatttccgaa agtttttcaa aaaaacaaat tggacgagct
     6841 tcttcggttg ctggtattgg tgcgcaggtc ggtgcggctt tggctgcgat tttagctgct
     6901 gtgatcattc caacgtttgg ttggaagatg ttgtatgtcg taggtgtact gccggtgttg
     6961 atggtcttgt tcattcgtcg cggcttgact gaaccaaagg aattcaaatc tgtacaatct
     7021 gccgaaaaga aaaccaaact tagcagactg tttgaatcaa aacatcttgc ttggcaaaca
     7081 tgtggcctta gtatcatggt aactgtccaa attgccggct attttgggtt aatgaattgg
     7141 ctaccttcaa ttatgcaaaa tcgattaggg ctttcggttt ctgggtcttc attgtggatg
     7201 gtcagtacta ttttaggcat gtctttgggg atgatgactt ttggcataat catggataaa
     7261 ttggggccta aactagcctt ttctttgttt ttaatttgtt catccttatc tgtctatcta
     7321 cttgtgttag cccatagcca gtggagtttg gtagcagccg ctgtgatagt cggctacttt
     7381 atcaatggca tgtatggtgg atatggcgca gtgattagta gtttgtatcc aacagaaatc
     7441 cgcgcgacgg cgaataattt cgttatgaac cttggacgag ctgttggtgg tttttcttct
     7501 atcgtcatcg gtttcttgat ggatcattat agtttaactg ccgtaatctt gtttttgagc
     7561 ggtatttacc tagtcagtct gctggtcttg acaactctta ctggtgttac agcattaagg
     7621 gaaagcttgc agcattgata gttgagtctg taaatgacaa gaaagaggtg tggaaaaagc
     7681 ttgcatctgc ggtaataaga ccagccaaat caaaaacatg agacactgtt tccgtttggc
     7741 tggtcgtatt acttgatgca gggcagcttt ttcaactgct aatttccttc tatctggcgg
     7801 cgactattct ttgacgtccg tcatttcaaa gtactttact aataacttag cataacgaaa
     7861 aactgtggat acactcgtat attgagcgtc tgcataatct tccaaatttt tctcaggatg
     7921 ttgatacatg aattcaatca aatgataatt atatgatttt ctgtagaaat gaagataaat
     7981 aatgtagatt gggaagttct cctgcttttc catagaaaag cgccgattat tctcattctt
     8041 caaaatgacc ttatcaggat attgttggaa caattcttct aaatctgtta gataattcaa
     8101 gatagactta cttgatacct tcaattcgtt ttgtagttct tctattgaac accaacgttc
     8161 tgtttcaagc agtaaatgaa tcattttaat tttcttttga gaaacttttg ttaaaaaagt
     8221 ctgcatattt tctcctcctg tatctacttt actatactac atacaagaaa aaaacggata
     8281 caaatatcat attgtcacac cgtcaatagt gcataaacct gttattattt ctcttttttt
     8341 cctaagtatt ctattgatat gaatcattta atcgtttatt atataaatgt caataatcta
     8401 ataataaaat aacgtacata ttttattatt gcatacttaa taacagaact atagcaaaaa
     8461 atcccgtgta tcaacacggg attagattaa gtttcctcaa tcactattca taacacaatt
     8521 attataacaa gtaagtaaac aattgaaaag gacatttatt gaaaacggtt gttttttact
     8581 taaggaggaa atttagtgaa aaaattagtt gcgttgtttt tggtcgcagg aagtttgttg
     8641 agtgggtgtg gtgctactac gtctactact caaagcacaa gttcagctca ggcttccagc
     8701 agcagtacca cgatctccga aagtacggca gattcatcga aagaacaaat tgaaaagaaa
     8761 aaagaagaag agaaacaaaa agcggaaaaa gctgcactag aaaaaaaagt gacagaagcg
     8821 gaatctgctt tgtcacaagc tgaagcaaat cctacaaaag aaaactatgc aactgcttca
     8881 gcagctatcc aagcgctacc tgaaaacaaa caagagttga caagccgtct tgctgcaatt
     8941 gattccacaa tcaaagcaaa agaagcagaa gaagaacgga aaaagcaaga agaaattgca
     9001 cgacaacaag cagaacaagc gcaacaagaa gcaatcgcac agcaacaagc agaacaggcg
     9061 aggcaagaag caatcgcaca gcaacaagca gaacaggcgc gacagcaagc agcagctgaa
     9121 caggcttctc agcaggcaca agccgcagct cctgagcaaa ataatgaaca aactgtctat
     9181 gtcacaccga ccggttcaaa ataccataca cataaatgtg gaaatggtac gtatacccct
     9241 gccactttaa gcgaagctca agctcgtggt ttaacccctt gctccaaatg ttatggaaac
     9301 taattcaaaa gttatactat aataataaaa ttcttctgaa acataaaaaa atagaagctg
     9361 gcgttccaga tagtaaacca aaaatagcag tttacacgtc catttcgtca gcttctctct
     9421 tttttcattt agtctgttcc ctctgctttt ttggacatct tcattcattc tttctcaatc
     9481 aaatagtaca cctctttttc ttttcgcagt ttatatcttc cttttttcaa tttcattgaa
     9541 aactgtttcg aatgtttatg ttttacatgg attttaagca ggagctcctc atgttgcaag
     9601 cgatagttcc tgccttttaa atcagaaaat ctcactagtc cgtattcatc agaagctttc
     9661 acaaatggaa ctttttctct agtaatatag tgtttgccgt tgagcgtttt tacagaaaag
     9721 accattcctt cgattggtcg tccgtttgtg ttctttacat aaaaagataa aggatagttt
     9781 cgcttagcga tggccaaaga atctaaataa gcgatgagta gccaaactaa gccgattgat
     9841 ccaagtagta taccgataaa ccaaataaaa aatttccatt ttttccattg atcaattcct
     9901 tggatcgctt tttttgcctc ttttgcttga taaggaatac gatgccccgt cacaagcagt
     9961 cgatgtgtat tgaccatata aggagtgcag gtaagcaaag tcacgagatc tctgcctttt
    10021 tggataccca aagaatctac ttcgtcaggt aagaccactt ggattttttc tacttgatac
    10081 gccaatgtct ttccattgat ctggatgaaa aactggtctc cttttttcaa cttaggcaaa
    10141 tctgtaaata attttgcttc tgggagtcca cgatgcccag aaagaacggc atgggtgttt
    10201 ttcccgccag tcggataaga acttccttcc aataatgacg ttcctttttg caaaaatatt
    10261 tccgttgtct gatcgaatat aggtaagctg acattgattt ttgggatttc gatgactcca
    10321 agcatatgtt ccataaaaaa ggcattacta ggtaaatctt ttaatgcttt tgcatcaact
    10381 gcttggttga aactgcctat ccctggtgat acgttttgct ctgccaactg ttggtttttc
    10441 tttgtcattt tttcctgctg tttttttatt tcctctgaat tcttattcga tgcttgcttt
    10501 tgataatgga tgatcatttg ctgggcaaga aaatcattca gtgaatcttc tacaaaagga
    10561 taagaaaaga tcccgatccc aacgactaaa atgattgcca tgaagatgtc cagtattctt
    10621 attctgcttt tattcatcat gtccacctat tttctaatat gtatatgaag aggttgtgaa
    10681 atcagcgcct tgacctgagt attagcgaag caaacggcaa aatagttcct cctattttgc
    10741 cgtttgttga gcgaatacca agagaccgac cacttgtcac aacctttctc cccatttgaa
    10801 tcactatcgt ttatttatcg tatgattttt gtttccaata gtttaagctt ctgtttcttg
    10861 atggcgtttt ttgaaataga tcactgctcc tccaactaaa gcagtaccta ccaagatgaa
    10921 tacgatgatc cccataccac cagttgaagg caagattcct ttaggttggt tatcgatcgt
    10981 tgtttcatca gaagttgttg gatcatcatc tactgtaaat tcagtgtacg cagcttcatt
    11041 cttaggtaat gcgtagcctt ctggtgcttt tgtttcttga aggcagtatg ccccttgttt
    11101 cagcccttcg attgaaaatt tcccttctga atcagaaatc aatttcactt cttcccagtc
    11161 agaacctgtg acttcatctg accaagcata tgggtattct cctgaggcaa ggtttgctat
    11221 ttttttcatt aatttaacag ttggatcttt tgctcttaga atagaaaatt ctgcatcggc
    11281 taatttttct tgcgtgttca catcttgttt gatgaagtgg aaatcaccgt agttttggac
    11341 attttctaca gtaatcgtgt tatccgtagt tgtattttcc cgattgacta caccgtttgg
    11401 attcccaaga ttggcttcat ctgcgccatg gatgataccg gttgtttgtt gcaattttcc
    11461 gttttttact ataaaagtag tttctttttg tggattggtt aattgataac catctggtgc
    11521 ttttgtttca actaaagtat aattgccgtc tattaatcca ttgacactga tggatccttc
    11581 agtatcagtt gtgaatgtca acgctgactc agcaggatca tagagagcga caccagtgtt
    11641 ttcatcaaaa ccggtcacga attgattctc tgaatttttg atctggaatt gcgcccctgc
    11701 taacttatct acccctgaaa agttcccagc tttctggatt ttgatattgc tttctttgat
    11761 gacattttta gcatataggt gaatcgtgtc taaaggtttg tcagggtcta gggtaagctt
    11821 aacacgatat ttggcgacgt ttcgacaccg ggctttggtg tttctacaaa gacgtataca
    11881 gcatctttcc catcagattt acctggaaga gtcaggttag ctaatccttc accagaagtc
    11941 gttacacttg cgaagggtgt agcgtctgct ggaactgctg tgtctttact tggatagtac
    12001 gtagcagtga tgtcatatac gttgaattca acgccgttca acggttcgct attcggaaat
    12061 gtcaattccg ttccgttgtt tatttgattt tctggtgctg aagtgaattt caatttatgc
    12121 aaattaaccg taacattttc tggtgtaggt gatacggctg cagaaacctc tgcagttcct
    12181 aggccgagaa cgaaaggaag cagcatgata gcagttccta aaaacgtcca tatttttgtt
    12241 ttgatgtttt tcattttatt tccctcctat tgtttccttt ttgttgtttt ttataaaacc
    12301 aagctgtccc tgttgaaagg actagcaata accctaaaat tgagaaacct ccaagcatct
    12361 cttcgcttgt ttttggaaag cttcctccat ttttttcatt tgatggacca tttgtctgca
    12421 tctgaactgg cgtttttcca ttattcgtcg gatttgcttt tggttcgttg atgatttcca
    12481 atggtatagg atgaagcgga tcataactgc ctttttcaac ttcaaagaga atggttgatt
    12541 gactcaatat atatccttta ggcggtttaa tctccactag ttcgtaactt ccaaaagaca
    12601 atccatggac agaaaatgtt ccatctttac tggaagtaaa aatcgttgct cttgaatcat
    12661 ctttggctac ccaatgattt atcccgtttt cttgcgctaa gtattggtca ttttttttga
    12721 ctagaaattg agcatctgct agtttttgtt tcgtttgccg atccactttg acaaattgtt
    12781 tgcctcctgt ttggatggca agatgtgttt ctactgtttg attgtcagtc gtcaaacgta
    12841 catcgttatc aaatgtcgtt ttttcactgt tttctttcaa tgtcatttgg taacggatcg
    12901 ttaatttttt tccagcaaac gaactaagat cttttccaga atctgataag acaaaaccat
    12961 gttttatttt ttgtgtgtga aatgtatgaa tcttgtctcc accaatcaaa aaatcgatcg
    13021 tttctggttc tagccataac tgaggatcag ctgtatcttt gatcgtatag gttcctatct
    13081 cgttgatgtt ctcaggaatt gttacggtga tgagataagg gactttatcc ccatctgcaa
    13141 aatctgtctg cttattctct actgtttttg ttaatgtgag tgcagcaggt attttttgtt
    13201 cgctctttgg atacaagtgg atggtcgtca atttatgatt agatgaatct gtcacaggaa
    13261 gcacaacgac aaatggtgct gatttttctt ttacttaatc tgaagtcttt atttccgtaa
    13321 atcggtaaac agcgtcacga ccttgctcat ctttgtccga tgcactaaaa gaagcaatgc
    13381 cctcttcctc gtctactgtt tttgtcacat tttctgccag tattttttca ctatcacttt
    13441 tttgtgctag ttttctttgt gcgtcttcca cactacttcc ttctgaccgc agcttgtaaa
    13501 attcatccga gacatcatat atagcgaaag taacgccgtt caaccctttt actccttgaa
    13561 agttatccgg gatatttatt ccgtcgtttt gactgatact tggcatctta tcgctttcga
    13621 acattcgatt atgcaaagta aatgtaatag gtgattctct agctgacaca gtcggagaac
    13681 ctataaaaaa gaccagtagg ataaaaaaga gaacagcaaa tatatccaca cgtacatttt
    13741 ttgttttttt catcgctgtt cacctgcttt tttcttacga tggataaaat aaaagaagcc
    13801 agaaaaaata aagatcagtc ctaaaaagac aaagggcagt agacctatcc cacctgtcga
    13861 aggaagcaag agagtcggat tgatcgtcaa cttagcggta tctgttgcga taatcgtata
    13921 attattgttt acacttcctt cgcctaaact aatcgttact atttgtgatc caatatctgt
    13981 ccggctgtta actgttaggg tataatccaa tgattcgcct aagacaacac cggtaacgtg
    14041 gagatttcca accgaaatcg agtgactttg ggcatcataa ttaaaggcag cgtcatcgac
    14101 agtaatcact gcgttagctt tatagatggt ataagttcct gatgtgttgg ctatatcagt
    14161 aaatgacaac ttgggatttg ctgctttgac tttgtctatt ccgctattcg ttagtttaat
    14221 tgtatagctg ccagcattaa ccatattttt tgctgattgt gcatctgctg tgtaatccat
    14281 actgctaagt gccacttgta ctggagtaag cgaactagtg acaccctctg ctagcttgac
    14341 atggattgac gggacacctg cttttccatc atatgtctta ctgccactga gctctaccgc
    14401 accgctgatt tgcttaatcg tatagacatc agatgtctgc acgatagtaa ataaatagtt
    14461 tgttctggca gctagtttag ctagtccgct ttttgtcaat tgccaagacg tataggttcc
    14521 aatttcagta ggcgcagtta cttcaactcc cttttcattc atcgctttga aatcagttgg
    14581 attccatgtg atttgatcaa tactgagatc attgtttaaa agggatgttg attctgtttt
    14641 atcattaaag gcatatttaa cgatcggtac aacagaaaga ccatcatagt acttactgcc
    14701 atttgcattg attgatactg ttgcatttgt cttataatat aggatgactt cagaaatctg
    14761 ttttccattt gcgtccttta cgattacatt tccatttgta tctaaactag ctaaaacact
    14821 cattgctgat tggttctttg tttctatcgc tgaaacagct gtaaaggtat atccgttgat
    14881 agtaggcgta ttagtcgtca ggtcttgtgc agcacttccg taagtaagat tcattatttt
    14941 agaaggagca atcatctgtc caaaatagtc ttgatactta atagtgattg gtgtttgttt
    15001 gcctgtatag gtcaccgtat cgacgttact ttctagggct gtgaaagaac cgtttgtttg
    15061 tgcaaaagaa tagcccgtta ctgctgttgg agtgataacc actttgtcac ccacatggat
    15121 tccagataat gtagtcccgt acgttccgat ttttgctgct ccaccattga ctgtataaga
    15181 aaaagttcct gtccgattcg cattgacctg aaccgttgct gcttgatagg ttggaaaatt
    15241 tgaaacattt cccttaccat caacgatctg cccatttttg atcgagtacc ccgtgaatgg
    15301 aatatcattt gctaactgta taccgattga aaaagtactc atacctcctc cggcaggaag
    15361 ccctccccat gttgagggtg taaaagaata acttttatcc gcattgggtg taactgaatc
    15421 gatcgtattt ccagcggtag ccacccccat atagatactt tttgggaatg tgatagtagt
    15481 accatttact gtggagccaa taaacagatt atggatatct ggacggttat caataaaagc
    15541 gattgctttg gttacgcctt gtaacgcagt tttatccgca ctcatcgatc cgttaaccgc
    15601 aacaatgact ccatctttgg catatatcac tgtatatcca tctggtatgg tatgtccgtt
    15661 gaaattcgca gtggccgcaa cagtactaaa tgtctggctt ctacctgttt ctgaataagc
    15721 tttctcccct gctgcttttg ctgctgtgat ttctttttct gtcggatcaa tcagttgagt
    15781 cgaggatgtg gttgaggttg tctcgttgtt gctagactga ttggtgcttt ctatggactg
    15841 tatttcagaa tagggtggac tatttgtctc ctctgtcgtt tggttagcaa aatttgacgt
    15901 ttctgtataa acgtttacat aaatacttga taagctccct aagaaagtaa aaatcaagat
    15961 gatcgaaatc ataatgggat gtaagtattt tcttttagat acagctttga gtctagaaac
    16021 aaaatgtttt tgacatatac gttggatttt attttgagtt ttcttaatca ttttcatccc
    16081 tcctctcaat aaaaaatcat caaataatcc tttacacgtt attctaaat
//
LOCUS       DARBDV010000068        16022 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000068 DARBDV010000000
VERSION     DARBDV010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.068"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            226..894
                     /locus_tag="SCM56_002046"
     CDS             226..894
                     /locus_tag="SCM56_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEG1110800.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1480)
                     /locus_tag="SCM56_002047"
     CDS             complement(1094..1480)
                     /locus_tag="SCM56_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110801.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1452..2834)
                     /locus_tag="SCM56_002048"
     CDS             complement(1452..2834)
                     /locus_tag="SCM56_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEG1110802.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2990..4177)
                     /locus_tag="SCM56_002049"
     CDS             complement(2990..4177)
                     /locus_tag="SCM56_002049"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEG1110803.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4184..5215)
                     /locus_tag="SCM56_002050"
     CDS             complement(4184..5215)
                     /locus_tag="SCM56_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEG1110804.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5231..6685)
                     /locus_tag="SCM56_002051"
     CDS             complement(5231..6685)
                     /locus_tag="SCM56_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1110805.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            6860..7765
                     /locus_tag="SCM56_002052"
     CDS             6860..7765
                     /locus_tag="SCM56_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEG1110806.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7856..8533
                     /locus_tag="SCM56_002053"
     CDS             7856..8533
                     /locus_tag="SCM56_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEG1110807.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8530..8700
                     /locus_tag="SCM56_002054"
     CDS             8530..8700
                     /locus_tag="SCM56_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110808.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8814..9710
                     /locus_tag="SCM56_002055"
     CDS             8814..9710
                     /locus_tag="SCM56_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG1110809.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9765..11135)
                     /locus_tag="SCM56_002056"
     CDS             complement(9765..11135)
                     /locus_tag="SCM56_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1110810.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11373..12119
                     /locus_tag="SCM56_002057"
     CDS             11373..12119
                     /locus_tag="SCM56_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEG1110811.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12116..12955
                     /locus_tag="SCM56_002058"
     CDS             12116..12955
                     /locus_tag="SCM56_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEG1110812.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12956..13906
                     /locus_tag="SCM56_002059"
     CDS             12956..13906
                     /locus_tag="SCM56_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEG1110813.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14047..14799
                     /locus_tag="SCM56_002060"
     CDS             14047..14799
                     /locus_tag="SCM56_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110814.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14818..15969
                     /gene="queG"
                     /locus_tag="SCM56_002061"
     CDS             14818..15969
                     /gene="queG"
                     /locus_tag="SCM56_002061"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEG1110815.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5200 a         3005 c         3225 g         4592 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttctcttg aaaatgccaa acatgttatc ctagagaatg ctggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt ttgataccgt
     1381 tgactagttc ttccatcgca aaagcagtcc cccattttaa tccaaatggc cgatctcgat
     1441 actcgttata gtcaatgaat ttttttgctg tccgtctcat cttttccacc ttctaatcca
     1501 ctcataccac ctgcatgccc gccaacgaga gaactacgtg cgatggcgcg tcctccttgc
     1561 aagagactat gcgcatggat gattgattga aagccgtatt tttttctaat cgtatcgaca
     1621 acaaaatcaa ctttcgcttc atttatctgc ttttcaggat cttcaaaaag gttaagctgt
     1681 aaacctgtcg cgtatacgag gtcggaacag ttcacgccta cacgccgaat atcctggtag
     1741 tggtaatgct ggtggaaaat ggtcaataaa gcatctgcta gatctctgct agcatttgtc
     1801 gcttcgattt tcatttgctg gtgaaacccg gtcttgccat ttggatcaag atagcctaaa
     1861 gagtatccta cccataagct gactacttgt gtttttgctg cagatcgtct caagcgagtg
     1921 ccgacttggt cggccatttc tttgataacg atctcgattt cttctcgtct tgtataatct
     1981 ctatctagga tttgactatt tccgatactt tttgattttg gtgtatattc ttgtcccaaa
     2041 aaacttcgat cgatgcccca agcatgggca taaagctgag taccgatgac ccctagcttg
     2101 cttttcaagt gataataatc cgcgtgagct aaatcataaa cagaatgtat acccatttgt
     2161 ttcagacgtt ttgctgttcg tttgccgatt ccccaaaagt cagtgatatc tgagattttc
     2221 cataactttt ctggtacatc ttcataacgc cattcagcaa acatttgctt tccgtctttt
     2281 gagccattgt ctaaggctag tttagcaagt agcgggttat cgccaattcc tatagtcgtg
     2341 tagattccgg tctgctcaaa cacatcttta tggatcagtt tagccatgtc gtacgcattt
     2401 gttttaccaa agagtgccat gctatttgtt acatcaagga agctttcatc gacggaatag
     2461 acatgatggt tcgcttcgtc tgcatatttt ttatagatag aattgatttc tctgttcttt
     2521 tccatataat aggccatcct tggggcaaca atatataaat cacttggata ggggaaggga
     2581 agatctctgg cacggctgat attggtgatg ccgtaagctt tttttgctgc aggactggaa
     2641 gccaagatta atccgctacc ccgtgtttct gtcgaatcgg acggatagga catgactact
     2701 aatttagttg taaggggatc aagatccttt tcaacgcact ctacggaggc gtaaaaagat
     2761 ttgcagtcaa tacataaaat atctcttgaa ggttcttttt cataaacaaa taccggattt
     2821 tgattcgccc acataacaat cacctcttca acacaattag aacacacgtt ctctttccgt
     2881 gtcaataaaa aacagcaatt ttcaaatacg agcaaaaggc tcacatttgg aaattgctga
     2941 gaggttattt ttatctattt gtttttagct gagatcgttg atttttttat caaacatgaa
     3001 cagcttctgg atcgatcttt gttgtcccat ttccaatgtg tgcaacataa aattccggag
     3061 catagccgac aactttcatg tattcatcat agacattgtt tttgaaggca ggtatatttt
     3121 cttctttgac aagagcaatc gcacagccac caaatccagc tcctgtcatt cgtgcaccta
     3181 agacaccttc ttgtttttga gcagcatcta ctagtgtatc taattcgatc ccagttactt
     3241 cataatcata gcgaagagag tggtgagaat catttaacag ttgaccaaat gcttctaagt
     3301 tcccagcttc taattcagct tttgccttca aagtccgctg attttcagaa acggcatggc
     3361 gagctctgcg tatcaatgta tcatcgccga tttggtcaat agttgattca aattcttctt
     3421 catctaagtc tcctaaagaa gagataggca aagttttttg caaacgtgcc aaggcttctt
     3481 cgcattcgct acggcgttca ttgtattttg aatctgccaa ttcccgccgc ttattcgtat
     3541 tcatgatgac gattgcataa ccatctagtt tcactggaac catttcgtat ttcagcgtat
     3601 ttgtatctaa taagattgca tgatctagct caccgaagcc gatggcaaac tgatccatga
     3661 tacctgaatt gacaccgatg aattcatttt ctactttttt acctgtttgg actagttcca
     3721 agcgatctgt ttttaaatca aacagatctt ctaatacaac acctactaat aattctaatg
     3781 aagcgctact ggaaagtcct gcaccattag ggattgttcc ttccactaac aactcaaacc
     3841 catgatcgat tgagtgtcca gcttctttta atttaaggac cattcctttt acgtaatttg
     3901 cccagtttgc ttttttatca tattctagct catctaaagt aaaactaatg acaccgatat
     3961 cgtcaaagtt tgtggaataa actaatattt tattatcgtc tcgaggtgct gctacacctt
     4021 gcgttccata agtgatggaa gcggggaaga catggcctcc attgtaatct gtgtgttcac
     4081 caatcaaatt gatgcgtcct ggtgagaagt attgtgtagt ttttttaggt ccgaatgttt
     4141 cggcaaattt tgtgtttaag cttcttttga tttccatgat gagttattcc tttcttgttt
     4201 tgattttgaa ttctgtcact gtttcaaata ctgatccagc ttttaaatga atcgaaccaa
     4261 atgctgagaa gcgctctgca cctggagctg tttgagtctc aaatgtgatg ccaccgtgat
     4321 gtgccagttt tcggttgcgc atttctggtg tttctgtgcc gaaatttgca gtgaaaataa
     4381 ccacactaga agcatctgta gcaataccta cttggattct tttatcgggg ctagtcaagc
     4441 gagcggcttc tttacccaag ccggtttctt ttaagaaaaa tggatgatcg attccgtcaa
     4501 caagattttt ttgatccaaa tcactagcaa aaacggacgc taatttttta ggtatttgaa
     4561 aatcgaaagc acttcctgca gcattttctt ttacccctat aggaatacta tctgttcgca
     4621 aaggagcata agcttcgcta ttgagccaaa gctcatgttg atcgattgac tgagaagcat
     4681 ctcccgtcaa gttgaagtaa acatgatttg tcggattgaa taaagtatct tggtcactgg
     4741 tgccgcgaga ggtcactcgc cagatattat cttttgtcaa agtatagcgg atttccacat
     4801 ccattgtacc tggaaaacca tgttctccgt cgggacttgt agtagtaaaa atgacagaag
     4861 cttcattttc gccattcaaa atgacataag accatttttt taattcaaat ccaggagcac
     4921 caccatgcag attatgtcca gtttttggat ctgtggctaa ttggtaagtt tttccattga
     4981 gagaaaagcg gccattttcg attctccctg cggtgcgacc gatagaagca ccgatatacg
     5041 gatctttttc gatatattct tcagcggtgt caaatcccaa aactaattct cgatcatttg
     5101 attttaaact gacgattcta gcacctaaat cagagataga aagtgctagc ttgtttttgt
     5161 tctccaaggt tatcaagtga taaccttggc caaaatcttt ttctgtaatc ttcattttca
     5221 agatcctttc ttaatgggaa ggaactactg catcgtgaag ttcatcttcc attgtatcag
     5281 ctggagccaa tttgttttcc aattcctcaa cgatttgtgc atgttttttc tctgtcaatg
     5341 taatcttcca agcaaaaata atagcggaga aaatcatcaa agcagcagga gcatagaatg
     5401 cataagcttt gaaagtcgtt acgccatgag cagtgatatc agcagcagta gcattaccag
     5461 tcatcccaga agcgatagcg acaaacccta cgatcccgtt agacaaagca ccagcgattt
     5521 tatctagtaa agggcgaatt gatagagtaa cagcttcgtt acggatacca tttttccatt
     5581 gaccatattc aacagagtct gtgatcgtca tcaaagcgga taagaaaatc aattgttgag
     5641 ggaagtagaa caatactagc ccgatgataa cgattggcaa acttgaacct gcgatcgtaa
     5701 agaaaaagta agaagaaatc atcattgcga tccctccaag gtaaacgtag cgacgagaga
     5761 tgacgcgtgt aagcattggg aatagtggaa cagctactaa accaagtaag gcagcaacta
     5821 gaccaacgat ccaaaattca gttggttggc caagtacata tttgaagaag tagaataata
     5881 cggcagttgt tgctacattg gcaatcgcat aagcgatata agcaagggca agccacatca
     5941 actgatcatt ttgagcaact gctttgaaca catctttgat cccagctttt tcattttgct
     6001 gacggatcgc agtatcgact tcttttgtgc ccaaggcagt gataatcgca gtaccgcctg
     6061 aaataacagc agcaatcaca ccgaaccaga accagcctga agcgccttca atatgtttat
     6121 gagtaacaag ataggtaaag aaagtaacga tagggacaac gactagagta gtaccatttg
     6181 cacctagaga agagccgaaa cgagcaaacg tagcaaattt ttcacgttcc tttgtttccg
     6241 tactcaaagc tggaatcatt gaccaaaaag cgatatcctt aaatgaataa aaactatcca
     6301 ataagatgaa aacgattgca aataatatga taaataaagt cgtattatga gtagctaagc
     6361 caaagaaatt ggtaaagatt gctaccaaga gtacggaact aacaacgccg ccgataacta
     6421 accatggttt gaatttaccg tattttgtac gagtattatc gatgatgcca ccaatcaaag
     6481 gatcaaaaat aatttcaact aagcggatac ctacaactaa gcttgtaact gtagcaatca
     6541 tttttgcatt cgttgcctta tctgttcctg cgaacatctg gcttgtcacg aataccataa
     6601 aataagtgct taatgtaaca tagaatgtgt cgtgtccaaa agcgcctaaa gcgtaggaca
     6661 cacgttggat cattttgctt ttcataatct gtattcctct cctgttcaat atataatact
     6721 aagtaaaccg gttacaacga ataacaaaga tcttttaatg acttaaactt aacaagaaat
     6781 cgcttccttt ttaatatatt tctgtgactt ttatttaact ttttgttgca ttatactaaa
     6841 ggagtaagga gggatagacg tggagcaatc gattttttta aaaaaggaac atcaacaagt
     6901 agaaaaagaa ttgtattttg atttttgtgg tttctctaaa acgatgccgt ttcattcttt
     6961 tggtcctgct attcgcaatg attacatcct gcatgtcgtg atggaaggca aaggatctta
     7021 tcatgtgaaa aatcaacaat tccaattgaa aaaaggtgat ctgttcttga ttcgtccagg
     7081 agactcgacg ttttacttat cagatggaga agatccgtgg atgtattgct ggctttcatt
     7141 tggtgggagt gctgcagaag aaatcattcg gcagtcgctt ttcagagaag accaatacac
     7201 gatggtctct tctgacatac aaaaatatat cgacattatt ttagaatgca tgaaagatac
     7261 ggaagaaacc ttggaaaatg aattacagtt aacagcatta acctaccgtt ttttatcagt
     7321 ccttttaaaa gatggcggaa aggtcagcgg tggtgtacaa aaggttcatt cctctttagc
     7381 tgttgaaaca gtcaaataca tcgaggcaca ttatgctgaa aaattgtctg ttgaggaaat
     7441 cgcccagcat ttagcggtca atcggagcca tttgtcgcga gtattcaaaa atcatatggg
     7501 taccagtatc aaggaatatt tgatcggggt acggatcaat cgaggggcgt ttcttctttc
     7561 tctgacggat gaatccgtgg aaagtattgc ctatcaagta gggttcaata gtctagtcgt
     7621 atttagccga atgttcaaaa agagtaccgg agaaacggct acgaactatc gtaaacgtat
     7681 gaaaaacgaa aattttaaga atattgcttt ggataccttg aaaaaacagt tggaaaaaca
     7741 aaccgcagtt tcttggacaa cctgattagt ctggttaaaa tgacaagcat cggcaaaaga
     7801 aacttgtggc ggttatgcta cactgtaaag cgaagactaa aaaggaggaa aagggatgcg
     7861 tacacttatc atcattagcc atccaacgat cgaagaatct ttaaatcagc aatttttcaa
     7921 agaagcttct gctggtttac cagctacttg gcatcatttg gaatcaagtt atccagacgg
     7981 aaaaatagat atagagactg aactacagct cttggcccag catgatcgga tcattttcca
     8041 gtttccgttt tactggtata gttctcctgc tcatttaaaa atttggcaag ataatgtctt
     8101 ggaacgagca gcacatgtat taaaagggaa agaactagga atcgtattga caacaggtgt
     8161 ggcggaaaaa gaataccaag ctggaggaaa agaagaatat acgatttccg aatttttgag
     8221 gccgtatcag cgaatcgcaa ataaattcca tatgacctat cttccgccat tcgttttagc
     8281 acaatttatg tatttatccc aggaaaaacg ttgggagaag ctaattgctt atcagcagta
     8341 tctctcattg gaaggcaaac catcgctgac tcagcggatt gactggttta ttcaaagagt
     8401 acaggaaaat cagaaaatgc aggaagaaga atctgaaaaa caagcatata tcgtagaagc
     8461 actgactgag gcaaaagagc agatcgaaga cttgaccttt acactgcaag aaatgaaagg
     8521 aacttcatta tgacaaatga ttggctgatc caacaattag atgaattaag cgaaaaatca
     8581 gaatatcaag accgtgcgct cttatatgaa ataaagaaac tgatggctga gatagacgaa
     8641 cgaacaatgc agctgcaagg agaaatcgac ggacaatctt ggaatcatct caactggtga
     8701 aattttctta taaataacaa agataaaaat gtgcacagcg acaattattt gctacaataa
     8761 gaatatgaag cgtttgataa cgctattata cataaaaatt ggtggtgaaa aaaatgtatg
     8821 atgaaaatga gtggggagaa aatgttcgga tgacccgaag aagacagcaa aaacaggcag
     8881 ctcgtaagcg gatgatcatg attggggcag cagttgccgt gtttttgatc atagcaggag
     8941 gagttgcggg tctatatttt catggaaaaa attcgtcaga agcgaaaagt acgaccataa
     9001 cggaaacaac gacaaagaaa acagagacga aacaaacaag tgagtcatca actaaaacaa
     9061 aagagacaca aacttctgaa gaaaaaacag caaatggttt gccagagcaa cttcaagcaa
     9121 tcgtaaatga ttattcgaaa aagatcgaag aaaagacgcc tgcattgatt gaagaatacc
     9181 agacagaaat tcagggaaat caagagggaa tagctggttt atcagcagtt gctaaccaaa
     9241 aagcacgtga gcttcaagca atctccgacg aagggatcag aaaattacgt gcagcgtatc
     9301 aggcagcaga aaataaagac ggtgtagact tagatacttt gatcaatcaa ttatcggcta
     9361 attatacgaa tcatgtcgct aagatttccg atatttattt gcagactagc gcctctcttc
     9421 aagcagagtc aacaagcaca caagacacaa catcttctga ttctgagaca gcagagtcgg
     9481 tagaagcttc tgaaatgcaa gctcgaacaa cacaagacac aacagattcc tctgaaacta
     9541 ataccaacag cgcgacttca caggctgcga caaccgtagt tttggaagga gaaggtccaa
     9601 atcaaatagc cgaacgaact ggcgtaccag tagaaacgat cttgtcgctg aatgggatga
     9661 gcatggagaa ttatttcctg actcccggtg agacgctaaa attgaattga taaaagcaat
     9721 aaaaaagaac gaagcttcca ctaaggtgaa gcttcgttct ttttttattt tccggttctt
     9781 ttttcgcgta caaagtaatg gatcgtcatg taaagcaaga aaacgagtcc aataatcaat
     9841 gagattctcg tttcaggatt gataaacatg aaaatcaata cgatagctaa aaaagcaagt
     9901 gtcacataat tcgtataagg agatagtggc attttaaaag gatgatcatg cattttatct
     9961 gcgtgcttct ttttgaagcg aatctcgctg atcaaaatca ctacccaagg aaccatacca
    10021 ggcaaaatac ttgagctgta tacgaaaacg aaaatatttt ctgactggtt gatgaacatt
    10081 ggtagaagat aatttaagat cagaccaatt aagattccac ctgagattgc tacgacagga
    10141 taaaaaggcg caccgtgacg agatagtttc agaaattttt tagacatctt tccatttatg
    10201 gcaagtgtat agaccatacg acttgcgctg aaaatccctg aattgcaacc tgataatgcc
    10261 gctgtaatca cgacaaaatt aatcagtcct gcagcaaatg taatgccgac tttagaaaaa
    10321 gtttgtacaa aaggtgaacc aatctcactc aattgattcc acggatagat actcacaatg
    10381 ataaaaatag caccaatata aaaaatcaag attcgaccga tagtggactg gacagcttca
    10441 atgatcgtct ttttaggatt ctctgcttca ccagcagtca ttccgatcag ttcgattcct
    10501 tgataggaag caacaacgat cgataatgca aagaaaaatc ctttgacgcc tccagtgaag
    10561 aatccaccat ttttccaaag atttgaaatc cctactgcct gaccatgatt accaaatcca
    10621 aataagatta ccccaagacc tgcaatgatc atcaaaatga tcgttatgac tttgatgatc
    10681 gaaaaccaaa attctaattc gccaaacgcc ttgacagaaa taagatttgc cagcataaga
    10741 aacaagacga caatgattcc gggaataatt gccggcaaat caggccacca ataattcata
    10801 taagatccta cagcaatcac ttcacttatt cctacgacaa tatattgaaa aatattactc
    10861 caagcggtca agtagccagc cacaggatgg atgtactcgc tcgcgtaatt tgcaaaagaa
    10921 ccagtcgaag gatcaacgta aagcatttcg cctaaagcac gcataatcaa atacaaaaag
    10981 aatcctgcaa tcgcataggc tcccatcact gagggccctg tccaacgtat ggttgaggag
    11041 gcacccataa acagaccgac accgatcgtt ccacctagtg cgatcatttg gatatgacgg
    11101 gcactaagac ttcgagttaa cttttcttct tccatacaaa aactcctttc gttgttgagt
    11161 caacaagaaa aaataatcaa acgtttcaac acatgttttt cattattagg ttagccttat
    11221 tgaactcttg atgtgattct acagaaaatt ttcataaaat gcgaggaaaa ttcgaaaaaa
    11281 actttttcat cattctactt gaaaatgctt tccattttag ttaaactata tttaaataaa
    11341 aagttaggta aacctaaaaa ggagtgtaag atttggaacg tacggccatt gtaattcaag
    11401 acctttcagc cgcttatcaa gggaaaactg ttttgaaaac tatcaacttg acgataaatc
    11461 cacaaaaaat taccggtatt atcggaccaa acggtgcagg aaaatcaaca tttatgaaat
    11521 ctttgctaga gttgatacca gctactactg gtaaagtcac cttttctgga cagccggtaa
    11581 actcagtccg taaaaaaata gcctacgtgg aacagcgaag tgaattggat ctttcctttc
    11641 caattgatgt attaggcgtt gtacttttag gaacatatcc atctttacga attggacaaa
    11701 gacctgggaa aaaagaaaaa gaacgtgcaa gacaagcttt gaaaaaagta gggatggaag
    11761 aatatgccaa aagacagatc agcgaactat cgggtggaca gcttcagaga gtttttattg
    11821 caagagctct agcccaagga gcagaatgga tctttttaga tgaaccattc gtagggattg
    11881 atgcgttaag tgaacgaaag atctttgaca tcttgcagga attgaagaat tcaggaaaaa
    11941 cgattttgat cgtccatcat gatcttcata aagtagaaga atatttcgat gaggttattc
    12001 ttttaaataa acagctgatc gcttccggtc cagtacaaga agtctttaca tcgaagaacc
    12061 ttcaattggc ctatggtgag atcatcagac atttagtaaa aggaggagag aaaaaatgat
    12121 ccaatccttt attgatggat taatgaatta tcaattcctg caatacgctt tggtcacttc
    12181 catgcttgta ggacttgctt ctggagtcat cggttctttt attattttac gagggatgtc
    12241 tttgatggga gacgctatct ctcacgcagt tcttccaggt gtagccattt cttatatgtt
    12301 tggttttagt tacatctttg gagccactgc ttttgggatg ctaacagcag cagtgattgg
    12361 tttcgtgact cagcggagcc gattgaaaaa tgatacagcg attggaattg tctttagctc
    12421 cttttttgca ttagggatta tcttgatttc atatgcccag agtgcgacag atttgtatca
    12481 tattttattt gggaatgtct tagctgttag ggaaagcgat ttgttgctga cagcactcgt
    12541 tagtggaatc gtcttgatat ttgtcttttt cttttataaa gaattgaaga tcacatcatt
    12601 cgatccgaca atggcaaaag cttatgggct gaacacatcg ctgatccatt atcttttgat
    12661 gttctttttg acattagtgg ctgtagtcag tttacagaca gtaggaacaa tcttggtgat
    12721 tgccatgttg atcacaccag ccgccacggc ttacttgcta acgaaccatt tactgaaaat
    12781 gatcattaca gctgcaggaa tcggtatgct aagtgcagtt gtcggtgtgt ttttcagtta
    12841 tagttacaat tggccatcag gagctacgat cgtgttagca tgtaccgcat tttttatcct
    12901 tgctttttta ttttctccaa caaaaggaat tttatttcag aaaggaagca gatagatgaa
    12961 aacaaaaaaa tcactctttt taatcttggc agttagtttc ttggtattag ccggttgcgg
    13021 aaagcaggct tctgaccaag cggatgaagg aagtaaagaa aagttgtcag tcgtggctac
    13081 caattcgatc ttggcggaca tggcaaaaga ggtcggaaca gatcaaatag atatccacag
    13141 tatcgtacca gtcggaacag atccgcatga atatgaagta ctaccagaag acatcaaaaa
    13201 ggcaagcgat gcagatgtta ttttatacaa cggtttgaat cttgaaacag gtaacagctg
    13261 gttcgataac ttgatggaaa cggctaaaaa agaagaagga aaagattatt ttgcagttag
    13321 caaaaatgta gaacctcttt atttaactag cggtgaagaa catacaaaag cagatcccca
    13381 tgcttggcta gacctatcta acggaataaa atatgtggag gaaatcgcac gtatattctc
    13441 tgaaaaagat gcagaaaatg cgacactcta taaaaaaaat gcagaagcat atgtggaaaa
    13501 actaaaagaa ttagatacac aagcgaagga aagttttgct tctatcgaag agaacaaaaa
    13561 attattagta acaagtgaag gggcattcaa gtatttttca cgagcatatg atttgccagc
    13621 agcttatata tgggagatca atacagaaag tcaaggtacg cctgatcaaa tgaaagcgat
    13681 cattgatcag ataagagcaa aagaagtacc ggttttattc gtggaaacca gtgtagactc
    13741 aagaagcatg gaacgggtag caaaagaaac gggtttgaaa atctatgata aactgttcac
    13801 tgattccata gcaaaagaag gagaacaagg agattcttac tatcagatga tgaaatggaa
    13861 tattgaaacg atccatgaag gtttgtcaca aacaaaagaa agctgaaaag tgcaaggtct
    13921 agcttgagga gcagaccttg cattttttat aagagatatg aaaaaaaatt atgctgtttt
    13981 tcgtcgtaca gatacctttt ttgaagaaat tccgttatag ttagagtaag gaaggaaggt
    14041 gtggctatga attttacacg taaagataaa tatacgatta aagaagaagt ctttcgattg
    14101 atcagccagc aggaaaatac cgacaaggat caagaccttc aatttcttga agcagaatta
    14161 gctaaattat tcatagcaaa tcaaaatgag acaacagaac cagatgaaga gctgtacata
    14221 ggcaaatggt tgtttgatga atttcagcct ttagatgaaa ataccggtat cggttatttt
    14281 gtgttccttt ctccagtcgg cttcacacct ggcgtattag ttaaacaaaa cgacgaaata
    14341 accgtcaaag catggcctgc attattttca gtcatcaaag cagaaggagt aatcaaaaag
    14401 atccagtcgg cctattataa aagtcaattt attgccaaca gcaaattaat gaatatgttt
    14461 tataatcgtc tagtagaaat tgctaggcct gggaaaatcc cgcaaacatt cacgtacaca
    14521 cagcaaaaaa atctctatga agaagtattg tattcccgcg atctgtctga gttttcgcca
    14581 tgggaagagg taaggctcca tgaactgtta agtacaaaac cagtttcatt gaattttttt
    14641 aatgcattag ataacaaaag aaaattaatt tcaacaaatt attgggaagt atttgctgca
    14701 agaaaaaaat ggatggaaga aaagaagatt gccgatcagc agatccagtt tgtccagctg
    14761 aaaaaagaaa aactatatta tgggttatac agccgttaac agcaagcgag gaagaccatg
    14821 acattaaaag aagaaatcat acaagaaagc aaacgattag gaattgacaa aatcgggttc
    14881 accacggcgg aaccttttga ttcattaaaa gaatcgttgg aagaacaaaa agcagctggc
    14941 catacttctg gctttgaaca tccaaatatc gatgagcgtc tttatccaga aatgacattt
    15001 gatcagccga aatcgatcat ttccattgca cttgcgtatc ctactaggat ccatgaagaa
    15061 gtgcctaggg atgaaaaacg cgggcagttt gctcgtgctt cttgggggat cgattaccat
    15121 gatatactca gagaccgttt gaaccgattg atcgaattca tccaaacaag agcagaaaaa
    15181 tggcaaaaag aagaggaatg gcgtctagct ccgcaagtag atactggaga actgatcgat
    15241 gtcgctgtag cacagcgagc tggtttagga tttattggac gaaacggatt gttgatcact
    15301 gaagagttcg gttcatttgt ctatcttggt gagatcgtta ctaatattgt atttgagcct
    15361 gataaaccag gagaatttgg ttgcggtgac tgtatgcgct gcgtgactgc ttgtccgaca
    15421 aaagcattgc ttggtgacgg aagaatgaat gcccagcgat gtttgtctta tcaaactcag
    15481 acaaaaggga tgatgccgga agaatatcgt aaaaaaatgc gaaatgtcat ttatggatgt
    15541 gatatctgcc agttagtctg cccttacaat caaggaaaag attttcattt tcatgaagag
    15601 atggaaccaa aagtagacga agtatatcca aaattaaagc caatgctttc gatgtctaat
    15661 aaagacttca aacagcaatt tgggcattta gctggttcat ggcgtgggaa gaaacctttg
    15721 cagagaaacg cattgattgc tttagctaat ctaggtgatc gaacggcatt gcctgagatt
    15781 tcagaatgtt taacagatgt ccgccctgtc atcagaggaa cagcagcttg ggctattgga
    15841 cgtctaggaa caaaagaacc ggaaaaatgg cttgatgtat tagatgaaat attagctaaa
    15901 gaaacagaag aagtcgttat aaacgaattg gaacatgccc agaaattatt gcggagaaag
    15961 ttaaaataaa aaaatagtta acattattca aagtttttgt ccagtaattg ttgtaacaat
    16021 ta
//
LOCUS       DARBDV010000069        15949 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000069 DARBDV010000000
VERSION     DARBDV010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.069"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(386..808)
                     /locus_tag="SCM56_002062"
     CDS             complement(386..808)
                     /locus_tag="SCM56_002062"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEG1110816.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            complement(822..2228)
                     /gene="atpD"
                     /locus_tag="SCM56_002063"
     CDS             complement(822..2228)
                     /gene="atpD"
                     /locus_tag="SCM56_002063"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEG1110817.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            complement(2251..3153)
                     /locus_tag="SCM56_002064"
     CDS             complement(2251..3153)
                     /locus_tag="SCM56_002064"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEG1110818.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            complement(3167..4723)
                     /locus_tag="SCM56_002065"
     CDS             complement(3167..4723)
                     /locus_tag="SCM56_002065"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEG1110819.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            complement(4746..5288)
                     /locus_tag="SCM56_002066"
     CDS             complement(4746..5288)
                     /locus_tag="SCM56_002066"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEG1110820.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            complement(5275..5799)
                     /gene="atpF"
                     /locus_tag="SCM56_002067"
     CDS             complement(5275..5799)
                     /gene="atpF"
                     /locus_tag="SCM56_002067"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEG1110821.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            complement(5890..6105)
                     /gene="atpE"
                     /locus_tag="SCM56_002068"
     CDS             complement(5890..6105)
                     /gene="atpE"
                     /locus_tag="SCM56_002068"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEG1110822.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            complement(6158..6877)
                     /gene="atpB"
                     /locus_tag="SCM56_002069"
     CDS             complement(6158..6877)
                     /gene="atpB"
                     /locus_tag="SCM56_002069"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEG1110823.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            complement(7179..10022)
                     /gene="rnr"
                     /locus_tag="SCM56_002070"
                     /pseudo
     CDS             complement(7179..10022)
                     /gene="rnr"
                     /locus_tag="SCM56_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418328.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
     gene            complement(10025..10780)
                     /locus_tag="SCM56_002071"
     CDS             complement(10025..10780)
                     /locus_tag="SCM56_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEG1110824.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            complement(10883..11119)
                     /gene="secG"
                     /locus_tag="SCM56_002072"
     CDS             complement(10883..11119)
                     /gene="secG"
                     /locus_tag="SCM56_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEG1110825.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            complement(11237..11515)
                     /locus_tag="SCM56_002073"
     CDS             complement(11237..11515)
                     /locus_tag="SCM56_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEG1110826.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            complement(11628..12596)
                     /locus_tag="SCM56_002074"
     CDS             complement(11628..12596)
                     /locus_tag="SCM56_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG1110827.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     regulatory      12811..12855
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            12879..13853
                     /locus_tag="SCM56_002075"
     CDS             12879..13853
                     /locus_tag="SCM56_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEG1110828.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     gene            13858..14433
                     /locus_tag="SCM56_002076"
     CDS             13858..14433
                     /locus_tag="SCM56_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEG1110829.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(14500..15639)
                     /locus_tag="SCM56_002077"
     CDS             complement(14500..15639)
                     /locus_tag="SCM56_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HEG1110830.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT         4357 a         3442 c         2856 g         5294 t
ORIGIN      
        1 tgagccagaa tgtggttcat tctgactcag atttgatttt gcacaactca tcagtcctta
       61 ttgacaaaga gcccaaaagt cttgtcgcag tcccttattc caaataaacg gtgttcaatc
      121 atctgaccct cggtattagc tcaaaaaacg gcaaaatatg aggaactatt ttccatttgt
      181 ttctctaata ctcaggacaa aacgctgatt tcacaacctc cgatatacgg tgttccagaa
      241 gtagcttctt cgaaaaataa gctgagattt cacaaaaatt tgagaagcaa ttttcgtaaa
      301 ttccttctta tgtcttgaag ctgaccactt ctgtcataac ctctttttac tgttcttaaa
      361 cacggataaa catccgcatc tcattttatg agtgcttaga tactttgata cgattgattg
      421 ctcggtgcaa cgcaacagtt gcccgtttca attcgttcgt atcttgtttt gcttttgctt
      481 cttcgatttg gcgttctgcg cgttgttttg cacgttccgc acgagataca tcgatatcac
      541 gttcacgttc cgcactatca gcaacgattg aaacaacatt atcacgaact tccatgattc
      601 cgccattaac agcgatccaa tcgacgtggg tatctgaatc tgttcgtttt acacgaactt
      661 catcaatcgt caatggaacg atgattggtg catgtttagg caaaatccca agctcaccag
      721 ctgtcgtacg tgcaacgacg atagctgcat ggtgatcata gaccaaaccg gcaggagtta
      781 ccacatttac tgttaaatat tgatccatgg ggcacccctt tctagtagcc tagtgttttc
      841 gctttttcta cgacgtcttc gattcggcca acacttcgga aagcttcttc aggaagatca
      901 tcgtatttgc cttccaagat ttcgcggaat cctttcactg tttcttctac agggacatat
      961 gaacctggaa gaccagtaaa ttgttcagcc acgttaaagt tttgtgacaa gaagaattgg
     1021 actctgcggg cacgagaaac taacactttt tcttgatcgg ataattcatc catacctaag
     1081 atagcaatga tatcttgtaa ttcacgatat cgttgcaata cgtgttggac ttctgttgct
     1141 actttataat gttcttctcc cacgatttca ggtgctaaag cacttgaaga agaagccaat
     1201 ggatctaccg ctggataaat cccttgttct gttaatttac gttccaagtt ggttgtcgca
     1261 tccaaatggg cgaatgctgt tgccggcgcc gggtcagtgt agtcatcggc tggcacatag
     1321 attgcttgga tcgatgtgat cgaacctttt ttcgtagatg tgatacgttc ttgcaattga
     1381 cccatttctg ttgccaatgt tggttgataa ccaaccgcag aaggcatccg tcctaacaag
     1441 gctgatactt cagatccagc ttgagtgaaa cggaaaatgt tgtcgataaa caacagtaca
     1501 tcttgacctt ctacatcacg gaaatattcc gcaatcgtca aaccagtcaa agcaacacgc
     1561 atacgtgcac ctggtggttc gttcatttgt ccgaacacca tggctgtttt ttcgataact
     1621 cctgaatctt tcatttcata atacaagtca ttaccttcac gagtacgttc acctacaccg
     1681 gtaaatacag aaataccacc atgttcttgg gcgatattat ggatcagttc ttggatcagt
     1741 acggttttac caacaccggc accaccgaat agtccgactt tcccaccttt taaatatggg
     1801 gcaagcaaat cgataacttt gatccctgtt tccaaaattt ctgtacttgt acttaattcg
     1861 tcaaaggctg gtgctttttt atgaatttcg cttctttccg catcttctgg gaaaggtgtt
     1921 tctaaatcga tcgtatctcc tagtacgttg aatacgcgac ctaatgtttc tttacctaca
     1981 ggaacagaga ttgctttgcc tgtatcaatg acttccattc ctctttgtaa accatctgtt
     2041 gattccatcg caatggtccg gatcactccg tcccctaatt ctaacgctgc ttcaagaaca
     2101 acttttgatt tattttcgtc atttttataa acaactaatg cgttgttgat atctggtaag
     2161 gattggtcta aagaaaattc cacgtcgaca acgggaccga ttacttgaac aatcttgcct
     2221 gaactcatct tttttcctcc aatctcaata ctattctagt gccgaagctc cggcaacaat
     2281 ttcggtaatt tcttgggtaa tagccccttg tcttgcacgg ttgtaagaaa ccgtcagctc
     2341 atcaatgata ttagcagcat tatcagttgc tgttttcatt gctgtcatac cagcagcatg
     2401 ttctgctgtt ttggcgtcaa cgatcgcacc gtagatcaaa ctttcagcat actgaggcaa
     2461 taactgagac aagatttctt cttttgaagg ttcaaaaata tattcttgtt cgaaagtagt
     2521 tgcttcttct ggatctaagt ctgagattgg cagcattttt tctacacgaa actgactagt
     2581 tagcgaattg atatgatggt tataacatac gtataattca tcgaatactt cgttttggta
     2641 catagttgtt gccatattta cgattttgcg gacttcatca aagcttggct gatcagatag
     2701 attgcgcagc tcatatgcta gattgatacc gcgtgcttta aagaagtctg caccagttcc
     2761 gccaattgcg atcatcacgt aatcatcggg tgattggtgg tcttcttcta aaatagacat
     2821 cgtttgtttc aatatcgagc tgttatatcc accgactaaa ccgccatctg cagtgatgac
     2881 gatataaccc gttttcttaa caggacggct gagcaacatg ctgttgtaat tgatatcccc
     2941 tctaggattt tctgaggcaa tatcatttaa ctgagtagca gtcaaatgcg taacgatttc
     3001 tcttactttt gttgcataaa cctgaaactt ctgtgaagaa gcttcagatt ttgtcaactt
     3061 agaagcagac accatctgca ttgcccgcgt gatttggctc gtttttttcg ttgaagcgat
     3121 acgtgtcttg atttcattta atgaagcacc cattcatctc acctcactat gcattttgaa
     3181 tggatttcaa tttatcttct gtagaggaat tatttccttt tgtagctaag aacatgtctt
     3241 tatattcctg aatcgcactg ttcaacgctt cttcttctgg aagatctttt gttgtacgga
     3301 ttgtttcaaa gatatctgaa tgattggtat ccagatattc gaataattca tgttcgaagt
     3361 ctaaaatact gtctacaggg atactatcta agaatccatg tgtcaaagca tacaaaatca
     3421 ctacttgttt ttcaacagct agcggtgcat gcaatttttg tttcaagatt tctactgtac
     3481 ggcgtccacg atttagtttt gcttgtgttg ctgcatccaa gtcagaaccg aattgtgtaa
     3541 atgcttctaa ttcacggtaa cttgctaagt ctagacgtaa agtcccagca acttttttca
     3601 ttgctttgat ttgcgcagaa ccccctacac gagatacaga tagtccggca tcaaccgctg
     3661 gacgagtacc tgcgtagaac aagtcgcttt ctaagaagat ttgtccatca gtgatcgaaa
     3721 taacgtttgt tgggatataa gcagagatat ctccagcttg tgtttcaaca aatggtaagg
     3781 cagtcattga accgccacct aaatcatcac ttaattttgc tgcacgttcc aataagcgtg
     3841 agtgtaagta gaaaacatcc cctggataag cttcacgacc tggtggacgg cgaagcagca
     3901 aggaaagttc acgataagca acagcttgtt ttgaaagatc atcaaaaatg atcaagacat
     3961 gtttcccgtt gtacatgaac tcttcaccca ttgcagtacc agcatatggt gcgatataaa
     4021 gcaacggcgc tggttgagac gcaccggcat tcacaacgat tgtgtaatcc attgcgccat
     4081 attttttcaa tgtttcaact tgtgtacgaa ctgtagaatc tttttgtccg attgctacat
     4141 agatacagat catatcttga cctttttggt tgatgatcgt atcgatcgca atggaagttt
     4201 tccctgtttt acggtcaccg ataactaatt cacgttggcc gcgtccgatt ggtacaagag
     4261 cgtcgattgc ttttagacct gtttgcattg gttcgttaac agatttacgt tgcataacgc
     4321 ctggtgccat tgcttctact ggacgagctt tgtcagtaac gatttcacct agtccgtcaa
     4381 ttggctgacc tagcggatta actacccgtc caatcaaggc ctctccaact ggaacttcca
     4441 tgatctttcc tgttcgtttt actttatctc cttcacgaat ggactcgaaa tcgccaagga
     4501 taatgatacc tacatcattt gattctaagt tttgcgccat tccgtatgag ccatttgaaa
     4561 attcaagcaa ctcaccactc atcgcgtttt ctaaaccatg tgcacgagcg atcccgtcac
     4621 ctacataagt tacggtaccg atttcttcga ctgaaagcac gttttgataa ttttcaattt
     4681 gttctttaat caaggcacta atttcttctg ctttgatggc cattcgattc acctacctct
     4741 ttgtactatc tatttaattg attacgcata ttttctaatt gcgtgcgaat gcttccgtcg
     4801 attactcgat ggttagcttc aacaatcgcg ccaccaataa tagaagaatc aacgatttgt
     4861 tttaattcga ctgattgata gttcattgtt tttgcaacat tcttttctaa ttgtattcgt
     4921 tgttcgtctg aaagtggaac tgctgttgtg acacttccca acagcaaacc ttgatgttca
     4981 tcatatcgac gttcatattc ttcgatcatg aacaaaagat catccattcg gttatacgca
     5041 taaacaactt ctaaaaagtt tttaacgatt ccatcatagc cactaacaag cttgtccatg
     5101 atttctcgtt tttcatgagg ttctagacga acatcactta acatattacc taatccaggt
     5161 acttgttcat agatttgacg taagcttacg agatcttgat aaatttcttc tgctttattt
     5221 gaatcgatcg ccagttcaaa taaagctttc ccgtaacgtc ttcctactgt atatttatct
     5281 agtttcattt gaagaaccta gaccttcgat atattgattg attaatgatt cgtgcatttc
     5341 tggagataat tctttgttca agattttttc cgcgatttga agagatagtt ctgcaacatc
     5401 gtcttttaca gagttcagcg ctgaatcacg ttctacagtg atatcagctt gggctttact
     5461 ttttagacga gctgcttctt cttgagcatc cttcaaaata ttttggcggc ttaattctcc
     5521 actttctttc gcatttttga tgatatcagc tgcatcagaa cgagaggcta acaattgttg
     5581 ttcgcgttct tgttccattt tcgctgagtt gatgcgagat tgttctgcag aatccaaatc
     5641 attggcgatc ttgtcttcac gttttttcaa aatatcgctg attggtcccc aagcaaaatg
     5701 cttcaagaga gccagtagta tcaaaaatga gccgctgaca acgatgacat ttcctaacat
     5761 cgcattgcca acttctgcaa ttgccaattg attcagcata ggcgagcctt cctttctttg
     5821 aagtacttct agttttcatg taaaaaagca gtaacaaacc aaattgtttc tgccttaata
     5881 ctgattcaat taaacaccaa aaactagaat caaggcgaca actaccccta agataggaac
     5941 agcttcgact agagctaccc cgataaacat tgttgtacgg aattgaccag acatttcagg
     6001 ttggcgagcc attgattcga ttgttttaga gataacttga ccgttaccat aaccggctcc
     6061 gattgctgct cccatgattg cgattgctgc tgcgatataa ttcataattg ttttcctcct
     6121 aaattagata aatagtagtt tgtttttaaa acgaatatta ttcttccact tcaatcttat
     6181 ggctcatata aaccatagat aaagtaacga agacaaatgc ttggatgctt ccgataaaca
     6241 aggagaatgc gatccagacc atctctaatg gaattgctaa cggcaatgag aaccagccta
     6301 agcttattac catttttgca atcaaggtta gcaaaacttc ccccgcataa atattcccat
     6361 aaagacgtaa agcaagtgtt aataagtttg taaattcttc aatcagtttc atcggaaaca
     6421 taaaactata tggtttcaag aacgaattta cgaaatatcc tttgaagcct aactttttca
     6481 ccccaaataa gtgagtaagt aagatcatta taaatgccaa agttaaagta acaaacgggt
     6541 ctgctgtcgg gcttttccaa agtgttgttt cgttaggtaa aacgattttt gttactaaac
     6601 cgatgttgtt tgcaacaaaa acaaataaaa acaatgtaaa tgccaacaaa tggaaattgc
     6661 tcaattcttt tctaggcaga ttatctgtta cgattcctcg actaaaatca atgacccatt
     6721 ctatcgcatt ttgtttgcct gttggtttca acttaagatt tcttgtaaaa aagtaagcta
     6781 agaagaaaac aatcaaacag gtcagtaaca ccattataca aactgtacca tcaaaccaaa
     6841 ttggtccaat gtggaacgtt agcgagcgtt catccaatcc atttcacctc ttttccccga
     6901 aattcacata aaatcaattc atgtgcgtat ctggtttttt ccaatcggtt tctaaccaca
     6961 cggtatcata ccaccatgaa acagaaaatt caaaacattt tcatgaaaat tagacacttt
     7021 tttatttcag tcaaaaacga agcgaatttt tctttataca cggtgctcct gatctaagaa
     7081 aaaacctatg ataatcatgt tttatcagat taaatgctaa caaattttat cattaagtaa
     7141 aagcaaaagc accgaatatg cgaaaaagga taatgaaatt atctagagaa attttttaat
     7201 gttcgatcaa tttgtcgatc gatatctttt cgtttcaaat cttcccgttt atcataagat
     7261 tttttccctt tcgccagacc aatcaatacc ttggcatagc cgttacgaat atagactttt
     7321 aaaggaacaa cagtgattcc agtatttttc aattcatttt caagccggat gatttgtttt
     7381 ttgtgtaata ataactttct cgtgcgcaac ggatcatgat taaaaatatt tccttgttca
     7441 taaggactga tatgaacatt atgcaagaaa gcttctccgt tgcggacgcg aataaatcca
     7501 tcttttagat tgatccggct gtttcgtatc gacttgatct cggttccttg caagaccatc
     7561 cctgcttcca tcgtgtcgat gatcgaataa tcatggcgag ctttcttgtt ttgtgcaatt
     7621 aattttccct cgccttttgg catagctgtt tcctccaact cattttattt tttctttttc
     7681 ttctttttaa cacctttgta aaatggcttc ttcccttttt tcttaccgaa cgtttgctta
     7741 ctatcgctat atcgtttgtt cttcttgcca tttctttctg ggcgttttcc tgcttgtttt
     7801 ccttcgcgtt ttcctgattt tccatttcgt gtttttggcc gttccaacgc tgtaacctct
     7861 tctgcggaaa caagttcaaa atctactgct cgttcatctg gatcagcttt gactactttt
     7921 actcgcactt tctgtccgat tttaaaagtc agaccagttc tttcacctac aagagctaaa
     7981 tgattttcaa caaaatggaa gtagtcctgt ttcaattcat tgatatggat caatccctct
     8041 actgtattcg gcagttcgat aaacaggccg aatttcacaa cagagctgat gatcccatcg
     8101 aattcttccc ccactttgtc catcatgaat tctgctttct tcatggaatc tacttcacgt
     8161 tctgtttcta ctgcacgacg ttccatctta gaactatgat ctgcaatttc aggaagttcc
     8221 gtagcccacg aactttgatt gctttcactt ttatcttcac tgtaagaacg gatcagacga
     8281 tggacgatta gatcgggata gcgtcgaata ggagatgtaa agtgtgtgta gtaatctgcc
     8341 gctaagccat aatgcccaaa attgtcttct gaatagcgtg cttgttgcat gcttcttagc
     8401 aacatagtat tgataactgc agcttctggt ttgtcttcta ctccttcgat cacatgctgt
     8461 aaatcttttg gtgtgatcgt tcctttcgtt cctcggacca gaatgcccaa agctgaagca
     8521 aaatcaaaga agcgctgcat tttttcttct tttggctgtt catggattcg gtaaataaac
     8581 ggcagattta atttattata atgttcagct accgtttcat tggccgcaag catgaaagat
     8641 tcgatcaagc gttcaccaat tcctctgctt cttagctgga tatcatttgg gtgtccttca
     8701 gcatcaacaa ggatttttgc ttcgcgatcc tcaaaagaaa tcgcgccacg tttgattcgc
     8761 atcgtttcaa ggattcgatg caattcaccc atttcttgga acataggtac gagctgagca
     8821 tagcgttgca tcgtttcttc gtcttgatct tctaagattt gattgacagc tgtgtaggtc
     8881 atccgttctg ttgtttggat cacacttggg aaaatatcat gagagactac ttgtccattt
     8941 ggatcaatct ccatttcgca actcatggtc agccttggaa catggggatt cagtgaacag
     9001 ataccattcg ataaacgttg cgggatcatc ggaatcactc gatcagtcaa atatacactt
     9061 gtccctcttt catatgcttc tctgtccaat tgactgcctt gtgtcacata gtaagaaaca
     9121 tctgcaatgt gtactccaag aaaataatta ccgttatcta attttttaac cgtaactgca
     9181 tcatctagat cttttgcatc ttctccatca atcgtaacaa tgagctgatt tcttaaatct
     9241 ctacgtccag ttatatcctt ttcgctgatt tgatctggaa cttcatctgc ttcagacaat
     9301 acatcatcag gaaatttcgt agggatacca ttagccacga caattgagag gatatccatg
     9361 ccagggtcat tcttatgacc aatcacttgc ttgattatcc cttctaaact agtcgcatac
     9421 cctttttcag ggtaatgggt cagttcgatc agcacaatac ttccgtcaac tggacgaact
     9481 ccctctgctg ctgcatatac tttaaattgg ttgagctttc tatctttcgg gataacaacg
     9541 ccgtaaagat ccgtttcttg tatttcttct tctgaataag caacgaattc ccctgctaac
     9601 tgagtagtcg cccggtctcg aatctcgact actttcccct ctgctccgcg atccatatct
     9661 gtatttgctc gcttcatgat atcgattgct acggtgtctc catccattgc aaaaccagtc
     9721 gcttcttttg ggatatagat gtcatcttct tcgggatcaa tcgttacaaa tccaaatcct
     9781 cgttcatttg ctcgaaatgt tccttcgatc aagattggtt ttaaaggaag tttcactttt
     9841 ccttttttat tgaaaactat actctgttct ctttccattt gagcaatcgt ttgtactagt
     9901 aatttaaaat catcgctttt ttggaaccct aaaccttcag cgatttcttc catagaaaaa
     9961 ctgcgtttgc gactgctttc catgaaaaag agaattttct cttttatcgt ttcttttgtc
    10021 atatttattc ctcattccaa ggtaattttt ctaaaaattc caagacgtct ttttctaatt
    10081 cgcgtcttgc tgtaccaact gtaatcacat gaccactttc aggataccat cgaagtgtca
    10141 ctcgctgacg gctcaacttg cgtgcagttt caaatacgcc attagggtcg atcatttcat
    10201 ctttcccagc ttgagctaaa aatatcggct gttggatatc tgacaaccta ctttctacga
    10261 ttgctgcctg atcttgtata tccattaatt gttgttcagc taacgagcgg taagcaactg
    10321 ctttttcatt gatttctttc tctgatttac ctgctgtctt cagcacttga cgtacgtatt
    10381 tttcaaagtt ttcaggcacg tttgttttta caggagacaa aggcgaacag aaaaaaccgc
    10441 cgccaatcac tgattcttct tctagtgctc gtacagcaaa gatgccgccc attgataatc
    10501 ctaatacggc aatatcagaa aactgctcag actgtaaaaa atggatggct tttttagtgt
    10561 ctgcccacca gctttctgct ttttcttgca aaatatcata tggctccatt gttccgtgtc
    10621 ctttaaaaag cggcgcataa actgtataat ctgctttttc caaaaatcgt gcgagcattc
    10681 gaacatcgtt tggacttcct gagtaagcgt gaagcagcaa aaccgctcgc tttccatgtt
    10741 ttgcataaag gggttttggt aattgtctca ctcgtctcac ctctttcgcc tcattttacc
    10801 atagtttctt tcataacttc ttcttaagcg ttctctttgc aataaaaaaa gccgcccgcc
    10861 gttttgcgag aggcttattc aactacttcg aagataaata tgctaggaca aataataaga
    10921 tcatccagat agctcccata actgcagttg atcgctgcat gacagcttca aagccgcgag
    10981 ctttttgttt gccgaatagt tgatccgcac cgccggtaaa tgcactcgct gcactatttt
    11041 gcttgcttgg ctgcatcata atcgcaatga ccataacaat cgataaaacg atcacgattc
    11101 ccaaaattaa attatacata tcgcagcctc cttcccaaaa gatatcgtct ctttaacttt
    11161 atcatagata aaagtccgtg acaactctat ataaatggaa taacagctga aagttcaaaa
    11221 ctttcagctg ttattcttat gactgacgta agacgtcttg atattctggt ttcttttcaa
    11281 actctttttt tgcataaggg cataaaggaa tgatcttttt cccgtctcgt cttgctaatt
    11341 ctactccagt atacactagc ttttcagcta acttttgacc acgatatgct ggatctacaa
    11401 atgtatggtc aatgatcatg atgtctgggc ccgcatctga ccatgtcatt tctccgatct
    11461 cttgttgttc atcattgaat aaagcaagac gatttttttc ttctttgatt tccatatgcc
    11521 gcactcctca aataactgat atttttttat tcctacctta ttttagcagt ctcctgatct
    11581 ataaacaatt atttatcctc gaattactta caaaaaagaa gagaaaatta attcatcgtt
    11641 attctttgtt caataaatga aaaaatcact gtccaatagg ttttcggatc tttcatcgat
    11701 gcttccaagt gttctgcttc tggaacaagc agcagtgctt tatcccctgc cgtagcattt
    11761 tgaatcgtgt acgctgcttc tgtagggaca aaatgatctc tttcaccatg aatgaccaat
    11821 aaaggcagat ggttgctgcc aagctgtctc gtagctgatg cttgctttaa ggagtaaccg
    11881 gcgtatttct gggcataatg attcgcatat ctcatgacca tttttttagg aaagacagtg
    11941 attttactta acatatatcg gaattctgca taaacagaaa catatccgct gtctaaaata
    12001 aatcctttga ccgctgatgg aagtttttca ccacttgcca tcatgatcgt agaagctccc
    12061 attgatccgc cgtgtaaaat aatggacgca ttcggctgtt catctaagat gagttggatc
    12121 caggcaatta aatctagccg atctagccat cccattccga tgatttctcc ttcactttct
    12181 ccgtgcgcac gtaaatcagg tacaagaacg ttgaatcctt tctctgcata tcttttacct
    12241 atatgagaca tgtctctttt ccctgtgctt cggtaatcat gtacgcaaat gacccattta
    12301 ttctgcttat ttggatggat gaacatctga ccagctagtt tcaacccgtc ataacttttg
    12361 attgttttgg gtgttccctg ctcccaaaag ctttcacact cttcttctaa tttttgtcgt
    12421 tttttttcta ctgacgtgac tgcagcaggg tccatcaaac gatgaccagt gtcttcatac
    12481 cagtcatatc ctctctgtac tcccgctctt actaatttct ttgcataaaa ataagaaaga
    12541 aaatggtaac tgatccctat aattatcaga ataagaatga aataaaaaat aaccattgta
    12601 cacccgccgc tttctgccaa actataatct aactattatc caatgtaccg atccatagga
    12661 ttctttattt tcggcacacg taaattccat ttctttttca ttgtatcatt tacttaaagt
    12721 tctcctcaat agatttactt gtaagagagc atttacattc gcaataaaag ccagtatact
    12781 aatttcgtgc attataatca agacgctcgt cttggactgg ttcggaaact tcccagaata
    12841 aaaaactaag tatctctaaa gaaaggagat tggaaataat gcgaaaagta attattgatt
    12901 gcgatccagg gatcgacgac actttagctt tatcattagc tgtcaaatcc cctgatatcg
    12961 aagtagttgc tattaccgta gtttgtggga atgtccctgc agatataggt acgcagaatg
    13021 tgctgaaatg cctggaacgc tgtgatcgtt tagacattcc tgtttatcaa ggaatggaaa
    13081 aacctttgaa gcaacctttc atcagtgcac aagacactca tggtatggat ggtttagggg
    13141 aaacaaattt cccgatgatc ttgagaaaac aggcagaacc tctccatgcc gttgattttt
    13201 tggctgatta tttcaaagaa aagacaaata catcagtcat tgctttgggt cctttgacca
    13261 atatcgcttt tgctttgaaa gtcaacccaa atattgggaa acatatggat cgttttgtct
    13321 cgatgggtgg aacttacaaa agccatggta actgttcgcc agttgctgaa tacaattact
    13381 ggtgtgatcc tgaagcagct tcttatgtat tcgagaatct caaacaaacg atagaaatgg
    13441 ttgggttaga tgtgacaaga gaaatcgtct tgacaccgac tatcctcgaa tactgctgtc
    13501 aaatgaatcc tgaagaggga gagtatctaa aagcgatcac tcgtttttat tttgacttcc
    13561 actggaaaca agaaagaatt ttaggatgtg tcatcaatga tccgttagct gttgcttatt
    13621 ttatcgaggg ggccatctgt gaaggtttca aaagcttcac tgctgtggaa actcaaggaa
    13681 taagtcgtgg acaaacattg gttgatcgtt atgaattttg gcagaaacct gctaacagta
    13741 aaattatgac aaaagtagat actcgtcttt ttttccgaaa gtttttaacc gttcttttaa
    13801 atgcacaaga agaaacgatt atgaaagatt tggaaaggtt aaagatgggc taacaaaatg
    13861 aataaaaaca aaatggcaac tcgcgtactt acagtgatgg ctttgtgtat tgggatcaat
    13921 tatattggag gaacgattgc tttatggctt cgtttgccaa tctatctcga ttctatcgga
    13981 acgatttttg ctggtgcatt gcttgggcct gtacctggaa tgctgacagg attgtccagc
    14041 ggtattttaa gtggagtgac tacagatatt ttttctctgt attattcgcc aatccaaatt
    14101 ctcacaggct tgcttgctgg attgattttg ccaagatatc tcggcaaaga taaaaacagc
    14161 ttgaaactgt tattactcgc tttgattttg tcggtacctg gaacgatcgt ttcttcgatc
    14221 attaccgttc agttatttgg cgggatcacc tcctcaggat ccagtatgat cgttcagcta
    14281 ttacatgggt taggtttaag tcagacaaca agtgtgatct tggtacaggc aggtacagac
    14341 taccttgatc gtttgttgtc tatcgtcgtt gtttcattag tcatcactat gttgccacaa
    14401 cgcttgatat attcaaaaag aaaaataggc tgaataaaca gagctggatt agcaaaataa
    14461 tgctaatctg gctctgtttt ttcatttatt aaaaaaatct tacgctaagt aggataaaat
    14521 aattccttga tatttttcaa tcatatccaa ataattatcg atttcaacat actcatctac
    14581 ttgatgaggc aaagtaacga caccaggccc aaagacgaca aagtcgaagg aatggttagc
    14641 tttcgtaaat tcggctgcat ccgttgttgc tgccgctcca actaaaggta atggctggct
    14701 gaattgttgc tgaatgcaat gaatcaaagg agaatctgga tcagctttga caggtatttt
    14761 gttgtaatcg atcatcaatt ccaaatgata gtctgtctct tgattcaatt cattgacgat
    14821 tgattgcagc aatgcaatga ttttatcatt tgggtattct ggaatcgatc gaatgttccc
    14881 ttggagctgc gcatggcttg ggatgctgtt gacttgattc ccgccactga tcaatgtaac
    14941 attatggatt gtttttccta ggacaggatt ctcgattgtt tcagcgaggt gattcatttc
    15001 agcatttgct ttcgtaatga attcatttag gtgattgatt gcattataac cttggtctgg
    15061 catagagcta tgcgcttctt ttccgtgcga ggtgactgta tagttgattg atcccatgtg
    15121 cgtatacatc aaactgtaat tagttggctc tccgatgatc aaggcatcta agtcatctac
    15181 atagcctgct ttcgtgagct gttccccacc tagttcaccc acttcttctc cgacagtggc
    15241 taataatttg actgttccgt taaatggttt tcctgactct ttcagttcga tcatggcaat
    15301 caccattgct gccaagccac tcttcatatc tgtagcacca cgtccgtaca aacggttccc
    15361 atggatttct gctgcaaagg gtgcataggt ccaagacgat tcatcaccag ccgccacgac
    15421 atccatatgc ccagataggc ctaacacttt tcctgattgg cccttttggt aacgagcaat
    15481 caaattatct ctaccatctc tgtaagaaac aatctctcct gttatgtcat gctttgccaa
    15541 aagcttattc aaataggctg ctacttcacc ttcgtttcca ttcactgatt taattcggat
    15601 gatctcttgt aaaatcgcga ttttttcttc ttttttcatc taatcctctc ccttgccatt
    15661 tctttttatt gtatttgagt gtgctcgaat tttcaaatag gagcaaccaa agtaaaaatg
    15721 aagaaagaga gccactttat gaaaaatatg ctgctacttc cctcctattt tatttatacg
    15781 agaggacttg ctcatagatt cctattctac tgttctatcc ttaaaagctc ttatatgatg
    15841 ctagttattg ttagctttcc tttttcaagg atgatctttt cttgtaccat tctttgtaaa
    15901 catcatatac agaagctagt tgcaaaaaaa actctctacc tgctgatat
//
LOCUS       DARBDV010000070        15230 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000070 DARBDV010000000
VERSION     DARBDV010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.070"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            278..787
                     /locus_tag="SCM56_002078"
     CDS             278..787
                     /locus_tag="SCM56_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG1110831.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="SCM56_002079"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="SCM56_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG1110832.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="SCM56_002080"
     CDS             2322..3302
                     /locus_tag="SCM56_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1110833.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="SCM56_002081"
     CDS             3424..4842
                     /locus_tag="SCM56_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEG1110834.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="SCM56_002082"
     CDS             4913..6382
                     /locus_tag="SCM56_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1110835.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="SCM56_002083"
     CDS             6392..6859
                     /locus_tag="SCM56_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1110836.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="SCM56_002084"
     CDS             6895..7767
                     /locus_tag="SCM56_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG1110837.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..10382
                     /locus_tag="SCM56_002085"
     CDS             7848..10382
                     /locus_tag="SCM56_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG1110838.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10538..11311
                     /locus_tag="SCM56_002086"
     CDS             10538..11311
                     /locus_tag="SCM56_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1110839.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11304..12095
                     /locus_tag="SCM56_002087"
     CDS             11304..12095
                     /locus_tag="SCM56_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG1110840.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12105..12596
                     /locus_tag="SCM56_002088"
     CDS             12105..12596
                     /locus_tag="SCM56_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1110841.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12614..13024
                     /locus_tag="SCM56_002089"
     CDS             12614..13024
                     /locus_tag="SCM56_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1110842.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13028..14428
                     /locus_tag="SCM56_002090"
     CDS             13028..14428
                     /locus_tag="SCM56_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HEG1110843.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14451..14825
                     /locus_tag="SCM56_002091"
     CDS             14451..14825
                     /locus_tag="SCM56_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG1110844.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5286 a         2135 c         3046 g         4763 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8641 ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt tcgattgaac
     8701 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8761 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8821 gggacaaact agacagcggt ttttggatag atccagaaat tagtatagat ttaaaaaaaa
     8881 tgtttcttaa atcactttct gaaattgatt tgattgaact ttttcaaaat cacatttcaa
     8941 ttgaagaaag catgagtcaa ttaagagata gaatacttaa agagtgtgca ggttcttctg
     9001 agtataatta tttagaaagt gacgaattta tagcattaag aaactatgtt gttaataaaa
     9061 tatcaccaat tattgattcg acaggtctat gtttattaga tgaattcttg gcggatatca
     9121 gtctatttat aatgtttatt gatgtaattg aagaaacatc aagaaatttt ttgctgtcta
     9181 agttcagaaa atttcgtatg aaaaatgata aacaaaaatt attggcaaat gagatttgga
     9241 gtagtttaga agtcgaagaa agtaaacaag aacttttgtt gaaatggata ctttttcttg
     9301 taaagaagtt ctatgtgaaa ataccagaaa cccattgtct gatagttatg cacggtaaaa
     9361 taacagcttc tgcgatcgca agggaaacaa ataagttgct gaatacatat gtttatgagg
     9421 ctttcgatat gccaattgaa ggtgagacta gtgacttaat taacctaatc aacaattttt
     9481 gtaaaagtat agatacgacg aatggactca ttctgctagt tgatatggga tcattggaac
     9541 aaatgtatga aaaaattgaa tccaatgtaa taggtgattt ggtaatttta aacaatgttt
     9601 ctacagcttt agcgattgaa tgtgggattc aagtttgcca aaaaaagcca attagtcatt
     9661 tttaccagat ggattttgat tcgtttcaag ttaaagttca gtattataaa ggattatcac
     9721 aaaagaaaaa tgtaatagta tcttgtctct ctggtgaggg gatttctgaa aaagttaagg
     9781 atattttaaa acggtatctc gataatcaag tggagatttt gatttttgat tttaacgcat
     9841 taaagaaaat agcaaaggaa aaagatacta tgatttttaa gaatacaatt tgtgttcttt
     9901 caaccactga attttacatt caaggaatcg attgtttaaa tttagaaaat ctaattaatg
     9961 ggaatcaaac cttagaaaaa ttaaataagt atatggactc ggatagctgt gagttctgct
    10021 taaatgaatt agttaaactc tttactattg aaggcgctag tactaagctt cgctttttag
    10081 attccaaaaa agtattcaat gagatagaaa aagtactata tttgtatgaa aaatattacg
    10141 atgtcaaaat cccaagtttt ctgagaatta atctatttct acatctttct ggaatgattg
    10201 aacgaattat gattggtgat ggaataacaa atgaacattt gaaagaggga gcgcaactat
    10261 ttgatacttt tttaaatgta tcaaaagatt ttttttcaga aatcaaagat atttactgta
    10321 ttgaaatacc caaaggagag tatgagttaa tctatacaat ttttgagcag actattttct
    10381 aaatagcata ctagcgtcta aaaaaatgaa agggtgtcca aagtatattt agacgctcat
    10441 ttaattaata tttactgtat tttcccgctt tattggataa tgttagttgg cacagtaatt
    10501 gcttatataa aagtataaga agaaaggaga gaacaaaatg gcgttttggc aaacattttt
    10561 agttgtgttg tatggatttt tcattaactg ggataaggaa gggcccaaaa tcggaacctc
    10621 ccaacctgtc gttgcaggtt tcttgaccgg acttattatg ggagatattc aaactggact
    10681 ttatatcggt gggactttgc aattaatgac actaggtatt actagttttg gtggtgcatc
    10741 agtaccggat tatcaaacag cagcattgat cggtacttat ttagcaatct caactaacca
    10801 ggatgcttct ataggaataa ctttagcaat accggtagca atgttaattg tacaactaga
    10861 tgttctaaag tggtcaacaa acattttttt tcaacaaaag gcagaaaaat tcgcacaaaa
    10921 agaagattat caaaaattag agtggatgca atatttggca tcaattaata caagctttgt
    10981 atctggaatt cctgttttgt taactgtgat attaggccca acgttagtgg gaagtgcaat
    11041 caattatatt cctgaatggg ttaccggtgg tttaaaggta gcagggggac tattacctgc
    11101 tgttgggata ggaatgctac ttaaatattt acctacaaaa gattactttg cctatttgtt
    11161 agttggtttc gttttggcag tttatttcca aataccaatt ttagggattt cgttacttgg
    11221 cgctgcaatc gcactaatcg tatataaaaa tagatcaaat aatactcaag tagcaccagc
    11281 tgcaacagga ggttttgacg aagatgaata agaaactgac aaaaatcgac aaacaagtaa
    11341 ttttccggtg gattctcaat ggtgcaactt cattaaatta tgaaaaaatg gaagggttgg
    11401 gttatgcgta ttctatgctt ccttttatca aggacacata taaagatgat cctgaaggaa
    11461 tgaaacaatc cgttttagct cacttacaat tttttaacac tacgccgtat acagcgcctt
    11521 acattattgg tatgaatgtt ggaattgaaa aagaagaaaa aaataagtct ttggaagcag
    11581 taagtgctct gaaaacaggt ctaatgggtc ctttagcggg aattggagat agtgtatttg
    11641 tagttattcc atggactatt tttggagcaa ttgcagctaa tatggcattg gaaggtagtt
    11701 ttgttggaat tattttgtgg atactggcat caattttatt aaggattact gcttatcctt
    11761 tataccttgc tgggaaagaa tcgggtgcga gcctactaac tcaaattgaa acaaaattaa
    11821 agtctattac tgatgcagta tcaattttag ggttaatggt ggtcggtgca ctcattgcca
    11881 cagttgtaaa agtgaatgtt ggctttgaat ttaaacaagg cgacttaaca atgaccggaa
    11941 atgaaatctt tgatcaagtt atgcctggtt tattacctgc atgtgtagta gcaattgtgt
    12001 attatttgtt aggacgaaag gttaaaccag tttatgtaat tctattaatc ttagtagttt
    12061 ctattgcgtt aagagcgtta aatattttga agtaggtgtt gaatatggat gaaaaaatga
    12121 aaggtattat acatgttcgt attgatgatc gaatgattca tggacaagta gcaacacaat
    12181 ggacaaatca attgagtgct aacagaatta tggttattaa taatgaagta gccaatgatg
    12241 acgtaaaaaa agctgtagtt cgcctcgctg caccacctaa tgttagaact tcaataatta
    12301 caagggaagt agcagttaaa aatattcttt ctggtaaata tgaaggacaa aaagttctta
    12361 ttgttgcagt ttctccaatc gatgttaaat ttttgattga taacggttta ccaataacaa
    12421 gtgtaaacgt aggaaattta tcaagaagga atggaacaga acgcatccgt ccgacgataa
    12481 atattactga agaagagaga gaggctttta aagaactaat tagtgatgga gttgaagtga
    12541 cagtgataca aactcctaga gattcaaaag aatttttaaa ggattttatt tattaagaac
    12601 agaggatgag tggatggagt ttataattac atctcatgga aatattgcca ttggatttgt
    12661 agatacatta aaaatgtttt ttggcgataa tttaaattgt catgtggtaa cacttggaga
    12721 tgcaggcatt gaagaattcc gtgaaaacat tgaaaaaata gtttctcaag taaaaaatca
    12781 agaagttatg gtatttgctg acttgattgg tggaacacct ttcaacgaat ttgctaaaag
    12841 aagtgaagta tttcaaaatg ggtttcaact tcttggtggc atgaatttag gaattttgat
    12901 tgaggctgtc aatttgagat tacaaggaaa aaaaagcgat gatatcatcg aaaatctaag
    12961 aaatatgaat acaattgcgt gtcttgcaga tatgcaaaga catactaacg aagaggatga
    13021 gtgataaatg caaaaaattt tttacaagcc taaagatttt tggtttgggg attgcatgcc
    13081 tttttataaa gatggaactt tttatctatt tcatcaacga gatacgagaa atcctaaacc
    13141 tttaactgat ccttttgggt ggtcactagt tacaaccact gattttgtga aatttgaaga
    13201 atacggggaa gtaattcatc gtggtggcaa cgatgacatt gatcaataca tttacgctgg
    13261 aagtattgtc caaggaatca atgagtattt agctttgtat actggacata atcgcaaagc
    13321 taagttggat ggaaaaactt cagaagtgtt aatgattgca agttctaaag atttaattca
    13381 ttggaaaaag gatggcgaag cagcaaaact tgttcctcaa gaaggatatg ataagaatga
    13441 ttggcgcgat ccttttgcaa tttttgatca agatcatgga aaatacgttt tgatactagg
    13501 agctagaaaa ccagcaagta agaaaaagcc gacggggaga ttggtctatt ttgaatccga
    13561 agatttaaaa gattggaatt tcaaaggaga ctactgggct aataatgaat ttaacatgat
    13621 tgaaatgcct gatatatttg aaattgatgg aacgtggtat ttactttttt cagagtattg
    13681 tgaagataaa aagactaaat atcgagtcgg agcaggatta ttttcagatt ggaaatcacc
    13741 agtagacgaa gctttcgacg gaaaagccta ttatgcagca agaacagtcg gaactaatga
    13801 aactggccgt tttctaactg gctgggtggc aacaaaagaa ggggagaacg atcaaaataa
    13861 ctgggattgg ggcggtgcac ttgttccaca tcaaattatt caaaagacag acaaaactct
    13921 tggtgttaaa ctgcctgaaa aaataattga gaattttaaa gaaatcactc cattttcaag
    13981 ttttaagtta gagaaagaat ttggaaaaaa agaaagagta attgttccta aaatcgaaga
    14041 ttattttcta tttgaaacaa ctttgactat tacatcgtta acaaaggagt ttgctttaaa
    14101 attatttgta gatagtgata ctcaagaagg atacgaattt cgtatttcat tgattaataa
    14161 tcagcttact ttcggaaaaa ctccgaacta tccttggcct caaatgtttg ataaggggct
    14221 gcaacgacca ctatttttgg aaataggcaa agaatatgaa ctaaagttaa ttgttgatga
    14281 tacgatagct gttttatata ttgataatgt tgctttaagt actagaatgt atgataaagt
    14341 cggaacagca ttaagtatga ctgttacgga gggagcaatc aaggtagata aactagacta
    14401 ttcaataaat tatgaatgtg gattttaaaa caattgaagg gatgatgtaa gtgaaattaa
    14461 atggtttaca acatattgga attccaacaa aagattttgg agcttcaaaa atcttttatg
    14521 aatccttagg gtttagtctg attaatgaag aaatgaatgt gggaagtaaa gtcggctttt
    14581 tcgagttgaa tggaacgata atagaaatat gggaagataa aactactgag tgtagaggag
    14641 ccattgatca tctagcatta gatacggatg acatagatga ggtattcaat tcaattcaag
    14701 atttaggata tgaattatta gataaaaata ttatggaatt accgttctgg gaaaatggaa
    14761 tccgttactt taatttctac ggaccaaatc atgagattat agagataagc caaatgaaga
    14821 aataaataga taacgaagtg ctctaagttg gtatgaacga aaagtacttc attttttggt
    14881 atcagcattt caatagttta atgagaattt actatttcaa attaattcaa agtgacagta
    14941 ttttttactt atttggtttc aaattttgta atccttccta tttattggaa tacactccac
    15001 attttgtata caagtcatat catgttttag caaagctctt accaagcaaa gttataatga
    15061 tgttttccaa agtttgatct ttcgatggag gctaacacat attttttgca ggacagccaa
    15121 aattagatgt atagaataga gtctgtgagg cgtatagaca gtttattgtc tgtatgcctt
    15181 tttttatttt gaaaaatttt tagtattttt cgaaaaatgc tcactcctag
//
LOCUS       DARBDV010000071        14815 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000071 DARBDV010000000
VERSION     DARBDV010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.071"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(296..1024)
                     /locus_tag="SCM56_002092"
     CDS             complement(296..1024)
                     /locus_tag="SCM56_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HEG1110845.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="SCM56_002093"
     CDS             complement(1002..2576)
                     /locus_tag="SCM56_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEG1110846.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="SCM56_002094"
     CDS             2805..3743
                     /locus_tag="SCM56_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1110847.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="SCM56_002095"
     CDS             3755..4666
                     /locus_tag="SCM56_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1110848.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="SCM56_002096"
     CDS             4690..6162
                     /locus_tag="SCM56_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEG1110849.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="SCM56_002097"
     CDS             6213..6824
                     /locus_tag="SCM56_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEG1110850.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="SCM56_002098"
     CDS             6854..8767
                     /locus_tag="SCM56_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HEG1110851.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="SCM56_002099"
     CDS             8895..11876
                     /locus_tag="SCM56_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HEG1110852.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="SCM56_002100"
     CDS             11873..13534
                     /locus_tag="SCM56_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HEG1110853.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="SCM56_002101"
     CDS             13574..14506
                     /locus_tag="SCM56_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEG1110854.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4815 a         2333 c         2978 g         4689 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagt
//
LOCUS       DARBDV010000072        14562 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000072 DARBDV010000000
VERSION     DARBDV010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.072"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(367..729)
                     /locus_tag="SCM56_002102"
     CDS             complement(367..729)
                     /locus_tag="SCM56_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110855.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(803..1183)
                     /locus_tag="SCM56_002103"
     CDS             complement(803..1183)
                     /locus_tag="SCM56_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEG1110856.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1247..2440
                     /locus_tag="SCM56_002104"
     CDS             1247..2440
                     /locus_tag="SCM56_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEG1110857.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2517..3101)
                     /locus_tag="SCM56_002105"
     CDS             complement(2517..3101)
                     /locus_tag="SCM56_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEG1110858.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3302..4300
                     /locus_tag="SCM56_002106"
     CDS             3302..4300
                     /locus_tag="SCM56_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEG1110859.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4412..4921
                     /locus_tag="SCM56_002107"
     CDS             4412..4921
                     /locus_tag="SCM56_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEG1110860.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4951..5610)
                     /locus_tag="SCM56_002108"
     CDS             complement(4951..5610)
                     /locus_tag="SCM56_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEG1110861.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5612..6376)
                     /locus_tag="SCM56_002109"
     CDS             complement(5612..6376)
                     /locus_tag="SCM56_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEG1110862.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6384..7061)
                     /locus_tag="SCM56_002110"
     CDS             complement(6384..7061)
                     /locus_tag="SCM56_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEG1110863.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7098..8483)
                     /locus_tag="SCM56_002111"
     CDS             complement(7098..8483)
                     /locus_tag="SCM56_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEG1110864.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8693..8777)
                     /gene="ffs"
                     /locus_tag="SCM56_002112"
     ncRNA           complement(8693..8777)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="SCM56_002112"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8800..9315)
                     /gene="tadA"
                     /locus_tag="SCM56_002113"
     CDS             complement(8800..9315)
                     /gene="tadA"
                     /locus_tag="SCM56_002113"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEG1110865.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9422..10279
                     /locus_tag="SCM56_002114"
     CDS             9422..10279
                     /locus_tag="SCM56_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1110866.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10360..10695)
                     /locus_tag="SCM56_002115"
     CDS             complement(10360..10695)
                     /locus_tag="SCM56_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110867.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10762..12132)
                     /locus_tag="SCM56_002116"
     CDS             complement(10762..12132)
                     /locus_tag="SCM56_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEG1110868.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12210..12746)
                     /locus_tag="SCM56_002117"
     CDS             complement(12210..12746)
                     /locus_tag="SCM56_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1110869.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            13055..14536
                     /locus_tag="SCM56_002118"
     CDS             13055..14536
                     /locus_tag="SCM56_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1110870.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4485 a         2897 c         2366 g         4814 t
ORIGIN      
        1 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcgt
       61 tagtaacatg tcaataacta ttttttaaat tttttagaaa tgtgatataa catccacttc
      121 agaatcaacg ctgttaaatt tatcatgtat ttcttccttg gtcaactata aattaagcat
      181 tactttgtga aatgattttt cgatcaatta ttattattcg ttatacaacc gttttcaaca
      241 tattttgaaa ataaaatgca ctaaaaaact atctttagca gaacatttca ttgtattttt
      301 tggaccaaac aataaaaaaa gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc
      361 tgtattttag aagaaaggca ctccctctac ttcgtaagtt ccaagaacag agcgcatttg
      421 acctggttct tcccaaatat atccttcgcc taattcttct ctttgattct tttgttcagt
      481 ttcaaagaca aacaacggaa cattttcctc tttaatatgt gtatttgtca attccatcaa
      541 actaatcttg ctaacatcga tatgaactat ttcttttaag aaattaagta tattcgctaa
      601 accagtcttt ccttcagaaa gttccgctaa cgcaaattcc aacttgtcat caattgaatg
      661 gaccaaaaat tttttagtac catcctccaa atgcaacata attgtaccag caactctttt
      721 gcttgccaaa atttccacct cgatcaactt gttctatggc ttacataaaa attctagcac
      781 attttcagaa aaaatacact ttttatttct tattttcttt ccgttctagt tcagataatg
      841 cttcttctat ccaaataggt aactgctccg caatagtggt aaatccgatc ttacgatttt
      901 tattcgtgaa ataccttttt ctgcgatatg gaacagctgg atttgttttt tctaacgttc
      961 ttaaagataa actgattttt ttggaatatt catcaatgtc gatgatctga actgttactg
     1021 gctgtccgac ttttaacaat gaatggatat ttttcgtata accggattgt acttctgaaa
     1081 catgaataag tccttgcgtc tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc
     1141 ctgtcacttt tccttcaatt atttgtccaa ttttataagt catctacttc cctctcttcg
     1201 catttatcat tatactgtaa atagctcaaa aaaagaagcg acgaaaatga ctggaggtct
     1261 taagatgatc gaatttgata aacagatttc aagaaaagat acagaaagtg taaaatggga
     1321 cgcaattgct gagacatatc aaatggatga cttacttcct ttgtgggtag cagatatgga
     1381 cttcttgtct cccggtgggg tagctaaagc gtttagtgaa tacatcaaac atggaatctt
     1441 tggttatgcc actctgtctg ataaactata tcaggctatc attcattggg aacgccaaca
     1501 ccatgctgtg gagttgacga aagaaaatat cgtttttaca agtggtgtgt taaccagttt
     1561 agctgcagca gtacaaactt ttacaaaacc aggcgattct attttgattc atgacccagt
     1621 ttatcctcca ttttcctcta ttatagaaac aaatcaacgt cacatcgttc gaagctcttt
     1681 gctagaaaaa aataatcatt tcatcatgga cttgtctgac atggagaaaa aaatcgaaga
     1741 aaacaatgta aaagcaatga ttttatgcaa tcctcacaat cctggagggc gtgtttggag
     1801 caaagaagaa ttgaaacagt taagcgaatt gtgtttaaaa catcaagtga ttctgtttag
     1861 tgacgagatc catcaagatt taactttatt cgatcataca tttacttcca tgctaacgat
     1921 tgatcctaat ttagatgatc tactagttgc ttttacttcc gcaaccaaga ctttcaatct
     1981 agctgcaatc aaaaattcaa tggtatttat caaaaatcca gagatgaaag ctgattttga
     2041 aaaacattta gtgatgaacc aacagcacga aatcaacact tttggactga ttggaaccca
     2101 agcggcttat gagacgggtg gagaatggtt aagccaactg attccttatc tagaaaaaaa
     2161 tgttcaaaca gttaatcagt tttttgaaca gtatcttccg aaagtgagaa taatgaaccc
     2221 tgaaggaact tacttaatgt ggttagattt taccgcctat atttcagacg accaagaatt
     2281 ggagaacact cttgttcgga aaggaaaagt cgtattaaat cctggcatca cgtttggtcc
     2341 ttccggtcat gggcatatgc gcttgaatat tgcttgtcca gaagaaactt tactggaagg
     2401 acttaatcga atcaagtatg ctttagagac aatcgattaa atcatttatt caaaataaaa
     2461 ggacgagacc ttactaggtt tcgtcctttt tcataattac tccgtaccat accaaactat
     2521 tctgaaattt caattgtttc aatcactaca tcatgtacag gtcggtcttg tggaccacgt
     2581 tgtacatttg cgatttcatc tactgtgtcc atcccttcta gtacatgtcc aaagactgta
     2641 tgtcggaaat ctagccaagg agttccgcca cctttatacg cttcaatgat ttctgatggg
     2701 aatccagcac cttctagttg tccaagcata ttagctggca catttgtgtt atttacaata
     2761 aagaattggc ttccgtttgt gtttggacct gaatttgcca tagataaagc tccgcgaagg
     2821 ttgaacagtt cacgactgaa ttcatcttca aaactttcgc cataaatact ttcgccaccc
     2881 attcctgttc cagttggatc gccgccttgg atcatgaaat caggaataac gcggtgaaaa
     2941 atcacgccat cataataacc ttttttggaa agttcaatga aattttgaac cgtcttcggt
     3001 gctagttcag ggaataattg aacagtaatg tcaccacggt ttgttttgat aacagctttt
     3061 ggtccttttt cactttttaa atctagttgt gggaatgcca tattgtttcc tcctaatatt
     3121 tgcatacttc ataataataa taacataata cctaacctct cgtaaaaata ctagatttgt
     3181 tttcttttat ttttcatcac tagatacatt ctataactta ccgtttcgat ttcgaattca
     3241 aacatttttg agaagcattc tcaattaaat tctgctatag tgtgaaaagg gaggaatttt
     3301 tatggaaatc tatgacatta caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt
     3361 ttacgcagga atgcgccaag ctaaaacaaa aatcattgac agtctacctc aacttggtgg
     3421 tcaattagca actttgtatc ccgaaaaata tatttacgat attcctgggt atccagcaat
     3481 aaaagcaagt gaattaatcg atcaattaga aaaacaatta actactttta accatacatt
     3541 ccatttaaaa gaagaagttc tctcgcttac tcgtgaagac gaagttatcg agattactac
     3601 aaataaaggc atccattatt ccaaagcagt cattttagct ttaggaaatg gttcattcca
     3661 gccaagaaaa ttgaatttag ataatgctga atcatttgaa aaccacggat tggattattt
     3721 cgtgaatgat ttgatgaagt atgctggaaa aaaagtcgct attgctggag gtggtgattc
     3781 tgctatcgat tggacgttga tgttagagcc tattgcaagc gaagtttact tgattcatcg
     3841 tcgcccggaa tttcgtgcgc acgaacatag tgtatcacgc ttgaaatctt catctgtcaa
     3901 cttgcttact ccctatctca tcgatggatt atcaggcaat aatggggaac tgacagatat
     3961 ccggttaaaa aaagtgaaaa gtgacgagac gattgatttg atgattgatt ctctgatcgt
     4021 caattatgga ttctcttcta atttagaaca tctttcttct tggggacttg atagtaccag
     4081 aaatgccatt actgtaaaat cagatatgtc tacttctatt ccaggcgttt atgcagctgg
     4141 tgacatctgt acctatgaag gaaaagtgaa actgattgct actgggctcg gtgaagcacc
     4201 tactgcagtg aacaacgctt tacactatat taatccaaag gaacgaactc agcctggtca
     4261 cagcacaagc ttatacgata aaaatcttcg tcctagttga ttttttatat ctctttgcat
     4321 ttcaagttaa acccgaaagc agtttcttgc atttttttca tgttccgtta acatgaaata
     4381 gagaaagaaa aaataaaggg ggagcaataa tatggtcgtc aaaactgaga ctttagaaga
     4441 aaaggcaaga gaactattga aggaacgtgg cgtaactgta gaagatatcg cacaacttgt
     4501 catgttttta caaaaagact atatttctga tttgactttg gcggattgta ctgaaagtgt
     4561 caacagcgtg ctaacaaagc gtgaagtaca taacgccatt atcactggaa tccaattaga
     4621 tatattagca gaaaagaaaa aattgatgag tccgttgcaa catattatcg aagatgacga
     4681 aggcctttac ggtatcgatg agatcctagc cttgtcaatc gtgaatgttt acggtacgat
     4741 cggctttacg aattatggtt atatcgacaa ggtgaaacct ggtattttaa aagaactaaa
     4801 cagccacgat ggcgttcatg tccatacctt cttagatgat atcgtcggag cgatcgccgc
     4861 tgctgctgcc agtcgattag cacacaaaga accagaaaaa agaagtcgcc ttactcaata
     4921 attcaacaaa aaaagaaaga aaacgtctat ttagacgagt tctttctttt ttgtttgttt
     4981 caagctattt tttcctaaga aaagaaaaac agcaaaaaat aattctaata atacaaaaaa
     5041 taagataaag ctatgcatga aaaattcttc tggaagtaca ttgatgattg gatcagtaac
     5101 gggatcgaat aaccagtcat cgttgttgaa aaacgtttca tgaaaaagga taaaaaagcg
     5161 atcaaatcct atcatcatga aaaaaccgaa aacaactggc agtaacatgc cgatttgaaa
     5221 cggacgaatc aaccgccaaa gccgatcatt cttttgcaaa tatttgataa acaaaatact
     5281 cgggatcaaa gtgatgaaaa acacagcata atccactaaa aacaaaattt tcacttcata
     5341 aaagtgatgg gctccgctag cagaaactgg gaaatctggc aagctgagga tggttttgaa
     5401 gggattattg agataattca ttaggtgatc aaaatttttc agtaacgtct cttgatctaa
     5461 agaagtataa tccaaaattt gcaaatgtcc gatatcaaat acgtatagtg gtcgaaagtt
     5521 gatcgtcaca ccaactgcca gactgatcag tgtcagaaat aggctgaaca atccagcata
     5581 ttccatccac tgccatctcg aatttttcat attagaagct ccattcatcc agtgattgtt
     5641 tcacataggt agctgggata ggcaacccag gtacatcttc ttcttttgta aaacctgata
     5701 aaacgagcaa agtatctata ttattgcgta tcccagcttg gatatctgtt tcatagttat
     5761 ctccaaccat gattacttct tctttttgca gccctaatac ttcaacggct ttatccataa
     5821 tgatggcttc tggttttccg atgtagatcg gcggcgtttg ggttgctgtc tctacaaaag
     5881 agatgactga ccctgctcca ggaagaaggc cgcgttcagt aggtatattc ttatctgggt
     5941 tcgttccaat gaaagtagca cctttttgaa tagcaagtgt tgctttaact actttttcat
     6001 aggtcagata attatctaag cctaccacta catagtcagg agcctcttct tcccatgtaa
     6061 atccggcagc taaaatcaag tcaatcaatc ctgcttctcc gataacatag acttttttcc
     6121 cttttccatc tgctttcata aaatcgattg ttgccaaagt agctgtgtag actgtctctg
     6181 gagctacatg aatgtcaaac tcatctgcta gacgattagc tactgtttct ggtgacttag
     6241 tcgtgttatt ggtaacaaac aagaaaggta attttctttt ttgcaattct tccacaaatc
     6301 gtttaccggc cggtatgggt tgtgtcccac gataaatcgt tccgtctaaa tcaattaaat
     6361 atcctttata gtccattcat ccactactct tccctttttc tgatcgtaaa tgctgttttt
     6421 gttttttcta cttgtttagg ttgttcttgt ttttttactt tttcagaggt ttttgctgac
     6481 tctgtttttc gatttttaat aaccggtttt ttgcgattag atgcgactgg tccttttttc
     6541 tcatcgatat acgcacgatt atggtttttt ttcttttttc ggcgctgagt tgtcttttct
     6601 tttttaccgc cgattctctc gatgacaaaa tacgcacaac caaaattgca atattcgtaa
     6661 agatagtctt ccaaggaatc gattcgctga tctggatgcg cccgtcgatt atccgcttgg
     6721 aaaaatcctt ttaggcgtag ttgttcataa ccccaatctc ctacgatata atcatatcgg
     6781 gctagtacat cactgtaacg ttctccaagc ttttctgcat taaatccttc acgataatcg
     6841 gacacaagcc gataatgtct ttcacctatc aaaaaattac tgtcatctaa cagtgtcact
     6901 agctcgcctt tgactttttc tgctttaggc tcctcaacga tttcttccaa agcagctgtc
     6961 agttcttcgg tgactgttgt ttcctgttca gctgatgatt ttttcttagc aatttctgtt
     7021 ttatgctcaa cgttctgctg ttcattttgt tttgcagaca tgcgttcacc acctatcttt
     7081 tttattatac cttgttttta tttgattggg tagtgagctt tcaagtaatc tcctaccaca
     7141 cttcggataa attcagggaa caaaaattca ttttttccag caatctcaat cgttggaaaa
     7201 aatggaacaa acatcaaatg atctacggta gtaaaagtat aatttttatt cagatcgatt
     7261 ggcttgttct tccaaaagac ttgatggtta atagaatcat atgtgattcc attatagacc
     7321 atctgcccaa aaatcttacc gcgaaatccc attcccttca taggatagtt acgtaaaaag
     7381 ttacgatttt tttctatttc taaaactaag cggatcagat catttccacc caatgtgaca
     7441 tttaacaagt gcatcggatg cggcaacgca gtgtgaagct gatcctgatt gacccgccct
     7501 tttggaattt cagttaaaaa cagaccactg tttaatattg ctgcttccgt ctttcctcgt
     7561 tctttcaccg cctcaaaggc tacttggata aacgaatgct cattgaagat atctagactc
     7621 agagaaaatg gcaaatctgc tacttctttt tcttttaata attcatgccc acgttctaga
     7681 taactattta tttcttgttc atcttctgta aaagctgtca ttgtttcggt agggatggct
     7741 ttggctgaat gcttaaggat ttttttctct tcatcgatgg ttaaatggac ttcaccgaca
     7801 tactgcccgt actttccagc ggctgctatt tggacatcat tgattacgtg accttctctg
     7861 aataaatgat gggtgtgcga tcctaaaata acatcaattg ctggtatttc ttttgcaatt
     7921 tggaaatcat cctgaatccc gagatgactc atcaaaacta aaacatccac ttgtgaacgt
     7981 aaatcttcga ctaattctgg caaaatatca taaggattcc gtatgtccca gccattcgga
     8041 ttataagtaa gcggaaatgg agcagtaaat gcaattagtc cgattttcgt ctggtgtttc
     8101 gttgtaatga ttttatatgg tttcgtccat gccggtggct ggagcgtatt tttatcgaat
     8161 aagttatcca ggatgatatc aaactcagca tgatcgtata aatgattcaa ctcttctttg
     8221 gaatttccta cgccttcatt gtttcctatc gtcgctgcat cataaccaac ttcattcatc
     8281 agcaagatat tttcctgtcc attcgttgct tctgacaatg gatgccaacg atccacaaaa
     8341 tcacctagat ctactgcaat cgttgtcgtt ttttctttct tttcgttttc tcgtttacgt
     8401 tgatccaaaa accttctgat ccttggccaa ttttctaaat gagaatgcag atcattcgta
     8461 tgtaaaatca ctattttttc catgtaccct ctcgcctctc tttatcttta tcataccata
     8521 ggcagctttt tcttccattg aaacaagctc tatttcagag tttttcctgc cttgaagcaa
     8581 attgtataag tacaatattc gataaatcag tctggctaaa aaatcaggaa cattcatttt
     8641 tacagtacta taaaaataaa aaaaacagac ccgaaagtct gttgtcgttt cattataatc
     8701 agacacatgg cggaacctgc ttagtgctgc ttccttccag acctgacacg cttcacaagc
     8761 ccaccattgt cctagccttt cggcaaagaa tagtatacct tatttttcat tcgttgacca
     8821 gtcttttttt aatttctttt ttcgctggcg gagctttcgg aaaaagtcag tcaaaatcat
     8881 cccacattcg tcttctaaaa tgccaccttc tacatatgct tggtggttaa accgctcgtc
     8941 ctccaaaaga ttcatcaagg taccggctgt tccgcctttt ggatcatacg cgccgaaata
     9001 aacttctgct acacgagaca aaatcatcgc gccgctgcac ataggacaag gctctaaggt
     9061 aacaaaaagt gcagcatctt ccaagcgcca gcttcccact ttttcacagg cttccttgat
     9121 tgcaagcatt tctgcatgtg tagtagcatc ttgcgtggtt tctctaaggt tatatcctct
     9181 tccaatcact tttccatcca atacaaccac tgctccaatc ggaacttcat gaagcatttc
     9241 tgctttcttt gcttctgcca atgcaaggcg catccatttt tctttttcta ctgcatcaag
     9301 atttgtattt accaattacc cttccctgcc tttgtttttt cttgttttac tttagcatat
     9361 gataaaaagg ctgtaactaa cttttttttc tatggtaaaa taaatacaag aggtgaacaa
     9421 gatgaaaatg gataaattac ttgctcttta cccacaagca cagaaaaaaa actctccagc
     9481 atcagaagcg gatatccttt cttttgcaat tgatgatgct tttatctgga tcaaacaaga
     9541 ttcattatct caacaagaaa ccagcctttt gaaagcgtta ttccctgtaa tcaacgatca
     9601 aaaaaaacac ccatggtatc gctatctttt taaagatgct tcttgtgttc aagaaaaaag
     9661 ctttcgtttg atccagcttc acgtcgaatc aaaaggagaa ttcttaaaaa aagaatggca
     9721 agatactatt ttggaattat ttcgctattc tgctgatttc tttttttata cagacacaga
     9781 tgcgctctta gtcgaacaaa taaaccgtga ttatcttgat gctactgaaa tcaacggtat
     9841 ttttctatct ttagatgctg attttgatac gacaacgtct gtattcgttg gttctttcca
     9901 ttcacctggc accgacctcg tccctttgtt tgctgaagaa agaaaaattt ttttaacaga
     9961 gcaaaacaat ctttatactc acagccgtac tttttctatg caagatgtgg ctttacatta
    10021 ctatacaaaa gatactgtaa aagatagtaa gttgatcaaa agttacatag aactgatcaa
    10081 acaaaatgaa atggaagtga tcattcaaga attatggaat gaattaggca atgtcagctc
    10141 tgctgcaaaa acactgtttc ttcatcgtaa tactctaaaa tatcgaatcg aaaaatttca
    10201 agaacagtct ggtttcaatc taaaaaaagc caacgattta ctgttttgct atcttttatt
    10261 gcttcatgaa cataattaaa gatagattaa aggggttgtg acaataattt tgtcacaacc
    10321 ccttatttcg aatgaatagt gttcaaaagc tagtttctct tacatcattt tttcaacaaa
    10381 atgcattagc cggtctttta catcttcggt tgaatctttg attgaatcac cataaacaga
    10441 gattttcttt tttcctgatt tgatttttgc taacattccc atcaaagaat ccaagtcact
    10501 atctgaaaga ccatctacga tttctagtaa tttttcattt ccatcaaatt tgtcatagac
    10561 gaattttttt actttcgtcc gagtagtcat atgatggatc ttttccatta cttttcccga
    10621 tacaacgact gctgtcgcta cactggcagc ggctgctacg cttaatccga ttgttacttt
    10681 tgttgatgtt ttcataagta tcagctcctt tttatctatc tccatcatag catagacaaa
    10741 aaaataaacg aactaatcca ctcagtgaaa tctcaatcct ttagggaaaa agttctttac
    10801 tgttcctttt acttgtttcc caaatccgac aggtatctta tctaatacta gcaaaaccca
    10861 tccttgattc ccatcacgtt gaaatgtttc tcctgctgtg taagattgcc attccttttg
    10921 agtgatcggc aagcaaggta tattctctat cttttttgta gcaagcgcta atgcatagct
    10981 aggttcaaat cgattctttt tgaaatctcc taaatgcaaa cctgtacgta cgacttttaa
    11041 accatcgaga gaaggggcta actcaggaac ttcccaaaga tggtcgttga acactaaaag
    11101 tcttccagta gcttcataat gaaaatcatt tgaaaactcc gtccagagtt tttcctgctc
    11161 ttttgtcatt tgcactttac ttttttttcg tgtcttcttt tctttatgca tctgattttg
    11221 tccatgaaaa gtcagctttg caacaaaatg tccttcaccc tgatctttat gaggccatat
    11281 acgtattgtt ttttccaaac cagctacgct tccccattca gaacgtcctg aactaacgga
    11341 ttgagtcaaa ggaatctcct caatcgttac tggataattt tctacaagcc agctaatgat
    11401 ctcttcattt tcttctggtg caaacgtaca agtcgaataa atcagttgtc ctttattttt
    11461 tagcattttt attgctgaag acaaaatctc ttgctgacgt ttttgacagt atagcggaga
    11521 ttcttctgtc cactctttta ttgcatttgg atcttttcgg aacattcctt ctcctgaaca
    11581 aggagcatca accacgatcc gatcaaaaaa gcccgaaaaa tgagggacaa gttctgcggg
    11641 tgcatgattt gtcactattg cgttcgaaac tccccatcgt tctatatttt ctgataaaat
    11701 cttcgcacgt ttcggaaaga tctcattcgt cactaataac cctttcccct tcatttgagc
    11761 agctaattgg gttgatttac caccgggagc cgcacacaaa tccaaaactt tttcaccggg
    11821 ttttgctgca gcagctgttc caacgatcat tgcacttggt tcttggctat attcataccc
    11881 agcttgatgg aggaaagact ttccattgac agtgccaaga aatccctcat tgctgtaggg
    11941 agcgggttgt aattcttcgc tatggtaagt ttggaccatg tccaatcctg ccggttttag
    12001 tggattccac cgaaatcctt ttttcacgct tccttgttca agcgcagaaa aaaaatcgga
    12061 tgcttcttcg cctaaaagaa gacgatattt tttgataaat tgctgtggta acgttgtcgc
    12121 ttcctccttc attccaatgt ttcgttagac attttacaat cttttttacg aaaaataaag
    12181 gtggctgatc tactttttca tgttgctgcc taaggtgact gttcctttaa gatattttcc
    12241 aagtattcat ccgtcacctc gtttttatcc caaacagcgt tcatcatttc aagaaaatct
    12301 tgagaagaat catctgagcg caaacttccc aatgcattaa gccataccaa ttcaagcgac
    12361 ttttcttctt tgatcttcgt gtgacctttg acatatgaga aaaatggccg cttaaatagc
    12421 gaatccatgt ctaaatcaga ttttgcaatg atttcacgaa tactttcatg gatagaaaaa
    12481 aaatctttca ataagtagac aatgcttaaa acatcatcgg aactgcattc agctaagtag
    12541 ttttcaaaat aaacagtgat ttcttctaat atcttctctt ttaacccgct gattgatttg
    12601 aaatgtgtat aaatcgggta tgtagaacaa ccgcagaact ctgctaactc tcttgcagta
    12661 attgacttca ttccatattc ttccacgtat accatacttt tgaacagcaa atattcttta
    12721 ctataatttg ttactctccc catcattctt acctccgtct cttttcatct acccataaat
    12781 aaataacata aataattcta aaaatattat tgataattat caaaacatat ttagttatat
    12841 ccattaaaaa tcaatttttc cgccttaaaa acaagaatat cttgacaaaa taccctagga
    12901 aataaacaat agaataaaca aggtaacaaa aaaattaaat aacgttatat aaaaaagaag
    12961 gcgacactct gtgagaataa cacagactga aatgactttc cactcacagt ttttgtctaa
    13021 ctatatacaa aaacagaata aaaaggaggt atttatgcta attgaagcaa gaaaaaaaga
    13081 gttacttgtt ctacaaatgg tcattcaaaa aaataattgt tctttacaag aattatccaa
    13141 tgacttacat attccaaaac ggacaatcaa agaactgatc cgaaaaataa atgtatcact
    13201 caagcaattt ttattgattg atgaatttat ttattctaat cataaaggag agatccaaac
    13261 caaagaaacc gatcaaacta aaaagttagc tgttttttct aagttaaaat tatgctacct
    13321 aaaagaatcc aatcgtttta actatttgat gttactaatc aattatccac aaactgctgt
    13381 accaaaaaaa tatttacttg aacaacttta tatatcgccc tcctatctcg aaaaactgac
    13441 aaaacagctc aatcattcct taaaaaaatt ccatttacgg atcctatctg taaaaaattg
    13501 ctgtactctt gaaggaaatg agttatctat ccgtctctat ctttactttt ttctttcaga
    13561 tacatttgga ggtaacgatt ggccattttc ctcacttgaa ttaaatgcac taaaaatgaa
    13621 cagtacagat gagcaaatag aaaaaatttc aaaacaagaa aaaaatatct acttgctgtt
    13681 aactttattc atattacgaa acaaacaaca ggcatatttt ccaaaaactt cttctgaaat
    13741 cactgaattg atggacttat tgcagcatac acatgattat tcagaatact ttacttcttt
    13801 ttcagtgagt cgcccttgtg aagagacaga aacagagaga gcctacttta attatttttt
    13861 ctattgtttt tttccaaatc aaatctcttt tcgacaaaaa caggagattg gttttttatt
    13921 ttccacaagc aaaaacctta tttgtcgaca agtcggtcaa ttgatgactt acttaactca
    13981 acagtttcct ctcaaccatt tagaaaataa aataccaatt tatttttatt ttcttgtgtt
    14041 ggcagttgtt tttttcgaac tgttgggtga atccagcagt agtttttcta cactttgctt
    14101 tccagaagcc acgtccaatc attctagttc ttttatacag gaaaagaaat taagagaaaa
    14161 aatcgataca attttttcaa aaaatgggga aagaaaccaa atcagtcaat accttagtca
    14221 attgatccaa cacttactac aaacagaaac gaaagagcag ttactcatcc atttgcagat
    14281 aaccaaacag tttacaggaa attatcatct tcaaagccat ttgcagcagt tatttcgcaa
    14341 agatgctgtt aaaattaccg catattcaga acatgctgat ttgatcatta cagatcagca
    14401 tgagaaaatt tcagatgaaa cggagacttt ctatctggat acaacgcaca atacagtgtt
    14461 atttggcaag ttaataaaag cggttcatga actgtatatg aaaaaaggtt atgagaacaa
    14521 aacaaagagg atgtgacaaa attcttgtca catcctctta tt
//
LOCUS       DARBDV010000073        14342 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000073 DARBDV010000000
VERSION     DARBDV010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.073"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            108..489
                     /locus_tag="SCM56_002119"
                     /pseudo
     CDS             108..489
                     /locus_tag="SCM56_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303242.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            620..1570
                     /locus_tag="SCM56_002120"
     CDS             620..1570
                     /locus_tag="SCM56_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002821555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEG1110871.1"
                     /translation="MLKTYSLTKDTLLVSSESNIKNSSVVYVYGDEREYIESSEHKYD
                     FKIGNILTFDDRVAYDTMIDKNDEKSLLVSFLFPEVHEGGHLDTLTTSVVFLVFKNKL
                     IIFTKQKLKFIPELLSNMVLRDVNHFVEEVLLKIMQKDFYMMLNDLRGVKEKIDDLDS
                     EISTSGSLKPTFGDLLTLQKYMIALSTTYKANHKALDFIKKNFTTIDDIDFSTKKIID
                     DLYDTSDTMDRIILSYSQYLDKLENMINNMISYQLNMIMKTLTEISIVLTIPAIIFGF
                     WGINVHVPFEKSSYGVLAVLIISAILSLICWLLMRRKTYL"
     gene            2088..2543
                     /locus_tag="SCM56_002121"
     CDS             2088..2543
                     /locus_tag="SCM56_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1110872.1"
                     /translation="MVQQIVLPIKDSSVLKMVQDTLLDSFRAGRRNYTVFQVGKATLL
                     RVSDVMTLKKSDVYNPDGSVKNATFIHDKKTGKANTLYLKPVQQDLLQYHDWLAQENI
                     NSEWLFPSTAHYDLHITEKQFHKVMAHVGDLLGINLEDKEKKLRVRKPF"
     gene            2941..3486
                     /locus_tag="SCM56_002122"
     CDS             2941..3486
                     /locus_tag="SCM56_002122"
                     /inference="COORDINATES: protein motif:HMM:NF013878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110873.1"
                     /translation="MELAEPGHYDEKWQNWKLESLPIFPDRYDFEVAKDKGKQFKIVA
                     ELLKKANTIIVATDSDREGENIAWSIIHNANAFSKDKTFKRLWINSLEKDVIRSGFQN
                     LQPGMNYYPFYQEAQTRQIADWLIGMNASPLYTLNLQQKGVQGTFSLGRVQTPTLYLI
                     FQRQEAIENFKKEPFFEVEAK"
     gene            3580..3912
                     /locus_tag="SCM56_002123"
     CDS             3580..3912
                     /locus_tag="SCM56_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase I"
                     /protein_id="HEG1110874.1"
                     /translation="MGNQEGIIADVQTKEKKTNSPSSFSLSSLQSKVNQLYKATASQT
                     LKAMQGLYEAKLLSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMLLSLIMIRSN
                     NSTQILTN"
     gene            complement(4063..5042)
                     /locus_tag="SCM56_002124"
                     /pseudo
     CDS             complement(4063..5042)
                     /locus_tag="SCM56_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332307.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(5047..5814)
                     /locus_tag="SCM56_002125"
     CDS             complement(5047..5814)
                     /locus_tag="SCM56_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEG1110875.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            6304..6729
                     /gene="lacA"
                     /locus_tag="SCM56_002126"
     CDS             6304..6729
                     /gene="lacA"
                     /locus_tag="SCM56_002126"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEG1110876.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            6746..7261
                     /gene="lacB"
                     /locus_tag="SCM56_002127"
     CDS             6746..7261
                     /gene="lacB"
                     /locus_tag="SCM56_002127"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEG1110877.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            7272..8204
                     /gene="lacC"
                     /locus_tag="SCM56_002128"
     CDS             7272..8204
                     /gene="lacC"
                     /locus_tag="SCM56_002128"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEG1110878.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            8207..9187
                     /gene="lacD"
                     /locus_tag="SCM56_002129"
     CDS             8207..9187
                     /gene="lacD"
                     /locus_tag="SCM56_002129"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEG1110879.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            9215..9532
                     /locus_tag="SCM56_002130"
     CDS             9215..9532
                     /locus_tag="SCM56_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEG1110880.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            9532..11238
                     /locus_tag="SCM56_002131"
     CDS             9532..11238
                     /locus_tag="SCM56_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1110881.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            11380..12786
                     /gene="lacG"
                     /locus_tag="SCM56_002132"
     CDS             11380..12786
                     /gene="lacG"
                     /locus_tag="SCM56_002132"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEG1110882.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            12809..13081
                     /locus_tag="SCM56_002133"
     CDS             12809..13081
                     /locus_tag="SCM56_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HEG1110883.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            13102..14001
                     /locus_tag="SCM56_002134"
     CDS             13102..14001
                     /locus_tag="SCM56_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEG1110884.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         4685 a         2363 c         2765 g         4529 t
ORIGIN      
        1 gacactatcg ttcttcatca acgtaagtta gatttgttga aagagcagaa aaaaggattt
       61 ctacaaaaaa tgtttgtttg atattttaaa tagaaaagag agataaagtg aagtttttaa
      121 attttttgaa ctataaatat ttaaactggg tattatatgg tattacatta ttttttatta
      181 tttattactt ttttaccgat gatgatattt ttttaatttt agcaatactt tttcttgttt
      241 catctaattt tagtgataac ttaattgaag ttcttacaga accagaaatt cggtctatga
      301 cggatgaaga acaacagatt attaaagcta tgatttcgga aaataagaaa gacgtagtag
      361 ttatcaaaaa gattagagag tatacaaatt taggtgtttt acaagccaaa aaagtatatg
      421 aaaaaaaaaa aaagggtcta taattacaaa ttagataata cattagaagc tggagacaag
      481 attgtctaga taatgtgtga gataaaattt ttggcaggtt atcatacata agacaaaatg
      541 tttttaaaac tgataacgta tttattccac aacaattacg catttatttc tgagaaaaat
      601 ataaagcagg aggattacaa tgctgaaaac ttattcctta acaaaagata cattattagt
      661 ttcgtcagaa tcaaatatca aaaattcttc tgttgtttat gtttatggtg acgaacgaga
      721 atatatcgaa tcttctgaac ataaatatga ctttaaaata ggcaatatac taacgtttga
      781 tgatcgagtt gcatatgaca cgatgataga taagaacgat gaaaaaagtt tgctcgtaag
      841 ttttttgttt cccgaggtac atgaaggtgg gcatctagat accctgacga catctgttgt
      901 tttcttggta tttaaaaata agttgataat ttttacaaaa caaaaattaa aattcattcc
      961 cgaactctta tccaatatgg ttttacgtga tgtcaatcat ttcgtggagg aagtactttt
     1021 aaagatcatg caaaaagatt tctatatgat gcttaacgac cttcgtggag tcaaggaaaa
     1081 aattgatgat ttagactcag aaatatcaac gtccggttca ctcaaaccaa cgtttggtga
     1141 tttattgact cttcaaaaat acatgatagc tttgtcaaca acttataaag ctaaccataa
     1201 agcgcttgat ttcattaaaa aaaatttcac aactattgac gatattgatt ttagtactaa
     1261 aaaaataatt gatgatttat acgatacaag tgatacaatg gatagaatca ttttaagtta
     1321 cagccaatat ttggacaaat tggaaaatat gataaacaat atgatttcgt atcagctaaa
     1381 catgattatg aaaaccctaa ccgagatatc aattgttttg actattccag caattatttt
     1441 tggcttttgg ggaatcaatg tccatgtccc gtttgaaaag tcttcttatg gtgttttagc
     1501 cgtattgatt atctctgcta tactaagtct aatttgctgg cttttgatgc gacgcaaaac
     1561 atatttgtaa agtatttgtg ataccctaat atgatttaaa gcagaatttc tttgttgact
     1621 ttttaaaagg ctgattcctt tcaataattt aatagcttca gcggctgctc agaagtcaat
     1681 aaattggact atttttattc agttacttag gcaacatttg agatacgatt gcgattataa
     1741 aaattgatat accaaccaat ggctgcttgg aagactgaca tattagcctc gatatcatcg
     1801 aataactaaa tacagaaaaa tacaataaat attatcaaac tagggactat actagaaaac
     1861 atcagtacat tccttaactc agggtctata attagaaaat cgcccctttg aaaactgatt
     1921 aaaacagccc ccattatcta gtgactagat aatgggggct gtttttttgt taagtgatat
     1981 aataaaccat aaaagaagtt tagaaatacg gtcctggtca tcaataaata aacaatttaa
     2041 ttaattattt atttaattat ttaatttcag aaaggtatac atgaatcatg gtgcaacaaa
     2101 ttgtcctacc catcaaagac tccagcgtct taaagatggt acaagataca cttctcgata
     2161 gtttccgtgc tggtcgccga aattacaccg tttttcaagt tggtaaggcc acgctactac
     2221 gtgtgagtga tgtcatgaca ttaaaaaaat cagatgtcta taatccagac ggctctgtca
     2281 aaaatgcaac ctttattcat gataaaaaga ccggtaaagc aaacacgtta tatttaaagc
     2341 ctgttcagca agacttgttg caatatcatg attggctggc ccaagaaaat atcaattctg
     2401 agtggttatt tccctcgaca gcccattatg atcttcatat cactgagaag cagtttcata
     2461 aggtgatggc gcatgttggt gatctcttag gaatcaactt ggaagacaaa gagaaaaaat
     2521 tgcgagtcag aaaaccgttt tgagatatga acagcatttg ccttcacgcc ttagcgattt
     2581 taaataatag ccttgaaagt gattatggaa aagccctcta cgctcaatgc aaaattgacg
     2641 tagaaactat ttttgatcga ttgaaaggtg tatttggtat gtgaagaacc catgtcagag
     2701 gcaagaacgt tgcacctact gacattggaa tgatgcttat gagcatgaat ctaaccaaat
     2761 tatcccttga ggctagaaga aaagcggaag cttttcaaca caaagcagcc aaaaataaaa
     2821 aacgcgacga agcaatcaca tttatgatta ttcatcgcgt tttttatatt tagagctagt
     2881 tatttgctag acccactatt tacagatgaa acgtttatca cctttggttt tgggcattta
     2941 gtggaattag cagaaccagg tcattatgac gaaaagtggc aaaattggaa acttgaatct
     3001 ttgccgattt ttcctgatcg atacgatttt gaagttgcaa aagataaggg aaagcagttt
     3061 aaaattgttg cagaacttct caaaaaggca aatacaatta ttgttgcaac agatagcgac
     3121 agagaaggtg aaaatatcgc ctggtcgatt atccataatg caaatgcctt ttcaaaagat
     3181 aaaacattta aaagactatg gatcaatagc ttagaaaaag atgtgatccg aagcggtttt
     3241 caaaatttgc aaccaggaat gaattactat cccttttatc aagaagcgca aacacgccaa
     3301 attgccgatt ggttgatcgg catgaacgca agccctttgt atacgttaaa tttacaacag
     3361 aagggcgtac aaggtacatt ttcactagga cgtgttcaaa cgcccacctt ataccttatt
     3421 tttcagcgcc aggaagccat agagaatttt aaaaaagaac cttttttcga ggtggaagct
     3481 aagtaaaaca agggtcattt aagggcgttc taagccccac acagcgtttt aaaacccaag
     3541 aggagctttt tagcttttgt ttcttctaaa caagctaaaa taggcaatca agaggggata
     3601 attgctgatg ttcaaaccaa agagaagaaa acgaatagtc cgagttcgtt ttctttaagt
     3661 agtttgcaat caaaagtcaa tcagctttat aaagcgacag cgagccaaac tttaaaagct
     3721 atgcaaggac tgtatgaagc aaaattattg agttatccaa gaacagatac accatttatt
     3781 acagagaacg aatttgctta tttaaaagcg aattttggca aatatagcgg ttttttagga
     3841 cttgatcttg aaatgttgtt atcattgata atgatacgtt caaacaacag cacccaaatt
     3901 ttgacgaatt agtgaaactc tatgaaaaag acgtagtaaa acatgcaaca tcctattcta
     3961 atcagctggt aaagcttaat taggttatat aagcttgaaa ataaagggtt tgtccaatat
     4021 ggacaagcct ttttcttatg ttttgccaca gatttgccac acttattgtg taagagtatc
     4081 aaggataggt ataatttgat actttgtttt tttagtgatg tgcaaataaa tttctgttac
     4141 acgactacct ctagaatgcc ctacacggtc ttgaattgct tctaggggta ttccttactc
     4201 tgctaagaaa ctaatatgcg tatgacggaa tatatgggtc gtaatatgtt tattaaaact
     4261 aagtttatta tcaatatcaa ttcctgctcg ttttggatta ccttttcgat ttaattttcc
     4321 attttcaggt gagttgagga aattgttaag gacactataa tgaagaggtg ttccattata
     4381 gtcgggttta tgacgacttt tggaagtaaa aatccatcca ttatctttat aatcaggatt
     4441 gaatctcata tgttggtgat ttaactcaat ttcctcttta agaatttcta tagccctttt
     4501 agataattgg acatttcggt aactttgtaa gttttttaca ttaccttcat agctattttc
     4561 tgcagctcct ccgtgagtgg actgactaat gatgatattt tgcttttcaa aatattgata
     4621 ggcaccaata ttttggagtt tcgactctgt aagtcaacag ggccattagt tagaatacct
     4681 attgggatgc ggtttttatg tagagccagc agtagttgat gtatctcagg aatcatcgta
     4741 atctgatcga gttcatattc atagtcagct tggaagtcta gagcttctgt cctagaaata
     4801 ctatcgatgc caaattcttc catagtcttc tgacagcgaa aaattctaag ctgctcaata
     4861 ctgatgagtt ttttcgtgta tttaggaaaa gcaacatcag accaatatct gaaacgtttg
     4921 taaatgttat ctatttgttg gatatcaatc gttggaaaac atttctcaac gctacgttta
     4981 aaggggagtt gatggtcata aagagtgtca tcaaggtcaa aaacaatatt tttataagtc
     5041 ataattctag tccgctttta aaatagtagt gtactggcta aattcctcta agtgttgagg
     5101 gctaatctgg ttatctgtaa tgatggtatc gagttgatct agattataga agttaaagaa
     5161 atcgtatcga tcgaatttag tactgtctac taataagaat ttttctacag cattgtttag
     5221 ggcaagttgt tgaatcacac cttccttgtc actatatgta gcaatagaat tatgggtaac
     5281 agcatttgca ctaacaaaag ctttggcaaa tctaattgct tttaaatttg tcgaagccat
     5341 tgaacctaca aaagctccag ttatttctct atattcaccg ccaagaagca ataaatcaat
     5401 ggtttcgcta tcatttagaa tcaagaacac agggagacta tttgtaatga cacgaatttt
     5461 ataaccttta cgacctttca attctaatgc cagttgttca agtgtagttc caggtccaat
     5521 aaaaatagta tcgccatctt tgattaaaga gcaggctttt ttagcaatgt caattttttc
     5581 ttttgtattt agacttttct tctcgatatg tgtcttttca agtggctttt tagaggaaag
     5641 caattgtgca ccaccatgag tacgtgttaa aagtccatca gcttctagag catcaaggtc
     5701 tctacgggct gtcatatcgg aaatatctag ttcatctatt atttctttta tggttatggt
     5761 cccatcaatc tttaacatat ctaaaatttt ttctaatcgt cgttttttgt tcatatgaag
     5821 acccttcttt cataaagtaa tttttttcca aagataattc tcttttaatt gtatcataaa
     5881 agacaatatt ttcaaggtaa aacaaacaat ttcaaacaaa aacaaatgtt agatgatgaa
     5941 ataagaacag aggattgacg tatattagct taggtcagat tttgtataag acgaaaataa
     6001 agtaggacct cttaatcagt aagttataga aagtaaaaga cttttgtaat acttgaatag
     6061 atatttcacg tccattttgt gatggattaa atgaacaaaa atgaacaata atttaacggt
     6121 gttatctatt ttttaaaaaa acaaataaaa aaaacaaaaa ataaacaaaa aaagtgttgc
     6181 tttttgtttg aatgcttgat attatataaa caaagaaatg atgaaaacgt tttttcgaac
     6241 attttgcaaa atgttttcta cttctacgta gcatttcttt ctaaaattta ggaggtggtc
     6301 aaaatggcta ttgttgttgg tgcagatctc aaaggtacga gattaaaaga tgttgtgaaa
     6361 aattttcttg tagaagaagg ttttgaggta attgatgtga ctaaggacgg acaagatttt
     6421 gttgatgtta ccttggcagt tgcctctgaa gtaaataaag atgagcaaaa cttaggtatt
     6481 gtaattgatg cttatggagc tggtccattc atggtagcaa ctaaaattaa aggtatggtt
     6541 gcagcagaag tttcagatga acgctcagct tatatgacac gtggacataa caattcacga
     6601 atgattacgg ttggcgctga aatcgtcggt gatgagcttg ctaaaaacat cgctaaaggt
     6661 ttcgtcaatg gtaaatatga tggcggacgt catcaaatcc gagtagatat gcttaacaaa
     6721 atgtgttaag aaaaggagtt aaggaatgag aatcgcaatt ggatgtgacc acattgtcac
     6781 agatgaaaaa atggcagtat cagaattctt gaaatcaaaa ggacatgagg tccttgattt
     6841 cgggacatat gaccacgcac gtactcacta ccctatctat ggtaaaaaag tcggtgaagc
     6901 agtagtaagt ggtcaagcag acttgggagt atgtatctgt ggtacaggtg tcggcattaa
     6961 caatgcagtc aataaagttc caggtgttcg ctcagcgttg gttcgtgata tgacatcagc
     7021 tatatatgca aaagaagagt taaatgcgaa tgttatcggt ttcggtggaa agattactgg
     7081 tggtcttctt atgaatgaca tcattgaagc tttcattgaa gctgagtaca aaccaacaga
     7141 agaaaataaa aaattgattg caaaaatcga acacgttgaa acacataatg cacatcaagc
     7201 agatgaagat ttctttacag aattccttga aagatgggac cgtggtgact accacgatta
     7261 aggtataaca aatgattctg acagtcacac taaatccttc agtagacatc tcttatcctc
     7321 tagaaacatt gaaaattgat acagtgaata gggtgaaaga tgttagtaag acagctggtg
     7381 gaaaaggctt aaatgttact agagtcttgt atgaatctgg tgataaagtt actgctacag
     7441 gtttcttagg tggtaaaatt ggtgaattta ttgaaagcga attagaacaa tctcctgtta
     7501 gtccagcttt ttataaaatc tcaggaaaca ctagaaactg tattgctatc ttacacgagg
     7561 gtaaccaaac agaaattttg gaacaaggac caacaatttc tcatgaagaa gcagaaggct
     7621 ttcttgacca ctatagcaat cttattaaac aatcagaagt tgtgacaatt tcaggaagtt
     7681 taccttcagg acttcccaac gattattatg aaaaacttat ccaacttgct tcggatgaag
     7741 gagtagcagt cgttttggat tgttcaggtg cacctcttga aacagttcta aaatcgtcgg
     7801 ctaaacctac tgccatcaaa cctaataatg aggaactttc acagttgctt ggaaaagaag
     7861 taacaaaaga tattgaagaa ctcaaagacg ttcttaaaga gtcacttttt agtggtatcg
     7921 aatggatagt ggtctcatta ggtcgaaacg gtgcctttgc aaaacatggt gatgttttct
     7981 ataaggtaga tattcctgat atcccagttg ttaatccagt cggatcaggg gactcaacgg
     8041 tagctggtat tgcatcagct ttaaatagta aaaaaagtga cgctgactta ttaaaacatg
     8101 cgatgacatt gggtatgtta aatgctcaag aaacaatgac agggcatgtg aatatgacta
     8161 attacgaaac actaaactca caaattggag taaaagaggt ataaaaatgg tacttacaga
     8221 acagaaacgc aaatctttgg aaaaaatttc agataaaaac ggtgttatct cagctttggc
     8281 atttgaccaa cgtggtgctt tgaaacgttt gatggcacag tatcaagata cagaaccaac
     8341 tgtagctcaa atggaagaac taaaggtttt ggttgctgat gaacttacaa aatacgcttc
     8401 ttcaatgttg cttgaccctg aatatggact tccagcaaca aaagcattgg ataaagaagc
     8461 aggtcttctt cttgcctatg aaaaaacagg ttatgataca tcaagtacaa aacgtttgcc
     8521 tgactgcttg gatgtttggt ctgcaaaacg tatcaaagag caaggcgctg atgccgttaa
     8581 gttcttgctt tactatgatg tagacagctc agatgaactt aaccaacaaa aacaagctta
     8641 tattgaacgt gttggttctg aatgtgtcgc tgaagatatt cctttcttct tggaaatcct
     8701 tgcttatgat gaagaaatct cagatgcagg ttctgttgaa tatgcaaaag tgaaacctcg
     8761 caaagtcatt gaagcgatga aagttttctc tgatccacgc tttaacattg atgttcttaa
     8821 agtagaagta ccagttaacg ttaaatatgt cgaaggtttt gctgatggtg aagtagttta
     8881 cagtaaagct gaagctgctg acttctttaa agcacaagaa gaagcgacta accttccata
     8941 catctaccta agtgcaggtg tatctgctaa attgttccaa gaaacattgc aatttgctca
     9001 cgactcaggt gccaagttta atggtgtgct ttgtggacgt gctacttggg caggatctgt
     9061 agaaccttac atcaaagaag gtgaaaaagc agcacgtgaa tggttgcgta ctactggatt
     9121 cgaaaatatt gatgaactta acaaagttct tgttaaaaca gctagtccat ggactgataa
     9181 agtatagtaa aaacataaaa cggaggatat tgttatgaac agagaagaga tgactctctt
     9241 agggtttgaa attgttgctt atgctggaga tgctcgctct aaacttttag aagcgcttaa
     9301 agcggctgaa aatggtgatt tcgctaaggc agatagtctt gtagtagaag caggaagctg
     9361 tattgcagag gctcacaatt ctcagacagc tatgttggct cgagaagctt ctggggagga
     9421 acttccatac agcgttacca tgatgcatgg tcaggaccac ttaatgacta ctgtcttatt
     9481 aaaagatgtg attcatcacc tcatcgaact ttataaaaga ggagcaaaat aatgcataaa
     9541 ctcattgaac ttattgagaa agggaaaccg ttctttgaga aaatctctcg aaacatctat
     9601 cttcgtgcta ttcgtgatgg atttatcgct ggtatgccag tcatcctttt ctcatctatc
     9661 tttatcctta ttgcctatgt cccaaatgct tggggattcc actggtcaaa agacattgag
     9721 accttcttga tgactcctta tagctattcg atgggtatcc tagcattctt tgttggtggt
     9781 actaccgcta aagctttgac ggactcaatg aaccgtgacc tacctgcgac caatcagatt
     9841 aacttcttat ctacgatgct agcgtctatg gtagggttcc ttttgatggc agctgagcct
     9901 gcaaaagaag gaggcttctt gacagccttc acgggaacaa aaggtctttt gacagcattt
     9961 attgcagcct ttgtgacagt taatgtttac aaggtctgtg tgaaaaataa tgttactatt
    10021 cgtatgcctg aagaggttcc accaaatatc tctcaagtat ttaaagactt gattccattc
    10081 actgtagcgg ttgttctcct ttatggattt gaactcattg ttaagggaac tcttggtgta
    10141 accgttgcag aatcaatcgg tacccttctt gctcctctct tctcagctgc agatggttac
    10201 cttggaatta cacttatctt tggtgcttat gccttcttct ggtttgttgg tatccacggt
    10261 ccttcaattg tcgaaccagc aatcgctgct atcacttatg ccaatatcga tgccaacttg
    10321 catcttatcc aagctggaca acatgcagac aaagttatca cttctggtac tcaaatgttt
    10381 attgttacca tgggtggaac aggagctaca ttgattgttc cattcttgtt catgtggatt
    10441 tgtaaatcag aacgtaaccg tgccatcgga cgtgcctcag ttgttccaac attctttggg
    10501 gttaatgagc caatcttatt tggtgcacca atcgtattga atccgatttt ctttgtaccg
    10561 ttcattttcg ctccgattgt caacgtttgg atctttaaat tctttgttga cacattgaac
    10621 atgaactcat tctctgccaa ccttccatgg gttactcctg gtccgttggg aattgtactc
    10681 ggtactaact tccaagttct atcatttatc ttagccggac tcttggtagt tgttgatact
    10741 atcatttatt acccatttgt taaggtatac gatgaacaaa ttcttgaaga agaacgttct
    10801 ggtaaaacaa acgatgctct taaagaaaaa gtagctgcaa acttcaatac tgctaaagca
    10861 gatgccgttc ttggaaaagc aggcgttgct aaagaagatg tagcagcaaa caataatatc
    10921 acaaaagaaa ctaatgtcct tgttctttgt gcaggtggag gtacaagtgg cttgcttgcc
    10981 aatgccttga acaaagcagc agcagaatac aatgttccag tcaaagcggc agcgggtagt
    11041 tacggtgctc accgtgaaat gttgccagag tttgacttgg ttatcttggc tccacaggtc
    11101 gcttcaaact tcgatgatat gaaggctgaa acggataaat tgggcattaa acttgcaaaa
    11161 actgaaggag ctcaatatat caaattgaca cgtgatggac aaggtgctct tgcatttgtt
    11221 caacaacagt ttgattaata aatttgtaaa agttatttga gacgagatta ttctttcaat
    11281 tccttataat attgttaacc atatcattgc atttcatttc cttggtttcc ggaactcaga
    11341 atatcgtctc aaatgctttt tttgaaagga tttatagtta tgactaaaac acttcctaaa
    11401 gattttattt ttggtggtgc tacagctgct tatcaagcag aaggtgcgac tcacacggat
    11461 ggtaaaggcc cagttgcatg ggataagtat cttgaagaca attattggta cacagcagag
    11521 cctgcaagtg atttttacca taaatatcct gttgatttag aattagctga aaaatatggc
    11581 gttaatggta ttcgtatttc aattgcctgg tctcgtattt tcccaactgg ctatggcgaa
    11641 gtaaatgaaa aaggtgttga attctaccat aatctttttg ccgagtgcca caaacgtcat
    11701 gttgagccat tcgtgacttt gcaccacttt gacacaccag aagttcttca ctcagatgga
    11761 gatttcttaa accgcgaaaa tatagaacac tttgtagatt atgccgcttt ctgttttgaa
    11821 gagttcccag aagtaaacta ctggacaact ttcaatgaaa ttggcccaat tggagatggt
    11881 caatacttgg ttggtaaatt ccctccaggc attcaatatg atcttgccaa agtcttccaa
    11941 tcacaccaca atatgatggt ttctcatgca cgtgctgtga aattgtataa agataaaggt
    12001 tataaaggcg aaattggtgt agtacatgcg ttgccaacca aataccctta tgatccagaa
    12061 aatccagcag atgttcgtgc cgctgagtta gaagatatca tccataacaa atttatcttg
    12121 gatgcaacct accttggaca ctattcagat aaaacgatgg aaggggtcaa ccacatccta
    12181 gctgaaaatg gtggagaact tgatcttcgt gatgaagact tccaagcact tgaagcagct
    12241 aaagatttga acgatttcct tggtatcaac tactacatga gtgattggat gcaagccttt
    12301 gatggtgaga ctgaaatcat tcacaatggt aagggtgaaa aaggaagttc taagtaccag
    12361 attaaaggtg ttggtcgccg aatcgcacca gattatgtgc ctagaacgga ttgggactgg
    12421 attatttatc ctgaaggctt gtatgaccaa atcatgcgag tgaaaaatga ttatccaaac
    12481 tacaagaaga tttacatcac tgaaaacggt cttggataca aagatgagtt tgtagataat
    12541 accgtttatg atgatggtcg tattgattac gtgaagaaac acttggaagt tttatcagac
    12601 gcaattgcgg atggtgcaaa tgttaaaggt tacttcatct ggtcacttat ggatgtcttc
    12661 tcatggtcaa acggatacga aaaacgttat ggactattct atgtagactt tgacacgcaa
    12721 gaacgctatc ctaagaaatc agctcattgg tataagaaag tagcagaaac tcaagtgata
    12781 gagtaatatt acttgaaaat gtaaaaaatt gttatccgat actttagaaa aagtacaaca
    12841 cttatttaga ccaggctttg aaaaggtata tattgttagt ttcgaagatt gtcctatgat
    12901 tcctgagctt gaagctaccc cgttgctaaa atgtggtaaa tggtatgttt caactggaaa
    12961 agagtggatt tgccattctg atttagagtt atcagcattt gaatgggaat ttttacaatc
    13021 actcgatgta gaaatacgtg agactattca cttcgaggta aattatttgc ctttccaata
    13081 ataaaaagaa aggaattagt tgtgactatc gaattaaaaa acgagtatct tactgttcag
    13141 tttaaaacac ttggtggtca actgacttcg attaaagaca aggatgggat agagtatctc
    13201 tggcaagctg atccaaatta ttggaatgga caggcgccta ttctatttcc tatctgtggt
    13261 agtttacgaa atgattgggc tatttataga cctcaagaaa gacccttttt tacaggtctt
    13321 attcgtagac atggttttgt tcgaaaagaa gaatttattc tggaagaagt caatgataat
    13381 agcgtgacct ttagtattaa accaaatgct gagatgcttg ataattacct atatcagttt
    13441 gaactgagag ttgtttatac cttgaatggt aaatcaatta gaaccgaatt tcaggtaacg
    13501 aacttggaaa tggaaaaaac aatgccttac tttattggag cccatccagc attcaatagt
    13561 cctttagtag aaggtgaaaa ctatgaggat tattatattg aatttagtga ggttgagtct
    13621 tgctcgatac caaagagttt tcctgagaca ggattattag atttgcaaga tcggacacct
    13681 tttttggaaa atcaaaaaat tctcgatttg gactactcac ttttttccca tgatgccatt
    13741 acacttgacc agctcaagtc tcgtagcgta acgcttcggt caaggaagtc tggtaaaggt
    13801 cttcgagtag attttgatga ttttccgaac ttgattctgt ggtctacaac taataaaagt
    13861 ccattcattg ctttggaacc ttggagtggt ctctccactt cacttgaaga aggtaatttc
    13921 ttagaagata aaagacaggt aacgaaaatt tcacctcaag aaacttctag aaaaatctat
    13981 gatattacta tccttaatta aaatttgtta ttatttgagt cttcaataag atttgaatac
    14041 tcttaccttg tgatgataga ttggtcttct ccgtgccagt cttacatcgc aaggttttta
    14101 agttttttgt gggtctatgt caactgtagt gggtgacgaa aagctaatat ctaaagacag
    14161 tctggtagta aaaaataaga ttatttacta cttcttgatt taaaataata gtggagccat
    14221 taatttgtgg caaatttaat ttaatatttt gcctcaaatt agccacactt tttatagaac
    14281 taagaggaaa aatgaaattt tcttcctatt aatatagttg tttgaaatct taaatatagc
    14341 gt
//
LOCUS       DARBDV010000074        13999 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000074 DARBDV010000000
VERSION     DARBDV010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.075"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            261..2732
                     /locus_tag="SCM56_002135"
     CDS             261..2732
                     /locus_tag="SCM56_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEG1110885.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2835..4571)
                     /locus_tag="SCM56_002136"
     CDS             complement(2835..4571)
                     /locus_tag="SCM56_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEG1110886.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4568..5272)
                     /locus_tag="SCM56_002137"
     CDS             complement(4568..5272)
                     /locus_tag="SCM56_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1110887.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5403..5906
                     /locus_tag="SCM56_002138"
     CDS             5403..5906
                     /locus_tag="SCM56_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HEG1110888.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5866..6204
                     /locus_tag="SCM56_002139"
     CDS             5866..6204
                     /locus_tag="SCM56_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEG1110889.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6225..7643
                     /gene="ffh"
                     /locus_tag="SCM56_002140"
     CDS             6225..7643
                     /gene="ffh"
                     /locus_tag="SCM56_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEG1110890.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7755..8333
                     /locus_tag="SCM56_002141"
     CDS             7755..8333
                     /locus_tag="SCM56_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEG1110891.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8337..8858
                     /locus_tag="SCM56_002142"
     CDS             8337..8858
                     /locus_tag="SCM56_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEG1110892.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8937..9491)
                     /locus_tag="SCM56_002143"
     CDS             complement(8937..9491)
                     /locus_tag="SCM56_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEG1110893.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9744..10019
                     /gene="rpsP"
                     /locus_tag="SCM56_002144"
     CDS             9744..10019
                     /gene="rpsP"
                     /locus_tag="SCM56_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEG1110894.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10031..10282
                     /locus_tag="SCM56_002145"
     CDS             10031..10282
                     /locus_tag="SCM56_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEG1110895.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10407..11198
                     /locus_tag="SCM56_002146"
     CDS             10407..11198
                     /locus_tag="SCM56_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110896.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11368..11889
                     /gene="rimM"
                     /locus_tag="SCM56_002147"
     CDS             11368..11889
                     /gene="rimM"
                     /locus_tag="SCM56_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEG1110897.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11879..12640
                     /gene="trmD"
                     /locus_tag="SCM56_002148"
     CDS             11879..12640
                     /gene="trmD"
                     /locus_tag="SCM56_002148"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEG1110898.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12776..13123
                     /gene="rplS"
                     /locus_tag="SCM56_002149"
     CDS             12776..13123
                     /gene="rplS"
                     /locus_tag="SCM56_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEG1110899.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(13255..>13999)
                     /locus_tag="SCM56_002150"
     CDS             complement(13255..>13999)
                     /locus_tag="SCM56_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1110900.1"
                     /translation="LSNKYVSVIKSKLNLLFSYAMKKGYVEKNPINEVVIDYKRESKT
                     IKIKDKFLEDDEYKRLVNFTTSHNKRYSLLFQWLYLTGMRPGEAIALSKDDVHITNNN
                     ASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAIYNELLELNPNGQFLF
                     QTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKLAELGTPLYAIQDRVG
                     HENSDITEKIYLHVTKGVKEKLKEDIEKL"
BASE COUNT         4590 a         2543 c         2855 g         4011 t
ORIGIN      
        1 ttggtaggat gacgttttgg tcgactaaat tttaccaaaa gtgagtctct ttttgagttg
       61 aaacgaagaa aaagtgttag tagaaaatcg ccttcgattt tccaccaaca caaaatatta
      121 tagaaccctt tttttttgct atatgacagg aatgaaaggt gtttgcaagc ttaatgtgag
      181 gaacactttc catatctgtt ctatactgga taggagaagt ttggaaatat ccagaaaacg
      241 aaatcaatcg aggtagccac atgaagaata aatggataaa tcgaagtaaa aaaaagaaag
      301 taacacaaag atcatcagat aaagtcatcc gtctttttcc atcgtataaa gaaggattaa
      361 cggcagatca agtcaaggaa cgaatagaaa aaggtgcagc aaataattca gtcgatccca
      421 cattcaaaac aaatcagcaa atcgtattgg aaaacatatt tacgtatttc aatttgatct
      481 ttcttatctt agccattttg ctttgtttag tagaatccta taagaacttg acttttttac
      541 cagttatcat agccaataca ggaatcgcca tttttcagga aattcgatcc aaaaagatct
      601 tggacgaact aaatgtttta aatacagcaa aagtcaatgt catacgagac gggatagaac
      661 aaacaataga tatagaagac cttgtaatag atgacattgt tattttagag actggatatc
      721 aaattccagc agatgcagag gtcgtggaag ggaaactgca agttaatgag gctttattga
      781 caggcgaagc agatgaaata accaaagaaa taggtgatcc attaatgtcc ggcagtttca
      841 ttgtttccgg aaaagcttac gcccgattag acaaagttgg agcagattcg tatatttccc
      901 agctgacagt caaagccaag acaatgggag aaggagaaca gtcggagatg attgcttctc
      961 ttaatcaatt gatcaaatgg attgggatca tcattattcc tatcggtatc ctgctttttt
     1021 ctcaaagtta cttttttaat ggaaacacga tcaaagaaag tgtcgttgca atggaagcag
     1081 cattgatcgg tatgatacct gaaggactgt acttgctgac gacgattgca ttagcattaa
     1141 gtgcgactaa actagcaaag caaagagttc ttttacacaa tatgaaaagc attgagacat
     1201 tggcaagggt aaatgtcctt tgtgtagata aaacaggaac gatcacagaa aataaaatgt
     1261 ctgttcaaaa aatgatcgtc tctaaaaaac aagaaataat cagtcagtca gatcaattgg
     1321 aagcatgcat tgctgactat gcaaaagcga tggcaggaga caatgcaaca atggaagctg
     1381 ttaaagcata ttttacaaaa accactgatc aatcctatca ccatgttatt cctttttctt
     1441 ctgtccaaaa attcagcagt gtagcgttgg cagacaaggt gtatgtttta ggagcgcccg
     1501 aaatggtttt gcgcgatcaa ttgccggaat atgcggaaga gtttgtttct ttcgctgaaa
     1561 aaggctatcg cgtgttggtc tttggtgtgt acgatgggat tcttgaagaa ccagtattga
     1621 cagaagcagt cacaccatta ggctatattc tgattgctaa tcctatcaga aaagaagcaa
     1681 gagccacatt tgattacttc aaaaaacagc aagtagccat caaagtgatt tctggagata
     1741 atccgctcac cgtatccaat gtagctgtcc aagcagggat ttcacatgct gatcagtacg
     1801 ttgatgcatc taaattatca gaagaagaat atgaagcagc tgtcgaaaaa tatacagttt
     1861 ttggacgcgt caagccagaa caaaagaaaa tcttcgttca attgataaag cgtaacaata
     1921 cagtagctat gacaggtgat ggcgtgaatg atattctagc catgaaagaa gcagactgca
     1981 gtatagcgat ggcttccgga aatgaagccg ccatgcaggc ctcacaagtg gtcttacttg
     2041 attctgactt ttcacgcatg ccagaagttg ttgcagaagg acggcgcgtt gtgaacaata
     2101 ttgagcgatc agccagcttg tttttagtga agaatatttt ctcctttttg ctctcactgt
     2161 tttcagtgat ctttgctctg acctatcctt tagaaccatc acaaatcaca ttgattagtt
     2221 tgtttactat cggacttcca tctttcttgc ttgctctaga agaaaacaaa aagcgaatca
     2281 gagggaaatt catcatgaat gtgatggaaa aagctgtgcc aggtggacta acagatatga
     2341 tcgtagtcgg tgcgttggtg atctgcgggg tgactttgaa tctaaataaa acagatgttt
     2401 ctacagcatc taccatgctt ttgattgcag tagggttcct cgttctctat aaaatctgtt
     2461 caccgctgaa taaattcaga agtcagatca ttcttttctg tgctagcggt aacttttttt
     2521 cggtcatctt tttgcataag ctgttttcaa tcaccggtat ctcagctgta tcacttttgc
     2581 tgttgagcat tctatttttc tcggcagatt cgatttttag gcatttgacc acattggtag
     2641 aatatttgtt tgaacgtaaa aatgaaaaag agcaaaagcc gtttagtcgc gtagctaaaa
     2701 aatttctatc gatatttaaa cgagaaaatt gacgaaaagt atctcaaaaa agagtaaaaa
     2761 agaacaacca ctcgtgtatc tttcgaagca ggatgagtca tccaacttta aaacgcacga
     2821 gcagttgctc ttttttacat tttcggaatg gtaacggtga aagtcgaacc tgttccaaga
     2881 tgactgtcta cagtaatcgt accattaagg agttgcacgt agtcttttac aatcgctaac
     2941 cctaatccgg tcccgcctga atgtcggctt cgcgccttgt ctacgcggta aaaacgctca
     3001 aagatccgtt cctgatcctc aatgtcgata cctataccaa agtcttggac agaaaacgat
     3061 agatcatctg ttgcctgata gcggataata atctcgctgt cgctttttga atattggaca
     3121 gcattttcaa ttagattttt gataattgga taaaggatat ctgttttagt agtaaatggt
     3181 tcgttctttc caatcaatcg aatcgtcaac cgtttctttt cgatgattgg ctgataattt
     3241 tgaacgattt gctggaaata aggttctgta taaattgtct ggatctcgta agaattctcg
     3301 ccatcttttg acagctggat gatttcccga atcagtttat ctaaccggat cgcatctttt
     3361 tgcatgatct caagaaaaga agtcagagtt tgcgggtcat cttttgcacc atccagtaaa
     3421 gtctcggtaa aaccaatcag tgaagtcaca ggagttttta attcgtgaga gacgttgcca
     3481 acgaaatcct tttgtagctt ttctaaacgt cgcactttcg ttaagtcata cgccacccct
     3541 agaatctgtc cggtaccttc tgaattagta aagtagcgca aactcaagtc taatgtctgc
     3601 cgaccttctg taagggtgat ctcctgatgg atcagcggat gctcaggagt cacttgatga
     3661 atcagctgaa tgagttttgg ctcttgaatg acttctgtgt accgctgttc aggttgatag
     3721 cttaccacgt ttaaatgtag ctgcatcttt ggattaagca gtagtaaacg agaatcagca
     3781 tcatcaatca ggaatacgcc aatcatcagt tcgttcagta acgtatataa ttgctcttct
     3841 gtggtagtac acgcccgata catcttactc atttgttcgc tcaacgcatt aatcgtctga
     3901 tacaattctt cccattgatc gggtgtttgc atgattactt ccgcctgatt aggattttgc
     3961 accatttttt ttagtacggg cagcacggtt ttcaatggct ggtttttttg atagatcaga
     4021 taatagatca ttcctgcaag aacaaggaag aaagacagaa agaagaaaaa gacccaacgg
     4081 cgaaaactct cagttctcgg taaaaaaccg cttgtcggtt ctgcgatacg taaaatggct
     4141 tctaactggc cacttttttt gactggtaat gcgacatata aaagatcctc attcaaagta
     4201 gtacttttgc gcaaagcgct acctaggttc ccaccattca atacggcttt gatttcggga
     4261 cgggagtttc gctgttcatt caatgtttca ttgtttgtgt caaataagat ctttccagta
     4321 gcatccatta aagtaatccg ttcgttcgac tgatggacga attcttccaa agcagttttg
     4381 ttttgcgctg cttggaggtt ttcgacatct aattgccgga tcagcagttg tgcttttttt
     4441 gtcaggtagc tttcttgctg ttccacgatc tgctgttgaa aataatggga aatcaattgc
     4501 cagccaccaa aaaacaaact aactaaaaga ataagaaaga agagacgttc ataaattttt
     4561 ttccatttca tcgtttgggt tcctgaaact tatagccaaa tcctctaata gtcaccaagt
     4621 actttgggtg ttttggatct cgctcgattt tttctcgcaa atgactaacg tgtacatcta
     4681 cgattcggct ttgtcctgcg aagtcaaaat tccaaatgcg gtctagcaat gtgtctcggt
     4741 cgatcacacg gtcttttcgt ttcatgaaat aaacaagtaa ttcaaattct ttcggtgtta
     4801 gttcaatcag atggtcgtct acggtcactt gataattggt caaatcagca gtgatttgtc
     4861 caatcgatag atattcaggc tcagcttcat cttttgattg cttacgaggt tcaattcgtc
     4921 tgaatattgc tttcatacgg gctaatactt cgcgaggact aaatggtttg gtcaaatagt
     4981 cgtcagcacc tatttctagg ccaatgatcc gatccacttg gtcatcttta gccgtcagca
     5041 tcaaaatcgg tgtttcattt ttttcttgac gcagccgctg agtgatctcc atgccatcca
     5101 tcgttggaag catcacatct aaaatgatga aatcaaatga ttgttctaat gctaattcta
     5161 atccttcgcc tccatctgtg gcactggtta ccttgtagcc ttctttttcc aaattgaacg
     5221 tcaagagcgt tacaatagaa ggttcatcat caacaactag tatttttttc attcaaatct
     5281 cctttaaata ggtttcattc tgtttatatt ttagcataga atcagtacaa agggctattt
     5341 tacaaaagaa ataaatggta taataggaag gcactttgct gaatagaaag gaagagcagt
     5401 caatgattgg aatcagtgca tgtttaggcg gtctggcctg tcgctatgat ggacagaaaa
     5461 aagaagtacc agaattgatg gaactagttg aaactggaga agctgtcatg gtctgtccag
     5521 aagtgatcgg cggattacca attcctagag atcccgctga aatcattggt ggaaatggat
     5581 tcgatgtgtg gaaagatcaa gcaaaagtat ggacgacaag cggcgaagat gtaacggaag
     5641 cgtacaaaaa tggcgcgatc aaagcctatc aaaaattgca ggaaaaacac attgatctag
     5701 tcatcatgaa ggaaaaaagc ccttcatgcg gaacccattc gatttatgac ggaagttttt
     5761 ccggaataaa aaaaaatggt gccggagtgg ctactgctta ttttattcag caaaaaatga
     5821 ctgtgctctc tgatgaagaa tggctgaaaa ctagaggtga gcagaatgga aatcgaaaag
     5881 accaatcgaa tgaacgcgct ctttgaattt tattcgacct tattgacaga aaaacaaatg
     5941 aactacatgg aattatatta tgcagatgat ttttcattag gtgagatcgc ggaagaatac
     6001 gaagtaagcc gacaagcagt atatgacaat atcaagcgga caagcaagat cttggaggat
     6061 tatgaaaaaa aactccatct tttttctaat tatattgttc gagaacaggt attggaaaat
     6121 atgactgcat atattacaga taagtacccg gaagataaaa aactacttga atatgtacag
     6181 caaattcaag caatcgaaga ataaataaaa ggaatgaaga aaatatggca tttgaaagct
     6241 taactgaacg tctccagcag gcgatgggaa aaataagaaa aaaaggaaaa gtatcagaag
     6301 ctgacgtaaa agaaatgatg cgggaaatcc gccttgcctt actagaagcc gatgttaact
     6361 tgcaagtagt caaagatttt actaagcgcg tacgcgaacg tgcggtaggg gcagaagtgc
     6421 tggaaagttt atcacctgca caacaaatcg taaaaatcgt tgatgaagaa ttgacgatca
     6481 cacttggttc tgaaacagca gagttaaata aatcaccaaa aataccaact gttatcatga
     6541 tggctggtct tcaaggggca ggtaaaacga cctttactgg taaattagcc aattatttaa
     6601 agaaaaatga aaatgctcgt ccgctcctaa tcgcagggga cgtatatcga ccagctgcga
     6661 tcgaccaatt gaaagtatta ggtcaacagc tagacgttcc tgtatttgat atgggaactg
     6721 aagtcagccc ggtagagatc gtccgtcaag ggatggagtt agcaaaagaa aagaaaaacg
     6781 actatgtatt gatcgatacg gcaggacgtc tgcatatcga tgaaacattg atggatgagt
     6841 tgaaacaaat caaagaattg acgcaaccaa atgagatttt gcttgttgtc gatgccatga
     6901 ctggtcaaga tgccgtcaac gtagctgata gcttcaacca gcaattaggt attacagggg
     6961 tcgtcatcac taaacttgat ggggatactc gaggcggggc agcactatcg atccgctcag
     7021 tgacaggcgc accaatcaaa ttcatcggtt ctggtgaaaa attgactgac ttagaagtat
     7081 tccacccaga tcgtatggct agccgtatcc ttggtatggg ggatatgctg acattgattg
     7141 aaaaagcgca gcaagactac gacgagaaaa aagcggaaga acttgcacag aaaatgcggg
     7201 aaaattcatt cgatttcaat gactttattg aacagcttga tcaagtgatg ggtatgggcc
     7261 cactggaaga tctaatcaaa atgatcccag gaatgaatca agtccctgga atcgaaaacg
     7321 tcaaggtaga tccaaaagat gttgaacgca aaaaagcgat ggtttattct atgacgccag
     7381 cagaaagagc aaatcctgat ctgttgaacc caagccgtcg tcgccgaatt gctgccggtt
     7441 ctggaaacag tgttgttgaa gtgaatcgaa tgatcaaaca attcaaggaa tcaagaaaaa
     7501 tgatgcagca aatgtccaaa ggggatatga acatcccagg tatggaccaa atgtttggtt
     7561 caggcgtcaa aggaaaacta ggcaaaatgg cgatgaatcg aatgatcaag aaaaataaga
     7621 agaaaaagaa aaaacggaaa taagatgaaa aaaacagctt cggtacagac tgaaaatgtc
     7681 tactgccgga gcttttctat cagcaatttt tattctttac taatacaatt actctaaacg
     7741 aagaggtggc acagatgaga tgtccgtgga gtgaacaaac agaaagtatg cggcaatatc
     7801 atgatactgt ctggggtgtt cctgaatatg atgaccaaaa gctgttccga aaattgatgc
     7861 ttgatattaa tcaagccgga ttgagctggc agacgatttt aaataaaagt gaagcattcg
     7921 atgaagcata tgatcgattc gatatcaaaa aagtcgcaaa ttatggagaa acaaaaatca
     7981 gtgaactaat ggaaaacaaa ggaattattc gtaaccgaag aaaaatcgaa gcagccgtcc
     8041 aaaacgccaa agcagtcttg aaaattcaag aagaaatcgg cagtttttca gagtatcttt
     8101 ggtcgttttc taatggaaaa atcgttgatt cttcttatca ttcacaagaa gaagtcccaa
     8161 caacaagtcc tctttcagat gaattagcaa aagatctaaa gaaaagagga tttaaattta
     8221 tcggaagcac aacaatctat gcttttttag aagctgtcgg gatcatcaat gatcaccttg
     8281 acagttgttt ccgcaaagaa gaggtcgtac gtatcggcag aaagggcgaa taaagcatgc
     8341 gaaaaatcat attttatggt gctataagtt tagatggcta tttggcaacg aaaacggatg
     8401 atcttcaatg gctttttgac actccaacag gtaagcaaac tacttatgaa gctttttatg
     8461 aaacaatcga tacgaccatc atgggcagaa aaacttatca ggaagcaaaa aaatatttgg
     8521 agaccgaagc aatttatcca gagaaaaaga atttcgtttt ttccacccac gcacaattac
     8581 aattgccaga tgctgaggtc gtaaacgaag atcctgttgc ttttctaaaa aatctacaaa
     8641 tgacagaagg aaaagatatc tgggttgtgg gtggcggtcg attgatcaaa ccgctattgg
     8701 aacatcaaat gattgatgaa tggtatatcc agattacccc tgtattatta ggggacggga
     8761 ttcctttatt ccaaaaagga acatatgaag cgcgttttga aatgctagac acgacacgat
     8821 tcggtgaatt tatcgaattg cattatgtac gaaagtagaa gtaaataaaa taaaaaaggc
     8881 aactatatgc agaaatgtcc tgtacatagt tgcctttttt attaccgtta tgctttttac
     8941 tctgttaatt tgttagaaaa atctagatac gcatctaaaa tcgtttggaa gaaacttaat
     9001 gttccttcaa cgatattacc attttcatca attagattcg caacgtttcc gatatatgct
     9061 tccggttgct gcaaggttgg cacattcaaa aagacaagtg attggcgtag atggtggttt
     9121 gcgccaaagc cgccgatccc acctggtgat gcagtaacga ctaaacctgg tttatgatcc
     9181 caaacactgt gtccataagg tcttgagcct acatctaaag catttttcaa tactgctggc
     9241 acagaacggt tgtattcagg agtaacgaaa acgacaccgt ctagtcccct cacttcctca
     9301 cgaaaacgag tccattcagc aggagcctgt tctggagttt cgagatcctc attgtaaaat
     9361 ggcagatcat cgatttttac aaatacactt tcgtaccctt caggaagcaa tttcgcaaaa
     9421 gcttctgcta cttttttgtt gtaagaatct tttcttaaac taccaataaa aaatccaatt
     9481 ttttttgtca tacgattttt cctccttcaa actatctata ataatcttac aaaagataag
     9541 taactggagc aaataaaaac acttactgac aaaagctaca tagaagatag attacttatt
     9601 tttaacaaaa aagaagtcta aaatcttcta ttttctaaaa aaaatcgaat ttaatcaagc
     9661 tgtaaagaaa aaaagcttta cagggtaaaa aaaaactgtt acactacatc ttgtgaataa
     9721 aacaatggag gtggaaataa ataatggcag ttaaaatccg tttaaaacgt atgggttcta
     9781 aaaagagtcc gttttaccgt atcgtcgtag ctgattcacg ttctcctcgt gatggacgtt
     9841 tcatcgaaac tgtaggtact tacaatcctt tgaaagaccc tgcagaagta gttttaaaag
     9901 aagacttagt tcttgactgg ttatcaaaag gtgcacaacc ttctgacact gtacgtaaca
     9961 tcctttcaaa agaaggcgtt atgcaaaaac accacgaagc taaattctca aagaaataag
    10021 gtgaccggat atgaaagatt taagcgactt agtcttaaca atcgttcgtc cgctagtcac
    10081 ttatcctgat caagtcaagt tagacgtcgt agagtcgaaa gacttttacg agtacaatct
    10141 aacagtggct cctgaagata ttggccgtat cattggaaaa caagggcgtg ttgctaaagc
    10201 aatccgcaca atcgtttacg gtgttcggat caatgcaccg aaaaaagtgc gtttgaatat
    10261 catcgatgat aaggaaaagt aaataaacgt gctgtactcg aattttcgag tgcagctttt
    10321 ttgttttttt tattaattga agaaatgatt atttggcaaa acaagcaaga aagcgtatcc
    10381 ttataggagg agggagtgaa cagccaatga gaaagaaaat gttatttatt ccatttgctc
    10441 tattgttaag cgcttgtagt tcggttactc aagaatcaga caatacacat ctatcaagca
    10501 gtatatctga aactgttacc agtccagaaa aaagtagtac caaaacggct tccaccactg
    10561 aacaaacaat ggctacctca aataatgaga aaaagactgc tttagaccag ttgaaagaac
    10621 aacagccaaa tgttcccatg cctctagatg taccagtctc ttcaggctat ctcaatatcg
    10681 cagcgacaca tacaaagcaa ggttattcta ttttatatta cagaacagat cgtcccctcg
    10741 ggttgaatgc tgacgaattg aatcaagaaa cgcctattgc aacctatcta tatcaatatg
    10801 ggtttgcttc tagtcaagaa acaatccaag ttttgcagcc atttgagatc gacacaaatg
    10861 gtcagcaagt ggacttaggt tctcgaatca cagggtatca gcaaggtgca gcaggttcaa
    10921 gttttcttga atggcaagaa ggaaactggc gtattcgaat ccgtgggaat aatatagaag
    10981 gccaagatcc cttactatta gctaaagaaa tcgtagctta tttggaagaa aacagtctac
    11041 cagcacctga acaatttggt aaaattactg ttgatatggg tgatacgacg aatcgagccg
    11101 tagaagtaag ctggcaagaa ccgaaaaatg cctatacgat cactcatcaa gatcctatgt
    11161 ctgcgttgaa gatggcagtt tctatgaaga ggttgtgaaa tcagcgcgct gatctgagcc
    11221 catcatttaa ctccaagtca caggaagtaa tcaaaaatag cacgccgcat ttttgcataa
    11281 ttttggctta ataccttaaa agaaaactag aattgcctct tcacacatgc taaaataaaa
    11341 cagacaaaaa agaaatgggg atataagttg actgaatact tgaatgtagg aaaaatcgta
    11401 aacacacaag gtatcaaagg cgaagtccgt gttatctcaa caacggattt cccagaagaa
    11461 cgttacaaaa aaggaacggt gctaacacta ttccaagacg gaaaagcacc tgtggaatta
    11521 acagtcaaaa gtcatcgcaa acataaaaat tttgacttat tgagttttga aggacatcct
    11581 tctatcaatg atgtggaaaa atatcgggat ggcatattaa aagtgtcaaa agacaactct
    11641 gtagatctag ctgaaaatga attctattac catgagatca tcggtctgca tgtattagaa
    11701 gatggaactg agttaggtaa agtaaaagaa atcttgtcac caggtgccaa tgatgtttgg
    11761 gtcgttcaac gtccgaaaaa agctgatata ttgcttccat atatcgcttc tgtcgtcaaa
    11821 gaagtcgatt tagaagctgg agtggtccgt gtagaaattc cagaaggact gattgacgat
    11881 gaagattgat gtattgactt tatttccaag aatgtttgaa ggtcctatgg gggaatccat
    11941 catcggtaag gctgtagata aaggattatt agatatcaat atctctaact tccgcgatta
    12001 ttcagataac aaacatcaga cagtagatga ttatccatac ggcggtggag caggtatgct
    12061 gctcaaagtc cagcccatct atgataacat ccaagccatt gaaaaagctg caccggatgt
    12121 caaaaaaaga gtaattcttc ttgacccagc tggtaaacga ttcgatcaga aaatggcaga
    12181 agaattttca caagaagaac acttagtatt catttgtggt cattatgagg gatatgatga
    12241 acgtatccgc acattagtga cagatgaagt ttccttagga gattatgtcc ttactggggg
    12301 agaattaggt gcgatggtca tgatcgatgc aacagttcgg cttttgccag atgtgttagg
    12361 gaatcagact tcagctcaaa cggattctta ttcaaccggt ttattggaac atccccaata
    12421 tactcgcccg gcagaatata aagggatgaa agtaccagaa gtattgacaa atggaaatca
    12481 taaattgatt gaagaatggc aattaaaaga atcactaaga aggacctatc aacgtcggcc
    12541 tgatcttttg gaaacacttg aatggacacc tcaaatgacg aaatttttag aagaaatcaa
    12601 gaaagaagaa caagaaaaaa cagcagatga agccaagtga ctttttctaa aatcttcttt
    12661 acagtaggtt gataatatgt tagtatttta tggtgagtgc aaaagcactt aactattacg
    12721 atattccgct gtgagaaaag ctcataagaa tatttggaat aaggagaaga aaaccatgaa
    12781 tccattaatc gaagaattaa caaaagaaca attacgttct gacatcccag cgttccgccc
    12841 tggtgacact gtgcgtgttc acgcaaaagt tgttgaaggt actcgtgaac gtatccagtt
    12901 atttgaaggt gttgttatca aacgccgcgg tgctggaatc agcgaaactt atacagtacg
    12961 taaagtttct aacggagtgg gcgttgaacg tacattccca ttgcatacac cacgtgttgc
    13021 aaaaatcgaa gtagttcgct acggtaaagt acgtcgtgca aaattgtact acctacgtgc
    13081 attacacgga aaagctgctc gtatcaaaga aatccgtcgt taatcatcgt tatcagactg
    13141 agaaagaacc cttggtattc aagggttctt tttttgttct ctgttttttt atattaacga
    13201 tttctatttg ggacattttt gattaataaa aaggggcaaa aaaggggcaa caagttacag
    13261 tttttctata tcttctttca atttttcttt tactcccttt gtcacatgca aataaatctt
    13321 ttcagtgata tcactatttt cgtgcccaac gcgatcctga atagcataca gaggagttcc
    13381 tagttccgct aattttgaaa tatgggtatg tctaaaaata tgtgaactaa gttttttatc
    13441 aatcttcatg tcagctttat gatttctcaa ataggtgtta attgctgtta gttggaaagg
    13501 agttcctttc gttgtttgaa atagaaattg accattcgga tttaattcta gaagctcatt
    13561 gtaaatagct atcgcttttt ttggtaaatc aatttctcgc attccagcag cagtttttgt
    13621 agaatcagat tttttcatat cagctattga acgttctcta tacatcatcg tcccatttat
    13681 aactactgat gcattattat tggtaatgtg tacatcgtct ttacttaatg cgattgcttc
    13741 tccaggcctc attccagtca aatatagcca ctggaaaagg agagaatatc ttttattgtg
    13801 tgaagttgtg aaatttacta gtcttttata ttcatcatct tctaaaaact tatctttaat
    13861 ttttatcgtt tttgactctc gtttataatc aataactact tcgttgatag gatttttttc
    13921 tacatagcct tttttcatag cgtatgaaaa aagaagattc aatttggatt taattacact
    13981 gacgtactta tttgacaag
//
LOCUS       DARBDV010000075        13863 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000075 DARBDV010000000
VERSION     DARBDV010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.076"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            139..477
                     /locus_tag="SCM56_002151"
     CDS             139..477
                     /locus_tag="SCM56_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110901.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(682..1257)
                     /locus_tag="SCM56_002152"
     CDS             complement(682..1257)
                     /locus_tag="SCM56_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEG1110902.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(1989..2570)
                     /locus_tag="SCM56_002153"
     CDS             complement(1989..2570)
                     /locus_tag="SCM56_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1110903.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2753..3379)
                     /locus_tag="SCM56_002154"
     CDS             complement(2753..3379)
                     /locus_tag="SCM56_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEG1110904.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3401..4732)
                     /locus_tag="SCM56_002155"
     CDS             complement(3401..4732)
                     /locus_tag="SCM56_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEG1110905.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4887..5288)
                     /locus_tag="SCM56_002156"
     CDS             complement(4887..5288)
                     /locus_tag="SCM56_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEG1110906.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5374..5580)
                     /locus_tag="SCM56_002157"
     CDS             complement(5374..5580)
                     /locus_tag="SCM56_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110907.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5675..7177)
                     /locus_tag="SCM56_002158"
     CDS             complement(5675..7177)
                     /locus_tag="SCM56_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEG1110908.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7195..7650)
                     /gene="argR"
                     /locus_tag="SCM56_002159"
     CDS             complement(7195..7650)
                     /gene="argR"
                     /locus_tag="SCM56_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEG1110909.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7748..8365)
                     /locus_tag="SCM56_002160"
     CDS             complement(7748..8365)
                     /locus_tag="SCM56_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEG1110910.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8586..9959
                     /locus_tag="SCM56_002161"
     CDS             8586..9959
                     /locus_tag="SCM56_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1110911.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10034..11146
                     /locus_tag="SCM56_002162"
     CDS             10034..11146
                     /locus_tag="SCM56_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEG1110912.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(11218..12855)
                     /locus_tag="SCM56_002163"
     CDS             complement(11218..12855)
                     /locus_tag="SCM56_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEG1110913.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13023..13631)
                     /locus_tag="SCM56_002164"
     CDS             complement(13023..13631)
                     /locus_tag="SCM56_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG1110914.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3761 a         2902 c         2472 g         4728 t
ORIGIN      
        1 actaaaaaaa taatccaaga aacattgaac ctatggatcc aaaagctacc caaatactat
       61 agctaacccc ccgctacctt caattttgca gataaaacgc tttgggagaa aatttcaaag
      121 acaacaggcc atcctatcat gcccgattat aaacaaaaag ctggctattc cttttgctct
      181 gacaaatttc aattgggaaa gttcacgaaa ctagcaggtg aagaagtttc aactgttcgg
      241 atcaaagaat gcccgcttca aatagaaaca ttggttcaac gaatttcaac tagacaagac
      301 tttgctattg tagaacgtga gatcgtagct atttttgtag aagaagggat tttacatgat
      361 ccaactcata tcaatgtgga taaatggcaa cctcttatct ataaattcag agaatataca
      421 actgccaacc agtcgttaac tttgaatttt agattccaag aattcaaaga gtcataatga
      481 taaacatcgt taggtataaa taaaaaagac tcgcagcacg agtaaaaaac aaatagaagc
      541 tatacattag aaaggtcgac tgtgacccta tagatgcagt atatgcagct tatgagcatt
      601 tcttctgttc tctttgtttt tgggatctgt gctgaagtct ttttcgtttt ttgccgctct
      661 ttctttgaaa aatcgattat gttatacctt ctccattact aattcaggca tctgctgagt
      721 aatgattttt cgcgcaaaag aaatatctgt tacccaaggt tcgatttgtt cttcagtcaa
      781 gacaagcggc attcggtcat ggattggtgc gactgtgcgg ttaggttcag tagtcattat
      841 gattgattct gccatttttt catcctgatg gctacgataa aaaccgccaa tatacactat
      901 tttttcctta tttgaaaaca taaatttgtc tttttcttca ttccactcat aaaagccact
      961 cattgggaaa acacaacgat tttgcaaaaa tggttgatga aaggtctttt tttcttctac
     1021 tgtctctgca cgggcattaa tcatcaattg tcctttttta aatccggtaa atccccactt
     1081 catgatttcc ggagtgagga tgttttcttc ctcttttctt aacgtcacaa caagatttga
     1141 cggaaaaact tctccagctg ctaattctgc tgcctgcttc ggttgtttcg tctgggcttc
     1201 tcgataataa ttctgaagga gctctgactc tagatcaaaa aaataacgac cacacatcct
     1261 aatccctccc aatctttttt tctatcatac actgcaacaa gaagttcgca ccaacaaaac
     1321 gctttcaatt tttgtagaac taaaaaagtt gtgacaagtg acaaactctc cagtgaatgg
     1381 tattctcctg ccaactcctc ggtcacaagt cacaatattc aacaaacatg agaagctgct
     1441 ttttgaaatt gttccttgtg actcggagtg aaacggcagg cttacgacct ctgggtaaac
     1501 ggtgttcaaa agccagtttc tcggtattaa gccaaattta tgcaaaaatg tagaaagcca
     1561 ttttcacaaa atttttctta atactcgaaa ctaagcgact ttttcacaac ctcttcctta
     1621 aacggtgttc aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aatatgagca
     1681 gctattttta tttggctgtt cttattactt gatgcagggc agctttttca caacctctcc
     1741 actaaacggt gttcaatcag ctaaccttcg gtattagctc aaaaaacggc aaaatatgag
     1801 gaactatttt gccgtttgtt tctctaatac tcaggtcaaa gcgctgattt cacaacctct
     1861 aatacacggt gttccagaag tagcttcttc gaaaaataag ccgaaatttc acaaaaattt
     1921 gagaagcaat tttcgtaaat tccttcttat ttcttgaagc tgaccacttc tgtcacaacc
     1981 tctaattatt aaagaacctt ttgcgaagtt aacaatcttt ctttgatcaa ttgaaaattc
     2041 tgcctaagta gtgatagatc ctgtgtgact ttcaacaata agatacttcc ctggatttca
     2101 gcaatcaacg ctttggcttg acggtctgct tcttcatcgg acagtttttc ttcagaaagt
     2161 gccaggatat tttgtttcgt atacttgacc cattgattga ctaattctag tagcaacaga
     2221 cggtattctt catccactgt actcaactca ataatcaaat tacctacagg acagccatga
     2281 taagtgatct gtgtctcatg atcagcgaaa atccagtcca acatttcttc taatgcttct
     2341 tctggctgtt ttccagcatt taagatgtct tcgaacaatt ctgtctgcca tacgtccata
     2401 tgcgcatgga tcaccgctag acaaacttct ttttttgaag aaaagtaata ataaaattga
     2461 ccttttcctg tatcagaagc tgctagaata tctcggattg acgtttcatg atagcctttt
     2521 ttataaataa gatctttggt cgcttccaag atcttttcct ttgttttcaa tgatattctc
     2581 ctttttcatt tctcatagtc ctatattata cagtaaattc atcatttttt cttcttaatc
     2641 gtttaaaaaa gactgtgcca aattatctga cacagccctc actataaaac tgttctcata
     2701 tagctagatg ttcattgatt caagaactgc gaggacaaaa gcaattccta tcttatgata
     2761 gttgctctaa tacttcctct gttgtcatca cttgatctgc aataaagcca aagttcgtca
     2821 acgctgcatc cattgcttct tggccaggag caccaattgc atctgtcgct accacaacat
     2881 tgaatccact ctctaccaat tcacgcatat gggaatcgct acacaagttt ccgttcattc
     2941 caccaagaat tactgtgtcg attccatttt ttctcaattg gagcgccaaa tcattgcttt
     3001 ctggtccaaa gattttatga ggagagctga tgatcgtatt ttcatccaaa taaggtttca
     3061 attcttcgat aaaatcggct cctgaacctt cttccactga ttcatattga ctttttctag
     3121 caaacatctg ctgctcaagc atcatatgtt ctcctgcacc gccaaattgc catttatggt
     3181 catgtgggta aaaataatga ggagaaataa agagtttgta tcctttttct tttgcttgtt
     3241 ttaccatttc aatcatattt tcgatcgtat tgtatttttc taagatatct tttgtcaaac
     3301 caaagccacc gcctgctggt ttcaagaact cgacttgcgg gtcagtgaga actactgctg
     3361 tatttttatt attgaacatg gtggattctc cattcgttat ttattttaat acatcattta
     3421 gagcttcact cattgtcgga tgagaataga tttgatctct caataacgta taaggaagtt
     3481 ttccattcat agctagcgaa atcaagttga tgacttcatg agaatcttcc gcatagatac
     3541 ttgcccctag aatcaagtca gtttcagggt cgactaaaac tttgaataca cctttaggat
     3601 cttctaatac ttgtgcttta gggacaccgc ttgtcatata cttgaataat ttgtaattga
     3661 cgccttgtgc tttggcttgt ttttctgtta aacctacatt ggataaagtc ggagtgataa
     3721 atacactgta aggcacagct ttccggttat tcgttgttcg tttattctcg cccttcaact
     3781 gatccataat gattcgataa tcatctagag agatataagt gaactgcaat ccgcctttga
     3841 cgtctccaat cgcccaaatg ttgtctgcat ctgtacgaag gtattcatcc acagcgattg
     3901 ctccgcgatc tgttagttta acatctgtat tttctaatcc aagtgatgct gtacttggtt
     3961 ttcttccagt tgcagccaaa acttttgacg cttggattgt tacttcttgg ccatcttctg
     4021 taaaggtgac ggcagctgtt gattcttggt ctgctacttg atctacagat acacctagat
     4081 ggaattcgat tcccgcattt gtcatgtctt ctaaaatcat ttgcgcgata tcttcatctt
     4141 cacgtggaag aaattcagaa tgggcatcaa gaacgacaac tttagatccg tattcattga
     4201 acattgatgc aaattccaag ccaatatatc cagccccgat gatcactaat ttttccggta
     4261 attgttcctg atccatcgct tgcgtagaat caatcaagta gcgacttgta ttcaatcctt
     4321 tgattggtaa aatgattggt tcagaaccag tattgatgaa aatccgttcg ccttcaacaa
     4381 tcactttttc accattattc gtcacttcga tttgatggtt agaaagaaaa cgtgctgtac
     4441 cgtccatcac tgtcccagtt gcctcatcgc tgatcatatg ataatttttg gcgcgaagca
     4501 tgcccgtcaa tttttctttc cgagcaacag cctctgtaaa gtcgattcct ctttctccat
     4561 tgaaaatcaa tgatttagaa ggaatacagc cgatgttgat acatgttccg ccatacatac
     4621 gtgttgactt ttcaacgact aaaaccgatt caccttttga agctaacgcc ttcgctaaag
     4681 tcttaccgcc ctttccaaaa ccgatcacaa tattttgata tttttccatc atttttccct
     4741 cgcttctttt ttacctaccg attggtacaa aaatatgata accgataatt gtctattctc
     4801 caaatcatct gctttatttg tcaaaaaaaa ccagacaaaa agcggtcctt tcacgttttt
     4861 agtctggttc gatttccttt attcttctaa acaatcgtta cttctacatc gatatttcct
     4921 ctagttgctt tggagtaagg gcagaattca tgcgcatctt ctgcaatttt ttgggcttct
     4981 tcttcgctga tgccatcgat atgcacttct aatactacac cgatctgata gccgccatca
     5041 tctaaattat aaagagagac acgtgctttt actgtggact ctgctgtcat tcctgcttct
     5101 ttgatcatat gttgtaccgc cccattgaaa caagaagcat aacctgctgc aaataattgt
     5161 tctggatttg ttccttctgc atggattcct ggaggagtga ttttcatatg catcgagcca
     5221 tttggtgcct cgacttctcc attgcgcccg ccatggttga tcattgctgt ttcgtatgct
     5281 tttttcattg tactgctcct ttcgattgac catttgctac ttggctataa ttatagtgta
     5341 tgacaagtaa ggcgctttct acaaaaattc tgcttattcg aataccagaa tgccgtcttc
     5401 tactattttt ccaaaaaaca aaaaaatact actcgtgaac aacagcgtca tatttgttcc
     5461 aaaataaata gcaatctttt ctacataatc ttttatatga ttgattttaa gatacatata
     5521 taccagaaag ttgaccccat aaaccaatac tgttaaaaaa ataaccatat tcgttaccat
     5581 tttttcctcc ttttgctggt ctccctacca gcactcgatt taaccatacc actttttttg
     5641 ccgtccaagc aactagcctt tcaaaaagac tggtttattc tgctgttaat gattcaagat
     5701 cagaaaaatg tccgaataat tttgctaatt ttactaacgc caacttgttc tttccgaatt
     5761 catcattttc tatctgtaat actagtaacg gctcatgtaa gcttaaacgg atcaaaaacc
     5821 agccgtttcc gtatggctct tctagatcat atcgaatacc ttcttcattt tccgggtcga
     5881 agttcatccc tttttcttct tccaacagtc gcttcatttc ttctaatatc ttgtttcctt
     5941 gttcgcgtgg ttcatcgctg aacaatttta atcggatttc ttgtacttct acgggctgtt
     6001 tcaactcttt gatttgttca tctaatgtga taccttttct ttttaattct ggcaaaagca
     6061 tcaggatctt tgcgatgaca tatgcaccat catccagaaa atagttttcc ttgaatgcag
     6121 cgtgtccgct tgtttcgatg gctagctgtg tatctttccc aagacggtta agttctaatg
     6181 ctttattgat tacatttcga tagccggaaa tataacgaac ttggatgcct cctttttctt
     6241 caatgaatct ttgtagatga gaagaagtag gggaattcgt cacgattctt gttcctggat
     6301 gctcttttaa tacgattgta cttaatactg cgatcaaatt atttcgattg atcactgttc
     6361 cagaagcact gacaactgca gaacgatcca catctgtatc gaaaatgatc ccaagatctg
     6421 cttcatttgc taaaacagcc tgcttgatac tttccatcgc ttctttatta tcaggatttg
     6481 ggatatgatt aggaaatcgg ccatctggtt ccaaaaattg ggaacctgtt gaatccgcac
     6541 ctaagctatt caatactttt tcagcaaaga atccaccagc accatttccg gcatcaacaa
     6601 tgattttcca accatttagt ggtttgcttt ctagtgttcc aatccccttt ttaatttttg
     6661 tgactaaatc ttgcgcataa ggtgtcaaaa ggtctgcttt ttctacgctt ccagtttgtt
     6721 cgctttttat tttttgctgc tgtgatagga taaaagcaat atcttctttt tctgctcctc
     6781 cttctgaggt aaagaatttt attccattat aataataggg aagatggcta gcagtcagca
     6841 taaccgcagc atcgcacgag aactgttcaa attgagtgga cataaacata gcaggcgtag
     6901 tagctaattc gaaatcgatg acatgtatcc cttgttcttt cagactttgg atcaacgcct
     6961 ctttcagtgc tggtccgctt atcctactgt catgaccaat cccaatcgtc cattttttat
     7021 ttggatcggt tgatttcttc tttttcttta tccagtttgc tagtccccag ccgatctttt
     7081 ttaccatgtc tgtcgtcaag tttacttcat atccttcaac tggtaatgcg attcctcgaa
     7141 tatccgatcc attttgcagc gctttcatat ctttcatata aaagtcctcc tcatttattc
     7201 cctcattttt tccagctttt ctgcaaatcg ctgtgcttct tcttctgagc ggcaaataat
     7261 aatgaatgta tctacaccgg ccatcgttcc gacaacttca gggtaagcca cttcatctag
     7321 ccaattcgtt accacatcgg caccatttcg ttcagtcaat acgataacca tgaactgaac
     7381 agactggata cgcaaaacct cgcgtctaat tgctgtacgc aaacgttctt catttaaaga
     7441 aacagatggc tggttaaata atgtatagcg tactattttg ttttcatttt gactttttac
     7501 taacttcatt tctcgaatgt ctcgagaaat cgttgcttga gtcacatcgg cgcctgcttc
     7561 atgcagccgc gcaagcagct cttcctgtgt cccaataggt tgttctgtaa tgatctggcg
     7621 gatcagtgct tgtcgctgtt tctttttcat attagacacc tctcaatgtt gattatagca
     7681 aaagataagt aatatttctt catattttgg tattttttac ttttattttt tgttttcatt
     7741 caggttgcta acctttttct attttaacac gcaaaatcgt cttcattttt tcaggtgctg
     7801 attttcttgg atcgctgatg taaatctctt tgtgttcttt agaagctctt tcatagcctt
     7861 cttttgcgat gaatgcttct aatttctcaa acgttgctgg ttcttcggca tagggcccga
     7921 cgtgtaagat ttgggcacaa agaccttctt ccatcttttt gaatactagt tgttctttca
     7981 agtcttccgg aagtttgcta gaacgctgaa tggcttcttg tgctatcgct tctgtaacaa
     8041 agtcaggctg tcggatcatc aattgataag aaaaatgttc ttttttcatc actggttcat
     8101 gcaaatactt ttcttgcagt ccccattgtc cttccagtgg atagacagta tacatctgat
     8161 aatcaggaat cagccaattt tttttaggcg acatgcgtat tgcatagctg actgcatata
     8221 gacaggctac tcttcgttta aagtcttcct gattcgggtc gccgatccct ctgatgctaa
     8281 agaatggttt tttaggaacc tctagtattt gcgggctttt tttcatacta tagctcttgt
     8341 ctttacgcca ttcaattttt tccatttttc ttcctccgcg aaattccatt tagttattta
     8401 ttatcatacc tctttttgag tgacttggct aatcgtgttg acactatttt tcagtttgtg
     8461 ctagaataag tgaagtaaat aaatcgtaca gataacacgt ttcggcgtgt cgagagagag
     8521 gcggtttcta tttttataat agaaatcact ctcttttttt acgaaatttt ttagaggtga
     8581 tgtaaatgac aaaacaaaaa cagctgcttt tagtacctgc agtaattgca acagggatga
     8641 tgtcttttgc gggcgtattg atcgagacgg ccatgaatgt aactttccca actcttatca
     8701 gacagtttga tttaacgact gcacaagtcc aatgggtaac aacgatttat cttttgatga
     8761 tttcgatcat tgttccgctg tctacttatt tgaatcgcaa ttttagtctc aaaaaactct
     8821 ttttagcttc aaatctgctt ttcttggcag gtataatgat cgatttcttc tctcctacct
     8881 tcatggtcct attgtttggt cgattgctcc aaggtgcagc gacaggtatc gctcttccgt
     8941 tgatgttcca tattatcttg acatttgccc ccctcaatcg acgaggcgcg atgatgggtg
     9001 tcggtacttt gacaacggcc attgctccag caattggccc cacttatgga ggcattatga
     9061 cttcacattt tacatggaat catatcttcc tgtttcttgt gcccgttttg attctttctt
     9121 tgattatcgg ctggtttgct attcccgaga tggcagtaca aaaaagtggt tatttggatt
     9181 ggataagcgt tttaggaatt tgtcttttgt ttagtggaag tctaacattt ttgagtacgt
     9241 tgggaacagt ttccagctgg attgctttaa tcatcggcgg tattggctgg ttcctttttt
     9301 actatcgaag taaaaaagtg gagcatccac tgatccgact gtctattctt tcaaataaaa
     9361 cattccgctt gtttttgatt ggcttcttag tttatcagtt tttattactt ggtgtatctt
     9421 ttgtcttgcc gaatttcctt cagatcgttc aaggaagtag tccttttgtc gctggattag
     9481 cgatgcttcc cggcgcagcg gttggtgctt tgctttctcc tttttccggg aaaatgctgg
     9541 atcaatatgg tccgaagaag ccaatttttg ctgggctgat tctttctttg attggttggc
     9601 tagcattatt cctactcata ggacacgctt cagtgggtgt attgattgct tgtcatgtgt
     9661 tttatatgat cggtatcgga ttatcttaca gcaatatgat gaccaccgga atgaatgcac
     9721 tgaatgaaga actacaagga gacgggaacg caatgttcaa taccttgcag cagttttctg
     9781 gcgctgtggc tacttcatta gtagcggtca ttatcaatta cgtccagcat catacttccc
     9841 ataactatga agtgtctact acattaggat caaaagcagc attaggcgtc ttgttgctgt
     9901 tgcttctcgt aagctttgct cgattcgcct attatctgtt ctttgctaaa aaagcgtaga
     9961 ttccatatac ggcaggtgtt gcatctgccg tttttttgtg tgaaaatggt tgtttgtaaa
    10021 aggaggcttt tttatggaaa aaattgccat tatcggtggc gggatcattg gtatgactct
    10081 agccagttac ttagatacat caaaattcaa tgttgcttta tttgatgaag ggacaggaca
    10141 ggcaactaaa gctagcgctg ggattatctc cccttggttg tcgaaacgca gaaacaaaca
    10201 atggtatcgt ctggctaaag acggcgctgc ttttttccct aaacttataa aagatttcca
    10261 acttgatcaa acgatttacg cacaatcagg gacacttttg cttcgacctg aaaaagattt
    10321 agctgaattg gctgttcttg ccgagaaacg aaaagaagaa gcgccagaaa ttggaaacat
    10381 cgaatggctt tcacctgaac agacatctca agcacttccg ttattgaaac cagcagtttc
    10441 actgcgtatc agtggtggtg gtcgtctgga tggaaaagca tatttagaac atcttggtca
    10501 gcgtttagca caaaaacaaa tggagcagat ccaggcaaaa gtcactttgc ataagaagag
    10561 cgattcttgg ctcatcaagg gaccgtttgg agaatacaca gctgatcgtg taattttgac
    10621 acctggcccg gcacttaagc ctttattaca agaattaggc tatgctgcag atatacgtcc
    10681 gcaaaaaggg cagctcttag catttcagac atcttataag atgagtcaag actggcctgt
    10741 tgctatgctt gacggagaag ctgatttcat tcctttcatg gatggaaaga tccttttagg
    10801 tgctacccat gaaaacgatc aaggctggga tttggctcct actagcgaag cttataaggg
    10861 attgactaag agtacagaac agttcttgac tgatccagat cagataaaaa actggccata
    10921 cgagttccgt gtaggtaccc gtgcttatac gtcagatttt tccccatttt tcggcgtact
    10981 gccagatgat ccaagcttac tggctgctag cggtctaggc tcttctggct tgaccacagg
    11041 tccttatatc ggctatctat tagccaactt cttaaatact ggtcattggt ctacggaaga
    11101 ttatcaaaag gacatctcca cctatattac gaaaaatcca tcatagaaaa acagcttttt
    11161 caagtaagga tcccgagcga tattgcgcat ggattacttc ttgaaaaagc tgttatttta
    11221 gttcaatttc tgaatcaaat tctccaaact ttcttttgct tcatctggca agagatcttt
    11281 tcctatctga gcttcaatga atcgtaaacg aatatcaagt cgttgtttca ccagattttt
    11341 ataggacgta tcctcaaaag aaacaggaaa atctggtata ggtaaatagt ggatgtcttc
    11401 aaatggacca aacgacttga attctgccgt ttgttgcacg attctttcaa cgattgacaa
    11461 tgtcgtttca ggtttgattt ttttatcatc gaaatatcca gtattcagga catagcaggc
    11521 tgtttgctgt ttcaaaaaca actctcgaaa agcttgatag tcttccctaa gaggatagac
    11581 acggaaagga ttagcaaatg gttcaatgac tagctcatct agctgtcttt ttgtagtgat
    11641 attttccgcg tctgatcgtt ttgttgctaa tgttgcacca aaagacgctg ctaaaactgg
    11701 gtcattgatc ttgataattg gcggaagcga atcatctttc atgatccaaa aaacagcatc
    11761 gatcttttcg ctaagatgat cgactcggtc gggtgtcgca taacgtgatt tgactgtccg
    11821 cccatttccg tttcgtatat ctcctgttac taggactttt tctcctttac ttgtacatgt
    11881 cacgcccaca ttctgacaag taaggaaaga agaaatggcc ggatcatcag acggatagtc
    11941 ttgtgtttta tcaaagtaag aaggttccaa ggcagtagca aaaccatttt ttcgagaaat
    12001 aacgaaagca tcgtcatgca agatcgaaac agagtgacct tccgtatgtt ttgctaaggt
    12061 aatcgttgat tttccagaac cggacaaccc aaatacagcc atcgtatagg tctggtcatt
    12121 actagcatat tgtttcatcc caccgtggca ggcaataaag ccattgcgat ttcccaatgc
    12181 ccaagcaagc gtcaaagtcg ctttttttaa ttctccaaag taacgtaatc ctagcacagc
    12241 cgctgtatta tggagagcat caaacaaaat gagtccattt ggaaatgttt catgtgtcca
    12301 ttcgggatca caaaagatat aaagatctcc ctcatcaaag gaaacagact tttgataagt
    12361 ctctaagatc ttcgaattcg ctggctgaaa attcagcaaa taagaataca aattgttctc
    12421 aaatccttct ggaatcagca aatgactttg cacagaaaat tcttcagata ggccaacgat
    12481 tacttttgta tggtaaaact ggcgcttgcg actttgatac acagcttctc ttaaaatttt
    12541 ctcgtatttg ctttgttcct tgtctaagtc ctcaaagatc cgccgagcac ttgctgttct
    12601 tcccacaatg cttccatcat tgaatactag tgtcctaaca tcagctggta aatctagttc
    12661 ttcagcatgt gagattgtta aatctgtgat gatcgttcct ggactccctt ttgctagttg
    12721 ataagctgtc ttcctgtcaa aaacttcatg aacattatta ccgtaaaatg tcgtttctac
    12781 aatcgatcga atactggata acagtggatt tccacttgtg atttcgttta attcgagact
    12841 ctcttttgta ctcatggctc ctcctcattc cctaaacaaa aaaacgtttt ctttattata
    12901 cattattcga gtttttattt gtactgctgc gctgtgggaa gcaaacgagt tatttttcac
    12961 acaaaaaaac cgagaacttg aataaacaag tttctcggtt tcatcatttt ccttttatcg
    13021 gcttagtacc agccgtttgc taaccagaat gttttagcag catcccatga accataacga
    13081 gatgttacat attgttctgc tacacgttct tgattggcag ctgaatagtc gccgtttaag
    13141 tatgaagcat ctaattgata acggccgatg taacgtccgt tagtagctgt gtatgaaccg
    13201 cctgattctt tttgtgcgat ccattctttt gcagagcttg aagttggcgc tgttgcagca
    13261 gctggggcag caggagctac ttcaactgtt tcaacaggtg caacgtatgt atgttcttgc
    13321 gttgctgttt cagttgtaga tgctgtattt tctgttgttt tgttttctgt tgttgctgaa
    13381 gaatcatttg aagttgggat tgttaattgt tcaccaacat aaatgcggtt gatgtttgcg
    13441 atcgaatttt tttcagcaat tgcatcgatc attgagttgt ctcctgcaaa tttttgtgag
    13501 atcttagata atgaatcacc tgattttact gtataaactt catcagcgtg agcatttgta
    13561 gttcctacaa acattgcagt agcaccagca gcaaaagttg tacctaaaac aattttctta
    13621 aatgaattca tattaaactc ctttttttct ccgtttttta ttctttcgac gctattaact
    13681 ttatcattta gaaatatttc ataggtaaaa gaaacatgac atttaatgac aaaataacga
    13741 attatttaat atttgtaaca tttaaacaaa tatttttttg atacaaaacc ataaaagcct
    13801 tgttgtataa gcaaagtaat cgtttttcta tgttttttta tttttgaatg aaaaacgcag
    13861 att
//
LOCUS       DARBDV010000076        13810 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000076 DARBDV010000000
VERSION     DARBDV010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.077"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(221..1423)
                     /locus_tag="SCM56_002165"
     CDS             complement(221..1423)
                     /locus_tag="SCM56_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1110915.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1426..1620)
                     /locus_tag="SCM56_002166"
     CDS             complement(1426..1620)
                     /locus_tag="SCM56_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEG1110916.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1667..2515)
                     /locus_tag="SCM56_002167"
     CDS             complement(1667..2515)
                     /locus_tag="SCM56_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HEG1110917.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2720..3070)
                     /locus_tag="SCM56_002168"
     CDS             complement(2720..3070)
                     /locus_tag="SCM56_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110918.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3665..4111
                     /locus_tag="SCM56_002169"
     CDS             3665..4111
                     /locus_tag="SCM56_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HEG1110919.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            4735..5727
                     /locus_tag="SCM56_002170"
     CDS             4735..5727
                     /locus_tag="SCM56_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1110920.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5743..6627
                     /locus_tag="SCM56_002171"
     CDS             5743..6627
                     /locus_tag="SCM56_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1110921.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6676..8283
                     /locus_tag="SCM56_002172"
     CDS             6676..8283
                     /locus_tag="SCM56_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1110922.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8284..9768
                     /locus_tag="SCM56_002173"
     CDS             8284..9768
                     /locus_tag="SCM56_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEG1110923.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9945..10937
                     /locus_tag="SCM56_002174"
     CDS             9945..10937
                     /locus_tag="SCM56_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1110924.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10989..11858
                     /locus_tag="SCM56_002175"
     CDS             10989..11858
                     /locus_tag="SCM56_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG1110925.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12278..13771)
                     /gene="guaA"
                     /locus_tag="SCM56_002176"
     CDS             complement(12278..13771)
                     /gene="guaA"
                     /locus_tag="SCM56_002176"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEG1110926.1"
                     /translation="MITRRIREFGVFSELMSHRITAEEVKAMNPKGIILSGGPNSVYD
                     EKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGKAELEITSAEAALF
                     QNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKMYGIQFHAEVRHSE
                     YGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLGLSGGVDSSVVGVL
                     LQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAKERFLSKLAGVSDP
                     EEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGTETAQTIKSHHNVG
                     GLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPGLGIRVLGELTEEK
                     LEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRTYDYTVGIRAVTSI
                     DGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVEWE"
BASE COUNT         4231 a         2337 c         2851 g         4391 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttacgtt gaaaaacgtt gatttatcgg cattccccat ccatattata
      121 tttacgtctt attcccactc aatatgagta gcctcgtaaa gaagctatta tatcagtgct
      181 ttttcagcct attcctcatt ttggtaggga aatggtaggg ttagattttt actgcttgat
      241 ttccattaaa gtttgcaact ttagattttg ttgcacaggt aaatttaatg tttcctgtca
      301 tttgttctgc aattccttca tcattacggc tccatagatg agcgtagtgc tttaaagtga
      361 tttcagggct agaatggcct aaccgttgcg acacgaccag aatgtctgcg ttaaactcat
      421 tgattaggta gctaacatga gaatgacgta atccttttcc ttgtattagg ggtaccttag
      481 ctagtttggc ataacgctga ataattcgtt gaacagttga acgatagagt gggaggtcag
      541 tgtagctaag aataaattgt tctacaccat gatttttttg aataactttc cactttttta
      601 gcacttcaat tgtatcatcg tccagagaaa tgatccgcaa gccgctttca gtttttgtgt
      661 aaggtttccg aataaaatct ttttgatttg tcatttccaa ggtgtgatga accctcaact
      721 ttttacgttt gaagtccacg tcattccaat taagtgctaa tccttcgctt acacgaacac
      781 cagtcatgta atatagccag atcattaaaa aacacatatg ttcgtagaaa tcatctttat
      841 aaatttttgc caaaacttct tcaaattctt cttttgtcca gtaaggaacg attgattttc
      901 cttttggaat tgcttttgtt cttttggaaa tattctcatt cagaaattgc agggtgaccg
      961 catagtctaa tgatttacga aacataccgt acataagtga actgtatgct tgtgagtatc
     1021 cgctatcgtt caataaccaa attcgataat tttcacaatc tcgcacattg atttcacgaa
     1081 gcttcttatc accgaaaaat tcagtaatct gaatcaatcc acactcacga gaactccaag
     1141 tacttttttg tacgctagct ttgtagtgtg gaacgtaaga ctgctccata aattctttgt
     1201 aagttaaatc ataattcgca aatccattta caaccatgta ttcatttttt atacgcatga
     1261 cttctttgtg tgcttcacga gctgatgaga atcgttttcc tagagaatct tttgttcctt
     1321 tcttctggat cctctttcca gtcacttgat ctgttcctaa ctcaactaga taagaaaagc
     1381 gaccattttt atcttgatac acacaagggt attttgtttt catagttatt cctcctgtga
     1441 aagaggaaca cctataattt cttcaattac ttgtcggggt actaccccaa ctcttttatt
     1501 attataatat ggatatccct gatttaccat tcttgttttt gcttgtcgga ttattgtggt
     1561 agcttgatgt gttttaaatc ctagagcctc taaatctttt cgacaaattg tatttttcat
     1621 tgtataaact cactttcaaa gaaaacacta tcattgggta atagtgttat ttctttattt
     1681 ttgtataatg aacggtactt tcttgaaaat ccatcacttg ttcaggactt cccaactcat
     1741 ttaaagctaa agcattgtaa aaaaatggac gtctaactat atattcgctt gtatatagta
     1801 tatcttgatt cgtggaatca tagggtttct ttaccatctt tttggtgaca gttggtagtt
     1861 tgagattccg ggacttgaaa aaagcttttt tcttatgctc tttcaattcc agctctttat
     1921 cgaaatattt gctgatatag cgcccgcgat tttcgatact gtctacatcg atcttgttga
     1981 tcttaacgaa accctgaccc caaatcgtag ttaaccggtt aaatgagata aaggggaaat
     2041 caaacaagat aatatggtag tggattgcac cgcgttgctg cttttcccac gtggcgagat
     2101 actttaaatt tgctttttta gttttataga ggtcataatt cagtcgtttg ataaattttt
     2161 taaattcatt atttgcatac tcaatgtctt gaatgttttc tttgaaagtg agagttaaaa
     2221 atttcgtttg attgtcaaaa ttcgtgtcta caaggcgggc aatctcaaat cgcatacttt
     2281 gataatgttt ctgtttgcgt tttaaactgt catattgtcc ttgagcggat aattcatcat
     2341 agttttttct tttttcaaga gtaatatcat tttcataaat ccaagctaat ttttgttttc
     2401 gttccagatt tacttcgttt tcgatcgttt tacgtttggg agtaatgatt gcttcataat
     2461 cataaatttc aatatacgtg ggagtttcaa tgattttctg attataagct tgcaaaaatc
     2521 atcactcctt gttgcgtatt cgattgtcaa agaaccagtc attttctcct gatgttgatc
     2581 tataaatcaa gttaaggggg aaggcctgcg tagcagccct tctagcctat ggggtgttgg
     2641 gcggtcttaa cggacagaaa cggctctgtg ggcgctgccc cgccgtttcg tcctaaccac
     2701 ccaacactcc atagtatagt tagttttgtg tagtggtacc ttgttttctg ggaatcaaag
     2761 ttgccgtatc agcggttgcc gataacccat aactgacaaa ttgtccattc gagcgtcccc
     2821 aaggtacaat caccaaattg gtaaatgtaa ttatggctaa accttgttgg gtaagttctt
     2881 ccggcgtgat cacaggatgt tcagatttga ctttgacact gattttttcg tagcgaagtt
     2941 ttggtaggat acattcgtag ctccaaccta gaatttcgcc ctccttttgc cagcgagtga
     3001 cattgacgac ttggtattct ttttcgagtg tttcttttgg taaactgaga tcggtaattt
     3061 ttattggcat cacaattctc cttttttata cgaaagttta tacgatttga ttaaaaaata
     3121 tagatggcca tctacgactt gtattatata cgagaactta tacgtagtca agagattttc
     3181 taaaaaaata tatacgattt tttatacgag tgttataatg aaaaaaatga taaggaggaa
     3241 tttgaatatg tttggtttag tacaacctaa taaagtgaag gtgggccaac gaatcaaaga
     3301 aataaaagac ggaatgtctc tctcttttac agaattaggg aatcgattag gtttaaaaaa
     3361 gccgacgatc agttcctacg ttcaaggcta tgcgttggct cctaagagtg tgatcaacca
     3421 attatctagt atcagcggaa aacctgtcgg ctggttttat tttgggcatg tggaagaata
     3481 tatccgagag tatttgtttc tcattggtca gaaaaaaata gtggacgatt atcctgaaat
     3541 cattgagaaa ataaagcaag aattttatac gggcgagttc aaaaatcctg cttgggagaa
     3601 tgagctaggc tatcctttgg aggaatttat ccaagaatgt tttgtagaat attcgagtga
     3661 aatcatgaag gactatgtcg tacagattac gaaagaggta cttgaagaat ccaaaacgct
     3721 aaacaaacta tcatcaaaag caaaagaaga agctgtcacg attctttcat ctgggatcat
     3781 tgactatatc gagatgagtg gtgagatcaa gtatggagag aaagatcgca ttgcgaaaat
     3841 ggtgaaagaa tcaataaaaa cctttgatgt gaaacaagac ctgacattta atgattccta
     3901 tttgattgga aagttgatca atattcttgg tacagatgaa gatacggagc tgtttctttc
     3961 ttctttatca atgatattta ctgataagca tttttccact cgatttggtg gggaagaatt
     4021 agtcgaaata tttcaatcga tgcgaccagc gttaatcaaa ctctatcggg agaaaacacc
     4081 agatgaattt tatgattggt ttgagaagta aaataagaaa agtggcttag attacaagtt
     4141 ggtgaaaaaa tttttttcag cataacatca gtaatctata aaggggttgt tgattatact
     4201 gtagaagtta agtactcaag agattttttg tacgtaaaca aagcaaaaaa gggtgcagtt
     4261 tcactagaaa atgtactctt ttttgtgatt tttcttagtt atagagtttt tttgaaactt
     4321 taaaaatgag aaaacatatt gattttaaag agtgtagaca tagcttcttt tgctaaatag
     4381 attaaactta ctggtgagtg gtcttgaaca tacttttaaa tcacatttta ctgagtttta
     4441 ttggatttta taattcattg gaaacctcgt acttaatctg tttttatcta tcgactagaa
     4501 tctgtatata ttccagctat caatgaggcc gcagaaaagg gtgcttaaaa ataatctgtt
     4561 gtttaaaata ttattatcag aatacttaga tacaaaattc tggcacaaag taaaaaaaat
     4621 attgacttaa aaaaatggat atggtaatct ataaacgaaa atagtggtaa cgtttccaaa
     4681 atgcatcttc gttaaatttt tattttttta aacgaaaatg gaaacgtttc taaaatgtat
     4741 tgggaggaga ttgaattgga aaagacattg ggaccaggtg gtgagataag ggcggtacaa
     4801 cgggataaaa aaaatatttc gaagtgggaa aaattaaaga aacagttgcg gcgtgataag
     4861 tggatgtact tgttgctctt accaggtatc atcatgacgt ttatctttaa gtatgtgcca
     4921 atgtacggtg ctgtgattgc atttaaagac tataatccta tgctaggaat tttggaaagt
     4981 ccttgggtag gactagatca ctttagggaa tttatcagtt caccaaattt tacgcttttg
     5041 ctgagcaaca cattaaaaat tagtatttat ggtttattgt taggtttttt tccaccaatt
     5101 attttagcac tgagcttgaa tcaaattatc aataaaaaaa taaaacagaa aatccagttg
     5161 attctatatg cgccgaattt tatctcagta gttattattg tggggatgct gtttttgttc
     5221 ctatccgcaa atggacctat taatgggtta gtagaaaagg ttacggggca accaattatg
     5281 ttcatgtcta atccagctta tttcagaacg atttatattt tatcgggaat ttggcaagga
     5341 atcggctggt catccattct ttacaccgca acgctggcgg gagtcagtac cgatttatat
     5401 gaggcagcaa atatcgatgg ggcaaatatt ttacaaaaga tttggcatat cgatttgcct
     5461 gcattgaggc ctgtgatggt gatcagcttc atcttggccg ccggcggtat tatgaatgta
     5521 ggatatgaaa aagcctactt gatgcaaact tcaatgaaca tccctgcctc agaaatcatt
     5581 tccacttatg tatacaaagt cggcttgcaa tcaggtgatt atgcctactc tgctgctgtt
     5641 ggtctgttca atatgattat taatgtcgtt ttattactga ttgtcaacaa tataaccaag
     5701 cggctaaacg atggtcaagg cttgtaatga aaggagaaaa ttatgaatag tgctttatca
     5761 aaaatcgacc gtcaaatctt atggttgaat cgagtgattg taactttttt agtcgtcatc
     5821 accattgttc cgctgattta tgtactggta gcttctttta tggacccagc tactttgatc
     5881 aataaaggta taagcttcaa tccgaaagat tggacaatac agggatatca acgggtattt
     5941 gcagatgatt ccattttacg cggttttgta aattcgttgt tttactcttt tagcttcagc
     6001 ttattgactg tatttattac gatgatcact gcgtatcctt tgtctcgacc taacttggcg
     6061 gggaaaaaga ttattatgac tttttttgtc attacaatgt tcgtgggtgg tggcttgata
     6121 cctacctatt tattggtaaa gaacttgggc atgttagata gtgtatgggc gattatcgta
     6181 ccaggtgcta taaatgtgtg gaatattatc ttagcccgta cctattttag tgctttgcca
     6241 aaagaacttt ctgaagctgc tactatcgat ggcgccaatg acttgcaaat actgttcaaa
     6301 gtcatgcttc cattagcgaa accaattatg ttcgtattgt ttttatatgc ctttgttggg
     6361 caatggaact catactttga tgccatgatt tacatcaaag atcccgactt acagccgttg
     6421 caactcgtat taagaaatat tttgatccaa aaccaacctt cacaagacat ggtgggagcc
     6481 aatactgcca tggcagaaat gaaacaaatt gctgagttga tcaaatacgc aacgatcgtc
     6541 atttctagtc tgccattaat cataatgtat cctttcttcc aaaaatattt tgataaaggt
     6601 gttttagttg gttctgttaa agggtaagta ttgttcacta tttgtatgaa taaaaattat
     6661 aaaggagatt ttaagatgaa agcaaaatgg atgctgccaa tcgtttttac ttccgtacta
     6721 attttggggg cttgtggagg ctcaaaagaa aagagtaaat caagccctga ttatgaacta
     6781 gaaaatgtct cttttccatt gaaagaaaaa gtatctttaa aaatgatgtc tcaaagtgcc
     6841 cctttggctc ccaatgatcc taatgacaaa ttgattttcc aacgattaga agaagagacc
     6901 aatctccata ttgattggac aaactacagt agtgattttg ccgaaaaaag aaatttggat
     6961 atcgctagtg gtgatttacc agatgccatc ttcaacgccg ctgcaggtga ctatgattta
     7021 ttaaactggg cggaaagtgg cgttattgta ccgattgaag atttgatcga agattatatg
     7081 ccaaatctga agaaaatttt tgatgaaaac cccgaatacc gacaaatgtc tactgcacct
     7141 gatggtcaca tttactctat gccttggatt gaagaattag gcgaaggaaa agagtcgatt
     7201 catacagtaa atgggatggc ttggatcaac aaggagtggt tggataattt gggtcttgaa
     7261 atgccgcaaa ccactgatca gttgatgact gtgttggagg ctttcaagac gcaagatcca
     7321 aatggtaatg gtgaagcaga tgaaattcct tttagcttta ttaacaatgg aggaaacgaa
     7381 gacttaaaat ttttaatggg ggcattcggt ctaggtgata atgatgacca cttagtcgtt
     7441 gataacgatg gtcaggtaca atttactgct aacaaagaag ggtataaaga agccatcaaa
     7501 tatttcaatg aaatgaataa taaaggtctt attgataaag aggctttcga acaagattgg
     7561 aatacgtatg ttgccaaagg gaaagaacat aagtatggcg tgtactttac ttgggataaa
     7621 gcaaatatca cgggtgcaga tgatagttat gacgtcttgc cagtcttagc gggaccagat
     7681 ggaacgaaac atgttactcg tacgaatggc atgggtttct caagagatcg tttcgtaatt
     7741 actagtgcga ataaaaactt ggaacttaca gctaggtgga tagataaaat gtatgaaccg
     7801 ttacagtctg tccaaaataa ctggggaacc tacggtgatg aagcacaggc gaatattttt
     7861 gaattaaagg atggtatgtt aaaacatttg cctttagagg ggacagcacc gggggaacta
     7921 agacaaaaaa ccgaagctgg aggaccatta gcagttcttg attcttatta cggcacagtc
     7981 actactatgc cagatgatgc gaagtggcgt ttagatttga tgcatgacac gtatttgcca
     8041 tatattacag aagacaatat ctatccacgt gtctttttag aaaaagaaga aacagatcga
     8101 ttagcaaaaa tcgaaacgga tatgtgggat tacatctatc gcaaaagagc agagtggatc
     8161 accggcggta aaatcgatga agaatgggaa gaataccttg ctgaattgga acgtgtaggt
     8221 ttggaagaat ggattcaaat aaaacaaact ggctatgata attttttgaa gggagcacaa
     8281 taagtgttag tagaagaaac ggcgattttg gataaagcaa atcgatttat tgaaatgatg
     8341 cgaggaaaag ttaaccctca gtatcgacaa aactatcatt tttccgcacc catcgggtgg
     8401 ataaatgacc ccaatgggtt tatatattac aaagggcatt accatctctt ctatcaatac
     8461 tatccttacg atagtcgatg ggggcccatg cattgggggc atgcgcgcag tagtgatttg
     8521 gtccattggg aagatctacc agtagcgtta gccccagatc aaccttatga cagcggcggg
     8581 tgtttctcag gaagtgcaat tgaaagagac ggaaaactat atttatttta tacaggctgt
     8641 caagaaattg atggtattag ccatcagcat caatgtttgg cggtctctga agatggtatt
     8701 cattttagca aatacagtgg taatcctgtg gtagcagaaa aacagctagg tgaaaatggg
     8761 ctaatacaag aatttcgtga tcctaaagta attgttcaca acgatggtta ctatatggta
     8821 gtagctacta aatctacaca gtctactggt agaatagtga tgtttcaatc gtcagatttg
     8881 tatacttggc acttttactc tgtgctgcta gaagggactt cagagcaagg aataatgtgg
     8941 gaatgtcctg atttattcca tttagacgga cgagatgtct taatcatgtc tccgatacaa
     9001 atcaagaaga acggactgga atatcataat atcagttcta ccatggcttg tattggtgag
     9061 gtaaactggg aaacaggtca gatgatagtt gataatttcc atgaaatgga ttttggaatg
     9121 gatttttatg caccacaaac aacaagagat cctaatggtg gacgggtgat gattgcttgg
     9181 atgcagatgt gggatcgtac attgcctaca catgagttag gccatctttg ggcaggctct
     9241 atgagtttgc cccgagagtt aagagtgaaa aaaaatcgat tggtacaaaa acccatctca
     9301 actgtatata gtcatttagt ttatcaacaa ggcagtgaaa atattgcagt gactgacaca
     9361 tcagtcgtat ttcgaaacgc gctgtctgat tcttcatatt tacatctagt cgcagatttg
     9421 gagaaagctg aagtttttac aattgaatta ctgcggtcta agacggattg tctcaaacta
     9481 gcctatgaca ctgctacaca ggtttttgaa ttggatcgtc agaaactagg aagagagctt
     9541 acaggggcgg aagaagaccc tctgaatgta cgaaaagttg aagtaccttt gattgataat
     9601 cagctcgttc ttgagatttt tagagatacc tcatcaattg aagtctttat taatgggtta
     9661 gaagttatga cggctacttt ctatgagctg gaaaaagctc gagatatctt gtttcattcg
     9721 atcggagaaa gtcgtattca agcttttgaa acagcgagtg tgaaataaat gtttgattgc
     9781 ttaggcgatg aatcaattat ttaataggtt ttgaaggata atgtttttag aaaagaacag
     9841 atagctagat ttcccatgga tttgctatta gtattttgaa aaaattagta atttatgaca
     9901 aaattcatta tactaaactt gtgcagaaat gtgagggatc aactatgaaa gcaacaatga
     9961 aagatgtcgc aaaattagca ggtgttggag taggaactgt ttcccgggta ttgaataacg
    10021 gttctgttaa agcttctact agagaaaaag ttgaagcagc aatgacagag ttaaactatc
    10081 aaccagattt ttatgcccgg gggttaaaga tgaagcatac gaacacggtt gctttgattc
    10141 tacccacggt atggcatcca ttttttggcg aatttgccta ccatgtagaa acagcattag
    10201 aaagacaaaa ctacaagctt tatttgtgta actccagtgg taatagtgaa aaagaacaag
    10261 aatatatcca gatggtcagc caaaataaag tagacggcat cattggaatt acttattcag
    10321 atatcgatga atacatttcg agtaatctgc cttttgttag tattgaccgt tatttcactg
    10381 aagaggtgat ttatgtgacc gctgacaatg cggccggtgg acgtttggct gcaaatgaac
    10441 tttatcgccg aggatgtcag aatgtagcat atatcggtgg gtaccaagaa acaccgaatg
    10501 aaacgaagag tcgtcgaaaa tattttgaaa tcgagtgccg tcaactgggg atttcctata
    10561 aagcgttaga tatgttagaa cctttagacc atctctcaga acgtgtaaca aattttttgt
    10621 tagagaatcc cgatatcgat gggattttta cgatcaacga tccaatggca ttagctgtca
    10681 ttgatatgct tgaaaaaata gggaaaaacg taccagagga tgtccaagtt atcggatttg
    10741 atggtcaaaa gatgacagaa ggagatcctt atctagtctc aacgattgcc caacctacaa
    10801 agcaaatggc ggaagtggca gtggcaaaat taattgctgt cattaatgga gagacagtag
    10861 aaaaacgtac cgtattgccg attgaatttg gcgaaggtga cacaacgaaa cctttagtga
    10921 gaaacgattt tgcttagtgc aaatacgttt ctttttttga aaatgagtga atgaaaggtg
    10981 tagataaaat gttatatggt ggaatagaag ccggtggaac aaagtttgtc tgtgggattg
    11041 gtaatgaaca attacaagtg atagaacgga aaagttttcc taccacaaca ccagaagaaa
    11101 cgatggagca agtgtttgat ttctttgata agtacaaagg aaatatcgca tcaattggtg
    11161 taggagcatt tgggcctatt gatattcaaa tcgaatcaag aacttacgga tatatcacca
    11221 acacaccgaa gttagcttgg caaaattatc attttgtagg agctttggaa aacgccttgt
    11281 cggttcccat ctattggact accgatgtaa atgctgcttg ttatggagaa tatatggcag
    11341 ggtatggaaa aggtaaaaat agtgttctgt attacaccgt tggtacaggt gttggtggtg
    11401 gtgctgtagt ggatgggaaa atagtcgcag gttttagtca tcctgaaatg ggacatatgc
    11461 tcttacaacg tcataatgaa gatcaatttg aaggaatctg tccttttcat catgattgtt
    11521 tggaagggtt agcatcagga cctgcgattg aaaaacgctt tggaagaaaa gcccagacta
    11581 tctcatttga tcatccttat tggacgattg aggcagatta tcttgctcag tgtgtgtata
    11641 acacaacgtt actatatgca ccggaagtaa taattttagg cgggggtgta atgaaacagc
    11701 cgcaactact cgagttgatt cgccaatcgt ttaaagaaaa aatgggttcc tacgtggata
    11761 cgcctactct cgaagaatat ctactaactc caaggcttgc tgacaatgcg ggcttgattg
    11821 gctgcctagc tttagcaata aagaaactca atcattagaa tagtatttat tagaactatt
    11881 cataacatac cggaagttta aaagggtatt ctgaaactta aatagtctca attcattctt
    11941 ctttcttgag aatctgagta gagactttta ttctgtttca aattaatata attacgaaat
    12001 gatattgagt acaagaaagt tctttttacc agatctttta aatcgaaaaa tctgatatat
    12061 atatttatag tactaaaaat ctgattagat aaaaagctaa ttcaatagga aaataaaggt
    12121 agtgaataaa agcaaagccc ttgttagtag ttaatctaac aagggctttg gtagggaaaa
    12181 tggtaggaaa ccatcttatt ttttacgaaa atcaggaact ttagtaattg aataatgttg
    12241 atttataggt attcctcatc catattatgt tggctcttta ttcccactcc actgttgcag
    12301 gtggtttgct ggtaatatca tacacgatac ggttaacgtg tgcgacttca tttacgatac
    12361 ggacactgat tttttgcaag acttcccaag ggatacgggc aaagtcagcg gtcatgccgt
    12421 caatagaagt gacagcacgg atacctacag tgtagtcata cgtacggcca tctcccataa
    12481 ccccaacaga gcgaatgcct ggtaggacgg tgaagtattg ccagatgtcg cgatctaaac
    12541 cggcattggc gatttcttcg cgcaagatag cgtctgattc acggacgatt tctaattttt
    12601 cttctgtcaa ttcaccaagg acacgaatac ctaagcctgg tccagggaat ggttggcgcc
    12661 aaacgatgct gtcaggcatg cctaattggg tacctaattc acgtacttcg tctttgaaca
    12721 atgtgttcaa tggctcgatc agcttgaatt gcatgtcttc aggtaatcca cctacattgt
    12781 gatgtgattt gatggtttgg gcagtttcag ttcctgattc gatcacatcg gtataaaggg
    12841 ttccttgtgc aaggaaagaa acgccttctt caccggcaag tttggttgct tcatcatcga
    12901 agacatatac aaattcattt ccaatgattt tacgtttttc ttctggatca gacacgccag
    12961 caagttttga taagaaacgt tctttggcat cgacttttat aatgttcaag ccaaattttc
    13021 cgccaagact ttccataact tgttctgctt cacctttacg caacaatacg tgatcaacga
    13081 agatacatgt taattgatcg ccgatcgctt tttgtaaaag aacaccaaca acagaggaat
    13141 ctactccgcc tgaaaggcct aataaaactt ttttgtcacc gacttgttca cgaatttttg
    13201 cgatctccat gtcgatgaag ttatccattg tccagtcacc agtacattgg cagacatcta
    13261 gtgtaaaatg gcgcaacaag tcatttccat attcggaatg gcgaacttct gcatggaatt
    13321 ggattccgta cattttgcgt tctgcatttt ggatcgatgc gatggggcaa tctttgctgg
    13381 tagcaacgac atcgaaatca gtcggtgctt gggtaactag gtcaccatga ctcatccaaa
    13441 cggtttgttt ttcaggagtg ttttggaaaa gggccgcttc tgcagaagtg atttccaatt
    13501 ctgcttttcc gtattcacgg tttttggctg gtttaacttt tccgcctaat ttataagtaa
    13561 tcaactgcat accgtaacag attcctaaaa ctgggatccc caaatcaaaa atcttttcat
    13621 caataccaaa ggccttctca tcataaacac tgtttggtcc gccagaaagg atgattcctt
    13681 tagggttcat tgcctttact tcttctgcgg taatgcggtg gctcatcaac tctgagaaaa
    13741 caccgaactc acgaatacgg cgagtgatca gttggttata ctgactaccg taatcaagta
    13801 cgataatttt
//
LOCUS       DARBDV010000077        13638 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000077 DARBDV010000000
VERSION     DARBDV010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.078"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            65..307
                     /gene="yaaA"
                     /locus_tag="SCM56_002177"
     CDS             65..307
                     /gene="yaaA"
                     /locus_tag="SCM56_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEG1110927.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            294..1418
                     /gene="recF"
                     /locus_tag="SCM56_002178"
     CDS             294..1418
                     /gene="recF"
                     /locus_tag="SCM56_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEG1110928.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1415..3361
                     /gene="gyrB"
                     /locus_tag="SCM56_002179"
     CDS             1415..3361
                     /gene="gyrB"
                     /locus_tag="SCM56_002179"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEG1110929.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3381..5852
                     /gene="gyrA"
                     /locus_tag="SCM56_002180"
     CDS             3381..5852
                     /gene="gyrA"
                     /locus_tag="SCM56_002180"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEG1110930.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6088..6387
                     /locus_tag="SCM56_002181"
     CDS             6088..6387
                     /locus_tag="SCM56_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEG1110931.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6437..6949
                     /gene="ssb"
                     /locus_tag="SCM56_002182"
     CDS             6437..6949
                     /gene="ssb"
                     /locus_tag="SCM56_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEG1110932.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6975..7211
                     /locus_tag="SCM56_002183"
     CDS             6975..7211
                     /locus_tag="SCM56_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEG1110933.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7354..9327
                     /locus_tag="SCM56_002184"
     CDS             7354..9327
                     /locus_tag="SCM56_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEG1110934.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9333..9785
                     /gene="rplI"
                     /locus_tag="SCM56_002185"
     CDS             9333..9785
                     /gene="rplI"
                     /locus_tag="SCM56_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEG1110935.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10059..11426
                     /gene="dnaB"
                     /locus_tag="SCM56_002186"
     CDS             10059..11426
                     /gene="dnaB"
                     /locus_tag="SCM56_002186"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEG1110936.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12206..13498
                     /locus_tag="SCM56_002187"
     CDS             12206..13498
                     /locus_tag="SCM56_002187"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEG1110937.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         4545 a         2467 c         3001 g         3625 t
ORIGIN      
        1 gttttattgc gaaaaaaaag gtataataga gtatactttt aataatgaaa atgagggatg
       61 ggcattgaaa caacaagtca ttctaaatac ggaattcatg acgttgggcc aaatgttgaa
      121 agaagtcaca gttatcggta gtggcggaca agcaaaatgg tatttggcag aaaacactgt
      181 gttagtcgat ggcgaaccag aaaatcgtcg tggacgcaaa ctttatcccg gcatgatggt
      241 tgaagttcca gaagtaggaa ctttttttat ggtaaaaaaa ggaaacgacg ataatgaggc
      301 tgaataagct gtatttaaaa aattatcgaa attacgaaga gctgaacatc gaattttcca
      361 agaacctcgt catcttttta ggagaaaatg cgcaaggaaa aacgaatctt ttggaaagta
      421 tctacgtatt agctatgacg cgaagtcatc ggacaagtag tgagcaggaa ttgattggct
      481 ggactgacga tcaggcgatg attcaaggag agatcacaaa aggttcatcc actcttccat
      541 tagaaatctt attatctaaa aaaggccgaa aaaccaagat caaccatatc gaacagaaaa
      601 agttaagtac ttacgtggga caaatgaatg tcattttatt tgcaccagaa gatctttctt
      661 tagtaaaagg cagcccgcag accaggcgga agtttttaga tatggaatta ggccagatca
      721 atccaatcta tctttatgat cttgtccaat accaagcaat tttgaaacaa agaaaccagt
      781 acttgaagca attgaatgaa aaaaaacaaa ctgatttgct ttatttggat attttgtccg
      841 aacaactagc agcgtttggc agcaaagtat tgaaagcaag agcacagttc gtcaagcgat
      901 tggagttttg ggctaattct ttgcatcagc aaatcaccca tcaaaaagag cagctggaaa
      961 tcgagtatct caccgcagtc gattctttag aaacacatac gcaagagcaa atccaagaac
     1021 aatttctcgc acttttgaac caaaataaaa agaaagacct ttttcgggga acaacgacag
     1081 ttggtcctca tcgtgacgat ctttcctttt ttattaatca gaaaaatgtg cagacttatg
     1141 gatcgcaagg acagcaaaga acaactgcac taagtgtcaa attagcagaa atcgatttga
     1201 ttaaagaaga gacaggggaa tatccaatcc ttttgttaga tgatgtgatg agcgagctgg
     1261 atgataatcg tcaactccat ctgctagaaa caatcgaagg aaaagttcaa acgttcttaa
     1321 caacaacgac acttgaccat gtaaaaaata aaatgactgt tgaacctgag attttttatg
     1381 ttcaacaagg gaaactaaaa ggagaagaaa ctgaatgaca gaagaaagaa gtttagtaga
     1441 acgcgctaaa gagtatgatg ccagtcagat tcaagtactt gaaggacttg aagccgttcg
     1501 taaacgacct ggcatgtata tcggttcgac tagttctgag ggtctgcatc accttgtttg
     1561 ggaaattgtc gataattcga tcgatgaagt attagcaggt tttgcaacga aaatccatgt
     1621 gattattgaa aaagacaaca gtatcaccgt tattgacgac ggtcggggga tacctgtaga
     1681 tatccaggca aaaactggac gtcctgcagt tgaaacggtc tttactgttt tacatgctgg
     1741 aggaaaattc ggcggtggtg ggtacaaagt ttctggtgga cttcacggag taggatcttc
     1801 tgttgttaac gcgctttcta cacagcttga tgtcaaagta tacaaagaag gaaatgtgta
     1861 ctatcaagaa tatcgtcgtg gagctgtagt ggacgactta aagattatcg aacaaacgga
     1921 tcgccatgga acaacggttc actttacgcc agaccctgaa atttttacag agacaactga
     1981 gtttgatttt tcaaaattgg ctacccggat ccgtgaactt gctttcttga accgcgggat
     2041 gcgtatatcg attgaagaca aacgagaaga agaaccagtc atcaatgaat accattatga
     2101 cggcgggatt aaaagttatg tcgaatactt gaatgcaaac aaaaccgtga tttttccaga
     2161 acctgtatat ttggaaggtg aacagcaaga tatcgcagtt gaagtctcta tgcaatatac
     2221 agacggctac cattccaata tcatgagttt tgccaacaat atccatacgt atgaaggcgg
     2281 aacacatgaa tcaggattca agactgcttt aactcgtgtg ataaatgatt atgcccgaaa
     2341 acaaaagctg atgaaagaaa atgacgacaa cttgaccggt gaagacgtgc gagaaggatt
     2401 gacagctgtt atttctatca agcatcctga ccctcaattc gaaggacaaa caaaaacaaa
     2461 attgggcaat tccgaagtac ggacagtcac tgatcggtta tttagcgaac atttcatgaa
     2521 attcttgctg gaaaacccaa gtgtgggccg tcaaattgtt gaaaaaggat tactagcttc
     2581 cagagctcgt ctagcagcga aacgtgcgcg tgaagtaacg agacgtaaag gtgctttaga
     2641 gattagtaat ctgccaggaa aattagcgga ctgctctagc aacgatcctg aaaaatgcga
     2701 attgttcatc gtcgaaggag attcggccgg cggatcagct aagcaaggtc gtaatcgaga
     2761 gtttcaagcc atcttgccta ttcgtgggaa aatcttgaat gttgaaaaag caagcatgga
     2821 caagatcttg gcaaatgaag agatccggtc tttatttact gctatgggta cagggtttgg
     2881 agaagatttt gatgtatcga aagctcgcta ccacaaatta gttatcatga ctgatgccga
     2941 tgtcgacggc gcgcatatcc gtacattgct gctgactctt ttttatcgtt acatgcgtcc
     3001 tattgttgaa gctggatacg tctatattgc tcagccacct ttatacgggg taaaacaagg
     3061 gaagaatatt acgtatgtac aaccaggaaa aaatgcagaa gaagagttga aacaagtcgt
     3121 tgcttcactt cctgcctcac caaaaccaag cgttcagcgt tataaaggac ttggagaaat
     3181 ggatgatcat caactatggg aaacaactat ggacccaagc aatcggatga tggcccgcgt
     3241 cagtgtggac gatgctattg cagcagatca gatatttgaa atgttgatgg gggatcgagt
     3301 agaaccccgt cgtgcattca tcgaggaaaa tgcacactat gtgaagaact tagatattta
     3361 atcatgaagg aggaaaaaga atgagtgaag aaatcagaga aaacatccat gatgtcaatc
     3421 taaccagcga aatgaaagac tccttcatcg attatgcgat gagtgttatt gtcgcacgtg
     3481 cacttcctga tgtcagagat ggtctgaaac ccgttcatcg ccgtatttta tacggcatga
     3541 acgaattagg tgtaacaccg gataaagccc ataaaaaatc tgccagaatc gtcggagatg
     3601 tcatgggtaa ataccatcct catggagact atgcgatcta tgagtcaatg gtacggatgg
     3661 cacagccatt cagctaccgt tatatgctag tggatggtca tggaaacttt ggttctgttg
     3721 acggtgacgg tgccgccgct atgcgttata ccgaagcacg gatgagcaaa atcgctacag
     3781 aaatgctgcg tgatatcaat aagaacactg ttgatttcca aagcaactat gatgatacag
     3841 aaaaagaacc agttgtacta cctgctcgct tcccgaatct tttagtgaac ggaacaacgg
     3901 ggatcgccgt aggtatggcc acgaatatcc cacctcataa cttgtctgag gtagtagcgg
     3961 ctatcgattt gctgatggat aatcctgatg tgacgaccaa tgaattgatg gaagtattac
     4021 ctggaccaga ttttccaaca ggtggtttgg ttatgggaaa atcaggaatc cgaagagcgt
     4081 atgaaacagg taagggttct attaccgtac gtgcaaaagt tgagattact gagatgccaa
     4141 atggtaaaga acggatcatc gtgacagaat tgccttatat ggtcaataaa gcaaaattga
     4201 tcgaacggat ttctgaactg cacagagaca agcgtatcga agggatcaca gatttgcggg
     4261 acgaatcttc ccgtgaaggt atgcggatcg tcattgacat ccgtcgtgac gccagcgcat
     4321 ctgttatctt gaataactta tataaattga catccttgca aaattcattt ggattcaata
     4381 tgttggcgat cgaaaaaggt gtgccgaaag ttttgagctt gaaacaaatt cttgaaaact
     4441 atatcgaaca tcagcgcgaa gtcatcacaa gacggacagt ctttgaaaaa gaaaaagcag
     4501 aagcaagggc acacatttta gaaggtttgc gtattgcttt ggatcatatc gatgaaatca
     4561 ttgcgattat ccgcgggtct aaatcagatg acgaagcaaa agcgacgatg atcgaacggt
     4621 tcgatttatc cgatcgccag tctcaagcta ttttagatat gcgtttacgc cgattgaccg
     4681 gtttggaacg ggaaaaaatc gaaaatgaat accaagaact cttaaaactg attgaagatt
     4741 taactgacat tctggcaaga ccagaacgtg ttacagaaat catcaagaat gaattagcag
     4801 acttgaatca acgatttggt gataaacgac gcacagaatt actagtcggt gaggtcttaa
     4861 gtctggaaga tgaagattta atcgaagaag aagaaatcgt tattacattg acaaacaatg
     4921 gttacatcaa acgtttggca aataatgaat tcagagctca acgaaggggt ggacgtggtg
     4981 tccaaggaat gggtatccac aatgacgact tcgtcaaaaa tctggtttct tgttctaccc
     5041 atgatacatt gttattcttt acgaataacg gaaaagtgta tcgggcaaaa ggatacgaaa
     5101 ttccagaata cgggcgcaca gccaaaggaa taccaattat caatttacta ggtatcgatt
     5161 cgagtgaaag catccaggca atcattgcgg tgacgggaga agcagaagaa ggccactatc
     5221 tcttctttac gacgcgtcaa ggtacagtaa aacgtacttc tgtcactgct tttgctaaca
     5281 tccgaagtaa tggtctgatc gcaattggtt tgaaagaaga cgatgagctc gtcaatgtgc
     5341 tactgacaga tggtcaatct aatatcatta ttggaacaca taacggctac tctgtcactt
     5401 tcaaagaaga agcagtccgc gatatgggac gtacagcttc cggggttaga ggaatacgcc
     5461 tgcgtgagga agattatgtc gttggggcag ccttaatgaa ggaagatcaa gaagtattag
     5521 tgatcacaga aaaaggttac ggaaaacgga caaaagtatc ggaatatcca gtgaaaggcc
     5581 gcggaggtaa agggatcaaa acggcgaaca tcactgaaaa aaatggtcca ctagctggat
     5641 tgaccactgt ttctggagat gaagacatca tgctgattac agataaaggt gtcattatcc
     5701 gtttcaacgt atcgactgtt tctcaaactg gacgttcaac attaggcgta cgtttgatga
     5761 agatggaagc tgatacgaaa gttgtcacga tggcagcagt cgaaccagaa gctgcagaac
     5821 aaacagttga agaacaagga tcagaagaat agccatcaag actaattgtg taagctagca
     5881 agaatgaccg attaaaaaat ctggttattc ctgctggctt ttttgattag ttgaatttct
     5941 cttaaaatat tcgaaactgt tcattctgta ttgcttttga ccaaaataga gtgtataatc
     6001 agagcttgtg agtagtctaa ttaattagat aactctcctt gctcttagga acctaagagc
     6061 cgaaagtcca cagggaggtg aatatcaatg gaaaacacga aatatgaaat catgtacatt
     6121 attcgtccaa acattgatga agaagcaaaa actgctttaa tcgaacgttt cgatgcaatc
     6181 ttgaaagata acggagcaga agttatcgaa tcaaaagatt gggaaaaacg ccgtttcgct
     6241 tatgaaatga acggttaccg tgaaggtatc tatcacatcg ttaaagtatc ttctccatca
     6301 tcagcaaatg ctgtcaatga atttgaccgt cttgctaaaa tcaacgacga tatcattcgt
     6361 cacatgattg ttaaagaaga agcttaattg ttgtttcatg tgaaacaata gaaaacgatg
     6421 agaggagatg actattttga ttaataatgt tgtattagtt ggacgtctga cgaaagatcc
     6481 agatttgcgt tacacagcaa gtggtacggc agtcgcaaca tttactttgg ctgtgaatcg
     6541 taattttaca aaccaaagcg gcaatcgcga ggctgatttt atcaactgtg tgatttggcg
     6601 caaatcagca gaaactttag caaactatgc tcgtaaagga acacttttag gtgttactgg
     6661 acgtatccaa actcgttctt atgataatca gcaaggacaa cgtgtatacg tgacagaagt
     6721 agtagctgac aacttccaat tgctggaaag tcgttctgca tctgaaaatc gccaacaaag
     6781 cggcggattc caaagttctg gtcaatcagc tggcggattt ggcggaaaca ataactctaa
     6841 tcaaacatct caatcatcca acggtatgcc tgatttcgac cgtgatactt ctgacccatt
     6901 cggctcaagt tcaacgatcg acatttcaga cgatgattta ccattctaaa cgaatttgat
     6961 aggagggaaa tagaatggca caacaaagaa gaggcggacg taaacgtcgt aaagtagact
     7021 acattgctgc taaccatatt gaatatatcg attataaaga tatcgaatta ctaaaaagat
     7081 ttatttcaga acgcggtaaa atcttgccac gtcgtgtaac tggtacaggc gctaaaaacc
     7141 aacgtaaaat tactgttgca atcaaacgcg ctcgtatcat gggcttgcta cctttcgtta
     7201 gcgaagacta ataaatccta ggaattgaat gactgatcct tttgatcagc tattcaattc
     7261 ctttttttct gtaaaaataa cctctattcg tttagtcgct cgaatgacga aagttatggt
     7321 aaaatcaaat tagtcatata tgggaggaaa attatggaaa ataaaaacaa tcatttatcc
     7381 ccgaaacctc tcttccttat cctgtttata ttaatcgaag tattggtagt cttaatttta
     7441 ccatttcgtt ttgttagcgc ttcggtcatt ctattgatga atttatgggg tgttcgctat
     7501 tggttgaaaa tgaaacgttg gcttatttta aataaacgtg aaaaaatcca gcaggcttcg
     7561 actgtagctg aaaccaatct agcttatgtc tcagaagtca tgcctgtagg agtgattgct
     7621 tatcagccat caagccaaaa aatcgaatgg ctcaatccat tagcattgaa aatcaaagat
     7681 caaagtggat taacagagga agaattgcta gatacttttt ttactgcaaa agaaaaaaag
     7741 aaaaaccaag tctatctagc agataaaaca tttcagtttg atatggatac agtaaagggt
     7801 gttgtttatt ttaatgatat tacccaaaac cagttgttaa aactccatca gacgaacagt
     7861 cagccggtga ttggtatttt atctattgat aactatgcag atgccattga taaattggat
     7921 gacaaagaga tttcttattt gaactctttc gtcactacac tagtttctga ctggatgaat
     7981 gaataccatg tctttttcaa aagaatcaac gcagaacggt ttttcttcgt agcacagatg
     8041 atcgatctga aaaagatgat ggataaaaaa tttgatttgc tggatcgctt tcgagcagaa
     8101 gctgaaaaac agagctcgcc tatcaccctt agtatgggga ttgcttatgg ctcaagaacc
     8161 ctcgatgaaa tcggtggaga agctcagaat aatttagatg ttgctttagt ccgtggaggc
     8221 gaccaagttg tcgtgaaaga ggtaaaagaa ggagcaaaac ctgtttatta tggggggaaa
     8281 tctgctaaga atgtcaaacg cacgcgtgtt cgttctcgag cgatgagtac cgctttgcgt
     8341 cggatttttg atgaaacagg cgatgtgttt atcatggggc acaagtttcc ggatatggat
     8401 gctttaggct ctgcttttgg tgtcagttgt ctggcacgct tcaaccagaa aaaagcttac
     8461 atcgttatca acgagaacga gatcattcca gatgtagaac ggtgtcttga agaaatccat
     8521 aagcatcctg aattagaaat gcaactaatc tctccagaac gtgccataga gctgaaaaaa
     8581 gaagaggacc tcctcgtgat ggtggattac cataaacctt ctctatcgat ctctcagggc
     8641 ctatatgagc aatttgataa gattgtgatc attgatcacc atcgtcgtgg ggaagagttt
     8701 ccaagtaaac ctctgctgac ttatatcgaa tcttcagctt cttcagcttc agagctagtt
     8761 tctgagctga tccaatacca aagcagcaaa acaaatcgct tagatcgttt tgaggccaca
     8821 ttattgcttg cagggatggt tgtagataca aatagtttca atgtccgtac aactgcacga
     8881 acttttgacg ttgccagcta tttaaggtct tgtggagcag acgcatcctt agtacaatat
     8941 ttattgagct cggatttagc gacatacttg gagatcagca atttgattgc tggaagcgac
     9001 tatgtgactt ctgatattgt tgtggcggca gggacagacg aaaaagagta taatagtgtt
     9061 atagcagcaa aaacagcaga tacattgctt tcgatgatca atatcaatgc agctttcgtc
     9121 attacaaagc ggacagacgg cttgattggg atcagtgcca gaagtaccgg cacgatcaac
     9181 gtccaaatca tcatggaatc cttaggtggc ggaggacact tcacaaacgc agctactcaa
     9241 ctggaaaata cgacagttga ggaaacaaaa cagaagctat ttgctgcaat caaagaaaat
     9301 atgaatcaaa tatatgataa ggagtaatcc aaatgaaagt aattttttta caagatgtaa
     9361 aaggaaaagg taaaaaaggg gacatcaaag aagttccagc aggttatgca caaaattatc
     9421 taatcaaaaa taacttggca aaagaagcaa cgaaaagcag tatcagtcag ttagcaggac
     9481 aaaagaaagc acaagaaaaa cacgaagctg aaatcttgga agaagctaaa aagttaaaag
     9541 aattcttgga aaaagaagat actgtagtcg aattgaaggc gaaagcaggg gaagacagcc
     9601 gtttgtttgg ttcgatccct tcaaaacaaa ttgctgaagg gttaaacaaa caatacaaaa
     9661 tcaaattaga taaacgaaaa atcgaattag aaaatccgat ccgttcacta ggctacacaa
     9721 acgtacctgt taagcttcat catgaggtga cggctacgat caaagttcat gtcgtacaag
     9781 aataacaaat cttgatagcg atgttcagct ggtaagtata accagaagga aaaaagacaa
     9841 accgaaattt atctgagaaa acagctgcag caatgaaagc aaaagctgca aatcgatttc
     9901 agattgattt cggtttcttt tttgggaaca gcaaaatagc ataaagaaaa cgagtataat
     9961 aaaagagaac tattttttta ttacactttc ggtacgaatg atgacttttc tctcattttc
    10021 ttgtaaaatg aagcaattga agacaaggag cgatacgtat gaatgaagtg tggcaggatc
    10081 gaatcccacc gcaaaatatt gaagctgaac aagcggtttt aggctctgtt tttcttgacg
    10141 cagaaacgat cattgatgcc atggagtaca tagagccaaa agacttttat cgtcgtggcc
    10201 atcaaattat ttttgagaca atgatcgagt taaatgatcg taatgaagcg attgacgtgg
    10261 ttactgtcaa agaccggctg gaacaagcca atttgttaga agatgccggt ggtttaagtt
    10321 atctctcaga cttggcatta gcagttccga cagcagcaaa tatcgtctac tatgcgaaaa
    10381 tcgttgaaga aaaatcattg ctgcgtacat tgatccagac agcaacagga atcgttacga
    10441 aaggattcga gcaaggagaa gaagttcaaa caattttaga tgatgccgaa cgcagtatct
    10501 tggaggtttc ggaaaaaaga aatcgaagcg gctttttatc tattgctgaa gtactgaact
    10561 ccacgattga aaatatcgat cgactagctc aaaacaatga agaaatcaca ggtctaccaa
    10621 ctggctatca aggtctggat aaaatgacag caggtttcca gcccgaagaa ctaatcattc
    10681 ttgctgcacg tccagcggta ggtaaaacag cgtttgcatt gaatattgct caaaatgtag
    10741 gaacaaaaac agatcgttct gttgcgatat ttagtttgga aatgggggca gaatctcttg
    10801 taaatcggat gctttgtgca gaaggttcga ttgaggccag ccatttacgt acaggtcagc
    10861 tttcggaaga agaatggtcg aatctgatcg ttgcgatggg gagtctttct cgagcaaata
    10921 tttttatcga tgatacacca gggatcaaaa tctctgaaat ccgtgcgaaa tgtcggaaac
    10981 tagcgcaaga aaaaggcaat ctaggcttga tattgatcga ttatttgcag ttgattgaag
    11041 gtaacggtaa agagaaccgt caacaagaag tatcagatat ctctcgccaa ctgaaaaaac
    11101 tggcgaaaga gctgaaagta cctgtcattg ctctttcaca gttatctcgt ggagtagaac
    11161 agcgacaaga caaacgacca gtgctaagtg atattcgtga atccggatca attgaacaag
    11221 atgcggacat cgtagccttc ttgtatcgag atgattatta tgaaagagaa ggcggagatg
    11281 acgatgatgg aaacgagcca aaaaatgaca acgtcatcga agtcatcatc gagaagaacc
    11341 gaagcggtgc acgaggaaca gttgaactac tgtttatcaa agaatacaat aagttttcgt
    11401 cgctttcgcc aagagaagaa ttttaaagaa gaggttgtga gccttgagca atcccgagag
    11461 ctctcgtaag aagatgcaag gagtaaccgc aggaacgact agagtactta atttaaacag
    11521 atctccaaag ggattgtgac aaaagtcttg tcacagtccc tttttccgaa taaacggtgt
    11581 tcaatcagct gacccttggt attagctcag caaacgtcaa aatatgagga actattttga
    11641 cgtttgtttc tctaatactc aggtcaaaac gctgatctta cgacctcttt tccttaaacg
    11701 gtgttctcct gccaactcct cggtcacaag tcacaatatc atcaaacatg agaagcagtt
    11761 ttttgaaatt gctccttgtg actcagagtt aaacggcagg ctcacaacct ctttcttaaa
    11821 cggtgttcaa aagcttgcat ctgcggtaat acgcccaatc aaacgaaaaa tatgagaagc
    11881 tatttttgtt tggctgttct tattacttga tgcaggacag ctttttcaca acctctgatt
    11941 cacggtgttc caaaagtagc ttcttcgaaa aataagtcga aatttcacaa aaatttgaga
    12001 agcaattttc gtacattcct tcttatttct tgaagctgac cacttctgtt acaacctctt
    12061 ttttaataac cattctaaaa agataaagtt cgttttttaa gcgtttttat aaaataaaaa
    12121 taaaaatcat tcgattttat ttgaaatatg tatctattat tgatacaatg gttcaggatt
    12181 aaataatata gaacgaggtg ttcacatgtc atctgtagta gtagtaggta cgcaatgggg
    12241 cgatgaaggg aaaggaaaaa tcacagactt tctcagtgaa aatgctgaag tgattgcacg
    12301 gtaccaaggt ggcgacaatg cagggcacac aattaagttt aacggtgtga cttataaact
    12361 gcatttgatt ccttctggga ttttttataa agacaagatc agtgtgatcg gaaacggcgt
    12421 tgtagtcaat cctaaatcat tggttaaaga attagcttat ttacatgaaa aaggtgtaga
    12481 aacaagtaat ttgcgtattt ctgaccgcgc gcacgtaatc ttgccttatc atatcaaatt
    12541 ggaccaatta caagaagatg ctaagggcga aaataagatt ggtacaacga ttaaaggaat
    12601 cggacctgcc tatatggata aagcagctcg tgtaggtatc cgtattgcag acttattaga
    12661 taaagaaatt tttgaagaac gtcttcgttt gaatctagat gacaaaaacc gtcaatttgt
    12721 aaaaatgttt gattcagaac cacttgattt tgacgatatt tttgaagaat attatgaata
    12781 cggacaacaa atcaaacaat acgtcacaga tacttccgtt atcttgaatg atgctttgga
    12841 tgcaggcaaa cgcgtgttgt ttgaaggcgc acaaggtgta atgctggata tcgaccaggg
    12901 aacttatcct tttgttactt cttctaaccc agtagccggt ggggtaacaa tcggaagcgg
    12961 tgtagggcct tcaaaaatca ataaagttgt cggcgtctgc aaagcctata cgtcacgtgt
    13021 cggtgatgga ccatttccaa ctgaattatt tgatgaaaca ggagaacaaa tccgcgaagt
    13081 aggacgagaa tacggtacga caactggtcg tccgcgtcgt gtaggctggt ttgattctgt
    13141 agttatgcgc cattctaaac gagtttcagg tattaccaac ctatctttaa actcgatcga
    13201 tgtattaagt ggattggaaa cagtaaaaat ttgtacggct tatgaattag atggcgaact
    13261 aatctaccac tatccagcaa gcttgaaaga gttgaaccgc tgcaaaccaa tctatgaaga
    13321 attgccaggc tggtcagaag acattacagg atgcaaaaca cttgcagaac tgccagaaaa
    13381 cgcacgtaac tatgtacgtc gtatctcaga gcttgttggt gtacgtatct caacattttc
    13441 tgtcggacct gatagaacac aaacaaacat cttagaaagc gtctgggcgc aaatctaagg
    13501 ataaaaacga cgactgaatc aggacgttct taccagagcg agtcgaagtg tttatctata
    13561 cgataataat aacttgaaca ctgtttattc agaataaggg actgtgacaa aaaccgtgtc
    13621 acagtccctt tttccgaa
//
LOCUS       DARBDV010000078        13429 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000078 DARBDV010000000
VERSION     DARBDV010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.079"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..914)
                     /locus_tag="SCM56_002188"
     CDS             complement(<1..914)
                     /locus_tag="SCM56_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEG1110938.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="SCM56_002189"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="SCM56_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEG1110939.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2006..2176
                     /locus_tag="SCM56_002190"
     CDS             2006..2176
                     /locus_tag="SCM56_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110940.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEETALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            2391..3650
                     /gene="lysA"
                     /locus_tag="SCM56_002191"
     CDS             2391..3650
                     /gene="lysA"
                     /locus_tag="SCM56_002191"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEG1110941.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3637..4437
                     /gene="dapF"
                     /locus_tag="SCM56_002192"
     CDS             3637..4437
                     /gene="dapF"
                     /locus_tag="SCM56_002192"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEG1110942.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4440..5321
                     /locus_tag="SCM56_002193"
     CDS             4440..5321
                     /locus_tag="SCM56_002193"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEG1110943.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5318..6034
                     /locus_tag="SCM56_002194"
     CDS             5318..6034
                     /locus_tag="SCM56_002194"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEG1110944.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6040..6735
                     /gene="dapD"
                     /locus_tag="SCM56_002195"
     CDS             6040..6735
                     /gene="dapD"
                     /locus_tag="SCM56_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEG1110945.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6751..7752
                     /locus_tag="SCM56_002196"
     CDS             6751..7752
                     /locus_tag="SCM56_002196"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEG1110946.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7749..9110
                     /locus_tag="SCM56_002197"
     CDS             7749..9110
                     /locus_tag="SCM56_002197"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEG1110947.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9070..10221
                     /locus_tag="SCM56_002198"
     CDS             9070..10221
                     /locus_tag="SCM56_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEG1110948.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10196..11365
                     /locus_tag="SCM56_002199"
     CDS             10196..11365
                     /locus_tag="SCM56_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEG1110949.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11540..12919
                     /locus_tag="SCM56_002200"
     CDS             11540..12919
                     /locus_tag="SCM56_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEG1110950.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13200..13429
                     /locus_tag="SCM56_002201"
                     /pseudo
     CDS             13200..13429
                     /locus_tag="SCM56_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4482 a         2340 c         2926 g         3681 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaagaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtataaatag cttctaatag
     2221 ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat atttcgaaat
     2281 atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca aatctttatc
     2341 aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat
     2401 tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg
     2461 tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga
     2521 aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc
     2581 atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg
     2641 ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag
     2701 aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg
     2761 aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc
     2821 ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt
     2881 caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag
     2941 aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg
     3001 ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac
     3061 ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga
     3121 tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac
     3181 gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg
     3241 gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg
     3301 tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg
     3361 atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt
     3421 gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc
     3481 ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag
     3541 cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac
     3601 aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa atataatggc
     3661 tgcggaaatg attttatctt attagattac gcagaagaca cagattatag tcaattagcc
     3721 gcttctttat gcacaaaaga aaaatatgat acagacggat tgatcgccgt aaaaatcgat
     3781 ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc
     3841 attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac
     3901 gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc
     3961 agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat
     4021 ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt
     4081 ttaggaacga ttcatacagt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa
     4141 agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta
     4201 gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca
     4261 cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt
     4321 tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat
     4381 gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga
     4441 tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt
     4501 atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag
     4561 ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc
     4621 aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata
     4681 atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt
     4741 tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag
     4801 caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca
     4861 tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga
     4921 aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat
     4981 tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg
     5041 gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata
     5101 cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc
     5161 atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact
     5221 taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat
     5281 tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag
     5341 tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct
     5401 tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct
     5461 tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca
     5521 acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga
     5581 acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat
     5641 gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga
     5701 aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac
     5761 aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga
     5821 aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt
     5881 gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt
     5941 tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa
     6001 attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc
     6061 cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag
     6121 ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt
     6181 gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa
     6241 atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct
     6301 cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc
     6361 atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg
     6421 ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct
     6481 gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta
     6541 ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg
     6601 gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct
     6661 gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg
     6721 ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg
     6781 gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta
     6841 ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa
     6901 aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta
     6961 ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta
     7021 tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca
     7081 gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc
     7141 attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat
     7201 tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt
     7261 acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac ggtgattccg
     7321 gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag
     7381 acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca
     7441 atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa
     7501 gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca
     7561 cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc
     7621 agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc
     7681 aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag
     7741 gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg
     7801 aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg
     7861 ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt
     7921 tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg
     7981 atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag
     8041 cacgcataca gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag
     8101 gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa
     8161 aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa
     8221 tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc
     8281 ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg
     8341 cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg
     8401 ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga
     8461 gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag
     8521 aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa
     8581 tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt
     8641 ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag
     8701 caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca
     8761 atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag
     8821 aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct
     8881 ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat
     8941 tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga
     9001 tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg
     9061 tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt
     9121 catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcgg ggccgttatc
     9181 catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt
     9241 gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct
     9301 tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta
     9361 gggttgactg actttataac taaacacatt gaggatctta tttataatgt agtactgatc
     9421 tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg
     9481 gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga
     9541 gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt
     9601 aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct
     9661 gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat
     9721 ctaatgaaat ttggccaaat ccaaggtgga acgatccgca atgttttagc tggagaagtt
     9781 attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga
     9841 ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac
     9901 ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa
     9961 ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa
    10021 tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg
    10081 ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt
    10141 acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat
    10201 gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca
    10261 attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga
    10321 ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc
    10381 tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca
    10441 acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac
    10501 ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc
    10561 agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc
    10621 gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac gagcatttga
    10681 acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac
    10741 gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt
    10801 tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc
    10861 tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac
    10921 tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt
    10981 ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct
    11041 aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagacta
    11101 tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta
    11161 cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt
    11221 agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg
    11281 ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat
    11341 caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt
    11401 tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc
    11461 ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat
    11521 attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg
    11581 agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct
    11641 atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga
    11701 caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa
    11761 tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac
    11821 ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag
    11881 ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt
    11941 gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa
    12001 aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa
    12061 agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga
    12121 tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac
    12181 aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga
    12241 aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg
    12301 gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca
    12361 aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc
    12421 acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt
    12481 aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc
    12541 attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa
    12601 agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc
    12661 agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca
    12721 attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc
    12781 actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca
    12841 gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga
    12901 agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct
    12961 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg
    13021 acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac
    13081 acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg gaggtcggta
    13141 ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga ttatttggat
    13201 ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaaa
    13261 ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg
    13321 gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga ataaaagtaa
    13381 cggcatacac gcgtaaatca cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DARBDV010000079        13396 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000079 DARBDV010000000
VERSION     DARBDV010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.080"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(15..707)
                     /locus_tag="SCM56_002202"
     CDS             complement(15..707)
                     /locus_tag="SCM56_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEG1110951.1"
                     /translation="MQIELQHTHDKETFEKNYKVYFELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(1144..1713)
                     /locus_tag="SCM56_002203"
     CDS             complement(1144..1713)
                     /locus_tag="SCM56_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEG1110952.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1787..2110)
                     /locus_tag="SCM56_002204"
     CDS             complement(1787..2110)
                     /locus_tag="SCM56_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110953.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2264..3076)
                     /locus_tag="SCM56_002205"
     CDS             complement(2264..3076)
                     /locus_tag="SCM56_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110954.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3430..4278
                     /locus_tag="SCM56_002206"
     CDS             3430..4278
                     /locus_tag="SCM56_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEG1110955.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4423..5364
                     /locus_tag="SCM56_002207"
     CDS             4423..5364
                     /locus_tag="SCM56_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEG1110956.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5354..7525
                     /locus_tag="SCM56_002208"
                     /pseudo
     CDS             5354..7525
                     /locus_tag="SCM56_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7637..9808)
                     /gene="copB"
                     /locus_tag="SCM56_002209"
     CDS             complement(7637..9808)
                     /gene="copB"
                     /locus_tag="SCM56_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HEG1110957.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9828..12014)
                     /locus_tag="SCM56_002210"
     CDS             complement(9828..12014)
                     /locus_tag="SCM56_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEG1110958.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12014..12223)
                     /gene="copZ"
                     /locus_tag="SCM56_002211"
     CDS             complement(12014..12223)
                     /gene="copZ"
                     /locus_tag="SCM56_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HEG1110959.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12236..12676)
                     /locus_tag="SCM56_002212"
     CDS             complement(12236..12676)
                     /locus_tag="SCM56_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEG1110960.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12750..13289)
                     /locus_tag="SCM56_002213"
     CDS             complement(12750..13289)
                     /locus_tag="SCM56_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEG1110961.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3763 a         2807 c         2381 g         4445 t
ORIGIN      
        1 gaggggggga ttttttatcg ttgtggaata tttaaatacc aacgcttgtc atgaaaatct
       61 tgtgctccac ccttagtatt tccttctgga tcgtttgtcg cacgcatcat gacgtatact
      121 ttcttattag ggaaatcacg catattgaaa gatacatgat agccaacatt tcctaaagta
      181 ttataagctt gatttacgtc tggtctataa atcccatcag ctcttactct agctagttct
      241 tttccagtat tgtagtccat aatgaaaata tactcgtatt tataattagc aatgtgccat
      301 ccagctacat gcaagtttgc gttttcgatt tctccgaact gatcaatgtg ggcgtaattt
      361 gttccatctg tcagcgtaga atttgcagca cctgctcgag ttggatcaat tactggttta
      421 tcctccgaag tagttggatt atcatccgta aaaccatgag ccaaatcata ggctagtttt
      481 tctttactta cacccatttc agaaagataa ccgtaaggat ctgtatgatc gccccaaata
      541 ttttgtgtta cccataaatg cgatttgatt cctggttggt tataaggagt gtctaatgtt
      601 aatggaatac catatttcat tgctgaatct cttgccaatt caaagtatac tttatagttc
      661 ttttcaaaag tttctttatc atgtgtatgt tgtaactcga tttgcacagg acttttggct
      721 cacgaggttg tcctactcta catcttgctg atagatatgc tgaaaggaaa acaccaacat
      781 tatgttttgc tttttcagct agaacgagac aagcaggaat acctttagaa gatatcaaag
      841 gttttttagg tcataaagat gtttccacta cacaagtcta tgcccatatt tctcccgaag
      901 tgaaaaaaag atcaatgaat caactagaaa actatataga agagcaaata aaaaagcact
      961 caaattgagt gcttttttaa gctcactatc actaaaacta tcactaattt tatttataag
     1021 cgaacatgtt tctatcactg attttttaaa tctagcgaaa aacccttatc ttaaaggcgt
     1081 tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc ctagaagtgc gaacatggtt
     1141 ttcttatagc tcatgtagaa aaattttctt gatgatgatt ttatccatca attgagattg
     1201 actgttttgc tctcctatga tttcagctat gattgtccga ttttgcggtt ttcttcttaa
     1261 ctggtagttg attttcccag tttccatcaa ctcgctaagc atatccacga ctccgccaaa
     1321 tccggtcttt ttcgtaataa aacctagtgc taccattcgt tcatctagat tttttgctgt
     1381 aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg gccagctctt ccatcccttc
     1441 agcattggat acgacggaag ctgttttgtt aataggttct tcgtttattt cttttggttc
     1501 tttcctctcc actttcactg tttgacgaag gacatctagt tcttgcaaga gcgtttcgta
     1561 ttgttcttgg gtcagcatca cgccagctac tttttcacga ttaaatatat atacgccagt
     1621 tcccgcgtct cgcgcttgag caaatacatc catcggcgat cgtttcactt ctgtgatcga
     1681 agatgtggga acttctaatt tctttaattc caatggattt cctcctttac taatcgcttt
     1741 ttattatagc acagactgac ttgttttgat cttttttcca aaggttttat cggtttttgc
     1801 ctaaccagta attgatatcg gtaagaagtc tttcgatacc aaatgaagga tggtacagat
     1861 acgttttttg atcttcgtta tggtctagtg gaaaaaaatg atcgatgatc actagatctg
     1921 cataccgagg cggcttattt cgagtacata catgtataga gatccgtttt cgatgtctta
     1981 gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg gtgttcatag cgaaaaaaaa
     2041 agacgatttt cacttcagga gggtggacat ctaaataccc tcgttcctgg agtgttttca
     2101 gtaaaagcat cccccactct actgcggggt gtgcaagtgc ttgttggatc gctagccgaa
     2161 attgatatag tttatctttg gaattgggaa atttttctaa tgtttcctga taataacgaa
     2221 cttcttctat cagccggtca tggataccaa ttacgtaacg aagtcattct tggtaaagta
     2281 cttttgtcag ctttgatttg ctttcgctct caaattcgct attcagccaa aacagcgaat
     2341 caagcttttc agtcacaatc gtcaatttcc gatagtcttg tgtctcttct atccgtttcc
     2401 accgcttttc agtaatctgg ttacagctca agcgcaaaat tgctagataa tttttttctt
     2461 ttattggcaa aatctgctcc tctctaaata gcaattcagg aaacaattca aagaatagtg
     2521 ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt tctttgcttc aataattgga
     2581 tgaacatctc agaaaaggac tgctctccat catctgcaaa aaaatgcgga aaatctttca
     2641 aaaagcaaaa cagcagatat cttacttttc tttctgaacc aaagatctgt gcttttttat
     2701 aagaaaaaga aaggtggtac tcgccaaacc actttctcaa tcgctcttgc gtagaacgat
     2761 cgtacttctg ctcgtaaggt tcattcaata gcagattttt cagtaaacaa aacgggggtg
     2821 attggatcag aaaaacaaac aagagataag gttcgctata tccttgatca taaactaact
     2881 gaacggtttt cttatgaaga atcagctgaa tgggacagtc gctttccgcc aaacgtttca
     2941 gatgccgatc cactgtttta acatcgattt tcagttcttc agctaaatca gcttttcgcc
     3001 agatcaaatc tttcattaga ttgattagtg aaaataaatt ctgtgcattt tttgataata
     3061 gtttttgcca tctcatcgat catttctcac ctgatttcta aacatattgt ttgatcaaaa
     3121 tgggttcact cctaaaaaga taaaaaactc cctttctatt tatttaaata cgcaaatctt
     3181 cctcattatt ttttatttta ctcaatttaa tcgtattctc atttcaaaac gaatgtactt
     3241 taaaaaaaca taataagatc actttgcttg tatgatgtat tcccttccat tacaatgaaa
     3301 taaatagcga ttttatgaga aggtgatgtg aaaatggaag aaaaaataga agaatccaaa
     3361 caaaaaatcg atcagttgat ataagaatta gaaaaagcgg tcatcagcaa acgttcaact
     3421 atccaattga tgatcgtggc cctattagca ggtggtcatg tcttattcga agatattccc
     3481 ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag ctgtacaggg aaccttcagc
     3541 agagtccaat gtactcctga tctgctgcct agtgatattc ttggtttttc cgtatacaat
     3601 tcacaatcaa aagagtttga atttcgtccg ggaccgatct ttaccacgat cttgttagca
     3661 gatgagatca atcgtacgac tcctcgtact cagtcagcac ttttagaagc aatggccgag
     3721 aatcatgcaa cgatcgacaa caatacatac cctctagaca atcatttttt tgtcttagct
     3781 acgcaaaatc caatcgagta cgaaggaaca tatcctttgc cggaagcaca gctcgatcgt
     3841 tttttattcc gactacaaat cggttatcct tcttttgatg acgaactttt gctacttttg
     3901 gataaattcg aaaaagtgtt ggataactta aatgaagtat tgaacagtta tgaaatagaa
     3961 gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg aagtggcatc ttatgccttg
     4021 cagttagtgt cagcaacaag aagtcatgct gccatccagc ttggcatcag tccacgagga
     4081 agcctttcct ttattcaggc agcaaaagct tatgctttaa tccatgggag aaattacgtc
     4141 acgcccaaag atttgcagga tttagttcct tacgtgtttt cccatcgcct gattttttac
     4201 gatcgttcat tgaaggaaga cgaaaagaaa caaatcattc atactcttgt tgcacaaatt
     4261 cctattcctg tgaggtagat tgaggtagac aatggaaaaa atcaaagatt atttcattaa
     4321 ttttgtgtta tttctggtat atttcatcat tgtgcttttt gctcttgtat tcaacaattc
     4381 tttaggctgg ttcttgttgg ccttttttac tctttatgta tcatggagct tgatttcatt
     4441 aagcagcttt ctaaagaaac tttcggttac ttgtacattg cagccactcg tttatcgcaa
     4501 aaaaagtatg caggttattt tttccatagc aaaaaagaga actttttctt tacctctccc
     4561 tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa aacaagagat tttgattctt
     4621 acaaataaac cgcaagaagt ccaattgctt tggcagccaa aagaaagagg atgccatgaa
     4681 acattaactg tcatttttac agcctatgat agtctaggac ttttcagaaa aagtgcaaaa
     4741 agagagatct cttttcctgt gactgttctg cctgcttttt gtaagattca agcagaaaaa
     4801 ttacagagag ttctggaaaa aaaacagcag ctgaacgcgt accaaagagg tcttgatttt
     4861 cgggaacatc gcgcttatcg tccaggtgat tctttcaacc gaatcgattg gaaattgtct
     4921 gctcatactc aagagtggtt ataccgtgaa tacgattatc aagaagaaga atgttctcct
     4981 cttttttgct tttggggttt gcccagtcca aaatttgaac ctctgctaga cttgtatttt
     5041 tctttatggc atttgagaaa agaaaagcag ccggaacttc tgatattagg caaccgcgcc
     5101 tttcatggca aagaccccgg tcatacctat tttgcttctt tggaagctgg cgatgagaaa
     5161 gcttttttcg cacacttgaa aaaatatgca aaaaaacaaa tcgtcctctt tgttcctgag
     5221 aattctcctg aagtctcaga agctattgaa acattgtcaa aagatcgaac ggtttatctt
     5281 gtttatttcg tagaaaaaca actcatggtc caagaaaaag acaagatatg ttcattgcca
     5341 ggaggtgaat ggattgacga ttaaacggga aaaatggctg cgtggcgttt actcctttgt
     5401 gatactaagt gtaggtgctc cgatatttgt agtagctaat caattgcaaa acagctacct
     5461 tcttatccta actattggtg tcctatgcct tattgtctgc tgtattccta aaaaatgggt
     5521 acgtttgctg ctttctttgc cagtaatgat aggttgtttg tttttatatt ttccttcgaa
     5581 gacattttcc atattatgga tcttacagtt tttttcgtca gtcacagagg aattcttcca
     5641 tctatcccaa ggtaaacttt attatttgcc agaaaagaca gctttttttc tcataatgtt
     5701 gctgatctat ttatttatta cattgaccgt ggattacgac tattggtacg caccctttct
     5761 ttctttgatg gcttacttca tgatgcttac cgtttttcgg aaaaaagatt tactgtacga
     5821 aatgattgcg gtagtaactg ttttgttcgt gtatctggca gcaaggcaat ttttctccat
     5881 ccggttgttg tcgggaaaca ctcgattcca gtcattgact acagttctgc ttatttgttt
     5941 cttaactttt agcgctatct tgcctttata tcttccgaaa gtacaccaat ctctcgagga
     6001 aacgagtgaa ccgatccgag aatatcttaa tgatgaaggg ctttatgaca cactccatga
     6061 gtatcaatta accggaagtg caagaacagg cttcagtgag aatgatgaac agcttggtgg
     6121 acctttatat gataatggcg aagttgtttt tacagcgaac caaaaaggaa atggctattg
     6181 gaagatcgaa aataaaaatc attacaccgg ttctggatgg atagaagatc aggacgcacc
     6241 catccaacca atccaagaat tccctgttga catcacgagc taccaaaccg ttatagaaga
     6301 caaagaaaac attacattga ccctaaaaaa acaagttttc ttaccttatc cttatggaaa
     6361 tactaccttg actgattttt cattagctag tggacaaacg ctttactatt tgcctgaaag
     6421 ccagcgattc attcctgtcc cgaatgaatc gacacgttct gcaactttac aaacagaaag
     6481 gttagttttt acgagagaag aattaaaaag cagtcagtta aaagctgatg atttagaaaa
     6541 tcagcagaca aatcttcaat tgccaaaaag tttgcctaaa cgtattgagg acttggcggt
     6601 cgaattgacc aaaaatgaag caactttaat tcgtaaagtc gaagctgtct aacagtattt
     6661 aaaaacaagc ggaaatttcc gctattccaa aacggatacc gggcatactc caaaagaaca
     6721 cgattatgtg gattatttcc tctttgattc aaaagtcggc tattgtgaca acttttcaac
     6781 ttctatggtc gtccttcttc gaacacttgg tatcccagct cgctggacaa aaggttttac
     6841 aaatgggctc agaacaggca cagataatca agggaatgag gaatacagca ttctcaatag
     6901 tcatgctcat tcttggccag aggtatactt tcctggattt ggctggcttc catttgaacc
     6961 aaccccttca tttgccaatc caatgggttc acaaccagtt gctgaaacaa ccgaaaccgt
     7021 ccagcctgct atcgaacaac caaatgaaag cgaaaatatg gcagaaacta tttccagtga
     7081 gagatctgag gtcattccat cagaagaaac aacactcaca ccagcttata aaatcgtctt
     7141 attcagcctt ggagtgtgtt tatccgtctt gttgttcttt ctattgttat tcagaaaatc
     7201 tctgcgctgg acaagttatc ttatctatct tcattttttt gcttctacta atacgattgc
     7261 atatcaaaaa cttcttaacc ctttggaaac attggtaccg cgcaaacccg cagaatcttt
     7321 gcaggaatat agcctgcgaa tagaacaaga attcaagcta gatctgcgtt tcattcgatt
     7381 aactactcaa tatgaagcag aaatatatgg acgacaagca gatccggtaa aattcgataa
     7441 aaaaacctat ttagaaataa cgcatataat aagaaaaatg gtatacaaaa aaattctgac
     7501 aaaagggagc cgcttcaaaa aataagataa aatttctgga gaatctactt ctggaatgcc
     7561 gagaaccagc tgattgagta tcgattactc gaaaaaaagg ttgtgacacg ttttttgtca
     7621 caaccttttt tcattattat ctgattttca atgtcaaagc atttagagct acaactaccg
     7681 tacttaacga catcaagaca gcaccaactg ctggacttaa tatgatacca attggcgcaa
     7741 gaattccggc tgctagcgga attgcaagaa tattgtaacc tgccccccac cagaggttct
     7801 ggatcatttt ctttcgcgtt tgcttggcta gatcaagaaa atgcaaaata tcttttggat
     7861 cgctgtttgt caatacgaca tccgcagaat cgattgcgat atctgtccct gcaccgattg
     7921 ccatcccgat tgaagcacgg gctaaacttg gcgcatcatt gatcccatca cccaccatga
     7981 ttactttttt cccttggtca aggtatttct ggacaacagc ttctttatca tctggtaata
     8041 aaccgccata atattcttcg atccctaaat aatctgctac tgcttgggca gcgtttttat
     8101 tgtcaccagt aagcatgaca ggagtgattt ttctttcttt caatgttcgg ataaattctt
     8161 ttgcttctgt ttttactacg tcacctaaag caacgattgc agctagatga ttttctaaaa
     8221 tcaggaagct gatggtattt ccttgttctt ggtaaggttt caataatgct tgttctacgt
     8281 ttaatccgag acgctcaact tctttttcat taactatttt cacttgttgt tttttgactg
     8341 ttgcctctag cccgactcct gaaagatttt ttagattttc agcttgataa ggcttgattt
     8401 catgttcttt caaataattc ataatgccaa cagccaatgg atgattggca ttcgcttcca
     8461 aggctcctag atattgaagg atttcatttt tattgaacgc atcatcaagt acttcgacac
     8521 ccgttacagt aaattttcct tctgttagtg ttcctgtttt gtccagcatg atcacatcta
     8581 gatcattggc ttgctccagt gcattgcggt ttttcaaaag taatccgttt ttagctgcta
     8641 ttgatgtgga acgagcaaca actaatggga tagctaaacc tagagcatga ggacaggcga
     8701 tgataaagac tgttaccata cgttctaatg cattaggaag atctgtcaag aacaaccagg
     8761 cgatgaatgc caggataccc acaattaatg cgacataaaa cagccatttt gccactttgt
     8821 cagataatga ctctagcttg gatttttctc cttgtgcttt tcgcaccatt tccataacct
     8881 tcgctagata accattttca ccagttccag ttacttcgat ttcgatcgtt ccatccccat
     8941 tgatagaacc gccaatcact ttgtcattgg cttgtttttt gattcctctt gattcgccag
     9001 tcacagctga ttcgtcaaca attgtttcac ctttcaagat tacaccatct gttggcattt
     9061 tatcaccaga ccggacgatc aaacgatcac cttcgtttac ttctttcaat gaaactgtct
     9121 cttcttttcc atctttcgtc aagcgtttca ctgattctgg cagtaattct gctagttttt
     9181 gtaaagcatt gctggcattc gacacagcat tcatttcaat ccagtgacct agtagcataa
     9241 taacgatcaa agtagccaat tcccagaaga aatccatgac gtgtgtatgt ggattgagca
     9301 gatttgcgat aaacgaatag acactataaa tatatgcaac tgtaatcccc atcgcgatca
     9361 gtgtcatcat agcaggactt ttttgtttca attccatctt agctccactt aaaaatggct
     9421 gaccgccata gataaatagt atagttgcta agatcaatac gagccaatca gaaccaggaa
     9481 acgttacttg aaatggaaat tccattccca tcattggtga aaaaagaatg attgggatag
     9541 ctaggataag cgatagccaa aatttttgtt tgaaattccc catgtgcatg gaatgatcca
     9601 tgccgccgtg ttcgtgatcc atgtgcatcg aatggtccat ttgtttgctg tttgctatat
     9661 ctccatggtt atgtcccatg tgcatggaat gatccatctg actatggtca tgtttcatct
     9721 ccatctgttt ttcatgagat tctgtacttt ctttgtttac tacttctttt ttatgctcat
     9781 gttttttctc catatcgttc ccattcattt ttactccatc ccttctctta ctttttcatg
     9841 tgtcgattta agcacaggga gttcaacaat acactgacgg aactaaacgc catcgctcct
     9901 cctgcaatga ttggattcaa aaagcctagt gcggcaaaag gaataccaat cgtattatag
     9961 acaaaagccc agaaaagatt ttgtttgatt tttctcaatg tcaacctaga caactcgatt
    10021 gtttgtgcaa tggatgccaa actgctattc atcagcgtca catcggctgt ttccatcgca
    10081 atgtccgttc cacttcccat tgccataccg acatcagcta atgctaaggc cggtgcatca
    10141 ttgattccat cgcctgccat accgactttt ttgccatctt tctgaagttt ttctacgtag
    10201 gctgctttat cctctggaag tacttcagca aagatatgct ttggatcaat accaacttgt
    10261 ttaccaatcg tttcggctgc cagtttgttg tctcctgtca gcataaaaac atctaatcct
    10321 ttattctgga gctgcttgat tgcttgtttg acttctagtt tgatttgatc agccacggca
    10381 atcaacccga tcacttgctt ttcatcagct aaaaacatga ctgtttttcc ctgctgttct
    10441 aattctaacg cgtgttcttg gtattcatca aatgataggt tcaattcaat taatcgttta
    10501 cgtgtacccg caaaatagcg tactccgtta atcgtaccgc tgattccagc acctgggtgg
    10561 gctgtaaaat caatcatatc gtatgttgct gcttcttgca gttttccgta ttccacgatt
    10621 gctttaccta gtggatgttc agaagcttgt tctaaagtat aaaaaataga taggacttct
    10681 ttagaaccga ctagatctgt aactttgggc tttccttctg taatcgttcc agttttatcc
    10741 aaaacaatgc tgtctaagtg agcagctgct tctaatgctt ctccgccttt aattaaaatt
    10801 ccatttcgtg caccaacacc cgttcctacc ataatagctg ttggagtcgc taaacctaat
    10861 gcacatggac acgcaatgac taatacagag acactatgga tcaatgcgag ttgccaatca
    10921 ccagtgatca aacctgtagc tatgagcgtg ataaacgcga tgactaatac gatcggaaca
    10981 aaaatccctg agatccgatc tgcgattttt tggattgggg ctttgctccc ttgagcatct
    11041 tccaccattt gaatgatttg cgctaatact gtctgcttgc cgacttgtgt aacttccatg
    11101 tgaagtagac cattcgtatt gattgtcccg ccaaagagcg tgtcatccgg attcttttct
    11161 acaggaagat tttcaccagt aagcatactt tcatctatcg cactgcttcc tgaaataatt
    11221 cgaccatctg ctggaatctg ctcaccagga cggatgacta gttgatcacc aacgactact
    11281 tcttcaatcg ctaacgtttc ttctttcccg tttcttatca cttgggcggt ttttgtttga
    11341 agagacatca attgtttgat tgcattacct gttttcgttt ttgctgtatg ttccaaatat
    11401 ttgccgagca aaatcagcgt gatgatcata ctgctgcttt caaaataaag atcttggggg
    11461 tgtccacgga aaaatccgtt gtaaatgctc aatgcaaagg cagccgatgt tccaattgct
    11521 accaatacat ccatatttgg tgctttcgtc ttgattgcat ggtacgcacc tttataaaaa
    11581 cgagcaccga cataaaattg gacaggagca gacagcacta actggacgat tggaaaatga
    11641 aaaaagtgaa caatcgccgc atggcttcct aacatcatag caatcattga caacatcaac
    11701 ggcaaagtca aaaccgtact caatataaga tcgaatagca tttttttcaa atacgcttgc
    11761 ttttcttctg caattttttg tttgtgggct tcatcgtaca aaattgcacc atagccgatg
    11821 ttttctacgc tttggattaa tttttcagtg gtcgtccctt catatttgac acttgctttt
    11881 tcagtggcca agttgactgt ggcattgacc acaccaggtt gtccgttcag ctctttttcg
    11941 atcctagcag aacaatttgc acaagtcata cctgtaatga cgaacgtatc cattttggtt
    12001 tctgctgttg ccatcaaatc acctctgctt tataacccaa tccattgatt gcttgacaaa
    12061 tcttttcgga ttgaactttt gtttcatcaa acttgaccgt accgtttgct tttttcaaat
    12121 ttactttcac tttttgcacg ccgtcaagtg cgcttactgt ttcttctact cttgctgcac
    12181 aatgattgca gctcatacct tcgattgaaa attgttgttt cattttttct tcctcctatt
    12241 gatgttgttt acattcacat tgacctggga tacagttaca ttcgatcgta tctactggtt
    12301 gcttttgttc cagttgtttc aaaatctcat tgatatcttc ttgagtcaaa gtggcttctt
    12361 ctactagatc cgcaatcgtc tctccaatcc tttttgcaca gatatgggaa aaaagatttt
    12421 ccgtcgcact gcgaactgtt tccatctccg agacagctgg ataataaatg aatttttttc
    12481 cttcttgttc tgtccagatc acttcttttt ttaccaaacg acccagcaaa gttttgatcg
    12541 ttgctggttt ccaatccatc gaatcagcta agatctgcgt gatttcttgg gctgtcgtct
    12601 gtccaagtgt ccaaataaca cgcatgatct cccactctga atcactgatc tttactggcg
    12661 ttttgacttt ttccatttat ttcacacctt tcgtttacaa atgtaatcat tatcttatga
    12721 atacagatta cacttgtaaa caaaaactgt caattatttt tgctctctat tttcaattta
    12781 gttaaaaatt cttgtttctc tcgtcttgtt ttaaggatga tcagtttatt tcttgcatta
    12841 gctacttgga tcagctgctg tattttcggt tcattgtttt cttcaaaatg ccagataaaa
    12901 gataaaaatt ctaagtattc cctgtttagt ttctccgaaa atgattcagg catatctggc
    12961 cgagattttt tgtttatctt tctttttaga gaacgccaaa ctactcggta gatggcgaga
    13021 taacgcggca ctttaagcca aatgatctta tctgcttctt taatcctttc ctctaatgta
    13081 gaagtataat ttccttcaac gatccatgac ggattagata ccataaacaa ttgctgttct
    13141 tgcaaaaacc acttttttgc ctgaatactg tagtctgttg tatgccataa aaggtctagc
    13201 ggcagtagcg gaaagtcggt gatttctttg atcttccttg cgagggtcga ttttcccgac
    13261 ccactttgtc caatcacgat gatcttcatt cttctccctc acttctcttt ggaatttggt
    13321 tcgatcatac cacttataac gccgcaaaaa aacaagggac tgtgacaaaa gtcgtgtcaa
    13381 agtcccttgt ttcgaa
//
LOCUS       DARBDV010000080        13180 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000080 DARBDV010000000
VERSION     DARBDV010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.081"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..1227)
                     /locus_tag="SCM56_002214"
     CDS             complement(<1..1227)
                     /locus_tag="SCM56_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEG1110962.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDIL"
     gene            complement(1224..3413)
                     /locus_tag="SCM56_002215"
     CDS             complement(1224..3413)
                     /locus_tag="SCM56_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1110963.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            complement(3547..4737)
                     /locus_tag="SCM56_002216"
     CDS             complement(3547..4737)
                     /locus_tag="SCM56_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HEG1110964.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            complement(5609..7240)
                     /locus_tag="SCM56_002217"
     CDS             complement(5609..7240)
                     /locus_tag="SCM56_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HEG1110965.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(7254..8927)
                     /locus_tag="SCM56_002218"
     CDS             complement(7254..8927)
                     /locus_tag="SCM56_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HEG1110966.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(8940..9257)
                     /locus_tag="SCM56_002219"
     CDS             complement(8940..9257)
                     /locus_tag="SCM56_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEG1110967.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(9282..10682)
                     /gene="lacG"
                     /locus_tag="SCM56_002220"
     CDS             complement(9282..10682)
                     /gene="lacG"
                     /locus_tag="SCM56_002220"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEG1110968.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            10919..11704
                     /locus_tag="SCM56_002221"
     CDS             10919..11704
                     /locus_tag="SCM56_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEG1110969.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            11707..12399
                     /locus_tag="SCM56_002222"
     CDS             11707..12399
                     /locus_tag="SCM56_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEG1110970.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(12673..13044)
                     /locus_tag="SCM56_002223"
     CDS             complement(12673..13044)
                     /locus_tag="SCM56_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HEG1110971.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
BASE COUNT         4038 a         2703 c         2173 g         4266 t
ORIGIN      
        1 taaaatatcg tttgcaatta gctttcttct tcgaaccttt tcacctgatt cagtaaagta
       61 caaaattgct aacttgaaaa ataaactatg attatttacc ttaattggtg atatatttat
      121 agcatcaaaa ttgcaatacg gtaattcttt ctttaaatac tcacccagtg aaaacagagg
      181 atagtgttga ggagcaagta cacaaatatc tttttcatga atgccgcttt caatcttttc
      241 ctttattata ttttttatta tgttgggtaa atcctcctta gtaactttgc aatcataact
      301 tataaaacca cggtcgtttt tattattccc tctgctttca atctcgtaag gacaactctg
      361 aaaattagag tagaagtcta ctaatctctg attagacctg taacagccat gtagcgtctt
      421 tgattgaaaa gtgtgactag ttagcatttc tagttcttca actgatttag ctataccatc
      481 taatgatcca taaattgctt ggtcaatatc tccaacaaac attgactgaa tttgagggtt
      541 gttttgaagt aatttagcta ttacattgta ttgtaattct ctagtatctt gaaattcatc
      601 cacataaatc gcccttatat tactagcaat attacgtgca acgttactgt ttacagttaa
      661 aattttatac gctactagta aaatcatatc aaaatcaatt tctttatttt tttccaacat
      721 agtgtgatac atttcagaaa cttgagtata ctgaacttca ttaattttaa aatcatgaga
      781 aagtgacaaa tcaattttgt tcagttcata cgttttgaga tttaaattca gcatattatt
      841 aatttggctg acgtaagctc tttgagtata atcatcaata attgtaaatc cttttgatac
      901 tcttgagaga ttcattttaa aaggataaat aataaattca agacaaaact gatgtattgt
      961 ccccacccaa atattaatat catcatctat actaagtaaa cttaggcgtt ccttaatttc
     1021 ttcagcagct ctattagtgt atgtgatagc aattattctt tttagtgatc catcgtgttt
     1081 aattgcttgg taagcaattt tccttgtaag tacccttgtt ttcccactcc caggacatgc
     1141 atttacataa aggttatctt cactttccac aacatctttt tgttctaaat ttaaatcatt
     1201 tacgattttt tcgaagatat ttgtcatttt gaaacctcag cataaagtat ttttgtgtaa
     1261 ttttctatct gattgattag atacacaaca tcctcatctt taccaaagta ctctaataaa
     1321 gaaattattg ctatattcaa atcagctgta tcacttgttt ttaaactagc tgataaagct
     1381 gacaattctg tagtttcctc gtacaagtcg attgtatgga gtagaatttt tttataaatt
     1441 gagagatcta ctatttcctg acatgcaaaa gcaatagcat taagaatata cttaggaatt
     1501 ttgattaaaa agtcaatttt ttcacctaac tcaattgcaa accacccctt accaacatga
     1561 ttggctagtt ttaacatcgc taaatttctt tctaaatatt gttcactttg tatttcttct
     1621 ttataagcag ttattgtttt tttatctttg aatgcgtcat ctataattgg actgacataa
     1681 gttagaatat tttctttatt cacatctaaa aactcaattt caaatgttgt attagcataa
     1741 aaacaattaa cccaattgtt ttcattaaaa agggaggata atttttcttt tcttgcttga
     1801 cctttctctt gtgcagtgct tttatacaat cgatgttctg atgggattgc ctgttcatct
     1861 aaatcagtta caatagcaca ctttctctgt attctatcat cgtcaaacaa gctagcgata
     1921 tattcaaacg aagtactacc aacattaatc aaacctattc caagttcgtc taagtttata
     1981 ccaaataatt tttttactat ttgaggaata agtatttctt cagcatctcc ttcaactaag
     2041 ataacacctt tagaaaaaag caatccattt cttttaacat ctaaatatct ctctactgcg
     2101 tttattagat ttagattgtt cttttttaac ttttctttag caaatgagtt cagattattt
     2161 gctggattca taacgataga tttgccatta aacgatttta atacattcat ccgagatatt
     2221 tcagacactt cagctaaatg tacagaatgt gtggtcatga gaatctgagt atagtttttt
     2281 tgaagattta atccttcgaa cagagttttt tgaatatgat gatgaatatg agcttctggt
     2341 tcttcaatca acataatatt aagtagttct cttgaacgac acgcttcaaa ttcgactatt
     2401 ttcaaagcta aataaatcat atttagatgt cctaacccta gtaagtctaa gtcgatattt
     2461 tgttctggct tcattgaaag aaattttgct aaagaaccca tatcatctga catagttgag
     2521 gaaagaagaa gattaggaga atacactgaa ccaattatgc tatttagttg ccgattaata
     2581 ttttcgccaa ttttcctaat ttcaggaaca ctggtaatag tctcatttaa ttgctgtata
     2641 attgttttta atgactcaac attatctgat gatatttttg attcaattgt ttccatgatt
     2701 cttttgactg ggtttcgatt gtttttcatt tctctaagaa catctcgaag agcgtcaaca
     2761 taaacaaccg aaatatgctt gaaaatatct gacatgtcaa ttcttgagcc caacagagct
     2821 tcatcgtctt cttcaggatt actagctata ttgtcattga gtcttcctac caatttatca
     2881 taattttcat tgatacagaa atttagattt gatttagatg tgtagtaaaa ttcatagtcg
     2941 gatagacgaa tagaatttct aaattcgtta aattgatgag agtctaattc agtggaaata
     3001 tcaaataatt gctttctaat agctttgtta ggtctaataa atagagaaat actacctaca
     3061 cctttatctg catttgaaat caattcttct aagattgttt gactttcaga atttaaagaa
     3121 atggaagcac aaatctcatt gtcaaattct tcttcgctga ttccttcaaa tgtcgcagag
     3181 ataataatcc aatgcccttg ccacccattt tgaattccat gaaaaaaatc tgattctttt
     3241 agtgttttac tactatagta ataactatca tctaataaga ttctcaatgc agtaagagca
     3301 ttggttttcc ctgaatcatt ctcacctata atagtatttg tctcattatc gaatcgaaag
     3361 atttcggatt cataatttct aaaatttcga atttgaagag tagatataaa cataagcaga
     3421 tctcctaact atttaattaa tatgattata tagtaagttt atcataaatt atgttagtaa
     3481 aagttctcat tataagggag cgtcaataat tttgtgtaaa tgattcttcc ctactgcaga
     3541 ttgtttctaa ttctcaatca gttggcttag catttgttcg agctcgcctt cggcctgttg
     3601 gaaaccttta tgactgcgac ctaggaattt ttggttgtaa gtatcaaaag acgagactaa
     3661 gaaacgttcc atcgactctt cattttggaa ctgttccttg cggtggctgt atttcttgat
     3721 ttgtttatta aaggattcaa tcaagttcgt agaataaata ctcttgcgta tggccaaggg
     3781 gaaatcatag aaagtgagca agtgatcgtt cgaaagaatc gattcaacca cttttggata
     3841 gctggttttc catttttccg caaaagtacc acgtgcttcc aacgccacct ctttagatga
     3901 ggcttgatac accattttaa aatcatcaca gacttcctta cgatcatcga cacgcacttt
     3961 atgactgata ttacgggaaa cgtgtacaca acaatgttga aaacgagctt tgggatagaa
     4021 ccgactaatc gctcctacca tcccctttaa gccatccgtg atgaacagga ggacattttt
     4081 caaaccgcgc tcttgaaggt ccaataaaat ttcctcccaa atcgtgatgg attcagtcgg
     4141 tgcaatcgca tagctcaata cttcctttga tccgtccggg cgaatgccaa ctgcgatatg
     4201 aatagcttcc ttggcgacgg ttttccgctt taacggaata tacgttgcgt ccatataaat
     4261 agcagcataa cggtcatgaa gctcccgccc attaaacgcc gttacctctt ctgtaaatga
     4321 ttttgttata ttggacatgg tttggggcgt gtagtaatgc ccatacattt tctcaatcaa
     4381 gtctgcgatt tccgacatgg taataccttt tcggaagagg tgaatgacgg tctcctctaa
     4441 cgtgtcattc gtccgtctat aagcaggaac agtctgttgc ttgaactcac cattgcggtc
     4501 gcgcggaatc tggagatgaa gttccccata ctcggtcttg accgtacggt cataggagcc
     4561 gttacgagaa ttacccgtgt taaaaccaat gcgatcatac ttttcgtaat ctaggaaagc
     4621 cgttaactcc gttttgagaa gcgtattgac tgctttctcc aaatggacac ggaataattc
     4681 attcaaatcg cctttgttcg ctagagtctt tagaatttct gtagtaaaat cgttcatagg
     4741 gaagtcctct cttctgtgaa tgtgttgtgg taactctatt ctacagaagg gacttccttt
     4801 tttctattta cacaaaatat tttacactct ccattataac aattgggtta aaaagaaaat
     4861 agatagtatt ctacctcata tttatttgaa agtaatatat aaaagacgtt caaattcgaa
     4921 cgcctctcgt taaccaaata ttggattcag aatctagttt tcatatataa acagagtatg
     4981 attaggtgca aaaattgcac ttttattgca cttttatttt tatttatagt gttttactgt
     5041 tctgtatatt gttttaactc ccttgatagt atcgcattct gttttataca ctttacgttg
     5101 aaaacagagt gggaataaag agccaacata atatggaggg ggaatcccta taaatcaaca
     5161 ttcttcaata gctaaaatgc ttgattttca ttaaaaataa gatggttccc taccaaaaga
     5221 gactataata taagttaatt tgtttcattc ttatcataaa aagaagttct cgaaaaacga
     5281 actgttttcc gagaactttt ttctatccat ctaagtgctc aaatcatatt cacgacggaa
     5341 ttctaaggtt accctttctt aaacaaactt tatgttatct gcacaacttt ttgcaatttt
     5401 ttcattgctt cttacgaaat cgttgaactc tctttagcat aatacaaagt gtagatgaag
     5461 tctggtgctt tactcgtttt ggtaaaaatg actggaaatc gtttctggtt atttttggca
     5521 aaggcgaggt gcaggttcga ttcctgcctt ctacgctaac gatgtggctg aagggaacct
     5581 ccgatcttga atcgaaacga tccgcagtct attgtttcac caatgaaaca aaatcaacgt
     5641 tcgtccaagc atttcctgaa gccttgctat aaaccccaat ttcacaaaca cctgttgtaa
     5701 ttggaatgtc tttgattgta atttttgtcc attcacttgt tttaacaacc gcagtattta
     5761 gttccgctcc accataattt ttgatatata actgattagt atcaaactct cccccagaca
     5821 ttacataggc actcaatgtg taagttcctt tgcttatgcc actaacattt tgataagctg
     5881 atgcctcgta tgcttgatcc tgccaaaaag ttagtttgta gtccccaaaa aaagtgcccg
     5941 cttccgtttt gatcgtaccc ttagcggttc cttgttgaaa ccaggtttgc caattggaag
     6001 gactggtcgt tggtccatcg ctttcgaaac ttgcattttt aactagattt atttgcgccc
     6061 ctgcttgttt aaacgcacgc aagcttggca aggcgtttcc agcaaaatca aacatcgctt
     6121 ggttgtccca ttcgtttccc atagtggatt gaacactgag ataattcata cctgcttgtg
     6181 ttgcccaact cacatttccg ttgtaccaaa gaggttccca atagaagaag cccgccccgc
     6241 gattattcgg tactgcctga atccgatcgg caagatccag caaatactct gcttgtcctg
     6301 tcggtgttgg ttcatatccc cccgcagctg cttcggcttc accaaaagca tttggcatag
     6361 tatcggcatt cgctgttgta tgcccataag ctgtttcaac tacgatcact tctttaccat
     6421 accgttggct gatcgcattc atattggttt gaagttcatt cattgaaccg tgccaatacg
     6481 gataatacga cactccaatt aaatcaaatg acacacctcg actcgtgatt tcatcaaacc
     6541 accaagtaaa tgctcctgta tcgccaccat cagctaagtg gagcattact ggagttgttt
     6601 taggttgagt gtctcgaaca gcttggatac cagacttcaa aaaagcagcc aatcgatcaa
     6661 attctccccc gccttctccc caagacttgc cataaggcca gagcattccg ctattcagtt
     6721 cattgccgat ctgaaccata tcaggataaa cgtccagctg cttcatttcc gacagaacgt
     6781 tctttgtata gtcgtacaat gccgtattca tttcttcaaa ggtcagacct tgccatgctt
     6841 taggaagatt ctgtttccca ggatcaaccc aaaaatcact gtagtgaaaa tccagcagca
     6901 ccttaagtcc cagatttttt gctcgttttg caaggtcaat cgtcgtttcc agatcatttg
     6961 tccctgctcc ataactttct ccattactat tataaggatc ctgccacaaa cgcagtcgaa
     7021 catagttgac accattattt tttaaaatct ctaaggcatc ttttgaaata tcgtcttcat
     7081 aatattttgc accagatttt tccatatctg acaggatcga aatatctgct ccgcgaataa
     7141 attcgctggc agatgcttca acagtttttg aaacagcccc caaagcccct aacacagctg
     7201 caaataaaac agctttacgc aaccaaattc ccattttcat tatggctcct cctttattga
     7261 aattggttca gaataaactg aacagctttt tcaggatctc tcgttaatcc gatatattct
     7321 gctccttttg tcgcaaccag cttgattcct aagcgatcgg tgtcttcttt gatatcatca
     7381 tagtaagaat tgacttgcgg tgctaagacc accatatcaa aatcctgcag catttcgtaa
     7441 tgagaaccat aagcacccgc cattgcactg atcggtgctt tcgattcaga tgcacctttt
     7501 tttaaagcat tagctaacat cgcacttgtc cctgcgccag cacaaagtac caacacattg
     7561 atttctttat ttgcatcgct gataacttga ctcgctgcca caggtacttc ctcacgtaca
     7621 tcggcagaag caccttcctc ggttgcgatc acctctgctt tttgtgcttc tttctctact
     7681 aagacagcat cgtatgcttt gcaaaatggt agatagatca ggaagtcaac gacgattaga
     7741 accgctgcta gaacaaaagc taagataccc atgcccgttc ctagaaccaa accgatcggt
     7801 gctggagttg cccaaggcaa aacgtacata aagctgttca tgcccaatac atcgacaaaa
     7861 aacttgaaca ggcaaacgtt tacaacaggt gttaacaaaa atggaataaa aaaataaggg
     7921 ttcaaaatca tgggtgccgc aaatagcaat ggttcgttga cagcgaaact tactggaata
     7981 aaggaagctt tcccaactgc cttcaactgc ttagacttag caaagagtag gaagataaaa
     8041 ggaacaacca atgttgcccc agttcctccc atagttccta caaaattccc taaacccacc
     8101 gtcagtactt tagaagcttg ttcaccggaa ttgaacaatt gcagattggt ttccacattg
     8161 gcatagataa ttgccgcaat tgccggctcc acgatcgacg gtccatgaac accgacaaac
     8221 caaaacagcg ccatcgctcc ccagataata gcgattccta agtacccgtc agctgctgta
     8281 aatagcggct gcagcaccgt aatgattgct tctgcaaatg agatttggaa gatttgtcga
     8341 atgcctaaat caattaaaac ggccgcaaat actgaaaaag agaaaggaaa aatatctcgg
     8401 aaggtttgcg aaatcgttcc aggaacttct ttaggcattt taatggtaat atctttcagc
     8461 acacaaaact tatataaatt aactgttaaa aatgcagcga caaaggaagc cagtagtccc
     8521 tttgtcccca tatattccgt tgaaaatcca ccttcaatcg gagagacgct taacaacagg
     8581 aaacttacca cagaggccag catcacagag gtggtgttga ttactttgtt tttcggtaag
     8641 cgacgattca tcgaatcagc caagcagcga gcagtggttg ctgaaacgag taaccctaca
     8701 acacccatcg aataattata gactttccat agccatgtgg agacagattc aggccattgg
     8761 taaccaaaaa tctcaggaac cgcagcaatc aaaatgaaaa tgcttgaaaa taaaatagct
     8821 ggcatcgctg ttaaaaatcc gtcacgaacg gctcctaaat aactattacg tgagattttt
     8881 tcaaaaaatg gtttcccttt ttcgatttgt ttaatgattc catccatagc gttcttctcc
     8941 tatccttgat tctgtctgta cagtacaata aagtcttgaa tcagatctct cagcaataaa
     9001 gtggtcatta aatgatcctg cccatggata aaaatgaatc ccatctccat ctctttccca
     9061 gatgcttctt ccgctaaaat ttttgtttgc gaattatgtg caagggttaa attttcgtcg
     9121 gcttctttca acgccgaatc aacatgttca aagtttcctt gtcttacttc tttcaataat
     9181 ttcaataatg tactgcgagc gtcgcctgaa tatgcaacga tctcaaaacc aagcatttgt
     9241 aattcctctt tattcatctt ttcttcctcc attatcactt attaaataat cgttttggtt
     9301 tcactgacca acttgtacca gtaagctgat tcttttgggt aacgcttctg ggtttcaaaa
     9361 tcaacataaa acaagccata ccttttgtta tacccatttg tccatgagaa aagatccata
     9421 agcgaccaca aaaaataccc cttcacattt accccggctg ttatcgcctt gcttaaggat
     9481 tccaaataga ctctcaaata gtcgattctc ggctgatcca taataatgcc atcttcaaac
     9541 tgatctttat accccatccc gttctcagtg atatagattt tgttgtaatg tgggtaatcg
     9601 cttttaattc gcagcaacaa gtcatataga ccttctggat aaatcagcca gtcccaatca
     9661 gttcgaggaa ttccttcttt ataaatacgt tctccgattc ccttcacttt ataaacagag
     9721 gtcccttttt cacccgtacc gttatgatgg attgcatttt ctccatcgta agctttgaca
     9781 aaatggcatt ggtagtgatt gatccctaag tagtcattac gcgtggatgc ttttttcaat
     9841 tcaacgaaat cttcttcagg aaaatgatag ctggcctgat ttgcttcaca tatctcatca
     9901 agagcagtca aggtttcagt tgaataatag cctaaatagg tagcatctaa taagaaacgg
     9961 atcgacaatg catcgtctaa aaaggcagca tgcttatctt ccggcgcgtc tgttgccgca
    10021 tatttcgtct ctaatgagtg aactacacca atttcacccg gaagttcgtt ttctttgaaa
    10081 tagttcacta cacgggcatg agcaaccatc atattatgaa gacaggcgac gatctttgta
    10141 aaatcatatt tgattcctgg tgggaaaaca cctaacaaat attgattggt tgcaactgga
    10201 tagatttcat taaaggtact ccacaccttg acttctttga attcatgaaa acaaaaaatg
    10261 gcataggaaa caaatgcttc tattgtttct cgattcaaaa aatctccatg gtcaaacaaa
    10321 cgtttagggg tatcaaaatg atgcaacgtc acaaacggcg ttacgtttct tttcttacat
    10381 tcttcgaaca cacgatgata aaaagcgatt ccttctggat ttggtttccc agctccatca
    10441 ggaaaaatgc ggctccaagc aatggataat cttagaccat ttacaccaaa acgttcacac
    10501 aattcaatgt cctttgcata ttgatgataa aaatcacttg ccggatcggg actgaatcgc
    10561 ccttgttcct ctaaaaagtc atcccaagca acagcgcctt tgccaccttc cttagtggct
    10621 ccttcaacct gataggctgc tgtggctccg ccaaaaataa aatcttctgg taatttaagc
    10681 atgtacatct ctcctcaaat ctataatcag ttttcgctct ttctaactga ttgcattgta
    10741 aacgcatcca aaacatttgt caacaaaaac aaacacaaat aatcataaaa accagaaaac
    10801 agcaataaaa tctattgtaa caaacataaa aaccacccta aatgtttact tttgttcgaa
    10861 ttagattgac tgttgtccgt ttatatgata gattatcctt aaattagagg tgaagacaat
    10921 gttgaaaaaa gaaagattgc ttactatcgt agaaatggtc aataaaaaag gaatccttac
    10981 cgttaatgaa atcatcaatc aattagatgt ttctgatatg accgttcgca gagatttaga
    11041 tgaattagaa aaatcgggca agctgctgcg tgtccatgga ggggctcaaa gtctttccta
    11101 cactttagat caagaacttt ctcacactga aaaagccact ctgcagattc aagaaaaaaa
    11161 agaaattgct gccacagcag cctccttgat tcacgaggga gaaacgattt tcttaggacc
    11221 aggtacaaca atcgaactat tggcgacgca tttactgaac aagaaaattc ggatcattac
    11281 aaataattat ccagtttttg atattcttgc tcaagcagat ggaccagaaa ttctactgat
    11341 tggcggtgat ttccggaaaa ataccggcgc atttgtcgga ccgatcacca acgacaattt
    11401 aagaagacta aagtttacaa aagcatttat cagttcaaat ggcgttcaca acgaagcgat
    11461 ctctacttac agtaccgaag aaggagaagc acagcagatc gctctaaata attcccgtaa
    11521 gaaatatcta ctcgttgata ataaaaaatt caatcgagat gatttttatg tgttttataa
    11581 tttgcatgat ttcgatctgc tcattacgga tcgccagatc tcgcgggagg ttcaagagca
    11641 ctatgcacaa tatgtcgatc tgcaaattgc tgattcaacc gaaaatacca ctataggagc
    11701 gtaactatga aagggattat atttgatttc aacggaacaa tgtttttgga ctctcatctg
    11761 catgaagctg cttggctgca tatgatacat gatcatacaa aagatggttt atcagacgag
    11821 gatattttaa aaaatattca tggtcggacg aatacggaga ttttgaaaca tttcatttca
    11881 gagcatttga ccgcctctga gatcgccgta ttgtcagaag aaaaagaaag ctattatcgt
    11941 atgctttgct tgcaaaatga aaacgagctt cagttgacaa aagggcttga agcgactctt
    12001 gaccagctga ctgaccaaca gattccatta acgattgcca cggctactgt caaagagaat
    12061 gttgcttttt attttgatat tttcgattta gcccgctggt ttgactggga caagattgtc
    12121 tatgacgacg gctctttccc tgggaaaccg caaccggata tttttcttaa agcggcagac
    12181 aaactgagct tagctccggc tgattgtctt gttatcgaag atgcttactc aggattactt
    12241 gctgccaaac gtgcggggat cggaacaatt attgcaattg atccattcgg taaaaacaaa
    12301 acagtatttg aacaggcaca gctaaataaa gatggaacca tcaaagattt tactggtttt
    12361 tggacagagc acctgtctgc cacatccgtt aattcttaaa cgattcaact aactaccaaa
    12421 gaaaagcaac tgaaatgcac acctcctttt ataaaaaact gccagtgaaa cgacatgaaa
    12481 gatacactgc tatatcctta gtgaaatata gcagtgtatc tttgactttt tcattctttt
    12541 aaccatatga cgcgttcatc tctatctata attaacatta aaaatagtgt tttcacatta
    12601 tttctcaaac caatcttaaa aatcatcagg agtacttttt tgatatactt ttattaatgc
    12661 tgggcgtaat ggttaaaaga tttccactag ttcctctcca ccaagctgag ttgaaaaatg
    12721 cttatctgta aacaacatag acaaagagga aataaacgtt tccgtatctt tctctgtccc
    12781 aaggatatta attaattttc ctactaggta agtatcatta aacttaagat ctgccttcac
    12841 atcaaaagtt ttgataaaag cttttatcat gcgtacaatt ttgtctttat cgccataatt
    12901 gatttcacca ctcatatcaa tatagtcact aatccctgaa gaaataatgg caactgcttc
    12961 ttctttcgcc ttatcagata attcatttaa tgactgggat tgattgatga cttcttgagt
    13021 tttatctgtt acgtaatctc tcattatcct atcggcatac tccgcaaagt aatcttgaaa
    13081 aaattcttct acagggtaac caacttcgtt ttcccaacca ggagaaattt cattatcagt
    13141 acttattaaa tctattaaaa accaacccgt tctctgagtg
//
LOCUS       DARBDV010000081        13060 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000081 DARBDV010000000
VERSION     DARBDV010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.082"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            66..539
                     /locus_tag="SCM56_002224"
     CDS             66..539
                     /locus_tag="SCM56_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HEG1110972.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            548..775
                     /locus_tag="SCM56_002225"
     CDS             548..775
                     /locus_tag="SCM56_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1110973.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            1411..1782
                     /locus_tag="SCM56_002226"
     CDS             1411..1782
                     /locus_tag="SCM56_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110974.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2038..2280)
                     /locus_tag="SCM56_002227"
     CDS             complement(2038..2280)
                     /locus_tag="SCM56_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEG1110975.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(2312..3214)
                     /locus_tag="SCM56_002228"
     CDS             complement(2312..3214)
                     /locus_tag="SCM56_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEG1110976.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(3227..3415)
                     /locus_tag="SCM56_002229"
     CDS             complement(3227..3415)
                     /locus_tag="SCM56_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEG1110977.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(3429..3992)
                     /gene="amaP"
                     /locus_tag="SCM56_002230"
     CDS             complement(3429..3992)
                     /gene="amaP"
                     /locus_tag="SCM56_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEG1110978.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4030..4923)
                     /locus_tag="SCM56_002231"
     CDS             complement(4030..4923)
                     /locus_tag="SCM56_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG1110979.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5001..5924)
                     /locus_tag="SCM56_002232"
     CDS             complement(5001..5924)
                     /locus_tag="SCM56_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEG1110980.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(5973..6323)
                     /locus_tag="SCM56_002233"
     CDS             complement(5973..6323)
                     /locus_tag="SCM56_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEG1110981.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(6356..7258)
                     /locus_tag="SCM56_002234"
     CDS             complement(6356..7258)
                     /locus_tag="SCM56_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEG1110982.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(7251..8108)
                     /locus_tag="SCM56_002235"
     CDS             complement(7251..8108)
                     /locus_tag="SCM56_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEG1110983.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            8442..9251
                     /locus_tag="SCM56_002236"
     CDS             8442..9251
                     /locus_tag="SCM56_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG1110984.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            9291..9788
                     /locus_tag="SCM56_002237"
     CDS             9291..9788
                     /locus_tag="SCM56_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEG1110985.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(10173..10246)
                     /locus_tag="SCM56_002238"
     tRNA            complement(10173..10246)
                     /locus_tag="SCM56_002238"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10210..10212),aa:Ile,seq:cat)
     gene            10433..10756
                     /locus_tag="SCM56_002239"
     CDS             10433..10756
                     /locus_tag="SCM56_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110986.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            10920..11174
                     /locus_tag="SCM56_002240"
     CDS             10920..11174
                     /locus_tag="SCM56_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110987.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            11244..11489
                     /locus_tag="SCM56_002241"
     CDS             11244..11489
                     /locus_tag="SCM56_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1110988.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            11565..11930
                     /locus_tag="SCM56_002242"
     CDS             11565..11930
                     /locus_tag="SCM56_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110989.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(11991..12554)
                     /locus_tag="SCM56_002243"
     CDS             complement(11991..12554)
                     /locus_tag="SCM56_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1110990.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT         3793 a         2433 c         2197 g         4637 t
ORIGIN      
        1 aaatagttgt tttacaataa ttattctgct atattttagc tatcaaagat aaaagaggga
       61 taaaaatgaa atttaaaaca ttgctattaa aatttgtcgt tttaggtata agtgccgtat
      121 ttattttgtt cggcgtactt ttttatactc agatcgctac aagcgaaaaa ggattcaatt
      181 ttgattggca gaccacatta tttgtttctg tcatttactt tgcccttctc ctagggatcg
      241 ttattgctta tttcttattg aaactattga aaaaaatcga agataaccaa gctttttcca
      301 ctcaaagctt aaacatacta aaaaaaatcg aacgaaccat tttgtttatt ggtttcgcct
      361 tttttggctt acttcctaaa tttatgaaag tagcggattt agacgatgct ccaggtttga
      421 tgttggtagg aatcgctgtc gtattcattc cttttgcagt atacacatta acaagcgtgc
      481 tggaaaaact gcttgaacat gcgattttac ttaagacaga tcatgatttg acagtgtgag
      541 gtgcgacatg gcaattatcg ttaatttaga tgtagtatta gctcaaaaaa aaatgagtgt
      601 gactgaattg agtgagaaag tgggcattac gatggcaaac atctctattc taaaaaatgg
      661 aaaagcaaaa gccatccgtt tttccactct cgaaaaaatc tgccaagttt tggagtgtca
      721 accaggtgat ttgttgacct ataaaaatga ctcgaatact ataccagaag aataaaataa
      781 agtagaacaa acagtaaata aaaaataatc tgaatgatag atgtgtgaga cttttagaag
      841 ttatttacgt ctattattca gattttttat ttcgattgaa taccgtttta tctataaata
      901 agatatgtac tgactcatac aaagggggcc ttttcttctc catgtactat cacttttcct
      961 gatacaactg gtttcctgtt acgttgtagt tggatctgct tttagtataa tactttattc
     1021 attccaccag actgatagca gtttgagcag tctaaataat catgtagtat tacaggccat
     1081 tctgtttcat accttgaagt gcttaaaact tcagctaatg gcccattgtg ttaactctta
     1141 ctccattctt ttttagtaag ctcccatata tactgtaaag ccttctatat attatgtcat
     1201 gttgcttttt aacatttact atgtagtttt gataaaatga tttgaataac agtgatggac
     1261 aattcttttt tatttttaac attattttga taataatgat tatttattta aaaatacatt
     1321 agataacatt tgttacgaca cgaaaaagct tcacaaacaa atatcgttat tttaaaatga
     1381 gggcgaactc acacaattag gaggaatagt atgccattta ttgtttactt ttttctttca
     1441 ttactcgtga tctatattcc attaccaaca tttttcctgc tcgtcaatcg tctagcagag
     1501 caacatgata cgattgctgc tcttacgaaa ctttgtttac ttctatttgt ttgtctagat
     1561 tactttacga gctattattg gctaaaacag acaagagtaa aaaagagata ttatttcttc
     1621 cttgtctttt tcaatctctt tgagtttctg atccattgct ttttacttca tcagtatcat
     1681 acgatccatt tgtggatcat ctgggttttc aaagtggtcg tattattttg tatgttcacc
     1741 tttcctatct caagaaaatt tagggaggca ttgttttatt aaagaagctt ctctgtatca
     1801 ataaagacac atttaaggaa aaacttttaa aaatctaatt gggaatttgc ccgcataaat
     1861 aagaggctgt gacaaaagtc ttgtcacagc ctcttattcc gtataaacag tgttcaaaag
     1921 cttgcatctt cgataatatg cccaaccaaa tgaaaaatat gagaagctat ttttgtttgg
     1981 ctgttcttat tacttgatga agaacagctt tttcacgacc tctttttaaa agtcttttta
     2041 cagttttcga gcaaagaatg atacgattag tactaaaatg atggcaccaa tcaatgatgg
     2101 cacgattgcc attcctgcaa tacttggtcc ccaagaacct aataatgctt gaccaagtgc
     2161 tgatccaact aaacctgcta cgacatttgc aataatcccc atcgaagaac ctttattcgt
     2221 gattgctcct gcaattgctc cgataattcc tccgacgata agtgaccaaa taaatcccat
     2281 gtttcgttcc tcctaatgtt ttacttcctt attactgaac ccgtggttct gtattttctt
     2341 ttactttttg tgcgccgctt ccagctgctg attttacttt atctgtttga ttcgaagcaa
     2401 attcaccggt ttttttcgct gcgtcagtta ctttgtcttg caccgtttct tgatcttttt
     2461 caaattcttc ttttgttttg atgtccacta cgttagcgtt gacttcgatc acttctaaat
     2521 gtgtcatatt ttttacttct ttgccgatta cttcttgcat ttcttcaaag attttttcga
     2581 tatctttgcc atattccaca atgacatcca tatcaactgc tacttgtttt ttaccgactt
     2641 ctgtttcgat cccagccgtt acattatctg tattcacaag ttttcctgcc acattagaga
     2701 agaaaccacc gtttgcagat aataaaccgt ctacttgttc aattgcgatt ccgatgattt
     2761 tttgcacaac tttatcttca taattcagct caccgcgcat tgaatcttcc tgttcagaat
     2821 catgctttcc ttggttattt gtatgatcag aaccaagttt tccttgtccg tttgtgtggt
     2881 cagaactaag tttttcttgt gctttcgcat ggtcagaagg atgtttttgc tcttccacag
     2941 gtttagaagg atcgattgca tcatgttgtt ctggaacttt agattctgga gttcctggat
     3001 gatgaggtgc atgcttttct gcatcatgtt tcttctcttc tactccttgt tgaacgttag
     3061 ggactactgg atctggatgt gcagatactg gtgctgtttt gttcttttca tcgttcacac
     3121 catttgattg gtttggtgtc gttgggtctg gatgtgcaga cactggtttt tggttgttca
     3181 ttttattgtt attatttttg ttattgttat tcatggatgt ttcctcctaa gatttaaaat
     3241 agtgatctaa gattttcgtt tgtttcagat aaaaaccgat agcgacgccg atcgctgcca
     3301 tgatcaagat caataaggtc ttgaaaaagc ccacggtgat ccataaaacg gctaatatca
     3361 atccaagtac cccaaagatg atagggagct tgtatgtcga aaatagttct ttcatttttg
     3421 gtccccccct attctactcg tttgcggtca ttgcttcgtt tagctggttc ttcaaagcct
     3481 gtgtatttca ccgatacttt cactggctct tttgttccca atatgtgttg tacttcattt
     3541 tcgatttctt tcatcaatgt tcctgtttta ccaatgagtg ctgaagttcg tttcaactcc
     3601 ccttttacat tgacggcgat cttgtttttc gttgctttga catttacttt tggtgaatgg
     3661 atgaattcat cttcatgcaa gtgagaacga accattcctt caattgcttt tttatccaac
     3721 gtcaactccc catcaccatg tttcagtaga aagtttccga ttgtttttgg ataaaacagg
     3781 aaaacaagta acgtgataat tgtcaaaata gcaaatgcaa cggcaagcca aaatacatat
     3841 tgttgcacgt atgaattagt gatggaaaag aatcgaaact tctctagctg aaaaggcaat
     3901 ggataggttg cacttaatag tatcgcaaac aaaagcacag atagcaaaag taatgttccg
     3961 atcactccga tcgtttttac acctttattc attttcgcac ctcctcatag gggtgaaacg
     4021 atcaacttct tataagttga tcgcttctcc accagttact ccgtaaattt gggctgtaac
     4081 ataacttgct ttattcgatg ctaagaaaac ataaactggt gctaattctc ctggctgacc
     4141 tgcacgttct agtaaacttt gctgaccaaa ttcagggatc gaatcagaag gttgtccttc
     4201 gtctagttgt aatggtgtcc agattggacc aggtgctaca ccatttacac ggattccttt
     4261 aggtgcaagc tgttttgcta aaccgatcgt aaagttagcg attgaagctt ttgttgctgc
     4321 gtaatctaat aaatgttcgc ttggattgaa tgcttgaaca gaagttgttg ttacgattgc
     4381 tcctcctgct gggatatgcg gaacagcttc tttcactaaa gcaaacatag cgatgatgtt
     4441 tatcataaat gtgtctttga cttgctcaag tggtaactca gcaatagact gacaagaaat
     4501 ttgctgtcct gcgtttagta ccatcgtatc tagcccgcca agttcactca cggctttatc
     4561 tatgatttct ttaggtgctt gttcgtcacg aagatcaaaa ggtagtaaga caatcttacg
     4621 tcctgcatct ttcacgtatt ttgctacttc ctctgcatct ttttcttctc ctgggaagaa
     4681 ttgaagagcg atatctgccc cttctctagc aaatgcaatc gctgctgcac gaccgatccc
     4741 tgaatctcca ccagtgatta gtacacggcg gttttctagt tgattattgc cttcatagga
     4801 ttcttctcca caatcaggaa caggaatcat attctgctgt aaagctggtg tatcctgatc
     4861 ttgtttagga aatttctccg tatggtacaa gtttcgcggg tcgattaatt ttggttctgt
     4921 cattgctgta ttcctcctag tttttaaatt gtattgccaa aacaaatttc tttgtttgat
     4981 acctgtttta aatagattct ttaatttgta taatctttcc ggtataaatg gtaacctaaa
     5041 actactgctg ctatcaaaga gccaaagact acgagtataa agcctagagc agaactttta
     5101 cccggtaatc ctcctgtatt cattccccaa atccctgcga acaacgccgg aacggaaatg
     5161 attaatgcag ctgaatctag atatttcatg atatgattta gattattgtc catcatcccg
     5221 ttgaatagat caccaatact ctctaataaa tctcgataga tctcgatcat tttttctgtt
     5281 tgtcgctgtc tcaaatggac atcatagagt agctgttcat cgtctaaacg tttgatgaaa
     5341 gtttcagaat tccataattg atccattgtt tcccgttgat ctcttaaggt atgatcgata
     5401 tatacgatgt ctctttcaag atctgcctgt ttatacagtt cttcattttc cgttgtttta
     5461 cgagctgctt tatccaaagc gtcaattcgc tttttcatct ctcttagttc tttcacgtaa
     5521 tgggtgtaaa tatctaataa tgtatacgtg atgatctttt caaacgagtt taaatgattt
     5581 ccatgctttg tcagcagccg atccacaaat tcggtttttc tatcgataca agtaatcaag
     5641 agattatcgg aaagtacaaa agatattggc gttaattttt tttcgatacg attatctgac
     5701 tgatctagat taatcactac caaagaaaat ggatgctcta gtttcgtctc ataaaggcgc
     5761 tccatccgag aaacttcttc cggatattcg gttcctacga aaatatcatc aggaagctcg
     5821 aattcttcga ttgcttgctc aatctcttcg ggctttgcac tatcaagtac gagccaatta
     5881 tatttttttt catctgtttc tattacattc ttttcttcat ccacaaagta gtatctcaat
     5941 gtatacctcc tttttggact ttctcgaaac tattagctga agaagcagtt ctttaatttg
     6001 atattataat cgagtactgc gatttcattt tctaatgaaa agctgtcaag ctgtttgttg
     6061 aagtccatgt tcaatcgttt accgaaataa ttttgctcta atttagatgt atagatgtcc
     6121 aataatggat gctcgatttt agtcgcatat tctccaaatt ccgaaacaaa aggaatgatt
     6181 tgaaaaaagc tgccttttgc aaaagcacct ttgtccctta gaaatggatc ggaatcattg
     6241 ataagcgcga gtgcgatttt tgtattttcc ccaacctttt caattaacgt ctctactgca
     6301 tttttagaca ttgtaatttc cattcctact ccccctcttc agttgttgtc ttctattatt
     6361 taacaactac atctgttttt ggttgaactt ttgcatattt tggatattta gggaatttag
     6421 ctacacaacg gaaagccgtc atccctgcat atttttcgta atatttcttc gttggccctt
     6481 caaaatcctt gctccacggt ttcacatgtc ctgaaaaatg gataatgtaa ggattgttgc
     6541 gagtttcttc gtattcacgt tcacctgctg ctgttggatg tttttttgct tttgccatga
     6601 tatagccttg tgcattccaa cgcggatgaa gtggtaacca acggtcatgt aagatagcat
     6661 ttaatgcatc ttggtcatgg aatcgtaatt tttctggatt atgttcgatg aaatctaata
     6721 ctttttgcgt aatattttca tctaaccatt ttttcacatt gatcagcatc aaacctgagt
     6781 tgaaataacg cattgattta gctgaaattt tcattttttc cagtcgttga tggaacccag
     6841 cgtcttcaac agctgctacg attgaatcac cgaagtcaag acgccataat ttagagatat
     6901 cttgtaaagc gatcatgtcg caatccatat ataagatacg ttcaacttct gtgccgcgga
     6961 acaattccgg aatcgcaata cgataataag ctgtttctgg aatacgatcg ctgattacca
     7021 catttgtaaa gaattcttta ttgattttca agaattccac atctgagttc attcgtgcgt
     7081 ttttcactga gaagcgtaaa agctgtttac tttcaaaatc gatatcatcg tcaatcacat
     7141 agaaccgaac aaatttagac gggttgcaat tatctaatac tgagacaaat aatgcagcta
     7201 aatgtggtac gaattttgtg ttacaacttg cgactactgc tatttctttt ttattcattg
     7261 atctcgccta ctttcgttag agtactgtgt gctaattttt ttaagtataa attggtatac
     7321 ggatgatctt tcaacgtatt ccaaggttta tcatgtcctg taaagtgaac gatcgaaggt
     7381 ttttcatttc cttctttata ctgacgttca tatttttcat ttggtgctgg atgacgttca
     7441 aaaatcagcg aagtctgcat gttccatttt ggatgaagtt gttcccaatc ttcataaagt
     7501 acagcattca aagcatcttg gtcgtgatag atgattcgat cgccattttc actgaggtaa
     7561 tggatagttt tttctgtaat ttctttttta ttccattgat caatgtcaat caccatcact
     7621 cctgaattga agtacaagtc atcggaatcg attcctaatc tttccaaagc tttcacttgg
     7681 cctggatcga taatagcgcc gattgttttt ccatttaacg gctcatcata tagttttacg
     7741 atatcgtcta gtactaacac atctgaatct aagtaaagga ctttcttata atcctcttta
     7801 gctaataagt ttggtaaaga aattcttaag taagcggtag ttgtgatatg gtcactgact
     7861 aggaagtctt cgtagatatc tttttccact gtcaggaatt ggatacttgc attacttgaa
     7921 tatttattta ccgtttcttc caatccttct ttactatatt ctgatagacc atcgtcaatc
     7981 acataaaatt ttatgcgtct tgttttattg caattttcta atgcagttgc gatcatcacg
     8041 ctcaagtaag gagcataatt ttcgtctgaa gcagttacca caggaactac gccgtatctc
     8101 ttctccatca aaatcactcc tttttctaat ctttttctaa cctgttctac atctagaata
     8161 ttcctcattt ggttttaaag caaaatatat gctttgtttt cattgaatat tttaaaattt
     8221 ttttatcaca aaacgcatcg gtaagcgcta actattcgtt tttcatggct ttttattcaa
     8281 aggtattgac caaaaactac tatatatagc cacctttttc ttaagaatgt tttttgaaaa
     8341 aattcataat gacaaagcaa gttagtttca tttttttaaa ttctcattta atcttaaggt
     8401 aacaggaaaa tagatatgta aaaagaaagt aggaagataa aatgaaacta gcagcgattg
     8461 atttagacgg taccttgctt gatcgaaatg gaaagatacc agaagaaaat atccaagcac
     8521 ttcggaaatt tgatgaaaat ggcggaatcg taaccgtcgc taccggaagg aacagcattt
     8581 ctgctaaaga tattttttcc gaattaggaa tcggtggcta tttgatttct tccaatggtt
     8641 cactgattgc tgaaatgaag gatggaaaaa tggcgaaagt tctcagacgt tcaaaaatag
     8701 atagcgatac actgagaaaa gccttcttct atgctcaaga agcgaaaata tccatcattg
     8761 ctagccgaga aacccaagat gatcagctga cattcgatga atcaaatcta gtcaaagatg
     8821 acccttatta tgaacatttc aatttacaga accattcttt tgatcaaatt ttaaatatgt
     8881 tggaagatcc ttctcttagc tatcttaaat tagcattaac agataagagt gaagaaaaac
     8941 tacagaagct acaaagagat ctgcaagcag atggtatcca atcagtattc tctgatccgc
     9001 atttcttaga aatcacgcct acaggtatca ctaaagctca ttcacttgtt tttctggcgg
     9061 attaccttgg aatcgaatcg agtgacatca tggcttttgg cgatcaagaa aacgacattg
     9121 ctatgttgaa ttacagcggg ttgagtattg cgatggggaa tgcacagcga caagtaaaag
     9181 aaattgcaaa ccaagtaaca ctgacgaatg aagaagctgg tgttgcttat attttaaaaa
     9241 acttctatta aaaagaataa aatgacgata tgaatgtagg aggaaagaaa atgttagtta
     9301 ttattgatat gcaaaatcat attcttgatc caaacagcga attttatatt ccagagtcag
     9361 atcaattggc agaacgtatt gctcaaagat tagctaaagc acgccaaaat aatgaatata
     9421 ttctcttcac acgggatata ccagttgaac tgaaggacaa agaagaagaa cgagaagatt
     9481 tgcaattgat ccctcttctt tctccacaag aaaatgaacg agtgattaaa aagtattact
     9541 ttacgattcc acctgaaact ttagctgaaa tccaggaaac tttatttgaa agcaaagaag
     9601 aacaaaaaga gatcgaagta gtcgggattg aaacaaactt gtgtgtactc tctaatctga
     9661 taggcctgca gagtgctttt cctgaagcag acttttttgt tgatccaatg ttagtctcca
     9721 gtagaaaaca cggagaatcc gctttagagc tgttgaaaga tttcaacgtc tctatcgtcg
     9781 aatcataaaa atatctatct tcatttacga tatttaacac acaataaaaa aagcacgcag
     9841 aactgtttta gtcctacgtg ctttttctat caaagtagat tggctttttt atgacacaga
     9901 gtgaaacggc aggctcacga cctctttctt aaatggtgtt caatcagctg accctcggta
     9961 ttagcttaac aaacggcaaa atatgaggaa ctatttttcc gtttgttaag ctaatactca
    10021 ggtcaaaacg ctgatttcac aacctcttcc ttaaacggta ttcaaaagct tgcatctgca
    10081 gtaataagaa cagccaagta aaaacataag aagctgtttt tacttggctg ttcttattac
    10141 ttgatgcagg gcagcttttt cacaacctct tatgtacctt gtaggactcg aacctacgac
    10201 cggacggtta tgagccgtct gctctaacca actgagctaa aggtacatca taaataaaat
    10261 ttatttttgt tacttttcaa ctatagcacg aatgacaaac tctcacaagt ttcaaaatat
    10321 aagtaaaata atattgttat tcttatttct tttacttgtg tcataactat tttgttactt
    10381 taatttttat ttgcttattt tagaaaaaat gttattcttt tatttattct caatgttagg
    10441 agtgattatt ttgccattta ttgcgatttt attcgattta gttgcggcag cagcttattt
    10501 tctccaatta aattatcaaa cctcaggaat tctgtttatt ggaatgattt ttcaaggtgt
    10561 gattaccctt ttgcttcttg tcatgatgct cagctataaa ggaaaaagat atgcccgtat
    10621 tcaaacaaaa gtatttgtta aatatgtttc cattcgctat gggatcatca ttctttcctt
    10681 tttagtgaat gcgatcgtcc tctttttata tgtattgaat tacttagata tcaatccatt
    10741 gattttttct cgatagataa gacacattca gcggaacagt tcctgcttat cagcagaaaa
    10801 ctgttctgct ttttcatttt tcattgatct attcttcttt tattttccta gcatttcatt
    10861 tgtgatgtat gcaataacat gttatctttt attatatgaa tcgttgagga gtccgttaca
    10921 tgattacgtt ggtgagagtt ttgttttggg tgcctgcggt cgcactggtc gcaagtattg
    10981 tttatcttat gaattggaac aaagaacggt tttatttagc tattttgact cttccggcta
    11041 tctactttat gtggaaagtt tttaattaca actactttga acccgatagt gtttttatag
    11101 aagaattatc aggactggtt ttgtctttat tgatcgtcat tctttatttg atacgtttaa
    11161 ataaaaaaca ttgagtacta ttgattgcat gtgtagcttt ctatagtcat tataactatt
    11221 ctactataat gaaaggtgat taaatgagta tcgaagaagc tattttactg gatactttac
    11281 gttgtcgtct gaatttctta agaactggcc gatctttatc tcaagtagct gtttctagcg
    11341 cgttaagtat tcctttattc aaatatcgta agtacgaaga aggagaagaa attccaagca
    11401 tcattgaatt aatctatatc gctgactttt acgatatttc cgttgattac ctaattggtc
    11461 gctcagaaac agcaagcaaa ctgaagtgat tttttccagc cttggtgctg gttttttcat
    11521 ttaacgtcta aggttaacaa tctatatata aaggagacat ttctatgcga ttatggcatg
    11581 aaaaattgat ttcaaagctt cctcgtcaac agcttttagg acaacatcgg gaatgctgtg
    11641 cattaagagg aaatggctgg ggaaagaaac atgctactgt ggattatgtt tttcactatt
    11701 ctccttatag attattccaa tatcatcagc ttgtcatgga tgaaatggaa aaacgtggtt
    11761 acagcccagc accagagtgg aaaaatccta tgtatcgtgg caaatcatgc gaagcatttt
    11821 ttgatttacc tactgtagag cgcaacgtgc cgatttatcc ggaacacgat gaagtttatc
    11881 tagcagaatg tttagcaaat ttggagcaaa aaggaattta tcttacctaa tataactaga
    11941 aaagccttct atctcctctt acttcattaa aaagctttaa ccatcttaga tcaagctaaa
    12001 atgtcgtaag ttttagggtt atcttcatcc tcacgtgcaa aactaaaagc gtactctagt
    12061 tttggttcat cgccaacttc cctagacata aatgaacatc tcctgacgtg gtttaaaagt
    12121 accgcatctt cgacgtctgt atacgatgca cagcacagat tgcttctttt gatctttctt
    12181 ttgatttttc tgttgcttct tctacacctt cttcgattac tacatcgatg cttacaattg
    12241 cttctgccta atattcccat gcatcgtcga tttaagcctc tgtagtcact gtgatcgttt
    12301 ccacatttta actcctctgt cgtggcttca gttgattttg tgatcagttc agttgctaag
    12361 ccagagtcag aacaacctgc cattgctagc gttaatattg atagtaaaaa tagttttttt
    12421 atcataaggt tccccccctg aaaatagact aaaataataa cgctttcttt ttttgttttc
    12481 agacgaaaat taataatttc taaatattct ttaaaaaagc ttataactat tatgcctttt
    12541 ttaatatata acacagttat ttttaaaaat aagaatgcta aatcactcta caataacggc
    12601 gaaagcacaa aaaataaaaa aattattacg ttattaaaaa aacagatgaa agatcaggtt
    12661 ttattagatc tcattctttc atcacttgaa gggatttaat gaactacttt ttcctctttg
    12721 tcgtttttat atattcgtat tccttcaaac agtaggatag cctctttcaa tgcttgatat
    12781 ttgaactgtc cgttacttaa aaatttagat gtgccgccct cttcctagaa catattcttt
    12841 tttacttttt cattaattga cagaccaatc gaacgaaact gtaaatttcg ataggataat
    12901 gattggtcaa actcattcat tttcaagaca tctcgattca tattcaagat aggaatcttt
    12961 ttatcaacat cactttattc gtattttcct ctaccataaa agcttcacgc tccacaaaat
    13021 tgttttatac actactattc agcccattca acgaccaatg
//
LOCUS       DARBDV010000082        12802 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000082 DARBDV010000000
VERSION     DARBDV010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.083"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..577)
                     /locus_tag="SCM56_002244"
     CDS             complement(<1..577)
                     /locus_tag="SCM56_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG1110991.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFFK"
     gene            719..1651
                     /gene="pfkB"
                     /locus_tag="SCM56_002245"
     CDS             719..1651
                     /gene="pfkB"
                     /locus_tag="SCM56_002245"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEG1110992.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            1633..3096
                     /locus_tag="SCM56_002246"
     CDS             1633..3096
                     /locus_tag="SCM56_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1110993.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            3093..3551
                     /locus_tag="SCM56_002247"
     CDS             3093..3551
                     /locus_tag="SCM56_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1110994.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            3548..4522
                     /gene="lacD"
                     /locus_tag="SCM56_002248"
     CDS             3548..4522
                     /gene="lacD"
                     /locus_tag="SCM56_002248"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEG1110995.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            4963..5691
                     /locus_tag="SCM56_002249"
     CDS             4963..5691
                     /locus_tag="SCM56_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1110996.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            5709..6905
                     /locus_tag="SCM56_002250"
     CDS             5709..6905
                     /locus_tag="SCM56_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEG1110997.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            6898..7893
                     /gene="lacD"
                     /locus_tag="SCM56_002251"
     CDS             6898..7893
                     /gene="lacD"
                     /locus_tag="SCM56_002251"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEG1110998.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            7890..8831
                     /locus_tag="SCM56_002252"
     CDS             7890..8831
                     /locus_tag="SCM56_002252"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HEG1110999.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            9007..9486
                     /locus_tag="SCM56_002253"
                     /pseudo
     CDS             9007..9486
                     /locus_tag="SCM56_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            9499..10389
                     /locus_tag="SCM56_002254"
     CDS             9499..10389
                     /locus_tag="SCM56_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1111000.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            10376..11185
                     /locus_tag="SCM56_002255"
     CDS             10376..11185
                     /locus_tag="SCM56_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG1111001.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            11199..11600
                     /locus_tag="SCM56_002256"
     CDS             11199..11600
                     /locus_tag="SCM56_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEG1111002.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(11646..12596)
                     /gene="yidC"
                     /locus_tag="SCM56_002257"
     CDS             complement(11646..12596)
                     /gene="yidC"
                     /locus_tag="SCM56_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEG1111003.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
BASE COUNT         4116 a         2064 c         2791 g         3831 t
ORIGIN      
        1 atttaaaaaa ttgaaaaata aagacataaa tcttattttt gatgttttta tttgttttct
       61 caccgttcaa agaaacaaaa ataacgctgt ctttcttagt tagtttccga ctgatgcttt
      121 tgataatttc tggatcagta tgcatctgac aattttttcc cgttaattga agttttacca
      181 gcatttcttg gccaatcatt tctgaaaatc ctcgggcaaa gacaaaaacc cggtcagcaa
      241 atttgattct ttgtattgca tcttcaatgt tcccgatttc caacatattg attgtacgta
      301 ccacttcttg ttcatttttt agaatggctc ggcggattcc tttgtctact ttatctaaaa
      361 atgaaaaatt gatgttcttg ttttctcctt cttttagatg atgcttaaac gaagtaaacc
      421 cttcatagcc tttctttttc attgtccgca caatggtggc tgttgagaca tttgcttgtt
      481 cacttagttt cacgatggaa tagttaggga tctctaggat gaggtgttcg ataaagttcc
      541 agaggtactg ctctgtttca cttaatttgt gattcatatt ctttccttct ttcaccaaaa
      601 gttagttaaa aaaagcacga cgagtttata aatgtaaagg ttttctttgt gttgggacga
      661 ttaagaaaac attttctatt tcgcgtattt cataatagaa tcaaggaggt cgttggttat
      721 gtctatctac acatgcacga tgaatttggc aattgatcta tttattgaaa cagatgagat
      781 gcatcctttt atggtaaacc gcacaaaaga ggatgacatt caggcaaacg gaaaaggggt
      841 gaatgtgtca ctagtattaa agatgctaga tttcccaagt acggcattag gtttttctgc
      901 tgggttcaca ggaaagtata tagaagacta tttacagcag aaacaaatcc aaacagagtt
      961 tattgaagtg cctggtatga cgcgtatcaa tgtatttaca caagtcaatc aaacgggaga
     1021 agaatacaaa ttagtgaatc aaggacctga aataccgcag acagctgttc ataatttttt
     1081 aaatcaaata agagcattac aagcagggga ttatctttgt gtttctggga gtttaccaag
     1141 agggctgtct ccaaaggttt taatagaaat cagtcggatt tgtttgatta ttgatagtag
     1201 tgatcaagaa gtcatggatt gtttacccta tcgaccgttt ttgctgaaac caaatgagga
     1261 agaactttct tcttggttcg gacgaaaaat ggttacagat gaagattact ttgtttatgg
     1321 acaacgacta gtagagtcag gcgcagaaaa tgttttgtta tccctcggtg agaaaggagc
     1381 gattcttttt acgaaagaca gggtgattcg aggaaactcg ccaaaaggaa aagttgtcaa
     1441 tacagcatgt gcaggtgatg ccatgttagg cactttttta gcaggataca tgaatcgacg
     1501 atcgcttgat gaaacgttac gtaaaagcat agcagcaggc agctctactg cttttcgtaa
     1561 aggactgact gattttttgg atgtggagga attaagtaaa caaatcaaaa tcaaggaaga
     1621 gaggttcgaa agatgggaaa atatcaacta attgcagcaa ccggttgtcc gacaggaatt
     1681 gcgcacacct acatggcgca agaggcatta gagcaagcag ctaggaaaaa aggcatcacg
     1741 atcaaagtgg agacccatgg acaaattggg atcgagaatg aattaacggc tgaagaaatc
     1801 aaacaggcag atgctgtcat tattgccgca gacaaagatg tccattcaga aagatttgtg
     1861 ggaaaacgag tcattgatgt ttctgtcagt acaggaatca aggatgcaca acggctgatt
     1921 gacgaagcat tggcaggaaa aggtactgtc ttagcagata atgaagctgc tgatacttta
     1981 gaaccagaag cgataagtgg tgctaatttt ggtcgtagca tctataaaaa tttgatgaac
     2041 ggtgtctctc atatgttgcc tttcgtggta gctggcggag tattgattgc tatttcattt
     2101 gccgtttggg gaatctattc ttctgatcca gatcatgcac aatacaatgc tacagcggca
     2161 atggtaaaag cagttgacga tgcagcaatg ggaatgatgg ttccagtttt agcagcttat
     2221 attgcagaag ggatcgcgaa acgtccaggg ttagtcgttg gatttgttgg tggaatcgta
     2281 gccatgaatg gcggtactgg atttttagga ggtattttag ctgggttctt agcaggttat
     2341 tttatcttat ttttgcaaaa gctactgata attttaccta aacaactaga tggcttgaag
     2401 gcaatcgtct tatatccagt tattggtgta gcatttattg gcatcgtgat gtctttgctg
     2461 gcaggtccaa tgaaggctat aaatgaagga atgatgagct ttttagctgg atttgaatat
     2521 tcgaatccac ttgttcttgg cgtgattgtt ggctgtatgt gtgcgtttga tatgggagga
     2581 ccggttaata aggcggcata tgtaacagga acagctcttt tgacacaagg aaacacgact
     2641 tttatggctg gtgcgtcagc agcttgcatt gcaccaccgt tgattactgg ttttgctact
     2701 ttattgtttg gtaaatattt tgatactaat gatcgtaatg caggtttggt taattttatt
     2761 ttaggatcaa ctcatattac ggaaggagct attccgtttg ctgcgaaaga cccgatccga
     2821 gtattaccaa cgatgatgtt aggctcatca attgctgctg tcttgaccta tctgtttggt
     2881 gtccaagtac cagccccaca tggcggtttt ttagtgttac ctgtagtgac gggaaaaatc
     2941 caatgggtta cagcgattct tattggttcg atcgttggtg gagtgatact aggtttgatc
     3001 caaaagaaaa gagtaactga taaagacaag aagagggata ataaagcaac aaatcgtaca
     3061 gttatcgaag aaggaagagc aggaggttta tcatgacaat cattcatgaa gagaacattt
     3121 ttttacaaca gccattagat aatcaagaag aagcatttaa gtttttagca aagcaaacat
     3181 tctgtttagg aatcgctcat gacgaaaaag aagtatatca aaaacttctg gaaagagaaa
     3241 aagaaggtac tacaggaata atgtcaggtt ttgctatccc tcatgcgaag tctcaaacaa
     3301 tcaatgaacc tagtttgatt atcgtcacac tgaaaaaagg aattgaatgg aacagtttag
     3361 atagacagtc agttgatttt atttttgcgt tatttatccc agattcagaa gcaggaacga
     3421 ctcatttgaa attattgtct ctcgttgcac gatcattgat gcgaaaagag gttacagaac
     3481 agttgagagc tgcaaaatca aaaatagaaa tagctacaat attaactaaa gaaataggtg
     3541 aagatatatg aagaaaatga gtatagaaaa acaggaacgt ttgaagcgat tgacggatga
     3601 agctggaatt attagcgcgc tagctatcga ccaacgtggt gctttgaaga aaatgatcaa
     3661 ccaatataaa gaagctacat cagaagatat catccaattt aaacagatgg tttctcagta
     3721 cctgactccg tatgcttccg ctattttact cgatccagaa tatggtctgc ctgcagcagc
     3781 gcttcgagag aaagaatgtg gtttgttgct agcttatgaa aaaaccggct atgatgcgaa
     3841 aatgccaggc cgactaccag atttactgaa tagttggtca gtgaaaaggt taaaagaagt
     3901 gggtgcggat gcttgtaaat ttttattgta ctatgacgtt gatgaaccag tagagattaa
     3961 tgtgcaaaaa caaatagtta tagaacggat tggttcagaa tgcgaagcag aaggcatacc
     4021 tttcttttta gagctccttt cttacgatgc aactattggc gatccgtctt ctcttgacta
     4081 tgcaaaagta aaaccgcata aagtgattga agccatgcga gaattttcaa aagaacgtta
     4141 tcaagtggat gtattgaaag tagaagtacc agtgaatatg agatttgtgg aaggatatgg
     4201 gcaagatact tgttatagtc aagcagaggc atccgcgtat ttctatgaac aatcgaaagt
     4261 gactgaacaa ccgtttattt ttttaagtgc gggagtcagc gctgcactat ttcaagatac
     4321 attgcgtttt gcaaaacaat caggttcgac attcaatggt gtcttatgtg ggcgagcaac
     4381 atgggcgaag ggagtagcac cttttgttac cgaaggcgaa gaaaaagcga gtagttggat
     4441 gttagaagaa ggaaaacaaa atattaaaga attaagtaca ctattagaag aaacagcaac
     4501 ttctatttat gttaacaagt aagagaaagg attgtctgag tgacagattc aaaaaataat
     4561 tagttattta aaaaaatgtt tgtcaaattt tatagaaaaa aagtataaaa ctgcaaaagt
     4621 tatctaaaac taataataac atttgtcatt aataagctat ttttagttgt tgtaaatgat
     4681 attggaagta ataaaacgat agttgtagga atatcatagg gattaatgtg gatcacgctg
     4741 ctcgataagt tagaccattt tgacggatcg cttatgtggt tcgtcttttt ttctgttctt
     4801 ttattggtaa ctggtatttt tgaggtatct ttgtctattc aagatataaa gctttaaaga
     4861 gctgttataa tagctttttt ggtctaaaaa acctttgaca aatgattaat aattcactat
     4921 tcttgtgttg tggttataac cagtaaagaa gaggtgattt ttatggagag ctcaaataaa
     4981 ggaaaatcat tgtattatca actagttgat ttattgaagg atcgtatcga aacacagatg
     5041 actccgcatg ataagctgtt atctgaaaga gaactttgtc aacactatgg ggtgagtcgt
     5101 accaccgtac gtctggcttt acaggaattg gaaaatcaag gttatattta ccgaagacat
     5161 ggaaaaggta cctatgtctc tgatttgtct gataagtcat cgaacttagc tggagcatat
     5221 agctttacag aagaaatgcg cgaaataggg cgcgtcccta agacggtcat tttagaattt
     5281 agacaaatgg aagctaataa gagaattgct aagaatttga acatccattt aggtgaaact
     5341 gtatttaaac ttaaaagatt gcgtttggca gatgaagagc cattgatggt tgagcgttct
     5401 tatctaccgg cgaaactatt tatgggactt gatttgccgt tacttgaaag taaaccgttg
     5461 tatgatgtgt tttcacagga ttatgaacaa caagttcaat tagctgatga agagttttat
     5521 gcaagtatcg tgcaaaaaaa agatgctgtt gtgctacgaa tcgctgatgg cgctcccgtc
     5581 ttacatctga cgaggataac ctataacgtg aaaaatgaga ttatcgaata tacagatagt
     5641 gtagcaagag cggaccagtt tcattataaa attcggcata tccgaaaatg attgagaggg
     5701 agaacaacat gtttgagcta tcgaagaaca aattaaaaca aataggtgca gcaatcacga
     5761 cacaagaaat aaaacaacaa ccagatctct ggaaagaaac aatcgataga ttgatgcaaa
     5821 gccaagaaaa tttagctcta ttttttgatc aaataaaaaa agaagcaaac ggaagaagaa
     5881 tacgtgttat ttttacgggt gcaggaacgt ctcaatatat tggggatacg ctcttaccct
     5941 atctaaatga acatggagac aatcagtttt ttatcttcca cagttttggc acgacagata
     6001 ttgtagcttc accaaaagaa tttttgtttc caaatgaacc gactttatta gtttcatttg
     6061 ctcgcagtgg caacagtcca gaaagtgtag cggctgtgca aattgcaaat gaacaagttg
     6121 aaacattgtt tcatttgacg attacttgtg ctaaagaggg gatgttggca aaaaacgcac
     6181 aggatgataa acagaattat ctgttattga tgccagagag agcaaacgat gcaggttttg
     6241 ctatgacagg aagttttacg tgtatgttat tgagtgcttt atggatcttt gatcaaacag
     6301 caatggagaa aaaagaaaga tatcttgaac aattagttaa aatcggtcaa gatcttcttt
     6361 caagagatca agaaattcaa aagttattac ctgatttttt tgaacggatc gcttatctgg
     6421 gttcaggaag tttggcaggt gcgacaagag aagcacaatt aaaaatttta gaattaacag
     6481 caggtaaagt tgctacaatt tttgattctt cgatggggtt tcgtcatgga ccaaagtctt
     6541 ttttaaatag cgagacactc gttgtaggtt ttgtgagcaa tcaaccatat acgcgaaatt
     6601 atgacttaga tattttagag gaaataaaag aagatcgggt cactgccaat gtcattgcga
     6661 ttgcgcaacc gggtactatt gatttctcag gtatgactgt gctattggaa gactcggtga
     6721 gtaaattgcc cgatgcctat cttgcattag cgatgattat gactgcacaa atcattgcac
     6781 tctatacttc aatcaaagta gctaataaac cagatacacc atctccaaca ggcacggtca
     6841 atcgagtagt caagggcgtc attattcata agtatgaaac cgaaggagaa atgacagatg
     6901 aatgaagaaa aaaatagggc aatggagaga ctatcaacaa aagatgggat tattagtgca
     6961 ttagcgatcg atcagcgtgg tgctctaaaa aaaatgatca aatcgttggg aaaagagcca
     7021 agtgaagacc aaatcagtga attcaaagaa ctagtatcag ctgagctgac accctatgct
     7081 tctgctattt tattggatcc ggaatatgga ttgccagcag caaaagtcag agcaaaagag
     7141 acggggttat tgttagctta tgagaaaacc ggctatgacg caactacgcc tggtcgtcta
     7201 cctgatttat tagcggattg gtcttctctc agattgaaag aacagcaagc ggatgcagta
     7261 aaattcttac tttactacga tgttgatgaa gcacccgaaa tcaatcattt gaagcatgtg
     7321 ttcatcgaac gtttaggtag tgagtgcgaa ggagaaaatc gtcccttcta tttggagtta
     7381 gtttcttatg atgcacaaat aagcgatagt tcttctccag agtatgcgaa agtcaagccc
     7441 cacaaagtta ttgagatgat gaaagaattt tctgatccaa aatataaagt tgatgtgttg
     7501 aaagtagaag taccagtcaa tatgaattat gtgaatggct attccacagg tgaatttatt
     7561 cacagcaaag aagaggctgc tgccttcttt aaggagcaaa gtgaagcgac tccattacca
     7621 ttcatttttt tgagtggggg tgtttcaaca gaattatttc aacaaacact gatttttgct
     7681 catgaatcgg gttccacctt caatggtgta ttgtgtggac gtgcgacatg gaaagatggg
     7741 gtcaaagcat acgtcgaaaa tggaaaagac ggagctattc attggttaag aacaaatggg
     7801 agagaaaaca tcgagacttt aaataaagta ataaatgaaa ccgctacccc ttggttcaat
     7861 aaaacaaaaa gcaaaaaggt gaagatgaaa tgattacgac agttaccttg aatccttcca
     7921 tcgatatcgc gtatacaatt gaagaattaa acctcgacaa agtcaatcga gttgaaacag
     7981 tcaataaaac agcgggagga aaaggagtga atgtggcgag ggttctaaat ttattaggag
     8041 aagaggtgat agctactggt ttaataggtg gccagcacgg tgaatatcta aagaaccagc
     8101 tagatcagtt agggatcatg catcagtttg ttccaattaa aggagagaca agggattcta
     8161 ttgctatcct gcatagttcg agacaaacag agcttttgga acgaggcccc ttcataacaa
     8221 agaaagaaca acagacattt accgatcatt tcgagcagct cctcgaaata tcagatatta
     8281 tgacgatctc aggtagttta ccccaaggat gccctcttga ttactataag caattggttg
     8341 ctttagctga taaaaaaggg aaagacatat tactagatac ttctggtaaa tttttgcttg
     8401 aagtattgga aggtaaggag aagccactac ttatcaagcc caatctggaa gaattaggag
     8461 aactgattgg ccaacgtatc tgttcttcga ttcaattaga aaaaattcag tccttattag
     8521 ctcatccaat atttgaagga gtgccatggg ttgttgtttc tttaggagag agaggtgctg
     8581 tggcaaaagt tgctcagtgt ttttacgaag tcattattcc taaaatatca gttgttaatc
     8641 ctgttggttc aggtgacgcg acccttgcag gatcggccca tgcgttatca aaaaggcaat
     8701 caatcgaaga gattctcgca ttttctatgg cttgtggtat ggcaaacgcg caacaagaga
     8761 aaactggctg gatcgaccct atgaaagtta cgatgttgat gaaacacatt caagttaaga
     8821 aaatcaaatg aacttaaata taaaagaaag cgttttcttt agttgggaag gaatttgttc
     8881 ttagcttaaa aatgaaatat aagaaagaaa aaatgaaaac gttatctttg aaacagaagg
     8941 gatatttgat gatgtcacag ttttaattga acaataaata gaaaaaatag ataaggagaa
     9001 tgcatgatgg caattattgg tgtaagaatc gatggacgtt tgatacatgg ataagtagca
     9061 aatctttgga caacgaaact gaatattagt cgaatcatgg tcgtggatga tgaggtagcc
     9121 acaaacacaa ttgaaaaaag tggattgaag ttagcgactc cagcaggggt aaaattaagc
     9181 attttgccaa tcgaaaaagc agcaacaaat attttagctg gcaaatatga ctctcaacga
     9241 gtattgatcg tagcaaaaaa gccagatcga atcttatctt tagtagaaaa aggagttcct
     9301 atcaaagaag taaatgtggg gaatatgtca caaacgaatg aaacacggtc cattacaaaa
     9361 tcaatcaatg tggtagatgc agatatcgaa gtatttaaaa aattagatca gcttggtata
     9421 catttaattg ctcaaatggt accaaacgat aaagcagaag actttatgaa tcttttaatc
     9481 aaataggagg aatgatccat ggatattcaa tggtggcaga tcttattatt aacattatat
     9541 gcaggttatc aaattttaga tgaattacag atctattctt caatgagtgc gccagtgttt
     9601 gcagggttat atgcaggttt agtaatgggt gatttaaacg caggtttgat tattggtggc
     9661 agtatgcagt tgacagtttt aggagtgggg acgtttggag gcgcatcaaa aatagatgcc
     9721 aactctggga cgattttagc aactgccttc tcgatttctc ttggaatgaa tccagaacaa
     9781 gcgattgctg ctattgcagt gcctgtagca agcttaatga tccaattgga tattttagct
     9841 agattcgcta acacatactt tgcccatcgt attgaccgtc acgtagaaga catgaattat
     9901 caagcaatcg aacgcaattt cttaatggga gctttacctt ggtcgctctc tcgtatgatt
     9961 cctgtatttt tagctttagc atttggtggt ggattagttg aaaatgttgt ttctgtttta
    10021 aacgggaatc taaaatggct aggcgatgga ctttcagtag caggagctgt tttaccagct
    10081 gttgggtttg ccattcttct gcgctattta ccagtaaaaa aacatctatc ttatcttatt
    10141 ttaggattta cgatcacggc attattagca acgttattct ctaatgttca attattagga
    10201 acaagtgtgg caagcgttgt taaagatctt ccggcaactt tcaattcgct gccgatgctt
    10261 gctgtagcat tgattggtct agcactagca atgatcagtt ataagaatga tcaaaacaaa
    10321 gaccatcaac cattagctag tgagccatca cattctgttg agggggaaat cgaagatgac
    10381 gaaatctaat tacaaattaa caaaagaaga ttttaagcaa atcaatcgaa gaagtttgtt
    10441 cacttttcaa ttaggttgga actatgagag aatgcagggt tctggctatt tatatactat
    10501 cttgccacaa ctaagaaaaa tatatggaga caatacacca gaattaaaag agatgatgaa
    10561 aacacatacg caatttttta atacttctaa tttttttaat acaatcatta ctgggattga
    10621 tcttgcaatg gaagaaaaag agggaattgc ttcaaaggaa accgtatcgg ggctgaaagc
    10681 tggattgatg ggttcctttg cagcgattgg agattccgta tttgctgctt tgattccaac
    10741 gatttttggg gctttggcgg cgaatatggc aactgatgga aatccgatag gtgtgtttat
    10801 atggattttt gctcagatcg ttgtcatggt ttttcgttgg aagcaacttg aatttgcata
    10861 caaagaagga atttccttag ttactactat gcaatctcgc ttaactgcat taacggacgc
    10921 agccacttta ttaggggtat tcatggtagg ggcattagtt gcgacaatga tcaatgttaa
    10981 attttcatgg gcaccaacga ttggcgatgt gacattgaat atgcaaaact atatcgatat
    11041 gatcttacct aaaattttac ctgctgggat cgtcggtggt gtttattggt tattaggaaa
    11101 gaaaaacatg actcctactc gagcgatttt tatcgtatta attgtgtgtg ttgctttatc
    11161 tgcattgggt gtgatttcga aataagggag agcgaaaaat gagtagacaa ttggtattga
    11221 ttagtcacgg aaaattttgt gaggaattga aaaaaagtac agaaatgatc atggggcctc
    11281 aagaggatat ctacacgtta cctttattac cagaagatgg cactgaaaca tacaaacaaa
    11341 agtttctcac tgtcacagat tcgttaaatg attttgtggt actatgcgat cttttaggtg
    11401 gtacacctgc aaatatcatt tctaaactaa taatggaagg acaacagatt actctttatg
    11461 ctgggatgaa tcttccgatg gtcattgaat ttatcaactc acaaatgatt ggttcagagc
    11521 ccgagtttgt tcgtgcagga tgtgaaagca ttgtctctgt caacgaatta attaatggaa
    11581 tagcagaaga ggaagagtaa caaaaaagat gagggcttag ctctcatctt ttttgtccta
    11641 tgtttttatt tccgcttctg ctgctttcct gcgttgcggc ctttgttgtt tgtactgttt
    11701 tttggttgtt tgatttgttt tgttgcttgt ttgttgttag ctggctttgc tgattctgtc
    11761 acgtcttttc ttggagtgac gacttgtttt ggcgggtttt gtttcaactc ttctgccact
    11821 tgtgctttca aacgtggttt catcataacg tttgtgatga atgtttgcag acagctgaac
    11881 acaccaccga ctacccagta aagtgtaaca ccggctggcg cactgatcga catgaagacg
    11941 atcatagcag gagagacgat tagcatcgta cgcatcgtct ttttctgttc ttcaggaata
    12001 ccgatcgttg agatgtatcc ttgaagcaga taggcgaccc ctgcaatgac aacaagtacc
    12061 atacttggtg agcctaaatc gatgccgtag aaagatgatt cccgaatacc ttctgtataa
    12121 cgtgccgtaa agtacaaggc agagaagatc ggcatttgga tcaatagagg cagacagccg
    12181 attccgccgg tcatactcaa tccgttttcg cggtatactt gctgcatttc tgcttgtgct
    12241 gccatttgtt cttcacgtgt tgttgcagct ttcaattttt gttgggctgc ttcaacttgt
    12301 ggttttaacg cttgcatttt ttcagattgg atcattgttt ttttcgattg actaatacct
    12361 agtggcaaga tgatgatacg gacgatcaca gtcattgcaa taactgccca gccataagac
    12421 caattgaaat tctcaactag atatgtaatc gcttggccca taggatggac gagtactcga
    12481 taaacgattc ccgaagtgtc agggtttccg tttgaatcca tccgcacaca gccagataaa
    12541 aagagcagga tactgacaag tccggatccc aataaccagt ttttccattt attcatagaa
    12601 tacttcctct tctataattt ttacacaaac ttaactatac taaaaaagcg catggaattc
    12661 aatcaacttc atagaaattt catataaata ttcgttcttt tacgtagcga aaaattttga
    12721 aaaaaagaca aaaaatcaag aaagaaatac ttggaaagaa aagagtgatt ccaagtgttt
    12781 ctttcttatt taaaaaacta aa
//
LOCUS       DARBDV010000083        12734 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000083 DARBDV010000000
VERSION     DARBDV010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.084"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="SCM56_002258"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="SCM56_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEG1111004.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="SCM56_002259"
     CDS             complement(322..891)
                     /locus_tag="SCM56_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEG1111005.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="SCM56_002260"
     CDS             1077..1979
                     /locus_tag="SCM56_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG1111006.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="SCM56_002261"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="SCM56_002261"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEG1111007.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="SCM56_002262"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="SCM56_002262"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEG1111008.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="SCM56_002263"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="SCM56_002263"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEG1111009.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="SCM56_002264"
     CDS             8547..8732
                     /locus_tag="SCM56_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HEG1111010.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="SCM56_002265"
     CDS             complement(8794..9990)
                     /locus_tag="SCM56_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEG1111011.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="SCM56_002266"
     CDS             complement(10160..11596)
                     /locus_tag="SCM56_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEG1111012.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="SCM56_002267"
     CDS             11889..12698
                     /locus_tag="SCM56_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG1111013.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2674 c         2249 g         4008 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcac gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gaca
//
LOCUS       DARBDV010000084        12200 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000084 DARBDV010000000
VERSION     DARBDV010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.085"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(82..639)
                     /gene="frr"
                     /locus_tag="SCM56_002268"
     CDS             complement(82..639)
                     /gene="frr"
                     /locus_tag="SCM56_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEG1111014.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(642..1364)
                     /locus_tag="SCM56_002269"
     CDS             complement(642..1364)
                     /locus_tag="SCM56_002269"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEG1111015.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1500..2381)
                     /locus_tag="SCM56_002270"
     CDS             complement(1500..2381)
                     /locus_tag="SCM56_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEG1111016.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2480..3262)
                     /gene="rpsB"
                     /locus_tag="SCM56_002271"
     CDS             complement(2480..3262)
                     /gene="rpsB"
                     /locus_tag="SCM56_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEG1111017.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3621..4100
                     /locus_tag="SCM56_002272"
     CDS             3621..4100
                     /locus_tag="SCM56_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HEG1111018.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4317..5408
                     /gene="phnW"
                     /locus_tag="SCM56_002273"
     CDS             4317..5408
                     /gene="phnW"
                     /locus_tag="SCM56_002273"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HEG1111019.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5401..5529
                     /locus_tag="SCM56_002274"
     CDS             5401..5529
                     /locus_tag="SCM56_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HEG1111020.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5533..6078
                     /locus_tag="SCM56_002275"
     CDS             5533..6078
                     /locus_tag="SCM56_002275"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HEG1111021.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6532..8223)
                     /locus_tag="SCM56_002276"
     CDS             complement(6532..8223)
                     /locus_tag="SCM56_002276"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEG1111022.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8643..9590)
                     /gene="arcC"
                     /locus_tag="SCM56_002277"
     CDS             complement(8643..9590)
                     /gene="arcC"
                     /locus_tag="SCM56_002277"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEG1111023.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9705..10724)
                     /gene="argF"
                     /locus_tag="SCM56_002278"
     CDS             complement(9705..10724)
                     /gene="argF"
                     /locus_tag="SCM56_002278"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEG1111024.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10815..12044)
                     /gene="arcA"
                     /locus_tag="SCM56_002279"
     CDS             complement(10815..12044)
                     /gene="arcA"
                     /locus_tag="SCM56_002279"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEG1111025.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3341 a         2528 c         2203 g         4128 t
ORIGIN      
        1 tttttttaat ttttaaataa aagaaatata aataaaaaca gaggaagcgg aatgatccgc
       61 ccctctgttt ttatgatttt attaaacttc caataattct ttttctttat cagcagcgat
      121 cttatctact tctttgatgc tgttatctgt caatgtttgt acgtcttttt ctaatccgcg
      181 caaatcatct tcggtaatat caccattttt ttgttgtttt ttatattcat cgatcgcatc
      241 acgacggatg ttacgaatcg cgatttttgc attttctgct tcttttttaa catctttagc
      301 taattctttt ctgcgttctt ctgtcagttg cgggataact aagcgaatga catttccatc
      361 attggttgga ctgattccga tatcacttgc cataatagct ttttcaacat cttgaagaat
      421 gctcttgtca aaaggtgtga tcattaaaac acgtgcttct ggaatttgga ttgatgccat
      481 ttggttcaat ggtgttggcg caccgtagta gttcacagta atacggtcca aaaggctagc
      541 gttcgcacgg cctgcgcgga tttgcccaag ttcacgttga agattttgtg ctgctttttt
      601 cattttttct tttgcttctg tcataattgc atctgccatt tttatttccc ccttacagtt
      661 gttccgatat tttcgcctaa gattgcacgg cgaatatttc cgtgttcatt taaattgaag
      721 acaagcagtg gaatatcatt atccatgctt agtgaacttg cagttgaatc catgacttgt
      781 aatcctttgg caataacttc cagatgagtc agttcttcaa attttactgc atttgcatct
      841 actttcggat cagcagaata aacaccgtct acattgtttt tagccatcag gataacatct
      901 gcgccgattt ctgccgcgcg taatgctgct gttgtgtctg tcgagaagta cgggttgccg
      961 gtaccgcctg caaagataac gatccgtcct ttttctaaat gacgttctgc tttacggcga
     1021 atatatggct ctgcgatttg acgcatttca atcgaagttt gcacgcgtgt cggtacaccc
     1081 acgttttcca acgtatcctg taaagctaag gcattcataa ctgtagctaa catacccatg
     1141 taatcagctt gagcacgttc catgcccatt tgtgctccga tttgtccgcg ccagatattt
     1201 ccgccgccca caacgattgc catttcaacg cccagctcgt gaacttcttt gatttcttgg
     1261 atgatttctt tgattactgg tggtttgatt ccaaatcctg cgtccccagc taaagcttct
     1321 ccactcaatt ttaagactac acgctgatat ttcggtgtga ccatgttcat tcctccaata
     1381 ttttctattg ccattttacc atacttagat gatttcgaca ctctaagact ggtttttctt
     1441 gttttgctta aaaaaaggga gcgcacaaac cgtgcgttcc cgtctgttat tcaacagaat
     1501 tattttttca cttggctcat aacttcttct acaaagttat cttcgcgttt ttcgatacct
     1561 tcgccaactt cgaaacgtac gaatgtttta acagttgcgc cttttgatgc aacgtatttt
     1621 tcaacagtca tatcaggatc tttaacgaat ggttggtcca ccaaagcgat ttctgctttg
     1681 aatttcttca agcgtccttc taccattttt tcaacgatat tcgctggttt gccttcgttc
     1741 aatgcttgtt ctgtaagaac agttttttca tgttctaatt cagcttctgg aatttgtgat
     1801 tcgtttacat aacgaggatt gatagctgct acgtgcatag ctacgtctct tgcaactgtt
     1861 tcgtcagttg ttccatctag aacagtcaat acggcaatac gtccgcccat atgcaagtat
     1921 ccaccaaatg cagcattgtc gtctttttct acaacttcaa agcgacggaa gctgattttt
     1981 tctccgataa cttgagttgc ttcgatcaag tcagattcaa ttgtaccttt atctgttttg
     2041 attttcattg cagcttccat gtctgctggt ttgttttcag caacaagttc tgcaatttct
     2101 tttactaaat cttggaacat ttcgttttta gaaacaaagt ctgtttctga gtttacttca
     2161 acgattgctg cagtgttacc tttcacagca actgaagcca atccttcagc tgcaatgcgg
     2221 tcatttttct tcgcagcctt tgccattcct ttttcacgta aaagatcaac tgctttttcc
     2281 atgtcgcctt ctacttcaac taatgctttt ttcgcatcca tcattcctac gccagtcatt
     2341 tcgcgtagtt cttttaccat tttagctgta acgtctgcca tgtttaactc ctcctgtgtg
     2401 tttttctttt taaaaaaagc tgcttcaaag gagagtgagg cttgtcgccc agcctttgaa
     2461 acagctccat cattcaaaat tattctgctg aagaattgtc gccttcaaca acatctacga
     2521 tttcttcgat tgatgttgct gtgttttctt ctgtgaaatc ttcttcaact acttgatctt
     2581 caccttggtt tccttcgatg aaagcatcag ccatttttga agtaatcaat ttaacggcac
     2641 gaattgcatc gtcgtttgaa gggattacta cgtcgatttc atctggatcg cagtttgtat
     2701 caaccatagc tacgattggg atatttaatt tgtgtgcttc ttgaacagca atacgttctt
     2761 tacgtgggtc aacgatgtac attacatctg ggattcttgg catatcagcg ataccgccta
     2821 agaatttttc aagacgttca cgttctttgt tcaaaccagc aacttctttt ttaggtagaa
     2881 cttcaaaagt tccatcttct tccattgcat tgatttgttt caaacggctg atacgttttt
     2941 ggatagtatc ccagtttgtc aatgttccgc ctaaccaacg atggttaacg aagtattgtc
     3001 ctgcacgagt tgcttcttct ttgattgctt cttgtgcttg tttttttgta cctacgaata
     3061 atgctacgcc gccttcttca gcaacgctct tcatgtaatc ataagctgca tctactaatt
     3121 taactgtttt ttgcaagtca atgatgtaga ttccgtttct ttctgtgaag atatatttct
     3181 tcatttttgg attccagcga cgagtttggt gaccaaagtg tacgccggct tctagtaatt
     3241 gtttcataga aattactgcc atgtttgttt cctccgattt ggttttattt ttcctcttct
     3301 tatcttctac cttgccggca aactttttca agcacctacc gacaatccaa taagaatgtg
     3361 gatttgctga tgcataaagc accctcagct attatacaga caaaagatcc gtatcgcaag
     3421 agcaaatata cttttctgct caacattcaa tagtttacct gaaagataaa aagaattcaa
     3481 gaaaaacagt agatttctct ttgtttcttt attatccttt ttccataaaa taattttagg
     3541 tataaaagac gaatattatt gggcaatcct tctttttttt gttatgatac atgtgaaatc
     3601 tttcgtaaag gagaaagaaa atgcgcaaag ctcaaagaca tttgctgatt aaacaagtca
     3661 ttgaagaatt cccgatccgt acgcaagaag aactattaaa caaacttggg cattatggcg
     3721 ttaccgccac gcaggcaacg atttctcgag atatccgtga tctaaaaatc gtgaaggcac
     3781 ctgatgaaaa tggcgtttcc cgcttcgtat tgtttcgcga aaaaggaact cctgagacta
     3841 ggactgagga agaggatcgg ctggttcaaa tgattgaaga cattgtattg aaagtcgaac
     3901 gagtccactt tttgacgatc gtcaatacct tgccagacaa tgctcattta tttgcatctg
     3961 ttttagatga gatcaaacct ccctctattg tcagtacaat tgctggattc gatacgatca
     4021 tggtcatctc ggaaaatgag gaggacgcaa aacgagtaga agaatttctt caccatccaa
     4081 ctgaagtatc acttttttaa tcagagcacg tattcctaat atggaatgcg tgttttttta
     4141 ttttcaatat tttccttatt gaataaacct ttacactctt tacaaaaata catcattttt
     4201 tattcaaatt tttcaacacc ttgattgaat cacaataatc atcatttata ttggatgtta
     4261 acaagagcag cttcttgctc tttgtgtatt tcaaaaaaga aatgaaagga aatcatatgt
     4321 ataaattatt aacttctgga ccattgacga cttctgaaac agtgaaaaat gctatgctga
     4381 tcgatcattg tacttgggat caagaataca aagaaattac acaatggata agaaaaaaat
     4441 tattggatct tgctcaagta tcggaaggaa tatacacaac ggtcttaatg caaggtagca
     4501 gctccttcgg ggtagaatcg gtattaagtt ctgtgatcag gccagaagat actgtactga
     4561 tttgttcaaa cggcgcatat agacaacgaa tgatcaacat gtgcgaatgt ctgaaactga
     4621 actataccgt ttatgaggta tcggaacatc aaatcctcca agctcatgtg attacacagc
     4681 tgattgaaga gaatccgtct atctccgcac tagcaatggt ccatagcgag actacttccg
     4741 gtattttgaa taatttggaa gagattgctg ctgtcacaca agaaagagat cttttgttta
     4801 tagtggacgc gatgtcaagc tttggtggga taccgatttg tgtagccgaa ctagggattg
     4861 attttttgat ttccagctca aacaaatgta ttcaaggtgt tccaggcttt tcttttgtac
     4921 ttgctaataa agcggaactg gaaaaacgaa aaaatgttag ccgaagtttg tcacttgatc
     4981 tatatgacca gtaccgcgtg atggaaaacg agaatggcaa atggcgtttc acttccccta
     5041 cccatacggt tttggctttt caacaagcgc tgaaggaact agaggcagaa ggaggaatcg
     5101 aaactcgata ttcacgttat ctcgccaata atcatgtgct acgacaaaaa atgacagaga
     5161 tggggttcca gcctttcatt caagaatatc aggggccatt catcacctct tttctttacc
     5221 ctaaggactg gcgatttgat tttaatcatt tttatcagta tttaaagcaa aatggatttg
     5281 tgatctatcc cgggaaaatc agcgatcatg aatgttttcg tatcggaaat attggcgaga
     5341 tctatccgga agatattttg gaactgacag caattatcaa agactataag gagaacgtta
     5401 atgattaaaa ctgtaatttt tgattgggca ggaacaactg tggactttgg ctgtatggct
     5461 cctgtccacg cttttagaaa tgcttttcta gaaaaaggaa tccaactgac tgacaaagaa
     5521 atccgctaac cgatgggaaa attaaaatgg gatcatatcc aacagcttct ttccctccct
     5581 tctgtcaaaa aacaatggat agctatttat ggacaactac ctcaggaaaa agaggtggag
     5641 gaattgtatc aacaatttga acattatctg tttaaagatt tggtcaagca ttctaccttg
     5701 aaacccgaca cattggaaac gatcgaacgg ttgagaaacg ctgggatcaa tattggttcc
     5761 acgaatggat ttaatgctcc tatgatgaca atcgtagcag aaaccgcaaa aaaagaaagg
     5821 tattcaccag acttcatggc aacttttgaa gacgtcgaag ggtatggtcg accgtttcct
     5881 tatatgatcc ataaaaacat gcagtacttc aaaaacaagt cggttgatga agtaatcaaa
     5941 gtcggagata cggtctcaga tatccaggaa gggaaaaatg ccggagtgtt gacagtaggt
     6001 gtaattgaag gtagttcgct aatggggatt tcttatgatg agtaccaaaa attagagatg
     6061 gggaaaaaga tcgtttaaaa cagtctgttg ctcttagatt ccaacaagcc ggcgctgata
     6121 tttgtgtaaa caatttaaaa gagttaacag aatacatttt gtacgctgac atgaatccag
     6181 aatgactttt atacaaaata agaggttgtg aaaaagctgt cctgtatcaa gtaataaaaa
     6241 cagccaagca aaaatagctc ctcatatttt tcgtttggtt aggcttatta ccgcagatgc
     6301 aagcttttga acaccgttta ttcgaaaaaa aaaagggagc tgtgacacga cttttgtcac
     6361 agtcccctta tttgcaaaca aacagtgttt aagagacaac ctctccacta aacggtgttc
     6421 attcagctga ctctcggtat ttgctcaaaa aacgataaaa tttgaaaagc tattttctct
     6481 tttgtttctc taatactcgg atcagaacgc tgatttcaca ttctcttttt cttacatctt
     6541 atctggtgca tgaagtccta gcaaacgaag gtcttctttc aataatacag tcactgcata
     6601 aactagagct aagcgtgctt ctttttgttc atcatcagct aaaatcttcg tatgagcata
     6661 gtatttgttg aatgcttgtg ctaatttgat tgcatgtttc gcaatcactg acggttcgta
     6721 tttttcccca gcagacaaga ccgtttcagg gtatttttga actaatttaa ccacttccca
     6781 gctggtatcg tcattcaatg catagtctgc tgtttctgaa gggacaaatc ctgctttttc
     6841 caacaggctg actgcacgag catgtgtata ttgcacataa ggtccagttt ctccttcaaa
     6901 tcgaacaact tcttccaggt taaagtcaaa tgtattcagt cgatcgtttt tcaaatcgtg
     6961 gaaaatgact gccccaaccc caacttgttt agctacagca tctttgtttt ccaaatcagg
     7021 gtttttctcg ctgatttgtt ctttagcaga ttggattgct tcatttaata cttcttctaa
     7081 taagacgatc tttcctttac gagttgataa ttttttacca ccttgcgtga tcaaaccaaa
     7141 tggaatatgg tgcatgtcgt cagaccagtc aaagcccatt tccttcaaga cagctttcaa
     7201 ctgtttaaaa tgatagcttt gttcattccc tactacatat agagattgtt tgaagtcgta
     7261 agtacgttta cggtacaatg cagcagctaa gtcacgtgta atgtatagtg tcgcaccgtc
     7321 tgacttctta atcaacgctg gattcaggtc ataagcagat aggtcaacga tctctgcacc
     7381 tttatcctcg ttcagcaaat gtttttcttc taaaagtttg acgatttcat ccatcttgtc
     7441 attgtagaaa gcttcaccgt tcaacgaatc gaaacgtact tccagcagat catagatttt
     7501 attgaattct ttcatcgatt catcacggaa ccattgccat aattgaatag cctctttatc
     7561 gccttcttcc agacgtttga accaagcacg agcttcttca tttaattctg gttctgtttc
     7621 agctacttca tggaattgga cgtataaacg aagcaattca ttgattggtt ctgctttaac
     7681 agcttcttct gtaccccatt ttttgtaagc gacgatcagt tttccaaact gtgttcccca
     7741 gtcacctaaa tggttgatgc ggattggctg ataaccgatt ttttccataa tgaaaccgat
     7801 cgaattaccg ataactgtcg aacgcaaatg tcccattgag attggtttgg caatattagg
     7861 agaagacatg tcgattggta ctgttccttg attcccgatg ttgctatccc cataatgttc
     7921 tttttctttc actactgttt gcaaaacttt tttgctgata agttctttat tcatgaaaaa
     7981 gttcaagtaa ggtccgacaa cttcgatttt ttcaaagttg gcagaatcga ttttttctgc
     8041 taaatctgca gcgatttgct gtggcgcttt tcggtaaact tttgctaatg aaaaggctgg
     8101 aaatgcaaca tctccgtgtt cagctgattt tggattttct agtaattgct cgacttgttc
     8161 cagtgttaaa tcatctttga cagcgtcata gacagctttt gcgacaagat ctttattatt
     8221 catctttatt cctcctaaag tgttcccttg attgttaaac aaaaaaaatc tctagcgacg
     8281 gcatacttag tatgccatca ctagagacga gatttcccgc ggtaccactc caattgtcct
     8341 aaaaaaggac cagctccatc tgatatcggt agacgaccgt ctgtttaaaa cagaattctc
     8401 caagtgcgct tcgttgtatc ttcttcattc ggcttccacc acttccgaat tcgctagcca
     8461 agaatgatac aagtactctc ttgttcaaca aatcttagtc ttattaaact ttcttgaatt
     8521 atatcagaaa aaaaaacgaa aggctagtcg tttcataaaa aaacaatcca gatggcctaa
     8581 aggagaagaa ataccacctg gatagctata aatcatttcg taaatacgaa agacttaaca
     8641 aattaatcag ctacgataat cgttccttct tcagaagcta aaagattctc gatattttct
     8701 aaagaagtga tgattgcctt agcgtttggt tttgcttcaa caaatgcaat agctgcttct
     8761 actttcggaa gcatgcttcc tggagcgaat tgcttttcat caatgtattg tttcatttct
     8821 gaaacagtaa cagtttctaa tttcttttga tctggttttt ggtaattgac atacacattg
     8881 tctactcctg tcaatacgat cagcaaatcc gcatcgatga tttcggctaa tttttctgaa
     8941 gcaaaatctt tatcgattac ggcttctcgt ccttctaatc cagtagctgt ttctacaaca
     9001 ggaatcccac cgccaccaac agagactgtg ataactcctt gatcgaccag cgtttcgatg
     9061 acacgtgctt ctttaatcga gatgggttta ggggaagcga ctacttttct ccatccacgt
     9121 ccagcatctt ctttgaatgt cacagtggag tcagcattca tttgttcttt ggcttcttct
     9181 tccgtgtaga atggaccgac tggtttactt ggatttttga atgaaggatc attttcatcc
     9241 acaataactt gagtgactaa agaaaccaca tctttatcga tccctttttc cttcagtact
     9301 tcgcccatgg cgttttgtaa ccagtaaccg atagatcctt ctgtcattgc aacacatgta
     9361 tctaaaggca ttgccggtgt tttctcagaa tcagcagctt gctgctgaat caataggtta
     9421 ccgacttgcg gaccatttcc gtgggagatg atcaattcat ctccttgttc gataaatttc
     9481 actaaatact ttgctgtttc cattaacgct tcttgttggg cttttgcact tgcatccgta
     9541 gacaagatgg cattgccccc taaagcgaca actacttttc gtttggccat ttcattcgtc
     9601 ctttcttcag ttctaccgaa ttttctagat aacaaaacga gccagcgttt tgtgattttc
     9661 cctcaatgct gactcgtttt ttcgtgagat gaatgtggtt atgcttaaac gcgagggata
     9721 aacaagttac ctaatgttgc tgccatgatc gctttgattg agtgcatacg attttctgct
     9781 tggtcaaatt gacgagcata tttgctgcgg aagacttcat ctgttacttc catttcagtg
     9841 atgccaaagc gttctttcat ttgttcaccg taaacagtgt ttgtatcatg gaaagctggc
     9901 aagcagtgca agaagatcaa acgatctgtg ttgtgtgttt tttctaccat ttccatattg
     9961 atttggtaag gtttcaacaa tttgatacgt tcttcgaatt tatcttcttc acccattgat
    10021 acccatacat cagaataaag gacgtctgct ccgtctacac ctttatcaac atcgtcggta
    10081 atcatcaatt gtgcaccgga tttttcagca aagccttctg ccattttgac gatttcttct
    10141 tcaggttgta attcttttgg tgcaacgata cgcatattcg ctcctaaaat tgcacctgtg
    10201 actaataaag agttagccat attgtttcga ccatctccgc agtaagcaac agtgatacct
    10261 tctactgttc caaagttttc ttggatagtt aaaaagtcag cgatcatttg tgttggatgc
    10321 cattcatcag tcaagccatt ccaaactgga acaccagaga attctgctag ttcctctacc
    10381 attttttgac tgaatccacg gaattcgatt ccgtcaaaca tacgtcctag tactttagct
    10441 gtatcttctg tggattcttt tttacctaat tggatatcat ttgcacctag atattcagga
    10501 tgtgcaccta gatcgatcgc tgcagtagta aatgcagaac gagtacgtgt agatgttttt
    10561 tcaaataaaa gcgcaatatt ttttccttct agatagtgat gtgggatacc acgttttttc
    10621 aagtctttca agtgttctga aaaatcgatc aaatattgta attcttcttt tgtaaagtct
    10681 ttttctgcta ataagcttct tccttggaat actgattctt tcatattaaa cattctcctt
    10741 tagattgttt ttatttaaga ttaaagagga aatcccgacg gcagctgaca gtaagactgc
    10801 cgtcaggaaa caaattactt caagtcttca cggacaagtg gttggctcat gcagcgtgga
    10861 ccaccacgac ctcgtgacaa ttcacttgaa ttgatttcta acactttgat gccatagcta
    10921 cgaagcaatt cgttcgatac ataatttcgg ttataagtga ctacaacacc tggagcgatt
    10981 gccaatgtgt ttgatccatc attccattgt tctcgaggtg caacgatctc atctccatta
    11041 ccagttggga tcagtacaag atcatctaat ccaagagcag ctttcatcgt ttcgtgtaag
    11101 ttatctgaat gagtaatctt gatatcatca ccatctggtt cgattgtaaa gacatccatc
    11161 ttaccgtctt tactttgaat tgctggatgg atagtgaatt tatcgtgatc taccattgta
    11221 aatacggtat ccaaatgcat cattgcacgg ttgttcggaa ttttgattgc caacactttt
    11281 tcaaatcctg aattttttgc gaataggtgg cgtgctaatt tttctaaagc tttcgcatta
    11341 gttcgttgag aaatcccaac agctactact ttatcgctta agatcaattc gtctccgcct
    11401 tcgatatgat ctggattttc acgatccaac catacttcga ttcctttatt tgcaaatctt
    11461 ggatgatgtt tcaaaatata ttctgtaaac atcgattcac gacgacgtgc ttcaaaagtc
    11521 atcttattga ctgtcatccc atttcccatt gatgcggaag ggtcgcgtgt aaagtacaag
    11581 tttggcattg ggtccatgta aaatggatag tcatcgtctg ctgacagatc atatagactg
    11641 ctagaacgta cttcgatatc ttttgtacga accccagcca tgattttatc taccatttct
    11701 tgcgtttcca ttgatagaag gaactcatga agaccctctc ttacagcatt cgatgcaata
    11761 tgagattcat ccaacatttt attcaagaat tgttctttga cattgcctgc gtcaatcgct
    11821 tctgccgcta atttttccaa gtaaagagtc tcgacgcctt cattttgcaa tgtcttcgca
    11881 aagttgtcat gttcttcttg agcaatcgga agatatggaa tatcatcgaa caacagacga
    11941 tccataatat ctggtgtcaa attttccact tcttgtccag gccgtttcag taaaactgtc
    12001 tttaatttcc ctatttcaga gaaaacgtga ataggtttat ccatgttgtg ttgcctcctt
    12061 ctttttgatt acatgtatag gatatcctcc ttttgtaatc actttcataa ttttgtctca
    12121 ttcaaaatga gacattcctc acattaatat tcgataatta aataattaaa cgtttttcta
    12181 tttttaagcg taatttattc
//
LOCUS       DARBDV010000085        11667 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000085 DARBDV010000000
VERSION     DARBDV010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.087"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(37..216)
                     /locus_tag="SCM56_002280"
     CDS             complement(37..216)
                     /locus_tag="SCM56_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111026.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(597..1304)
                     /locus_tag="SCM56_002281"
     CDS             complement(597..1304)
                     /locus_tag="SCM56_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111027.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(1297..1734)
                     /locus_tag="SCM56_002282"
     CDS             complement(1297..1734)
                     /locus_tag="SCM56_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111028.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(1749..2000)
                     /locus_tag="SCM56_002283"
     CDS             complement(1749..2000)
                     /locus_tag="SCM56_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111029.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(2000..2206)
                     /locus_tag="SCM56_002284"
     CDS             complement(2000..2206)
                     /locus_tag="SCM56_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HEG1111030.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(2643..2903)
                     /locus_tag="SCM56_002285"
     CDS             complement(2643..2903)
                     /locus_tag="SCM56_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111031.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            2978..3181
                     /locus_tag="SCM56_002286"
     CDS             2978..3181
                     /locus_tag="SCM56_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HEG1111032.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="SCM56_002287"
     CDS             complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="SCM56_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEG1111033.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(3810..4709)
                     /locus_tag="SCM56_002288"
     CDS             complement(3810..4709)
                     /locus_tag="SCM56_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEG1111034.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(4722..5315)
                     /locus_tag="SCM56_002289"
     CDS             complement(4722..5315)
                     /locus_tag="SCM56_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEG1111035.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(5391..5540)
                     /locus_tag="SCM56_002290"
     CDS             complement(5391..5540)
                     /locus_tag="SCM56_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111036.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            complement(5556..7760)
                     /locus_tag="SCM56_002291"
     CDS             complement(5556..7760)
                     /locus_tag="SCM56_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HEG1111037.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(7771..9231)
                     /locus_tag="SCM56_002292"
     CDS             complement(7771..9231)
                     /locus_tag="SCM56_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEG1111038.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            complement(9292..11664)
                     /locus_tag="SCM56_002293"
     CDS             complement(9292..11664)
                     /locus_tag="SCM56_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111039.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT         2892 a         2432 c         1957 g         4386 t
ORIGIN      
        1 tgctatcact tttagttttt aggataaaaa gtgacgtcac tttttgagtg ctggtacctc
       61 aaatcctgct ttttcttggg caatcaataa atctaacgca atacacataa tcctggattc
      121 acttttgccg tacttctgct ttagttgctg caattctttt tgtctctttt catccaacgt
      181 cacgggtaac cgtttatttc ctattgcaat tggcattagt actctctcct ttcatcgaaa
      241 taaccctatt aaaaatatgg ctgcaatcaa cttgaatatt agactcacaa gggttaacac
      301 aaagcgccaa aagatcaaac aaaataccag tattcctaag aatactacga agtttaaatg
      361 tagagctgca ccatgacgca taagtatctc gccaacaaac agtacgaaca atagaggaat
      421 gattttattc atatggatct ccttctttct cttaggttat cgggctttgc ccgcttattt
      481 cgcaccacct aagcggcggg ggcggttgga tcgattggtg cgaaataaga accattccct
      541 taatttcaag gttttttgtt cttatttcac acatcctcac ttttcggtat aaatggttat
      601 tgaccttttt tcctttcaaa tcgttgatat ggcattcgtt tacggtcaag ttcattttgg
      661 atttcttctg tatcgtcgta agtgtaggtg tctaaaaaat cagttaaagt cgtgtaccag
      721 tcgtcgcctg ggttatctac ttcttctaca tattccctca aaaatgcttc gggtacagtg
      781 agttcataat ccattcccat taacacgata gattccgtcc cacacgtcaa aaatctagta
      841 aatacaaggt aataatcgtc taccgtcgca tctttgctgt ttagctcttc catttcttct
      901 tccattaatt ccatgctctc aaaaattgaa taatattctg attctgagta atagtgtttc
      961 ctgcaaatcg tgtagctgac aaagttttcg accagctcac tcctttctag gtaaacattc
     1021 atgatatatt tttctgtttc tagtcgtaga agctccgctc ctttttttaa tgtgtcagga
     1081 cgacgtttat acacgtctaa acgattatgg ctgactgtaa ccttcgtttg actcgtttca
     1141 ctttccatat acaactgttc ttccactctt tgaataaagt acttcatttc ttccatacaa
     1201 ctaaagtctt tttgaatccg tctgattact tcattcaagt gttctttgtt tgttaaaaat
     1261 tgcgaaacat cttctgttgt tagctgataa accaccttat tcattatctt tctcctccga
     1321 ttcaatcgta taaaatgcac catttttttc gatattttca aaagtatcta gccagtcgct
     1381 agccattgct ttagctttct taatctgtgc gaattttgca taagtcagtt ctaaggcttg
     1441 atattcggaa ggtttgatag tgcttggttt gacacctact tccaagcata gtaaggcttg
     1501 gaaaaaccat tttccatttt gttttttcag ttcttccagt gttcgatagt acgtcgcaat
     1561 gagattcatt tcgacacctg attcaatcat ttcttccact gcgactgaca atttttcctt
     1621 gtaatgtttt ttgggtacac gtgtttcatg gtataggaac gtcatatcct ccattttttg
     1681 tgccatttta tccaaaggta atttgacaaa ttcgtcatag taattttgag ccatattttt
     1741 tctccttttt agttctctaa tttggttaac caatattcga tttcataagt ttccatgttt
     1801 tcttgataat gcagttcata tcctgcaatc ttcactaggt cttcaaatag catagtcggt
     1861 gcatcctcta ctaaaggggt ttctatcgtc cagtaccgcg gaataagtaa agcttctgct
     1921 actttttcag aaatgacttc tttgtccacc aaaatttgaa cgacttcttt tacagtcaat
     1981 aatttgtgtt gtgttttcat caccgtttct ttactttctc atcaatcaaa acataatcgc
     2041 cataagacaa ggtatatttt ttcccgcatg ttggacattg atacaaacga tagcttttga
     2101 tacatttcgc tctttctcca caatcgcatt cgacagtatg tggtgcatga tagtttggga
     2161 ataattcgct gaagggaaca ccccaataat caaatttctt tcgcatgatt gattctgcct
     2221 cctcttagga aaggttttat ttcctttcgt tagttctttc tttattgatt tgttggccag
     2281 aatgaatcaa cgaaaagcga caaggacaag ggcgcgcagc gtgccattta cccttgtcct
     2341 tgtcgctttt tggtgataaa aggcaacttg ttgccggtct ggcaaatcaa taaagagata
     2401 gcgaacagga acgaaccttt tggtgagagg aagttttttc tattttgtct tgctttgcaa
     2461 gacaccatat cttagtgaac gtgcaacgtt cacttccttt cttctcgaac ggcggtggcg
     2521 gttggagtga gagaaaggtt tttcaattga gaaggcactt gtgtcttcct aaaaaggtct
     2581 tttcctgttt ctatgtaaaa caaaaagagc tgcaatcctc atttaggact gcagctcttc
     2641 ttttatatgc ccatcgatcg cgtaaaatga tcggggtttc taatttgcat cgattcaatg
     2701 actaattgat tccgtttatt gaaatgacct gtgacggcaa tattgtattt tccatcatcc
     2761 ataattaaca atttatcgat aatttcaata tctgcaacta cacaattaat cgattcatga
     2821 attgtttgta aagtaaagcg aaccaataat ggttgtgttt ttattactcg gattttatca
     2881 agcaaaccaa catatttgtt catcacgatc acctcgctaa aattatagca aaaacagcta
     2941 aaattgagaa tttttattcg attttagata tgctataatg atcaagacag taaagtttgt
     3001 gacggtggct atccgattga aagtgaggtg gtgcgtatgt gcgtacttcc aaaatcttac
     3061 agaaaggaga agccttttgc ctgttgaaac ggcgctagga ttaatgatta gtttcggttc
     3121 tttgatcgca acaatcattt ttggaatctt agcagttgta aacaacaatc aaaaaaaata
     3181 accgtcagct ttagccggtc gacgattatt tttcaaaaat aatattaagc tggtcaccgt
     3241 cttaaacggt gctgttaggg gagttgttag cgcaactcct cttttcgtat ccattataac
     3301 aactttattg atcttttaca atcagaatgg taaatcatca tcactaatat cgattgattg
     3361 tccgccaaat ggattttgtg aagcgttcaa tccattgttg ttaggcgtag ctacatcccg
     3421 ttgttcaaag gtggtagggc ttggactgct ggacgattga tcagcgtgcg cacggctttc
     3481 tgtcgctgct ttactttcta atagttggaa attatcggcc acaacttctg tcacatacac
     3541 acgttgtcct tgttggttat cgtaggttcg ggtttgaatc cgtccaacaa cgccgatcaa
     3601 actgcccttt ttagctaatg tcgccatcgt ttctgctggt ttgcgccaaa tcacacagtt
     3661 aataaaatca gcttctcgat tgccgttttg attcgtgaaa tttcggttga cagctagggt
     3721 aaaagtcgct actgcagtac cactcgcggt ataacgtaaa tctggatctt tggttaagcg
     3781 tcctactaaa gttacattat tgatcatctt tatctctcca tttcttgatt catttgttgt
     3841 gatcttttaa actgccgttt tctttcttga ttttcaattt tgatcacact tttcattagg
     3901 tgatcaattt cgttgactgt atctgtaaat gaaatggaat tcgcaaacgt gttaactgag
     3961 cgatattttt cccactgctt ttccaaatag tcatttcctc tgatcttatc catttctctt
     4021 tcataataaa aggacaacgg ttttgtatcc ccacgttttg taaatgtatt tcgaacactt
     4081 ttgtataact ctttcgtatc gatattttct agtttcggaa tggtaaataa gtcgtagtaa
     4141 tcttttgctc tcgaattatc tgtgccaaag ctgaacgtag cacttagctt ttctgcaata
     4201 atttgttctg ttggataagc tggaatctcc acttttttat cttcaaacat taattggtga
     4261 tagtgtgtat ctacttcagg ataaatagaa tctcctgttg tcacgtccat tttaaacggt
     4321 acacgtactt tctctaaatt agccaccaat ttaatttgaa atccaggata aaaatcggct
     4381 tctcttgttt ccttcattcc ttgaatagag aaaacaatgc cttcttttgt gggttttcta
     4441 gttaaatcat taaaaatgct cttcaatttt tcttcagtta atttgctatt tctaaatgtc
     4501 gtatcgatat caattgttgt ccttctatcg attccatatt ttgatcctaa taaaaagccg
     4561 cctttcaaaa tgaagtcatt tttgtacttt gattcagaaa tttgttcaat tagacgatcc
     4621 atcataaaat gccgttgcac taactgtgca tttaatcctg tactctttgc taaatttctt
     4681 gtttttgctg caaattgtgt cggtgtcatc tttttcctcc atcaatataa gctttgcatg
     4741 tggtatttca tttctttttc tacatgtagt tttgtcatgt aatctcttaa tttacttgga
     4801 tctctcaatg ggtcttccat gtactctttt aatgcttcta atttcatttc atattcaaat
     4861 tcatatctcg gattccaaat atcacaaagt gtacgctctt tatcatacat agtaatagga
     4921 tttccttgaa aggaagttcc ctctactttt cctagttcat aaacttcttt cttcacgaaa
     4981 tgaaattgta actgctgctc ttttgtaatt gaacgatcaa cgttatagcc atggggaaaa
     5041 gttaagtgaa tgaatttcgg catattcgta gaaagctggt aaatatctaa agtcgtttca
     5101 tgagagaaga ttcccctgga aaatcgttgc tgaagccaaa ataaatcatc tattccatga
     5161 ccatcctcta cataataacc acgatcaact ttgatcaatt ttccttgatt caccaatctt
     5221 tgtacagtgg ttgctgcgat tccaaatttc ctggcttcct tcatagataa atttccatcg
     5281 cgaatagcca acaattcttt cagtttatct tgcatttact tcccctccct tcgttacaaa
     5341 tacactacat caaatatatc atagtagttt atttgtaaca agattaacgt ctatcgatct
     5401 aattcgattt ctttttgaat gcgctgctgg ttctttaatt gttgcagtct tcttttattt
     5461 gcacgtaatt tctcttgttc taaacgaatt tttgcttttt gattttcgat ttcttgttgt
     5521 ttgtgtgttg tatttcgcat aggataaccc cttcattatt ttgtagtgcg gcgtgttctt
     5581 ttcttatagt tcgttttttt cttttcagaa aattcaaagc ctgttgtaaa gtcttctttt
     5641 aaaacgatcg ttgcttcaaa cgaacctttc tgacctttta gtcctctgac tgattggctt
     5701 gttcggccgt tttgaatgat ctctttcagt tgtgtggctg tgagtttctt tttggcaatc
     5761 gttgtccata atttaaaatc acaggcttca gacgtacatg ccgccacatt ttgataaagc
     5821 aagacttctt gttcccgaca ttttggacaa acacctaaat gacgcgttgc ttcctgtttt
     5881 tcactggctg ctttcacctc ttgtaacgtt gatccaaaat ctgttgactg gatttgtccc
     5941 ggtacgactt ccaacaagtg agagacaaat ttttggatat tagctaagaa agtagcttgt
     6001 gttcctttct gctcaccaat ctttaataag taactttccc atttggcggt catttcggca
     6061 cttgttagca gcggctcttt ttccacggct tggcaaagta aaatcccttt atttgtcacc
     6121 acaagcttat ttttttctac cttgatatat tccttttgtt tcaaagtttc aataatattt
     6181 gctcgtgttg cttctgtacc gattccttct acttctttca atatggattg tgcttcttcg
     6241 ctatccacgg tttttccagc tgttttcatc gctgtgatca acgtaccttc ggtataaggt
     6301 tgtggtggct tcgtttcttt ttcaaggact tgtagatcta ccgtcaccgc ttctcctttt
     6361 gtcactttag gaagtagcgg ttcttcttcc ttttcttttc tgtcgctctc tttgccaaac
     6421 agctttttcc aacctagatc tactggtact tttccgactg tctttagctg aagctgctga
     6481 acttgcgtca agatcgttgt ttcttcatag gtatactttt ccgcaaacat ggccaccgtt
     6541 gtttttaaca cttgttcata aatagcctgc tgcagtggac ttaattgttc aaaagccgct
     6601 gccgtcatga ccgtctttgt aggaatgatc gcgtaatgtt cctggacttt cttattattc
     6661 acatatcgac tattcggtgt atgaatagga gtaggaatgg cttcggcctt taaaaagtgt
     6721 ttgtattcgt ctaagtgatc caacagatac gcatactccc cttctgtaat ataaggggta
     6781 tctgttcgtg ggtagcttag atatttccct tcatacaacc cttgcatggc ttccaaagta
     6841 tctttggcac tagctttcat gagctgattc attttagatt gaaggctgga tagagaaaaa
     6901 agacgtggac ttgccgcact ttttttctcg gtttcaactt ggtggatcgt acctggctgt
     6961 tttcccaatt gaaccccctt ttcttttaaa taatcttcaa atgcttcttg cgtcgcttgg
     7021 gtttcattcg gatcaagctt cgcatcaaat gcgccatttt gagcgattac ttgggctttt
     7081 ccttcaaaat atggttcttt ttggaagttt ctaatctttt cctgtaattg ataaatcatg
     7141 taaagcgttg gggtttggac tctgcctaag gaaaaacttc ctggtatgcc tttttgctgc
     7201 aagagtaaac tatacagcgg gctaccgttc atgccgatca accaatcggc gatctggcgt
     7261 gcttgtgctt cttcaaattt tgggtaggtc tcttctgcat ctcgtaactg ctggaatccc
     7321 tgataaatgg cttctttttc taaggagttg atccaaagtc ttttaaatat ctttccatga
     7381 tcggcgcctg cttgttgaat gatcgtccaa gctatcagtt ccccttcacg atcgctatct
     7441 gtcgcaataa taatggtgtc ggccgattga agttgctgtt tcaccacctt gaattgcttt
     7501 ttcttatctt taggaacatg atagtgataa tgtgtaggga aaataggcaa atgctctaat
     7561 gaccattgtt tccaattttc atcgtacata tcgggcgaat ctaactcgac taaatgacca
     7621 aaaccatacg tgatcacagt ttctcctgaa aacaatcgat cttggatctc aaaataccca
     7681 tcttttcggg tagctttaga aaagctatcg gcataagcct tcgcttgtga cggtttttct
     7741 gctaaaataa cggttttcat catgacgatt tcactccctt tcttgctgtc taccttgttg
     7801 aaacaacatg gcacgttttt gctttctgat ctcttgttgg tagatttcat gcttcaattg
     7861 atcattccaa tcctttttta cttcaggtac aaattcatgt tcatgatttt tatagccttt
     7921 gtagtattgt tctgctttat aagagaaaag aatctgagca ttaatatccc ctttttcttg
     7981 ataaaaacga tcaaaatcgt acgtaccatc cacgcgttca caagctttta attcttttgc
     8041 gacttctcta caaatatctt tcaaattaat agcttctatt gaatcgttaa cagaaagctg
     8101 ctgaccaaaa gacgcattta ctttgtgagc ggaaaccaat tggttcgtta attgcagatt
     8161 gttttctgtc ttgtgtatgg ccataatagc ttctggtgtg atcttttctt cttttgcgac
     8221 ttcttcataa atgactttgt attcttttgg cacttgccaa tcattagcga ttcctcgttc
     8281 acaacgtact ttctgattcg taaatggatc aactgcgccc attaattgtt ctttttcgta
     8341 gaaaatatgt cctgcacggt cattatccac acaaatctcg atggattgag gaaaggcttg
     8401 tttctttctc aattcagata ctgcctcacc aaaataatga ctgatcacat ggtgttttaa
     8461 cccttccatc gaaactaacc acgtatcgtt taactgatca cgatttagtg cagcatagct
     8521 aagtaaatca atactactct caaaaaattt taactgctta gggtcaccga tctttaggtt
     8581 aaagccatga ttcgctgtcg aatttttatc tatatgctta tatgtgcctc tctcaccaaa
     8641 acgttcataa tcaatgaacg ttccttgata actcgcccct actgtctcat ttgtctgtgg
     8701 atctttccat aaaaaggcga ccgcatgatc taaacgatcg tctcgaacta agaaattttg
     8761 ccgttcatgt tttggcttta gttcggcaat caacccttct ttttctgcat aatcaatcag
     8821 ccactctggt agttttcgtt cttccttcag ataattcctt cctttttctg tgagtggttg
     8881 ataatcaacg gggtgatctt tgtacttttc tgtctcaaaa tgaagatttt ccactcgttc
     8941 tgttttgtct tcattcttta gaataaaatc agtggctgct ttaaattgtt gcttagggtc
     9001 ttggatctct ttgtctatga aaaaaagttt agctaattca ataatgtcgc cactaatatt
     9061 ttgactgttc caataaaaac gttgttttct tcgatcaaaa acaaatgaat catggtcttc
     9121 taagcgatag tctcgtcctt tattgactac agccattcca ttgtttcgag caaaatccac
     9181 tatatcgatc tgtttacatc gatcaataag actcattttt ttctcggtca ttttaaaacc
     9241 tcccaaaaga aaaaagacta tccgtttttc agatagtctt ttttacttgt tttatacacg
     9301 ttctttttgt gacaattgtt gttcataata gatttcattt tttcgttttg ccacttctga
     9361 cactgtctca agtgaaaatc ggaatgaatt tcctagcgca aattcttctg aaggtaacgc
     9421 cgtttggatc tcttttccat aactttttcc ttttaaaatt acttgattat gctgtggatc
     9481 ttcaataaac gccgcatcct tcaaatgtaa cgttcgaccc gtatcttctt ccattacttt
     9541 cccgtctttc ttgtcgatcc aaagtgaacc aacaggaaca atattctttt gttctgccgt
     9601 ttctacagta tttttatgct cctgcttcac ttctgctggt tggatctctg gttctctttc
     9661 tctgtattga ttgatttcat tagaaatggt ttgaatgaat tcatttgcga cacggctgac
     9721 atcattcatg attttcccac gttccgcagc ttccttatca tccaacgtct gaccattctt
     9781 cgtccatgct gcaatataag gaatagcttt ctccgaagtg tccattccaa aatgtttgca
     9841 cacaatataa gaagtcattt ctgcctgcaa ttctttagtt gcggtatcta attctgaaaa
     9901 tttgtgcatt cttgcatggg ctaattcatg aatacttgta gctaaatgct gcgtaggagt
     9961 atttcgagta ttcatgacga tttcttcttc gccagtaaat tcgttacgaa catatgcccc
    10021 ccgtgcttgt cctaattctc tttgatagac ttcactttct gtcatatctc gaatttgtat
    10081 tccaatattg ttggctaccg cttgaatccc agcttgaagt tctgactgtc cttttttatc
    10141 caattgaaaa tcaaagacac ggttcggaaa gattttcgga tagtcttcag gttttaactg
    10201 tgtttgtgtg atatcaaaca ctttttcaat cgtgtagtaa ggttttttga tcacttcgat
    10261 ttgaccactt tcgattcgtt ttttatcttc tggtgtagct tctgtttctc gaacaaagcc
    10321 acgtcctttc tcataaaatc ctttgaccgt ttttctgaca aagatcttgt tcccttgttc
    10381 cccttttttg atatagattc cttctttttt caacgcagca cgacctaaac aagccgttgc
    10441 acctggaaat tgttcctcaa tcaataattg atttctaaag gatcgttctg gaaaacgaga
    10501 cataaaattc aaaagttctt tcacgtcttc aggagaatgg gtatgctttt tcaccgcatc
    10561 cagtgcctgt tgcgaaaggg atttgacttc ttgcttaatc tcttctgccg ttcgtttttg
    10621 atagcccttt tgttttgttt ttgtttcttt cgactgcatt tgttctttcg aaacaggttc
    10681 atttttgacc gtttgttttt gttccttttc aggtacttgt tcgcacaatt ttgctaactc
    10741 taaatagatg ggttgattac tgttgacgcc atcccctaga tccattcgga tctgatcgac
    10801 tacttctccg ttcttcactt cttcaaaata gaatttataa taacctgctt ctgcggcgta
    10861 ttggttatcc aacgttttga gttgatcgat caattctaat gttacgatct ccccaccata
    10921 acttttcaat cctttttctg gatcattttc gttgaattct actcgataga aatgatcgtt
    10981 ttctttctcc ttcaaagaat cagttggcgc agcctcttca agtaattcac gaacagctgc
    11041 tttttcctct tccgacgtgg cttggtattt ttttgattcc tcaaatcccc cgttttcatt
    11101 cagttctaaa gcaacttttc gatagtcttt caaaaaataa atggttggat tttttttcca
    11161 ttgcgtcact cgtccgctgt caataattgc ttgtacttca ggaagaagat tttcttttcc
    11221 tttttctgat ctttctttca gctgttctaa tgtttccaat ttccgttggt attttcggat
    11281 cgtagcggct gaaaaagtcg attcaccttt ttcaaagcgt tcgatttctt ctttgattct
    11341 tggaatattt tcgattgtca tatccaggcc gccgtacttg ttatagccca tttcctttag
    11401 ctcttcttgt ctttcaagtt tttctacctg tttttcttgt tgcttgatct cttctctcag
    11461 actatgcact ttgttttcta aacgttcttg ttgcctaaac caagaatgcc catttctctt
    11521 atcgttcatt ggctgaccgt tggtctttct ctggtgttca aacatcttct caatttgttc
    11581 gtttaattca ttttgcaatc gtactaattt tcttttggat tttcccaagc gactattgtc
    11641 tagtggttct ttttcttctg ccatgtc
//
LOCUS       DARBDV010000086        11602 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000086 DARBDV010000000
VERSION     DARBDV010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.088"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            6..305
                     /locus_tag="SCM56_002294"
     CDS             6..305
                     /locus_tag="SCM56_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1111040.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="SCM56_002295"
     CDS             378..1739
                     /locus_tag="SCM56_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEG1111041.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="SCM56_002296"
     CDS             1761..2021
                     /locus_tag="SCM56_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111042.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="SCM56_002297"
     CDS             2035..2787
                     /locus_tag="SCM56_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEG1111043.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="SCM56_002298"
     CDS             complement(2881..3486)
                     /locus_tag="SCM56_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG1111044.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="SCM56_002299"
     CDS             complement(3539..4513)
                     /locus_tag="SCM56_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HEG1111045.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="SCM56_002300"
     CDS             complement(4891..5610)
                     /locus_tag="SCM56_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1111046.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="SCM56_002301"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="SCM56_002301"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEG1111047.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWIVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="SCM56_002302"
     CDS             7161..8558
                     /locus_tag="SCM56_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG1111048.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="SCM56_002303"
     CDS             8639..9079
                     /locus_tag="SCM56_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEG1111049.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="SCM56_002304"
     CDS             9227..9553
                     /locus_tag="SCM56_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEG1111050.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="SCM56_002305"
     CDS             9554..10303
                     /locus_tag="SCM56_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111051.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="SCM56_002306"
     CDS             complement(10383..10838)
                     /locus_tag="SCM56_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEG1111052.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="SCM56_002307"
     CDS             complement(11265..11540)
                     /locus_tag="SCM56_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111053.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
BASE COUNT         3662 a         2013 c         2356 g         3571 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aatccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa ac
//
LOCUS       DARBDV010000087        11269 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000087 DARBDV010000000
VERSION     DARBDV010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.089"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="SCM56_002308"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="SCM56_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEG1111054.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="SCM56_002309"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="SCM56_002309"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEG1111055.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="SCM56_002310"
     CDS             complement(2169..2906)
                     /locus_tag="SCM56_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEG1111056.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="SCM56_002311"
     CDS             complement(2997..3524)
                     /locus_tag="SCM56_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111057.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="SCM56_002312"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="SCM56_002312"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEG1111058.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="SCM56_002313"
     CDS             complement(4401..4886)
                     /locus_tag="SCM56_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEG1111059.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="SCM56_002314"
     CDS             complement(5039..5236)
                     /locus_tag="SCM56_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111060.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="SCM56_002315"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="SCM56_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HEG1111061.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="SCM56_002316"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="SCM56_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HEG1111062.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="SCM56_002317"
     CDS             9211..9423
                     /locus_tag="SCM56_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEG1111063.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="SCM56_002318"
     CDS             9425..11104
                     /locus_tag="SCM56_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEG1111064.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
BASE COUNT         3271 a         2434 c         1974 g         3590 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaatacctgc cacaagaga
//
LOCUS       DARBDV010000088        11065 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000088 DARBDV010000000
VERSION     DARBDV010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.090"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(319..687)
                     /locus_tag="SCM56_002319"
     CDS             complement(319..687)
                     /locus_tag="SCM56_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEG1111065.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            837..1319
                     /locus_tag="SCM56_002320"
     CDS             837..1319
                     /locus_tag="SCM56_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HEG1111066.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1356..2366
                     /locus_tag="SCM56_002321"
     CDS             1356..2366
                     /locus_tag="SCM56_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEG1111067.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(2481..3194)
                     /locus_tag="SCM56_002322"
     CDS             complement(2481..3194)
                     /locus_tag="SCM56_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111068.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(3384..3974)
                     /locus_tag="SCM56_002323"
     CDS             complement(3384..3974)
                     /locus_tag="SCM56_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEG1111069.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            4458..4685
                     /locus_tag="SCM56_002324"
     CDS             4458..4685
                     /locus_tag="SCM56_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEG1111070.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            4701..5867
                     /locus_tag="SCM56_002325"
     CDS             4701..5867
                     /locus_tag="SCM56_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEG1111071.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            6101..7144
                     /gene="hrcA"
                     /locus_tag="SCM56_002326"
     CDS             6101..7144
                     /gene="hrcA"
                     /locus_tag="SCM56_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEG1111072.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            7238..7801
                     /gene="grpE"
                     /locus_tag="SCM56_002327"
     CDS             7238..7801
                     /gene="grpE"
                     /locus_tag="SCM56_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEG1111073.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            7852..9681
                     /gene="dnaK"
                     /locus_tag="SCM56_002328"
     CDS             7852..9681
                     /gene="dnaK"
                     /locus_tag="SCM56_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEG1111074.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            9832..10998
                     /gene="dnaJ"
                     /locus_tag="SCM56_002329"
     CDS             9832..10998
                     /gene="dnaJ"
                     /locus_tag="SCM56_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEG1111075.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT         3777 a         1863 c         2320 g         3105 t
ORIGIN      
        1 tcctaaagtg atattatatt taaatatacc tgaaaataat gggcagtatt atgaaagatc
       61 actgatattt tctcgtattt tttccttatt caaaagtttt gcaggaaaat tgctgaaaaa
      121 ataattttac tatgtagaaa attcaaagcc attcaatcag tgattttttt cagcacaaga
      181 aagactcctt ataaaaatag cagatacttt gtcaattaag tgtttaacca gggatgataa
      241 aaaggatagg attgaacaaa acctgtataa ggtgatgaat agtttcctcg ccagtaaaag
      301 gtttattcgt ataaagcttt atcgccaaaa tttatcatga ccttttttat cttcaatatt
      361 aaatgaaatc aattgttcaa tcaattccca gttgattaca cttttccaag gaactttgat
      421 gacgttatcc gtatatacgt agcctgcttc ttggatattt ttttcaaggg cacgaatcgc
      481 tgctttttcc ggtgcgatag aaaaatgatt tttagcaaaa ctaaatgcaa taataaaggt
      541 accatggtca gtaaacatag gttggttcca ttttacagtt gtgtctagct ggggatacgt
      601 ttctgttatc ttagcaaaaa ggtctttgat cttttgttga tgttcttttt ctttgatttg
      661 attgatgatc ggctgaaatt tttccattct aaaacgcctc cattaagtgg tttgatttat
      721 tttaccagaa aaacaatagt tatgtataaa aaacgaacaa aacacaagta atgttgtaac
      781 cgctatattt atatgataag atgtctcaaa taataaaata gaaaagagac ataaaaatga
      841 ttgacttggc aaagaaattc ttacacgaaa atatatattt tgagttgtat catcatcgag
      901 ccatttatac aaatgaagat gctttggtaa taaagaaaga acaaggattt acaggtacag
      961 agaccaaaag cttattctta aaagataaaa aagataataa ctacgtatat ctgactttta
     1021 ctacgaaaca aacggatttc aaagcattag ggaagatcgt cggtaaaagg ctttctattg
     1081 tttcatcaga acgcatggaa gaacaaacgg gacaaaagca gggcgctgtt tctccgtttg
     1141 gttatgaaaa acagattccg attattattg atgaagagct cttatcaaat gaaaaacttg
     1201 tttttgcacc aggtagacca gatcaaacga tggtcatcca aacggttgac ttaaacaaaa
     1261 tcatccagct acttgagatg ccgatatttt tgatgccggc aaacgaaaaa aacaattaac
     1321 caagtaaaat agtgcatact tggaggtgaa gcaacatgat aagttatcca ttattagaaa
     1381 gaaatgcaac gattggggtt actgcaccat cctcaggcgt atcagaggaa caccattcgt
     1441 tactcgaatc agctttaaag agaatggaag aaaaatcttt ccaagtagtt actggaaaga
     1501 cgctttggac gcaagataag gaaaaatcag gtccagcaag aaaacgagca gatgaattca
     1561 atcaaatgat gcaagatgaa aatatccaaa tgatctttcc gacttgggga ggagaattat
     1621 tgattgagat tctcgatttt gtcgattttg atgctctaca accaaaatgg atagtaggtt
     1681 attcagatat tagtacgttg ctacttgctg tcacattaaa aacagggata gccactgcac
     1741 atggaactaa ccttattgat ttaagaggag agtacttgga tgaaacgaca gcaatgtggg
     1801 aaagtgtact gaaaacaaga gaaggagaat cgattctaca gcaatcttct gaaaagtatc
     1861 aaaaagtgtg gccccatgag cattcatcat cttctgtttt ccatctcact gaaccaacaa
     1921 attggcaatc agtcagcgga gatgcagtaa ggatgaaggg acgattatta ggtggttgta
     1981 ttgatacgat caggcattta atcggtactc cttatggaga tgtccaatct tttaaaagaa
     2041 agtataatga tggaaagccg atcttgtggt ttctagaaaa ctgtgagcta tcgtcagttg
     2101 atttaagaag atcacttgtt cagatgaaac tggcaggctg gtttgatggc agctcgggga
     2161 tcatgttcgg tagaagtgcg gcagaggatt tgttagatta taaaatgaag gatgtatacg
     2221 aagatctgtc aaaggaatta aaactaccga tcttgtatga tattgattgt ggtcatgtac
     2281 cgccacaact gacattgatt aatggtgcct atgcagaagt agcggtagaa gatagaaaag
     2341 cgactgtttt gcaaacgttt atttaaaaaa ttttttcctg ccttggagtc tatcaaagac
     2401 caataaatct aaatagaaaa gaaagtgaaa gaagctgaca aaatgctgtc agcttctttt
     2461 tgtataacat attagaaagt ctaattatct ataactgtca catcgattaa ctgtttcacg
     2521 tcattgggat ttcctgtagg aatagctact acagatgcat tatcaatgat ttcttgagtt
     2581 gtttctctaa tgaacgctgg ataagatgtt tgatctgcag gcatttgcca atgtgaagga
     2641 actggataac tagtgattgt cccgtcatgg ttacttgaat acactaaaac tccctggtaa
     2701 ctgtatttcc cagtgagcat aaccacatct ttcggataga ctgcactccc tgcatcataa
     2761 ggggcgatag gtgttcctgc aggataatga ataacatgta attcgaaatc acccgatgta
     2821 tctctaaaag tattacctct aatagctagc caaacacgtg cgtactcaat ttgtagatca
     2881 gaatatcctg ctaacggatc tattgactga gaagactgtg aggaggttgt gtctattgtt
     2941 gaagaagaaa tatttgtctc aactgtacta ctagatgtag aagaagtatt gggctctgag
     3001 actttactgc ttgttgaaga tgtattcgtt tcagtagaag ctgatttctg agtacttgat
     3061 ttttcagctg aactggtttg tctgattgaa cttttgtctg tggtcgcaga tggatcacta
     3121 ttttctttac taggagacgt acatcctaca aggaataaag agaccaacaa taaaataaaa
     3181 cctaactttt tcatataaaa tacctcatta tttttttaat accagtatag cataatcctg
     3241 aatttttgaa tccgtaaaac cttgtctatg aatataaata gagtgtatct tctttgataa
     3301 atgtcagaaa aagtgtgaca aataatcatc agtaaaatta cttgtcacac tattgataag
     3361 gagtatttaa ttgaagagaa tgattaggag aaaggatctc cgtacttttt atagtaatca
     3421 tagaatccaa gtttgtatag acgaatcgtt ttttctctta tttcttctgt ttcaaaatgg
     3481 actgcttgaa gaatcaagtc tgtgaattct ataggagctg ttccattcgc agtaatcaaa
     3541 ttacgatctt tcacagcttg ttgttctata aaatcttctt tattctgata attttccgat
     3601 tctaaccaag gaaattgcgc atttccggtg tgtttgtaac cttgcaataa tccgctctga
     3661 gctaaataat caactgcccc acaaatagca gctactggta cgtctgtttt taacaattta
     3721 tcgattattt gatataaatg atgatttttt atgtcccatg aattgccacc gatcataata
     3781 aaaaggtcaa ctttttcagg tatttgttcg attaagtaat cgatttttgt ttttaatcct
     3841 ccaatggaag tgacctctga ttggatagaa gcagttttgt tttgccattt ctcattttgg
     3901 tttagtgtgc tagtaatata agcaccttcc caatcggcat attcctctaa tagaaacgta
     3961 atcgcagttt tcattttcaa gctctccttt actatctata acaaggacaa cagttttaac
     4021 ctacagacat tcattaaaat tgggtagcga cagcgaaaac aacaattctt aagttttaga
     4081 cagagtatac agatagtcaa cttaattaaa tacgaactaa ataaaaatga caggtaatca
     4141 tttttgttta ccagcattat aacattttgc atacatggat gacaaaagaa ccaattccca
     4201 cttgcttgag tacaatatta aaagaaactg gaaggcaaaa agtaattata gaaacgaaaa
     4261 ataaatcata tcgggcactc agacatgaaa acaaccctag agatatataa ctaggtaacc
     4321 agtttaatga aagaaaaaat catccaagaa gtcgattctt ggatttttta gtggatttgt
     4381 tcgccaaata cgtttagtat gcgtatatga gaggttgtaa gcaaagcgtt gtggtctgag
     4441 cacttaagga ggaatctgtg aaaatagctt ttcatatttt tgcagatttt gacttaatgc
     4501 cgaaggtcag ctttgcgatt accgtttatc gagaggctgt aaaagcaccg tgtagctccg
     4561 atgacaataa gcaagcattt ttagagctag gtttccttga ttctaaatat gcggcttatt
     4621 tcaatgagtt ggtgcttgaa cgcgatttta agaaggtcga aaacaggcac gacttttcga
     4681 cgtaaggaga aagaatttca atgatagaaa caataaatcc cgcagaaaac aaggtttctg
     4741 cgtacattca tatcccattt tgcgagcata tctgctatta ctgtgatttt aataaagttt
     4801 ttttagaagg gcagccagta gatgagtatg tggaaatgct gctgaatgaa atgaaaataa
     4861 tgctagaaag tcatccgaca gcgaatttag aaacacttta cgtagggggc gggacaccaa
     4921 cttctttatc tgctaaacaa cttgatcgac tgttatcagg tgctagagag atcctgcctt
     4981 ttcaaaatgg taatgagttt actgtagaag cgaatccagg tgatttgacc cgtgaaaaac
     5041 ttcaagtaat gaaaaactat ggcgtcaatc gtttatccat gggagtgcag acatttgata
     5101 atcgcttatt gaaaaaaatt ggacgtaaac acaccgcaga agatgtgtat caaactatgc
     5161 aatttttaga agcagaaaat tttaccaatg taagcatcga tttgatttat gcattaccgg
     5221 gacaaacact agaaggatat ctggatactt tggaaaaagc tttggctttg gatctgcctc
     5281 actattcttt gtactcattg atcctcgaaa acaaaacaat gttcatgaac tgggtacgac
     5341 aaggaagact cgaactgcct gatcaggaaa cggaaaccag aatgtttgaa gagacgattg
     5401 aagcaatgga aaagcatgga cgtcaccaat atgaaataag taactttggg ctctccggac
     5461 atgaatctca acataacctc atgtactgga acaatgacca ctattttggg tttggagctg
     5521 gtgctagcgg ttatcttgga gatattcgtt accgcaacaa aggaccaatc caacactatt
     5581 tacgcccatt acgagcaaat gaacgcccgg tattggaaga agaaaaattg acacgaaaaa
     5641 atcaaataga agaagaaatg ttcttaggac taagaaaaaa aacagggatt actaaaaaac
     5701 atttctatga tcgctatcaa aaaacaattg aatcggtgta cggtgatgtg ctaagggaat
     5761 tagagcaaga agggttactt gttaatgaag aagaccgtgt ttatttgaca ccaagaggga
     5821 cctttttggg gaacaatgtg tttgagcgct ttttgtttga aaaatagttt aaagaatatt
     5881 tgttttttat ctgaatttaa ataaaaaaag ttcaaaaata caataaagga ctattgaaac
     5941 gttgatataa ctggatttac atcaattatt tagaattagc actcttgtgt ttagagtgct
     6001 aaaaaatgga gtctttctct tgacaggaga agggcttctt gctatattta taagtggatt
     6061 agcacttaga atatatgagt gctaatgaga ggtgaggaag atgttaacaa agcgacaaaa
     6121 cgacatttta cgtctgatca tccaacacta tacgagtagt ggcgtgcccg ttggttccaa
     6181 aacattgatg tctgagggag tcaaggccag ctcagcaacg attcgcaatg atatgaaagc
     6241 attagaagat gaaggattgc tgttgaagac tcactcttct tctgggcgga ttccatctgc
     6301 tttaggctat cgttattacg tggatcattt gttaaaacct gctcgcgtag cagaagatga
     6361 tttgctgctg attcgtcagt ctctaggcag agaatttcat gaaatcaatg agattattaa
     6421 gcaatctgct gagatattat ctgagctgac aagctatacg actttttcat tgggacctga
     6481 aataaaagac cgccgactga ctggctttcg tatcgtaccc ttgaataatc gacagattat
     6541 tgctatcgtc gtaacagata tggggaatgt agaaagccaa gtgttcactc ttcccgaata
     6601 tatgggaagt caggaactag aaaaaatggt acagattgta aacgaccgtc tagtcggaga
     6661 tcctctgatg acggtgtacc ataaacttcg tacggagatt ccgatgattc ttcataaata
     6721 tttccaaacg acagaaggga tgtctcagtt atttgacacc gttcttggtc atgtttttga
     6781 agataaggtt tatgtcagtg gacaaatgaa tctcttggat tatgagcccc gtcaagatct
     6841 tgatcagttc aaatcgatgt tttcttttat gaaagactca gacggactta cacaaatgat
     6901 cactccgatg gatagcaata ttcatatcaa gattggtgct gaattaggca atgatttatt
     6961 gcagaatatg agtatgattc aagcaagtta cgagatagat ggtcatggaa gaggtacaat
     7021 agccttgtta gggcctacta gtatgcctta ttctcggatg ttcggtctag tcgatgttta
     7081 tcgaagagaa ctagcagcta aacttgctga ttactaccgt tcgcttgact tgtcaaactc
     7141 gtaatgtgaa taacgaagct atttgcagct gatcgttctc tctttcttag taagagcaat
     7201 caaacagctg actgaataat gaaaggaaga ctgtatcgtg aaagaaaatc aagaagaact
     7261 agataaagaa atgacagatg ctcagccaga accggaaatc gatgtcgaag ctgcagaaga
     7321 ttcgggtatt tcagaagctg aagcggaaga attcgaaaca gcgaaattaa aagctgagtt
     7381 agaagaaatg gaagataaat acttgcgggc tcgtgctgaa atcgcaaata tggcaaatcg
     7441 aggaaaaaat gagcgtgagc aattacaaaa ataccgttca caagatttag ccaaaaaatt
     7501 acttccatca attgacaacc tggaacgtgc attagccaca gaagtgtcag atgatcaagg
     7561 ggcaggtttg aaaaaaggcg tggaaatggt cttggaaagt ttgcgtaacg cactggaaga
     7621 agaaggaatt gaaaaaatcc ctgctaaagg agaagccttt gatcctaatc ttcatcaagc
     7681 agtacaaact gttccagcga ctgaagatac accagccgat acgattgtag aagtactaca
     7741 agaaggctac aaattacatg atcgcgtgtt aagaccaacc atggtcattg ttgcacaata
     7801 attgtgcgga tataaaactt acactttcaa ttaaagaagg agacgttaat tatgagtaaa
     7861 attatcggta ttgacttagg tacgactaac tctgcagtag cagtattgga aggaaacgaa
     7921 gcaaaaatca tcgctaaccc agaaggaaac cgtacaacac cttctgttgt atcattcaaa
     7981 aatggagaaa tccaagtagg ggaagttgca aaacgtcaag ctgtgacaaa cccaaatacg
     8041 atttcatcaa tcaaacgcca tatcggtgaa gcaggttaca aagtagatgt agaaggtaaa
     8101 tcatatacac cacaagaaat ctcagcaatg atccttcaat atatcaaagg attcgcagaa
     8161 gactatttag gtgaaaaagt cgaaaaagca gttatcactg tgccagctta ctttaatgat
     8221 gcacaacgtc aagcaacaaa agatgctggt aagatcgctg gtctagaagt agaacgtatc
     8281 gtcaatgaac caactgcagc tgctcttgcc tatggtttag ataaaacaga tcgcgacgaa
     8341 aaaatcttag tattcgacct tggtggcggt acttttgacg tatctatcct tgaactagga
     8401 gacggtgtct ttgatgtatt gtcaacagct ggcgacaacc atctaggtgg ggacgacttt
     8461 gataacaaaa tcatcgacta catggtagca gaattcaaaa aagaaaacag cattgacttg
     8521 tctcaagaca aaatggcttt acaacgcttg aaagacgctg ctgaaaaagc gaaaaaagat
     8581 ttatctggcg tatcaagcac acaaatcagc ttgccattta tcacagcagg agaatctgga
     8641 ccattgcatt tggaaatgac attgacacgt gcaaaatttg atgaattaac agctgactta
     8701 gtagaacgta caaaagtacc agtacgtcaa gcgctaaaag atgcaggttt atcacaatca
     8761 gaaatcgacg aagttatttt agtcggtgga tctactcgta tccctgcagt tgtagaagca
     8821 gtacgtaaag aaacaggaaa agaaccaaac aaatcagtta accctgatga agtagttgct
     8881 atgggtgctg ctatccaagg tggcgtgatc actggtgatg tgaaagatgt tgttttactt
     8941 gacgttacac cattgtcatt aggtatcgaa acaatgggtg gagtgttcac taaattgatt
     9001 gaccgcaata caacgattcc aacaagtaaa tcacaagtat tctcaactgc ggcagataat
     9061 caaccagcag tagacatcca tgtattacaa ggtgaacgcc caatggctgc tgacaacaaa
     9121 actctaggaa gattccaatt gacagatatt ccagcagctc ctcgtggtat tccacaaatc
     9181 gaagtaacat tcgatattga taaaaacggg attgtaaatg tatctgcgaa agatctaggt
     9241 acacaaaaag aacaaaaaat cacgatcaaa tcttcttcag gtctaacaga cgaagaaatc
     9301 gaacgtatgg taaaagatgc agaagcaaat gcagaagcag ataaagctcg taaagaggaa
     9361 gttgacctac gtaatgatgt agatgcattg ctattctctg tggacaaaac attgaaagaa
     9421 ttagaaggca aagtggacga agaagaagtg aaaaaagcag aaacagctcg cgatgaattg
     9481 aaagccgctg ttgaagcaaa caacatcgaa gaaatgaaaa ctaaacgcga tgcattgaac
     9541 gaaatcgttc aaaacttgac tgttaaacta tacgaacaag cggcacaaca acaagctcaa
     9601 gaaaacccag aagcagctca aggcggagca gatgatgtcg ttgatgctga ttttgaagaa
     9661 gtcgatggcg acgataaata atcagttcac tgaatttcga gagaaggcca aagttgctac
     9721 tttggctttt tcttttgaaa aatagtttga agtatcggtt tttgtttcaa acaaaatatg
     9781 atatgataac aagcgacgtg aaaatgatga gaaccaatag gaggaaagcc tatggcaaca
     9841 aaacgtgatt attatgaagt cttagggctc tcaaaaggag catcagaaga tgaaataaag
     9901 aaagcttatc gtaagctttc gaaaaaatac catcctgaca tcaataaaga acctgatgcg
     9961 gaagaaaaat tcaaagaagt atcagaagca tatgagattt taagtgaccc gcaaaaacgc
    10021 gctgcatatg accaatacgg gcatgcaggc acagatccaa attacggtgc tggtggagct
    10081 ggtggcggat ttggtggatt cggcggcttc tctggtggcg gcttcggtgg ttttgaagat
    10141 atcttcgact ctttctttgg aggaggcggt cggtcagtcg atccaaatgc gcctcgtcaa
    10201 ggtgctgatt tgcaatatac ggtcgacttg tcttttgaag aagcgatttt tggtgtagaa
    10261 aaagaaatca agtataatcg ggaagaaatc tgtcatactt gtggtgggaa cggtgcaaaa
    10321 ccaggcacac aaccaactac ttgtcataaa tgtcatggtg caggtacgat taatgtagaa
    10381 cgccaaacac cacttggacg agtgatgagc cgtcaaactt gtgatgtttg tcatgggaca
    10441 gggaaagaaa tcaaagaacc ttgtccaacc tgtcatggct caggacatga gaagaaagca
    10501 cattcagtta aagtgaatgt accagctggt gtagaagaag gacaacaaat gcgtttagct
    10561 ggtcaaggag aagctggaga aaacggcgga ccatttggtg atttgtatgt ggtcttccgt
    10621 gtagaagaaa gtgatatctt tgatcgtgaa ggttctgaaa tttactatga attgccttta
    10681 agttttgtac aagcagcttt gggggatgaa gtccaagtac caactgtcca tggagatgtg
    10741 aaattgaaga tccctgctgg aacacagaca ggtacgaact tccgtttacg tggaaaaggc
    10801 gctccgaagt tacgtggtgg tgcaacaggc gaccaacatg tcaaagtaaa actgatcaca
    10861 ccgaaaaatc taaatgaaca acaaagagaa gcattacgtg cattcgctga agctggcggc
    10921 atcaaagttg aagaacagca agaagacggt ttcttcgata agtttaaaga tgcttttggc
    10981 gggaaaaagc ggaagtagtt tttctagtaa aaacgctaag tcattaataa ttaaaaacca
    11041 gacgaacaat catatgttcg cctgg
//
LOCUS       DARBDV010000089        10923 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000089 DARBDV010000000
VERSION     DARBDV010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.091"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..281)
                     /locus_tag="SCM56_002330"
     CDS             complement(<1..281)
                     /locus_tag="SCM56_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1111076.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEA"
     gene            423..1541
                     /locus_tag="SCM56_002331"
     CDS             423..1541
                     /locus_tag="SCM56_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEG1111077.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1669..3045
                     /locus_tag="SCM56_002332"
     CDS             1669..3045
                     /locus_tag="SCM56_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEG1111078.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3446..5026
                     /locus_tag="SCM56_002333"
     CDS             3446..5026
                     /locus_tag="SCM56_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEG1111079.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            5224..5447
                     /locus_tag="SCM56_002334"
                     /pseudo
     CDS             5224..5447
                     /locus_tag="SCM56_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(5652..5972)
                     /locus_tag="SCM56_002335"
     CDS             complement(5652..5972)
                     /locus_tag="SCM56_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEG1111080.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(6120..8354)
                     /locus_tag="SCM56_002336"
     CDS             complement(6120..8354)
                     /locus_tag="SCM56_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1111081.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8833..9099
                     /locus_tag="SCM56_002337"
     CDS             8833..9099
                     /locus_tag="SCM56_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEG1111082.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            9099..10826
                     /gene="ptsP"
                     /locus_tag="SCM56_002338"
     CDS             9099..10826
                     /gene="ptsP"
                     /locus_tag="SCM56_002338"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEG1111083.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         3480 a         1911 c         2215 g         3317 t
ORIGIN      
        1 gcttcttcaa tcaatatttt tcctaaccct tggtgtctgt attcagaaag aatcgccatc
       61 cgctgtaatt tcatttttcc gttttctaaa ggcaaaagac gtacagttcc ttgcggttct
      121 tttttatctg agtacaggac gaagtggata caatgggctt catctttgtc tatttctcga
      181 gacaaaggca caccctgttc gactacaaaa acttgatgcc tgatccgtac agcatctaag
      241 taaatattgc tcattgtatc ttttgtttgt acaactttca tggtctagcc accttccttt
      301 cgtatagtat actggaaaat aaaacaaata aaaagtatgg caagttttca aaaataaaaa
      361 aagaataaaa gaatttttaa cgaatataat atatactagt aagaaagaaa ggaggaaatc
      421 tattgtttga aaaacttaga cagtctaaac taatgttttg gtcgcttgaa ttattgatca
      481 tagctgcatt gatcatgatt tcatcagaaa taaactttat tttcgaaccg atcgggacat
      541 ttttttccac gttatttgca cctgtactga ttgcaggctt tctttactat ttattgaatc
      601 ctattgtcaa gctgttgatg aaagcaaaaa tcaaaagaat atgggctgtc gcaattgtgc
      661 tgctcctttt agttgcagca attatttgga ttttacttag tgtcatccca agtctagtac
      721 agcaaatttc ctcattggca tctaatatgc ctgatttcat caaacaggta gaaagctggt
      781 taaaagaggt agccgaattg cctttgttca aggaagtaga tatcaataaa tattttgagc
      841 aattagacat ttcttacggg acaatcattc agcaattcct tagtggagta tccaatagtt
      901 taggttcgat cgttggaacg atcgcttcgg caaccgtagt aatcattaca gcaccattca
      961 tcttgttcta tatgttgaaa gacggagaaa aactagtgcc aaacatccaa cagtttttcc
     1021 cagaaaaaag aagaaaccaa attgtggagt tattggggca actgaataaa acattggcta
     1081 attacattag cggacaagca atcgaatgtt tattcgttgg taccttcaca tttttaggat
     1141 atatggtaat tggtgtggat tatgctttct tatttggggt cattgcggga ttgacaaacc
     1201 taattccata tttaggacct tatctaggac tagcacctgc atttctagta acagtcttca
     1261 atgatccggt aaaagcatta ctttgttgtg tggttgtgct aatcgtccaa cagttggatg
     1321 ggaatatcat ttatcctaat gtaattggga aatcactgaa aatccatcca ttaacgatca
     1381 ttatcgttct tctagtagct ggaaatattg ccggattgct tggtatcttc ttaggtgttc
     1441 ctttttacgc aatctgcaag acgatcattt cctatataat caaaatcgtc aaagaagaca
     1501 agcaaaatga aaacaagaag aaaattgcta cgacctcgta ggtagtttta gtacacttgt
     1561 ttttattgca aagaaaattg ctttgtgata ccattttaac gtggctatag tgccacgttt
     1621 ttttctatcg taaactaaaa attaatttta taaggagaga atgcgattat gaatgctgac
     1681 cctgagagtc agtcgctgtt agcgcagatt ttattattga tcgttttgac attgatcaat
     1741 gcttttttag cagcttccga aattgctgtt gtttcgatca ataagaatcg aatcgagcaa
     1801 aaagctgatg aaggagatgt gaaatctcaa aagcttttaa aaatattgca gaatccaaat
     1861 aatttcctat cgacaatcca agtaggaatc actttggtca acattttgtc tggtgcttca
     1921 ctagctaata ctttatcgga acggttagct ccggtattag gtggcggcac agcggcaaaa
     1981 aatattgcaa atatcattgt gctggctatt ttgacctatg tatcgattgt ttttggagaa
     2041 ttgtacccta aacgtattgc tttaaataaa tcagaagaag tcgcaaagtt cacttcaggg
     2101 ctgatacgct tgattggtgt agtagctaag cccttcgtct ggctgctttc tgcttccaca
     2161 agtttattgg cgcgtatcac gccgatgact tttgatgatg aagattcaaa aatgaccaga
     2221 gatgaaatgc gttatatgct tgaaaacgaa ggcgtattga ataatgaaga attagaaatg
     2281 ctccaagggg ttttttctct tgatacgaaa gttgcccgag aagtcatggt cccacgaacg
     2341 gatgcattta tgattgatat caacgacact gtggaagaaa atgtgaatga agttttatcc
     2401 gaaaactatt caagaatccc tgtttataac gaagacaaag ataaagtagt cggaatcctt
     2461 catacaaaga atttactaaa agctgcccat aaattcggct ttgataattt agatatcaag
     2521 aaaatcatgc aggagccttt attcgttcct gaaacggtct ttattgatga tctgttatat
     2581 gaaatgaaaa agacgcagaa tcagatggct attttattag atgaatatgg cggcgtcgtt
     2641 gggttagcaa cactagaaga ccttttagaa gaaatcgtcg gagaaatcga tgatgaatca
     2701 gatgaagttg agaatcttta tgaaaaaatc gatgaacacg aatatatcat ccaaggacgt
     2761 atgctgattg atgagttcaa tgaagctttt gacagtgatc tgcatatgag tgatgtagat
     2821 acgatggctg gttatttgat tacagcatta ggcatgattc cggatgaagg cgaaaagtta
     2881 tcgtttgatg tcgataatat tacgctggtt tctgaagaaa tggaaggttc tcgaatcctt
     2941 aaaatccgtg tcatttttca tgatccggaa gaaacagaag cggaacctga agaagaacga
     3001 agatacttca gaaaagaatt cgaagatgac gagccaagaa gataattgga aacttggtta
     3061 tgaaagacgt gttgcttgaa taaacaagca acacgtcttt tcttttagtc agctgacaaa
     3121 aaaatatttg actcttatgg tttggtggct ttttcattcg ctgaatccat gctatactga
     3181 ttcagttgta gtagttgaca aaggttgtga aaaagctatc ctgcattaag taataagagc
     3241 agccaagcaa aaacagcttt tcgtattttt ggcttgggtg ggcttattgc tgataggctg
     3301 tctctagaac gccgtttact cggaaaaaga gactgctaca cggcttttgt cacagcccta
     3361 tatgaaattg tctttcttta agagatcgga caatctgtgc ctagagaaaa tataaataat
     3421 tgaatggaag aaatggagtt aggttatgaa taatccaaaa ttaaagcaac aagtggacaa
     3481 tcgcagaact tttgcgatca tttcccatcc ggatgctggg aaaacaacga ttacagaaca
     3541 attactattg tttggtgggg ctatccgtca agccgggacc gtaaaaggca aaaaaacagg
     3601 taattttgcc aaatcagact ggatggaaat cgagaaacaa cgtgggattt ctgttactag
     3661 ttctgttatg cagtttgatt atcaagggaa acgagtaaat atcttggata caccaggaca
     3721 tgaagatttt tctgaagata cttatcgtac tttgatggcc gtggatagtg cggtcatggt
     3781 aatcgatagt gcaaaaggga tcgaagcaca gacaaagaaa ttattccaag ttgtaaaaaa
     3841 acgtggcatc ccgatattta cttttatcaa caaactagat cgtgatggtc gtgaaccatt
     3901 ggaattgtta gaagaattag aagagctatt agatatcgaa tcttatccga tgaattggcc
     3961 aatcggtatg ggtaaagggc tagaaggtct ttatgatatc taccataatc gtgtagagtt
     4021 ttatcgtccg gaaaattatc aggatgaacg attagtagaa ttggatgaag atggactgct
     4081 tcccgaaaat catcctctaa ctgaaaattc cttatatgaa caagtcttag aagaagtaga
     4141 actaatcaaa gaggctggag atgcatttaa tcaagaaaaa attgctcgtg gcgatcagac
     4201 accagtattt tttggttctg cattgactaa cttcggtgta cagacttttt tagagacttt
     4261 cgttgaattg gcaccagctc cttatggaca caaaacacaa gatgatcaga tggtaagtcc
     4321 ttatgaggaa gaattttcag gtttcgtatt caagatccaa gcaaatatga atccagccca
     4381 tcgtgaccga atcgcttttg tccgaatctg ttctggaaca ttcgaacgag gaatggatgt
     4441 ctggttggaa cgtacaaata aaaaactgaa attaagcaat gtcacacaat ttatggcaga
     4501 ttcgagagaa aacgtcgaaa aagcagtagc aggcgatatc atcggtgtat atgatacagg
     4561 caattatcag attggggata cattgttcga aggaaaacta aaagttgctt atgaagaact
     4621 cccttcattt actccagagc tgtttatgaa agtgactgca aaaaatgtca tgaaacaaaa
     4681 atccttccac aaaggaatct atcaattagt tcaagaagga gcgattcagc tttataaaac
     4741 atatctaacg gaagaatata tcattggagc tgttggtcag ctacaattcg aagtattcca
     4801 atatcgtatg ttaaatgagt ataacgccga agtaatcatg tctccaatgg gacacaaaat
     4861 cgctcgctgg atcgatccag aagatttgga tgagaagatg agttcaagtc gcaatatctt
     4921 ggctcgtgac cgtttcgacc aaccgttgtt cttattcgag aaccaatttg cagaacgctg
     4981 gtttgcggat aaatatccca atgtaaaatt aaaaagtttg atgtaaagaa gtcttgacac
     5041 aagctgccct gcatcaagta ataagaatag ccaaacaaaa acagcctctc atattttgtt
     5101 tagagccgtg acggattttc cgtgacggct ctttttacta tattaaaatc atagaaatgt
     5161 ctaatcaaaa aaatataaaa attattttgc gtacatatga ttaaagaaag gagagtgtga
     5221 cacatggaaa cagaatggca aagacgtgaa agaaaaaaac agaaaaagaa gagatgttac
     5281 gattatgtcg attattagat gtaaaagact gggacaaaaa cgagcaaatt gcacaaaaag
     5341 tacgatcaat catccataaa gaggaaaaag ttaaaaataa agacacgcgt agaactgaaa
     5401 atgaaaagag aaaagtacaa aagaaaagag aaaaatcaaa aagctagaaa gaaaaagagg
     5461 ctgagacaga agcgtttaac tccaagaaat aagctggaat tcacaaaaat ttgaaaagca
     5521 attttcgtga atttcagctt atttccgaag gagttgcttc tgctttcgcc gtttatacgt
     5581 gtttagagcg tgagacaaaa gtgttcttta cttttgtccc acgctcttta attattatgt
     5641 tcaaaatttg tttacacggc taatgtagcg cctaattcat gttttggaat cgagatttca
     5701 acaaggcctg ccaattcgat tactttgatt tcatcctcgt ggtaaacggc gtctttgatt
     5761 ttatctaaga aataattttt agcatcttcg atttctttcg gctggatttt acgtttgtca
     5821 ttgatcagca gaatttcatt atgttcaggg gcttcagtgt agacaatagt ggtatttccg
     5881 gctgtgtaga ccttgactag atgggcatca gtgttttcaa gctggtttaa tacaagacgg
     5941 gaatggctgt ttgttacatt taccaatttc atggcgctca cctcttttat gtatttttaa
     6001 ttgcttgttt ctcttattat aatttctttt ttataaaatg aacagtattt tgacttttct
     6061 gaaaaaagaa tgagacggca tttctcaaag aaataccgtc tcacgctata aaactggatt
     6121 tattcagccg tttcttcggc tgattcttta gccaaacgtt ctggtttaga agtaacgatg
     6181 atttttccat ctttatccag ttttgctttc aactcatgga tagaagggtg atcaagataa
     6241 aattcagcga taccatcttc gatttgttct tggatcgtgc gtcgaagagg acgagcaccc
     6301 atagatggat catagcctaa gtcaactaat ttttctttga cattcgtagg aacttcgatg
     6361 tgaagttgct gtgctgccag catttggttg acatcgtcta gcattagtgt gacgattgtc
     6421 atcaagtttt cttttgataa tgcactgaat tcgatgatcc cgtcaaaacg gttcagaaat
     6481 tctggtgtaa agtagttgtt taattggttc aatacagaac gagtaacacc ttcgcgtgcc
     6541 gcaccaaaac caacgtttgc ttctacttta ccagttccgg cattacttgt cataatgatg
     6601 atcgtatctt taaagctgac cgttcttcct tgtgcatctg tcaatcgacc atcatctaag
     6661 atttgcaaga acatatgcaa aacatctgga tgagcttttt ctacttcatc taacaagaca
     6721 agactgtaag gatttctccg tactttttct gttaattgac cagcttcttc gtatccgaca
     6781 taacctggag gagaaccaat caatttagag acactatgtt tttccatata ttcagacata
     6841 tcgaaacgaa tcattgaatc ttgtgaaccg aatagttcgt aggcaagttg tttcgcaagc
     6901 tctgttttac ctacaccagt tggtccaaca aataagaagg agccgattgg acgattctgt
     6961 ttgtttaatc caacacggtt tcggcgaatc gctttcgcta ctcgatctac agcgttatct
     7021 tgtccgatga catgagcttt caagtcatct gctaagtttt tcagttgtgt ctgttctttt
     7081 tcttttaatt cacccactgg aatacctgtc cgttgttcaa cgattttttc catatctttt
     7141 tcggtaatca caggtgtttc ctcttcagtg agctgacgtt ctttcatttt ttgaagttta
     7201 ttgatctgat cgcgataata agctgctttt tcgaaatctt cttctcgaga agctaaaact
     7261 ttttgttctt ctgcttcttg cagttttttc tcgattgttt taggatcaac gatttggatc
     7321 gtcaagtttt tcttagaacc tgtttcatct agcaaatcga tggctttatc cggcaagaag
     7381 cgatcttgga cgtaacgatt ggataaagtt gcggctgctt cgatggcctc atcagtatat
     7441 tttacatgat gatagtcttc ataacgtttt tgcaaacctt tcaaaatact gatcgtttct
     7501 tcgacagttg gttcatctac ccgtacaggt tgcatacgac gttctaaagc agcgtctttt
     7561 tcgatgatgc ggtactcatt taaggtagta gcaccaacca tttgcaattc tcctcgagct
     7621 aaagctggtt ttaaaatatt accagcatcc atatttccat caccagcaga accagcaccg
     7681 acgatttcgt gaacttcatc tatgaatagg atcacatttt ctgcttgacg gatctcttca
     7741 atcaattttt gcatacgttc ttcaaattgt cctcgtatac cagtaccttg gactaaagat
     7801 acgacatcta aacgaatgac ttctttgtcc attaattttt gcggtacatc accatctacg
     7861 attttttgtg ctaaaccttc aacaactgct gttttaccaa cacctggttc tccaatcaag
     7921 acaggattgt tcttggttcg gcgatttaaa atttcaatga ctcgtttgat ttcgtcatcg
     7981 cgtccgatca caggatcgat atcaccttgt cgtgcttgtt ctgtgatatt gattccgtat
     8041 tctcccaata aaccttctgt attgccttga ggtggctgac caccattgaa gttgttgttt
     8101 ccgccaaatt gtgttggtgg tgtttgctca taatttcctt gctgttgcat ttggcgagac
     8161 atggaacgga aaagatcgtc caaactgcca aatccaaatg gatcattttg ggccatatta
     8221 gtcagacctc cattttgttg atttttgatt ttttgataac agctctgaca gtaatcgagt
     8281 tgtgtccgct gaccattgac agtggcatac aagtgaatcg ttgcctcatt cttgccgcag
     8341 ttttgacaaa gcatgaatca ttcacgtcct ttcacaaaaa tgggtacatc catattttac
     8401 atctcgtttt tcatgaggta aaagaataac ccctagtttt ttaagagatt tatgaggtag
     8461 aaaaggtgaa atctctattg accattcctg acctttgact cttattatac taaaaataaa
     8521 aagggaagca agtaattcgt ttcatatgaa aacaaagagg gtcaaaggaa agaatgataa
     8581 acgtcaggag aagagaaagc gacaagaaaa aggaaataaa cacgtttgtt ataaaatgtg
     8641 aagaaaagga tgaaaaagaa tccggaataa tttgaatact tttatctgaa attgaatgaa
     8701 aactgttgta tctaacgaaa aaaaatgtta atctttacag atgaaagggt ggcatttgtt
     8761 aggattctct gaattactaa aaaagaaaac cctcacataa aaacaacact cacttaaagg
     8821 agaccgatta atatggaaaa gaaagaattt cacgtagtag cagaaactgg gatccacgct
     8881 cgtccagcta cactattagt acaaacagca agcaaattta actctgatgt aaacttagag
     8941 tataaaggta aatcagtaaa cttgaaatca atcatgggcg ttatgtcttt aggtgtaggt
     9001 caaggttctg atgttactat tactgcagaa ggtgctgacg aagctgatgc aatggcagca
     9061 atcgttgaaa caatgaagaa agaaggctta tctgaataat ggttgaaatg ctaaaaggga
     9121 ttgccgctag tgacggtgta gccgttgcaa aagcttacct gctagttcaa ccggatttaa
     9181 cattcagtaa ggcaacagta gaagatactg cggctgaaga agctcgtttg gatgctgcat
     9241 tagctaaatc tactgaagaa ttgcagcaaa ttcgtgaaaa agcagcgcaa agcttaggtg
     9301 aagcagaagc gcaagtattt gatgcccatt tgatggttct ttcagatcca gaaatggtag
     9361 gtcaaatcaa gcaaaatatt aaagacaaca gcgtaaatgc tgaatcagcg ctaaaagaag
     9421 taacggatat gtatatcggt atgttcgaag caatggaaga taatgcttat atgcaagagc
     9481 gtgctgcaga tattcgtgac gttgctaaac gtattttagc gcacttatta ggcgttactt
     9541 taccaaatcc atcaatgatc aacgaagaag tggtcgttgt tgcacatgac ttgactccaa
     9601 gtgatacagc acaattagat cgcaattttg taaaagcgtt tgtaacagac attggtggac
     9661 gtacttcaca ttctgctatc atggcacgtt ctttagaaat tccagcaatc gttgggacaa
     9721 aagaaattac tgctaaagtc aaagaaggcg atattttggc tgtcaacggg atcgaaggcg
     9781 atgtaatcat cgatcctaca gatgaacaaa aagctgaatt tgaaaaagca ggtgcagatt
     9841 acgcagcaca aaaagcagaa tgggaaaaat taaaaaatgc agaaactgtc acagctgacg
     9901 gcaagcattt tgaattagct gcaaacatcg gaacaccaaa agatctagtt ggtgtccata
     9961 ataatggtgg agaagcagtt ggtctgtacc gtactgaatt cctgtatatg gattctccag
    10021 acttcccaac agaagatgac caatatgaag cttacaaagc tgtattagaa ggtatggaag
    10081 gaaaacctgt agttgttcgt acaatggaca tcggtggaga taaagaacta ccatatctgc
    10141 aattacctca tgaaatgaat ccattcttag gatatcgtgc attgcgtatc agtttatctg
    10201 aacaaggcga cgaaatgttc cgtacacaaa tgcgtgcatt gcttcgtgca tctgttcatg
    10261 gtaatttacg tatcatgttc cctatggtag ctacactcaa agaattccgc gcagcaaaag
    10321 cgatctttga agaagaaaaa caaaaattga taagtgaagg caaggaagta tctgatacga
    10381 tccaagtcgg catcatgatc gagattccag cagcagcagt cttagcagac aagtttgcaa
    10441 aagaagtaga tttcttctct gtaggtacga acgatttgat tcaatacaca atggcagcag
    10501 accgtatgaa cgaacgcgtt tcttacttgt accaaccata taacccatca attttacgtt
    10561 tgatcaaaaa tgtaattgat gcagcacacg ctgaaggaaa atgggcaggt atgtgtggag
    10621 aaatggctgg agatcaaaca gctgttccat tgctagtagg tatgggtcta gatgagttct
    10681 caatgagtgc gacatctatt cttaaaacgc gtagtttaat gaaacgcttg gacacagcga
    10741 aaatggctga acttgctgat cgtgcattga aagaatgcga tatgatggaa gaagtcgttg
    10801 aacttgttca tgaatatgta aaataaacaa cgtatcaaaa gctggtgtgg cttgtaaaag
    10861 agctacgcca gtttttttgt tcttttttaa gaaaacgttt ttgtcagtaa gaatagacaa
    10921 aaa
//
LOCUS       DARBDV010000090        10817 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000090 DARBDV010000000
VERSION     DARBDV010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.092"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(25..162)
                     /locus_tag="SCM56_002339"
     CDS             complement(25..162)
                     /locus_tag="SCM56_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HEG1111084.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            complement(159..1343)
                     /locus_tag="SCM56_002340"
     CDS             complement(159..1343)
                     /locus_tag="SCM56_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEG1111085.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="SCM56_002341"
     CDS             complement(1599..1853)
                     /locus_tag="SCM56_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111086.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="SCM56_002342"
     CDS             complement(1878..3020)
                     /locus_tag="SCM56_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEG1111087.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="SCM56_002343"
     CDS             complement(3080..4432)
                     /locus_tag="SCM56_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEG1111088.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="SCM56_002344"
     CDS             complement(4503..4847)
                     /locus_tag="SCM56_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEG1111089.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="SCM56_002345"
     CDS             complement(4857..5231)
                     /locus_tag="SCM56_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG1111090.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="SCM56_002346"
     CDS             complement(5244..5558)
                     /locus_tag="SCM56_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG1111091.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="SCM56_002347"
                     /pseudo
     CDS             complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="SCM56_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            complement(8976..9638)
                     /locus_tag="SCM56_002348"
     CDS             complement(8976..9638)
                     /locus_tag="SCM56_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111092.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            10255..10485
                     /locus_tag="SCM56_002349"
     CDS             10255..10485
                     /locus_tag="SCM56_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111093.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            10491..10790
                     /locus_tag="SCM56_002350"
     CDS             10491..10790
                     /locus_tag="SCM56_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111094.1"
                     /translation="MLFNETQLWFKFDPTNRFIKDFYKVWNSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT         3000 a         2387 c         1833 g         3597 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccggaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcggc atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacattagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcacctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atccaatgtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc actctctttt gaattggcat
     8221 ttggagtaat cactagttga ttgccgttca cacgataatc aatatttgcg atattctcga
     8281 caacttcatc aaactccgat aaattcaagc cgttcgtatc tgtcacagta gtcgattgac
     8341 ctaacacggt tttcactgtg gagttatgga agcttggttt cttttgataa tccgcaatgg
     8401 cttgattgat ttttgactca atggctgcga tatttactgc actaccatca gaacgtttaa
     8461 ttgagtgaag cgtagccatt aacttcttgc caaatcatct tctgggccac aatatgtgta
     8521 tccacatccg taccagcttc cttccataaa acggaaacta atttcgcttt ctccgacata
     8581 tcaggcaatg gatttttctc ataaccagga gtgaactcat tgtaaatcgg cacccctggc
     8641 tcaatacaga aaatatcttg cttcacacca ttgtaaactg catagtgcgg aacggtaact
     8701 tctgacatgg gacgaccatc gctaaatgta ccttcgacag catactggtg agccaaatcc
     8761 aattccacag ttaccgtttg tggatgagta atttcatcag caaaggcaat cgttgggcca
     8821 agcgatccac caattgtttg gcataataga ctcagtgttg cgagcaaacg ccaattctta
     8881 aatcttggct ttttcataaa ataaatctcc tttcgttttt gggtaataaa aaagacgtcc
     8941 aaattttaat tttgaacgtc tgatttagag tgatactatt tagttttctt tgtcttggat
     9001 aggttgtttc tctgcttata aattttgatt cttcgataat caaatccaat ctctgccatc
     9061 aattcctccc aatttttgtt cgttcttttc ataattgtat caactgatgg tagttttggg
     9121 gattgaacta gttttcgaaa ctttgtcatt gataaatcat cctgtagttt taatcggatt
     9181 atttcatttt ttagttctaa cagcagttcg aaatcagtaa atcgagatcc atcatttttt
     9241 tctttaaaaa gtggacgtat atctcctaac atctttttaa tggtactaag tgaaggaata
     9301 ttcttcccta ctgatttttg ttcgtacatt cgttgagagg ttatcttctc gcacttaata
     9361 aaggcttcta ctatttccaa taattcttgt tctgacattc tagcccattt tccataccca
     9421 gtattttctc gtcctatact aatcaataaa ttctcccatg atccatattt tttgttaatg
     9481 aaccacatac ttggtgcaga atttggattt tctgatagct ttttctggta ttgacggcta
     9541 gatgtaattc cgtgtttttc gatgaattca ttaattgtta aaatcagttt taactcactt
     9601 tgatttttcc aaatgatacg tccatcattt tgcttcacga taaaaactcc taatagttat
     9661 ttagatagaa gcctatcaca tcaacttttt agtagaaaag tacatgtttt gtcttttttg
     9721 ctaatagctg accctttgag aaacttgggt tcaaaagttg ctaaattcgg taaatgtttt
     9781 ggtatttttc gtattatatt tgtcgtcaag ctgactacta aattttgccc tattttgacg
     9841 acactgatag tcaatgtcgg acaatctagg acaattttta agggaatatg attgaaaaat
     9901 ggtccaaaac agccaaaaat ggcttaaaac tgtgatagag aaaaaagaaa aagcccttga
     9961 tatacaagga cttttcacaa atttcagatt tccaaggaag ttttccgtgt ttatcttctg
    10021 atttctttga tacgagcagc ttttccgtgt aatgcacgta aacaaaacca ctatattcta
    10081 ttatgtatct aataccctca aaacagctat aaatcaacaa taccgacata taataaacac
    10141 tatgttcaat ctgagatata tcaaagataa acgtattttt gccccctttt tgcccctcaa
    10201 atatcgcttt acaacgagaa cgtaagttcg tataatcgtt ttgaggtgat ttttatgatg
    10261 gaagacttta ttagaaaaaa tatcagtgat gaatatgcag acttttatga gcaaagcagc
    10321 aaaaaagata aattccagat ggatgtttca attttagcta tattagcttt ttccgaaaat
    10381 aaacaacctg taactgcaaa aaaagaaaca gtattctctg aaggcaaaat aaaaactcga
    10441 tatatattag aggtagaaac taattttaaa aacagatcgg agtaatggtt atgcttttta
    10501 atgagacaca attatggttt aaattcgatc ctacgaatag atttatcaaa gatttttata
    10561 aagtatggaa ttcagaagtt ttctttttag caatcgaaga tagcttatta atcaatctct
    10621 actattctaa taagaactat ttcaaaattc ctgctgcgaa aactagaatg aagaagggtg
    10681 tatacttttt gtttgatatc gtgactgacg tgccggacgc acgtagcgat catcggcgtt
    10741 atgactatat aaagtatact ttcgttgatc cagaaagata caaagattaa ataaaaaaat
    10801 aacaaacaaa ctcgctt
//
LOCUS       DARBDV010000091        10725 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000091 DARBDV010000000
VERSION     DARBDV010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.093"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(243..1091)
                     /locus_tag="SCM56_002351"
     CDS             complement(243..1091)
                     /locus_tag="SCM56_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG1111095.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1090..2157
                     /gene="gnd"
                     /locus_tag="SCM56_002352"
     CDS             1090..2157
                     /gene="gnd"
                     /locus_tag="SCM56_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEG1111096.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            2159..3160
                     /locus_tag="SCM56_002353"
     CDS             2159..3160
                     /locus_tag="SCM56_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEG1111097.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3157..3441
                     /locus_tag="SCM56_002354"
     CDS             3157..3441
                     /locus_tag="SCM56_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HEG1111098.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3474..4271
                     /locus_tag="SCM56_002355"
     CDS             3474..4271
                     /locus_tag="SCM56_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEG1111099.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4285..5118
                     /locus_tag="SCM56_002356"
     CDS             4285..5118
                     /locus_tag="SCM56_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1111100.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGGFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5136..5993
                     /locus_tag="SCM56_002357"
     CDS             5136..5993
                     /locus_tag="SCM56_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG1111101.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6015..6524
                     /locus_tag="SCM56_002358"
     CDS             6015..6524
                     /locus_tag="SCM56_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1111102.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6561..6962
                     /locus_tag="SCM56_002359"
     CDS             6561..6962
                     /locus_tag="SCM56_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111103.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7074..7427
                     /locus_tag="SCM56_002360"
     CDS             7074..7427
                     /locus_tag="SCM56_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111104.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7461..7904
                     /locus_tag="SCM56_002361"
     CDS             7461..7904
                     /locus_tag="SCM56_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEG1111105.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7921..9006
                     /gene="uxuA"
                     /locus_tag="SCM56_002362"
     CDS             7921..9006
                     /gene="uxuA"
                     /locus_tag="SCM56_002362"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEG1111106.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8999..9712
                     /locus_tag="SCM56_002363"
     CDS             8999..9712
                     /locus_tag="SCM56_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEG1111107.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(9919..10068)
                     /locus_tag="SCM56_002364"
     CDS             complement(9919..10068)
                     /locus_tag="SCM56_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111108.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            10464..10664
                     /locus_tag="SCM56_002365"
     CDS             10464..10664
                     /locus_tag="SCM56_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG1111109.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3589 a         1695 c         2320 g         3121 t
ORIGIN      
        1 acaaaaataa taaaaaaaag aaaagataaa ttttttacta ctgtatacta actttgttat
       61 cgcatctaac aagtaactcg aataatacca atacaacctt ctccaaagat gcgatgacat
      121 acgttctaac ccaccgacta ttgatgttct gtaccaccct tgcagaacca aaaaagccac
      181 taccaaatat ttggtagtgg ctttttttgt tttatggatt tttcgctttt tctgttatgg
      241 tgctattctt tgatttcaga aatagtatgt cgaactttag cgatagaacg acgtgattct
      301 ttttcgttgt aaaacatcaa aatcacatat agagagtcca aaatgcttag ttgagaaata
      361 cgggaagcta aagcttcaga tcggaattct gtttcttcag aaatagaaat aaaaacgaca
      421 tcgccaagtt tagcaaggga agaattagct tggctagtaa taacgattgt ttttgctcca
      481 gtttttctta cggtttcagc aatatggatt gtttcttttg attgccctgt gtgagaaatc
      541 aaaacagcac agtctttttc cgtcaataaa gaagcttcca tcaattgaat atggtaatcc
      601 gtgctgtgag aagttgcgat aggggaacgc aaaaatttat gataagcgtc agttgctaca
      661 atctctgaac cgccaacacc gaaaaaatag acacgtttag attcattgat aatagtagcc
      721 gcttttttta gatccttttg ttgtaacaac ttttttgtgt catttaaagt tgaaatacta
      781 gactcgaaca ccttttgcgc catagtgagc tcagtatctg ttttctgaat attttcgtgg
      841 atagaaatag cagatagatc attttcttgg atcaacatgg ccattttaaa atctttaaat
      901 ccattataac caagtttttt tgtaaattga aaaaaagtag agtcagcaat gcccagttca
      961 cttgctaaat tgctaatagg actatgactt actttgcttg gattttctaa aatataatcg
     1021 gcgatagcct gttcttttgg gctgaagtcg ttgtacattg ttttgatttg tagtcttatg
     1081 gagacgctca tgagaattcc tcattttcat aataagatta tcattttttc cgttctcatt
     1141 aatgttaaag atacaaggag agtaggaaaa aatcaaacaa aaaatatttt tgtaaaatct
     1201 atttacaaaa aaattttttt gtataatgag ggtataagaa atggaggcgc tatcatggaa
     1261 attggtgtaa ttggtttggg aaaaatggga ttgaatttag ctttaaactt aaaagagcac
     1321 catcacaaag tacaaggact cgacatttca agtacagctg ttagtcaggc aagagaaaat
     1381 gacattcctg cttatcaaga gatctctgaa tttctagcta cttttgagca gaaaaaaatc
     1441 atctggttaa tgcttcctgc tggagagata accgaaaaca tgttaagaaa actttttcca
     1501 aaactcggac caggagacat tattattgaa ggcggaaact cttattataa agattcaatc
     1561 cgtcgagcag aagagtttca gaaaaatgaa atcggttact tggattgtgg gacttctgga
     1621 ggaatcgaag gagcacgaca tcaagcctgc ttaatgattg gaggagataa agaaactttt
     1681 ctatccgtgg aacaattatt caaagatgta gcaattgaaa gaggatatct gtatgctggt
     1741 gcatccggaa gcggacactt cttgaaaatg atccataatg gaatcgaata tggaatgatg
     1801 caagcaatcg gtgaaggatt ccaacttgta caaaaaagtg agtatgcttt tgacttggaa
     1861 caagtcgcac gtgtttggaa tcatggttcg gtgattcgcg gctggctgat ggaaatcatg
     1921 gaagatcagt taaacgaaca tcccaattta agtaattatc ggggaatcgt ttctgcatca
     1981 ggtgaagcaa aatggacggt tgaaacagcg ctagaaatgg ctgttccagt tccagtaatt
     2041 gcaactagtt tgttcatgag gaatctttca caggaagaag atagtttttc agcaaaagta
     2101 gtttcagcac taagaaatgg gtttggcgga catgaaatag ttgaaaaggg tgagtaaaat
     2161 gccagattat catatagcaa tcgttaattc cagcagtttc ggaagagtct ttccacacca
     2221 cttggaaaga ttaaagtcag ttggagacgt tcatttcttt actgttgatc aagagattcc
     2281 aggaaaagaa ctagcggagt tacttaaagg attcaatatc attattgcaa gtgttacgcc
     2341 attttttcga cgagattttt ttgaaaacaa agatgaactg ctattgattt cccgacatgg
     2401 tattggttac aacaatattg atttaaaagc agcaaaagaa caccagacaa tcgtatctat
     2461 catacctgct ttagttgaac gaaatgctgt agcagaaaat aatataacta atttgttgtc
     2521 catgcttcga cgcacgacac aagcacaagc aagagtcaag gacgatcgat gggaagatcg
     2581 ggcggagttc gtcggtagga cgctgtttaa caaaacagtt ggtgtgattg gtgcaggaaa
     2641 taccggaagc ggagtaatcg aaattttgcg taacggtttt cgctgtgatg ttctagctta
     2701 tgatccttac aaatcagatt tatacatcga aagctttggt gcaaaaaaag tagaacttca
     2761 agaattactg gaacaatcag atatcatttg tttatgtgcg aatttgacag aagagaacta
     2821 tcacatgatt tctgataaag aagtcgatca gatgaaagaa aatgtttatc tatctaatag
     2881 tgcaagaggc gctctagttg atgaagaagc gatgattaat ggtctgaaaa gcggaaagat
     2941 cgcaggatat gccacagatg ttttagaaga agaaccggga cgtcaagatc atccgtattt
     3001 agcgtttgaa caagtagtga ttacgccaca tatatcggcc tacacaaggg aatgtttaga
     3061 agcaatgggt gaaaaatgtg tcagagatgt cgaacagatc agtcagcacc ttcttcctga
     3121 acgttctgtc cagacaaaga gcaactatat aaaaggatga tgaagaaaat ggtgaacggt
     3181 ttaaaagtga aaacaggccc tcaattttat ttatatgaag aaggtggtat ttcaaaagtt
     3241 tctgatctct tgaaatcata cggcgcgaaa agagtactgg tcacccacgg aacggtatca
     3301 tgggaaaagg cgctacctaa attagtattt ttgaatgacg aaacgattca atttttttat
     3361 catcgttatt ctggagaatg tagttatgct gaagccagac gaatagctac aatcatcaaa
     3421 aaaatgaaat cgatttcgta gttggcgttg gcggaggaaa actgaccgat ctagtgggtt
     3481 atagtgccaa tcttgcaaga atcgatttcg gagtcatccc tactttagct agtaattgtg
     3541 ctccatggac acctcttgct gtcatgtata aggagaatgg acaagcagaa gggaaaacag
     3601 aacactttta cagacaagct gcttttttta taacagatcc ccaattagtg attgattccc
     3661 ctgttccata tttcattgcc gggttagcag atacactagc aaaatggtat gaatctgagc
     3721 tcatcttaga acaagatttt ttgcaagatg aatcattttt gaagctagca caagatactg
     3781 cgaagatctg caaagaagag attttgaatc attctgctaa agcaatccaa gatatggcac
     3841 aaagaaaact gacatcggag ttcaaacagc tttcagagat tatctttgct gttgctggtt
     3901 tagttggagg attaggagat aaatacgcaa gaaatgcggc agcacatgct atgcatgacg
     3961 ctatcagtaa atatattccg gaaagccatc gttttctaca tggagaaaaa gtagcgtatg
     4021 ggattttcta tcaactggca ctagaaggtc gctgggaaaa aatagacgag ttatatcctt
     4081 tgtatcggga attagaactg ccggtttctc tagaacaaat gggattattt ccgaaagata
     4141 aacagataat agataaaatg gctgaattta ttgattcgaa agagaaagtg catttgattc
     4201 ctatagaggt aacacagaaa tcactgatac agtcaatgat ggaactggaa ggttatatca
     4261 gaaagatctg aaggaggagg acacatggaa acattaagtg tagtacaaag tttgttgatt
     4321 gctttatggg tagccgcggt tatgtcccgc tggttaggtg gaggagccac gttgacctta
     4381 cgtttttctc ctttgatgac tggattagtc gtcgggttga tcatggggga agttgcacaa
     4441 gcgatgattg ttacagccgc tttgcaaatg atttatatgg ggggtttttc tccaggagga
     4501 tcaatgccag ctgaaccatc tattgcagca gctatcgcag taccagtcgc attattaggg
     4561 gatctgtcgc cagaagcagc gattgccgtt gcggtgccag tcggattgct cggaagttac
     4621 ttgtatcagt ttcgtttttt catcaatact tttttaggta aatatacaga caaagctgta
     4681 gaagatttga atacaaaagc tatggcccga tcaattattc tttatccaac ggttgcctca
     4741 tttttattat tcgttccgct tgttttcatt gctttatatt taggggcgcc attgatcgct
     4801 gatgtaatca aagctttaga gggaacagtc gttatccaca ttcttgaagt agtaggtgga
     4861 gggttagcgg caatcggtat cgcgacaact gtatacgtta tcggtcgaaa agattatctg
     4921 gtctttttct tccttgctta ttttatgagt gttgttttga aatctttaga tattacgatg
     4981 gttacttatg ccatttttgg gattattatc gctttgatct ttgtccagtc gcaaaaaggg
     5041 aaacaagtac agcctgccac aagcaataac aattcagcat cttttgatga cgaagatgac
     5101 tatgatgacg gattttaatt taggaggaat gggaaatggc tgaaataatg gataatcaaa
     5161 caggtgctcc tgaagaaatc acgaaaaaag atgtaacaaa agcctatctg agatggcatt
     5221 ttgcaaacga aattcctcac tcttttgaac gttacttggc gccatcacta ctatacggaa
     5281 tgatgccaat cttgaaaaaa ctttataaag atgaagactc tttaaaagca gcatataaac
     5341 ggcagttact gttcttcaac actcaattaa gctggggtgg cggtgtgatt actggcctaa
     5401 tgtcttctat ggaacagcaa agagcagaag aagagtatga agaaaaagag atcttgatgc
     5461 aggatgactt gatgtataac acgaaagcgg gattgatggg ggcactagca ggaatcggag
     5521 atgcaatcga ttcaggaacc gtccaatata tttttatcgc aatcgcggta ccttgggcac
     5581 aacagggaag tcctttaggc gcactttttc cttttatctg ctttgggttg tatcaggttt
     5641 tactaggggt atttttcgca agacaagctt tcactatggg ccgtaatgct acaggcctaa
     5701 tgcaaaacac tggtgttcag acagcgattg aaatgctatc agttttaggg ttgtttatga
     5761 tgggggtgct tgcgggaaac tatgtaaaag tagaatcttc attgaaattt gcgatatccg
     5821 gacgtgagtt tgttatccag gaaattcttg atcaaatcgt gccagggata ttacctttag
     5881 ctgtcgtaat gggtgtgtac ttgttctata cgaaaaaagg attgaaagtc acacaagcat
     5941 tattatggct gactggaata ttaattgtgc tagcaggaat cggtatttta taaaaagaag
     6001 ggaacgtgaa taagatgggt gtagtcaatt tagcgcgtgt ggatgagcgt ttgatccatg
     6061 ggcaagtaat ggtaacgtta tctcaaaaaa gtggagtgaa ttccattttt gttgtagacg
     6121 aagttgtagc aaaagataaa tttatgagag atctttacaa aaatgcagga agccgtacag
     6181 gacaaaaaac aattgtcatt acaccagaga aagctaaatt ctattgggat gaatatcaat
     6241 ttaaagaata caattgtatt ttgatcgcaa aaacagtcca aacgatttac gacttagtaa
     6301 aacacggaat ccccatggaa gaattgaata ttggagggat tgctcaaaaa aatcctgata
     6361 cagatctttt agtgacgaaa tctgtttatt taaataaatc agatgctgaa aaattgaaag
     6421 aaatgaatga acaatatggc gtaaagaata tttattttca agccactcca tctgcaccta
     6481 aaacaggatt aaaagatgta ttgaacaagt ttgatttgtc ataaacaaat gcttagaaaa
     6541 aagataaaga gggcgaaaaa atgaagaaga acgagctgtt tcgtgattgg gaatttcgtt
     6601 atcgctatat ttaccgtaag cgccgtacaa aaaagagtaa acagcgtttt ctttcagcat
     6661 tagtatcaga tatttacagt atgagaacag atgtgacagt cattgcttac gatacgctag
     6721 cctaccgatc aaaaaatatt tatgttggag atatcgaaaa agcagagaaa gttatctgta
     6781 cttattacga tacacccgtt catgcattag gaagttattt catgtttgat tggaaagacc
     6841 aaaggaaaaa aacaatctac tctatattat tgtccttcat tttgttgttc agtttaggtt
     6901 ggtggggaat gatgatatat aacaaaaatc ctcatcacgt attcgatctg ctatccgtat
     6961 aaacaggtat cactgtacta gcatttggga gctacttttt cttattggga aaagcagctc
     7021 gcggatggcc tagcagacaa acttttattc gaaatacttc atccatatta acgatgctag
     7081 aaatgatccg tacgattgat gatccgagtg tagcgtatgc gtttgttgat gaaggctgtt
     7141 atggagaaaa gggcttagac agcgtgagat cgggtatgaa aaaagaggct attctttttt
     7201 atctagactc tgttggggca gatacaccgc tacagttttc gggaaattat ttttcaaata
     7261 aagaacagtg gctgaaacaa gttgataagt tgaaagaaaa aaacgtcaat tatattttta
     7321 gtgcaagaaa aaagcaagcg cagttcttct atttgaccaa aacagattta cgagggaaaa
     7381 catttaattg gcaaaatgcc aatcagatta ttgctctttt tcgataagta aaaagtgcaa
     7441 aacaaggagt gagaagacta atgttggaaa tcatgatcgt tacacatggt aagttgagcg
     7501 aaggactgaa agatgcggcg gaagtaattg tcgggacatc gagccatatt gaaacaatgt
     7561 gcttagagtc tggggaggac atccaaacat tcggagagaa ggtcaaagaa aaattaacta
     7621 tgatcaacac agcagaaccc atgctagtat ttgttgatct aattagcgca agtccgtaca
     7681 atcaagcgct cttggcagtc aatgaactga ctgaagaaaa gaaagcagcc acttatgtca
     7741 tagggggcgt gaatctgcca atgttgttag aagcaatcaa tcatcagatg cttcacacac
     7801 cgatagaaga agcggtggaa caaattgtca aacaaggaaa agataattta actgtttggc
     7861 atgcttcagc ggaagaaaaa aaattagatg aggacgattt ttagaaggaa ggctgaaaag
     7921 atgaagtggg gatttagatg gtatggtgaa aaaggggatt cgatcccatt aaaatatgtt
     7981 cgccaaatac cgggaataaa tgggataatt ggaactttgc tgactaaaat gcctggagat
     8041 gtttgggaga tttcagaaat tgaggagttg aaagcatctg ttgagagcca aggactggaa
     8101 ctgcttggaa ttgaaagtgt agcgatccac gatgcaatca aggcagggac agatgaacga
     8161 gattactata ttgataacta tatacgaaca atcaaaaact tgtctcgatg tggggttcga
     8221 ttgatttgtt atagcttcaa accaattttt ggatgggcga aaactgattt gtttaatgaa
     8281 aacaacgatg gaagctattc tcttttatac gatcagaaaa tcgtcgatca gatggagcca
     8341 ggagaaatgt atcaattgat acacagccag tcaaagggat tcaaattgcc gggttgggaa
     8401 gaagaacggt tgaaaaaatt cgctcagctg gaaaaactgt atgaaggaat gaccgaagaa
     8461 gaattatttg aaaatctgac ttatttttta gaaaaagtga ttccagtgtg cgaaaaagca
     8521 gatgtaaaaa tggctatcca tccagatgat ccgccatggg aactttttgg ttttcctaga
     8581 gtgacgaaaa atcttggcga cttgaagaaa attctaaatg cagtagattc tccatataat
     8641 ggactaacgc tgtgcacagg atctttaggg gcaaatccgg aaaacgattt agttgaaatc
     8701 atccatgagg taggttcgcg catccatttt gtccacttca gaaacgtgga gtttttagga
     8761 gaaagaaagt tcaaagaaac ggcacatccc tctacagaag gctcccttga tatgtatcat
     8821 attatgaaag cgttggtgca agaagggttc gacggtccta tacgccctga tcatggtaga
     8881 accgtgtggg gtgaagtggc tatgcctgga tatggcctgt atgatcgtgc aatgggactc
     8941 acttacatgc aaggtctata tgaggcgata agtaaggaaa aaccactggg agagcattat
     9001 gaataaaatc aagctcttca tctttgatat ggatggcctt ttattagaaa ctggacgttt
     9061 agcttatcgt gcttatgtaa aatcagcaca aaagtatgat tatgaaatgc gtaaggaagt
     9121 ctattattta ctaactggcc aaacagaaat ggcgataaga gaacaaatgg gcattttgta
     9181 cggagaagat gttccatata tcaaatggcg agaagcaatc aatcaatata aagaaaaaat
     9241 cgtgaaagaa gataagagag tatatacgaa aaaaggagca gaagaaattt tatcttttgc
     9301 aaaagaaaga ggaatccata caatcgtggc ttcgtctaat acacgggtaa aagtagaaat
     9361 gtatttaaaa atggaaaatc tatatgcctt gtttgatcaa atcatttcag gggatgatgt
     9421 aaaaaaagga aagccggaac cagagatctt tttgaaagct tgttcaaaaa tgaatattcc
     9481 tccaagtgaa gcacttgtat ttgaagactc gattgcaggg atcgaagcag caaggcgagc
     9541 gggcattttg tcttttctca tcaaagatca tttgataggg ttgcccgatc ataatgggaa
     9601 gcataagttg aaagtagatg taagtttata tgaatccaat aaagcagact atgaatttta
     9661 tgacctgcat caagcaaaac gatttttaga aagtaaaggg ttgtatctct aataaagagg
     9721 actattttct agtcctcttt atttattgtc ctgcacgact gagggaacag atgaaaaaga
     9781 agtaattttt tattttgaag tatctacctt atttgtagtg aacaatttag aacaaacaca
     9841 aaacgcatct actgttatct accagtagat gcgtttaaag agaagaacga gaatcgtcca
     9901 tatgagggtt tccactcctt atgcagattt ttttaattct tgcactgctt gttcgatcgt
     9961 aacacctgtc gctacatgat tggtattttc tttgtcaaaa acgacaaata gattcaaatc
    10021 tgtatccaag gttactcgat attgtatatc tttgttatag aatgtcatag ccattctcct
    10081 ttctttaaat acttgctgaa aggcaacgaa cgcgttgata acgaaattcc atttataagt
    10141 ataccaccaa ttgaaaggtt ttcatagtta agaaatttta aagtcaaaag ttgcagataa
    10201 aagcaaataa acaagaaaac aaaaagtgaa tcaatcgcgg ttattcaact ttttcttcga
    10261 ttcgaagaca atagaagaca atgaatttta tatttcattg cattatcctt gtgttattag
    10321 aaaaagcatg atatattaaa tgagtaatct attttgaaac gtaatttgag cgatatatct
    10381 acatagataa aactcctttt gcctatcaaa tatttattgc aacgaaaaaa cgtacattga
    10441 tacgtatcag gaggaattta tttatgaata acggtacagt aaaatggttc aacgcagaca
    10501 aaggttttgg ctttatcact ggtgaagatg gaaatgatgt atttgcacat ttttcagcta
    10561 tccaaggtga cggcttcaaa actttagaag aaggccaagc agtgtcttac gatgttgaag
    10621 atggtcaacg tggccctcaa gcaacaaaca ttgttaaagc ttaataaaaa ttgactttta
    10681 accgtctttt cgaaagaaaa gacggttttt ttgtacgaaa aaatt
//
LOCUS       DARBDV010000092        10649 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000092 DARBDV010000000
VERSION     DARBDV010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.094"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            23..646
                     /locus_tag="SCM56_002366"
     CDS             23..646
                     /locus_tag="SCM56_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111110.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /locus_tag="SCM56_002367"
     CDS             1096..2145
                     /locus_tag="SCM56_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1111111.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="SCM56_002368"
     CDS             2142..3023
                     /locus_tag="SCM56_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1111112.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="SCM56_002369"
     CDS             3026..3796
                     /locus_tag="SCM56_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1111113.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="SCM56_002370"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="SCM56_002370"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEG1111114.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="SCM56_002371"
     CDS             5107..6270
                     /locus_tag="SCM56_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEG1111115.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="SCM56_002372"
     CDS             6387..6797
                     /locus_tag="SCM56_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG1111116.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="SCM56_002373"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="SCM56_002373"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEG1111117.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9370..9654
                     /locus_tag="SCM56_002374"
     CDS             9370..9654
                     /locus_tag="SCM56_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111118.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9814..10050
                     /locus_tag="SCM56_002375"
     CDS             9814..10050
                     /locus_tag="SCM56_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEG1111119.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10445
                     /locus_tag="SCM56_002376"
     CDS             10047..10445
                     /locus_tag="SCM56_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEG1111120.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3717 a         1773 c         2253 g         2906 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
    10141 atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc
    10201 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
    10261 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
    10321 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
    10381 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
    10441 aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa ggaatatgct
    10501 gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga aaatatggat
    10561 taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg aaatagtggt
    10621 ttagaactag tgggctgatt ttttatata
//
LOCUS       DARBDV010000093        10575 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000093 DARBDV010000000
VERSION     DARBDV010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.095"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            288..1067
                     /locus_tag="SCM56_002377"
     CDS             288..1067
                     /locus_tag="SCM56_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111121.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
     gene            1113..2057
                     /locus_tag="SCM56_002378"
     CDS             1113..2057
                     /locus_tag="SCM56_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEG1111122.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            2073..2852
                     /locus_tag="SCM56_002379"
     CDS             2073..2852
                     /locus_tag="SCM56_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HEG1111123.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            2864..3562
                     /locus_tag="SCM56_002380"
     CDS             2864..3562
                     /locus_tag="SCM56_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEG1111124.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3590..4354
                     /locus_tag="SCM56_002381"
     CDS             3590..4354
                     /locus_tag="SCM56_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEG1111125.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            4398..5849
                     /locus_tag="SCM56_002382"
     CDS             4398..5849
                     /locus_tag="SCM56_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEG1111126.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            5881..7017
                     /locus_tag="SCM56_002383"
     CDS             5881..7017
                     /locus_tag="SCM56_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEG1111127.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDVVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            7017..8123
                     /locus_tag="SCM56_002384"
     CDS             7017..8123
                     /locus_tag="SCM56_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEG1111128.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFKEFH
                     AIKNNSVLLIIGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLF
                     EGVPLVGIEAQFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRD
                     SERSILIKADYNIQTAYKILESKYYELFELIQRG"
     gene            8129..9151
                     /locus_tag="SCM56_002385"
     CDS             8129..9151
                     /locus_tag="SCM56_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HEG1111129.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVLAQRINHEIAQTF
                     DKIPSNNPLCDELIKNLNNIYDEKVWNLLKKDTLLFKLTWKQSFKKQKDDKVTFYGML
                     VENKLL"
     gene            9173..10228
                     /locus_tag="SCM56_002386"
     CDS             9173..10228
                     /locus_tag="SCM56_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEG1111130.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEYTDYCY
                     RKEEQESITKSLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAIDYCRLYVGLVD
                     DANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQQKFFHKMLECKCYTILLLLSR
                     IRKIIKNMTRKKLFIIL"
BASE COUNT         3878 a         1470 c         2156 g         3071 t
ORIGIN      
        1 ttttgttatt ctattttttg tgtaactttt aaaaacacct atgaaaatta tggaaataaa
       61 aatgaaatcg attaattttt atcttattaa tgttttattt acttttaaat cgtaaattgc
      121 cctagtcaag aaaacagttt gtggtatgat tatataggga aaacttgttt acaaatattt
      181 ctgtgaatat attagtttaa ttatgttttt ttaaataaaa agctgtcatt tttatgtaag
      241 tgaattatat cttaatttat ctatcatcaa tacttaggag tgaatctatg gaaaagccat
      301 tcgtcaagtc attgactgct gtggcggtag gtgcaggtgc tattgcgatc tgtttgtttg
      361 gataccatat caataatcag cggcaacatc agcaacggat caattatgca gaatctgcca
      421 tcaccaatca aaaagacacc ttgacttctt tatcaaaaga agtagacaag ctgtatagta
      481 caaaagaaaa aatttttcta aaccctgaaa taacagaaga aacaatcaca agtttgtcaa
      541 ataagctatc ctctgtcaaa ctttcagcag atgattacga catcaaagaa tcagaattgc
      601 ctaaagaagc tgttgcaatc caagatgaga agaaagcaat cgtgacacag ttagatagtg
      661 cagaatcaaa actgaagatc cagacagctg tcaacgattt atttactaaa aatgtatcaa
      721 actggcagca agctgtagac gatgtcatca tcaaagaaaa ggtgacaagt gcagatgtcg
      781 ctcgtgtacg tgaaaatatg agctttttta aagatagtcc ttggaaaacg gttgttatgc
      841 aatatctagg attcgctgat attcagatcg cgcaagtcac gcaactagat cagttattcg
      901 atacgatgtt aaaggatggt caagtgactg ccaccgctac ttacgatcaa tatttgacgg
      961 tgctcagtca aatagagcag atccggaatg aaaaaataag cgcagcatac gcaacaaaag
     1021 cacaaactgt tgctgagcaa atgggctata gcaatataag ctattgacag ttaaaacgtt
     1081 gattcaagca aggttagcaa acggaggaaa ctatggcaag acgaagaaga aaatcaagag
     1141 ggaaaaaaat atttctgatt attttagcag ttcttatcgt attagtatta gcaattgtag
     1201 gagtaggagc aaaattatat ctggatctct cacattctat tgagaaaact tacgaatcag
     1261 tggaacgaac ccaaggagaa cgaacgaaac cagttgatct ggacaaacaa gaatctttct
     1321 ctgttctatt gatggggatt gatactggag atttaggtcg ggtcgatcaa ggaagatcag
     1381 atacgatgat ggttgccaca gtcagtcctg aagacaaaca gacaacgatc gtcagcattc
     1441 ctcgtgatac gtatgtcgac atcgtaggtc gtgacacaaa agacaagatc aaccacgctt
     1501 acgcattcgg cggagctgcc atggcgatgg ataccgtaca aaaatatgta gacatcccaa
     1561 tcgatcacta tgtctctatc aatatgaaag ggctaaagga actcgttgat gcagttggtg
     1621 gtatcgaagt caataatgac ctcactttct cacaagatgg ctatgacttt ccaatcggta
     1681 aaattacatt gaacggggat caggcattat cttattctcg tatgcgttat gaagacccaa
     1741 atggtgatta cggtagacaa gggcgccaaa gaaagatcgt agaaggcgta gcgaaaaaag
     1801 tattgagtct aaacggtgtc agcaactatc aatccgtttt gaatgctcta gagtcgaata
     1861 tgaaaacaga catgaacttt gatgaaatga aaaaaattgc tttagattat cgggaggcat
     1921 ttggtacgat taagcaagat cagctgcaag gagaaggctt catgcaagat aatatctcct
     1981 atcaaaaagt cagtgatcaa gaacttgatc gtgtgaaaaa agaattaaaa gatcaaatga
     2041 atctagagaa taaataggaa agtggagaaa aaatggaaga gacaatcagc ttgaaagaaa
     2101 tttttgaaac attacgcaaa cacttggtaa taatcatcat ctcaatgttt gctggcttgg
     2161 ccatctcagg aatcgtaaca tttttcatct tgacaccaaa atacagttca caagcacaat
     2221 tgatcgtgac actgccacaa tcagacactg caaatgtcaa tgatgtcaat acgaacctgc
     2281 aaatgatcaa tacgtataaa gatatgatcg tcagtgattt agtgctcaat gaagtcaaag
     2341 atcgtttaga aacagaagac aatgtgaaaa tgactgctga acaaatcaaa gatgccatct
     2401 cagttaatca ggcagaaaat tcacaaatgt tctcgattca ggcaataagt acgaatgcag
     2461 tggctgctca acagatcgct aacacaactt cacaagtttt ccaagagaac gcaaaagatg
     2521 tgatgaatgt tgataaaatc tcgatcattt caggtgcagt tgccaatacg acacctgttt
     2581 cgccaaataa taaattgaat ttaatgactg gcttaatgtt aggaatgatg gtaggtgtag
     2641 gtctggcatt cttattggaa ttactcgatc ggacagtgaa agatgaaaaa tatgtgacag
     2701 atacattagg attcccaatc ttaggaacag ttccagaaat gtcagcgaaa gaattgaatg
     2761 ctggtatccg taaaaaagga ccagctcaga cacaaccaaa agctacaaac gataaaacag
     2821 cgccatcacg cagaagccgc tcaagagtct aggaggaaag aaaatggcac gaacacagaa
     2881 acaaaaagca gcccaaactc atgcagtcag tttggtcaca ctcaccaatc cctcttcacc
     2941 aatcgctgaa caatatcgta cgattcggac gaatatccag tttgcctccg cagctggtca
     3001 gcaaatcaaa acgattgttg tgacttcatc aggacctgga gaagggaaat cgaccactgc
     3061 agcaaacatt gccgttgttt ttgccaaatc tggtcaacgg gtgctgctag tcgatgcaga
     3121 cttacgtaag cccgtcatct ataaaacgtt caagttgaac aacgcaagcg gattaagtac
     3181 tgttcttagt tcatcaggaa gtgtagcaga tgagatccaa cgaacaccag tcgacaattt
     3241 gtcgattctg ccaagtggac ctaaaccacc aaatccatca gaactactaa gttcgccaag
     3301 aatggatcag attttagcag aagcaagaca actatttgat gtagtgatct tcgacatgcc
     3361 gccagtcgtt gccgtaacag atgcacagat catgtcttca aaaacggatg gcaccttatt
     3421 ggtcgtacgg gaaaatacgt cacgaaaaga atcattgact aaagcaaaag aactgttaga
     3481 tatggtacaa gcacgagtct taggtgtcgt atacaacgga gcagaacaca gcaaagatgc
     3541 tggttattac tacggaaatt aaggaataag aagagaggag acaagagaaa tgatcgatct
     3601 acattgccat atcctaccag gaatcgatga cggggcagaa aacttagaag caagtattgc
     3661 aatggctgaa aaagcgattc agcaaggcat tacgcatatt ttatgcacac cacaccacaa
     3721 taatggaaaa tacagcaatg aaaaatcaca agtcatttct ctagtcgctt cgttacaagc
     3781 cgaattagag aaaagacagt tgcctttgac gttattggaa ggacaagaag tacgtattac
     3841 cggaacgctc atcgaagaca tccaccgaga tgaaatttta tttacagatt tagatgatac
     3901 atatcttctg atcgaatttc cgacactgga agtgccgtta tatgcagaac ggcttttttt
     3961 tgagttgtta gaactaggaa agataccagt gatcgtccat ccagaacgca acgcgcattt
     4021 tcgagaagac ccaaatcatt tgcttccgtt cctagatatg ggatgtttgg cccaacttac
     4081 tgcgccaagt tatgttggtg tatttggcaa agatatccag aaaacagcca aaacaatggt
     4141 caaacataat ctcgtgcaga tggctgcttc tgatgcacac ggggtctcta agcgcacctt
     4201 ttacttaaaa gagtgctatg agcagattgc caaggagttt ggtaaggaaa aagtagtaca
     4261 gatgaaacaa gtcgcaaagg atctgatcaa cggggatgag atcaattatc caatctatga
     4321 agaggtaaaa aagaagaagt ttggtttatt ttagttcttt attcgtagat aaaaaggaaa
     4381 tgaggagaga gtgagagatg tatagaaaaa actcaagtgg ttggtctaaa catattgatt
     4441 ttattttatt agacttattt tgtgtccagc ttgcttttta tatatcatat gtaatgcgac
     4501 agggtgactg gaatccgtat gtgattccta tttatcgcca catggcaatt tttatagaac
     4561 ttgagaatct tacgattata tttttatttg aggcatataa aaatgtttta aaaagaggat
     4621 attataaaga ggctgtagct tctataaagc aatcttttat gctgttactt gtgtgcagtt
     4681 tataccttat tacaatgcag gatggaaatg attattccag agtttcttta tttataatgg
     4741 gggtggtgta tggattatta acatatataa ttcgtatact ttggaaaggg ttgttgcatc
     4801 accgcatgaa aaacggtgaa gatgtatcgt tagttattgt aacatctcaa aagattgcaa
     4861 aaaatgtagt tcgtaatctt aaagagaaga attatggcat gtataaactt agtggtgtga
     4921 ttgtagtaga cagagatatg tatggaacta ccatcggagg tgtcccagta gttgcaaatg
     4981 ctgataatgc agcggaatat cttttacaga attgggtaga tgaagtattc atcaatgttg
     5041 acgaaagtgt tccatatccc aaagaattaa tcgccagatg ttcggaaatg ggacttgctg
     5101 tacatatgaa tcttacaaaa gtgtctgata gtacacgtgg aaaacaattt attggccgag
     5161 ttggtgatta tactgttatt acatctagta ttaatgttat gacgatgagg caagcattta
     5221 taaagagaac gattgacatt cttgctggaa ttattggatg tattgcaaca ggtgttattt
     5281 ttatcttcgt agcaccagtt atttacatca aatcaccagg gccgattttc tttgcacaag
     5341 aacgtattgg tcaaaatggt aagacattta agatgtataa gttccgtagt atgtatatgg
     5401 atgcagaaga acgcaaagca gagttgatga aagaaaataa gatgagtaat aatctcatgt
     5461 ttaagatgga ttttgaccca agaatcattg gcaataaaat acttcctgat ggaacaaaga
     5521 agacaggaat tggacaattt attcgatcta catcattgga tgagtttcca caattttata
     5581 atgtcctaat gggaaaaatg agcctggtgg ggtttagacc ttgcttgaag tcagaatatg
     5641 agaagtatga ttttcatcat cgtgcgagaa ttgcgatgaa acctgggatt actggaatgt
     5701 ggcaggtgag tggtagaagt gatatcacag acttcgaaga ggttgtaaaa cttgatgcag
     5761 aatatatcag gaattggagt atgggattag attttagaat tttgtttagg acagtcaaaa
     5821 ctgtgcttca tagaaacggt tccatgtaag ataaagatac tatagaagga gtaaaacgtg
     5881 atggcaagaa aaaaattaaa aatctgtatg ctaggtcata aaagaattcc atcaagagaa
     5941 ggtggaattg agatagtggt agaagaattg gcaacgcgaa tggttgcact tgggcatgaa
     6001 gtgacttgct ataatcgaag tggtcatcat gttagcggaa aggaatttga ccagcataaa
     6061 aataaagaat ataaaggtgt aaaattaaag actattttta cattaaatat aaaaggaatt
     6121 gcagcaatga gttcttcagt atttggtgga attagagcag cattcggaaa atatgatgtt
     6181 gttcattttc atgcagaagg accatgtgct atgttgtggt tgccaaaact ttttgggaaa
     6241 agatgtgttg caacaattca tggtctagat catcaaagag aaaaatggaa caagcttgct
     6301 agtacataca tcatgttagg ggaaaagtgt gctgtaaaat ttgcagatga aataattgta
     6361 cttagtgaaa gcgtccaaaa ttattttgaa gatatatatg gaagaaaaac aaggtttata
     6421 ccaaatggcg ttaaaaaaat tgaaataaaa tcagcaggat tgattacaga aaagtatgga
     6481 ttaacaaaag attcttatat tttgtttttg gggcgattgg ttcccgaaaa aggaattcgg
     6541 tatctaattg aggcatttaa agatgttcag acagataaaa aacttataat agccggagga
     6601 agctctgata cagatgaatt tgcaaatgaa ctaaaggaat tagcaaaagg tgatgagcga
     6661 attatattta ctggatttgt acaaggacag gaattagaag aattatatag taatgcgtat
     6721 atctatacac ttccaagtga tttagaagga atgccattaa gtttgttaga agcaatgagt
     6781 tatgggaact gttgtatagt ttcaaatatc tcagagtgta cggaagtagt agaagacaag
     6841 gctatgattt ttaagaaatc ggatgtaagt gatttaaaga taagattaga agaggcatgc
     6901 aatcaatcgg aaatggttaa agttttgaag aatcaagcta ctgagtttat ttgtagtaaa
     6961 tataattggg ataaaatcgt acaagaaaca ttaaatttgt ataggggaaa tatataatga
     7021 ttcgaatatt acagtgtgtg aatgatatgc atcgtgcagg gttggagacg atgctaatga
     7081 actattatag gaatatagat cgaactaaaa ttcagtttga ttttttgaca catcgtccat
     7141 ataaaagtga ttatgatgag gaaataataa gtcttggagg aaaggtctat tatgcaccga
     7201 gactttatcc acaaaattat cctaaatatt ttaaatggat ggaacaattt tttaaggatc
     7261 atccagaata tgagattgtt cattctcata ttgatgcgat gagttattta cctttaaaag
     7321 ctgcaaaaaa agctgggatt ccgatacgga tagcacatag ccataataca tcgatagata
     7381 gagattttaa atatctttta aagcaatatt atcgatgtaa gttaacgtct gttttgacac
     7441 atgaattttc atgtggaaaa gtagcaggtg attttttgtt tcgaaatgat aaattcactt
     7501 tgattccaaa tgcagtggat gctaaaaaat tttattatga tgaaagtatt agaaatgatg
     7561 tacgaatgaa attaggaatt tcaagtcaga cgtttgtaat gggacatatt ggaagaattt
     7621 catattctaa aaatcatcgt tttttgattg aaatttttaa agaatttcac gctattaaga
     7681 ataattccgt attattaatt attggaactg gtgatatgga agaagaaata aaaaattatg
     7741 caaagaaaag tggcatagat gatgatatta aatttttagg aaatagaaat gatatagaaa
     7801 aattttatca agcatttgac gtattaatgt tgccatcatt atttgaaggg gttccgttag
     7861 tgggtattga agctcaattt gcagatttac cgtgtttctt ttcggagaaa gttcctacag
     7921 aagttgcttt cagtgataag accaatttca taagtttaaa tcaatcggca aaagaatggg
     7981 cgagagagat tgcaaatgta gatatctctc atcgagattc agaacgttcg attttaataa
     8041 aggcagatta taatattcaa acagcataca aaatcttaga atcaaaatat tatgagttat
     8101 ttgaattaat acaaagaggt taaagaatat gacacagaaa cattcaagta taaaaagaat
     8161 tatacatcgc atatccgaag aattgaatgc aacaagacga attgcaaaga taacatcttg
     8221 gaaggaagca tttgccacgt tacaagctaa aattgatatt caagtcatga atcgtaatgg
     8281 atataatgaa agtaaaaaac agaaggaaca tttattaaaa aaacatgagg taatgctgaa
     8341 gtattatgaa aagacttttt cagatttttt gaaaacatat gattatagtc aaaatcaaat
     8401 aattaatcaa tcaaaatcac catattctaa ctgcatttgg gtttgttggt ggcagggatt
     8461 ggatcaatcg ccggaaattg taaaagcttg tgtaaaatca ataaaacgaa atgcagggaa
     8521 acatactgtt ataattttaa cagaagataa ttatagacaa tatgttgata taccaaaatg
     8581 ggtggaagaa aagaaaaaca aaggaatcat tacgagaaca aattattctg atttattaag
     8641 attatctttg ctagcaaaac atggaggttt gtggttagac gccacatttt attgtacaaa
     8701 accaatacca gatgagtatt ttgatatcga tatttggtct attaggaggc ctgattatgc
     8761 tcacgcatcc gtagcatcag gatattttgc gggttattca ttagcatgta atcaagagtc
     8821 tagatggatg tttgctataa ttagagactt ttttttaaat tattggaaaa ataatgatat
     8881 gatggtggat tatttgatga tagattatat gattgtttta gcacagagaa taaatcatga
     8941 aatagcacag acatttgata aaatacctag taacaatcca ttatgtgacg aattgattaa
     9001 aaatttaaat aatatatatg atgaaaaagt atggaactta ttaaagaaag atactttact
     9061 gtttaagttg acatggaaac aatcttttaa aaagcaaaaa gatgataaag ttacttttta
     9121 tgggatgttg gtagaaaaca aattattata aatattagag gtataaaaag ttatgaaaca
     9181 caaggtaagt gtgatagtac caatttacaa tgttgaaaaa tatttaagaa aatgtgtgga
     9241 tagtttattg agtcagacat taaaagagat agaaatcgta ttagttgatg atggttcgcc
     9301 agatgcttcg ggggaaatag cagatgagta tcaaaaaaaa tattctaatg taaaaacaat
     9361 acatagagaa aatggggggt taggaccggc aagaaataca ggaattgaga atgcgacagg
     9421 agaatatgtg gcttttcttg attcagatga ttgggtagaa agtgatatgt atgaaaaatt
     9481 atacttagtt gcgtccaaag aaaatgctga tatagtagtt agtggacatt gtgattttgc
     9541 gaatgacaaa gctatggtga aaaagcaaca tcctttgagt gggaatgtat atgatacgcc
     9601 taatagtatt aaaaatgtac gaaaaaattt atttggacat agtccaaaag atcaagaagt
     9661 agaggccttc ccgatgtcag tatgtatgtc tttgtataat aaaaatctgc tagataaata
     9721 tagactaaga tttgaaaaaa ttttatctga agatacaatt tttaacttga atgcatatga
     9781 tgtcgcaaat aaaattgctt ttacagaata tacagattat tgttatagaa aagaagaaca
     9841 agagtctatt acaaaaagtt tatctccaaa taagcagacg gaatttaaag aatttttaat
     9901 aactctaaaa gctatagcat cgaaagaaga tttagaatgt caaatcagag caaaacgtat
     9961 ggcaattgat tattgtaggc tttatgtggg cttagttgat gatgcaaatt tgggaattaa
    10021 agagaaaaga aagtatataa aaaaatttat tgaagatgag ggattaattc aatgttggaa
    10081 gggatatcct acaaatacgc taccatttca acaaaaattt tttcataaaa tgcttgaatg
    10141 taagtgttat acaatattgt tgttgttaag tagaattaga aagataatta aaaatatgac
    10201 cagaaaaaaa ttgtttataa tattataaac taaaagtata tcaaataata gaaattaaca
    10261 agttacaaaa aaatattata aagaataata ttttctaaaa agtaaactat agtaatgtgg
    10321 attatcacct tccaacagta tataaaatga gcgtaaaaac ggctattttt gccttaacaa
    10381 cgattttttt gtatacaaat acgatatgct cattaattat tggaatttgt agtgctgttt
    10441 aaattaatga aggtgtaatt atatgagtga tcattttgaa aaagtttgtt gagaagatgt
    10501 gcttctagtc atgtgatggg agaaaataat ttaactgtta gagtgaatag ttaaattatt
    10561 ctggttatat gaaaa
//
LOCUS       DARBDV010000094        10489 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000094 DARBDV010000000
VERSION     DARBDV010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.096"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(14..679)
                     /locus_tag="SCM56_002387"
     CDS             complement(14..679)
                     /locus_tag="SCM56_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1111131.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            655..828
                     /locus_tag="SCM56_002388"
     CDS             655..828
                     /locus_tag="SCM56_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111132.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(873..1610)
                     /locus_tag="SCM56_002389"
     CDS             complement(873..1610)
                     /locus_tag="SCM56_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEG1111133.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(1612..3114)
                     /locus_tag="SCM56_002390"
     CDS             complement(1612..3114)
                     /locus_tag="SCM56_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEG1111134.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            3373..5367
                     /gene="uvrB"
                     /locus_tag="SCM56_002391"
     CDS             3373..5367
                     /gene="uvrB"
                     /locus_tag="SCM56_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEG1111135.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            5377..8193
                     /gene="uvrA"
                     /locus_tag="SCM56_002392"
     CDS             5377..8193
                     /gene="uvrA"
                     /locus_tag="SCM56_002392"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEG1111136.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            8456..9721
                     /locus_tag="SCM56_002393"
     CDS             8456..9721
                     /locus_tag="SCM56_002393"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HEG1111137.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            9725..10456
                     /locus_tag="SCM56_002394"
     CDS             9725..10456
                     /locus_tag="SCM56_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HEG1111138.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
BASE COUNT         3363 a         1838 c         2241 g         3047 t
ORIGIN      
        1 ttttttgtag ttgtcatgtt tgtaccaaca aaaaagccag ctttagaata tgataatcgt
       61 catagtttct aggggtataa ccagtgagag aatggaactt gttcaatcga tattgcaagg
      121 tgtttttatg aataaataat tcttctgagc aatttttgat gcttccgtta tttttactga
      181 agcatgccaa agtcttcttg aacaaatgta cttcttcttc tgtcaggctt ttcaaaatac
      241 gttttacata taatgttcgt tgatctgacg ggattgctgg taaaacgatc ccgaggtcta
      301 aatcttcaaa aaagcggatc tcgttcacag attgatggaa agctatccat tcgcaaactt
      361 gtttggcatg aaggaaagat tgcggtgtct cttgctctgt ataacaaagc gacccaattc
      421 caaagtttag ctgtaactga tacattttat taaactttgt ctggatttta tcaagcaatg
      481 atagcaatga ggctctggaa atccgattga ctaggataat gatttgttgt ctatgtacag
      541 tgagcaaagc ctgtccgtaa taagagaaga ccctttcgag caactccaat aaaggattga
      601 tccgttcttg acacaattgc tgattgtcag aaagttgtcc cacaactaca taatatggag
      661 aagaaaaatc aagctcaatt aagtgagggt ggtatcgact atgtacttga tatcatcaat
      721 gaaccgatgg tactttctta tgcgatggaa caggtagagc gattgcttgt ttgtgcagga
      781 aaacaagcga tgcggctgat acgattaggt gagaaaataa gaagataaaa atacacggtc
      841 ccttcatggc gaccgtgcat ttttggtttt tattaaatat ttaatacttt attcaagaaa
      901 tcttttgtcc gttcattttg cggattttca aagatttgtt gaggcgttcc atcttctaaa
      961 aagtttccgt cgtcaatgaa catgacgcga ttggccactt ctttagcgaa ccccatttcg
     1021 tgcgtaacga taaccatcgt cataccttgt tctgctaact ttttcatgac gttcaagaca
     1081 tcaccgacca tttcaggatc gagtgcagaa gtaggctcat caaaaagcat gatgtcaggg
     1141 ttcatcgcta gagcacgagc aatagccaca cgctgttttt gtccaccaga aagcatttca
     1201 ggatacatat cttttttatc ttctagacca actgtctgca gtaatttgat tgcttttttc
     1261 tctgcttctt ctctagattc tttcttcagg tccatcggtg ctaaaatgat gttttccaaa
     1321 atagaaagat gagggaatag gttaaagtgt tggaatacca ttccgatatg ttgtctcacc
     1381 aagttgatat ctgtgctttt atccatcaag tgggcgccgt caataataat ttcaccgtca
     1441 gaaggttctt ctaaacgatt caagcaacgc aagaatgtac tttttcctga acctgaaggg
     1501 ccaatgatac agacgacatc tccttcgttg atggatacat tgatgtcgtt taatactgtg
     1561 ttatcgccgt attttttgac taaatgctca actaagattt tttctgccat cttacttcac
     1621 cttcttttct aaaacttttg ctaattttgt caatgctgtg atcaaaatca agtacatgat
     1681 cgcaatgatc aagtaaacat acgtactttg tgttgtacgt gcaacaatga tcttaccagt
     1741 ttgcagcaat tcgactacac cgattgcgga aatgatcgtt gtgtctttta atgagataac
     1801 gaactgattg acgaatgatg ggatcatgat gcgtatcgct tgcggcaaaa tgattttttg
     1861 cattgtccga ttataagaaa gacctaaact tctggaagct tccatttgtc cgacaggaac
     1921 ggcgttgatc ccgcctcgaa caatctctgc gatataagca cttgcgttaa gagtcaaggt
     1981 gataacacca gcaaggaagt caggaatcgt aaatccaacg ataccaggca acccaaagaa
     2041 aataaagaac gcaagaacca tcattggaat tcctcggatg atatcgacat aaatactagc
     2101 aattgtgcga agagcgcgta ttggtgctac actgaataat ccaaaaatca caccgattac
     2161 tatagccaaa gcaaaggaga tcaaagttag gagaattgtt ctccaaagtc ctttcaacaa
     2221 tgcttgatag ttgttttgga taagtccaag aaatgttgat tcatcagcag tggaagactc
     2281 actggaagtt tctgaaccat ccgctacata ttctttaata atcttatcgt attcgcctgt
     2341 tctttttaat tctttcaatc cttcattgaa catattaaga agctcaggat tttgtccttt
     2401 tttaacggca aatgcatatt ttccgccaga ttcttttttg atcggtgttt ccaattcttg
     2461 tccttgtttg atcgcataac cgattaccgg ataatcatcc atcattgcat cgattgcgcc
     2521 aacttttaac ccgtcgtaca attgatcagc agcatcaaaa tatttgatag aatagccata
     2581 ttcagattca tggtcgctta aaaattcagc actttcggta ccaacttttg ctccgactgt
     2641 ttttcctttc aaatcttcat aagacttgat cgaagtatct ccttttttga cagctaattg
     2701 gatcccgcta tcaaagtaag aatcagaaaa atcatagctt ttctttcgtt catctgttac
     2761 agtcattccg gcaatcattc cgtctgcttg tcctgattcc aaagcttgca aagcagcaga
     2821 gaaaccacgg tagtccattc ggatgttaaa tccttgcagt ttggcgatgt tttccatcaa
     2881 ttttacatcg atgccttcgt actcgccctt tgcgttttga aattcaaatg gagcaaaagc
     2941 agagtcactg gcaattacgt atacgtcttt tttgggctct attttttgca ttgtctcgct
     3001 tgtgcttgac tctgccgttg tagtgttgtc agcttgtgca acatgaccaa acgcaaatgt
     3061 cacaagtcct gtcattaaga aaacaaatga gtgaaataaa tgctttcttt tcataatcaa
     3121 cctcctaaat ttccaatatt ttattattat aggcgaaaag tgctgaatca gacaagactg
     3181 taagctcgaa aaaatgaaaa aaatgcgagt ttctaacatt ttatgttata aaacataaca
     3241 tgtataataa gataaaaaag tggcgaaagt atgttcggtg tggtataatt ttttagtaag
     3301 gaatgccttt gctttccttt tctttttatt ggtcgtaaaa tgaaaaggaa tgaatgaaaa
     3361 ggaggacgaa gaatgattga gagagatacc tcccgacact ttgagcttgt ctcgaaatac
     3421 caaccggctg gtgatcagcc tgaagcgatt aaccaattag tggatggcgt agtcggcggg
     3481 aaaaaagcac aaatcttatt aggtgcgact ggaacaggga aaacatatac gatttccaat
     3541 cttattgaga aagtgaataa accaacactg attattgccc ataacaaaac attggctggt
     3601 cagctgtacg gggaattcaa ggaatttttc cctaacaatg ctgttgaata ttttgtttct
     3661 tattatgatt attatcagcc tgaagcttat gtgccttcaa gcgatacgta tatcgaaaaa
     3721 gattcaagtg taaatgatga gatcgacaaa ttacgacatt cggctactag ttcattatta
     3781 gaaagaaatg atgtgatcgt cattgcatcc gtttcatgta tttttggatt aggctcgcct
     3841 tttgagtacc aaaaacaagt agtttcgatt cgacagggag cagaactaga tcgcaatcag
     3901 ttgattcgtg acttagtcag tatccaattc gaaagaaatg atatagattt tcagcgcggt
     3961 cgttttcgag tgcgcggtga tgtcgtggag atttttccgg cttcaagaga tgaacgtgcc
     4021 ttacgtgtgg agttttttgg agatgaggtc gaacgtatcc gagaagtcaa tgcactgaca
     4081 ggtgaagttt taggagaaac ggaacacgta gcaatcttcc cggcgactca ctttgtgacg
     4141 aatgatgaac atatggagca tgccgtagcg aatatcaaag cggaattaga acagcgttta
     4201 actgtcttgc gaaatgaaaa caaacttctg gaagctcaac gtttagaaca gcgaaccaat
     4261 tacgatattg aaatgatgtt agaaatgggc tacacgtcgg gaattgaaaa ttattctcgg
     4321 catatggacg gacgtaaaga aggtgaaccg ccttatacgc tattggattt ctttcctgaa
     4381 gattttttga tcgtggcgga cgaatcgcac gtaaccatgc ctcaaatcag aggaatgtat
     4441 aatggtgacc gagcgagaaa acagatgcta gtagattacg gtttccgttt gccatctgct
     4501 ttagacaatc ggccacttcg gttagaagag tttgaaaaac atgtcaatca gatcatttat
     4561 gtctctgcga cgccaggacc atatgaacat gaacaaacag ataccgttat ccaacaaatc
     4621 atccgtccga cagggctgct tgacccagtg atcgaagttc gtccaatcat gggacaaatc
     4681 gatgatctag taggcgaaat aaatgaacga gtcgaaaaag accagcgggt gtttgtaacg
     4741 acattgacga aaaaaatggc agaagacctc acagattatt tcaaagaatt agggatcaaa
     4801 gtcaaatatt tgcatagtga tatcaaaacg ctggaacgaa cagaaatcat tcgagatctt
     4861 cgattaggtg aatttgatgt gttagttgga atcaatttgc ttcgtgaagg attagacgta
     4921 cctgaagttt cccttgttgc tatcttggat gcggataaag aaggcttttt acgtagtgaa
     4981 cgttccttag tccaaacgat tggacgagct gctcggaatg aagaaggtaa agtaatcatg
     5041 tacgcagaca aagtaacaga ttctatgcgt ctagcgatgg atgaaacgtc aagacgtcgt
     5101 acgattcagc agaagtacaa tgaagaacat gggatcgttc caaaaacgat cattaaagaa
     5161 atccgagatt tgatttctat tacgaaagaa tcggaagatg atacaaaaga agcggtacaa
     5221 gtctcgtacg aagaaatgac gaaggaagaa aaagacacgt tattgatgaa attagaaaaa
     5281 gaaatgaaag atgcggcaaa agctttggat tttgaaacag ccgcgaatgt gcgagatatg
     5341 attttagaat taaaagcttc aaagtaggag gacaacatgg ctaacgataa aattacgatt
     5401 catggtgcac gtgcccataa cttgaaaaat atagatgtaa cgattccacg agataaattc
     5461 gtggtcgtta caggtttgtc cggttctgga aaaagttccc tagcatttga tacgctgtac
     5521 gctgaagggc agcggagata cgtagagagc ctttctgctt atgctcgtca gtttttagga
     5581 caaatggata agccggacgt agatagcatc gacggattga gtccggcgat ttctattgat
     5641 cagaaaacaa caagtaaaaa tcctcggtcg acagtaggta ctgtgacgga aatcaatgac
     5701 tatttacgct tgttgtttgc acgtgtagga catccgattt gtcccaacga tcatatcgag
     5761 attaccagtc aatcagtaga tcaaatggca gatcaagtaa tggctttgcc tgagcgcaca
     5821 aagatccaag tgttggcacc gatcgtggta aagaaaaaag gacaacataa aaaaatattc
     5881 gaacgaatcc aaaaagaggg atacgtccgc gttcgagtcg atggtgagat ctatgattta
     5941 agtgaagtgc ctgagctgga gaaaaacaaa aaacatgata tcgcgatcgt catcgaccgg
     6001 atcattgtga aagaagggat tcgttctcgc ctatttgatt ctttagaggc ggccctgcga
     6061 ttagcagacg gctatgctct ggttgacgtg atcggtgaag aagaaatgtt gtttagcgaa
     6121 cactatgcct gtccttattg cggatttact gttggcgaac tagaaccaag actgttctca
     6181 ttcaatgcgc cttttggagc ttgtccagac tgtgatggtt taggtgtcaa attagaagtc
     6241 gatcaagatt tagtgattcc agatagaacg aagacactta gtcaaggtgc tatcgttcca
     6301 tggaatccaa tcagctctca gtattatcct caaatgttag cgcaggcttg tgaaagtttc
     6361 ggtatcgatt tagatacacc attcgaagaa ttaccggaag agcatcagga gatcatcttg
     6421 aatgggacag atgatctttt ccatttccat tatgaaaatg attttggtgg cgttcgcgat
     6481 gtagaaacga ctttcgaagg ggtattgaag aatatcgagc gtcgttatca tgatacgaac
     6541 agtgatttca cgcgtgagca aatgcgtctt tatatgacag aattaacttg tcagacatgt
     6601 aaaggttatc gattgaatcc ccaagctcta tctgttcaaa tcaatggaac gaatatcggg
     6661 caaacaaatg agttgtctat tcaaaaagca gtagatttct tctcaaactt aacgttatct
     6721 gaacaagaga aagtaattgc caaaccgatt ttaaaagaag taaatgacag actttcattc
     6781 ttggaaaatg tgggactaga ctatctaact ttgagtcgcg cttccggaac attatctggg
     6841 ggagaagcgc agcgaatcag attggctaca caaattggat cgaatctttc tggagttttg
     6901 tatattttag atgaaccatc gatcggattg catcaacgcg acaacgatcg actgattggc
     6961 tcattaaaga aaatgcgtga tcttggcaac accctcgttg tagtcgaaca tgatgaagat
     7021 acgatgtggg cagcagatta tttgattgac gtcggacctg gggccggaga acaaggcgga
     7081 caaatcgtgg ctgcaggcac tcctgaagaa gtagctaatg atgaaaattc tttgacaggg
     7141 aaatatcttt cagggaaaaa atcaatccct gtaccaaaag aacgccgtaa aggtaatggg
     7201 aaagcaatca agatcactgg tgcttcagaa aacaatctga aaaatattag tgttgaattt
     7261 ccattaggag aatttgtagc agtcacaggt gtgtctggtt ctggaaagag tacattggtc
     7321 aatagtatct taaagaaatc gttagcgcaa aaattaaata agaattctgc taagccaggt
     7381 aaattcaaga caatttccgg ctacgaaagt atcgaaaaga tcatcgatat cgatcaaagc
     7441 ccaatcggcc ggacgccgag aagtaatcca gcgacttata caagtgtatt tgatgatatc
     7501 cgtgggttat ttgctcaaac gaacgaggca aaaatgcggg gttataagaa agggcgtttt
     7561 agtttcaacg taaaaggcgg tcgttgtgaa gcttgtcgcg gggatggaat tattaagatc
     7621 gaaatgcact ttttgcctga tgtctatgtt ccttgtgaag tatgtcatgg taaacgatat
     7681 aattctgaaa cattagaagt gcattacaaa ggaaaaagca ttgctgatat tttggaaatg
     7741 acagtagagg atgctgtaga attcttcaag cacattccaa agattcatcg caaactgcaa
     7801 acgattgttg atgttggctt aggttatgtg acgatggggc aaccagcaac gacattgtcc
     7861 ggtggtgagg cacaacggat gaaacttgcc agtgaattgc acaaaatctc taatggaaag
     7921 aatttctata tactagatga accaacgaca ggacttcata gcgatgacat cgcccgcttg
     7981 ttgcatgtat tacaaagatt agtagatgct ggtaacacag ttttagtgat tgaacacaat
     8041 ctagatgtaa tcaaaacagc agattatatc attgatttag gaccagaagg tggagaaggt
     8101 ggaggaacga tccttgcgac tggaacacca gaagaaatca ttaacgtaaa agaaagttat
     8161 acaggtcact atttgaaaaa aataatggta taaagaggat agatagctga gatagttaaa
     8221 aaaacataaa aaaatgaatt tttgcttgtt ttattaatat cagtgttcta gaatcattac
     8281 atggtagttc tttctacact gattggtata gataaaaact tcctataagt tcaatccgtt
     8341 caaaaaagaa ttgagcaatc ggaggacttt cctttgattg cttttttctt tatggtatga
     8401 tattccttgt gtagttggaa aagaaaaaca agagattatg agtaagtgag agataatgat
     8461 gaacagtatt gaaaatgaag aatttatccc tatattatta ggaagcgaca tgaacgttta
     8521 tggtatggca cgttctttta atgaagcata cggcaaaatc tgccaagcat atgcttcgga
     8581 tcagttagcc cctacccgct acagtaaaat tgtcaatgta gaagttattc caggatttga
     8641 taaagaccct gtatttatcg agacgatgct tcgtttggca aaagaaagat attctgataa
     8701 gagtaaaaaa tatcttttga ttgcttgtgg tgatggatat gcagaattga tctcccaaca
     8761 taaacaagag ctgtcagaat attttatctg tccatatatt gattattctt tatttgaaag
     8821 gctgatcaat aaagtaagct tttatgaagt ttgtgaagaa tatgatcttc cgtatcctaa
     8881 aacattgatc gtcagagaag aaatgttagt aaatggacat ttggaacagg aattgccttt
     8941 tgaattccca gttgctttga aacctgcaaa cagtgtggag tatctttctg tccaatttga
     9001 aggaagaaaa aaagctttta ttttagagac tagagaagag ttcgatttaa ttttaggccg
     9061 gatatacgaa gccggttata aaagtgaaat gattgtacaa gattttatcc cgggtgatga
     9121 tagtaatatg cgtgtgctaa atgcgtatgt agatgaagat catcaagttc ggatgatgtg
     9181 ccttggtcat ccactattag aagatcctac accagcatcg attggtaatt acgttgtgat
     9241 catgccagat tataacgaaa aaatctatca aaccattaag gcatttttgg aaaaaatcga
     9301 atatacaggc tttgccaatt tcgatatgaa gtatgatcct agagatggag aatataaatt
     9361 atttgaaatc aatttgagac aaggtcgtag cagtttcttt gtgacattga acggcttgaa
     9421 tctggctcgt tttgtcacag aagatcgagt gttcaacaaa ccctttgttg aaacgaccta
     9481 tggaacgaat caatcagata aagcaagatt atggatgggt gtgcccaaaa aaatcttttt
     9541 agaatatgct cgggaaaatg aagacaaaaa actagctgag caaatgatca aagaaaatcg
     9601 ttacggtact actgtatttt atgaaaaaga ccgatcaatc aagcgctggt tgttgatgaa
     9661 atatatgttc cataactata ttccaagatt caaaaagtat tttcatgtga aagaaggcta
     9721 gttcatggag aattttttca gtattttagg cggaatgggc acgatggcga cagaaagttt
     9781 tgttcgcttg atcaaccacc gaacgaaagc tacaaaagat caagaatatt tgaattatgt
     9841 cttattcaat catgcaacag ttcctgatcg tacagcttat attttagatc gatcagaaga
     9901 aaatccaatg ccgtttctgc tggatgatat tgagaaacaa aatttattgc gaccaaactt
     9961 cattgtgttg acttgtaaca ccgcacacta cttttttgaa gagttacaag cggcaacgga
    10021 tattcctatc ttgcatatgc caagagaagc agcaaatgaa cttgtccgtc agcatacgac
    10081 agggagagta gcgattctag gaacagaagg aagtatgaaa gctggaatct atgaacgcga
    10141 agtcaaaaat cttggtttcg aaacagtgat tcctgatact gcgcttcaag aaaaaatcaa
    10201 ttacctgatc tatcatgaga tcaaggaatc ggattatttg aatcaagagc tctattacga
    10261 aatcttagag gaagcggtag agcgcctaaa ctgtgaaaaa gtcatcctag gttgcacaga
    10321 attatctttg atgcatgaat ttgctgaaga taatcattac ccagtgatcg atgctcaatc
    10381 tattttagcg gatcgaacga tcgaacgtgc attagctgaa agaagcgagg cattggatac
    10441 agcatcggaa aagtaaaaaa attggatttg gggcaaatat cctaaaaga
//
LOCUS       DARBDV010000095        10193 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000095 DARBDV010000000
VERSION     DARBDV010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.097"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            120..626
                     /locus_tag="SCM56_002395"
     CDS             120..626
                     /locus_tag="SCM56_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEG1111139.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            643..1542
                     /locus_tag="SCM56_002396"
     CDS             643..1542
                     /locus_tag="SCM56_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HEG1111140.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1629..2513
                     /locus_tag="SCM56_002397"
     CDS             1629..2513
                     /locus_tag="SCM56_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEG1111141.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2705..2776)
                     /locus_tag="SCM56_002398"
     tRNA            complement(2705..2776)
                     /locus_tag="SCM56_002398"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2741..2743),aa:Arg,seq:ccg)
     gene            2836..4170
                     /gene="rpoN"
                     /locus_tag="SCM56_002399"
     CDS             2836..4170
                     /gene="rpoN"
                     /locus_tag="SCM56_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEG1111142.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4319..5410
                     /locus_tag="SCM56_002400"
     CDS             4319..5410
                     /locus_tag="SCM56_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HEG1111143.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            5451..6452
                     /gene="gap"
                     /locus_tag="SCM56_002401"
     CDS             5451..6452
                     /gene="gap"
                     /locus_tag="SCM56_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEG1111144.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6562..7752
                     /locus_tag="SCM56_002402"
     CDS             6562..7752
                     /locus_tag="SCM56_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEG1111145.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7856..8611
                     /locus_tag="SCM56_002403"
     CDS             7856..8611
                     /locus_tag="SCM56_002403"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEG1111146.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8727..10025
                     /gene="eno"
                     /locus_tag="SCM56_002404"
     CDS             8727..10025
                     /gene="eno"
                     /locus_tag="SCM56_002404"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEG1111147.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3565 a         1886 c         2112 g         2630 t
ORIGIN      
        1 ttttgtcaca ccttcttttt tgtatataaa aatcccaagt aaatacataa aaaataaaaa
       61 aattgaaact tttagcgcac cttgtacgtc atattaagtg aggcagaaag gagtggagaa
      121 tgatttttac tggatataag aaaaaacggc agatcgaaaa ataccgtcac ctgctgaatg
      181 gactcatcga aactgattat caaaaaatgt tccgtatcac gatgaattac gtacatgata
      241 aagaagaggc cttagatgtg atgcaagata gttttcataa agccctgaac tattttgaaa
      301 aagaaaaaga gatcgagcat tttagtgcat ggttttatcg gatattgatc cgcacggcat
      361 tagatagttg gcgcagacaa aaaaggacgc ctgtggactt atttgaaatt caagatatcc
      421 cccaacagtt acctttagaa acttccgttg cagagctcca tatgatttta gataaaatag
      481 acagtccgga aaaagagatt ctgatcctct acttttttga aggatttcgt ttgaaagaaa
      541 ttgcgactat cttggatatg aatgaaaaca cagttaaaac gaaaatgtac cgctcattga
      601 atgctttacg tcaaatccta gaatgaagga gagaagagtg ccatgtattc ccaaaaagaa
      661 aatttaaaaa aactaaagaa aacttataaa gaacaagcaa ttcccaaagg gcttaaagag
      721 gagatccgcc agcgttttat tatcgaagag caatcattta taaaagcaaa gcggagaaag
      781 agacgtctcc aagggttcgg agtatctttg gctatcgtaa tgattacaac aagtagcctt
      841 ctttttaata gtcaagttcg aagctttgca gaagatttac ccatcttagg atcagtcatt
      901 gaattgatct taggcgaacg ctttactgat cgatctgaaa aaatcgatat tcaggtaccg
      961 aagatcaata cacaaaatga gaaagaaaat aaaacaattc atggattaaa tcaaaaatac
     1021 tttcgcgaag gacaagcaga ttttgaaaaa gctgaaaaag aatatggaaa ttttgaaacg
     1081 gatcattacc aagttttggg agattatcag aaaatcgtag acgataaccg gtttctcgtg
     1141 attgaaaggc agatcaccca aacagcagca gatagccatg ttgaaaaaag atacgacaca
     1201 atcgacaaga aaaacagtgt ccagctctca cttccacttt tattcaaaga tgacacctat
     1261 ctatctgttt tgacaaaaga agtcaaacgg cagatggccg aacaagtcaa ggaagatcca
     1321 tctaagtact attggactga gcaagatatt caagaaggga cgatagaaaa accaacactt
     1381 gttacgccta cacgaagttt ttatttgaac aaagagcatc aattagtttt gactttttct
     1441 caatacgaaa ttgctcctgg ttatatgggt acaccagaat ttgtcattcc taagtccgtg
     1501 acaaaaacga ttttggcatc ggaagattat ttggatcatt agacgagaag tagttgcgac
     1561 tagcagtttc aattttcaag taagtacaag taaataataa gaagagaaaa agagaaagaa
     1621 gagatatagt gaaaagaaat tatttagtta tcagtattat tgctttatcc gtgcttttgt
     1681 ttttcgaaat tacttcggtg gcctccttga tcaccgaagt aaaaggacaa acacactctt
     1741 cggagcattc gtcacaatct ttcaaacaat cagaacaatc aaccattaaa agccaatcaa
     1801 ctgaaacagc taacgtcaaa gaagccattc aagaatcagc ggaaaaggta caaagtcagt
     1861 catcacaatc aacacaagac agtacaaaaa cgagccgaga tccggctgtc aaaagcgtcg
     1921 caatcagctt tgatgatgga ccaggagcga ccacaacacc gcagcttctg cgaatcttaa
     1981 aagaaaaaaa cgtgcatgcc acttttttcg tcttaggaga aaatacggcg cagcatcctg
     2041 aaatcgtgaa gcagacagct gaagcagggc acgagattgg caatcacaca tacgatcatc
     2101 agaatctggc cactctttct gcccagtcga tgacggagga agtgacgaaa gcagacactg
     2161 agatcaaaaa agtcactggc aaaaccccca cttttgttcg tccgccctat ggttctgtca
     2221 cgaatgtagg agcaacgatc atccagcgac cgatcatcga atggtctgtt gattcagaag
     2281 attggaagac aagaaatcct gatttgattt tgcagaagat ccaagcaaca gtatacgatg
     2341 gggcaattat tttattccat gatatctatc ctgaaacgat tcgtgccgtt cctcaagtga
     2401 tcgattatct gaaagagcaa gggtatcgca tcacgacagt tggggattta ttagggcatc
     2461 caacggctgt cgagaactat tatggcagaa acgatcatcg gccagttcag taagtgagac
     2521 aagtatcttt aataaataac acattttaat aagtagttga acacataact accttacttg
     2581 caaaggctta acaatatcta tacaggtaca acaaaaaact gtccaccaat tttctttggc
     2641 ggacagtttt cacttaaaaa taaagaagca actattcgta aattatgcta aaatagcagt
     2701 agaacgcgtc ctgagggaat cgaacccccg tctcaagaac cggaatctta cgtgatatcc
     2761 actacactaa ggacgcaagc actgggataa ttgtaactaa acagataaaa aattacaagg
     2821 ggattggaca aaaaaatgaa atttgagcaa aatttttcac aaaaacagca gcagacacag
     2881 aaactagcca tgacgcaaca gttgcaacag tccatccaag ttttacaata taacagtgag
     2941 gagctgttgg cattcgttga gaatcaagca atggaaaatc cattggtgga agttgttgag
     3001 ccggaatggc agccagatta cataaaagct tcttcatctt cttatgaagg agaagagaca
     3061 aattacctaa atcagattcc tgatacaaaa gggtctttat ttgattcatt gatcgaacaa
     3121 gtccatttga attaccgaga tacttttttg aggaaaatcg ttctttatct tgtagaatac
     3181 atcgatttga acggattttt gacgattagt ttagaagaag ccggaaaaga gattggagct
     3241 acgcctatcc aaatgctcga tgcgttgact ctgattcagc aattagaacc agctggagta
     3301 ggtgcacgta gtttgcaaga atgtctcatg cttcaaacag aacgagatga ttatgcgccg
     3361 gaattagctt atatcgtgtt ggaagaatgt tttgaggaac tggtggagcg aaaatggaaa
     3421 gaaatcgcac aacgtttttc tgttgatcta catgctgttc agcagatatt cgactattta
     3481 caaacgttga gtccaagccc tggccggatt ttcgaccgta gcagcgaatt gtacattatt
     3541 cctgatgtcc gggtattagt ggacgacgat aaaaatgtac aagttatctc aaaccgaaaa
     3601 aatcagccaa atatacgctt tcaagagagt tacttcaaac aaatgtcaca acaagcagat
     3661 aaagaaactg aaaattattt aaaagaacgg aaacaggaat tcgaatggtt gaagaaaacg
     3721 atcctacaac gtggggatac aattttgcgg gttgctcaag tcattgtttc ccggcaaaaa
     3781 gctttcttta tcgacaagga acgtccaatc aagcctctta cgttaaagga agttgcaact
     3841 gagatagatg tgcatgaatc cacggtcagt cgtgcagtca acgggaaata tttggaaaca
     3901 gacttcggtg tatttgaatt aaaaaaattt ttcactacac gaattcctac gaatagtaca
     3961 gaacagacag aagacctttc agcagataca gcgaagaaaa agctgcagga attagtcgat
     4021 caagaagaca aaaacaagcc gttgtctgac cagaaattgg ttgaattgct gaaaaaagat
     4081 gagatagcga tctcaagacg aacagtagca aaatatcgtg atttattagg aatcccgagt
     4141 tcatcaaaaa gaaaacgtta tgataactaa tcagtcaaaa gactagaaaa agtatttcac
     4201 tttttctagt ctttttagtt gaaaaacgag agcaaaactg ttagacttac agtcgtgagg
     4261 ttgagatttc cctttattga tgtgaaattc gaatagttaa tttctttttt tatcgtatat
     4321 gggtcgtttt aagtctacat tggacgtaaa gtgtccaact aaggagatcg ctatgctgga
     4381 tgaaataaaa atgattgaat ctgttgcacc agatattatc gaagtgttgc aagaacgcta
     4441 taaaatttta cgaaatatct attggatgca gccgattgga cgtcgaagtt tatcggaaag
     4501 tatggggatc acagaacgag tattgcgaac agaaacagat cttttgaaaa atctgaattt
     4561 gatcgacacc tcaaaaagcg gtatgacgct gacaactaag ggcgaagaag tttatcaaag
     4621 ccttgaaaac ttcatggatc agcttttagg tacgcatcaa accgaacaac agttggcaga
     4681 atattttggt aaccaacgct gtatcgttgt ttcaggaaac agcgaagagc agaccaaagt
     4741 ggctgatgca ttcggcgaag cgctgagcga agccctagac cgactacttc cagaaggcga
     4801 gaatatcatc gctgtgatgg gaggaactac tatggctgtg gtagccgaac aattgtctaa
     4861 tctggaaaat aagaaacggc ataacctgtt tgtccctgca agaggcggga ttggtgaagc
     4921 gataactgta caggcaaatt ctgtcagtgc aagaatggct gctaaagcaa acggtaacca
     4981 ccgtgcactt tatgttccgg aacaattaag tctggcaact tacaattctt tactcaatga
     5041 gccatctatc caagaagtct taaacttgat tagtgaagcg aattgtgtta ttcatagtat
     5101 tgggcgtgca ttacacatgg cagcacgtcg taagatgact gaaaaagaat tagtcatgct
     5161 gaaacaagca aatgctgttg cggaatcttt tggctacttt ttcaatgaaa aaggtgaagt
     5221 cgtttataag gtccctagaa tcggcataga attgcaagat ttggaaaaag tcccgatcat
     5281 catggcaatc gcgggcggaa aatcaaaagc caaagcaatt cgagcttata tgaaaaatgc
     5341 accaaaacaa acgtggctca tcactgatga agctgctgca aatgagattt taaaaggggt
     5401 aaccctttaa aataaatatt tttttgattt tcataaggag gaaatcttaa atgacagtta
     5461 aagtaggtat taatggtttt ggacgtattg gacgtctagc attccgtcgt atccaagatg
     5521 tagatggaat cgaagtagta gcaatcaacg acttgacaga tgctaaaatg ttggcacact
     5581 tgttaaaata cgacacaact caaggacgtt tcaacggaac agttgaagtt catgaaggtt
     5641 cattcaacgt aaacggaaaa gaagttaaag tattagctaa ccgcaaccca gaagaattac
     5701 catggggcga actaggcgta gatatcgttc tagaatgtac tggtttcttc acttctaaat
     5761 cagcagctga aaaacattta acagctggtg ctaaacgcgt tgttatctct gctcctggcg
     5821 gaaacgatgt tccaacaatc gtttataaca caaaccacga aacattaact ggtaaagaaa
     5881 cagttatctc aggtgcttca tgtactacta actgtttagc tcctatggct aaaacattga
     5941 acgacaaatt tggtgttgtt gaaggattaa tgacaactat ccacgcttac acaggtgacc
     6001 aaatgactct agacggacct catcctaaag gtgacttccg ccgtgcacgc gctgctgctg
     6061 caaacatcgt tcctaactca acaggtgctg ctaaagctat cggtttagta attcctgaat
     6121 tgaacggtaa attagacgga gctgctcaac gtgttcctgt accaacaggt tcattaacag
     6181 aattagtaac agttcttgaa aaagaagtaa ctgttgacga aatcaatgca gcaatgaaag
     6241 aagcttcaaa cgaatcttac ggatacaaca cagacgaaat cgtttcttca gatatcgttg
     6301 gtatgactta cggttcatta tttgatgcta cacaaactaa agtaatgaca gttggcgaca
     6361 aacaattagt taaaactgtt gcttggtacg acaacgaaat gtcatacact gcacaattag
     6421 tacgtacttt agaatacttc gctaacttat aagatagaca aacgtacaag tgtaaattat
     6481 agacatgtac atttaaaagc ggggaagcaa tcgcgcttct tccgcttttt ttgataataa
     6541 gtttattcag gaggaaataa aatggctaag aaaacagtga aagatatcga tttaaaagac
     6601 aaaaaagttc ttgtccgtgt tgacttcaac gttcctttga aagacggcgt gatcactgac
     6661 gacactcgta tcaaagcagc attgccaaca atcaactacg ttctagaaca aggcggaaaa
     6721 gcaatccttt tctctcacct tggacgtgta aaaacagaag aagataaaga aggaaaatct
     6781 ttagctccag ttgcaaaacg tctaggcgaa ttattaggta aagaagtaac attcgtacct
     6841 gaaactcgcg gagaacaatt agaagaagca atccgcaaca tgaatgacgg cgacgttgtc
     6901 gtattcgaaa atacacgatt tgaagatatt gacggtaaaa aagaaagcaa aaatgataca
     6961 gaactaggta aatactgggc ttcattaggc gatgtatttg ttaacgatgc tttcggtaca
     7021 gctcaccgtg cacatgcttc taacgtaggt atcgcttcaa ctggtatccc aacagttgct
     7081 ggattcttga tggaaaaaga aatcaagttc atcggtgaag cagtagaaga accaaaacga
     7141 ccaatgattg caatccttgg tggcgcaaaa gtttctgata aaatcggcgt aatcgaaaac
     7201 ttgattccta aagcagataa aatcttgatc ggtggcggaa tgacttacac attctacgaa
     7261 gcaaaaggaa tcaagatcgg taactctcta gtagaagctg ataaagtgga actagcaaaa
     7321 gaattaatcg aaaaagcagg cgacaaactt gtcttgccaa tcgacaatgt ctgtgcacca
     7381 gaattttcaa atgatgttga aacacaagta atcgaaggag acattcctga tggtttgatg
     7441 gctttagaca tcggcccagc atcagtaaaa ttatttgctg atacattaca aggtgcaaaa
     7501 acagttgttt ggaacggacc tatgggtgta tttgaaatga gtaactttgc taaaggtaca
     7561 atcggtgttt gtgaagcaat cgctaactta gaaggcgcaa caacaatcat cggtggcgga
     7621 gactcagccg cagcagcaga acaattagga tttgctgaca aattcacaca catctcaaca
     7681 ggtggcggcg caagcttgga attgcttgaa ggtaaagaat taccaggact agcagcaatc
     7741 aacgataaat aaacgaccaa gtttgctcaa ttctgacgag taatcgatca aaaggtgaag
     7801 ggagttttca tgactcccta gccatttttc aataaattaa ggagtgtgtt cctcaatgcg
     7861 taaacctatt atcgcaggta actggaaaat gaacaaaact gcatcagaag caaaagaatt
     7921 tgctgaagca gtaaaaaaca aaatcccttc aaatgacaaa gttgattcag tcatcggatc
     7981 tcctgcatta ttcttacaag aattaatgac atcagctgaa ggtacagaac taaaaatctc
     8041 agcacaaaac tgctactggg aaaactcagg tgcgttcact ggtgaaactt caccagcagc
     8101 tcttgctgat ctaggtgttc attatgtgat catcggacat tcagaacgtc gtgaatattt
     8161 ccatgaaaca gacgaagaca tcaataaaaa agcgaaagct atctttgcaa atggtatgac
     8221 accaatccta tgctgtggcg aatctttaga aacctatgaa gcaggcaaaa ctgctgaatg
     8281 gatcgaagga caaattacag ctggtttagc tgatctttca gctgaacaag tgtcaaacat
     8341 ggttattgct tatgaaccaa tctgggcaat cggtactggt aaatcagctg acgcacaaat
     8401 cgctgacgaa atttgtggag ttgtacgtca aacagttgaa aaactttatg gaaaagaagt
     8461 atcagaagca gttcgtatcc aatacggcgg ttctgtaaaa ccagaaaata ttgctgaata
     8521 catggcaaaa gaaaatgttg acggtgcttt agtcggtggc gcaagcttgc aagcagattc
     8581 attcttgtca ttgttagacg ctgtaaaata attgttgaaa atttgcgaaa aatgcaagaa
     8641 atgtttgcac taatgagcaa aattaactaa aatcgtatat gagggaaatc ccttaggaaa
     8701 aaacaaactc aaaggagaga caaaacatgt caattattac tgatgtttac gctcgcgaga
     8761 tcttagactc acgtggtaac ccaacaatcg aagtagaagt atacacagaa agcggagctt
     8821 ttggccgcgg aatggttcct tcaggtgctt caactggtga atacgaagca gttgaattac
     8881 gtgacggaga caaagctcgt tatggcggta aaggtgtaac taaagctgtc gacaacgtaa
     8941 acaacatcat tgctgaagca atcattggct acgatgttcg tgaccaaatg gctatcgaca
     9001 aagctatgat cgctttagac ggaactccta acaaaggtaa attaggtgcg aacgctattt
     9061 taggtgtttc tatcgctgtt gcacgtgcag ctgctgacta cctagaagta cctttatacc
     9121 actacctagg cggattcaac acaaaagtat tgccaacacc aatgatgaac atcatcaacg
     9181 gcggatcaca tgctgataac tcaatcgact tccaagaatt catgatcatg cctgttggcg
     9241 ctccaacatt caaagaagct ctacgtatgg gtgctgaagt attccacgca ttagcttcaa
     9301 tcttgaaagc tcgcggatta gcaacttctg ttggtgacga aggtggattt gctccaaacc
     9361 ttggttcaaa cgaagaaggt tttgaagtaa tcatcgaagc aatcgaaaaa gctggctatg
     9421 tacctggtaa agacgttgtt cttgctatgg atgctgcttc ttcagaattc tacgacaaag
     9481 aaaaaggtgt ttacgtacta gctgattcag gcgaaggcga aaaaacaact gacgaaatga
     9541 tcaaattcta cgaagaatta gtttctaaat atccaatcat ctcaatcgaa gacggattag
     9601 acgaaaacga ctgggacgga ttcaagaaat taactgacgt attaggcgac aaagttcaat
     9661 tagttggtga cgacttgttc gtaacaaaca ctcaaaaatt atctgaaggt atcgaaaaag
     9721 gaattgctaa ctcaatcctt atcaaagtga accaaatcgg tacattaaca gaaactttcg
     9781 aagctatcga aatggctaaa gaagctggct acacagcagt tgtatctcac cgttctggtg
     9841 aaacagaaga ttcaacaatc tctgatatcg ctgttgcaac aaacgctggc caaatcaaaa
     9901 ctggttctct atcacgtact gaccgtattg ctaaatacaa ccaattacta cgtatcgaag
     9961 accaacttgg tgaagttgca gaatacaaag gtttgaaatc tttctacaac ttaaaaaaca
    10021 aataattctt ttctaatgaa gatacatgat cgactattga aagatagtcg agtatgatca
    10081 cttagttaag taatcgaact ctctacttgc tgaaatatcg gctctttgtc aataaggact
    10141 gatgagttgt gcaaaatcaa atctgagtca gaatgaacca cattctggct cag
//
LOCUS       DARBDV010000096        10108 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000096 DARBDV010000000
VERSION     DARBDV010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.098"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(572..796)
                     /locus_tag="SCM56_002405"
     CDS             complement(572..796)
                     /locus_tag="SCM56_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111148.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            987..3764
                     /locus_tag="SCM56_002406"
     CDS             987..3764
                     /locus_tag="SCM56_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG1111149.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3845..4264
                     /locus_tag="SCM56_002407"
     CDS             3845..4264
                     /locus_tag="SCM56_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HEG1111150.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4276..4746
                     /locus_tag="SCM56_002408"
     CDS             4276..4746
                     /locus_tag="SCM56_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1111151.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4763..5542
                     /locus_tag="SCM56_002409"
     CDS             4763..5542
                     /locus_tag="SCM56_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1111152.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5529..6350
                     /locus_tag="SCM56_002410"
     CDS             5529..6350
                     /locus_tag="SCM56_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG1111153.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6367..7416
                     /locus_tag="SCM56_002411"
     CDS             6367..7416
                     /locus_tag="SCM56_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEG1111154.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7429..8433
                     /locus_tag="SCM56_002412"
     CDS             7429..8433
                     /locus_tag="SCM56_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEG1111155.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            9066..10085
                     /locus_tag="SCM56_002413"
     CDS             9066..10085
                     /locus_tag="SCM56_002413"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEG1111156.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3393 a         1660 c         2100 g         2955 t
ORIGIN      
        1 ttttacttaa tttttctata aatgaattct gcataaaaaa tatatcaagt aaatgtttat
       61 agatgaaact ctatttactt cttttttaat ttgttcttgc aatctttttt gctacgtaat
      121 ttttgtaaaa aagtgggttg agagaaatct aattgatttt caaagaagga gtatgaaaat
      181 gagaaaaata tttattcgtt tattgtgggt actatcttag aggaataaat aatagtggag
      241 aaaactatgg tttggcgcag attctatcag gtttgatgga acgaagacaa cctcccgaca
      301 tcaagtcaaa agctacttga gattttgatc tgaaaataaa aaaagaaaac aaggtaggaa
      361 catcaatgtt tctaccttgt tttcgtaatc aatgggaaaa aactaagagt ggtgtacgta
      421 atcaataaaa aggatggtga caacagtacc tctttactct ctgaaagcat cataactcgt
      481 ttttcctcat tatgccaata aattgttgtt ataccacata taaggatttg taatagctaa
      541 aaacagacag gaaagttgaa atctgtgaat cttattttgt atggaaggat ttatagaacg
      601 caaaaatcaa gataaagatg aataaaaatc ctgtttcggc tatatattgc gtatctacgc
      661 caataaaacg agtttgttct gtcatagcca tataaagcaa aggcaaaaat acaactacag
      721 cgagataaga tccgatgatt tctcgaaaag ttttccagga agtatgtttc ctgtttttat
      781 atttatccga tttcattgaa tagtcatagc ctccttattc tttcatatgt tgaaattcca
      841 gtataaaaac tggaaggaac tgtacgtttt ttagaataaa aattataagg gataagtagg
      901 ctgctgtcta taataagata tatttttcaa ttctttatta aagattttat tgacaaaatg
      961 aatccgtttt ctatgatgga ggaggggtga ataagttgtt gaagaaggaa attttatgtt
     1021 acttagagaa ccagacagcc tttttcgatt tagaacatat gaacgaggtc tttacagcac
     1081 aaaggttagc tgatcatttt ggggtaaaaa gaaacacagt cagtcattat ttgaaccagt
     1141 taaatgatga gcaactacta gtcaaaatca attctcgacc agtgatttat ttccacaaag
     1201 aagcttttga aaaacaattt tttaagttac gcacgaattt ttatttttct atcgatgatc
     1261 tcaaagagga acagcctttt ttcgcgcaac caaaagactt attctctttt atgatcggat
     1321 acgacgccag tttgaaagaa agtatcgaac aaatcaaaac agctctttac tatccagatg
     1381 gcggattacc acttttgatt acaggcgaaa gtggcacagg taaaagttat ttagttaatt
     1441 tggtctatca gtattgtctg cttcatgatc tgctagaaga ttctgcacca ttcatcactt
     1501 ttaattgtgc acaatatgca gataatcctg aacttttgac tagcaatttg tttggacatg
     1561 ttaagggagc gtacactggt gcagaagaaa ataaaaaagg ggcttttgaa gcagcagatg
     1621 gtgggatctt gtttttagac gaggtacatc gcttaacacc agaaggtcag gaaaaactgt
     1681 tcacctatct tgatcaaggt gtgatttatc gtatgggaga acctaatttg tcacgccgga
     1741 tcaaaacacg tctctttttt gctacgactg aagagattac tagtcatttc ttaacaactt
     1801 tcattcgaag aatacctatc cgaatcgaac tacctgcact taatcagcgt agtcgtaatg
     1861 aacggctaga actgatttac tctttttttc tcgaagaaca aagaaaaatg gggagccctt
     1921 tgtcagtcag tggaccagct ttgtcacttt taacaagtca gaaattatcc ggaaacatag
     1981 gagagctgaa aaatatcgtc aaagtttcag cagcaaaagc atatgctgaa cagcgtgaac
     2041 agcctgaaat ccatgtcacg atccatcatt tgcaaaaaga actacttgcg cagcctatta
     2101 ctaaaaatac agcacagcaa ggaatctata ttacgcaaga ccagacctta gagcaattaa
     2161 tggagaagca acagcccgaa cagcagcgaa tcgttaaaag ttttgagcaa atcctaattg
     2221 atttcaagaa aaataactgt ctgctatatt cttgcgaagg aaaattaaag caagaagtga
     2281 ttcaattatt tgattttttg ctgtttgaga ccagtagaca agaaaaacac gagctgctgg
     2341 tttattggac acaatctatc cgagagacct ttcgacaaat ggaaactgcc tatcaaatca
     2401 aattcgatgg aaacagtgtc tatgccttga gttactatct ctatcagcga agcactatca
     2461 aatggttacc ggaagaacat gatattatcc aactaatcaa agaattagaa caacaagttt
     2521 ctcagtctta tccatctagt tatcactatg tccaacgaat tctggaatta agtaagatca
     2581 aattagatat tgaagacaca agtatggatc ggattttact gacgatctat ctaaaaaaag
     2641 cgaaatggtc aaaagaaaaa cgactgccaa aagcgatgat tgctgctcac ggttatgcaa
     2701 ctgctagcag tatggcaaat gtagtaaatc gtttattaca agaggatcta tttgaatcat
     2761 ttgatatgcc actagatgtc acaccacagc agattgcttc agaaatcctt gattattgcg
     2821 aaaacagcga tgtttcaaat ggattgatca tattagtaga catgggatct ctgaaagaaa
     2881 tccatcagtt ttttaaaaaa cagttatccg taccgctttt gattttgaat aatgtgacga
     2941 cacctttagc aatcacagta ggggaatgcc tgcaaaaaaa tgtatcatta gaagagatag
     3001 cagaagagac tgttcgacag atccagccag aatggcgact gctctatcca gaggaaaaca
     3061 aacccaaagc actcattact acttgcttca ctggaatcgg tacggcggtc cacttaagtg
     3121 aactgctaga aaaaagctta cctacggctt gtcagttgaa aatcatccca tatgaatatc
     3181 agcaattaaa agataagaaa aacagtgacc cattattttc catatacgaa gtcgtaggaa
     3241 tgattggcac cacagatcct gatatcacca atatcgacta tctttcatta gaagacctca
     3301 tctcggggga aaagatgtct gtactagctg aatggttgga cactactatg aatgcttcag
     3361 agaaggaaat attcaatcag cgcatcattc gtaatttttc attggaaaaa gtactggatt
     3421 ccgtgactat tctagataca gagaaggtga tgggagaaat cgatcatttt atgagagaat
     3481 tagaggtgca gttgaatcga agcatcagca atcccaaaaa attagcgttg tatgtccatg
     3541 tgagctgtct cattgaacga ttgatccgac aaatgccaat cgaaacctat caaggattag
     3601 agaaatggaa gcagtgtcaa aaagaagggt tatctgctat taaatctgcg tttagtgtca
     3661 ttgaagagca ttatagtgtc atgatcccca attctgagat tgcctatatc tatgatattt
     3721 tgagtgaaga tacagagttt ctctcgatag aagaagaatt ttgaacataa gcaagttgtt
     3781 gccactaatt ttggcatgca acttgctttt ttagtatgtg taagtgaaaa gaggagatga
     3841 aaaagtggat agagaaatta tcttagcttc ccatggaaaa tttgcttcag ggatattaga
     3901 ttcattagag ctgatctatg ggaaaaatca ttctattact gtattcgatt gctatactga
     3961 tgagaatttt gatttagcgg aaacagtaag gcagcttttt atgaggtaca aagataaaga
     4021 aatcattgtt ctgacggatc tttttggcgg aagtgtcaac aacgaattct tacaatatat
     4081 caatcgggat catgtctatc tcgttgccgg attgaatttg ccactattga ttgagctagt
     4141 atctcggttg gacgctgaag tagagacaac tgttttgatc cagcaggtgc taactgattc
     4201 aaaaaaaatg atccagtttt gtaacgagcg gctcaatcag aaaatagaag aagacgaatt
     4261 ttaggaggaa gaaaaatgat tgtattgaca agagtcgacc atcggttatt acacggacaa
     4321 gtagcttttt catggacaca aacaataggc gcagactgta ttttgattgc taatgatgat
     4381 gtacctacga atgagatacg aaagacaacg atcaaattag ccaagccaca aggagtcaaa
     4441 ctagtgatca aatcaattga tgactcgatt gccgcgttga aaagcggggt gacagataaa
     4501 tataagttat tcatcgtagt ggaatcgatc gaggatgcat ataagctggc agaagcgtat
     4561 ccagaaatca aaaccatcaa cttaggaggg atcaaggcaa aacctggtac tcgcagcatc
     4621 ggaaaagcgg taaatatttt accagaagag gaggaacgct tgaaacgatt aattgacaaa
     4681 caaatagaag tagaaatccg tcaagtacca gcagataaaa aagtggatgt aagagaagtt
     4741 ttgtaagaaa ggaagaacct caatgattgt acaagcagtc ttattaggaa ttgttgcatt
     4801 tgtcgcacaa tcagaatatg cactagggac atcgttgctt tctcgtccga ttgtgacggg
     4861 gctgtttaca ggaatcgtgt taggtgatgt aaaaactggg atcattatgg gcgcaacttt
     4921 agagttagca tttatcggat cgttctcagt aggtgcttcg attccacctg atgttgtgac
     4981 cggtgggatt ttagggacgg cttttgctat tacagctgga gccggtacag aaaccgcgct
     5041 tttactaggt ttgccgattg ctacgttgac attgatattg aaaaatattt acttagggct
     5101 tttgattcca attatgaacc ataaagcaga caattatgca gaagaaggaa attataaagg
     5161 agtcgagcat atgcacttgc ttgccggtat cggtctttcc ttgatgcttg gtgttgtggt
     5221 aatggtttct tatatggtgg gaagcaatac gatcggaaat ctattaaata tgattcctga
     5281 cttcgttcaa aaggggttgt cagtagctac agggttgatt ccagctcttg gttttgcgat
     5341 gcttgctcgt ttgctgatca acaaacaagt tgcaccatat tttttcttag gttttgctat
     5401 agcttcttac ttagagattc ctgtgaccgg agtagctatt tttggtgcga ttttagcagt
     5461 tgtggtagtc aatattatga atgtccgtca actaccactt caaacaactg caggggaggt
     5521 agaagaagat gaagaattct gagatcaaaa acagcaaaat aacgaaaaaa gagctaaatc
     5581 acgtattttg gcgttctttt caaatggagt tttcttggaa ttatgaacgt cagatgaata
     5641 tggcttatgt ttatgcaatg attccgattt tgaaaaagtt atatcagtcg aaagaagaaa
     5701 tggcagcagc tttgaaacgg catctagagt tttttaacac gaccccacat atcgttacac
     5761 tgattttagg aatcacggct gcaatggagg aagaaaataa ggatgatcct gaatttgatg
     5821 tgactgcgat cgatagtatc aaaacatctt tgatgggacc tcttgcaggg ttaggagatt
     5881 ctttcttttg gggaactttg cgattgattg ccacaggtgt gggtacctct ttagccatgc
     5941 aaggaaatat tttaggccct atcttgttca ttttgatttt caatattccg cacattctgt
     6001 ttcgctatct agcaacaggt tggggatatc gcttaggaac gggttttttg aaaaagatcc
     6061 aggcaaacgg tatgatggaa agtttgacat taggcgcgtc tattattgga ctgatggtcg
     6121 ttggagcaat gactgccacg atgatcacca tcaatattcc tttaaaaatc ggttcaggag
     6181 aaaatgctgt gactgttcaa agtatttttg atgatatcgt tccaaatatc ttatgtcttg
     6241 gagcatttgg catagtgttt tatctattga agaaagaagt gaaaccatta acgatcttga
     6301 tcggactagc tttctttgga atttttggtt cgtggatagg gattttttaa aaaaggagca
     6361 gagaaaatgt ttacgatgca agattatatc tatgaagaaa aggaagtact aagtacgatc
     6421 ttgaaaaaga atgatttttc gacaagagaa catatgaaaa aaacagtgaa tttactgatc
     6481 ttagcaacgg gttcatctta taacgcttgt ttagcagcga agccagcatt ggaatcttat
     6541 ggtgatctta ctgtcgatat tcaagaacct ttttattttt tcaattatgg aaaactttct
     6601 ccttcaatcg atactgtgat tgctgtgtca caaagcggga aaagtgcttc tactgttgaa
     6661 gcagtaaaaa tgatacaaaa acaagggtta cctgttgttg ctatcacaaa tgacgtgcaa
     6721 agtccacttg ctttagaagc tgcacaaatt attgatttag gtgccggggt agaatcagta
     6781 ggcttcgtta caaaaggcta ttctgcgact gttttacagt tactactctt agggatagga
     6841 attggaataa gtaaaaataa aatcagcaaa aaaatagaac aagactacat gcaacagttg
     6901 cgcaaaataa tcaaccattt accagcgatt attcaaaaaa cggaagaatt ttttgatgga
     6961 tatcaaagtt tatttcgttt agcacagcgt ttcgtcacca ttggatatgg tccaaactgg
     7021 ggaacagcta aagaagcaga gacgaaattg actgaaacaa tccgcgttcc gtcacaagga
     7081 tttgagttag aagcttatat gcacggccca tacctggagg cagatgcttc gcatttgcta
     7141 ttctttattg aaggggaaag tgtaaataag gaacgttcgc aaaagttgca aagatatatg
     7201 agtcggtacg ttggagagac tctcaccatt accacaaaaa aagcaagaaa cgagaaaaca
     7261 cttggattag caatagaatg tgatgaatat ctgtctgtat tagcgcttgt cgttccgttt
     7321 caactattcg cttataaaac cgcagtagct aaaggtattg acttgaacaa aaagattttt
     7381 gaagactttg atacagtctt gaaaagtaaa ttataagagg tggaaatcat gctgaaattc
     7441 aatgaagaag aacaaatcaa agcaataaaa ggcgcactag ctttacgtcc gcaagtagaa
     7501 gaaatcatcg ataaacttta tgcggaaaaa tttgacgctg tctattatct aggaatcggt
     7561 ggaacgtatg cttcctcgat gcaagctgtc acttatatga atgggaaaag caacttgcct
     7621 gtctttgtac agcatgctgc agaatattac actactggaa acaaaagatt aacaaaagat
     7681 tctatcgtta ttttgtcctc tgttaccggt acaactcaag aagtagtgaa agcagtagaa
     7741 caaatcaaga aagtaggagc gacactgatt ggatttatcg ataaagcgaa cagtaagttg
     7801 tctcagctat gcgattttgt ggtcacttat ccagctcctg gaacagaaca aatcaaattt
     7861 tttatggcag cagatcgatt gatgtatttg aatggagaat tcgaagctta ttcagaatac
     7921 tatgaacagc tcgaacgtta tttaccaaca gggttagtgg aagcagagaa aaaagcagat
     7981 gcatttggct tatcatttgc agaaaaacat cgacgtgata gtatgcatta ttttattgga
     8041 gcagggaatc aatggggagc tgtttattca tatgccatgt gttactggga agaacaaagc
     8101 tggcttccat cgaaatctat ccatgctgcc gagtttcttc acgggacgtt agagatcgta
     8161 gaagaaacaa ccccagtcac attattttta ggcgaagatg aacagagagt gttatctgaa
     8221 cgtgtggcta aacttttgcc aaaaatttgc agcaattata cattgataga cacaaaagac
     8281 tatccagtag aaggtatcag tgaaaaatat cgcggacgtg ttttgtcttt cttattgatg
     8341 catgtagtga cacaacgcat cgatgctcat gtggagcagt tgaactgcca tccattagag
     8401 atacgccgct actatcgtca gtttgattat taggatagac tggataaaaa gaatataaaa
     8461 aataaagaga gcagcgataa ctgctccctt tattttttta aaatattggc atcaattacg
     8521 ttataaaaca cattttttgc attatctaca gttcaactta taatctgttt aatgggacat
     8581 gtgggtgcca accatttttt gttatgcaat agtactattt agttgttgca tggatccgta
     8641 gtaaaagtcc aagtacaacg agttgcgcca gtttacatag cgttttgtat ccagaatgtt
     8701 gactaaaaca aaagttccta tgtcaaccgt caacagagac aatctgtccg ttgcaggacc
     8761 tgacagattt agaaatgttc aatctgtctc ttatgtattt taaatcaata ctcactagga
     8821 gaagtttcct aacctcctaa tagtaatgct ggtagtaaca ttgtcaaaac tgcttcctct
     8881 taggaacaag caaagcataa cgagtgtaaa aataaaatgt agactattgt aacatatcat
     8941 tgattatttc aagtaaaaac gataaaagaa gtaaacaaac acttaaaaac aatggaaatt
     9001 agtcccaact ttaagaaatg tatactataa tggaaattat caattattta tttggaggaa
     9061 tatccatgaa acaaaaaatc gcagttcttg gtcctggatc ttggggcact gctttagcac
     9121 aaacattggc agaaaacggg catgatgtcc gtatttgggg aaatgtacct gaacagattg
     9181 atgaaatcaa taagtatcat acaaatcagc attttttacc tgatttgacg atcccagaat
     9241 cgattatcgg atataaagaa ttggcagaag cagtggataa cgcagatgct attttatttg
     9301 ttgttccaac aaaagcgatt cgttcagtcg cacaggaact tattggtaaa atgaatacta
     9361 agccaatcat tatacatgca agtaaaggac tagaacaaga tacgcataaa cgaatctcag
     9421 aagttttggc tgaagaaatt ccagaagaaa aaagacaggc aattgttgta ttatctggtc
     9481 ctagccatgc tgaggaagtg gcagtacatg atatcacaac aattacagct gcaagtattg
     9541 atcaaaaagc agcagcctat gtacaaaaac tgttcatgaa tgattatttc aggatttata
     9601 caaataatga tgtgattggc gtagaaactg gtgcagcact gaaaaatatc attgcaattg
     9661 gtgccggcgc tatccatgga ttaggattcg gagatgatgc aaaagcagca atcatgactc
     9721 gaggattggc agaaatcagc cgtcttggtg tcgcgatggg tgccaatcct ctgacattta
     9781 tcggtttgag tggtgtgggg gacttgatcg ttacttgtac aagtgttcat tctcgcaatt
     9841 ggcgtgccgg taacttgtta ggacagggac ataagctgga agaagttcta gaaaacatgg
     9901 gaatggtcgt tgagggtgtg gcaacgacaa aagcagcagt agagctagct caacagctag
     9961 gtgttgagat gccaattact caaacaatct ataatgtttt gtataatgga gaagatatca
    10021 aaaaagcagc aaaagaaatc atgttacgtg acggaaaaat ggagaatgaa ttttctcttc
    10081 gttaaataga tgatcaatag ttaatcgt
//
LOCUS       DARBDV010000097        10083 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000097 DARBDV010000000
VERSION     DARBDV010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.099"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            161..391
                     /locus_tag="SCM56_002414"
     CDS             161..391
                     /locus_tag="SCM56_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEG1111157.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            488..1789
                     /gene="murA"
                     /locus_tag="SCM56_002415"
     CDS             488..1789
                     /gene="murA"
                     /locus_tag="SCM56_002415"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEG1111158.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1802..1978
                     /locus_tag="SCM56_002416"
     CDS             1802..1978
                     /locus_tag="SCM56_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEG1111159.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2113..2409
                     /gene="yidD"
                     /locus_tag="SCM56_002417"
     CDS             2113..2409
                     /gene="yidD"
                     /locus_tag="SCM56_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEG1111160.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2473..2940
                     /locus_tag="SCM56_002418"
     CDS             2473..2940
                     /locus_tag="SCM56_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1111161.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3025..3909
                     /locus_tag="SCM56_002419"
     CDS             3025..3909
                     /locus_tag="SCM56_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG1111162.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4023..4259
                     /locus_tag="SCM56_002420"
     CDS             4023..4259
                     /locus_tag="SCM56_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEG1111163.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4347..4427
                     /locus_tag="SCM56_002421"
     tRNA            4347..4427
                     /locus_tag="SCM56_002421"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4381..4383,aa:Tyr,seq:gta)
     gene            4434..4505
                     /locus_tag="SCM56_002422"
     tRNA            4434..4505
                     /locus_tag="SCM56_002422"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4466..4468,aa:Gln,seq:ttg)
     gene            4630..4800
                     /locus_tag="SCM56_002423"
     CDS             4630..4800
                     /locus_tag="SCM56_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111164.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            5020..5727
                     /locus_tag="SCM56_002424"
     CDS             5020..5727
                     /locus_tag="SCM56_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1111165.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5787..6431)
                     /locus_tag="SCM56_002425"
     CDS             complement(5787..6431)
                     /locus_tag="SCM56_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEG1111166.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6558..6845
                     /locus_tag="SCM56_002426"
     CDS             6558..6845
                     /locus_tag="SCM56_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111167.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6953..7273
                     /locus_tag="SCM56_002427"
     CDS             6953..7273
                     /locus_tag="SCM56_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1111168.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7354..8667
                     /locus_tag="SCM56_002428"
     CDS             7354..8667
                     /locus_tag="SCM56_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEG1111169.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8664..9383
                     /locus_tag="SCM56_002429"
     CDS             8664..9383
                     /locus_tag="SCM56_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEG1111170.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9511..10062
                     /gene="raiA"
                     /locus_tag="SCM56_002430"
     CDS             9511..10062
                     /gene="raiA"
                     /locus_tag="SCM56_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEG1111171.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
BASE COUNT         3359 a         1668 c         2151 g         2905 t
ORIGIN      
        1 tttgtctcag tccctttttt tgcatatttt tagcatttat ttcctaaaaa atgtaagggg
       61 attcctatat ttttaagtat acttgttttt ccttttatac attttatggt attatcattt
      121 tattagttat tataaaacaa tcgttagaaa ggacgtcatt atgcaagtat acggaattga
      181 tgcggtgctt cgaatcgtga gtcatttggc gtttatctac ttagccttct ggtctttacg
      241 ttcgttacgc attgaaactt ttttcagacc aatgcataat acgcaaatta gaatggcaat
      301 cgtacttttt tcaatttttt tagggtacac tgcaagtaat tttttcttag aagttatcgc
      361 tttatgccga aatcttttcg ttagcctgta aattttaaaa aaaggtacta ttttaaaagc
      421 aaggatgtta taatagaaaa gattttgagt aagtgtatgc tcattggaat tttttggagg
      481 gaacaacatg gaagagatca tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat
      541 tgaaggagct aaaaatgctg ttttgcctat tttagctgct agtttgttag cagaagaagg
      601 tattacgaca ttagacaacg ttccgattct ttctgatgtc ttcactatga atcaagtgat
      661 tcgtcactta aacgtagatg tagattttga tgaacaaaag aatcaagtaa caatcgatgc
      721 ttcaagacaa ttagaaatcg aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat
      781 cgttgtgatg ggtccgctac ttgccagaaa tggacatgca aaagtagcta tgcctggtgg
      841 atgtgcgatt ggtaaacgac caatcgattt gcatttaaaa ggatttcaag ctttaggagc
      901 aaaaattatt caaaaaaacg gctatatcga agcaattgcg gatgaattga tcggtaacac
      961 gatttattta gatttcccta gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa
     1021 agcaaaagga acaaccatca ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc
     1081 aaacattttg aataaaatgg gcgctcaagt ctatggagct ggtactgaaa caatgcgtat
     1141 tgaaggcgtg gatcatttac atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc
     1201 aggtaccttt atggttgctg ctgcaatgac acaaggcaat gtgctgattg cagatgctat
     1261 ctctgaacat aaccgcccat tgatttctaa attgatcgaa atgggtgcag aaatcattga
     1321 agaagaaggc ggagtccgtg tcatcggacc aaaacatatc ttacctacag atgtaaaaac
     1381 gatgcctcat cctggtttcc ctacagatat gcaagctcaa atgacagcta tccaattagt
     1441 tgcagaaggt acaagtgtag tgactgaaac agtgtttgaa aatcgtttcc aacatttaga
     1501 agagatgcgc cgtatgaacg ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg
     1561 aaaccatgaa ttgcaaggtg cagaagtata tgcaacagac ttgcgggcag cagcagcatt
     1621 agttttagct ggcttgaaag caaatggaat cacacgtgtg cgtaacttga attatctaga
     1681 ccgtggatat tataatttcc atatcaaact acagcaatta ggtgcagacg ttgaacgtgt
     1741 agacatggat caaacttctg ctgaaaaaac agcacaaacg attgcttaag gaagtggctg
     1801 aatgagtaac caacgatata tcttattaac tttgataaag attttagtgg tgatcttatt
     1861 gcttatcctt ctttttgtgg ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa
     1921 tccatttaaa gtattccagc caagtttgtg gattcatatt cgagactttt ttcattgata
     1981 aataagaatc acagtggttc tagtcgatat gaagtgagcc atgaagcaga acatgacaaa
     2041 agaaaacaga agggaacgtt gacgttagtc gcgatccctt tttttagtaa aagaaaagaa
     2101 ggaatctaat tgatgaagaa gatattgatt tatttagtta gaggatatca gcgctttatt
     2161 tcgcctgcat ttccacctag ctgccgttat tacccaacct gttccaatta tatgataaaa
     2221 gcaattcaaa cccatggtgc actaaaaggg acggtaatgg ggactgcacg gatcttacgc
     2281 tgtcatcctt ttgtcaaagg cggtatcgat tatgtaccat tgacattcca attaacgcga
     2341 aataagaaag ataaaaaaga accagattat aaaaaggttg tgaaaaatac gagtagcact
     2401 gagcgataaa gaaaaagagg aaaattattg tcaaagtaac aactccgcaa ctacttatat
     2461 gggggacaaa aaatgatcga agaactaaac aagaaagatt taccatggtc cttgttatta
     2521 gatgcagatc ctgataaaga aaaagttcaa gtatatgttt ccagagggag tggctggata
     2581 tggaaagaaa aagaaaaaac aataggtgta ctgatctatg tggcaaggga aaaagaatac
     2641 gaaatcgtca atgtagcagt agccccaacg tatcaaggaa aggggattgg cggaaaatta
     2701 cttgagacag cttttcagaa gctttcacaa ctcgcttcca gtcaaactca gatcatcata
     2761 cgaaccggca gtactagcag tgcggcgtta catctctatc aaaaaatggg ctatgtggag
     2821 attggtcgag tcaaagacta ttttatccat cattactctg agcccatttt tgaaaatgga
     2881 gatcaattaa gggatcaagt aattttagca attcacttac ctaaaaatag ctttaactaa
     2941 taaactttca attcgagaaa atgctttcaa atgctaaata gatacattac aatagcttta
     3001 gcaaacaaaa aggagaatat ttttatggga atattagttt ttgattttgg tggatcggct
     3061 gtcaaatatg gttgttggga tggcaaagag attaaaggta agggacaatt tgcaacgcct
     3121 gaatcttggg aagaaatgaa agcgcaatta tttcaagttt ataaaaaaat actcgtttct
     3181 tttgaagtcg aaggaattgg aatcagctct cctggagtag tgaataatga gaaacaggtg
     3241 attgagggaa tcagtgcgat tccttatatt catggattta atattttccg agatttggaa
     3301 actctgtttc aactcccagt aacgattgaa aatgacgcca actgtgccgg aatggctgaa
     3361 ttttatgaag gtgctgcccg tgattatcaa gatgttgctt ttgttgtggc aggaactggg
     3421 attggcgggg cgttgttcac gaaaggacaa atcaataaag gggcacatct ttatggtgga
     3481 gaatttgggt tgatgttctt aaatggcgat aagacattca gtaaacttgg tacagcggta
     3541 cagatggctt ggcgttattg tgaacgcaaa ggtctcgata aaaattctta taccggaaaa
     3601 gatgtctttg aattagcaga aactggagat gcaatagcaa aagaagaagt tgagagtttt
     3661 tatacgtatc ttacaaaggg attattcagt attcaatttt cgtttgatcc agaagtgatc
     3721 gtattaggcg gcggtgtttc tgcaaaagaa gggctcattg atgagatcaa aagccgaatg
     3781 aagaagctga cagaacaatt cgatctgcat gatttcgaac cgcagatttt attatgtgaa
     3841 tacagaaacg atgctaatct tgtaggtgca gcagcaaact atctcgctgt acgtcaaggg
     3901 gagaattaag agctttagag aaggtgcagc ttgcataaaa gaagtgaatg aaatgaatag
     3961 gagattttta cctaaggaat gttttatttg ctgcagtatg ataaaatatc tgagaggtga
     4021 aaatgatgtc aaaaaaaaat aaagatgttg aagtgcgtat agaagaagtg aaaaaagata
     4081 tcaaaggtaa gaattatgaa gtcactcagt tatttatagg gaaaaaaatg attggtgaga
     4141 tcttagccta tgggccaaaa gaatatgaaa ttttctttgg tgaagaagat tttggaaaag
     4201 aaaaaagtct tgaaaatgcg atagaaacag tgattcgaca ctggaatctg cacgaataaa
     4261 aaaatatcaa aaaaactttc aataaagggt tgcaaaaatc tggaaaatta ggtattataa
     4321 ctaagtcagg ttgatacaaa cctttcggag gagtagcgaa gtggctaaac gcgacggact
     4381 gtaaatccgt tccttagggt tcagtggttc gaatccactc tcctccattt cattggggta
     4441 tagccaagcg gtaaggcaag ggactttgac tccctcatgc gttggttcga atccagctac
     4501 cccagtatac cacaatcgta agctctcgtt agttcgagag ctcttttttt tgctttgcta
     4561 ccttgttcct tgaagattct gctgatttaa gatagagtta agttaactac aagaattgga
     4621 gggatccaca tgaaatggaa agaaaaagta gacgaagcag ctgaaaagtt atatgatttg
     4681 gtcaaatcag aaaaatacaa tattgaagtg aatattccga aaaaaggcgg aaaagcggtc
     4741 caggtaaaat caaaacgccc aacgaatcat acaaaaaaat ggatggctaa aaaccgctaa
     4801 taataacaaa agatccataa gtcggcattt tcatcgattt tagggtcttt tgttattttc
     4861 taggttgaac atatctaaaa aattgtttct gacaatagga gtaggaagaa cgatgtgtgt
     4921 ctaaatcctc tgttgatgga tagaaacctt tatctttttc agttgtgaat caggtatata
     4981 ccagttataa tagaagaaac gaaaggacgg gtgtacgtta tgtcgaatca attacctgta
     5041 tatatacaga ttcatgacca attaaaaaat gaaatcgaac aaggtatatg gaaagtcgga
     5101 gaccgtcttc cttcagaaag agaattagca gtgaaattca atgtcagccg tatgacgttg
     5161 agacaagcaa ttcaaaactt ggcagatgaa ggaatcttag aaagaaagat cggttcgggt
     5221 acatatgttg cgcgagaaaa agtacaagaa aaaatgtctg gtgcaacaag ttttacagaa
     5281 attatggaat cgcaaaatcg agtaccgtct agtcggacaa tctcttattt cttgacttcg
     5341 ccaagctcaa gtgaaatgga aaaactaaac ttgagtaaag atgacacgat cttgcgaatg
     5401 gaacgtattc gtttcgcaga tgatgtccct atttgttttg aagttgccag tgtgccacaa
     5461 aagctgatcg atggctacag caaagcagag attactgaat cgttgtatcg cacgctagaa
     5521 gaaaaaggcg ggaaaaagat cggtccagcg aatcaaacga ttactgctgt gttggcttct
     5581 gagaaaattg ctgactattt agaggtaaaa cgaggagatg cagttcttcg tttgcgccag
     5641 atttcttatt ttgaagacgg tacgccgttt gagtatgtgc gtacgcaata cgtaggaaat
     5701 cgttttgagt tttatttgga aaaataaaaa acaggtgatc cctgattcct tacttagaaa
     5761 taaaagagaa tcagaatcac ctgtttttat atatgatcag ttaccagttg ctcgtgataa
     5821 gaaacaggac cctcttcaaa ttggacttgt ccgttcagca gctcaatgat ggattcttta
     5881 aagtggtcag cacttttttc atctacagca cagatgacgc taacttgatc agtatattgg
     5941 atttctttta taaaaaaatg ttggagttca agataattct gtaattttcc tagttgcgga
     6001 tacgtgatca tcacagagat ctcctgttgc aaggtacctt ctacgattcc aatgtcagac
     6061 aatgcttgag agacagcatg agtgtatgct cgtattaatc cgcctgtgcc gagtttcgtt
     6121 cctccaaaat atcgagtgac tactgcagct acattgatca gttgattttt tttgaggact
     6181 tccaacatcg gtacgccagc cgttccactt ggttctccat catcgctgct tcgctggatt
     6241 tcattttttt ctccaatgat gaaggcgctg cagttatggt tcgctttcca atgttctttt
     6301 tttatttctt gaatgaaact ttttgcttgg tcctctgatt ctattcgtct aagagaacag
     6361 ataaagcgtg attttttgat ctcaatctca ctttgtccat tttcttttat tgtacgatag
     6421 cgctcgatca tcttgtcacc tctttttttc attttcttta ttcagtataa ataaagttct
     6481 aggttgtttc aacactgtaa cggttgcgca atcgctgtag tatgcgataa taaagacaaa
     6541 ggtaggtgga tctggggatg caggaacgaa tgaaaaagta cgatgcaatt acgcattatt
     6601 tgaagaacaa tggcggctct caagtaacgt tgacctttac ccaatttgac gaactgctgt
     6661 ttcccagtaa cggacttccc aaaacagcaa gggaaagtac agattggtgg gcaaatgatt
     6721 ataaacaccc tgaaaaaggt gcctatggct ggataaatgc aggctatgaa gtagttgtca
     6781 tcaatttgga caaagaatat gtcgtgttca ataaattagt gaaatctagt tggctatttg
     6841 attaaataaa ccgcgtttag aaaacgttga caaatttctt tctttttata taatagagta
     6901 acagacgcga agaaaacgtt tgcgtagttt gttttaaaaa ggagagaaaa gtatggctaa
     6961 aaaaacaatc atgcttgtat gttcggctgg tatgagcaca agtttactag taacaaaaat
     7021 gcaaaaagct gctgaagcaa aaggaatcga ctcggatatt ttcgctgtgt ccgcttctga
     7081 cgcagataac aatcttgcaa ataaagatgt agatgtactg ttattaggac ctcaagttcg
     7141 ctttatgaaa gctgattttg aaaaacgtct ggaaccaaaa ggcattcctt tagacgtcat
     7201 caatatggct gattatggta tgatgaatgg cgaaaaagtg ttagatcaag caatcacgct
     7261 cataggtgaa taaatagaac agtataacta tcgaacaagc gatccaaaaa atagatcgct
     7321 tgttttttcg tatatttttt tagggcgaat aatatgaatc atttttgggg tagaaggctt
     7381 ctgacgagag atattaaagc aatagactgt gaggaggtaa atttaccaaa ttatgagaag
     7441 atcgcagcag tggaacgttt gggcaactgg attctttgtc atcgctgtgg aatgaggacg
     7501 tccgtcattg atggacagct tgcagattac ggttattttt gtacacattg tctatattta
     7561 gggcgatgcg acactcaaca agatttatat ttgtttgatc agcctgaagc aaaacctcga
     7621 gaagtcgtct ttttttggac aggacagctt actgaaaaac aaacggaaat cgcaaagaga
     7681 atactttgcc attcagaaaa aagacatcat cttatttggg ctgtaacagg tgcagggaaa
     7741 acagagatgc tttatcctat tttagtggaa actttaaaag ctggaggaag agtagcgatt
     7801 tgtacaccgc gagtcgatgt ttgtaatgaa ttgtttttac gctatcgtta tgtttttcca
     7861 gaagaaaaaa tcacgttgct ccatgggaat acagcaacgg cttatacctt tacttctttt
     7921 gttatctgta cgattcatca gctataccgt ttttatcggt cctttgattt aatagtgatc
     7981 gatgagatcg atgcgtttcc ttataacggg gatgcaggat tagctcatgc tgtgcaaaca
     8041 gcgattaaac cagacggacg attcatctac ttaagtgcca cacctgataa aaagttacta
     8101 aaggaaatca agcagacgtt tgacatccac aaacttgctt tgcgctttca tcggcgttta
     8161 cttcctgaac ccgtcttggt tttcatgaat aattggcgac aaaaatgcaa gaacaagaag
     8221 aagataaaat ctttggtgcg gatcattcat caattaatta aagaaaacca tatcctttta
     8281 ttttgtccaa gtatcacgat gatgcatcgt ttgaaagcca cacttcaaga ggctcttccg
     8341 agatatgaga tcacagatgt ttcttcaaga gacaatcagc gggcggaaaa agtccagaaa
     8401 atgcgagaag caaaatatga catactgtta accacgatga tccttgaacg tggagtcaca
     8461 tttgaaagaa tatccgttat cgttttagga gcggatcatc cggtttttac taaatcttct
     8521 ttggtacaaa tcgcaggaag agccgatcgt aaagggcctt atacgaacag tcaagtatac
     8581 tttttctatg aagaaaaaac tgcagctatt cgaaaagctt gtcaggaaat caaagaaatg
     8641 aatgaagaag ggaagaagat gctgtgaggt gcagttgctg cagtaaagat atcgtccaaa
     8701 atttaacatt cagcgagatt attggtgttt cgaaatacag attacctcag acttgttcag
     8761 tttgtcgcgc acaatttcat ttattggaaa caagaggctg ttctggctgt caaaaaccaa
     8821 tccatttcaa tctagagagt acgaaaaaga aagtaaagtc tattcccgtt tactgcagtg
     8881 actgtcaaga atggaagaag aaatatccga cctatgattt tcagcaccgt gccttttttg
     8941 tgtacgatga aaatatgaag gcctggctca atcaatacaa gtttatggga gatatccgac
     9001 tagcaggcac ctttgcatat aaatggacgc agataaaaaa agaatacccg acatacatct
     9061 attgtccaat acctttatct gaaaaaaggc gaacagaaag aggattcaat caagtaagcg
     9121 agatgttgaa agcagcaaga attccttttc agatgctact caaaagagag aagcatctgc
     9181 tggctcaagc aaaaaacacg cgaaaggaaa gattggagat gcctcaacct tttgaacttg
     9241 cagtcacctt ttctaagata caaaataaaa aaatattgat tattgatgat gtctatacaa
     9301 caggacagac tcttttccac gcagcagatt gtttgcaggt cgcacagccg aaaatcatcc
     9361 gaaccatttc tttagcaaga tgaattagat gtggaaattt gatagcgatt taatttgcaa
     9421 ataacctaga gttcgatata atagaattaa gaagagggaa agagtggtcc ttctccttct
     9481 tgattgtggt agacgaaagg ggtaattttt atgtttagat ataatgtacg cggcgaaaat
     9541 atcgaagtaa ctgaagcgat tcgcgactac gttgagaaaa aagtaggcaa actagaacgc
     9601 tatttcagtg acgcacccga tgcaacggcg catgtgaact taaaagttta tacagaaaaa
     9661 acagcaaaag tggaagtaac gatcccatta ccttatcttg tattacgtgc cgaagaaact
     9721 tctccagatt tatacgcaag tatcgatttg gttgttgaca aattggaacg ccaaatccgc
     9781 aaattcaaaa caaaaattaa tcgtaaatcc cgtgaaacgg gcatgaatac tgcagatgct
     9841 actgctttat tcaatgaaga ggacgaaaac gaaaatgatt ctgaattaga tatcgtacgt
     9901 acgaaacgcc tttcattgaa accaatggat agtgaagaag cagtcttaca aatgaacatg
     9961 ctgggacata acttctttat ttttgaagat gcagaaacaa atggtacaag tatcgtttac
    10021 cgccgtaaag atggaaaata cggactgatt gaaacagact gatcagcctt acttaaataa
    10081 caa
//
LOCUS       DARBDV010000098         9869 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000098 DARBDV010000000
VERSION     DARBDV010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.100"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            205..684
                     /locus_tag="SCM56_002431"
     CDS             205..684
                     /locus_tag="SCM56_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111172.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            717..2945
                     /locus_tag="SCM56_002432"
     CDS             717..2945
                     /locus_tag="SCM56_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1111173.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3074..4717
                     /locus_tag="SCM56_002433"
     CDS             3074..4717
                     /locus_tag="SCM56_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEG1111174.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4790..5335)
                     /locus_tag="SCM56_002434"
     CDS             complement(4790..5335)
                     /locus_tag="SCM56_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEG1111175.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5358..5843)
                     /locus_tag="SCM56_002435"
     CDS             complement(5358..5843)
                     /locus_tag="SCM56_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEG1111176.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5833..6645)
                     /locus_tag="SCM56_002436"
     CDS             complement(5833..6645)
                     /locus_tag="SCM56_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HEG1111177.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6784..8316)
                     /locus_tag="SCM56_002437"
     CDS             complement(6784..8316)
                     /locus_tag="SCM56_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEG1111178.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8425..9456)
                     /locus_tag="SCM56_002438"
     CDS             complement(8425..9456)
                     /locus_tag="SCM56_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1111179.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3284 a         1832 c         1898 g         2855 t
ORIGIN      
        1 aaatattcca acatcatctt ggactttcta catgttacta atgagcgtgt aaaagaacga
       61 gatggtgttt ttatttctat caatgaagct ctttttcttt gcaatcctca cttcaatcct
      121 ctataatgtc gcatatagtt tagtaaatga gtaaactact aatttactta tttactaaaa
      181 ataaaaggat gaggagttaa aataatggaa ataccaacaa atttaaaaca taaaccagta
      241 atcgttgtag aagactatga taaagttgat ggaagaaatg cattaaatat ggatgcaaaa
      301 gggctttcac tgggccttgc tcagtggaat gatcgtggaa aagtcgatat ctcaggcaag
      361 gtatggcgac atactggaga aaaatggtca cgacagtcgg aagaattacc aatcacacgt
      421 attttagatt tagcgatctt agctgcgcaa ggaagtttat acttccaaga tgcttatcgt
      481 tatgaaaaat tttatgatcc agaaaatcca gtcgtagaca tcattggatt acaagggaac
      541 cgaatgacgg tagaagtaga taccaaaaat ccaaagatcg atgaagacat cattttgtat
      601 tacgatacac tacaaaaaga cggagaactg attggggaac gattccgtat tttaaaacgt
      661 cttttagatg agctaggata ttaatagaca gagatgatca aaaggggaag agaagcatgg
      721 agaatttctc actaacagaa aaccgaacct tttcacggct agaaaagcaa atgatctgga
      781 agaaaccgaa aagccatcgg acaagtgcag aagaatggcg gatcgcctcc gaaatcaaag
      841 ggaattggca agatcctgaa atgaaaatca gtaacgtact tttggaagga gatgccgggt
      901 cggggaagac acaattagcc aaagcactat ctgctgatct gcaacttccc tatacaaaag
      961 tgacctgttt tgcagatatg gacaaatcag atgttttcgg agccttatta cctgtagtag
     1021 attctaataa ggaacaagac caagaattac tagaagctat ttaccaaaca gatacacttg
     1081 aagctgtact gacactgatc cagaaccatt acggcattga tcctttttct gctaaagaaa
     1141 aattagcaga gctagttcaa aggatcgaag ctgattatcc agatgcaatc cattacagct
     1201 attacccttc tgaaatcgtt cgagcgattg aaaaaggcta tttattagag attcaagagc
     1261 cgactgtcat ccgagatgcc tctgttcttg tggcacttaa ctcagcgctc gaaccaaatg
     1321 ggatgttgaa tttaccgaca ggaatcattc gtcgccatcc agattgcgtc attgtgatca
     1381 ctacaaatcg aaattaccaa ggaaatcgcc cattaaatga atctttaaga gatcggatgc
     1441 aacatgcgga aaaaatggat cttcctgcct tagaagtcat ggcagaacgt gcgatggcga
     1501 aaactatgat ccaaaaacaa gaacttctct taaagatggc agagattatc cgtttgttgg
     1561 atgaaactgc aaaagctaac gccatcaaag gtgtagcagg aatgcgttct tatttttatt
     1621 gggcaaatac ctataagcaa ggacaagatt tactccagtc gatttatcca aaagttttgt
     1681 ataaattatc cactgattca gatgaattgg caattcttga acaagctttg tctgacagtg
     1741 gattgctact ggaacttcag gacatattga gaaaagaaaa atggggtcat cctcaatctg
     1801 agcatacaaa agaaagaacc attagcgccg aagaagcaaa tgaacgaaac atcactgaaa
     1861 atgctgaaga aattctgcgc ttagatgaaa agaaatcgtt gtctgctgaa gaaccaaatg
     1921 agggagaaga gaaacaagaa gaaacaatag aaaaaaacga gattcctcct caaatgcaaa
     1981 aagaacgcag tagcgatatc gaaggaaaaa atcaaaaaga acaacaaaca ggagatgatt
     2041 ctcaagaaat gtctgcttct gacatggaag cacatgataa ggaaatcaag aagcaactga
     2101 ataaagaagc gcgacaaatc atgaaggaaa cgattcatga aaaagaaggt ctcatcgttc
     2161 accgaccagc tttccaagga aaaacgccgg aagcacgtga gctacacatg caagtaatgc
     2221 caatagtaga atcacttagc cggcaaattt tagagttact ggataatgaa caaacagaga
     2281 cgtaccaaaa aggaaaatac gaaggtcagc ggttcaatgc aagcaaagta gcatatggtg
     2341 atttgagaaa ttttgacaaa aagaacccgc cacatgagca accttcttta gcggtagctg
     2401 tacggatcga tgaatctggg tcaatgatac gggatgatcg gatcgaagcg gcaaaaaaag
     2461 cagcgattgc cattgccgaa ttttctaaga aagtcgatat cccattgttg atttacggag
     2521 atacagcaga ccgttcgtca agagaaaaga cttctttgtt ttcttataaa gaatttgaag
     2581 acggatttca atggttaaat gaaaaattag tcactatgaa accaaggcaa aacaatcgtg
     2641 atggtgctgc acttcgttta gcagcagaaa aattaaacag acaatccgca acaaccaaac
     2701 tgttgcttaa tattagtgac ggtcagccaa aagcattgcc ggattataca ggcaaaaaag
     2761 caaaagaaga tatccagtct gttttgaaag aatatgaacg tcaaggaatc ctatttgtct
     2821 ccgcagctat tggccaagac aaagaagaaa tcaagtcgat ctacggtgaa agtcgttttg
     2881 tagatattac tgacttgaat gaatttccca agcaaatgat tcaactgatt gcccgctatc
     2941 tatgataaaa taaagctcaa cgaacgcact tagcagattc gttgagcttt tcgtttctag
     3001 ttaccggtaa caaggatact tctaaaatag aatcggttat aatcaagagg gaccgttaaa
     3061 ggagtgaaac gatatgatac aagaacaaaa atggtggaaa gaagcagtag gctatcaaat
     3121 ctatcctgct agtttcaaag atagcaatgg cgatggtcaa ggagacatca atgggatccg
     3181 tgaaaaactt aattatttga aagatcttgg tatcggcttt atctggatca atccgatcta
     3241 tcaatctcca tttgtcgata atggttatga cgtaagtgac tatcaagata tagaagagag
     3301 attcggaacg atggaagatt ttgatttatt gctaaaagaa gcacatgaat tagggatcaa
     3361 agtgattatg gatcttgtca tcaaccatag ttcggatcag catcaatggt ttgaagaatc
     3421 gaaaaaatct aaagaaagcc cgtatcgcga ttattacatt tgggtcgacg gggtagatgg
     3481 caaagaaccg aataattgga catcgatttt cggcggttct gcttgggagt attctcatga
     3541 aactggacag tattatctgc atgtttttgc aaaagaacaa ccagacttaa attgggaaag
     3601 cgagaaattg aaagaagaac tattcaatat gatccgatgg tggttggata aagggataga
     3661 tggatttcgt ttagatgcga tttctcatgt aaaaaaagat gaatattcag taaaagccac
     3721 agagaaccct ttctcaccct ttcaaaatgt ctcaggaatc gaagaacatt tgaccgattt
     3781 gaaacatgtt tttgaagagt acgatatcat gactgtcgga gaagcaagtg gtgtcactgc
     3841 agaagaagga ccacaatggg tagggaaaga cggttatttc gatatgatat tcgaatttga
     3901 tcatatccat atctggcagc aagaaaaaga agggcaatta gatgtcctta aattgaaaca
     3961 tgcattaagc gcttggcaaa cttcattgga tggtatcgga tggaacgctt tatatatgga
     4021 aaaccatgat gttcctcgtg ccgtttcagt gtttggagat actcgtcccg atttttgggc
     4081 tatgtctgca aaagcaatcg ctatgatgta tttctttttg caaggtacgc catttattta
     4141 tcaaggacag gaaatcggaa tgacgaatat gccctttgaa tcgatcgatc aagttgatgc
     4201 agtagattcg aaaagacttt ataaaaggct gcttgcggaa ggtaagacaa gagaagaagc
     4261 attggatatc attcgtgaaa caacgagaga caacagcagg acacccatgc aatggacttc
     4321 ggaacaatat gctggttttt ctacacatga accttggctg atcactaatc caaataccaa
     4381 aacgatcaat gtagaacaac aagaatatga accagagtcg gtcctgcaat tctataagaa
     4441 catgatccgc attagacaaa ccaataaagg tctgatatac ggttcttata aagaatatct
     4501 tcatgaacat ccacaacttt atgtctatga aagatattta gaagatgaac aatatcttat
     4561 tatggtaaac ttaacagaat cacttgctga ctatgaactt ccaaaagaag cagatcagtc
     4621 ctggacatta ctcttgtcaa attcctctag cggagaattt gaagctaaag gcatactagc
     4681 accttatgaa gctcggttat ataaaacaaa caagtaaaac ttcctcgtta aagttcatta
     4741 gttggacttt gcgaggaagt ttttcagatt gctctgtcaa ttactcattt caataattcg
     4801 ctctaggacc accgatatat ttaggacgag agcgaagggc tgtcacgtga gcttgtccaa
     4861 gggttttcaa acgaggtctg acaggtactt ggacatgctt gatatgcata ccgattgatg
     4921 tgtcaccgat atcgataccg gcttgtccaa ctacgtgttc cacttcgaca ggatcattga
     4981 actggtcaaa agcagcaaca gaacaagcac ctccagcatg tagtgcagga acaacagaga
     5041 caatttcgaa attttttgct tctgccactg ttcgttctac tactaatgct cggttcaaat
     5101 gttcacaacc ttggacagct agcgcaattt ctttaggatc caacaactct tttaacgtac
     5161 ggatgatcca ttgaccaact tccgcactgg aattttttcc aatatgtcct ccaacgactt
     5221 cgcttgtgct gcatcctaaa acaaaaagat cacctttttt caagtgtgcc tcagccaata
     5281 tgtcattaac tatttcagtc aattggtctt ttaattcttt ttctgacaac ttcattttct
     5341 aaaaccttct ttttcaatta atattgcttc cgtgcttgaa tacgttgaag acttgcagtt
     5401 ataggaaagg caagtcccat tccaatcaga ttttggaaga tgtttcctgg tatagaagcc
     5461 actccagccg gccaatcata taaaatccaa ccggcgatac catagccgat gatcataact
     5521 aagcttccta ataaaagccc taatccccac atacgagtag aagatttgct gaaagaacca
     5581 gcaacatatc cttgtaaccc gtggatcact aatgaaaaaa ctgcccactg cggatatcct
     5641 gaaatcaaat cgatcaatag accactggaa gcaccaacaa ctaatccacc cgttggacca
     5701 taaagaaaag cagcggtata gatcccggcc tcgcataatg tcacaaatcc attcgtagcc
     5761 ggtacaggga tcaaaattgt taatgaaagg gcaactgtca acgcaatcag catagcatag
     5821 atcgttaatt gcttagtttt catttctcat tcctcctttt tgaataccgc cagaccagac
     5881 acttccggta tgatcgttca tcagcacgcc attagaaata caatgggcaa caaatttttt
     5941 actttctgct actgctttgg taaaatctgc ccctaacaca agattcgctg ttattgcaga
     6001 agaaaaggca caaccggccc cgtttactgt tgtagtgtcg atttttttat gagaaaatac
     6061 ctcataatga tcaattgaat agagaagatc cacagcttca tctccagcca tccttgtgcc
     6121 gcctttgatc acaatgtttt ttgttcctga agcagtgagt tctttagcga gatggatcat
     6181 ttctgttaac gaagagggaa gagcttgacc agaaagtaaa gcagcttctg ccaaattagg
     6241 cgtaatcatc gtaacaagtg gaaaaagttc ttcgatgagc tgcgaaatat acaattgatt
     6301 gatcacagct tcagtttctt tgaatgccaa aacagggtct aaaatgattg ggatattttg
     6361 ttttgttttt atgaagtctc gaacaaccat gattgcttca cttgaattca ataacccaat
     6421 tttgatacca tctaatgaag tcattttatc aatcgaaact aactgtcgtt taagcaattc
     6481 caaagatata gattccagca taaatcgctc attaatatct agtgccccta tagaagtcaa
     6541 ggcgcttagt ccgaatagac caaaattctc aaaagttttt aaatctgttt ggattcctcc
     6601 accagcaaat ggatcagaac cgccaattgt caagattctt ttcataacat tttcctccat
     6661 gaataagagt ataacgaaat tttcaaaaaa gaaaaacagc caattggcat atttttatct
     6721 catccaattt aaaaaaacaa aaagaaaaga aagaccaaaa tggatctttc tttccttaaa
     6781 tagttaggac ttttgtgatt ttaattcttt ctcgatttga agccgttgca ctttcattgt
     6841 agctgttcgc gggattgcat caaaatcacg gatgataggg acgttcaagc gaggaagttc
     6901 atggacttgc tcccaccatc gattccaatc catttcttta tcaggagcta aagcaataat
     6961 cggttgtgga gaatgatttt tatcccttac aatcactact tctgccaaaa aatctaacga
     7021 atctaataaa aagtcttcaa tagctaatgt actattgatc gtttcgatca aatccacttg
     7081 ccgatctttc aaaaataggt ggccatgctt atccataaaa ccgtaatctc cgctatccca
     7141 ccaatcgcca taaacatttt cctggaaccg tgcatcttcc ttgtaataag tcaaagcccg
     7201 tcctttagac agtaattgta tatgaccatc tgtgttttca ggtaggacat tcccaactga
     7261 atctgtaatc cgagcgcttg tcatgtcttc cagtccaacg cccatatctc ttgcatcact
     7321 tgttttcaaa ctctctagtg tatgagcctt aagaatcatt ggtccgcatt cactttggcc
     7381 atatacttga agaaataccg catcctgtgc catcgacgtt cgcaaaaatg tcttcattgt
     7441 ctggttgttt atagcatcaa aagtggagtg atagtatcgg atacccgaaa aagcttctgg
     7501 atgctcctta gcagtaaaac gccactgaac aaagttattc ggatgggtct ctagtgcaat
     7561 tggccggtga gcttccaaca tatgaacaac ttttgaactt tgtgcgtttg ctaaaggcat
     7621 cataggaaat cccatggaca tcaaagaaga gataccaata ttgaatctgg aatgcactgg
     7681 agagatgtga aaagcgatta atttcttttc cgaaattttt gtgaaaactg ttttttgcca
     7741 tttcgtccgc catcccatag aatgagcaga atgacaaata agtttaggga taccagttgt
     7801 accagaagta tgggtcatat aagaaatttc atttttgtct aaaaatgatt gagaaacaga
     7861 caaagcaggt tgttccatta aataaagaat aggaatctgt tttgattttg ttccatttga
     7921 aatttctctc actcgctttt ttgtctcatc atcaaataaa atgaaagggt cttctaaacg
     7981 atcgataaat acttccattg ttgttgttgg tagatgataa gaagtcatga caggcactgc
     8041 accaaggtaa gacacagaaa cagctagaag gtatgtgtca aaagcggaac tcttataaat
     8101 aataactttg tctccggatt tcactccaag atgagccaat tgataagccc gtttcaacac
     8161 actttcgcag cattttttgt aagttgtttc aagtcctaat tctgggaaag ctgaaagtgg
     8221 ctcatcaaag ataatcgggg tctcggaaaa gttagctgca gcttcttgaa aattcgtaaa
     8281 taagttcaaa ggggaatacg ttatggtctc catcattcac actccattat cttttgttaa
     8341 gtaatataca aattcaagat aatcgtaaca taatccttta tataataaca tagtttattg
     8401 tgaaaaaaag atcatacatt tgttttatat ggaagaaacc cgaacgaatg ccgctaattc
     8461 gtgataagaa aaatcaacca tcttttccca gatatcttga aaagaatggg atgagaggta
     8521 tcgattacca cataaataat ttaccgcgtc agcatcatct aaatatagtt ctgcataata
     8581 acgatatgtt tttctgcata gttctggcga tacgttttta tatactgtaa ttttgctcaa
     8641 aatttggcga atagtccgag gagaaatttg ctgatcatgt gaggaacgaa aaacaaaaga
     8701 attttcgtcc gtattttgtg ctgttaactt aaaatagttc tccagaaatg gcatcgcttc
     8761 ttttggtatt ggtactttat tcagtctgtt ttcttttcta ttcagattaa gaatctgctt
     8821 ggtcaaagat acatctttac gtttaagttc tactacttca tggatcttta atccgcaata
     8881 cagaagaatg gcagtaattg caaggttcct cggtctattc ttttcttcaa agctagcttc
     8941 tctgcttgtt tgaaagcctt caatcgggat aaacaaatgc tcaaaatctt tgaaaagttc
     9001 tgcatcgata tatcgcggag ctgctttggt tgtttccggt aaacttgtac ccaaaagaga
     9061 aatctcttct tcaatcagtc ctttttgata caaaaatcgt aaaaaatgat ttaatgaata
     9121 ttgcttcctt gaaatcccgt aatagctatt ttcaaacaat tgctccgttt cttggttcgc
     9181 tgtagtttgg aacgaacgag tatgtcgata gattttagct aagaaattct gataatcttg
     9241 tttttctaaa tggataatca gctgatcatc cacttcttca gaaaatactt ttctcaaata
     9301 atcagtaaac agatgaaaat cttttgcgta ttcatagcga ctgcctaatg aaagggaaga
     9361 acgattttct tctaaaaact gttgaaagac ggaagggagc aagtgtgtac gttgctttaa
     9421 cttttcttgc agttgttttc tagttttgct gtccattaca aattccctcc aatagtgttc
     9481 attttctata ttttaccgca tttcatcaaa tgaagataga cagtttgtct tgatcttagg
     9541 aagcaaattt ttaactaagt actagctaaa agatatgaag taagaagata catttttagt
     9601 tattttatta gtgcagtcat tcttttgatc ttttaattga tttatcctct ttttagctat
     9661 gtggttactt catgagcaat gaaatggatt tggttcgttc taatggcagg aggctctttc
     9721 ttttatttcc gaaaaattag aaaacatttt ttcagaatcg attggctctc catttattca
     9781 ataatggttt gtctaacgat attgttggga taattcttaa agaagtatta gaaatagttg
     9841 gtacttcatt actttacatg aagatttat
//
LOCUS       DARBDV010000099         9830 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000099 DARBDV010000000
VERSION     DARBDV010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.101"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..205)
                     /locus_tag="SCM56_002439"
     CDS             complement(<1..205)
                     /locus_tag="SCM56_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111180.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(221..835)
                     /locus_tag="SCM56_002440"
     CDS             complement(221..835)
                     /locus_tag="SCM56_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEG1111181.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(982..1665)
                     /locus_tag="SCM56_002441"
     CDS             complement(982..1665)
                     /locus_tag="SCM56_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEG1111182.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1774..2232)
                     /locus_tag="SCM56_002442"
     CDS             complement(1774..2232)
                     /locus_tag="SCM56_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEG1111183.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2316..4079)
                     /locus_tag="SCM56_002443"
     CDS             complement(2316..4079)
                     /locus_tag="SCM56_002443"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEG1111184.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4300..5760)
                     /locus_tag="SCM56_002444"
     CDS             complement(4300..5760)
                     /locus_tag="SCM56_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111185.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5968..6894)
                     /gene="glsA"
                     /locus_tag="SCM56_002445"
     CDS             complement(5968..6894)
                     /gene="glsA"
                     /locus_tag="SCM56_002445"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HEG1111186.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6917..8344)
                     /locus_tag="SCM56_002446"
     CDS             complement(6917..8344)
                     /locus_tag="SCM56_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1111187.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8375..9589)
                     /locus_tag="SCM56_002447"
     CDS             complement(8375..9589)
                     /locus_tag="SCM56_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEG1111188.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         2917 a         2041 c         1628 g         3244 t
ORIGIN      
        1 atttgttaaa gtaattaaac aatccgctaa tcgtttgatc tggcatttct atttccttac
       61 catctaaagt aacagtagta acgacaaaat ggtcttgcaa tggcggaact tctaagtact
      121 gcccttgcag aaaatcattc actgtttcat attctttttt catttctttt gaatctgtcg
      181 atggtttata gcttattttt aacataacta gtgctccttt ttattcggga cgtgcaatcg
      241 tgaaaatttc tcctattgct gcataatcat ttcctgccaa acggcttaca gcattcaatt
      301 ttcttggatc gatccttcct gtcttatcat catagactgt ttcatttaaa tgatatttct
      361 gtacttctag caaaaataaa tcagctgtta ctttattttt attccgtatc tcgacatgtt
      421 catataagga tgtttcaaaa cgaacttttg cttctgtcaa tgctggtact gaaatgatta
      481 ctgaatcagt taaagtcaag tcagtcaggt acagttcgct ttcctcatta ggcagattgg
      541 ctgctgtttt atttacttct tctacgttgt cctcatcaag gatatggata acagcttctc
      601 ttgtctcaag aatattcttt gctgtgtctt ttactgcatc ttgttttctc tggatcgcca
      661 aggataagat gggtcgattc gaactgacga tattgaagta actgaagggt gcgatattca
      721 ctatatcttt tgctgattta gtcgttacta ctgctactgg tctagggatg actgaaccaa
      781 tcagcaactt gtaattttca cgttctgtca attctgaagg actccgttca aacatggttt
      841 cgccttcttt ctataaaatt cgttaaaaag cagatcttga agacaaaatg aaaagtaatg
      901 cttttttcat cttcttagaa tctgctttaa actatctatc aatttttctt ttttgtcaag
      961 aatctgtgcc gtgcgtgttt tttagttatt tgctttactt gcggcttcga catatctttt
     1021 agtgatcaat tgcggacggg tgataataga tccaactacg actgaaaaag cacctaattc
     1081 atatgctttc tctacttgtt caggagagtc agtatgtcct tccataatta ctgggatatc
     1141 gacttgttct atgatttgat tcaataaatc aaaatcaggt gtctgcgtct ttcgtgaata
     1201 ttctgtatag cctgctaaag tcgtagacaa aagatcaaaa ccatcttctt gcgcttgaag
     1261 cgcttcctca aaagtcgaaa tatctgccat agctaaccga ccgtgttcgt gaatcatttg
     1321 gacaagttca gcagttgttt tttctgtcgg acgtttacga gaagttgcat ccagtgcaat
     1381 gatttctgct tgcgtctcca aaagttcttc cacttctttt tctgtcgcag taataaacac
     1441 ttctgaccca tcgtaatctc ttttgataat ccctattacc ggtaaatcaa cgattcgctt
     1501 gatttcattg atatcttctg tcgtatttgc tcgaatacca actgctccgc cttcttttgc
     1561 agccaaagcc attctcccca taataaaaga actgtgtagc ggctcgtttt ccaaagcttg
     1621 acaagaaacg acgactccgc ctttgatttg ttccagtgta tccacaatat ctcctccagt
     1681 aatgttatat aaaaatacag cttcatataa aaatgtaacc cttttctatt caggagacaa
     1741 taatgagcta tttctttcag cagacctttc ttactaaaag aaaattactt caaattcttc
     1801 gtatacgacc agcttctctt ctgtaaattc tatgttcaac gcttctttat aagcatgttt
     1861 aaaaaactca gcatggactt tccgccaata agctaatgtc cgatcccctt ctccttcttt
     1921 ccaagcatgc tctttcgtga cttcactaaa aggcaagatg gtcacattta tatttttgat
     1981 gatacaaact ggttcatttt ttccattcag gaccacgtct aattgaccaa cttttggtaa
     2041 aggctcattc tcttgtttat acagatcgta accacttgat gtaccagtct ttatcttttt
     2101 tacaactagt tcgctcaact cgtctgccat tttttctgta tccccaaatg aaaagaaacc
     2161 taatttttct gattgataac taggatattt ttcttggaac tttttgacat attcttctac
     2221 ttgctgatac ataacatcca cccctttgct ttttataatg aaaaaaccat ccagatacta
     2281 gtataaacaa ttatctggat ggtgagcata atgaattatt gaatattcaa gacacgcaga
     2341 ctatcttcaa cgatttgttt tggtgttaaa tgataacgtt cataaagctg ccctacagga
     2401 acgctatccg tgaattcttt ctctgcacca aaatttaata cgttcattga agaatcacca
     2461 taataacgac tgatattttc gccaaatcct cctgacaaac taccatcttc taatgtgatg
     2521 actaattcgt gtgtttcttt taatttatct aatgtttcta cgtcattttc tgtgatcgtt
     2581 cctggattga tcaacgttgc ttcgattcct ttttttctaa aagcttttac tgtttctttt
     2641 cctaattcaa gaaaaccgcc taaagctaaa attgctactt ttcctccatt atttactgtt
     2701 tgataggctg gaatactata atcatttgcg atagctgtac catgttctac accatgtgta
     2761 ggaattcgga ttgctacagg ctgcgctgtt tgttcgaacg cccaatctag catagagatc
     2821 atatcctctt ttgttgctgg agcaagatat ttgatgttcg ggatacttga taagtaagga
     2881 atgtcaaatg ttccttggtg tgttcggtca ctagctgaga tcgttccgcc accgactaaa
     2941 ataatcactg gattctcgtt gatcgctaaa tcatgtgata attgatcata tgcccgttgc
     3001 aagaaagtac ttgattggaa gacgactgga cgtactccct gagaagccaa agcagaagcg
     3061 aaagtgatag agtgctgttc agcgatcccc acgtcaaaat aatgatctgg atgtttttct
     3121 tggaatgttt tcagtccaaa ggcgcctggt atcgctgcat tgactgcggc aataggagct
     3181 gtacttgttt gcattttctt atccaaataa tccatgatga tcatattaaa tgtttctcca
     3241 gaagttactt gctttgcctc cccggtggac agatcaaatg gtacatgcca atgatatttc
     3301 attttattat cgattgccgg ctgataaccg cgaccttttt ccgtatggac gtgaagtaca
     3361 atcggatgat caatatcttt tacttcttca aataaacgga tcaccgtttc cagatcgttt
     3421 ccattatcca cgtaacgata atcaaatccc attgctttaa ataggttatt gctgctttta
     3481 ccgttggttt gacgaagctc agctaagttt ttatataaac tgccatgatt ctcatcaata
     3541 gacatctcat tatcgtttac taatacgatt agattggatt gtaaaacacc accattgttc
     3601 aatccttcaa aagctaagcc accacttagg gagccgtcac cgataaatgc taccacattt
     3661 cctgattctt tcttcagatc tctcgcttta gctaaacctg ttgctaagct gatagatgta
     3721 gatgtgtgcc ccactgtaaa atagtcatgt ttactttcat gttggttgga atatccgctc
     3781 acttcacggt aatgtccttt ttcaaaacca ttttttcgac cagttaaaag cttatgacca
     3841 taagactgat gggaaacatc ccaaacgatt ttatcctttg gtgaatcaaa tacataatga
     3901 aacgcaatcg ttaattctac tacacctaga ttcggaccaa cgtgcccacc tacatcgctg
     3961 tccttttgca agattttttg gcgcatctct tctgccaact gtttcatttc ctcaacagat
     4021 aattttttga catctgcggg gctattgatt gcatccaata catgtaatac tgtactcata
     4081 atacattctc tcctccgtac tatcttcttt taatgaaaat agatttgtta cactttatct
     4141 attctactac ttagagttga ctctaagtca atagcatttt aaaaaaattt cagagaaagc
     4201 ttgttatata agtagatctt cgcacgaatc aggactcaaa taaaaacctt ctacagacaa
     4261 ttaaaaagct ttgtagaagg tctcctaatt aaggtctcat caagaaatat gtttttttaa
     4321 taggtgaagt tcctgagcaa tatgatacaa atggatgttt gcacctttta atgagattgg
     4381 atagatcttt gtcttttcag tttctaatcc attttgatag ctagcagcat cgcacaaaac
     4441 cactcttttt ttcgcacata gacattcatc aggcaattct tctaatgaac gaaataaaat
     4501 aggatttatt tcagcgatgc cttcttccgc atacaaatga aattgggttc gataaaaaaa
     4561 ttgtttgact gaactttctg aaacaacaaa aatttctatt tcctgtctcc ccgcttgttc
     4621 actgttgatc aaagaaagac ttttgacgaa ttttcgaatg aaattttcat tccatcttag
     4681 ttggatttga tttttctttt tccactcttc caatatttct acaatttttc cgtacagaag
     4741 ttgttctttt tctgatagaa gatagatcct ttccggtaaa aatacttgtc catctgccca
     4801 caacgtacgg acaaattgta aaaaggattg cttgaaaata aaattttctg catcgtcgat
     4861 ttcagcgata ctaataatct gtaaaatcaa ttcattcagt gataggtcat tgagaacaaa
     4921 atttttataa acaacttcga aatcttttct taacagctcc atgttctcat tggtataatt
     4981 tctactgttg aacaaagaaa aaatataata gacatcttct tcttgtaagt ctagaattcc
     5041 tttttgctct tccaatcctt gacagactaa ttgaaataac ggcttcgttt ctgcgctttt
     5101 cttatcttct tctttcagtc caaggggaaa tgcctttctt ccgattgcga tctctattcc
     5161 tcgtatgata aaggactttt catcttccga atatcttctt ttaaaaaaat gagtctctac
     5221 ataatcaatc aactcgttac aagcttcttc tacatacgga tttttatttt catagccttc
     5281 ccaattgaca taacgtacta aagctagcaa gatgttccgc gagtcaaatt ctgggataat
     5341 gatttcttta tttttcaaat aatcattttc tttaaaaaaa tccaatacga tttttttgat
     5401 tgtatatact ttgctcaaag aaataaattc aagatcagcc agttctgtac tcgtgaatat
     5461 accattaaaa taataactta aaaactttaa aaaataagat tgaccgtaca gaaactgtgt
     5521 gagctcagaa agagcaatac catgtttgaa tacgagttta tacttatgct tctctcttac
     5581 ttgttctttt atacaatatt ctagacactc ggctagactt tctagatcgt gaaggatcgt
     5641 tggtgtagtc acccttagag aaatagctgt ttgttcgatg tctatttctt ggaattcaaa
     5701 taattgttca cacagataca cttttctaaa tatatttcgc tcgatgtaat tttccagcat
     5761 tcctgataca cgcctccagt ttttcttatg attttattta atcatatctg taaatcgtat
     5821 ttttgtttcc tttttattta gaaaagccct ttcaaaaacc aataaaaaaa gaaactggga
     5881 caaaagtcaa acaagctcac aatttcagcc tcattgtagg tagctttgag ccgtttggac
     5941 ttttacccca gcctctttca agaatggtta ttcaaagatg tcagcgtcta gttctttgac
     6001 tacatctttc aacaattgga taccagctgc gctgttacca aatggatcta gtgccgggct
     6061 gaagacacct atgccgtaac gatttggcac agctgccatc aacccgccgc ctacaccgct
     6121 ttttgcaggt acaccaacat ggacggaaaa ctctcctgat tcatcataca aaccagcagt
     6181 tgtcataatt gatttgacga ttgtagcact ttcttcagta atgatttgtt tgccattcca
     6241 aggagcaatt cctttatttg ccagcacagc agctaatttt gctatgcctg tagcggtgac
     6301 attgagggag cattgtctga aataagcatc gagtatctcc ggtacgtctc cttcgatcat
     6361 ttgattacct ttcatatagt aagcaagaga gcgattgata tcacctgttc ttgattcaga
     6421 atgatagatt tcttcattca atgtgatatt aggatcatca cagatctctt tcataaatga
     6481 caagattcgc tcaaattttt catctgcatc tttaccggca atcaacgagg ttgttgcaat
     6541 cgctcctgca ttaacaaaag gattcattgg atgtttgcga tgattgattt ccatattcat
     6601 gatagaattg aaggcaaaac cggttggttc cgtgttgatt gtttgaaaga ctttttctat
     6661 tccgttgtct ttgattgcta ataacaatac gggaactttt gacatgcttt cgatggcaaa
     6721 gcgcacatcg gcgtctcctc cctcaatcag ttgttttgta tccaaatcat aaatcgccat
     6781 acctaattgt ttgggatcca cttcagctaa tgctggaatg taatccgcca cttgtcctgt
     6841 ttgataatag tggaaatttt ttccaatcaa ttgttctaat tcttttactt ccatttgaat
     6901 aaccagcttt cattttttat ttttctgcat gctggaattt atgacggcat gcgtagatcg
     6961 taaatggaat aactaagacg ataagataag aaacaatcag aatcgtttca taaatcccat
     7021 gctgtccagc tgcgatacta gcaggtggaa caaatgagat acaaaaagca aaaagtgaag
     7081 tcaaaagacc taaacaagca acgattactt ttacaacttt tccgcctgga acctgatagg
     7141 ctctttcaag actgtcatct ttaaaaacca gcttcaaata gcccgcgaaa aacataaagt
     7201 acaacatcag gtagattaca acagtcaatg acatcgctgc aagaaatgaa agattattcc
     7261 ccccgccgct taatgtaaag acggctgccc agattgttgc aacgatccca tttgcgatca
     7321 tcaaatgagc aggaacaccg tgtgtgtttt cttttgcaaa atattcagga agtaatcctt
     7381 cttctgctgc tacgtgaagt gcttttgaag gagaggttac ccaagaagca atttctccga
     7441 tgacacctaa acaaacaaga atcgcaagaa ttttcgcaat ccatgcaaga tttggatcaa
     7501 tgtgtcttaa taggaattct aacgattgaa tcacccctgt attaagagac aaaccatgtt
     7561 gaggtactgt agtagcgacg gtcaatccgc caaaagtatc taaaatgatt gccagaataa
     7621 ccaatacaaa cattgctaat ggataatcct ttttagggtt ttccatttca tttgcatgac
     7681 tggcagatgt ttctacgccc atatatgcta aaataaagga aacaaaaaca accagcgtag
     7741 gcaatttcga aaaatcagga atcaatgttt tcatagacag tatcgtttgt agtggagcgc
     7801 ctgaagcgac ataatggatc cctaagccaa acaagactaa tgctggcaac aagataccag
     7861 ctacaaaacc gatccgagca atcaatgaag tgtatttggt tccttttaac tgtgagagta
     7921 aaatagccca gaaaataatc aataggattg ccaatttcag atatttattg ttttgtatag
     7981 caccaatacc cgttgcatag gataatgcac cagttaagaa atacagcatg gtcaagtagc
     8041 caactgttat ctcaaaccat tggaaaaaga ttgcggcaaa accgaatcgt tttcccaatg
     8101 tgcgactgac ccaagtatag acaccgcctt tttcccatcc tttcaccgtt gccatctctg
     8161 ctgcgcacaa tgccacaggg ataaaccata gcaaaccgcc taataacaag aaaaatacta
     8221 agctgaagcc tgaggtcgcg aatgtgggat attcatataa tgaaacgacc attgagatgg
     8281 tcatagccaa aaagccaaag aacgttaatt tttttgcagg aattttcgtt ttgctttgtt
     8341 gcatgaatct tcctcttttc tatttgaatt tgtttcaatt gagtttgttg ccaaagaaga
     8401 aaaagtccaa tccttcttct tgttcttctt ttgtagtagt gatcgttgta aagtgcatcg
     8461 ccgattttaa aatcactgtt gtcagcacat aggcatacac ggcagtaatg atgacaccaa
     8521 tcacttgaat ccctaagaag taccagctgc ttggattcgt tagtaaatgg ctgttagatg
     8581 caaagatccc tatcagaatc gtacctgtga atccgcccat gccatgaaca ccccacacat
     8641 caagtgcatc atcccattgt ttctttttac gaaaatcaac agcagcatga cagacgatac
     8701 ccgcaatgat cccaatcagc atggcacttt ttgcttctac ataaccggca caaggcgtaa
     8761 tggctgccaa tccagcaacg gatcctgtta gtacgtcgac aaaatcaaag ccatttcctt
     8821 ttacttttgc aatgatcagc caagtaatca tcgcaaatgc aagagcgatg aatgttgaac
     8881 caaatgcatt gactgcttgt tcatctgctc gaagtgcacc accggaatta aaaccgaacc
     8941 agccaaacca aagcagtcct gtaccgatag cagctgccat aagactgcta tgctgacttc
     9001 tttctcctaa aggaatcttt ctttgaccaa gtgtgaggat acatgctaaa gagccgaatc
     9061 cagctgtcgt gtggatgact gctccaccag cgaaatctct aaatcccatc tgatccaaaa
     9121 atccgccacc ccatatccag tggcaaacag ggaaataaat cagcagattc caaaaaataa
     9181 caagtataat atagcctttt aaattcaatc tccctgcaaa agctcctgtc atcaaaggaa
     9241 cggtaatcac gataaacatc agttggtaaa gaaagaacat taaaaatgga atcgttgcac
     9301 catgaaacat gttggggaag cttgtaacat gtctcatcaa aaagaaatct gccgggtttc
     9361 caataacacc gccgatatcc cgtccaaagg ccaatccgaa tccaccaaat acccacataa
     9421 tcgttgttat cccaacggag agaaaagatt gagacatgat cgttagtata ttattcttcc
     9481 gtgctaatcc gccgtaaaaa aaagctagac caggagtcat caggcaaacc attgctgtac
     9541 ataaaatcat gaatgctgta tctcctgtat tgataggtaa tgtattcatt tttttcgcct
     9601 cctttctacg attacgagta taaaagaagg tgtagttgtt tttttcttct gtttttttga
     9661 attaatactt gcatttataa aagacagaca attaataaaa gcttgatgtg ttgtgctctt
     9721 tttcgttttt cacacaaata aagacaaaaa ataaacggtc ttctttttat agaaaaccgt
     9781 ttattttcgt ctttttttat ttttaaagat attttttcat aaggaataaa
//
LOCUS       DARBDV010000100         9362 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000100 DARBDV010000000
VERSION     DARBDV010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.102"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(93..761)
                     /locus_tag="SCM56_002448"
     CDS             complement(93..761)
                     /locus_tag="SCM56_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG1111189.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            895..1182
                     /locus_tag="SCM56_002449"
     CDS             895..1182
                     /locus_tag="SCM56_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111190.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1598..3136
                     /locus_tag="SCM56_002450"
     CDS             1598..3136
                     /locus_tag="SCM56_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEG1111191.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3133..4098
                     /locus_tag="SCM56_002451"
     CDS             3133..4098
                     /locus_tag="SCM56_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1111192.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4085..5407
                     /locus_tag="SCM56_002452"
     CDS             4085..5407
                     /locus_tag="SCM56_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEG1111193.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5470..5574
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5648..6391
                     /locus_tag="SCM56_002453"
     CDS             5648..6391
                     /locus_tag="SCM56_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1111194.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6406..7398
                     /locus_tag="SCM56_002454"
     CDS             6406..7398
                     /locus_tag="SCM56_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1111195.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7410..8147
                     /locus_tag="SCM56_002455"
     CDS             7410..8147
                     /locus_tag="SCM56_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1111196.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8137..8859
                     /locus_tag="SCM56_002456"
     CDS             8137..8859
                     /locus_tag="SCM56_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEG1111197.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8943..>9362)
                     /locus_tag="SCM56_002457"
     CDS             complement(8943..>9362)
                     /locus_tag="SCM56_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEG1111198.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3069 a         1607 c         1972 g         2714 t
ORIGIN      
        1 atttttttat aaagaagatt ttactgaata atatcatttt agtttacata atgatatttc
       61 tgtaaattaa ataaagtagt gaaaaataag aatcagtgct ctaactgttc aagaggatca
      121 agtggttcaa atgtttctgt agtctgtttt gcttccaatt ggggcaaaac agatccaatt
      181 tccagatact tcactttatt caatgtataa gatagaatag aacagctaag taatgtatac
      241 agaaaatgtg tgagatccaa ataaagcaag gagacaacta aaataccaaa agtaatcaag
      301 aaaagacttt tgccaatctt aatatgaaaa tatttttcaa tggcttgata aatgatatct
      361 gttccgcctg tgcttccgcc gaatcgaaag acgagaccgc aaccaatgcc agaaagaata
      421 cctgtcatca aactattgat cagtagacta tctacagcaa aatgtatatg aaggctctcc
      481 cataaatgta aaaagaaaac tagcgcaaat gttccgacga tcgtccggag aaaagcgtgt
      541 tttccaaaaa gaaacaatga gataagcaat aaaggtaaat taaaaagcag tgaagtgaca
      601 gaaggagcga atccaaaaag attatgcaaa agcaaagcaa taccagtaac cccaccatct
      661 gccagcttag atggaatatt gattcctgtc acagctaatc cgtagattcc tgcgcctaaa
      721 atgattccgc acaattctgt aaataaattt aattttttca tttctagcct cttttctttt
      781 gaaaattttc agcaacttct ttattatact gacttatact cttacttcaa gaggaaaatg
      841 aaaaaaaatt atttttggct gtttcttttt atttatcaaa taaggagtgg aaaaatgcga
      901 gcagcagact tattgacaat tcttacaaaa tcacccaaaa aaacagagta tcaaggagtt
      961 tttttggctc aagaacaaaa actggtgcct gtgacaatgg tccagataaa taaagcaaat
     1021 caatttatct ttttatttga acacggcaaa gcaccacttg ctatgaaaga catctttctt
     1081 tttttgatga agaaccgtga gaaagctgtt tattatcagt caaaagaaga actcattcct
     1141 ctttatggag tgaaagagat agaaaataaa gtggtgatat aagtaaaaat aagaaaacaa
     1201 gacgtttatg atgtcaaaac aaccattttt tacatttttg gcaaaaaagt gtcatgatac
     1261 agtataatat tcgttgaaca ggccaataaa ggtgttctcc cccacgagat ttctacttta
     1321 ttggctgttt ctttttatta aaataaaagt tgtgtgtgta aaaaagatat agctgattcc
     1381 aagtgacaat ttcgtataga tttttatggt attcgtagtg aaaaaacgag aaccaaaaag
     1441 ttgatccgac tttgttgctt ggattctttt tttgtcagca tatttagagg tatttttttc
     1501 ttttttttgt tcgtgctgta aggttaaggc aagacaagtt agttgggaaa aaggaaagaa
     1561 atgtttatac ttaaagaata acagaaagga aggtcgcatg aataatttta tacaaacatt
     1621 aagtgaaaga aaaggcgagt tgctggaagc tgcatttcaa catctgtcca tttctctcat
     1681 ttctttgctg attgcagcac tgattgctat tcctttagcg atttgggcag ccaatcataa
     1741 aaaaatggca gaagtcttat tgcagatcac gggtgtgctc caaacgattc cttcacttgc
     1801 cttattaggt ttacttatcc catttgtagg gatcggaact gttccagcat tgattgcact
     1861 tgttatttac gctttgcttc cgattttcca aaacacgtat attggtctag ctgaaattga
     1921 cccgtctatt gaagaagcag cggtagcatt tggtatgtcc agaatgagac gcttgctgaa
     1981 agtagagctt cccatcgcat tacctgtcat tatctcaggt atccgaactg ccttagtttt
     2041 aatcatcggt acggctactt tggcagcatt gatcggagca ggtggattag ggacatttat
     2101 tttgttaggt attgaccgaa atacacctgt tttgacattg attggtgcga tcagttctgc
     2161 cttgcttgca attatattca gcggactgat tcgctggttg cagcataaga aacctcgata
     2221 ttccatcatt actttagcag cagtggtgtg tttatttggc ggcgtttctt tatatcagtc
     2281 tgccttgttt aaaaatgaaa ccatcacgat tgctggaaaa ttaggagcgg aacctgaaat
     2341 cctgatccaa atgtataaag aattgattga agacaacaca aaaacagaag ttgaattaaa
     2401 accgaatttt ggcaagacga gttttttgtt caatgcactg gatcaaggtc aaatcgatat
     2461 ctatcctgag ttcacaggta ctgtcctaga aagcttggtg aaggtacctg aagcaatgaa
     2521 aagtgctgat ttgacaaaag aaaaaacata tgacgaagca gaagaattat tgaataagca
     2581 attcgatatg acattattaa aaccaatggc ttaccaaaat acgtatgcct tggcagtcaa
     2641 gagatctttt gcgaacgaac atcaacttaa aaagatttcc gatttggcag ctatctctga
     2701 tcagatcaaa gcgggattca cactggaatt tattgatcgt tcagatggat ataaaggaat
     2761 ccaagaagca tatggcttga atttttcaag tgtacaaagc atggagccaa gtttgcgtta
     2821 ccaagcaatc aataatggag atgtcaatat tgtggatgcc tattccacag atagccagct
     2881 caaggagtac gatctagtga ctctagaaga tgatcgaggg ttattcccag cttatcaagg
     2941 cgcaccgctg atgaaaaaag attttgctaa agaacatcct gaaatcgtta aagcactgaa
     3001 taaattaagc ggaaaaataa cggaggatca aatgattgat atgaattacc aagtcaatgt
     3061 cgaaaagaaa aagccgacag aagtagcacg aatcttcctg aaaaatgaag gattagtaaa
     3121 ggaggaagat cagtgactaa attaatcgag ttcaaacatg tgcaaaaaaa gtatgatgga
     3181 aagtatgtca tcgatgacct aaatctggca atcaaaaaag gagaaatctt tgttttggtt
     3241 ggtccttccg gtagtggaaa aacaactact ttgaaaatga tcaatggatt atctaaacca
     3301 tcagcaggag acatctactt caaaggaaaa tcgttaaatg aatataatct gcagaaaatg
     3361 cggtggaata tgggatatgt gctgcagcaa atcgctttgt tcccgaccat gacagtcaag
     3421 caaaatattg aagtcatccc tgaaatgtta ggttgggaaa agcagaaaag agcagaccgt
     3481 gtagatgaac ttcttcaaaa agttggctta tctcctgata tttatcgaga tcgtatgcct
     3541 agagagttgt ctggaggaga acagcaacgg atcgggatca tccgagctat tgctgcaagc
     3601 ccagatgtga ttttgatgga cgagccattt agcgcattag atccgatttc aagaaactca
     3661 ttacaagaat tggttttgtc cttacacgaa gaattgggca cgaccatcgt tttcgttacg
     3721 cacaatatgg aagaagcaat caagctaggt gatcgtattg catttatgaa ggatggagaa
     3781 attatccaat gtgacacacc agagcagtta ttgatgaatc ctaaaaacga ctacgttcgg
     3841 catttctttg atgaaccgaa acagacgaaa gagtggcgag tagaagattt agtcgtcaat
     3901 ggatatttct taaatgaaat accagaaaat gctcgtcaac aagtttgctt tgatacgcct
     3961 atgaaagaag tatattcgct tctttcggtg tatccaagca ttgtgatagt agaaaagcaa
     4021 agaagtattg ggtcgctgac aagtaaagaa atctttgctt ttttaagtcg agaggaggaa
     4081 ggaaatgaaa acatatgatg caatcatcat cggttcaggt gtgagcggct taagtgctgc
     4141 ttatggattg aaagaagcag gtaaaacagt actagtggtc gaagaagatc tatggggcgg
     4201 gacttgtcct aaccgtggat gtgatcctaa aaaagtcttg ttaagtgcag tagaggcacg
     4261 taatcgggta aaacaattga gtggaaaagg ttttaacgag atcccaactg ccaactggga
     4321 agaacttcaa aaattcaaac gaacatttac ggatccagtc ccagaaagcc gaaaaaaaca
     4381 attagcagaa gctgaaatcg atcatctttc aggaaccgct cgttttttgg atgattcatc
     4441 tattgaagtc aacgaagaag tcttccatgc ggattactta gttttggcaa ccgggcaaag
     4501 acctacgatc ttaccagtgg agggaaagga atacttgaaa actagtgcag actttttatc
     4561 attgcctgta ctgcctaaag aaatcatttt tatcggtggt ggttatattg cttttgagct
     4621 agctacgatt gccaatgctg cagggagcaa agtaaccatt gtccatcata atcaacggcc
     4681 attaaaagaa tttgaggcgt ctttggtaga ggaagccgtc catcaaatgg aagcatctgg
     4741 cattcagttt gcttttggtg ttgaaaccca aaagatcatt tcagaaggta ctcgctatcg
     4801 tttagtcgga aaagagacgg aattagtagc agatatgatt ttttgtgcga ctgggagaca
     4861 gccgaataca gaatctttag cacttgagca agcgaatatc gtctttgata aacacggaat
     4921 tgcagtcaat gattatctgc aaacgagcaa tccaaaaatt tttgcgtgtg gagatattgt
     4981 ttcaaggaaa accccaaaat tgacaccagt cgccactttt gaaggaaatt atgtagctaa
     5041 gcggataacg gatgcaacaa gcgaacctat caaatatccg attataccta cgatcgtcta
     5101 cgccagtcct aaacttgctg aagtaggagt tacaaaaagt cacgcctctt cgtcagatca
     5161 agtggtagag atggatctta ccagttggtt cacctatcat cgtgtcaatg aaccagttgc
     5221 taaggctgaa ttgacctttg accagcaaaa ttatttgatt ggtgctgcag tgattagcga
     5281 gcaagcagac gaattgatcg atgatttgac actcgtcatc aatcaaaaat tgactaaaaa
     5341 agagttggat agctatatca tggggtatcc gacattggca agtgatctct cctatttatt
     5401 gaaataatca gcatgataac taaagcgctt gttgcttttt ccttcttgtg ctacacttta
     5461 aaaaatgaat acctgtttct ggggatgtcg tagacgacat gagagtaagt tgtgaactta
     5521 gacccattga acctgtcagt taatactggt gaagggagaa acatagcaga cgcttgaagc
     5581 gggttactat gtcgtgccca tttcaagcgt tttttgttat tcatattaga agaaaggagg
     5641 taacaaaatg aagaaaaatc tttatccagc agtcagtatg gcattcattt taattatttg
     5701 gcagattgtt gtcatagttt ggaaaatacc tgcctttatt ttacccggac ctgctgctgt
     5761 attttctgca ttagcggcag atgcacaagt attactttcc cattctgtca cgacattaaa
     5821 tgaagcagtg cttggtttgg taatagctag cgttctttct tttattactg ccttggcaat
     5881 ggattattgg aagtttttag aaatgtcttt ttatccgtta ctagtgattt cacaaatgtt
     5941 gccaatcatg gtgttagggc cattacttac cctatggttt ggtttcggta tgcttccaaa
     6001 agtgatcctt gttgtcttga taagctattt tccgatcgta gtttcttttt cggatacatt
     6061 gaggaaagta tcaaaagagc aaatcatctt tttgaaaacg atgggggcag atacagggaa
     6121 gatttatcga atatataaaa taccagcggg tttatccggc ttcttttccg gtttaaaagt
     6181 ggctgctact tattgtgtca gcggagcagt cgttggagaa tggctaagcg ctcaagcagg
     6241 cttagggtat tacatgatac gcgtaaaaaa cagctaccag atcgataaag tttttgctgc
     6301 gatcatttgt gtcattctat tgagtttatt gctgaatggc gcttgttcat tattgaaaaa
     6361 agggtattat cgtatgcttt atatttttta agagaagggg atcaaatgaa aaaattatgg
     6421 cttttattac cgctactgtt gcttttatcc gcttgtggca ctgcaaaaga aacaagtagc
     6481 aaacaagaaa taaaggattt gaaaaaagtg acacttgttt tggattatgt accgaatact
     6541 aatcatactg gcatctacct agcaaaagaa aaaggctatt ataaagaggc tggtttaaat
     6601 gtccaaatca tcgaaccagg agataattcg acaagtattg gtctagtagg tgcggacaaa
     6661 gcacaatttg gtgtaagtta tcaagaagat gtcacttatg ctcacgcaga tggacagaat
     6721 ataccagtca aagcaattgc tacagtgatc aaacataata cttctggatt cgccacccta
     6781 tcagacagta acatccattc accaaaagat tttgaaggaa aaacatatgc aggttggcaa
     6841 agtccaagtg aagaagctgt cttaaaagct gtgatggaaa aagacggcgg ggacttttca
     6901 aaactgacaa tggtaggaag caatggagaa gggccagaaa gcttaggaaa atcctcagat
     6961 atccaatggt actttgaagg ttgggatatg atcaaagcaa aagaagcggg tattgaacta
     7021 aactatatcc cattgaaaga attagatgaa cggttagatt actatactcc ggtaattatt
     7081 acgaatgatc aactgatcaa aagtgatcca gaattggtac agtcttttat ggatgccacg
     7141 aaaaaaggct atcaggaagc tattaaggat ccaaatgaca gtgctaaact gttgcaaaaa
     7201 tatgcaaaag aaaatgatcg gacgttttta gaagaatcac aagcattctt gtcaaaaaat
     7261 tatacggatg atcctgagaa ttggggattg atggaagaaa aagtttggcg caattataca
     7321 gcttttatgc aggaaaacgg attgatcaag caagatgtac cttcgcaaga actattcact
     7381 aatcagttta taaaatagga gtgatagata tgtcaatcca aatcagccac ttgaatgtag
     7441 tcattgaaaa cctgaaaata ttagaagaca tccatttcac tatccctaaa gataccttta
     7501 catcgattgt cgcgccaagc ggagccggta agtcaacatt gctgaaaact ttaacaggag
     7561 tccagcctat gacttctgga acgatcaaag tagacgatca aaaagtaact ggtctccata
     7621 cagcatttag ttatatgccg caagaagata tgttacttcc ttggctgagc gtctatcaaa
     7681 acgttacgtt atatcaaaaa atcaatcatc taacaatcaa tgaagaacaa gtaagggatt
     7741 atctggatat ctttgggtta aacggctatg aacatttttt gccggaacaa ttatctggcg
     7801 ggatgaagca gcgaacggcc ttgttacgaa caatcatgaa tccttcttct tacttgcttc
     7861 ttgatgaacc atttggagcg ttagatgcta tgaccagagg gcagatgcaa gactggatgt
     7921 tgaagctgcc taaaaaagca aagcggacta ctttacttgt gacgcatgat atcgaagaag
     7981 caatttattt atcagacagg attcttgtgc tttccgcccg tccggcacat gtgattgctg
     8041 agattcaagt gcctgaaaaa cgacggtcac gtgaatggct actgcagcaa tcagagttga
     8101 agcaaacgat ttatcagtta ttggcaggtg aattagatgt taagtgatgc tcattgtcat
     8161 ttagatggga gtcagagttt agcactcctt cagcaggaac acacgatttt gacgatcatc
     8221 aattgtgatt cgccagaaga atggaaggaa aacagacagc ttgcagcaag taagactcaa
     8281 gccttaagtt acggtatcca tccttggaaa gcagatagct atacgtttga acaagttgag
     8341 ccttttttga aaaaagcaag gatcatcggt gaaatagggt tagataatat ttggacgaat
     8401 gtaccgatga ctacacaaaa aaaagtattt gagcgacagc ttgcttttgc agccataaat
     8461 gagaaacctg ttgtactgca cacaaaagga tgcgaaaaag agatcttaaa ttatatccaa
     8521 gcttacccta atcactattt gatccattgg tattcttctt tggactatca acaagactat
     8581 attgatttag gctgttattt cagcattggt cttgatttga aaaagaatcc agcagtctgg
     8641 caactagcta aagcagtccc aatcgaccac ttacttattg aaacggatgg actacatgca
     8701 gcagagtggg ctttaggggg gattttttca gaaaaagatt atgtctcact gcttgaagaa
     8761 cagttaaaga caatcgctga gatcaaagag ctatcagttg aagaagttaa aaaaaatact
     8821 tatcaaaact tggaacgttt cattagatta ggtaaataaa aaagagatgt gacacaacta
     8881 gtgtcgcacc tctttttggc tttctttgta taaaagtaaa ctgtttgatt aataaatgaa
     8941 tcttagtacc aaccgtttgc ttcccagaat gctttcgccg cttcccaaga accataacgt
     9001 gaagtaacat attgttctgc tactctttct tggtttgcag cagagtagtc accatttaag
     9061 taagaagaat ctaattggta acgtccgatg taacgtccgt tcgttgctgt gtaagaaccg
     9121 cttgattctt tttgtgcgat ccattctttt gcagagcttg tgcttgctgg agtagcagca
     9181 gtttctgtta cagcaggagc aggttgtgtt tcagcaacag gcgctgcttg tttttccgtt
     9241 tgaaccgttt cttgtacaac aggttgttca gcttggactg gtgcttcttg aacggctgct
     9301 tgaggttcag cagctgcttg tgcagagcct tctgttggga ttgttaattg ttgtcctgag
     9361 ta
//
LOCUS       DARBDV010000101         9358 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000101 DARBDV010000000
VERSION     DARBDV010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.103"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="SCM56_002458"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="SCM56_002458"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEG1111199.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="SCM56_002459"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="SCM56_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEG1111200.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="SCM56_002460"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="SCM56_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEG1111201.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="SCM56_002461"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="SCM56_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEG1111202.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="SCM56_002462"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="SCM56_002462"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEG1111203.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="SCM56_002463"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="SCM56_002463"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEG1111204.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="SCM56_002464"
     CDS             complement(8053..8523)
                     /locus_tag="SCM56_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HEG1111205.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="SCM56_002465"
     CDS             complement(8569..8994)
                     /locus_tag="SCM56_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEG1111206.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="SCM56_002466"
     CDS             complement(9061..9330)
                     /locus_tag="SCM56_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEG1111207.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
BASE COUNT         2355 a         2095 c         1621 g         3287 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc ccctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatggaat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta attttttt
//
LOCUS       DARBDV010000102         9303 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000102 DARBDV010000000
VERSION     DARBDV010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.104"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..338)
                     /locus_tag="SCM56_002467"
     CDS             complement(<1..338)
                     /locus_tag="SCM56_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEG1111208.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLV"
     gene            complement(328..2502)
                     /locus_tag="SCM56_002468"
     CDS             complement(328..2502)
                     /locus_tag="SCM56_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HEG1111209.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(2518..4860)
                     /locus_tag="SCM56_002469"
     CDS             complement(2518..4860)
                     /locus_tag="SCM56_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111210.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(4882..7425)
                     /locus_tag="SCM56_002470"
     CDS             complement(4882..7425)
                     /locus_tag="SCM56_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG1111211.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(7435..7833)
                     /locus_tag="SCM56_002471"
     CDS             complement(7435..7833)
                     /locus_tag="SCM56_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1111212.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(7914..8198)
                     /locus_tag="SCM56_002472"
     CDS             complement(7914..8198)
                     /locus_tag="SCM56_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111213.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(8222..9220)
                     /locus_tag="SCM56_002473"
     CDS             complement(8222..9220)
                     /locus_tag="SCM56_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1111214.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
BASE COUNT         2646 a         1996 c         1458 g         3203 t
ORIGIN      
        1 acgagaaaac ttgggacact tttaacatgg taacgttgta aaaaagtttg aagttcttct
       61 tgtcttggtt cttttcttgt ttcaattcga tatacattcc tagagaattt tttgtcgtat
      121 ctttccaaga aagattcaac ttcttggcat tttttacaat catttcttgc tagatacaca
      181 aaaaatgatt gattcgtttt ttgaagttct agtacttctt ctaaattact attgattagt
      241 tttccatttg attcttcgtt agtttttatc agtttaaaac aaccaaataa gaggaaacta
      301 acaataagta tcctaacaat aactttctta tttcgcatat tgtttctcca aatttggttt
      361 ccgaatatag acgactcgcg gtggttcctg gacgtcttca gctgttcgat acaccacaaa
      421 ttcgcctgtt ctcaatgctc gaatgcgatt cggattgaat ttaaagcggt ctacgtctcg
      481 tttcgtgcct ctttctcctg tccaatcaac ctttcccata tcagaataac ctgcttcttg
      541 tgtcgtcatt tccgttaatt caatgtcttc ttctgtcccc attaaatctg cccaataatc
      601 aatttctgtc gggtcatttg tcttcccaat aaagaacgta ttcacattcc caatcaactg
      661 ttttcgaagg acaggatcaa ttctatcaat gtccgcaagc gtttgagggg catacgccgc
      721 atgcacccca gcgcctcgtc ccttattgac taagtctata aggttatcat ttagataaga
      781 agctggttcg tcaaatatta ctaggacttt cgagcgattg gctgtttctc ctgcttgttt
      841 atacacttct gacgcaaaga aattaatatc tcccacaagc atttgagcca atgtccgaat
      901 aaactcacta taaatcaatc cgtttaacga gatataaaca atctttcctt ctttaacaac
      961 ttcttgaata tctaattctc tttttcctcc agaagtatca aagagttcac caagctcact
     1021 atccaataac atattgatat tggtctgtaa attctcagaa gtcgcataca aataaaacgg
     1081 gttcttctta tgttcgtaac gaacaaacaa ccgtttaaac aacgtttgct cgatttctgt
     1141 catgagagaa atgttttctt ttaataatag ataatacgtt tctaagtcaa cggttttacg
     1201 atcatgttca acgattcgct cttcacttaa ctgttccaca tcggcaaaat cacgttctgt
     1261 tttgtccatc gcttcattct ttttcgtttt tttcttcgtg gttttcttct tttctacgac
     1321 cactttttct ttaaataaat ctggattttt caactgaata cgatccgcaa acaaatgtaa
     1381 gacgtttcgt ggcaataaat aatgttgtaa ccaaactaga tttctttcaa ttttatggct
     1441 cttttggaac tcgtctaaaa gctgaatcgt tacctgtaat aacgctttgg ctgctgtcga
     1501 ataatacacg ctttctgttt ctgctagatt ggttaattta tctcgaatgg caatatcatt
     1561 gccatttttt acaggattat aacgcaaatc gccaccgtcc acaaagactt caatcgggcg
     1621 attgtatgcc tctgccactt cttttaacgc ttgtaaagtc tctcctgctc ctttcccatc
     1681 gactaagata acggggatac gaaacttaca cgcatgttct gctagtaatt ggatcaccgt
     1741 cgtttttcct gaacctgtag tcgctggaat aatcacatgt tggttcaatt cactaaagtt
     1801 catcacataa gggagcccat actcatctgt tcctaataga atcactttgg ctaatgaagc
     1861 cagttgttta ttccgcaaag caacgtcttt tatgacaatt gctacctggt acgcttgctg
     1921 atactctgct gtatattttt gggactcctg aaatttattc ttccgacgtt ccttgtattc
     1981 tttactattt agcaaagctg tttgccgttc tttctttgaa acaacttttc ttttttcaat
     2041 aaaagacgca tacgcaatca aacaacttcc tgcacctgtc ccacctaaaa ggagggcaaa
     2101 aatcgtttgt ccattaaact gcaacgcatg agaaaacaag tttaatggaa ataataagta
     2161 acacgacaaa taccctctag gaagaacaaa aataaaacaa aatagattta atagcgtaaa
     2221 acctaacgca aaaataaaaa aaagagagct tcttcgtttt aagaagctct catactcaaa
     2281 gtcataaata tgtgcacgtg tctctttttt atctatcata aagacaatcg ctaaaggaat
     2341 actgcctaca ataaaaaatg gatatagaca aacacctaat aaagtgacga taaaataaag
     2401 ttgttgaaac cgactttttg gcgtttcttt ttcttgtttt cttttgcgat cgacagaaaa
     2461 catttgctca aaatgtgtcg gtcgttttgg tgcattcatc atactatcga tttcctccta
     2521 tcgttttcgt tgtttcattt ctcttcgata aatcggttgt ttttctcgtt ttcgtttcat
     2581 ttttgcttga gttgctcgaa actcactccg tgtcttcggt gcttgttttg gtgattttgt
     2641 ttgtttcatg gttgagtatg ctttagatac ggttgaaggc gctttggttt gtgctgttac
     2701 tcgattgact gctgacatat gttcccgaac cccctctgga cgtcgaacag atttcggtgt
     2761 ggtagaacct gttgctcgtg gtacattccc ttttcttagt ggtttttgta ccgcatgttt
     2821 ttgtcctgca actgtccggg ttttcatcga acttgttttt gttacgggag taagtgcctc
     2881 cttccgacga cgacttgttt gtccctgacg acgttttcga tcgttcaatt ctgttctagg
     2941 cgtatgcttc tggccataag acttgtgttt ttgctttgga ttcgctaatg acaccaccat
     3001 tgcagaaccc tgattgcgat acggactacg agacgcacct tttctacttc tttctcttag
     3061 actaggattg ccttgcaatt ctcgttgtaa taaatcggct gtttttcttg cctgtttctc
     3121 cattgctgtt cgttgcatac gttcttgact ttgctcttct gcttgtttcg cttgatcatg
     3181 caagtctctt gctttttgtg ccatacgttc catacgattt ggtgctttat gacgaggttg
     3241 ttgtgcggat tcacgccgaa aagcacttcg tgtggcttgt ttcgccgttt gtggcatacg
     3301 aacgtctgag ccacccccac gttttcgttg acgttcttgt ctcttccgtg cgttttcttt
     3361 ttctttttgt ttctgtgctt ttctcgcttc tttgcgttct ttcaaccgtt gtttggcttc
     3421 ccttctcaat tttgcttctg ccccaaatgg atttcccgca aaagctaacg ctcctcgaac
     3481 acccattctt ccagtaaata gactgcctaa aaagcctcgg aacaaataaa ttcccaacgg
     3541 cgcaaacaaa taaacaatga tcaagagaat tttttcccat ggatcgtctt gtgtactact
     3601 taattggaac ccgtaactaa gaaaagaaga aaaaaggata catgcaaaac ctgtcattcc
     3661 ttttaaaaac gtcaaaatac caaaaacttt ggcatagtta ccaaacacat tcattgtgac
     3721 aagaaacaat cctaaaaaaa gtaagaacgg caacatcggt actaacaata gttgcatagt
     3781 tccaataatc aatcgaaaca tacttaagac aaaataaaca acggtttgaa tcagattcac
     3841 gacaaaataa aacaaggcaa atgctgtatt tttccaacta ttgtaatatt gtatgttctt
     3901 gtttttcagt gtatctgctt catactctgt gactttgtca tgaattttct cccctatttt
     3961 tgtacctaaa tcattgtcca ataaaagatt gattcgatca tattcttttc cagcatattt
     4021 gactttcttc tcacgaattg tgtctacatt ggttgtgcca taatttaaca acagatatgg
     4081 ttcataaaca ttactataaa agacccgagc cgccatcaat ttgcctgccg tttccacttt
     4141 tttatccgct gttacctctt ctgtttcttt tgtttcataa tttgtttctt cttcaggtgt
     4201 taacactgga ttgaccttca cgaacgccgt ttccactgta gaatctaaag aaaaaagaac
     4261 atcaaaaaga ttcttatttg tcccagaatc ttttaatacg cttaacccag taaaaataag
     4321 gatcgtcata agaaagacac ggagtgcttg tttcattcga gcctctccag catatttgat
     4381 tgccatcata attgccgaaa aactgattcc aatagttcct gcaatactta acatattgtc
     4441 cgcaattttt tgtgttaagt tttgaatggg ttctcgaatg gcggaaataa tatctaattc
     4501 aaagagaaat tcaaccattg aagcattgaa ttctcctaac atttttacgc cactccaaac
     4561 aaaatttttc atcactcctg caacattgta cgatagcttt tttttaaggt tcattgactc
     4621 atagtcattg gtcatcaatt caaacgaatt ctttttgtaa tttttataca attgatccac
     4681 atcagtcgtt acttctttta cttctacttt atcgttggca ttctctcctt cagaacgatc
     4741 ggtttcagtg acacccgcac catcattcat ttgatcggca gaaacagtag ccccttgtag
     4801 acagaaaaaa agacccccta ataaaaggag cgtccaccat ttcagagatt tttttcgcaa
     4861 aaaaaccacc tcttttttgt tttatcgttg tttttcattt tctaacattg cttgaatttt
     4921 ttcatcttga ttttcagtag aagaaacagc caataaccaa ctatcaaaca acacatcaat
     4981 ggcaatcggt tgatttcttc ccaaatgatc ctggaataaa caggttcctg tttgtaattg
     5041 ttccatcatt tctaaattgg ctgaattcac ttccatatca aagaaggaca atagtttctc
     5101 tcgtgcttct ttttgtttcg gacgaaacgc aaatttgtaa gaaattaact cttttttatc
     5161 ttcttgatcg taatctttaa acgcttgcac aacaaggtaa atatccgtat tattcgcacg
     5221 tccttttcgc aagctatcat caatcaaata gcttccttgt gcggtatctt catacccttt
     5281 tgcctcgtca taaataatcg cagcgtcttc ttctttgttt gtagaaaaga tatacgtaaa
     5341 cttcataatg acttccataa ttgccattcc tgcaagttgt tcgctattta atcgacccga
     5401 atcaatctct ttttgtgtcg gaatgcgtat tccttgtgtc cctaacacgt tgatttgtga
     5461 atcaaaacgt atcggcgtac tgtgctcatt cccaatgaga atctttccta aaccaacgct
     5521 atgccctaaa atcaatttag ctagttcttt gtcacttttt cgaatttctt cgataacttg
     5581 acttaacgaa tgcttacctg gcatggtgta aactacatcg atggcgtctt ggatcaccgt
     5641 tttcttgtgg ctcgctgttt ctcgattggt atctacttct cctaacattc ctaacacatt
     5701 tttagcggtt tgaattgcct cttcttttgg taaaaacaat agaggatcaa gcatccctcg
     5761 acagctttca tctgaagata acgaaataaa attaattcgt tcatacatag ccacaaactc
     5821 tggtatgtgt ccgaattttt caagtgcctt taaaaagaac ttttgggttt catttttcgg
     5881 atcaatataa agaatttttt gacctaaaaa catcagccat aaaaaaatat attttacaag
     5941 gaccgatttt cctttacctg gtggaccaat aatcaacgta tttccattgg tatgttccgc
     6001 gccttcaata cttttctttg ttaaattggg ataataccat actagtttac tagatagcgc
     6061 taacgcttct ttcgcagatt taaacttttt tgcaataaca atccgtccta aaggaaatcc
     6121 tttcttattt cctacttctt tctttaagtc caagccataa tctgccacaa aacctgtcgt
     6181 agcgacctgc tcgtaactac gaaaacgttg cgtacttcca attaagcatt gattaaacaa
     6241 cgttagctga tccgctaccg atcgataaaa tttgaaatct gtagactcaa tcgcaaattc
     6301 aatatctttg actcgttctt ccaattcttc ttttgaagaa gcagatacaa ccagcgtgac
     6361 ggtagaataa cacaatcgtt tttctttgcg cttcagctta tcttttacat ccattaaccg
     6421 ttctgtacca aaatttaata catcgtctga atctaaaatg ccgtcaacgg tctcatattc
     6481 tttttcttgt tcataaatcc gtccagccat tttattaatt tttcgttcat cagattttga
     6541 atgctcaaat cgaagtcgaa tatgcgtatc aattggaaaa gataagctat cctgaagatt
     6601 ttgaataaag ctactcccat acatggctaa aggagcgtcc gtttgactta aaaacgtcga
     6661 ataatgcgtt tgattcaact gttcaatcgc aatgtatcct gcttcattcg aaatgacgcc
     6721 ctcgcaaatt tccattggcg taagctgtct ttcaggcaat ttttcttctg atcgatggaa
     6781 aaaataataa taaatttgtc caatctcttg ggcagataag cgctctatcg gcttataatt
     6841 ggacaattcc gtatagagct ccttttctaa tgtttcatac gttggtaaca cactacttat
     6901 aggaactcgt tgtcccgtaa tacgatagat aaacttttta aaaacatgct taagcaattg
     6961 aaaatattcg atcggatcag acactttcat ttctgtagtg aaacgcacaa acagatacgt
     7021 tcgatactcg tttaattgca cttcgtcttt taaaatcttt cctgcctgtt caaagtacac
     7081 tttcccaaga tctgcgcatt ctccctttac caaatctcga atggtttctt caatgtgcgt
     7141 atctaggtta aattgacgtg gaatattgat aaaatgatag tcatattcat catgtccaag
     7201 tatgccttcg ccatcttgaa gatagttctt gaaaaaatca atgtcattta aaggaaatac
     7261 ttgggaagaa agttgatagc ctacccacac atcatcattt ttggtcaaaa caaaatttcg
     7321 gataatggcg tctatcggaa attctaatcg aggaagcttc gtttttttct ttttgaactg
     7381 ccaaatcccg ataataaaga cgaccaaaag aaacaaaaac gccatttact cgactcactc
     7441 cttccatttg tgtagggaat acgtcacttt ttcatggaaa aacggcacat acatttcatg
     7501 ttcaaagcgt tgtttccgtt tctgataata ggacgttcgc ccaattaaat actcgaaaaa
     7561 gtgtttattg tcccatttca ttgaaaacaa tgtccaaacg agaacaccaa tcccaaaaat
     7621 cataatcaac caggactttc gaaacagtgt ctgcaagaaa ctaatttgat tcacatacgc
     7681 aatcaaaaat aggatcaaaa gaatgaagag aaatacccca cctatgacta attgttccat
     7741 gcgaatgcca ttagaaatcc agggaatttt ttgtccttta atcccgtaaa ttttgtgagg
     7801 gtgcttaaac tcctttgtat agttgtattc catgttcact ctacctcctt ttacaaaaaa
     7861 agaagcgacc agaagttcta atcttctgat cgcttttgca gtttgtcttt ttcttatgat
     7921 aattgtatcc agggcacaat catttgcgtt acccccacgt tctcttcact atccttaata
     7981 aagataatgt tctttagcca ttcaccaata tcaacggcat tcgataacaa taagacgagc
     8041 attatcaaaa taaagacgcc aataatggca aacgctgccc gcgcaaagcc ttgggtcgca
     8101 taagcaccaa ccattagaac aatcgcaacg attgccaata gccaaatgac ttgtccagat
     8161 acatcatcaa ataacgattt aagatcaaaa agtttcattt ccattctcct ctaagttcat
     8221 tttattcatt taacgtagta acaaaataac tatcgccact tgtttttact tccattgaaa
     8281 agtttgattg gaactcgttt cctttctcaa aatagaaacg atacttccct tgcaaaaagt
     8341 agtttccttc tttatttttt actgcatgat ccagttgtac attgatcaat tcgccgcctt
     8401 ctaacccttt ttcgttagaa atcaatgcca atttttcatc atttttgaca tataactcaa
     8461 aaaaccgttt cacaaatgct tccagcttga tttcttcgct ttcagtgacc gcttgcccag
     8521 aagaataaaa tcgattttcc tgatagttca cagaattggt attcttactc tcagaaagat
     8581 tcaaaaattg aacgccatca agtactttta aaacattgtt ttgataactg actggaaaag
     8641 aaagttgact gcttttccaa tgttctcctt ctgtaaaagt aacctcataa aataggcgat
     8701 acattgcttg tgtcaaatcg atttcttcta gtttaataaa gcgaacatct ttcgctactc
     8761 gattggtagc cgtcgttaca aagcctagat cagacgcttc caatccttgt gctaaataat
     8821 gattgacgga agcctcccat tcgtttcgtt tttctggcga ataagtaaat aacctttcaa
     8881 taaaatgttg tccctcgtac ttcaattgtt ccgtagaaga caaggtttgg ttgacttcag
     8941 ttaacaatgt ttctggattg acttgttttt tcactgctaa acgcgttaat gtatccactc
     9001 gtccaaagtt taggaggagc acgacaccca ttaacagaaa caaacaaaaa aaagcgcctg
     9061 aaaatacact catggcactt ttttgcgaaa attggatcat tcgatctgac ggttctgttt
     9121 tctttttttg gaacaaaaaa cgtttctttt gtttcttttc cttctgttgt ttcggtggct
     9181 tagtttggaa cgcttgtgga ttaaattttg cattactcat gagaaatgcc ataccgttct
     9241 aacaataatt tcggtatttt gtccacaatc actacttcgt ggacaaccac acttgccccc
     9301 tcc
//
LOCUS       DARBDV010000103         9281 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000103 DARBDV010000000
VERSION     DARBDV010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.105"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..435
                     /locus_tag="SCM56_002474"
     CDS             <1..435
                     /locus_tag="SCM56_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEG1111215.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="SCM56_002475"
     tRNA            complement(759..842)
                     /locus_tag="SCM56_002475"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="SCM56_002476"
     CDS             1370..1870
                     /locus_tag="SCM56_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1111216.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="SCM56_002477"
     CDS             complement(2060..2404)
                     /locus_tag="SCM56_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEG1111217.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2558..3478)
                     /locus_tag="SCM56_002478"
     CDS             complement(2558..3478)
                     /locus_tag="SCM56_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG1111218.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3665..4156
                     /locus_tag="SCM56_002479"
     CDS             3665..4156
                     /locus_tag="SCM56_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEG1111219.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(4180..6555)
                     /locus_tag="SCM56_002480"
     CDS             complement(4180..6555)
                     /locus_tag="SCM56_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEG1111220.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6881..7507
                     /locus_tag="SCM56_002481"
     CDS             6881..7507
                     /locus_tag="SCM56_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEG1111221.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7530..8234
                     /locus_tag="SCM56_002482"
     CDS             7530..8234
                     /locus_tag="SCM56_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEG1111222.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            8426..9259
                     /locus_tag="SCM56_002483"
     CDS             8426..9259
                     /locus_tag="SCM56_002483"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEG1111223.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         2828 a         1791 c         1651 g         3011 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agattctcgt ttaaatttat aaaaatgttc ttattagatg
     2521 taatagatta aatttattct aaaataaacc tgattcctca ttttttttgt gaacgatttt
     2581 ttatttctgg caatacgcca gttgttcgat acattttttg ttcgttttca tttgcaaacc
     2641 ggccaaatac tgaatgagtt ggtaaaatat tgataaatgc atcgtcgtcg atttcatgaa
     2701 cgatctgatc aagatcatac atttcgtagc gggtgatgac catcatgatc atgttccctt
     2761 gtttttttga atagccaccg acagacggca gtaatgtcat tccacgtacc atccgttttg
     2821 ctagttcttc tgttacctct tctggcttga cggtaacgat cattgctgtt actttttgat
     2881 gactcgtgtg gatggcatca attacttgag tcattgcata aatggaaata atcgtataca
     2941 aggcactttc ccagttaaag aagaagcctg cgatgactac aatgatacca tttagaatca
     3001 gcatgtagtt tccgactgtt tttccagttg tttgtgaaag tacaagagag atgatatcca
     3061 tcccaccagt tgtaaagccc atttttaaag ataatcctac gccaagacct aataaaacac
     3121 cgcctactag ggcattcatc aaaatattat cagtgatctg tccgactgga agcagcatcg
     3181 tcatcaccga tacacataca acattcacaa aactaaggat cgttgctcgt tttcctactt
     3241 tgagaaaccc taatacaaac acagggatat tcaaaagaaa aataaatgtt cctgtgtcta
     3301 tcttgatatg aaaccattca tataaaagcg aggaaatgat ttgagcgatc ccattcatcc
     3361 ctgcagaaaa gacattggct ggaatcaaaa aataatttaa agcgaccgca gcggtcaatc
     3421 cggttatcaa aataaccact attttcttta atctttcatt tttatgatac gcagtcataa
     3481 ttttactcac aagcacctct cccctacttt ggtattccaa atgtaagaat aacagtttat
     3541 ctattaaaat aaaatacttc gaactcaaat gagaagaatt ttagcaaaca ggtaagaatc
     3601 tgccctaatc ctatgataag atagatgtat ggtaaatatt tatatgtata tcaggagggt
     3661 tcctatggca aaagatgcta gcttcgacat tgtatcagaa gtcaatacag aagaagtaaa
     3721 aaatgcgatt cagcaaacaa caaaagaatt gtccaatcgc tttgacttca aggattctac
     3781 agtagaaatc aaattagaga acagcaatct agttgtattg agcgatgatg atttcaaaat
     3841 cgaacagatc aaagatgtat tatttggtaa gctgatcaaa agaaatgtgc caatcaaaaa
     3901 tatccatttt tccgaatcag aacacgctct aggtggcaaa gctcgacaaa ctgccgattt
     3961 ggtcaacgga atcgatcgtg aaaacgcgaa gaaaatcact acagctatca aaaatgaaaa
     4021 actgaaagta aaagcacaaa tccaagaaga tcagattaga gtgacaggta agaaccgtga
     4081 tgatctacaa gctgtgattg ctttattacg taaacttgac ttaccaatcg agttgcaatt
     4141 cactaactat cgttaatcaa aaaacgaggc tgacgtctgt cattcctcgt ttttttgatt
     4201 tactgcttct tttgataacc aaaaacgctg tattccttct tccaattccc caacacttcg
     4261 tacttctctt gctgtttgcc aatgttgtgg gaataaacgt cgataagaaa aactagaaaa
     4321 tcgttgatct gccaatacaa cgattccttg atccgccatg ccacgaatca ctcgaccacc
     4381 agcttgcaag actttgttca ttccaggaag ctgataagcg taagcaaatc caaggtgttc
     4441 cttctcatca taatatgatt tgatcagttc ctgctcatga ttcatctgcg gcaaacctac
     4501 acctacgatc atactgccga tcaatcggtc ccctctcaaa tcgattcctt ctgaaaaaat
     4561 cccgccaagt acgcaaaatc caatcaatgt ttcagcggga tttgtttgaa attttgttaa
     4621 aaatcgttcg cgttcttcct cattcatgtc cgtttcttgg attagcacat tgctgtcggg
     4681 atattgctgt ctgaatgctt ccaccgcaag atctaaatat tgataagaag gaaagaaaac
     4741 gaaataattc ccgacctttc catcagcaaa agcatgaatc gtctctacta attttgccag
     4801 actgtgttcc ctttttcgat aagtcgtctc taggtaattt gcgatgaaaa cttgctggtt
     4861 tccttctggg aaaggactcg gcagtttgta agccagactt ttttgtcccc ccagtgtttc
     4921 ctgataataa gagagtggtg aaaaactagc agaaaaaaga atactgcttt ttcctttatc
     4981 caacgactgc tcaagaaaca agcttggatc aatacaaaac tcttttacta tcagatcgcg
     5041 atacgttttt tcgactgttg tttcgtagtg atcatcataa aattcactga ccttcaaaaa
     5101 atgaagcaaa tcaaaataat aaggaagcaa ttgttcttgt gatgggtgct caggaaattc
     5161 tgccagccat tctttaattt tttcgcttaa ttgataacct gcctttacta aagattcagc
     5221 cggtgctttt tgatgatgat aatcccaatg atcttctttt gctatttctc ggatcgaatc
     5281 aaattctttt aatagtttgt tgaaacggcg atgaagtttt ttgaattcct ttggtatagc
     5341 ttccttactt cgtttcgttt tttcataaga tagttctgcc gaatacatct ctcgggaacg
     5401 attcaccaaa ttatgtgctt cgtcaatcaa aaacagatag tcctcgtttt tttcatcttc
     5461 aaaaaaacgt cttaaataaa cagttggatc aaataaataa ttgtaatccc caatgattac
     5521 gtcacaccag atactgacat ctaatgatag ttcgaaagga caaagtgtat gctttcttgc
     5581 ataagtttcg atgacttcac gagtgatctg attctcatga ttcaaaagat cccataaacc
     5641 ttcattaatc cgattgtaat aaccattggc atatgggcac tgatctggat tacaggtcgt
     5701 ctcatctaaa aagcagatct tgtctttcgc cgtcaaagtg acacttttta cttcagctcc
     5761 tgtatctttt aatgctgtca tagcatcttc agctacttgt ctagttattg ttttagccgt
     5821 tagataaaag atcttttctc cctcttcttc ccctacagct ttcaaagcgg ggaataacgt
     5881 agagattgtt tttcctgttc cggtaggtgc ttctacaaac aatttctgtt tcgtacggat
     5941 cgttttataa gcagctaccg ctagttcacg ctgacctttc ctatactcat catgggggaa
     6001 tttcaaagcc atcaaagagg cattgcgtac agtgcgccag ttttcttgaa aaacaagcca
     6061 ttctttataa tcttcagtca gctttttaaa aaaagtttct aactgctctc ttgtctgatg
     6121 ttcaaccttt ctcgtgatca cttcttctgc cgtctggaag taagtcaatt gcagatttat
     6181 ttcttgtaaa tcatgttctt gactatagat ataggcatac acccttgctt gatgaaagaa
     6241 aagttccact tgttctggtt ctaaatcttc aaatcgtggt tcggaggttt tgatttcatc
     6301 tatataataa ataccgtctc ttttgaaaat accgtctgca cggccttcaa cgatcaggta
     6361 atattcatct aattgaactg ttgtttttaa aaagacttct ttttcatagt tttctcccgc
     6421 atctttttgg agcttgcggt ggattctcgc tccttctaat gcagtatggt tacttttttt
     6481 acgattgtcg atacttccac gtcgcaaaat aaattctact aagtgccgca cagatatttt
     6541 tgactgtttg atcatttcca ccacctcttt ctctctaaga ttacctgaaa aaacctagtt
     6601 tagcaaaaag agctctcttt ccaaacataa aaaaacaccc tgctgattag gcagggatat
     6661 aggatataga atttggattt ttggatgaat ctaatgtaac aaggacatat taaaatgcta
     6721 tgaaaactta ctttccttct ctttagaata tccaaatact tttcatatag tctgataatt
     6781 cagataagta aagagaaata agtacctgtt tttgtttcct agcaatgaat gcgttatact
     6841 aatggatgaa agaagttttg gctatcaagg aggaatgcac atgcatatcg aaagattaaa
     6901 aacaggaatg attgaagaaa actgttattt ggtctacaac gaagaagcac ttttgatcat
     6961 tgaccctgga gaagatgcgg aacgaatcaa acaagaaatc aaaaaaacaa accaacagcc
     7021 ggtagcgatt ttgcttaccc atactcacta cgatcatatc ggtgcagtgg aacctttacg
     7081 cgcgtattat caaatcccag tttatgtcag cccactagag caaaaatggc taggtgatcc
     7141 gattttgaat ctttctggac tcggtagaca tgatgatatt gctgatgtta ttgtctcgcc
     7201 tgcagaatat gaatttgaaa tgaaaactta tactttagga aacatgtctt ttcgcgtcgt
     7261 ccctacccca gggcattcta taggaagcgt cagctttatt tttgatgatt ttgtcgtttc
     7321 tggcgacgcc ctgtttagag gaagtatcgg tagaaccgat ctgtatacag ggaatttaga
     7381 acaattactt catagtattc ggacccagct gtttgtatta cctgatgaat ttgcagtcta
     7441 tccaggacat ggcgatgcaa cgacgattga acaggaaaaa aggacaaatc cttttttcaa
     7501 tcgttaaaac gacttaggag gagaataaaa tgacagaaac tacactttat atcgtacgac
     7561 acggaaaaac gatgttcaat acaattgaac gagtacaagg ctggtgtgat acacccctca
     7621 caaagcaagg acaagaaggc attcattatt taggtcgcgg gttaaaaaat gtcgactttt
     7681 catttgctta ttctagtgac agtggtcgtg caatcgaaac tgcccgtatt attctaggag
     7741 aacattctaa gggaaaagaa attccttatt ttattgaaaa aaggattaga gaatggtgtt
     7801 tcgggtctct tgaaggtgga tatgacatgg aaatgtgggg tgtgatccct cgtgtattga
     7861 attttaaaac atatgatgaa atgtttacca cagaagtttc ttttgaacaa attgctaatg
     7921 ctgtatacaa cttagatacc gctgggtggg cggaaactta tgaaacattg cgagaccgtg
     7981 tatggacagg atttgaagat atcgcccatc actgcgaaaa aaatggtggt ggaaatgttt
     8041 tagtcgtatc tcatggtttg acgatcgcct ttttgcttaa tctgatcgat cctaaacagc
     8101 ctgttcgtgc tgggctttta aatggcagcg taacaaaggt cacttatgca aacggtcgat
     8161 tttctatcca cggcatcaat gatacgtctt atatcgataa cgggaaaaca catagtcgcg
     8221 actcgtttct ataatgggga tattttaatt tttaccagag aatcataaaa ggcaaagtct
     8281 gaacgagggc tttgtcttct tttttctctt cgtgtactta tctgctttat ttctcaagtt
     8341 tttcttaata ttaaaaaaca gattgggttt ctcaaaaaat aacggtatac tataagatgt
     8401 atcttaattg ggaaggaagg aatttatgtt ttttgcaaat attatcaaag cagtgattct
     8461 cggtattatt gaaggaataa cggaatggct gcccatcagt agtactggtc atttgatcct
     8521 agcagatgaa ttcatcaaac tggatgccag ttcacaattt atgtcgatgt tcaatgtcgt
     8581 tatccaatta ggggcgatct tggctgtcgt cgttctttat tttcacaagt taaacccatt
     8641 ttctccaagt aaatcaacgg tagaaaaaaa agatacgtgg acactttggt ttaaagtgat
     8701 cgttgcttgc ataccagcca tcatcattgg tttgcctttg gatgattggt tagaagcaaa
     8761 tttccacaag ttcttaccag ttgccattgt tttgatcgtt tacggtattg cctttattat
     8821 tgtcgaaaaa cgcaacaaaa acaaaacacc tcgttggact tctctgaatg actttacgta
     8881 ccaagcagct cttcttgttg ggttattcca agtcttggca cttgttcccg gaacatcccg
     8941 ttctggcgca acgatcctag gatctatctt gattggtgct tcgcgttttg tcgctacaga
     9001 gttttcattt ttcttaggga ttcctgtcat gtttggcgca agcggcttga agatcgtcaa
     9061 atatttagct gaagggaatt cattcaaaat ggaagaaact gttgttcttc ttgtaggaac
     9121 attcgtatct tttgtggttt ccgtcttagc tatcaaattc ttactaagct atttgaagaa
     9181 aaacgacttt accgcttttg gctggtatcg tattatcctt ggtatcattt tgattggtta
     9241 ttggtttatt gcaatgtaga aaaaaccaga acacttctct t
//
LOCUS       DARBDV010000104         9273 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000104 DARBDV010000000
VERSION     DARBDV010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.106"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            761..1672
                     /locus_tag="SCM56_002484"
     CDS             761..1672
                     /locus_tag="SCM56_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HEG1111224.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            2159..2425
                     /locus_tag="SCM56_002485"
     CDS             2159..2425
                     /locus_tag="SCM56_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111225.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            2497..2838
                     /locus_tag="SCM56_002486"
     CDS             2497..2838
                     /locus_tag="SCM56_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111226.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            2893..3312
                     /gene="ssb"
                     /locus_tag="SCM56_002487"
     CDS             2893..3312
                     /gene="ssb"
                     /locus_tag="SCM56_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEG1111227.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            3389..3685
                     /locus_tag="SCM56_002488"
     CDS             3389..3685
                     /locus_tag="SCM56_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111228.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            3700..4131
                     /locus_tag="SCM56_002489"
     CDS             3700..4131
                     /locus_tag="SCM56_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEG1111229.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            4313..4618
                     /locus_tag="SCM56_002490"
     CDS             4313..4618
                     /locus_tag="SCM56_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111230.1"
                     /translation="MKLDMPGRYKLLRVRLFLIKLTNLRMYGSITDLYSKDIRLRMQR
                     TLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKRFTEEIEQLAELNIRYF"
     gene            5022..5510
                     /locus_tag="SCM56_002491"
     CDS             5022..5510
                     /locus_tag="SCM56_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEG1111231.1"
                     /translation="MKKTIFVLAIFICFGLLLVGYLLFNSNEMRTISTNDLLKKSELK
                     DKKKESLAFIMFTQEECEYCIAINPIIDSVNKETLNKIKKYDISVDFQSKQKCKIKFV
                     PTIVAYSKNKEVGRIDNELDNLYRKKQDGSIVIDKKKTKTLLIDWMKKMDKQGEYLYG
                     ED"
     gene            6844..7146
                     /locus_tag="SCM56_002492"
     CDS             6844..7146
                     /locus_tag="SCM56_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1111232.1"
                     /translation="MTKLGITLDDNIYDEKILLSGGEKQKISLARALIKSASVIILDE
                     PFRNLDNETRDNLRDYILNTKKEVCYIIITHDHILDNFLDSNDEILYLNKEYDNNE"
     gene            7139..8167
                     /locus_tag="SCM56_002493"
     CDS             7139..8167
                     /locus_tag="SCM56_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEG1111233.1"
                     /translation="MNSKKLQMGEIVRGPSQISIDVTYKCNFRCKHCYNASGGDNCFM
                     DNELTDDELLELTKDICNLKPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTN
                     GSLVTQDVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEE
                     VDVAFCPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLV
                     SDINYIKRNEDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGN
                     IKCHSLSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILD
                     EYKHDILI"
     gene            8223..8441
                     /locus_tag="SCM56_002494"
     CDS             8223..8441
                     /locus_tag="SCM56_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111234.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            8512..>9273
                     /locus_tag="SCM56_002495"
     CDS             8512..>9273
                     /locus_tag="SCM56_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111235.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTELKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYEKFYQDVVGTIKFFKHYNIIDIGDYSME
                     EKKIYKIEQNLELELLNESHYEEIYNFITDHPESMYFHEDIYHQGNSKELEVVLRYNL
                     FSFTSEFYFLRHKERIVGMVSIIVNPFQNLSYSIVNYLICEPAYTTTILTTVLENINE
                     VSILEVKKLVIHLLNSTEDESLIPYLKDSHFFKEAV"
BASE COUNT         3493 a         1213 c         1765 g         2802 t
ORIGIN      
        1 agctaaaatc aatagaaaaa ataaacgtat tttgtttgaa atccgtttat tttttttacg
       61 tttcagtatt agtaaggatt atttgagggt tatttagaga ttatttaaag gttatatgta
      121 ataagtgggg ggagggctgt cggcgcttgg tatgaaaggc attctccgac ttccaggttt
      181 ttgaccattt tctctcaacg tggaaaaacc gccacttatg agagaaaatg gtcaaaaacc
      241 taagcgggaa gtctccgaat aacataccaa gcgccgacag ccaacagata aagaatacac
      301 tactaaaaat agtagtagca taaaatttaa aagggaaaaa atcaacccct accccagtct
      361 tggggtctca aaactaactc ccccctaacc ccctctttca gcctcttttg aaagaggggg
      421 agaatcaacc ccacggccaa cgccccagtg ttcttggggc tgtccagaac agaaacttgc
      481 gagagagtgg gggtaaaaca agtgtagagg aaacttgaag acttgttcga acagaaactt
      541 gcgagagagc cggagtaaaa caagtgtaga ggaaacttga agacttgttc gaacagaaac
      601 ttgcgagaga gttggagtaa aacaagtgta gaggaaactt gaagacttgt tcgaacacaa
      661 acttacaaga gaggggagta aagcagttgc agaggaatca ggaagatttg ttcgaacaga
      721 aacttgcaag aaagcattat taaattgaag gtgatgaaat atgaagtatt tattgcatgg
      781 ctattttatt gattattcta ctcgcaatag tagtacttta gcgattctat gtttgcttca
      841 tgaattgaga gtggttacta gaaaacagct atacaaacta ttgaattttg acagatcaat
      901 tagtaaacaa ggagtaacga atgctttagt gagattatat gaaaaacagt taatcgatcg
      961 aaaaaaatat ggaacagaaa tgtattatta tttgacgaaa gagggacatc aaagcatagg
     1021 tgggtattat acgttgccga aagttcctga atataatttg aatcaccatt tacaaattaa
     1081 caattatttg attaaaggaa tggcattggc aaaagaccac ccacatttaa aacggattat
     1141 ttcagaaaga cgtaccgtct atgaaacgaa ggatttaaat cctaagacaa aaggaagaaa
     1201 attttttgtg ccagatttta tttttcgttt tcgagacaaa gaaaatcatg agatcaattg
     1261 gtcctttgaa attgagctaa cgttgaaaac acgtcgtcgc taccgtgaag cgatttttcc
     1321 gaaatatatt cgtgagttgc gaacacgtcc tgaatcacat ttaatttatg tgacacctag
     1381 tggcgcaatt caagaagaac ttcatgcgtt taagcgtatt tttttcaagc gcgaaactga
     1441 aaaagatgcg agtcaacaag aattgtttga gcggttgcat attttttcag ctttagagtt
     1501 tgaagaggga ttaaaagcat tattagagaa tgatccagca gtcaattggc ggacagtaga
     1561 acgagaaaaa atttatccga atggcgctgt tcaaagtcgt ttgaatttat ttgaagaatc
     1621 aactgaaaat gaggaaaaac caaaagtgaa gtttacgcct gaagtattgt aaaagagggt
     1681 gttcgcttcg ctcttttttt gtgccagttt gctggcaccg ttcagacgca agcgtctgaa
     1741 ccggctcgtt tttaggaagg actaacttgt ttgatttatc cgttgtcaaa gaagtggacg
     1801 ttcgagagtc cctaatcgta tagcagtcat tcggcgagta taacctaaaa cataacctgc
     1861 caattgccaa gggtaaaatg cacgctgcgc gcccttggca atcgtcagaa catgttttcg
     1921 agccggttat gccgaacgac cgctatacga aaagggcctc taaacagaag tccactttga
     1981 tccttgacaa ctgaaaccca aaatcaaaca agcaggcctt cgtctaaaaa cgagccgaat
     2041 gaagaccttg ggggttgcac ccccaaaccc ccgtgccccc gacacactac cgaaaaggtg
     2101 ggggtgggtg gcgaaaaaat caaataaaac attctattca agaaaggaag aaacaaccat
     2161 gacaactaaa aacgagcaac tgaaagaaca ggcaagaaaa gagttgcaag aaagaggttt
     2221 gattattgag gggagctttg aaggagattt tgaaacctat attggttgtt atgcaagacc
     2281 gataaacaaa cctacagcct tagacccaat gaacgaacaa gaagtaaaag aacaagaaaa
     2341 acatgcagtg aatggttttc cgcaagattt cactgaatgg tatgagtggg aaattaacaa
     2401 cgggaagctt gaaaacttct tataaaagtc agatgacgtg caaagagaat gattctcttt
     2461 gtacggtaaa aatataaaga aagaaggaaa aagaaaatgg caaaaaacac accttatcaa
     2521 gtaggagaca ttttttatag tagttggggc tacgaacaaa cgaacgtgac tttttggcaa
     2581 attgtgaagt taacagaaaa aacagcatgg tttcagccta ttcaaaagaa aacggtcgag
     2641 cattattcgt ccatgagtgg aaaagttgtt ccaatgaaag acaagtttat agagtatcct
     2701 ttagcaaaaa ctaaagacag tattgtaaaa aaacgaattg atccagaaac ctatcctgat
     2761 atgttatgtg gttatcgtag ttgggacacg tttcatagat ataaaaatca agctgtttat
     2821 gaaagtagtt attattaagg ggatagtttc ccctttcaaa aataataaat agaagaatgg
     2881 agagaaaaaa tcatgatgaa ttcagtgaat ttagtaggac ggttaacaaa agaagtggat
     2941 ttaaaataca caccaaaagg caatgcgaca ggcacattta ttttagctgt taatcgaaat
     3001 tatacgaatg caaacggcga gcgtgaagca gattatatcc gctgtgtcat ttggcggaaa
     3061 gctgcggaaa ctttagcaaa atatactgga aaaggaagtt tgattggcat taatggacgt
     3121 cttcaaacga ggagctatca gaatcagcaa ggacagacca tgtatgttac ggaagttcta
     3181 gtgacagatt tctatttgtt agagagtaaa gaaatcaatg agcaacggtc aaaatcaatg
     3241 gcgaataatc aaaataattc ttcaattcca ggaaatgaga ttcaaatttc agatagttat
     3301 ttgccctttt aagtgaaaaa taattttgtt ctatacctga tttcttcagg tatagaacaa
     3361 aaaataaata gagatgaggc gattttttat gaaaatcatt gacagttacg atcaacgaac
     3421 catgcaatat ttgggctcgt ttgattatac agatgaaaat attattaatt atgttgcaag
     3481 tttacctcct tatcaaagcg ttcgcttggt agattttgca agtgatgaat tggtattaac
     3541 aacgatgggc aactttttag attatgtcgg agacaaaaaa tggttggaaa aaattttgtc
     3601 taaattgatt gcgaaacaaa caggagaaga acccataaat gcgattcaat tgttcaatcg
     3661 atatgaacaa gaaaacaatg attaaagagg agagaaaaaa tgaaaagatt aaaacgaagt
     3721 atttttattg tgctagcatt gattgatttt gttgggacag gttgtgaagc aattgacgtt
     3781 caaaaagctc ccttatcgga aataaaacaa gcaataacaa aacaagatga agaacaaccg
     3841 ctaaatacac aagtagaaat agtgaatcaa aataaaccga gtttttctga agaagatttt
     3901 tcgttagaaa atggaaccag gcaatttttt tcagatttgg atcgtttaaa tcgggtaggt
     3961 gtagcaagtg cacttttgca taaaagcagg atgccaacag aagaacgaga ccgattatac
     4021 attaagccaa ctggctggaa acaaaagaaa atgaagaatg gcgattatct ttataatcgt
     4081 tgtcatttga ttggctatca acgtgcgaca agtttcccca taagtatgta atacgaaacg
     4141 ggaggattaa tcttaagatt aatcacaact aacctatttg acagttggaa tgaaagctat
     4201 catgtctgct gaatcaactg gtctgatact cctacatgca ataaagtcta ctaggaagtt
     4261 attgtatgaa gctaggtaaa ggcggcagaa caatttctaa gtgtggaatc atatgaagtt
     4321 ggatatgccg ggacgctata aattactcag ggtgagactg tttttaatta aactgacgaa
     4381 cttgcgaatg tacgggtcta taaccgactt atattcgaaa gatataagat tgcgaatgca
     4441 aagaacatta tcgaaaagta agaatgttgg tcatgaaatt cgagatggaa gactagcggt
     4501 gtatctgtca agagttggta aaacatgttt gattgattca gatgagagtg ggaatgcgac
     4561 aaaacgtttt acagaagaaa tagagcaact agcagaactg aatatacgat atttttaaat
     4621 actttagcgg agaataaaaa aatcctctac agaaaactac ggagggcaac atgtgctatt
     4681 gtttcaacaa tacattttta atttttacca taacaaaaaa gtacttgaaa aaaaacagaa
     4741 atatgttaaa ttaaaaatga aaaaatggaa gcggttactt attcttttga atctaagata
     4801 aaaattcaaa agaataacat aacccaaata tgattatact tgagattcta aatatttatc
     4861 gaaaaggaat ttgttttaag gaaaaatgat tctatggacg gttattgatt tatatttacc
     4921 aaatggctaa taaaatagta tttcttatat tgtatagttt ttgtttttat gtgaaaacat
     4981 gagcaataga attgtactag taggataagg atggatggaa aatgaaaaaa actatttttg
     5041 ttttagcgat ttttatatgt tttgggctac ttttggtagg ctacttgttg tttaattcta
     5101 atgaaatgag aactatttct acgaatgact tgttaaaaaa aagtgaatta aaggataaaa
     5161 aaaaagaatc tcttgccttt ataatgttta ctcaagaaga gtgtgagtat tgtatagcaa
     5221 taaatccaat catagatagt gtaaataagg aaactttgaa taagataaag aaatatgata
     5281 tatctgtaga ttttcaatct aaacaaaagt gtaaaattaa gtttgttccg actattgttg
     5341 catatagtaa gaataaagaa gtggggagaa ttgacaatga attagataat ttgtatcgaa
     5401 aaaagcaaga tggttcaatt gtaatagaca agaaaaaaac aaagacattg cttatagatt
     5461 ggatgaaaaa aatggataaa caaggagaat atttgtatgg agaagattaa acagacattt
     5521 ccgttaatta ataaatattg gaataagaaa aaaattttta tattttttat tacgtttctg
     5581 ctgtcatctt tattttctct aattttcccg tatctattta gtaatgttat tgatttatat
     5641 ttagagaatg agttatcaca gaatattgtg cgaatagtaa cgattcttat atttcagtta
     5701 tttttagtct taattaaaga agtgtatgga tatataaaaa ctatttatgc atttgaagtt
     5761 gatttatcaa tgaaaattga ttttcttaag gttttacaaa aaaaaaatct ttttttttcg
     5821 caaacgaagc attcaggaga aattcaattt aggatgtttt cggatatttc tactatcgta
     5881 aatactatct ttattttttc tgttgaatta cctgtgatag gcgttttagt tcttgtagcg
     5941 gtatttatta taagtaaagt gaatcttatt tttctgattt tttcactaat aatgatggga
     6001 gctatggtgt tacagtataa tttatttcaa ttaccgctta agaaaacaat cgagaataca
     6061 aaaagtgtta atcaaagtat tacaggagaa ttgaatgcac attttgatca attagaaatt
     6121 ataagcgcat ataattttga gaatatctct attttaaaat tcgattctag atttaaagaa
     6181 ttatttaatg ttttcaaaaa acaaaataaa ttactaacac tgggaagctg tttggcacag
     6241 gttattttcc aattatggat tggaggaata tttcttattg atgttatttt attagctaaa
     6301 ggaacaataa gtattactaa ttttgtaata gtatttatgt acgctaacat ggtccctact
     6361 ttatggacta ctttaattag tattatttgg aaatggcaag actattcagt gtcattaaat
     6421 agatattacg aatattattc aaataacaac aaagtggctg gcacaaaaaa aataaataaa
     6481 ataaattcta ttactggtca atcattaaat ttttcatata acaaaagaaa ggtattaagc
     6541 aatataagtt tttcaactga aatgaataaa ctattcatga tatcaggaga aaatggaaca
     6601 gggaaaacaa cactgatgaa attatttact aaacagttgc cggttagtaa taatcaatta
     6661 ttcgtcaacg ggatagatat taatatgatt gataacgaat cattaagaag gttaatttca
     6721 atcgcaacac aaataccata tataattaat agctccgtga aggagaatat tttaatgggg
     6781 actaaaaact atgataaaaa aaaattcagt aaattaatag aactattggg aataagtaaa
     6841 tttatgacga aattaggtat tactttggac gacaatattt atgatgaaaa aatattatta
     6901 tctggaggag aaaaacaaaa aataagttta gctagggcgc ttattaaatc agcttcagtt
     6961 attatattag atgaaccatt tagaaattta gataatgaaa ctcgcgataa tttgagggat
     7021 tatatactaa atacaaaaaa agaagtttgt tatataatta taactcatga tcacattcta
     7081 gataattttt tggattcaaa tgatgaaata ttatatttaa ataaggagta tgataataat
     7141 gaatagcaaa aaacttcaga tgggtgaaat tgttagaggt cctagtcaaa tatctattga
     7201 tgttacatat aaatgtaatt ttagatgtaa acattgttac aatgcgagtg gtggagacaa
     7261 ttgttttatg gataatgagt tgactgatga tgaactgtta gaacttacaa aagatatatg
     7321 taatttaaag ccatataata tttgtttctg tggaggagaa actttactga gaaaaaaaat
     7381 agttttatct tgtgccactt tattaaaaga ggcaggtatt cctaatattg ctattgttac
     7441 taatggatca ttagtaactc aggatgttgc aaaagaatta ttcaaagcag gtattaatcg
     7501 atttcaagta agtttagatg gaaaaaatgc tagtagccat gaagcactta gattatatcc
     7561 aaacagtttt gaaaaggcaa ttaatgctat caaattattt agagaattgt ccgaagaagt
     7621 ggatgttgct ttttgtccta ctagttttaa ttacaatcaa ttacgagaag tatatcaact
     7681 agtaaatact cttaaagtga agtctttaag aattcaacca tttatgctta gtggtagggg
     7741 aatgcaaaat agggaaatag tgccaactgt ttctcaatat agaaaactag tttctgatat
     7801 taactatatt aaaagaaatg aagattatac caagatagat tggggagatc caattgatca
     7861 tatcatccga cacagaactt tattgaaaga tttttcgaat tttgtttcta ttaggtcaga
     7921 cggaggatta atggcatcac cttacttgcc aattcaagtt ggaaatatta aatgtcacag
     7981 tctttctaaa tattggcgag caggacttta taaagttttt tctttagatt tagttcaaga
     8041 tatagctact caagtttctt caatacaaga tatgagtaaa gaattaaaaa aaggacatat
     8101 taaatggctt aatgaagata tagaaattga tattctcgat gaatacaaac atgatatttt
     8161 aatttaggtt tatcattgga aagtttagat aaacaaagta cttttagaaa ggagggaaaa
     8221 aaatgatttc tgaacctcaa atgaataata tgtatgaaag cccaactata atgggagctg
     8281 gtgaagctgc gccaagagct tgttcagcaa ttatggtgtg tgtgttatta gcatctgcag
     8341 gagtaggtat ccaagcagga gcggtgtata cttatgctgt agttgctaca gtagctgtag
     8401 ctgcagccgc agtttggaaa acagtaggat tagttggtta ggaattaata ggagactaac
     8461 tgtctcctat tgattccaat gattaagcta aggaggaatt ttaagaaaat aatgaaatac
     8521 aataatttaa aaccgaaatt acttaatgag aacatagaat atataagaga agaaaattat
     8581 ggatatttaa ccatagttcc caaaaatgga actgaattga aagataacgt tttcaatcca
     8641 acagtaagat atatattggc taactgtact ggaaaaaaca gtgtaaaaaa aatagcgaat
     8701 ggtttaaaaa agaagtttcc tagtttagaa tatgaaaaat tctatcaaga tgttgtagga
     8761 actattaaat ttttcaaaca ctataatatt attgatatag gagattatag tatggaagaa
     8821 aagaaaatat ataaaattga acaaaattta gaattagaat tactaaatga atcacattat
     8881 gaagaaattt ataattttat cacagatcat cctgaaagta tgtattttca tgaagatatt
     8941 tatcatcaag gaaatagtaa agaattagaa gttgtcctta gatataattt attttcgttt
     9001 acgagtgaat tttacttttt aagacataaa gagagaattg taggaatggt ttctataatt
     9061 gttaatccat ttcaaaactt atcctacagt atagtaaatt acctcatatg tgagcctgca
     9121 tatactacga ctattttgac aacagtatta gaaaatatta acgaagtttc aattttagaa
     9181 gtaaagaagc tagtgattca tttgctaaat tcaacagaag atgaatcact aatcccctat
     9241 ttaaaagatt cacatttttt taaagaagcg gta
//
LOCUS       DARBDV010000105         9265 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000105 DARBDV010000000
VERSION     DARBDV010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.107"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            476..1339
                     /locus_tag="SCM56_002496"
     CDS             476..1339
                     /locus_tag="SCM56_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEG1111236.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="SCM56_002497"
     CDS             complement(1468..3090)
                     /locus_tag="SCM56_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1111237.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="SCM56_002498"
     CDS             3339..3860
                     /locus_tag="SCM56_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEG1111238.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="SCM56_002499"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="SCM56_002499"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEG1111239.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="SCM56_002500"
     CDS             5941..6759
                     /locus_tag="SCM56_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEG1111240.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="SCM56_002501"
     CDS             complement(6862..7059)
                     /locus_tag="SCM56_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111241.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="SCM56_002502"
     CDS             7228..7806
                     /locus_tag="SCM56_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEG1111242.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="SCM56_002503"
     CDS             7901..8038
                     /locus_tag="SCM56_002503"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111243.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(8851..>9265)
                     /locus_tag="SCM56_002504"
     CDS             complement(8851..>9265)
                     /locus_tag="SCM56_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEG1111244.1"
                     /translation="FLTYSYSNVNLVPFSTFSFENTTVLRETTYNLIVFFPFGVLLNV
                     NFKRLSFSKKLGIIFLVSFLAELIQFLFGIGVADITDLITNTTGGLIGLWAYQLLNKH
                     LSTNKLDRLAIILGYILFLFLIVAFLFLTRTVRLQ"
BASE COUNT         2877 a         1628 c         1803 g         2957 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgttttc aaacagtata aaacggtgtg gtttttggaa taaaaaaata acatatctca
     8341 ttttacgctt ttgttatttt atacttaaaa aataacgaca cagaaaatac cgtgtctcag
     8401 atttctctac tacttctaaa aactactgtt tttttccttt gaaatgcctt caaaaagtag
     8461 gatcaggcag aatatactgg cagaatcaag gagaagggag cctaaataag gagaaaaacc
     8521 atagaaaata taaagtaatt acttataaaa caagcttttt atagaaaaat aatatacctg
     8581 agtagggtat aaattaagac ggaattcctt tctagattga gatattgact aggaagaatg
     8641 ttaagatttt aagcctttaa gctaaaaaaa aacccttttt gttaaataag gggaatatag
     8701 cctgtcagtt tcaaactgca agtcgtcctg ctgcaataat atagtagatt agagatgaaa
     8761 atagattttt ttaaaccaat gactgaataa aaagaccgtc atagatacac attggaatgt
     8821 gtctattacg gtcttttttt acgaacgatt ttattgaaga cggacagttc gggtcaaaaa
     8881 taaaaaagct acgatcagaa ataagaataa aatgtatccc aaaataatgg ctagacggtc
     8941 taacttattt gtcgatagat gtttgtttag aagttgatag gcccataaac ctatcagtcc
     9001 tcctgttgta ttagttatca aatcagtgat gtccgctacg ccaataccga ataggaattg
     9061 aatcaattct gccaagaagc ttacaaggaa aataatccct aattttttag aaaaagaaag
     9121 tctcttgaaa ttgacattca aaagcacccc aaaaggaaaa aagacaatca agttataagt
     9181 ggtctcacgt aataccgtcg tattttcaaa agaaaaagta gaaaaaggga cgagattgac
     9241 attgctgtaa gaataagtga gaaat
//
LOCUS       DARBDV010000106         9152 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000106 DARBDV010000000
VERSION     DARBDV010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.108"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(371..883)
                     /locus_tag="SCM56_002505"
     CDS             complement(371..883)
                     /locus_tag="SCM56_002505"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEG1111245.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(897..3194)
                     /gene="recJ"
                     /locus_tag="SCM56_002506"
     CDS             complement(897..3194)
                     /gene="recJ"
                     /locus_tag="SCM56_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEG1111246.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3336..3779)
                     /locus_tag="SCM56_002507"
     CDS             complement(3336..3779)
                     /locus_tag="SCM56_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HEG1111247.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3780..4568)
                     /locus_tag="SCM56_002508"
     CDS             complement(3780..4568)
                     /locus_tag="SCM56_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG1111248.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4565..5506)
                     /locus_tag="SCM56_002509"
     CDS             complement(4565..5506)
                     /locus_tag="SCM56_002509"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEG1111249.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5683..6270)
                     /locus_tag="SCM56_002510"
     CDS             complement(5683..6270)
                     /locus_tag="SCM56_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEG1111250.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6491..7798)
                     /gene="obgE"
                     /locus_tag="SCM56_002511"
     CDS             complement(6491..7798)
                     /gene="obgE"
                     /locus_tag="SCM56_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEG1111251.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            8092..9102
                     /gene="gap"
                     /locus_tag="SCM56_002512"
     CDS             8092..9102
                     /gene="gap"
                     /locus_tag="SCM56_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEG1111252.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
BASE COUNT         2536 a         2090 c         1603 g         2923 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaacttttac gggggtgtga caaaaatcgg tcacatcctt tttttcgaat aaacggtgtt
      181 ctaaagccag tttttcggta ttaagtcaaa ttgcggaaaa agtgattttt taaacttcta
      241 ctggtctttg cttgtttcca tttataaaaa aatagcgacc ctcaaaagtc agatagagaa
      301 tctgactttt gagggtcgct ttaaaatcgc ttctttaaga ataacatcaa gaatagcttc
      361 ttttttctgc ttaatattcc attaatgtaa tgatgtcata tcctttgatt ttgtcgcgtc
      421 catggagatc catcaattcg atcaaaaatg cgcagcctac gactacaccg cctaattgtt
      481 cgatcagttc gatcgtcgct ttgattgttc ctccagtagc tagcaaatcg tcacaaatca
      541 gcacacgttg acctggttta atggcatcct tatgcaatgt caatgtatct gttccatatt
      601 ctaatccata agtcacttcg atggtttctc gaggtaactt cccttttttg cggactgggg
      661 caaaaccgac tcctaactca taagcaacag gacatcctac gatgaatcca cgggcttctg
      721 gcccaactac catatcaatt cgtttttctt ttgcataatt tacgatttgt ttagtagctt
      781 cccgataagc gtcaccatct gccatcagtg gggaaatatc tcgaaacaca attccttttg
      841 acggatagtc gggaatactg gcaatataat cttttaaatc cactctttgt ttcctcctag
      901 gttgttaacc atttctttat ggtaggaata tcgctcaaaa gcaaaaattc ctcaactttg
      961 attttattga gccgtcgctg gtataatctg ctctcagtca aaggatgact ttctgggcta
     1021 gcgacttttt gtaaaacacc gttctctatt gtaacaaatt tcaattcaaa aaacacctgt
     1081 atcataaaaa ttaataattt ttcagggata cgcaaatatt gagctattgc ttttatttta
     1141 tgacgtatat caattcgttc ttgagaatgg atcaatttaa acaattttgc gtactgttct
     1201 cttgtgccta ccccattcaa ataggcctca tcaggtgaaa tccccatcag ataaacacga
     1261 ttgaatgaat aagaccgaaa aacttctttc agatccgcta aatcatctgg acaatccata
     1321 acaactagag agtttggttt tcttttttca attattgcct ttacagaatc tagtccttcg
     1381 aaaacaatcg cagcttcctg gataatctca ggcacgaatt tcaacgtcga agaatcaaat
     1441 gctaaataaa ggctaccttc cgacgttcgg ctctgttcac ggaaacgttt tgcccgccaa
     1501 tcaaatactt gcagaccacc cacagaaaaa tcagatacca tcagctgcgg tttcttacgc
     1561 ccgttccatt cattgatcga aagctttcct accacatcta cctgattatt cagcagttct
     1621 tcttcttgag taccaaaacc aaaagcgacc gcatcaagct gactcgatgc atcggaaagg
     1681 acaaatttca gatgctgttg gtttgctccg attcttttga tattttctgc ttggacttgt
     1741 cgaaataaaa actgaggcaa aggattatcc gtaccaaatg gcgccaatag tttcagctca
     1801 tcaattcgct ctactgttac ttcatttggc agcaatactt catcaattct caactcaggc
     1861 cctctcatca agtcaatttg atgatcgact atatattggt tcatcttctc ctgtagtata
     1921 gtgacatttt cacttggcat cgttaaaccg actgctgcat gatgtcctcc gaagtaagta
     1981 aataaatctc gcatctggtc aagcatttca aaaagattca aggcttcaat actacgtcca
     2041 gatcctttag ctgatccatc ttcttttaaa gtaaggacaa gcgttggttt acccgtttca
     2101 ttcattattt tcccagcaac aatgcccaaa acgccttcat gccatcctcg gtttaccaat
     2161 agatggatat gattctcctt attgaccatc gctaatgctt cttgtgttat ctgctctacg
     2221 attgcttttc tttcttcatt gatctcattc aatttttttg cttgaacgga tgcttcctct
     2281 tcatcaaacg tcgcaagtaa cgagacggca ggacgtggat cgcccatccg accgattgca
     2341 tttagtctgg gagcaatcga aaaaccgatc gttgtttcgt ctgcgtccct catttttacg
     2401 ccgctttctt caaatagcgc ctgaagtcct atccgttctg aatgatgaat ggcatcgatt
     2461 cctaatgaaa caaggattcg attctcatca gtcaatgaaa ctaaatcggc gatcgttcct
     2521 atcgctacta aatccaaaaa ttctgcaggc ggttcttcca ataatgcaga agctactttg
     2581 aatgctactc cgactcctgc caaatctgga aatggatagt gccccgctgg atgacgaggg
     2641 tgaacaatcg cataagcgtc tggtaaattc tcaggtagtt cgtgatgatc tgtaatgatc
     2701 acgtctacac cagcttgctg agcgtatgct accgcatcat ttcctgctac cccgttatca
     2761 acagtgatga tcaactggat tccttcttcg atcttttctt ggtataccga ttggtttggt
     2821 ccatacccat gctcaaatcg atttggcagg aatacttcta cttctgcacc aagtaattcc
     2881 agtgtttctt tcattaccgt tgcactggtg atcccatctg cgtcataatc accgtaaacg
     2941 aggatttttt ctccactgat tacagcttct tggattcggc tgattgcttt atccatatcg
     3001 tgtatcagat acggatcatg taattcttgt tcctttggat gtaaaaatcg gttgatatcc
     3061 ttttcctcat ggtaacctct ttgccacagt aacttaccga caaatggaga taatttcagt
     3121 tcacggatca tttggagaaa ggattcagaa ggctctgtct cgcttaactt ccaatcaaaa
     3181 tttgcttgtc tcactttctc actcctaact tagttattat agcaaaggaa atcagcaaaa
     3241 aaaagcagaa agaaagaata aaaacagatc agatccgcta gacagatgtt cagcaggacc
     3301 tgatcttttg tacgaccatt tatgaaatat ccctattagt caaaataatt gaagctttgt
     3361 ttgggcgatt gagttttttg ttcttccggc gctttcaatt gactgatttg tgcggttttc
     3421 gcttgtaact ccgcctgata agcagcttcc aactccgcac gttcgtttct aaactcgcgt
     3481 tgctgttctt cttttgcttc ttcaattttc ttttgaagtt ctgtttgata ttctgacatt
     3541 tcctgagtca gctgtttgat ttgctttttt tgctgccata gtgttgccga agcagtcaaa
     3601 aatacaatca gtgcaccaat cagcgcagaa ccgatgatca ctaagatcaa tggcgcagaa
     3661 aaggaagtga agccaaaact tacaggaaca tttttattgt tcaatacagc aaaaagaaca
     3721 ataatcaaaa ctaaaacaaa tccaagaatc acattgcttt gctttttcaa ttccttcacc
     3781 tactttttgt taaaaattcc gctggctaaa taatcaccaa tcgttggaaa cagcgtataa
     3841 aattttgatg caacttctaa aataaacggt tgattgattt cccgttttgg atgtctcata
     3901 ttgcggacga tcatttttgc caacttggca ggttctaaaa cgatttgtcc gactttttcc
     3961 aaatagcttc cgcttggatc agccttatcg aagaaatttg tctcgattgg acctggattc
     4021 actgtcgtaa cagctacgcc aagtggtttc aattctaatc gcaaagcgtt ggagaagcca
     4081 aggactgcaa atttcgtggc agaataaacc gttgattttg cagttgccat ttttccagcc
     4141 atagaggcga tattaatgat atgaccttgt cttctttctg ccatatcgat cgcaaatttt
     4201 tgtgtcaaaa ccatcattcc taagatgttg acttcaaaca tatcgtatgc ttttccaagg
     4261 tcgaatgtaa ggaagtcttc gaaaatccca aaacccgcat tgtttaccag aatatcgacg
     4321 cgcccgactt cttccatcac cttttcatat aattcgtcca cgctttcagg attgctgacg
     4381 tctaccttga aagcataagc ctctttgccg ctcaagcgtc tgcactcgtc tctgacacct
     4441 tctacaaatg ccattcttcg tgcgcaactt ataacgattg ctccttgctt tgctgcttca
     4501 taacagattt gtgcgcccag accgcctgaa ctacctgtta ctaaaacaac tttatcttct
     4561 aaattcatgc ctcttcctcc tcgcgaaatg gaatctcaat gctatcaaaa tctttcataa
     4621 tcgtcgtgtt ttcaaacact tcttgtgctt ctttttcaag ctctaaagct gcttttccta
     4681 aatatctcgc actgatatga gtaagtagca gccgttttgc ctgcgcttct tttgcaactt
     4741 cagcagcttg atgagtcgtg gaatggaaat agttgtgcgc cattcttgct tcatctttat
     4801 taaatgtgct ttcatgtacc agaatatcgc tattttccgc aagcacaacg gaattatgtg
     4861 tttttctcgt atcgcctaaa atagtcacga ttcgtccttt tttatctgga cctacgaaat
     4921 cttttccatt gatcgtccgc ccatcttcta gttggatcgt ttcaccttgc ttcagtttgc
     4981 cgtacagtgg tccagctgga atacctaatt cttttagacg atccacctgc aattctcctt
     5041 tatgatcatg ttcaaccaca cgataaccaa aactgtcgat cccatgattt agtttttttg
     5101 tataaacaga aaattgctga tcagtaaaaa tcggggctgt ttcatttagc tcaatgaatt
     5161 tcagcggata acttaaacgc gtttgcgaaa tacctaaaga gacttttatg tattcctcta
     5221 tcccttttgg cccgtaaatc tctaatggcg tatccccacc ttgaaatgac cggctgctga
     5281 tgagcccggg aagtccaaaa atatgatcgc cgtgtaaatg tgtaatgaaa attttaccga
     5341 tttttctcgg acgaatagtc gttcgcaaaa tctgcatttg cgtgccttcg ccacagtcga
     5401 acaaccaaac ttcattgcgc tcgtctaaaa gtttcaatgc tatacttgtc acatttctat
     5461 gttttgcagg aacacctgca cctgttccta aaaattgtaa ttccataatc tacctgactt
     5521 tctttaagtt cctctttaat tttagggtaa aaagaaggga taatcaaccg taagtcgaga
     5581 atgggttcaa attaaaaata acgttctaaa gcgatagcgt tttctttgaa aattactata
     5641 aaaacagcag cgaacccgtt tgagatcctc tgctgttttt tattaatttg aagatccaga
     5701 actttcattg tcgttggaag tttgcggatg tttgtccttc caagtctttt gtgcagcttc
     5761 tttcgcatga ttttcagctt ctttcgcatc atgcacatct ttttcgacat cttcataatc
     5821 caagcgagta atttctccgc ctaattccat cattaccaat tgattcagtg ggcgattgtc
     5881 ctcttcatca gcaatcaaga tcagtcctgt agctccttca ccgattttat ttcctacaaa
     5941 gtcgaacacg gtctgggcat cttttatttc tttggcatct tgtgtcgcac cgatcattcc
     6001 tccggcaaac catcctaata aaatccctag cggaccacct aatacaccaa ccaacattcc
     6061 gattaatcca cccgtagaag atttgttatt accggtaaag tcgataaaat cttctatttt
     6121 gaattgatgt gtcccattag gatcatggct gacaactgcc atctgttctc ctttaatcga
     6181 acgaacagca tgtaactttt tgatttctga aaatgcctga tatgcctggc tttctacttg
     6241 aaaatttaaa atgattactc gtttgctcat taaaaacgcc cctttcatac cttcttgtta
     6301 tttattttac ctttaattga aaaataagaa aagaaaagca ctcttttttt ccaataaaat
     6361 agggctgtga caaaagtcgt atcacagctc ctttttccga ataaacggcg ttcttgaagc
     6421 tgatcacttc tgtcacaacc tcccttaaat ctgaatctat attttttggt atttgttttt
     6481 atttgcttgc ctattcgaca aattcaaatt caaactcacc gatacgtaca aggtcgccat
     6541 ctttcgcacc gcgtgcccga agagcttcat ccacacccat accacgtaat tgacgagcaa
     6601 aacgcatcac tgtttcatca tgatcgaaat tcgtcatttg gaataatttt tcgatcttct
     6661 caccagataa gatccacgtt gcatctgaat cacgatcgat ttggaactca ggaccttctg
     6721 gattgaatcc atagtgtaca gtttcttctt ccagttcttc ttcgtacaat gggaattctg
     6781 gcgttacttc taacaaatct gctgtagcat tcaataacgc gtccaatcct tgtcgagtca
     6841 ctccagaaat tggaaatact gggatgtcat cagcaaattc atcttctttt tccttgttca
     6901 gctgttcttt gaatttcact agattttccg gtgcatccgg catatccatt ttgtttgcca
     6961 cgatgatttg cggacgctct agcaagcgta agttataagt agataattct ttgttgatgg
     7021 caagatagtc ttcataagga tctcgtcctt ccatcccact catatcgatc acatgtaaaa
     7081 ttactcgtgt tctttcgata tgacgaagga attgtgtacc cagcccaaca ccttgcgaag
     7141 ctccttcaat caagcctggc aagtctgctg cggcaaaact tctgccatca cttgtagtaa
     7201 ccattcctag attaggtacg agcgtcgtga aatgataagc gccgattttt ggacgagcag
     7261 atgaaataac ggatagtaaa gtcgatttcc cgacagatgg aaaacctaca agtccaacgt
     7321 ctgccaatac tttcaattct agttcgattt ttcgctcttg tcctggttca ccgttttcag
     7381 caatttccgg tgcaggattc cgaggagagg caaaacggat attccctcgt ccaccgcgtc
     7441 caccttttgc cacaactagt gtttgaccat tttcaattaa atctcctaaa agagcaccag
     7501 tctccgcatc tcggacagtg gttccttgtg gtactttaac ataggtatct tctgaaccac
     7561 gaccgtgcat ccctttactc atcccatttt cacctggctg cgctttgaaa tggcgattga
     7621 accgaaaatc catcaatgta cgtaaccctt catcgacgat caagatgaca tcgccgccac
     7681 gaccaccgtc tccgccagcc ggtccgccgt caggaacgta tttttcgcga cgaaatgcaa
     7741 ccattccgtc tccgcccttg ccagctttca catcgatcgt tacttgatct aaaaacatgg
     7801 acataattaa gtcctcctat tattcattta tttatttgac tgcctgaatt tccacaataa
     7861 acaaaagtct tacttatttt atcggtgaaa tctgattttg tctatcattt cagcaaaaag
     7921 attgtttacc ttcttctttt tttatcattt ggtaaggttt ccaacctttt ttctgctacc
     7981 aagtataatt ttttgacaaa atattctttg cgtagcagaa taacagtgta ttaattgtat
     8041 taattgagga tgccgtttca cagcacccct aataaaggag gaacttttga tatgacagta
     8101 aaagtaggta ttaacggatt tgggcgtatt ggccgtctgg ctttccgccg catcaaagaa
     8161 gtctcagatg atattgaagt agtagcaatc aacgacttaa caagtccaac tatgttagca
     8221 caattattgc aatttgactc tactcatggt acttatcctg gcacagttac tgcaacagaa
     8281 gactcaatcg tagtggatgg cgaagcaact cgtgtttatg cagaacccga cgcaagcaaa
     8341 atcccttggg caaaagaaaa cggtgtagat atcgtactag aatgtactgg tttttataca
     8401 tctgaagaaa aagcaaaagc gcatttagat gctggtgtca aacgagtagt tatttctgcc
     8461 ccagctggtg ctatgaaaac catcgtctat aacgtcaatg atgatacatt agatgaaaac
     8521 gacaaaatca tctcagcagg ttcatgtaca acaaactgtt tagcacctat ggcttatttc
     8581 ttaaataatg aatttgggat cgaagttggt acaatgacaa cagttcatgc ctacacttcc
     8641 acacaaatgc tcttagatgg acctgtaaaa ggcggtaatt tgcgtgctgc tcgttcagct
     8701 gctgacaata ctatcccaca ttctactggt gctgcgaaag caatcggctt agttatccct
     8761 gagctacaag gaaaattaca aggacacgca caacgtgttc cagtcgttga tggttcatta
     8821 actgaattgg tttcagtatt gaaaacaaaa gtaactgctg atcaagtaaa cgaagcaatc
     8881 aaaaaacaca cagttgacaa tccttcattc gggtatgatg accgtcaaat cgtttctagc
     8941 gatgtcattg gaacaactca aggttcgatc tttgacccaa cgcaaacaga agtaacaact
     9001 gctggtgatt tccaacttgt taaaactgtt gcatggtatg acaatgagta tggatttact
     9061 tgccaaatga ttcgcttact agaaaaattc gctaacttgt aatttgatag atcaatcgat
     9121 aaaagaagcc tttgactgga tccagtcaaa gg
//
LOCUS       DARBDV010000107         8904 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000107 DARBDV010000000
VERSION     DARBDV010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.109"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(305..661)
                     /locus_tag="SCM56_002513"
     CDS             complement(305..661)
                     /locus_tag="SCM56_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEG1111253.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(675..1085)
                     /gene="spx"
                     /locus_tag="SCM56_002514"
     CDS             complement(675..1085)
                     /gene="spx"
                     /locus_tag="SCM56_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEG1111254.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            complement(1432..3618)
                     /locus_tag="SCM56_002515"
     CDS             complement(1432..3618)
                     /locus_tag="SCM56_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEG1111255.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            3836..4519
                     /locus_tag="SCM56_002516"
     CDS             3836..4519
                     /locus_tag="SCM56_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEG1111256.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(4600..5319)
                     /locus_tag="SCM56_002517"
     CDS             complement(4600..5319)
                     /locus_tag="SCM56_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEG1111257.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(5631..5837)
                     /locus_tag="SCM56_002518"
     CDS             complement(5631..5837)
                     /locus_tag="SCM56_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111258.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            6082..6684
                     /locus_tag="SCM56_002519"
     CDS             6082..6684
                     /locus_tag="SCM56_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HEG1111259.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            6825..8105
                     /locus_tag="SCM56_002520"
     CDS             6825..8105
                     /locus_tag="SCM56_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEG1111260.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            8118..8846
                     /locus_tag="SCM56_002521"
     CDS             8118..8846
                     /locus_tag="SCM56_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1111261.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
BASE COUNT         2587 a         1667 c         1463 g         3187 t
ORIGIN      
        1 actcagttat cttatcatgt catttctaac atgtcaacaa ctattttata tgttttttaa
       61 aatactattt tattgctttg ctacttaaaa caacagaaat gaatatatca tgtaaattta
      121 cacatgtcaa tatattttta actctatatc cccacctaga tttgaatttt gaccacataa
      181 attgagcatg tctctatagt agaaaaataa agtcaaaaaa agaacacata acacgcttga
      241 tgctaagtgt tcgactgtta caactgtatg aaatgctcag tgacttgatt aaaacagtta
      301 aagtctattt atttaagata agctgttcca tcaactgctc attgtatcca ataaatagaa
      361 cttcatcgat aataatcaaa ggacgtctga ggagattggt atgttgtgtg cataaaaaaa
      421 tcagttgttt ttttcctaca caggaaaggt caatcgcaat gttgtgaaaa gcctcagacc
      481 aagtcgcaaa tagctgttct gcttgcactt cttcaaaact gcataattct tctgcttctt
      541 ctgtacttag atcgcgttct tctaagtcaa caaattgata aggcaattga tggtgttcta
      601 accattgttt cgccttcaga cttgttacac agtgcgctga tccatagact ttcacttcca
      661 cgaaggctcc ctccctatga atttaagatg ttattgtttt gtaaatagat cagatttctg
      721 gcaacacatc gaatttcacg agggacaaat gaccggatct catcttcatt aaatccgatt
      781 agtattcttt tttcatcaat caaaataggt cgtctcaata atgaaggata ttctttgaat
      841 aagacgatca tttgctttaa agttaattcg ttaaaatcta tgcccagctt ttcgtaaatt
      901 ttcgaccgag tggaaataat atcttgaact cctccttcag tcgctgccaa gatctttttg
      961 atctcacttt ctgttaacgg ttcggcaaaa atattcttct cttcaaattc aagttgattc
     1021 gcttgcaacc atgctcttgc ttttctacag gaagtacagc taggcgaagt atatactttt
     1081 atcattttct cactcatttt taattataat ccaattctac agtaatttct tttaaaatac
     1141 caattattag ctaataatat ataaaaaaaa atcaaaataa ctgattattt acgtttgaat
     1201 aatgagaatt tttctatata ttctctattt tgataatagt attctcattt tcaaaataaa
     1261 taagggaggt gtgacaaaat tcttgtcaca cctccctttt ccgaataaac tgtgttccag
     1321 aaatagcttc ttcgaaaata agctgaaatt tcacaaaaat ttgagaagca attttcgtac
     1381 attccttctt ctttcttgaa actgaccact tctgtcacac cctctcttct atcactttcc
     1441 ttttttcaca cgactcccgg tttctttgat aaatctcgat actttcaatt ttcttgaatt
     1501 tttcatttga ggtacacata gatacagacg atttcttgct ctagtcatcg caacataaaa
     1561 tagtcttcgt tcttcttcta tcgtcgtcac tggttctgct tttatttcat taaagtcgat
     1621 tactttcttt tctgtcgctc cgttataagg caaacttcct tcgttacaac caatcaaaaa
     1681 gacatttcgg taactaagac ctttactttt gtgaatgctc tggatacata gtgcatcaag
     1741 ttttccttgt ctgtaagcaa tcaagcgggt ttttaattct ttagctgtcg cttgggtttc
     1801 ttgcgataag gcttggagtc ttctgaaagt agcatgctgc tttacttctt ctcgtatcca
     1861 ttcttgatca ctctggtcga tttccttagc tatacgtggc agatgaagat agctttgaac
     1921 aagcgaatag atttcttgat gctggtctgt tttacgcaac gcttcctcaa actctcggat
     1981 ttctgcagat ttcgatttta aattggggct tatcgacaaa atataatctg caacggctat
     2041 attttgatat ttaggagaaa agtattgatc gcattcactt tttttcaggt agcagtctgc
     2101 catcagctgc cggagcaatt gctggtaggc ctgatttttc ttttgcagat tcagtgctgt
     2161 ttgccaatga acaatttttt gatagaaatg ttggaaagac ttgataccaa agataccttc
     2221 atatggacta tctgcttcca gtacaaaagg aatattcgtt ttcagcagag attggacaag
     2281 ctgctgcgtt tgtgaagctg atcggtataa tatgattgat tccttatatt tcccattttt
     2341 ttgaataaat gtctgaattt gtttgattag ccactgactt tctgctttag tatcagaaaa
     2401 caaataggct tccataacac ctgcttcttt ttgtgctgga atcatcgcct ttggtatccg
     2461 atcattttta ttatagctga tcaatctatt tgctccttgg acaatagctg cccgagaacg
     2521 ataattagtc aataagaaaa gagttttggt aggatattct ttttcaaagt tcttgatgta
     2581 tgctggctct gatcctctga atccatagat actctgatca tcatctccaa caacaatcaa
     2641 cttgatcctg ttttcctttg tcatccgttt cacgatttcc cattgaagtg gattagtatc
     2701 ttgaaattca tcaatgaata gataatcata tttttggccg atataatgct ggatctcctc
     2761 attctccaaa gcgcttttca gattttggag tacatcattg aactctagca attgctggct
     2821 tcttttttgt tcttggagtt ccgtgagcaa tccatacagt cgttctgata tcggctgtcg
     2881 tgcattctta tcaatggaat catatttttc tttaactaac tgaaccattt cttctactga
     2941 aaggttcata ttctgccaat agtctaaccg agaagcaatc gttttttctc catattgatc
     3001 aaatatataa agattatatt tacgtatcag ctgttgcaaa acagcattca ctttttgtgc
     3061 agtatccttt gaaaagtctc cttgtattcc ttgcattgct acttcaggaa attttcgaca
     3121 aatttcattg tatatcctaa aaaagatact atggaatgtt ccgatatgga cagcggtttt
     3181 tctctggctg aaattatcta ctcgtttttt tagttcattt gcagcgttcc ttgtaaaagt
     3241 gatcattagt atgcgttctt cgtctacttg tttttgcgtc atcagataaa cagcttttgc
     3301 acagattgtt tgcgttttgc cgctcccagc tcctgctatg attcgaagat gtttcttcat
     3361 atcatatgtg atagctgctt tttgttcttg attgaagcga ttataggaac gtttctccat
     3421 cattttaaag aaagagctgt ttccttcact ttcttttact tgtacttctt cctcaataag
     3481 tccgggtgct tcgccataag ccgcttcata ggtagcaatg atttcttctt gtagccgaaa
     3541 gacttgttga agctttttat acttgttctc ccagcgtttc aattcttttt gtaatcggag
     3601 aatttctttt tcattcaact gattgatttc cttcccatct ttcttctttg aatttgttta
     3661 tttcaagttg gttatttaaa gtttcgttgt ctattttttc tttatactaa cagacctttc
     3721 taaaaatgaa aatgggaagc gatatgattt tagttttctg aaaatttttc ctttttttca
     3781 atcgtttatg ctaagataag gaagacaaaa atattcataa aaggtgatga aaaaaatgca
     3841 gacttccttt cacgaggggc gtacaatagg acttttgctg acatttatcg gaggagccat
     3901 ggacagttat acttatatcc aatatggtgc ttttgcctct gctcaaactg gcaatatcat
     3961 tctatcgatc atccaagcat ttgacggaga atggctaagt gttggtaaaa aagtattgtc
     4021 tacccttttc tttttcttag ggattcttct aacaaagtat ctaattgatt attttagaaa
     4081 aaaagaaaaa catttctggc gcttgtttgt tctatattat gaagcagtga tttttttctt
     4141 tgttagtttg acacccgtcc atgagcatcc cgcattagta acgattttga ttgcttttac
     4201 tgcatcgatc cagtgggtag cttttgataa aatcaacggg cgtgcctaca ctaatttatt
     4261 tacaactgga aatttaaaag gggtagcaac gaacttatat gattattgta tttctaaaga
     4321 aaaagcagat tttgatcgat ttttccacta tctaagtgtc gttcttgcct ttatcgcagg
     4381 agctattgtc cttgttttct gtcatcatct gtttggcgcc cattcgattt tgatcgtttc
     4441 cattttattt ttgatcatgg ccctcactca gacatttcat ttatggcaat tttatcatac
     4501 gaatcggttc actatttgat ctcaatgaat caaaaaaact aagcaagatg ctgctcacag
     4561 agaaatatct gcgagtgagc aatttgctta gtttttatat tatttatttg taaagattcc
     4621 ctttgctttc gggttatttg tagtgtagcg gatcgactgg gcatctgcct cttctgtcat
     4681 tttctttgcc cgcatcttga tttcttgtac gatttgttct ccacatgccg gatcggctac
     4741 gaaatcatat ttgtccccat cgatcactat tttgggacat atatcaaact gttcatacca
     4801 ctcttcgtat cgtcgattga gttctttata gtaggaatag agttcaggat cttgttctag
     4861 ctgttcatat tcccggcctc tttttttgat tctcgctaac atagtttcaa aggatacacg
     4921 aatataaacc agtagatctg gtttcttttg tggcgaaatt tcttctaact ccttcagcat
     4981 cgtttctaaa agaccttcat actgctttac ttctacttca gaaacacgcc ctaaatctgc
     5041 attcaaatga aacagcatac tatcttcgta gatggagcgg tctagtacgt tgtcatcatg
     5101 gctgaaagca tctttgattg ccaaaaagcg tttgttcaaa aagaaaatct gtaataaaaa
     5161 agtatacttt tctggattag aataaaatag cggcaatact tcattatctt ccacgttctc
     5221 ataaaaaggt tttgtttcta aatcttgggc aagcatttca gttagtgtgc ttttccctgc
     5281 accgatcgtt cctgcaacaa tgatcacaat tatcggcatc cttcctgtaa taaaatctgt
     5341 tcttcatttt actacttcat ttatagaaat aaaagccttg actttatctg ctccttaaaa
     5401 gctacttctc taaaagtagc caaaagtaac tcctattcat gaaaataaag gctgtgacaa
     5461 aagtcttgtc gcagcccttt tttcaaataa acagtattct cctgccaaca cctcggtcac
     5521 aaggcaatat tcataaaaca tgaaaagctg ttttttaaca ttattccttg taactcagag
     5581 ctaaacggga gactcacaac ctctttatgc tctcaacatt ttatctgttt ttattcttct
     5641 attgtacttg atttgcgtac aactaactgc cagtgcagat tatgagcggt atcattgtct
     5701 tctactgcgc atttcatgac acgtatcatc acactatttt catagatttt ttctattttt
     5761 ccttttattg gtgattcaaa taaatcagat ttaaatgcat atgtttcgcc gacttcaaac
     5821 aattgttcat tttccataag aatccctcca tcacctttta taaggtcagt aattttttaa
     5881 cataaatcac tcgaaaaagc acaaacttgt cataaaaagt acttttcgta tcaaaactgt
     5941 cacttttttt ggtaaggttc ttggaaaacg aagatttttt taggagatca tgacggtttt
     6001 gatgtcaaaa ccacttttta tgaaacaaaa gacgtttttt atttttttta gcataaaatt
     6061 tgtggtggag gtacgtatga gatgaaagat aaagttgtga acaaattatt agaagcctta
     6121 ttcagcggag acaaatcgga agaagatatg gtgtatgtcc aagtaaaatt tgccttagaa
     6181 gttttattga ataatcttgg aaaactagcg gtcgttctag tcttttctct ggtgacaggc
     6241 tcatgggcgg aaacaggaat cacctttctt tcttatatct gcatccgcag atatgcttat
     6301 ggattgcatt ccgactctga attcgtttgt ctgctgtgga cactacttta tttatggggc
     6361 gttcccttgg tgatgaaaca tctgcaattg actatctcct tcccaatgat ggtctttttg
     6421 ttaatcagct gctttttgtt actgcttcgc tatggatcaa gaggtacagc aatcaatccc
     6481 attgaacccg agaaacgtcc cccattatta aagaaggcga tcagcatgtt tctaattttt
     6541 tctctcatta ctttattttt ctcagcttct tacttttcta cctatttgct acttgggatt
     6601 gttttagaaa tagctacgtt gcttcctatt acaaactact tgatgaattt tggaggaatc
     6661 tttcatgaaa aaaacaatca ttaaacaatt aactaactta tctgactttt ttgtatctgg
     6721 ctgctgcttt ggtatgagtt ctgaattcaa aatccctgaa aaattaaaaa aataatacgc
     6781 taattgtgct ataatttggt aaaaatcaat gggagctgta ctttatgttt ttatggtcca
     6841 tagcgttgca atatttgatt tacacaattt gtatcttatt gattgaccat cacatccgat
     6901 acaaatatct gtttgcggga gtcattctga tttcttgtat tttgattgga tgggtctatt
     6961 cgattttagg gatttttact gctctaattg ctataggaat gctgatgctt acttttttct
     7021 tcttttcgac ttcgcgtcaa caattggttt acgcgcttcc ttttgggtta ttgacgtcgc
     7081 ttttaggaga tcacttaaca agcatcgacg atttttttgt tttgaaatct tcggttattg
     7141 aaccaggaga ttcattgcag tattttcaca ttgtcttatc cgctttttta tctgctttat
     7201 tagctttttg tttaaaaaaa tcgttagaga cttggtttca cactgtggac cgccgtatga
     7261 tcggtacgat cgggacattg atattattta catactatat cattattttt tatacccgtt
     7321 tttctggtga aacacctgat gtcttagctt tgaatacgag ttttttcatt ctatatctgt
     7381 gtgttggcct gatcatcttg gtttattatt ggaaaatctc taataagaaa ttagctgatc
     7441 agttattaga gcaacaagtc aggatgcaac aggattatat caaagatcta gaaaaaaact
     7501 atcaagactt gagagaattc aaacatgatt accaaaatct gcttttttct cttcacagct
     7561 atatttcaga aggcgactta gaaggattac ggtcttatta tgatcaaacg atattaccta
     7621 cacgccagat gatgaacctt tttccggcca atctaagtat gttggataat atgaaagttc
     7681 ctgagatccg tagtttactg tcattaaaac tcatgatggc acaggaaaaa gggttgatcg
     7741 tccagcttca ttttcctgag ccaatcgaac tggatacgaa acatacggta aaccttgtac
     7801 gtatgcttgg tattatcttg gacaatgcga ttgaaggagc tagtgctgca aagaagaaaa
     7861 aaatcgaatt gaccatactc aagaagaatc attccgtgat gattcgtgtg atcaatacaa
     7921 caacaaatac attaccgtta aaccagttga aacaaaaagg gttctcaaca aaaacaaatc
     7981 caaaaaacga aggccttggc ttgtttatct tggatgaatt gacaaagacc aatccttatc
     8041 ttttccttga aacatcaatt gaaaaccagc gtttttctca aacgatttat atacaaataa
     8101 aataaaggag taatgatatg attcccattt atatttgtga agacgatcca aaacaattag
     8161 aagttttatc aacggtgatc aaaaatagaa tcatgattga aaacttagac agttatgtcc
     8221 atatttctac ctctgatccg gaagaacttc tcaatgcagc caagatcaga acttcttctt
     8281 ttggtattta ctttttagat atcgaactag aagacagtga aatggatggt gtagcgatcg
     8341 gtaaattgat ccgtgaatta gatcctttag gaaaaataat ctatgtgact tcccatacgg
     8401 aaatgtcgat gaaaatctta aaaagcaaca tcgaaccgac tgattatatc gtcaaagaag
     8461 atctttatga tttaaaagaa aatgtcgagc atatcctgag caaaattttc gaagattttc
     8521 aaatgtccca aatccaaaaa gatatcttca aaatagagtt taatgatgag atcaaatttt
     8581 taccgatcaa agacatccaa tatttttcta cttcgctagg agcgccacat aaactggaag
     8641 tccatttgac tcatgcccaa ttacaattct atgagaagat caaagaaatc gagaaaatgc
     8701 atcggaattt tatccgttgt cataaatcct atgttatcaa tacacaaaat gtgcggtcga
     8761 ttaataaaaa aaccagagaa gtcacgttgg caaacggcga gaccatccca gcctctatcc
     8821 gtggattaaa gaaattgatt tcataaaaag aaccgaatag tttggaccga ctaaatgacc
     8881 agccgatcca aacccttcgg ttct
//
LOCUS       DARBDV010000108         8800 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000108 DARBDV010000000
VERSION     DARBDV010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.110"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..778
                     /gene="menA"
                     /locus_tag="SCM56_002522"
     CDS             <1..778
                     /gene="menA"
                     /locus_tag="SCM56_002522"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEG1111262.1"
                     /translation="NYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVI
                     GVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYV
                     NTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDI
                     ENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHL
                     DQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1592..2938)
                     /gene="gor"
                     /locus_tag="SCM56_002523"
     CDS             complement(1592..2938)
                     /gene="gor"
                     /locus_tag="SCM56_002523"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEG1111263.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(3072..4325)
                     /locus_tag="SCM56_002524"
     CDS             complement(3072..4325)
                     /locus_tag="SCM56_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEG1111264.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(4331..5254)
                     /locus_tag="SCM56_002525"
     CDS             complement(4331..5254)
                     /locus_tag="SCM56_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEG1111265.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(5475..7967)
                     /locus_tag="SCM56_002526"
     CDS             complement(5475..7967)
                     /locus_tag="SCM56_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEG1111266.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(7974..8441)
                     /locus_tag="SCM56_002527"
     CDS             complement(7974..8441)
                     /locus_tag="SCM56_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEG1111267.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(8577..8650)
                     /locus_tag="SCM56_002528"
     tRNA            complement(8577..8650)
                     /locus_tag="SCM56_002528"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8614..8616),aa:Arg,seq:acg)
     gene            complement(8708..8781)
                     /locus_tag="SCM56_002529"
     tRNA            complement(8708..8781)
                     /locus_tag="SCM56_002529"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8745..8747),aa:Pro,seq:tgg)
BASE COUNT         2441 a         1867 c         1461 g         3031 t
ORIGIN      
        1 taattacatg gattatgaaa aggctcattc tgaagtatat aagtatgaag aaaatgtcat
       61 tggccgcaga aatctatctc ctgtattgat tcgaaacatg attttagcta tgattgcatt
      121 tgtttttgtt atcggcgtta ttctaacgat caatacgggt tggcttttac ttttgatggg
      181 atttatctgt tgttttattg gagtattcta tacattcgga ccgattcctt tatctcgaat
      241 gcctttaggg gaaattttca gtggggtgac tatgggcctt ggaatttttg ccatggtcgt
      301 ttatgtcaat acattcggtt cagatgtcta tgatctgaca gtcaactttt catctggaca
      361 atttgatttg gtaggaaatc tttgggctat agcagcaatc gtattagctt ccttaccctt
      421 ggtgtttacg attgccaata tcatgttggc taataatttg cgggatcttg accgtgatat
      481 tgaaaatcat cgctatacac tagtttttta tatcggacgt cctgcgggaa cagtcttatt
      541 ccagctattg atgtatgctt gctatttcgt gattttaatg gggttgttga gcggtatcta
      601 tgaatggccg atcttggtca ccttcttgac cttgccaatt gtgaagaagc atttggatca
      661 attcaagcaa acgttacccc agcctatcag tttcagctat tccatcaaaa atatggtctt
      721 attcaatgcc agttatgtat tggggctatt actaacaatc attatgcaag ccatttaaca
      781 gcatgaagag gttgtgaaaa aactgccctg catcaagtaa taagaacagc caaataaaaa
      841 tagctcctta tatttttcgt ttggttgggc ttattaccgc agatgcaagc ttttgaacac
      901 cgtttatcag gaggttgtga aaaagtcgct tagtttcgag tattaagaaa aattttgtga
      961 aaatggcttt ccacattttt gcataaattt ggcttaatac cgagaaactg gcttttgaac
     1021 accgtttatc agaggttgta aaatcagtgt tttgtcctga gtattagaga aacaaatgga
     1081 aaaatagttc ctcatatttt gccgtttttt gagctaatac cgaaggacag ctgattgaac
     1141 actatttgtt cggaaaaagt gactaactat gacacaattt ttttcacagt cccttttttc
     1201 acttgatttg attcgtacca gcgaacttga cgagaacttt cttggtttgg taactatatg
     1261 gtattctttt aacatcctcg tttgcttctt ctaatttatt tatacaaagt cacacttttt
     1321 taaacatctg ttcttcgata catttctatt tcttttaaac atcgatacca atattttgaa
     1381 cttaagagtg cataccaaat ggattcattt actcttcgtc agttacgaag ataatcaaaa
     1441 tctccaactt ccctacttat ttcgttttga gaattatcga agcaatctta cataaataac
     1501 taaaaaacaa aaaaccagaa aatcagtata ctgattttct ggttttacat ggtttgttca
     1561 ataaaaagct gttggaactg ttaatattaa attatcgcat cgttacgaat tcttccgctc
     1621 ctgttggatg gatggctact gtattgtcaa agtcttcttt agttgcaccc atcttgatag
     1681 ccacggcaaa accttgcagc atctcatcga caccaacacc aatagcatgt aagccgacga
     1741 ttctttcttc ttttccgaca cagatcaatt tcatttcgca tttttggcga taatcattta
     1801 atgcaaagta cattggggta aagcgtgaac gataaacttt caattcattt tcgccatatt
     1861 tttccaatgc ttcttcttct gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga
     1921 ctgtcggaac aagtttatag tcaagataag aatctttctt tccattaaat aaacgttctg
     1981 ataaacgtct accagctgcg attgcaacag gagtcagatc taatttaccg atcacatcac
     2041 caacagcaaa aatatgttca tccgttgtat tttggtattt atctactttg ataaaccctt
     2101 ttttgtccaa ttccacattt gtattttcca gacctaattt ttcggtattc ggtacacgtc
     2161 caccggcaaa aagtacacaa tcaccagtta aggtttcacc attttcaaat agcaccgtat
     2221 attcgtcatt gtttttagta atttctttag caatatgatg tgcgtaagtt tttattccag
     2281 cttcttggta catttggatc aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc
     2341 gttcttttcg atatgcccac atgacttcac tacctaaacc acgaaataca cctgaaagtt
     2401 cagccgcaat gtatccagca cctaaaacga ttgctctctt aggtaactct gttaattcaa
     2461 aaaatccatt tgaatcaatt gcgtattctc caccaggaat ggtcatttta cttgcacggc
     2521 ctccagtagc gatcaagatt ttaggagcac gataggtttc accgtttact tcgatcgttt
     2581 gactatcgat aaaagtagcg taacctttta tagcctcgac atgattgcta tctaatcctc
     2641 tttgataagc accgtgtaga aaatcaatat atttttctcg tctttctact agaccggcga
     2701 aatcaacatt ttttaaatct gcttggatac cgtaacttga ggcatctcgt tcaattgttt
     2761 ccaaaatcgt acttgcttgc cacataactt ttttaggtac acaaccgacg ttgacacatg
     2821 ttccgcccaa ttcattcgct tcaatcagca aaacttttgc gccatgcatc cctgctctgt
     2881 ttgctgaagc aataccgcca gagccgccac caattacgat gtaatcatat atttgcattt
     2941 tgttcctcct tattgaagag atcaatcaaa acctttattt aacgatcctt tttctacaga
     3001 aaacaagtgt aacacttttc ttgaattttg tatgcgaaga tgcttaaaac agggatatat
     3061 cagctttttc cttatttacg ttttcggatc atatctccag gtttcaccgg ctgtgcgact
     3121 ggcatagtta aaatcatcat tggatttggt gctcggtcaa tggattcgcc ttcttcatta
     3181 tgcataactt caacggtttg actgaaatga ctgaatcctg gaccataaaa ttcgatttca
     3241 tctcctacag aaaacaagtt tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa
     3301 gataatactt caccgacaaa tttatatact gggattttac gacgttcacc gaataattgt
     3361 tcgttttcag ttggtgtatt gtagtaaaaa cctgtagcta attctcgttg tgctactttc
     3421 cataactcgt cgatccattc ttgcttgcag acatagtttt caggatcttc catataactg
     3481 tctacagccg ctttatagac atttgacacc gtagatacat aatggatcga tttcattctt
     3541 ccttcgatct tgaagctgtc tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt
     3601 gacatatcca ctgcactcat cgagaattct tcttccactt cgcctgtatc agttaaactg
     3661 tttcgttctt gaccaaaagg catatcgaac aatccatact tccagcgaca agattgagaa
     3721 catccgccac ggtttgcatc ccgcatcgac atatggttgg acaatgtgca tcgaccagaa
     3781 taagagatac acatggcacc atggataaat gcttcgattt cggtatccgt atttttacga
     3841 atctctgcca cttcctccat tgaaacttca cgagctaaaa cgacacgttc caacccttct
     3901 tctttccaga attccaatgt ctcaaaatta gttgctgaag cttgagtgga taaatggatc
     3961 ggcagacctg gggcttctgt cgcacaaatt tcgatcaaag ctggatcaga aacaatcact
     4021 gcagaaatac cgacatcacg caaactgcgg aaaaattccc ctgctccatc ttcatttcct
     4081 tcatgggtga ccatattagc agcaacgtat accttcgcat catgttcttt tgcaaaagca
     4141 actccttcag ccatttcttc ataagtaaaa ttacctgcac ggcttcttaa accatatgca
     4201 ttcccgccaa tatatactgc atctgctcca taatggatcg cggtttttaa cttttccagt
     4261 gttcctgctg gcgctaggat ttctggtcgt ttcaattttc tttttactgt catttctttc
     4321 atcatactcc ttatttgata gcagaaggat caatataaaa gaatcctgta tctaattcac
     4381 ggttttcagg atgcaattta ctgatttcgc tagtcatttt ttccgctttt tcttgatccc
     4441 agctaccttc taaaatttgt ttttttgctt cggcgaatag tcgagtgatg tttacgaatt
     4501 tttcgcctgg agtaaataat ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt
     4561 cattcaattc attcgagaga ttcagatcat tgctggcaaa aatatgtgtg ccatgactat
     4621 cttcataaat agaataatgt gtctcttctt ttttaggttc cgagataaaa agacctcttt
     4681 cacgatcttt ttgttcgtct tgtttcgtat agttatagta attctgtagc agtggacgtt
     4741 tggattgatg gatgcatgta gcaccataca caagggtttc tacaggaata tcgagttttt
     4801 cttccatcgc cttcatctct tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc
     4861 cttttttcgc ccagaaattg atttgacgtg cgctcgttac taatgtttca gcatcataaa
     4921 tatacggtat acgcaattca ggattttttt tcatcgtata aataatacct gtatcaccaa
     4981 ctgtgatcac atctacgttt atttctttta aaaaagccaa atattcaggt acatttttca
     5041 ttttttcagg atgaatgatc ccatttacag caatattcgc ttttttacca tacttgtgtg
     5101 ctaattctac caattgtcgc tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa
     5161 attgctcttc accgaaatat agtgtatctg cacctgcagc aagcaactgt tctgcttgtt
     5221 caatagtttc tactgtggtt atgagttcga tcataatttt ctcctcctac aacttcataa
     5281 tagtagcata gctctaaaaa ctttccaata cttcttctat ttaaagtcgg atttttgaaa
     5341 attatttcac aaaaaatcaa aataaagttc gatatattct gtccaaatat aaaaagagat
     5401 tgtgacaaaa atcttgtcac aacctcttaa tcaagtctta ctattataaa aattcagcta
     5461 cacaggccaa gtaactatag ggaacctact gtttcctttt taggtccgcg aacattcaat
     5521 gtgatcttac cttttgaagc acctactgtt acgtggtcac ctaaacgaat ttctccgcca
     5581 agcaaagcct cgctcaatcg atcttcaatt tctttttgaa gtgcgcgacg gattggacgt
     5641 gcaccatatt caggatcaaa tccagctttc ccgatcacat cgattgcaga tggtgtgatt
     5701 ttcacttgaa tatcttgttc tttcaatcgt ttgataatcg ctttgctcat gatttggacg
     5761 attgtatgga tttcatcttt actcaatgaa tggaagacga cagtctcatc gattcgattc
     5821 aagaattcag gtcggaaagc tttctttaac tcttctaaga tacgtttttg catggctttg
     5881 tgatctttag taacatcttg gacattgaat ccgacatttt tttcttctcg aatttgtgtc
     5941 gctccgatat ttgaagtcat aatcatgatt gtatttctaa agtccacttt tctaccttta
     6001 gaatctgtta aatgaccatc atccaatact tgcagaagta aattgaaaac gtctggatgt
     6061 gctttctcca cttcatctaa gagaatcact gaatatggtt tttgacgaat tttttctgtc
     6121 aactgtccac cttcatcata ccctacgtat cctggaggag acccgattaa tcggcttgta
     6181 ctgtattttt ccataaattc tgacatatca acacggatca gggcatcttc acttccaaac
     6241 atgacttctg aaagtgcttt ggccaattct gtttttccga caccagttgg tccaaggaac
     6301 atgaatgaac caattggacg atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca
     6361 cgtgaaactg ctttgacagc ctcatcttga ccaacgacac gttcatgcag aagtccttcc
     6421 agttctaaca agcgttcgct ttcttttttc tccaactgct gtagagggac accagtccac
     6481 tccgatacaa ctgtagcaac atcttcttct gttacacgat cagaataacc ttgctcctgt
     6541 tttgcttcaa ttgcagaaac tttttgcagc tttttacgta gtgctttttc ttgtctgcgt
     6601 aattgagcag cattttcaaa gtcttgtttt tggatcgctg cttccttttc ttgtactagt
     6661 tcatcgattt ctagtttgat cacagttgat tttgttaagt gatcggtttg atccaaacgg
     6721 actttcgctg ctgattcatc gatcaaatca attgctttat cgggtagttg acgagaagtg
     6781 atataacgaa cagaaagatt gactgctgct cgtacagctt catctgtgat ctccactcca
     6841 tggtgctctt catatcttgt acgtaatcct tgaagaatca cttctgcttc ttcaggagtt
     6901 ggctcatcta cttgaatgcg agcaaaacgt cgctctaatg cagaatcttt ttcgatatat
     6961 ttttgatatt catctagggt cgttgctccg atcgtttgta attctcctcg agctaacgcc
     7021 ggtttcaaga tattcgaagc atcaattgct ccttctgctc cacctgcacc aattaaggta
     7081 tgcaattcat caatgaataa gattacttga ccatcattat agatttcgtc gatgaccttt
     7141 ttcattcgat cttcaaattc accgcgatat ttcgtaccag caaccaaaga tcccatatca
     7201 agcatcatca ggcgcttttc ttgcatgtct tctggcactt cacctaaaat gattttttga
     7261 gctaaccctt cagcaatggc tgttttgcct acaccaggtt cacccaccag tacagggtta
     7321 ttctttgtcc gtcggctaag aatctggata agccgtttca cttctctcga acgaccaaca
     7381 actgggtcca gcttgttttc ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc
     7441 gtaggtgttc cttgcggtgt ttgccgttta tcttgtacat ttacatttct acgacgattc
     7501 attccttttc cagcagaatc accagctagc cccatttttt gtttcagaac ttgacgggct
     7561 tttgaaaggc tgatacctaa gttgatcatg attcgagtag ccatgatact ttcatctttt
     7621 aataagctca tcaaaatatg ttctgttcca actttttgag aaccagtttt cttcacttcg
     7681 ctgctagcta aagcaaaaac ttgtttcatt cttggtgaat atggaagaaa gactccttta
     7741 gggtaagaac ggataccacc ataaccaatc agatgttcaa tttcttcata aatttccgtt
     7801 tcattcaaat ccatttcacg taatactttt ccagcaattc cattttgctc gaccactaag
     7861 gctagtaaaa catgttctga actaactgat tgatgtttca accgttttgc ttcttcttgt
     7921 gcaatttcta atactgcttg ggctctttca gtaaaaagtt cttccatcta atctcacttc
     7981 ccatcttcat aacttaaacg ttccagaaat gctcggagaa tccgagcacg tgtatgatct
     8041 tcatattcat tgaaattcaa tgtgtttttt gcaattgcac tcaacatcaa gtttccttct
     8101 ttttttgtaa ttatttggtc ttcataaagt ttttgaataa tagcaaaagc atttttctca
     8161 ctgattgtat catcaaataa ctgatttatc tgctcaagta attgtttttt atctgaaatc
     8221 cttacttttg caattcgtat ataaccacca ccaccacgtt tgctttctac tagataacca
     8281 cgttggatgg taaaacgggt attaatcaca tagttgattt gtgaaggaac acagttaaag
     8341 aggtctgcca tttcaatcct tcgaatctcg atcatttcac tattttccag aattttcttt
     8401 atatatgatt caattaaatc cgaggtattc tgatgtgcca tcttattcat tccctttgac
     8461 taatattgac cttaattata acgaatttga taatagaaag aaagaagaac gctttttgtt
     8521 aaataaatcc tttagaatca acaaaaaaaa atacggaata tactattccg tatcgacgcg
     8581 cccaagagga gtcgaacccc taaccttttg atccgtagtc aaacactcta tccagttgag
     8641 ctatgggcgc atattcaatt taaaaaaatt ataaatcatt tgaaaacaaa aagcaagata
     8701 aaaaaaatcg ggaagacagg attcgaacct gcgacccctt ggtcccaaac caagtgctct
     8761 accaagctga gctacttccc gtttaaaaaa taaaaaagac
//
LOCUS       DARBDV010000109         8675 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000109 DARBDV010000000
VERSION     DARBDV010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.111"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1101
                     /locus_tag="SCM56_002530"
     CDS             <1..1101
                     /locus_tag="SCM56_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEG1111268.1"
                     /translation="KKETIVRFEQYNHMPLVTRLKKQRYTCKNCRTHWTAQSYFVRLS
                     HSIADHVRYKIASLLTEKVSLSFIAKSCQVSLTTVTRTLKEFKSYLPKPSKKILPRVL
                     MVDEFRSHASIEDKMSFICADGETGNLIAVLPTRKLPRLTSYFLGCTNPEEVEFLVTD
                     MNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQALNELRIREMNELRKAGQKSQAEKLKK
                     NWRFLLKNRASINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSAPLKEAYPFFHELV
                     EAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITSAMIYPYSNGKIEAKNT
                     HIKTMKRVSYGFKSFENMRIRIFLINQLINVR"
     gene            complement(1280..1609)
                     /locus_tag="SCM56_002531"
     CDS             complement(1280..1609)
                     /locus_tag="SCM56_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEG1111269.1"
                     /translation="MNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSKYH
                     TKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQRL
                     EERMEEL"
     gene            complement(1599..1871)
                     /locus_tag="SCM56_002532"
     CDS             complement(1599..1871)
                     /locus_tag="SCM56_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEG1111270.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2322..3125)
                     /locus_tag="SCM56_002533"
     CDS             complement(2322..3125)
                     /locus_tag="SCM56_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEG1111271.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3103..4227)
                     /locus_tag="SCM56_002534"
     CDS             complement(3103..4227)
                     /locus_tag="SCM56_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1111272.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4243..5058)
                     /locus_tag="SCM56_002535"
     CDS             complement(4243..5058)
                     /locus_tag="SCM56_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1111273.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5048..5920)
                     /locus_tag="SCM56_002536"
     CDS             complement(5048..5920)
                     /locus_tag="SCM56_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1111274.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6005..7294)
                     /locus_tag="SCM56_002537"
     CDS             complement(6005..7294)
                     /locus_tag="SCM56_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1111275.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7522..8166)
                     /locus_tag="SCM56_002538"
                     /pseudo
     CDS             complement(7522..8166)
                     /locus_tag="SCM56_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
BASE COUNT         2691 a         1850 c         1371 g         2763 t
ORIGIN      
        1 aagaaggaaa cgattgtccg ttttgaacaa tacaatcata tgcctttggt tacgcgccta
       61 aaaaagcaac gctatacctg taaaaactgt cgtacccatt ggacggctca aagttatttt
      121 gtccgactca gccattcaat cgcagatcat gttcgatata aaattgcttc tttactgact
      181 gaaaaagtat ctttgtcttt tattgcgaaa agttgtcagg tatctttgac taccgttact
      241 cgtacattga aagagtttaa aagctattta ccaaagcctt ctaagaagat tctccccaga
      301 gtattgatgg ttgatgaatt tcgttcgcac gcttccatag aagataaaat gagctttatt
      361 tgcgcagatg gtgaaacagg aaacttaatt gctgtcttgc ctacgcgtaa attacctcga
      421 ttaacaagct atttcttagg ctgcaccaat ccagaagaag tagaattctt agtcacagac
      481 atgaacgccg cttacttcca gcttaccaaa cgtgttctgc caaatgcgaa agtagtgatt
      541 gatcggtttc atattgtcaa acacatgaat caggcactca atgagttgcg tatccgtgaa
      601 atgaatgagc ttcgtaaagc aggacagaaa agtcaggcag aaaaactgaa aaagaactgg
      661 cgctttctgc taaaaaatcg tgcaagcatc aaccattatg agtacaaaac atggaaaagt
      721 ttccgagcac caaaataccc atttctcact gaagcaatga tgattgatcg attgcttgag
      781 ttttctgcgc ctctaaagga ggcgtatccc ttttttcatg aattagtcga agcctttcga
      841 gacaaagacc ctgacttatt tttctcctta ttggcagaac ttcccgaaac gttagatgac
      901 ggctttcggg agaagcttca aaaccttctg acctatgaag aaggtatcac cagcgcaatg
      961 atctatcctt attccaatgg aaaaattgaa gcgaagaata cccacataaa gacaatgaaa
     1021 cgagtatcct acggatttaa atcattcgag aacatgagaa ttagaatctt tttgatcaat
     1081 caattaatca atgtaagata acaaaaaatc tgaaccagaa tgtggttcat tctgacccgg
     1141 atttgatttt gcacaactca tcagtcctta ttgacaaaga gccaaaaatc tgagccagaa
     1201 tgaggttcat tctgacccag atttgatttt gtacaaccca tcagtcctta ttgacaaaga
     1261 gcccttcttt ttagtattat tataattctt ccatgcgttc ttccagtcgc tgtaaatcgc
     1321 ttggacttaa ttccccaatt tttttccatt tgaccttttt gttgtttttg ggtaattcgt
     1381 ttagagttcc tacgtcaatc caagattctt tttttagtcc tgcctgtttc caatccctta
     1441 tttcaacgta tttcagcttt attttatcgg atttagtatg atattttgaa gtaattgaca
     1501 gaaacgaaat tgtgtccagt ccatttttta gaatatagac aggccggcgt ttaccgccct
     1561 ctgcagctgt gaaagcaacg tagacggttg ctatatcatt agtattcatc ttcattgaac
     1621 cattcttcaa ttttttcgtc agtgtctagt ttttggacag gcaatttttc cgctaatctt
     1681 tgaatattat ggattcgttt acttttttcc gacaattcaa tagaaaatgg gatttttcct
     1741 tgctcaacaa tttgcttata aaaaacggtg acggctgttt tggggctcat gcctagttca
     1801 tcaataatgc tttctgcttc atttttcaat ttttcgtcca tagaaatttg taacttagcc
     1861 attttctcca cctctttctg acaaacacat tatatcacgg aaagagttca aatggaatta
     1921 atacaactac ttttgaactc cttgaaatgc agtgtattta tctaaatata aggaaaggat
     1981 atagtgctaa atggccttga tagatagaaa agtttcaaaa tccgccacag cgataattga
     2041 agcgaacact caacgacaca cagaacagga aaagactcct aaaagaacca aattaactag
     2101 gacgtgtctg aaaactccac aaagatgtgg tatgctctgg taaaaaacga atggcacaac
     2161 cacgaagacc gatatggttc ttggaaaact ttctccaccc tttttgtaat tggagggata
     2221 gtggtttatt gatagcgatt ttccaagttc ttcaagtgga accagacatt gagcaccacg
     2281 gctaaaaagt gaatgaagtc tttattttta atcaataatt tttaatgatt accagtaatt
     2341 ggttttttca tgcgccctat ttctatttga tgtggagtta aataatgctg ataagtaaaa
     2401 atttttccgt caattaaact gccactagtg aatcctgttc tattggtaaa aagagaatag
     2461 tctgggtatt catctacacc aaatgataaa gattcagcca taaactgttt tttaccagca
     2521 aattcagcac tgctaccttg atggatatgt cctacaaaaa tacctaaaat atcactttcg
     2581 gcaatcaatt cagcaaaacc ttccggaact tcagttacaa tagatggatc ttcccacgac
     2641 aatggatgat gaaccaataa aatcgttccc ttttcagcag gtgtacccaa ccactcttgt
     2701 aattgaatca tctgtcctat cgatattttt ccttcgtgct gggtatcttg agcagaatcc
     2761 aaggaaataa ttcgtaaccc attttgattc gtacaatgga tatactctcc cgtttcattg
     2821 ctttcaacac tcattccttc aaagaaaaca ctgcgacgat catgattccc acgacaaaaa
     2881 tagtaaggtg tggttgggaa atattgatcc aacattttcc gtaagacttg ataatcagaa
     2941 atcccccctt catgtactaa atcacctgtt aatagaataa aatcgtacac tgttaacgag
     3001 agttcttgta acaatttttc caatgccaac tgtggatttt taattgttgg aaatagttca
     3061 gcagtaaaca agttagtttg atagttttct ctaaaatgga tatcagagag atgcaaaaag
     3121 cgcatggcta ttccctcttt cttcaataaa ataatcagga tatcctaaat tttcttgcgt
     3181 ctgataatca aaaaaatgca ttctttcagg ttctatgcgg agattgactg catcaccaat
     3241 ctgccaagta ggtttttcat taaggacaat catttctata ccatcgactt taacggtgta
     3301 cgcataactt tgacctaata gctcacgata cttcacttga ccgtgtaaat cgcttgtttc
     3361 ttttgaaaag gttacgtgtt ctggtcttat gcctaaatac aatgagccac taacctgaag
     3421 aagttgtttt tgccaagctg gattcaatgt taaaaattga ctggctacct ttaatgttcc
     3481 ttgatcataa gtaactggca taatattcat gcctggcgaa ccaataaaat ttgcagtaaa
     3541 agtattttta ggtttcgtat aaacattttt cggcgtatca atcatctgaa ttctaccttc
     3601 attcaataaa acaatccggt cagctaacgt catcgcttca atttgatcat gagtgacgta
     3661 gacaaaagtt tgtttataac gttgatgaat ttttaccaat tcttttcgtg catccaatcg
     3721 taaacgcaca tccaaatttg ataacggttc atctaataaa aaataattac tccgtttaac
     3781 caccgcacga gccaatgcta cacgctgccg ttgccctcct gacaaatctt taggaagtcg
     3841 atcttggtat ggcgttaaac ctaacatctt caaagcttct tccaaacggt gctgaatgtc
     3901 agctgtctta attttattcg cccttaatgc aaaaataata ttctccttta cagtcatgtg
     3961 aggatataaa gcataatttt gaaaaaccat tgcgatctct cgttctccac aaggtagctt
     4021 atttgctagt tgtgtaccta aaaaaagctc tccactcgta attttttcca aaccagcaat
     4081 cattctcagc aaagtagatt ttcctgaacc agatggtccc agtaacacaa ttcgttctcc
     4141 agcggccaca gctaaatcaa tttcttttaa aatggtatgt tctccatatt tttttccgat
     4201 atttcttaat tcaagcgttt ctcccatgat ttgtcctcca atttatttaa tacctgcaga
     4261 aacaaaagtc ccgataatat atttttgaaa aattgcatac aagactaacg gtacaaacat
     4321 tgtaattaaa ctaatcgcca ttgctacggt aaaatttgtc ccaccttcag aactaatata
     4381 ggtctgcatc gccaacggca aagtataatt atctactgtc cgtaaaatta ataccggcca
     4441 aacatactca ttccaagagg taataaaaaa ccaaataccg gtagaagtaa tcgcatgtct
     4501 agtaagagga aaaacaattt tactcatgac ggttgtttgt ttgatgttat ccaatgccgc
     4561 cacatctaga aggctcactg gaatttcccg cattgtttgc gtcaataaaa aaatgcccat
     4621 cgcatcagca aattgtggta ttatcacacc ccaagtagta tcaattaaat taagttttgc
     4681 aatcaacaaa taattaggaa tcatcgcaac agtaaaagga atgaacatcg taccaatgat
     4741 gataaagtac cagattttct tccctttgat tcggcaataa gtaaaactat atgccgcaaa
     4801 aaaagctacg cctaacttaa caaccgttat aattgtagca ataaaaaaag tatttctcgt
     4861 aattttgata attggaagat tttcgaacaa atagcaaaaa ttttccaagg taaacggctg
     4921 tggtatcaaa ctagtaatcg tctgataggc atctttcaat ggcttaaaag aattggccat
     4981 aataaacaat aatggataga taataatgat tatgatgaac ataaataaga gatgtggcaa
     5041 ccagcgttta attttcatag taaaccccct tttccacaaa tttaaatatt acccctaaga
     5101 aaacagcaaa tattaataaa gttaaaactg aaattgctga agattctccg actttataca
     5161 tttcaaaagc cttttgataa gcataataga tcacgtttgc actaccatta ttcggtccac
     5221 cacgagtgat gatcttaatc ggagtaaaca catactgcag accttgaaca atggtcatca
     5281 ccaataaata tagtcctgtc gaactactca taggcaacac aattgtaaaa aaaatgcgcc
     5341 agatgggtac acgatccaac ttagctgctt cgatcatctc ttgatcaatt cccacaattg
     5401 ctgcaaaaat gacaatgaaa ttataaccaa aaactttcca agcagtaata cctgcaatca
     5461 cagcaattac ccaaccttgt aaattcatcc acataggcag tcttaaacca aaatttccca
     5521 acaagacagc aattggtccc gataccggat ttaatatcca tgtaaaaacc atccccgcca
     5581 ctaccaaaga aatcacagct ggaataaaaa ataatggttt atacaaattc tgcccttttt
     5641 ctataactat tcctaataaa aaggatgtaa tatatgggaa cacacagctc agcaagacaa
     5701 atagaacggt ataggacaaa gtattcttca acactttatg aaagactgga tcagtcagca
     5761 cctttacata gttggcaaag ccaacaaatt ctttagtggg ggccaccata ttccagtcaa
     5821 aaaaactcaa atagatcgta taaatcaatg gataaaaaac aaacacgccc aacaaaataa
     5881 ttgctggcgc cagatacaaa taacctacat agcgcttcat ttccgacagc ctcctcatcc
     5941 ttatgtcaaa agcagacgac cttgtttttc ctttcttaca ggcgctgtcg tctgcttgct
     6001 ttgcttattt gagtaactca ttaatttcct tttgagtagc tttcatcgtt tcattgacgg
     6061 aattacctga taaaatttta tcccgcatat ccaacaattt ttgttcagct tctaatccag
     6121 catctccagg aaatgaactc caactgacca tcttatccat ctgatcaatt gctggtatca
     6181 tcatctgatt ttcagctaag aaagctttta atccattttg ggcgtctgca acatctttac
     6241 gtggtggtaa ataaccagta ccttctgtcc atttagctaa agattcaaca ctataaagat
     6301 attttaagaa atcccaagtt gcttcctttt tcttatccgt ttgtgatgta acagctaaca
     6361 ttgcgccacc agcaggtagt ttaacttttt tcccttccca acttggtgac tcaactgctt
     6421 tagcagtaaa agtactattt ttttgaatgt tgtccctttg agcaatagtg gtatagacca
     6481 tcgcgacgtt accatcaata aagctttgca caccttgttc ccacgtagtg tgtaatgccg
     6541 tctggtcttt taccatcctt tgatataatt cataagcagc gataccatct ttagaagcaa
     6601 aggtagcttt gccatctttt attacacttg ccccattact ttctaaaatt gcttgttgtg
     6661 cccaattatc agcaggttct tgaatataca cgccatactt tccagttttt tcttttatta
     6721 tcttagaaaa agtttgaact tcttcccaag tttttggacc attttcatcc aagcccgctg
     6781 cttttaatat atcttgattt aaatataaaa ccggattact aatggagtaa ggaataccca
     6841 cttgtttacc atctttttca gacagtgcca tgatattgtc taaaaaatga tctttaaaat
     6901 aatcagcatt atccggatcc acttcttcta ctaaagattg aggattagta tatttgaaat
     6961 tttctgcaaa ataatcttta aaagcccatc ccacttgtac tacgtccggt atttctttgg
     7021 aggcaacaga agcttgtaaa ttttgcatca agcctttata catatcagga ttatattttg
     7081 ctacaacctt cactttgtct tgagattgat tgtatttatc tactaattca ttcactgttt
     7141 ttccacctag ggtatccgca ttaacatgcc aatactctat cgttacacga ccctccgatt
     7201 tttttgttgt cttggtattc ccactaccac aagctgccaa agagatgaca cttaaaacag
     7261 acacaacccc tataacaaga tttttcttta acataacatt aacttcctcc taatttcttt
     7321 atggccattt ttctaatcaa gaaatattat gaactaccaa tgtaaatttc tagttaattt
     7381 tctgtaaata attcgaaatt tgtgcatttc ggggtacagg gtcactggac atatccatca
     7441 agctaagaat aaagccccca tatggtcatg tatcccatct ccaaatctct taactcctct
     7501 gttttgatta taaaatagtt tattctgtgc tcctttcttc cataaatagg cccatgcaca
     7561 acgtgaggtt atattataaa caccacagcg cgcaaggtac tcaatcgttt ctagttcatt
     7621 gactgtccat acagaaaatg tcaatcctag ctgctgcccc acttcacggg ccaacttagt
     7681 taaccatcct tcatgaatat tgacgatttg aacgctttga taggctaatt tctgtaaggt
     7741 atcgatcaaa tctgcttcgg taatatcccc aagttttttc ccttcgaact gattttcaat
     7801 attataaaat atatgctttc cccaaatcgc agcccaagat aattgtacat agccactaag
     7861 aacaatttgt ggccagattt gataggtttg tgccagcgaa aaaacggcca tttccaatcc
     7921 ctcttctttt aaatcacagt taatggtcat ggttgccttc tcattggcca ctgcttgaaa
     7981 cacctgctca agggttaccg aatctgggac aacttcttac ctgtcatggg ctagtattag
     8041 cgtaccatct gtttgctgac gcacatctac ttcaaaacaa agctggcgat ccaaacaaaa
     8101 gcgaagaaat gcccagctat tctcctccgt attgtcacta ccagagtgtg cagtgatcat
     8161 ggtcattttg acccctcctt taattccgtg attttctccc acacgtccac tataccgatg
     8221 aagcgttaat ttcttgttaa tttcttgtaa attccctgcg ttttttcagt aaactagttg
     8281 taaatcttgc atgctccttc ctacgaactc atgaatcgtc gattttgccc ttctagctag
     8341 tttcaccaat taagtagttc tacattcccc gcttttaacc tactacacct ttttttgttt
     8401 tcagctattc cttcttcttg ctgaaagtcc ttgaggttct ttcttacagg ataccatcaa
     8461 ggtgttcaat ggcttattcc cattgaatga ccgagactta ctatcagatt tctgctcgtt
     8521 gaaccaagtc tttgatacag gatttcttcc gtcatcttct cacaaatacc accttgcgtt
     8581 tctctagcga ttggtcgatg tgcaccctcc cagtggactg tcactacccc tttttgctat
     8641 gcctagccca caaaaaaagc tgtcctcaca gacag
//
LOCUS       DARBDV010000110         8494 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000110 DARBDV010000000
VERSION     DARBDV010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.112"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..173)
                     /locus_tag="SCM56_002539"
     CDS             complement(<1..173)
                     /locus_tag="SCM56_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1111276.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="SCM56_002540"
     CDS             complement(593..1714)
                     /locus_tag="SCM56_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEG1111277.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="SCM56_002541"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="SCM56_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEG1111278.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="SCM56_002542"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="SCM56_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEG1111279.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="SCM56_002543"
     CDS             complement(4304..5215)
                     /locus_tag="SCM56_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1111280.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="SCM56_002544"
     CDS             complement(5232..6239)
                     /locus_tag="SCM56_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEG1111281.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="SCM56_002545"
     CDS             complement(6236..8365)
                     /locus_tag="SCM56_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEG1111282.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..>8494)
                     /locus_tag="SCM56_002546"
     CDS             complement(8370..>8494)
                     /locus_tag="SCM56_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG1111283.1"
                     /translation="NGQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2681 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DARBDV010000111         8252 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000111 DARBDV010000000
VERSION     DARBDV010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.114"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            235..1560
                     /locus_tag="SCM56_002547"
     CDS             235..1560
                     /locus_tag="SCM56_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEG1111284.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            1553..1903
                     /locus_tag="SCM56_002548"
     CDS             1553..1903
                     /locus_tag="SCM56_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111285.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            1900..2070
                     /locus_tag="SCM56_002549"
     CDS             1900..2070
                     /locus_tag="SCM56_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111286.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHKYEYYIGY"
     gene            complement(2974..3249)
                     /locus_tag="SCM56_002550"
     CDS             complement(2974..3249)
                     /locus_tag="SCM56_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HEG1111287.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(3221..4174)
                     /locus_tag="SCM56_002551"
     CDS             complement(3221..4174)
                     /locus_tag="SCM56_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEG1111288.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            4786..6279
                     /locus_tag="SCM56_002552"
     CDS             4786..6279
                     /locus_tag="SCM56_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HEG1111289.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            6414..6710
                     /locus_tag="SCM56_002553"
     CDS             6414..6710
                     /locus_tag="SCM56_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HEG1111290.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            6811..7662
                     /locus_tag="SCM56_002554"
     CDS             6811..7662
                     /locus_tag="SCM56_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111291.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            7683..7966
                     /locus_tag="SCM56_002555"
                     /pseudo
     CDS             7683..7966
                     /locus_tag="SCM56_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2866 a         1283 c         1553 g         2550 t
ORIGIN      
        1 atttttttct aatataacta agcaaaactg gataatacga cacttatttt caaattttca
       61 caagactaat agtaaatatc catttaataa agtgatacaa tgtgttgatt ttacaaatca
      121 acagaagaag tagggaaata acttacctat gcttcttttt ttgtacattt aatcgattct
      181 tctttacaaa aacgaacgta tgtttgtata atgtggaaga atggagtgtt atttatgatg
      241 gattattcaa aagagccagt aaatgattac tttttgatcg atatgaaaag cttttacgca
      301 agtgtcgaat gtatagaaag gaatttagat ccattaacga ctgaattagt cgtcatgagt
      361 cgagcggata atacaggatc agggttgatt ttagcttctt caccagctgc aaagaagaag
      421 tacggtattt ctaatgtcag tcgaccacga gatctaccgc aaccttttcc taaaacgtta
      481 catgtcgttc caccacgcat gaatctctat attaagcgaa atatgcaggt aaacaatatt
      541 ttcagaaggt atgtagcaga cgaagattta ttgatttact cgatcgatga atcgatctta
      601 aaagtgacca gatcaatgaa tctttttacg actgaaggaa caagaagtca acggagaaag
      661 aagctggctc aaatgatcca ggagaatatt aaagaagagc taggattgct tgctacagta
      721 ggtgtcggag ataatccctt attagctaaa cttgctttag acaatgaagc aaaacataat
      781 gatggattcc ttgctgaatg gacgtatcaa aatgttcctg ataaagtatg gaatattcct
      841 gaaatgacag acctttgggg tattggatct cgaatgaaaa aaagactcaa ccaaattggt
      901 atcatgagta ttaaagactt agctaattgg aatccataca ctattaagag ccatttaggt
      961 gtgattggct tacagctgta ttttcatgcg aacgggatcg atcgaacaga tatagcgatc
     1021 ccaccagaac cgacaaagga aaaatcatat ggtaattcac aagtgttacc acgagattat
     1081 accaggagaa atgaaatcga actggtggta aaagaaatgg cggaacaagt agctattcgt
     1141 atcaggcaac ataactgtaa aacaggttgt gtccatttat acattggcac gtcgatctta
     1201 gaaacgagac caggtttttc acaccagatg aagatcccta taacagataa tacaaaggaa
     1261 ttgcagaatt actgtctttt cttgtttgat aagtattatg aaggacaaga agtaaggcac
     1321 gttggaataa cctatagcaa gcttgtgtac actgattcgc ttcagctgga tttatttagt
     1381 gatccacaaa agcaaattga cgaagaaaat ttagataaaa ttattgacaa gattcggcaa
     1441 aaatatggtt ttacgtcgat tgtacatgct tcttctatgt tagaaggcgc tcgtagtatt
     1501 actagaagta cattggttgg tggccatgca ggcggcgcag gagggcttaa aaatgattga
     1561 ttataatgat cgtggacgtt taaagtgggc aggtttttat ttatctgatc atactgaaaa
     1621 aattgatgcc gacaatgaac aacgagccaa tcaaaatttg gcaaaagaac aaatgacgac
     1681 tgaagaggtt gcggaagtat tgaacgctgc agtcataaaa aatagatcag tatgtattca
     1741 aaaagaagaa cgaaacgctg aaggatacta tccagaagat attgttagca agatcttagg
     1801 tagtgatgag ctggggatct acgttggaaa aaacaaagtt cactacgacg aaatacgtca
     1861 tatcgaattt taccaggata ataaatgggg tcgattagaa tgaacattat ccagcaatat
     1921 gagctaaagt atataacctt tgatcagcta tcagaagaga tttggggtta tggccagcgt
     1981 ttgataaatg aggttggtgt tgaacgcttc tctttctatg tagaagctgc agcaggatac
     2041 cataaatacg aatactatat tggctattaa agtgcaataa aattatattt cttttatgtt
     2101 ttacattttg tatcaattct gataaaatta cctataggta attaaaaaat ataaaaaaac
     2161 tagttacaac cttaggacgt tgtaactagc ttgaagcata ctagagctaa ctaaaatagc
     2221 actagaaagc aatgttaaac ccgtccaaca aacttgattt aacattgaga ccagcttatt
     2281 cagctaaata tagttgaata agcttctttt gttttaaaaa aacaaaaggt gtactacgaa
     2341 taacgcaagt aaaagaaata ctattacaac cagtaggcta caagtaagta ttactaaaca
     2401 ttgcgtagtt agaacctttg ctctattgtc taaagccaca aaattagaga tcagctttat
     2461 gatgctaaga gcgagctgca tccatgcgga cataggttct tttgacacta ttaattctcc
     2521 tttttaggat aagggaaatt gctagtagtt ttttcattag caccatcccc cttcatttga
     2581 ttgagtctag tgctgctgtg atagtttgct gtttaatatg cttacattct tattataaca
     2641 taaatgaaac aaacacatgg ttagaggtcg tatcaatcaa aatagtgtct cacaaacgat
     2701 ttattactta ctcctgctga ctaacggact gttcttcagt aactgtaact atatgtttgt
     2761 taaaaccacc attacctgta gataaaaacc aatatattta ctatactcac tatattaaat
     2821 atatttagta tagtaaaaac tagaaacaat aaaatttgtg aatgttgtat acccccaaac
     2881 aatacacata ttttgaagga ttaggattaa taaagctaaa aatatagtaa gtatactaaa
     2941 tatatttact atatctagta tacttactat attttaatct tttgatcgtc tattatatat
     3001 aatccgttcc tcgtcgttta aagtatcaac ataggcgtcc attgcaagtt tgactatttc
     3061 atacatagga atatcttttg cataactaag atcccgaatg gtttttagta cgggaggatc
     3121 tacttgaact ggttttcttt ctttcgcagt gtactttcgt tcccctaact cgacattaat
     3181 atcatcttta attgaaatat cttcagcacc aattttcttc ttatctttgt tcgttggtaa
     3241 taatgccatt tagtttaatc tcctttccaa gaggtaggat atgattggcc attgagtcag
     3301 catatttagg tgtataatca aatccttcaa tatcaccatg ttgttcatag tattcaattc
     3361 tttcttctaa ttctgtaaag atgtctgcaa aaatagccca ccatcttcga tctacataat
     3421 catttaattg gataccattc tcaccataca tttctaaccg atccgagcct ttaacaatgc
     3481 ttttaaaaat gtttgattca ccatacttat caattgtttt ttgatagttt gcgtgttgag
     3541 aggaacgtcg cactgagaaa agaacaggca aaattccaat gatttgaaga tgagactctt
     3601 caaaattcgt gacaatcgga actaaaactt tattaataaa atcttctgtt ccttccattg
     3661 cgtaccgttt taattcttgc attgcaataa tataatctgc agcagttaag aatgcacgaa
     3721 ccacattatc tgttgatggt gggcagtcga taaagatata atcgaaattt tttctcaatt
     3781 cttctaaagg tttaatgaag gccaatgatc tcttacgacg atcaggatat ttttcggaca
     3841 agtattcatt aagttcttca gatccaggag agccagccat aatatataaa tgtggactta
     3901 cttgagtaat acctgaaacc cagtcccctt ttttgattgc tgcagacatg gatcgatcaa
     3961 agctagtaat ttgatacgtt gatccaatgt tggaagtagt atttctttgt tggtcataat
     4021 caattagcaa cacgtctttt tcaaaaagtt gactagaaac aatcgctaat aaatcattgt
     4081 ctgtagtctt accaatgccg ccttttctac ctgtaagcat tacaactttt cctagtttat
     4141 ctgatgtttt aaatgcagtc cgtaagtttt ccatagttcc ctccaatttc ctatactaaa
     4201 tatatttagt atacttcata tacttaatat attcgatata tctaatatac tccaaattga
     4261 taagaattca gactttataa ggaatatagt ggatatacta aatatatttg ctatattcag
     4321 tatatcagat atattcaaaa tgtcaaaata tatttgtgcg ttttgcgttg acaatttaga
     4381 gtgaaaagag gtaaacttaa ttctatagaa accacagata attcactatt tacaaagtag
     4441 caaaagcagt ggtactcttt aaacagaaaa attacataaa aaaaagagac ggaaataatt
     4501 gattttagca gaatcaatta tttctgaatc acatggttag gacatgtgtt cacgctcgtc
     4561 tcaagaatat gtaagtagct accaactact tatattaaaa gttgatcgga taccttttcc
     4621 gattgccttt attctaccaa aaaaaggcga gcaactcaac agaagaacgt ttatttagtg
     4681 tacttaaatt aaatattctt aaacactgaa gagagaacac atactatggc tgagattaaa
     4741 gctaaactgt gttctctttt tgtttgagaa aggacgttac aatcgatgtt gagcgaaatt
     4801 tattcagcta ttcttaaaaa cggcttacgt acatacaaat atcgaaacag caccatgaaa
     4861 cctgcaggtc atattgattc cagtaggagt ggtgctatct ttgcgtaccg tagcaagcaa
     4921 ttaatgaatg ttgggcgtgg tatggtcatt acttcggaag aagctatttt agagaacgaa
     4981 aagaaactaa cgcattggac acctaatacc tatcgctttg gtacttatgc ggatgattat
     5041 cgaacagtgg ttaaaggtca ttctgagaaa aacttaagtc aaatcaacac atttgtagtt
     5101 gatatcgatt caaaagagaa ccatcaaggc gaaattattt tagcctgttt agatcaagtg
     5161 ggctacatgc caacgctaat tttagaatca gatcatggtt atcaagttta ttttgtacta
     5221 aagacaccag gctatgtcac aaagaaaagc aatttcaagg tgatcgatgt agcaaaaaaa
     5281 atttcaaaaa caattcgtct acagttggct gaaaaattgt ccggagtgga tctaggatgt
     5341 aaccacttcg gaattgctcg attcccacaa gaaaataata ttgtttttaa agaaatgaac
     5401 tatcagtatt cttttgctga ctggattcaa tggtcgatga agcttgaatc agatcaaaaa
     5461 acaaaagaag atcgagaacg aaaactaatt gtctttcctg agaaaaaaga aattcgtcaa
     5521 gtagatgaac cgtggttcga tatgttactt cataaagcaa acatcgtcgg cggagaagga
     5581 cgtcttggtc gaaataatgt gattttcacg ctttctttag cttattactc ttcaggttat
     5641 gcattggata cttgcgaata caatatgttc acattcaacg aacggttaca ggaaccgcta
     5701 tctgaagcag aattagtaaa gattgtacgt agtgcttatt ctggtaatta tcaagcagca
     5761 agcagagaat tcattattga attgtgccag caatgggttt caccaacctt aaaaaataat
     5821 gatctgttca ttcaacgacg tggctggtgg aaatttaaaa agccacgaga tcaaagaaaa
     5881 tatagtcata aaaatgaatg gaaagaagat ctattggctt atctaagcga aaaaagtctt
     5941 gctagtgagc cttatttgaa cctgtcaaaa aaagaattgg cttcacaact aacgatgccc
     6001 aagcgaacac ttgataagat cttaaatgag ttacgagagg agaataaagt cttctacaag
     6061 gtaacacgag gaagaaatgg cggaatgatc attgcttcag tcaaaacgtt gttcgcgagg
     6121 gttattcaac taagtaaaga aaagagacta gattattcaa caaggatcac agaggtattt
     6181 ggactcaacg agagcttagt agaagagaca tttagacaat tatcaacccc cttgaaccct
     6241 cttaaagaag tgaatctttt tgaagtagat acaggttgaa aaataaaagg tgcaaattgc
     6301 cctatacaat atatctatga tacttctgta ggtgttgctc cctttgccta gctacgtgta
     6361 ggtggtatac ttataactgc gtatgcagtc ttatgtatca tggaggaagt tgtatgtcgt
     6421 taaaaaatta tgtctattca catcctgtta atgtgtttat cattggtcgt ttgggattat
     6481 ctgtggaagt gttctgtgag ctttatggtt ttaagcaggg gactttgtct tcttgggtta
     6541 ccagggaaaa aacagttgct tctttgccga ttgagtttct tcatgggttg tccttagctt
     6601 ctggggagac tatggatcag gtatatgatt gtttgtgtgt gttagagcag gagtatattg
     6661 agtataaaat agctaacgag ttaagaaaaa ggaagaagta tatccagtag tagtttttat
     6721 ttacttaaaa aattggttca ttgagcaacc tatcgctgct aaagtagcaa aggaaaacgt
     6781 taaaggaaag aatttaggga agaaatcgaa atgaaatcgg acgactggat tgaacacacc
     6841 tggcttcctc tgaaaagaaa tagcaaacga aagaaatttt gggtattgca attatggcca
     6901 ttaggaaaac tttgtagtta cggggcacga cttattcttg atttaaccgt ttactcgatt
     6961 ggagtatttg gaacggctta ttacttgata aatgatccca ataggattgc agcatcagac
     7021 atttttacag tgctaatgac gttgattttt gtcatgctat tgtctatttt tactcaatta
     7081 gtcatgcaat ttaccaggca aaaattaaca gcaggaaaac ttttcaaaag cctattcgat
     7141 ttaattgttg taaatgcaat tctagcgact ttaataaaac aaattgaaca atctggaaga
     7201 acaacaaaag gagtgaagga ttttttattg acgtttattc actttgtaaa tggacacatg
     7261 tatttgtttg aagtgggtat tattctcttt tttgtcgggt atgtatccac gaaacttatt
     7321 aaacgttcaa ttgaaaaaga aattgaaatt gacctgattc cgacacttct tggttatgag
     7381 tacaatgttg attttggaag atatgacgaa ttgatcaata cggatatcgt tcacacgagt
     7441 atccatgaat ctactgttaa gggcatcaga tctattttaa aaaatggcca ggaaatgaaa
     7501 gcaatagatc gaagaaaaga gatctacaat ttttatgaag tcgaagcaag cctacgatat
     7561 ggcatcggaa aattgtggtt agatggaagt aatattcgtc aaaacaaacc atacattttt
     7621 tggaacagta gtgatttaga acttccaatg aaagataaat aattatggag aatcgtttga
     7681 aaatgattcc tctatacagg attgtgctta tagttggcat ggagttaaaa accttaaaga
     7741 aggagcagtt attcaaagtg aaagaacgtc ataatcagtt actatgtagt aaaacgggta
     7801 cgatgaacat tgaataccga acagaatatt tagaattaac gaatcaaact gattttttgt
     7861 tgattcgaaa gcaacaagag caagaaaaaa attaatggct acttatagcc aattgattga
     7921 tgaatgggaa caattcaagg aatctaaaat tccagaacgt ctctaaaagc ctttatttgt
     7981 aaagagcata aggaaacgtg ggaacacttt ttaaaaaaag tgttccaata ggctgtgcct
     8041 attacgtttc tcaaaaataa atgtgggaac aaaatattta aaaataggat agtgaaagga
     8101 tagtgaagga tagtaaaagg agggtagtag gatagcaaaa aggataggct ccttttcgct
     8161 atccttttta aattggagga attaatatgg aagtaagaat aagaaatgtg gatgagaaaa
     8221 tcgtaagaga aattgatcga agatgtgacg aa
//
LOCUS       DARBDV010000112         8183 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000112 DARBDV010000000
VERSION     DARBDV010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.115"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            94..1248
                     /locus_tag="SCM56_002556"
     CDS             94..1248
                     /locus_tag="SCM56_002556"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEG1111292.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1270..2022)
                     /locus_tag="SCM56_002557"
     CDS             complement(1270..2022)
                     /locus_tag="SCM56_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG1111293.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2386..5052)
                     /locus_tag="SCM56_002558"
     CDS             complement(2386..5052)
                     /locus_tag="SCM56_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEG1111294.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5393..6844
                     /gene="cls"
                     /locus_tag="SCM56_002559"
     CDS             5393..6844
                     /gene="cls"
                     /locus_tag="SCM56_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEG1111295.1"
                     /translation="MVSSIITALYLLNALIALVAILINPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            complement(7173..7988)
                     /gene="thiD"
                     /locus_tag="SCM56_002560"
     CDS             complement(7173..7988)
                     /gene="thiD"
                     /locus_tag="SCM56_002560"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEG1111296.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         2379 a         1699 c         1457 g         2648 t
ORIGIN      
        1 aatccttaaa tattctcttt tttatatttc ttttaatctt tttatgatac cttagtaaag
       61 taagtttttt atcataagta aagaggtgtt gaaatgaaaa tagggattga tcgtctttcc
      121 ttttttattc ctaatttata tttagatatg acagagttgg cagaaagccg tggggatgat
      181 cctgcaaaat accatatagg gattggtcaa gaccaaatgg cagtcaatcg tgcaaatgaa
      241 gacatcatta cactaggagc aaatgctgcc agcaaaatcg taacagaaaa agaccgtgag
      301 ctaatcgaca tggtcatcgt cggaacagaa tccgggattg atcattcaaa agcgagtgcg
      361 gtaattatcc accatttact gaaaatccaa tcttttgctc gttcttttga agtaaaagag
      421 gcttgctacg gtggcaccgc agctttgcac atggcgaaag aatatgtcaa aaatcatcca
      481 gaacgaaaag tactagtcat agcaagtgat attgctcgtt acggcttggc aagcggtggt
      541 gaagtgacgc aaggtgtcgg tgctgttgcg atgatgatca ctcaaaaccc gcgtatttta
      601 tcgattgaag acgacagcgt atttctgaca gaagatatct atgatttctg gcgtccagat
      661 tatagcgaat ttcctgttgt tgatggtcct ttatctaatt ctacgtatat cgaatcattc
      721 caaaaagttt ggaatcgaca taaagaattg tcgggtcgag gactcgaaga ttatcaggcg
      781 attgctttcc atattccgta tactaagatg ggaaaaaagg cattgcaaag cgtattagac
      841 caaacagacg aagaaaatca ggaacgtctt atggctcgct atgaagaaag catccgttac
      901 agccgacgaa tcggtaatct ttacactggt tcattatacc tggggctaac ttctctactg
      961 gaaaattcga aatcactaca gccaggagat cgcatcggtc tattcagcta cggttctggt
     1021 gcagtaagtg aattttttac aggttatctg gaagaaaact atcaagaata tctcttcgca
     1081 caatctcatc aagagatgct agattctcga acacggatca ctgttgacga atatgaaacg
     1141 attttcagtg agacgcttcc tgaacacgga gaatgtgcag aatatacttc agatgtacca
     1201 ttttcgatta caaagatcga aaatgatatt cgttactaca aaatataata aaatcataaa
     1261 aagaaccctt cacgagtcgt gaaaggttct ttttttatct gcggaccaaa ggagcagtct
     1321 ataggtcagc ttagtcgtac taaggatttc tcgtttttca ctgaatagtt tgcgaatgta
     1381 cctggtcaca acaggatctt ttgcgtacgc gtcatagtat agcttcatca attcttcagt
     1441 aattgattcc tgtgtaaaat aagtaaaatc ttttttcttc actttgaatc cttcatcaat
     1501 taagtatgtt tcaaattgat ccattacaac tggcgatgta ttaggatctt cttctatcat
     1561 tcctagttgc ttttcgatag gataaaaaac atcatcgatt gattccacta ctctgattat
     1621 cacgcaccat ttgcttgact ttttcatcaa tttttctaat tgaataagat gtaccaatgc
     1681 tggatttgct gctgtaaaaa ccatttcata tgtctcattt gattggataa attcttctaa
     1741 cgactgtttg ataaaggaaa catttggcaa ctcatttttt tctgcttctt tttttgcatt
     1801 ttctagcatt cctgaagaaa aatcgatcaa atcatagcga ttaatttctt ttgctaagga
     1861 aagaaatctg ccagatcctc cagcaacgtc tgctgctgtt tcagcaggca ggactttctc
     1921 cgttttcaga taatttttta catcttcagc aatagttact tgcgattctt gctggatttc
     1981 ataatattct tcagcaaatt catcccattc tttttcattc atttgatttc ctctaatttt
     2041 ttgttatatg ttttcacttt tcggaataaa tcattctcct cttttatcta ttctatctct
     2101 tttgatgaat aataaaatga ttggctttcc gtctatcata aataaaaaaa gggactgtga
     2161 ccaaagtcgt gtcaaagtcc ctctttccga ataaacagtg tccaatcaga tgaccctcag
     2221 tattagctca aaatgctgat tttacaactt cttccttaaa cggtgttctc ctgccaactc
     2281 ttcggtcaca agtcacaata ttcaacaaac atgagaagct gtttgttgaa attgcacctt
     2341 gtgactcaga attaaacggc aggctcacaa cctcgttgat tcgttttatt tatttccttg
     2401 tactcgtaca atgatcaatt tagtgatttc catcaaaaca attgtcacaa gtgcgattcc
     2461 catacatgta aagacttctg tcaatccaaa tgctgatggg atagagaaga cttctttcag
     2521 tcctgggatc attgttaagc agtaaagcgc gctacataca actacagcta gtaagacact
     2581 tctgttagag aagaaccctg cttggatagc tgtttgtgtg tttgaacgtg ctgggaatgt
     2641 ttgcaatgtc cgacaccaaa tcaatgttga aaatgacatg gctacaccga attcaggtga
     2701 atgctgcatg ccaagccatt gagcaaagat aacagcaatc ccaatcagca caccacgata
     2761 cgttacagaa atcaatgttt tgtccgtaaa gattcctgaa tttggatctc ttggcgggcg
     2821 gttcatcgta gcaggttcag ctttttctgt acctaatgca attgctggca gtgaatcatt
     2881 gactaaattg atgaacaaca gttgtaatgc tgtaaatgga ttggaccagt cagcaactaa
     2941 ggcaaagatg atcgcaatga tcgcacctaa atttcctgca aatagataag aaatagcttt
     3001 cttgatatta tcaaacacat tacgtcctac ttctactgcg ctgacaatag aagcaaagtt
     3061 atcatccgtc aataccatcg ctgctgcatc ttttgccaca tctgtacccg ttcccatagc
     3121 aattccgatg tctgcttgtt tcaatgctgg tgcgtcattt acaccatcac ctgtcatagc
     3181 tgagatatga cctttatttt gccaagcacg aacgatacga attttattct caggtgaaac
     3241 acgtgcataa acagtgactt tttctaaaac ttcgtttagt tgatcttctg tcaattcatc
     3301 tagttccgtc ccggtcaaag ccaggtttcc ttcttcaaaa atcccgattt gttttgcgat
     3361 agcaacagct gttgttttgt ggtcaccagt aatcatgatt gttttgattc ctgcacttct
     3421 tgcttctgtt accgcctgag tcacttcagc acgtggagga tcgatcatag caagcaaacc
     3481 aacaaggatc aaatcgtctt catcttcaaa tgataaatca aaattatcaa gtggacggta
     3541 agcaaaagct agtacacgta atgcgcgtgt tgaaaaagct tcattttgct cttggaattt
     3601 tttcatcaat tcatccgtca tcggaacgac ttcaccattt agcaatactt ttttactgcg
     3661 ttggaagacg atatctggtc cgcctttaac aaacaagtaa tttttgttat caattgtgtg
     3721 tccagtagac attaattttc gatctgagtc aaaaggaagt tctgcttgtc ttgggtagtt
     3781 gtttcggatt tcctgataag gctggtttaa tttttcacta aagtcgataa acgctacttc
     3841 cgtagggtcg cccaatttag taccgtcttc actgatcgat gcatcattcg ctaaaacact
     3901 gatttcgatc aagcgacgtt cgtcagctgt ccattttgaa gggtcatttg tgaaatcccc
     3961 agtatcgcca ttacctaaaa agtagtcaac aactgtcatt ttgttttgtg tcaatgtacc
     4021 agtcttatct gtacagatga tacttgtcga accaagtgtt tcaactgctg gaagcttccg
     4081 aataatggca tgacgttttg ccattttatt cgttcccaaa gataacacga ttgttacgat
     4141 agactgtaat gcttctggaa tagcagctac ggctacggct acggcaaaca tcactgcgct
     4201 gacgatatct gcagtcatat ctcctgtacc ttctccaaga taaatacgaa acagttggat
     4261 acctaaaatc aataaagaca atgccaaaat agccaaactt agttttttac tgaatttatc
     4321 cagacctcgt tgtaacggag tgacttgctc agttgttgat tccaaaaggc cagcaacttg
     4381 tccgatctcc gtattattcc cagttgaagt cactaagaat ttaccgcgtc cgtaagttac
     4441 gatcgttcca ctgaatacca tatttgtacg atctccgatt ggtaccgtac ttgtgatatc
     4501 cgttacttct ttttctgcag aagtcgactc tcctgtcagc ataccttcat ctacttttaa
     4561 tgatcccgca tctagtaaac gtccatcagc aggtacataa tcgcctgcgt ctaacaacac
     4621 gatatctcct accacaagtt cgtttgccgg aatcgttttt tccttaccat cacgaatcac
     4681 tcgagcatcg ggcgctgaaa ggtttttcaa cgcttctaaa gaaccttctg ctttttttgt
     4741 ttggacaaca ctgattatag aattcaacat caatactgca aaaataacta agaattcgat
     4801 gtagtcaccc atgaccatct gaatcagtgc gacaatcagc aatacgataa ccattgcatc
     4861 tttgaaagtt tctaagaaca atttccaagt tggcttcttc ggcttttctt ttagctggtt
     4921 caagccatct ctttctaatt tttccttcgc ttgcgtggtg ctcaatccat cggttgagct
     4981 ctgcgtttgc tgcatcaacg agtcgatcgt ttctttatag accgcttgtt gtgcttgttc
     5041 cttgttttcc aaatttcatc ccccattttt tgatcttggc cctagtcctg tctgaaaaaa
     5101 aagagaccta agacaaatac gcatttgtct taggtctcac catttcatac aataccagaa
     5161 aaccatttgg ttaacttact gttgatatta tcgcaacaaa cgttgccggt tactccccta
     5221 aggagatatt caactgtact aatatcctac cttattaacc tacaaaaagc aactacttta
     5281 gcccgattta cctacatttt catgaaagga gtgaaaagta tattcacctt ggtaggcttc
     5341 tttcttctat catgctatac taatgttatt gtatgtaagg aggaacattt ttgtggtatc
     5401 tagtattata accgcccttt atctattaaa tgcacttatt gctttggtgg ctattttgat
     5461 caacccccga gatgtagcag ccatttgggc atggctctta gtattgtttg cccttcctgg
     5521 ggtgggcttt gtattatatt tatttttcgg acgtggatta acggataaga aaaaattcta
     5581 tctccgacaa agtgacttga aagaattaga aaactttcag tcttttagag gagataccat
     5641 tgaacattac gatcctgaca tgggcgataa agacaaacag caatttgttg acttcttctc
     5701 ttcattaaat cgtatgccgc tgacaagaat gaattctgtc actcttctca cagacggaca
     5761 agagaaattg gattcactgc ttcaagatct aaaaaaagcc aaacattcga tccatatcga
     5821 atattacgca tttgtgacag ataatatcgg ccagcaagtc ttacatgttt tagaagaaaa
     5881 agccgcagaa ggcgtggaag ttcgaatatt atatgatgca tttggctctc atggcacaaa
     5941 agcaaaagat ttcaatcgtc taatcaaaaa tggtggacat gtccatacat ttgttacctc
     6001 acaaagggca ttacttcgtt tccgattgaa ttaccatgat caccgaaaaa tcgttgtgat
     6061 cgatggaaag attagttata ccggtggttt caatattgcc aatcaatatg taaatacaac
     6121 aaaaaaattc ggctattggc gcgatacgca tatacggatt ttcggtgccg cttcttcttt
     6181 gctccagctt cgcttcttaa cagactggaa cgtctcggta cctgaagaaa aaaaggtcgg
     6241 ctatcatttg aattatttct ttaaaaaagc agatcgagat gaatctaagc ttgctgatac
     6301 atccatccag cttgtttcaa gcggaccgaa taacgaaagg gaacaaatca agctttcatt
     6361 tatcaaattg attacttctg ctaaaaaacg tgtttggata cagacacctt accttgttcc
     6421 tgatgaaagt gtcattgctg ctttaaaaat cgcaactgcc tctggtgtag atgtgaaaat
     6481 catgattccc aacaaaccgg atcatccttt tatttatcga gcaacacaat attatgctcg
     6541 gcagctgatc aaggaaaatg tacaaatcct tgtctatgag aacggcttcc tccatgcaaa
     6601 aacattgata atggatgatg aaatctgcat ggtaggttca gcaaatcaag atattcgtag
     6661 ctaccgattg aattttgaaa caagtgctgt catttacgat cctgagtttt tagaagaact
     6721 tgctactcag ttcaaagaag atgagacaca ttgttcatcc atgacaactg aaacagtcaa
     6781 ggaaatgtct aactggctat tattcaagca acaaatttct cgattatttt ctccaatcct
     6841 ataatttttt tattcaagta gagaaaaata tactaagatt aaagggactg tgacaaaaat
     6901 cttgtcacag tcccttcttc cgaacaaacg gtgttctcct gccaactcct cggtcacaag
     6961 tcacaatatt caacaaacat gagaagctgt tttttgaact tgttccttgt gactcggagc
     7021 tggacggcag gctcacgacc tctttcttaa acggtgttca aaagcttgca tctgcggtaa
     7081 taagtccaac caagttaaaa acataagaag ctgtttttac ttggctgttc ttattacttg
     7141 atgcagggca actttttcac aacttctttc ttttactcta attcaccagc tgcccaatga
     7201 ttgatggggc catatttgtg ccctacattt atttcttcac gaattgcacg gtttacatag
     7261 tcttttgcca atcggatcgc ttgttcaact gtttttcctt tcgctaactc agctgcaata
     7321 catgcagata gcgaatcgcc agttccgttg atccggtcgg taggataaaa tggtgcattt
     7381 aaccaaaatg attttcctga ttcgaacaat acgtagtcat tgactgtttc ttgctgcgca
     7441 tttttagcat gatgtccttt gatcataacg ttttttgcac ccatatcttg cagttctttt
     7501 gctgcttgca caaaatcttc ttcagctgct aatgtccgtc cagtcagctt ttctgcttca
     7561 taaaaattag gtgtcagtac ttcggccaaa gggacaagct tttctttcaa gctttgcaaa
     7621 gcagattctt ctagtaaaag atttccatgt ttcgttacga ttacgggatc cacgactaat
     7681 ggtccaaaat catatttttg gtaatttttt acaactgtct caatcaactt tgaatctgct
     7741 agcatacctg ttttagctgc acgtatttga aagtccttcg caatcagttc aaattctttg
     7801 tcgataaaat ctgttggcaa tgtcacactt gctccgattc cgtaagaatt acctgcaaca
     7861 catgcggtca tgacagaaac accgtaaaca tttcttgtaa agaatgtatg catatctgct
     7921 tgcatccctg cgcttccatc gctatctgat cctgcgatcg tcattgcttg tacatactga
     7981 tcttccatca aatcgattcc tccattttta taaaaagatt tttcataata ctgttactgt
     8041 ttctatcata tgtttttcaa agtatccctc agtaaatata ccactacctt ttcttggtaa
     8101 tcaaatttat ttgaaaaaac atttgcaata aatcagaaaa tagtggtcgt ttatttgtat
     8161 tcgctattct aataataaat aac
//
LOCUS       DARBDV010000113         7601 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000113 DARBDV010000000
VERSION     DARBDV010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.116"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(943..1356)
                     /locus_tag="SCM56_002561"
     CDS             complement(943..1356)
                     /locus_tag="SCM56_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEG1111297.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="SCM56_002562"
     CDS             complement(1812..2297)
                     /locus_tag="SCM56_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111298.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="SCM56_002563"
     CDS             complement(2436..2651)
                     /locus_tag="SCM56_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HEG1111299.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="SCM56_002564"
     CDS             2875..3675
                     /locus_tag="SCM56_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG1111300.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="SCM56_002565"
     CDS             3694..5562
                     /locus_tag="SCM56_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEG1111301.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="SCM56_002566"
     CDS             5555..6046
                     /locus_tag="SCM56_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HEG1111302.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /gene="srlA"
                     /locus_tag="SCM56_002567"
     CDS             6034..6588
                     /gene="srlA"
                     /locus_tag="SCM56_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HEG1111303.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..7601
                     /locus_tag="SCM56_002568"
     CDS             6606..7601
                     /locus_tag="SCM56_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HEG1111304.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2709 a         1213 c         1442 g         2237 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacataaaag aagggatata taaagtaatg aaacatttta agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgttcccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg a
//
LOCUS       DARBDV010000114         7507 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000114 DARBDV010000000
VERSION     DARBDV010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.117"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(57..1586)
                     /gene="ahpF"
                     /locus_tag="SCM56_002569"
     CDS             complement(57..1586)
                     /gene="ahpF"
                     /locus_tag="SCM56_002569"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEG1111305.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(1596..2159)
                     /gene="ahpC"
                     /locus_tag="SCM56_002570"
     CDS             complement(1596..2159)
                     /gene="ahpC"
                     /locus_tag="SCM56_002570"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEG1111306.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(2303..2965)
                     /locus_tag="SCM56_002571"
     CDS             complement(2303..2965)
                     /locus_tag="SCM56_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1111307.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            3149..4795
                     /locus_tag="SCM56_002572"
     CDS             3149..4795
                     /locus_tag="SCM56_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEG1111308.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            4786..5868
                     /gene="uxuA"
                     /locus_tag="SCM56_002573"
     CDS             4786..5868
                     /gene="uxuA"
                     /locus_tag="SCM56_002573"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEG1111309.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(5976..7496)
                     /locus_tag="SCM56_002574"
                     /pseudo
     CDS             complement(5976..7496)
                     /locus_tag="SCM56_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331751.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         2358 a         1370 c         1385 g         2394 t
ORIGIN      
        1 aaaattcttg tcaccatcct tttttccgct ctctgacttt ttacaatctt catagctcaa
       61 gtgcgcatca gataatcgaa tgctcctaaa gctgcagtag ctcctgaacc cattgaaaca
      121 atgatttgtt tgtatggact atctgtacaa tcaccagctg cgaaaacgcc ggggatattc
      181 gttgcgccat ttttctcggt cactatttca tttcttggat tcatttcgat cgttccatct
      241 agccaatccg tattagggag taagccgact agaatgaata ctcctgaaac agctagtgta
      301 tgcatctcca tcgtacggcg atcgatatag gagagagatt ctacctgatt cgtaccgctg
      361 atttctttcg tcgctgcatg tttgataaca gtgacattcg caagagagac tagtttatcc
      421 tgtaagattt gatctgcttt taattctggt aagaattcta gaacatatac atgttttgct
      481 aacccagcca aatcgattgc tgcttcaaca ccagaattac caccaccgat aacagcaatc
      541 tcttttcctt taaataaagg gccgtcacag tgtgggcaat aagcaattcc tttattttta
      601 aactcttttt cgcctggtac attgatagaa cgccagtgcg cacctgtcgc gatgatggcg
      661 gtttttgctt taagtgatat accgttagct aagccaatat taataaaatg ttgctcttta
      721 tttaaggaaa cggcctgttg attcatcata atgtcgatag ggtaactacg cacatgactt
      781 tctacttgct gcatcaattt tggaccttct gtgtaagagg taccaatcat attttcaatc
      841 cctagtgttt caacgacttg tccgccgaaa gaatcagcga caagtcctgt acggattcct
      901 ttacgtgctg catagatagc tgcactacta gctgctggtc caccgccaat cactaataca
      961 tcataaggtt ctttttcaaa caactcttct tcaatcaaag ggccagtgat tttttcaaca
     1021 atttgttcga tcgtcatgcg tccgctacta aattcttctc catttaaaaa cacagttggc
     1081 actgccatga tttttttatc atcgatttct ttttggaaca tcccaccttc aatcatcgta
     1141 tgagtaatat ttggatttaa tacagctaat atattcaatg cctgtacaac gtcggggcaa
     1201 ttatgacaag tcaagctaac ataactgtca aaatgcaatg tttgtgttat ttttttgatt
     1261 ttaagactca aagattcact gattttcggt gcccgtccac taatttgtaa catagccagt
     1321 aaaaaagagg aaaattcatg accgaggggg ataccagcaa aaacgattcc actttctttg
     1381 ccaactggat tgatagaaaa actaggtgtg cgcatcgaat ctcttttttc ccaagagatt
     1441 ttaggagaca tttctgctat ttccttcgta aattcagcta gtttttgact attttcacta
     1501 ttatccaaac taacagaaaa aacaatagga gattcaatca gtgctaaata ttgttcgaga
     1561 ttttgtttag tttctttgtc taacatatcc accacctaaa tctttccaac aagatcaaaa
     1621 cttggtttca atgtttcgcc actttctttc cattttgccg gacaaacttc acctgggtgg
     1681 gtacgtacat attgagcagc gcgaatctta tcaatcagtg cactagcgtc acgaccgatt
     1741 ccatctgcat tgatttccat agcttgaacg attccgtctg gatcaataat aaatgttcct
     1801 cgctgagcta atccttgctc ttcatccaaa acatcaaaaa tacgagaaat ctgatgagac
     1861 ggatcaccga tcatcacgta ttcgatttta ccgatagcat ctgatgtatc atgccatgct
     1921 ttgtgagtaa agtgggtatc ggtagagcaa gaaaagactt caacacctaa agatttcaat
     1981 gtactgtatt gttcttgtaa gtcttctaat tcagtaggac aaacaaaagt aaaatcagct
     2041 ggatagaagc aaagaatact ccagttcccc ataagatctt tttcactgac ttctaaaaat
     2101 tctccttgat gataagcatg tgcattaaat tcttcgattt ttttcccaat caatgacata
     2161 gtaaaacctc caataataat tatttattta taattattgt aatttagttg gagttgcaaa
     2221 gtcaatggat attttgttat ttaataaaga ttactcaaaa acgaaggtta attataaaaa
     2281 tagaagaggt cgcaagaaaa tgctactgct ggaaatactc gggataacgt tgagtaacag
     2341 tttcctgttc tttcagcatc atgtgtaagt gcaatcccgt ttgaaatcga gcttcttctg
     2401 gttttttatc aactaaagcc ttgagtaaat cataatgttg ttccatgatc gtttcccatg
     2461 gaagcccctg aatggtcact cgtaaccagc ggaaacgttc taaatgggta ttatggtcat
     2521 ctaaccagtt ccaaacttct tttcttcctg caatttcgaa acaagcttca tgaaataaat
     2581 tatctgcacg gaaaaaagcg cgttcgtcat ttattttgac tgctttttct tgttcttcta
     2641 aaatcgtttc tagcactatt cgtgtttgtg tgtccaattt ggaacaacat tcaatcatga
     2701 tttgctcttc caactgttct cggacaaagc gagcattttc tgcagaaaca agattaattt
     2761 tggaaacata cgtaccactt tgaggtatcg tatacacgag ttcttgctga cgtagttgaa
     2821 tcaatgcttc gcgaatggga gtccggccga tagcaagatg ttcctccagt gccttttcag
     2881 atattttcct tccaggttct aagtcagcat agataatttg tttacgaatc gcttgataag
     2941 cttgtgattg caagctgctt ttcatcttta ttcctccaaa atcatttcta tgaacgtagt
     3001 ataacagaag aggtctgaaa aacaatctgg tatgtcacat taactagtaa atttgtataa
     3061 atagtaaaat tttggttgac atagcgattt ttattgcata aaattcactt gaatacaaaa
     3121 actaatatgt tagtttagga ggggaaaaat gattgattta aacaactgga ggaactttat
     3181 ccctgaatta tctcaaaaag aagtagaaat acctcatttt gaccgtgcat cattaagaga
     3241 aaatggacaa aaaaatcctg tctggatcca tttcggtggc ggaaatcttt atcgaggatt
     3301 tcatgcagaa atcgctcaac gtttagcgga tctaggcgaa ttgtccactg gtgtcattgt
     3361 ttgtgaaaca ttcgatgaag aaataattga aagggcttac caagattttg atgatttgat
     3421 tcttgaagtg gtcatgccaa gcagtggaga gttcgtcaaa cgtatattag ctgcgacagc
     3481 tgacagtctg tattgtcatc cagattgttc tgaacacttt gataagatga ttcgacactt
     3541 tgaaaatcct agtttacaat tagttacttt tgctattact gaaaaaggtt atcgattgaa
     3601 agatacaaat aataaatata taggaccgat tgcagtcgat ataaaaaatg gtccaaagaa
     3661 gccatcacat acaatgagta ttcttgctgt gctactttat aaacgttttt tatctggagc
     3721 ttggccgatt gcacttgtct ctacagataa tttttcacaa aacggtagtc aattacgtac
     3781 ttctttgcta acgatcgcaa aagaatggca aaaggaaggt tatgtacaaa atacatttat
     3841 ccaatactta tcgaatgaag aaattgttgc gtttccatgg acgatgattg atcgtatcac
     3901 cccgaatcct tccttgtcag ttagcaaaat gttggcaaaa gagggctttt caaatatgtc
     3961 gattttccaa accgcaaaag gaaataatat tgctccattt gtcaatacag aggacatcca
     4021 ttatttaatc atggaagatc attttcccaa tggacgtccg aaattcgaaa aagctggggt
     4081 attcttgact gatcgtgcaa cggtagataa agcagatacg atgaaggtga cgacttgcct
     4141 taatcctttg catacgtctt tagctattat gggttgttta ctggggtata ggagcatagc
     4201 tgaggagatg aaagatccag atttatcgct gctagtttct gaaatcgggt acaatgaagg
     4261 cctcccagtt gtggagtctc caggtatcat tgatccaaag atctttctgg aagaagtaat
     4321 tactgtgcgc ttttctaatc caaatattcc taatatgcct cagcgaatcg ccacggatac
     4381 ctctcaaaag gtggcgattc gttatggtga gacgattaaa aaatatatgg aaatagaagt
     4441 taaagacccg cagacacttg tattcatacc attgactctc gctgcttggc ttcgatacct
     4501 tattggcatt gatgatcaag gaaaaccatt catcccgagt ccagacccac tattgacaga
     4561 ccttcagcat aagctttccg atattcaact gggtacgcaa gatacatcac atattcatga
     4621 gaaaatggct cctattttgt caaataaaga aatttttggt gtagatcttt accaagcaga
     4681 gcttggcaga aaaatagaag aaatgttcat caagatggta gcaggaccaa atgccgtacg
     4741 aaaaacatta caggattact tacaagaaca tggaggaaat ttatcatgga aatgactttt
     4801 agatggtacg gatcacagga agaaaagatt accttagatg aaatcagaca gattcctgcg
     4861 gtcaaaggga ttgtaggcac attgatggat ataccagtag gagaggtatg gcctagggag
     4921 aggatcaaag aactaaaaga agaaattgag tctcgtgggt taacactaaa agtaattgaa
     4981 agcgtcaatg tgcatgatgc cattaaaatt ggattacctt cccgtgagca gtatatcgaa
     5041 aactataagc agacaatccg aaacttggca gaatatggaa tagaagtcat ctgctataat
     5101 tttatgcctg tatttgactg ggtgaaatct gaccttgact accgcttaga agacggctca
     5161 tccacgttag cttttatatc tgatgatatt ccagatgatc caagagaaat cgtcgcgcgc
     5221 atcgaacatt ccagcaatga atttggattg ccgggatggg aacctgagcg cttagctcct
     5281 atcaaatcct tgcttgaagc ctatgcatca gtagacgaag aaaaacttcg agaaaatttt
     5341 gcctattttc tccaatcaat catcccaaca tgtgaagagg taggggtcaa aatggctgta
     5401 catccagatg atccgccata ctcgattttt ggattagctc gaatcgtaaa gaatcgagaa
     5461 gatttagatt ggttgtgtcg catagtagac agcccttata acggaatcac tctttgtaca
     5521 gggagtattg cagaagatcc agaaaatgat gtatatggga ttttagcaga attttgccaa
     5581 agagatcgaa tcccatttgc acatgttcga aatattaaat ttttagctaa tcgagatttc
     5641 tatgaggcac cacataaatc cgaatacggt tcattagaca tgtaccgcat actaaaaaca
     5701 atgtatgaca acggatttaa cggatacatc cgtcctgatc atggaagaat gatctggaat
     5761 gagacaggaa gaccaggata cggcttatat gatcgagcat taggtgctgt ttatctaaat
     5821 ggattatggg aagcaatcga aaaaggaaac aaaagtaaag ataattagtg aaaaaaactg
     5881 taattttatt atgtaaataa gaaggatgtt caaattcttg atacataaat ccggaggttg
     5941 ttaataagca ttcggattta tgtatcttat ttggtctagg aagtaacttg attttctttt
     6001 ttcgattgat ccatgagtgc ttcttctttt aattcaaaga tatattctga gatcctcata
     6061 atcacttttt tccagtcttc tggtgtgatg attcctttta aaatatagat ttttttgata
     6121 tgagaaggca attgtttcaa agggcagtcg ctgataacga tgttgcagtt ctccataaaa
     6181 tcattttgta ttttgatcga ttcatttgaa aaaatttgtt ctatcttaac gatcagatct
     6241 ttactgatat aaaaatcacg cgtatagttc acaccaatca caattcgcgg ctgttttttg
     6301 tagcgatcat acaaatgtac taggtcttca accatccatt gcacattttc cttttctaaa
     6361 ataactggta acaagtgttg attttgttct ctgaaatcca tataaaattg ttcaatggcg
     6421 gtttggactt ctttcaatac gctatgttct tgataaagca tgaaagtagg aaggggcgta
     6481 tttggttgat tgaagttaag aaatccatta taaagtttat ttcgtgcgaa tgccaatgag
     6541 taaagaatct tttctttttc aggtataaac aaatcaaata cagtacaaag ggactccacc
     6601 atttctcttg aataaagaac atgaggaacg tttgcttttt ttaaaagttt gtattgctcc
     6661 aagcttgttt cttccgattc gattttcgtg atagaaagtc gagctaaaat attcactaag
     6721 atgcgttgtt cgcgacaaat tggcagatta ggtcgcaaaa tatctacaga gagatcaata
     6781 aaaaagtcaa tatattcata ctctgtgaat tctttttgta cgtctttcac agaagttgca
     6841 ggaaaattct ctttacatat ttttaataga aaatataatt tatctatcat accgctattt
     6901 agtcggtcaa gtacttcttt tttaaaaaaa gtattcatca taaaagccac tacttgcgga
     6961 tcttcatcat aaaattctgt agcagtactt gaaaagatgt tccaataaat atctaatgct
     7021 gcatattgaa tttgtacctc ttttccagaa aaagactttc tgccattatt agaaaaggag
     7081 atagacaaac cgtactatgc taaaaatgca ttaatctttt tcattttcgt atacaaataa
     7141 gaggcactat agtttgtttg ctgactgaaa ctgataatcg aatttgtctg acagctgaac
     7201 aaagaagtca agatctgata actgactgat tcgttgcagt atcttccaaa tagacgataa
     7261 tagcagtaag ccggatcaat tccttcaaaa ttcaacttta aaaaaggttt ttgcttatca
     7321 atatgcagtt ctgttagatt gagacgctca aagtcttgtt ctaattcttt gatgtatagt
     7381 aaaaaatttg attcagacat atggaaatga tctgctaaat attgtgtagt aataaagaaa
     7441 cgatgttcag ataggaaaag aaaaatatct aattttgtat tgcttttttt cttcaagata
     7501 aacgttt
//
LOCUS       DARBDV010000115         7375 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000115 DARBDV010000000
VERSION     DARBDV010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.118"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..708
                     /locus_tag="SCM56_002575"
     CDS             <1..708
                     /locus_tag="SCM56_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111310.1"
                     /translation="KEAKNLCIFVLLLHGRVINLVNRKSTTKFANSFWFLKNINKTVE
                     KNGVYKGDFHFQNAETIEREHHYFDLLVNTVVELAKWAKSNETYSQVLKRIMSQETSF
                     IYELSKSNLKYTYNEDELEILDNPENSQWIKELKREDFIETLRILKSLFDLKLAKATI
                     MGIERDSYYWEEIGLYAPQIEGEDKEKRIDNIINYINSLSEPLKCRGLDFIGTLGWTE
                     TYYEKIYKEIIEQYFPK"
     gene            891..3566
                     /locus_tag="SCM56_002576"
     CDS             891..3566
                     /locus_tag="SCM56_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HEG1111311.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            3748..3987
                     /locus_tag="SCM56_002577"
     CDS             3748..3987
                     /locus_tag="SCM56_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111312.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            4003..5376
                     /locus_tag="SCM56_002578"
     CDS             4003..5376
                     /locus_tag="SCM56_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1111313.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            5380..7014
                     /locus_tag="SCM56_002579"
     CDS             5380..7014
                     /locus_tag="SCM56_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1111314.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTNLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            7177..>7375
                     /locus_tag="SCM56_002580"
     CDS             7177..>7375
                     /locus_tag="SCM56_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111315.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2634 a         1143 c         1461 g         2137 t
ORIGIN      
        1 aaggaagcta aaaatttatg tatttttgtc ttattattac atggaagagt tattaactta
       61 gttaatcgaa aaagtacgac taaatttgca aactcattct ggtttttaaa aaatattaat
      121 aaaacagtgg aaaagaatgg agtttataaa ggtgattttc attttcaaaa tgctgagact
      181 attgaacgag aacatcatta tttcgattta ttagtgaata cagtggtaga attagcgaaa
      241 tgggcaaagt caaacgaaac ttatagtcaa gtacttaaaa gaattatgag tcaagaaact
      301 tcatttatct atgagttaag caagtcaaat ttgaaatata cttataatga agatgaactt
      361 gaaattttag ataatccaga aaattcacaa tggattaaag agttgaagag agaagacttt
      421 attgagacac taagaattct aaaatcgtta tttgatttga aattagccaa agctaccatc
      481 atgggaattg aaagagattc atattattgg gaggaaattg gactatatgc tccccagatt
      541 gaaggtgaag ataaagagaa aaggatagac aacattatta actatattaa tagtttaagt
      601 gaacctttaa aatgtagagg acttgatttt ataggaacat taggatggac agaaacatac
      661 tatgagaaaa tatataaaga aataattgaa caatattttc ctaaataatt taataaggat
      721 tgatttatcg taaaagaaaa aagttgtgat ttccaaaatc acaacttttt tcttttgtca
      781 agattttgag tgggatatat ttttgtagaa ctgaagatca tgaaattatg agaggttgga
      841 ggtaatgtag tcatacaaat aaaaattttt tagaaagtga gtggtaatta atgaaaatga
      901 atcacacttc aattcagaag catttacaac tgttaaatac actggggatt tcttatgtta
      961 atttgagaac tttttctaca ttaaacaaga aaaccgtttc tcatattttt tctactgcag
     1021 attctcaagc aattattaac tatttagaaa aacagaaatt gtctgaaggt acagtaaaca
     1081 taacttataa tcctattaaa caagaggtga ttacacaaaa aaagcattct gtgagagaca
     1141 atgatattga aaaaattcgg tttgtattta ttgatattga ccctaaaagg aaagagggaa
     1201 gtgcaacaga tagcgaaaag aaaaaggcag aaaatgttat ggaacaagta gaacgttatt
     1261 tgaaagaaaa gagaatagaa tcatttgtaa aagttgatag tgggaatggc taccatattt
     1321 ttcttcccat aaatgaacaa ccaaataatc acgagacgat tcttactatt cagaattttc
     1381 ttcaattact tcaccgaaga tttggagtag aagatgaagt tgatattgat acttctgttt
     1441 ataatccttc aagattatgc aaattagttg gtacaccagc ggtcaaagga aagtccacaa
     1501 aagagcgtcc gcatcgttta tctagtttcc tttctattcc cgataacata gatagaagta
     1561 attgttttaa aattcttgaa cagatagttg aagaaaattc catgatgtca acctctagag
     1621 taaaaagcca tccatctttt agagaagtgt taccttttat tcaagcagac tataaacaat
     1681 ggttagctca ctatcctgaa ttaacttata ccataaaaga gggcgatcat aaaggagtta
     1741 ccttacttat atttgacgaa tgtccattaa gaaaacacac gaataattct aatggttctt
     1801 gtttgtctgt tacggctgat aataagattc gatttcattg tctgcatgct tcggataaaa
     1861 ataaagatat tcaagacttt gtaaaaaaat atccgttgcc aaaacctgta aatccttctg
     1921 tagaatcatt agaatccaat caagaatatt attttgacca gtttaaactg aatcaagaag
     1981 gagtattttt ggtgaaagaa ggtcaagaag aaaaagttgc ttgtccgatg tttattaagc
     2041 aaatacgcaa aaatatcgaa caaaaagtca ttacacttgt tcttgcctat tatgtcgatc
     2101 attgttggga tgaaatagaa atagaaggtg gatgtttaca aactaataat tttaaacaat
     2161 tagcaaaaca tggcttaatc attaaagcta gaagagaagc tgaagtcgtt gactttttac
     2221 agattcaacg ggagaatgct ccaatctttt ttgaacatga atctcttggc tggaaaaaag
     2281 aaaatgaagc aatgattttt cagttacaag attcttttgg acgtgaaatt ccttctgtat
     2341 taactaaaaa ctcatgctac caattaacaa ctaatggtac agaagcggaa tttgacgcat
     2401 tggttcataa gtatgtttta ggaacgaact tagaacttgc ttggtcgatt ggtattgctt
     2461 ctattgtgtt aggctattta aacgttacga aggctaatac ttttttgagt ttactaatta
     2521 acctacaggg caagagcaca acgggtaaaa ccactacgtt acactttatt gcctctttat
     2581 ttggaaatcc aactacggtt cttagaaata tgaatgctac caataaagcc attataaagc
     2641 tagcatcaaa caatcaggga ggtgttccac taatactaga tgagttagga gcagcaactc
     2701 aaaataactt tagctcgttg atctatcaat tagcttcagg agaagaaagg ctaagattag
     2761 attctaattg tcagcttatg ccacaagaga ctttttctgt tttgaccctt acctcttctg
     2821 aagagcgttt ggaaaattat ttcaatgaga aattaactgg tttgaaagtt cgatatatcg
     2881 aattttctga tatcaagttt acaaaggatg cgaatcacgc agagaaagtt aaaagtcttt
     2941 cttctaatac ttttggttgg ggtataaagt tatttctttt gaaactcttt gaacaaggga
     3001 ttacaaaaat tgaagcaact tttgtcgaag ctcaaaaaaa actatgggct gttttacctg
     3061 aacattcttt gaagcaacgc attggtaata atctagcttt gatttatagt ggagccctcc
     3121 taattcaaga tatactgggc tatcctttac gattagagca tatacagacg gtgcttatta
     3181 attcgtacga acagttaatt gtagatacta gtttaggaag tgagcaaacg gtatttgacc
     3241 gagtgaaaac attgttggtg atgaatagcc ataaattttt aacaaacagt gacaacaaac
     3301 atacgcccag tacgatttgg ggaaaagtat caattcaaaa aaatggggat attaaggtaa
     3361 atatttttcc tatcccgttt gaggacttgt taaggcgaga atttcaagta aaggatctaa
     3421 cctatctttt taggcaattg cttgattcgg gtaatatgtg tgcagaaaaa gggagaagaa
     3481 cgaaacgtat tcacatgaat cgtcaagttc ttccaacata cgagattcta ttaccttctt
     3541 cactcaaatc gtattttcga ttgtaattta gaataaacca aagtatgaaa gtgaggcacc
     3601 atgttatgga aatatttctt attgatttag caaaacaact gattacttat ctgtatgata
     3661 gcgagcaaac ccaaacggaa gtaaatgaag cttgatctat gcttcattta cttcattcaa
     3721 tcaaacatga aagaaaggaa aattctaatg actaaaaata aaatgacact aaaagcagag
     3781 gttcttctgt atattcaaga acatttttca aatcaagcct tttttacaaa accaatttac
     3841 ttggatttcg aaataagagg ggtatctgct ggatctatcg ggggtactct tcaagcgttg
     3901 aagaacgaag gatatttaga aaatcacttt gtgcaacgtt cgttcaatag aagagtcgtg
     3961 aaaaaatggt atttagttca ttcatagaga ggggaatcaa gcatgcgtgt ggcttgttat
     4021 gatcgaacgt ctgtttatga agaagcgaaa attagtaatg acgctcaata tgatgcagtg
     4081 aaagagtatg ttttagagaa atatgaacaa attccccaac gattttcaga caaaggagaa
     4141 tcaggggaaa aagcggagcg tccagctttt cgacagctta gagcttgggt ttgtgcgtca
     4201 gaagaagagg gggtgatcta tgtttggcgt tatgatcgat tggcacgtca agccaaaata
     4261 gccttagaat ttattgagtt gtgccaacaa catcaggttc aagtgtattc gatttctgaa
     4321 cctcttcctg cttctttctc acacgcgtca acgaataaac tatttgtgca attattgttc
     4381 acttttgcag aacaacaacg aaacgtcatt attgagaatg ttcgtgccgg actttcctat
     4441 aaaaagcaac aacggcaata tttgtcttct gcggtaccat atgggtatcg tttagtgaat
     4501 gggaaaatcc aacaagaagt acacgaagca aggattgttc gctatatttt tcaactttat
     4561 ttgaccaaaa aatatggcta caaaaagctt tgtcaacgat tgacgcaaca aaagcttttc
     4621 ttccgcgaac gtccttttca gccctatcat atttatagta tcctaaaaaa tccattgtac
     4681 tatggcgaaa tcaaaggagg atctttgggg aagtatctag gaacctttga accgattctc
     4741 tcgaaagcaa cctttttaca agcccaagag attcgccaat caaggtgtac agctaaaaaa
     4801 gatacgtatc tgtacttatt acgtcaaaaa attcggtgtc ccttctgtgg tcgtcatctg
     4861 agttcgaagt atcaatggaa tacaaagaag acgaagacgc ttcattatta tcattgtacc
     4921 gaccgaagct gccaaggaat ttttcttcgg gcagaaaggg ttgagtcaga agtattggaa
     4981 aagatacagg ctttttttca atacgaaggc gtctatcaag ccttgattgc ccaattaacg
     5041 caacaattag ccgaaaagcg aaaagatgaa aaacgcaaag taatccaaca aaagaaacaa
     5101 gaagcacaat ggattaaggc atttgaagag ggcaattgtt cactggaaga actcaagcaa
     5161 caactgcaag ctcttcaaaa gccgatgtct acttcttcgt ttctttcact ccaacagtgt
     5221 gaagcaaaag tgaaagaatg gcttcaacta aaagacctct ctagtcgaca attattatgt
     5281 gactatgttt gtcggattga ggtaacaaag gataaaacag tttgtgggat ttacttccga
     5341 caactgacac aaaatatttt agaaaggaac gaatgatcta tggaaatgat acaaaccaaa
     5401 cgagtcgcat tgtatattcg cgtgagtaca caagaacaag ctttagaagg acatagtatt
     5461 caagcacaag aacaaaatgg acgacagttt gccgaacgga tgggctatga agtggtagaa
     5521 gtttatgccg atgaaggaat cagtgggaaa tccacaaaaa atcggttcgc ctatcaacga
     5581 atgatgaacg atgctcggaa ccatcgtttt gatctactag tgatttggaa attgacccgt
     5641 ctcggacgca atatgttaga tgtcttacaa acagtcgagg aattattgcg atatgacgta
     5701 ggattacatt cgatctcgga acaatttgac gtgaccactt cgacaggtaa attgatgtta
     5761 caactccttg ggagtttcgg tgaatttgaa cgcaaccaaa ttgcggagaa tgttcaaatg
     5821 tcaatgcaaa gcttagtgaa agataaaaag cgttatgcag gaggccgacg tttaggctat
     5881 gtcagtggta ttgacacgaa tggtcaaaaa caattggtca ttgaaccaga agaggcgaaa
     5941 attgttcgtc tgatctatgc aaaatattta ggcggagcag ggtatcgagc gattgccaat
     6001 tttttgaatc gtcaaggata tcaaacagtc aaaaagaatg ctttttctac cacggcagtc
     6061 aaagatatct tacgcaataa aatttatgca gggtatttag aatatgcgcg ctacatcaat
     6121 tgggaaacca aacgacggaa ggggaagaat ccgagaccga ttttagtgaa aggaacccat
     6181 gaaccgatta tttcggaaga agattatcaa aaagtacaag aacgccttgc tttagaagga
     6241 aaacaaccca aatggaacca tacaggtgaa aatgtattga caggcttgtt acgttgtcca
     6301 gaatgtggcg caccgatggc agcgagtaat gtcacaaata ccctaaagga tggcacaaag
     6361 aaacggattc gatattattc ttgttcagtc tttcggaata aaggagcaag tgtgtgtcat
     6421 gccaattcga ttcgtgccga acaagcggaa acatttgtcg ccgaccgatt aaaggagatc
     6481 gttcagctac cagaagttct tccccgattg gtggcagcgt taaatgaaga aatagtgcga
     6541 caaagccaac cattggaaca agaactcgtg gtgttactgg aacgaaagga agaattgaag
     6601 actaaaattg aaaaatggga agctgctttg gaagatagtc ctgaattgtt tccgatgtta
     6661 aaagaccgct tagacgaatt gactgaaaaa cgacgacagt tgcacattcg agaaaatgaa
     6721 atcttaggga tctttcaaca aggagagccg attcaagtca aagatgtcca acggatattg
     6781 actaatttgg atcgttttct tgcccagagt gagaagaagc aaatcaaagc cttgtatcgt
     6841 acctttatag agaaaataac ctttgatcct aaccataagg agatgttgga aattacgatg
     6901 aaattcacgc atgccgtggt acaacaattg aatgagcaat atcaaatagc cgtatccaaa
     6961 acaaaggata cggctatttt tgtgttaaaa acacccttta cgttaacgat ctagtctagg
     7021 ttatatcaaa caacgttaga ggggcgctaa cgcttctcta gcgatttgaa atcgttagag
     7081 aagtagttag ggaggtcctt atcataaata gggatattta ttaatgaata tttgaaatat
     7141 aattgtatat aagaattaaa aagagtgggg atagatatga aaaagatggg taagtgtatt
     7201 ttatgtgaaa aatacactga acttactaaa gaacatgtac cacctaaaaa atcagggaat
     7261 actggaggta aaaagaccag aactggtaat ttagatgatt tccttaagag cgattttaca
     7321 aaaggtgatt ttccgaaggg gattaaaagg aaacctcaag gaaatgttta ttata
//
LOCUS       DARBDV010000116         7351 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000116 DARBDV010000000
VERSION     DARBDV010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.119"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            138..2747
                     /gene="clpB"
                     /locus_tag="SCM56_002581"
     CDS             138..2747
                     /gene="clpB"
                     /locus_tag="SCM56_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEG1111316.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2898..3584)
                     /locus_tag="SCM56_002582"
     CDS             complement(2898..3584)
                     /locus_tag="SCM56_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111317.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3562..4056)
                     /locus_tag="SCM56_002583"
     CDS             complement(3562..4056)
                     /locus_tag="SCM56_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HEG1111318.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4076..5296)
                     /locus_tag="SCM56_002584"
     CDS             complement(4076..5296)
                     /locus_tag="SCM56_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1111319.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5443..7278
                     /gene="lepA"
                     /locus_tag="SCM56_002585"
     CDS             5443..7278
                     /gene="lepA"
                     /locus_tag="SCM56_002585"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEG1111320.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
BASE COUNT         2427 a         1401 c         1507 g         2016 t
ORIGIN      
        1 aagatttata taagtcgaga aaaaaagtca aaaaactatg tgtatatttt gaccaaaatt
       61 gaccaaagcg atataatagt ttttgtaaag atcaaagaaa taagtgatct tacctttaat
      121 taggagggta tacctgtatg aatattgaga aaatgactac gacgcttcaa gaagcgattg
      181 ctgaagctca acagattgcg gtcactcgaa aacatcaaga tatcgacatt gcccatgtat
      241 ggaaaatctt tttacaatct aatcattttg gtcgaaattt ctatacggat gccggattgg
      301 atgtcgaatc ttttgaacat gaaattgatc gtctactgga tgagtatcca gttgtttctg
      361 gtggaaacgt ccaatatgga caaaacctca gccagaattt ctttcgatta ttgaatgaag
      421 cagaccaaat acgtgaaagc ttcggcgacg agtttctttc aacggaagtc gttattttag
      481 cgttgatgaa attaaaaaat tatccgttaa ctgtttattt gaataaaaac ggattatctg
      541 aaaaagaatt gcgtaaaaac atcgaagaga tgagaggagg agaaagagtg acctcacaaa
      601 accaagaaga acaatacaaa gcattagaaa aatacggcgt tgatttagta cagcaagtca
      661 aaagcggaaa aatggatcca atcatcggtc gtgatgaaga aatccgtgat gtcatccgta
      721 ttctttcacg aaaaacaaaa aataatcctg ttctgatagg agaacccggt gtagggaaaa
      781 cggcaatcgt tgaagggctt gctcaacgta tcgtacgcaa agatgtgcct gaaaatttaa
      841 aagataaaac gattttttca ttagatatgg gtgcattgat cgctggagca aaattccgtg
      901 gtgaatttga agaaagacta aaagcagtct tgaaagaagt caaaaaaagt gatggacgta
      961 tcctcttgtt catcgatgaa atccacaata tcgtaggggc aggaaaaaca gaaggaagta
     1021 tggatgccgg aaatctgttg aaaccaatgc ttgctcgcgg tgaattacat ttgatcggtg
     1081 ccacaacttt agatgagtac cgtcaatata tggaaaaaga caaagcgttg gaacgtcgct
     1141 ttcaaaaggt attagtcaaa gaaccaacag tggaagatac gatcagtatt ctgcgtggat
     1201 taaaagaacg ttttgagatc caccatggtg ttaacatcca tgacaacgca ttagtagcag
     1261 cagctacttt atctgaccgt tatattactg atcggttctt accggacaaa gcaatcgatt
     1321 tgatcgatga agccagtgca acgattcggg tagaaatgaa ctctatgcca acggaattag
     1381 atcaagttac tagacgtttg atgcagttgg aaatcgaaga agctgcactg aaaaaagaat
     1441 cagatgatgc aagtaaaaaa cgcttgaaaa atttgcaaga agaattagct gaacttcgtg
     1501 aagaagcaaa tgcgatgaag atgcagtggg aaacagaaaa agaagaagtc aattctgttt
     1561 cagcaaaacg tgctgaaatc gataaagcaa aacacgaatt agaagatgca gaaaataact
     1621 atgatttaga acgcgctgct gtattgcgtc atggtacgat acctcaatta gaaaaagaat
     1681 tgaaggaatt agaagctaaa gcaaaagaca gcgagattaa gatggttcaa gagtccgtta
     1741 ccgaaaatga gattgctcaa gtcgttggca gattaacggg tatccccgta acgaaacttg
     1801 tagaaggtga acgagaaaaa ctaattaaat tgaatgaaac acttcataaa cgagtgattg
     1861 gtcaagatga agcagttgat gcggtcagtg acgcggtaat tcgttcacgg gctggattgc
     1921 aagacccaaa tcgtcctcta ggttctttcc ttttcctagg accaactggt gtcggaaaaa
     1981 ccgaattagc gaaagcgtta gctgagaact tgtttgactc agaagatcat atggtacgga
     2041 tcgatatgag tgaatatatg gaaaaacatg ctgtgtctcg tctagtcgga gctcctccag
     2101 gctatgtagg ttacgaagaa ggtggtcaat taacggaagc tgtcagacga aatccttata
     2161 caatcgtctt gttggatgaa atcgaaaaag cgcatcccga tgtcttcaat atcttgttac
     2221 aagtcctaga tgacggacgc ttgactgatt caaaagggcg agtagtagat ttcaaaaata
     2281 cggttctgat catgaccagc aatatcggtt cacagttact gctggaaggc gtgactgctg
     2341 atggaacgat tccagaagca gtggctgaac aagtcaacac gctattacgt ggaaacttca
     2401 aaccagaatt cttgaaccga atcgacgaca cgattttatt tacaccattg agcctagaca
     2461 acgtgaaagg aatcgttgac aaaatggtgg cgcaattggc acaacgtctg gaacatcaag
     2521 aaatcctgct gacaatcagt gatgaagcca aaacgtggat tgctgaaaat gcttatgaac
     2581 cagcatatgg tgcaagacca ttgaaacgat ttatcactaa ggaagtagaa acaccactag
     2641 ctaaagaaat cgttgcagga catgttatgc ctaaatcaaa agtaacgatt actttacttg
     2701 atggacaact tcatttcaaa acagaagagc tagaagaaat cgtctaatct tccattgaga
     2761 ctataaaaga aaaaaggggt ctgacacaac tagtgtcaga cctccttttt tcaaataaac
     2821 agtgttccag aagtagtttc ttcgaaaaat aagccgaaat ttcttgaagc tgcccgcttc
     2881 tgtcacaacc tcttttatta ggtatttttc actttttcta atttataact ttcaaagtaa
     2941 cgctcatctt ttttactttg agaccatgca aacaatccca ttaaggcagc acctatcgta
     3001 tttgtcagca agtcgcccat cgtatccatc aatgctgctc gtccgacaaa cagtgtgcca
     3061 tcagaagcag caaaacgttg aagattcatg cctaagaact ggtcgcataa gaattcccag
     3121 aactcccaaa acacgccgca aagtccagca aacgcaaagc cgaataagag gaacaaccaa
     3181 ggactggttt ttgatatctc agcatctttc atcaagtaac caatcaatcc atagccaaga
     3241 gctgtcagga ccattggact caccgcatgt aagattttgt cccaaaaaga gatgatactg
     3301 atcaaatgca tccctgtgcc taaaaagaca gagatgaaca agaaaaacca gtaaaacaaa
     3361 acgattgctg ctggtatttt aagtttgaaa actttcaaaa tgacttgtgg catgaaaatc
     3421 aatgcaatcc ctaataaaga ttccacgata aatgtgattc cttgagaagt cgaatatttt
     3481 tgtgtgataa attcataaaa attatagcag acaacgaata caccaaaagc aagtaaagct
     3541 atttgcacct ttttgatttc tttaatcact gaaagtccct ccatctaaaa atctttttac
     3601 ttctgaaagc aactcaattt ctgaagaaac cacttttccg ttcaatttga tcaaacctat
     3661 tgtatataag ttcaggtagt gaaattgatt ttcagctgtt tcttgtagtg cagcgatttt
     3721 tttcgggtgg gttccgcctt gctgtcgagt atcagtatat aagccgatca ctgggatgcc
     3781 tttcgcataa gccacaccga tttccgatgc taccccggca tcgatcgtca gcccatctag
     3841 taaagcaatc attaaatcac tagccaacac tttttctgtg tcagcaagag caatcattcg
     3901 actgtctgca taagcagttt tgtcattgat tgcttcattt tcttgaggaa gatagatcga
     3961 aagctcagaa gagagctctc tgatttcttt aactaactgt tggttatata acaaatcact
     4021 tttagcaaac attggtgcgg caaaataaat tttcatctaa acatcatcct ttttatcaaa
     4081 aataatcgct aaagtaagct tccatgtttg gaatgatacg ctctagtgac ttcagtgttt
     4141 cttcatctgt ttctaaccgt tcgattcttg ccaaataaga agcacggcga taattcatca
     4201 tcaagcaagt caatgtttgg atatccagtc gaacggattt tcctataggt tcatttaaga
     4261 cacggacttg tccctgttca tcccatgtta aagcgaagac accattattc caaggagcga
     4321 caggatcttc aatgatgaaa tgaaaagcat cagctgttcc aacaaacgga aaccgttgaa
     4381 ggaattcttt tacatcaacg atccgagcca taaaatatgg ttcgatctgc tctttgatct
     4441 gactatcttc aatcaaaaat gctaaaggct catttttata aatatctcca tgtacccagt
     4501 agatcatgga aaaatgtgcg gaaataaagt tccataagcc atttcgagct tcttgatcaa
     4561 gatagaacat ttcttttacg tgaaatattt cttcagctac ccagtagaac agaacacctc
     4621 ttggttcatg gttgctatcg tagtagacag cggctgtgcg ctcatcttca ttttcgaaac
     4681 gccaatactc ttcccaatga aaatcactgc ggaataaagc accatggttt tgttgggcaa
     4741 atttgtcata aacggaaaat acgtctggat gatcaactgg taatcgctca accataccac
     4801 ttactgggat ttgtttaggt agctgtgtat ctctgatttt aaaggaaagt ttatcagaca
     4861 tgatttccca tcctttacgc cgatagtagg ggatactgta aggatacaaa taagagatcc
     4921 attgcttatt cttgcgcata ttatccaatg caacgataat tagatcttgc atcaatccat
     4981 ggttagcata ttctggataa gttccaactc cagtcacacc acccatttta taacgtgcac
     5041 cgtgaatatt gacttcacaa ggatagatag cgatttgaga gatcagttta ttttcgtgga
     5101 accatccgaa cactttcgat acttccaaaa tcggtttttt ggaacggata aaagctcgtt
     5161 tattctcaaa cccgctttcc tcgatgtctg cttcagtcac ttggaacaca taggaaagca
     5221 attcattgaa ttgatcgata tgttcttgag tgaccggttt taattcaaga ttttctctaa
     5281 aatctttttg atccattttt tcatctccat ttattagctt gataacagta tagcaaaatt
     5341 ctacaaaaaa aacttctata gctgactaat taaaaactct ttaaattact gcttttcgtt
     5401 ggtataatgg aaagtgctga ttatatgaaa agaggagacg ctatggatat aaagaaaatg
     5461 aaagaacgcc aagagcagat tcgtaatttt tcgatcatcg ctcatatcga ccatgggaaa
     5521 tctacattgg ccgatcgtat tttagaaatg acgcacactg tgacgtctcg cgaaatgcaa
     5581 gatcagctgt tagactcgat ggatttggaa cgtgaacgtg gaatcacgat taaattaaat
     5641 gctgtagaac tcaattacac agcgaaaaat ggagaaacat atatctttca cttgattgac
     5701 accccagggc acgtcgactt cacctatgaa gtctctcgaa gccttgctgc ctgtgagggt
     5761 gccgttttag tggtcgatgc agcacaagga atcgaagcac aaacattggc caatgtttat
     5821 ttagcgttag acaatgattt ggagatcctg ccagtcatca ataaaatcga cctacccgca
     5881 gcagatcccg aacgtgtccg tcaagaaatc gaagacgtaa tcggaatcga tgctagtgaa
     5941 gctgtattag ccagtgccaa atcagggatt gggattgaag atattctcga gcagatcgtg
     6001 gaatatgttc ctgcaccaag tggagactta gatgcaccgc taaaagcttt gatctttgac
     6061 tctatttatg atagttaccg cggtgttgtt ctaaatgtcc ggatcacgga cggtatggtc
     6121 aaacccggag acaaaatcaa actgatgagt aatggcaaaa catttgatgt tacagaggtc
     6181 ggggtcttct cgccaaaagc agttgctaga gactttttga tggtcggtga tgtcggttat
     6241 atcacagcaa gtatcaaaac tgtccaagat acacgagtag gggatacagt aacgttagca
     6301 gacaatcctg ccgctgaagc tttaccaggt taccgaaaaa tgaatccgat ggtttattgt
     6361 ggtctttatc caattgatac ttctcgttac aacgatttgc gggaagcttt agaaaaactg
     6421 caattgaatg atgctgcttt acaattcgaa ccagaaacct ctcaagcatt aggttttggg
     6481 ttccgctgtg gattcctcgg acttcttcat atggatgtcg tccaagaacg cttggaacgg
     6541 gaattcaatc tagaattgat cacgactgca ccatccgtta tttaccatgt taataaaacg
     6601 gatggtacga ctgctacagt agataacccg gcagattttc ctgaacctgt aacgattcag
     6661 gatgtagaag aaccattcgt caaagcacag atcatggtcc caaatgattt tgtcggtgca
     6721 gtaatggaac tttctcaacg aaaacgggga gaattcatta cgatggatta tttagatgat
     6781 taccgagtaa atgtcgtcta taatattccg ctttctgaaa ttgtctttga ctttttcgac
     6841 aaattgaaat caagcacgaa aggctatgcc tcattggatt atgaaatgtc tggttatcaa
     6901 aaaagcaagt tagtgaagat ggacatcttg ctgaatggag aaaaagtcga tgcattaagc
     6961 tttatcgttc accgagactt tgcctatgaa cgtggaaaag caatcgttga aaaactgaaa
     7021 aaactaatcc cacgccaaca atttgaggtg ccaatccaag cagctattgg acaaaaaatc
     7081 gttgcccgtt cagacatcaa agccttgcgt aaaaacgtct tggctaaatg ttatggtgga
     7141 gatgtttctc gtaaacgtaa actcttagag aaacaaaaag aagggaagaa gcggatgaaa
     7201 caaatcggct ccgtcgaagt tccgcaagaa gccttcatgg ccgttctgaa aatggacgag
     7261 gacgaaccga agaaatagca agggtttgtt tgaaaacagt gtaaaaaata aaccatgttt
     7321 acatggattt gccaccggtt tgccaccggt g
//
LOCUS       DARBDV010000117         6886 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000117 DARBDV010000000
VERSION     DARBDV010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.120"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..182)
                     /locus_tag="SCM56_002586"
     CDS             complement(<1..182)
                     /locus_tag="SCM56_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG1111321.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="SCM56_002587"
     CDS             517..951
                     /locus_tag="SCM56_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEG1111322.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="SCM56_002588"
     CDS             974..1294
                     /locus_tag="SCM56_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111323.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="SCM56_002589"
     CDS             1435..1626
                     /locus_tag="SCM56_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111324.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="SCM56_002590"
     CDS             complement(1697..1954)
                     /locus_tag="SCM56_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HEG1111325.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="SCM56_002591"
     CDS             2112..2375
                     /locus_tag="SCM56_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111326.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="SCM56_002592"
     CDS             2399..2587
                     /locus_tag="SCM56_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111327.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="SCM56_002593"
     CDS             complement(2670..2855)
                     /locus_tag="SCM56_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111328.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="SCM56_002594"
     CDS             3070..3441
                     /locus_tag="SCM56_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEG1111329.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="SCM56_002595"
     tRNA            complement(3591..3663)
                     /locus_tag="SCM56_002595"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="SCM56_002596"
     CDS             complement(3709..4137)
                     /locus_tag="SCM56_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111330.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="SCM56_002597"
     CDS             complement(4197..4955)
                     /locus_tag="SCM56_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEG1111331.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            5250..5447
                     /locus_tag="SCM56_002598"
     CDS             5250..5447
                     /locus_tag="SCM56_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111332.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            5553..6035
                     /locus_tag="SCM56_002599"
     CDS             5553..6035
                     /locus_tag="SCM56_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111333.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(6093..6689)
                     /locus_tag="SCM56_002600"
     CDS             complement(6093..6689)
                     /locus_tag="SCM56_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEG1111334.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT         2331 a         1059 c         1353 g         2143 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tattatattt taatatgtag
     5041 gaaataatta gtttttaagg aatatgtttt aattttttga aaaaaattct tctaaataca
     5101 acagctgttt taattgtgaa aacgaaaaca gtacgctata atgcctgtaa ggttgatttt
     5161 taactgtaaa taaaaatcaa tgagttaaat ctccaaagaa atagcagtgg taatgtggag
     5221 aaaagttcca gcagtttagg aggattttga tgacggaaga aatgattaat ttaggagaac
     5281 aatatgcatg caaaccaatt ggttttacaa aaacggtcat tggtgaagtt gtatcaaaaa
     5341 tgacaaactg cgccgtggtc aaagttgccc aatgtgctgc agaggaccaa gaattattag
     5401 atgaaaaagc aagcatggtt gttgcaaaat atgatacatt tgaataaaga aaacaattgg
     5461 acgtgcggcg tgctgcaagt ccaatttctt tgagaaggta aatcagtatt cttttcctat
     5521 tttctggaga agaaaaagcg aggcgagttg tcatggtaac attctttacg gttctattgt
     5581 ttttattcgt tgttgtgatg tttttatttc tttggctggt tcgaaaagaa attatttatc
     5641 ggactgtgcg aaatcgatgg gtctacttgg tgataccctt cttagtgtca ctagtcattt
     5701 ggtatacact catcagtcat cctacagcag atgaattggc aaaagggatc ttgtcagcaa
     5761 tgatttttat tagttttttg ttggacagta gaggaatcac agaagaagga cttgttttga
     5821 atagctttga caaaaaaggt gttccttttt ctgagatcaa taaaattgta ttatatcagc
     5881 caaaaggttc taaaatcgta aaaatgaatt ttttccgaaa cggatggcga ggaccgatgc
     5941 tgaaattttc tgcatcatta gaagaattag ttccattttt gtcccaacgc ttaaacgatg
     6001 aggcagagat tgatattatg attgatccag aataaaaaca gaggttgcga caagatgttt
     6061 gtcacaacct ctgtttttta tttcaaataa aattatccaa atacgcggaa gcgatcggca
     6121 tcgttcatgt attctttttc cccagccggt gtttctggtg aaccgaatac taattgcgaa
     6181 cgtagtttcc agtttgaagg gatattccat tcttttgcaa ctgcttcgtc aataactggg
     6241 ttgtaatgtt gcaagtttgc gcccaatcct ttatcaacta atgctaccca agtattagct
     6301 gttgcgattc cgtttgattg ttctgaccaa tctgggaagt tgtctgcata taattcaaat
     6361 tgttcttgta gaccttttac cacgtcagta tctttgaaga ataatactgt accataacca
     6421 tttttgaaac cagctaattt gttttgtgta tttgggaaag cttctgctgg agtcaatggg
     6481 cgaagtgctt cttcagtgat ttcccaaagt ttttcatgtg catctccgaa aaggataact
     6541 gcgcgtgttg attgagcatt gaaagcagtt gggctttctt taattgcttc tttgatcaaa
     6601 gttgttaatt cttcattaga aagtgaaaca ttacgtccta gatgatagat tgaacggcgg
     6661 tttttcaaag tgtcagtaaa agttgtcata ataaaatttc tcctttgatt tgcgttttgc
     6721 atttttttaa ttgattacga aatgaagtat agcaccttac aaaaagtaag taaacagata
     6781 tgctcacaaa aaataagcga agttcaaaaa acaattttaa taagaaaaat cgttatgtaa
     6841 ggattttctt agttatcgct ttcattgttg tgttttagga attttt
//
LOCUS       DARBDV010000118         6696 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000118 DARBDV010000000
VERSION     DARBDV010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.121"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(7..1164)
                     /locus_tag="SCM56_002601"
     CDS             complement(7..1164)
                     /locus_tag="SCM56_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1111335.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(1167..1523)
                     /locus_tag="SCM56_002602"
     CDS             complement(1167..1523)
                     /locus_tag="SCM56_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111336.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(1821..2795)
                     /gene="sgrA"
                     /locus_tag="SCM56_002603"
     CDS             complement(1821..2795)
                     /gene="sgrA"
                     /locus_tag="SCM56_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HEG1111337.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(2991..3830)
                     /locus_tag="SCM56_002604"
     CDS             complement(2991..3830)
                     /locus_tag="SCM56_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEG1111338.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            4015..4902
                     /locus_tag="SCM56_002605"
     CDS             4015..4902
                     /locus_tag="SCM56_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEG1111339.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(5486..6181)
                     /locus_tag="SCM56_002606"
     CDS             complement(5486..6181)
                     /locus_tag="SCM56_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEG1111340.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(6165..6563)
                     /locus_tag="SCM56_002607"
     CDS             complement(6165..6563)
                     /locus_tag="SCM56_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HEG1111341.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         2105 a         1319 c          983 g         2289 t
ORIGIN      
        1 cttttcttaa ttaacgaaat ccaatttttc caatatggat ctatttttct cattcctgat
       61 ttttatgatt tcagctttca gacgcgccca atcatttttt gtaggttgtc cattccagta
      121 aaatatctgt gtattcgttt gatcatatgc ctttttgtcg ataggataat cgctaatgac
      181 aatatcaggt aactgatcag cggatgcact atattttatt gggcgttcag tcatagataa
      241 tatccttttc tttgcccata attcctgtgt tttaccatac tttgattgaa tatagaatat
      301 gacaggttgt tcgtgtttta taaaaatatc cctgactaac atacagtatt gaaaaatcaa
      361 ccgtctgttc ttttcataat aagtattaag ctctggaatt ctgcgagcta ccccactaaa
      421 aaactgttgc atgattggat acatcactgg aaatatatgc tgaaaatctc tgtcttctgc
      481 tgattttcca aaagcatcca ccttcttttt tgtcccaaaa acccttgaat acaaatggat
      541 cattgccaaa ttgacatata gataattttt ttctgaagtt gtaaaacaaa tcttgaagtt
      601 tttttcaaaa ttttctacaa tagacgattc aatccagccc caactttctt gtactttatc
      661 tggagtatcc aacaaatctt cttccaaata ggtgttcatt acaccaaaca taaaataaag
      721 gtaagcttgc tctgttggag tgatgtgatt catcgaccag aagtttgctt caaaaaaagg
      781 ctctagtatg taatgcgtaa actcttcttt tgttatcaat ggatgcacaa atttgttcat
      841 ctcttctggg acatacgtaa tcgagtggcc ttgtttcatt cgttgcaacg aaattcttag
      901 taggctgatc catttctttc tatctatact acgcgtatat ggcgcatgtt tcgcaaattt
      961 ttctaatagt tgacgtttat tttttatact tgataatata tcagaatgat tttcgtcgaa
     1021 tgactgccaa tacatcaagt gaaaaaagta tcggatacgg tattcttctc cttctatcgg
     1081 attacctttt ttcaagctgc aagataaatg atagtttttc aaaatagtat taagttctct
     1141 taattttctt tgaatcgtac gcaaacttat ataatttttt gactaagatt ttctaaggat
     1201 tgataagtac ctttgtaaag tgaatccagt aaaatataac caatcgttcc ctctaaaagt
     1261 atagaataaa ctttactcag taaaaactga gaagaaaact cgacttttat catcttgttt
     1321 tctgaaagaa aagataattt atattctgga tacctttcgc aaaacgattc tgcttgttga
     1381 atcaccgaat tcatcttagc cagggtgtag tttagatcta aagcgatatc cttcaccaca
     1441 tataatttct cgtctctttc ataacaatag ttcaaaaatt gatatacttg cctttcattg
     1501 atatcaataa attcttcaaa caaagtacat actccttttt atctattatt tagacttatt
     1561 ccgacagcta catgaataac gtacaaatag attcgttact gatttttcag agatactacc
     1621 atgcgcagtg tgcattgtca attattcttg ctcttatttt ccatttgtgt tcctgttatt
     1681 tcctgtttct caaatatgtt agtaaacaaa aatagatctg gcaaagctac aacttcgcca
     1741 ggcaatacag cactctcgaa tgaaatattc actcaaaaat ctatcgtttt attcaatttt
     1801 agatctgttg cttttattct ttatttcact cgatggaaga gagaacgaaa atagcttgct
     1861 cctacaatca aaatgagtaa cacacccatc catgtaatta gcttgttcaa ggttctacta
     1921 ccagtttctg gtaaatcgct ttttctatta tctgtttctt tcgctttttc ttgatgatca
     1981 attccacgag aaacaaaatc aggctgtttt agcacacttt ttaggttgct ccctgcattt
     2041 ctcttttgtt ccttagttgg tatgataggt accaaatcaa gttcattatt tacttttcct
     2101 gtgctgcttt cgctgctcgt gcttcctgtg ctactctcac tactcatgct tcctgtgcta
     2161 ctttcactac tcgtactctc cgtactgctt tcgctgctcg tgcttcctgt gctactttca
     2221 ctactcgtac tctccgtact gctttcgctg ctcgtgtttt ctgtgctact ttcactgctc
     2281 gtactctctg tactgctttc accgctcgtg ttttctgtac tgctttcact acttgtgcta
     2341 tccgtactgc ttggcggtcc aacaactgtc caactgcttg gtttcaaatt ctccgtgatc
     2401 gtcatctgtc catcataaaa aacaatcaaa tcaactttat ctttactctt gatctcatca
     2461 atcgatagtt ctgcagaaat cattattgta tactcaatac cagcggtcca ttgtacgtct
     2521 tttaaataaa cttccccaat aactttttct gaacttccat taccatccca accatatgtt
     2581 agaacagcat gtacatcact agcattcacg cttttgtttg gtttgactgg aatagtaatg
     2641 acttgttgtg tcatgtcata aacaatccga tcagttttca cagtaaatga aagattctca
     2701 tttgcccgtt catttgcaga agtactgatt ccaatcattg ctgcaattat actcagccca
     2761 agaataacaa ataatacggt cgctgttttc ttcatttcta tcactctcca tataattacc
     2821 ttctatttga taaaggaatt atataaaatg aaaatgatga gaacacgaca gtcagttgtg
     2881 aaaatctaac aaaagatgtc gtgtattcgc ttgaatttgt ttactttttc gtttaatcaa
     2941 tgacagactt aaaaaagcaa gaccgatatc agccttgctt tcttcatctt ttattgataa
     3001 acagcagtgt atttcttcat ccgctttgtt ttatatgcat agagtaacaa accaacaatc
     3061 aagactaaca agaataatgt agttgatata gatgcttcag ccccaaagtt tcccccagta
     3121 attattgtat tttgcgattg gatcagagtg gagaaaaaac cttgttcttg cgttccgctg
     3181 actggtatac ccaaaatagc tccttgtgtc caattccaag taatatgagc accaatagcc
     3241 gtaaataaac ttcctgtact gtagataagt gcgctgaaaa agcaacctgc taaaaagata
     3301 ttgaaaactg ccagaaatgt catgttagga ttcaatccgt gcaaaagagc aaataatgca
     3361 gcagatccaa tcattgctat taacggacca aatctaagca tctgatgctg aaaaaagcca
     3421 cggaataaaa gctcttctga tgcccccact gcaataaata aaataaaact cgttatcagc
     3481 ggcacaatgt ttgcgtttcc tgaatctaac cgatacgccc ccgtcaaata taatggacta
     3541 gtaataagca gaaacactcc aattccaaca acgagacctc ccgctaaata cccccagtct
     3601 tttcctcgaa cagaaacacc ataatcttct attctttttc cttcgacctt actagcaaga
     3661 aataaagcta gtaaaaaaac agctaaatgt ccgaccgtac cggcaattgc tccaatccat
     3721 tcactatttc caataaacat atccgcaagt gcattattga caagaattcc taaaacaaaa
     3781 ccaactaaga ctattaaaac agatccaatc aaccgaataa tcctgatcat ctttcatccc
     3841 tcctttatat taagtttaac aacccttttc tttcttgtct cgtgaaaact atgtaaatgt
     3901 tctccatcat agagagaata gatttttacg aactaagaat atctacagca accttacgaa
     3961 aatgaaagac tgtctcccta attcttctgt tagactgttt ttaataagga aaggatggga
     4021 aattttttga aaaaacaact actaattatg tgcctttcag catgtgcaat tttactattc
     4081 tcaggttgta gcaagtccaa tgaaacgatt gcaacgttta aaggtggaac acttacagtt
     4141 caggattttt atgataaccc aaaagttaaa tcaaataatc aaacactatt aaaaaaaatg
     4201 attgtcttca aagcatttga tgatgtatac ggaaaagaga tttcttcaaa ggaagtaaca
     4261 aaagaatata atgaacaaat caaaaaactc ggtccaaact acaaggaaca attaaaagca
     4321 gttggccaaa cagaagagac atataagaaa ctctttaaac agatgctggc tttccaatac
     4381 ggtttgaaag ccaatgtaaa attaacagat aaagatctag acactgcttg gaaagaattc
     4441 tatccagaag ttagtacaca gatcattcta ttttcgacta aagaagaagc agataaagca
     4501 aaaaaagaag caaatgaagg ggaaaatttc tccaagcttg ttcaagctta tggaaaaaat
     4561 aaaacattaa aggaaactga tggaaaaatg aattttgatt caaccaatcc agagattcct
     4621 actgaagtaa aaaaagcagc atttaaacta aaaaacgggg aagtttctga tatcattcct
     4681 gtaacagatc caacgactta tcagcaaagc tattatctag taaaaatggt aaaaaaacag
     4741 gataaaggtt ctaataaaga caaatacaaa tctgagctag aaaaaattgc taccgaagca
     4801 aaaactcttg atacggaatt catggataaa acaatccgta aagtgatgaa aaaagacaat
     4861 gtaactatta aagatcctta tgtaaaaaat atattcaaat aaacaaattt gacccaattg
     4921 gggatgtggc aaaatttgag aagtgatttt tggaaattcc ttcttatttt tgaggcaaac
     4981 cacttttgcc acaacttcat cggggccttt ttttatttgt accaaaacaa atcactggat
     5041 ttgttacaat catgaggata taatttaaaa aagtttttta tttctatcac atgaatcaat
     5101 gcgccattaa gtagattctt tcatgcttcc ctattttttc acttgccgta tcgcttcaaa
     5161 aacaactatc ataaactcaa actcgactga aaagttattt taaaaaagac taaaaatatc
     5221 ccttctggac ttgttcctct tattctgtta tactagagaa acacgaaaga tatttttatg
     5281 cgacactttt catttgtttt tatgaaattt attcaaaact ggcgtaactt cagagttaca
     5341 aggatgtatt agaggttagg gaatacatcg tttaaatgag aaataagtaa tagtcaccca
     5401 aactttaaat taatgaatat tgaatcgtca gatagcctcc tcgagttttc gagaaggcta
     5461 tcgtttattt tatggagaaa ttgattcaga agtaatcagc gacctttttt agttcaccaa
     5521 tcgtttctgc ttggaaagtt tgcacccaat cttcttggaa gcccctcact gctttatcaa
     5581 tcgagggcat ctctaatact tgatctagta gctctttacc taacgttatc gcatgagctc
     5641 cagatcgaat ggctgtagtg acttgttcac aatttttgaa actagctgct aatatttttg
     5701 ttggtctttg cgtacgttcg atctcagtcc gcaacgaatc aatcactttc atagcatcaa
     5761 tgccttgatt ttgcatcctg ttataataag gtgcaatata atctgctcca ttattaatag
     5821 ctaaataccc ttgtaattct gtgtaaatag ctgtaccggt aatcaaataa ccttcttttt
     5881 tcaacttttt gatcgcttga aggcctgctt gattcaccgg caccttgata aacgtatctt
     5941 ttcccagttc ttctatgata actcgagcat cctctatcat ttcttcttcc gttcttccta
     6001 ttgtctgaat atggatagga atctgtccga ttattttttt gatttctttc atatgcgaga
     6061 agaagtttga cgctccttct tttttcagaa ttgtaggatt tgttgttacg cctgttaaat
     6121 tcaaaaactg ttgacaagaa taaatacttt ttgtatttgc tgtatcaagc atgatttcca
     6181 tatagctttc ccccattctg atcctcttga tcgattttaa tgattgtccc ctttcttaaa
     6241 tgcggcattt ccttatcttc tacatacatt gttcctggaa gttccggctc atgagatcca
     6301 tcaaatttta tcgtgatatg tcctaattca ttcaaattag tttggacaac atctcctact
     6361 gctgtgatta catacatttg tttatcaaaa cagagcttca tatcacaagt tatttctttt
     6421 cttacctgat tcaattcaat cttataacaa taatctgtta atgtttccgg tgcttcatct
     6481 ccaaataaaa tcattatttt ttctttcctg aatacttctg cttctatacc aacatctgtt
     6541 acaaccgttt caaagatcgt catctctgtt cctcctcgta aaatatcatt tgtctaatag
     6601 tttttattca aaatgtttct agattataaa atttattgaa taacacattc ctaacggaat
     6661 ttgtttcgat attgtgtgat ttctcagagt acaatg
//
LOCUS       DARBDV010000119         6644 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000119 DARBDV010000000
VERSION     DARBDV010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.122"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(1..859)
                     /locus_tag="SCM56_002608"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="SCM56_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="SCM56_002609"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="SCM56_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEG1111342.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="SCM56_002610"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="SCM56_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2603..3421)
                     /locus_tag="SCM56_002611"
     CDS             complement(2603..3421)
                     /locus_tag="SCM56_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG1111343.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="SCM56_002612"
     CDS             3582..4271
                     /locus_tag="SCM56_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEG1111344.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="SCM56_002613"
     CDS             4285..5787
                     /locus_tag="SCM56_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1111345.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="SCM56_002614"
     CDS             5800..6276
                     /locus_tag="SCM56_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1111346.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="SCM56_002615"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="SCM56_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1204 g         2236 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DARBDV010000120         6570 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000120 DARBDV010000000
VERSION     DARBDV010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.123"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            127..579
                     /locus_tag="SCM56_002616"
     CDS             127..579
                     /locus_tag="SCM56_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111347.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="SCM56_002617"
     CDS             593..1282
                     /locus_tag="SCM56_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111348.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="SCM56_002618"
     CDS             1407..3758
                     /locus_tag="SCM56_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HEG1111349.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="SCM56_002619"
     CDS             3838..4233
                     /locus_tag="SCM56_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111350.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4558..5175
                     /locus_tag="SCM56_002620"
     CDS             4558..5175
                     /locus_tag="SCM56_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1111351.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5216..5515
                     /locus_tag="SCM56_002621"
     CDS             5216..5515
                     /locus_tag="SCM56_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HEG1111352.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5549..5716
                     /locus_tag="SCM56_002622"
     CDS             5549..5716
                     /locus_tag="SCM56_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEG1111353.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5761..6366)
                     /locus_tag="SCM56_002623"
     CDS             complement(5761..6366)
                     /locus_tag="SCM56_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1111354.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2265 a          910 c         1285 g         2110 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatttaatg gcagaccgtt aaggcctgcc atttttatgt attttagcgg
     4381 aataaatata atcatgcggg agtgtaaact tactgtttca aaaagtatac tttttgacat
     4441 gccttgattt tctttgttta tgtatggcat aataaccaca taatacaatt tgaaacgttt
     4501 ctgatttaat ctgattgttt tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg
     4561 agaaaatttt tttatatccg ggtcagtagt tatgatcaat cgatcgaacg acaactgatt
     4621 tccgcgaagg aattaggaat ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa
     4681 gattttaaac gccctgaata tcaattaatg aaacggatgt ttcgaaaagg agatttactc
     4741 tatctacaat cattagatag actaggcaga aataaacaaa tgattttaga agaatggcga
     4801 gaattaacta aggaaaagca gattgatatt tctgtgttag acatgcctct gttagatact
     4861 gctagttaca agcaactaga tgggttagag acgcttatct ctgacttaat tctgcaatta
     4921 ctcagctata tggccgaaga cgagcggaaa cgaattaata gccggcaaag agaaggcgta
     4981 gaagctgcga aacaaaaggg tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa
     5041 tttcctgcga tctattctga ctggaaagca ggaaagataa ctgcagtcta tgctatggat
     5101 caattgggca tgaaacgaaa taccttttat cgacgggtga aagaatatga acataccttg
     5161 caccactttt cttgatagat ataacagcta ttagacggga agggagagtt tgaaaatgga
     5221 cttaacgaat caaagggtcg tttttactgg aacgctgcag caaatgacac gaacccaagc
     5281 catcggtttg gttcactctt taaacggcca ttgtcaatca agtgttactt caaaaacaaa
     5341 tttcctggtt tgtggtcagt attctaagtc gttgtttgat gattctctct atacaaaaaa
     5401 ggaacaaacc gcgcgggact tgattgcgtt aggtcatgag attaccattt tatcagaagt
     5461 cgaattttat gcgttaatta gtcaacagtt gaaagaatgg caacggtatt tataatcgtt
     5521 agtaaaattt acaaggagga aaatttttat gaagcgaaaa tcttatgatc gttggggcat
     5581 tgaaataact agttttaatc gaaatattgc ttttcacgtg gagtgtgact gtggggaatt
     5641 agcaaaaagt gtttatcaaa aaaattactt ttactgtcct aaatgccatc gccgatacgg
     5701 ggtacatgga acatagaaac gaaatagtgc ggtagaaaag cgaatgaatg tagtatattt
     5761 ctacaaatca ttcgctttct ttattttata aatagttggt cgagagatcc cgttttcttt
     5821 tgcaatctct gcaactggaa tgccttgctt taacatgtcc accacggttt taaaaactaa
     5881 gcgtttttgc ggatctgttg agttaataga atactcgatt ggtcgtccct tatatcggcc
     5941 ggctttttga gccaataata ctccttgccg ctgacgttca cgaattttct cacgctcgtt
     6001 ttgtgcaata taacttagca gtgataaaaa catatcgaac atagcggtat ccaggagttc
     6061 atttccagta ttaaaatcta aaaactgtgc atctaatatt tttaacgcta cttttttctg
     6121 tttcataaaa gcaactgtat tttttatatc ttcataatct cttcctaaac gatctagtga
     6181 tgtaacgatg acgcaatcac cttcacggat ataatttagt aatttttgaa actcaggacg
     6241 ctcagtatct ttaccactgg ctttttcttg gaataattta tcaatgttaa gtccttccat
     6301 cattgccaat tgtcgatcca aattttgatc ttgtgaagat actcgaatat agccaatttt
     6361 tcccataaaa aactcctttt taaaagtgat ataatttaca tgtaaaattt acttgtagtt
     6421 ttactgctat catagcattt ttaaaaataa attttatgta gacttaaaat ttacttcatg
     6481 gagggtttaa atggatgaaa tcaaaagtaa caaaaaagca tttggcattt atttaggaga
     6541 acctagtaca gaacaattaa accaattttt
//
LOCUS       DARBDV010000121         6303 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000121 DARBDV010000000
VERSION     DARBDV010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.124"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            334..714
                     /locus_tag="SCM56_002624"
     CDS             334..714
                     /locus_tag="SCM56_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEG1111355.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            696..1610
                     /locus_tag="SCM56_002625"
     CDS             696..1610
                     /locus_tag="SCM56_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEG1111356.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            1855..2544
                     /locus_tag="SCM56_002626"
     CDS             1855..2544
                     /locus_tag="SCM56_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111357.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(2665..2751)
                     /locus_tag="SCM56_002627"
     CDS             complement(2665..2751)
                     /locus_tag="SCM56_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEG1111358.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            3427..4377
                     /locus_tag="SCM56_002628"
     CDS             3427..4377
                     /locus_tag="SCM56_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HEG1111359.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            4398..4931
                     /locus_tag="SCM56_002629"
     CDS             4398..4931
                     /locus_tag="SCM56_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEG1111360.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            4947..5117
                     /locus_tag="SCM56_002630"
     CDS             4947..5117
                     /locus_tag="SCM56_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111361.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            5567..6100
                     /locus_tag="SCM56_002631"
     CDS             5567..6100
                     /locus_tag="SCM56_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111362.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
BASE COUNT         2323 a         1102 c         1174 g         1704 t
ORIGIN      
        1 aaaaaaaaat aaatcgatac attttagaag aatagggttg tttgaatatt acaaagcaac
       61 aatttcacct tcggtgaaat aagcgaaact ttacaaaaag tggctagcaa atgctagcct
      121 ttttttgtat tgtggagcgt ggattttgag ggggattcaa gggggagctt gcgctccttt
      181 gacaagcggt tatttgcacc aaatacggag gtatttggct agcaaatata gagcttgcca
      241 aaccctttta gtgagcttta ctcattgtac ctttttaggt acaatggtat tgcgaacgat
      301 acgtccgtag gacgtactga aagggtggga cttatgagcg aacaacatca aaatttggct
      361 agcgatcatt ctaaaaaata tacctatcga tccgagccaa aacaaatcag ttttcgagtg
      421 agcgaatccg aatttgcaaa gctgaagcag tcagctgaag ctttgcaaat gagtgtgccg
      481 gctttcgtga aagccaaggc acaaaacgcg cgtctggtga caccgaaagt ggcgccagac
      541 attgcccagg caatcgcgcg cgatttagcc aaagctggag ggaatatcaa tcaaattgcc
      601 aagtggtgca atacacacca acacgacgtt gctcctgatg atgctcaacg tttatccgaa
      661 aacctaaaaa tcatgcaaaa ggagctacaa aagatatggc aacagttaaa gtaagtcgaa
      721 ccacttcatg tagccgagca atcaattacg cagaaccacg agcaaccgtc aaaacaggaa
      781 taaactgtga cattgattat gccaaaagtg aaatgaaaca aatacgcacg ctttatggaa
      841 aagacgatcg tgtacaagca cacttattga ttcagtcctt tcgaccagga gaaattaccg
      901 ctgaaaaagc caatcaacta ggggcagagt atgcgaaaaa aatagcccct aaccaccaaa
      961 tcgcgattta cacacataca gacaaagacc atattcataa tcacattgtc attaactccg
     1021 ttaatcttga aactgggaaa aaatttcaag cccatgggca agcattttta aacaaatgtt
     1081 atgacctaaa tgacgagatt tgtcttgaac atgacttaac aatcacggaa cgaggaaaga
     1141 aaaccgaaaa acgaaccatg tctgaaatta aattaaaaga aaaaaatgaa ccggtttgga
     1201 aagacgaaat tcggtttgcg attgatcaaa caatgaaaaa caaaaaaact cgaacgtata
     1261 cccaattttg tgattcactt aaaacattcg gcattcattg cttgaatcgc ggaaaaaatt
     1321 ttacttacga attggtccac aaaaagaaaa aagtacgatc caacaaacta ggaaaggatt
     1381 acgaaaagga gacgatttta catgagctgg acagacgaga acgaacctac aatcaacaac
     1441 gaaccaacga gctacgaaga gctcgagcta gcattcgcga aactgcagaa gaagtacgaa
     1501 gagaacaacg aacttttacg cctgaacaat cggaaattag accgactcga agaaaccatg
     1561 aacaacacgt tgaacgagga acagaccacc aacaagaatt atcgctctaa aacgttaaaa
     1621 gacatcgaaa acgaaataaa gagacaacgt gcctttcttg cgccttatac ccaagaaagg
     1681 atacaaacta cgatggattc acaagtcaaa aaactccacc aagcgctatt agaccagcga
     1741 aaccaagaac aaaaaaaggc tttaattcaa cgatcgatgg atattcttgc ccccttatgt
     1801 tgcttagtac taacattatt agccttttgg ttggtatggg gagaataaac agacatggca
     1861 ttctatttac cctatctact catttctgcc agtattttag gaattatttg gcttagctat
     1921 aaggcttatc aaacaagata ttcattaaaa ggctctaaaa ttcgatttaa gcgattttta
     1981 ttggtcgggg ttatatttgc cttatcaatc gttttaagtg gtttattcgg cgttttagag
     2041 ggtaataaac gtgtttcaag aagcatttta ttaggggata tcactcaaaa gtatgaagca
     2101 gcatgtgata aaaagaaaaa agagcaagca ttagcacaaa aaatggagga atttaccgct
     2161 tgttatgagg acatgaacaa tatttttgtg gatcaagaga aacgtttaac agataaaaac
     2221 atgaaaaaac tcactcgttt ttatcaaaac ttaccagaaa aacagcaaaa agaggtacaa
     2281 gaaaaatatg agcaagtaaa aaaagacgtg caatatttta aagatacgca gacagaagaa
     2341 gcttgttgta acttatttag cgatacaatc cctttttcaa ccagtgaaca agaacgaaaa
     2401 gaacggcaac aatcggtcac atacgaacgt tatgaaacac tgcttcagca agcaacaaac
     2461 atacaaaatc caacgaaaaa agaaacggca ttaaattatc tagaaagtgt caaggaatgg
     2521 ttggatcagc aacaacaaaa ttgaatttga ttagcaatca tgttaaactg aacctgcaaa
     2581 tgatcaaaca ttagatacac gaaagcccca gagaggtcgg attctctggg gctttttttc
     2641 aggttgatgt cgcctttatt gattttactt cttgtttcgg ttattcaacc aataactgaa
     2701 taaagagaca caaacttcta caagaagggg gcaaatgaac cgttcaaaca ttagatacac
     2761 ctccttccag tcgcaagact gtcggatagg cgacaagtta ttatacctta caaaataaaa
     2821 tctgtgtggt gtttttaagt atcccttatt ttcgggttgt tagggcttgc cctgaccgcc
     2881 tgtaaggcgc tttaagaaca agataaggtt ttttacccaa gactgtctta aaacgactta
     2941 gacgagctat cagaggcttc ccccacctcc gttactgact tgtttttagc aaaggagcct
     3001 ttaggcgact gcgcagtaca atgtgagagc atagcgatca cactttttta gcgaccgtag
     3061 ggagcaaggt tttgaattgg cagttttgaa aaatgcgcga acctgttggt agaaaaaaga
     3121 agtggttttt aggcgggaaa ttttgtcacc ctcttttata atctcttttt aaaccttttt
     3181 ttaaacctct ttaccgtcgg ctgtgtcctt actccttcaa tggatacagc gttttataaa
     3241 ctacattttg tctgctttta aactacattt tgtctgcttt taaactacat tttgtctgct
     3301 tttaaactac attttgtctg cttttaaact ctttttttgc actcacgtat gttataatat
     3361 gcattatcac agacaaaacg ttgtttataa cgtctgtatc cattgaagga gtaaggagga
     3421 atatctttgg caaatgaaat cgtgaaatac catcatgaat taaataccat tccgttgcgg
     3481 aaatttacac cagttgaaat gaatctattt ttctctattg tgtctagaat gcgcgaaaaa
     3541 ggcgataaaa cagtccgtct tacttttgac caactaaaag atttaagtaa ttataaagca
     3601 actgctaata atcgtttcat taatgattta gaaatgactt atgataaatt aatggattta
     3661 cgttttggta aaagaagtgc taatgggctc gatatagata gattcgttat gtttagtgag
     3721 tttaaaatac gtggaactgc taaagatcca tatgtagaca tacaagttta cgaaaaagct
     3781 ttacctttac taaacaattt agatgagtgg gttcgctatt cactccaaca atttaacgaa
     3841 ttacaaagta gctatgcaaa gactatgttt cgattactaa aacaatatcg aactaaaggt
     3901 tttgcttatt tttctaaaga ggattttcat gaactattag atattccaaa aagttactgg
     3961 aacaaccaag caaacgttga taatcgtgtt ataaagccta tacgacagga attaaccgca
     4021 atcttcaaag gattatcaat caaaaagaaa tacggaaaag ggcgaggaaa acctgtcgtt
     4081 ggctatcaat ttacgtttaa accagaaatc aaaaaagcag atgattttaa taaacatgca
     4141 ccaaccaaag aagaacaaaa taaagcattg gctttagctg ataaacaaaa agaatacgaa
     4201 gaacctgcta caacaattaa cgaaaaaatc aaacagaaga aacgagaact tgaactcctc
     4261 caacaaatca aacaactaga aaaagagaaa gaagctcttg aaaaacaaac agagtccgca
     4321 ctttcgaaaa aagagaaaaa aagtttatta tctgatttag ataatttatt ccagtagcaa
     4381 cctaggaggc aaccagtatg caaccacaag aaacctttac aataaaggaa ttgtcagaac
     4441 tttttaagat gagtaggcaa gcaatatcga aacatatcca aaaattagat tcttccatga
     4501 tagctaaaaa tgagcgtggt tataaggttg tattgcgttc tggagtattg caacttgctc
     4561 gcaacttagg caaccaaatg ttgctagata ggttgcacga gtacgaacaa gacaacgata
     4621 aaaatgttaa tataccaagc aaaaacgtgg ttacaaacaa gttgctagat cagttgcgtg
     4681 aagaaaatga atttttacga gaacaacttg ccctcagaga gcaaaataat tctcatatac
     4741 aaaaactact cgaccagcaa caacaactca ccctacaagc caacaaacaa atcgaaaaac
     4801 tgcaagaaaa actacagttg acttatacag aagaagctcc agagaatgac tcaacgactc
     4861 tttctgaagc aacagaaaca atagacaaac aaattgatac tgaaaaaaaa tggtggcaac
     4921 tttggaaata actggaggga acattcatga caagaaaaaa tcttctattc ggttctattt
     4981 tactaggaat tctaggattc agtccgatgt tattgtcagt gatcaataaa gggaataaac
     5041 atggtgaatt gaaattgaat ccaaattatt acaacagtcc taacgaagaa ttgtttgtaa
     5101 aagaaacaga taaataataa aaaagactgc tctttcgagc agccagttaa aaaataatga
     5161 gtgtttattc ttgaaaaagc aacgcgctta tgaagcgcta ccaaaaattt atcatatttt
     5221 attaaaaata acaacccagt aaaaaatgac tggtaaaaat tcacaaagaa acggaaagaa
     5281 gcaaccgcag aaaatcaagc ttaaaaagag cttttgacct agaccaatcg aaaatcacaa
     5341 agtgagcgcc tgtatctatt gctacaggcg ctacttggca tagtgtacat tatgtcaaca
     5401 agaatttaat ataacatatt ttcctaatgc attctaacta taccacatta tcaaaaatat
     5461 aaattatctt tactaaataa aatattgaca agcctatacc ttttgtttta tgattttcac
     5521 aaagaaaacg ttttctatat atttttctgt atcaggagga aattctatga aaaaatttac
     5581 tttaggggta gctttgttaa ctattggggc agttgtttta ccgtcaacta ctatagttta
     5641 tgcttcagaa aattcccaag tggtatcatt aaacgaaaac aatgctactg aaaatgtagt
     5701 tattgagtac actgaagatg gtatggtcgt aactaaatat gaggatggtt atagtgcgga
     5761 agattttgca aattcttcag atatcatgac aagagctgca gttgcaagat ggggcaaaaa
     5821 ggaatataca aatattgctg taaccactgg agtagctgct aatgctatta atgctgcctt
     5881 ttgggcagga gggaaagcta gcacttctat gtttggtatc ccaggttggg caattggtgc
     5941 tttgttaaca ggtgcaagtt ggactaaaaa aggtgcttct ccagggaatg cagttgcaaa
     6001 agaatgggac aaaaatggta atggctggat tggattttat taccaaaggg gatatgatgc
     6061 agctggaaga gttgtagcta ctagatattc aacgttataa gttaaggagt gatgtttttg
     6121 atatctaaat cattaaaatc attaaaaaca tttactaaaa agaatccgca aatagctact
     6181 attcgtattt ttatatttgc tatagctgtt gctataattg ttaatatgct aagcggaggg
     6241 caattataca aatctggttt atttttagtg ttaatcttag ttgtcttaga cataataaag
     6301 tct
//
LOCUS       DARBDV010000122         6287 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000122 DARBDV010000000
VERSION     DARBDV010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.125"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..177
                     /locus_tag="SCM56_002632"
     CDS             <1..177
                     /locus_tag="SCM56_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111363.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
     gene            190..690
                     /locus_tag="SCM56_002633"
     CDS             190..690
                     /locus_tag="SCM56_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEG1111364.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="SCM56_002634"
     CDS             787..2634
                     /locus_tag="SCM56_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEG1111365.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2637..3533
                     /locus_tag="SCM56_002635"
     CDS             2637..3533
                     /locus_tag="SCM56_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1111366.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3583..3972
                     /locus_tag="SCM56_002636"
     CDS             3583..3972
                     /locus_tag="SCM56_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1111367.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3959..>6287
                     /locus_tag="SCM56_002637"
     CDS             3959..>6287
                     /locus_tag="SCM56_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG1111368.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2118 a          933 c         1348 g         1888 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atcatatcaa aatctctatt ctgagagaag tagttcctcg cgcgaaattt
     2821 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
     2881 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
     2941 ttattgataa gctgacaaaa aattccatta ctaatcaaga tatatataat gacatacttg
     3001 atataagagt tagtggggaa agtagtattt tttcagataa aaaattaatc aattctataa
     3061 gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct taagccaaat gatttagtag
     3121 ttatccgact tgaactaaat ggaaaaaaag ttagtttatc gaatgctagt gctggtcaaa
     3181 agacatctgc aattctttct atgattttgg cgtatggaaa ttcaccactc atattggatc
     3241 agcctgaaga tgatttggat agtcaattaa ttaataactt aatcgtaaga agtatcatta
     3301 caaaaaagga aaacagacaa attttaattg taactcataa tgcaaatatc ccagttaatg
     3361 gtgattcaga atggttgatt agcatgggag attcaaaaga aatatcggct gatcagtgcg
     3421 ggtcggtaga tgaacccaat atcaagccaa gaatatgtac tgtgatggaa ggtggcgagg
     3481 atgcttttac aaatagagcg aaacgctatg gttttaaaca aattgtagaa taacactaga
     3541 gggtgagaca tattattatg tccgcccttt ttaggaggaa aaatgaaaaa aataaaaagc
     3601 tataccagta tctgggcagt tgaaaaagtc atttacgcaa tcaatgattt tcagttgcct
     3661 tttcctgtga cttttaacca aatggcttgg ttcgttttat ctttactgtt tgtcatcgta
     3721 tttgcccatg tgcctccact atctatgata gaggaggtct ttttaaaata cttaggcata
     3781 ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg atggcaagaa accactggga
     3841 tttttacgat cctttatcag ctatcacttg cgatttaagg tgacttttgc aggaaagaaa
     3901 gtcaaagaac agaagaaacg atgggatgaa cccagtactc ttgtaaggag tgtgaactat
     3961 gtatccgatt aagtatattg aaaataatct cgtctttaac caagaaggcg agtgttttgc
     4021 ctactatgaa ttagtgcctt acaattactc ctttttgtca cccgaacaga aatttcaagt
     4081 gcatgataac tttcgccaac taattgcgca aaatcgtgaa ggcaagatcc atgcgttaca
     4141 aattgccaca gaaagtagta ttcgcgccac acaagaacga tcaaaaaaag aaattaccgg
     4201 tagactcaaa gaagtagcga aacaacgaat tgatatacag acagatgcat tagtatccat
     4261 gattggcgat agtcagattg attatcgctt ttttattggc tttaaactaa ttgcgacaga
     4321 tgaagaagtc aatctaaaga gtctcaagaa atctttcttt tctgggcttc aagaatttgt
     4381 ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg
     4441 ctgctattct aagcttgaaa aattaatgga aagcaaattg gctcgaagat ttaaagtcag
     4501 gcgagtcaca ccgagtgacc tgacgtattt gattgaacat atttatggcg aaaaagggat
     4561 accgtttgaa gaatacgaat ttcagctacc aaagaaaaag ctaaaatcag aaacactggt
     4621 gaaacgttat gacttattac gtccaaatcg ttgtttaatt gaagaaaaac caaggtgctt
     4681 acggatggaa catgagaatc atgagtcgta cgtagcgtat ttaacaatta atacaattgt
     4741 gggggagatg gaatttcctt cctcagaact tttctattac cagcaacaac aatttacatt
     4801 cccaatcgac acaagtatga atgtagagat tgtgacgaat aaaaaggctt tagctaccgt
     4861 tcgtaataag aaaaaagaac tgaaagattt agacaatcat gcttaccaat ctgacaatga
     4921 aacgaattcc aatgtcttag atgctttgga ttccgtggat gaattggaaa ccaccttgga
     4981 tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt cgagtgagtg ccgaatcagt
     5041 ggatgaacta aagcgacgct gtgatgaagt gctcgatttt tatgatgata ccaatgtaaa
     5101 actagtccgg ccctttggcg atatgatggg gctacatgaa gaattcttac cctcaagtaa
     5161 acgatatatg aacgactata ttcagtatgt tacgtctgat tttttggcgg ggcttggttt
     5221 tggggctact cagatgctag gagaattaga agggatttac tttggctaca atgtggatac
     5281 aggtcgtaat gtttacctga aaccggcatt ggcttcacaa ggagtcaagg gttcagtaac
     5341 caatgcgctg gccgccgctt tcctgggttc tcttggtgga ggtaaatcct ttagtaacaa
     5401 tctcttagtc tactatgctg ttttgttcgg tggtcaagct gtgattgtcg atccaaaagg
     5461 ggagcgagga gggtggaaag aaaccttgcc agaaattgcg gatgaaatca gtatcctcaa
     5521 cttgacgagt aagtcagaaa atcaaggtct gcttgatcct tatgtgatta tgaagaaaaa
     5581 gaaagactcg gaaagtttag caattgatat tctgactttt ttaacaggaa tttcaagtcg
     5641 agacggggag aagtttcctg tgttacgtaa agccattcga acggtgactc aaagtgatca
     5701 acgggggtta ttacaagtta ttaacgaatt gcgaaaagac gggtcaccaa tcgcggaaaa
     5761 tatcgccgat catatcgagt cctttaccga ttatgatttt gctcatttgc ttttctcaga
     5821 cggctcgatt acccaatcga ttagtttaga taaacagcta aatatcattc aagtggcgga
     5881 tttggtttta ccggatgcag aaacgacttt tgaagaatat accaccatgg aattgcttag
     5941 tgtttcgatg ctaatcgtca tttccacttt tgccttggac tttatccata gtaaccgaga
     6001 aatcttcaaa attgtcgatt tggatgaggc ttggagtttt cttcaagtgg ctcaaggaaa
     6061 aacgttatcg aataagcttg ttcgtgctgg tcgggccatg aatgctgggg tctatttcgt
     6121 cactcaaaat agcgacgatt tattagatga acgtttaaaa aataatatcg ggttgaagtt
     6181 tgcttttcgc agtaccgata ttcatgaaat caagaaaacc ttggaattct ttggtttgga
     6241 tcaggaggat caaagcaatc aaaaacgcct gcgagatttg gaaaacg
//
LOCUS       DARBDV010000123         6173 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000123 DARBDV010000000
VERSION     DARBDV010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.126"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            725..811
                     /locus_tag="SCM56_002638"
     CDS             725..811
                     /locus_tag="SCM56_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEG1111369.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="SCM56_002639"
     CDS             complement(932..1621)
                     /locus_tag="SCM56_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111370.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="SCM56_002640"
     CDS             complement(1628..1906)
                     /locus_tag="SCM56_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111371.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="SCM56_002641"
     CDS             complement(1866..2780)
                     /locus_tag="SCM56_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEG1111372.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="SCM56_002642"
     CDS             complement(2762..3142)
                     /locus_tag="SCM56_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEG1111373.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="SCM56_002643"
     CDS             complement(3592..3879)
                     /locus_tag="SCM56_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEG1111374.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="SCM56_002644"
     CDS             complement(3899..4123)
                     /locus_tag="SCM56_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HEG1111375.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="SCM56_002645"
     CDS             complement(4578..4748)
                     /locus_tag="SCM56_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111376.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="SCM56_002646"
     CDS             complement(4763..5296)
                     /locus_tag="SCM56_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEG1111377.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="SCM56_002647"
     CDS             complement(5317..6159)
                     /locus_tag="SCM56_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG1111378.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1152 c         1042 g         2346 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaacaaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DARBDV010000124         6068 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000124 DARBDV010000000
VERSION     DARBDV010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.129"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1080
                     /locus_tag="SCM56_002648"
     CDS             <1..1080
                     /locus_tag="SCM56_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HEG1111379.1"
                     /translation="EKYIFLYYISYVWNQFLIQFAKGLEKVKAMGIAGIISTIVTIIA
                     NIFFLLILKWGLIGFFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSI
                     PLIATVVGWWINSTADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAI
                     KEYGDNETAKFYGNTFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLI
                     ANVLNCASGLLGPILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVIC
                     SYVIYVVRKRAVGKDIFIKHYHIVVESWGLLFVQAIIEIYFKNYVIEIIIMIILIIIN
                     ISYIKQLCYRGRNIISMIKSREHKR"
     gene            1116..1832
                     /locus_tag="SCM56_002649"
     CDS             1116..1832
                     /locus_tag="SCM56_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEG1111380.1"
                     /translation="MVDNYERKNILKKKSIGCRMKIVAIVPMKLNNKRLPQKNTKAFT
                     NGKPLCYYILSTLLKVKQIDKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQV
                     LAGFTKEVEADVYVMTHTTAPFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGE
                     PFNYELDNIPRTQDLPPLYEETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDID
                     EAEDFMIADAIYNHLILQGS"
     gene            1851..3434
                     /locus_tag="SCM56_002650"
     CDS             1851..3434
                     /locus_tag="SCM56_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HEG1111381.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYM
                     ETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSH
                     IDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGL
                     MAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMRDELSEL
                     AKKVKVTFLTDPRWVRIDK"
     gene            3569..4147
                     /locus_tag="SCM56_002651"
     CDS             3569..4147
                     /locus_tag="SCM56_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111382.1"
                     /translation="MISILCIVAYHANLRLNYNADGIIVHGSLTANQIFLNYIGIWGQ
                     LGVNCFIFISAYFLSEKRNIHFENVLGIVLECIFYSVLVYGILCACGIETFSLNSFVK
                     EILSFALGRYWFITSYLALYILSPYLNIMLNNLEKLQLQKISMLLFIGVCCYRMLFKT
                     SLFGDCAVLIALYVICYTLKKTIIIFLRDTES"
     gene            4093..4611
                     /locus_tag="SCM56_002652"
     CDS             4093..4611
                     /locus_tag="SCM56_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111383.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            4619..5659
                     /locus_tag="SCM56_002653"
     CDS             4619..5659
                     /locus_tag="SCM56_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEG1111384.1"
                     /translation="MRRKEVKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVA
                     VSLAIFGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQR
                     PSKVIYDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAK
                     KRGITISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINS
                     GELNKDGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYN
                     IQIVDRVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYN
                     LMNSGGNGRVLR"
     gene            5908..>6068
                     /locus_tag="SCM56_002654"
     CDS             5908..>6068
                     /locus_tag="SCM56_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG1111385.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT         2199 a          694 c         1175 g         2000 t
ORIGIN      
        1 gaaaaatata tttttttgta ttatatatcc tatgtatgga atcaatttct aatacaattt
       61 gctaaagggt tagaaaaagt taaagctatg ggcatagcag gtattatcag cacaattgta
      121 acaataattg ccaatatttt cttcttgctt attctgaaat ggggattaat tggttttttc
      181 attgcaaata ttttatcaca gattatttca gctacttatc tgtcagtatt agtgagaata
      241 tgggaatata tcgattttga ggctaaagat cctcagctaa agagagaaat gctaaattac
      301 tcgattccat taattgcaac tgttgttgga tggtggatta atagtacagc tgataggtat
      361 gtggtttcct ttatagtagg tgtctcggcg aatggcttat tgtctgtggc atataaaata
      421 cctcaaataa ttaatacttt gcagggaata tttatacagg cctggcaaat ttctgcaatt
      481 aaagaatatg gagataatga gactgcaaaa ttttatggaa atacatttgt tatgattaat
      541 ttgttaatgt gcataacctg ttcaagtctt atcatattaa ctagaccgtt ttcacatatt
      601 ctttatgcga aagactttta tgaagcgtgg aaatatgttc catttttatt aatagctaat
      661 gttttaaatt gtgcatcagg tctgttagga cctatcttat cagcaaaaaa agattctaaa
      721 tccatgatgt ggtctgcaat cataggggca ttagttaatg tgattatgag tgtagtcctt
      781 gtttataaga taggggttca aggtgccaca atagccactg ttatatgttc atatgtaata
      841 tatgttgttc gtaaacgagc tgtgggaaaa gatattttta taaagcatta tcatattgtt
      901 gtggaatctt ggggattatt gtttgttcag gcaatcatag aaatatattt taaaaattat
      961 gtaatagaaa ttataattat gattatttta attattataa atatcagtta tattaagcag
     1021 ttgtgttata gaggaagaaa cattatttca atgattaaga gtcgagagca caaacggtag
     1081 acgatgttat ttttataaaa agatataatt tattaatggt cgataattat gaaaggaaaa
     1141 atattcttaa aaaaaagagt ataggatgtc gtatgaaaat agtcgctata gtaccaatga
     1201 agttaaataa caaaagattg ccacaaaaaa acacgaaagc ttttaccaat ggaaaaccat
     1261 tgtgttatta tatactttca acacttttaa aagtaaaaca gattgataaa gtgtatgtct
     1321 attgtagtaa cccagatatt aaagaattta taccagaagg tgtaacattt ttaaagagat
     1381 ctagttcctt agatcaggat acaacgagta ttacacaggt attagccggt ttcacaaaag
     1441 aagtagaggc tgatgtatat gtaatgacac acacaacagc accatttatt agcgaaaaat
     1501 ctattgaaaa aggactagat gccgtgctga caggagaata tgattctgca tttgctgcaa
     1561 aaaaattgca agatttctta tggaaagacg gtgaaccatt taattatgaa ttggacaata
     1621 ttcctagaac acaggatttg ccgcctttat acgaagaaac tagtggattt tatatttata
     1681 atcgcaatgt gatgaccgag ttgcatagaa gaattgggaa aaaaccttgc atagttgaag
     1741 taggtgagat tgagtcagtt gatattgatg aagccgagga ttttatgatt gctgatgcga
     1801 tttataatca tcttatttta caaggtagtt aagacagggg gaaggttagg atgaacaatg
     1861 tacagatttt agattgcaca cttagagatg ggggctattg taacaattgg gaatttggat
     1921 ttgataatac aaaaaaaatt atttctggac ttatagaatc aggaattgag attatagaat
     1981 gtgggtttct cacaaataaa acagtataca ataaaaatgt tacaaaatat acgaccttag
     2041 aagaattagc acagataatt cctgaaaaaa gagaaggaaa attatttgta gcaatgatga
     2101 actatggcga atataatata gaggatcttc cagattatga tggttcatct attgatggaa
     2161 ttaggattgc gtttcataaa aaaaattgca atgaagcgtt gaaattatgt cgtgagataa
     2221 agaaaaaagg ctacttagtt tttgttcaag ctatggtatc tctcaattat acagatgaag
     2281 agtttttagc agtaataaaa aatgtgaatc agtttaaacc atacgctttt tatattgttg
     2341 atagttttgg aatgatgaaa ggaaaagatt taactcgtct tttttacatg attgagcata
     2401 atctagaaaa agatatacag ataggttttc actcacataa taacatgcag cttgcatatt
     2461 ctaatgcaca aaaacttata accgtacaaa caaatcgaag cctgattatt gattcttcgg
     2521 tgtacggaat gggaagagga gcaggtaatc ttaacacaga actatttgta gaatatttga
     2581 atgataatgc aggaaaaaaa tatcaattaa aaccattatt atatataatt gatgaaatac
     2641 taaatagttt ctatcagaag agttattggg gatattcatt accaaactat atttcagcag
     2701 ttcataatgc tcatcctaat tatgcaagct atctgtcata taagaatact ttaacaattg
     2761 aagcaatgaa tgaaatattc gaaatgatgg atgaagaaaa aaaggtatct tatgataaac
     2821 aatatataga agatttatac tacaaatata tggaaactgg gaaaatacag gaggaatata
     2881 tggaagaact gagaaaattt ctctcagaca aaaaaattct actgattgca ccaggaaaaa
     2941 gttctgtaga tgaggtagaa agaataaaag agtttgttac caaagaagaa gtcgtaataa
     3001 ttagtgtaaa ttttgaatat tctcatatag atatagatta cgtattttta agtaatttaa
     3061 gacgttttca tgaattaccg aaagaaagca gaactaaatg tattgttaca tcaaatatac
     3121 aagcatcgga tgtatattta cggacgagtt ataaaaagtt gttatctgaa agggaagttg
     3181 taagagacaa tgcaggatta atggcaatta gatttttagc cgatatgaat gtggatgaaa
     3241 tattcttagc tggatttgat ggatattcac ataatgaaga tgagaattat ggtgagcaat
     3301 ccatggaaat tataacaaaa tttgcattat tagatgcaat gaacgtgcaa atgagagatg
     3361 aattgtcaga attagctaaa aaagtaaagg tgacattttt aacggatcca cgttgggttc
     3421 gaattgataa gtagtagtaa taatgttttt aaaattttga taaagagata gtgagagaat
     3481 aaaaatgggt aaagtcttaa ataaaaaaga agaaaaatat ttaagggacg atggggcgag
     3541 atgaaactct aattttgaac ttttaagaat catttctata ttgtgtattg ttgcttacca
     3601 tgctaatttg cgtttaaatt ataatgcaga cggaattata gtacatggtt cattaactgc
     3661 taaccagatt ttcttgaatt atattggtat ttggggtcag ctaggagtta attgttttat
     3721 atttatttcg gcatattttt taagtgagaa aagaaacatt cactttgaaa atgttttggg
     3781 aattgtatta gaatgtattt tttatagtgt attagtttat ggaattcttt gtgcatgtgg
     3841 aatagaaaca ttttcattga acagctttgt aaaagaaata ttatcatttg ctctaggtag
     3901 atattggttt attacatcct atttagcatt gtatattcta agtccgtatt taaatatcat
     3961 gcttaacaat ctggaaaaac ttcagttgca aaaaataagt atgttattgt ttataggtgt
     4021 atgctgttat cgtatgttat ttaaaacatc tttgtttggg gattgtgctg ttttaattgc
     4081 actatatgtc atatgttata cactgaaaaa aacgataata atatttttga gagacacaga
     4141 atcataggaa caattgtatg tataacagtt ataccacttc tcatatccat aggaatactt
     4201 ttcaaattta taccaaatgc aatggttaac cagatggttc atattggttc tccattgatg
     4261 gttatagcaa gtatattttt attttatgta tttaaaaatt tttcatttta cagtaagtat
     4321 ataaataaac ttgcgagaac cagtttaggg gtatatattt tacatgaaaa tcattatttc
     4381 attgatggta tgaagtctag aggcttaata tgggatggga tatttggatt aggcaaaagg
     4441 tttttagata gggatttttg gatttggttt ataattgcag ttgtagttgt gtgcataatt
     4501 tgcatggcta tcgatgttgt aagagaaaaa tttgaaagac agatttttaa aattaagagt
     4561 gtaaagaacc tgtgtaaact tatgaatcat gttataaata tatacccata attaataaat
     4621 gaggagaaaa gaagtgaaag ttatatcatt cggagaatta ttattgagac ttgcagcacc
     4681 cggttattca aagttatttc aaagagatag tttggagact acattctgcg gaggggaagc
     4741 taatgtagcg gtatccttag caatatttgg atatcaatca gaatttataa caaagcttcc
     4801 ggattcagat gttggatatg cggcagctaa tgctttgcgt tattttggag taaatgttga
     4861 taatattata tttggagagg gacgcatggg actgtattat ttagaaaaag gagcagctca
     4921 gagaccatct aaagtaattt atgacagaaa attttctgca atttcgttag ctgatagtga
     4981 tgaatttaag tggaaggaga tcctaaacga tgcaaattgg tttcattgga cagggataaa
     5041 tccagcattg tctgagaatc tctttgagac gtccttagat gcatgtcagg ctgccaaaaa
     5101 aagaggtata acaatttctt gtgatttaaa ttatcgatcc aagctttgga catcagagga
     5161 agcaaaagaa aagatggaac agatactacc attggttgat atttgtattt gtaatgaaga
     5221 agatgcagaa aaagtgctag gaattaaagc tgagcataca aatattaata gtggagaatt
     5281 aaataaagat ggctatgtgc aggtggctag ggagattcac aagaagtttg gatgtaggta
     5341 tgtagctaca actctgcgta gaagttattc ggcaagtaga aatggttgga gtgcaatgtt
     5401 atacgaagca gaaaaagata aagcgtattt ttccacagaa tacaacatac aaattgttga
     5461 tcgcgttggt ggtggagaca gcttttctgc tggtataata tatgcgttat gtgaaaatat
     5521 gtccggacaa aaggctattg aatttgcgac tgcatctagt tgtttaaaac atactataga
     5581 aggtgatttt aatcgaacta caattgagga tgtatataat ttaatgaata gcggtggaaa
     5641 tggtcgcgtg ttgcgttagt caacatataa agataaatca cagtaatttt tatggtgatt
     5701 atctgtctta gtagtgatta gaataacgtg aaaataaatt gttgagcgtt tatactgttt
     5761 tgaaaaataa atttaacata ttgaaaaata caaatatata cgtatattat aaagcgccga
     5821 ttgtaaaatt aagctagaca actaaaacag tcatttgtgc tacactcaaa atagttccga
     5881 ccaaagaatt ataaggagtg agtgcaaata acctatacct atcttacaac ggatgaactt
     5941 gtaataatag agtcttattt gaaaattaac caatctgttg ctaaaactgc gcattgcttg
     6001 aatcgttcaa gtcaaacgat ccataaagta tatctgttct tcaagcaaag aaaatcagcc
     6061 ttagaata
//
LOCUS       DARBDV010000125         6001 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000125 DARBDV010000000
VERSION     DARBDV010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.130"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..395
                     /locus_tag="SCM56_002655"
     CDS             <1..395
                     /locus_tag="SCM56_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111386.1"
                     /translation="ISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLSHETIKKLRIGG"
     gene            398..1228
                     /locus_tag="SCM56_002656"
     CDS             398..1228
                     /locus_tag="SCM56_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HEG1111387.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1244..2095
                     /locus_tag="SCM56_002657"
     CDS             1244..2095
                     /locus_tag="SCM56_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG1111388.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2092..2451
                     /locus_tag="SCM56_002658"
     CDS             2092..2451
                     /locus_tag="SCM56_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HEG1111389.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            2466..2627
                     /locus_tag="SCM56_002659"
                     /pseudo
     CDS             2466..2627
                     /locus_tag="SCM56_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2624..2929
                     /locus_tag="SCM56_002660"
     CDS             2624..2929
                     /locus_tag="SCM56_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111390.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            2929..3240
                     /locus_tag="SCM56_002661"
     CDS             2929..3240
                     /locus_tag="SCM56_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HEG1111391.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            3237..3410
                     /locus_tag="SCM56_002662"
     CDS             3237..3410
                     /locus_tag="SCM56_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111392.1"
                     /translation="MMMPKEIYSDALRGARNQLKMAKRAYEIRPTIENERRVKAIRRR
                     CSIYGELQKEDSK"
     gene            3407..3823
                     /locus_tag="SCM56_002663"
     CDS             3407..3823
                     /locus_tag="SCM56_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HEG1111393.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            3823..4491
                     /locus_tag="SCM56_002664"
     CDS             3823..4491
                     /locus_tag="SCM56_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEG1111394.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            4488..4865
                     /locus_tag="SCM56_002665"
     CDS             4488..4865
                     /locus_tag="SCM56_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111395.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            4862..5161
                     /locus_tag="SCM56_002666"
     CDS             4862..5161
                     /locus_tag="SCM56_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111396.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            5151..5309
                     /locus_tag="SCM56_002667"
     CDS             5151..5309
                     /locus_tag="SCM56_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111397.1"
                     /translation="MKFKIFEEDTRYKLEKKLNEFAKNNEIQHISLTASKSGYTTYYA
                     AVVSYVIQ"
     gene            5403..5819
                     /locus_tag="SCM56_002668"
     CDS             5403..5819
                     /locus_tag="SCM56_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HEG1111398.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         2230 a          899 c         1391 g         1481 t
ORIGIN      
        1 aaataagcct caaagatgaa tcgaatacga ctgtgagtga tgctaatgtc ttagcaaata
       61 tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat gagattttac
      121 cagcgaatca agaatattac ttctacaaat gcattacaaa aagagtttgt tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgaaaag
      241 aagttgatca ttcagagtac acatttgcag cactctgcag aatccatcac acggagaagc
      301 acaaaatagg tgtgatcaat tttaaaaata aatatcaaat caaaggaatc aagttaagtc
      361 atgaaacgat taaaaagtta aggataggag gataaatttg gctgagataa gttggatcaa
      421 acttaaaact actatgtttg acgatgaaaa aatacggtta atccaagctg ttcctgagtc
      481 ggatgccatc atcgttatat ggattcgatt actagtttta gcaggaaaga ctaacgacga
      541 tggtctgata tatatccaga ggaacatgcc ttataccgaa gaaatgcttg ctacattgtt
      601 tggcaaaaac gtaaatacgg ttcgcttagc gctaactaca ttggcaaatt tcaacatgat
      661 tgatctaagc agtgatggac taattgccat cagtaattgg gaaaaacatc aaaatatcga
      721 gggtatggat aaagtaaggc taaaaaatgc tgaaagaaac cgtaaataca gggaaagaaa
      781 gagacaggaa cgtctcaaat tggaaaatga cgttagcgtg acgtcacgtg acggtacaga
      841 taaagatata gaagaagata aagatataga taaagaagaa aagaaaggta agtattctga
      901 cgaacactta cgccttgcta aaaaaatgca aaggaattta actgaagatt ttccaaaaga
      961 aatgaacaaa gtagatatcg aaaaatgggc agacacaatc aggttgatgg aagaaagaga
     1021 taaagcatct atagaagcga ttgagtatgt gatcaattgg ctacctacaa atgaattttg
     1081 gtttggaaat attagaagtg ctaagaaatt gagagaaaaa tttgagaagc tcaaattcga
     1141 aatcaaagca gacaagaaga atcataaaaa gcaaagtcaa aaactacagt acagcaatcc
     1201 tagtgaatat gacgacttgc caatttaaaa ggagatgcat cacatggaaa gcctagcaaa
     1261 tgctatggag aagctaataa gaagagtatt agtgcaaagc ggaaaatgtc cagaatgtag
     1321 cgaacctttg tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac
     1381 atgtatgagt tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat
     1441 atataacgac agcttaaaag cacgagcatt gagttttttt caaaatggtt cggtattaac
     1501 agataaaact ttgtttaaat gcaaaatgga gaattatcac gtagtggacc aagaaacgaa
     1561 aattgcttta gaaagagcta aaagctatgt aaatgatgtc ctactgaacc atcctgcaca
     1621 tttcattcta tcagggaaat caggaagcgg aaaaagccac ttatcaatgg cgacagcttg
     1681 ggaaatactt gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagagtt
     1741 attagagcaa ataaagtttt cttataacaa tgctgaactg agaaaagaaa ttgaaggatc
     1801 gcttatagcc gatatcaaaa caactgattt ggtggttttt gacgatattg gagctgaatt
     1861 aggtagcggg gtatcaaata gtaggcaatt tacaaacaac acgttaaaca cgctcttaga
     1921 agccagacag aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga
     1981 agcctacggt gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa
     2041 attccaacaa acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa
     2101 taccgtaata aaaaaactgt tcatcgaggt atcaagtttg attctatcgc agaagcagag
     2161 tattacgatc tagccttgtg gcaagctgaa gcgaacggct ggaaagtaaa acttcaggaa
     2221 agatttgagc tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat
     2281 attcccgact tcacatttta taaaaacggc aaactagtca aagtcgtaga tgttaaaggg
     2341 atgcagacaa aagatttcaa aatcaaagcg aaactcttct gcagtcaata tcgagtacca
     2401 ctgatcttag cgaagaaata tcggaatacg ttcaaggaag aacggtttta acgaggtgat
     2461 ccatcatgac aacagaagaa gtgattcaaa tgcgtattcg aagccttcag cgtgagattg
     2521 acgatctgta acggacaaag gcagtgatgg tcaatgaaac ggcgaggaag gcaatcgatt
     2581 tgcacataga aaatttaaga aggaaaatcc atcgattgga ggaatgagcg tggataagaa
     2641 agcagcaatg aaacgaatca tcgaactgac acattctgag aattggcaag aagacaaaga
     2701 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg gctgaaaagc ctaaacggaa
     2761 aacgccgaga aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag gtactgctga
     2821 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
     2881 atggattgat tcaaaaggac gacagtttag gtatgtggag gagaaataat gaacgaacta
     2941 atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcgga tccaaaagca
     3001 caatttttga aagtagctga ggaattcgga gaaattgcat cggcaatggc aagaagtaat
     3061 gatgagctat ttaaagatag cgtaggagac gttatcgtca cgctgattat tctttccatg
     3121 caaaaagggg caaacataca agagtgttta gaaatggcgt ataacgaaat caaaggacgc
     3181 acaggaaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggagga cagcgaatga
     3241 tgatgccaaa agaaatctat tcagatgcat taagaggtgc tagaaatcaa ttgaaaatgg
     3301 caaaaagagc atacgaaatt cggcccacaa tcgaaaatga acgcagagta aaagcaattc
     3361 gccgtagatg ttcaatttac ggcgagttac agaaggagga cagcaaatga taccgagatt
     3421 tcgagcttgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc attttactaa
     3481 aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga aatcatatca
     3541 cgtgcgaaac atcgagctca tgcaatcaac agggctgaaa gataagaatg gcattgagat
     3601 tttcgagtgg gatattgtat cagtcagcgt gcgaaatgga ttcgattatt tagataataa
     3661 agtttgtgtt gtcaaaaatt caataggaca ttcgggatta gtgtgtgcca ctgttgatga
     3721 agatttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt atgaagtcat
     3781 cggaaatatt tgggagaata gcgagttatt ggaggaacag tgatgaataa acaggaattg
     3841 attgatgaat tagctaaata tgtaaagcgt tatgagaacg ttatggatga gcatggccaa
     3901 ggaaggtacg gcgcttatga agtatcttta aagttggtga aaagactaaa tgaatcaaaa
     3961 attacggacg aacaagcttg gaataaggta gctgaggctt atcctgaatc ggcacaaagc
     4021 ttgagaaaca ctttagataa tgctgtattt ggtaagactg gtgaaccgca gaagcacgtt
     4081 atgccgaagt ttgtggcgga gtggatagaa tacgctaaaa agaaaggtga tagtctagct
     4141 atttcattca agccgtggaa cctctatggt gttgagtata gcaaggctga tagatggatt
     4201 ggagataatc aagaaacgtt tgctcgtgct tggctagacg gctacgaggt cgagaaagag
     4261 ccgttgtatt atgtcccgct accatatgaa gtttgggatg aagaggcagc ggagcttaaa
     4321 acagagtatt tgtatctaca ttatgagatc acaagtgatg aaactagaat atttcctaca
     4381 aaagaaccta gaaaaggttt tgtcgcaaaa ttagatgaac ttacgattaa atcagcagat
     4441 gaaaactact ggccgtttgc tgtaccagtg gaagaggtgg tagaaggatg aaaaaattgc
     4501 accaactcat atctgagaaa gaaagcgaac tccaaaattt agaagatagt ttgggattgg
     4561 ggtttccaat tgttgagcaa gttaaaatgg ttcaaatcag tcatctacaa cttgaattgg
     4621 aagatttaag acaaatagaa gatccgtatc aactcaacga taaccagcaa atcgtgttgg
     4681 agtggttgaa actaactgcg tcaacaggaa agccaatgca agttgtattt tggatgatga
     4741 ataatgcagc atgggggcat ttagacgaat tgagggatcc tttgatggaa ttaactgaca
     4801 aacagcaatt cgaagtccta acagcattcg ctcaatgggg attagaacag gaggaaaaag
     4861 aatgaaacta aaagacggat tttacgctag tagccacggt atcggcggtt taatgctaga
     4921 catgccgaca aagaacccta aaacacgtaa gaagccaaaa gtcaaagtcg gtgacatggt
     4981 tcgctgtgaa gcagaaggat tcatctatcc atttcgtgga tatgtagaac acgtctataa
     5041 tcactcagcg atcattcgca ttgaaaacac gatggaatgc gacaagttat tagcgaaaag
     5101 caaagataat ttagcagtgg tgagattggt ggatatggag gttataaaca atgaaattta
     5161 aaatctttga agaggacact cgctataaat tagaaaaaaa attaaatgaa tttgcaaaga
     5221 ataatgaaat tcagcatata tctttaacgg cttctaagtc aggttataca acttactatg
     5281 cagctgttgt gagctacgta attcaataaa aggaactcgg caaataaaaa agccggatca
     5341 ctccgactga tgtaataaat ccgacaagtt tattatatca cataaaggag cggtttgact
     5401 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
     5461 tgaagaactt tcggcgcttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
     5521 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagaa gacgcgatca
     5581 ttcagatgat ggatatagaa gcggagagag acgcgatttt agcggctttg atggcgctta
     5641 gtctgattag ccgtcagata ctctactaca gcttctgtga cgtaaacaag cactctaatt
     5701 atgaaatagg gcaattgata cgaggatacg gagagaagaa tgtagagaag ctgaaatcca
     5761 tcgcattgat cgaatttgct gaagcataca aaaaaggcgt gttagttcag tatcgttaat
     5821 tttgtagggt ttttgtaggg atagtgtagg gtttttgagc ggtttaacgt gatattatga
     5881 tagtgtcgaa agattagtga taggtctaag acaaaataat aataaaagga acatcgtttt
     5941 attattgttt cacaattaag cttcgataga cagcagcgga aatattaaga ataaggatgt
     6001 g
//
LOCUS       DARBDV010000126         5985 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000126 DARBDV010000000
VERSION     DARBDV010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.131"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(451..1233)
                     /locus_tag="SCM56_002669"
     CDS             complement(451..1233)
                     /locus_tag="SCM56_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111399.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1298..2134)
                     /locus_tag="SCM56_002670"
     CDS             complement(1298..2134)
                     /locus_tag="SCM56_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG1111400.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2152..3561)
                     /locus_tag="SCM56_002671"
     CDS             complement(2152..3561)
                     /locus_tag="SCM56_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG1111401.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3575..5398)
                     /locus_tag="SCM56_002672"
     CDS             complement(3575..5398)
                     /locus_tag="SCM56_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1111402.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
BASE COUNT         1854 a         1285 c          969 g         1877 t
ORIGIN      
        1 cccttttata aaaagcaaaa aaaaaaagaa agaaacttat tcttgctaat aaactacgcg
       61 aaaggagtct aacgttgttt gatataactt agactgatcg ttaacttatt ttttatattt
      121 ctacatagtt atctttagaa ccatttcaga tacatatgaa acaattacat atgatttttt
      181 cataaaaaaa cagtactgtc tagtagtata aaatatacaa atttcttaaa aattttttca
      241 ctaaaatcac tacctgaaac agtctaaaac acttatatat caacgtttaa tgaaactatc
      301 tccgtttcat ttcagataca aataaatata gtcgggctcc aaatccgaaa aaaaataccc
      361 ccagtagctt atcctaagct actggagatg tataagtacg gtcatataac aacggggtag
      421 ggtaaataaa tgaaaaagat ggaagaatca ttattcaata acttttttaa atacttcgtt
      481 gtaatcagtt acctgtccgt tcttaaatac cacagaataa tcttttttcc cattttcaag
      541 taaccgaagt ggaatgtctt taccaacttg ctctgtcact gcattactga tacctgttag
      601 attagctaat aaatctgata aagattgagc atctgtagaa atctctgtat cagtatttat
      661 cgtgattcct ttatcttcat ttacaagctt ccaagtaggt ggtatagctg tgtatgtttc
      721 gatgaatgaa tcaattcttt catctatcgt cttcccagta tctacttctg tctcattact
      781 cttagatgaa tcgattcttt catccatcgt ctgctccgta actgcttctg tctcattatt
      841 ctcaccggaa actctcttca cacccaaact tatatattgt ccatctttag tgtgcatagt
      901 taaaattccc acgcttttac taataaattc actataacca gttctttcaa tagagacttc
      961 aatgtatttc acactatcat actcaaaaga aaaatagaca tgtaaaagca ttttgttgtt
     1021 tacatctaca taataatcct ttgactctac ttgagacctt cctatacgga atacagcttt
     1081 actgtctgat atctctatac cagcatcctt attattaatc tgccaatctc caagtagctc
     1141 ttctggcaag ctagtagttt cttgtgaaca ctttaccact tcactggtag aggtcgaaac
     1201 attcacctcc gcactgttag aagaattttc caaggaatca ggagccttag cacaaccaac
     1261 aagtctttat atattgtgca gccgatattt tcttcattca gtacaccctt gaaatatgca
     1321 gtgttagata aagcagttcg tcctgatttg ctgaggcacc catctcttct tgaacgtact
     1381 ccttgatttt caaggccgcc cgatactctt tcggaaaacg ctgcttcact gaagccagca
     1441 aaaaatcatc cttctccggc aactcttcct tcttaatcat tcgggcaata aaaaactgta
     1501 aatgcgtcac aaatcttaag taaagcatcg aatctttatc aacctttttt tcgggaaaca
     1561 gatatcctag catagccaag atatccttta gtgctcttac cgagagcatg gtttcttcca
     1621 tatttgcttg ttcactttgg gcatttacaa tgtgaaacgc aatgtttcca gcctcctctt
     1681 ctggcaactg aacattcatt tttttgttga tcaattggat agcatagcag ccgatctcaa
     1741 attcttttgg gtaatacttt tgaatttcaa tcagcaggcg attttgaaaa acaatgcctt
     1801 tttgactgcg ttcaatggca tgcttcaagt gatcaaccaa ggtaaaaaat agacttttat
     1861 tcagcttaaa gtcaaacttg gcgttggcat aatcgataat tcgcttgacg caaataatta
     1921 gatcatccgg agcagcctca aacagtttaa tgtaatcctt ttttagagca tcagaaagtg
     1981 aatctaattt aaatactttt tggattttgt cagccggaac caaatcgcct ttctttagtt
     2041 gaaatccaat ccctttcccc atcaaaatta cttctttgcc ttgcttatct aagcaaagaa
     2101 ttaaactgtt gtttaacgtt ttaattactc tcattagatt caccgttctc tctaagctaa
     2161 gccatttctt gcgattactt tttgatacca aaagaagctt tcttttttta ttcgttttaa
     2221 ttctttaaga ttctcatcgg ttcggtcaac atatataaaa ccataacgct tattgaagcc
     2281 ttgatgggta ctaacaacat caatcgctga ccaaggtaaa taaccaaaaa catctacacc
     2341 atctgttaaa gctgcttgaa tagccagaat atgtttggct agataatcaa ttcgatcctg
     2401 gtcattaatt cgtctatctt cctcaactat gtcgggtgcc ccatagccat tttcagtaat
     2461 taaaataggt aaatcatagc gatcgtacaa tttcctcaat gttaatcgta gtccagtagg
     2521 gtcaactacc cagccatatg gcgttttatc tacataagga ttttcagctg cccggtagac
     2581 accttcttct cccaacatga tctgttgatc ccccgctcta gcggaaacat ctgatccgtc
     2641 atttttgctg gcagcgatgg tggcagttga ataataatta atcgcaataa aatcgggttt
     2701 accggaggat aaaattgcct gatcctctgc ctcaatttta ggatataatc cacgatcatt
     2761 caaatagttt tcaaacaaat aattgtattt tccccgaact gctacatcta agaaagacca
     2821 gcctctcaat gtttcccaat tatgggctgc aattgcatct tctggactgg aggtagcttg
     2881 atacatagcc gttaaattga ttgctggacc gatttttcct ttaagattca actgatgata
     2941 caagtgaatt actctagctt gagctaataa aacatggtga ttaatttgaa agagttcttt
     3001 ttttgaaggc agctctccac ttggtggcaa accaatcgct ccaggatgaa ggatcatcgt
     3061 gttttgctca ttaattgtca gccaatagtt caccttgtca ccatactcag taagcaatac
     3121 ctgtacatag tccacaaaat aatcgatagt tgctcgattt aaccaaccgc cctgctcctc
     3181 tagaaaataa ggtaaatcga aatggtacat ggtgacaatc ggttcgattc cctgattcag
     3241 gcattcatca attacttgat gataaaaaga aattcccttt tgattggttt tattattttc
     3301 aatatccata attcgcgtcc aagcgatgga aaagcgaaaa gcttttaagc ctaattcttt
     3361 aaacagctgt atatcttctt tatagtgatg gtaaaaatcc gaggcaactt caaaattagc
     3421 taccccttct gtatgctgat acttgtcaat aatagacggt tgtttcccat cctcctgata
     3481 ggcaccctct gcctggtagg cactaatgga ggcaccccat aaaaacttct ctggaaatgc
     3541 tgaaagctgt ttgtattgca caaaaatcct ctccttaact aatttgcatc aatggttcgt
     3601 gttcctttaa gcttccgctt gcttcactaa ttcctgcgta attttcactg ttggtcacta
     3661 ccacaatcac tgtatcatcg tagccagcgt ttctaatggc agcgaaatcg gcgttgatta
     3721 ataattgtcc tgctgacacc tgatctcctg cttttacaaa tacttcaaag ggttcccctt
     3781 ttaaattcac ggtgtcgatc cccacatgga tcaataattc aataccttct ttagaacgta
     3841 gaccaatcgc atgtttagat tctaaaatag taatgacttc tccctcaaca ggtgaataaa
     3901 tgctgttttc ttctggatga atggctacgc cctttcccaa tagtccctta gaaaagacag
     3961 catcgttcac ttcagaaata ggacacacgg ttccttttac cggattttca acaatgactg
     4021 cttcggctat tgccgtttgc tctgattcat cagaagtatc gtcaaatcca attagataag
     4081 caatgataaa ggttgcaaca aaggcgatta cacacgtaat aattgcgtga acaatattca
     4141 ttggattttc tccaataaag gctggtaagg ctgctaagcc aggggaaaca aaggcatatc
     4201 tgactaaacc gctcaagcca gcatacaaac cagcaattgc accaccaatc atggaagcaa
     4261 ctaatggttt tttcagcttc aaggttactc catagagtga aggctcagta attcccatta
     4321 aagcagtgat cccagtggac acagctagct gcttcaactc tttgttttta gttttcgcgg
     4381 ctacagcaaa ggtagcacct gattgagcaa tattagaagc cagcattcct ggcccattga
     4441 tcatttcaga acccgtactg ctcaattgca tagtagcgat tggcgtcatt gcccaagctg
     4501 tacccgtaac caccaaaaat ggctgcaaac cacccatcaa aaatggaatt aaccaagctg
     4561 ctttgccgtt aatccattca gcccccattg ctataagatc gttcagcaac gttccaaacg
     4621 gtccaatgac aaccaacgct aaaggcccca taattaaaaa taccagcatc ggcttagtaa
     4681 agaatttaat aaacgaaggc gaaattcgat ccgcaaaggg ctctacatag gacatgatcc
     4741 aaacagtaaa gataatcggt aaaactgatc ccgcataatc tgcagctaat accggaattc
     4801 ctagaaaaga aacggcatcg ccagtagaaa aaagctgtga aatatttgga tgaagcaaaa
     4861 tgccagcaac agtaatcgcc aaaattgggt tgacatcaaa ctttatggct gccccatagg
     4921 ctaatagtac tggcataaaa taaaaggctg catcagcaat agtattcaaa atgatgtaag
     4981 tttgactact gtctgatagt acaccagtca aagtcaagac ggccaaaatg gcttttacca
     5041 tcccggcacc tgttatagct ggaatcatcg gtgtaaaggt ggtagaaatg acactaatca
     5101 atcttgaaat aatactttct tgattgtctg aagaggggcc ctttttaatt gaagttccca
     5161 cttccttttg aatttccttg aaggctgttt ggacttcatt gccaatcacc acttggtatt
     5221 gtccaccgct atccaccaca ctaattacac caagtgtttt ttctaaggct ttcgtatcgg
     5281 ctttcctttt atctttgagt tcaaaacgca gtctggtggc acaatgggtt aagctgacca
     5341 cattttcttt tcccccaacc tgttgcaata catttcttcc tacttctctg taatccatta
     5401 ttctctctcc ttaaagcagt aaaaaagacc aagtcaaacc acgccccaat caacattgaa
     5461 gtgttagttt gtcttggtct tgcctgcata atcagtaaca tgccaataac gttattttgc
     5521 tgtaatccaa taatagcaca ttatgatagc gcaatcaaca gggaatttaa ttttttatct
     5581 tttcgtttga ccgtataggg taatataaca gttagatcac atcaactagg gtatatctgc
     5641 gtatacttac tgctaaaata agtatatcta atattaacta taccagtaaa accattccta
     5701 cataaccagc gggaaattcc caaaatcgtg aaagcatcaa aaatgcatat aacggatcac
     5761 acaacttcaa caattctgct cttgggaaaa attgatgaat ttggtgagta aaggatggaa
     5821 taaacagcat tccaacaata taggcaacaa tagtgatact tgtttttttg gtaaaaatca
     5881 gtaagcaata gccaatttca atcattccga gatataaagg tatcgataga atgatttggg
     5941 ctagtattct taaaaattaa ctagcaaatg tttaaactga tttat
//
LOCUS       DARBDV010000127         5933 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000127 DARBDV010000000
VERSION     DARBDV010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.132"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..714
                     /locus_tag="SCM56_002673"
     CDS             <1..714
                     /locus_tag="SCM56_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HEG1111403.1"
                     /translation="STPFDEESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVL
                     LSTGMADIHEVSEAVDCFIDSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYS
                     DHSLGYDVGICAVAMGAKVIEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFL
                     GDGVKRAMPSELSTKEVSRKSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIG
                     KKVKRPISADSVLLEEDVE"
     gene            711..1859
                     /gene="neuC"
                     /locus_tag="SCM56_002674"
     CDS             711..1859
                     /gene="neuC"
                     /locus_tag="SCM56_002674"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HEG1111404.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            1832..2509
                     /locus_tag="SCM56_002675"
     CDS             1832..2509
                     /locus_tag="SCM56_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEG1111405.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            2499..3641
                     /locus_tag="SCM56_002676"
     CDS             2499..3641
                     /locus_tag="SCM56_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEG1111406.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            3654..4631
                     /locus_tag="SCM56_002677"
     CDS             3654..4631
                     /locus_tag="SCM56_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG1111407.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            4631..5539
                     /locus_tag="SCM56_002678"
     CDS             4631..5539
                     /locus_tag="SCM56_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HEG1111408.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            5540..>5933
                     /locus_tag="SCM56_002679"
     CDS             5540..>5933
                     /locus_tag="SCM56_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEG1111409.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHL"
BASE COUNT         2190 a          733 c         1134 g         1876 t
ORIGIN      
        1 tctactcctt ttgatgaaga aagtgcagac tttctaaaac aaattggtgt acaaggattt
       61 aaaattggct caggagattt aacaaattta ccttttctta gaaaaataga tatgtatggg
      121 ttgccagttt tattatctac aggtatggct gatattcatg aggtcagtga ggctgtagat
      181 tgtttcatag atagtccagt tactatttta cattgtactt caaattatcc ggctagttct
      241 gaagatatta atttattggc gattaacaca ttaaaagaaa tgttcgggat acccgttggg
      301 tactccgatc attcattggg atatgatgtt ggtatttgtg ctgttgcgat gggtgctaag
      361 gtaattgaga aacattttac cttggataag gaactacctg gtccagatca taaagcttct
      421 ttagatcctc aggaatttgc agattttgta aagcatatta gaaacactga aatattttta
      481 ggtgatggtg ttaagcgagc aatgccttct gaacttagta caaaagaagt atcaagaaaa
      541 agcctagtgg tcactgagaa tttaaatgtg ggagatattc ttacagagaa atctcttaca
      601 ataaaacgtc ctggaaatgg aatagctcct aagtttttat atcaatatat tggcaaaaaa
      661 gttaaaagac caataagtgc agatagtgtt ctgcttgagg aagatgttga atgaaaaaaa
      721 tacttatagt aagtggaaca cgagctgatt atggaatata taaacctgtt gctgagaatt
      781 tactacaaga taattgggat gtatcatttg ttgtttctgg aatgcattta tcccataaat
      841 atggatttac taaagaattg atcttagctg acggatttag aattagtgga gaagttcaaa
      901 gtttgttttt agagaacaat aagggaaaca tggctcggtc tgtggcatta gagataaatg
      961 gtttcacaaa tatatttgaa caaactagcc ctgattttgt attattacta ggggatagag
     1021 gagaaatgtt ggcagctgcg atagcttgtt tgtatcttgg aatcaaaaca gcacatattc
     1081 acggaggcga agtgtctggt actgtagatg aatcaataag acatgccata acgaaaatag
     1141 catctgttca ttttgctgct acggaaaaaa gcaaagagcg tttaataaaa atgggggaag
     1201 attcttggag agtttttccc gtcggagctc ctagaataga tactatttta aataatagat
     1261 tacctagttt tagagatata aaagaaaaat atcatctaaa atttgaaaag aaaaaatacg
     1321 ttttgcaagt atttcatcca gttgtaaccg aactagaaaa tatagaaaaa caaattttag
     1381 ctttgatcaa ttcaatggaa caacaatctc aaccaattat ttgcatttta cctaatagtg
     1441 atgctggtag cgaaattatc agaaaaatgt accagaaata tttaaaaacg gatattattt
     1501 atattacgaa tttagagccg gaggaatacc tgactatatt aaaagagtgc aaatttatga
     1561 tcggaaattc ttcttctggg ataattgaag ctgcatcatt caaaatacca gtaataaatt
     1621 taggaaccag acaaaatgga cgagaacaat ctaaaaatat tataaatgca aatctaaatg
     1681 aaaatgaaat tgaaactgct ttgaataaaa tacaagatcc tcagtttata aaaagcttga
     1741 aaaatgttaa gaacatctac ggtgacggca acagttctag aagaataaca aaaacattga
     1801 atcaaatatt gaatgatggg gaagatatta agtggattca aaaacaaata gcttattaaa
     1861 atatactagt gatccataca ttattattgg ggctggcgga cacgcaaaag tcattattga
     1921 aaccttaaga ttagaaaata agttaatata tggatttttt gatgataagc aaaataaaat
     1981 gcaagatctg aaatatcttg ggaaaataaa cgaattgaat agctgggcgg atagcaaaaa
     2041 aaaaatctat aaatttattg tagctattgg tgactgtgta gtaagaaaaa agattataaa
     2101 aaaattgaat atgccaagtg agcaatatgg aatagctgtt catccaagtg ctatagtggc
     2161 aaaagaaatt tcaattggtt atggaactgt tatttttgcg aatgctgtaa ttaacccaga
     2221 tgctgtaatt ggagagcatg ctataataaa cacaggtagc attgttgagc atgattgtag
     2281 aattaataac tatgttcatt tatctccagg tgtatgctta gcgggtggag tacatgtagg
     2341 tgtaggaact caaataggaa ttggttctca atgtatccag atgaagaaaa ttggatcttg
     2401 gtgtatgata ggcgcaggga gcactattgt taaggatatt ccctcacatt ccttagctta
     2461 tggcaatcca gctaaaatta agaaagaagg aattaatttt gaattctaga attttattat
     2521 caagtcctca tatgggagga acagaagaaa aatatgttgc taaggctttt aaagataatt
     2581 ggatagcgcc attaggtgaa aatgtggata aatttgaaga aagtattaca aaatatactg
     2641 gttctaaggg cggagcttct gcgttaagtt caggtacagc tgccatacat cttgccttaa
     2701 gactgcttaa tgttggaaaa aacgatattg tctttgctcc ttcatttact tttatagcta
     2761 cagtaaatcc tattttatat caaggggctg taccagtgtt cattgactca gaacctaata
     2821 catggaatat gagccctgtt gcactagaga gcgcattaga agaagctgaa agaaacaacc
     2881 aattacctaa ggcaatcatc gtagtgaatt tatatggtca aatggctgat tatgaacgaa
     2941 ttttagctat tgcaaaccaa tttaatgttc cagtgataga agatgcagcg gaatcgttag
     3001 gttctactta taagggaatt tcgtcaggct tatttgggac aattgggatc tattcattta
     3061 acggtaataa gatcatcaca acctcaggtg gaggagctct agtgtctagt gataaaaaat
     3121 atgttgagca ttcaagattt ttagcaacac aagcaagaga tcaagcaccg tattatcaac
     3181 atagcgaaat aggttataat taccgtctaa gcaatatttc tgccggtatc ggacgtggac
     3241 aaatggaagt attgaatgat agagtagcta aacgaagaga aattttcaat cgatataagg
     3301 aagctttttc tggaattgat agaattgaaa tgatgccaga agtggatggc atggtgccaa
     3361 atcgttggtt gtctacttta acgatcaatg attataattc tattgatcaa gtagttaacg
     3421 taataaataa actaaatgaa aaaaacattg aaacaagagc tttgtggaaa ccactacatt
     3481 tgcaaccagt atttcaaggg gtaaaaatgt attaccatga taataatctt agtgtgtcag
     3541 aaaagttatt taatagcggc ttatgcttac cttctggaag taatatgaca gatagtgaac
     3601 aagaatttgt tattgatgag tttaaaaaaa tattcaagta ggtgtgatat tttatgaaca
     3661 taggaattat tggtttaggt tccatgggga aaagaagact tagactcatt caagaacact
     3721 ttaatgatat tttactatgt ggtgttgata ctaatgaggc gagacgaaag gaagttgaaa
     3781 aggaatttgg aataccaact tataatagtc taacagaggc aaaaaatttg tttgaaataa
     3841 attcagtttt tgtttgtaca tcccctataa cccatgaatt tattataaaa gaggctttaa
     3901 atttagattg taatatcttt acggaaatca atttattaaa tgaatattat gatgaaataa
     3961 tcgaagtagc taaaaagaaa gatagattac tttatctatc ttctacattt ttgaagagaa
     4021 aagaaattca atatattaag tctaaagtga cgcataataa aaaaatttct tatcaatacc
     4081 atgtaggaca gtatttaccc gactggcatc cttgggaaga ttatcgcaat ttttttgttt
     4141 caaacaaaaa aactaatggg tgtagagaaa tattcgctat tgaattacct tggattatag
     4201 atacatttgg gaaagttaaa tcgtttactg taatgaccca gaaattatcc aatcttgata
     4261 ttgattattc ggacagtttc actcttatta ttgagcatga aactggtata gttggaactt
     4321 taagtgtaga tgttgtttca agagtagcta aaagaaattt aactataatt ggtgaaaaga
     4381 ttttaataga atgggaaggt aacccggatt ctctattttc atggaatgaa gaaacttcta
     4441 gtatgacaga tattcaattg tacgatgagg ttatgcatga taataattat tcacatacta
     4501 ttattgaaga tgcatattta gaggagatta aagaattttt cacgcttttg gaaaagaaaa
     4561 tggaacccca atatactttt gaaaaagata agtatattat taacttaatt aacgacattg
     4621 aggtaaatta atgatgaaag taattttttt tgggttaggg tcaattggaa agagacatct
     4681 aaaaaatcta ataagctatg gaaaaaagaa ttctattaat tttgatataa cagcttatag
     4741 tagcagtgga aaaaaagagt ttgaagaaat aacgtatatt caaaatagag acgatttgga
     4801 tacacattat gatatagctt ttattacaaa tccgactttt aagcatctgg aaacactaaa
     4861 tgtaattgtt gaaaaagttg attacatatt tttagaaaaa cctgcttttt ctgagagtat
     4921 tgatcttact caatataatt atgattacaa aaatatttat gttgctgctc cactgaggta
     4981 taaaaatatc atggaagttg ttcgtaatag tattcaaagt aaaaaagtat attctgtaag
     5041 agctatttgt tctacttatc ttcctgattg gagaaaaggt actgattata gaaataatta
     5101 ttctgccttt aaatctatgg ggggaggagt agaattagac tgtatacatg aattggatta
     5161 tattataaac ctatttggtt tccctaaatc agtaaaaaaa cagtttggta aatattcgaa
     5221 tcttgaaatt aacagtaatg atttagcaac atatatattg agatacgatg ataaaattat
     5281 agaaattcat ttagattatt ttggaagagt tcctacaaga aaatttcaat tgtttacttt
     5341 agatgaatgt atcaatgttg acttattaaa taataaaata agttttgaag aaaacattgt
     5401 taaagaaatg cctgaatctg ctaatgatat gtatgagaag gagttgaaat atttctttga
     5461 aaacgtagta acaggcaaag aaaactggaa tgatttatat catgcaaacg aagtattgaa
     5521 aatagctgag gagaaataaa tggaaatttt atttactatt tgtggtagag ctgggtctaa
     5581 ggggtttaaa aataaaaatt tgaaatattt ggcaggaaag cccctagtcc atttcgcttt
     5641 agcaatgata gatatctatc aacaaaaaca tcctgaacat aattgcacga cttgtttgaa
     5701 tacggacagt aatgagttag tagaattgac gactaagtta aatccgtctg tgaaaattgt
     5761 agaacgtaaa cctgaattag ctacagatac tgtagggaaa attgaagtga tacgtgattc
     5821 attcttacaa ctagaaagag aagataatat atttgattta attatagatt tagatttgac
     5881 ttcaccttta cgtgaagttt ctaatcttga agaattaata gatgaacatc taa
//
LOCUS       DARBDV010000128         5816 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000128 DARBDV010000000
VERSION     DARBDV010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.133"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1152
                     /locus_tag="SCM56_002680"
     CDS             <1..1152
                     /locus_tag="SCM56_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEG1111410.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="SCM56_002681"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="SCM56_002681"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEG1111411.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="SCM56_002682"
     CDS             2728..3003
                     /locus_tag="SCM56_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEG1111412.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="SCM56_002683"
     CDS             3009..3776
                     /locus_tag="SCM56_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEG1111413.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="SCM56_002684"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="SCM56_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEG1111414.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
BASE COUNT         1987 a         1033 c         1304 g         1492 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaat
//
LOCUS       DARBDV010000129         5809 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000129 DARBDV010000000
VERSION     DARBDV010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.134"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            450..1604
                     /locus_tag="SCM56_002685"
     CDS             450..1604
                     /locus_tag="SCM56_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HEG1111415.1"
                     /translation="MIILKPKNKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1598..2020
                     /locus_tag="SCM56_002686"
     CDS             1598..2020
                     /locus_tag="SCM56_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111416.1"
                     /translation="MVNKPIVLVMGLVLVLFLTSCSKKQEIQKTELIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            3143..4075
                     /locus_tag="SCM56_002687"
     CDS             3143..4075
                     /locus_tag="SCM56_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG1111417.1"
                     /translation="MANEIVKYKPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFDQLKDLSAYKQTAGEVFIKDLTRTYDHLMDLRFGKKSNGGLTIERFTMFTDFEIH
                     GEADNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRTKLFNIEHNGELTQEEKWRAKDRILDLPLGTHEAN
                     FKKQQQTEKEEVEKQTLSNELKQDLLGNLQNLFD"
     gene            4092..4607
                     /locus_tag="SCM56_002688"
     CDS             4092..4607
                     /locus_tag="SCM56_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEG1111418.1"
                     /translation="MADKTIKDLAEELGVSKTAINKKVSDENRKLWFAKIGNKFVINE
                     IGQKAIKSMFFTENENSNQQPVGEKSKTENHKNANQVSELELFFFLEKQLNQKDIQLQ
                     EKDEQLKQMQKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQ
                     NKEKKWWQFWQ"
     gene            4903..5184
                     /locus_tag="SCM56_002689"
     CDS             4903..5184
                     /locus_tag="SCM56_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111419.1"
                     /translation="MDILVRNINPKIITRVDKRCQELTTKTGEKWSRNKYLKVLIESD
                     FDRQLMNYKKEQFDLLLEKFIDIQTKNTETLNDYVHTNNQLIETLIGGI"
     gene            5186..5602
                     /locus_tag="SCM56_002690"
     CDS             5186..5602
                     /locus_tag="SCM56_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111420.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EKQLNPSQRNQPKNRQKETSQTFTSKNFDPTNFLKR"
BASE COUNT         2228 a          961 c          930 g         1690 t
ORIGIN      
        1 aaaaaaaaag actgctcgaa agagcagtct caagcaaaaa ataaacgaac aaacgcaatt
       61 actcacgaac acaaacaaat acaataaaat acctaaataa tgaaaataag tgataaaaaa
      121 caagggtatt tatagtttaa caagcgctca taaaaataac aagtctttaa aaaatgacag
      181 ataaaaatgc acaaagaatc ggacacagat aaccgcgaaa aatcaagctt tagaaagttg
      241 tttttagcta gaccaatgaa aaatcacaaa gtccgcccct gtatccgttg gtagaggggc
      301 tacttggttt agtatacatt atgttaacta gctcgttaaa taacgattat gttcccaaaa
      361 caatatttta tgaactataa caaaaatttg ttatcgcata ttgtatattt taattatagg
      421 tgtatatcac ttaattttaa cacaaaggcg tgattatttt gaaacccaaa aataaaattt
      481 atctaggtct cctcttttcc ttgatgattg gaatttttag tataggaatt acaaatacct
      541 atgcttcaga aaattctaat ttcaatatca actcccaacc aattgaagaa caagcaatta
      601 attttgcgaa aactgatttt gaacactcca taacttcaat tcttttaatg gaaaataaca
      661 ctgaaactga tattaactat actcttggtt cgccatttaa aatgctcgat ggagaaaata
      721 cggcaaatag ttctattttc tttccagtat tttcagacgc tgataatacg attaaatatg
      781 tttataccgt caaaaaaaat acaaatggag aatattcagg aagtatttca caatttttag
      841 caaaagaact tcaaaatcta gtagatagca atataacaga tacaacctta ttttttcaag
      901 ataatagcat ttattattcc gataatggcc aaattaaact actttggaaa tcacctttaa
      961 gtacagaaaa aaatggtgtc tcttccttta cagaaaaaaa tgatcttata tccgtaaata
     1021 taaaaaataa aattaaaaat ttctctaaaa aacaaaattt tcctgcggtt aaacgtgtaa
     1081 aaagtgtctc ctccggagat aactttatta tgattaattg gcgtatagca gaaacccaaa
     1141 caagttttcc gtggtgcgct gcttacgtag aagctgctat cttatgtaat aaaacagatg
     1201 tacgcgctac ttcagctaga gccattatgc attataccta tcctaattta aacaacagtc
     1261 aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat tactgtggtg
     1321 tttatcctaa aaaagtagca cgaatactaa acctttcaga ggtccaaaca caactaagaa
     1381 aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac agccgtcatg
     1441 cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat gtgtggaatc
     1501 cttggtataa ctacccaact gtaataagtg caaatgcagg gccagttaca attcctgtac
     1561 ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac ccattgtttt
     1621 agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac aagaaataca
     1681 aaagactgag ctgattcctt ttctaggaac ttggcaaact aaagatcact cagaacgtct
     1741 agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct tagtaaaaga
     1801 gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac aagttttcat
     1861 ttacaaagca acaaataatt tactaacact gcgctctaat aaaaaacaat tagattacac
     1921 ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg atgggacaga
     1981 agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg tttgatatta
     2041 caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt ggctagcatt
     2101 tgctagcctt ttttgtattg cggagcgtga gttttgaggg ggattcaagg gggagtttct
     2161 cccctttgac aagcggttat ttgcaccaaa tacgaaggta tttggctagc aaatatagag
     2221 cttgccaaac cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca
     2281 aacgatacgt cctatggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat
     2341 ttggctagca atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt
     2401 cgagtgagcg aatccgaatt tgcaaatctg aagctttgca aatgagtgtg ccggcttccg
     2461 taaaagtcta ggtaacatta gtcgatatat acataaggct ctaatttgcg ttttaaggcg
     2521 ttttaaacgt tctggtatgt aatttatacc aaagcgactt taaaacgcct taaaacgaat
     2581 tttataggtc ttgtaggacg tttcagggtt gttagggctt gccctgaccg cctgtaaggc
     2641 gctttaaaaa caagataagg gattttatct agacttgtct taaaacgccg tagatgagcg
     2701 ctcagagctt cttaaaggac tccttacgaa ctgtttttaa gcaaaggagc ctttaggcga
     2761 ctgcgcagtt caatgtgaga gcgtagcgat cacaccttta aagcgaccgt agggagcaga
     2821 ttcccagttt aggaaatttc gcgaacctgt ttggagaaaa aaagtaggaa atttttaggc
     2881 gggaaatttt gcccccctct tttataatct cttttataac ctctttttaa acctctttac
     2941 cgtcggctgt atccttactc tcccaaggcc tgtagctttt ttagggcatg aaattatctg
     3001 tttataggca tgaaattatc tgtttatagg catgaaatta tctgtttata ggcatgaaat
     3061 tatctgtttt aggtattgtt ttaagacata atatacctta aaattatgtc aataaaatta
     3121 gttagactgg agtatcaaat aaatggcaaa tgaaatcgta aaatataaac cagaattaaa
     3181 taccattcct ttaaggaaat ttactccaat agaaatgaac ttgttttttt ctattgtttc
     3241 tagaatgcgt gataaaggta atcaaaaaat acgcttttat ttcgatcagc ttaaagattt
     3301 aagtgcttat aagcaaactg ctggagaagt ctttataaaa gatttaacaa gaacatacga
     3361 tcatttaatg gatttacgtt ttggaaaaaa aagcaatggt ggattaacca tagaacgatt
     3421 tacaatgttt actgattttg aaattcatgg agaagccgat aacccttatg tggatataaa
     3481 agttcacgaa aaagctttgc ctttacttaa caatttagaa agttgggtac gtttctcttt
     3541 aacagaattt cgagatttaa atagcagtta tgctaaaaca atgtttagat tactaaaaca
     3601 ataccgaaca caaggatggg ctgaattttc caaggaagat tttttagaac tattagatat
     3661 tcctaaaagc tatagacaaa caaatataaa tcagtttgta ctcaaaccca taaaagaaga
     3721 actcacacca ttatttaaag gattaacgat tcgtaaaaaa tatggtaaag gccgaggcaa
     3781 acctgttatt ggttatcgtt ttacttggaa agctgaaatt aatcatgctg acgatttttc
     3841 aaaagggaag caagaagatt tacgtacaaa attatttaat attgaacaca atggagaatt
     3901 aacgcaagaa gaaaaatggc gcgctaaaga tcgaatacta gatcttccgt taggtactca
     3961 tgaagctaat tttaaaaaac aacaacaaac tgaaaaagaa gaagtggaaa aacaaacact
     4021 ttcaaacgaa cttaaacaag atttattagg aaatttacaa aacttattcg attaaagtat
     4081 aaggagtatt tatggcagat aaaacgatca aagaccttgc tgaagaatta ggagttagta
     4141 agacagctat taacaaaaaa gtttctgacg aaaatcgaaa actttggttt gcgaaaatcg
     4201 gaaacaagtt tgtgattaac gaaatcggtc aaaaagctat aaaatcaatg ttttttaccg
     4261 aaaacgaaaa ctcaaatcaa caaccagttg gcgaaaaatc gaaaaccgaa aaccataaaa
     4321 acgccaacca agtttccgaa ttagaactct tttttttcct agaaaaacaa ttaaatcaaa
     4381 aagatataca gctacaagaa aaagacgaac aattaaaaca aatgcaaaaa ttactcgacc
     4441 agcaacaaca actcacctta caagccaatc aacaaatcaa aaaacttcac gaacaactcc
     4501 agctaaccta tgcagaagaa tcttcagaaa aagaacccct ttctgttact gaagaaactc
     4561 aaaaaggaca aaacaaggag aaaaaatggt ggcaattctg gcaataaccc aacaattttt
     4621 attcataaaa attgttgggt tactttcttt ttttgctttt acaagcatag cgacatgatc
     4681 tcacgtcgct attttttaaa gagcataagg aaacgcggta tcactttctt acgaaaggtg
     4741 ctaggtaggc tgtgcctacc acgtttctaa gaaaaaaatt tgctatcact ttttcgccat
     4801 tgtggctagc ttatgtatag tatttcggta aaaaacagct atacaaatac tatatttgta
     4861 tgatatacat aaattataca cacataagaa aggacgtttt ctatggacat tttagttcga
     4921 aatatcaatc caaaaattat tacaagagta gataaacgtt gtcaggaact tacaaccaaa
     4981 acaggagaaa aatggagtcg aaataaatat ttaaaagtgc taatcgaaag tgattttgac
     5041 cgtcaactta tgaattacaa aaaagagcaa ttcgatttat tactcgaaaa atttatcgat
     5101 attcaaacaa aaaatactga aacattgaac gactatgttc atacaaacaa ccaactcatt
     5161 gaaacactta tcggaggaat ataacatgag aaaaacttct gttcaaaaac acgttaccct
     5221 ttatcccagt cacattgacg ttttagattc catcatcgaa aaaaatccag atatcaataa
     5281 ctactcggaa gccattcgct ttttatgtat gagttatgaa cctgatacca atacaacaac
     5341 taccagtaac caagccaaac taaatgcgat gggaaaagaa atttctatca tgaccgaaat
     5401 tatcgcagaa tttgctgatt tgtatttaaa agatacaggc gttcttatcg gtacaaactc
     5461 gaaagtatat caagaagtaa aagaaaaaat tgaaaaacag ctaaatccct cccaacgaaa
     5521 tcaaccaaag aatcgtcaaa aagaaacgag tcaaacattc acttctaaaa atttcgaccc
     5581 aaccaacttt ctaaaaagat aaaaaaataa accaggaaat ttttaatttc ctggtttatt
     5641 ttctagagag cggtcttcca ataagatttg caaatcgatc ccaatttgcg taaaaaaaaa
     5701 gctgctcgta cgagcagcta aacaagaaaa agtcagcttg ttttaagggt aaaataagtg
     5761 taacaaattt tgcgaatgca acgatctttt ttattatacg caaacaatc
//
LOCUS       DARBDV010000130         5791 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000130 DARBDV010000000
VERSION     DARBDV010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.135"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(99..1229)
                     /locus_tag="SCM56_002691"
     CDS             complement(99..1229)
                     /locus_tag="SCM56_002691"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEG1111421.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1288..1515)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1877..3079)
                     /locus_tag="SCM56_002692"
     CDS             complement(1877..3079)
                     /locus_tag="SCM56_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1111422.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="SCM56_002693"
     CDS             complement(3139..3342)
                     /locus_tag="SCM56_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HEG1111423.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="SCM56_002694"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="SCM56_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4879..5691
                     /locus_tag="SCM56_002695"
     CDS             4879..5691
                     /locus_tag="SCM56_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1111424.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1717 a         1189 c          922 g         1963 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta g
//
LOCUS       DARBDV010000131         5780 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000131 DARBDV010000000
VERSION     DARBDV010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.136"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            512..1069
                     /locus_tag="SCM56_002696"
                     /pseudo
     CDS             512..1069
                     /locus_tag="SCM56_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            1127..3166
                     /locus_tag="SCM56_002697"
     CDS             1127..3166
                     /locus_tag="SCM56_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEG1111425.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3275..4426)
                     /locus_tag="SCM56_002698"
     CDS             complement(3275..4426)
                     /locus_tag="SCM56_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEG1111426.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4580..5110)
                     /locus_tag="SCM56_002699"
     CDS             complement(4580..5110)
                     /locus_tag="SCM56_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEG1111427.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5209..5559)
                     /locus_tag="SCM56_002700"
     CDS             complement(5209..5559)
                     /locus_tag="SCM56_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEG1111428.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
BASE COUNT         2094 a         1057 c         1073 g         1556 t
ORIGIN      
        1 tataaaaaaa cacataaaac taaaaaatta tcaaagaatc ctggtgcaac tgaaagaatt
       61 tgcaacggga ttctttcaac ttatttgtta tttttttcta aaaaatatta aagattgtta
      121 aatttcacta tttcatttca aattccattg cctaatcatt aggaaaagtc tatcatgaac
      181 tcgtaaatag aatatagtaa taaccttgtt aggtgaggct cctatacaaa cataggctgt
      241 tgcccagaaa cgtcgaaagt cgccaatggg taaaacagaa actgttggat taaggctttt
      301 tccaatagct aagaatgttt tctttacgtt gtatagtgct aaaactcgga cgatgagatg
      361 atgctaggtc agaattcttg ttcgaaaagg tttctgacct tttctttata tttttgtgac
      421 tgacttattt gatgagacaa actcgctcct cacctgacaa gtggattgct gtgtgaggag
      481 gagatgcggt gaaaaaagat cgattggtgg aggtcgctcg gattcacttc tggttgcaca
      541 atacaaccag aaaacacaac aagcaaaact gatctctatc atgagagatt catatgtcga
      601 tattccaggt tacggaatga ataagataaa tgcagcttac tcttatgggg gagttgattt
      661 attaaatcaa acattaaagg aaaatttcaa atttgaagct ccctattatg caagtatcac
      721 atttcaagat tttatcgatt gcgttaatga actgtttcct ggtggagtca agatcgatgc
      781 agaaaaatct ctagatttag atggcgtata tataaacaaa ggagagcaag tgatggatgg
      841 taatacttta ctccagtatg ctcgatttcg ggaagacaaa gaaggagact tcgggaggat
      901 cagaaggcag cagcaagtga tcaaagctat ctctcagcag ctgaaagacg taacttcaat
      961 attcaagcta cccaaagcgg ttgggaaatt acttggtagt atccaaacga accttccaga
     1021 aagtgtgctt ctggattgtg gtatggattt cctcaaagac atatgttaga ataaacaggt
     1081 ataaatagtg aaataaagga atgacaagca agagaaggag gaaaaaatga aaagaagtga
     1141 caagcacggc aaaaatcgaa caggcgctta tattgccggc gcagtgatct taatagcagc
     1201 tgcgggtggc ggttattttt actaccagca ctatcaagaa acccaagcag tagaagctgg
     1261 agaaaagaca gttgagcaat ttgtccaagc tttgaacaag ggagattata acaaagctgc
     1321 agaaatgaca tcaaaaaagg cggcaaataa aagtgcatta tctgaaaaag agatcttaga
     1381 taaataccag aatatatacg gtgctgctga tgtcaaagga cttcagatat cgaatctaaa
     1441 agtagataaa aaagacgatt ctacttacag tttttcatat aaagcaaaga tgaatacctc
     1501 attaggtgaa ttgaaagatc tttcttataa aggaacatta gacagaaatg atggtcaaac
     1561 cacgatcaac tggcaaccca acttggtttt tccagaaatg gaaggaaatg acaaagtaag
     1621 cctgaccaca caagaagcag caagagggaa tattatagat cgaaatggtg aaccattagc
     1681 aacaacaggc aaactaaaac aattaggagt cgttccaagt aaacttggag atggaggcga
     1741 aaaaacagcc aatatcaaag cgattgcttc ctccttcgac ttaacagaag atgctatcaa
     1801 tcaggcgatt tcacaaagct gggtacaacc cgattacttt gtcccattga aaatcattga
     1861 tggagcaacg ccagaacttc cagctggagc taccattcaa gaagtagacg gcagatatta
     1921 tcctttgggt gaagcagctg ctcagctgat tggttacgtg ggagatatca cagcagaaga
     1981 tattgataag aatccagaat taagcagtaa tggaaaaatc ggtcgctcag gtttggagat
     2041 ggcttttgat aaggatcttc gtgggacaac aggtgggaaa ttaagcatta cagatgcaga
     2101 cggtgtagag aaaaaagttc tgatcgaaca tgaagttcaa aatggcaaag atatcaaatt
     2161 gacaatcgat gcgaaggcac aaaaaatagc ttttgacagt ctaggaggaa aagctgcatc
     2221 aactgttgcg acaacgccaa aaaccggtga tcttcttgcg cttgctagct ctccaagcta
     2281 tgatccaaac aaaatgacaa acgggatctc acaagaagat tacaaagctt atgaagaaaa
     2341 tcctgaacat ccattcatca gccgatttgc gacaggttat gctcctggat ctacgtttaa
     2401 aatgattaca gcagcaatcg gtctcgacaa cggcactatc gatccaaatg aagtgttgac
     2461 gatcaacggg cttaaatggc aaaaagacag ttcttgggga tcttttaaaa caacgcgtgt
     2521 cagtagtgat gtatcacaag tagacttaaa aactgctttg atctattccg ataatatata
     2581 tgcggcacaa gaaacgttga aaatgggtga gaaaaaattt cgtacaggct tagataaatt
     2641 catttttggt gaagaccttg atttgccaat tagtatgaat ccagcacaaa tttctaatga
     2701 agatagcttt aactcagata tcttgctagc tgatactgga tatggacagg gcgaacttct
     2761 aattactcct atccagcaag cagcaatgta ttctgttttt accaacaatg gcacacttgt
     2821 ctatcctaaa ttgattgcag ataaagagac aaaagataag aagaatgtca tcagcgaaac
     2881 agcagtacaa acgatcgtgc cagatctgag agaagttgtg caagatgtaa atggtacagc
     2941 acattctctt tctgctttag ggattccgtt ggcagcgaaa actggtacag cggaaatcaa
     3001 agaaaaacag gatgtaaagg ggcaagagaa cagtttcttg tttgctttca atcttgataa
     3061 ccaaggttat atgatggtca gcatgttgga aaataaagaa gatgatgatt cagcaaccaa
     3121 acgagcatcg gaactattgc aatacctcaa ccaaaattat caataaaaaa ttgcttttta
     3181 agtacagtaa aaggactgtg gcgttatcgt cacagtcctt ttttcaaata aacggttttc
     3241 acgatgtctc tttcacaact tctttttgat ttactcacac ttcataaact ttttttgtaa
     3301 aatatttgag aacaagtgga gaaataagtg tacttaataa aatcacgata actaaaggtg
     3361 aatagtactg tggttcgatc aattgacttt gctgtccaat ttgtaaaatg atcaaagcca
     3421 tttctccacg cgaaatcata ccagcaccga ccatcaatga actatgttga gaaaaaccgg
     3481 caatctgagc accaaaataa cctccaagca atttggtcaa aatcgctaca agcgtcaata
     3541 tagaaataaa gagtagttgt tcactaaagt cagaaaaatc aacttctaat cctacactga
     3601 caaaaaatac tggtataaat actgcatatc ctaaagcctc tacattgtgg tatacttctt
     3661 ctttaacctt tgtctgactg acagctatcc cagcaaaaaa agcaccaatg acagagctaa
     3721 gcccaacaag atccgctacg taagacattc ctaaacaaat aactagcgat acgatgataa
     3781 tagaagaatt tgcgtacact ttttctgcaa atgaaagtag gaaaggcgca atccatttga
     3841 ctaataagaa aataaaaaca aaataaatta gctgttcaat caaaacaagc ggtaaagaag
     3901 cattgctaga agcttccccc gttagaaaag ataggctgaa acttagaact aaaacgacta
     3961 aaatgtcatc taccacagaa gcacccaaaa ttgttgatcc ttcttttgta ttcactacgt
     4021 ttagttcttt taatacttct actgaaatac tgacagaagt tgccgcaaga ataatcccaa
     4081 agaaaatcgc ttcatttccc gaaacttgga atgctttgcc tgtcaaccag ccaaaaacaa
     4141 ccgggaagat gatcccaagc aaagcaacga acataccagg tcggaaatat tttttcaaaa
     4201 gagataaatc actttccaga ccagctaaaa acattaataa gatcacacca atctccgaaa
     4261 agtcatggac tagaatatca ggtttgaccc aattcaatcc agcttggcct agcaaaacac
     4321 caacaagtaa ttgtccaatc actgctggga tacctaatct ccgcgagata tgtgagccaa
     4381 tcgttgttga aatcaatatc aggcataaaa taccaataaa ttccattctc atcattcctt
     4441 tgtataaaat atagcccgaa cttagcataa catacattat tgaaaaaata ggaaaatata
     4501 ctcacaaaaa aaccaacaat ccaaaagatt tttttggatt gttggttttt atacggtcta
     4561 ttttaataac taaaaatgat taacgtatag tcatttttct tattctgatg aaattgataa
     4621 tctcctggat gcccatgaaa atcaaaataa agccgaatat ttggaataga agcatcaaac
     4681 ttttgaatgg attgaacaat aagacgaatc cgccaatcaa caacagaata ctactataaa
     4741 cactccaact tttagctttt gtattcatgc tgaagaacaa ctggaacaag ctgttgatga
     4801 taacagctaa tccaagaaga atcggcagga tagaaacgat tgcaggtgca aaagcataga
     4861 cgatcagagc taagatcagg aaaaaaagac cactgaacag accgatactc caagaacctg
     4921 ttttcctttt aaaacggtaa gcctcaaaaa gattcaacaa cccaagcaaa acaagatagg
     4981 cggtgatcag ataaccgata aaatgaaaaa cagcagaagg gtttagcaca atcgcaatcc
     5041 cagaaataat ataaacagca gctcttaata aggcgtattt ttgaatattt tgtttaatcg
     5101 tctcaaacat ataagaaaac ctcctttgac ttattttaac gtgaactagt cattttacca
     5161 aattaaatgt tacaaaatga gcgaaggaga agaatatctc tggaaaaatc aacttctgag
     5221 gatcttttcc atttttttgc cttttgctag ctcatcgatc attttatcta aataacgaat
     5281 ttctttcatc aagttatcct cgatgtcttc tactcgtaca ccacaaacaa cacctgtaat
     5341 caaggaacgt gaaggattgg ggacaggggc ttccgcaaaa aattgttcga aagtcacttg
     5401 tttttgtagt tgatattcta gttctgtttg ggaataaccc gtcagccaac aaatgatttc
     5461 atctacttcc tcttttatac gatcttttct ctccgctttt gctacataca taggataaac
     5521 gcttgcaaaa cttgtcgtaa aaatacgatt ttgcgccata atcagtcacc tttctttttt
     5581 tcaaattatt tactacttat agtatataag aaaaagagaa gaaaaagcga ttattcaaaa
     5641 aaacaaaggt tctataatat tttgtgttgg tggaaaatcg aaggcgattt tctactaaca
     5701 ctttttcttc gtttcaactc aaaaagagac tcacttttgg taaaatttag tcgaccaaaa
     5761 cgtcatccta ccaaaggagt
//
LOCUS       DARBDV010000132         5302 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000132 DARBDV010000000
VERSION     DARBDV010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.137"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..334)
                     /locus_tag="SCM56_002701"
     CDS             complement(<1..334)
                     /locus_tag="SCM56_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1111429.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIV"
     gene            complement(450..1364)
                     /locus_tag="SCM56_002702"
     CDS             complement(450..1364)
                     /locus_tag="SCM56_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HEG1111430.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            complement(1455..1883)
                     /locus_tag="SCM56_002703"
     CDS             complement(1455..1883)
                     /locus_tag="SCM56_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEG1111431.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            complement(1888..2262)
                     /locus_tag="SCM56_002704"
     CDS             complement(1888..2262)
                     /locus_tag="SCM56_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1111432.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            2548..2733
                     /locus_tag="SCM56_002705"
     CDS             2548..2733
                     /locus_tag="SCM56_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1111433.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            2745..2993
                     /locus_tag="SCM56_002706"
     CDS             2745..2993
                     /locus_tag="SCM56_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111434.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            3024..3203
                     /locus_tag="SCM56_002707"
     CDS             3024..3203
                     /locus_tag="SCM56_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111435.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(3246..3716)
                     /locus_tag="SCM56_002708"
     CDS             complement(3246..3716)
                     /locus_tag="SCM56_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111436.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            3803..4501
                     /locus_tag="SCM56_002709"
     CDS             3803..4501
                     /locus_tag="SCM56_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HEG1111437.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            4679..5020
                     /locus_tag="SCM56_002710"
     CDS             4679..5020
                     /locus_tag="SCM56_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111438.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            5013..>5302
                     /locus_tag="SCM56_002711"
     CDS             5013..>5302
                     /locus_tag="SCM56_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HEG1111439.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT         1762 a          828 c          956 g         1756 t
ORIGIN      
        1 aaacgatgta agtctcaggt atctttttct ttatagtgtt taagatatta ttcgtaggat
       61 aatatgtaga ctccttaact tgtcttttgt aaataactaa ccattcttcg acaagctcat
      121 gaaatgttag atcaggcttc tgaagtgttt tttcgtttaa ttttatatca atttttttat
      181 ttaactctag caatgccact ttctgagttt ctcgagattt atttttataa gtaatgctca
      241 ctttttttct ttttctagtt tttggatcaa tatatcgctc attatattta tatacttttt
      301 ttccgttttt atcagttttg gtttcaatcc acattgtttt catctcctat ttgttgctat
      361 aataggaatg gataagtaag ccaattatag caggtttatt tttcatcacg tccacaaact
      421 ttggcgaggg agggggcgtg tttttttgtt tagtagctac ctttccatga agtatctgcg
      481 aagaacactt caaatgttgt tccatcattt ttcacttcaa aatatgctgt tccttgtgcg
      541 gatttacccg gttggatggt ttccgaaaag taatctctac tagctaaatc agatttaata
      601 tctgatgaat cataaaattc taaataactt gaacttgcat caaaaggtgt agtccctgtg
      661 ttttctacaa gaaaatctac ctttgcgtaa aataggcctt ctggtttata gaaatcatca
      721 ccgtaatctt ttgatacaga ctggacagtt acgttaatag agttaccatt ttcagaagta
      781 aaaggaactg cttctcctat actatgtgca actgatgccg tctggggttc tgaagacgaa
      841 gttgtagttg tagttgtagt agagcttgaa tctttggatt ttccatcttt atctaagaaa
      901 gttcgttgat tataatatac aacctgttca gtttttggac taacccaaat taacattgtt
      961 tcatcatcag aatcagttga ataagtatat tggagcatct ctaatttttt tatatctttt
     1021 gcactatttc catgttcaat taattcagag tgctctttat aataatctcc ttgtgcatcc
     1081 tttgaaatag atacaattaa ttcatcccgt gtgatagcat tgtctaattc tttgtaaaca
     1141 aaatcatttg tccatttacg taacggtgaa ccaagtgtct gataaacttg agttaaatca
     1201 tcaccaactt ttattttatg aacgtctgat gaagttagtt tttttgtttc atattcagat
     1261 gttgatgaaa cagtattttc tttagtgcta gcagaattag gactagtgga gcatccgctt
     1321 aataccaagc ctaaaaaaag caacccaaaa acaatttttt tcatttttaa ttcctcactt
     1381 ctatgatatg atttttttgt aggatcttag aaataaggtt tttagtccgt gttgcagcac
     1441 ggactttttt tacattatat aagaaagcga taagcgcttt ctggaagtcc gtaaagattc
     1501 tttaattcct caattcgttt aggatattga tcattgtctt ctttgtatag agaaacaatg
     1561 agattagcag caaagcaatt agcttcgctt tcagatttgc ttctagatgt tcttgttgat
     1621 acatagtaac tggataagcc acgatgaaaa atagcgtgac ctaattcgtg agcgcaaatg
     1681 tagaatcttt cctcagagtc tctcagttca tcatttaaga agattattgc acgacctcta
     1741 atttcttgaa actgtccttt gggattctcg ataaaaggaa cgtattgaat tttaatgccc
     1801 atcttttcac aaatataaaa aggattagcg gactggtatt tccgcttcaa ctcctcgact
     1861 aaattaatcg tatccatctc cataagctca catctttttg cctttttctt tgtcttcttt
     1921 cacaatatcc cagaaggttc caataaggat atcttttacg cgttggacct gttcaggcgt
     1981 cagtgtttct ccgccataag acatattgac atttgaatct agtaatttat caagttcaac
     2041 tacttcatct tttgttgccc agtcaggaac ttggtttcta ccgagtaaat aatcagtagt
     2101 tacattaaaa taatctgcta caagttgtag tcttttagta ctaggagttc tatttttcca
     2161 ttgataaatc gtgtttttag gtatatttaa ttcttcttcc aattgagtta tacttatgcc
     2221 tcttttatga gctaattcct ttattctatc tagtaaattc attttcgcac ctcacaagct
     2281 acgaaaacac taataaaaaa attagttaaa ggtgttgaca actaataaaa gtgttagtat
     2341 actgttttcg taagctaaaa tattagctaa aaagacttga taactaataa acacttcacg
     2401 gtcggcaaac tctgaaatgt aaattactag gcatttctgt gtcttattta gctatgtcta
     2461 tatactaata aaaatattag ttattgtcaa cgaatattag ctaatttttt agcttacaga
     2521 ttattttttt agaaaggagc tatttttatg tctgagaatt tagacttaaa aattcgagcg
     2581 gagatgagaa aaagaagaat gactttcaaa gaactagctg cgcttatcgg tatttcagga
     2641 gcttatttat cagatattct aaacggcaat cgtgatggaa agaaagcaca acagcatatc
     2701 gaaacagtga aaaaaatatt ggacatccga taggggggta ggcgatgaca aaactaaaaa
     2761 aacaagattt tgtaaaaaaa tacaattatt ctccatctac ttatcaacgt cgaatgtcgg
     2821 aactaaaaaa tacagcaatt ttctcagcgg cgtatgaacg ggtcacagga caagaagttt
     2881 ggatcaatac agaattatac gataaatttt tgtctttcaa atcctataac aggttacgca
     2941 caagaaaggt aacacctaaa gaatttatcg agaagcattt agttgattta taaaaaacag
     3001 aacattttga gaggtgaagg ttaatgggta aattcaacag agcattggta ttcagcgcac
     3061 cgctaatcat ctacgcttta ggactttggg gaagcaggca agcgttgata ggaacgattg
     3121 tttacatggt ttggattttt atggggcttg atgaagctga agctgagtac agagcgaaaa
     3181 agccaaccga gggggctgac taaaaatgaa tagaaaagag aaactagaat gtatattact
     3241 attactcagt ttaattcttt cactaatttc tctattggga agtttgtatt tttgatatca
     3301 acaattttgt ggatttgggt tctatttaga gtaaatatca tttttgtttg gtgattaagg
     3361 agtttattaa aattaatttt ccctacttca aatgcaaggt aacaagatat agcactttta
     3421 ggaggaatat ttagcggtac agcttcagag tatattctat tatcatcgac tgatcctcct
     3481 ttagcaatta atttcttaaa tggagtagct gaccatctgc ggtttaagtt agcggatgaa
     3541 agttctaaat taactaacat agctggttct gttgaatagt tagaaatgat tacttttgta
     3601 taaatcatat ctgcagcgaa gtaagaagca ttaagttcaa cttctagttg agggcgcatt
     3661 ccttttattt ttatccctgt taaaaacgta ctcaatataa aacttattat tgacatccat
     3721 tgaaaaattg ttaagtgaaa aaaattcaaa ataaacacca ccagttttta actaaattat
     3781 accaaaaagg agagaagaaa taatgcaaga attagtaatt ttgaaaaata aagaagctgt
     3841 gactacgagc ttacaagtgg cagaaagctt cgagaaaaaa catcaacatg ttttaagaga
     3901 tattgatgca ctaaaaaaag atgtgtccaa ttttggacag atgtttgtag aaggtaatga
     3961 accagattca tatggcagaa atcgacgagt tttcttcatt agtagagatg gatttttctt
     4021 gctggctatg gggtttacag gaaagaaagc tatctacttc aaacaaaaat acattgaagc
     4081 attcaacgaa atggaagatg ttattcgcaa gaatactgtt cctcaaacaa ttgaagatat
     4141 gatgatctat caattagaag aaatgaaaga tgttaaaaaa gatgtttcca tgcttaaaga
     4201 tactatgcga attagcggac aacaagagtt tgaaattaag caaaaaggaa atatgaaagt
     4261 tatgggagtt ctagggggaa aagaaagccg agcttatgaa gaaatcagca aaaaagtatt
     4321 ctcaaaattt tggtctgaat ttaaacgtac cttttcaatc ccaagatatg gcgagttacc
     4381 tcgtaagaga ttcgatgatg ctgtttcatt tattgaaatg tggttgccag aaactgcgat
     4441 ccgtatggaa attgatcaac tgaacagaca acagagactt tttggtgatg acaatgaata
     4501 gagctgaagc gctaagaata gggacggcaa ttgctaattg ctggtggaaa tactataaac
     4561 caatcatcct aagccaacaa catattgaca agcaaaaagc atggcaacaa ataaaaaagt
     4621 gactccgccg gcaagcaaag agtcacaaag aaaacacatc ataaggagat tttagcatat
     4681 ggaaaaagaa ctttccactc tagatcaata tttgattgat cctgattggg gcaagccgaa
     4741 aattgaggaa acaagtggtc gaaaaatcag acgaaatctt ttgacgaatg aagaactagc
     4801 ttgggatcaa gatgatttag gcaaccatgt aactatttgg gatcatgttt atcttatcca
     4861 tctatcgaag cattcgaata aacctgaata tatttacgtc atcgaagatg gcttgattga
     4921 tgcgctagaa gagtacgaca gagataactt gattgatatc tcttattacg gaccaggtaa
     4981 gaaatacatt gctgaaatgg aggcagaatt tgatgagtga aatcaaaggg acaacgaact
     5041 ttgaaaaact ttttagtcgt aagttaaata aaattctcaa gaaaaaagga aattttgatt
     5101 atttatcttg ggctcacgcg tgggagatta tgaaaaagaa tgatccacag gcaacggtaa
     5161 ctattaatga gtacaaacac tacagggttg tttctggaac tcatcaagac tttcttgttg
     5221 aggaatataa accttttctt atggatgaaa ctgggactta tgtatctgtc tcagtaacgg
     5281 ttaaaggaca cacggaaacc ga
//
LOCUS       DARBDV010000133         5220 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000133 DARBDV010000000
VERSION     DARBDV010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.138"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..1256)
                     /locus_tag="SCM56_002712"
     CDS             complement(<1..1256)
                     /locus_tag="SCM56_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEG1111440.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
     gene            complement(1270..2487)
                     /locus_tag="SCM56_002713"
     CDS             complement(1270..2487)
                     /locus_tag="SCM56_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEG1111441.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(2477..4072)
                     /locus_tag="SCM56_002714"
     CDS             complement(2477..4072)
                     /locus_tag="SCM56_002714"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEG1111442.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            4197..4982
                     /locus_tag="SCM56_002715"
     CDS             4197..4982
                     /locus_tag="SCM56_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HEG1111443.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
BASE COUNT         1574 a         1051 c          862 g         1733 t
ORIGIN      
        1 actacaaaca cgatatgttg ccccattaat ttttggaagc gattggttcc ttttttctct
       61 tcctgtttct ttgcatattt caacgccgca tctagttttt gacgggttgt aatgatcaca
      121 acattcgcat tggcatctgc caacagagtt tcacttaact ctttggcact tcctgttccc
      181 acgatcaagc tattcgcttt ggcattacca gaagaaaccc cagtattaaa ctccgaagca
      241 aatttcgtaa actctgtggt ggtctgatta tctaaatctt tgcggtccac taacatgatc
      301 gttcgatcga tccccgattt tcgtgctaaa agcttagtag atacaaaact agtcaacgtc
      361 tttcctgaac cagtcgcatg ccacacatac cctgattgat gtttatttgc ggcaataaac
      421 aaggcttgaa tcgcatgcac ttgataagga tgtaacacca tgagggtttt attgtcttga
      481 tcttcactga caatcgtata atccgcaatc aagcgatggg catctgggat atttagcact
      541 tgtttgcaaa actcatataa gttttctacc tttttattat ctttcgttcg ccaactaaat
      601 aaaaacttag gatgcaaatc cttcggtaag gcatttgcaa aataccgagt cgtttgttca
      661 ttggaaacca caaacaactg taaggtcgaa aagatattgt tccgaaacat gccttcttca
      721 gcatattttt tgatttgatt aaacgcctga taaaaaccat cttttgcagt tacttgtttt
      781 agctcgattt ggataatcgg taagccattg atcaataagg tgacgtcaaa acgtcgatcc
      841 cgcgcttcta tgttacttcc acgttttgca atttgatgga ccacttcata agtagagatt
      901 cctccaccaa tgtcttgatt ggaataaagg ataagtgaga cagaacccaa ttgcggattt
      961 tcccgttcaa tcgtgatccg agccatccca ttttcacctt ttaaccattt cgcagcatca
     1021 aatggggttt tcgtccgtag caataattcg gttttgatcg tttcaaactc tttatcggtc
     1081 aaaggagaat cattcagttc tgcttgatta tttgagatga ttttttgacg taaattgctc
     1141 caaagatctt cttctgattt tagatccgga cgatacgtcc actgattatg tccttccccg
     1201 aggacacgaa tcaactgttc ttcgacttcc gcttcctcac ggtgactaat tttcattctt
     1261 cttccctccc taaacaaaca tcttttgcaa gaagcctttt ttcgtttcct tcagcaaatc
     1321 taacttacgt tgatgaagag cgatcgtatc atctagctgc ttgaagaagg caccaatttt
     1381 ttgttgttca tttatatccg gtggaaactg aaatgtcctt tgttctatac cacctttagt
     1441 cacatgtacc attgtcgttc cgttagttga ttctttaatt ttctctttat cagtcaatag
     1501 ccaagtatat agatactgtt tgtccaattc cttagataaa aatttaagtt tccaaatatg
     1561 ataatggtag attacacgtt cttcattcca catttcagga ccgaagcttg ttgcccataa
     1621 atataagaga tctcctttat tagcatactt gtcttcctct aattctaagt ttgaataata
     1681 ccatctatca ttagtattaa aattacctac tcttaaaacc ctatattttc cagaatcaag
     1741 taattcttgt tgtttaaaag ctctaccatt caaaaaaact accacttccc ccaacttccg
     1801 ctcttcccaa tctcccgtaa atcctggaaa acgaacttct ggcacttttg ctccgttttt
     1861 agggaacatt ttttgtaaga atcctttttt cgtttccttc agcaaatcta attcgtgctg
     1921 gtgaagcatg atggtgtgat caagttgttt gaaaaatgaa ccgatcttga tctgttcgtc
     1981 ataagttgga agtgagatag gtgtcttttt accactatca attgtatagg ttccgacggt
     2041 tccaatgcct gcaacgtcac ggatatactt ctgaattttg ttatttatga acgagtagta
     2101 aaagaagttt ccgtcaattt tatgctcatt cttgaaccaa atgatgtttc catccttaaa
     2161 gtaaattggg ttatcatcag tgattaataa ggggacacct attgatccga caccagttac
     2221 aagcaagtct ccttgagaaa ctttacctga atttttcgaa tattcattat atttttcttc
     2281 tgaaatgtaa agataatcgg atggttcctc attttttgca gctgagacaa tatcacgagc
     2341 acgtaaaaat ctaatacctg agttagtcca atctgattga tgaattcgct tcacagaagt
     2401 aacatccatc atatctccta acttacgctc ttcccaatct tccgtaaaac caggaaaccg
     2461 gatttctggt cgtgtatcat tgctcatgtt tgaacacctc cagcgcacct tgtaaccacg
     2521 cttcattttc aggtgttgtt tgcagggaag agatagcttc taaaagttct ttttctagca
     2581 cttgctgttc ttgtcgtatc ttcttgatgt catttccgac atgaaccata tcaattggtt
     2641 cttcttcttc gaaagtatcg acatagcgtg ggatatttaa gttatagtca ttttctttga
     2701 tttcatcaaa ggttgccaaa tgagcgtatt tctcgacatc ttttctttca gcgtaggttt
     2761 cgaggatttt ttgaatgttt tcttccgata atttattctg atttttccct ttaacaaatt
     2821 cacgactagc atcaatgaat aatacatctc gagtttgacg attcttcttc aatacaatca
     2881 ccgtcgttgg aatcgatgta ccaaagaaca agttcgcagg cataccgatc acagcataaa
     2941 tactgccgtc ttctaataat ttttgacgaa tgaccccttc tgcagcacca cggaataaaa
     3001 ccccgtgtgg caagacgata gccatagtcc ctgtttcctt caaatgatag aaaccatgaa
     3061 gaagaaacgc aaaatctgct ttggattttg gcgctaattt cccgtagcga ttaaaccgag
     3121 aatcatctaa gaaggtcgta tctgctgacc aattagccga ataaggtgga ttcatcacta
     3181 ctgcatcaaa tgtgtaaggt tcatctgttg gccagtcctt attcaacgta tccccattgc
     3241 ggagattcat ttcttcggca tctaccccat gtaaaatcaa gttcattttt gctagattgt
     3301 atgtcgttgt atttagttct tgcccatgat atttaacgtt atctggatga gttaagtagt
     3361 tacgtacatt taacatcaag gaacctgaac ccatggttgg gtcaaagaca ctaaagaatc
     3421 gtcgttcttt ttgatccaac gtcacgattt gtgccatcat atcagaaacc atatgaggcg
     3481 tgtaaaattc tccggctttc ttccctgctt ctgaagcaaa ttgactgatc agaaattcat
     3541 acgcatcccc gatcacatcc ccatcgtgac ctaaaacttc cacgtcattc aatttcttga
     3601 taacttcggt aatcgtcaca tttcgttgtt gctcatctgt tcctagtttt tttgattgga
     3661 gatccacatc atcaaataac ccattaaatt gattgtaggt agatgccaat tgcacaaagg
     3721 ctttgtttag atcgttcaat tggaacgttg cttgttttgc ttgatccgct aagacgttga
     3781 aaagatactc tggtgagatc gaatacccca aaatatcaac gattgtggca atcaggtctt
     3841 cttttgattc ttcatctgaa agtaattcac gatacaacat tgtttgtttg cttactgtgt
     3901 catattcctc taaggattca tctgctaaaa gaaccacttg ttccaacaaa cggtcggata
     3961 aatatttata gaaaatcagt cccaataaat aatttttata ttctgacgca tccattttac
     4021 tacggagatt atctgccgca ctaaataatt tttgatttaa ttctgcaccc aaaaagatac
     4081 actcctatct cttatttaaa gttgctatat accctcttat tttagcatac tcccttttag
     4141 aaaaagcaaa aaaaaaagaa aaaatcttat tcttgataac aagctacgtg acgtatatgc
     4201 ctaacaaaaa tagaactttt gaatcaatct taaatgaagg tttaaccaca gaaccggcat
     4261 ccattatttt tgatgaagtt atcgatgacg ttgaagatag taagtatcat aaaaatttaa
     4321 aagaactttt ttattttgca ctcaacaatg gaccaagttt gttcactggt atatcagtac
     4381 ctgatagtga taatccatta attatggcaa aaatttactt aacaaaatgg tgtaataaat
     4441 acatcaggga taggaataac ccagcattaa aaaaacctct taaaacattt ggcgaaaaag
     4501 atgctgcttt aactactaga gtagcagcaa atgctaatat tgatgatcaa aaagtattga
     4561 atgactactt gcgtggacat tttttatata tgagcgctga gaacatgaac ggaagtattt
     4621 tagaggaata tttagccgaa gtgttagaac ctgaaggttg gatttggtgc gccggctctg
     4681 tatatcgagc tgttgatttc tgttatttag gaacctcacc aattctttta caagtgaaaa
     4741 ataaatacaa tacagaaagc agttcgagtt cagctataag agtaggaaca acaattagaa
     4801 aatggaatcg tttaaataag tcgacaaaaa tttctggttt ggatagccct atccccaatt
     4861 ggaaagcatt aatagaaatg actgaagcta gtaaggaact ggcagctaag ttaactgaaa
     4921 atagttattt agcttatatt aatgaaaaat ccacacgaga attatggaca ctcgatgact
     4981 aacaatatcc ttattagtat ctatccacga tacatggaaa aaattataaa caaacaaaaa
     5041 aactttgaat ttagaaattt ctatcttacc tcagatcctt ataatatctt ttggatttac
     5101 gaaacaatcc ccaccaaagc tttaaagtat aaattgattg taaaaaatcc aattactaaa
     5161 cttaagaaaa atcaacacta ctttttagga gacgatgagt ttgaacaatt agtttaaaag
//
LOCUS       DARBDV010000134         5039 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000134 DARBDV010000000
VERSION     DARBDV010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.139"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            413..619
                     /locus_tag="SCM56_002716"
     CDS             413..619
                     /locus_tag="SCM56_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HEG1111444.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(821..1822)
                     /locus_tag="SCM56_002717"
     CDS             complement(821..1822)
                     /locus_tag="SCM56_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG1111445.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1827..3740)
                     /locus_tag="SCM56_002718"
     CDS             complement(1827..3740)
                     /locus_tag="SCM56_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEG1111446.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3908..4414)
                     /locus_tag="SCM56_002719"
     CDS             complement(3908..4414)
                     /locus_tag="SCM56_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEG1111447.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4574..5014
                     /locus_tag="SCM56_002720"
     CDS             4574..5014
                     /locus_tag="SCM56_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEG1111448.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
BASE COUNT         1391 a         1008 c          769 g         1871 t
ORIGIN      
        1 tatttttgtt tcttattttc taaaaaatta aaaaaaattt tggtgaatta agtatttcct
       61 tgataacact ggcttttggc ctcattttag gttatcattt gtaattttat ttcacagttg
      121 atatgattgt tatgtaaaat aaaaaagttt aggatacatc gaatttttgg ggaaattctt
      181 ttgggggtat cctaaatctt ttctattata ttcccatatt ttctattatt ggtctagacg
      241 tatgatggta aactttgtac ctatctgatt gctgaatcat taattgctta tcattagcca
      301 tctttgtttc aagttcgcta gatgatcatt ttattcacat cctaacaaac ttgtcacatt
      361 tatagattct taatctattt atcatacaat tataaaaaag gaggtgctgc ttatgaaagc
      421 tatccatatt tctttgcttt ttttcgctag tgcattgatt ttatttgcta ttcatctttg
      481 ttcccagcct tatatcaata acaacggaat attagtggaa ccagcattct tttgcttacc
      541 actcggcttt ttgtcccttc ttgcctccgc aggcttcgga tttgttgctt tttttaaacg
      601 atcaaaacaa caaatctgac ttataaaatt tacgtaaaag agatcgtgac aataatcgtg
      661 tgatattccc aaatttcgaa taaaaggtgc cccttgccat ctctaactcc tcagtcacaa
      721 gttacaatat tcataaacca tgaaaagctt gtttattgaa attgctcctt gtgacttgga
      781 gctaagtgac agattacaac ctcttttgca tcataagact ttactccttg aaaaacatta
      841 ctaacttttg atatgtttct tgcgcttgtt tattgctgaa atcaaactga tattcatggt
      901 tcaaaacagc atttttctcc gcataatcta cttttgtgac tttcaccccc gctttctcaa
      961 atacagaagt tacttcttcc atttgtttcg taaacgtatt tgtatttcca tccgttaaga
     1021 atgtgttttg cgtccaacga tccgcatgtc tgaccaaggc taaacttttg accttctctg
     1081 attgttcaaa gtccttatta tcaaagtaag cccatccaca tttttcataa agaaagctag
     1141 agattttatc acttacttga gtaagtgcag aaggatccaa cagaccagac aaagaaataa
     1201 acttcgtaat ctgcttgttc tccaaagtcg gatcaagatg atcagctttt ttaatttctt
     1261 cattttgaag tgctaacaaa tattctccac taatttgtgc acctgccgaa tttccaccaa
     1321 tcgaaatatt cgaccaatct gcttgatctt ttatctcttt tttagctgct tgaatggctt
     1381 cgttcaattg gataactggt gtaggatgtt ttgcttccgg tgatcgttca taatctatat
     1441 ttacgatgat ttgctgcgtg tctgaacata gatattccaa ataatcacga caatcatttt
     1501 tatcccctcc tacatatgcg cctccatgca tccagaaaag aatcggtaat tttttctttt
     1561 ccactttcgg tgtgtagata tccatattat tatttttgta ctttgaagga taggatattt
     1621 ctttttttat atcgatttgt tcgatttttt gctggaaatc atctggtcgg gcatatactt
     1681 gttctttttc acttgtgtca aattgttttc taacaagcaa gctaatcggc tttggtgtga
     1741 tttgattaat caaaaataca gtgactaaaa taactacgat gataactggt actgcaatca
     1801 ctattcgctt gaaccatttc attcatttac cctctctttc ttcgccaaat ccatgtgata
     1861 atcgtgctaa cagataaaaa tccacctgaa taaatgagaa gcgaatgttt cttttctcca
     1921 gttttcggca acattctttt ttttgcatct gtcttttttt gtactttcac tgtcatctga
     1981 atcggttgcg tatcatcact tgattgatcc tctcgaggtt cattctttcc cttattgtct
     2041 gcattctctg tcggatcttg cttttttggt ggagctactg ggattttttt gtaatagaaa
     2101 ataatagttg gtatcgtctc attaaaaatc ccttcagtcg gtccttcaac atttgctaat
     2161 tcgtatcctt cgaatgtctt ttgctctgtc atataatttt ctccataagc tccgtaaagc
     2221 tggatttcgt cctggtcttt gagtagactt ttattgtttt catccacata ctttacgttt
     2281 atctgatcat ggaacactac tttcaaaaag tccgttacat gtttcgtgta ggcagcatgg
     2341 tcaacttcct gtgcctttcc atgccctgct ccttctacta ataaaagatc tttataccct
     2401 cctgataact catataattt ttgcgcgtta gacacaggta ctacatcatc tgctgttcca
     2461 tggataatca atttaggcaa tgaatctgac attccaatcg taggtagagc agcttttgta
     2521 tccaaatgca tctcctgatc aaaatagtat tgtttcaaaa ggcccataaa taaatcttca
     2581 tgctcttgat ccccaatgat gtctaactga ttttttatgg catccggtag tgcgggggct
     2641 actgtctggc tgtacgtaaa acgtagttgt tcatcaattg atgcaaatcc gcaattttca
     2701 ataattcctt tgacattgac aggaatatct ttcgctaata cagacatcat cgtagctgct
     2761 cccatcgaac caccataaag taaaatcttt tgagatggcc gctctcgaac ttcttgattt
     2821 atccagtaaa ggacgtcatc tgactcatat tgtccatagg tcacataatc gcctccgcta
     2881 ttaccagtcg ctctggaatc aacaaataat aaattatacc cggccttata aaaatcattg
     2941 tattcttctg taacgatccc gttattccaa ttccctcgaa atccgccatg gatcatgact
     3001 gttttatctg aaccttgatc gacataatat gcatatacat ccatcatttt gccatcttgt
     3061 ttgtttagta cagtttgatg aagttgtttt gtatttggat agaagcctgt gttttctgta
     3121 ggcgttgtcc catcatctgt acctccaagc atcgacatca acacactgtt ttttgcaaaa
     3181 gcgatatcgt acaatagata agtagcaatc agatgtttga taatctcttt atctacacca
     3241 taatcattaa cagcaagctc agtgactaat aactctatta attttgactt gatgtcttct
     3301 gttggtccat taagtatttc tgtcatctgt ttaccgaagt aattcaaata ttctttacca
     3361 ttgaaatttt cacctttacc gatatcatca ttttctgtcc cattctcttt gtcacttact
     3421 ttagcagaag aggattcgtc gtttgacatt tttgtagctg ttgcttcttc tgtttgttgg
     3481 ttatctgtgg tagcggtcgt ttcttgatct gtgctagtaa cagatgtctc aaccgttgtt
     3541 tcagtatttg tttctgtttg tgaagtactc gatttcgttt catttccagc atcagtagtt
     3601 tctgtggttt ctacctcccg agattccgat tcggaagaaa tgctctctcc atatactgtc
     3661 cattgcggga cgcttgtact tacagcaaat aagatgacca acgctccctt taatacttgt
     3721 tttgttctca agctattcac tcctttttat gtttctagga gaattatatg taaacgcaaa
     3781 cattttttat acaaaaaata aatggtttat cccataaaaa tagaacagga ttattttctc
     3841 attttacgta ataaagcaga atggataaac cagtatccat tctgcttttt ttaaacgaag
     3901 aaacaactta ttgctccaaa aatgtcagaa atcgtttatc ccaaattttc tcgtaaaaag
     3961 cgattgtttc ctttgctttc ataaaaggat tatcataagt tgtcgttgct cctctacaca
     4021 tcataagctg gtatcctaat ccatgagcaa acttcacagt cgtatctata caatactctg
     4081 cttgtgcccc acataattcg atcgaatgac agtccaattc agttaataaa acactcaaat
     4141 tagtcttata aaatgaattc gcatgggttt ttctcacaaa ataactgtgc tcaggaatag
     4201 ataattccga caatagctcc catggttttg tttgtggcac taattgagca tcttcgtgtt
     4261 gaacgaaaat aactggtttc ccaatcgttt cgtatgtttt gattctttta ttcactaatg
     4321 ctgtcactgt ttctaattcg tgtatcggcg aagaatcaaa acatacccct ttttgtaaat
     4381 cgatcactac tattgcgtcg gctgtgtttg acatacttgt gccttctttc ttttttcttg
     4441 atttaatagt atttcttatc tagctagttc cattatacgt gacaacattt tcttatttta
     4501 cacctctaaa tggcactttt cgactatttc ttcatgttat tatttcataa atacttagct
     4561 attggaggaa tgtatgatga ttaaattgta tacaggttct ggctgctcgt cttcaagacg
     4621 cgcaaaacgt tggcttatcg tccatgatat accgtttatc gaacagaact taattcgtca
     4681 accactaacc aaaaatgaat ttatccactt actaaaattg acaaacaacg gcgtgaccga
     4741 aatgctctct actcattcta ttagttataa aaatcttgtt cacagaatag atgaactcag
     4801 tttgagtgaa ctatatattg agattcgtac atccccttct ttattgaaaa aaccgattat
     4861 ctacgaccaa tctgcaaaac ttttgactgg atttagcgaa gataaaatca ggatgttcct
     4921 ttctaaaact aaaagaaagc aagagctcaa cgaactcttg ctttctggtc atcttgtcga
     4981 ttttaaagaa aataatcttt ttgcaatgtc ttaattttgg cataaacaat ctgcttttc
//
LOCUS       DARBDV010000135         5027 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000135 DARBDV010000000
VERSION     DARBDV010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.140"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(198..428)
                     /locus_tag="SCM56_002721"
     CDS             complement(198..428)
                     /locus_tag="SCM56_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111449.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(522..860)
                     /locus_tag="SCM56_002722"
     CDS             complement(522..860)
                     /locus_tag="SCM56_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111450.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            complement(1788..1904)
                     /locus_tag="SCM56_002723"
     CDS             complement(1788..1904)
                     /locus_tag="SCM56_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEG1111451.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            complement(2380..3258)
                     /locus_tag="SCM56_002724"
     CDS             complement(2380..3258)
                     /locus_tag="SCM56_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HEG1111452.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            complement(3581..4606)
                     /locus_tag="SCM56_002725"
     CDS             complement(3581..4606)
                     /locus_tag="SCM56_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEG1111453.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
BASE COUNT         1689 a          924 c          759 g         1655 t
ORIGIN      
        1 agaaacaaac tttagaaact ctagaagaac ttctgaattt atatagaagt gtgtagctac
       61 ttactaaata tacaaaagtt aaaataaaat actctggata ctctttatac tgcattaaag
      121 ttagtaatat taacagcaaa gatatcgaaa aaacaataac ataatatgtg tcaaaatttt
      181 tcataataag aactccctta ctgttgcata ctaacataat ccaaaaactc ttctacttca
      241 gctaaagaca ttccactttc tttagtccaa aaaacagttt tttctatcga tccttctctg
      301 taaactgtaa atgtcggagt cgagttaatt gagtagcgac tcttgaattc tttccactca
      361 ttttcatctt ctctaacttt tgctacattc acgtaataaa tattttcttt tcccttttct
      421 accagcatat cgaatagctt cctgcttttg atgatcgatt tcttgtgcta catctagtaa
      481 attttcatca atttctatat cataaagtat ttcactagtg tctacatcca caatatatac
      541 tttttatcag tagcaatttt tgagtaatcc gttaaaatga tctcagtctc gtcactttca
      601 tcagtataaa tatacgttaa caatgaactc accttttcaa tcggtgttag ctctaagcct
      661 actgtatttt ttactattga gtcattattt accaccataa cttcgtactg tatataccat
      721 cctatttggt ttccatcaat atcagtaatt ggctcactag ctaatggagt ctccggaaca
      781 atattttcct gatatttctt aaactcttct gtagcacgaa aaaggttatc atgttcctca
      841 cttgaaactg gttgagtcat agaaaatgtt acttggctaa acagagggaa aataaacact
      901 acaatcaaca aagtataaaa aaaagccctt ctttttcatt ttttatcttc caatcacaaa
      961 ttgaaaaact tattcctgca actttgagtc tagtatcaca atcgactgac tacaactatt
     1021 catttacatt ctactttaag ttcctcctta accattaatt ttattaaaga tacttgtaat
     1081 agtgattctc acaaaatttc atgaaaaata ctttattact gtatctaaaa ttccaataca
     1141 caacttatcg actattatct gaaaaccaaa acttattatt ccttgatgtt attctagcaa
     1201 tagtaactgc ttttttattt ttttgcatat tttttcggcg ttacttttta aagttaactt
     1261 tttcgacaaa tagaaagaga tacgaatcac tgattttttt gaagaaaaga aatggagtga
     1321 gctgaacgtt agagatattg ggagctttcg taactgctta ttaaacgata atggttattt
     1381 gcaaccgtat tgctcgcttc tacatgaaat atttcatgaa atatttcgca aaacctttga
     1441 ttatgtagtt acgtttccat tcttgactaa aaatgtttca aaaacgaaca aaggtgttaa
     1501 aaggtaaagt gatcaatact actggtcaaa ataagaggtt gaaagggaat tgaaaaataa
     1561 ctacagataa tacttttata aacgtacgtg ctttgtggtt ctgtagttaa ctatataact
     1621 ggaattaggg aatggtatca atttgaaaag aaagattcta ccaatttatc atatgttaaa
     1681 aatggaaaat caacagcagt tctttcggaa accttacaca aaaaccgatt attgaaaata
     1741 gccgaaaaac gtttttcggc tattttttta attaagtttc cttcttctta ccttttccca
     1801 ctattccgtt tcacatcttt gatgatcttc agcgcacgtt ttcgtgtttt gatatttggg
     1861 cttttcaagc ggcggtatgc acttgagata tctgtttttt ccatatggac gacccacttt
     1921 cttctttgat tgtagtcatg ctaaaaaaca caaatcgaaa caattacgat ttgtgtttta
     1981 aatggtatca aaagttaaaa tgaaagtcaa cttgttaaaa cagtcgatca gactagctga
     2041 ttgactccta tctctggaaa taaattttca ttattacgtt ttagctacct aaaaaaggcc
     2101 ctcttgtccg ttaactttaa tatctattgt tatagctagt ttacctattg aacgtactgt
     2161 gtgttcgata gaacactaaa aaagaggctg ggacagaagc gtttaactcc aagaaataag
     2221 aagaaattca cgaaaattgc ttttcaaatt tttgtgaatt tcagcttatt tccgaaggag
     2281 ttgcttctgc ttccgccgtt tatacgtgtt tagagcgtga gacaaaagtg ttctttactt
     2341 ttgtctcacg ctcttttaaa ctatttcgaa gtactaaact tatgaagtaa gaatacctaa
     2401 gccagagcca atgtgctcgc ctgcaagcaa ttgctctttt gcttgtcgct gtaaactcgt
     2461 actttctgga taatcatgta tttgggcatc tactcgtcca aagatccaag ggactccact
     2521 atcgtcctgt tcatcaactg ataataatcg ataatcggct gcaaaaagac agatttttga
     2581 ctcttttttc aaccatttag taagattggg tgatagtaac caagtatctg tgtacatcac
     2641 ttggtaatgg taatcaggaa aataaagttc aaaaaaacga atggcacttt cgtaactatc
     2701 tcttatctta ttctgagata aatctgcatc ggaaggaata tgaagagaaa ggcactgctt
     2761 tccttctaat cttagatccg tgtacccttt cggaaaatat gtcatttcgt actccaattc
     2821 accaatccga aagatcaagc cactagtgta atgccaaatc caaaaaccac ggtcaaagcg
     2881 atagcttcct gttacttgga aatgttcatg aataaatcta gtaaaagcat tcattgttgc
     2941 caaatagata gttcttggaa tcttcaatgg ttgataaatt tttttccaac tttgagaagc
     3001 tgcatataga taaatagcta atgctgcaaa aggttcctct tcatagactt tgtctatttt
     3061 ctttgcagtc gcctttgctt tattaggatc cattagttca tgactatatt gctcaaacac
     3121 ctcatcaagg atgttttttg cttgtacaat catttgctct ataacgactt tgggaagagt
     3181 taaagtttct gccagaattt ggatttcatt gcttattttc ttcatcaaat ttcccctttt
     3241 catatctttg aacctcattg tttaaatgac tgattatccg gacaccatat ggtataaaca
     3301 tacttttatt gtacactaac tataaaactt aaactttcta tgaagaaaga atatctgtaa
     3361 gactttcaag gaaagcattg accatctcag gaaagtttat tcagttacca tcataaacaa
     3421 aattttttga tagaataaat ataaaatttt tttgaaagtt cctttccaca tttttgtgtg
     3481 aatttgactt aatgctgaga atctggcttt tgaacacctt gaattagagg ttgtgacaaa
     3541 aatcttgtca taacctcttt tggcacatat atttgtcctt ctattctaat ttttgacgaa
     3601 caaccgttaa ataatgttgc aaaccaaccg tcattaccca cgcaacacct aaaccgatga
     3661 aacttcctat gacgatccct aaaaaacgat attcgatcaa agaaatcaag tatgctccac
     3721 gaataagtgt agatagcatc aacgccatcg gattaagaaa aatattagca actgcataat
     3781 ttctagcaac tagaaattgt gcgattccat atagaaaagt gatcagaaaa atggtctgca
     3841 actgcgtaaa cggcacaaat gacaagaatg ccgacacaca taacccaccc atcgatccaa
     3901 tcagatattg gacttgtcga tgcttcatcg catccaagtt ttctgctagc aaaatcgatg
     3961 cacatgacaa ggtcaaccaa taaggattat gcagattcaa gccgtgactg acataaacgg
     4021 aaagaaataa ggcagcgctg tagaaaacag aatcaatgat cactaaaggc tcttcgtgaa
     4081 atctttcttt taaagttgct ttttcaattg tctgctcagg ccgtgaatcc agtaattttg
     4141 taatcactgc cgcaatcagg gcaaaaacga cacccataaa aaaataaagt gagacgatcg
     4201 gtaattgagc gattggcagc tgcatgctcg ttcccatggc agaaagcatc gcaaaaaaca
     4261 agccacctgg tttagaaata tggaataatc gcaacacgaa tctgctggaa aaagcaacca
     4321 aagctacggc aaatggttca agccataaaa cataagtcga taagatcccc aaaacgaaag
     4381 caaccaacag tccgcaaccg atcagaaaca tacgtttcag caaatatttc atcggtatgt
     4441 tttgataata gaaaaaagta aagatcccta atgatgaaaa acttgaaatc aaaaaatcat
     4501 gatttatgta acctaaccat aatacaataa agataagtag catcagattc atgatacgaa
     4561 aaggattatc ttgtatttta ttgaaacgaa aataagtctt gaacaatcta tccctccttc
     4621 ctagtaattg gaaaaaaagc tgaaccaaga tgaaatgcca atcccgtttg tcagcattac
     4681 ctagtataac aactatttct tgatttttca taaacttagt atcaaaaagt aaacaacttg
     4741 gagaaattag aacaaaaaac gaacattccg agtcgttttg acagaaaaaa gatagcaact
     4801 tttccaagca actcgctatc ttagctttgt taaagaaacc ggattcccaa caaaaaaaag
     4861 cttctaattt tcgtacgata actacgttgg tccttaaact tcatactttt ttatgatttt
     4921 tcagcggttt tcgatggtat actttttaaa tggaggagat gtctatgcgg tatgtatcaa
     4981 aatttttatt agggatttat ttattctttc tattatggtt gatttta
//
LOCUS       DARBDV010000136         4893 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000136 DARBDV010000000
VERSION     DARBDV010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4893
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.141"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..563)
                     /locus_tag="SCM56_002726"
     CDS             complement(<1..563)
                     /locus_tag="SCM56_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HEG1111454.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="SCM56_002727"
     CDS             complement(750..1400)
                     /locus_tag="SCM56_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111455.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="SCM56_002728"
     CDS             complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="SCM56_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEG1111456.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2912..3910)
                     /locus_tag="SCM56_002729"
     CDS             complement(2912..3910)
                     /locus_tag="SCM56_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEG1111457.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="SCM56_002730"
     CDS             complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="SCM56_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEG1111458.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
BASE COUNT         1486 a         1051 c          757 g         1599 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg gattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt
     2041 cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa
     2101 gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt
     2161 gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat
     2221 tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg
     2281 ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat
     2341 agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa
     2401 tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg
     2461 gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg
     2521 aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa
     2581 gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt
     2641 ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa
     2701 gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct
     2761 ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat
     2821 gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc
     2881 tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga
     2941 acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa
     3001 agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt
     3061 gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat
     3121 tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat
     3181 cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg
     3241 tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga
     3301 tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa
     3361 cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt
     3421 caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa
     3481 aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca
     3541 tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg
     3601 caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt
     3661 attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag acatttctca
     3721 agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat
     3781 ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa
     3841 ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt
     3901 tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt
     3961 gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg
     4021 ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc
     4081 aggcaataca gtttccagaa gatccaaaaa tttatggtag aatgattgcg tttcatcaaa
     4141 ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat
     4201 ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc
     4261 gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa
     4321 tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac
     4381 ggaagcttgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat
     4441 caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc
     4501 ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc
     4561 gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc
     4621 catttttgtg actttacctg attctttgat caattcccag aattttggaa tcaatcgagg
     4681 cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt
     4741 ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa
     4801 cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc
     4861 tttttctttt acaaaaagat ttggaccaaa tat
//
LOCUS       DARBDV010000137         4798 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000137 DARBDV010000000
VERSION     DARBDV010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.143"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..1097)
                     /locus_tag="SCM56_002731"
     CDS             complement(<1..1097)
                     /locus_tag="SCM56_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEG1111459.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENA"
     gene            complement(1227..2603)
                     /locus_tag="SCM56_002732"
     CDS             complement(1227..2603)
                     /locus_tag="SCM56_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1111460.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2603..3259)
                     /locus_tag="SCM56_002733"
     CDS             complement(2603..3259)
                     /locus_tag="SCM56_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1111461.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(3269..4546)
                     /locus_tag="SCM56_002734"
     CDS             complement(3269..4546)
                     /locus_tag="SCM56_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1111462.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            complement(4565..4726)
                     /locus_tag="SCM56_002735"
     CDS             complement(4565..4726)
                     /locus_tag="SCM56_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEG1111463.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
BASE COUNT         1510 a          869 c          582 g         1837 t
ORIGIN      
        1 gcattctcat tagagaacac tccctttttt gtgacttttc tgaagctaga gtgaatactt
       61 tcaactgcat tagtagtgta catgattttt ctaatggcgc tgccataatc aaagagttgt
      121 tcaacatgag aaaagtttcg tttgcataca tctactgcac cagaataatg agaccaccga
      181 ttttgaaaac tgtcgaaggc agcgtgtgca gcgtttaaag aagaagcact gtagaatttt
      241 ttcatgtctc gacagacttc cttataatcc ttacttggga ggtatcgtaa cgcatttcga
      301 acaagatgaa caatacagcg ctgcacaatg acagaaggaa aaattgcttt tgcgccttct
      361 ttgagaccgg aaacaccgtc catcgaaatg aaaaaaacat cttctacacc acctactttc
      421 agttcatcaa atacttgcat ccagcgattt ttagattctg tttgattgag ccataggcct
      481 aaaatctcct tgtttccttt aagatcatag ccaagaatgg tgtacacagc atattcttta
      541 gcttcatagt tttcgcgtaa agtgacgtac atacaatcaa cgaatagaaa tgcataacac
      601 ttggctaatg gccgagcttg ccattcttcc aattcaggaa ggacggcatc cgtgatatca
      661 gaaatcattt cgtgggaaat atcaaagccg taaatatctt caacggttgc ggcgatatcg
      721 cattgactca ttcctcgtgc atacatggaa agaaccttac cttcgatgtc ggagacatct
      781 cgttttctat taggaatcaa ctcaggttca aaagaagcct cccggtctct gggaacatca
      841 atgtttactt taccaaagct cgttttaagc gtcttattgc cataaccgtt tcgtcgattg
      901 tcatgtttct ttggctcttt agaatgggcg tcatacccta aatggttatt caattctcct
      961 tgaagcattt tttcaaataa gggaccaaac acatctttta aaacatcttg catatcctct
     1021 acagattcgg gttgataagt attcaaaatg gattcagcta acttctcagc atcaggattt
     1081 cttttctttc tagccatcgt gtaatcacct cttgatctta ttttagaaaa ataaaaaccg
     1141 tgaagcaacc acccgcctag gattgatggt tacttacacg gtttatatta cactctcatt
     1201 tttggtgcta aaaagcaccg aaaattttaa ttattgtcaa ttaataattc ttttggattc
     1261 cttcttaata tattgaatga agaaataatc aatgatacaa gaagtactaa actcataaat
     1321 ataactacat aaatcatgaa tttaggtaat acattgatat ctaaacttga taatgtcttg
     1381 ttaaatcctt cagcttctgc cccaccacct aattggctag atgcagattg tttagctatt
     1441 tgtttagcta tatcaccagt aaccttattc aatatattat tcccaagctt gtcagctgta
     1501 tattgagcta agaaatagga accaacgaat gcggggattg atataaatac catctcaatt
     1561 aggaattgac caaaaatttc taattttgat atacctaatg atagtaatac tgctatttct
     1621 ttctttctag cattcatcca taagaataat aataatgaaa ctacaactcc tgcaaatatt
     1681 aatgagccaa aaaataattt atttgaaatt gagtaaatac ctgatataga ttgttgtaat
     1741 gcagggtaat ttgatgaact tttaatcaaa ttatattctc tccaatttat atctaatttc
     1801 ccaagatctt ttattacact atcaagattc ttatcacctt ttacaaagaa tgttgcatct
     1861 tggtatacag ctgtatcttc ggtattacca taaactttag cagctgtatg aatatcagtg
     1921 attaatgtat tttcgtatag ttcttgtgct gcacttactc caccgctatt atgcccatcg
     1981 aatagacctt taatttctac ttctactgtt tcatctgcac ctttctcatt atcagcatca
     2041 aataaattag attttatttt tatcttatct ccaactttaa ggttattttt ttcagctaaa
     2101 tctttatgca ttaagatttt atttttatct tcattttcta aatgctttcc ttctactaat
     2161 ttatatgctt ctgatacaaa ttttgtttct tttgttgagt cgttaacccc agttaacata
     2221 actgttcttt taaaattctt tgctctttca ggtgattgat tcgcaagagt ctcttgagtt
     2281 tcaataatat cataatcaac taaatctgca acactgttta ttctttttac ataggagtct
     2341 atactatctg tttgagatat ctttttaata tcttcacctt ttacatttcc cccacctcta
     2401 ggtgttcctg gatttacttg tctatttatc tccatagaaa aactatttgt tatattagca
     2461 aatgtttctt ttgaagctct gtccgtagca tctttaatag ataaactaat gagacttaaa
     2521 gttgacatag ataaaataac taatatgata attaatgatt ttaaactttt tctagttaca
     2581 taagcaaatg cattttttat cattattcaa cctccaaatt cattttattt acttttttca
     2641 actttttacc acttagttct aaaatgatat ccgcagaatc tgctacttcc ttactatgtg
     2701 ttacaactat tacacattta tttctatctt tagctaatgt ctttaatata ttaattattt
     2761 ctccagcagt aacactgtct agattaccag taggctcatc agctagtatt attggagcat
     2821 ctgataccaa tgctctagca atagctactc tttgttgctg tccaccagat aatttcataa
     2881 catttctttt tatttgtttt ttatctaaac ctagttcaaa caagatactt tcatctgctg
     2941 atttatttac taatctaata ttttcaattg gtgataaata atctattaaa ttatagtttt
     3001 gaaatactaa agatatatta ttttttctat gattactata tcctttattt tgtatatctt
     3061 cattcttaaa caatattttt cctgtttgag gtttatcaag tccagcaagt aaggaaagaa
     3121 gtgtagattt tcctgttcct gactttccta ctatcgcata aaacttccca agttcaaatt
     3181 tttgatttac tcctgacaaa actttttctt tagaatttgc ataactatat gctacattct
     3241 ttatttctaa tatatccatt attttctcct aacttatttt tgataagatt tctttaggct
     3301 tcttgattaa cattaatgaa gaagcaaata caactgataa aacaataata cttattaata
     3361 ttaaataact ttgtccaagt gttgttatat ttgacataaa actactatta tttattaaac
     3421 ttccaccgga aatcattgaa tcctctgagt taatgaatcc atctacaatt acttttagta
     3481 atacatttcc taaaaataag gaagatatta tacttggtat cgatatgaat attaactcga
     3541 atataaattg cattataatt tgtatcttag atgttccaat agataagaat atacctattt
     3601 cataaattct ttcccttaac catagaatca agattaatga aagaacaacc atcccaccta
     3661 acataataga ataagtcatt acttttatta tatattttat tccactcact gactctaaag
     3721 actcttcaaa cgcattagaa tctttttcaa caaaatactt tgactcatca attttaagct
     3781 ctttcaattt gtttaaggct aagtctgtag attctgcact accagaatac attaaaattt
     3841 tatttgcaat ttcattattt tctgaattat ttaatatctc ttggcttgtt gaataatcta
     3901 caaacaccat attttcacta aaatcagaag ataatcctgt atatgtttcc tgttttttac
     3961 cagaaaagat ccctataatt ttaaatttat gactttttat ttttccactt ttttcaatat
     4021 ctagtaattc aagatcaact tcatcaccta attttagatt gttttgttta gcaaattctt
     4081 catgaacaat aattgaattc ttatcatttt cttctatatt tttaccttct ttaattgtaa
     4141 atactccact actaaataaa atatttcttt tagtattatt tgtagcttcg agtgaaacaa
     4201 cattcttaaa ttcgtcagat aaatcttctc tatttattct ttgttctccg ctaactactt
     4261 tagcatcttt tagttttgct aatccatcat attgaattat tttttcttca atttctttta
     4321 atttttcaat atctttaaat tgattaacat taaaatattt accatctttt tttgttattg
     4381 atattgaaga attagaactt tcatataaag ccttttctat ttcattgctt gatttcatta
     4441 ttgttaaaca agaatacaag caagaaagaa ctatagttaa tatgataaaa attattaaag
     4501 ttctattttt ctttcttgta atatatgcta tagcattttt tatcactttc ctaactactc
     4561 ctctctattt tcttactcca taactaattg catccactgg acaagcattt ttacatcgtc
     4621 cacatctgat acactccaga tgattgcaat tctcaactga atctttattc atttggcaaa
     4681 cttttttaca ttttccacaa ttaatacact tatccttatc ctgaatcggc tacactagac
     4741 tagacagaaa aaataaggtg tgtagaataa taaaaaacac actggaggat ttttcatg
//
LOCUS       DARBDV010000138         4798 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000138 DARBDV010000000
VERSION     DARBDV010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.144"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            51..746
                     /gene="vanR"
                     /locus_tag="SCM56_002736"
     CDS             51..746
                     /gene="vanR"
                     /locus_tag="SCM56_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HEG1111464.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            724..1878
                     /locus_tag="SCM56_002737"
     CDS             724..1878
                     /locus_tag="SCM56_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HEG1111465.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2093..3061
                     /locus_tag="SCM56_002738"
     CDS             2093..3061
                     /locus_tag="SCM56_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HEG1111466.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3054..4085
                     /gene="vanA"
                     /locus_tag="SCM56_002739"
     CDS             3054..4085
                     /gene="vanA"
                     /locus_tag="SCM56_002739"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HEG1111467.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4091..4699
                     /locus_tag="SCM56_002740"
     CDS             4091..4699
                     /locus_tag="SCM56_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HEG1111468.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1482 a          919 c         1122 g         1275 t
ORIGIN      
        1 tataatctga taaattatta agggcaaact tatgtgaaag ggtgataact atgagcgata
       61 aaatacttat tgtggatgat gaacatgaaa ttgccgattt ggttgaatta tacttaaaaa
      121 acgagaatta tacggttttc aaatactata ccgccaaaga agcattggaa tgtatagaca
      181 agtctgagat tgaccttgcc atattggaca tcatgcttcc cggcacaagc ggccttacta
      241 tctgtcaaaa aataagggac aagcacacct atccgattat catgctgacc gggaaagata
      301 cagaggtaga taaaattaca gggttaacaa tcggcgcgga tgattatata acgaagccct
      361 ttcgcccact ggagttaatt gctcgggtaa aggcccagtt gcgccgatac aaaaaattca
      421 gtggagtaaa ggagcagaac gaaaatgtta tcgtccactc cggccttgtc attaatgtta
      481 acacccatga gtgttatctg aacgagaagc agttatccct tactcccacc gagttttcaa
      541 tactgcgaat cctctgtgaa aacaagggga atgtggttag ctccgagctg ctatttcatg
      601 agatatgggg cgacgaatat ttcagcaaga gcaacaacac catcaccgtg catatccggc
      661 atttgcgcga aaaaatgaac gacaccattg ataatccgaa atatataaaa acggtatggg
      721 gggttggtta taaaattgaa aaataaaaaa aacgactatt ccaaactaga acgaaaactt
      781 tacatgtata tcgttgcaat tgttgtggta gcaattgtat tcgtgttgta tattcgttca
      841 atgatccgag ggaaacttgg ggattggatc ttaagtattt tggaaaacaa atatgactta
      901 aatcacctgg acgcgatgaa attatatcaa tattccatac ggaacaatat agatatcttt
      961 atttatgtgg cgattgtcat tagtattctt attctatgtc gcgtcatgct ttcaaaattc
     1021 gcaaaatact ttgacgagat aaataccggc attgatgtac ttattcagaa cgaagataaa
     1081 caaattgagc tttctgcgga aatggatgtt atggaacaaa agctcaacac attaaaacgg
     1141 actctggaaa agcgagagca ggatgcaaag ctggccgaac aaagaaaaaa tgacgttgtt
     1201 atgtacttgg cgcacgatat taaaacgccc cttacatcca ttatcggtta tttgagcctg
     1261 cttgacgagg ctccagacat gccggtagat caaaaggcaa agtatgtgca tatcacgttg
     1321 gacaaagcgt atcgactcga acagctaatc gacgagtttt ttgagattac acggtataac
     1381 ctacaaacga taacgctaac aaaaacgcac atagacctat actatatgct ggtgcagatg
     1441 accgatgaat tttatcctca gctttccgca catggaaaac aggcggttat tcacgccccc
     1501 gaggatctga ccgtgtccgg cgaccctgat aaactcgcga gagtctttaa caacattttg
     1561 aaaaacgccg ctgcatacag tgaggataac agcatcattg acattaccgc gggcctctcc
     1621 ggggatgtgg tgtcaatcga attcaagaac actggaagca tcccaaaaga taagctagct
     1681 gccatatttg aaaagttcta taggctggac aatgctcgtt cttccgatac gggtggcgcg
     1741 ggacttggat tggcgattgc aaaagaaatt attgttcagc atggagggca gatttacgcg
     1801 gaaagcaatg ataactatac gacgtttagg gtagagcttc cagcgatgcc agacttggtt
     1861 gataaaagga ggtcctaaga gatgtatata attttttagg aaaatctcaa ggttatcttt
     1921 actttttctt aggaaattaa caatttaata ttaagaaacg gctcgttctt acacggtaga
     1981 cttaataccg taagaacgag ccgttttcgt tcttcagaga aagatttgac aagattacca
     2041 ttggcatccc cgttttattt ggtgcctttc acagaaaggg ttggtcttaa ttatgaataa
     2101 catcggcatt actgtttatg gatgtgagca ggatgaggca gatgcattcc atgctctttc
     2161 gcctcgcttt ggcgttatgg caacgataat taacgccaac gtgtcggaat ccaacgccaa
     2221 atccgcgcct ttcaatcaat gtatcagtgt gggacataaa tcagagattt ccgcctctat
     2281 tcttcttgcg ctgaagagag ccggtgtgaa atatatttct acccgaagca tcggctgcaa
     2341 tcatatagat acaactgctg ctaagagaat gggcatcact gtcgacaatg tggcgtactc
     2401 gccggatagc gttgccgatt atactatgat gctaattctt atggcagtac gcaacgtaaa
     2461 atcgattgtg cgctctgtgg aaaaacatga tttcaggttg gacagcgacc gtggcaaggt
     2521 actcagcgac atgacagttg gtgtggtggg aacgggccag ataggcaaag cggttattga
     2581 gcggctgcga ggatttggat gtaaagtgtt ggcttatagt cgcagccgaa gtatagaggt
     2641 aaactatgta ccgtttgatg agttgctgca aaatagcgat atcgttacgc ttcatgtgcc
     2701 gctcaatacg gatacgcact atattatcag ccacgaacaa atacagagaa tgaagcaagg
     2761 agcatttctt atcaatactg ggcgcggtcc acttgtagat acctatgagt tggttaaagc
     2821 attagaaaac gggaaactgg gcggtgccgc attggatgta ttggaaggag aggaagagtt
     2881 tttctactct gattgcaccc aaaaaccaat tgataatcaa tttttactta aacttcaaag
     2941 aatgcctaac gtgataatca caccgcatac ggcctattat accgagcaag cgttgcgtga
     3001 taccgttgaa aaaaccatta aaaactgttt ggattttgaa aggagacagg agcatgaata
     3061 gaataaaagt tgcaatactg tttgggggtt gctcagagga gcatgacgta tcggtaaaat
     3121 ctgcaataga gatagccgct aacattaata aagaaaaata cgagccgtta tacattggaa
     3181 ttacgaaatc tggtgtatgg aaaatgtgcg aaaaaccttg cgcggaatgg gaaaacgaca
     3241 attgctattc agctgtactc tcgccggata aaaaaatgca cggattactt gttaaaaaga
     3301 accatgaata tgaaatcaac catgttgatg tagcattttc agctttgcat ggcaagtcag
     3361 gtgaagatgg atccatacaa ggtctgtttg aattgtccgg tatccctttt gtaggctgcg
     3421 atattcaaag ctcagcaatt tgtatggaca aatcgttgac atacatcgtt gcgaaaaatg
     3481 ctgggatagc tactcccgcc ttttgggtta ttaataaaga tgataggccg gtggcagcta
     3541 cgtttaccta tcctgttttt gttaagccgg cgcgttcagg ctcatccttc ggtgtgaaaa
     3601 aagtcaatag cgcggacgaa ttggactacg caattgaatc ggcaagacaa tatgacagca
     3661 aaatcttaat tgagcaggct gtttcgggct gtgaggtcgg ttgtgcggta ttgggaaaca
     3721 gtgccgcgtt agttgttggc gaggtggacc aaatcaggct gcagtacgga atctttcgta
     3781 ttcatcagga agtcgagccg gaaaaaggct ctgaaaacgc agttataacc gttcccgcag
     3841 acctttcagc agaggagcga ggacggatac aggaaacggc aaaaaaaata tataaagcgc
     3901 tcggctgtag aggtctagcc cgtgtggata tgtttttaca agataacggc cgcattgtac
     3961 tgaacgaagt caatactctg cccggtttca cgtcatacag tcgttatccc cgtatgatgg
     4021 ccgctgcagg tattgcactt cccgaactga ttgaccgctt gatcgtatta gcgttaaagg
     4081 ggtgataagc atggaaatag gatttacttt tttagatgaa atagtacacg gtgttcgttg
     4141 ggacgctaaa tatgccactt gggataattt caccggaaaa ccggttgacg gttatgaagt
     4201 aaatcgcatt gtagggacat acgagttggc tgaatcgctt ttgaaggcaa aagaactggc
     4261 tgctacccaa gggtacggat tgcttctatg ggacggttac cgtcctaagc gtgctgtaaa
     4321 ctgttttatg caatgggctg cacagccgga aaataacctg acaaaggaaa gttattatcc
     4381 caatattgac cgaactgaga tgatttcaaa aggatacgtg gcttcaaaat caagccatag
     4441 ccgcggcagt gccattgatc ttacgcttta tcgattagac acgggtgagc ttgtaccaat
     4501 ggggagccga tttgatttta tggatgaacg ctctcatcat gcggcaaatg gaatatcatg
     4561 caatgaagcg caaaatcgca gacgtttgcg ctccatcatg gaaaacagtg ggtttgaagc
     4621 atatagcctc gaatggtggc actatgtatt aagagacgaa ccatacccca atagctattt
     4681 tgatttcccc gttaaataaa cttttaaccg ttgcacggac aaacggttct gttgcaaagt
     4741 tttaaataaa gaataaaatc ccttacggta tctatgattt aagctgggat tcccaata
//
LOCUS       DARBDV010000139         4788 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000139 DARBDV010000000
VERSION     DARBDV010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.145"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            564..989
                     /locus_tag="SCM56_002741"
     CDS             564..989
                     /locus_tag="SCM56_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111469.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            1019..1183
                     /locus_tag="SCM56_002742"
     CDS             1019..1183
                     /locus_tag="SCM56_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111470.1"
                     /translation="MKLTKIAVILAVIAGIILYFVLPANQFFLYFGIPAILIVNGVRI
                     FEQRKKCSST"
     gene            2141..3118
                     /locus_tag="SCM56_002743"
     CDS             2141..3118
                     /locus_tag="SCM56_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG1111471.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     YFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYSEKDPTSLQEKIKEQKRKLELL
                     QQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKKGLLDNLQNLFKE"
     gene            3128..3625
                     /locus_tag="SCM56_002744"
     CDS             3128..3625
                     /locus_tag="SCM56_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1111472.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            4001..4282
                     /locus_tag="SCM56_002745"
     CDS             4001..4282
                     /locus_tag="SCM56_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111473.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            4284..4700
                     /locus_tag="SCM56_002746"
     CDS             4284..4700
                     /locus_tag="SCM56_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111474.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1758 a          816 c          785 g         1429 t
ORIGIN      
        1 aaaaaaaaaa gaaaaatccg cttctgcgaa ttcttctttt ttcattttgg ggcgctgccc
       61 taatccctaa gtcccggtta ttcaatagca tggaaactca aagaataaga cgttccctgg
      121 aataaaaaaa aggctgctcg ttcgagcagt ccatacatag gaaaatgaaa aaacgacgtc
      181 ccttactgag aaacgctatt aaacttttaa caaatcctct aaaaaataac aagtctttaa
      241 aaagtgaccg gtaaaaatgc acaaagaact aaaaaagaga aattgcatca aatcaagcat
      301 tagaagctag taactagcta gaccaattga aaatcacaaa gtgagcgcct gtatctattg
      361 ctacaggcgc tacttggcat agtatacatt atgtcaacaa gaatttaata taacatattt
      421 ttctagtttg ttataacaat gccactctaa caaaaatgta agcattatcc ttattaaaag
      481 ctccttgaca aggactatac cttttatttt atgattttta catagaaaac gttttctcta
      541 ttttttgttt agaggtgatt tttatgattt gcaagaacaa gaaaaaaatt attgctatta
      601 ttgctagttt ctctttgatt tttagtactg catgtactgt cttattaaca aataatgcta
      661 atgttgaagc tgcaagctct tcttacaaac ataatcatgc tggttataga tgttacaaaa
      721 tgagttatta tgtaagtcct tctaaatgca aacaattggc tcgccaaact aataaactta
      781 ataccgtctc agctattgca aactttattg gactatctgg attaacaccg gggattatat
      841 cattagtttt cggatcagca gttagtgcta ataatgtatt tgttacagcc gctagaagag
      901 gaaaaggcgt tcaattaaat tatattgccc attttagtaa taccactacc tataattata
      961 gttctaacca aaattatgtt ataaaataat taaaacttta aactaggagg aatgctttat
     1021 gaaattaaca aaaattgctg taattttagc tgttattgct ggcattattt tatattttgt
     1081 attgcctgct aatcaattct tcctttactt tggaatacct gcgattttaa tagtaaatgg
     1141 cgtaagaatc tttgaacaac gaaaaaaatg tagctctacc taatcgttac aaaacaacaa
     1201 tttcaccttc ggtgaaataa gcgaaacttt acaaaaagtg gctagcaaat gctagccttt
     1261 tttgtattgt agagcgtgaa ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1321 aagcggttat ttgcaccaaa tacctccgta tttggctagc aatccttcta aaaaatacac
     1381 ctatcgatca gagtcaaaac aaatcaattt tcgagtgagc gaatccgaat ttgcaaagct
     1441 gaagctttgc aaatgagtgt gccggcttcc gtaaaaatac atatggctat aatttgcgtt
     1501 ttaaggcgtt ttaaacgttc tggtatataa ttataccaaa acaactttaa aacgccttag
     1561 atctaatttt agaggtctta tagaacattt tagggttgtt agggcttgcc ctgaccgcct
     1621 gtaaggcgct ttgagaacaa gataagggtt ttatactaag actgtcttaa aacggcttaa
     1681 acaaacgctc agaacttttc aaaggagttc tttatgaact gtttttaagc aaaggagcct
     1741 ttaggcgact gcgcagtata atgtgagagc gtagcgatca cacattttta gtgaccgtag
     1801 ggagcgagtt tcccagttta gaaaaattcg cgaacttgtt tgaggaaaaa agcaggagtt
     1861 ttttaggcgg gaaattttgc ctcctctttt ataatctctt tttaaacctc tttttaaacc
     1921 tctttaccgt cggctgtatg cttactctcc caaggtgtgc agaatctttt gggggtcttt
     1981 ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt ctttttgtct gtttttgggg
     2041 gtctttttgt ctgtttttag gggtctttaa ttaggggctt gacccctatt agaaagctct
     2101 ttataatttt gttaagtagt taatcttgaa ggaggatttt atggcaaatg aaattgtgaa
     2161 ataccaccat gaattaaata cgattccttt acgcaaattt acgtcagtag aaatgaattt
     2221 attcttctct atagtatcta gaatgcgtga cgtaggcgat aaaaaagtac aatttacatt
     2281 cgaacaatta aaagatttaa gcaattataa agcaactgca aatgttcgat tcatagatga
     2341 tttagaaaca acgtatgaca aattaatgga tttacgtttt ggtagaagaa gtgctgacgg
     2401 tttgcaacgt gaacgatttg ttttatttaa tcaatttaaa atcgatggaa aagctgatat
     2461 tccttttgct gaaatacaag tccatgagaa agccttaccc ttacttaata atcttgaaga
     2521 atgggttcga tattctctcc aacaattcaa tgaattagaa agcagttatt ccaaaaccat
     2581 gtttagatta ttaaaacaat acagaaccaa aggaattgcc tatttttcaa aagaagactt
     2641 tcatgaatta cttgatatcc ctaaaagtta tagagaaagt gatataaata aaaaaattat
     2701 aaagcctatt cgccaagaac taaccgcaat cttcaaaggt ctgacaatca aaaagaaata
     2761 cggaaaagga agaggaaaac ctgtcattgg ctatcaattc acgtttaaac ctgaaataaa
     2821 aaaagcagac gactttaata aacacgcacc aacaaaagaa gaacaaaata aagctttaga
     2881 actagttaat caacaaattt actctgaaaa agaccccact tcattacaag aaaaaatcaa
     2941 agaacagaaa cgaaaattag aacttttaca acaattagaa gagctagaaa gaaaacaaaa
     3001 taatctgaat aaagaaaata aggagattaa cgaacaaata ccagataaca attccgatat
     3061 tcccccagaa ttaaaaaaag gcttactaga taatcttcaa aacttattta aagaataagg
     3121 ggtttttatg acagataaaa ctatcaaaga attagcagat attctaggtg tttcaaaaga
     3181 taaagtgaag tatcaactga ggaaattacc tagtaatttt acctacaaaa aaggaaatat
     3241 tatctacctg aaaaaagaag cagtaacaac aattacaaag gttttatcag gtaatgagcc
     3301 gggtaactta cctagtgaat tacctgattc attacctacc gattacctac tcaaggagat
     3361 tgaaactaag aataatcata ttgaagaaaa agataggcaa attgaaaaac ttcaactagc
     3421 tttagaaaca caacaaaaat tactcgacca gcaacaacaa ctcaccctgc aagccaacca
     3481 acaaatcgag aaactgcagg aacaactaca gttgacttat acggaagaat cttcggaaaa
     3541 taaatcaacg actctttctg aagaaacaga atcgatagaa aaacaatcaa agacggagaa
     3601 aaaatggtgg caattctggc aataaaacca gcaatttaaa ttaataaaaa ttgctggttt
     3661 tattcttctt tttgcttttg cgagcagagc aacatgggcc catgttgcga tattttggtg
     3721 gcgccccaat ccccgttttt tcgttgaatc ttactcggac tgcactcgtt ttacagctga
     3781 tttttctgaa ttttgaaaga gcataaggaa acgtggtatc actttcttac gaaaggtgct
     3841 agataagctg tgcctatcac gtttctcaga aaaaaatttg ctatcacttt tttgccattg
     3901 tggctaggct atgtatagtg ttttggcgaa aaatagctat acaaatacta tatttgtatg
     3961 atatacataa attatatact gatacgaaag gaaaatttcc atggacattt tagttcgaaa
     4021 tatcaatccc aaaattatta caagaataga taaacgttgt caggaactaa caaccaaaac
     4081 gggcaaaaaa tggagtcgaa atcaatacct aaaaatttta atcgaaaacg attttgacca
     4141 cgaacttatg aattacaaaa aagatcaatt cgatttatta ttcgaaaaat ttattgccat
     4201 tcaagcagaa aatacgaaca tactaaatga ctatgttcac acaaacaacc aactcattga
     4261 aacacttatc ggaggaatat aacatgagaa aaacttctgt tcaaaaacac gtaacccttt
     4321 atcccagtca cgttgacgtt ttagattcca tcatcgaaaa aaatccagac gtcaacaatt
     4381 attcagaagc cattcgcttt ttatgtatga gttatgaacc tgctaccaat acaaccacaa
     4441 ccagtaacca agcaaaactc aatgccatgg gaaaagaaat ttctatcatg acagaaatca
     4501 tggcagaatt tgctgattta tatctaaaag atactggtgt tcttatcggt acaaattcta
     4561 aagtatatca agaagtgaaa gaaaaaattg aaaatcaact caatccttcc cgacgaaatc
     4621 aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac ttctggaaat tttgacccaa
     4681 caaactttct aaaaagataa aaaaagaaac cgaaaaataa aaaaattcct cgatttcttt
     4741 ttagggatcg gtcttccagc aagatttgca agttggtacc aacttgcg
//
LOCUS       DARBDV010000140         4746 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000140 DARBDV010000000
VERSION     DARBDV010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.146"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(182..1144)
                     /locus_tag="SCM56_002747"
     CDS             complement(182..1144)
                     /locus_tag="SCM56_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1111475.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1146..2585)
                     /locus_tag="SCM56_002748"
     CDS             complement(1146..2585)
                     /locus_tag="SCM56_002748"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HEG1111476.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2770..4716
                     /locus_tag="SCM56_002749"
     CDS             2770..4716
                     /locus_tag="SCM56_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEG1111477.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1509 a          810 c          785 g         1642 t
ORIGIN      
        1 acagttgtgt aaaaatatca ttttagagca tgaaaaaagg agtggatttc tccactccaa
       61 aaaagtctaa cttttggggg tcactacaag ttgacaactc ctttttgttt acaaatcaat
      121 tggatatcta caaattgtat tttctaacga attttataga cattaaaaaa ttaatttaac
      181 gttaaatact tcctccagaa agtattgaga ttggtaaaat ataatcctta tttgtctttt
      241 cgcctttaat ttttttcaga agaatatcaa caataagact tgcaatttca tcaattggtt
      301 gtcttattgt tgtcaattgt gggaaaaatt tctcaataaa agaggtacca tcgtatccaa
      361 taatttttaa atcttctggg atattaagtt taagttgatg agcaattttc atagtgagaa
      421 tggctgttaa atcatcagaa acaaaaatac catctggttt gtgatttata atagttgact
      481 ttatttccat ttcacgacgg atagtagaca gattattagg aattttgaaa atttttccat
      541 ttggattttg aaaagaaaaa cctagtgcac gtaattctgt gggagaatcc gtgttatcat
      601 ttcctgtaat cataatggta ttttgacaac catttttttg taaagtctta gctgcaagtt
      661 ttcccccttc aaagttatct gaagaaacaa tggaaacatt aggagctaaa tttctatcaa
      721 aagcaacaat aggtgctctc acacgctcat aatcagcaat tccaagatta tggcttgatg
      781 agattatgcc atcaacttgg ttggcttcaa gcatttcaat gtattctctc tctttagcag
      841 gatcattttg actattgcaa ataatcgttt tatatccatg tttaaataac tctatttcga
      901 gatactcaat gagttcagag taaaagatat tactaatatt aggaaaaata agaccaataa
      961 gctgaggtga ctttccttga agtccacgtg ctaaattatt tggtttataa ccaagagttt
     1021 tcatagcttc atgaactttt tgttttgttt tttctgataa atatccctta ttattaatga
     1081 cgcgtgaaac ggttgttggg ctaactccag ctaattcagc gacatcagtt aattttgcta
     1141 ccattttaat attttaattc aaagtatttt ccagtaggtt cgcctgattt gatttgaata
     1201 ccattttggt gattttcagg gaatacacga ctagtaaaca ctttttctcc tttattaata
     1261 aaaatctcaa tgattgagtt atctacaaaa atgttagcag ttgtttcttt agtatcaatt
     1321 gagcattcac gaatcgtacc aaagtcagtg gcatattgaa tgcctgcttt gctgcgatct
     1381 aaaataattt ttccttcttt ggtatctaca atcaagctaa gtccatttcc tttactatca
     1441 gagaaaagaa gtaactccga tttctgattt ggaggaaaag ttagttcaag ctcataagta
     1501 ttatttgttt ctgcttttgc tgtaaattgt tcagaactag cacgtaatga tgtaatagct
     1561 tctactggat attggtaaag tttaccatct tttattgaaa gttcttttac aagactcatt
     1621 gctccctgat aatcatactt atcagttgga taatcgatat caggtagtcc aatccaactg
     1681 acaatcagag cacgaccatc aggagtattg aatccttgag tcgcatacgc ttcaaatcca
     1741 taatcaagat tttgaatttc tgaagcgccc acaagtttta ctttttctat gtcaagcgat
     1801 tgacagatct taaaagtatt cggatagata ttcccataat ttagctcaga tttatccaac
     1861 ccttgaggac aataaatgag aacaggtttt ttgtctacaa atactagatt tggacactca
     1921 atcatgtatt ctgaaccagt gccgccaaaa tcaaggttgc caacttcctc ccatgtttcc
     1981 acgttattgt catccgcttt gtaaagtttg ataaagcctg atttttctaa attttgtgct
     2041 ccaacaatcg catacaattg tccttcataa ttaaacattt gtggatcacg aaaatgatca
     2101 gttacatcag caggtttatg aataagaaca ttctcacctt tcgtaatgtt gtagtcttta
     2161 tccatccatg ctccgatttg aagtgtatca cgttcccaat tttcatttcg aacgtttcct
     2221 gtatagagta agaatagatt cccctctatt tcatatgcag aaccggaata agcgccgtgg
     2281 ctatctcgtt ttgtgtctgg aagaagttct gctcctgtta catgaaaatg aactaaatca
     2341 tctgattcat tgtgtaccca ttctttcaac ccatgagcag ctccaaatgg ccaattttga
     2401 taaaaaagcg tatattttcc gttaaaaaaa gaaaaaccat taggatcgtt taacaatcct
     2461 gttttaggtt cgatatgata gctagcacgc caaggtgatc gttttacatt atccttaatt
     2521 ttatccatct catctttagt ccaatcagaa taagcacgat atcgtatttc tcgaggtaag
     2581 ttcatatttt actccttcaa tttatctttt tacaactagt atggtaaacg ttttccgttg
     2641 aaaaatcaat catttcattt aaaaaaataa actatatgac aaacgtttga catattttta
     2701 agatatgcta aaataaattt gcatttcagt gaaccgtttt cacgtaaaaa taaaaaagga
     2761 gtgattcgaa tgaattacag taaagtagct tccgacgtaa tacaagcggt cggaaaagat
     2821 aaccttattg cagctgcaca ctgcgctaca cgtcttcgtc tcgtactaaa agatgacacg
     2881 aaagtgaatc aaaaagcttt agacgaaaat ccagatgtga aaagcacatt taaaattaat
     2941 ggacaatatc aagtgattat cggtgcaggt gatgttaatt tcgtttatga cgaacttatc
     3001 aaaaaaactg gactttcgga aatttcaaca gatgatttaa aacaaattgc tagtaaagat
     3061 aaaaaattca accccattat ggcattaatt aaattattat cagatatttt tgttccaatt
     3121 atacctgccc ttgttgcagg tggtttatta atggcattac aaaacttttt aacagctgct
     3181 ggactttttg gaccaaaatc aattgaagaa atgtatcctg cgattgaagg tatttcctca
     3241 atgatccaac tgatgtccgc tgcaccattc atgttcttac caattttagt aggtatttca
     3301 gctgctaaac gttttggagc aaaccaattt cttggtgctg caattggtat gatcatgaca
     3361 acaccagatc ttggtggctc aactgaatat tggaatattt ttggttatca tgtttcacaa
     3421 acaaactatg cttatcaagt tattccagtt ttagcagctg tttggatatt atctatatta
     3481 gaaaaattct ttcataaaaa attgccatca tctattgact ttacattcac gccattatta
     3541 tcagtaatga ttactggatt tttgactttc acagttattg gccctgtaat gttattgttg
     3601 tctaatggaa ttacagacat tattgtttgg ctatataaca ctactggatt tattggaatg
     3661 ggtatttttg gaggaacata ttcacttata gtcatgactg gtttacatca atcatttcca
     3721 gctattgaaa ctcaattact ttcagcgtgg acaaatggta tcggacatgg agactttatc
     3781 tttgtagttg catctatggc aaacgttgct caaggtgctg caacatttgc catttggttc
     3841 ttaactaaaa attctaagac aaaaagtctt gcttcctctg cgggtgtctc agcacttctt
     3901 ggaattacag agcctgcctt gttcggagtt aacttaaaat accgctttcc attcttttgt
     3961 gctttaattg gttcaggaat tgctacagca gtagcaggat tacttaaagt agttgctgtt
     4021 tcacttggtt cagcaggttt cttgggattt ctttcaatta atgcaacatc tattccattt
     4081 tacgtattgt gtgaattgat tagctttgta gttgcttttg caattactta cttctacggt
     4141 aaaacaaaag ctgctggtat ctttaaagca gaagaaattg ctgaacaaac accagttaat
     4201 tcttcaaaag tagcaacaac agatccaata tctactgatg ttgaatcaac acaaacaaaa
     4261 gtttcatcag aaacaattgt tagtcccatt tctggtaaag tgattagtct ttcttcagta
     4321 aatgatccag tcttttcatc agaagctatg ggaaaaggga ttgccattaa accaaatgga
     4381 aacacggtcc attctccagt agatggtact attcaactca cgtttgaaac tggacatgct
     4441 tatggtatta aatcaaataa tggtgctgaa gttctaattc atattggtat tgatactgta
     4501 tcaatgggag gaaaaggatt tacacaaaaa gttactacta atcaaaaagt tagaaaaggc
     4561 gatattcttg gagcatttga ccgttcaatg attgagaaag aaggtcttga tgatacaaca
     4621 atgattattg tgaccaatac ttcggattat tcagaagtaa atatagtagc tgaaaataca
     4681 gtcactgagg gtactgattt acttgaaatc aaataaatat atttccctga gtaaagatgg
     4741 aatttt
//
LOCUS       DARBDV010000141         4646 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000141 DARBDV010000000
VERSION     DARBDV010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.147"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(133..717)
                     /locus_tag="SCM56_002750"
     CDS             complement(133..717)
                     /locus_tag="SCM56_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1111478.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            complement(928..1131)
                     /locus_tag="SCM56_002751"
     CDS             complement(928..1131)
                     /locus_tag="SCM56_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111479.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            complement(1141..1563)
                     /locus_tag="SCM56_002752"
     CDS             complement(1141..1563)
                     /locus_tag="SCM56_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111480.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRDGIAIGNPTNDLTVTENYLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            1801..2415
                     /locus_tag="SCM56_002753"
     CDS             1801..2415
                     /locus_tag="SCM56_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1111481.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(2580..3404)
                     /locus_tag="SCM56_002754"
     CDS             complement(2580..3404)
                     /locus_tag="SCM56_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111482.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
     gene            complement(3428..3583)
                     /locus_tag="SCM56_002755"
     CDS             complement(3428..3583)
                     /locus_tag="SCM56_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111483.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFILKKN"
     gene            complement(4042..4299)
                     /locus_tag="SCM56_002756"
     CDS             complement(4042..4299)
                     /locus_tag="SCM56_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEG1111484.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(4292..4561)
                     /locus_tag="SCM56_002757"
     CDS             complement(4292..4561)
                     /locus_tag="SCM56_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEG1111485.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
BASE COUNT         1533 a          816 c          626 g         1671 t
ORIGIN      
        1 taatattaac aaatgaataa aatccttaca acatttgaga ataagttatc agtagacact
       61 tacaaaaaat tctcatacta ttgtcacttt tctagataaa tggcaaaaat tttcagcatt
      121 aaatgacaaa aactaatgag aatttgtctt tccatttttc tcctccattt ttgccaaata
      181 acgataaatg gtggttcttg agacgttcca ccgttccgca atagtcttaa taggtgtacc
      241 gctatccact aaagtcttca gtaattccaa atcttgttca gacaattttt caggacgacc
      301 accaaaccgt cctcttgcac gagcagctgc tcgtcctgct gcggatcgtt ccaggatcaa
      361 attacgctca aattctgcaa atgccgcaaa caaatgaaac atgagctgtc ctgtagaact
      421 tgatttgtcc atcgtaatat tttcttgaat actatgaaaa ctcacaccac gctcattcag
      481 tcgattgacg attgaaatca aatcttccat atttctacct aaacgatcta agcgccaaac
      541 gacaagtgta tcgccagagc gaacaaaatc aatggccatt tctaatcctg gtcgattact
      601 ttttgcccca ctcatatgat cagtaaaaat tttttcacaa ccaaatttat ttaggctgtc
      661 ttcttgtaaa tccaaatttt gtaaaccagt agatacccgg gcgtatccta ttttcaaaaa
      721 aatcactcct tcattaattt tattgtaaca taactttatc catatagttt aactgaacat
      781 agatattgtt atgggtttat gaacatgaaa tgaattgttt ttttgaaaaa taaagcaaaa
      841 aaagaaagtg tccagaaatg ggtgatttta tgaacacttt caattagaaa aaaagaatgt
      901 cgactagaca ctcttttttt aaaatcatta tttttctttt tgatatgttt tttggtctct
      961 tttttttctt ttttcttcag aatcttctag attagaaatt gatttttgaa ttttttgtgt
     1021 ctcctgtaaa gttcgctgaa cttgtaaagc tgattcttct ttcggtaaat taattttact
     1081 tagaatatcg ctatcttgat aaaaatttgg tgtgttcaca aattctggca taaaaaatcc
     1141 ctactttcta gttaaggttc ctgaataagt cgcaatccaa ttgtttccag ctgatttata
     1201 actttgtaaa tacaattttc catagtacca ataaccattt cttttttctt tccaattccg
     1261 tgattgagga ggtctagaag gccaaccaac tttatcaaaa atcatcgttc ctgtcactcg
     1321 tatcgtttta gaagctgcta gagcaagtaa tggagaaact attaaataat tttcagtaac
     1381 agttaagtcg ttagttgggt taccaatggc tattccgtcc ctatctaaag gagttaattc
     1441 ttggttatta tctactacag tagttggttc atttgtacta tcagcaaatg taggaattgt
     1501 agttccagtt acactaattc caatagttaa aatacctaaa gataaaataa acttattttt
     1561 cattgtctat cacctcgaaa tcaaaactaa gaaaacgttt tcttaataaa tcttaacaga
     1621 taaatcaagt tatatactag aaaattcata aaatatattt ttaatgtcat aatcgtttaa
     1681 attttcttga ttgacattta tttacaaaaa atattgccat ttaaaaagaa aagtgacata
     1741 tggcttcctc gtgtatgatt agggtgtatt ttaaatagtt gcagaaagta ggtaaaagtt
     1801 ttgagagttg catacatacg tgtctcatcg atcgatcaaa atgaacaacg ccagattgaa
     1861 gaaatgaaaa aattcggtgc agagagaatt ttcatcgaga aacaatctgg cgcaacgatt
     1921 actcatcgac ctgttttcca agaagcatta gattacgtcc gagatcaaga tatttttatt
     1981 gtggaagcta ttgatcgact tggaagaaat tatgacgaga tcatcgcttc agtcaattac
     2041 ttgaagaaga aaaatgtgaa gctgatcatt actagtttac cgatcatggc tgaagcaatt
     2101 ggcaatcctc tcctggataa attcattaag gatctgatca tccaaatttt ggccatgatc
     2161 gctgaacaag aacggactga atcgaaacgt cgccaggcac aaggaattaa gatcgcaaaa
     2221 gcaaacggcg tctacaaagg gcggcccaaa ttatacagtg cagacgcaaa agatccccaa
     2281 cgtcgccttg tttacaaaag catcgttgaa gatcttaaaa atggtgttgc gattgctaag
     2341 attgctaaag actacaatgt tacgagacaa accgtgtata gaataaaaaa agacagcatg
     2401 gtcaatgaca aatagttgat catgctgctt ttattatctt ctcatcttta atttcctaca
     2461 tataattact atctataaaa ttaagtctta ctcagaacag ataaagcata aaattccaac
     2521 aaaccatttt ttacttaact aaataaagag tctgttaata taatcttcac ttatttacgt
     2581 taatccccta ctgtatttgt tggttcctta tctatatttt ctacaaaatc cttattatct
     2641 tgtgatttag cttcgtataa ttctttgtat gtcgacacat tggttatact aattcctata
     2701 gctagttctt gagactctac ttttctctct agctcctttg acaaagtgat ttgagattta
     2761 tttggttccg ttggagtcaa ttctatagat tcttcataaa aatctctata cataaagcta
     2821 tcattaattt tataaaaaaa attaaatgtt tttagcactg ccatataaca ttcaaaacat
     2881 tcttctatag ttggtttact tccttgagga gaatgaacca atttattacg tttttctatg
     2941 aaatttttaa tttcctttct tatttctttc ggaaactcaa tattaaagta ccctttaaac
     3001 atgctcccta ataatttgtc tacaggggga gattgtaaat tctcaaatat tagagttaaa
     3061 tcaggtttct cttgttgata aaattccttt acccctattt cacaggctgt aattatcgaa
     3121 ataaacctat ctctagtatt tgatatattt ttacttcttt taaggagtat aagagaaaag
     3181 gggatacttt cgtgtccatg catgtaatta actaattgtt gagcttcaga agttatatct
     3241 ctaattatat agttaacgga attaggaagt gtctttaact ctaatgaaat ttctatccaa
     3301 ttactatctt ctaatctgta tatgctcgaa gtataatttg attcgtcagc aagcatcgct
     3361 acatcccaaa cactgatcat aaaatctttt aaacgttcaa tcatttcagc aatattgcta
     3421 aaatcagcta atttttcttt agaataaaaa aaacatttat atcagaatca ataaattcta
     3481 ttttttttat tactatatcg tcatcattat cttgaccatt tatgtttatt gttccctgag
     3541 tggggagtac ttctttcaaa ttcaagttaa atttatattc cattacttct cctccaatat
     3601 ataaaaatta cttaactgta tccaaattta atatgtaaat aaatcattaa tttttagcta
     3661 aattctatga gaagtttatt tttgttgttt ttgctaatta actaaaacta gtacaaaaaa
     3721 tttaaaatag attattaaac ctattgctta ttctattcag tcatatatat aagcgtcatc
     3781 ctttagtaac taacatccca cactacaata gtattataac atatctattc aacttcaaat
     3841 tgttgaaatt taagccattt atatttactt ttcctcaact ttagaaatga ttttactaac
     3901 cttatatccg taatacgcgt aacaaacaca ttcattaata acaatcaaca ctaagctata
     3961 ttagcttttt aaatgactag ggtaaataaa ataaggatca aaaactttta agtttctgac
     4021 cctttcctta cttccgattg gttaatagtg atcttttgca gaataaataa atatcgtttc
     4081 attttcaact ctatatatca gtctatgttc atctgtaatt cttctggacc attttccaga
     4141 taaatcatgc tttaatggct caggttttcc taatccagca aagggggaac gatcgatatc
     4201 ttttattaac ttgttaatct tttttatatt gcttttgttt ccttgctcat gccaataaag
     4261 ataatcatcc caagcatcat cagaccaagc cttaatcatc agattcaacc tcgattaagt
     4321 catgtgtttt aaatgcacct ttggagaatt gttcatctcc tcgacgaatt ttttccatga
     4381 cgtaattatt agaaagtgtt ctcaacgttt cttgcataga atcataatct ctttttgata
     4441 atacaacaac tgtatcttct acatctttac ttgttacaat aagtgtttca gcatcctcat
     4501 taacttgttt catataacta cgtaaatttt ggcggaaatt tgaataagct actgcttcca
     4561 ttcctttcac cccttattta tttctataaa acaattgtac attatattgt acaattaagc
     4621 aaatgattaa tattctaact tcttat
//
LOCUS       DARBDV010000142         4473 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000142 DARBDV010000000
VERSION     DARBDV010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.148"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1389
                     /gene="gadC"
                     /locus_tag="SCM56_002758"
     CDS             <1..1389
                     /gene="gadC"
                     /locus_tag="SCM56_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HEG1111486.1"
                     /translation="GGILWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAI
                     FFQWFQITVGFITMIYFILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTN
                     RTAKIAKAGFVIGIVIPSIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFV
                     SFILAYMGVEASASHINELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLS
                     LSAGVIQTFETLILHFNSHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGL
                     LPVFFRKTNKQDVPVPLIMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGY
                     LLFFLGYFVLVFKKKDLKRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAAS
                     EHRTYQIILLISFVVTVLLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEH
                     HVTKNDEHILKH"
     gene            1402..2802
                     /locus_tag="SCM56_002759"
     CDS             1402..2802
                     /locus_tag="SCM56_002759"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HEG1111487.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(2967..4407)
                     /locus_tag="SCM56_002760"
                     /pseudo
     CDS             complement(2967..4407)
                     /locus_tag="SCM56_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT         1400 a          741 c          851 g         1481 t
ORIGIN      
        1 ggaggtattt tatggtttct accagttgct ttatgtgctg cagaaatggc aacagttgaa
       61 ggatggaaag atggtgggat tttcagttgg gttagtcaaa ctttaggaga acgttttggt
      121 tttgcagcta tttttttcca atggtttcaa attacagtag gttttattac aatgatctac
      181 ttcatactag gagcgctgtc gtatgtaatt aattttccag agcttaatac agatccattt
      241 atcaagttta ttggtgtatt agtcattttc tgggtactga ctttttcaca gttgggcggg
      301 accaatcgta ccgctaaaat tgctaaagca ggatttgtca ttggaattgt tattccttcg
      361 atcattctct ttggtttagc agcagcctac tttatcggcg gtaatccgat tcaaatggct
      421 attaatgcga aagcttttat tcctgacttt tctcagcttt caactttagt agtatttgta
      481 tcttttatat tggcttatat gggcgtagaa gcttcagcat cacatattaa tgagctggaa
      541 aatccccaaa gaaactaccc attggcaatg tttttactag tgattttggc catcgcctta
      601 gatgcaatcg gcggtttctc agtagcagcg gttattccac aacatgactt gtctctcagt
      661 gctggtgtaa tccaaacatt tgaaacgctt attttacatt ttaacagtca tttacattgg
      721 ttggtaaaag tgattgctct catgattgcc tgtggcgtta tgggtgaagt tagttcttgg
      781 gtagttggtc catcacgagg gatgtttgca gctgctcagc gaggtctgtt accagttttc
      841 ttccggaaaa ctaataaaca agacgttcca gttccgttga ttatgataca aggaattatc
      901 gttactattt gggcagcagt cttaactttt ggtggaggag gcaacaattt atcattctta
      961 gtagcaatct ccttaactgt tgtcatttat ctcgtaggat atttactatt tttcctaggt
     1021 tactttgtat tagtttttaa gaaaaaagac ttaaaacgaa cttacaatgt accaggaaaa
     1081 actgttggca aaacgattat cggtggaatt ggttttattt tatcagtgtt cgcattgctt
     1141 atctcttttg taccaccagc ttcaattgca gctagtgagc accgaacata ccaaatcata
     1201 ctacttatta gctttgtagt gactgttctt ttacctttta tcatttatga agtacatgat
     1261 aaaagtgctc acacaactat tgaggaacct actcacgtga aagctgaaga cgttaatccc
     1321 gctgtttatc cagctgctag aggagagcac cacgttacaa aaaatgatga acatatttta
     1381 aaacattagg aggaattcat tatgttatac ggaaaagata atcaagaaga aaaaaactat
     1441 ttggaaccaa tttttggctc tgcaagtgag gatgttgact tgcctaaata taagttaaac
     1501 aaagaatcca ttgaaccacg aattgcttat caattagtac aagacgagat gttggatgaa
     1561 ggaaatgcgc gattaaactt agctactttt tgtcaaacgt atatggaacc tgaagcagtg
     1621 aaattgatga cccaaacgtt agaaaaaaat gcaattgata aatcagaata cccacgaaca
     1681 acggaaattg aaaaccgctg tgtaaatatg attgctgatt tatggcatgc tccaaataat
     1741 gaaaaattca tgggaacttc aacgatcggc tcttcagaag cctgcatgct gggtggtatg
     1801 gccatgaaat ttgcttggcg taaacgtgct gaaaaattag gtcttgatat tcaagcaaaa
     1861 aaacctaacc tggtgatctc ttctggttac caagtttgtt gggaaaaatt ctgtgtatat
     1921 tgggatgtgg aactgagaga agtcccaatg gatgaaaaac atatgtcaat taatctagat
     1981 actgtcatgg attatgtgga tgagtacaca attggtattg taggtattat gggtattact
     2041 tacactggtc gttatgatga tatcaagggt ctgaatgatt tagttgaagc tcacaataaa
     2101 caaactgact ataaagtata cattcatgtt gacgctgcat cgggtggctt ttatgcacca
     2161 tttactgaac ctgatctagt ttgggatttt caattgaaaa atgttatctc aattaattct
     2221 tcaggtcaca aatatggttt ggtatatcca ggtgtgggtt gggtcttatg gcgtgaccaa
     2281 caatacttac cagaagaatt agtatttaaa gttagttact taggtggaga aatgccaact
     2341 atggctatca acttctctca tagtgcagca caactaattg ggcaatacta caactttgta
     2401 cgctatggct ttgatggtta tcgtgatatt caccaaagaa ctcatgatgt tgctgtttat
     2461 ttagccaaag agatcgaaaa aactggtatt tttgaaatca ttaatgatgg atcagaattg
     2521 ccagttgtgt gctataagct gaaagaagat cccaatcgcg aatggacact atatgattta
     2581 tctgatcgtc tgttaatgaa gggatggcaa gtcccagcct acccattacc taaagacttg
     2641 gatcaattaa ttattcaacg cttagttgtt cgagcagact ttggaatgaa catggctggt
     2701 gattatgtac aagatatgaa ccaagcaatt gaagagttga ataaagctca tattgtttat
     2761 cataaaaaac aggatgtgaa aacgtacggc tttactcact aaaattcttc agagaaatat
     2821 aaaagaagtt aattaactaa taaaaggagt tgtgattacc acacagctcc ttaaatctat
     2881 ttgaataaat atatgatatt catttgttac actctcaatc ttagctgttg ttctattcat
     2941 cattactctt attgtaagta gttcagtcaa atttgcttgt tcaattgatc gataacagta
     3001 tgaattgttt gaaaagtaat cggatatata attgttttct cactacctaa gccttcttgg
     3061 tataaagaac tatcgcaaag tataatccgc tgagtacaaa agaaagtgtc gtctactatt
     3121 tctctaagtt gtttacaaat gatattacta atagaaaagt gttctcccaa aatgtcttct
     3181 aactgttgtc gatagtataa atacctaaag tctgacggag ctactatgaa cacttcaatt
     3241 ggagatttcc gtttatgctc atttaaaaga cttaaactaa tggttagttt acgaatagaa
     3301 ttctcattcc aacgcagaaa taaattgttt tgttttcgcc attgttcaag aatttcatga
     3361 acttctaagt acaaagcgcg ttgctcttca tttaacaagt aagtcttatc aggttgaaat
     3421 atctgaatat cagcccaaag tgagcggata aacgggagaa gtgctttatc aaaaagtatc
     3481 tgattttctt cagatatatt cagagataag ataagttttt gactgagctc aatcaccaaa
     3541 gggttatctg ctacatggtt ttgataaaca acgttcaagt ctttttgtaa caattcaaag
     3601 ttatcgcata gataatttct agagttgaat agaaagtaga tataaaggca ttcatcttcg
     3661 tcatgacata aagagaaaga aagttgctct aatcctttct gtattagctg aaacaaggga
     3721 gtatgtcggg caactctttt ttcttcctga ctaaaatata ttgggttagt cttagatctc
     3781 tgcaaaccga tagcgatacc taataaaata aattgttttt caactgtagg gtaacttctt
     3841 gaaaaaaaat gtttttctac ataatcaatc aattgttgac cgctttgaat aattgattta
     3901 tttagctgga tcaactctgt ccgatttgtc tcatagatta tcgttagtaa caaacttcga
     3961 taatcctttt caggaatttc aaaatggcca tcagcattta agtactgcat ttgtttaaaa
     4021 aaattaatca actcatttcg taagttgtaa actttgctta acgaaatgaa ggtagtatca
     4081 gctatattac tccaattgtg ttcccctgat aaataatgtc ctagtacatt gaaaaagtca
     4141 gagcgctgat agatgagttg agtgagtgcc aacctcgaaa acgaagagtc gaatgcgact
     4201 gtaaagctac ttctagattt ctccactgaa ataatttgct gatggagtat gtcggtaatg
     4261 cattccaagt cgttagtgac agacagtgag tgggcaaacg ttcaattgat ctgctatttc
     4321 gcgaatctga atggtccctt tttcgtgtaa ttgctcacaa atgtaaactt gacgaaaaat
     4381 acttttctcc agataggttt ccaacatagt actcctccta gtcattttat acataactat
     4441 tataactcta ccaatcggaa tagtcctgta ata
//
LOCUS       DARBDV010000143         4471 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000143 DARBDV010000000
VERSION     DARBDV010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.149"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            319..1050
                     /locus_tag="SCM56_002761"
     CDS             319..1050
                     /locus_tag="SCM56_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEG1111488.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1195..3222
                     /locus_tag="SCM56_002762"
     CDS             1195..3222
                     /locus_tag="SCM56_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEG1111489.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3330..4427
                     /locus_tag="SCM56_002763"
     CDS             3330..4427
                     /locus_tag="SCM56_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEG1111490.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1570 a          717 c          923 g         1261 t
ORIGIN      
        1 aatataatat acatttttta tttaattata attaatattg attgaagtct aggcagtcaa
       61 gtcacatttt gatagatagg agggggactt acgaattatt ttaaaggcta tgacagctgt
      121 atttctagat tgtgtttgca tcaagttgca ataaaaacta gaaatcaaat cgggctgata
      181 atcatttata actaatcgat acataggacc aaatataggt ctatgttctt ttatttcata
      241 agtatcgtaa aacagaattt tgctatggat taatccaatc gactgatatg tttagtaaat
      301 aaagaaagag gaatgtcaat gatgaaaaaa gaaatcgtat taacagcagc aatgatgtta
      361 ttcagcatgg tagcaagtac gacttttgtt tctgcaactg aagtataccc aaaggaatat
      421 aacacagaag gaacgattac atttgaagca ggagatgagg gtgtaactcc tcctgttgat
      481 ccagagaatc ctgatcccaa taaacctgtc gatccatcag atcctccaag ccctggaaca
      541 ggaggggcat tatcgattga ttatggatca aaattcaaat ttgggactca aaaaatttct
      601 actgcggata aaacgtatta tgcagctgct gatgtgatga acgatgggtc tagaaaaccg
      661 acatatgtcc aagtgacaga taggagaagt accttatctg ggtggaaatt aagtgtctct
      721 caaccagaac agtttaaaac tgccagtggg gatgaactag ttggagcaca gctgaaattt
      781 acgaaagggc aggctgtttc tttggttgat ccaacatata cgccgcaaac ggtgaattct
      841 gaattaacgt tgacaccggg aggaaacaat acgttagcaa ttaatgctaa atcaggaaca
      901 ggtgtgggga cttgggtcta tcgatttggt gcaaatgaaa atgaaaatca agatgcggta
      961 cagctttcgg tacccggtaa atctgtcaag ttagcgcaac aatattccac taaattagtt
     1021 tggacattag aagatacacc taacaactag tttactgaaa agaactgtta aaaataaata
     1081 cgtagtcata ttgtcttgcg aggtaatcga gcaaaaattg tcaaaggcta ggctaaattt
     1141 gtttggatac gaatagttca tctaggaatt taacgatcag gaggaaaaga tatattgaaa
     1201 aagaggagga tcgtgctcat tttaagcagt ctcttagtga ctaatatctt tccgatcata
     1261 gggtactctt tggaattgaa tcagttggag catggaaata gaagagacga agaaagcaaa
     1321 ctcacttcga cattcgacag tttaatagaa tccaaccaga cacaagaaag cagtgaggat
     1381 ttaagtattt cgagtagtgg agagaccact gacaacgtag aagctgaggg ttcggacaaa
     1441 caaggactag aagatgcacc aatttctgcg gttgagctgc ctgacgatcc caatattatt
     1501 tctatcgata aaatttttca aacacctatc ggtactagca cgtccatatt ggaaaatggg
     1561 aagttactgc agttgaaccc agcagaaagg tctcaaagag gagctatttg gtcaaataag
     1621 aagattagtt tactctcgga ttttacattt aaatcttatt tatacttagg aaatcaacga
     1681 gatagagcgg gagatggcat gacctttaca ttgactggcg atcctagaat ggctagtaat
     1741 cccagtcaag ttattggatc gccaggtatg ggaattggct catattctac tcgttctggc
     1801 caaccttaca ttgcgaatgc gctctcgatt gagtttgata cgtataaaaa tagtggttct
     1861 tctgatcgaa tggatcgaga gataactgct gataatagag ggcatggaca tgtcgctttt
     1921 gtcacgccta aaccaaataa taataattat tctggagagc atactggcgt gacggtagct
     1981 cctacatttc tttctaatgg cacatggcgc ttgttaataa ttaaatggga cgcggagcaa
     2041 agaagtctta cttatgattt ggaaggtgtc ggtcactctt ctcggattat acctgatctc
     2101 attaggcagt ttggttctac agaagtaaat tgggggttta cttcttcaac aggtactttt
     2161 tttcaagaaa atgctgtcgc tgtcacgcga cttccatcga atgtcacatc aacagctgat
     2221 gtaaaagtca acgatagtga ttatggggcc atcgctgaag ctggaggaaa cgataagata
     2281 actatcagaa atacattgaa ggtcgagggg gattttcttg aatataagaa aatgcagact
     2341 agtatcgacc tcccacaaga attaactatt cccgagcata tcttgatcaa tggaaatcaa
     2401 attgatgcca aagatatctt gataaccgga aatcgagtca cgctaaatct gacagattat
     2461 tttattgctg gagatacgtt aataatcgaa ttggagacaa ctttgcagga agtaattcct
     2521 gaaaaagtac tcactatgca ttttgaatac cttgaaaacg ggaatttgat tgagcaatcg
     2581 aatgaaataa acctaaagat cgcaaagctg aaggagatag atatccatgt ttattataaa
     2641 gataaagaca gtcagattga tattgctgaa atgaaaacaa tcactgggaa aataggagag
     2701 aagtataaag aaactcctat tgatattgaa gggtatgttt ttgactcgga ttcagcgaat
     2761 acagaaggca tcttctccga aaatacagag gatatctatt tttattacag aaaaggcaaa
     2821 ctttatctct caactgctcc tcaatatgtc aattttggaa aacataaaat atccagtaga
     2881 ccattaagtg aattcggaca gttttcaggt gggttgaaaa ttatagatga acgggctttt
     2941 ggaagttgga aattacaatt gaaacaagag aaagttctta caaatggaga ttttgagctt
     3001 ccagaagctc tttcctttgt tacctctgaa aatacaacgg ttatagggac atccgctgtt
     3061 acgattttcg aaagtgatca aaagggtgaa agcgatttgt cctctctttt agattctaca
     3121 gctcatcgtg gaatcagaat agatattcct gtagaaaacc aaagaatcgg aacctttgag
     3181 gggaaactat cttggatact tgcagatgtc ccaggaaatt aacaggaaga gaagaatata
     3241 acaaaattat tgagtttcaa gaagggcaca ggtaccatag gctcttgtgt cccacagaga
     3301 caagattttg ttatagtctt ctcttacata tgattaaagg agcgattttc ttgagaccaa
     3361 aacaacaatt atttataaca ctcctagtta ttttaggggc actttttaac aatatatcag
     3421 tgtcagctag tgcatttaat ttttcagtga cgccgataac ttcagagaat caaatcgaca
     3481 agagaaaaac ttacttcgat cttcaacttg atccggatca ggaagtagaa gtaaaagcgg
     3541 aattgagaaa tgatacagaa aaagaagtga aaatcgatat ttctgtgaat agcgcaacca
     3601 ccaattcaaa tgtgatggtt gaatacggaa aaaacgagat tgaaaaagac atgagcctga
     3661 tctttgatct ggtagattat gtgagctatc cacagatagt gacgctaaaa cctaaaagtg
     3721 tccaaaccgt tgcttttcat gtcaggatgc cgaatgagcg atttgatggt gtattggctg
     3781 gtgggatcac attccaagaa atccagacgg aaaaggacca aacagaaact aaggatcaaa
     3841 gcttatcgat tgagaatgaa tacacctata ttattgcttt gttgatgcaa cagacactga
     3901 acgaagttgc tccgaacctt actcttcatg aagttaaacc agatcaaatc aatgcaagaa
     3961 atgtcatctt agcaaacgtt caaaatgatc aaaaaaccta tatcaatcaa gtagttattg
     4021 aaacaaaaat tacaaaaaaa ggacattctg aagttttata tcaagaggaa aaagaaggat
     4081 tgcagattgc gccgaataca aatttttctt ttccaactgc tttaaatggt caaccgctaa
     4141 ccccaggtga atatcacctt acaatgactg ttttaggaaa tgaaaatgaa aatggaaaat
     4201 tttcgagaaa aaaaggaaat actacgatca actataccaa tcaatgggta tttgaaaaag
     4261 attttacaat agacggaaaa gtagccaagg aattgaacac gacggatgtt acgattaaaa
     4321 aagacaataa ttggatttat atcttagtag cttcactttt cttattgttt attttattat
     4381 gcatactttt gtttgttttg agaaaaaaag aagagaaaga aaagtaaggt taagataaca
     4441 aatagtaaat ttttattatt gtaggatggt t
//
LOCUS       DARBDV010000144         4461 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000144 DARBDV010000000
VERSION     DARBDV010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.150"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            574..1008
                     /locus_tag="SCM56_002764"
     CDS             574..1008
                     /locus_tag="SCM56_002764"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111491.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2298..3221
                     /locus_tag="SCM56_002765"
     CDS             2298..3221
                     /locus_tag="SCM56_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG1111492.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3237..3776
                     /locus_tag="SCM56_002766"
     CDS             3237..3776
                     /locus_tag="SCM56_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEG1111493.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1664 a          667 c          758 g         1372 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacagagaa cttaaattga attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagaaacg gataaataat aaaaaagact
      181 gctcttccga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      241 tataaagcgc taccaaaaat ttatcatatt ttatgaaaaa taacaatcca gtaaaaaatg
      301 actggtaaaa attcacaaag aaacggaaag aagcaactgc agaaaatcaa gccttaaaaa
      361 gagcttttta cctagaccaa ttaaaaatca caaagtgagc ccctgtatcg gttgatacaa
      421 gggctacttg gcatactgta cattatgtct actaaccttt aaaataacac gttttataaa
      481 ataaaccctc ttgacaatat ttaattttct gattattcta aatatataca gaaagcgctt
      541 tcctaggatt ttatatctaa ggaggaagta acaatgaaaa ataaaattat taaaattatt
      601 acttgtctat ttttgttcgg tatgattgtt ggagcaagta ctccgttaaa tctgtttcct
      661 tttagtgcta gaggtataaa agtggaagct gcaagttcac gatataatca taatcatcgt
      721 ggatttactt gctataaaat gaactattat gttactaata aaatgtgtag agatttgaaa
      781 aaaaattata ataagctaaa gactcctgct caaattgcat cttttattcc tattggtgga
      841 gttggaacat gggttattac aaatgctttt agtagtgctg ttgatgcaat gaatgtcttt
      901 gtaagagctg caaatcaagg aaaaggtgta caattaacat ataatgctca ctttagcaat
      961 acaacatcat atcaatacaa tgattacgct cgttatgtaa taaaataatc agaaagtgaa
     1021 actatctcat gaataaaaag attttagctg taattatttc aataatagtt ttaatcacta
     1081 tgttcttcat atttaggcta attttcaata taagcttaca aaaaagtatt ctttatctaa
     1141 ttcctatagc atttatctta gcaattttta gaagtattta cggaaataaa aactctaaat
     1201 agctaaaaat aaatcatact aaattttaca aaaagtggct agcattttct agccactttt
     1261 tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg agcttgcgcc cctttgacaa
     1321 gcggttattt gcaccaaata cggaggtatt tggctagcaa atatagagct tgccaaaccc
     1381 ttttagtgag ttttgtagaa agtaccttta aaggtacttt cacattacga acgatacgtc
     1441 cgtaggacgt actgaaaggg tgggacttat gagcaaatta atgcaaaatt tggctagcaa
     1501 aaaagctaaa aaatatatgc taataatcga gcgaaataaa cactttttca agtgagcgaa
     1561 tccgccatga caaagctgaa gcagtttatt tcagctttgt catggcgtgt gccgactttt
     1621 ttaaaagtct aggcacacga gcaaatatct ttatcaagct ctaatttgcg ttttaagacg
     1681 tttgaaacgc ctaagtatat aaatatataa gaataggttt aagagtgctt aaatcgaatt
     1741 ttagagggca tttagacaat tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg
     1801 ttttaagaac aagataaggg atttatacta ggactgtctt aaaatgtctt aaacgaacgc
     1861 tcagagcttc tcaaagggtt ccttatgaac ggcttttagc aaaggagccg gaggcgactg
     1921 cgcagtataa tgtgagagca gagcgatcac acctttttag cgaaccgtag ggagcaaggt
     1981 ttggaattgg cagttttgat aaattcgcga acctgtttag agaaaaaagg agtgattttt
     2041 taggcgggaa aattagcctc ccctcttttt atatctcttt ttatatctct ttttatatct
     2101 cttttatgtc ggctgtgtcc ttactctccc aagcaattca gagttttttc aagtaggttt
     2161 tctacgcttt tcaagtaggt tttctacgct tttcaagtag gttttctacg cttttcaagt
     2221 aggttttccg acattattga gtatttttat aaagtatgtt acttttattt catactttat
     2281 aaaagtagga gaagcttatg gcaaatgaaa ttgtaaaata tgaaaataga ttaaatcata
     2341 ttcctctgag aaaatttaat agtcgtgaaa tgaacttatt tttttcaatt gcttcacgtg
     2401 tacgtgaaaa aggtgtagat gagattattt tttcatttca cgatttgaaa aaactaagcg
     2461 gttatacaga acgtggggaa caatttataa aagatcttag tagcacgtat gacaaattat
     2521 taagtattaa tgcttggaat gatgatggaa atactttaac taaatttgtt gctttcacaa
     2581 aatatagcat tatacgagat aaacaacttg ttataatttc agttaatcca gaatttaaag
     2641 gactttttaa tcaattaaag aactggacaa gatttaatct agagcaattt actaatttgg
     2701 atagcacata ttcaaaaact atgtttcgat tattaaaaca atatcgaact caaggctgga
     2761 ctgaattttc caaagaaaaa ttttttgatt tactagatat tcctaaaagt tattggaata
     2821 gtccgtcaaa tattgacaaa tttatcttaa ggccaattaa agaagaactc actcccttat
     2881 ttaaaggatt aactattcgt aaaaaatatg gtaaaggtcg aggtaaacct gttatcggtt
     2941 atcgttttac ttggaaagct gaaattaacc atgctgacga tttttccaaa gggaaacaag
     3001 aagatttacg tataaaatta tttaatattg atcacaatgg agaattaaca cgagaagaaa
     3061 aatggcgcgc taaagatcga atactaaatc ttcccttagg cactcatgaa gctgatttta
     3121 aacaacaaca acaaactgaa gaaaaacagg cagaaaaacg agaactttca aacaaactca
     3181 aacaagattt actaggaaat ttacaaaact tattcgatta agcttaagga gtatttatgg
     3241 cagataaaac aatcaaagaa cttgcggaag aattaggagt tagtaaaaca gcaattaaca
     3301 aaaaagtttc tgacaaaaat cgaaaacttt ggtttgcaaa aaacggaaac aagtttgtga
     3361 ttaacgaaac cggtcaaaaa gctataaaat caatgttttt agtcgataac gcaaacttaa
     3421 atcgaaaact agttggcgaa aaatcaaaaa ccaaaaacca taaaaacacc aaccaagttt
     3481 ccgaattaaa actcctttct ttcctagaaa aacagttaaa tcgaaaagat atacagctac
     3541 aagaaaaaga taatcaaata aaacaaatgc aaaaattact cgaccagcaa caacaactca
     3601 ctctacaagc gaaccaacaa attgagaacc tacaagaaca gctccattta acttatacag
     3661 aagaaactcc aacaaccgaa tcaactgcct tttctgaaaa aaaagcagac aatacacaac
     3721 cacttattga aaaaaagtgg tggcaaattt ggaaatctaa tagaaccaat ctctaaaatt
     3781 ttctagaagt tagttcggat agtagaggaa aaagttgtac tacctgatgg ggagatcact
     3841 caaattttta tgagaaagaa aaaatagttt acaactgtaa acaatcaatg tttatagttg
     3901 taaactatca aactatatgg tgacttattg cttaaaatag gttgaaatct cactcaattt
     3961 ttggtggaaa aacaaaataa attctaatcg attttgttga aaaagtcata aaaaaacaaa
     4021 tgtgatttta acaaaatttc aaaaaaatcg tttgaaattt cgtttatttt ttttgagaat
     4081 tttgatcatc gtagggtcat tgtagaatca ttatgagatc atcgtgggat catcgaataa
     4141 agtatgggaa aggcttgcgg tgcttggtat taagggcatt ctccgcctcc caggttcttg
     4201 accattttct cttataagtg ggatttttac cacgttgaga gaaaatggtc aagaacctgg
     4261 gaggcggaga aaacatacca agcaccacaa gcaaaaaaag ttagagtaac ttccaaaaaa
     4321 gaaaaatttt ttagaacaac ttttgaaaat ttaattttga tttatatccg ttcaatttaa
     4381 acatttgata aactgaaggc tagtatttaa aatgtgtata caagtggtag cgttaaaaaa
     4441 agtggaggta taacctatgt t
//
LOCUS       DARBDV010000145         4432 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000145 DARBDV010000000
VERSION     DARBDV010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.151"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(543..2048)
                     /locus_tag="SCM56_002767"
     CDS             complement(543..2048)
                     /locus_tag="SCM56_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG1111494.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="SCM56_002768"
     CDS             complement(2045..2731)
                     /locus_tag="SCM56_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1111495.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="SCM56_002769"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="SCM56_002769"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEG1111496.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1239 a          919 c          729 g         1545 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat at
//
LOCUS       DARBDV010000146         4425 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000146 DARBDV010000000
VERSION     DARBDV010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.152"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            690..1658
                     /locus_tag="SCM56_002770"
     CDS             690..1658
                     /locus_tag="SCM56_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEG1111497.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="SCM56_002771"
     CDS             1669..2484
                     /locus_tag="SCM56_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HEG1111498.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="SCM56_002772"
     CDS             2552..3262
                     /locus_tag="SCM56_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1111499.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="SCM56_002773"
     CDS             3298..>4425
                     /locus_tag="SCM56_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEG1111500.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DARBDV010000147         4394 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000147 DARBDV010000000
VERSION     DARBDV010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.153"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..91
                     /locus_tag="SCM56_002774"
     CDS             <1..91
                     /locus_tag="SCM56_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEG1111501.1"
                     /translation="GLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
     gene            complement(149..1369)
                     /locus_tag="SCM56_002775"
     CDS             complement(149..1369)
                     /locus_tag="SCM56_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEG1111502.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1389..2000)
                     /locus_tag="SCM56_002776"
     CDS             complement(1389..2000)
                     /locus_tag="SCM56_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEG1111503.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(2043..2975)
                     /locus_tag="SCM56_002777"
     CDS             complement(2043..2975)
                     /locus_tag="SCM56_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEG1111504.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3396..4256)
                     /locus_tag="SCM56_002778"
     CDS             complement(3396..4256)
                     /locus_tag="SCM56_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1111505.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1257 a          881 c          726 g         1530 t
ORIGIN      
        1 aggattatat aagaaaaccc gaaaagaagg cactctcttc gggttttcgg tctgtactga
       61 aatcaaggta ttattgggaa tcccagctta aatcatagat accgtaaggg actttattct
      121 ttatttaaaa ctttgcaaca gaaccttttt attttacaga agcactcgaa actgcatcta
      181 ctgcagtttc atcgtgtgtg ctggcgctgg ttactgcatc tgtttttcca ccatgaacag
      241 atgcacttga aaccgcatct tcatttccaa caagttgttg acccgtttgt tgtaaatacc
      301 actcaatgat tggtttgaaa tcaagaatgt attcattgat tccagcatac tctccatttt
      361 catcatgcat tgcttggtaa ttatgcacaa tatatttttc tggcacttgt tttggtacat
      421 gaacacgtac cgcatctgtt gctccgcttc gcagttgttg aattacccac tctacatttt
      481 tatacgattt ttctgggtga cagttggcta atggattccc tacctgaccg ggcacacgag
      541 cagctaacat ctctttcgct tcttggcgca aattataata taaaaattgg ttattattat
      601 ctgcaaaagt taattccatc ggcatcgaat ctaaaaagta attgatttga tccactgtca
      661 ataacccacg atttaatttg acgtaagtat ttccagaaac ggcaccgact gcttcagaag
      721 ctttttccaa ccaatgatca tctgacatat caacatcggg tattgtcgta gcaattgttc
      781 ctgtcgcatg caaatcttct ggtggaagct ttttaggccc tgctaaggta gacacgactt
      841 taatgaattt tacaaaactt gctaatgtac tgcctaaaaa gtcaacagtt cgtggatcaa
      901 tcaagcgatc ttcttggtca aatgcttctt ttgcttttcc caataaaaat tcatttccgg
      961 gcatcactaa tgcattcacg cctggtgcgt ccaaaatttg gcgcaagtgt aattgcgcgc
     1021 gtgaagttcc ttgatcgtag taagaagcac ccacaaccat tacaggttta ttttcaagtg
     1081 gatggatttt aaatgatagc cattcaagtg cacttttcaa tcctgctgga accgtatgat
     1141 tgtgttctgg cgtagcaata attaccccat cagccattaa gattttgcga tttagctgtt
     1201 gaatagcagg actatttgtt tgatcatcat cttgattgaa cagaggtaaa tctttgattt
     1261 caatgatttc taaattaaat agttcatgat attgtttttg gataaatgtt aataatttac
     1321 ggttatatga aagttcagct gctgatccga cgatacctat taatttcatc tttgtttgct
     1381 cccctcgatt aaatcgtttc ccatgaaaag ctttcggctt cacgtttatt tgcttggtgc
     1441 gcatgttgca attgactagt gattgtgaca aagagtaaga aatctttgaa tagttcacgt
     1501 aattttgcga cttgttcttc atcaatgagt cgtccttgct catcaaaggc ttggagtgaa
     1561 tgacccaata aaaattctga gcttggcata attcgtgcct ttaactctgg tgaatccaat
     1621 atttggcgca aatgtgcttg tgcgcgtgaa gaacctaacg tgccataaga cgctccggta
     1681 atcatcactg gtttatccac aaacggaaaa ataccatatg aaagccaatt taatgcgtta
     1741 atcaatgaag caggaaccgc atggtcatac tctggtgtgc tgatgattac gccatctgct
     1801 tgttcaattt ttccagcaat ttctttaaca acatttggta gttctgtgtt ttcaggtttg
     1861 ttaaatagtg gcagctcctt gatttcgaca atttcgattt ctgcctcatt tacaaaataa
     1921 tttttcataa actgtaataa ttcacggttt gttgaccgcg ttgaatttgt tcctactaat
     1981 ccaataaatt tcatcttcat tctctttcct ttctaacatc gatttctgac tgttcgcacg
     2041 tactaaattt tttcaaatcc ttttgtatac aagaattgat tctcttttgt tacaactgct
     2101 ccctcgatac ccggctgttc ttcaagcgct ttcaacactt ctaaagaaga tgctccaaat
     2161 aaacgtgtag tccagatttc tccatctaca gaagaatcag acaaaatcgt caggcttgct
     2221 acgtcatttt taactggaaa gccagttttc ggatcaatga tgtgatgata cactgacgaa
     2281 ccattttgta gataacgttc gtagattcca gaggtaacta aagattggtt gtttagttta
     2341 accaccgcaa gcgactcttc acgtgaacct ttaggatttc gcaaaccaat acgccaagca
     2401 ccatccgaac gtcttggtga ttcaccaaaa acaacgatat ttcctcctaa gttaactaaa
     2461 gcaaacgctg catttcgctc tcggcaatgt gtaattaatt tatctgcaat gtaacctttt
     2521 gctaacgccc ccaagtcaat catcattcct ggtttggtta aaaaaacggt tttcattttt
     2581 tcatctaatt gaatttggtg tggatccgtt agcagaagta actcagttat ctctgctttt
     2641 gttggaacca atgcatcttc aaatccaatg tgccattttt gtacaaccgg accaatagca
     2701 atattcagat aactatttct tgcacaacta tgtgcttttc ctaatttgat gagctcaaaa
     2761 agttcaggat gtacaatcac agcttgtcgt ccagcagctt gattgatggc cattaattca
     2821 gaagttgcat catgtgcgct gaagcgatgt tcataaacgt gcaaccattc gcctaactct
     2881 gctagtaatc gactaggctc ttcatgccca acttgtaatg taattgttgt ccccatcatt
     2941 tgccagattt tcgtttcttc gatcacctga ttcattttgt accttcttta cttgaactac
     3001 ggattacatg agtaattagt ttttattttt gttactgttt tcacaaccag atgatacgcc
     3061 tgctagattt atttgtcaac cgattttata tattttttta tataataagg taattttaat
     3121 ataaaatttt atattgtaaa aaaacaaata agggaaacaa acaacacatt tttgtgtgtt
     3181 gcttatttcc ctctaactat cgttttctat ttgatggtta acctacttaa aataaatact
     3241 gcaacgacaa acggagatca agcatgcttt gtaacgtatt ttacgataat tacgacttta
     3301 ttttattact ctatcttgct gccatacccg tctatcccta atcatctttg gtgaattttt
     3361 tcaaagttag cataacaaaa ataagtattt tttccttagt taatcaatag tatcattttt
     3421 ttcatatgat ttaattcatc aattaatttt ttgtttttat taattttagc aaaagctagt
     3481 gcatcttgaa tcaattcgag cacttctgcc ttcgacttat ttttagcctg tgcgttcttc
     3541 gctaattggg cagcagctcg tgctaagtaa taagtcaaat ggttatccga acaaatatcg
     3601 attgcgtaat ttagtagtgc atcacttgtt tccaaatcac cgtcttgcgc aaaaaaaaca
     3661 ccacaatgaa agacgatatt taacacccgc cagatatctt ctgttgtacg aatcggatac
     3721 gtataaattt tttcaaacac tcgattgaaa taaacttctg ctttttccaa ctctttttct
     3781 ctagcataga ccattccaat acctgtatat gccaataaac gaaacaacgt gttggctgtt
     3841 tctgccatca acaactgatc aaaatggaac aacacatccg taattggtgc attcgtaaaa
     3901 atcatttcaa atccttttag atagtagtaa cgtgcaatca gctcagactc gtcaattacc
     3961 ttgggatcaa tggctgctaa aatctgtgtc aattgttcat attcactcgt aatcagtgaa
     4021 aactctgctt tttctagctg ttcatcaagt tgtgtattga tcacgttcat cgaagaaaaa
     4081 agttcaacta acggaagatt gattcgatta cataatttga ttaaaatttt taacgaaggg
     4141 acttgaccat tgttctcaaa tttacttaat gttgcttgtg tacaaattcc ttcagacact
     4201 tctttttgag ataagttggc agcctttctt gccgtaataa atttttcaat gttcattatt
     4261 ttctctccaa tcagacctca cacgaaaatc atttctgtct cgattttagc atgttttctt
     4321 tgggaatgct ttaggttctg ttgcaaagtt ttaaataaag aataaagtcc cttacggtat
     4381 ctatgattta agct
//
LOCUS       DARBDV010000148         4348 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000148 DARBDV010000000
VERSION     DARBDV010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.155"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(387..1853)
                     /locus_tag="SCM56_002779"
     CDS             complement(387..1853)
                     /locus_tag="SCM56_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1111506.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
     gene            complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="SCM56_002780"
     CDS             complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="SCM56_002780"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEG1111507.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT         1309 a          927 c          697 g         1415 t
ORIGIN      
        1 aataaatggt gttcaatcag ctgaccttca gcattagctc aaaaagcggc aaaatatgag
       61 gagctatttt tccgtttgtt tctctaatac tcaggtcaaa tcgctgattt tacaacctct
      121 tccttaaacg gtgttccaaa gcttgcatct gcggtaataa gcccagccaa acgaaaaata
      181 tgaggggcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
      241 ctctgattca cggtgttcca gaagtagctt cttcgaaaaa caagccgaaa cttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttgt aacaagtatt tctattttaa ggctgaacca ctgtttctgg ttcagtgatt
      421 tttttgtttt tgtctcgacg agcaattgct ggcaagatca tacctaataa gatcaagacg
      481 attggtgtga tgatattcgt aaataattgg aaccaccatg ctttaggatc agcagtataa
      541 tctacttttg gcaccattcc taggatacat gcaaacgcag tgaaggcaaa gcaccaagca
      601 ccaaagataa aaccgatttt tggattttta acaaatttat attcagaatt gaatttctta
      661 taagctttat tcaacatcat atatgccaag aacacccata ggtaacgcat tggcatgaca
      721 actgagttca agttcgtcaa ccattttact aattcattca tatctttgat accaaaaatc
      781 ggcaacaaaa taatgattcc tactagtata cctgtcagaa gataaccatt caccaatgta
      841 cctttacgat tacgtttacg tagccagttt ggtacaaatt ctggatccgc atctgctaac
      901 aagattttta gtggtgcatc aattgaaaag gctaatgcgg agatttgtcc aatcatgttt
      961 gtcaatgcgt aaatgatcat caatgagtta ccgataccat agtaattacc taaaatttga
     1021 aaagcactgt atgccccatt tgccatcaag tctttaggaa tattatcact agcaaacagc
     1081 atacccattg cgactgagcc taatacagca caaatcccta ccattgctgc taagaagatc
     1141 atccctttag gaaattcctt tgcaggattt cttgtagagt tgacataagg ggatattttt
     1201 tccgcccctc caactgcaaa tactagcatc gaaacggttg tgaaataact gaaatcaaat
     1261 gttggaatat atgttttcac ttgattcata tgtggtgtgg caaattccat cgttggattg
     1321 atgaacggtg ccgttacagc taaaagaata aataagatcg acatgacgaa catcgctgtt
     1381 ccggccagtc cgccaatcac tttcaaagta gaaagtcctt ttgttgataa atataaaaag
     1441 gcaagaaata taataagtga aataactgct accatttgta ctgacatcgt atttacgata
     1501 tttccatttc cttggactgc ccaaccacca gcaatcagaa ttgcttgagg cttttgtgcc
     1561 aaataaggaa tatggacgac ccagtaagtc caagcggcat agtatgccag tcgtttatta
     1621 gatgtcgtat tttcgaccca tgaactgacg cctcctttac tatccttgaa ggctgatcct
     1681 aattgtccaa cgatcaatgc gtatggaata aaataaatgg ccaaaatcaa tagccaagag
     1741 gtgacaactg atactccttg ctgagcatag ttattaacta cattccccat gccccaaacc
     1801 atgttaaatg caattaacgc aacagtaaac cagcgtaatt gtttgttgtt cattcgttct
     1861 ctcctttatg ataaaaattt gttatccaga tgtaatcata ttcaattttt aacaaactta
     1921 caagacagtg ttattttatg tgtgtattct tttatttaat acacatcgtt ttttcatatt
     1981 ttttccatca aaaaaataag ttattttctt ttatttcatt gaccttattg aatttcaagc
     2041 tattttgaat tttctgcaaa attaaaaaca ttagttattt tataatccta tctattttct
     2101 ttacattttt cattaagaaa aggatataaa aaaaagagga cgttcattcg tcctcttttt
     2161 ttacatgcaa gcttcgcatg gcattcaaaa cagctaatac tgtcacccca acatctgcaa
     2221 atactgcatc tcccattgat gcaaaaccaa aagcacctaa ggcaagtacc aaaattttta
     2281 tcccaatagc aaagacaatg ttttgtttga cgattttcaa tgttttcttt gctaaatgga
     2341 ttgcttctgc aatcttcgat ggttgatcat tcatgatgac tacatctgct gcttcaattg
     2401 cagcatcgct tcccaatcca cccattgcga ttcctacatc tgctcttgct agcaccggtg
     2461 catcgttgat tccatctccg acaaatgcgg tctttttatt gtttctttgt aagatttcct
     2521 ctaaccgctg cactttatct tcaggaagca attcgctata tatcttgtcc acgcctactt
     2581 gcttcccgat atgatcagca atttttttcg agtcgcccgt caacataact gtgttcttca
     2641 caccagcctg ttttaattga accaatgcgt ctacggcatc ttctttcaag gtatctgcaa
     2701 taacaagata accactgtaa ctttgatcga tcgctagata aagcaatgta ccgatttctt
     2761 gacttaaagg aaaagagatt tcaaatcttt ccatcaattt gtgattacct gctaatactt
     2821 gtttgccatc aacttcagct aagatccctt cacctgcaaa ttcctggata ttagttgttg
     2881 gtaaaagcgg acctgcgtag ccttctaaaa cagattgagc aatcggatgt gtagaaaact
     2941 gttctgcact cgcaatatat tgcatgaatt tctttggatc gatttttgta tcgatcgtct
     3001 ggatcgaaaa gtcacctttc gttaatgtgc cggttttatc gaataccact gtctcggttt
     3061 gcgcaagcaa ctccaagtaa tttgaacctt ttactaatac accgatttta cttgcgcctc
     3121 cgataccgcc aaagaaacta agtggcacgg aaatcactaa tgcacatgga catgagatca
     3181 ctaaaaatgt caatgctcga tagatccatt cagaaaacgc cgctccagaa acaacaagtg
     3241 gaggtaaaac agctaataaa acggccaatc ctacaacgac tggtgtgtaa tagcgcgcga
     3301 atttagtaat gaaattttca gctggtgctt ttttactgct ggcattttct actaattcta
     3361 ataatttgct gatagtagaa ttttcataag atttttctac ttgaactaaa aatggtttat
     3421 ttttattgat aaatccgctt aatactgttt caccaacata tacagattta gggactgatt
     3481 ctcctgtaag tgctgacgta tcgactaatg attctccttc gatgatcgtt ccatctaacg
     3541 gtacacgttc accaggcttc acaagtacaa actgaccgat ctgcacgtct tcagggttca
     3601 cggtcatcaa tccttccgtc gtctgcacgt gagctgtttc tggacggatc gccatcaatt
     3661 cgcgaatcga ttttcttgaa tgactgactg ccaatccttg gaaatactcg ccgatttgat
     3721 aaaatagcat cactgctacg gcttctggat attcaccgat caacatagcg ccaatcgtag
     3781 caacagacat aagaaaattc tcatcaaaga tttttccttt gacgatattc atcactgcct
     3841 tcttcactac atcatatcct atcagcagat aaatcaataa ataccctatc aagcgaattg
     3901 gttcattaaa atcaaataat agcaataaag cgatacctgc aagggaaaat aaaatccgga
     3961 acagcgtatt tctctctttt tcatttccat gttcagatac tttttcctta tctatttctg
     4021 ttactttgac atctggttct aattccttca ctactttttt tgtttcttgt tcaatcgtag
     4081 ccaaatgatc ttcctctgcg tcaatcgtta gttttccaga agtgaagttt accgttgctt
     4141 ccttgactcc attgatcttc tgaactcctt tttcgatttt cattgcacag ttcgcacaat
     4201 ctaaaccttc taatctgtaa cttttcattt taatgacctc ctcgcctcaa catatgaaca
     4261 tctcttcata actaattata aatgaatggt tattcatatg tcaaggtatc cgaaagaaaa
     4321 aatgaaaaac atctgcatcg gaaaattt
//
LOCUS       DARBDV010000149         4036 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000149 DARBDV010000000
VERSION     DARBDV010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.156"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(242..391)
                     /locus_tag="SCM56_002781"
     CDS             complement(242..391)
                     /locus_tag="SCM56_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111508.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            853..1065
                     /locus_tag="SCM56_002782"
     CDS             853..1065
                     /locus_tag="SCM56_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111509.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1343..3142)
                     /locus_tag="SCM56_002783"
     CDS             complement(1343..3142)
                     /locus_tag="SCM56_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEG1111510.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3266..3862)
                     /locus_tag="SCM56_002784"
     CDS             complement(3266..3862)
                     /locus_tag="SCM56_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEG1111511.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1449 a          711 c          689 g         1187 t
ORIGIN      
        1 atattaaata ttatacagat aataacggaa tattatcatc gaaacagcta acaaaattgt
       61 cataaaaaac gagatggttg atattagaac ttgtgttttt gtttaggtct agagataaag
      121 agagacaatt tccaaatata tgtaatgaat acaaaaagac gtttgtcgcg gattaccgtt
      181 acaaacgtct tttattgatt ttccgagatc tcatcagtcg ccatttggaa tggttcactc
      241 cttatctaga ttcgtttaat gctcggactg cttgttcgat tgtaatacca aacgcaacgt
      301 tattttcgtt ttgtgcatca atcgccataa aacggttaga agttgtatca aaagctacgc
      361 ggtattgcat atttgttttt gtaaacgtca taaaattcat ctcctttgta atgtaattta
      421 gttcttgaaa gtttccttta aactttcgaa cttataccaa gtttagcatc gtacgttttt
      481 tgatgcaatt aaaaagcatt tatcattttc cttatttatt ttttcttaaa tggtttagct
      541 attttctttt aatccgataa gtagcccaat catcaaggtt acaaatacta ttgcttgaat
      601 ataaatcata tgctcacctc tttataaata acttagcaat ataaagaaaa aaacgcacga
      661 acaacatagg aatataaaca aacgtttttg tcacaacgtc taagatcgtg ttaaaaaata
      721 gttatctaaa aagcagaaag atactcaaaa taaaaaataa aaaaatggat aaaaacgttt
      781 tcaaatacaa ggtaatttgt tattataaaa taaagaaaaa acaaaataaa aataaagaaa
      841 agagggaatg aaatggtaaa agcagaagta ggaagatctt atatatgcaa accgatcgga
      901 gtatctcatg aactaatcgg ctgtattgaa cgtacatata tcaacacggc tgtcgtgtat
      961 gttgaaagtt atgaccaacg agactatcaa cttatcaatg aaagcaagta tcgcatgctc
     1021 gtaaaattct gtgatattag cgcacctgct gaattggttt cttaaacagt atttttgaga
     1081 attcgcaaga actcccttat aatataaata aagacagaag ggagcgagaa gatatgccaa
     1141 aaagaaaaag ttcagggaaa atgggaatta ttcttgcagt gatctctgca attcctgcta
     1201 ttttagctat tgttcgtgcg atgaaggaat caagagaaag ttaagcacac attatgagcg
     1261 tcaaacaaaa aacgcatata cctgcggtgg caggttatgc gttttttgtt gtataaagac
     1321 ttttttcatt aacgattttt gattagccga ctcccaaaac aaaatttagc agtttatctg
     1381 aataagtgat ttcttcatct gtttgtttca cactttcatt tagtagtccc atctcatcgg
     1441 ttatgatacc atccacacca taaaacatca tgcgactcat agcatctgaa tcgttgattg
     1501 tccatacata tacttctttt ccgtcttggt gggcagaatt aatgaagtta cggttgatcg
     1561 tactatattc cattgtcaga aaatcagcag gcgtcacagg gggcccaaca atattgaaag
     1621 gtaagatgta gcccacataa aagtttggtt ctgcttcttt taaatctgaa acggtttggt
     1681 acgtcaaact ttgcaaaata tgatggttct ctaagataac tttgcggtat ttatggacaa
     1741 atcgttcgac aaggtcatcc gaatcttttt tagaggtctt gatctcaata agtaattttt
     1801 ggtttagctt atgtgctttt tccaaataag aatcaaaaga acaaacaggg gcttgcactc
     1861 cgttttcagt aactgtaaga tttgtcaatt ctttcaatgt aagatcctgt ggtgttccat
     1921 ttacccctgt taagtgacga agatttgcat catgcatcac tacgaaatga ccatcttttg
     1981 tttcctgaac atccatctct acataatcag gatgatagtc tctgcttgtt ttttcaagtg
     2041 cttctaaagt attttgcgcc ccattttggg tactcacacc tctgtgagag acagtcactg
     2101 gttttgtctg aacagctgaa gtcagataat ccatattgta aagacagacg ccaataccga
     2161 aaaaaactgc aaaaagagtc aaaccagtgt ttttcagcgc agagaaacgc aagttggggg
     2221 cttgtgggat aaaccactta gggatctcag gcaagaagcc ttcgtcatcc atgaaatcaa
     2281 caatgatata aaaaatccct acggtagaca tcacgatatt gaataataaa atcccttgta
     2341 aaagcgtcat agcaaacaca gcgctgatca atgcatagtc tgggaaaaat tgttcaacca
     2401 tactttgtgc caaaataaca acgatatatc ctaaagaaga aaggagtaaa atcgtaccgc
     2461 caatcacaat aaattggccc gtgatggcca aaagacgcga tttggtcaat gaccagcttt
     2521 cccgaatggc tgctcgaaaa ggacgatctc gtagaatcat ttccggtaaa gcaaaaatca
     2581 gtcgaatccc aatatagcca aggaaaatat aaacgagtaa aaaggaactg acgatgatcc
     2641 aacgattagc aaaaataaaa tccataatga aagcggggat ttttattttt gacagtagat
     2701 cagaattaaa acttaatccg ctgatcggta gaatcaataa aaaataagca aggaaaaaca
     2761 aaaaagtaat gggtctcact ttcttcagct gaagtatcgt caagtgtaag agttgcttca
     2821 aagagattgg ttctttcttt ttaataaaaa aaacactcat caaaagaaaa gtaaattcga
     2881 aataaactaa cagtaaaatc gtcatcaaaa ctacgattaa cgagagtaga accccaggat
     2941 gttgagaaaa aattgaagga atattgtcat aagacaaata gtcaattgcc cctcggtgta
     3001 aaataaaacg ggtcgtactg ctcaataagg gtaaacaaat aaataaaata aagccatgca
     3061 tcagcaagac atcacggaaa taagcttgtg ttccttttaa aaagtcccaa gcattaccta
     3121 agctacgttt caaataattc atcctatcac ctgctcttca cattgtagcg caaatctaat
     3181 gaaaaaggaa agggtgcagt gtgttttgca gaccctttcc cagatttaac attgtcaatc
     3241 aatgactaat tttacaaaat aaatattatc caagaagacc acttagatat tgacctccat
     3301 aaatcaaggc catactatat aagaaaatcg acagtggttt agcaatcaaa atgattagtg
     3361 tatatcgttt cactgacatg tttgtcaatc cagccatcaa acataacgca tcgtcaggag
     3421 caactggaag aaaaatagct agagcaaaca gcttctcaaa acggttttga ttatccagcc
     3481 agccaatata tttgttatat gtcttatcgc taatcatgga aagtatgaat ggctttccat
     3541 aacgacgccc tagcagaaaa atgataatcg aaccaatgca gattccaata tagttataga
     3601 taaatccagc ataagggcca aatatcagga caccagctgc agtactgatc cctccaggaa
     3661 tgattggaat aactacttgc aggatttgga tgaagacaaa aataatcggt cctaaaatga
     3721 tggaatcacc tactaatcct cttaacgcat tcaaatcttt gaaaacacct aaattaataa
     3781 aataaatcgt cagcgcgatt gataggccta atccgataat ggatatgaaa tttatcagtt
     3841 ttcgagaggc agcaatattc attattcatc aactccttag aggttctttg ttaactatac
     3901 tttactagaa aagcaactaa ctgcgccatt ttttgatatg acagaaaaca aacaattttg
     3961 taagttcatc ataagtataa aagattgatc tcaaaaaaac atccaactaa agacttattt
     4021 aataacttgt tttttt
//
LOCUS       DARBDV010000150         3930 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000150 DARBDV010000000
VERSION     DARBDV010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.157"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            174..518
                     /locus_tag="SCM56_002785"
     CDS             174..518
                     /locus_tag="SCM56_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEG1111512.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            519..938
                     /locus_tag="SCM56_002786"
     CDS             519..938
                     /locus_tag="SCM56_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEG1111513.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1030..1380)
                     /locus_tag="SCM56_002787"
     CDS             complement(1030..1380)
                     /locus_tag="SCM56_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111514.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1346..2566)
                     /locus_tag="SCM56_002788"
     CDS             complement(1346..2566)
                     /locus_tag="SCM56_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HEG1111515.1"
                     /translation="MLSNTPTDNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILT
                     LLSVLLAYRHKVILNGPLFASILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQ
                     YIMIGLFGSALSPVVSYITFGMRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGF
                     TLYNIGFTSGLIAMTFTAVLRLFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFY
                     YNSFRLLGIREIFDSSGKLTTDFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPV
                     IGAILSAVGFSAFGCHLKNSFPILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPIS
                     GFYGSFYGVIAGVLHIALVHNVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKE
                     KKTLGKRIIKKNHR"
     gene            2932..3498
                     /locus_tag="SCM56_002789"
     CDS             2932..3498
                     /locus_tag="SCM56_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1111516.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3643..3730
                     /locus_tag="SCM56_002790"
     tRNA            3643..3730
                     /locus_tag="SCM56_002790"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3675..3677,aa:Ser,seq:cga)
BASE COUNT         1436 a          677 c          763 g         1054 t
ORIGIN      
        1 aaataaaaaa agcgttagca actatgctta aaaagcatat cgactgaata aactaagcat
       61 tggacaggct gaaagactac atttaaaatg aaatgcgaag aaacaataga aaataaagtt
      121 gccttagaac catagttgaa tcacctaaca gtcgtgaagg gagaataaga gaaatggcag
      181 aaaaacaaac cgcaggacga gatcaattag gagattttgc accaaaattt gcagaattga
      241 atgatgatgt tttatttgga caagtatggt ccagagaaga attatctgct catcagcgta
      301 gtatcatcac catatctgca ttaatttcag gaggaaattt tgaacaattg ccagctcatc
      361 tgaagattgg caagcaaaat ggcattacta gtgaagaaat agcagaaatc attacccatt
      421 tagcttttta tgtaggatgg ccaaaagctt ggtcagcttt tagtttagca aaagaaatct
      481 atttagaaga aaatagcgaa gataagaaag aggcgtaaat gacgacaaaa cacgaagaaa
      541 tacacaatgg cgtgattttt ccagcaggtg aaaaaaacga agcttacgct caagtattta
      601 ccgggcaaag ctatctgaat atgttagtga atgatcccga agtttctgtt ggtgtaggta
      661 atgtaatttt tgaaccaggt tgtcgcaatc actggcatgt ccataaagac ggctatcaaa
      721 tcttacttgt gaccggagga gaaggctggt atcaggaaga aggaaagcca gcaagacgat
      781 taatggctgg agatgtcgtc gtaacgcaca aagggattaa acattggcat ggtgcgacaa
      841 aagacagttg gtttgaacat ttggcgatca ctgctggtaa ttcagagttt ttagaacctg
      901 taacagatga agtgtatgaa ggattggaaa atcaataatg tcaagcttga acagctcctg
      961 aaagtagatt ttaatcgaac tttcaggagc tgcttttttg attcagtctt ttataaatag
     1021 aaaatagggt taataatagg aatgagaagc acgtcgaaag atcttaatat tgataatcgg
     1081 ctgggcatac atgatttcca cgtcatcaat caaactacct aacagatgat aatcttcccg
     1141 atcatgtcgg ggattaccta atagttcttg atagtagatt tccatactag ggtcccgagg
     1201 attgttcagc aaatcagcaa gtacatctaa atcttctggc tgagtttctg ccgtaccctg
     1261 tatctctatg aaaagccctt cctcagcaaa ttctactcca atacgtattt cagttatttc
     1321 atgggaatat aaataagtaa gaagctcatc gatgattctt tttgataatt ctttttccaa
     1381 gtgtcttttt ttcctttctg acagcggtaa agttatctag tatcggtaca aggatacctg
     1441 caacgaaacc agttgaaaaa ccactgttat atagatttag tcctccgtgc aaagtgctga
     1501 cgttatggac aagagcaata tgtagtacac cagctattac accgtaaaaa ctgccgtaaa
     1561 aaccgcttat gggcgcgaga cctgtcccaa acacagctgc aacaagcatg ccagtactgg
     1621 tgatctcatg aaaagttcca aacaaagaag caataaatat tccgactagt atcggaaaac
     1681 tgttttttaa atgacaacca aaagcactga aacccaccgc agataaaata gcaccgatga
     1741 caggtccatt taactgaccg ccaaccagca aaacataaga agacagcatc agaccaacta
     1801 agcctatgtt gatcaaagta gcaccaatac cagaattagc aatgaaatca gttgtcaatt
     1861 ttccagatga atcgaaaatt tctcgaatcc ccaaaagccg aaaggaatta taatagaaac
     1921 caattccgac tgtcagcgaa aaaaagccaa aaataatcca aatgagagga aagtgatatt
     1981 cattgaaaac caatgtattt tcgacgattg aatagctgaa taacctcaaa acagcagtaa
     2041 aagtcatcgc aatcaaccca gaggtgaatc caatattata aagtgtgaat ccacgatgga
     2101 aaactaaagt ttgagcagct aagggcggaa gaagtagacc aattgctatg ccagctaaat
     2161 tccccaataa aatacctatc agtaaaggaa aacgcatacc aaaagtgata taactaacga
     2221 ccggtgataa tgcgctacca aacagtccga tcatgatgta ctgagaaaaa ggtttattga
     2281 caaatacagc gtatagatac acacctaaaa tgattgagat agaattataa aggtttttgc
     2341 caaaaaaaga gaaaccggta actgttagaa tcgaagcaaa taagggaccg tttaaaatca
     2401 ccttgtgccg ataagctaaa agcacagaaa gtaaggtcaa aatccctaca tttatgaatg
     2461 cgcttccgaa accgcctaaa gcaaaataat ctgtcaaaag attgcttggc gaggtaagaa
     2521 tagttagcaa tccactccaa ttatcagtag gagtattaga aagcaagcca ataaaaagaa
     2581 aaaaacacct gtaaaaccaa gataaaaata ctttcttttc tgtgaaacga ttcgtttttg
     2641 aggatgaata taaataacgt cagtttcgtt ttccattaca caccttcttt ttcattttct
     2701 ttaactataa cttgaaatga aagatttgga caactatcat ttaggtcaaa actaaaaatg
     2761 taagggaacc cattgaattt aaccaaattt ttttaatttt gcaaaaaaaa gttaaagaag
     2821 actgaaaaaa aatttaaaat ataacgtttt gagtaaaatg tttgcaaaaa atgctaaaaa
     2881 tagggtacaa taaaacaaca tatactgcag taaagaagtg ggggagtttt aatgagaaga
     2941 aaagtctata ccaaggatca aattctaaaa gctgcttatg atgttgttgc aaaggaaggg
     3001 tttaaaggat ttactgcgag aaatattgct aaaaagatgg ggatttcgac acagccgatt
     3061 tatctggaat tcaaaaacat ggaagattta aaaaatacgt tgctggattc tatctttgaa
     3121 caactaaaag aagatgtttt ccctattgaa cgtactggtg ataaaatcat tgatctcggt
     3181 ttgaactata tctactttgc aaaaaagcat catagtcttt acatggcatt gtacattgat
     3241 gaacatgggg acggaaagaa aatgcatgat ttttcataca attatttccg tgaaatagtt
     3301 aaagaagatg aaagatacaa tcaattatcg gatgaatatg tcgatgctct gcatactggt
     3361 acatgggtag cagtgactgg cttagcgtca ttgatgtcat caaacatcat gcatccaaca
     3421 gaagaacaaa tcattgcact gatcaaacgg acaattgact cgattttagc attagataat
     3481 cctgaaataa tcagatagtg ttctatcagt atgaatttga tcggtaagtc cgttggctgc
     3541 aaactgtcaa tggacttttt tgctaaaaaa agcgatttct gttatttatc tattagaaat
     3601 aggcttgaca tttcgtgaaa aaagtttaaa aatagtactt acggaaggtt ggcagagtgg
     3661 taatgcaccg gactcgaaat ccggcgaacc ggctaatacc ggcgcgcagg ttcaaatcct
     3721 gtaccttcct tatatcaacg attagaggtt gtgaagaagc tgtcctgcat caagtaataa
     3781 gaacggccaa acaaaaatag cttttcctat tttttcgttt gattgggctt attaccgcag
     3841 atgcaagctt ttgaacaccg tttgttcgga ataaggggct gtgacaagac ttttgtcaca
     3901 gccccttatt gttgtacttt taacctacaa
//
LOCUS       DARBDV010000151         3910 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000151 DARBDV010000000
VERSION     DARBDV010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.158"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1435
                     /locus_tag="SCM56_002791"
     CDS             <1..1435
                     /locus_tag="SCM56_002791"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEG1111517.1"
                     /translation="EEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLF
                     NVLADQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITE
                     VIKKLNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQ
                     KERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDA
                     EEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKS
                     KADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANL
                     FFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDV
                     EKYAHLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKKLL
                     EAISSLQTTPENEAWLQGALEVFKHEQ"
     gene            1425..2642
                     /locus_tag="SCM56_002792"
     CDS             1425..2642
                     /locus_tag="SCM56_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEG1111518.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            2655..>3910
                     /locus_tag="SCM56_002793"
     CDS             2655..>3910
                     /locus_tag="SCM56_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEG1111519.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
BASE COUNT         1355 a          669 c          815 g         1071 t
ORIGIN      
        1 agaggaatat gacacagtaa gcaaacaaac aatgttgtat cgtgaattac tttcagatga
       61 agaatcaaaa gaagatctga ttgccacaat cgttgatatt ttgggatatg caattgcacc
      121 agagtatctt ttcaacgtct tagcggatca agcaaaacaa gcaacgttcc aattgaacga
      181 tctaaacaaa gcctttgtgc aattggcatc tacctacaat caatttaatg ggttatttga
      241 tgatgtggat ctccaatcaa aaaaactagg aacagatgag caacaacgaa atgtgacgat
      301 taccgaagtc atcaagaaat tgaatgatgt agatgtttta gaacacgatg gggacgtgat
      361 cggggatgcg tatgagtttc tgatcagtca atttgcttca gaagcaggga agaaagccgg
      421 agaattttac acacctcata tggtttctga tatgatggcg caaatcgtaa cgctcgatca
      481 aaaggaacga cgattcttta gtgtttttga tccaacgatg ggttcaggtt cattgatgtt
      541 aaacgtacgt aactacttaa cacacccaga taacgttaaa tatcatgggc aagaactaaa
      601 tacaacgaca tacaatctag caaaaatgaa cttgatttta catggtgtcg acgccgaaga
      661 aatgaatctc cgcaatgggg atacgttgaa taaggactgg ccaacagatg aaccttacac
      721 atttgatgca gtagtaatga acccacctta ttcggctaat tggtcagcag atacgacttt
      781 cttagatgat tctcggttta atcgttatgg gaaattagcg ccaaaatcca aagcagattt
      841 tgcgtttctt cttcatgggt tctatcattt gaaggaaaca ggtaccatgg ccatcgtctt
      901 accacacgga gttttattcc gtggcgctgc agaaggggtc atccgtcaaa aattactaga
      961 agatggcagt atttatgctg tgatcggtat gcctgcgaac ttgttctttg ggacatcgat
     1021 tccaacgacg gtgattgtgt tgaagaagaa tcgtcaaact cgagatgtat tattcattga
     1081 tgctagtcgt gaatttgtta aagggaaaaa tcaaaataaa ctatcggaag aaaacattca
     1141 aaaaatcctc gaaacctacg ctgaaagaaa agatgtcgag aaatacgctc atttggcaac
     1201 ctttgatgaa atcaaagaaa atgactataa cttaaatatc ccacgctatg tagatacttt
     1261 cgaagaagaa gaaccaattg atatggttca tgtcggaaat gacatcaaga agatacgaca
     1321 agaacagcaa gtgctagaaa aaaaactttt agaagctatc tcttccctgc aaacaacacc
     1381 tgaaaatgaa gcgtggttac aaggtgcgct ggaggtgttc aaacatgagc aatgatacac
     1441 aaccagaaat ccgtttccca ggatttacag atgattggga acagaggaag ttgggggact
     1501 tcgttgtaga ttatgttgaa aagacaagtg tacaaaatca atttcctatg ctaacttcat
     1561 ctcaacaaaa agggatagtt ttgcaagaag actattttgc aaatcggcaa gtaactacag
     1621 aaaataatat tggttacttt gtactaccta gaggatattt cacgttccgt agtcgaagtg
     1681 ataatgatgt gtttgtgttt aaccgtaatg acattattga tagagggatt ataagctatt
     1741 tttatcctgt atttacttta aaatctgctg attccgactt cttcttaaga cgtattaaca
     1801 atggaatcca acgacaatta tctattcaag cagagggaac aggtcaacat gttctgtctc
     1861 taaaaaagtt taaaaatata gtagcaatgt ttcctagtga ggaagaacaa caaaaaatcg
     1921 gcaccttctt caaacaactt gacgacacga tcgctcttca tcagcgtaag ttagatttgc
     1981 tgaaggaaac gaaaaaagga ttcttacaaa aaatgttccc tgaaaacgga gcaaaagtgc
     2041 cagaaatccg gtttcctggt tttacggaag attgggaaga gcgtaagttg ggagaattag
     2101 gtaaaactca atcgggaatt ggatttcctg atgccgagca aggtggttct gaaggtactc
     2161 cattttttaa agtttccgac atgaataaca ttggtaatga gtatgaaatg agaaatgcaa
     2221 atcattatgt atctaatgag caaattgagc gtaaaaaatg gaagccaata aaagatgtgc
     2281 cagcgattat atttgctaaa gttggcgcag caataatgct taatagaaaa cgtttggtca
     2341 caagcccttt cttaatagat aacaatacca tggcttattt atttaataat acttgggata
     2401 tctattttgg taaaatattg tttgaaacta taaatttgcc aagatactcc caagtagggg
     2461 cattgccttc ttataacagt tctgatattg agaatatcag tgttaaggtt cctgtaaagg
     2521 atgaacaaca aaaaatcggt actttcttca aacagctaga tgatacgatc gctcttcatc
     2581 aacgtaagtt agatttacta aaagaaacga aaaaaagctt cttacaaaag atgtttgtct
     2641 aggagggaag aagaatgaaa attagtcacc gtgaggaagc agaagtcgaa gaacagttga
     2701 ttcgtgtact tggagaaggg cataatcagt ggacgtatcg tccggatcta aaatcagaag
     2761 aagacctttg ggtcaattta cgccaaaaaa ttatctcaaa taatcaagca gaactgaatg
     2821 attttccttt gaccgataaa gaatttgaaa cgatcaaaac ggaattactg ctgcgaacga
     2881 aaaccccatt tgatgctgcg aaatggttaa aaggtgaaaa tgggatggct cggatcacga
     2941 ttgaacggga agattcgcaa ttgggttctg tctcacttat cctttattcc aatcaagaca
     3001 ttggtggagg aatctctact tatgaagtgg tccatcaaat tgcaaaacgt ggaagtaaca
     3061 tagaagcacg ggatcgacgt tttgacgtca ccttattaat caatggatta ccgattgtcc
     3121 aaatcgagct gaaacaagta actgcaaaag atggtgttta tcaggcattt aaccaaatca
     3181 aaaaatatgc agaagaaggt atgtttcgga acaatatctt ttcgacctta cagttgtttg
     3241 tggtttccaa tgaacaaacc actcggtatt ttgcaaatgc cttaccgaaa gacttgcacc
     3301 ctaagttttt atttagttgg cgaacgaaag acaatgaaaa agtagaaaac ttatatgagt
     3361 tttgtaaaca agtgttaaat atcccggatg ctcatcgttt gattgcggat tatacaatcg
     3421 tcagtgaaga tcaagacaat aaaaccctca tggtgttaca tccttatcaa gtacatgcga
     3481 ttcaagcctt gtttattgcc gcaaataaac atcaatcagg gtatgtgtgg catgcgactg
     3541 gttcaggaaa gacgttgact agttttgtat ctactaagct tttagcacga aaatcgggga
     3601 tcgatcgaac gatcatgtta gtggaccgca aagatttaga taatcaaacc accacagagt
     3661 ttacaaaatt tgcttcggaa tttaatactg ggatttcttc cgggaacgcc aaagcgaata
     3721 gcttgatcgt tggaacggga agtgccaaag aattaagtga aaccctgttg gcagatgcca
     3781 atgcaaatgt tgtaatcatt acaacccgtc aaaaactaga tgcggcgttg aaatatgcaa
     3841 agaaacaaga agagaaaaaa ggaaccaatc gcttccaaaa attaatgggc caacatatcg
     3901 tgtttgtagt
//
LOCUS       DARBDV010000152         3896 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000152 DARBDV010000000
VERSION     DARBDV010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.159"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            289..1104
                     /locus_tag="SCM56_002794"
     CDS             289..1104
                     /locus_tag="SCM56_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111520.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            1230..1487
                     /locus_tag="SCM56_002795"
     CDS             1230..1487
                     /locus_tag="SCM56_002795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111521.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(1739..2311)
                     /locus_tag="SCM56_002796"
     CDS             complement(1739..2311)
                     /locus_tag="SCM56_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1111522.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2327..2932)
                     /locus_tag="SCM56_002797"
     CDS             complement(2327..2932)
                     /locus_tag="SCM56_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HEG1111523.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            complement(3333..>3896)
                     /locus_tag="SCM56_002798"
     CDS             complement(3333..>3896)
                     /locus_tag="SCM56_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEG1111524.1"
                     /translation="YFKSEKDTSSLTLTLEEGVEPDKVTSKVINQLKEKGSLKHLGEY
                     EVLDTAMLTKGIGQILSTITYFITAVAGISLFIAGVGVMNMMYISVSERTKEIGIRRA
                     LGATRKSIMLQFLLEGLILTLSGGLIGYLLGMALAYSVGSLIKVNVSLDLFTILLAVG
                     VSSVIGLVFSVMPASEAAKKDLIDILR"
BASE COUNT         1294 a          715 c          582 g         1305 t
ORIGIN      
        1 aaaaataaga aattcaaaaa agaaaaaact ttccttaaaa acaaaaaata ggagtattca
       61 gtttcttaat agttttgtaa tagttgtatg tagaagtttt cttaatagtt tttgtaataa
      121 tttattaatc gtagtttata taacttattt gtaagtgact agagaattaa ataaaaaata
      181 attactatct agtacgaaat taattacata ggataatata ttaaggtgct ggactacaag
      241 taaaaaatac tgtatgatca tgacagattc aagaaaggag caataagatt ggttaagata
      301 gcaggacttt ctaaaggtgg aaaaaccgtt tcacaacttt ataaacaaaa ctacagagac
      361 tggagacagt tcagaaaaaa cattaaggat tcttacttta ttctaccaac agaacttgaa
      421 aaatatttac cgaataacaa agctattaat ttatatctgt attactgttt taaggcaaag
      481 aatgaaggcg gggattcctg gcattcggtc aataccatag cagagtctct aggagtgaca
      541 actaaaacta taaacacctg gaataaaacc ttagttgatt taggtatgat tgcacgtata
      601 gacgataacc atctcagtaa atcgacaatt ttacttcctc tctctagtta ctataaatta
      661 gcccttgata gttctttaaa ggatgttacc gagagcactg tccatgaaat tgatggcaac
      721 ttggttgcag tttatcattt gtttgaatgg agaaaaaatg aggctgatga aaattataac
      781 gttccttata acacactctg ttgtgttttt aaaagaaaaa cacaaaaaga tactatctat
      841 aaatttatct tggtccaaga tgagaaaata agtaatttcg aattaagcac tccttctagt
      901 aagatttatg acgatgcata tagatttgaa tgtcaagatc tagaagccct cctttccgca
      961 aataatctaa acaatataga acatgaaaac tttgttgtaa acactagatt taatttagtt
     1021 tctgaaaagg actcagattt gttagatttg ctgatagagc ttactaaaaa tctagataag
     1081 aaagaaaaca ttactgtact ataactagca tcggtccatc attaagtttg gactattaga
     1141 acacttgcta ttacagaaaa aggtatgcta gccacgctta ctatttattg gtatcaaaca
     1201 agtatcttaa tttgaaagga agatgattta tggctaattt atatatagag aacaaaggag
     1261 acttgaatgc cattattact ttaatcgtag ttatggtaaa atcaataggt aatatcacaa
     1321 taggaatgct gtttattata ttagcagcag tgctgatttt tatgatagtt atttggttct
     1381 taaatgaaag atctaaagac aatcaacacc gtagggatat ggagataaaa tcaatagagt
     1441 caatagatta tttatcgata ataacactaa aaaagccacg ttcctaaacg tgacttacgc
     1501 tgaacgatat gtgttgtttg gtagacgaac aaatcgttca ttataaatat atgatggtat
     1561 cctaatatag taagcgtggc tagcatacct ttaattactt taccttttac agttaccata
     1621 cacatttttt gagcagaaat caagtaaata aaaaaatatt atatttcaca acatgctaat
     1681 agactcatat ctagaacgca ttaagtattt acctgtctgg ttcgctgaga cttctttttt
     1741 aactttcttc aattttcttc tttattctaa aaagcgtact gcgactgaaa ccggttatag
     1801 cttcaacttc cttatacgat ttacctgaag taagcagttc atatgcgtgc ctcttcttcg
     1861 gtgtgattgt tgctttcgga cgtccttctc tgaaattagg attattcttt cttgcaaaca
     1921 tttttccttc ttgggtacgt tccacaatca tatcacgttc catttctgct acactcaaca
     1981 acgtccgtgt caccattcgc cccatggacg tattttcaat cgttcctaga ttcaaaacct
     2041 tgatcgttac gttatcatcg agtaaaggtt caatgatctc taatgcttct ctcgtgttac
     2101 gagcaaatcg atcaagcttc gtaacaacta tttcatctcc aggcttcaga ccagccaaca
     2161 gctgattgaa ttttggccga tcccgttttg tacccgtaaa tttttctgaa aaaatattct
     2221 tcttctctac gccgttctca agcaactgtt gaatttgttc ttttgtactt tgagaagcgg
     2281 ttgagacacg tgcatatcca tatttcgtca tttggtccac ctacttttac aaatcatcta
     2341 tcgtatactc agaataccct tcatagaaat agctgacatc gattcctttc atatataact
     2401 cgcgatcgtc aattttatca gtcagagcat tggaaattag atagcgtatt tccaaatcgt
     2461 ttatgggact tcgctccatt gcagaaagat agtctgcttt atcgattaag ttccaatcta
     2521 caactttttg caattcttcc tttaaaatga gatccagcca aattctagta cttcggccat
     2581 ttccttctcg aaatgggtga gcgatattca tctccacata tttcttgata atctcttcaa
     2641 aagaagtttg tggcatctga tcaatatgct gtaacgaatg ttctaaatac ataactggtg
     2701 caaatcgaaa atttcctttt gcaatattga ccgatcggat ttttccggca aaatcgtaaa
     2761 tatccgaaaa tagaaattca tgaatttcag ataaaccttt aaatgtacca acttcgattt
     2821 catttatttt tccacaatcg tacaattctt tcgctttttg tttactgagt ttttcttcaa
     2881 ccttagccaa ctcaacttga ttggttaatc ccaatttatt ctctaaaatc atgcgaaatc
     2941 tccttaactt tatgacataa gtttatgaaa cactctcaac cccattatat cgcgattttg
     3001 tttatgtgtc aaaaactatt aagtttctga cccccgatag atttttcaag tgtctctatt
     3061 ttgcacagtc tagttgacct ttgatacacc tctatattta tagagaatct aaaacatcaa
     3121 catggcatac tcaaaattct aaatattagg gataaagaat tctcctatac aatcaaaaat
     3181 ttcctttatt tcgaactccg gtcacctaac tctattgatc tcttacaagc caaagaatat
     3241 ctctatacta caaagaaaca atagggtgcc tggttggtct ctttgtgttt tattttagaa
     3301 tctagaacgt aaatattctt gtcattctga ttttatctaa gaatatcaat taaatctttt
     3361 tttgcagcct ctgaagcggg cattacagaa aagaccagtc caataaccga agatacccca
     3421 accgctagta aaatagtaaa tagatcaagt gacacgttta cctttatcaa agaaccaaca
     3481 ctataagcaa gagccattcc taataaatag ccaattaacc ctcctgaaag agttaaaatc
     3541 aaaccttcta aaaggaactg cagcataatc gattttcgag tagctcctag ggctcttctt
     3601 ataccgatct cttttgttcg ttcagataca gatatataca tcatgttcat tacacctacc
     3661 cctgcgataa ataaagatat acctgctaca gctgttataa aataagtgat cgtactgagg
     3721 atttgaccaa ttcctttcgt taacatagcc gtatctaaca cttcatattc ccccaaatgt
     3781 ttcaaactac ctttttcttt caattggttt atcactttag aggtcacctt gtcaggttca
     3841 actccttcct ctaaagttaa cgttagagac gatgtatcct tttcagactt aaaata
//
LOCUS       DARBDV010000153         3847 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000153 DARBDV010000000
VERSION     DARBDV010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.160"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            133..2232
                     /locus_tag="SCM56_002799"
     CDS             133..2232
                     /locus_tag="SCM56_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEG1111525.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            2428..3291
                     /locus_tag="SCM56_002800"
     CDS             2428..3291
                     /locus_tag="SCM56_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEG1111526.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            3396..>3847
                     /locus_tag="SCM56_002801"
     CDS             3396..>3847
                     /locus_tag="SCM56_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEG1111527.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEK"
BASE COUNT         1533 a          639 c          776 g          899 t
ORIGIN      
        1 ttttttttct ataggaagga aattcattca ttatttttaa gtaaatccta aaaataagct
       61 attggcgtga cgtatagaat atgatagatt aaatggtagc gtaaacctag taaaggagcc
      121 gatcgctaag tgatgaaaaa atgtccaaat tgcggatcga aaaaaatgga gcagaatact
      181 atctgtccag actgcggtca tcaaataact gcagaagatc agcaaataaa agaatcaatt
      241 gaagaaacaa atacagatgc agctatgcag gataaatcaa ctgaagatgc agtctttcct
      301 gatacagaat taaacgatcc aatcgaatgg tcggaattga aagatttacc tctagaatct
      361 gtaatggaat tattcgaaaa agcagaacca gaagacgaag aaccacctgc tcacgatcaa
      421 gtaactaata tagagaaaaa tgaacagaag aaagcaactg ttcatgagac ttcggaagaa
      481 tcaggaaaga atcaagaaga aacgcttgaa tcaaaacaaa aaagaagagt agctgaactg
      541 aaagaaatcg tcgataatga ggaagaaaat tcgatactat ccgcttatat caaagcacat
      601 cgtgaagata caaaagaaga gcacgcgaaa gagctgctca aaatgatcag tgaaaaaatt
      661 gcacaggaaa acaaagtaga gccagaaaag gaacaagaaa ctgaaaaaga ctctgataga
      721 gaaaacgcca caggtaaaga aacagtaaaa ataaaagacg taaaaacgga tatcacagaa
      781 aaaactgaag tatttactga aaaacaatta gaaaaggaaa aaacgaataa actgccggaa
      841 gcagagaaaa aagaaatact cgaaaacaca gacaaaaagt caacagagcc cgatgaaata
      901 aatagtgaat ccgatgctga aaatcaatct gtaagcaaag agggtactga tcttgaagca
      961 aaaatagaaa aagaagagaa ccagcctaag ctgtttgaag cagaagcgaa atcaataaca
     1021 atcgtgccag atactgaaaa aacacatcaa gaatcaaact tagaaaaaac agatccagat
     1081 aaagcgcaaa agaaaaaatc aaaaaaagta ccttatctcg ttttagcagc agtacttcta
     1141 ctcggtggag gaagctgggc ttattatgac catcatcaaa aagtagaagc cgaagtagca
     1201 gcagaagcaa aaagacagca acaaatggaa gaacttcatg agaaacttgc tgccttttat
     1261 acagatgatc accatcaatt tattcgtgca gatatgattc atcaagattt atcaaaacta
     1321 aaaaatgatt tatccagtct taagaatgaa aaaggatatt cagagttaga aacaacttac
     1381 caagatatcc aatctaagat ccgagatatc caaaaagtga atgagtggtt catagcacct
     1441 gccatcgtcg atgatcatct agcagatgac atcaagttga aagcagataa agagatccaa
     1501 gcaatcgaaa cggataacac agattttgga aaattgattc aacaagcaca aacagaagct
     1561 aaaaatcaat atgctgaatt gcaaacagct aaagaaaaaa cagctatagt ctttaaagac
     1621 ggaaaagtag tggattcagc aacacgcgac caatacaaga cggcaaaaga agcagtagaa
     1681 aaagtgaaaa atacagatct atctgctcag ttgaatgaac aattgaaaca agtagataag
     1741 atgctcactg ataaagagaa aaaagaagaa gaggaaaaga aaaaagcaga agaagcgaaa
     1801 aaagcagctg aagaacaagc aaaacaagcc gctcaagcag cagctgcagc tcaagccgct
     1861 caacaagctg cggtcgcaca agcagcacag caagaagcgg catcctcgac cccttcctct
     1921 tcaaactcag cgaatcagcc aatcaaatct gccagagcaa gtgatattgc cgatgcatct
     1981 aatccagcat ggacgtgggc accaggtgtc caaagcaaag tgattgctac atgtatccaa
     2041 cgtggatata tcgttgaagg aggttacaga ttagaaaaag ccagaatcga aaatggggaa
     2101 ggctattata atctatatgc cacttcaaca aaatctgcat tgatgaaagg cattggagaa
     2161 agtgctttgc cattttacat cgtaaccatc aactgtaaaa ctggatggtt cggaggaaat
     2221 gggagcaatt aattttaggc aaatgaaaga ggctgtgagc ctgccgttta acaccgttta
     2281 ttcgaaaaag gggagtatga cacaactgtt gtcatactcc ttttttcacc tttccaaacc
     2341 aaggctcagc tattcaagta gataacatta tgttatgata gcaaagaaat gtattaagaa
     2401 aacctttcac tatagaggga gaaaaaaatg aacgatttat tagcaactat atttacaggt
     2461 attattattg atgaaaatga aaaaaactac tttgtccaaa aaaacggaca aacctttggc
     2521 ttaagcaaat cagaaggtga acacaaaatc ggtgaagcag ttgaaggatt cggttaccaa
     2581 aatcaaaaac atgaaaatcg gatcactaca caaattccta aaagccgtat cggtcattac
     2641 gcttttggaa tcgttactgc tacacgaaaa gatttaggtg cgtttgtcga tatcggtctt
     2701 ccagataaag actttgtcgt atctcttgat gaattgccga caatgacaga gctatggcct
     2761 aaaaaagggg atcgcttgat gatttccctt agagttgatg caaaagaccg tatttggggc
     2821 agtttagcag atgaaaaaat attcaaatct ttaagcaaat atggaacaga agaaatgaaa
     2881 aacaaagatg tgataggaac agcataccgc ttgaaattaa caggtacgta cgttttaacg
     2941 gaagatttct atcttgggtt tatccatcct tctgaacgat ataatgaacc tcgtctaggc
     3001 gagcagataa aaggtcgagt gattggtgtc cgaccagacg gtgtactgaa tctttcattg
     3061 aagccaagag catatgaagc tatatcagat gatgcacaaa tgattttaac cattttagaa
     3121 cgttctgcag atcagcaaat caactttaca gataaatcgg atccagaaga aatcatgaaa
     3181 gcttttggta tcagcaaagg agcatttaag cgcgcattag gtaatctttt gaaacaagga
     3241 ttgattacac aagaaaacgg tgtgacaaaa ctagtcaaaa aatctaattg agaatccctt
     3301 gcatgttttt cttaactagc ttataataat tttaattaac atagtgttat atcttttttt
     3361 atttgtaatt attataaatt gttggggttg agtaaatggt catgaatgct atttcggcga
     3421 tgaaaaaaac aaaacagcag ctacatgaag cggggtttaa gctgacaccg caaagagaag
     3481 ctactctgct tgtgcttttg gaaaatgaga aagagcattt atctgcagaa gaaatcttct
     3541 ttttagtgaa aaaaaagagt ccggaaattg gacttgctac tgtttatcga acccttgaaa
     3601 tcttgacaga attgaaaatt gtcgataaag taagttttaa tgatggatta gcccgttatg
     3661 atttgagaaa agaaggcgcc aaacatttcc atcatcattt actatgcttg aactgcggca
     3721 cgatcgagga agtagaagag gatcttttac ttgaagtgga gcaagtagta gaagaacgtt
     3781 atcatttttt agtaaaagat catcggctaa cgttccatgg aatctgtcag gaatgctatg
     3841 aaaaaga
//
LOCUS       DARBDV010000154         3845 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000154 DARBDV010000000
VERSION     DARBDV010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.161"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(659..1216)
                     /locus_tag="SCM56_002802"
     CDS             complement(659..1216)
                     /locus_tag="SCM56_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111528.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1395..2006)
                     /locus_tag="SCM56_002803"
     CDS             complement(1395..2006)
                     /locus_tag="SCM56_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111529.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2119..2376)
                     /locus_tag="SCM56_002804"
     CDS             complement(2119..2376)
                     /locus_tag="SCM56_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111530.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2973..3251)
                     /locus_tag="SCM56_002805"
     CDS             complement(2973..3251)
                     /locus_tag="SCM56_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111531.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3285..3536)
                     /locus_tag="SCM56_002806"
     CDS             complement(3285..3536)
                     /locus_tag="SCM56_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111532.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1153 a          794 c          523 g         1375 t
ORIGIN      
        1 tctcacgctc taaacacgta taaacggcgg aagcagaagc aactccttct tatttcttgg
       61 agttaaacgc ttctgtccca gcctccttat tccgaataaa tggtgtccaa aagcttgcat
      121 ctgccagatt atctcatttc ttatgacaag ctaagcaaat tatctccacc aaataattta
      181 ttcatatttt tgacttgcgc atactaacta attcttaaat tttcatacaa aatttctcaa
      241 tttcccttaa cgagtaatat ttatgtacgg tgattctcac tagtatccca atcacttttt
      301 aagttatccc gatttcaatt ctacttcaaa aatagttgct tttatgtatg ctgttagttg
      361 attagatggt catttgttga gctaggtcgt ttattttaaa caagttgttt ctttagctta
      421 tacctatctc gtctaccaaa cgcaagatgg caaacacaaa tgcatacgca tctgtatttg
      481 ccactttgat tcgtataaag ctaacaacta ttaactcctc tcagctaata gttgctctct
      541 tttttattca gattcatttt tgctgatcac agcctagtat ttcaatggtt ttgaacacta
      601 aaccaaattg ttccattaca acattcactg gctgatgaaa tggattagta gatttcgatt
      661 attcaaagcg attttctatt ggtggatcgt gttcccaact aataagacgg attccaaacc
      721 tatcgtacag atcttctaaa ccacttataa attttgcacc tatttcaggt gttttattgc
      781 gtggatattc tattgttacg ccattaaatg gataccaatt aggattaggc tctctttttt
      841 tctgtacatc tcccatagaa tagttagcac tttctgggac ataatatatt cttcgaccat
      901 cctctaattc atctaataag atatttcgtg atcgtctatc acttgctaaa tgtagacgcc
      961 acgtgcgata ccatgccatg ctacctgcat ctgcagaaaa tccatcaaat aatgcaacaa
     1021 ttgtatcttc tatcacaatc ctttccccat ctacttcata ttctaatcta aaatcaaact
     1081 cccctcgggt aatttctggt cttggtggct taggacccaa tattattcta cttaaaaccg
     1141 cattcgttcc cagatgaaac acagctataa caacaataat gattaatatt ttgtttctat
     1201 ttgataaatt tctcattatt cgcacatcac ctctacattc tcacatatta gttcgtaata
     1261 ctcctttaat atcaaaatat ctactggtcg taataatatt cgctatgaat tcctactact
     1321 cgtataagaa aggccaaatc atcagctttt acttgctgat gatttggctt gtttctattt
     1381 atcgtaattg ttttctaatc accaaaattg ttaacaatcg gttcagatat ttcccagttg
     1441 attaaggtaa ttccgtattg tgtcaaaact tcttgcgctt ctccgaaatt aagtacagtt
     1501 cttatgtatc cactttctcc taatataaag tctacctctc ttttaaaacc ttcctcgaga
     1561 tttctaaggg ctatatgtgg ttttaatgac attgattgta cattttcccc catataatat
     1621 tgcgcacttc ctcgactaaa atagattcct agatcgtccc caaaaggtag atcttctcta
     1681 ccactagcta atctacttct ccatcttcgt tctcttttca tagaacctgc gctgaactca
     1741 cacccgttaa actcagctat gacagtatct tcaattatgt taatctgtcc atctagttcg
     1801 tattcaatcc taaatggaaa ttctccatag gtgatttccg gttcgggtat tttaggtgag
     1861 attaatgcaa agaaaggaaa tattaagctt ggcaacatca aaaaaactcc tcctatgatc
     1921 acaattcccg atgtaatcca aaatataatt tgtacaattc tcattgatcc attgtattcc
     1981 attttattcc ccctattact attcatatcc gcctatctat atcgatcttc caatgcagag
     2041 ataattgctt ccataccttg attttttact gagttaccta tgcgagatat cattctccac
     2101 taaggtgcat catattgtct atctaacatg attaattctc caataggtgt atactcctcc
     2161 aaaaacaaca tttaatgcat ctccctcgac aacataagct accatgtttg ctgtatatgt
     2221 gtttgagtct aattcataag cagataaatt tactgtcgct ggattaaata taatcgcatt
     2281 cgtatttata gctactgaat ttgctgcttc agcaccacct tttgaatgtc cgatcattgt
     2341 gatttcaata gtaggattta cgtggacagc cctcaccgtt taccctgacc attatagaaa
     2401 tcgcttactt ttttgaacta cgcaacaaag gatacaatgc tcctaacttc actgtctcca
     2461 tttttgtcca ctaaggaata taaaaaatag gcattaattc atttaattcg gtcacaacac
     2521 gttacctttt aaatcattat actgtatctc atacattatt atcaataaac tattataaaa
     2581 atgcaaaaaa tgctttatca atctattttg taattggtca taatatcatt gaaatcatac
     2641 cctgtatcga tttttcctat gaatctcgtc tatttgtgat tgacgaaaaa gaggcaagac
     2701 agacactgga atggtaattt tacccactat tttgctccaa ctatttgtaa tcgtacccac
     2761 ttttgaaata ccgagatgat cattttaatt gatatgctga ttcccaagca aaaccccaag
     2821 aaattaaatg ctctatcgca tctagcttct tgggttttgc ttatataaat tccaacattt
     2881 aaatttttta tgctgttcgg tctcagaaat gccgtttttg cttatatcac aatatcaaga
     2941 tgtcaaatta ttcgtgttgc cgatgacttt gattaatttt tttcaaattg tttattaaga
     3001 ctaatgctgt ctttttgcag ataccaatgt tcgaactttt taacattatc atatgatcct
     3061 gcaagtttta acttcttacc attagatgtt atcaaagtta taattaacct tgcctttttc
     3121 ccattaggat cttttatttt atgaattttc tgcaggtaat ctctcctaat ggcagctatt
     3181 tttacctcat cagtgctgat aaaccattga tcatccacat acatactgcc tgttatgttg
     3241 tgtttttcca tttcttccga gaacgaaaag tttaaggctc gttcttactt ccaaagttga
     3301 atgagtccat ggattggact tagtgtgaga attgagaaca acaataagaa tccatatcct
     3361 cccgcaaaca tccctaaggc tatactgtct ctccatccat aagtaggatt agaagagaaa
     3421 agagtcaccg tttgtaataa tatgaaaagg aataatccag cacaaacacc aaagataaca
     3481 actccaaaca ttgaattttt atacaatgac catttattta taggcttttc tttcatttta
     3541 cttcccccta ctgttctgtt cacacggaat gaattcaaag ttcataagcc ctcctctttg
     3601 aacatatgaa tacgacttgc taactatact ttaaaaaagc ttgttttaag cggttatagg
     3661 cgagttgtaa tctctattca tctcattagc aaataacttg ccgaaaagtc tactgaaatt
     3721 aaagcagata gtggaagaat tatttagcca ggatagacac tatttgtgaa acacatgtct
     3781 ttacttttga aattctatga tgaaaagcct tggaatacag tagctcttaa ttggtaatac
     3841 ttttt
//
LOCUS       DARBDV010000155         3824 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000155 DARBDV010000000
VERSION     DARBDV010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.162"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            574..1614
                     /locus_tag="SCM56_002807"
     CDS             574..1614
                     /locus_tag="SCM56_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEG1111533.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            2438..2770
                     /locus_tag="SCM56_002808"
     CDS             2438..2770
                     /locus_tag="SCM56_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HEG1111534.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            3389..3730
                     /locus_tag="SCM56_002809"
     CDS             3389..3730
                     /locus_tag="SCM56_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HEG1111535.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1410 a          494 c          698 g         1222 t
ORIGIN      
        1 tattaatggt agtatctaaa ttaacaggta gtatgtaact actttaaagt aatttttttc
       61 agattaacac agtagtatct tattaaataa gttaatttta atggtagtac ataattatta
      121 tatatatctg acattggtag tacttattgt attttttagt agtatataat ctattgtttt
      181 ctagcctaaa gccttgtctt ataagaataa tataatatat actactgaaa taaggtagta
      241 tatattacta gtaatggtag tatgttttat atgcataagg tagttcttaa tttatttttt
      301 gttttgacac tggtagtact taattatctt taattttaaa actaaatttt attcatacgt
      361 ttttctggta gtatttaatt ataaataata tttatttaaa gtagttctta atgactttaa
      421 aggtagtaca taattcattt tatttctgat tttaggtagt atttaattaa aaaacagttt
      481 taatgcgttt gcatttttta tttatctttg ctattttcga aaagagtagg taattttaca
      541 taaataaaac aacgtgaaag tagaggatct acaatgactg attttaaatt ttttaaggcg
      601 gatagagttt acaacgaatt attttatcaa tttccaaaag tctttattgt ttctgacgaa
      661 tacaaaaaaa tgaaagattc aactaagatt gcctatatgc ttttaaaagc aagattagag
      721 atcgcaatca gcaaacggca aatcgatgaa gaaggtaatg tttattttac ttatacgaca
      781 aatgaactat gtagagtatt aaactgccaa aaacaaaaag cgatagcaat caaaaaagag
      841 ttggaatcct ttggtttatt attacaaaag cagatgggat ttaacaaaca gttagggaaa
      901 aataatccta atagactata tctagcagaa ttaaaagtct cagaaaatga tatctactta
      961 ctcgaaaaat ttgatagaga gaatagggaa aacgttgata aatcagaggg tatgaaaatc
     1021 atacccaccc tcgacgaaaa atcagacgct gaatcccttg gggctcaaga gggtatgaaa
     1081 atcataccgt gccaaaacgt tgataaatca gagggtatga aaatcatacc agaacttaat
     1141 aataatatat tagacactaa tagacacaat atagacactg aaaaagaccg cctacaagat
     1201 caattgttgt tagacaattt tgagacaatt atgacaaacg acagcattgc tacgtttgtc
     1261 cctgaacgag tattaaattt gataaaaaca ttttcttcaa gttacagtga agctcaaaaa
     1321 accgtccaga ctattcataa tgcaaagaaa aaagctgaaa tagaaagtgg tatttcgata
     1381 gtttttgaag aactcgatag ttattatgtc aatgcagaac aagaattata cacgacactg
     1441 ttaaaagcct atcaaaaatt aaaaaccgaa aaagtcgaaa atatccagaa cctgattttt
     1501 gtctatgtaa aaaattggtt tatcgaaaaa ccaatagctg ctaaagtatc aagtgaaaaa
     1561 cgtttgaatt atgaaagctc cccaagcact attacgaaag actggttaga gtgaaacttt
     1621 actgacaaag tgattgttgc ttcatggagt aacattaaaa ttaaaaattt tttattattc
     1681 taattatatt acgaatatta caaaataata gctattttaa tagaatttat aagttaaaat
     1741 attatgataa aaatcacaaa cgtagccttt tacgtcataa gtgttaaaag aaataattaa
     1801 tttcaaatat agaaaaggga acgtatgttt gcatactcct tttttttgag ttataataat
     1861 acgcgaagag agataatttc attatttaaa caagctaaaa atcttgatat tacttctttt
     1921 caggcaatac gaatattatt cgcttacatt ttttgtataa atttaattgt ctgctatgct
     1981 tgtgatgaat tgattttcgt tctgcacttt tggatgataa atttggacaa ttgaaaatac
     2041 aaaaaaaaga aaggcggtat gaactcggaa ggctggtaac cttcgtcagc gttcatacac
     2101 ggcattaaga tttggccctt aatgcgttaa cctttcagtg ttacggtatt acccgcaatg
     2161 gatttagtat aacagatatt caaaagtgat accacttttt ttggctgtgt taaggttaaa
     2221 aatacgtgat attacgatga atctatgcct aagcacaatg ttataaaaat gtccgtgtgg
     2281 ctctttgccg tatgacgaac gctgaaatta gagggaacgc atgtttggat gcgttccctc
     2341 ttttttgttt gtcatgaacg aaaatcaatt ctctattttt gaaaaataac aagaaaaact
     2401 tgttaaaaat atttaaagaa attgggtggt ttattttatg tcatatacag gaattttgag
     2461 tctcaaagat atttgtcact acggtaaacg gtgtaccgct acagagaaaa tcacgaagaa
     2521 actatcaact ggccaaagta aaaccgttgt gcagtgtaag aaatatataa tccagaaaga
     2581 caaagtctca gaggaaatga tttattacat tgggaaacaa aagcaaataa ttttaaaaga
     2641 tcctatcccc ttaaaagagc tatatccaac aatcaaacat gtttatgacc aaaacggtgt
     2701 actgattggt cgaagaaaaa atggagtctt gcgttgtacc gcaaaaggga tggggcgttt
     2761 gatcggctga attgatcatt ctcataactt gtattttatc gtttttcgct tgcttgaaaa
     2821 tattgtcgag acttctaaag gaacttagag tcactagaaa aataaggagt agacttgatc
     2881 tatcaacaat tcccttcggg gagtgacggg gtaggtttta acgattggta acgtaaaaaa
     2941 ttggtcaatc gagtaaatgc gcttgtttgg atagtggtga aatgaatttg tgtaaataat
     3001 aagcgcagag taaacaagag ggaatttaga agttagacaa gttagaatag cgtctagatt
     3061 aatcatgagc agctactaag ggatctagac tttgacgcaa actcatggaa taaggagtag
     3121 gtcttctcgt aatggggagg acgcacaaga aattgtgtcg tgtgctgcag tacatgtaga
     3181 tacttgcgag gacgttgatt attggatcaa tcgagcattt accgctgtct actagggttt
     3241 ctcgtttcgg acgccataaa cgagtcgaaa ttttttatgt atttttaagt aaacgaaaaa
     3301 cgataaaata tgaacaagtc ctgaataaag tgaaaaaatt gatataaaac atagagaaaa
     3361 tgaaatttac gtaatggagg aatgacaaat gaaaaattat gatccgaata tacgattggg
     3421 cacacatact attaaggttt ctttccaacg ttgggattac aaaggatttg tgacgtttag
     3481 aagaggcgga aattgtaaag gattagatgt tttagcatta gacgaggatg atttatacga
     3541 tcaaacattg actgataatc caattggatt cggcttgcta cctgaagatg atgaaggtaa
     3601 tgaatggttt aaaatgacgc ttatgaatga taatggagac gaattatctg ttgaagatac
     3661 ttggtcatat ttgagtgatt atattgtcag ctttgaaatc attgaatttg tagcggataa
     3721 agaagaataa aaactggagg aataaatttt acagataaag gcttagaagg agtcacgatg
     3781 attaataaaa aggtagtatt accttaggca ttgagtaagg aatt
//
LOCUS       DARBDV010000156         3766 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000156 DARBDV010000000
VERSION     DARBDV010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.163"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(685..978)
                     /locus_tag="SCM56_002810"
     CDS             complement(685..978)
                     /locus_tag="SCM56_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HEG1111536.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(1381..2559)
                     /locus_tag="SCM56_002811"
     CDS             complement(1381..2559)
                     /locus_tag="SCM56_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1111537.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(2663..2977)
                     /locus_tag="SCM56_002812"
     CDS             complement(2663..2977)
                     /locus_tag="SCM56_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1111538.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            3084..3302
                     /locus_tag="SCM56_002813"
     CDS             3084..3302
                     /locus_tag="SCM56_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111539.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWKS"
     gene            complement(3297..3692)
                     /locus_tag="SCM56_002814"
                     /pseudo
     CDS             complement(3297..3692)
                     /locus_tag="SCM56_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT         1205 a          721 c          553 g         1287 t
ORIGIN      
        1 aataaaaaat actctttttt tacgattatt gaaaaaaaaa gataaaactt gattaattga
       61 agtattgctt aaatttggaa gctatgaggg aagaataaaa gagttaggac gggattatct
      121 actatttttc tttgttttaa atatttgtta tctttgaaca taaaagagaa atgtgtcgac
      181 tgcagtgttg tgtccctcat cacgaataaa cggtgttcaa ttagctgatc ttgtattcac
      241 ttaaaaaatg gtaaagtacg agttgctatt ccccattttc aattttctaa cgctcaggac
      301 aaaatactgt tttcacaacc tcattggaat aaaagcatat atattgtttc aagaacgaaa
      361 tcttgttatc tttttaaagg gcatctgtcc aaaatctaat tttaggaggt acgttttggt
      421 atttattcgt acaaataaaa gagaattatc tttattcatg taacgcatcc aattgatttt
      481 ttcatgtacg ctcctggaac gattttactg acgattgctt atttaactgc gaatcgctca
      541 ttagctttta tcatagctat ccatattttg aataacgttc ttggctttgt attatagata
      601 cagatgacaa aaaaagttca agtatgcggt gtagaaccaa tatcgcatac ttgaactttt
      661 ttcaatgagt ataaattaaa acttctaatt ttcaattccc atcatctgat atgcccgacc
      721 gtctgaactt cttttgaaaa agccaaatgt gattagataa cgtctttgtt cgacataatc
      781 gccactagaa aatacgttca gcagtatcga attaacctct gtttctgtat aagtcactcc
      841 atggtcaaag cgtttcaaga cttctttcaa taggatgtat ttcttttttg ttgaacttgg
      901 tacttgtttt acttttccat cattcatgaa tgtgtctaaa actttcatct tttcttgctc
      961 cgtataagta taaaccatct tctttctcct tttgattcta agaatactcc atttatgtta
     1021 gaatattctg gattttttat taaagcggtt actcttaaga aagatattat catagtgctc
     1081 ttttttttac tactacttat aggtagattt attctaatcc attattctcc cctaccaata
     1141 attaaagaaa aaactggatc agggtgttca aattacatgc taagaaaaaa gaacccttga
     1201 ataccaaggg ttctttctca gtctgataac aatgattaac gacggatttc tttgatactt
     1261 gtgaaaagtc gttaaagtat tgatataaaa ggatttttat tacctctgac tacatattga
     1321 ctaatttgtt aaaaaatcgt ttttcaagaa tcctgtaata ccagtgcttt caccgttttt
     1381 ttaactctct aaagaactaa aagcaatcag aggttttacc ttttcatttg acgttaagtg
     1441 gcgataaatt ttcgaagttg tttggtcagt atggcctaag cgatctttca agacttccca
     1501 gctacagtgt tccgctacta agcttgccat tgtatgacga gctaaatgag tatgaaatgg
     1561 cttattaatt cctgagcgat tcacgcaccg tttgacgtga tcatttacat actctcgacg
     1621 ataaggaaca ccacgtttac tagaatcaag atacacaaac aaatatgttt cttctataat
     1681 aaacctgtaa tgttgcctca tctcacccaa agccttaatc aactgttgta gcttttccat
     1741 aactagagga gttactagaa ctcgtctagc agaactaact gtcttagtag tttcaattcg
     1801 aaaatcctta ctcttacctt taactgccgt ttgcttatag aacagaattt catttctttt
     1861 gaaatctata tctccaggct gtagagcaag cgcctcgctg actcggcagc cagtgtaaaa
     1921 tagaacatgg aaaagtacag acatcggtaa ttcctcatga acaatgccat aattcaaaaa
     1981 agttctcaat tcatcgacag taagaaactt gtcctcaata ttatttatgg cttcttttaa
     2041 atcagttacc gttaactgat attttggcat tctcacagac ttcattggat tttcacttat
     2101 caaattcttt ctttgacaat atttaaaaat catatttaga acttgaataa gtgattgtaa
     2161 actatttttg gcatagccaa catttgttcc attaacgcca cattccttta aggtaaaaag
     2221 caattcttga aggcactctg gtgtcaaatc agacaacttt ttatcaccaa tccatcgaac
     2281 aataattttt aattgttcct ctcgcttatc ataggttatt tccttttgtt catttaacga
     2341 acggtaatat tggaaccatt gctcacctac aacactaaac ttgtcattgc ttgcaacaat
     2401 ttttttctta agcctttgtt gtttctccat ttccagataa gcgagttgcg cctcacgttt
     2461 tgaattaaag ccacgtcttg ttgttcgata tgcttgaaag taccatgtga tcctaccagt
     2521 agaactgatt tgcttcttga atcgatctgt tttttccatt tattgattcc tcctattttg
     2581 aaggaaccca acaagcagaa accctttgga aatctttcta tctatgtcat cattgtaccg
     2641 catcttagaa agaaaatgta acttataaag atcgaagtag tcgcaatttt tctcgatagt
     2701 cattcgaatc actctttctc atccattcta aaacatcatc acgaaaatag tgtcctcgaa
     2761 tcccgtacca acagggagga aactcaccaa gttttactaa acggttcagt gtactatcac
     2821 ttatctgaaa tagtcgcttt aaatcagtat ttgttaatac cagaggtaaa tgactgactg
     2881 caagcaattt ttccgtgtaa tcttccagtt ttttttgtaa aattgaatag aactctattt
     2941 ccagagaatt aacatttgtt ttaacttcca actccatcac ccttctctct caaaatcaaa
     3001 acgaccacca cggtacccct taaaattgta aaaaaacact ccagtactcc atcaaaacgg
     3061 actagaaaag aggaattttt aatatgaaac aaggaattgc agacataaaa ataattaaag
     3121 aaattcttga aaaatctacg gctaatgcaa tagcattcgg aacaggaatc aacttgagta
     3181 cagtaaaaaa acttaaaagt ggagagagag ctgaagaaaa attaaatctt gccgatgcta
     3241 ttaaaatcac agaatttggc atgaaaaata tgccaaccaa aatcgaaata tggaaatcct
     3301 agctcttcca aagccatttg tcgttggtac caaacactgc cgagactttt cccaataata
     3361 tcgccaattt cacgatcatt aagaccgatg aagaaggaca taaaaatgat ttcttgtctt
     3421 tgactaggta atgctgcaat cgcatttgcc aactcctcat tggaaataaa gattttcata
     3481 ccaaggacaa taaaagtacc actactattc aaatttggtt ccaaataata gaactctgta
     3541 cctaaccctt tttgttccaa tatttccatt gatatttgcc gttggcgaaa tctagcgtac
     3601 ttcttttgaa tatttaacgc ctcattgcag actactttct tacaaaaact gtcaaacgta
     3661 tgttgaatgt gggtttcctc tgagtcgccc ataatattct ccccctcaaa aaattcattc
     3721 aactcgtttt acttctatcc ctttccatta ttactacaat ctagga
//
LOCUS       DARBDV010000157         3712 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000157 DARBDV010000000
VERSION     DARBDV010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.164"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="SCM56_002815"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="SCM56_002815"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEG1111540.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="SCM56_002816"
     CDS             complement(2260..2511)
                     /locus_tag="SCM56_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEG1111541.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="SCM56_002817"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="SCM56_002817"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEG1111542.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="SCM56_002818"
     CDS             3461..3679
                     /locus_tag="SCM56_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111543.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1025 a          796 c          688 g         1203 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca ag
//
LOCUS       DARBDV010000158         3709 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000158 DARBDV010000000
VERSION     DARBDV010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.165"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            72..1118
                     /locus_tag="SCM56_002819"
     CDS             72..1118
                     /locus_tag="SCM56_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEG1111544.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            1135..2358
                     /locus_tag="SCM56_002820"
     CDS             1135..2358
                     /locus_tag="SCM56_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEG1111545.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            2447..3334
                     /locus_tag="SCM56_002821"
     CDS             2447..3334
                     /locus_tag="SCM56_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEG1111546.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            3420..3650
                     /locus_tag="SCM56_002822"
     CDS             3420..3650
                     /locus_tag="SCM56_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEG1111547.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
BASE COUNT         1301 a          613 c          784 g         1011 t
ORIGIN      
        1 aaaaataggg cttaagtttg agtgacagtt tgtaggctat gttatactat ataagtgaag
       61 ggggacgtaa ggtgaaggta ttattatatt ttgaaagtga aaagatgctt gcaaaatctg
      121 gaatcgggcg tgcgcttgat catcaaaaac gtgctctgac ggaagtcggt atctcttata
      181 cactagatga aaaagaagat tatgacatcc tgcacatcaa tacatacgga atcaatagtc
      241 ataatatggt caataaagcc agaagagaag gaaaaaaagt tgtctatcat gcgcattcta
      301 cagaggaaga tttccgaaat tcctttatcg gatcgaacca gctttctcct atcgtaaaaa
      361 aatatctggt aggattgtac caaaaagcgg actatctgat cacgccaaca ccttattcaa
      421 aacagttgtt ggaaagctat ggtatccgtg tgccgattca agctatttcc aatgggatcg
      481 acttagagaa atatcaccct gatccaatga aagaacaaaa gttcagagaa tatttcaaac
      541 tgtctccgga tcaaaaagta atcatttgtg tcggtttgtt tttcgaacgt aaaggaatca
      601 ttgatttcgt agaaattgct aagaagatgc ccgaatacac atttatttgg tttggtcatg
      661 ttcccatgta ctcgattcct cggaacatcc ggaaaattgt aaaagaagat catcctgaaa
      721 atgtcctgtt tcctggttac attcgtggag agatcataga aggtgcgtat tcaggtgcag
      781 atctattctt tttcccttct tatgaagaga cagaagggat cgttgtttta gaagctttag
      841 caagcaaaca aaacgtactt gttcgagata ttccagtgta taatggctgg ttagaagata
      901 gcaagaactg ctatatgggg aagacaaacg aagaatttat ccgactgatc caacagatta
      961 ccaatcaaga actaccgagt acgacacaag ccggttatca aacagcaaaa gagcgaagta
     1021 tcaataaaat cggtgaagaa cttaaaaatg tatacgaaag tgtactgaat gaaaaagtct
     1081 ctagcaaatt aaaaaaagta aatcaggtca aggactagtt tggaggcgaa aaacatgaaa
     1141 atcggctttt ttaccgatac ctactttccg caagtcagcg gagttgctac atcgatcaaa
     1201 acattaaaac aagaattaga aagacatggg catgaagtct atatttttac aacgaccgat
     1261 cctaatgcgg ataagttgga aaaagatgtg attcgaatgc caagtgtacc atttatttct
     1321 ttcaaggatc gaagagttgt tgttagaggc atgtggtatg cttatttggt tgccaaagaa
     1381 ctagaactag atcttattca tacccataca gaattcggag cagggatact tgggaagatg
     1441 gtagctaaga agttgaaaat acctctgatc catacttacc atacgatgta tgaagactac
     1501 cttcattata tcggtaaagg aaaagtgatc aggcaatccc atgtaaaata cttgtctaga
     1561 ttatttgcaa atcatacgac aggtgtagtt tgtccaagtg aacgtgttat tgatacatta
     1621 cgcagctatg gtgtaatagc accgcttcgc gtgattccta ctggtatcga cattgaaaaa
     1681 ttcaaacgtt ctgatatcac tcagcaggat atcagcaaat taagggagtc actaggtctt
     1741 caggaaaacc atttgatgat tctttcttta agcaggattt cttatgagaa aaatatccaa
     1801 gccttgatta atgggtttcc tcaggtcatc tccgcttatc ctaacgccag attagttatc
     1861 gtaggaaaag gaccttatgt agaagagtta gaggaattga tctcagaact gcagttagaa
     1921 gaatttgtgc agttcacagg agaagtggac aacaatgatg tagcacttta ttataaagcg
     1981 gcagattatt ttgtcagtgc ttctacttca gaaacacaag ggcttactta tacagaagcg
     2041 atggcagccg gaacaccttg tgtcgtggaa ggaaatgctt atttgaaccg attgttcgat
     2101 catccttcat tagggaaaac ttttgaaacc gacgaagact ttgcaccagc acttattgat
     2161 tatatttcca gtgaagttcc taaagatcca aaagttttag aagctaaact ttatgatatt
     2221 tcatctacac atttcggcga agctatgatc gaattttatc aagacatggt tgtttatcat
     2281 gaagaagaga tgcgaagaaa agaagaagaa gactcgattg aacgaatcaa aataaaatta
     2341 aattcattta aaagataaaa ttcaaacatt ttttatgaaa aaatccttgt ttctagcgta
     2401 tactagcata gaagcaagga tttttgattt ttaggaggtc gaaaaaatga aacttggttt
     2461 tatcggaaca ggcgtcatgg gaagtgctgt cgcacgccat ctactagaag caggacacga
     2521 agtagctgtc tataatcgga caaaagcaaa agctgatcct ttagtaacag aaggagctat
     2581 ctgggcagat acaccaaaag cggtagcaga acaaagtaat attctcttta caatggtagg
     2641 ttaccccaaa gatgtagaag aaatctacta tggacagtcg gggatttttt ctgcagatat
     2701 ctcaggacat attttagttg atttgactac aagtacacca agcctggcag aaaaaattgc
     2761 aaaaacagca aaagaaaaag gcgcagatgc gttagatgca ccggtatccg gtggtgactt
     2821 aggagcaaag aatggtacac tgacgatcat ggtcggtgga gaagaagccg tttatgatca
     2881 agtactgcct ttattcaaag aatttggtac gacatttaca ttgcatggtt ctgctgggaa
     2941 aggtcagcat acaaaaatgg ctaatcagat catgattgca ggtacgatga caggaatgac
     3001 ggaaatgctt gtctatgcac aaaaaaacgg acttgattta aagaaagtga ttgagacgct
     3061 ttcaggtgga agtgcagcta actggtcttt aagcaattat tctcctcgta ttttaaaaga
     3121 agattacaca cctggttttt ttgtcaaaca ttttatcaaa gatttaaaga ttgcattaga
     3181 agaagcagaa aaaatggatc ttgtattgcc agcaactacc caagcattga aactatatga
     3241 agagttggca gataaaggtt ttgagaatga cggaacccaa gctttgatca aactttggtg
     3301 gcctgaagga aaaataccag aaaaaaaatc ataaaaaacg agttttttta gagaaaatca
     3361 atgcaagtag gctatgcttt tgatacaata taacagaaat aagaaaagga ggacctccga
     3421 tgacacattc acaattagtc gcaatcataa aacgactgga ggctatggtc gagtcagcag
     3481 ataatgaact gcaagttcgc cgttttgaaa aagaaggcgt agagaagtgt acagtaactt
     3541 atgataaagc gaccgataca tttgaattaa ctgaaacaga tactcatcag caatacgaat
     3601 ttgataatat cgatatcgta gcgatggaaa tctatgactt aatccaataa attttaggat
     3661 aattgacatt tgaagttgaa gatgcctaaa agaagccgag aagcaacta
//
LOCUS       DARBDV010000159         3684 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000159 DARBDV010000000
VERSION     DARBDV010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.166"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..2232)
                     /locus_tag="SCM56_002823"
     CDS             complement(<1..2232)
                     /locus_tag="SCM56_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111548.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="SCM56_002824"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="SCM56_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT         1068 a          796 c          566 g         1254 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg aatccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacact tttataaaac gacgaaaaat aaatcactac cagtcaactt ctataatatt
     3481 accaaataac aaactaacta tccatcaata acgtgtttta ttttttaaaa tttctataaa
     3541 aaaacaatca ttcttaaaaa gaatatataa aaataacaat aacctttgtg atttttcttg
     3601 tttgaagaat gttagaagaa aaatctctaa atacttataa ttattttata agcaaaaaaa
     3661 tgctatcggt agtatcaccg atag
//
LOCUS       DARBDV010000160         3677 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000160 DARBDV010000000
VERSION     DARBDV010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.167"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            634..>3677
                     /locus_tag="SCM56_002825"
     CDS             634..>3677
                     /locus_tag="SCM56_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297336.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEG1111549.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVSLPE
                     DKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDIIENPGENIPAGYHKVT
                     FTAGEGTSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPITADDTEF
                     VSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVS
                     AAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT         1368 a          563 c          743 g         1003 t
ORIGIN      
        1 tgagtgaaag caattaatta ttcatgtaga gtactagaca ctttaaatat ggacaattca
       61 attataaaga aaaagaatca ataatttaat gaggctgtga caaatgtcat tttttgtttc
      121 gctaaaaacg agtgaaaaaa tgttgataac tccaatcatt cgtttaagat tctaggaatc
      181 attcagaaat gctgtgaaac cgatacttta taattataaa gaaggtgatt tcaaattccg
      241 aagaaggaat ttgaagtcac ctttttactt atgtcacagt ctcattcagt aatgcgggat
      301 aaggctcctt attccgttat ttgaatactc tgaagtttaa tctaagaaag ggtaatattt
      361 tgaaaacatg cttcatcgca tagaaatgaa cttaatttca gaaagaatac atacatatct
      421 ttttatatgt tttcagctgc tgaatggtta aaaagcacaa atatccgaaa aaaacatgaa
      481 gaaatgtact ttttttgttg taatgttcat gaaattctct aagtaaatta gttaacataa
      541 ggttagtaag attcctattc tgtgaataat acgtatcaga tttatctttt attcttacta
      601 ggaaagtaat tattaaggga gttgatttga ataatggtta gcaagaataa taagagagta
      661 tttctagaaa aaacaaagaa acgagtattg aagtacagta tcaaaaaatt aagtgttggg
      721 gttgcatctg ttttgattgg tgttggtctt gtcttgggaa caactgaact agttaaagca
      781 caagatgaaa tttctccaag cagcccactt gaaacagcga taagctccgt acaagtaggt
      841 gacaaagtag cttcggggaa tacttttcag gagaatcctg gttatacgaa aaattataac
      901 ttttcggatt tacaatttag tcctcaagaa ctaactggag acacactaaa aggcaataca
      961 attggatttg aagtttacgg aaaacataat attgcagcat caactaaaaa ctgggaaatc
     1021 cgtcttcaat tagatgaacg attagctaaa tatgtagaaa aaattcaggt cgatccaaag
     1081 aaaggaattg gatcttctag acggaccttt gtaagaatta atgattcgct gggtagacct
     1141 acaaatattt ggaaggtcaa ttatattcgt gcaagtgatg gattatttgc tggggcagaa
     1201 acaaccgata cgcaaaccgc tcctaatggg gtaattacgt ttgaaaagag cctagatgaa
     1261 atttttaaag agataggcat agacaatctc aagaccgatc gtttaatgta tcgaatctac
     1321 ttggtaagtc atcaagacga tgataaaatt gtaccgggta ttgatagtac gggctacttt
     1381 ttaacagatt cagatgattt ctataatagc ttagatgtat ctgaaaataa tcctgaccag
     1441 tttaaacatg gatcagtaag tgcaaaatat gagaaaccta atactcaaac aaaagatggt
     1501 tcaggatcca caggtgctaa tggagctata atcttagatc ataagttaac gaaaaattat
     1561 aatttctcat attcatcttc cgcaaagggc acgccttggt atgctaatta caaaattgat
     1621 gaacgattag ttccatatgt agcgggtata cagatgcaca tggttcaggc tgacaaagta
     1681 acatatgatg tttcgtttga atccggaaca aaagtagctg atttagcaat cgagcgacgg
     1741 gaaggtcatg aaaattatgg cataggttca ataactaata atgatttaac taaacttatc
     1801 gattttgcta atgcaagtcc acgtccagtc gttattcgat atgttttaca attgactaag
     1861 ccattagatg aaatcttaga agatatgaaa gcaacagcac aagttgaaga aaataaacca
     1921 tttggtgaag atttcatctt tgattcttgg ttgtcggata cgaataaaaa attaatccag
     1981 aacacttatg gaacaggtta ttattatttg caagatattg atggtgatgg aaaccctgac
     2041 gataaagaag agagcggaga cacgaatcca tatatcggga aacctgaatt agaagaagta
     2101 tatgatgttg acacaacagt taaggggaaa gtattcatcc acgagttagc gggaacaggt
     2161 cacaaagccc aacttgttga taaagaaggt actgtattag cagaaaaaac tatcgctcca
     2221 aatgaaaaag atggggctcc aatttcagat actgtagaat ttgaatttac gggtgtagat
     2281 tcaagtaaat taatcgcgaa agatgaatta aaaatccaaa tcgtttctcc aggttttgat
     2341 aaaccagaag aaggttcaac cgttattaag gaatcaccaa aagcggttga taaacaaacc
     2401 gtggtagttg gatttaaacc agatgctaaa gaatcaattc ggaataataa aaacttacct
     2461 gaagatgcag agtattcatg gaaaacagag cctgatactt ctaacgttac tgatagtacg
     2521 aaaggtattg taactgttaa gatcggaaat cgaactttcg acgtggatgt agagtttgct
     2581 gtaaaagctt ctcaagctat ggaaaatgat gcaacatacg tacctataac aacaactcca
     2641 gaaacgacag ttcaaagtgg aaaacctaca tttgataaac cagatgttcc tttagctaaa
     2701 gatgcctttt caatttcaga tgtctataat aagtacttcg gtaatgcaag tgttgatgca
     2761 aacactggta ttgttacatt cactccagct aaaggtgtag gagaatcgga gccgattact
     2821 ggaacaattc ctattaaaat tgtttaccaa gatggttctg taggcacgac cgatttagca
     2881 gtaactgtaa gtaaagatat ttatgaaaat ccaggagaaa acattcctgc aggctaccac
     2941 aaagtaacct tcaccgcagg ggaaggaaca agtattgaaa gtggaacaac agtctttgca
     3001 gtgaaagacg gcgtaagctt gccagaagat aaacttccag tgttgaaagc aaaagatggt
     3061 tatacagatg cgaaatggcc agaagaagca actcaaccaa ttacagcaga tgatacagaa
     3121 tttgtatcaa gtgcaacaaa actggatgat atcattgaaa atccaggaga aaacattcct
     3181 gcaggctacc acaaagtaac cttcaccgca ggagaaggaa caagtattga aagtggaaca
     3241 acagtctttg cagtgaaaga cggcgtaagc ttaccagaag ataaacttcc agtgttgaaa
     3301 gcaaaagatg gttatacaga tgcgaaatgg ccagaggaag caactcaacc aattacagca
     3361 gatgatacag aatttgtatc aagtgcaaca aaactggatg ataaatctga cgctgacaaa
     3421 tataatcctg aaggtcaaaa agtgactaca gaattgaata aggaaccaga cgcatttgag
     3481 ggaattaaga acaaagaaga tttaccaaaa gatactaagt atacttggaa agaaaaagta
     3541 gatgttagcg cagctggaaa taaaaaaggg actgttgtag tgacatatcc agatggatca
     3601 tctgatgaag ttgaagtaga tgttacagtg acagacaatc gttctgacgc cgataaatat
     3661 gagccaacag tagaagg
//
LOCUS       DARBDV010000161         3221 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000161 DARBDV010000000
VERSION     DARBDV010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.168"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            301..585
                     /locus_tag="SCM56_002826"
     CDS             301..585
                     /locus_tag="SCM56_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111550.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="SCM56_002827"
     CDS             588..1004
                     /locus_tag="SCM56_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111551.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="SCM56_002828"
     CDS             complement(1141..1860)
                     /locus_tag="SCM56_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG1111552.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2535..2630
                     /locus_tag="SCM56_002829"
     CDS             2535..2630
                     /locus_tag="SCM56_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEG1111553.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2932..3177)
                     /locus_tag="SCM56_002830"
     CDS             complement(2932..3177)
                     /locus_tag="SCM56_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111554.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1074 a          599 c          503 g         1045 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtactc
     2041 agataaaaag ttgttagtac ccttctgtat ccgttgggag agtaaggctc aactgccccc
     2101 taaaaagggt tctaaaaaat ctttaaaaaa taaataaaat aaacgcgcgc gcgcgagcaa
     2161 aatttcccgc caaaaaatca cccacttttt aaaataaaca ggttcgcgca tgatattaaa
     2221 ctgccaatct agctccctat ggtcgctgta agaaggagtg atcgctacgc tctcacattt
     2281 tacttgcagc agtcgccttt aggctccttc gccaaaaata attcattagg gactcctttg
     2341 tggcgtcctg agagctcgtc taagccgttc taagacaaga ttcgataaaa actatgtcct
     2401 tcgttctaaa gcgccttaca ggcggtcagg gcaagcccta acaaccccaa ggcaataaaa
     2461 aacttaggct tatttttgct tttttattgt ataattgatt gtcgcctatc cgacagtctt
     2521 gcgactggaa ggaggtgtgt ttcgtgttca ctaactttat ttgctccctt cttgttgggc
     2581 ttttaagcgc tttgttcagt tactggttga ataaccgaaa caagaagtaa aacttaatta
     2641 aggcgactca gcccacaaaa aaagccccag agaatccgac ctctctgggg ctttcgtgtg
     2701 tttcgtgttc acttatttgc aatcaaatta taacagaaat aaatatttat ttcaaaaata
     2761 tcctacaaaa gtctaaaatc cgtaattatt tcttatatgc aacatttaca aataaagtcg
     2821 ctctgagagc tcgtattttt cgatttaaga cgttttattt agtttttggt gtatttcatt
     2881 ggtccaagct aaaaaactct tagaagcgat tttagagcca tttaaaccca cttaattata
     2941 ttctccatga aattcgctcc atgcgtcaaa agctgcgtct gaaattccgc cgtcttcgtt
     3001 cactagttgc attaattttt ccaacgatcg ttttctttcg ggtgtcatat ttttatattc
     3061 caacaatttt tcgtctgtta aatcgaactt gattgcttga attgcttgct ctaatttttc
     3121 taagttttga aaatattcgt aaggaaattt ttccattttt tctttctcca gtctcacaat
     3181 ttttctagaa ataaaatcac ttctttgccg tctatactct c
//
LOCUS       DARBDV010000162         3170 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000162 DARBDV010000000
VERSION     DARBDV010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.169"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..485
                     /locus_tag="SCM56_002831"
     CDS             <1..485
                     /locus_tag="SCM56_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111555.1"
                     /translation="EEVFVGDKCIFFDQPLLSDGYGIDETDIFAKVNSIFSGKKETLI
                     KLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLISPISTISFSPSMMFEKTINS
                     IMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEEKILLPENWAELRKLVEYGII
                     "
     gene            472..1893
                     /locus_tag="SCM56_002832"
     CDS             472..1893
                     /locus_tag="SCM56_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEG1111556.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            1906..2913
                     /locus_tag="SCM56_002833"
     CDS             1906..2913
                     /locus_tag="SCM56_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEG1111557.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            2925..>3170
                     /locus_tag="SCM56_002834"
     CDS             2925..>3170
                     /locus_tag="SCM56_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HEG1111558.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDF"
BASE COUNT         1125 a          403 c          530 g         1112 t
ORIGIN      
        1 tagaagaagt ttttgtcgga gataaatgta tcttttttga tcaaccttta ttatctgatg
       61 gttatggcat agatgaaaca gatattttcg ctaaagtaaa tagtatattt tctggaaaaa
      121 aagaaacttt aattaagctt catcctagag caaggacaaa tcgttatggt gaaaaaaaca
      181 tattgaattc cagtttaccg tttgaagtct tgctacttga aaatgacttc tcttcaacac
      241 ttttgattag tcctatatct acaatttctt tttcaccatc tatgatgttt gaaaagacga
      301 tcaactctat tatgttggct aaattaattt taaatgaata tacagcatta tctgaagata
      361 aaaaagaaac attgacaaat attgttcttt tctgtgaaca ttataaccgt ctatcagaag
      421 aaaaaatttt actcccagaa aactgggcag aattaagaaa gttggtagag tatgggatta
      481 tttaacacac tttccaagtc aaaattagtt aagtccagtt tctggtatac tatagggaat
      541 ttttttatta agggaatttc attcataact attcctattt ttgtaaatat tactactcaa
      601 gcagaatatg gtttgattaa taatttcact tcatttgtat caattttttc gattcttgtt
      661 gggctaagcc taaattcggc aattaataat gcaagttttg actataaaga tgacataaat
      721 gaatttatgt catcggtact ttctctagct acactttcgt taattgtgtt cttattaata
      781 gggaacatat attttttgtt ttcaaatgct ttttttgata tgaaccaact tgtatttaat
      841 ttgatgttat tacaaagttt tgctagtttt gtaattagtt atatccagtc ttattttacg
      901 attaacttta accattttaa atatttgatt ttatctttta tatctacttt tttgaatatt
      961 gcaatctctg tagctttgat gcagacatta tattttgatg ataaatatgt tggcagagta
     1021 gtcggcggaa cactgtcatt tgtgttgata ggtttagtaa tttatttaag aataatgata
     1081 aagggtgctc atttttttga taaaaaacag tggagatacg ctttaaaaat ctctttgccg
     1141 ctaatcccac attccttagc aaatatagtt ttagcacaat tcgatagaat tatgattaat
     1201 tcttatttag gtcctgctgc ggggggaata tattcctaca ttagtaacat aggtattatt
     1261 ctaagcgtaa tatggatgtc tactaataac gcctgggttc catggttcta tggtgagatg
     1321 agtaaaaata atgaaaaaaa agtgaagaaa gttagtaact attatttagt agtctttact
     1381 ttgattacga tgattgtaat ggtagtttcg attgatttag caaaatttat ggctccgaaa
     1441 gactattatt ctgggttatc tttagtggtt ccgataacct taggctattt ctttcagttt
     1501 ttatatagtt taccagttaa tgctgaattt tatgagaaaa aaacatcgtt tattgcgatt
     1561 ggtacagtag caagtgctat agttaatata ctattaaaca tgattttcat acctaggtta
     1621 ggaataattg ctgctggata cacaaccgta tttacgtatt ttttactttt tatttttcat
     1681 tataatatcg ctaaaaaaat tacgaataaa caattatttg acacaaaaaa aatatgtatc
     1741 ataataattt ttgtatcaat tttttctttg atacttcata ttttaatcaa tcaaatgttg
     1801 attcgttacg ttttgacatc attattgata attagtttag ttatatattt tttaaagatt
     1861 aagaatcgaa ttatagatga ctataattca taggaggaga tataagtgtt taaaggaaaa
     1921 acaatattag taactggcgg aactggttcc attggaagtg aaattgttcg tcagctttta
     1981 aaatatgaac caaaagctat cagaatttat agtagaggtg aagaaaaaca attttatatg
     2041 caacaagagc tctcaaatca cactaatttg agatttttaa ttggtgatat cagagactct
     2101 caaaggattg attatgcatg tcgtgatgtg gatattattt ttcatgctgc cgcaatgaaa
     2161 cacgttccag caagtgaata taatcctatg gaagctgtaa aaacaaatgt gatgggaact
     2221 caaaatgtaa tagatgcagc gatcaaaaat aatgtaaaac attttgtagg tatcagtaca
     2281 gataaagtag tgagtcctac taacaccatg ggtgcgacaa agttattgag tgaaaaatta
     2341 ataatagctg ccaatcagta taaaggaaaa acagaaacaa tattttcttg tgtaaggttt
     2401 ggaaatgtca tggggtcaag tggatctgtt attcctcttt ttagtaaaca aataaaaaac
     2461 aataaagata ttactttgac agatgttgat atgacacgat ttatgatgtc aataccggat
     2521 gctgcaagat tagttctgtc tgctgctatg aattcaaaat ttggagaaac atatgtctta
     2581 aaaatgccgg caatcaatat tttaacttta gctcaatcat taataaaaat atataatgat
     2641 acacatgatg aaaaatatag aggtgaaata aaagttgtag gtatcaggcc aggagaaaaa
     2701 ttatatgaag agctaatgac tacagacgaa attgaacgct catatgaaaa tgataaaatg
     2761 tatatcattc caccacacta tcatgctact gacgaactgt acaaagatta tgaaaaaatt
     2821 aaaatcactg catactcttc agatgcaaca gaattaatga gtggacagga atttattaga
     2881 atcgcaaaag agaataatgt cgtgacttgg taaggagttt tatgatgaaa tcagaagtat
     2941 atgtaattgc agaaattggt gtaaaccata atggaagtct agatttagct ttagaaagta
     3001 ttgataaagc taaagaatgt ggagctaacg cggttaaatt tcaaacattt aaaacgaata
     3061 agctagtttc caaaaaggct ccaatggcaa aatatcagaa ggataatctt aaagaggaca
     3121 gttctcaatt tgaaatgcta aaaaaattag aattatctca atctgatttt
//
LOCUS       DARBDV010000163         3128 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000163 DARBDV010000000
VERSION     DARBDV010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.170"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            103..1347
                     /locus_tag="SCM56_002835"
                     /pseudo
     CDS             103..1347
                     /locus_tag="SCM56_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            1360..1992
                     /locus_tag="SCM56_002836"
     CDS             1360..1992
                     /locus_tag="SCM56_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEG1111559.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2197..2424
                     /locus_tag="SCM56_002837"
     CDS             2197..2424
                     /locus_tag="SCM56_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEG1111560.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2493..2759
                     /locus_tag="SCM56_002838"
     CDS             2493..2759
                     /locus_tag="SCM56_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEG1111561.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2749..3018
                     /locus_tag="SCM56_002839"
     CDS             2749..3018
                     /locus_tag="SCM56_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEG1111562.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1101 a          466 c          617 g          944 t
ORIGIN      
        1 tgaaattgct ttcctttaaa atgactcatc gtcattcctc ctgctatctt tttctattat
       61 tctaccttat ctgatagtag atttaaaact ttgcaacaga accagaaaac caaagatcag
      121 cttaacgaat tttttgctaa tgttcttgaa accccttatt ggtatgcaaa aaaaatctta
      181 agccttttat ctgagtgttt agatcaacct gtgtatgaag aggacatttt ctttattacg
      241 ctatattttt atagtttgaa tgtcacgaat gttaaaacag gtattaagag tattgtgcta
      301 gctcatggtt attctacagc tagtagtatg gcgaatgttg ccaaccgtat gctgaataag
      361 aacgtttttc aagcttatga tatgccttta gatattacat tagagaaaat tgaagcaaaa
      421 ttgattcaat tccttggaga ttatcgaaca gattcaggct tgattttatt ggtagatatg
      481 ggttcactaa accaactagg cggtttatta gtgaatcatc taaatggacc actgttaatc
      541 attgatcata tcaatacacc gttattattg gaagtcggcc aacaagtatt aaacaataaa
      601 agtatcaccg aaataggagc agaaattgaa acaagtctta ctttcaaaaa gcagttacta
      661 cttcccaaga aaaagaaaaa gaaagccatc attacttgtt gttatacagg gattggtagt
      721 gcgacccaga ttcaggagat tttaacgaaa tgcttgaaag atgcagttag agaattagta
      781 attattccgt atgattacaa aaagctgtta gaaaacaaga attttgagac tccttttcaa
      841 ttatacgatg tcatcatgat tgtaggtaca gaagatccca aaattaatca gattccatat
      901 attggattag atcgcctgat taatggggga agcattggcg aatttgttca ttacttggaa
      961 aaagagctga ctattgaagt gaacgattta caaaaagaat taattttcaa tttttcaatt
     1021 agtaaaattg tcgaaaattt aaccattttg gatccagata aggtgctatc ttttgtacaa
     1081 aaagcaatta aagaattatc gaagttatta aattttgatt tttctaataa tcagctgttt
     1141 ttgttgtaca ttcactgtag ttgtatgatt gaacgaattg tacgtaaaga aagtgtggat
     1201 gagcaagagg acattgaaga atatatgcaa gcaaatggag aaataatgga taaaattcat
     1261 agcgcttttt ttgagattga aagggaatac acaattgaaa taccagattt agagttgcga
     1321 ttattgagcg atattgttca aggataggag tgaattcgaa tgacaatggg agtaattttt
     1381 gatatggatg gggtactcat agatagcgag acattttatt ttgagagaag aatgaacttc
     1441 tttcaagaac tggagataga tccaggatca aaccagattg atgattatgt ggggaaaaca
     1501 gaagctgcaa tatgggaaac attagttcct gaggacaaat cattaagaga ggaactttat
     1561 ccgaaatatg tagattatcg aaaaacgcat ccaattgatt tcgctaaagc tttacgaaaa
     1621 gaagtgaaac cgttacttga cttcctcaag aagcgggata taccattggc actggcatct
     1681 tcttctccag ctgtcgagat agagagtatg ctcattcaat gtgaacttac tgaatatttt
     1741 tcctttgtta taagtggtga gtcattaaaa caaagcaaac cacatcccga aatatattta
     1801 aaagcgaaac agcaattaga ctgtgacttc tttattgcag tagaggattc aacgattggt
     1861 attaaatcag caaaggcagc aaatatctat acggcggcac taaaacaacc aatgcaaatc
     1921 gatcagagtg aagcagacat acaaattgaa agtctacaag aactggaaca aattgtttta
     1981 gaaaaaggtt agtgaattaa tggaaataag gctatatatc tgtgctagct attttgacta
     2041 accaaaaaaa cttgctataa ttcaggtgta gtaaaaggtg ctggtaacac cttttacgaa
     2101 aatgcttcgg cagtgataat gggattggct ttttaagttt tttattttgt tcgcaatccc
     2161 taaggtcatg tgaatgatcc tagggattgc tagaatattt tgcaacagaa ccaaaatacg
     2221 aatagttatc aagcagccat attgactgcg gtcaatttag gaggggatac tgatactact
     2281 gctgccgtaa ctggtgctct tgcgggtatt ctttacgggg gtgaagtgat ccctgaaaat
     2341 tggagaaaat cgcttcaaaa tgaccagctt cttaaaacga tcagtcagag attttgcgat
     2401 accgtgctat ctcaaagaat ttgaataatg gatatgatgc aaatcttgtt tgtactaacg
     2461 ttgtattgac aaaaaggatt ttatttagta caatgttagt acaaatgatg aacggaggta
     2521 gaatcatgtc tcaatcaacc attacattta ggattcccga cgatgaaaaa gaactggtat
     2581 tagaatatgc aaaagcacac aattcttcat tgaccgaact ttatcgaaat gctgtactgg
     2641 ataaaatcga agatgaaatc gatctacgga ctttacggag tgcaattgaa gtttccaaag
     2701 agaaaaaaga gagtggtatt tctcaagagg aaatggagaa actgctaaat gaattataaa
     2761 gtcatctaca tgccgcaagc ccagaagcaa ttaaaaaaaa tggacagaaa ccaagctaaa
     2821 ataattgtcg cctggatcag aaagaattta gaaaacacga ctgacccgcg tcagcacgga
     2881 aaaggtttga ctggaaataa gagtggggaa tggcgctatc gaattggaaa ctatcgaata
     2941 ttggctaata tttgtgattc tgaaattgaa atccagatat tctcaattgg acatagaagt
     3001 acaatatata aaagataacc gtttttggaa cgaagtagag tttcgaaagt caatgtataa
     3061 tcttttgaat tttgcataat accacaggta gaaactaccc gatactatcg gttggtttct
     3121 gctttttt
//
LOCUS       DARBDV010000164         3055 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000164 DARBDV010000000
VERSION     DARBDV010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.171"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(1..355)
                     /locus_tag="SCM56_002840"
                     /pseudo
     CDS             complement(1..355)
                     /locus_tag="SCM56_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(615..1163)
                     /locus_tag="SCM56_002841"
     CDS             complement(615..1163)
                     /locus_tag="SCM56_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111563.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1164..2021)
                     /locus_tag="SCM56_002842"
     CDS             complement(1164..2021)
                     /locus_tag="SCM56_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEG1111564.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2307..2593)
                     /locus_tag="SCM56_002843"
                     /pseudo
     CDS             complement(2307..2593)
                     /locus_tag="SCM56_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2583..2912)
                     /locus_tag="SCM56_002844"
     CDS             complement(2583..2912)
                     /locus_tag="SCM56_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEG1111565.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          893 a          620 c          384 g         1158 t
ORIGIN      
        1 ttaaatgttc ttactgcctc ttccacacta atttcttcgt tcaatttcac taataatact
       61 tgatctcgct tattataatc gctttcattt atcctaagag tcaaatcttt ttccaccata
      121 acttttacac ccaataaatt ttgcggttta tttttctctg ttgaaattct ttgtgtagcc
      181 tttaaactga caattagctc attgggtaca tacttatttg agagtcatct aattggctca
      241 taatcctttc tttttggtta gtatctgcag ttacttgttt agtatgagcc atgaacccaa
      301 acatactcac cccaacaacc aatcctaaat atattttctt tttaaacttt ttcatctaca
      361 tctcctccta atagttttat tttagagtta gctctattac ttatttacta actcaacaac
      421 agattatcat acacttgtta atgaaatcac aatatctcat taaaacacac cctaaaatca
      481 taaccgttcc tatgctcatt ttttggattt tttaatcacg taaccagaaa aataaatccc
      541 ctctatttca cgcaaatcaa agaagaacta agtaactacc gtttaaaggt agttacttag
      601 ttcttctttg attattatct aactttatta ttcattcccc accctttgcg cagtgagtct
      661 tcggtatctt gttttatctg actggtagtt cttattattt ctccacgatc atctaattct
      721 ggaacaattg gatatttttc tctgaacaag tctagttctt tcaccacgtc attaaacaaa
      781 tcttgcttat cttcttgcgt aagattctgt ttaatatact catctaaagc ttttctaagc
      841 aattcatctc ttgtcatatt agtctcatac ttttctacta aaactctgga agtcgcattt
      901 aacaaagtcg acagttttaa aggatgcttg gtggaataaa ccttatcaaa ctttctgatt
      961 ggtgcgccaa ctttttttgt tcttcctcta ctctgtcttt caacattgga ggaagttttt
     1021 atttcttgaa cttggtgcac ttctaattgc ttattttcag atggtgtaat tgcttgctgc
     1081 tttttctttg cttctgcttc tttttttatt ttggcgagtc ctttgttgtt cccaaaaata
     1141 tcaagtcctt tgatgtcagc caattactcc acctccgcat accaattctc gtgttcttcc
     1201 aattctttca aaatactgat aaataattcg tggcttttgc gatcccattg ttttaagaga
     1261 cctttctttg tcttctccat cgtaatacct gatccgtcgt atcgttttaa gcgcgcattt
     1321 ttgagtacaa catttttaat caaatgttcc ccataaatct cttgggcttg tttatacatt
     1381 tcctgatcta ctgcgtcacc gctttccaac atgaaaggaa taacaccagc aacatataaa
     1441 tttgtatcaa attctttcac aaaaaagtcc atatactcaa gatattgcat cgcatttctc
     1501 atcgatttta cttgggtctg taagacgacg attgcccaat ccgcaatgta catcgcggat
     1561 ttactatata cagaaataga aggtgggaca tctaaataga tgacgtcaaa ctcatcaaat
     1621 aatggttcta gcttttcttt catatatttg aattgggata atgaatcatt atattggtct
     1681 ttgatccaat catcatacat ttcaaaggac atgtctgctg gaagtatata gaggttattg
     1741 attactttat agacagcctc tttaggtgga agattgttta tcaacacgtc agtcacagta
     1801 ttttcgtaaa caatcggttc accttctccg cctccttctt cgttagtgaa gttcatatgc
     1861 gttaagttca tcacatcaga agtatttcct tgcatatcca agtcaattat caaggttctt
     1921 tttccttcat taactgctga ttcaaaccct aacatttgcg cgcacgtact tttacctacg
     1981 ccacctttaa aattaccgat tacatatttc ttcgctacca cgttcctacc tcttttcttt
     2041 tagcaatatt catttgatac tattataaca tatttatttt tagttactac caaaaaaaag
     2101 atatatatgt actaccttat aattaataag taaaaggtag tacataaata aggaaaagct
     2161 gaaatatatt gatttatagc tttcctcaaa gtaatgacgt tatttaattt atcgagaagc
     2221 agagaatact cgcgacttta gtcatgtaga cgaatcagat tttcgataaa aaagaagccg
     2281 tccttcttaa gaacgacaac aaatcactag attaattttt tcaactgttc ataaaagttt
     2341 tcatgcgttc cacataacaa tactggaata accttgtttt ctataatctc gtaagctact
     2401 ctatatgttg tgcccgcata tttgaaccca ctagaccaaa tacctgataa atccccagtt
     2461 ttttgttcac ccttatatgg atcaatcgca atttcatcat aaattaaatt caaaaatttt
     2521 tctttcaatg gcttctcttt tagcttttta aatacttaat gacatgtttt gaaggaacga
     2581 tttcataagc caatttctgt tgccaactcc tctttagtca ttactttaga attagtaagt
     2641 gtatcttcag caatatctct aaaggaagaa tgcattttgt tttttctatt cttaaattct
     2701 acaagaatct catctttatc gtagccttct gcaattatat cagacaaaat atcttcgcta
     2761 aaatcataaa attctttttg tggttcaaca aattcataaa gaataccatt ctctaccaac
     2821 tttgcttcaa cttcgactcc attaggaaca ttaaaatctt taggtactgt caacatgatt
     2881 gaattgcctt gttttcttgt ttttgtaatc atttaaatcc ctcatttcta acagtatgta
     2941 attacttagt aaatacatta taccacgaga taatgtattt acaagtaatt actgtgactc
     3001 gtaatgcgtc cttgctgaat aaatttcaac cacgtgttca tcttcatcta cctta
//
LOCUS       DARBDV010000165         3010 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000165 DARBDV010000000
VERSION     DARBDV010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.172"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1412
                     /locus_tag="SCM56_002845"
     CDS             <1..1412
                     /locus_tag="SCM56_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG1111566.1"
                     /translation="PTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTN
                     TPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNV
                     TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNA
                     DKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTY
                     PDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYT
                     WKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTG
                     KTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNV
                     VVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGL
                     ILLGTLGLLGYKNRKKENE"
     gene            1546..2889
                     /locus_tag="SCM56_002846"
     CDS             1546..2889
                     /locus_tag="SCM56_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEG1111567.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
BASE COUNT         1197 a          406 c          578 g          829 t
ORIGIN      
        1 aaccaacagt agaaggcgaa aaagtagaag ttgggggcac agtagatcta acagataatg
       61 ttactaactt gccagcgtta ccagaaggaa caacagtaac agatgttact cccggcggta
      121 caattgatac caacacacca ggtaattacg aaggtgtcat tgaagtaacg tatccagacg
      181 gtacgaaaga tacggtgaaa gttccagtag aagtgacaga caatcgttct gacgccgata
      241 aatatgaacc aacagtagaa ggcgaaaaag tagaaattgg aggcacagta gatttaacag
      301 ataatgttac taacttgcca gcgttaccag aaggaacaac agtaacagat gttactcccg
      361 gcggtacaat tgataccaac acaccaggca attatgaagg tgtcattgaa gtaacgtatc
      421 cagacggtac gaaagatacg gtgaaagttc cagtagaagt gacagacaat cgttctaatg
      481 cagataaata caatcctgaa ggacaaaaag tgactacaga cttaaataaa gagccagatg
      541 catctgaggg aattaagaac aaagaagatt taccaaaagg aactacttat gcttggaaag
      601 agaaagtaga tgttagcaca gctggaaata aaaaaggtac tattgtagtg acatatccag
      661 atggatcatc tgatgaagtt gaagtagacg ttacagtgac agatactcgt tccgatgcag
      721 ataaatataa tcctgaagga caaaaagtga ctacagactt aaataaagag ccagatgcat
      781 ctgagggaat taagaacaaa gaagatctac caaaaggaac tacttatact tggaaagaaa
      841 aagtagatgt tagtacagct ggaaacaaaa aaggtactgt agtagtaaca tatccagatg
      901 gatctaaaga agaagtagaa gttactattt ctgtagtaga taaaaaagca ccgaataaac
      961 ctcaagttga tcctattaca gagggtgacc aaattgttac tggtaaaact gaaccaaatg
     1021 cagaggtgac agttacatta ccagacggaa gtcaacacca tgggacagct gataaaaatg
     1081 gtaattttac agttaaagtt cctaaattag aggcagggac aaaagtgata gtaactgcaa
     1141 ctgatgaatc tggtaatact agtgaaccta ctaatgtagt tgtttcttcc aacaaaaaag
     1201 acagtggaaa aaacggtagt aaaggtagta agactgacaa tcaaggtagc aacagcaatc
     1261 aagacaaaaa tagaggcaaa agccagtcct caaaagtttt tcctaagacg ggagagagtg
     1321 acagtaatat ctttactatt agcggtggtt taatactgtt aggaacgtta ggattattag
     1381 ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa tttcttaagt cttctctgag
     1441 aagaggaaga atctttttct tcctcttttt gcttttttat gatgggaaat aagtgttagt
     1501 taaagatgaa tatacttttt atagaaagga aaagaatgta attaagtgga aaaaatagta
     1561 attattggtt caaaccattc agggctagcc gcagctcgat ttctaaactt aagcgattca
     1621 aattatgaag ttattctaat cgataaaaaa tcagaaatta gttacttgga atgtgggaca
     1681 tcgttattta taaaaggaga agtaagtaat ttagaagatc ttttttatca aaatagaatg
     1741 acactaaatt ctgagataaa aaagttattt ctagagacag aagttgacaa aattgatatt
     1801 caaaataaaa agattagttg tgtaaaaaaa gatggtacaa gattctctca agaatatgac
     1861 aaagttatat tagctacagg ttcaaagcaa attatgctag atataccggg gaataattta
     1921 gctaatattt tttcgataaa gaattttcca aatgcaaaag agatttttaa agctttggat
     1981 gatagtacta ttaaaaaagt aggaattatt ggagcaggat atattggagt tgaaattgct
     2041 gaagccataa gaaaaagagg gaaagaagta tatcttttcg atgttgcaga tagagtgttg
     2101 tcgacttatt acgaccgtag tttttcagat aaagttgaag aaatactatc aaaaaatgga
     2161 atacatcttt gtctatcaga aagtcctaaa aaatatttag gaagaaaaaa gattgaaaaa
     2221 atagtcacta caagtggtac ccaatactct atggatatgg tcgtacaagc tataggattt
     2281 ctatctaata gtccaattgg ggaaaaggaa ctcttgagag atgttagtgg agcatatcta
     2341 actaatgaat accagcaaac aaatataaaa gatatatatg ctattgggga ttgtgctaca
     2401 acttttttta cgtccattaa tagaagtagt gttgattttt ctatttctaa tgctctaaga
     2461 acagcttata ttgcaactca acatattaac aatcaagatt ttacaccttc tggttcccaa
     2521 tttacaaatg gcttttctat atttaattac aatttttacg cagcaggttt gaatttaaaa
     2581 acagcacgat tattaagtga gtctatagat tatatagaaa tagaagattt cattagacct
     2641 gcttttttga gaaaaaatag tcgtgtaaaa ttaagaatta gctttgaaaa aaatacaaag
     2701 agaatagtag gtgtacaact ttgtagtcaa gaagatttat ctggactatt atcaatgttt
     2761 tctcttgcta tagaagaaca aattacatta gacaaattga aaatgttaga ttatttattt
     2821 catcctagtt ttagccaacc ctataatttt attttaaagg cagtaacagg attaaacatt
     2881 gaaacataat cgttactaaa tgaaatcaag aaatatactg tattactcaa ttcattgaaa
     2941 atatttatca taatttttaa tagaaaacac tattaaagag aaaaaagaga ggtaaatcaa
     3001 atgaaaaaat
//
LOCUS       DARBDV010000166         2910 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000166 DARBDV010000000
VERSION     DARBDV010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.173"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            123..515
                     /locus_tag="SCM56_002847"
     CDS             123..515
                     /locus_tag="SCM56_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111568.1"
                     /translation="MLDDRLTCSNCGTLFYYEEVRNIVKHRKKEMPIRCPNCNEVIKR
                     KLSHGYFVSYELYEESKINLKKKPKYYKNLKTGAIITEKEFKNLVTEEESRFYNLSGN
                     ICPGFMDQIIENESIESIENYVPVDLPK"
     gene            complement(688..870)
                     /locus_tag="SCM56_002848"
                     /pseudo
     CDS             complement(688..870)
                     /locus_tag="SCM56_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            887..1054
                     /locus_tag="SCM56_002849"
     CDS             887..1054
                     /locus_tag="SCM56_002849"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111569.1"
                     /translation="MENTFELYNKTTVDKLIPILVPTDLNGHDVLLRFNGFLGEDYRY
                     LKISDPQPLTE"
     gene            complement(1097..1672)
                     /locus_tag="SCM56_002850"
     CDS             complement(1097..1672)
                     /locus_tag="SCM56_002850"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEG1111570.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1827..2353)
                     /locus_tag="SCM56_002851"
                     /pseudo
     CDS             complement(1827..2353)
                     /locus_tag="SCM56_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          938 a          487 c          461 g         1024 t
ORIGIN      
        1 attttttctt ttaagtaaaa aatataggtg attttttttg acttcatatt tataataaag
       61 acacgtaaat caattatttt tcattttact ttaactaata atgtttattt ggagggatta
      121 ttatgttaga tgatagattg acgtgttcta attgtggtac attattttac tatgaggaag
      181 ttagaaatat tgtaaaacat agaaaaaaag aaatgcctat acgttgtcca aattgcaatg
      241 aagttattaa gcgaaagttg tctcatggtt attttgtcag ctacgaacta tatgaagaaa
      301 gtaaaataaa tctgaaaaaa aaaccaaaat actataaaaa tttgaaaact ggagctatta
      361 taactgaaaa agaatttaaa aatttagtga ctgaagaaga aagcaggttt tataatttgt
      421 ctggtaatat ttgccctgga tttatggatc agattattga aaatgagtca attgaatcaa
      481 ttgaaaacta tgttcctgtt gacttaccaa aatagaaaaa ataatcctgt ttttacagtg
      541 aattatttta attttgattt tcaataaaaa tatattacaa taataatcgt aatagctaat
      601 gaaacaattt ctcaatcttt ttaccactcc aaacagggag cgtcaataat tttgtgtaaa
      661 taactcgtcc ttctgcaaaa taatgggtta ctcagtaaac attgaagcta atgtatccgt
      721 cacttgttgg aagcctttat ggcttctatt taagaatttt tgattgtatg tatcaaagat
      781 ggatactagg aaacgctcta gtgattcttc attttgaaac tgctcttttc tgcggctgta
      841 tttcttgatt tgtttattga aagattcgat tgtagaacca gaaggaatgg agaacacttt
      901 tgaactttac aacaaaacta ctgttgataa attaattcca attttagttc caactgattt
      961 aaatggtcat gatgttttac ttcgttttaa cggattttta ggtgaggatt atagatacct
     1021 aaaaatttct gatcctcaac ctttaacaga ataataaaaa acgtgtaatc aattatttga
     1081 ttacacgttt tttatttcaa tcttcgttgt agtagtggtt gcgacatacc gaaaggtaat
     1141 cttcattacc tcctactaca atctgttcac ctgaataaac tggaatacca tcaacaatac
     1201 gagcattcat tacagccttt ttattgcaaa aatggcaaat agttttaatt tcctcaattt
     1261 tatcagcaaa tgtcaataat gcttcggatc ccttgaagag attattttga aaatcattct
     1321 tcaatccata gcaaattact ggtacttcct cattcattac gataagtgat agatcaataa
     1381 tattctctct acttaaaaat tgagactcat cgaccaaaat acaatcaact tttccgtact
     1441 gatcaataat ctccactaat tctccgtaca catccatttc agaagtaatt gcaatagctg
     1501 gtttcgaaac acctatacga ctctcaactt ttccaaatcc tgatcgagta tctattgcgc
     1561 ttgtcattat gataactctc ttcccttgtt cttcataatt atgagccact ttcagtagct
     1621 caatactctt tccactattc attgttccat atctgtaaaa taattgtgcc attaaattta
     1681 ttcccttctt ttacaattca attattcctt aattccatct gcttttatgc agctgctcgt
     1741 ttattccttg atatgtctat aacatctact atccctaaaa ccaatttata agccattaga
     1801 gatggtattc ctgaacatct ctcatattgt tttttgagtg gtatatcatc tgaaaaactg
     1861 aaggaatcat gaaaatagcg acatacttat catttagagt tctaacttta aatgcattta
     1921 cttcttcagt cagcacttta gtcatgttgg acatggtttg gggcgtgtag tgatgcccat
     1981 acattttctc aatcaagtct gcgatttccg acatggtaat accttttcgg aagaggtgaa
     2041 tgacggtctc ctctaatgtg tcattcgtcc gtctataagc aggaacagtc tgttgcttga
     2101 actcgccatt gcggtcgcgc ggaatctgga gatgaagttc cccatactcg gtcttgaccg
     2161 tacggtcata ggagccgtta cgagaattac ccgtgttaaa accaatgcga tcgtactttt
     2221 cgtaatcgag gaaagccgtt aactccgttt tgagaagcgt attgacagct ttttccaaat
     2281 ggacacggaa taatccattc aaatcgcctt tgttcgctag agtctttagg atttctgtag
     2341 taaaatcgtt catatggaag tcctcttttc tgtgaatgtg ttgtggtaac tctattctac
     2401 agaagggact tccttttttc tatttacaca aaatatttta cactctcaac aaaagtatct
     2461 tcgattaata cttgctcaag ttatgtgcag ctacgaaaaa gtaggtaccg aggtactttc
     2521 tttttcgcag ctttttttgg acttatgaga agtacattcg ctggaagaat acgatataaa
     2581 acgagctttt tcactaaaga tattaataga gtgaaagaaa caagcttttg ttgtcagttt
     2641 tttcactttt ctaaaaatga aaataaaaaa ttctggaagt ggagaagaat tttgcttgct
     2701 atactttgtc ttgtagaaaa gaggtagaca aaataatgca agctcaaata agcgattttc
     2761 cataaccttg gtaggaatga caaattaaat aggaaaggga agtaataata tgaataaaaa
     2821 aaagttatct ctattcggtt tttttgccct gacagcttcc atggttttga ctgtttatga
     2881 gtatccgacc tttgcaacat cacaattaca
//
LOCUS       DARBDV010000167         2899 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000167 DARBDV010000000
VERSION     DARBDV010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.174"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..129)
                     /locus_tag="SCM56_002852"
     CDS             complement(<1..129)
                     /locus_tag="SCM56_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain containing protein"
                     /protein_id="HEG1111571.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYN"
     gene            199..549
                     /locus_tag="SCM56_002853"
     CDS             199..549
                     /locus_tag="SCM56_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1111572.1"
                     /translation="MSRLIIQTAFYKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEEL
                     VMLFLDPDFIGKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLD
                     GFGKPYPITHLVKK"
     gene            963..1781
                     /locus_tag="SCM56_002854"
     CDS             963..1781
                     /locus_tag="SCM56_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEG1111573.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1854..2213)
                     /locus_tag="SCM56_002855"
     CDS             complement(1854..2213)
                     /locus_tag="SCM56_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEG1111574.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          982 a          409 c          518 g          990 t
ORIGIN      
        1 gttatagaca ttgtaaattg tttggcgtcc acgtttaagc tgcttcgcaa cgatggcaac
       61 cggagtttct tgatgaaaat atgcttctat cattacaagc tcgtttggtg taagatgggt
      121 ataagtcatt tatgttcact ctccttgtat gctttagcgg gtattacaat ttgagtgtaa
      181 cataaatggc ttttttattt gtctcgctta attatacaaa cggcgtttta taaagcaatc
      241 attcctgaga acttagatga ggttaaactt tatttatggg aagataacgg agaaattgtc
      301 ggttttagtg gtgtaagtgc agaagaatta gttatgctat ttcttgatcc agattttatt
      361 ggtaagcgat atggtagtca aatattaatt gagctgattg aaactgaaga tataaaaaga
      421 attgatgtta atagtcaaaa taaatatgca aaacattttt atttaagcca tggatttgaa
      481 attgaatctg aagaagactt agatggtttt ggtaaaccat accctataac gcatttagta
      541 aaaaaataat tcatgaatgg tttattacct gattgaacat gaataaaagt aatattttta
      601 ttttgatggc ctatttctag gttatgaatt atccagatca aatttgtgta ttacattcag
      661 taataaggtg cttatcttag gatcaactta tgatactagc tgaccaagga tgtaccagtt
      721 atagaaaatc agttaaagta aaaatgataa aaatcttatg tcaacactga tttaggctga
      781 cctgtgggtt aggagaaaga caggtcggta gcttttaata caaaaaatgg cttgtatgta
      841 tctagatagt taactgaaat tagaaatgtt cttattttta acggagtaag aaaaataggc
      901 tgctttaggt ctgaatactt tttcaatata atgaagatat caaaggcgac aggagtgttt
      961 ttatgaataa gaatcctcat aatctaaagt tggtagcagt agatatggat ggaacatttg
     1021 ctcgtactga ttacacatac gatatcccaa ggttcaaggc tgttttatcg cgaatgaaag
     1081 ctgttgattg tcaatttgtt gttgcaagtg gaaatcaata ctaccaattg agatcgttat
     1141 ttccaaatta ttatgatgaa ttaagttttg ttgctgaaaa tggtgctttt gttaaagata
     1201 aaacagaggt actattttca gccgatatgc ctagaaaagc gatcgaggtg gtccttgatt
     1261 tatgtaaaga ctatcctgaa gtaaaaaatg tgatgtgtgg tctgaatagt gcctactgtg
     1321 aaagaggaaa ggtgagtcaa gcattttttg atctaaccaa tatctattac catagattag
     1381 aatgggtagc tgattttgca gaagttcaag ataaaatttt aaaatttgct ccgactatgc
     1441 ctgttgaaaa aaccgaattt tacgttgaat tgttccgtga aaaattagcc gggctattag
     1501 tacctaccag tagtggtcat ggttctgtag acttgattct ttctgagtgc cataaaactt
     1561 tcggcttgaa aatgttagtt gagcgcttgg ggattaatcc tgaccagtgc gttgcttttg
     1621 gagatggggg caatgacatt gagatgttgg agtattgtgg tctgagttat gaaatggata
     1681 atgcaacgga agctgttaag caagttgcta agcatcaatg tccttcaaat gacgaagacg
     1741 gtgttttagt gacgctcgat cgattgttcc ctaacacgta aacactaggt aattcatcaa
     1801 aaaacctcga tataagaaat attcgaggtt ttaaaactat atttagaaca gatttacgaa
     1861 gcgtctgatg agtcaaaata gacttggaca ttgctatcta aaataccact ttcattcttt
     1921 aaacgaattg tttgatttga tggagttacc tctaatacta aatgcccatt aataaaggta
     1981 gtatcgtatt cagatgtttt aactgttgct tcttcgcaag gtattgttaa atcatatgct
     2041 ggaatataat gttgtgtgga aactaataaa aaatgatcac caatcataca gcatccctct
     2101 gctgaagaaa attgattaga gccatcattt aaagttaaca agaatccatc ctttaaacga
     2161 ggtacttgct taaatacttc tggttgtact tctaaaaata aattatcgct cattttcacg
     2221 accaccatcg acttccacag ttgaaattga cattttattt aacacaccta ttccccctta
     2281 actataactc aattctatat aaaaaatatt ctattctcaa ataattgaac ttttcaaatc
     2341 gccaatttac ttcggcttta tcagagctca ttcatatcaa gagcgattaa cgctgtaaaa
     2401 attaaaccga agatctgata aattttctct attttctaca atatttaaaa cgcttcctta
     2461 gtctattata tatctatttc tcttaggagt atgtttagat gtttgaataa tagttgccca
     2521 actttaaagg ttattcttat aatattttac ttcattttcc aatagaaaat tcaaaggatt
     2581 actggttttt ctaaaatagt ttagtttaaa tacataagta tcctccgaca ctgtattagt
     2641 tcataatcat ctaaatacaa aattttcaaa taaaaatatt tcaagctcta tcgttgacga
     2701 atgtaaacga tacttataaa gtaatagaaa ttgattacat ttgtaaactt aaaatggtta
     2761 atttatctca cataaaacta agaataaaat aatgattaaa agtggttagt ttttatacga
     2821 tttagcctat cggtaaaaat gaattagaga gtgtaaaata ttttgtgtaa atgaaaaaat
     2881 ccatacaaaa aaggaagtc
//
LOCUS       DARBDV010000168         2826 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000168 DARBDV010000000
VERSION     DARBDV010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.176"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            798..1052
                     /locus_tag="SCM56_002856"
                     /pseudo
     CDS             798..1052
                     /locus_tag="SCM56_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1122..1634
                     /locus_tag="SCM56_002857"
     CDS             1122..1634
                     /locus_tag="SCM56_002857"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1111575.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1631..2668
                     /locus_tag="SCM56_002858"
     CDS             1631..2668
                     /locus_tag="SCM56_002858"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG1111576.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1022 a          496 c          564 g          744 t
ORIGIN      
        1 ttgatgacca cgaaagtgca tggcgatatt tgatgttgat actttatgca gtaccctatg
       61 tattcttcgc tatcattctg tggctcatag cattgttttt tagagtattt aatgaccttt
      121 aggtagattt atatataaaa agggctaaat acgcaaaata cccccttaaa atgcaaaata
      181 aatgcaaata agggggtaac tccttatgaa aaaacaacta atagtattat aacacgtttt
      241 tatgccgaga aaacttattg gttggaatgg gctatgtgtt agctaacttg ttagcgagtt
      301 ggttggactt gaattgggat taatcccaag aaagtaccaa ctcaacaaca cataaagccc
      361 tgtaggttcc gaccaataag gaaattggaa taaagcaacg aaaggagttg agtaaaatga
      421 gtgagaaaac gaagaaaatc attcgagtat cattagcttt ttcacaagta gtagtgcaat
      481 cattgatagt ttattatact taccagcaat ataaattatc taaagagaat taagttttga
      541 ttatgtaata agttttggta ctaaaaaaga cttgatctaa ttagaccaag tcttttgtta
      601 gtgttatatt aataacaaaa taaaaataag aagtcgctca ctccccgacc aaagtttgtg
      661 agcaaaataa acagtcgatg tttgtttctt aattgtatat cttgatatta aacgatttaa
      721 gattattctt caagatatat attcgcaaat gtaattggaa aagcgacttc ttaaacaaaa
      781 ttaaggagtc gctttttatg gcaaaaataa cgaaaaatga gcaagaaaat ttggtggaaa
      841 attacacaaa aaaaccggcg tactctaata gccggttaga cgcacatttc gtgtgcactt
      901 ctaatccgaa attgagcttt gacgctatga cgatagtcgg aaaccttaat aaaaacagcg
      961 ctaaaaagct atccgatttt atgagtttag atccacaaat tagactttgg gatatacttc
     1021 aaacaaaatt taaagctaaa gcactacaag accccctatg ttaggctagt tgtcgtatag
     1081 gataataagt tataataaag actatacgga ggatgattac catgacaaat aaaaacaatg
     1141 aacgttactc taccgaactg aaagcgacag ccatcaagaa aatgatgcca cccgaaaata
     1201 gggggattcg ccaattaagt caaactttgg gcattccaga agcgactctt tattcttggc
     1261 gtaaaaaagc tagaattcaa gggaatacta cacccggaac gaaaaaaaat gtcgaaaaat
     1321 ggtcaagtga agataaattt ttaattgtga tggaatcatt ctcaatgaac caagtagaac
     1381 ttgcggaata ttgccgaaag aagggcttat ttaaagaaca aattgaggct tggaagaaaa
     1441 cgtgcttatc tgcgaatgaa caggaagaaa atcgaacgag agaacttgca actgaactaa
     1501 aagaagagaa aaaacaagcg cgtcaattag agaaagaatt acgtcataag gaaaaagcat
     1561 tagcggaagc cgctgctctc ttattactaa gaaaaaaagc acacgcgatt tggggggacc
     1621 aagaggaaga atgatttcat cttcagatcg caaacaagct gttgaactta ttcaagaagc
     1681 caatcgcaat ggggcccgtc tcacctatgc ctgtaatgag ctgaatatta gcgttcgaac
     1741 ctacgaacga tggacgcgtg aaggtacgat agcgcatgat caacgccctt tggcgaaacg
     1801 tcctgtaccc aaaaataaac tgacggatca agaacgcgaa aaaataatag aaaccgtttc
     1861 aaaaaaagaa tttatgaatt tgccacctag ccaaattgta ccaaaacttg cggattgctc
     1921 gatttatatt gcatcagagt ctagcttcta tcgtgtattg cgagaaaaaa atatgcaaca
     1981 tcaccgtggc cgaagtcaag tttcacaaaa acgcataccg ccaagtcacc ttgcgacgaa
     2041 gccaaatgaa gtttggactt gggatattac ttggctaaaa ggtccaataa aaggactctt
     2101 ttaccggtta tatctcatca ttgatttatt tagccgtaaa atggtaggct gggagatttg
     2161 ggaatcagaa gaagctcgtc atgccgaaaa acttattaaa cagacggtgc ttaaggaacg
     2221 tatccaagga cagccgttgg ttttacactc cgataatggc agccctatga aagccgcgac
     2281 ttttcaagtc ttactcgaaa aattaggaat tcaaagttct tattcaagac cacgagtgag
     2341 taatgacaat ccttattcgg aagcggcttt ccgaacgtta aaatatcgac cagaatatcc
     2401 caataacggc tttaaaacaa tagatgatgc tcgaaaatgg acgaaagagt ttgtcgattg
     2461 gtataacgat tctcatttgc atagtgggat tcaatttgtg tctcctgcag cgtgtcatga
     2521 aggacgacat ctcgctattt tagagaaacg aaagcaggtt tacgaggacg ctaaaaagcg
     2581 ccatcctgaa cgctggtcaa aaggtgttcg aaattggact ccacatcaat cggtggcgtt
     2641 gaatccttta aaagaaacag aaagttaaca ggagcataat ctttcaagat tatcaaataa
     2701 ccctcgtcac ccaatagggg aaaaagttat tcttccccta ttggataacg aggccaaccg
     2761 accataggga ggttctgctt tttgataggt ggaaaataaa aatacgacaa ctatattgac
     2821 aaacac
//
LOCUS       DARBDV010000169         2706 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000169 DARBDV010000000
VERSION     DARBDV010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.178"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(1090..1608)
                     /locus_tag="SCM56_002859"
     CDS             complement(1090..1608)
                     /locus_tag="SCM56_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEG1111577.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT          899 a          479 c          386 g          942 t
ORIGIN      
        1 ataattgaag taaattacaa atagctataa ttcaaatcca actatataga aaacgtaaat
       61 tttctatata gttggattaa gataacgact atttatttta tcatattttt actataccct
      121 atacttttac ttggtatatt tatgaataat cgcaaattta cccctatact actagtttgt
      181 aagttagatt aataaaaata ctaaagttat ttgttctttt attaaataca cctacaactt
      241 ataaattgat ggtatttaga taacacgaaa gaataaaaat aacagctgat agtaaagttt
      301 gttgacgatt gctactcaat cgaaagtgag ttagtgcgta tgaacttgct tctgaaaact
      361 tatgaaatga aagacctttt acatcttgca gaaatcggta ctgggattaa actaagcaat
      421 aaaaactaat gttcccctca ttctttgaac ttctactatt tgatttattt attctgataa
      481 atgacgattt caccttcagg atcttcaaat cccgtataga atttatttaa gatctcttca
      541 gtatcagtaa taggataggc gataggccac ataccctttg atggttagaa taaactgctc
      601 aaaatctgta tcgtatgttg gctttagcat attaacaaag gctatctcca aattataaac
      661 cataagcgtt gaatcgttct taacaatttc aattcttcct aacccaaagt cagaagatat
      721 aattaccgga cttgccttac ccttcatgtg ggttgttgag attccatatt taaagggaat
      781 tgtcggtact atcggcggtc tgaaaaacat cccaaaaata aaaaaatctt tcccctgcta
      841 gtctcctaag tccctttgta gcggatatgg caccataatc tcattttatc gaagtgagtc
      901 atggtttatc tgttggccaa ccaaaattga tacaatctaa cgatgcaccc tctacggcat
      961 tctaggggag tgcacttgca ttcacccaat aaaaaccggc agttagcaaa gaacttatgt
     1021 agtcgtatgt tcttactgtt aacatacaag aggtctcctt tccacaattg cttagcagta
     1081 aacgggaatt tacaaagcta atctcttcca ttttcttatc tttgttctaa aagtcccaaa
     1141 tggagcaact gtatttacat gaataaattt atatacttcc catacagccg ttttggttgc
     1201 ctcgtctgcc cattttcttt tgtgtgcagt gaatagatct tcatcactca ttgaatcaat
     1261 catcatgtta atatcatcaa tattacgatt gagaatttcc tctaattctt tcaatggtag
     1321 atgtgcatag gtatttgtaa accacttata taactctcct aactgattcc atttaaattg
     1381 atcagaaggc gttttaacag ataatccatt tctttcatca ttttcccatt ttagaattaa
     1441 attcgtccaa ccaacttgat atgctaaatt ttcagcaggt gttctatcca cttcctcaac
     1501 tcttttatcc ttcaattctt caggaatagt ttcaaattct aaaatatatt tagtataagt
     1561 cctatttatt tcttttttta attcttcttt atttttatat gttctcatta tattacctcc
     1621 ataaattcct aattgtctcc tttaattcat ctataatttc atccacgata ttttaatccg
     1681 ttctacaatg catatctgat atgcattgac tgtctaactc atatgtgaat gatttataaa
     1741 atatcccaac gtctcatcta aagaaatgtt gatcgtcagc aaggagctaa aaaatacgaa
     1801 tatatttctt ttgtagtaaa atcggactgg tttgcatatc attttcttcg taaatatcaa
     1861 aatcatgtta actactatca attactaata aaaactacca ctttaagcgt taagaactgt
     1921 taagtttagt taagtgagga tagttcacaa aatccattgt taaacttcac gaaagtggta
     1981 aaactcaaaa tgctttatat aggattacag catcgctctt tcttcgattg cgcgttgggt
     2041 aaaaaatatt atgaagtaaa aattgacgat gacacgttcc ttacggctca tcagatgaaa
     2101 cagctacaaa aacgaaatga tcaactcgaa gaaaatttaa ttctaaaaaa gcgattgcca
     2161 tattcacgcc tcgctcgacg aactattaaa ggtcaagatt tacctgaaga tcatcctttt
     2221 taggaaattg aagcttttta tgttgcacaa tgcatttata atactacaat gatgttttca
     2281 ccagatattc ttctagggtg cggtgtaatg aaacagatac acttgcaaaa gaaaattcac
     2341 cgcgaattta ctaaaattat gaataattat gttaatcttc cgaaattttt ttccaaaatt
     2401 aagggatcaa gctggtgtta tagattgttt aaggttggca aaaaaataac ttctttttaa
     2461 acaattatag aaaaccgata ctctgttaag ttggttgaca cggtgtcgat ttttatgact
     2521 ttcttattct gaaacttcac taacggacag tacctaaaaa gtctatagta tggacgttta
     2581 cgagtactat tttatcaaaa atgctgctca tttcaactta tatgttttag tgaactaccc
     2641 ccacttagct tggataggct acaattaact agacagaaaa attaaggtgt gtagactaga
     2701 agaaaa
//
LOCUS       DARBDV010000170         2531 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000170 DARBDV010000000
VERSION     DARBDV010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.179"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..379)
                     /locus_tag="SCM56_002860"
     CDS             complement(<1..379)
                     /locus_tag="SCM56_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEG1111578.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFLNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPS"
     gene            598..1968
                     /locus_tag="SCM56_002861"
     CDS             598..1968
                     /locus_tag="SCM56_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HEG1111579.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            1989..2273
                     /locus_tag="SCM56_002862"
     CDS             1989..2273
                     /locus_tag="SCM56_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111580.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          859 a          380 c          522 g          770 t
ORIGIN      
        1 gactaggaaa actatctata attccgacta gttcaccttt ggtgagcttc ttgatgaaaa
       61 agtatcgaat aatcttgata gtgtaagcta agtttgaact taagcgagtg aatcgactcc
      121 tactaggaaa gtcaccatta agaaataaga cggaacaaac cgctctatac gtggctcttt
      181 ggctagtata gccattaatt atgccccata taacacaagc aataatcact gtatcgcgtt
      241 gcttcagttg atcagtattt cttcggtttt taatcttatc tggtacacaa ttgtggtata
      301 tgttagaaac agttctcata atttccttga atgttataaa agtatctgta taatgttcag
      361 tatattttga tgaacgcata tgtagtcctc atttcttggg agttttgaga ctactatatg
      421 cgttttttgt tgatttacca acatgtttag acattttcct ctaggaaaaa attaactagc
      481 accacgcgta ttgtttatat taaattagat gaccgtgata atgcacagca aatttttgaa
      541 agtatcaatt ctaccggtga aagattgact gcctcggatt tgattcgaaa cttcatcatg
      601 atggataaat ctaatgaaga gcagactacg ctttatacta aatattggcg taggttggaa
      661 gaagtctttg ataattctaa aggaatggaa gactttttca gatactacct tgctgcaata
      721 actggggagt attcagcaaa gcatattctt tatcaagcat tcaaagacta ttggcataaa
      781 gagcgcgatg tatcaagtga tgccgaattg ctccaaaaat tagtgcgata tgctggttat
      841 tttgcgagac tttactataa caaacctgaa ggaaaatact cagaagtatt gagcgatttc
      901 caaagtatgg aaagtatgat gcctgcacct tttgttttgg gattatctga atggtactat
      961 catgaccaat ttatcactga agaccaatat attgatgcga ttaaggtagt gaatgattat
     1021 cagcttagga gatattttaa tggggatgat acaagcagag tgtcaaaagc atttccttca
     1081 tatttgaaaa gtgttaggaa gtacgctaaa gctaatggat atgaaaatat cgttgatatt
     1141 gtaatttatg ttcttgttac tagaaatcaa tcaaataata tggcacttcc atctgacaag
     1201 gcattgaaat ctaatctatt gaatgccaat gcttatgcga tgagattggc ccgttggtta
     1261 cttgaaaaaa tagagaatcg agaaaattca gcaacacttg atatgagtaa cttgtccatt
     1321 gaacacatca tgccacagac tagtacttca tattgggaag agaaggctgg tacatcgggg
     1381 gaagaataca caggattagt aaataccatt ggtaatttaa cactcgttac aaatcctgat
     1441 aattcggccg cgggaaataa agactttgag accaaaaaga aaatctttga agataccctt
     1501 catattcgta tgaataaaga tttgtacgaa ttgactgagt ggacatcatc ggatatttct
     1561 gccagaagtg aagctctaat caatgaactg attactatgt atccctattt gagatcgtcg
     1621 ggcgactatg aacacgatgg gaatagggaa attttcttag aggcgcaagg aataaaagca
     1681 actgggtatt tgaacgaaga cgagaccgtg attatacatt caggttcaga aatttattct
     1741 aagattaaag atattgcatc tgattcgcta gatgaaactc gccaagagct gttggataat
     1801 ggaatcattg aagaaacaat tgggggacta caatttgttc aagattacac tgcctcatct
     1861 gtttcgaatg cagcagctct gctattaggg ggtagccgga atggttggga ctactggaaa
     1921 tatgataacg gaataagtat taatgattcg ctaagaaata agaaatagag catagaggtg
     1981 gtgttaggtt ggataagaac aaactaagat atgcaattct taaattgcgt gatgaaaaaa
     2041 agaatccttt tattgagctt aaagggcaag ttccagaaga agatatttta gaacaaacta
     2101 aatttctttc gcgcaatggc ttactagaat ccatggtttg ggcggataac actgttcata
     2161 tctgggggag tgtttctctg gaaggggaaa aatatttagt tgaaaactca ggtcttgcta
     2221 aggcgtatgc attggcaaaa gaagttaaaa attggattcc attttggggc taagataagt
     2281 ttttaaacgg agaaggtagc aaatcataat tggtttgttg ccttttttga ttgcccaaaa
     2341 agaaaaatga caaagtatag agataaatga catagtacac gggaaaaatg acattgtatc
     2401 accgacaacg tctaaataga tgttgatata tatacgttcc gtacaaataa ttataaaaat
     2461 ttaaatgatt tgagtagaag tatcagtgaa tatctcaagg ggaatagcta tctcaagatt
     2521 cgattttcac a
//
LOCUS       DARBDV010000171         2495 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000171 DARBDV010000000
VERSION     DARBDV010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.180"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            39..656
                     /locus_tag="SCM56_002863"
     CDS             39..656
                     /locus_tag="SCM56_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111581.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
     gene            1676..>2495
                     /locus_tag="SCM56_002864"
     CDS             1676..>2495
                     /locus_tag="SCM56_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEG1111582.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDF"
BASE COUNT          910 a          334 c          402 g          849 t
ORIGIN      
        1 gcgtttgatt ttcaaacttc gcaacagaac cgagaacctt gggttttcaa gtgctttttg
       61 gttcattaat cttaaccata gtgtttttaa agtggaaaac aagcaacaaa aaaagtatgt
      121 ggagccttgt aggaatagtt cttattattt ttactgtgac tgcagcaaca gaagcaaatt
      181 attcgcataa aattcagtcg tataaaggtc gtttagttat gaccaaaaaa gatgttacac
      241 gttatacatc ctcaagaact ggaggcctat atgactatgc accattagat gcattgaaat
      301 ataaagatca tttaaagaaa catgaagtaa aagttggtga tcggtgggag aatgcatctc
      361 ataaagcata taattccaaa atagtctata cagtagaaga tgccgatgag caaaatatca
      421 cattacctgt attcaattat tggggaacag ttgtgaaagt aaatgggaaa aagagcacta
      481 tagaaaatga caatggttta gtgtctatta aaggtacggg gaaaagaact atagttgagg
      541 tttcatctac gtatccaact acgatgattc tatctttatt aattagttca ataactttta
      601 tattcttatt tacacgttat ttaataagat gctgcggtga aatgaaaata gattagatag
      661 atattataaa tatgttgatt ctattttcat agcaatggag aaaatacatt agcaatatat
      721 aaaagaaggt atcaagagtc acagtgaatt tattccgaaa agtgaaaaga gcaaatataa
      781 aatttaatga aaatatagaa ctaaatagga aaacgtagac gtaattttct acgttttcct
      841 tttcatatat attaaatatt acggaaaatt atataaacta atttagtgtt ttgtttgtaa
      901 ttcctaattt tgctccactt ttagataatt tttaggtcta tttaactcat cagacattct
      961 atgctcattt ttctccaaat ttgtttcaac tctttcgtac ttaattttgc gtatttgaaa
     1021 attcttcttt tagagtactt ttattacgat actagtatga tatatatcta tagcaaacag
     1081 ttaaaatctt cctaaaataa gcgaatagca ttcctaaaac ctataaaatt tttagatgaa
     1141 gcgacaagtg gacaattaat aaactacttg aacatgaaat tatggatcac ctttcaaatt
     1201 tagaaaatgg aattcttata cttattgcgc aacatttatt gattgcaaaa ttatgtgaca
     1261 aaaaaaacta gtagaagaaa gaataatggc ttgataataa agtactacct attaaaggta
     1321 gtactttatt taatttagta taaattaaca tactactata ttttaggttt catattatat
     1381 aaattaataa tatactacca tttgttggta gtataattta aaggtaataa gttattcttt
     1441 tattactatg tttgggtttt atagcaaaga aagtttaata agagtttgga cctagcaaac
     1501 aatattaatg aggtcattaa atcttattat ctttagtgat ataacgaaat atctacacag
     1561 acaaaatgat agcgtaaaat attttgtgta aatagatatt tagggtagaa aaaaagaaag
     1621 tccattctgt aaaattaaag ttaccacaac cgaaatttta taggaggact ttctcatgcc
     1681 caattttact acagaaatta tggaaacact aatcaataaa ggtgatttag atgaattatt
     1741 tcgatgtcat ttagaattag ctgttaattc attattgcag gctgaattaa cagcttttct
     1801 tgattatgaa aaatatgatc gtgctgggtt caattcaggt aattcccgta atgggaatta
     1861 ttcacgttca ttcagaacag agtatggaga attaaatttg gtaattccta gagatagaaa
     1921 tggtaagttt tctcaacaaa ctttacctgc ttataaaaga accaatgact ctttagaaac
     1981 cactattatt cagctatttc aaaaaggaat tactatgtct gaaatctctg aattgattga
     2041 aaaaatgtac gggcatcact atacgccaca aaccatttca aatatgacta aaatagtttc
     2101 tgaagatatt attgccttta aagaaaggtc cttagaatcc agatactctg tcatttttat
     2161 ggatgctact cacattcctt taaaaagaca aactgtttca aaagaagcag tttatattgt
     2221 aattggcatt cgattagatg gaaccaaaga ggttcttggg tttagtcttg ctccaactga
     2281 atctgcttat gtttggaaag agattcttca agacctaaaa gaccgtggtt taaaagaggt
     2341 tttattagtc gtaactgatg gtttaagtgg cattaacgat agtattcata gtgtttatcc
     2401 aaatgctcaa tttcaacaat gttgtgtcca tatatctaga aatatcgctc ataaggttcg
     2461 tgttagtgat cgacaagaaa tctgtagcga tttca
//
LOCUS       DARBDV010000172         2422 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000172 DARBDV010000000
VERSION     DARBDV010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.181"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            119..2272
                     /locus_tag="SCM56_002865"
     CDS             119..2272
                     /locus_tag="SCM56_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEG1111583.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          826 a          425 c          536 g          635 t
ORIGIN      
        1 aagttgattt tatcttgaaa ttaggtaaag aatttcttgc tccgcccatt ttttcttttt
       61 tattaagaag tttttgggta caatgaagca tgaatgtaaa taggcggaag gataggaaat
      121 gatgaaagat tttatgaaaa aaatattaaa tacttcttgg ttagaaaaaa taagaaaagg
      181 tgcacaagaa cctaagaaca aaacgtttcg ccggtcccat gttccttttc gcttaaattt
      241 tctttttttc attatattta cgttatttgt tgcgttgatt gctagattgg gttatttgca
      301 gatcgtgaac ggcgaagaga tggaggcgcg agtgaagtct acttctacga ttacggtcca
      361 agaaagtact cctcgcggaa tgatctacga ctccacagga aaagctttag ttacgaataa
      421 agcgaatcag gccatcacat atacgagagg aacacaagtt acggctaaag atttgcttga
      481 aatcgcaacg aagctgaact cgctgatcga tgttccggta gatgaaaatc tgacagatag
      541 agataaaaaa gatttttggc tggctgatcc caaacatttg aaagaagcca caaaaagact
      601 ttcggcaaaa gaaaagcaat tagggaacag cgaacaatat agtgcgatgg tcaataaagt
      661 cacagctgat gagattaatt taaacgagga gcaattaaaa attgctacca tctttaaacg
      721 gatgaatagt gcatatgaat taaacactgt ctatatcaaa aactctggtg ttactgatca
      781 agagttagct gttgtggcag aacgcgcttc tgaacttcca ggagtttcga ctggcacaga
      841 ctggacacga gaatataatg cagcaagttc actgaaaagt attcttggca gtgtgacgac
      901 tgaaaagcaa ggacttccag cagatgaagc agagaaatac ctagcaaaag gttattctcg
      961 gaatgaccgt gtaggacaaa gttatttgga aaaacaatat gaagatgtcc tgcaaggaac
     1021 caaaacccaa tatgaagttt cccttgataa tgaaggaaat gtttcgaatc aaaaagaaat
     1081 cttctctggg gaaaaaggtt ctaatttaat gttatctatg aatgcagaat tccaatcaaa
     1141 agtagaagaa attttgaaaa ggaattatca aacattgatc aacaacggca aagcacagta
     1201 ttcgcctggt gcttatgcag tggctatgaa tcctcagaca ggagaagtgc tagcaatgac
     1261 tggtttcagt catgaacaag gttccaaaga aatcaccgag aatgctttag gcacgattac
     1321 tagcgccttt gatccagggt ctgtagtcaa ggctggtact ttgacagcag gctgggcaag
     1381 taacgcgatc agcggaaatc aagttttgat tgatgagccg attcgtctgc aaggtgcttc
     1441 agaaaaaagc tctgtcttta atcgaagcgg acaagttgct cttgatgcag tcaaagcatc
     1501 ggaattgtct tctaatacgt atatgatcaa agtggcgtta aagatgttag gactagacta
     1561 tacacctgga atgggactac ctagtttaga tgaagaagct aaagcttatc agcaattgag
     1621 agactctttc aaagagtttg gtcttggtac aacgactggg atcgatttgc cgaatgaatc
     1681 tcctgggatc tcacgttctg ttgattatat gaagaagttc aatgcggata atggcaaaga
     1741 atggtatgct ccaggtaact ttaccgattt ggcatttgga cagtttgaca catatacacc
     1801 aatccaatta gctcaatatg cttcaacagt cgctaatggc gggaaacgag tacagccgca
     1861 cctagtcaaa gcgatctatg gcaatgacga aaatggaaac ttgggtgaag tgaagaaaga
     1921 aattggcaca acagtagaaa atacggtcaa tatttctgca gaaaatatgg cgatcctccg
     1981 tgaaggattc caccaagttg tccatggtac cgatccttac acaacagcca aaccattagc
     2041 cagtgcgaaa atggaccttt ccgcaaaaac agggactgca gaaacggttg cggaaggtca
     2101 tccagatatt gcgacagtca atagtaatat cgtggcatac ggaccaacag acaatccgga
     2161 aattgccatc agtgttgtct tgccgaactt attagacgaa caagaccaca taaacttgac
     2221 gattgctaaa gaaataatgg acgcgtatta tgatatgttc atggcaaaat aaaaaagagg
     2281 tcgtgaaaaa gccgcttagt ttcgagtatg aagaaaaatt ttgtgaaaat agcttctcac
     2341 atttttgctt aaatttgact tcataccgag aaactggctt ttgaacaccg tttactcggc
     2401 aaaaaaggtt gtgacaaaaa tt
//
LOCUS       DARBDV010000173         2408 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000173 DARBDV010000000
VERSION     DARBDV010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.182"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(243..464)
                     /locus_tag="SCM56_002866"
     CDS             complement(243..464)
                     /locus_tag="SCM56_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111584.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(562..807)
                     /locus_tag="SCM56_002867"
     CDS             complement(562..807)
                     /locus_tag="SCM56_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111585.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1047..1661
                     /locus_tag="SCM56_002868"
                     /pseudo
     CDS             1047..1661
                     /locus_tag="SCM56_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2031..>2408)
                     /locus_tag="SCM56_002869"
     CDS             complement(2031..>2408)
                     /locus_tag="SCM56_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HEG1111586.1"
                     /translation="PIEFDNDERLLLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRS
                     YLSDRVFDVLEKLSKSGILKEEYDSSFDEYPSRYILISEDYKKFKRYFLKNEKKISVI
                     MEKYKAPIQADPFSNLDESDLPF"
BASE COUNT          816 a          419 c          332 g          841 t
ORIGIN      
        1 actatatact tcttcccaga acttgtccgt ctccttcaaa ttatctctac ctctcgagtt
       61 cattgggtgg tttgcccatt tattaaacat ggaatcacct ttctcatact catatattta
      121 tgtcctattt caattatatt tatatatgct taaataaatt tgtatcctga aatttattgt
      181 aatatgaagc tgtattttac atatagtaac caaagagtta ataaaataaa aagagcgaac
      241 cattatagat cagctctttt ttcattgctg tttttaatct tatctagaat cacttcaaat
      301 gtttcaaagg tatttacatc taactgacat aaccaaagca agtacttctt ttctttgtaa
      361 gtccaatcaa tattacggta agaggacatt agattatggt accttgctct ttgttcaact
      421 gaagccaatt cccagcgttt ttcaaatatt ttttcttctt ccattattga tccctcgcaa
      481 gacgctacct caaacttaaa taatgggcag cgaaagtcta attccttaga tctaagtata
      541 gcaaacaaat atgaaaaaaa cttagaaggt agtaaaagtt cttccgtctt ttgctttgct
      601 tctattctta atgtgtttcg ttatcggtgc cctacaaaga ttttcattaa ttacacactc
      661 atttattgtt gtgtattttc tgacatttct ctcttgatca attattttaa ttccatgggc
      721 atcctgatag ttcataggag aggagcggct atcagaaata gttgcaccta tctcttgaac
      781 ttcttttaca acttctggaa aatccatcca tcctgcttca gctgtcaaaa gtaataatct
      841 ataatattta tctttccttt tcaatctaag aaatcgtcac cccattcttt ttcttctatt
      901 ataaactatt taacttttag aattcaaaaa ccgtttgtct atttagagtt taccctaaat
      961 agacaaaaat ataacattga aaaatttaga gttctaacgt atgattaggg tgtattataa
     1021 acgttataga aaagtaggga ataaatttga aaattgctta tattagagtt tcatccatcg
     1081 atcaaaatga acaacgtcaa attgaagaaa tgaaaaagtt tggcgctgaa aaaattttta
     1141 tcgaaaaaca atctggagcg acgattagtc atcggccgat tttccaggaa gctttagatt
     1201 ttgtcagaga gcaagatatt tttattgtag aagccattga tcgacttgga cgcaattatg
     1261 acgagattat cgattcagtg aattatctaa agaagaagaa tgtgcgactt attatcacca
     1321 gtttaccgat catggctgaa gcaattggaa atcctctcct agataaattc atcaaagact
     1381 taatcattta aatctcagcc atgattgctg aacaagaacg tactgaatcg aaacgacgcc
     1441 aggcacaagg aattaaaatc gctaaagcaa acggcgtcta taaaggtcgt cctaagttat
     1501 acagtgcaga caccaaagat cctcaacgtc gccttgtcta taggagtatc gttcaggatc
     1561 ttgagaatgg ggttgctatc tcaaagattg caacagacta caatgtgact aggcaaacaa
     1621 tttaccgaat aaaaaaagaa atcgaccaac taattgtatg atttcttttt tgaatcacgt
     1681 aattattaat tagctttgtc atcaatacag acaatcattt tagattcatc tagctaagtt
     1741 gattacattt atgttaaaaa gaatttagtt aacataaata taattgatgc ataaatcgtc
     1801 cttatttaaa ggcatttaac attgacaact tagtataatg tatcttatgt aaactaataa
     1861 gatttgaatt ttattttata cacactcgat aatagatcaa ttttatggtc ccccgcattt
     1921 acgattgcac aagagaaaca taaagtcgac ctattagcat ttcttttcaa ttcaaacatt
     1981 aaaaaagttt atacctctgc tactaaaaaa tgaaattatc tatttaaaat ttaaaacggt
     2041 aaatctgatt catccaaatt ggaaaatgga tcagcctgta ttggagcttt gtatttctcc
     2101 ataataacag atatcttttt ttcatttttt aaaaaatacc ttttaaattt cttatagtcc
     2161 tctgaaatca aaatatagcg tgatggatac tcatcaaaag aagaatcgta ttcttccttt
     2221 aaaattcccg atttacttaa tttttctagt acatcaaata ctctatcact caagtaactc
     2281 cttaaattgt tatcactttc ccattgaact acttttctat aagttgaatt atcagttgaa
     2341 aagatagaat tttcatccat aaacgcatat attagcagca atctttcatc attgtcaaat
     2401 tctattgg
//
LOCUS       DARBDV010000174         2407 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000174 DARBDV010000000
VERSION     DARBDV010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.183"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            5..1150
                     /locus_tag="SCM56_002870"
     CDS             5..1150
                     /locus_tag="SCM56_002870"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEG1111587.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="SCM56_002871"
     CDS             1321..1779
                     /locus_tag="SCM56_002871"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEG1111588.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..>2407
                     /locus_tag="SCM56_002872"
     CDS             1938..>2407
                     /locus_tag="SCM56_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEG1111589.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          802 a          452 c          455 g          698 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttcttta
//
LOCUS       DARBDV010000175         2400 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000175 DARBDV010000000
VERSION     DARBDV010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.184"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            207..2069
                     /locus_tag="SCM56_002873"
     CDS             207..2069
                     /locus_tag="SCM56_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG1111590.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="SCM56_002874"
     CDS             2069..>2400
                     /locus_tag="SCM56_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG1111591.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DARBDV010000176         2303 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000176 DARBDV010000000
VERSION     DARBDV010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.185"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            128..2293
                     /gene="acm"
                     /locus_tag="SCM56_002875"
     CDS             128..2293
                     /gene="acm"
                     /locus_tag="SCM56_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HEG1111592.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
BASE COUNT          906 a          350 c          451 g          596 t
ORIGIN      
        1 tttgtaaaat aactatcaat tttttagtta aaagattgga aatgagtggg ggaggaattg
       61 aagaaaggat ttttgagaga tgatatagta ggttttattt ttagttaaaa taggggagat
      121 agaaaaaatg aaaaagtgta gaaatattat tttatcaatg ttatttatta ttactaatat
      181 aacatcactg attcctgttc atgtctatgc ggatgcaggc agagatatca gcagtaatgt
      241 cacttcgttg accgtggacc caacgaatat tacagatgga ggaaacataa aagtaaaatt
      301 ctcgtttgac gaaaaaaaac aaaatatcca accaggtgat tatctttgga tcaattggcc
      361 aagtgaagga aatattcgcg gtgaaggatt ccaaaaagaa atcccattaa tgattgaaaa
      421 taaaaatgtt ggaactttaa cggtcagaaa agattctgcg caggttgtgt ttaatgaaaa
      481 tattaaaaac ctagactcag tagaaggttg ggggcaattt gagatacagg ccagaaacgt
      541 aaccgatacc ggtgaagaaa atacgggacc attcaccgtg acgagcggtg ataaaacagc
      601 tactgtaaat gtgacaaagc cagcttctgg ttcttcttct agcgtttttt attataaaac
      661 aggagacatg ctacctgaag atactaagca cattcgatgg tttttgaata ttaacaataa
      721 cggcacttat gttgaacaac cagttaaaat cagtgatgaa atccaaagtg gacaaagatt
      781 agatccaagt acgtttgaaa ttaatcaaat acacttaggt gaacagaagg tttatagagg
      841 agaagaggga atccaacaat ttttacaaga tttccccagc gctacattta atttttcagt
      901 aactgataat tacattgaaa taactatacc aaaaaacttt gtcaatctta ggaaaataat
      961 ggtgagttat aagacgatca tagaaaaccc agaacaaata aattttgaga atcattcgga
     1021 agcttggttt aaagaattta ataaacctgc tgttgatgga gaaagtttca atcatacagt
     1081 taaaaatatt tcagcttctg gtggagtcaa cggtacagtt agaggcgaat tgaagatttt
     1141 caaatatatc aatgacacag agattggtat cccaaatgtg actttcgaat taagacgagc
     1201 agatgaacag cctattcaag gacagtctag tatcctgttg acttcgaatg aacagggaga
     1261 agctagtatc aaaggcctgc aagtaggaga ctatgttgta aaagaaaaag aagcacccaa
     1321 ttggattgat tttgatccac taagttcaaa tgaactcaaa ttctccataa acgaaaatga
     1381 tacagaggga gtcagtttac ctatttataa caagaaaaag gttacgaaca tcacagcaac
     1441 taaaatttgg aacggtggaa ccactccaag accgagtatt tattttaaat tattcagagc
     1501 aagtacaaac aattgggaac cagttcctga tgcagaaaca aaacgattag acaatggaat
     1561 aacttcagtg acttgggaag atatccaaca gtatgatgat agtggaaacg aatatacgtt
     1621 taaagtgcaa gaagtagatc aaaacggaaa tgattatgtc ccttcaggtt atcaaaaaat
     1681 tgaaaacggg ctagtcgtta caaatgagaa taaagaagtc atttcagtct caggacaaaa
     1741 aacatgggaa gacaatgaga accaagacgg caaacgaccg acgacgatca cagtaaatct
     1801 attagccgac ggtcaaccga tccaacacaa agaagtaagt gaaaaagatg attggcgcta
     1861 tcgtttcaca aatctgccaa aatacagaga cgggcaagaa atcatctata ctgtaacgga
     1921 agacaacgtg cccgaataca gtacgacgat tgacgggtat aacatcaaaa acagttatac
     1981 accggaaaaa acaaatatat ctataccagg tagccatata acaccgtgga aaaattttcc
     2041 agagaagaca gagagagaga aaattagaaa tctattctct caaaaattgc aaatatttgc
     2101 tcatacagga gaatccagcg aacaaagaag caattatatt agtaagagag ctgtacctaa
     2161 acagataaca aataaatcaa aaagcattct tcctaaaaca agtagtaaaa aatctagttt
     2221 tgctgtaatc attggtttat tgatagtaac tatctgtgct gggatattct tacaaaaata
     2281 taaaaaagtt tagttagaat ata
//
LOCUS       DARBDV010000177         2282 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000177 DARBDV010000000
VERSION     DARBDV010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.186"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..777
                     /locus_tag="SCM56_002876"
     CDS             <1..777
                     /locus_tag="SCM56_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG1111593.1"
                     /translation="KSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSA
                     RTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHL
                     EGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFKSIT
                     FDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSVDE
                     FFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT          829 a          300 c          461 g          692 t
ORIGIN      
        1 aaatcaaact gtggtagacg tccgcttgtt ttacccgagg aacaatcaga atatattcaa
       61 agaaaggttg ttcaaggatg gacacccgat gtgattgttg gtcgtgcagc gtttcctatt
      121 agttgttctg ctcgtaccat ttatcgtatg ttcaaaaagg ggctatttga ttcttctgac
      181 ttaccgatga aaggcaagcg taaaccgaat ggacatcaag aaagacgtgg aaaacaaact
      241 ttccgccgct ctattcatga acgtgaaaag gattatagcc aattctcaaa tgagtttggt
      301 caccttgaag gtgacactat cgtaggtctg aaacataaaa gtgctgtaat taccttagtt
      361 gaacgattat caaaagttat catcacattg aaaccgtgtg gtagacaagc gattgatatt
      421 gaaaaaaaat taaatcaatg gtttgaatct gtaccgaaaa acctattcaa atccatcact
      481 tttgattgtg gaaaggaatt ttcaaattgg aaacagatca gtaatgtcaa tgatattgcc
      541 atttatttcg ctgatccagg aacgccgtct caaagaggcc taaacgagaa ttctaacgga
      601 ttgttacgta gagatggttt attgaaatct atggatttca attcagtaga tgaatttttt
      661 attcaatctg tcgcatctaa acgaaataat attcctagaa aatcactgga ttatcgaaca
      721 cctttggaag tatttttgag ttacgtaagt attgatgatc tgtctaattt aatttgacaa
      781 ttaagaaagt attaacttgt tttgtgaaaa agagaatttg aaaaacgatt ggcgaacaaa
      841 tgctcggtag gtatggtata aatacagagc taattggtgt gatgaaatca gatattcgcg
      901 tgagattcag taaagtagga gttcgaaaac agctaggtga caaacagtat tgctttggta
      961 cttcggaatg gtttatcaaa ttcttatcaa cagttcactt ctctttatgt ttgagtgatg
     1021 attcgttagg ttattgcttg gaaagaccgg atgagagtag aagagtttaa ttttataaag
     1081 tgaaaagagg taatgtacga tatacgtagg gctgaaccaa tagaggataa gcaaacgatg
     1141 ttacaggcgc aaaattcata gcttactgtt ttggagataa cagaagagga aataatgcag
     1201 aagtaaggcg aaaaaaggac ggaagcctaa cagcatatga aataaagaaa aatattataa
     1261 ctactgataa atcggtattg agcaaagtac taataggagt caaaaacatt cataagctat
     1321 gagtgtttct tggctacttt tttattttta tgtatctaca aaagtttcca ggggcagaac
     1381 ctgtgattat taatggaaga acctcaagta ttgtcttaaa tcattgttat gaaaatggat
     1441 taaaagagtt catcaaggtg tgattaacaa gttacttttc attacatcga atttttcaga
     1501 agttgcttat attggtgatg atattaatga tttatcatgt atgacatctg tgaagaagcg
     1561 ggaggaatag tagaatgtcc aaaaaatgcc acaagaaatg agattgaatt atctgatttt
     1621 attgtaactc acagtggtgg tgacgaatct gtgatagagt ttattgagtg gatattggaa
     1681 tcgatatagt gtagtagtac agaaaaatta tcatacaatt aatactaggc ataagaatat
     1741 tggcatattc tagagttaaa agtaactatt cgaaaaggat aaggttagaa ttttttgttt
     1801 tgccagtgga agaatataaa aagggcattg gaaattaata tattagaatg ctaagcggag
     1861 aaaaaaatat atgaaaagta cgtccttgtg atgggatgga aaaatacact tatttcaaac
     1921 aacgataata taattatgtg aaccgaagta tactcgcttc taattcaatc ctcaaatccc
     1981 gacagtcgcg acttcaaaaa agcgtatagt tttaatgtat ccccatatga tccgaaaaat
     2041 ggtaatttac aattaacagt ttgatgatgt tgaagatgtg gaaatgctga aattaccatg
     2101 atgagaaaca cattaattaa agggaattaa ttaactagaa tataatctga ttcaatttga
     2161 attaagcaaa aaaaggctga gttttaaata atcgcgaatt gtaaaattaa gttagaaaaa
     2221 taaaaaggca tttatggtac actcaaattg tatttccgac gaaagaaaac aaaggagtgt
     2281 aa
//
LOCUS       DARBDV010000178         2065 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000178 DARBDV010000000
VERSION     DARBDV010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.187"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1008
                     /locus_tag="SCM56_002877"
     CDS             <1..1008
                     /locus_tag="SCM56_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111594.1"
                     /translation="LLDAPYYLSKVALGENLIKPESQQPVTNSNYLINTYNIKPMVKG
                     KKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTDVWSGTFTAAADPHPTS
                     PQVQICQVPSTSLGNCTIKWLKLEEGDTRTPNIEQYKYRGIGMRDSNNPKDYVWDLAP
                     EYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTKANANAT
                     AIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALNGRSSGGNDST
                     ILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAITYT
                     I"
     gene            1025..1174
                     /locus_tag="SCM56_002878"
     CDS             1025..1174
                     /locus_tag="SCM56_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111595.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1171..1617
                     /locus_tag="SCM56_002879"
     CDS             1171..1617
                     /locus_tag="SCM56_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111596.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1619..1756
                     /locus_tag="SCM56_002880"
     CDS             1619..1756
                     /locus_tag="SCM56_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HEG1111597.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            1794..>2065
                     /locus_tag="SCM56_002881"
     CDS             1794..>2065
                     /locus_tag="SCM56_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111598.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKT"
BASE COUNT          753 a          358 c          425 g          529 t
ORIGIN      
        1 ctacttgatg caccgtatta tttgagtaag gtggctttgg gtgagaatct aattaaacca
       61 gaatcacaac aaccagttac taatagtaac tatcttatta acacctataa cattaaacca
      121 atggtaaaag gtaagaagta taccatcaca cttgaaggaa ctaagccagc aacacaggtt
      181 tttagaccat ttttcacacg agcaacagga gatgcatggg aggttggtga cttacaacca
      241 gtagaagggt taactgatgt ttggtctgga acatttacag cagcagctga cccacaccct
      301 accagcccac aagttcagat ttgtcaggta ccaagcacaa gtttaggaaa ctgtacaatt
      361 aaatggttaa aactagagga aggcgacaca cgaaccccga atattgagca atataaatac
      421 cgaggaatcg gcatgcgaga ctcaaacaat ccaaaagatt acgtctggga tctagcacca
      481 gaatatgtcg aagacaatct ggccacagat attaaaattt ctgaaattac tggtaaagca
      541 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
      601 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
      661 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
      721 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
      781 tattttgtgg ctttaaacgg aagaagcagt ggtggaaatg attcaacgat actaacaatt
      841 ccagaaggct atcggccacc aattagtttt gagcaactgg ttggctcgat agaccgttct
      901 actttgaaca gtgctcagtt atctattggt gcagatggag ccattaaatg gcgaagaaac
      961 tcaagttatg gatcggatta tacctttgca attacttaca cgatttagaa aagcgtgaat
     1021 cgatatgaag gcagcatata gaccaattga accttacgga ttcgagcaaa tcattgtgaa
     1081 tgatgaagaa catttaccgg aagaatgcac agaagtcgaa ccaccgattc caaattggaa
     1141 accgaaattc aattactggg agggaaataa atgaaaaaca tttggaaata tggacgtact
     1201 ggcggagagt acgcaggaaa agtattggac gacatgcttg tatccgttcc ttacacagat
     1261 cagccaccgc ttgaaggaat tcgtgctgat ggcgaaccgt taacaatcgc tgatcaaatg
     1321 tttgatccta aattgaatca atggattatt ttagcgaacg cactagatca caacgattta
     1381 aacaatctca aagcaatgta tgagtcgtta gaaaatgaga acggcgattt aaaacagatc
     1441 aatgccaaac tcatgctaag cgatgtagca attaaacagg aaaatactgc attgaaagaa
     1501 aaagcggata gtttagcaca aatcaattca aaaatgatgc ttgcttcgtt acaaaatagc
     1561 aaagacattt cagaaattaa agagcaacta aatccagctt caaagggagg tgagtagtat
     1621 gtttagtttt agcgatgtga aaatgatgta tgattggggc tgtttcacta acgaacaagt
     1681 aatggttttc gttccgttgt gcattactga agaaaaagca gataaaatca ttagcaaaga
     1741 agagagcgca tcttaattga tgtgcttttt attttgattc aaggagttgt cacatgatta
     1801 atttagggga atggggagcg atagcaggat caataaccgc tatcgtttct ttgattttat
     1861 tagtaataaa accaattact gcatctttct cgaagattac tgagactctt tcaaaagtaa
     1921 gtcacaattt agatttgctg actaaagatt tagaatcgag caaatcagat cgattgatga
     1981 ttcatgaaga actaaagaaa cacgatgaaa gattagatac acatgcagaa aaattggtag
     2041 aacacacgca acaaattaaa acttt
//
LOCUS       DARBDV010000179         2040 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000179 DARBDV010000000
VERSION     DARBDV010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.188"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1872
                     /locus_tag="SCM56_002882"
     CDS             <1..1872
                     /locus_tag="SCM56_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEG1111599.1"
                     /translation="SAENMEEIKKMFPKSTWFGFTGTPIFPENRKQAKGQLARTTHDQ
                     YGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENKIYRKLKEVETYAEYSSEQIN
                     RMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTT
                     SSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTMEDPDFPRIAITYSLEENSRD
                     SSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNRLARKRAEFKQFGKQIDLAIV
                     VDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPDKTKGLIVTFRKPATMEKN
                     VEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKEVSSMESIDEHTPLEIRIEYV
                     KAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEEQIGVYETVKGSLIEEEPEKE
                     GEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDAREEIEKALAKLNKTEGVKE
                     VYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHVFSNEWFVSEKELHASNLQYL
                     PGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          738 a          330 c          416 g          556 t
ORIGIN      
        1 agtgctgaaa atatggaaga aatcaaaaaa atgtttccga aatccacttg gtttggcttt
       61 acaggtacac cgatttttcc tgagaatcgg aaacaagcca aagggcaatt ggcacgtaca
      121 acccatgatc agtatggaga ggtcttgcat acctatacca ttaagaatgc gctagaagat
      181 ggctctgtct taggatttca agtagagcat gaaaacaccg tggagccgac ttcactagag
      241 aataaaatct atcggaaatt aaaagaagta gaaacatatg cagagtattc atcagaacag
      301 atcaatcgta tgattgatca aatggaacct gtcaaaaaag aaagttattt ggacccagct
      361 gtttttgaag cagatgaaca tatccaaaaa gtcattcata aaatgttccg tccagataat
      421 gcctatacaa aatttgattt tcgaaatggc aggccaacga aatctgcaat cttaaccacg
      481 agttccattg acatggccaa aaagtattat cgggccatca aagagatgac gaaagagcct
      541 gattggttaa caaaagagtt tcccaatcag ctcattcgtg aaggacgaac gatggaggat
      601 cctgattttc cacgaatcgc tattacctat tcattagaag aaaatagccg agattcaagt
      661 gcccaacaag aagaaatgca aaaaattatt gaggaatata atcagtatta tgatacttcc
      721 tggtcattag cagacattga gcgttataac ggagacatca ataatcgttt ggcacgaaaa
      781 agagcggaat tcaaacaatt tgggaaacaa atcgatttag cgattgtagt ggatcgtcta
      841 ttaacaggtt ttgacgcccc tacgattcaa accctatttg tggatcgtaa tctagagtat
      901 gcaggattga tccaagcttt ttcacgtacg aatcgtacgt atcctgataa gacaaaagga
      961 ttgattgtga cgtttcgtaa gccagcgaca atggagaaga atgtcgagga tgcgacaaaa
     1021 ctgtattcaa aagcaaaaga agaatcaggt ttgatttatc caagttatca ggaatccaaa
     1081 aaaagattca aacaagcata tggtaagtta aaagaggttt catccatgga atccattgat
     1141 gaacatacgc cacttgaaat ccgtatcgag tacgtcaaag catttcaaga attgaatcgt
     1201 gcttatgaat cgttagtgac ttatgacgat tacaatgatg acatggtgaa atctccaact
     1261 ttgaacaatc aaattcaact tttggaagaa caaattggcg tgtacgagac cgtgaagggt
     1321 tcattaatag aggaagaacc agaaaaagaa ggagaagatt tttcaacgat cgaattttat
     1381 agtgaaaata ccactaagtt gtatgatatt gattcaactt atattgatca attactagga
     1441 acttatgctg cgaatagttc agatgcacga gaagaaattg aaaaagcttt ggctaaattg
     1501 aataaaacag aaggagtcaa agaagtttac cgacaaattt taaatgcatt tgatgtgggg
     1561 actttagata gtaatgaaga tatctttgtc attaaacgtc gctattttac acaagcgagt
     1621 gatgatttga ttcatgtctt ttccaatgag tggtttgttt ctgagaaaga attacatgct
     1681 tctaatctgc aatatctacc aggtgaagat ccaattccta atatgaaagc aattatcaac
     1741 agtaaggatt acgaaggata taaagccaaa catccagaag cgaagccatt taaataccca
     1801 caagaaatga agcgagcatg gcgtaaaatg ttagatgatg agttgattcc acttgagaat
     1861 gaattgaggt aaacgagaag agttaagaaa attgtacgat tcaaagatgg tagtcaagtg
     1921 gaagtgtaga aaatagggat agatgtcttt tatttgttca cattttataa cgaagtattt
     1981 aagacagcta ttgaataatg tttcctccac ctttttcatt catttaccgg ttctgttgca
//
LOCUS       DARBDV010000180         2003 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000180 DARBDV010000000
VERSION     DARBDV010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.189"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..378
                     /locus_tag="SCM56_002883"
     CDS             <1..378
                     /locus_tag="SCM56_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEG1111600.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="SCM56_002884"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="SCM56_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HEG1111601.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2003
                     /locus_tag="SCM56_002885"
     CDS             1859..>2003
                     /locus_tag="SCM56_002885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111602.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DARBDV010000181         2002 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000181 DARBDV010000000
VERSION     DARBDV010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.190"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..545
                     /locus_tag="SCM56_002886"
     CDS             <1..545
                     /locus_tag="SCM56_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111603.1"
                     /translation="KKIQIRALVCTICATVISLVFIYIIDTGILALLMSNLGITDDGA
                     RLSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLNGGRIAASLHSDILTIYI
                     EVGCIVFIIWMFWLFFVKPIRLTKKFGLKTGECVLLLTIYMFILYLTDNTLTYPDTQM
                     IFLLVPMIVSWVDNQQRISE"
     gene            629..1717
                     /locus_tag="SCM56_002887"
     CDS             629..1717
                     /locus_tag="SCM56_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111604.1"
                     /translation="MKKILVLANTYYQIIVAIQMKNTIFSDEYVEIIISDHSKNTKEI
                     AKKIEEKKVFDGCHYLESKSFDDNIGYFQKIKDCLQISCGKSNRYAYYLKNCKSEYFD
                     EFICFSMGEVWLEGIYACLYRNNKNVQVSILEEGILSYGVDIIPTARTKAIAVLNYLK
                     KRTPLYNKSYNFYCFFPNIYTGRENTIQIPLVTAGSRTALQLSDIFNPMIDIYKEKKY
                     IFFTSVYDFEGGEPVGEYELVCKIADLVGKDNLIVKTHPRDVRTIYKDNGFIEDKNSS
                     IPWEAIQLSGDFNDKIFMTINSGSVISGSTMSAKPVRTYYMYKLCNIEGNPSCKKNAY
                     DIERLLKQKTMKNILKNVKIAESLDDIL"
     gene            1714..>2002
                     /locus_tag="SCM56_002888"
     CDS             1714..>2002
                     /locus_tag="SCM56_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEG1111605.1"
                     /translation="MSKTKYLIKNMGILTISNFSSKILVFLLVPLYTSVLSTTEYGTY
                     DLTISTATLLFPILTLNIVDAVMRFLMDKDKNKKNIASIAIKVIFLSVIVFG"
BASE COUNT          742 a          222 c          344 g          694 t
ORIGIN      
        1 gaaaaaagat tcaaatccgt gcattagtgt gtactatttg tgcaactgtt atttcgttag
       61 tgtttattta cattatagat acaggaattt tggcattgct tatgtcaaat ttgggcatta
      121 cagatgacgg tgcgaggctt tcattttatc tatattcatc taaattttat gaacttggtt
      181 taggatatgt aggaacagga tggacatggt tttcaaaata cttttttaat ctttatttat
      241 cgcactttag gttaaatggt ggaagaattg cagcaagcct acacagtgat atattaacta
      301 tttatataga agttggatgt atagttttta ttatatggat gttttggtta ttttttgtga
      361 aaccgataag actaactaaa aaatttggat tgaaaacagg ggagtgtgta ttattattaa
      421 ctatatatat gtttatatta tatttgacgg ataatacgct cacttatcca gacactcaaa
      481 tgatttttct cttagtacct atgatagttt cgtgggttga taatcaacaa cgaatatcgg
      541 aatagatagc taaagtatat tttaattatg aatttataat tagttgtaaa agttaatatt
      601 taacggacag gaagtagggt gaaaaaattt gaaaaaaatt ttagtgttag caaatacata
      661 ttatcaaata atagtagcaa tacaaatgaa aaatacaatt ttttccgatg agtatgttga
      721 aattattatt tctgatcatt ctaaaaacac taaagaaatt gcgaaaaaaa tagaagaaaa
      781 aaaagtgttt gatggctgtc attatttaga aagcaaatct tttgatgata atattgggta
      841 ttttcaaaag attaaggact gtcttcagat ttcttgtggt aaatcaaatc gatatgccta
      901 ttatttgaaa aactgtaaaa gtgaatattt tgatgaattt atttgttttt caatgggaga
      961 ggtttggttg gaaggaatat atgcttgctt atatcgaaat aataaaaatg tacaggtatc
     1021 aattttagaa gaaggtattt taagctatgg agttgacatt attccaacag caagaacaaa
     1081 agctatcgca gtactcaatt acttaaaaaa aaggactcca ctatataaca aaagctataa
     1141 tttttattgt ttttttccta atatatatac aggaagagag aatacaatac aaattccatt
     1201 agttacagct gggtcaagaa ccgcattgca actttcggat atttttaacc ctatgataga
     1261 tatttataaa gaaaaaaaat atatattttt tacgtctgtt tatgattttg agggcggaga
     1321 acctgtcgga gaatatgaat tagtatgcaa aatagcagac ttagtaggaa aagataatct
     1381 aattgttaag acgcatccta gagatgtaag aacaatttat aaagataatg gatttatcga
     1441 ggataaaaat tcatcaatac cttgggaggc gattcaattg tcaggcgatt ttaacgataa
     1501 aatatttatg acaattaatt caggaagtgt tatttcaggt agtacaatgt ctgcaaaacc
     1561 tgtacgcacg tattatatgt ataaattgtg taacatagaa ggaaatccgt cttgcaaaaa
     1621 gaatgcgtat gatattgagc gattgcttaa gcaaaaaacg atgaaaaaca ttttaaagaa
     1681 tgtgaaaata gcagaaagct tagatgatat actatgagca aaactaaata tttaattaaa
     1741 aatatgggaa tattaacaat aagtaatttt tcttcaaaaa ttttagtttt tttgttagtc
     1801 cctttatata caagtgtatt atccacaaca gaatatggca catatgattt gacaatttca
     1861 acagccacat tattgtttcc aattttaacc ttaaacattg tagatgctgt gatgcgcttt
     1921 ttaatggata aagataaaaa taaaaaaaat atagcatcta tagcgattaa agttatattt
     1981 ttaagcgtga tagtctttgg at
//
LOCUS       DARBDV010000182         1991 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000182 DARBDV010000000
VERSION     DARBDV010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.191"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="SCM56_002889"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="SCM56_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEG1111606.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="SCM56_002890"
     CDS             852..1040
                     /locus_tag="SCM56_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEG1111607.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="SCM56_002891"
     CDS             1053..1610
                     /locus_tag="SCM56_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEG1111608.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="SCM56_002892"
     CDS             1642..1884
                     /locus_tag="SCM56_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEG1111609.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          724 a          308 c          406 g          553 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1981 ttctttattc c
//
LOCUS       DARBDV010000183         1962 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000183 DARBDV010000000
VERSION     DARBDV010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.192"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..272)
                     /locus_tag="SCM56_002893"
     CDS             complement(<1..272)
                     /locus_tag="SCM56_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111610.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
     gene            complement(309..446)
                     /locus_tag="SCM56_002894"
     CDS             complement(309..446)
                     /locus_tag="SCM56_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HEG1111611.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            complement(448..894)
                     /locus_tag="SCM56_002895"
     CDS             complement(448..894)
                     /locus_tag="SCM56_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111612.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            complement(891..1040)
                     /locus_tag="SCM56_002896"
     CDS             complement(891..1040)
                     /locus_tag="SCM56_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111613.1"
                     /translation="MKVVYKSIKPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            complement(1054..1386)
                     /locus_tag="SCM56_002897"
                     /pseudo
     CDS             complement(1054..1386)
                     /locus_tag="SCM56_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2479 domain-containing protein"
BASE COUNT          509 a          407 c          342 g          704 t
ORIGIN      
        1 aaagttttga tttgttgcgt gtgttccacc aatttttctg tatgtgtatc taatctttca
       61 tcgtgtttct ttagttcttc atgaatcatc aatcgatctg atttgctcga ttctaaatct
      121 ttagtcagca aatctaaatt gtggcttact tttgaaagag tctcagtaat tttcgagaaa
      181 gatgcagcaa tcggtctaat tactaataaa atcaaagaaa cgatagcggt tattgatcct
      241 gctatcattc cccattcccc taaattaatc atgtgacaac tcctttacct taaataaaaa
      301 cgcatcaatt aagatgcgct cttatcttta ttaatgattt tatctgcttc ttcgtctgta
      361 atgcatagtg gaacgaatag tcgaacttga tcgtcagtaa aacagcccca atcatacatc
      421 attttcacat cgctaaaact aaacatacta ctcacctcct tttgaagctg gatttagttg
      481 ctctttaatt tctgaaatgt ctttgctatt ttgtaacgaa gcaagcatca tttttgaatt
      541 gatttgtgct aaactgtcag ctttttcttt caatgcagta ttttcctgtt taatcgctac
      601 atcgcttagc atgagtttgg catttagctg ttttaggttg ccgttttcat gttcaagagc
      661 ctcgtacatc gctttgagat tgtttaaatc gttgtgatct aacgcgttcg ctaaaataat
      721 ccattggttc aatttaggat caaacatctg atcagcaatc gttagcggtt cgccatctga
      781 acgaacccct tcgagcggtg gctgatctgt gtaaggaacg gatacaagca tgtcgtccaa
      841 tacttttcct gcatactctc cgccagtacg gccatatttc caaatgtttt tcatttattt
      901 ccctcccagt aattgaattt cggtttccaa tttggaatcg gtggttcgac ttctgtgcat
      961 tcttccggta aatgttcttc gtcattcaca atgatttgct cgaatccgta aggcttgatt
     1021 gatttgtaaa ctactttcat tagatttcct cccttaaatt gaataagtga taacaaatga
     1081 ataagctgat ccataacttg agtttcttcg ccatttaatg gctccatctg caccaataga
     1141 taactgagca ctgttcaaag tagaacggtc tatcgagcca accagttgct caaaactaat
     1201 tggtggccga tagccttctg gaattgttag tatcgttgaa tcatttccac cactgctttt
     1261 cccattcaac gcaaaaaaat aaatagtgac ggtttttcct tcacgataaa gctttgctga
     1321 tccggtattc ccgtttgtaa ctgttaaggt tacgacttca tatttgttgt cgtctgatgt
     1381 caatacagat tttccttttg attgaatacc atcttcgaaa tttttcaatc cttcgatgga
     1441 ttgcggttca gtcaaactaa ccgtatcatt caagcctttt tcagtatatt caggcgtgat
     1501 gtcccagctg tagtcgttcg gattgttgct atctttcaat ccttcaccaa agtatttaaa
     1561 ctgactaata ttcggggttc gggtgtcgcc tttttcaatt ttgagccagt caatttggca
     1621 tgcgccggcc gtttctttag gcaactgaaa aatgcgaagg tctttaggca aacccggttc
     1681 aagtttcgtt ggtgtgaatg ttagagacca tacgtctgtc aatccctcaa ctggttttag
     1741 ttctccaaaa ttaacgttcc aataattata tgctacaaag gtttgacttg cgggttttgt
     1801 tcctttaagc gtgatagtat acgtttgacc tattataagc tcttcttccg tgttaccttt
     1861 atatatttcg tacgcgctag atttaattgg gaacgtcgta cctttatttg caatattctc
     1921 acccaacgat gttttaccta cccagtaagg gtcatctagt aa
//
LOCUS       DARBDV010000184         1956 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000184 DARBDV010000000
VERSION     DARBDV010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.193"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(1..635)
                     /locus_tag="SCM56_002898"
                     /pseudo
     CDS             complement(1..635)
                     /locus_tag="SCM56_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            1120..>1956
                     /locus_tag="SCM56_002899"
     CDS             1120..>1956
                     /locus_tag="SCM56_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111614.1"
                     /translation="MRWYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAF
                     HINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLY
                     IVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPL
                     ILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIF
                     LIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDS
                     ELL"
BASE COUNT          619 a          301 c          314 g          722 t
ORIGIN      
        1 aaggtaaaaa ataagtctga ttccttgtct ctaaaggctt cggtcagctc atggaaaaaa
       61 gtataggcgt cctttaaagg cgttgaaaat gccagcaatc gatcaatcat cattgcttca
      121 gtgagtagcg ggtatttagg tgcacgaaaa ctgcgccatg ttttgtaatc atagtgattg
      181 attttttgac ggttttttag aagaaagcgc cagttctttt tcactttctc ggcttcccgt
      241 cgttgcccta actggtgtaa tcttttcatt tcttgtacac gaaaatcatt gaacgcctga
      301 ttcaaatgtt tgatgatatg aaaacaatca atgacgactt tcgcatttgg taagacacgt
      361 tttgtcagtt ggaagtaggc ggcgttcata tctgtcacta aatattccac cgcttcagga
      421 tttgcacagc gactaaaata gtgtgttaat cgagacaatt ttcgtgtagg caaaatatca
      481 atgagttttc cagtctcgcc atccgcacaa ataaagctca ttttatccac aattgaagcg
      541 tgcgaacgaa attcatccac catcaatact tttggcaggg tcctttttga cggcttcgga
      601 agatagtttt tcaactcttt caatgtacga atcatatacc taaaataaca atgggactga
      661 agaatcaatt ttgattcatc agtcccataa attatagagc ctatattttg cgaattgcgc
      721 tacggtttct tcttttcttt tgtctgtaat atgaatatat agatccataa tgatttgaat
      781 agaagagtgg cctaaacgct cttgcacatc tttaatattt gcactagctt gctaaaagca
      841 agctagcatg tgtgtgtcta agaccatgaa tagtaattcg tttaagattg tttttcctga
      901 tgattacatc taaccatttt cgagatttag ataattgaag gtgttcattt ctttctggta
      961 aaaatttaaa tatataacta cattttctct actaactttg aaacactact tttgtaatat
     1021 aaaaaatatt ttattaataa taaaattaat cacattttat atagtgagta atctagtgtt
     1081 ttaaaaaaat aaatgatact atatgtaata taaaaaaaca tgaggtggta tatgaatttg
     1141 aaaaaaattt tttctgtaaa aatatttata gtttatgcat ttgttagttt attatattta
     1201 tttccagcat ttaaaaacat ggaaatattt ggcttttatg atacggcatt tcacattaat
     1261 cgtgcgttaa gtttagaatc tatttttagc tcccctatta attttgaaac gtttagatca
     1321 tatggcatgc aagtaaataa tttttaccca tggttaactc tatatccttt atttttattg
     1381 atcaagttta ctaatttagc aataggctat aacctttttc tttatattgt tacgctcatt
     1441 actctattta tatgtcatta tgtaatgtat gaaattacca aaaaacatgt tactagctcc
     1501 tttttcgcta ttatatatac aacatcttct tttaggagtg tagaaatatt tcttcgaggg
     1561 gcaatggggg aattactcgc aatgtcaata cttcctctta ttttactcgg gtttataaag
     1621 ctttacgact caaaaaaaga atcttgggtt atgttggcga ttagcatgac tttattaatt
     1681 tacactcatg ttctttctgt agctatgact atggtaatga ttataattgc tttaattatt
     1741 caattttatc ggaaaaagaa aatagaaatc ttcttaatta ttaaattaat aaaagctgct
     1801 atagttactt tacttctttc actagcgttt ataggtccta tgattgagca gacattgtat
     1861 caagatctga atagacctta tgtggatata ttacaaaaaa gagcaattta tctgggtaaa
     1921 gagtttttga ttggaagtat agattcagaa ttacta
//
LOCUS       DARBDV010000185         1952 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000185 DARBDV010000000
VERSION     DARBDV010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.195"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..402)
                     /locus_tag="SCM56_002900"
     CDS             complement(<1..402)
                     /locus_tag="SCM56_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEG1111615.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQK"
     gene            complement(399..1052)
                     /locus_tag="SCM56_002901"
     CDS             complement(399..1052)
                     /locus_tag="SCM56_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1111616.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1340..>1952
                     /locus_tag="SCM56_002902"
     CDS             1340..>1952
                     /locus_tag="SCM56_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1111617.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFL"
BASE COUNT          615 a          346 c          251 g          740 t
ORIGIN      
        1 tttttgcata tcagctgtag aaacaaattg tagatttgtt tttttaccat cattaagctt
       61 tatttctctt tcttcaatta tcaaagaatt actatcactt tctaaattaa catttatatt
      121 atctttgatt tgtatttcat tcttattatt tttttctttt atgaatactt ttatttcact
      181 actcttggaa taaaactcca aagtttgttc tatatatttt atttcttttc cattcatatt
      241 actagaaatt tcatttgcca tattatgaat cttttctttt ctcgtctcca aatatgtttt
      301 aggaaagata aaaaaaatta atgaatgaac taatataatt attattccaa gaacagaaaa
      361 tatttgtatg aacatttttg gaaatatctt taattttttc attttctctc caatttatat
      421 cctacatttc ttatagtagt tatacagtct aattgtaatt tttttctaag ttcttttata
      481 tatacatcta ttactcgatc ataaggagtt tcttcgctat ctttccatac ataatcaata
      541 atttgcattc ttgttaacac ttgtccatta ttatccaata aacattttaa tacttctagt
      601 tcttttgcat ttacatctat ttcttcacca tttattttag ctgtatagct attaaaatta
      661 accgatagat ccttatattc aaacttctct aaatgtccat aattcttctt aattaaagaa
      721 tctattctag cttttaagac cggcaatgaa aatggctttt ctacatatcc gtctactaaa
      781 ttagtaaatg catcaatttt atattcttca tcactaaatg cagtcaagat tagaattggt
      841 aaattgcttt tctttctaat ttctttcaac acttctaaac catttataaa aggtatctga
      901 atgtctaaga taaccaaatt tatatcgcta ttaaattttg acaaggcttc tcttccatct
      961 ttagcttgaa taacagtata tccaaattct gaaagatatt cacttattcc ctctcttatc
     1021 atattatcat cttcaacagt taatattttc atatatacct ccacttaagc ctatacacta
     1081 gatattatac agcatattat ggctctataa tatctagtgt tggtgacacc ttctgttgcc
     1141 aatacttttt cttttttcta ataaatgcaa acagacacct tgaaatcctt tagaataaag
     1201 ggttctataa tattttgtgt tgttggaaaa tcgaaggcga ttttctacta acactttttc
     1261 ttcgtttcaa ctcaaaaaga gactcacttt tggtaaaatt tagtcgacca aaacatcacc
     1321 ctaccaaagg agtcgctcta tggataatca tactagaaaa ttactcggat taacagataa
     1381 acatttattt tttgatgaag aatggctgat tgaaaaagaa tataaaggcg ttcaagcgca
     1441 gtttattaaa gggagattgg atgactcacc ttcccattgt gaacactgtg gatcgattcg
     1501 tattatccgc aacggttcct atacgacacg tactcagata ttgaaagtca aagaaaagtt
     1561 gactatttta gaattaaaac gtacccgttt tctatgctat gattgtgggc agaccttttc
     1621 tgcgaaaacg gacttagttg atgaacatca tcaactaaca aaggaactga agcaggcgat
     1681 tctcatagaa ctgtacgaaa atcaatcacg taaactaatc gctaagaagt acttcgtctc
     1741 ggatgggacg gtgacacgga tcttgcgtga agcaacgaag cattaccagc cacgtatgaa
     1801 cttccttccg acggtgttgt gtatggatga gttcaaatca atgaagtctg tttctggttc
     1861 catgagtttt atttgtgttg atgggacaac ccatcagcta tttacgatcc ttgaagatcg
     1921 cagactttac aaactaactc aatacttcct gc
//
LOCUS       DARBDV010000186         1833 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000186 DARBDV010000000
VERSION     DARBDV010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.196"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..969
                     /locus_tag="SCM56_002903"
     CDS             <1..969
                     /locus_tag="SCM56_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEG1111618.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            1159..1232
                     /locus_tag="SCM56_002904"
     tRNA            1159..1232
                     /locus_tag="SCM56_002904"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1193..1195,aa:Ile,seq:cat)
BASE COUNT          605 a          317 c          346 g          565 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca
     1081 ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatggc
     1141 tttatgtggc aacaaccagt acccttagct cagttggtca gagcagacgg ctcataaccg
     1201 tccggtcgta ggttcgagtc ctacagggta cattaacgta gccatttgaa tcgttctgtg
     1261 ttagaatttt tttgaagagt attatacaag ctaaagcttt tcttcattgc cactcaaatg
     1321 agtggctttt ttatgtatcc ttttatggat taatgaaagg atgtttcaca tagttatact
     1381 tctgtatatt tgaaaagttt tactttgatt tttaaataga aagacatttg ggttaaattg
     1441 tgagataata ataaagaaga gtttaaagcg caccccaaac cacttcccca taagtgtgtt
     1501 acgctttaaa ctcttttata tttgaagcca ttaaaaagca taccatattt ttgaaaaaaa
     1561 gtgagaaaaa aggcttacaa ttggagtggt agttaattag tgacttattt ttgattttat
     1621 agcactgata ctataaaata tagatatcat catattacac aatcttaata ctaacttaaa
     1681 aaatatctcc tttcacaagt atggtgataa aattcgttcc gggctacctt tttaggtagc
     1741 ctactttaat ctttatacct ttctggatca acgaaagtat actttatata gtcataacgc
     1801 cgatgatcgc tacgtgcgtc cggcacgtca gtc
//
LOCUS       DARBDV010000187         1799 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000187 DARBDV010000000
VERSION     DARBDV010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.197"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            29..1381
                     /gene="brnQ"
                     /locus_tag="SCM56_002905"
     CDS             29..1381
                     /gene="brnQ"
                     /locus_tag="SCM56_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEG1111619.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          506 a          309 c          353 g          631 t
ORIGIN      
        1 ttttaaaaaa accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct
       61 ttttataggc tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt
      121 tcatttgggt caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac
      181 cggtatcggt cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt
      241 atatgaatta gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta
      301 cttagttatc ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg
      361 tttagcacca tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt
      421 attttttgtg ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg
      481 aaagttcctg aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt
      541 gaatccacta ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt
      601 tttcacaggg tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg
      661 gatcatcatt gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa
      721 agacacgatc aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct
      781 cgcttattta ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac
      841 gctggctcag attgctgatc attatttagg tacttatggc agtatcttat tagcatttat
      901 cgttatatta gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt
      961 cacagatttg tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc
     1021 ttgtttagcg gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat
     1081 gttcatctat ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa
     1141 gcagcgtacg gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga
     1201 tgggttgaac gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata
     1261 tgttgcggaa agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt
     1321 tatcggtttt gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg
     1381 ataaaagagg ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat
     1441 ggcttctcac atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg
     1501 tttattgaga ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa
     1561 atggcttctc acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac
     1621 cgtttattga gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct
     1681 ccatatactt tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt
     1741 tcttttataa tatagatttt agacatatat atgatataaa aatagttttc gcaaaaaaa
//
LOCUS       DARBDV010000188         1680 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000188 DARBDV010000000
VERSION     DARBDV010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.198"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..221
                     /locus_tag="SCM56_002906"
     CDS             <1..221
                     /locus_tag="SCM56_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEG1111620.1"
                     /translation="NKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIITGF
                     GAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            218..1237
                     /locus_tag="SCM56_002907"
     CDS             218..1237
                     /locus_tag="SCM56_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HEG1111621.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            1426..1499
                     /locus_tag="SCM56_002908"
     tRNA            1426..1499
                     /locus_tag="SCM56_002908"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1460..1462,aa:Ile,seq:cat)
BASE COUNT          539 a          304 c          342 g          495 t
ORIGIN      
        1 ataataaaac gttcgaagta ctaaaatggt tcgcactggt aattattccc gcactagcta
       61 ctttcgtggg gttagttggt aaagcgctca attggcagta cacagatatc tgtgttgtca
      121 tcattactgg ttttggcgcg tttttaggga gtgtgttggg tgtatcaaat cgaacctaca
      181 aaatgttctc ggctgaaagc gaagaaggag gaaacaaatg aaaaagaaaa ttactattac
      241 tgcgatgagc ctgttaacgg ctcttttttt attgccaatt aacggatttg cctatactat
      301 caacaatgaa tttaatttgg gcgcaaatga aggtagctca caagtagcaa ataatcagta
      361 tattttactg catgaaacgg ctaatgaaac agcaacagga cgcaatgaag cgcagtatat
      421 gcaacgttca tggactagcg cttatactgc ttatattgtg ggagacggcg gaattgttta
      481 ccaagtcgga caacctggtt atgtacagta cggtgctggt tcatatgcta atgccaatag
      541 tcctgtgcag attgagttac aacacacaca tgataaagca acttttgaaa aaaactataa
      601 agcatacgtt gaattggcta gagattcagc aatgaaatat ggtattccat taacattaga
      661 cacgccttat aaccaaccag gaatcaaatc acatttatgg gtaactcaaa acatttgggg
      721 cgatcacact gatccgtatg gatacttatc agaaatgggt gtaagtaagg aaaaactggc
      781 ttacgattta gctcatggat ttacagacga aaacccaacg acttctgaga acaagcctgt
      841 cattgatcca acccgagcag gtgcagcaaa tccaacttta tcagatggaa cgaaccattc
      901 tcacattgat cagtttggag aaatcgaaaa tgcgaactta cacgtcgctg gatggcacat
      961 cgctaactat aagtacgagt atattttcat tatggactac aatactggga aagaattagc
     1021 tcgagtaaga gctgatggaa tttatagacc ggatgtaaat caagcttata atacttctgg
     1081 aaatgttggc tatcatgtat cttttaacat gcgtaacttc cctaataaga aagtctatgt
     1141 catgatgcgg gcaacgaatg atccagaggg aaacactaaa ggcggtgcgc aagatttcca
     1201 tgacaaacgt tggtatttaa atattcctaa acgataaaaa tagctcctcg ttgaggagca
     1261 ttacataact atattgacaa ctataaaaat cattcgataa aatagtgatg ttatcgcata
     1321 tcttcactat cacccataat agtcacactc caagctatgc gataacaggt ttgttgccac
     1381 acattctact ggttgattgt ttatggcttt atgtggcaac aaccagtacc cttagctcag
     1441 ttggtcagag cagacggctc ataaccgtcc ggtcgtaggt tcgagtccta cagggtacat
     1501 taacgtagcc atttgaatcg ttctgtgtta gaattttttg aagagtatta tacaagctaa
     1561 agcttttctt cattgccact caaatgagtg gcttttttat gtatcctttt atggattaat
     1621 gaaaggatgt ttcacatagt tatatttctg tatatttgaa aagttttact ttgattttta
//
LOCUS       DARBDV010000189         1677 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000189 DARBDV010000000
VERSION     DARBDV010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.199"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            166..1038
                     /locus_tag="SCM56_002909"
     CDS             166..1038
                     /locus_tag="SCM56_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG1111622.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            1334..>1677
                     /locus_tag="SCM56_002910"
     CDS             1334..>1677
                     /locus_tag="SCM56_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG1111623.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALECFEQYKENKRRCGRTEIIFPAEEKEYIAKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKTG"
BASE COUNT          536 a          311 c          368 g          462 t
ORIGIN      
        1 tatcaattgt atgaaaaatt attagaattt taaaaaatga taaaaattca aaaagaaaaa
       61 atagtagtta tttacttgtc atgttactaa ataaaaaaat aatttaaata tgatatatag
      121 tcatttattt agtgatttca ttctaaaata aagggagaaa tgtctatgct atacggtgca
      181 attgaagcag gcggcacaaa atttgtgtgt gccattggcg atgaagaaat gacgatcaag
      241 gaaagagtga gctttccgac aacgacacct gaggaaacaa tgcctttagt cattgacttc
      301 ttcaaacaat accaagcaga tttagcaggg attgggattg gctcatttgg gcctatcgat
      361 atccatcgcg attccgccac ttatgggtac attacctcga ccccgaaatt ggcatggcaa
      421 aattttgatt tcatcggtac catgaaaaaa gaatttccga tcccgatttc ttggacaaca
      481 gatgtcaatg cggctgccta tggtgagtat gtcttcggca gtggcaaagg attgagcagt
      541 gtcgtttact atacgatcgg tacgggtgtt ggcggcggtg ccttacaaga agggcgcttt
      601 atcgaagggt ttagtcatcc agaaatgggg catatgctcg tcgtccctca tccaaaagat
      661 gactttgctg gcagctgtcc atttcatggc aactgtttag aagggatggc cgctggaccc
      721 gccatcgaaa aacgcttagg gaaaaaagga caagaggttg cagaagatga tccttattgg
      781 gaaatcgaag cctcctatat tgctcaatgt gcctacaata caaccttgat gttttctcca
      841 gatgtgatca tttttggcgg tggtgtgatg aaacaacgcc atatgctgca aaacgtccac
      901 gacgcatttg caaaattggt caatgggtat gtcgaaacac ctgagttaag caaatatatc
      961 gtgaccccag cattagaaga caacgctgga acgctcggtt gtttggcatt ggctcgagaa
     1021 gcggtcttac attcataaac gaaaaaaggc tcttcgacat ttagtgttgg tagagaaaga
     1081 tacaactgaa aatttagtca taaccagtta ggcagagtgc ttaactggtt tttgttttac
     1141 ttggaagatc aagccttgaa tgagataaat tcttgctcga aagttatgga aatttcgata
     1201 gccgtaggcc acttgtattg tgcaatagtt ttttacacgc cgtttgtata attaagcgag
     1261 acaaataaaa aagccattta tgttacactc aaatcgtaat acccgctaaa gcatacaagg
     1321 agagtgaaca taaatgactt atacccatct tacaccaaac gagcttgtaa tgatagaagc
     1381 atattttcat caagaaactc cggttgctat cgttgcgaag cagcttaaac gtggacgcca
     1441 aacaatttac aatgtctata actttctcaa atgtggtgga acagcacttg aatgctttga
     1501 acaatacaaa gaaaataagc gacgttgtgg gagaaccgaa atcatttttc ctgctgagga
     1561 aaaagaatac attgcaaaaa gatcaactga aggttggacc ccagacgtca ttattggccg
     1621 tgcagagcga accttctctt gttcagtaag taccctctat cgccggttta agacggg
//
LOCUS       DARBDV010000190         1664 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000190 DARBDV010000000
VERSION     DARBDV010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.200"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            131..505
                     /locus_tag="SCM56_002911"
     CDS             131..505
                     /locus_tag="SCM56_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEG1111624.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="SCM56_002912"
     CDS             complement(619..1248)
                     /locus_tag="SCM56_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEG1111625.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="SCM56_002913"
     tRNA            complement(1382..1454)
                     /locus_tag="SCM56_002913"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DARBDV010000191         1643 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000191 DARBDV010000000
VERSION     DARBDV010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.201"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            133..1599
                     /locus_tag="SCM56_002914"
     CDS             133..1599
                     /locus_tag="SCM56_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEG1111626.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          462 a          296 c          337 g          548 t
ORIGIN      
        1 atgatattgc cattaaaaat tacttgcttt tatagaaaat tagtttatat ttcttaaata
       61 agttttttag ttaaattttt cggtaaagat gtataatatt ttctgaaaat ttaaagaatt
      121 ggggtataga ttatggaaaa acagcaagac aagggcttct tcgggcagcc gaagggattg
      181 tcaactttgt tcttcactga gatgtgggaa cgatttagtt attacggtat gcgtgcatta
      241 cttatctatt atatgtacga tacagttgcc aacgggggac ttggattacc acgagcaaca
      301 gcattagcta tcatgtcgat ctacggatct ttgatctaca tgtctagtat cgtcggcgga
      361 tgggtatctg accgagtgtt aggagcaaaa cgcactgtat tcttcggtgg agttttcatt
      421 atgcttggcc acatcgtctt ggctttacca tttggtgtaa gtacattgtt tgtttcgatg
      481 gcattgatta ttatcggaac cgcctttttg aaaccaaatg tttctggcat ggtcggacat
      541 ctttattcaa agactgactt gcgaagagat gcaggatttt cgatttttta catgggtgtc
      601 aacttagggg cattattagc accactaatc gttggtacaa tcggtcaaga atataactat
      661 catttagggt tctctttagc tgctattggg atgttctttg gattattaca atacatcatc
      721 caaggacgca agacattaga cggagtgggt acaacaccgc cgaatccttt gacaactgaa
      781 gaacgtaaag gattcatccg taacttgatc atcgcacttg ttgttatcgc tgcagtattc
      841 ggcggggcac aactaactgg tcatctgaca attgactttt ttgtcaatac aatcagtgta
      901 ttggggatta tcttaccgtt gtattacttt atcaaaatgc tgacttcaaa agatgtaact
      961 gctgaagaaa aaccaagagt atgggcttat atcccattat tccttgcagc aatcattttc
     1021 tggtcattac aagaacaagg ttcatctatc ttagctttat ttgctagtga acgtacacaa
     1081 tctacgctat ttggtgtaac gatccaagca agctggtatc aatcattgaa tccaattttc
     1141 atctttattc tgacaccagt attcgtaacg atgtggacga aacttggtga acgtcaacca
     1201 tctacagtag tgaaattctc tcttggactt ttatttgcag gattatcgta cttgctattg
     1261 atgttgccgg gtatgcttta tggtacaagt ggaaaagtaa gtccattgtg gttgttggga
     1321 agtttcttca tcgtggttat tgctgaattg tgtctttcac cagttggttt gtctgtgaca
     1381 acaaaattag caccacgagc atttgaatca caaacaatcg ctatttggtt cttagctgat
     1441 gcttcttcac aagcaatcaa tgcacaaatt gcacgcttct acacaccggg aacagaatct
     1501 gcttacttcg gtattatcgg tgcagtagca atcgtcggtg gtatcttgct atttactgtg
     1561 aaagaaccaa tcaaaaaact aatgggctct attcattaat caatgaacat aaaaacagga
     1621 attgcttatt tgcagcagtt cct
//
LOCUS       DARBDV010000192         1547 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000192 DARBDV010000000
VERSION     DARBDV010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.202"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            86..1144
                     /locus_tag="SCM56_002915"
     CDS             86..1144
                     /locus_tag="SCM56_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEG1111627.1"
                     /translation="MVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPGLLARQGF
                     IEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQIFYITLSS
                     SRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAK
                     DFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVI
                     RPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAF
                     ICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFNKRDVISI
                     VFWYYLGSISYLYLKKEN"
     gene            1146..>1547
                     /locus_tag="SCM56_002916"
     CDS             1146..>1547
                     /locus_tag="SCM56_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111628.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFS"
BASE COUNT          511 a          183 c          254 g          599 t
ORIGIN      
        1 ttataatttt ggatatttgc tgattacttt gatagtaatt tttcctgcag acttttcaca
       61 aagtgaagag gaaaaaaaga aaaaaatggt gtattttaat aatgttttca ttatcataat
      121 ttttttagct gcgctaatat ctatctatca atttattttt ttgatttctt atcatgtccc
      181 aacaggaacg ccaggattac ttgcaagaca aggttttatt gaacatcggt tatttggtgt
      241 ctatacaagt ccaaatgttg gagctatgtt tggatatact tctattattt taagtttaat
      301 tagtgtatta atttcaaaaa taacaaaaac gattcgtgtt ttttatatga tgaattttat
      361 cgtacaaata ttttatatta cactttcttc gtctagaggt gcccaggtag taatttgttc
      421 ttttttagta ttatttcttt tgttgtttgc caattcaaaa tttagaaaga gcattagaga
      481 agtactacct gtaaaaatca aatggatggt aagcatatta ttagttctat tggtatattt
      541 ttcttttggt actaattatg ctaaggattt tttagcaaca attcctgtat cgattgatag
      601 aaaagtagaa gttatagagt caaatgaatc agataaaact ccacttccag aaaaagaagt
      661 aatattacag cattctagtg ataactccga aatttcagct ggtcgtttta cgatttggaa
      721 agctgcgctt aaagttataa gaccaacttt gttatttggt tatggagaga ccgatttcta
      781 tgggaatagc gatgcaaaat ttttgaaaaa agtagatctt gatactacag atgtgaatga
      841 actaaaaaga gcacatggaa acatgcataa tggatttctt caagtacttg tatcaagtgg
      901 tataattgct tttatatgta tctatgcttt ctatgtgtta aatatcttta gtttactcaa
      961 aacatttttt agaagtaaag tgaattatgc gtttcttggt attgttttaa tttttatact
     1021 atctattttt atagatgaaa tggtggaggc tcatgtactt ttcaataagc gcgatgtcat
     1081 ttcaattgtt ttctggtatt atttgggaag catttcttac ttgtatttaa aaaaggagaa
     1141 ttaatatgaa gacagctttc attgcttgga ctcctttaca tttgattaat atattaaatg
     1201 tttatcaaaa tttttaccga gaatcaaaag gagatttatt tatttattct gaatttgaaa
     1261 acgctagaag atatagtgag cgtataaaag aattagggct atttaatgat gttgtttttg
     1321 tgaatcaccg gaaaatcggt tcttccttta aacgtaaaat taacttgtta actaaccata
     1381 atcaattttt ctcggatgat ttatccgttt atgatgaaat ttttatacag ggggaaaatt
     1441 atttcacaaa aatacttttc tccaatgttt acaaaaaaaa tccagatgta aaactaaatt
     1501 atatagaaga tggtttgggt acttatttga agaagagtat attttca
//
LOCUS       DARBDV010000193         1500 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000193 DARBDV010000000
VERSION     DARBDV010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.203"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            316..570
                     /locus_tag="SCM56_002917"
     CDS             316..570
                     /locus_tag="SCM56_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEG1111629.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="SCM56_002918"
     CDS             571..888
                     /locus_tag="SCM56_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEG1111630.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          531 a          228 c          290 g          451 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 gacaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaacctt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacttc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
//
LOCUS       DARBDV010000194         1486 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000194 DARBDV010000000
VERSION     DARBDV010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.204"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            288..695
                     /locus_tag="SCM56_002919"
     CDS             288..695
                     /locus_tag="SCM56_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111631.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            709..1110
                     /locus_tag="SCM56_002920"
     CDS             709..1110
                     /locus_tag="SCM56_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111632.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1112..1483
                     /locus_tag="SCM56_002921"
     CDS             1112..1483
                     /locus_tag="SCM56_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111633.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          570 a          183 c          259 g          474 t
ORIGIN      
        1 ttgaggggaa gtacatatag ttgttgatat agttgctaat attgttagct aatttgttag
       61 taaaactgta tacgaattta gatagtaaaa aaacagtttg ttccttatat agaggcactt
      121 ttactactga ttaaatgtca gatatgctta caaatatact tataaatatg cttacaaaat
      181 tagctaatat ttccgttgac aaagctttgt tactattgtt aaactaaatt gactagcaaa
      241 agtgaaacat aaatttatta aataaatgta aaaattagga gaaatcaatg tcttatagag
      301 tacaattaat aatttcagaa gatgtagaaa gtcagcaatt tggcactaat gttattaata
      361 aagtaattaa tccagctctt tctattaatg cgcctttaat tcctactgct ttatcatttt
      421 ctgtaacagc tgtggtgtct gagatagagg atacagaaaa gataaaaata gtagaaattg
      481 aagttttaaa caagaatgaa aaacaaattt tttctacggg tgaagtatcg gtaagcttgc
      541 ctcctcaagt taacgatata aactttaata taaacgccag aaatgtcttg gttgaagaag
      601 caggagagca ttatgctgtt gttaaattta atggaactga gattggaagg caaatctttg
      661 atatcaaggt caacaaacca gtggaaaaaa attaaggaga cgatacggat gcttgatata
      721 gtaccatcaa atacacatcg gagtggtagc ttaataaatt ttgctgcgat ttttgcttgc
      781 gcatcggtaa ttctagcatc tccagttgtt gtggatgcaa gttctactcc gaaaactaaa
      841 aatgataatc aacttgtaat tactacaaaa aatgagatta acacaatttc aagtaatgat
      901 ggcaaaattt ttgatgtgat aaatacggtt ttaaaaagaa aagctgaaat caatataaaa
      961 tatgatgaag atttaaactt gtatttcttt gttatcaaaa caacatctga attgttttcc
     1021 tcagattacg atgttttgga tactctcgat aatgtattat ctgactataa atatatgggg
     1081 aaatctgtag tagcaacttt gggggaataa aatgtttgac tttaatgaat atttatctat
     1141 ttccaaacaa attgaatcag atgataaata taattctaaa gaatcttata gaagaactgc
     1201 tgtttcaaga gcatactaca gtgcttttaa aaaatctgat gagtatttga aagaaaatta
     1261 tgatattatt tataatggaa gtagcggaaa aggaagccac caaaccgttt ggaatttgtt
     1321 ttcaacagtt aaggaattga ataccttagg aatacataat agtgggtata gaatgttaga
     1381 gaaaagaaaa tgtgctgact atattcctaa tgaaaccatt acgaaaacag atatggcgct
     1441 gatgaatcga gaagcagaaa aaataataaa caaactcacc taatga
//
LOCUS       DARBDV010000195         1477 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000195 DARBDV010000000
VERSION     DARBDV010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.205"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(62..280)
                     /locus_tag="SCM56_002922"
                     /pseudo
     CDS             complement(62..280)
                     /locus_tag="SCM56_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(546..740)
                     /locus_tag="SCM56_002923"
     CDS             complement(546..740)
                     /locus_tag="SCM56_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111634.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(840..926)
                     /locus_tag="SCM56_002924"
                     /pseudo
     CDS             complement(840..926)
                     /locus_tag="SCM56_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          517 a          231 c          195 g          534 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattgta ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt attacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt tataattagc aagtatatat
     1321 atttaaaaat ttaagattaa tttttttatg acttgtacaa atttatctga tattctctta
     1381 ttaagaacta cctaatttgg gtagttctta ttttttacat tttaagtagt atttaattgc
     1441 aatttggtag tattttttta ttatcttatt cttagaa
//
LOCUS       DARBDV010000196         1454 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000196 DARBDV010000000
VERSION     DARBDV010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.206"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..625)
                     /locus_tag="SCM56_002925"
     CDS             complement(<1..625)
                     /locus_tag="SCM56_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HEG1111635.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFD"
     gene            complement(784..1050)
                     /locus_tag="SCM56_002926"
     CDS             complement(784..1050)
                     /locus_tag="SCM56_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1111636.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1040..1396)
                     /locus_tag="SCM56_002927"
     CDS             complement(1040..1396)
                     /locus_tag="SCM56_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEG1111637.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          406 a          279 c          181 g          588 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
      361 ctggagaacc caacttatgg ccaaaaatcg ctaataatat atcactattt tctacaattt
      421 gtttattaat aacactttgc ccagagctat tatttatata agtaggaatc gcattcgttt
      481 cccatcgaat tggcatcaaa attacttttg tattatctgt attttctata ttccatctat
      541 atatggtttc ttctacttca ttcctctttg ctgctatatc acctggtgat gctataaata
      601 cttttattag atttatggtt ttcatttatt gtacctttct ctttatgtct ctttaaagta
      661 atttttgtat aaaatttatt gacttttaca ctataggaca acttttatct atttagtaca
      721 aaaaggccca ctaaactaat tgattagtta ggagcctatt acaggtatat tttcaactcg
      781 cttttaagca aattctacta ccagcttttt gcctaatgcc ctagcgatat catcaagttt
      841 atcgaaagat acgttatccc ctctctcaat tcgagcaata gtagattgag ttgtatgtgc
      901 tttttgtgca agctccgctt gagttaggcc tgcttcctcc cgtgcatgat aaagagcaac
      961 cgcagtttcg agttttcttc cttcacggtc atatgcttct gcaaaatctt tgtctttttg
     1021 cttttcttca atcaattttt taatcatcat ttgtgaacca ctcctctcta agttcttttg
     1081 catgtttaag ttcacttatt ggtgttttct ccgttttttt agtaaatcca tgggtaatga
     1141 cataccttcc attgaccaca tggaaataga tagctctttg gatgtttgat cctaccttac
     1201 ttcttaattc gtacaaatta gtgtctaatt ttttcaccca tttcaaccgt tgagctacta
     1261 gcatcccatt cttttctgtt tcttctattg tacgtaaaag cttcgcacta tctttttttg
     1321 gtaatgtttc caaccattct aaaaattcat catgaccatt aggtctttta aatgtttcaa
     1381 aagatatttt ttccataccc aattatatca tatattgatt ttatattata gtatataaca
     1441 taaaaattcg aatt
//
LOCUS       DARBDV010000197         1440 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000197 DARBDV010000000
VERSION     DARBDV010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.207"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            105..1406
                     /locus_tag="SCM56_002928"
     CDS             105..1406
                     /locus_tag="SCM56_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEG1111638.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          474 a          288 c          264 g          414 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttcttat
//
LOCUS       DARBDV010000198         1417 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000198 DARBDV010000000
VERSION     DARBDV010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.210"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(150..1337)
                     /locus_tag="SCM56_002929"
     CDS             complement(150..1337)
                     /locus_tag="SCM56_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HEG1111639.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DARBDV010000199         1357 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000199 DARBDV010000000
VERSION     DARBDV010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.211"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..318
                     /locus_tag="SCM56_002930"
     CDS             <1..318
                     /locus_tag="SCM56_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEG1111640.1"
                     /translation="RRNPYFNMVKRIENEVTIVNKSRYTSRQQTPEVFEMNASMYSFK
                     PEFLKKSNYIFDGQCGIIKMRDYLILDIDSEEDFKWLEFLFPKFLEDSSELQDIYNYL
                     YRR"
     gene            323..1270
                     /locus_tag="SCM56_002931"
     CDS             323..1270
                     /locus_tag="SCM56_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEG1111641.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          455 a          193 c          277 g          432 t
ORIGIN      
        1 agacgtaatc cctattttaa tatggtaaaa agaattgaaa atgaagttac tatagttaat
       61 aaatcaagat acacatctag gcaacagaca cctgaagttt ttgaaatgaa tgcctcaatg
      121 tattctttta aacctgaatt tctaaaaaaa agtaattata tttttgatgg gcaatgtggg
      181 ataataaaaa tgagagatta tctaatttta gatattgata gtgaagaaga ttttaaatgg
      241 ttagaatttt tgtttcctaa atttttagaa gattcttcgg agcttcaaga tatctataat
      301 tatttatatc ggaggtaaaa ggatgagctg ttatcttatc accggtgggg ctggcttcat
      361 tggttctacc ttagccaatc atttaggaga agaacatgtg gtaatcgtcg tcgatgactt
      421 atcaatggga aaagtagaaa atcttgatat gacgaaaaat atcactttta ttgagggaga
      481 tgtcgctgag aaatccttga tggaagagat catgaaagct aataaatttg attatatctt
      541 tcatttagca gcagttgcta gtgtggcaga ttcagttgcc cgaccagtag aaacacatcg
      601 tgtgaatttt gaaagtgtat taatgatttt agaattgatc cgcaagtatc aacctgattt
      661 aaaacggatc gtgtttagtt cttcagcggc tgtttatggg gatgaaccga ctttaccaaa
      721 aaaagaagag tccgtgattc gcccattgac gccttatgcg gttgataaat tcgctgcaga
      781 acaatatgtg ctagattatt gtcatttata tgatgtaccc ggtagcgcag ttcgtttttt
      841 caatgtctat ggtccaaatc aaaatccgaa ttctccatac agtggtgtga tttctattct
      901 cgtagatcgc tataagaaac aaatcgtagg agaagcaact tcatttacat tatttggtga
      961 tggaagccag tctagagact ttgtttatat agatgatgta atccaagcat tacttttagt
     1021 tgcgaatgag gaagctgcac tggggaaaca attcaatgtt ggaacgggta aagcaaccac
     1081 acttttggaa ttgattcatg caatcaatga aattctcggt gtagaattga cacttgaata
     1141 caaagaagag cgttcgggtg atatccatga ctctctagca gatattacta agatccagtc
     1201 aattggctat caaccgaaat atggtatttt aagtggaatg actaattatt tgaaaactga
     1261 aatcaattaa aacatactaa gataagatta gagggtaaat tcaggctctt tgtcaataag
     1321 gactgatgag ttttgtaaaa tcaaatctgg gatagaa
//
LOCUS       DARBDV010000200         1355 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000200 DARBDV010000000
VERSION     DARBDV010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.212"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1355
                     /locus_tag="SCM56_002932"
     rRNA            <1..1355
                     /locus_tag="SCM56_002932"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DARBDV010000201         1294 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000201 DARBDV010000000
VERSION     DARBDV010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.213"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1244
                     /locus_tag="SCM56_002933"
     CDS             <1..1244
                     /locus_tag="SCM56_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEG1111642.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DARBDV010000202         1287 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000202 DARBDV010000000
VERSION     DARBDV010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.214"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..848
                     /locus_tag="SCM56_002934"
     CDS             <1..848
                     /locus_tag="SCM56_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEG1111643.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
BASE COUNT          418 a          235 c          252 g          382 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttttc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgcgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aacgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggaaga accatttaca caaaattatt gacgctccca
      901 agctaaaact cctattaatc gatctactaa aaataaataa aaaaattctt ttcaattttt
      961 atttatggta tgacataaat aaagtaaagt tattcttcta ggttaatctt attaatactc
     1021 tttctcacta gtgttgcatg caacactagt gagaaagagt attaaatata ctgcaaggca
     1081 agacatttgg taattgaaga ataacaagca aaaaaatagc agtcaggtct cttgacaaag
     1141 tatagttgct attctttttt taggccctac tttaatgaaa tttttgtttg gagggaacga
     1201 cttcattcct ttgattattt cagttgaatt taatagagaa gcctggcgtc tattaagaaa
     1261 tacttttctt gatgagcgtc aattttt
//
LOCUS       DARBDV010000203         1275 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000203 DARBDV010000000
VERSION     DARBDV010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.215"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(31..1203)
                     /locus_tag="SCM56_002935"
     CDS             complement(31..1203)
                     /locus_tag="SCM56_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HEG1111644.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRL
                     KSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ"
BASE COUNT          345 a          265 c          245 g          420 t
ORIGIN      
        1 taaaatacta tacaatgttt taccggtctc tcattgatca aacttaatat attttcttgt
       61 agaactgagc cattcgtcat gaaggtccat aaggacagct ccaattaatc gattggcaga
      121 cgttcggttg ggaaaaatcc gaataatctt ttctcttctg cggacttcct ggttcagtcg
      181 ttcaagaagg ttggtgcttt ttagccgatt atgactattt ccgataaccg tgtattgaaa
      241 ggcatcttcg aagccattat ccaatatttc gcaggctttt gtatatttag gctggtcgat
      301 atattcaccg actaaggcat tcttagctgt tcgagcgagt tcaatatccg taaacttaaa
      361 gatcgctttt actgcttctc taaacggttt tgaattcttt tttggaatgg aactgaagat
      421 gtttcttaaa aaatggacct ggcatctctg ccaacttgcg ttggtaaatg acttacgaat
      481 cgcagacact aggcctttat gggcatcaga aatgatgagt tctgtcccct ttaggccgcg
      541 ttcttttaag tattcaaaga agatggacca tgtgtcatca ctttcttcat tttgaatcat
      601 gaagccaatg atttcacggt cgccaccttc tgttatcccg atcgcaatat ggcagctttt
      661 agaaagcact cgatggtcct cacggacttt tatgtagaga acatcagtca tcagataagg
      721 ataattcgta cctgagagtg aacggttctg ccattcgttg accatcgggt ctaactgctc
      781 agtcaggcta gaaacaaaag atttcgatac agattttcca catagctctt caacaatctt
      841 tgaaacttta cgtgtcgaaa cgcccgaaac atacatctca agcattgaag cgagcagtgc
      901 cttttcattt cgctgataac gctcaaacac cgtcggtgaa aattcaccat cacgtgttct
      961 aggcactttt aattcgagtg tacccacacg agtcgtaaag tctcgctcat aatagccatt
     1021 tctttgactc tgacgacttt ctgaacgttc atagtcatcg gcttgaatat attctgttcg
     1081 ttgattttcc atcaattggt tgaaaacagt ggttaaaata tttttagaaa catcatcttt
     1141 taccgaatgt tcaataatac tttgaacctc ttcgttgttc agtgtaaaat gtacttgggt
     1201 catgtaacgt cctcctgggt atgtttttgt ggttaaaaac attgtaccgt aaaagggctg
     1261 ttacatggcc tttta
//
LOCUS       DARBDV010000204         1263 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000204 DARBDV010000000
VERSION     DARBDV010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.217"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            22..1101
                     /locus_tag="SCM56_002936"
     CDS             22..1101
                     /locus_tag="SCM56_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG1111645.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT          335 a          342 c          356 g          230 t
ORIGIN      
        1 aatagacaag gatgaattca catgacgcaa aaccatgata acacagtttc acgcaaggga
       61 aaacacctaa cctactcaga acgttgccaa atcgccatcc tgaaaaagga aaactattcc
      121 aaccgtcagg ttgcggatgt cctgggctgc gtgccgcaga ccataaacaa cgaaatcaaa
      181 cgcgggaccg ttactcagct taaccgccag aatcagaacg ggaaggtcta cgactattat
      241 catgacgtct acgacgccga tgccgggcag gcggcctacg agaagaaccg cttgaactgc
      301 ggcagacggc ccttatgggc cgacacggac gccttcattg aatgggcgga cgacatgatg
      361 ctgttggaaa aatggtcccc ggacgtggtg gtgggctttg ccagaaaaca cgaattgttc
      421 gacccggcca ttatccctgg gaccaccacc ctctatcagt ggatcgaccg tggcatcatg
      481 aaaacgaaga atatggacct gctagaaaaa ctgtcccgca aaccgaaatc gtcccgtcat
      541 ggcaaccgcc cgaataaacg ggtgctaggg aaatccattg aagagcgccc ggaggcggtt
      601 ggtacccgga aaaccttcgg ccattgggaa gtcgacacgg tcgtaggaaa caagaccaag
      661 tcggatgccg tgctgctgac gttggtggaa cggcagacac gcttcgaggt cattctgaag
      721 ctggccggga aggatgccga atcggtcaac caagcggtcc gacagctgag agaacgggct
      781 ggtgcgtact tcggccagct gttcaagacg gttacctcgg acaacggctc ggagttcgcg
      841 ggactgcacg atgccttgca ggatgtggca aacatctact tcagccaccc ctatgcttcc
      901 tgggagcgcg ggacgagcga aaaccaacac aagctgattc gccgtttcat ccccaagggc
      961 agcccaatcg gcaacgtgag cgaaaaccaa atcttgcgga ttcagcgttg gatgaacgat
     1021 tacccgcgaa agattttggg ctatgcaacg ccgcatgaca cgttcgtcca ggcatttagg
     1081 caggaacggt tgacggcttg acacgtgggc ccttgacaac gagcctccct cggcatggct
     1141 tgtgtctgga aagctggccg gggccagcca gtcgggagac taccatgcct cagctcattg
     1201 tcaagggccc actcgattag aaaaattaga cggttggtgg taacgagcaa ccgggtcacc
     1261 atg
//
LOCUS       DARBDV010000205         1246 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000205 DARBDV010000000
VERSION     DARBDV010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.218"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            200..742
                     /locus_tag="SCM56_002937"
     CDS             200..742
                     /locus_tag="SCM56_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111646.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1246
                     /locus_tag="SCM56_002938"
     CDS             898..>1246
                     /locus_tag="SCM56_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEG1111647.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DARBDV010000206         1212 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000206 DARBDV010000000
VERSION     DARBDV010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.219"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>1212
                     /locus_tag="SCM56_002939"
     CDS             <1..>1212
                     /locus_tag="SCM56_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HEG1111648.1"
                     /translation="RITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRACRSCGSTVV
                     DGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSYFVQPRHS
                     IANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSKKILPRVLMV
                     DEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEEVEFLVTDMN
                     AAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKSQAEKLKKNW
                     RFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAYPFFHELVEA
                     FRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNGKIEAKNTHI
                     KTMKRVSYGFK"
BASE COUNT          401 a          241 c          237 g          333 t
ORIGIN      
        1 tgagaataac agagaaagat ttgatgatta cagaggtctc ttacgagacc cttcagaaga
       61 aaaagacgtt ggtcgtcgat gctgttctct cgcctactcc tcgtgcttgt agaagttgtg
      121 gttctactgt ggtagatgga aacgggaaag caattatagt gaaaaatgga aaaaaggaaa
      181 cgattgtccg ttttgaacaa tacaatcata tgcctttggt tatgcgccta aaaaagcaac
      241 gctatacctg taaaaactgt cgcacccatt ggacggctca aagttatttt gtccaaccca
      301 gacattcaat cgcaaatcat gttagatata aaattgcttc tttactgact gaaaaagtat
      361 ctttatcttt tattgcgaaa agctgtcagg tatctttgac caccgttatt cgtacattga
      421 aagagtttaa aagctattta ccaaagcaat ctaagaagat cctccccaga gtattgatgg
      481 ttgacgaatt tcgttcgcat gcttccatag aagataagat gagctttatt tgcgcggatg
      541 gcgaaacagg aaaattaata gatgttttgc ctacgcgtaa attacctcga ttaacaagct
      601 atttcttagg ttgtaccaat ccagaagaag tagaattctt ggtgacagac atgaacgccg
      661 cctacttcca gctcaccaaa cgtgttctgc caaatgcgaa agtcgtgatt gatcggtttc
      721 atattgtcaa acacatgaat caagcgttca atgagttgcg tatccgtgaa atgaatgaac
      781 ttcgtaaagc cggacagaaa agccaggcag aaaaactgaa aaagaactgg cgctttttgc
      841 taaaaaatcg tgcaaacatc aaccattatg aatacaaaac atggaaaagt ttccgagcac
      901 caaaataccc atttcttact gaagcaatga tgattgatcg attgcttgag ttttctccgc
      961 ccctaaagga ggcgtatccc ttttttcatg aattagttga agcctttcga gacaaagacc
     1021 ctgacttatt tttctcctta ttggcagaac ttcccgaaac gttagatgac agctttcggg
     1081 aaaagcttca aaaccttctg acctatgaag aaggcatcac caacgcaatg atctatcctt
     1141 attccaatgg aaaaatagaa gcgaagaata cccacataaa gacaatgaaa cgagtatcct
     1201 acggatttaa at
//
LOCUS       DARBDV010000207         1207 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000207 DARBDV010000000
VERSION     DARBDV010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.220"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>1207
                     /locus_tag="SCM56_002940"
     CDS             <1..>1207
                     /locus_tag="SCM56_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HEG1111649.1"
                     /translation="QKKKTLIVDAVFSPAPHTCRNCGSTVVDGNGKVIVVKNGKKETI
                     VRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSYFVQPRHSIANHVRYKIASLLTEKV
                     SLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRSHASIEDKMSFIC
                     ADGETGQLIDVLPTRKLPRLTSYFLGCANPEEVKFLVTDMNAAYFQLTKRILPNAKVV
                     IDRFHIIKHMNQAFNELRIREMNELRKVGQKSQAEKLKKNWRFLLKNRANINHYEYKT
                     WKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAYPYFHELVEAFRDKDPDLFFSLLAELP
                     ETLDDGFREKLQNLLTYEEGITNAMIYPYTNGKIEAKNTHIKTMKRVSYGFKSFENMR
                     IRIFLINQL"
BASE COUNT          400 a          234 c          232 g          341 t
ORIGIN      
        1 ttcagaagaa aaagacgttg atcgtcgatg ctgttttctc acctgctccc catacctgta
       61 gaaattgtgg ttctactgtg gtagatggaa acgggaaagt aattgtagtg aaaaatggga
      121 agaaggaaac gattgtccgc tttgaacaat acaatcacat gcctttggtt atgcgcttaa
      181 aaaagcagcg ctatacctgt aaaaactgtc gaacccattg gacggctcaa agttattttg
      241 ttcaacccag acattcaatc gcgaatcatg tcagatataa aattgcttct ttactgactg
      301 aaaaagtatc tttatctttt attgcgaaaa attgtcaggt atctttgacc accgttattc
      361 gtacgttgaa agagtttaaa agctacttac caaagcaatc taagaggatt ctccccagag
      421 tattgatggt tgatgaattt cgttcgcacg cttccataga agataaaatg agctttattt
      481 gcgcagatgg cgaaacagga caattaattg atgtcttgcc tacgcgtaaa ttgcctcgat
      541 taacaagcta tttcttaggc tgcgctaatc ctgaagaagt aaaattttta gtcacagaca
      601 tgaacgccgc ctacttccag cttaccaaac gtattctgcc aaatgcgaaa gtagtgattg
      661 atcggtttca tattatcaaa cacatgaatc aagcctttaa tgagttgcgt atccgtgaaa
      721 tgaatgaact tcgtaaagta ggacagaaaa gtcaggcaga aaaactgaaa aagaactggc
      781 gctttttgct aaaaaatcgt gcaaacatca accattatga atacaaaaca tggaaaagtt
      841 ttcgagcacc aaaataccca tttcttacgg aagcgatgat gattgatcga ttgcttggct
      901 tttctacgtc cctgaaggag gcgtatccct attttcatga attagttgaa gcctttcgag
      961 acaaagaccc tgacttattt ttctccttat tggcagaact tcccgaaacg ttagatgacg
     1021 gctttcggga aaagctccaa aaccttctga cctatgaaga aggcatcacc aacgcgatga
     1081 tctatcctta caccaatgga aaaatagaag cgaagaatac ccatataaag acaatgaaac
     1141 gagtatccta cggatttaaa tcatttgaga acatgagaat tagaatcttt ttgatcaatc
     1201 aattaat
//
LOCUS       DARBDV010000208         1193 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000208 DARBDV010000000
VERSION     DARBDV010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.221"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1076
                     /locus_tag="SCM56_002941"
     CDS             <1..1076
                     /locus_tag="SCM56_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEG1111650.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DARBDV010000209         1144 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000209 DARBDV010000000
VERSION     DARBDV010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.222"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(19..924)
                     /gene="xerC"
                     /locus_tag="SCM56_002942"
     CDS             complement(19..924)
                     /gene="xerC"
                     /locus_tag="SCM56_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEG1111651.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          338 a          232 c          194 g          380 t
ORIGIN      
        1 ttgttctttt ccttattttt acgctcttgg atggaaagaa cgatagtttt tttgtaaact
       61 ttcttttgtt acatgggcat agatctgggt agtcgataaa ttggcatgtc ctagcaattc
      121 ttggactgtc cgcaaatctg ccccattatt taataaatga gtagcaaatg tatggcgcag
      181 catgtgagga tggatatcac tatccaaact gctttttttg atcatttgat tcaagacata
      241 ttcaattcca gtaggcgtga ttggttttcc atgatgattc acaaaaacga tatcatgctc
      301 ttcgtgatat tttgtcatca atacctggcg gccttccctg aaatattctt gcagagcatc
      361 ttgagcaaaa gaacctagag gagcatatct ctctttgttc cctttaccat ggatcaacat
      421 gacctctgaa tcaaaatcaa tgtctgatac cgacaaactg ctacactcac ttaaacggat
      481 gcctgacccg taaaggactt ccagtaaagc gcggttcctc aaatccaaag gactatctcc
      541 tgctacgctg tcaaaaagtg cttgcatttc attttcataa aaaaaacgtg gcagtctcaa
      601 atttttcttt ttgagatgaa cgtaagaaaa tggattttct tttatcactt cttctttgag
      661 aagatactga taaaacgaac gtaggctggc tatcttgcga ctgatagaat ttctgctata
      721 gctaggacga tgatcagcta atgcactcaa atagacacga accgtaaaat attccgcctt
      781 cagcagatca gcgtctccag attcctccaa aaaatgtttg aaatggagaa tgtcttcttc
      841 atatgcttct ttcgtttttt ctgaatatcc tctttcgaca atcaaataac gcaaaaattt
      901 ttgttcccac gtggcttctg tcattttctc accttccttt tgtcccagag aataatttag
      961 cacagcgttt ataaaaagac aaataaattg tcagaattta accaatattt ctaagtttgt
     1021 tcacaattgg cgcaattcct cacattttgt ttgagaaatt cttaagaaaa acgcaagaaa
     1081 aagggctcta taatttatgg gactgatgaa tcagaattga ttcttcagcc ccattttcat
     1141 ttta
//
LOCUS       DARBDV010000210         1106 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000210 DARBDV010000000
VERSION     DARBDV010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.223"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1087
                     /locus_tag="SCM56_002943"
     CDS             <1..1087
                     /locus_tag="SCM56_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1111652.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          403 a          192 c          214 g          297 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaa
//
LOCUS       DARBDV010000211         1063 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000211 DARBDV010000000
VERSION     DARBDV010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.224"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..153
                     /locus_tag="SCM56_002944"
     CDS             <1..153
                     /locus_tag="SCM56_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEG1111653.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
     gene            159..575
                     /locus_tag="SCM56_002945"
     CDS             159..575
                     /locus_tag="SCM56_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111654.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            681..944
                     /locus_tag="SCM56_002946"
     CDS             681..944
                     /locus_tag="SCM56_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEG1111655.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            944..>1063
                     /locus_tag="SCM56_002947"
     CDS             944..>1063
                     /locus_tag="SCM56_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEG1111656.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPY"
BASE COUNT          407 a          132 c          215 g          309 t
ORIGIN      
        1 aataaatttt atcaaagtct ccgtacagcc tcttccacga ttaagggcat ggagaccatt
       61 cgaggaatat ataaaaagaa ccgaagaaat ggaacgctct tcagcttttc ggtgtctact
      121 gaaatcaagg tattaatggg aataacagcc taagatattt ggagttcaga gagggcgcgt
      181 ttgattttca aacttcgcaa cagaaccgaa aatttatacc ggaagatttt caaaacttac
      241 agttagaaaa catgcgttta gaagatttat ttagattagc agatgatgat aagtatagat
      301 ttgaagtttt gaaaattaaa aagaatgaac tattgaataa gttatatcct gagaatgcga
      361 taaaagcaca ttttggtgat gctaatcata tgtatagttg gttcattcct aaaagattta
      421 gagggaaaat caatgctgga gatcaagtga aagtactcaa tcgaaatagt acagatgtcg
      481 taatgataac agatatctac gtagaagatg aagttataat caaatatatg aagcctgtga
      541 taaaacttgt taataaaaat aaaggaaacg gataacactt tacaatgctg ttttggtact
      601 ccaatttaaa caaaacaatc gaatttacgt acttaattaa gtacgttaga atggagatat
      661 aaattagagg gggagtagat atggcaaaaa cagtcttaaa tcctagctca gcacggaaaa
      721 atttttataa gttgttgaaa gatgtaaatg agaatcatac ggaaattgaa atcattagtg
      781 agcgcagtga aaataatgcg gtgcttattg gtttggatga ttggaaagca atcaaagaaa
      841 cgctattact tgagcaaata ggcactttgg gtgttgttcg taagcgagaa aaagatgata
      901 gtggatttac taatgttgat gaaattgatt gggataactt ataatgaata actattctgt
      961 gatgattaaa aactcggcta aagctgattt aaagaaaatc aaacagtcta atttgaaacc
     1021 tcaatttgag aaggttattc agactttaaa ggaagatccc tat
//
LOCUS       DARBDV010000212         1063 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000212 DARBDV010000000
VERSION     DARBDV010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.225"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            89..1024
                     /locus_tag="SCM56_002948"
     CDS             89..1024
                     /locus_tag="SCM56_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG1111657.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT          371 a          168 c          241 g          283 t
ORIGIN      
        1 gttataaaaa tgaacattgc atagtagaga ttctttgatt atactaattc aaggttatat
       61 gaaaaaagag aaatgagatg gatgatttat gaaactttgg atgaaaatcc ttttaggcgt
      121 cgctttagct ctgattgttg cgttagtgtt tgcagggaat tatttttata attatgctgt
      181 tgtgccttct gagaaagact ttttagacga agatacacct ggtacaacac agacatccaa
      241 agaaacggat gcacataaat ggtttagttc aaaagaaaac cgaagcgatt ggaccatcac
      301 ttcaaaagac ggtctgaaat tatcagcgat ttatcttcct gctgaaacga aaagtgaaaa
      361 aacagctctt gtggctcatg gctatatggg aaacgctgaa acgatgacca attacgcaaa
      421 gatgtttcat gatatgggtt acaatgtcct tgttcctgat gcaagaggac atggaaaaag
      481 cgaaggggat tacattggat ttggatggcc agagcgtaaa gactatgtac agtggataaa
      541 caaagtgcta gaaaacaatg gaaaatcgca agaaatcgtt ctatacggtg tcagcatggg
      601 agcagcaaca gttatgatga ctagcggaga aaaactacca gataatgtga aagcaatcat
      661 tgaagattgc ggttattctt ccgttcatga tgagcttgct tatcagctag acgatatgtt
      721 tagtcttcca gcatttccgc ttatgcaagt aacgagtcta gtgacaaaag tccgtgctgg
      781 ctactttttc ggtgaagcaa atgcagtgga acaattgaag aaaaaccagc gcccaatgct
      841 ctttatacat ggagacgaag atacatttgt accatttgaa atgttagata aggtctatcg
      901 tgcaacgaag ggaccaaaag aaaagtatgt tgtaaaagga gccgagcatg cggaagcata
      961 caagacagat cctgaaaaat atcagcaagt agtccaacaa tttttaagcc aatatgttca
     1021 ttgaacgagt ttaaaagtag gggatgaaaa gaaagaagcc ttt
//
LOCUS       DARBDV010000213         1060 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000213 DARBDV010000000
VERSION     DARBDV010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.226"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(38..994)
                     /locus_tag="SCM56_002949"
     CDS             complement(38..994)
                     /locus_tag="SCM56_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111658.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          344 a          176 c          160 g          380 t
ORIGIN      
        1 tttagaaaaa gcagtcggac tttctaaaag aagttcacta ttcgatcctg tcgatgattt
       61 cttcaggaaa atcgtctatt tgcttgtcaa ttttgctgtc tttgtatttc tcttcttgga
      121 tagtcaacaa aatctttgta agattccccc agtctactgc tcttggcgga tcgagccaaa
      181 ccactacttg agatgcactt tcttcatata gattattcct attcgtgatt ataacatccg
      241 ggaactcagg ctttcctgcc tctatcactt ccgtgttcag attaatgtac aagcttaaat
      301 ttttcttgat aaattgatta taggcagcac cataagaaaa atcgatgatg atctttactg
      361 gttcagaaat cagttgaatg ggtatgtgat tgatcaataa taataaataa ctgaaaaaaa
      421 gaaaaaattt atcttgaagc aactctttgc gttttgacgt tagatcattt atatactcta
      481 tcaaatagca ataaaatgct ggatagtttt ctctaaaaaa agaaacatca agtggaaatt
      541 catcagcttt attaatagtg taatgcaaaa gtttaaaatg tattttggtt aactctcttc
      601 ggatcaattg gctcgtctct tctcgcaaca gattaaactg attttcaaaa ctttcaatga
      661 atcgttcatt caaccattcg attgtttcat cttcattttt tctcaagtat ttttgatcca
      721 atttatcatg aagataaagc caagagagtg tcttattgat aaaaataacg gatttttctc
      781 ctaatactcc ttttaagtgg taaaatccta tatccgaaaa aaatagcgac tgattgctag
      841 caatatctaa agaatcagct tgttcagcca acaggttgat tgctaaaaag tggaataatt
      901 tctttttttc ataagcagac agaggataat actgttttaa atcttttaac attttttctg
      961 tttttataat tataggtagc gtatacaatt ccacaaaact tatctctgtt gagttaaaaa
     1021 gattatataa aaaaagccga atgtttttag gattccctgt
//
LOCUS       DARBDV010000214         1051 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000214 DARBDV010000000
VERSION     DARBDV010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.227"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="SCM56_002950"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="SCM56_002950"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HEG1111659.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          377 a          132 c          182 g          360 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt t
//
LOCUS       DARBDV010000215          995 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000215 DARBDV010000000
VERSION     DARBDV010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.229"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(26..913)
                     /locus_tag="SCM56_002951"
     CDS             complement(26..913)
                     /locus_tag="SCM56_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEG1111660.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          321 t
ORIGIN      
        1 taatttcttt gcctttttat ctaaatcaaa cgtttagttc ttgaccaact aataaagaat
       61 aactggacat agacatgcca tttttcgcca ttagatcatc aacagacatg ccaaattttt
      121 gtgcaatatc ccatagcgta tcacctgaca cgactgtata tgtctgacca ttatttgtat
      181 ttacatctgt tgttgaagat gatgacgttg aagtcgtcgt agtagtcgtt gttgttgtac
      241 ttgtggttgt tgtagccgaa cctgttgatg gcgtatcaaa acgggttaaa ttataagtat
      301 taatcaatga gttcaatttg gccccataat ttggatcggt agcataacga cctgtcaagt
      361 acgctgttgc atcataaaaa cttgttgtat tgcttttcca cacgccagca taatgataat
      421 cgcccgtgga aaaactggtt gaacgaagaa cattcgcatg gtcttggaag gattcccaat
      481 aagaagaata ttttcggaat ggctcattca tcgttaccca ttgtccgttt acatattctt
      541 tggttgccat ataaacgacc tgtccgttat agcttccttt cacaccgaat agattataat
      601 ttggcgcact agctaatccg gaagtgccat atgcactttc caacaacgct tgagcgatca
      661 tgactgaagc atacaagtca ttacttgcag caacttctga cgcagaccag ccgatttgat
      721 cgataaacgc ttgaggatca gcgactgttt gagatccttg ttcatctgca tgaacatgtt
      781 gcggtaccgc tgtaaccatc atcggcccaa ctaaaagact cgttccaatt aaaggagcaa
      841 tcgctttttt attgatcacc atttttcttt taggtctatg gtgccgggca ctgcgggtct
      901 tcagctcttg cattcaaatt cctcctcaaa ataaaaaaac ttaataaaaa gttctcttct
      961 taaaacaaac ttaattaatt aaattatact tttaa
//
LOCUS       DARBDV010000216          994 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000216 DARBDV010000000
VERSION     DARBDV010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.230"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..>994)
                     /locus_tag="SCM56_002952"
     rRNA            complement(<1..>994)
                     /locus_tag="SCM56_002952"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DARBDV010000217          988 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000217 DARBDV010000000
VERSION     DARBDV010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.231"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..704
                     /locus_tag="SCM56_002953"
     CDS             <1..704
                     /locus_tag="SCM56_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEG1111661.1"
                     /translation="YSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAP
                     TESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIA
                     HKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFY
                     NFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRI
                     HKGFKEIQDTLESMI"
BASE COUNT          335 a          161 c          163 g          329 t
ORIGIN      
        1 attattccgt tatatttatg gatgcgacgc atatccctgt gaagcgacag actgtttcga
       61 aggaagcagt gtatattacg attggtattc gtttggatgg aaccaaagaa gttcttgggt
      121 ttactattgc cccaactgag tctgcttata tttggaaaga agttcttcaa gatcttagaa
      181 aacgtgggtt agaagaagtt ttattagtag tgacagacgg attaagctgt attgaagaaa
      241 gtatccatag tgtgtatccg aatgcccaat ttcaacaatg ttgtgtgcat gtatctagaa
      301 atatcgctca taaagttcgt gttcgaggtc gaaaagaaat ttgtgaggat ttcaaattgg
      361 tttaccaagc gaattcaaaa gaagaggcat tggatcacat cgactttatg actaggaaat
      421 ggaaaaagca gtatccaaga gtcgtcaatt tactcttgaa tcctgcccta ttaacctttt
      481 ataatttccc tcacgccatc agacgaacaa tttattcgac gaacctgatt gaaggcttta
      541 ataagcagct aaaacgatat actcgaagaa aagaacaatt ccctaatgaa gaatctctag
      601 agagattctt catttctcaa tttaatcaat ataaccaaaa atttttaggt agaattcaca
      661 agggatttaa agaaattcag gatacattag agtcgatgat ttaactgtaa ttaacagaat
      721 ggattttcca tttacatata attcttgacg ctaccataat ctaaataacc atcatattca
      781 tctactactt atataattga gttttattaa actttttaag tttctattct atcaaatatt
      841 taaacaatct ctgcatccga aaagactacc tcgtatagtt ttactaacaa tatttttatt
      901 agcgtgttca aatcctgatt tcttgtatca attattttct gttcactttc aataagaaaa
      961 aagtctttac ttccatggat aataataa
//
LOCUS       DARBDV010000218          959 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000218 DARBDV010000000
VERSION     DARBDV010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.232"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(107..511)
                     /locus_tag="SCM56_002954"
                     /pseudo
     CDS             complement(107..511)
                     /locus_tag="SCM56_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            complement(570..>959)
                     /locus_tag="SCM56_002955"
     CDS             complement(570..>959)
                     /locus_tag="SCM56_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1111662.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          287 a          170 c          159 g          343 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttccattggg agcgtcaata attttgtgta
      541 aataaattgt cctcctgcaa aataattagt tactcagtaa acattgaaac taatgtatcg
      601 gttacctgtt gaaaaccttt atggcttctg tttagaaatt tttgattgta tgtatcaaaa
      661 atgctgacta gaaagcgttc tagtgattct tcattttgaa actgctcttt tctacggctg
      721 tatcttttaa tttgcttatt gaaagactcg attagattgg ttgagtaaat ggttctacga
      781 atgctaggtg gaaaatcata aaaagttaat aagtcttggt tttctatgag tgactgcgtc
      841 actttaggat agtttttctt ccatttctca atcatgccgg ataagaaggt attcgcttct
      901 tcttttgagt tagcttgata aacagcctta aagtcatcac agatttcttt tcggtcttt
//
LOCUS       DARBDV010000219          931 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000219 DARBDV010000000
VERSION     DARBDV010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.233"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>931
                     /locus_tag="SCM56_002956"
     CDS             <1..>931
                     /locus_tag="SCM56_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEG1111663.1"
                     /translation="HCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFY
                     RPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFS
                     RFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETT
                     VRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAA
                     CPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNA
                     LHYSYSNGPLECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          286 a          190 c          180 g          275 t
ORIGIN      
        1 acactgtttc attagtcaaa aagttcgttg gtctgtcatt gctcgcttaa aagagaatac
       61 ttccatgacg gaaatcgctc gtcagaaaaa tatttccact tcttcggtct atcgtgtcat
      121 gaagcgattc taccgcccat tgaatccttt caaacagact ttgccaaagg tcttatgctt
      181 tgatgaattc aaatcggttc gaagtgttgc cagtgcaatg agttttatca tgatggacgg
      241 acaaagccat gcattattgg atattgtcga aaatcgacgc ttgccttatt tagaacgcta
      301 tttttctcgg ttttcattag cgactcgtga agccgttcaa ttgattgtta ttgatatgta
      361 tgcgccttat gtttcgttgg tgaaaaagct tttcccgaat gctcagttga tcatcgatcg
      421 tttccacatc gtccaacata ttggcagaac tttcttgaat catcgagtga aagaaaccac
      481 ggtacgactt aaaggaaatt cccactctca acgagggtta gggaaaaaat tgaagcgcta
      541 ttggaaactc ctacaaaaag aagaagcaaa gttaaactat gaaaaacggg tttggcgtgc
      601 cagttttaaa gattatttga ctgaaagtga gattgccgat cgactgttag ctgcttgtcc
      661 caacttacgc caaggctatc agttatacca agacattctt tatgcggtaa agaaaagaga
      721 tcagtcacta tttgaggatt gtttaactag agacattagc ggtctgccag aaacctatac
      781 aaccacttta cgaacgttta aaaagtttct gccacagatc caaaatgcgc ttcactacag
      841 ctattcaaac ggaccattgg aatgcttaaa caaccacatc aaagtattaa aacgcaatgc
      901 gtacggcttt cgcaactttt acaatttcaa a
//
LOCUS       DARBDV010000220          890 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000220 DARBDV010000000
VERSION     DARBDV010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.234"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..888
                     /locus_tag="SCM56_002957"
     CDS             <1..888
                     /locus_tag="SCM56_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HEG1111664.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DARBDV010000221          832 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000221 DARBDV010000000
VERSION     DARBDV010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.235"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>832
                     /locus_tag="SCM56_002958"
     CDS             <1..>832
                     /locus_tag="SCM56_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1111665.1"
                     /translation="SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHY
                     PKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIR
                     AYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDS
                     IEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEY
                     SYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISEC
                     T"
BASE COUNT          349 a           75 c          137 g          271 t
ORIGIN      
        1 aatcattaaa aaaacattat acagagcctt gggaagatga agtttttaga gtaattattg
       61 aatataacaa tgttcctatt ggatatggac aaatatataa aatgtatgat gagttatata
      121 ctgattatca ttatccaaaa actgatgaga tagtctatgg tatggatcaa tttataggag
      181 agccaaatta ttggagtaaa ggaattggta caagatatat taaattgatt tttgaatttt
      241 tgaaaaaaga aagaaatgct aatgcagtta ttttagaccc tcataaaaat aatccaagag
      301 caataagggc ataccaaaaa tctggtttta gaattattga agatttgcca gaacatgaat
      361 tacacgaggg caaaaaagaa gattgttatt taatggaata tagatatgat gataatgcca
      421 caaatgttaa ggcaatgaaa tatttaattg agcattactt tgataatttc aaagtagata
      481 gtattgaaat aatcggtagt ggttatgata gtgtggcata tttagttaat aatgaataca
      541 tttttaaaac aaaatttagt actaataaga aaaaaggtta tgcaaaagaa aaagcaatat
      601 ataatttttt aaatacaaat ttagaaacta atgtaaaaat tcctaatatt gaatattcgt
      661 atattagtga tgaattatct atactaggtt ataaagaaat taaaggaact tttttaacac
      721 cagaaattta ttctactatg tcagaagaag aacaaaattt gttaaaacga gatattgcca
      781 gttttttaag acaaatgcac ggtttagatt atacagatat tagtgaatgt ac
//
LOCUS       DARBDV010000222          820 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000222 DARBDV010000000
VERSION     DARBDV010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.236"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            136..309
                     /locus_tag="SCM56_002959"
     CDS             136..309
                     /locus_tag="SCM56_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111666.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
BASE COUNT          275 a          132 c          104 g          309 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc
//
LOCUS       DARBDV010000223          811 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000223 DARBDV010000000
VERSION     DARBDV010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.237"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..672
                     /locus_tag="SCM56_002960"
     CDS             <1..672
                     /locus_tag="SCM56_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1111667.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DARBDV010000224          781 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000224 DARBDV010000000
VERSION     DARBDV010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.238"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..737
                     /locus_tag="SCM56_002961"
     CDS             <1..737
                     /locus_tag="SCM56_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG1111668.1"
                     /translation="EVGELKQQINDLYSTLLRTIDDLEFKNKEILKLEKLKYDFFKGA
                     SHELKTPLASLKIILENMKYNIGKYKERDIYINECIEIVDSLTKNISQILSVHSIENL
                     NNDEEYLKINDTLEDILKKYEILANQKKITVNNYILDENVYIGKTALKIILSNLISNA
                     VKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIFDVKFDLNKENSNGLGLYIVSN
                     ILNNYNIEYKVLQDEEFFIFKIKIFS"
BASE COUNT          347 a           53 c          110 g          271 t
ORIGIN      
        1 atgaagttgg agaattaaaa caacaaatta atgatttgta ttctacttta ttaagaacaa
       61 ttgacgattt ggaatttaaa aataaagaaa ttttaaaatt agaaaagtta aaatatgact
      121 tttttaaagg tgcatcccat gaacttaaaa cccctcttgc tagtcttaaa ataatactgg
      181 aaaacatgaa atacaatatt ggaaaatata aagaaagaga tatttatatt aatgaatgta
      241 ttgaaatcgt tgatagttta acaaaaaata tttctcaaat attatcagtt cattctatag
      301 aaaatttgaa taatgatgaa gaatatttga aaataaatga tacattagaa gacatattaa
      361 aaaagtatga aatattagca aatcaaaaga aaataactgt caataattat atattagacg
      421 agaatgttta tataggaaaa acagctttaa agattattct atctaatttg attagtaatg
      481 cggtaaaata tactgatgta aatggggtaa ttaatattgg gatagttaat gattggttat
      541 atatagagaa ttcatacggt aacaataaaa tttcgaatat ggataaaata tttgatgtta
      601 aatttgattt aaataaggaa aatagtaatg gattaggttt atatattgta agtaatattc
      661 tgaataatta caatatagaa tataaagtat tgcaggatga agaatttttt atctttaaaa
      721 taaaaatatt ttcttaatta agatataatt ttgcaatagt tattgaaaaa tagagactta
      781 t
//
LOCUS       DARBDV010000225          763 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000225 DARBDV010000000
VERSION     DARBDV010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.239"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..339)
                     /locus_tag="SCM56_002962"
     CDS             complement(<1..339)
                     /locus_tag="SCM56_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1111669.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
     gene            complement(356..760)
                     /gene="tnpA"
                     /locus_tag="SCM56_002963"
     CDS             complement(356..760)
                     /gene="tnpA"
                     /locus_tag="SCM56_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HEG1111670.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          228 a          124 c          124 g          287 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgccaccag
      421 ttgtcagaag acaaaaactt ttagaccaga acatttcttt ccaaagaaat tgtttgactc
      481 ttggaaagtc acgttttatc aatcttgagc tggcactttt ataggcattg atgaattttg
      541 tcaattctgt tttaggttga gctttgaaca taatatgaac gtggtcttta tcatgattcc
      601 actccactaa agtaatgtga tacgattctg aaattctttc aaaagtagtt ttggcataat
      661 cagatatttc atcatcaatc acttgtctac gatatttcgt tactaaaaca aggtggtaat
      721 aaagaagaaa tactgaatga ttatttgtat ctaattccat gtt
//
LOCUS       DARBDV010000226          757 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000226 DARBDV010000000
VERSION     DARBDV010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.240"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            330..>757
                     /locus_tag="SCM56_002964"
     CDS             330..>757
                     /locus_tag="SCM56_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEG1111671.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKS"
BASE COUNT          288 a           84 c          125 g          260 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatc
//
LOCUS       DARBDV010000227          756 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000227 DARBDV010000000
VERSION     DARBDV010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.241"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(91..>756)
                     /locus_tag="SCM56_002965"
     CDS             complement(91..>756)
                     /locus_tag="SCM56_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HEG1111672.1"
                     /translation="RRRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQ
                     EQPLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYS
                     LTDVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVH
                     QYQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSG
                     EAA"
BASE COUNT          204 a          178 c          146 g          228 t
ORIGIN      
        1 ttgtgaatgc tgcggtgtta tggctacgat tgcccttgac aatgagcatc ctcggatctg
       61 attttctccg gcagaagcac aatcattcaa ttacgctgcc tctccagagg tatcagatcc
      121 gcgctcatta tcaaggattc gcttcgccaa tctctgttgt cgaatttgga tcggtgtttc
      181 atacgccaac gtgccatgta agcgtagata gttccaccaa tgcacataat caaacaattc
      241 tagacgtagc tgtgccagtg tctcaaattg gtattgatgc acgaattcta ctttgacaga
      301 tttataggtt gattccacaa cggcattatc atacggacaa cccttcctac tcaatgaacg
      361 agtaattcca aaaccattca gaatctcatg aatggtttgg ttatcaaatt cctttccccg
      421 gtctgtatga aaaagcttga cgtctgttaa agaatacggt atccgtccaa aggcttcttt
      481 taccaatgag gcatctttct tctcaccaca ggaataacca ataatttctc gattaaacaa
      541 atcaagtatt aagcagatat aatgccactt tttccccacg cgaacataag taagatccgt
      601 aacgatggct tccaatggct gttcttgtgt aaaggtccga tctaatacat tcgctgtttt
      661 cgcttcatta caagctgttc gttgcccttt aaaatgagcg atcgtatagg tagatgtcaa
      721 tccgcggcgt ttcatgattc gaccgattcg gcgccg
//
LOCUS       DARBDV010000228          753 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000228 DARBDV010000000
VERSION     DARBDV010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.242"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            153..>753
                     /locus_tag="SCM56_002966"
     CDS             153..>753
                     /locus_tag="SCM56_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111673.1"
                     /translation="MSVKKMLFMVSTIIVLTSRFWGINTSLSMRYFIMAIWDLYFMLS
                     YFSYREKTYKKYVIKNNFWLTIMPFVIFSIYTLIIWGIGSNAEFENFIKLCSTLLYLI
                     LAYGYACTAFYLFKAEGIDFIFWSGVISYMSGSVFYLIVSYGLNGLISYIKSLITGET
                     NTANFAMEVHDLTFAMGVFFLYYVFFENKNTKKHKIKIIV"
BASE COUNT          263 a           76 c          119 g          295 t
ORIGIN      
        1 agaaattaaa tatattgtat tgttacatta aagaaaactt gtatggaaaa atcagtacaa
       61 taccattatt taatgttctt cacttaaagc gcaaaaaata tcagtttatt aaactcgtat
      121 atagaacaaa tatatgtaga cggggcgaaa cgatgagtgt aaaaaaaatg ctatttatgg
      181 taagtacaat aattgtatta acatcccgat tttggggaat taatactagt ttatctatgc
      241 gatattttat tatggcaata tgggatttat attttatgtt aagctatttt tcttatcgtg
      301 agaaaacata taaaaaatac gttatcaaga ataacttttg gttgactata atgccgtttg
      361 tgatattttc tatctatact ctaataatat ggggaattgg ttcaaatgca gaatttgaaa
      421 attttataaa gttatgttcc acgttgcttt atttaattct tgcatatggt tacgcatgta
      481 cagcatttta cttatttaag gcagaaggaa ttgattttat tttttggtca ggtgttattt
      541 catatatgag tggttcagtt ttttatttaa ttgtatctta tggtttaaat ggtttaattt
      601 cttatataaa aagtcttata acaggtgaaa caaatactgc aaattttgct atggaggtac
      661 atgatttaac ttttgcgatg ggggtttttt tcctttatta cgtatttttt gaaaataaaa
      721 atacaaaaaa acataagata aaaattatcg tat
//
LOCUS       DARBDV010000229          733 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000229 DARBDV010000000
VERSION     DARBDV010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.243"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>733
                     /locus_tag="SCM56_002967"
     rRNA            <1..>733
                     /locus_tag="SCM56_002967"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          226 a          146 c          216 g          145 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga att
//
LOCUS       DARBDV010000230          731 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000230 DARBDV010000000
VERSION     DARBDV010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.244"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(196..>731)
                     /locus_tag="SCM56_002968"
     CDS             complement(196..>731)
                     /locus_tag="SCM56_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEG1111674.1"
                     /translation="DYPRINRNNTTAFKIFRAIINRCLRMIKLPPKIRHHKFDLYHGS
                     QWWALSKECLTELIQMYEQNQQDYLNFKIGMFAPDEKFFHTLFFNSSFKNKNVIGGPD
                     LPLELKNIEETTLQTSKLANIHIIDPSMNKWFDERDIDQIKESEKLFVRKVKSDYSDK
                     LLNRIDKEILKVGNIDE"
BASE COUNT          254 a          116 c           84 g          277 t
ORIGIN      
        1 aaacgcattt taaaaaaatt tttctttata aaaacctcat acaaaactat aattccaccc
       61 caagcaaaag ctattttcat cgggatatta gttacattaa aagtactagg cataaaataa
      121 agcagacaaa tgattaaatt tattactcga aacgcatctc gacttaataa actcttctct
      181 aaaacaggta ctattctatt catctatatt tccaactttc aaaatttctt tatcaattct
      241 atttaatagt ttatcagagt aatccgattt aacttttctt acaaataatt tttcactttc
      301 ttttatttga tctatatccc tttcatcaaa ccatttgttc atggaaggat caataatatg
      361 aatatttgct aatttcgaag tttgtaaagt agtttcttca atatttttca attcaagtgg
      421 taaatctggt cctccaataa catttttatt tttgaacgag gaattaaaaa aaagtgtatg
      481 aaaaaatttt tcatctgggg caaacatacc tattttaaaa ttcaggtagt cttgttgatt
      541 ttgttcatac atttgtatta attctgttag gcattctttc gacaaagccc accattgaga
      601 accatgatac aaatcaaatt tatggtgtct aatttttggg ggaagtttaa tcatcctcag
      661 acaacgatta attatagctc taaaaatttt aaatgcagtt gtgttattcc tattaattct
      721 aggataatca t
//
LOCUS       DARBDV010000231          712 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000231 DARBDV010000000
VERSION     DARBDV010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.245"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..446)
                     /locus_tag="SCM56_002969"
     CDS             complement(<1..446)
                     /locus_tag="SCM56_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1111675.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT          216 a          123 c           95 g          278 t
ORIGIN      
        1 aactttctct tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat
       61 actttataag ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata
      121 tatttttcct gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga
      181 gataacagag ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg
      241 acggttactc gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg
      301 atcgtttttt tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac
      361 tctgataaat ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct
      421 ttgcttaaaa aaaactcata attcattagt catcagctct ttctacgtat attactttcc
      481 atgcattcac caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca
      541 gcgattcttg tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt
      601 tttatttggc ctttctcgcg atcaaacctg ttattagaat tatatcattt actagtatgc
      661 atttttatta ataaaatggt atattttata ttgttataaa aagagcccaa cc
//
LOCUS       DARBDV010000232          703 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000232 DARBDV010000000
VERSION     DARBDV010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.246"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            133..>703
                     /locus_tag="SCM56_002970"
     CDS             133..>703
                     /locus_tag="SCM56_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HEG1111676.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT          248 a          101 c          128 g          226 t
ORIGIN      
        1 attttttgat tccccctgag tactattttt tataaatgct agtaaatcaa tcttctttgg
       61 cctttttttg ataaaaaaaa tactagatgt aagataaaag tgtaaaaacg atttgtttta
      121 ggaggggaaa cgatgtgtac cgcaatcact tatgcaacga aagaccatta ttttggaagg
      181 aattttgatt atgagatgtc ttataatgaa gtggtcacta ttactccaag aaattatcgt
      241 ttcgatttta gaaaggtaaa aaatttagat aaacattatg caatgatagg aatagcagct
      301 ggggtatcca actatccact ctattatgag gctacaaatg aaaaagggtt gagtatggca
      361 ggactgaatt ttcctggcaa tgctgattat aaagaattac aagaaggaaa agataacgtt
      421 gcaccatttg agtttattcc atggatacta gggcaatgct caaccataga cgaagctaaa
      481 gaattattgg ctactatcaa tctagtcaat atcgatttta gtgaaaaact acccttgtca
      541 cctttacact ggttattagc tgataaagaa aaatcgatag taattgaaag tatgaaagat
      601 ggacttcatt tatatgataa tcctgtcggt gtgttgacaa ataatccgcc atttgattat
      661 caattattta atctgaacaa ttatcgctcg ctatcaaatg gaa
//
LOCUS       DARBDV010000233          700 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000233 DARBDV010000000
VERSION     DARBDV010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.247"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(1..448)
                     /locus_tag="SCM56_002971"
                     /pseudo
     CDS             complement(1..448)
                     /locus_tag="SCM56_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
BASE COUNT          212 a          140 c          105 g          243 t
ORIGIN      
        1 cagacacttg cttaaagcga aaaatttctt caatgtccat gcctaattct ttggatattc
       61 tcttgtctga ccatccattt tttactaatc tttggatgac aatttccatt cctcttattt
      121 catgggtccc tcttgcacga ttgtgtcgaa ttgttgaagc catacgttct tatatgggtt
      181 tatcaataac tgttaccggt acaactttta agttgagtct ttccttagca acacgatacc
      241 tatgaagcac atcaaccaag atatacttct cttctttttc cttgtaataa caaactaatg
      301 gttgagtaaa gccatcttca atgatagaaa gctccaataa gttcatttcc ggagacgcta
      361 ctcgattggg attataatcg ttcgcctgta ttttggtaat tggaacaagc cgaacgtcca
      421 tcacagggaa agttatgtct ttattcatac gcttgtatac cctactttct taaaaagtag
      481 ggtatactca agaatccgat taattatctt ctcaatttgg ataatatatc tcatcagtct
      541 tcttgtcact ttatcaacat gattttgctg caaaatggga taccgacagt actggactgg
      601 taatcaggat aaaagcaaaa ataattaatt tgtatcctaa aaaatccata aaaattctct
      661 cacattttcg ggtattaatt cgagaatcac acctataaat
//
LOCUS       DARBDV010000234          635 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000234 DARBDV010000000
VERSION     DARBDV010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.248"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..270)
                     /locus_tag="SCM56_002972"
     CDS             complement(<1..270)
                     /locus_tag="SCM56_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111677.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFR"
     gene            complement(276..>635)
                     /locus_tag="SCM56_002973"
     CDS             complement(276..>635)
                     /locus_tag="SCM56_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HEG1111678.1"
                     /translation="RNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPP
                     RIDTKKLNMLETILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQC
                     ALMERAIAAKRKELDKK"
BASE COUNT          164 a          135 c          110 g          226 t
ORIGIN      
        1 acggaagtag aagaattcct tccagaagtc cgttggcatg cctgtatcga taaaaatatc
       61 gcctatcagc gcatagatga agtttcgttg ctgtacggtg aaacgtctag gatcaataaa
      121 acgaatttca atgactcgat caccatcgta gccgtcatac atcgtcttta gtcgatcaat
      181 gttcacttcc tcattgatgg ttgcgcttat gtcttttcct ttgaactttt tcagaaccgc
      241 tgaatatgaa tcgattaatg gtttaaacac tcatatcact tcttatctaa ttcttttctc
      301 ttagctgcta ttgctcgctc catcaaggca cattgctcat agcttaactg ttcaatagtt
      361 tcaacgttat cagctaagag ccctaattta tctgtctgct cattaacata ttcgattaag
      421 gttttggtca tatctttacc catctgctca ttgaaagctt ctagaatcgt ctctagcatg
      481 tttaatttct ttgtatcgat tctaggtggt gttggaatat cttccccttg aaatacatat
      541 aatcccagtc cgtgtagagc caatgctttc acaaagcatc gcttcaatga gttattgatt
      601 tgcattgcat ttggtttaac aactggttgg tttcg
//
LOCUS       DARBDV010000235          598 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000235 DARBDV010000000
VERSION     DARBDV010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.249"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
BASE COUNT          198 a          117 c           85 g          198 t
ORIGIN      
        1 gaaccggggg ctttattcct gcagtccggc tacgccttcc tgcaatagac aaaactccag
       61 atcgggtaat tcgcttgtgg cgaaacccga cctggagttt gtctctgtat tttgaaccct
      121 aaaaattaat caaacagact gatattctca taatcagcgc gaaataagcc attaagtaca
      181 gttttcctac ctatttttga taacccgctg gttagttaat attccttttc cgagagagat
      241 aaatgattgg ttaaatctat atacatctca acctttaaaa atgttttcga aatacttcat
      301 cagtacatgt catacaaatt accagtgcat tctcaaattc tagttcttta attgatttca
      361 gttgaggagc agttactttt ggcggaaatt tccatttttg tccaaacctc ttcccccgat
      421 gaaatttctt tgttgcaatt attgcaatga aaaatttttg tttcaaaaat atacataatt
      481 acttcctcca ataaaaacta tctacctctc aaattatttt tctctaataa gaaaaacaga
      541 aaaggtaata agatacaaca gtgatgattc ctaataaaga aaagactaca ataatatt
//
LOCUS       DARBDV010000236          567 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000236 DARBDV010000000
VERSION     DARBDV010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.250"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..287)
                     /locus_tag="SCM56_002974"
     CDS             complement(<1..287)
                     /locus_tag="SCM56_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1111679.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGR"
BASE COUNT          176 a           98 c           90 g          203 t
ORIGIN      
        1 cgcccgataa tgacgtcagg cgtccaacca agagtgactt tctctttaat gtaatctact
       61 tcatgagcag gtaattgaat gacttttcta ccacattttt tcttattttc tttatactgg
      121 tgccaataat caagagcagt cttgccttgc ttgaacttat tgactacatt ataaatagtt
      181 tgtatagcac gtcccattcg gttagcgatt tcaaccggct tattatgttg aagataatat
      241 gattctatca ttgtcagttc gtctatggta agatgtttgt aggtcattta tggttacact
      301 cctttgtttt ctttcgtcgg aaatacaatt tgagtgtacc ataaatgcct ttttattttt
      361 ctaacttaat tttacaattc gcgatatatt aaacaacgtt gtcgcgcttc attgaaacat
      421 tttatgaaaa ttccaaaaaa gagttgacac atgtttttag acgtgataag ataactaagt
      481 cgttaaaaag aaccacttgc gaagcttgat tctatcgaaa aaaaacttca aaaaagttct
      541 tgacaaataa caaacgataa gttatga
//
LOCUS       DARBDV010000237          553 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000237 DARBDV010000000
VERSION     DARBDV010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.251"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            129..527
                     /gene="spxA"
                     /locus_tag="SCM56_002975"
     CDS             129..527
                     /gene="spxA"
                     /locus_tag="SCM56_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEG1111680.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          196 a           86 c          110 g          161 t
ORIGIN      
        1 ttttaaaaaa attatgaaaa aaaggtagct atttaaaagt tgattatgta taatagaaac
       61 agatgggaga ttattatgat cttctgttac aaaaaagtga ttcttatcac gaaaaggagt
      121 ggtgcacgtt gttaacatta tatacttccc caagttgtac ttcctgcaga aaggctcgtg
      181 cgtggttgca agaacatcag attccatttg ttgaacgtaa tattttttca gaaccattga
      241 acagttctga attaaaagcg attttacaaa tgacagaaga cggaacagaa gaaatcatct
      301 cgacacgttc caaagtattc caaaaactga acatggattt agatgatctg ccgttacaag
      361 aattattgga acttgtgcaa aacaatcctg gattgcttcg ccgtccaatc atgattgatg
      421 ataaacgtct gcaagttggt tttaatgaag atgaaattcg tcggttccta ccgcgcgatg
      481 tacgacaatt agaattacgt caagcacaat tgatggctgg attatagaag gataatacga
      541 aacttggaaa aga
//
LOCUS       DARBDV010000238          526 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000238 DARBDV010000000
VERSION     DARBDV010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.252"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(6..359)
                     /gene="tnpB"
                     /locus_tag="SCM56_002976"
     CDS             complement(6..359)
                     /gene="tnpB"
                     /locus_tag="SCM56_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEG1111681.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(349..>526)
                     /locus_tag="SCM56_002977"
     CDS             complement(349..>526)
                     /locus_tag="SCM56_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111682.1"
                     /translation="IVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIYNGINNY
                     ILQAILKELLSNAL"
BASE COUNT          153 a          116 c           90 g          167 t
ORIGIN      
        1 ccagcttaaa aaactccttt ttttcctggc aaaatagctc gaggttgatc gatacttaat
       61 ccttctaaca accatcgaac ctgttggttc gttaaggttc gaacttcgtc tttcgtccga
      121 ggccatttta acttgccgtt ttctatccgc ttataacaga gaagaaaacc atcaccgtcc
      181 caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa ataaaaaaac ggcatcttcg
      241 taaagctcga gttgatactc ttgttgaata agactggcaa gaccatcaat tccttttcgc
      301 aaatctgttt ttccgcacac aatataaata tgtttggttt gaagtaaatc ataaagcatt
      361 ggacaacagt tctttcaaaa tagcttgaag aatataattg ttaataccgt tatagatcaa
      421 aacttcagaa ccattctctc ttttgatgcg catcgctaac ttggcagccg cgggtttacc
      481 tttttctggt gcactctttt cgtttcctaa ttggacagga acaatc
//
LOCUS       DARBDV010000239          521 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000239 DARBDV010000000
VERSION     DARBDV010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.253"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..160
                     /locus_tag="SCM56_002978"
     CDS             <1..160
                     /locus_tag="SCM56_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucocin A/sakacin P family class II
                     bacteriocin"
                     /protein_id="HEG1111683.1"
                     /translation="GIGTGIDVDAATYYGNGLYCNKEKCWVNWGQSWSEGLKRWGDNL
                     FGSFSGGR"
     gene            160..510
                     /locus_tag="SCM56_002979"
     CDS             160..510
                     /locus_tag="SCM56_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEG1111684.1"
                     /translation="MTSINIDHTKKVIHELYNSLMTRPNQTKALLDITDVLFQVYKKI
                     DTAKYPEILVNKLVNYIYIVGFDNKIRFIDNDEKLLIELGDISKKAGINSKYKANFTD
                     KSQFYSYSETMPQR"
BASE COUNT          208 a           57 c           92 g          164 t
ORIGIN      
        1 tggcattggt acaggaatag acgttgacgc agctacttac tatggaaatg gtctttattg
       61 taacaaagaa aaatgttggg taaattgggg acaatcatgg tcagaagggt taaaacgttg
      121 gggagataat ctttttggta gttttagtgg aggacgataa tgacttcaat aaacattgat
      181 cacacaaaaa aagtcataca tgaattatac aatagtttaa tgacacgacc taatcagacg
      241 aaagcattat tagatattac tgatgtttta tttcaggtat ataaaaaaat tgatacagca
      301 aaatatcctg aaattcttgt aaataaatta gttaattata tttacatcgt gggttttgat
      361 aataaaatac gctttattga taatgatgaa aaattattaa tagaattagg ggatatttct
      421 aaaaaagctg gaattaatag taaatacaaa gcaaacttta cagataaatc gcaattttat
      481 agttattcag aaacaatgcc tcaacgataa aatatagtat a
//
LOCUS       DARBDV010000240          514 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000240 DARBDV010000000
VERSION     DARBDV010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.254"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..132)
                     /locus_tag="SCM56_002980"
     CDS             complement(<1..132)
                     /locus_tag="SCM56_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEG1111685.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DARBDV010000241          513 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000241 DARBDV010000000
VERSION     DARBDV010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.255"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>513
                     /locus_tag="SCM56_002981"
     CDS             <1..>513
                     /locus_tag="SCM56_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HEG1111686.1"
                     /translation="QDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHTTIYRWVQEY
                     GKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWLRKKRDTQA
                     AYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLNNLIEQDH
                     RPVKRRNKFYR"
BASE COUNT          179 a           87 c          101 g          146 t
ORIGIN      
        1 gcaggatgtg attattgtag ccgtgggcta ctatcttcgt tataacctta gctatcgtga
       61 agttcaagaa atcttatatg atcgtggcat taacgtttct catacgacga tttatcgttg
      121 ggtgcaagaa tatggcaaac tactctatca aatttggaaa aagaaaaata aaaaatcctt
      181 ttattcatgg aaaatggatg aaacgtacat caaaattaaa ggaaaatggc attatttgta
      241 tcgagccatc gatgcagatg gtttaacctt ggatatttgg ttacgtaaaa aacgggacac
      301 acaagcagcc tatgcttttc ttaagcggtt agtgaagcag tttgatgaac cgaaggttgt
      361 agtcacagat aaagccccct ctattacaag tgcctttaag aaactaaaag aatacggctt
      421 ttatcaaggg acagaacatc gtaccattaa atacctgaat aatttgattg aacaagacca
      481 tcgtccagta aagagacgca ataaattcta tcg
//
LOCUS       DARBDV010000242          494 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000242 DARBDV010000000
VERSION     DARBDV010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.257"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..325
                     /locus_tag="SCM56_002982"
     CDS             <1..325
                     /locus_tag="SCM56_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1111687.1"
                     /translation="KASLKSLKDTWLDLSEDNPYSQFLITVMAGVNQLERDLIRMRQR
                     EGIELAKKEGKFKGRLKKYHKNHAGMNYAVKLYKEGNMTVNQICEITNVSRASLYRKL
                     SEVNN"
BASE COUNT          197 a           66 c           90 g          141 t
ORIGIN      
        1 aaaggcaagt ttaaaatcac taaaagatac atggcttgat ttatcagaag ataatccata
       61 cagccaattc ttaattactg taatggctgg tgttaaccaa ttagagcgag atcttattcg
      121 gatgagacaa cgtgaaggga ttgaattggc taagaaagaa ggaaagttta aaggtcgatt
      181 aaagaagtat cataaaaatc acgcaggaat gaattatgcg gtaaagctat ataaagaagg
      241 aaatatgact gtaaatcaaa tttgtgaaat tactaatgta tctagggctt cattatacag
      301 gaaattatca gaagtgaata attagccatt ctgtattccg ctaatgggca atatttttaa
      361 agaagaaaag gaaactataa aatattaaca gcctcctagc gatgccgaaa agccctttga
      421 taaaaaaaga atcatcatct taagaaattc ttagtcattt attatgtaaa tgcttataaa
      481 ttcggcccta taat
//
LOCUS       DARBDV010000243          485 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000243 DARBDV010000000
VERSION     DARBDV010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.258"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(62..259)
                     /locus_tag="SCM56_002983"
     CDS             complement(62..259)
                     /locus_tag="SCM56_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG1111688.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          158 a           89 c           68 g          170 t
ORIGIN      
        1 gaattttttc taccgacata aacaaaaaaa ccacctcata tgaggcggtt aacgttcaac
       61 attattttac gatatttact gcttgaggac cacgttgtcc ttcttcaata tcataagtaa
      121 ctgcttggcc ttcatctaaa gacttaaagc cttctccttg gattgctgag aaatgagcga
      181 atacgtcatt tccatcttca ccagtgatga aaccaaaacc tttatctgag ttaaaccatt
      241 ttactgtacc gttattcata tatatttcct cctgatacgt atataatacg tgttttattg
      301 caattaatat tacttggtaa aaggagtttt tatagtgtga atatatcgct cagattacgt
      361 ttcaaattag attacttatt tactataaca cggatgtaat gctaacacaa ggataatcac
      421 tcgatatacg gtttaaatat tattatttgt caaattaagc gagacaaaac tttccattta
      481 cgtaa
//
LOCUS       DARBDV010000244          465 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000244 DARBDV010000000
VERSION     DARBDV010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.259"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(31..>465)
                     /locus_tag="SCM56_002984"
     CDS             complement(31..>465)
                     /locus_tag="SCM56_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEG1111689.1"
                     /translation="RETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNH
                     LLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKA
                     KEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          155 a           82 c           26 g          202 t
ORIGIN      
        1 ttttataata gcttttcata tattatataa tcaatcttta taagtccttt tataaatttc
       61 ttttctacca ttttcgataa attcctgttt aatattttta attccataaa caatagtttc
      121 aataggataa tattcttcaa ctatatcttg atattctttt gctttctcaa tatctatatt
      181 tccatacatt cttaatatat cttctccaaa atttgttcct atttcttctt cactatcttc
      241 aagtaagtat ataaaatcac aatattcatc tataattcca gaatctccaa aatcaattat
      301 tccagttaat ctattattgc catctaacaa tagatgatta caactaaaat cattatggca
      361 taaacacttt ttaccctcaa aaactgttgt tgcatttagt ctttccataa aactttctat
      421 ataatctttt tctatatcag ttaaatcatt ataaatagtt tcacg
//
LOCUS       DARBDV010000245          462 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000245 DARBDV010000000
VERSION     DARBDV010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.260"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..430)
                     /locus_tag="SCM56_002985"
     CDS             complement(<1..430)
                     /locus_tag="SCM56_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG1111690.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DARBDV010000246          452 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000246 DARBDV010000000
VERSION     DARBDV010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.261"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>452
                     /locus_tag="SCM56_002986"
     CDS             <1..>452
                     /locus_tag="SCM56_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111691.1"
                     /translation="GKPNLMSKLKSSDFNVGYHGSLTSDNEKLHFTSDGTGSIIMFTR
                     INTPQLASGKTYTLSAKVRFDEGTTGAIDKLRLVYRTSPGEKILLEANSTNITTDDVG
                     KEITIKGTANVNYQITNLDRFYMSISFVDRDKINGGFKLYDIKIEEGST"
BASE COUNT          165 a           68 c           92 g          127 t
ORIGIN      
        1 ggtaagccga atttaatgtc caaactaaaa tcgagcgatt ttaacgttgg ttaccacggg
       61 tcactaactt cggataacga aaagctacat tttacttctg atggtacagg aagcattatt
      121 atgtttacgc gtattaatac acctcagctt gctagtggga aaacctatac tctgagtgcg
      181 aaagttcgat ttgatgaagg aactacagga gctattgata aattacgttt ggtgtatcgt
      241 acatcaccag gagaaaagat attattggaa gcaaatagta caaatattac aacagatgat
      301 gtagggaaag aaataacaat caaaggtaca gctaacgtta attatcaaat cacaaattta
      361 gatcgatttt atatgagtat tagctttgtt gacagggata aaataaatgg cggatttaag
      421 ttgtacgaca tcaaaatcga agaaggctca ac
//
LOCUS       DARBDV010000247          401 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000247 DARBDV010000000
VERSION     DARBDV010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.262"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
BASE COUNT          112 a           98 c           76 g          115 t
ORIGIN      
        1 attataaata actcataaaa aatattttga ctttcaaaat cacttttgct accagaagtt
       61 gaggcgaagt gttttaagca tagttgtacc ctagtttgac cgttaaaaat aaataacgat
      121 cgctagagtg caactatgtt tgtttgccct accgtcagcg tcggcttggc ctctcacatc
      181 cattaccgtc gggattatct ccgttacata cccttacaaa atacgccctg taagcattgc
      241 atgaaaccac cgtgcatttg caccgtattt ccgccctagc cgttcaaatc ggtcgctacc
      301 accattcaga aacacgtgcg gtaatggaag gtttggagga agctatccag accactaacg
      361 attgtcccta tcgtcgtacg tttcactcag atcaaggttg g
//
LOCUS       DARBDV010000248          345 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000248 DARBDV010000000
VERSION     DARBDV010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.265"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..321)
                     /locus_tag="SCM56_002987"
     CDS             complement(<1..321)
                     /locus_tag="SCM56_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEG1111692.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHL"
BASE COUNT           97 a           55 c           63 g          130 t
ORIGIN      
        1 taaatggata atagtggttt ccaatgtatc gtttgttctt ttgtaggctg gtaaagtttg
       61 ttgtttaaac tcaccattac gatctctagg tatttccaat gttaattcac catattcggt
      121 tttgattgat cgaaagtaag aaccgtttct cgaattacct gaattaaaac cagtgcgatc
      181 atatttttcg taatctaaaa aagccgttaa ttcagtccgt aggagtgtgt ttatcgcttt
      241 ttctaagtgc gaacggaata attcatttaa atcgccttta gtgactagag tttgcacaat
      301 ttctgtagta aaatcattca tagggaagtc ctcttttctg tgaat
//
LOCUS       DARBDV010000249          338 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000249 DARBDV010000000
VERSION     DARBDV010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.266"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            1..338
                     /locus_tag="SCM56_002988"
                     /pseudo
     CDS             1..338
                     /locus_tag="SCM56_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          142 a           59 c           64 g           73 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcc
//
LOCUS       DARBDV010000250          331 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000250 DARBDV010000000
VERSION     DARBDV010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.267"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>331
                     /locus_tag="SCM56_002989"
     CDS             <1..>331
                     /locus_tag="SCM56_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element ISS1D family transposase"
                     /protein_id="HEG1111693.1"
                     /translation="YLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIW
                     LRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKY
                     LNNLIEQ"
BASE COUNT          114 a           60 c           68 g           89 t
ORIGIN      
        1 attatctttg gaagaagaaa aatagacaat ccttctattc atggaaaatg gacgaaacct
       61 atatcaaaat taagggacgt tggcattatc tttatcgtgc aattgatgcg gacggcttaa
      121 ccttagatat ctggttacga aagaaacggg atacgcaagc agcctatgct ttcttaaaac
      181 gactccataa acagtttggt gagccgaaag caattgtgac cgataaagca ccttctcttg
      241 gctccgcctt tagaaagtta cagagtgtgg gtttatatac taagacagag caccgaactg
      301 tgaagtatct taacaattta atagaacaag a
//
LOCUS       DARBDV010000251          328 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000251 DARBDV010000000
VERSION     DARBDV010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.268"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            52..>328
                     /locus_tag="SCM56_002990"
     CDS             52..>328
                     /locus_tag="SCM56_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEG1111694.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCK"
BASE COUNT          104 a           60 c           63 g          101 t
ORIGIN      
        1 cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa gatgtctcat
       61 actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt cgaggaaaat
      121 tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg aactcttctg
      181 tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg
      241 gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac ttatctggag
      301 ctgaagaaac aacgatttaa atgtaagt
//
LOCUS       DARBDV010000252          321 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000252 DARBDV010000000
VERSION     DARBDV010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.269"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(69..321)
                     /locus_tag="SCM56_002991"
                     /pseudo
     CDS             complement(69..321)
                     /locus_tag="SCM56_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DARBDV010000253          308 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000253 DARBDV010000000
VERSION     DARBDV010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.270"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(76..>308)
                     /locus_tag="SCM56_002992"
     CDS             complement(76..>308)
                     /locus_tag="SCM56_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1111695.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           94 a           46 c           56 g          112 t
ORIGIN      
        1 atgttggtga agttcaccaa catcaaaaag tatagagccg atagaaggac tgaccttctg
       61 tctcagattt ttttgttatt tcacttgaat caattgattg atcaaaaaaa tcctgatttt
      121 catgttctca aatgatttaa atccataaga taatcgtttc agtgtcttga tatggggaat
      181 cttggcctct attttgccat tagaatacgg gtaaatcata gcattgtaaa taccttcttc
      241 atacgttaaa aggttttgaa gtttcgtgcg aagttgtgca tcaagtgttt caggaagatt
      301 ttttaata
//
LOCUS       DARBDV010000254          301 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000254 DARBDV010000000
VERSION     DARBDV010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.271"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            1..301
                     /locus_tag="SCM56_002993"
                     /pseudo
     CDS             1..301
                     /locus_tag="SCM56_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          119 a           60 c           57 g           65 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcagc
      301 g
//
LOCUS       DARBDV010000255          282 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000255 DARBDV010000000
VERSION     DARBDV010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.273"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>282
                     /locus_tag="SCM56_002994"
     CDS             <1..>282
                     /locus_tag="SCM56_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEG1111696.1"
                     /translation="QSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGK
                     KGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNC"
BASE COUNT           84 a           52 c           56 g           90 t
ORIGIN      
        1 aacaatcgaa gagacttttt cctgaaatca attgtgttgt cgctgatgct gggtataaga
       61 cacctaagtt tgttcacttt ttgactcatt tgaaccttcg accttgtttg ccttattcaa
      121 ggcccaaagg gaaaaagggg ctattatcaa aaaatgagtt tctctatgac gagtattttg
      181 attgttatat ttgtccgcaa gatcaaatgc tcgctttttc aacggttact cgagaagggt
      241 accgagaata caaatccaat ccaaaagaat gtgtgaactg tc
//
LOCUS       DARBDV010000256          270 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000256 DARBDV010000000
VERSION     DARBDV010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.275"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>270
                     /locus_tag="SCM56_002995"
     CDS             <1..>270
                     /locus_tag="SCM56_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG1111697.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGW"
BASE COUNT           83 a           56 c           53 g           78 t
ORIGIN      
        1 atccatcgtc gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa
       61 tttaaatacc atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat
      121 ccttttatgg atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat
      181 gcattagcgg taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat
      241 cgtcgtacgt ttcactcaga tcaaggttgg
//
LOCUS       DARBDV010000257          239 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000257 DARBDV010000000
VERSION     DARBDV010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.277"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>239
                     /locus_tag="SCM56_002996"
     CDS             <1..>239
                     /locus_tag="SCM56_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG1111698.1"
                     /translation="AIIALKPEGRKAVDIENSINEWLQSVPKNLFKSITFDCGKEFSN
                     WKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRK"
BASE COUNT           84 a           46 c           43 g           66 t
ORIGIN      
        1 gccattatcg ccttgaaacc agaaggccgt aaggcagttg atattgaaaa ttcgattaat
       61 gaatggcttc aatccgtacc caaaaatctt ttcaaatcaa ttacctttga ttgtggaaaa
      121 gaattctcta attggaaaag tattagtaat acgaatgata ttgatattta tttcgcagac
      181 ccaggaacgc cttcccaacg gggattaaat gaacattcaa acggactcct ccgaaaaga
//
LOCUS       DARBDV010000258          227 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000258 DARBDV010000000
VERSION     DARBDV010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.278"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..>227)
                     /locus_tag="SCM56_002997"
     rRNA            complement(<1..>227)
                     /locus_tag="SCM56_002997"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           48 a           77 c           45 g           57 t
ORIGIN      
        1 atcgtctgca ttccttagcc tcctgcgtcc ctccattgct caaacaaaaa caacgggtac
       61 aggaatatca acctgttatc catcgcctac gcctgtcggc ctcggcttag gtcccgacta
      121 accctgggcg gacgagcctt ccccaggaaa ccttagtcat tcggtggaca ggattctcac
      181 ctgtctttcg ctactcatac cggcattctc acttctaagc gctccag
//
LOCUS       DARBDV010000259          224 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000259 DARBDV010000000
VERSION     DARBDV010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.280"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>224
                     /locus_tag="SCM56_002998"
     CDS             <1..>224
                     /locus_tag="SCM56_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEG1111699.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAA
                     ESQTPAKPAAEPTVDTESAGSSSEGVFQFK"
BASE COUNT           70 a           47 c           58 g           49 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agtt
//
LOCUS       DARBDV010000260          219 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000260 DARBDV010000000
VERSION     DARBDV010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.denovo.281"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            71..>219
                     /locus_tag="SCM56_002999"
     CDS             71..>219
                     /locus_tag="SCM56_002999"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEG1111700.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYS"
BASE COUNT           67 a           36 c           41 g           75 t
ORIGIN      
        1 tttatggatg atgtctacag tcttttcttt tataatagat ttatgaaaac gaatgcaagg
       61 agtgatagct atgatgtcta aaaacaatac gaatggtaga aatcaatttg cgatgcttac
      121 gattgatgat ctagttcctc aagatcatct cgttcgaaag attgatgccg cgcttgattt
      181 tgaatttatt tatccaatcg tcgaagccac ttactccga
//
LOCUS       DARBDV010000261        14000 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.guided.074, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000261 DARBDV010000000
VERSION     DARBDV010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.guided.074"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..743
                     /locus_tag="SCM56_003000"
     CDS             <1..743
                     /locus_tag="SCM56_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HEG1111701.1"
                     /translation="FLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDE
                     PAIDEIYHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYK
                     QEFIKRDVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFE
                     SMYFQKKLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDA
                     ENWAKRFITNDSTIYEQYEYDLSSVENK"
     gene            complement(893..1834)
                     /locus_tag="SCM56_003001"
     CDS             complement(893..1834)
                     /locus_tag="SCM56_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEG1111702.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            1960..3081
                     /locus_tag="SCM56_003002"
     CDS             1960..3081
                     /locus_tag="SCM56_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEG1111703.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3339..4841
                     /gene="eat(A)"
                     /locus_tag="SCM56_003003"
     CDS             3339..4841
                     /gene="eat(A)"
                     /locus_tag="SCM56_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HEG1111704.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            4869..5435
                     /locus_tag="SCM56_003004"
     CDS             4869..5435
                     /locus_tag="SCM56_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEG1111705.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            5646..6707
                     /locus_tag="SCM56_003005"
     CDS             5646..6707
                     /locus_tag="SCM56_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEG1111706.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            6710..7606
                     /gene="murQ"
                     /locus_tag="SCM56_003006"
     CDS             6710..7606
                     /gene="murQ"
                     /locus_tag="SCM56_003006"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEG1111707.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7620..9602
                     /locus_tag="SCM56_003007"
     CDS             7620..9602
                     /locus_tag="SCM56_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1111708.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9667..10113)
                     /gene="nrdI"
                     /locus_tag="SCM56_003008"
     CDS             complement(9667..10113)
                     /gene="nrdI"
                     /locus_tag="SCM56_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEG1111709.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            10324..11748
                     /locus_tag="SCM56_003009"
     CDS             10324..11748
                     /locus_tag="SCM56_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1111710.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(11929..12702)
                     /locus_tag="SCM56_003010"
     CDS             complement(11929..12702)
                     /locus_tag="SCM56_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEG1111711.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(12699..13604)
                     /locus_tag="SCM56_003011"
     CDS             complement(12699..13604)
                     /locus_tag="SCM56_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1111712.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(13758..13994)
                     /locus_tag="SCM56_003012"
     CDS             complement(13758..13994)
                     /locus_tag="SCM56_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QacE family quaternary ammonium compound efflux
                     SMR transporter"
                     /protein_id="HEG1111713.1"
                     /translation="MNYSFYTVIGMIASFYFLSKAIKSLPMSLSYPIWTGIGAVGSVI
                     IGVVLFHDKLNLSTWFFVGMLLISIIGIKITSGH"
BASE COUNT         4450 a         2490 c         3003 g         4057 t
ORIGIN      
        1 tttttttaga ttatggattt gatatggaga tggctcgacg cgtgcgccca tttaccaatg
       61 caaaaattgt gttatttttt tggaatcact tcaaagcaga acacgaggcg atgctgaagc
      121 aggcacaaga tgaaccggca atcgatgaga tttatcattt tgattttttg gaagcaaaag
      181 aacttgggct gaaacataat agttcatttt attcgaaaca tatgaatttt tcttctagcg
      241 aaccaaagac ggatctattt tttggtgcaa cgaataatgg gagaaaagaa cgagcagaaa
      301 gctataagca ggaatttata aaaagagatg ttacgaccaa ctatttcatt cttccacatc
      361 gagggaatga acaacctgga tatcttactt atgaagagta cctgaaaaaa acaggggaat
      421 cgcgcgggat tctggaactg ttgcgggagg gtcagcaagg agtgactttg cggacatttg
      481 aatccatgta ttttcaaaag aaactagtga cggataatca agcgattgct cattatcatt
      541 tttatcgtcc tgaaaatatt tttttgcttc gagaacgtga tttagatgaa ttaccggaat
      601 ttctgcatac accatttaag ccaatcctat cagagctatt ggaattttat gatgcagaaa
      661 attgggcaaa acgatttatc actaacgatt ccactattta tgagcaatac gaatatgacc
      721 tctcctctgt tgaaaacaaa taaaaaacct acattttgaa gagattatgt actgaaacaa
      781 acaagcacaa ttcttatggc atcatgagag ttgtgttttc tttttgatct tttattgggc
      841 ttaaaaaaag agaaccagct gctgattcag ctgattctca tcagagagcc ttttattctg
      901 cgccaaggat tccttctttt gcatggacta attgggtcaa aaaggcacaa tatggtgtag
      961 caatatcata tttttgtcct tttcgccaaa tggcaccatt gatgtagtcg atttcagtca
     1021 aacgatggtt tttgatcagg tcttggtaca tagatgggta atgcaacccg atgttgtcag
     1081 gatcataagt agaagcaata tgttggtaca cttcttcttg atctagtatg attccttctt
     1141 ttgctgcgat agaagcaaat tcagatacga ttgttcgaac catgctctct gccgcttttt
     1201 gcgcgcctag ttcagcaata ttacaatcca aaatcgtgca taaaccattc aatgttccat
     1261 ttacacaagc ttttcgccaa atggaataac gaacgttatg gctatagatc ggatttaaat
     1321 ttgcttcttg aaagacttct acaacttttt ttgtaaatgc ttcgccttct ggttccaagt
     1381 tctctagttc gatttctcca tcacctaaca atgtgacttt tccgggacca gctagaccag
     1441 ctgtccacat cgtgatccca aataagatat ttttctttgg cacatatttt tccagtacat
     1501 cttcatgacc taatccattt agtaaacaca aaacataagt attgtcagta ataatggatt
     1561 ggatcgaaca aaacatatca tctaattgat tggcttttgt taatgctacg atcaagtcca
     1621 cgtgttcgtt gctttcgatg atttcttctg gtgaatagat tggcagtttt gcgaccactt
     1681 ctttaccgtt aaagtcagca atcaacccat ttgtacgaat ggcttcaatg tgagcaggcc
     1741 aacgatcgat caagaggacc tcatttccgc tttgatgtag catcaatccg attcggctgc
     1801 ccattgctcc ggccccagct attgcgattt tcatgttttt tctctccttt attcaatgag
     1861 ttccctttta gtatatagaa aaatggaaga aaatagcatt gttttgtttg aaaaaaatga
     1921 aaaaaagtgt cttacttaag aagaaagtga gggatacaga tggatatttt ggcggcaatt
     1981 gattcattta aaggaagtgc ttcttcattt gaactaaatc aggcagttct aaatggctgg
     2041 gaagatcgta aaggaagaaa aatcaatgtg ccgattgcag atggcggaga aaatactgca
     2101 gaagcaatct attacgcgct caggggaaca tggcgtacca ttgatgggat ggatctctta
     2161 ttccgtcagc gaaaaatcag ctatttattg acaacttggc aaggaaaaaa caccgcagtc
     2221 atcgaatctg cacaagcatt ggggttagat ctacttgata aaccaacgga tcaaactatc
     2281 cgacttgctt ccagttatgc cttaggagaa cttattaaag atgcactgct tcaagaagta
     2341 gaacaaatta ttgtcacttt aggaggaagc gggtgtagcg atggtggtct aggattactc
     2401 caatccttgg gtgcttcttt agaagggtac acagaaggaa atcctttatt atctactaaa
     2461 aaacttcatt ttgatcgaag aaacgttctt gtttctaaag taaaattgat tatcgctgca
     2521 gatgttacca atccatatac aggtcaacaa ggagctgctt ggcaatttgg aagacaaaaa
     2581 ggaggaatag atacgacgct agctttctta gataaacaag caggaaagat tgctcagcaa
     2641 ataaaagatt tctgggaggt ggatttagat gatattccag gaagtggagc agcaggcggt
     2701 attggtggag cccttttttt gttgggagcg aagatggttt ctggtttcga tttagtcagc
     2761 caaatcactg gattggaaaa aaagatcgaa caagcagatg tcatcattac aggagaaggg
     2821 cggatcgatc agcaaacgat ccacggaaaa gttccatacg gtgtagcagt gcttgcaaaa
     2881 aaatatcgaa aaccaataat cgctatttgc ggaagtagag accaagagct agaaggactt
     2941 tctgattttc tcccaattgt cttaagtatt catttgggtc caataggttt agaagaagca
     3001 atggaacaca ggcaaacatt agaaaaaaca aaaatcttag gacagacact tagcagattt
     3061 ttcaccatcc tcccgaagta gttgacaaaa tcattttctt tatgctatcg taatttcaaa
     3121 tataggacaa agtgagggat ccgtgcattg aaaaattaag tcgattgatg aaagctgcat
     3181 tcagactgta ccttgtggag acaaggaagc agtgtgcaga ttttcgggat tggcttggtt
     3241 ttttcgatgt atgggtcttt ttgtgtgtat ccaacccttg catcctcttt agccagttct
     3301 gaaaatagga ctggtctttt tttacgaggt gatggaaaat gtctaaaatc gaaataaaaa
     3361 atctgacatt cggctacgac agccaaggca cattattatt tgaacaagca aatctaaatt
     3421 ttgacacaca atggaaacta ggacttatcg gacgaaacgg tcgaggaaag acaactttac
     3481 tgaatattct acaaaacaaa ctaccttatc aagggcaagt aatccatcag caagaatttg
     3541 cctatttccc gcaacagaca aaagataaag aacgtttaac ctattacgtg ttaaatgata
     3601 ttacggattt tgagatatgg gaaatcgaaa gagagctcca attgatgcaa acagatcctg
     3661 aaatcttatg gagagaattc agcacactat cggggggaga gaagacaaaa gtcctactgg
     3721 cacttttatt tgtggatgac actcatttcc cgttaatcga tgaaccaacg aatcatttgg
     3781 atatctctgg tagaaaacaa gtagcggctt atttgaaaaa gaaaaaacaa ggcttcatcg
     3841 tggtcagcca tgaccgggga tttatcgatg aagtagtgga ccatgtttta gcaatcgaaa
     3901 aaagtcaact ggaactttat caagggaatt tctctatcta tgaagaacag aaaaaacttc
     3961 gtgatgaatt tgaaatggct caaaatgaaa aattgaaaaa agaagtcagt aggctaaaga
     4021 aaacagcagc tgaaaaagcc gaatggtctc gttcccgaga aggagataaa acaaagaaac
     4081 aagtcggatt catcgatact gaatctagac gagtgaataa aggagcagtg ggtgctgatg
     4141 ctgcacggac gatgaaacga tccaaagcaa tcgtgaatcg gatggagacc cagatcagcg
     4201 agaaagaaaa actattaaaa gatatcgaat atatcgattc tttgacgatg aatagccaag
     4261 cgtctcacca taagcgactt ttaagcgtag aagatcttca attagggtat gaaaatctgt
     4321 tattcgagcc aattcatttt acaatcgagc ctcatcagcg ggtggcgatt tcaggtccta
     4381 acggtgcagg aaagtcatcc attatccatt atcttctggg ggcattcaac ggcaaggtta
     4441 taggagaaaa ataccagcca aaacatctga gcattagtta tgcaagccaa aattatgaag
     4501 acaatcgagg aacgttggcg gaatttgcag agaaaaacca agtagactac caagcatttt
     4561 tgaacaacct ccgaaagctt gggatggaaa gagatgtttt tcataacaag atcgagcaga
     4621 tgagtatggg ccaacggaaa aaagtggaat tggctaaatc tttatcacag ccagctgaac
     4681 tatatacatg ggatgaacca ttgaattatt tggatgtctt caatcaagaa caattagaac
     4741 aactgatctt gaacgtgaaa cctgccatgt tactagtgga acatgatcaa accttcctgg
     4801 ataaagtatc tactgagatt atttctcttg agagaatcta acccttgtaa tctcaagaac
     4861 aacaaggtat ggttaagggg aactttgcca acgaaggaga gaatcacatg ctttatgtaa
     4921 cgagacacgg agaaacaact tggaacgcac aaggattagt ttgcggacgg gcagatgtgc
     4981 cgctgactga aaaaggacag atgcaggcac agaaactagc agaaaaggta gtagatttgc
     5041 ctgttccaat cacgaaaatc atccattccc ctcttcaacg agcaagagat acagcacaag
     5101 cagttgctga tcgtctcagc ttgccgctga ccgttgatga acgattggta gaaatggatt
     5161 ttggggatta tgatggactg ccaagcaaag atgagaactt tcaaaaagca cgtttagcgt
     5221 tcgctgtacg attcccaaat ggggagtcag tcttagatgt gtatgcacgg atcgttcctt
     5281 tattgaaaga atgcatcgaa gacgaagaaa atgtctatct gttagtttgc cataatgcct
     5341 tgataagagt gatcaatgct tattttcatc cgatgcctaa tgaggggttc tttacattca
     5401 tggtagataa tacagaattg gtttcttatg agtaattatg tttttgtgtt atttaaataa
     5461 aatatttcaa ggcactgctt ttgcttagca gtgccttttt taggttatga caattctttt
     5521 gataatataa actcaacctt tagaaatgaa atttaattat tttaataaaa gtattgaaat
     5581 tttgtttctt tagatatata ctctagtcat agaaatgaag acggatacag aaaaagaggg
     5641 ataatatgtt tggcttttca atatttatga acgattcttt gtcagaagag aagaaacagt
     5701 atatccgcca aatggcatcc aatggctttg tcggtatttt cacttccatg catataccag
     5761 aagatgatgt ttcggcatat caaaagcgat tgatcgaact aggaagcgaa gcgaaaaaga
     5821 ataaactaga attgatggtc gatatttcag gagaggcatt ggaacgagcg ggtttttcat
     5881 tagaagatct aacgcctttg aaagcaattg gtgtaacagg tttaagaatg gattatcata
     5941 tttccaatca gcagattgcc gactggtcac atcaattgaa aatcagtttg aatgctagca
     6001 cgatcacgcc taaagatatc gatgaattga aagaagcaga agctgatttc tcacagattg
     6061 aagcttggca taattattat ccacgtcccg aaacagggct ggacaaagaa tggtatcaga
     6121 aaaaaaatca atggctcaaa attcaaggtc tccttgtcca aggttttgta ccaggagata
     6181 cagagttgag aggcccacta tatcaaggat tgccaacttt ggaagaacat cgagggatcc
     6241 atcctcttgc aggcgctcta gatctattag caagtaacac ggatattgtt tacatcggag
     6301 atgcaggtct gtcagaaaac gttcaggagc aatttgcttc tttccaaaaa gaacagacag
     6361 tcttgctcca tacggaacct gtagatgaag aattttacga atatatatta gggaagcata
     6421 cgaatcggca agacgatgca agagatgtca tacgaagcgt agatgcacgt tttcgagaga
     6481 ttccgcctat cccagcaaga aatacagcta cgcgtatgaa agggtctatt actttagata
     6541 acgaaaagta tttgcgctat atgggtgaga tccaattaac caaatatgat ttgccagcgg
     6601 atgaaaaagt gaatgtagta gctaaagtta tcaaagaaga gctaccattg atcaaccaaa
     6661 tcaaagctgg tatgaactat caatttataa gaaaagaagg aagataaaaa tgagcaaaat
     6721 caatttagag aacctaacaa cggaacgcag aaaccaagaa acttttggat tagatgagat
     6781 gagtatttct caagccgttc aactaatgaa caaagaagat cagaaagtgg ccgctgcggt
     6841 tgaaaaagaa ctgacaagta tcgaaccagt gattgctaac acgattgagt cctttaaaaa
     6901 aggtggacgt ctgatttata tgggagccgg aacaagtgga agactaggtg tgttagatgc
     6961 tgctgaatgc gtaccgacat tcggtgtaga accggaaatg gtcataggtc taattgctgg
     7021 tggagaagaa gcaatgactg tagctgtcga aggggccgaa gattcttatg agctagggca
     7081 agaagatcta atcaagctga atttgacaaa aaatgatata gttattggta tcgcagctag
     7141 cggtcgtaca ccatatgtga tcggtggatt agattacgca aaatcgatcg gtgctgtcac
     7201 aggaacgatt tcttgcaata aacaagccga aatcagtaaa cacgcggatt tcccaatcga
     7261 agtcgatgca ggtccagaat ttttgacagg ctctactcga ttgaagtcag gaacagcaca
     7321 aaaacttatc ttgaatatga tttctactat cagtatgatt ggtataggta aagtattcaa
     7381 taacttgatg gtcgatgtca aaccaacaaa tgaaaaacta atcgaacgct caaaacgaat
     7441 catcatgcaa gcgacagaag ctgattacga aacagctgaa aagtactttg aagaagcaga
     7501 tcaaaatgtt aagctggcta tcgtcatgat tttgacaaat agtgataaag aggaagctca
     7561 agcaaaatta gctgaagcaa acggatttgt aaaagggacc ttatgagggg gaaaagacca
     7621 tggcaaaaaa cgaactgact ttggaagaac tggcaaaaca aatttataaa ggggcaggcg
     7681 gcatgggcaa tgtagaaagc attgtccact gcatgactcg tgtgcgtatg aagatcatca
     7741 atgatgatct tgtaaatgtc tctgaattaa aagcgattcc aggagtgctt ggtgtagtag
     7801 aagatgaaca gcttcaagtc atcatcggac ctgggaaagt gaacaaagta gcacagcaaa
     7861 tggttgattt ggcaggcgtg aaattaggag aaaaactttc ttctgtaagt acagctacag
     7921 caaattcatc agcatctgga aaagaccgtg taaacgaacg ggcacaagaa atgaaagcgg
     7981 cacaaaaatc gaaacaaaaa ccatctaaaa tcaaatcggt attaaaagac atttctaata
     8041 tctttgtgcc aatgattcct gcatttgtcg gtactgggat cgtggcaggg attgccgcag
     8101 ttttagcaaa cttagtcaca gccggaacac ttgatgcagc aacatggcaa caatatatcg
     8161 atgtgatgaa catcttgaaa aacgggatgt ttagctacct agttatctat acaggtatca
     8221 atgctgcaca agtttttggt gcaacaccaa ctcttggtgg agttatcggg gccgttgtga
     8281 tgctgacagg gatgaatccg gaagcaccac tgaaaaactt atttactggt gaagctcttg
     8341 cagctggaca aggtgggatc ctaggtgtca tcttcgctgt ttggctattg tcaatcgttg
     8401 agaaaaaact gcataaagta gtacctgatg cggtagatat catcgttaca ccaactcttt
     8461 cattgattgc catcggtctg attgaaattt tcttcatcat gcctttagca ggtttcattt
     8521 ctgatggcat ggtcggtggg atcaactggg tacttggcgt tggtggagca tttgccggct
     8581 tcgttctagg cacattattc ttgccgatgg ttatgttcgg actgcatcaa attttgactc
     8641 caatccatgt gcaaatgatt gatgaaactg gtcgtacgct attgcttcca attcttgcga
     8701 tggctggtgc aggacaagtg ggtgcagcat tggcattatg gattcgctgc aaaaaagata
     8761 aagaattaac agaaatgatc aaaggagctt taccagtcgg gatcctagga atcggcgaac
     8821 cattgattta cggagtaaca ttacctctag gacgcccatt tattactgct tgtatcggtg
     8881 gaggtatcgg cggggcagtg atcggaatga tcggaaatgt cggggctatc gctattggac
     8941 cttcaggagc cgctttgatc ccattgatct ctgacggtaa atggttaggc tacatccttg
     9001 gattattggc agcttatgct ggtggcttcg ttgctacatt cttcttcggt attcctaaag
     9061 accgtttaga aaaagaagaa ttagcgactg aaacagccac agtaaatgaa gtgcctgaaa
     9121 aagtagtacc tcaaacttca gctgaaagtg tgactatcct ctcatcagtt gctgacggaa
     9181 cagttgtttc tttagataaa gcaagtgatc cagtttttgc acaaaagatg atgggtgaag
     9241 gctactttgt agaaccagaa aacggacata tctattctcc agttactggg gtagtcagct
     9301 ctgtcttccc aacgaaacac gctattggga tcacaacgcc aaatggttta gaaatcttgc
     9361 ttcatatggg aatcaacaca gttgacttag gcggtacgcc atttgatgtg aaagtaacag
     9421 aagggcagca agtgacacct gatacgcttg tagcagaagc tgacttagaa gcgatcaaag
     9481 cagcaggcaa agaaacatcc atgatcgtct tggtaaccaa tatggatcgt gtaaaaaact
     9541 ttgtcttgga aaaaacaggt aaagcaaaag caaaagcacc tgtgatggat gtcaaagctt
     9601 aatatgatag aaacaataga aacgaaagaa gaaactgaaa tctatttttc agcttcttct
     9661 tttttattat ttattgtttt ctgctaaaat ctgataaatt cgtgtcacat cagatggtgt
     9721 tccccgtaat tcataatctg ccaagaaagg aaagccaaat gtttgtgcat attgtttggc
     9781 tgttagacaa tactgattat tgaagttttt atttccgcta ccgactacac caagacaaag
     9841 tttatgattg tcttcatgtt ccagatattc acgcatcgtt tcggtcaaga tttctgtatc
     9901 accgttatct aaaccatttc cgccgtctaa ataagtagga acgaaagtaa aaaatggttc
     9961 tgtttctttc gtaaaatcac tattttcatg gatttctttt aaagtaactt caggattgac
    10021 aggatcttct gcatgcattt tttcagcata ttctgctaga tgtttggcaa aggcacgcgt
    10081 atttcctgaa atcgatatat ataaaatgtt catggatcat cctccaattc tttcctttta
    10141 gtatagcatc ttttttaagt tttttggtgg tgtaatttgg aaagggacta ggcgctttgc
    10201 ttgtataatg agaaaaaagc cggtagaatt atatttctat taaagttttt attaattaag
    10261 taaaaataaa aaaggtttgt tatacttttt gataaaagcg gttttatttt ggaggatgct
    10321 tacatggata cagagcaaca gaaaaaagga attggtttaa ttccacttgc tgccttagta
    10381 attggttcag cgattggagg tggcgtattt ggtattatga cggacatctc cggatcggct
    10441 ggcggcccag ctattttcag ttgggtactc gtaggaacag caatgttgat gctctccttg
    10501 tcgatcaata atatcaataa aaaacgtcca gatttaaaag gcgggatctt tagttacgca
    10561 gaagaaggtt ttggccgttt cgctggtttt atcagcggtt ggggttactg gctcacatgc
    10621 tggttaggca acgtagcttt tgcaacattg ttgatgagcg cattaggata tttcttccca
    10681 gtatttgaag gtgggcaaaa tctggcatcg atcgtgatga gtacagtctt tttatgggga
    10741 tatgcatggc tagtgaatcg gggagtcgaa aatgctagtt tcgtcaatgc tatcgtcaca
    10801 ttctgtaaat tagtgccatt atttttattt attgtagtag ctatcagcac gttttcgatc
    10861 caacaattca ttgataactt ttctagcgac attatattga atgcgaatgg acagccgatc
    10921 ccgttttcca gtcaagtatt gagttgcatc atggtgatgc tttgggtatt tgttggtatt
    10981 gaaggagcct ctgtcgtagg ttcacgagca aaaaaacaat cagatgttgg gaaagcaacg
    11041 attctaggga tattcggctt gattattatt tacgtcttgg tttccatgct tccatatggg
    11101 acaatgacga tgaatcagct gcaaacgatc aaggcgcagc ctgctatggg ctatgtgttt
    11161 gaaatgatgg ttggtaaatg gggagcagtc gtgatcaacg gcggtttgat catttcttta
    11221 gttggtgttt ggctctcatg gacgatctta ccgattgaaa caatgggaaa catggctgaa
    11281 gacggacttt tgcctaaagc atggggaaaa ctcaacaaaa atggggcacc aacttatgct
    11341 atcgtattga cgacgttatg tacgaatgtc tttttacttt cattattatt cactgacagt
    11401 gcttataact ttgcttacac acttggtaca gcagctattt tcttcacgtg gcttttctta
    11461 ggattgtacc aaatgaaact ttcttatcag cggaaagaat gggtccaatt tttagtaggg
    11521 cttttagcaa gtgcctttca agtatgggct atgctagtcg tagcctttaa tgaagtaatg
    11581 cttgttcttg ttcttttctt gccaggcatt tatttttacc tcaaagcacg taaggagcaa
    11641 aacgagcctg tacaacatat ccacttagat aagattttac ttataggtgt gacgatggtc
    11701 ggagtgattg caatggtttt atttgccact gggatgcttc acgtatagaa gtataaaaga
    11761 agtataaaga gaggttgtga gcctgtcgtt tattcaaaaa catactagga aaggagctgt
    11821 gacacaaatg ttgtcacagc tcctttttca gaataaacgg tgtttaagag acagccaatc
    11881 ggcaataagg ctgaacgtct ctttcacagc ctcttttttg atgagtcatc atacattttt
    11941 tcgcttaaat acgagatatc ctaatgctaa gaacaaaacg atgtaagcta aattaccgaa
    12001 tagcatttga ttagtagaaa gatgagtcaa ttgcttcatg atctcttcgt ttccaatttg
    12061 gttttgcaga ttcaacatac tgatcggatt ccatttgatc cactcccatt tttggatgag
    12121 tgcaataagg ataccagata tgattgacga agcaaaataa aagacgattc ctgctgagat
    12181 ggatgcgccg gaactattga taaagcaagc aatcatcaac actaaactta aaatcagcca
    12241 taaaccgata taactaccca aagtataagt gaacaagctt tggatcagtg tttgaccagt
    12301 agaaaccttc tccgtgaagg aaatcgaagg gaaaagaatc agtttcatca acacagtgac
    12361 aataatagct aaaaggtata aataaacaga ataaataact aatgtgatcc atttgcttac
    12421 taatatttct cctcgagaat actttcgata aaggagattt ttcaatgtac catattgtgc
    12481 ttccatcgaa atgatcgtgc ttgcagctgc aatcatgata aagacaatcc aagatgtggc
    12541 actaaaaagc tgagcagtca tttcaactgg atcaatgatc gtcgtgtatt ttttggctag
    12601 aagtgctgtt cctatgagca aaacaactaa tagaacagat aaaacagcag ttgacttttt
    12661 cttgatcaat ttaaataatt cttgttttac aagcaatgtc atttttcttc ttcccccttt
    12721 actgaatcca gaatttccaa tagagaagat tccaaatcat tcgtcagatg ctggacgtct
    12781 gtcacatgga tattttgtgc agacaatagc tggatcacgg ttcctaataa ttcttcgttt
    12841 tcttttaaaa gcttgatttt atcaggatct tccaaagtat atccagcttc tgtcaagatc
    12901 gttcgagcag ctagatcatt tgttgtagag ataacgatga aatcttgcgc tgtttggttc
    12961 aattcagcca ttgtacattg tttgatgatc tttccttgat tgatcaggat cagctcatcg
    13021 accatttttt caagctcgct gagtaagtga ctggagatca agaaagaggt gccctgagta
    13081 gccaagtcgg taatgatttt tcgcacatct cttgttgctt gtggatccaa accgttcatc
    13141 ggctcatcta aaataacgat agacggatgg ttcagtaagg caagagcaat tcccaatttt
    13201 tgtttcattc ccaacgagta ggatttcgct tttttgtgaa tgtacttttc catcttgagc
    13261 tgattcacga tagtgtcgat agcagataca tcgttggttt cagagaataa cttcagatga
    13321 tcatagcctg ttaagaatgg atagataccc gggtattcga tcaatgctcc tacttgttcc
    13381 aaccctttat gactagaggg agaaatctca ttttcaccaa tacagattgt tccatctgag
    13441 taatggatca gtcctaaaat agctttcatg atcgttgttt ttccagcgcc attaggacct
    13501 actaatccaa cgattttgcc aggggataag ctaaaactga catcgctgag ggcttgtttg
    13561 tgaccaaagt tcttggataa ttcttggacc gaaagacttt tcaaaaatat tcactccttt
    13621 tctatagata gtacaagcat acacctgatg agtggagaag tcgctaggtc attggactta
    13681 tttcttattt tttcataaaa aaattgaata ttaaataaaa aggcttattt tttctgatca
    13741 gtacttattc caagtgtcta gtggccgcta gtaatcttga tgccaatgat actgatgagt
    13801 agcatcccca caaagaacca agtggaaaga ttcagcttat catgaaacaa gaccacacca
    13861 attatgaccg aaccaactgc gccaatccct gtccagatag gataagagag actcatcggc
    13921 aaacttttga ttgcctttga caaaaagtaa aaactagcaa tcatgccaat cactgtgtaa
    13981 aaagaataat tgattttaga
//
LOCUS       DARBDV010000262        11970 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.guided.086, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000262 DARBDV010000000
VERSION     DARBDV010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.guided.086"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1979
                     /locus_tag="SCM56_003013"
     CDS             <1..1979
                     /locus_tag="SCM56_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG1111714.1"
                     /translation="EIERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVP
                     FDDYDYHLPKRYEDKKVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSI
                     VGELDFPNSEIFYYQQQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWES
                     DNETSTNVVDALESVDELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYD
                     DLSIKLVRPFGDMLGLHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIY
                     VGYNLDTGRNIYLQPSLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGG
                     QATIVDPKAERGEWKETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAID
                     ILTFLTGISSRDSEKFPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIES
                     FVDYDFAHLLFSDGSVTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLI
                     VISTFALDFIHSDRSIFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQN
                     ADDLLDEKMKNNIGLKFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVS
                     DIYGRVGVMQFHPIFEELLHAFDTRPPVRNEVEE"
     gene            1976..4003
                     /locus_tag="SCM56_003014"
     CDS             1976..4003
                     /locus_tag="SCM56_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111715.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            4000..5022
                     /locus_tag="SCM56_003015"
     CDS             4000..5022
                     /locus_tag="SCM56_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEG1111716.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            5039..5968
                     /locus_tag="SCM56_003016"
     CDS             5039..5968
                     /locus_tag="SCM56_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1111717.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            6213..6329
                     /locus_tag="SCM56_003017"
     CDS             6213..6329
                     /locus_tag="SCM56_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEG1111718.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            6345..7148
                     /locus_tag="SCM56_003018"
     CDS             6345..7148
                     /locus_tag="SCM56_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEG1111719.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            7383..7619
                     /locus_tag="SCM56_003019"
     CDS             7383..7619
                     /locus_tag="SCM56_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111720.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            7626..9578
                     /locus_tag="SCM56_003020"
     CDS             7626..9578
                     /locus_tag="SCM56_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HEG1111721.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(9650..10147)
                     /gene="dfrG"
                     /locus_tag="SCM56_003021"
     CDS             complement(9650..10147)
                     /gene="dfrG"
                     /locus_tag="SCM56_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HEG1111722.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            10509..11549
                     /locus_tag="SCM56_003022"
     CDS             10509..11549
                     /locus_tag="SCM56_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEG1111723.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            11647..11835
                     /locus_tag="SCM56_003023"
     CDS             11647..11835
                     /locus_tag="SCM56_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEG1111724.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(11893..>11970)
                     /locus_tag="SCM56_003024"
     CDS             complement(11893..>11970)
                     /locus_tag="SCM56_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEG1111725.1"
                     /translation="SFTDKELSLMEALAKGINEARNIEG"
BASE COUNT         4051 a         2021 c         2313 g         3585 t
ORIGIN      
        1 cggaaatcga acgcttttct aagatggaac agttgttaga aagtaaatta tccagacgct
       61 ttcagattcg taaattggag aaagatgatt ttggttatat tattgaacat ctttacgggc
      121 aaacaggtgt cccttttgat gactacgact accatttacc gaaacgatac gaagataaaa
      181 aagtgttggt aaagaaatac gatattctca aacccactcg ctgtttaatc gaagaaaatc
      241 aacgctattt acggattgaa caggaagata ccactactta tgtggcgtat tttacaatta
      301 atagtattgt tggtgaattg gatttcccta atagtgaaat tttttattac cagcaacagc
      361 agtttacttt ccccgttgat accagtatga atgttgaaat tgtcgccaat aaaaaagcct
      421 tatctaccgt tcgcaataaa aagaaagaac tgaaagattt agatagccac gcatgggaaa
      481 gcgacaacga aaccagtact aatgtggtgg acgctttaga atctgtcgat gaattagaaa
      541 gtaatttaga ccaaaccaaa gaatctatgt acaaactaag ctatgtggta cgggtatcag
      601 caaagaattt ggacgagctg aaacaacgtt gtgatgaagt aaaagatttt tatgatgatc
      661 tgtctatcaa attagtacga ccttttggcg atatgctagg cttgcataat gaatttattc
      721 cctcaagcaa gcgatatatc aatgactata ttcaatatgt cacgtcggat tttttggctg
      781 gtcttggttt cggtgctaca caaatgattg gtgaaaattc tggtatttac gtcggttaca
      841 atctcgatac aggcagaaat atttatctcc aacctagcct tgcaagtcaa ggaattaaag
      901 gctctgtaac aaatgcatta gcgtccgctt ttattggttc attaggtggc ggaaaatcat
      961 tctctaacaa tatgctggtc tattattcag tactctttgg cggacaggca accattgttg
     1021 acccaaaagc tgaacgtggt gaatggaaag aaaccttacc tgatattgcc catgaaatca
     1081 atattgttaa tctcacgagt gagaacgaca ataaagggtt acttgaccct tatgtgattt
     1141 taaaactccc gaaagattca gaaagccttg ccattgatat tctcacgttc ttaacaggta
     1201 tttccagccg agatagcgag aagtttcctg tcctgcgtaa agctattcgg agtgtaactc
     1261 aaagcaaaca acgagggttg ttattggtga ttgatgaatt gcgaaaagaa gatacgccca
     1321 tcagcaatag tattgctgac catatcgaaa gctttgtcga ttatgatttt gcccatctgt
     1381 tattcagtga tggcagtgtc acccaatcca ttagcttgga aaaacaactc aatattattc
     1441 aagtggcaga tttggtctta cccgattcca atagctcttt tgaagaatac actaccatgg
     1501 aattattaag tgtggcaatg ctgattgtca ttagtacctt tgctttagac tttatccact
     1561 ctgaccgttc catttttaaa attgttgacc ttgatgaggc ttggagtttt ttacaagtgg
     1621 cacaaggaaa agctctatct atgcgattgg ttcgagcagg acgtgccatg aatgcagggg
     1681 tctatttcgt tactcaaaac gcggacgatt tactagatga aaagatgaag aataatattg
     1741 gcttgaaatt tgcctttcgt tccactgacc tagtggaaat caaaaagacc ttagaatttt
     1801 ttggtgtgga caaggaagat gaaaacaatc agaaacgctt acgtgaatta gaaaatggac
     1861 aatgtttagt ttcagacatc tatggtcgtg taggggttat gcagtttcac ccgatttttg
     1921 aagagttgct tcatgccttt gataccagac cgcctgtgcg aaatgaggtg gaagaatgac
     1981 acaaaaacaa aagaaatttc ttcactatag ttggataacg ctcctttgtg tgggcgttat
     2041 tttactttta ttaggcacgc tgggcaatgc agtacaagcg acaggcttag ttgatgaaac
     2101 cattgataca agcaatgaat actccaagta tggtttgaat caatatcagc ttgattatta
     2161 tgtggacaat agctggggct ggcttccatg gaattggagt gatggcatag gacaatcggt
     2221 gatgtatgga ttgtatgcca ttactaattt tatttggaca atttctcttt atctatcgaa
     2281 cgccacaggt tatttggtga aagaagccta tgccttagat ttcattagtc aaacatccca
     2341 agcaattggc gaaaacatgc agacgattgc aggcattaca ccgaatggct tttccagttc
     2401 agggttttat gtcggtttcc tccttctttt tattctcatt gttggaatct atgttgccta
     2461 tacaggattg gttaagcgag aaacgacgaa agtgatacga gctgttttaa actttgtcgt
     2521 ggtgtttatt ctatcggctt cctttattgc gtatgcaccc gattatattg cgaaaatcaa
     2581 tgatttttcc aaagatgtca gtaccgccag tctgaatatt gggacaaaaa ttgttcttcc
     2641 taacacggat agtcaaggga aagacagtgt ggacatgata cgtgacagct tattctctgt
     2701 tcaagtaaag caaccatggc tattgcttca atatggctcc actgatgtag aagcgttagg
     2761 ggaagaacgt gtggaaagtt tattgtctac cagtccagat acaaacaatg gtgaagatag
     2821 agaaaatgta gtgattgaag aaattgaaga tcatgacaat acctatttaa ccctaccgaa
     2881 gacgatttcc agattaggaa cagtcttctt tttgtttgta ttcaatattg gtatctcgat
     2941 ttttgttttc cttctaacag gaatcatgat tttttcgcaa gtgctattta ttattttcgc
     3001 tatgtttttg ccaatcagtt tcctattaag tatgttgccg agttttgatg gcatgggcag
     3061 gcatgccatt accaagttat ttaatgtgat tatgacaagg gctggtatta ccttgattat
     3121 taccgttgcc tttagtattt ccactatgct ttattcgctg tcagcaagtt ctcccttttt
     3181 catgattatg tttttacaaa ttgtgacctt tgcagggatt tattttaaac tgggcgattt
     3241 aatgagccta ttcactttac aaagcaacga ctctcaaaac atgggcagac aagtgtttcg
     3301 cagacctcgt caaatgatga atcgacaaac cagaagattt caacgcagta tgagtaagat
     3361 ttttcgtgga aaagcagggt caacgaagca aaagacaatg acaacaaagc agaatcaaac
     3421 caataaagct aaccatactc gtcaaaatga acggactaca ccaaagaaag aaacagcact
     3481 ttccaatgtg aaaaagcaac aaggcggtat cgggcaacga ttaggtgaaa agacagccca
     3541 tgtattggac acgaaaaatc gtctagtcaa taaaagaaag caggcaaaac aacagataca
     3601 ggcgacacca acccatgtca aatatgccct tcatcaagga aaagagaatg tcactcataa
     3661 tgtaacagac tttaaacaat ctatggcaga taccaaagct caacgcaaac aacagcgtat
     3721 gaaccagcag gagcaaaaac gaaagaacat tgctcaaaag cgtttggagc tggataaagc
     3781 aaaagaaaaa agacgaaaag aaaacccatc atctcctgtt cagaataacc agcagcatgt
     3841 tcgcaagcgt cagcaaaccg tcacgcctgt tttgtcacaa aacactaaac gtgaaaatca
     3901 tcagcgacca ttgaaaaaag agcaaaaaca aaagacacca ccgaagcaat tcaatcagag
     3961 gaagctaaat aaaagaacgg ataagcgagg acaacaaaca tgaagctaaa aataacagtg
     4021 atttgtagtg tggtcttctc tctcttcttt tttctcttat tattcctagt cattttcttt
     4081 gcagatgata cggacagtgg cgaaaacaac aaggattctt ctattccaca aggcggtgtg
     4141 accgtttcac cagaagtgtt ggcacaccgt ccgttgattg aaaaatatgg taaggaatat
     4201 gggattgaag actatgtttc ttatatctta gccattatgc aagtggaatc gggcggtaca
     4261 gcagaagatg ttatgcaaag ttccgaatct ttgggtttac cgcccaacag tttgagtacc
     4321 gaagagtcca tcaaacaagg ggttaaatat ttcagtgaat tattaaccag tgcagaacaa
     4381 caaggagtgg atatagattc tgttatccaa tcttataatt atggcggtgg ctttttaaat
     4441 tatgtgagga gtcatgggaa aaaatatacc tatgaattag ccgaacagtt ttctaaagag
     4501 aagtcgggtg gtcaaaaagc agactatccc aatcctatag ccatacctgt gaatggtggt
     4561 tggcgatata actatggaaa tcagttttat gttcagttgg tttcacaata cctaaccgac
     4621 acttccccaa cagaatttga tgatgaaacc gtgcaagtca ttatggacga agccttgaaa
     4681 tacgaagggt ttccctatgt ctttggaggg gcgtcgccta ccacctcttt tgattgtagt
     4741 ggcttaatac aatgggtgta tgacaaagct ggcatttctc tccctcgtgt cgcccaagat
     4801 cagtatgacg ctacgcaaga aatctctatg gaagaagccc aagcaggcga ccttatcttc
     4861 tttcattcaa cctataatgc aggcacatat gtgactcatg tggcgatata tttagaggga
     4921 aatcgctttt atcatgcagg cgacccaatc ggttatggcg acttaagcag tcgttattgg
     4981 caagaccatc tgattggggc tagacgtgtt attcataact aagaaaggaa tgaatcaaat
     5041 gattataaaa atcgagcgaa aagaaaagaa ggagaaacca aagaaatcta aaaaaccgag
     5101 caagcaaaga aaagttccga tgattaaagt tggcacgcat aagaaattaa cctttgtttt
     5161 atgggtttta ttgattggta gtgtcggatt tggaatctat aaaaatttta ctgccattga
     5221 cactcatact gtccgtgaaa cagaaatcat taaacagcag attgtcgata ccaaccaagt
     5281 agaaagtttt gtgaaatcct ttgcgaaaga ctacttttcg tggcaacaat cccaagaagc
     5341 cattgataaa cgaaatgaaa aattgaccca ttatttgacg gaagaattgc aggtattaaa
     5401 tgaagaaatg attcgtaagg atattcctac tagttctagt gtgaacgata tacaggtgtg
     5461 gcaggtttct caagtgaatg agaacacctt tgaggtactg ttttctgttg aacaagttat
     5521 taccgaagac aaagacaagg aaactatatc gtctagcttt catgtggttg tccatataga
     5581 tgaatcagat aatatggtta ttatcaaaaa tccgacaatg agtaagaaac ctcaaaaatc
     5641 ggactaccaa cccaaacagc tagaaagtga ccatacagta gatacagaaa cgatggacga
     5701 aatcactagc tttttagaaa cgttcttcca gctctatcct accgccacag aaaaagaatt
     5761 gacttactat gtaagtaatc atgtgttacc tatgattaac aaggaatatg ttttcgagga
     5821 attggtgaat cctatcttta ccagaaaaga taaccaagta atcgtgaatg tcgctgtgaa
     5881 atatttagat caagaaacaa aggcaaccca aatctcgcaa tttgagttga tattagaaaa
     5941 acaagataat tggaagattg taaaataaca aatattggta catgattaca gatactttgt
     6001 aatcatgtac tctttttgat aaaaaattgg agattccttt acaaatatgc tcttacgtgc
     6061 tattatttaa gtgactattt aaaaggagtt aataaatatg cggcaaggta ttcttaaata
     6121 aactgtcaat ttgatagcgg gaacaaataa ttagatgtcc ttttttagga gggcttagtt
     6181 ttttgtaccc agtttaagaa tacctttatc atgtgattct aaagtatcca gagaatatct
     6241 gtatgctttg tatacctatg gttatgcata aaaatcccag tgataaaagt atttatcact
     6301 gggattttta tgcccttttg ggtttttgaa tggaggaaaa tcacatgaaa attattaata
     6361 ttggagtttt agctcatgtt gatgcaggaa aaactacctt aacagaaagc ttattatata
     6421 acagtggagc gattacagaa ttaggaagcg tggacaaagg tacaacgagg acggataata
     6481 cgcttttaga acgtcagaga ggaattacaa ttcagacagg aataacctct tttcagtggg
     6541 aaaatacgaa ggtgaacatc atagacacgc caggacatat ggatttctta gcagaagtat
     6601 atcgttcatt atcagtttta gatggggcaa ttctactgat ttctgcaaaa gatggcgtac
     6661 aagcacaaac tcgtatatta tttcatgcac ttaggaaaat ggggattccc acaatctttt
     6721 ttatcaataa gattgaccaa aatggaattg atttatcaac ggtttatcag gatattaaag
     6781 agaaactttc tgccgaaatt gtaatcaaac agaaggtaga actgtatcct aatatgtgtg
     6841 tgacgaactt taccgaatct gaacaatggg atacggtaat agagggaaac gatgaccttt
     6901 tagagaaata tatgtccggt aaatcattag aagcattgga actcgaacaa gaggaaagca
     6961 taagatttca gaattgttct ctgttccctc tttatcatgg aagtgcaaaa agtaatatag
     7021 ggattgataa ccttatagaa gttattacta ataaatttta ttcatcaaca catcgaggtc
     7081 cgtctgaact ttgcggaaat gttttcaaaa ttgaatatac aaaaaaaaga caacgtcttg
     7141 catattaagt agttcagttt tggagtacaa aaagtgaaaa tgtaaaccta aatgagtatc
     7201 aaattgcaac tgaatttgat actcgttttt tattgttaaa tcaacgtttg ttgttaattt
     7261 acaccacaaa atgtaagtta aaatgtatat aaaaatgcga acgtgaaata attattgtag
     7321 gaatgcaaat tggtgtgtta aaatggttac aatttactaa gcaaaaaggg gtgcattttc
     7381 ctatgcaatt tcttgagcca aaaaataaga acgctaagtc tgtggattgg gaaatatcag
     7441 agcaagtaag ggtgatagtt aagcaatacg ctgaatatgc agagcgtact gaaagcgaag
     7501 cagtggatga attcttattg aacattttag acgataagaa gtttattgaa tggattgcca
     7561 acaagcgaag taataagcgg attgttgaaa aaatgggtat caaagataga gtgggttgat
     7621 agacaatgac caagcttaaa agattagcca cgaaggaaga tgaaattgta gatgttaaaa
     7681 ttccgatttc aaacgaagaa ttaaaagata gggctaaaca atatgattta ttaactccaa
     7741 aacgatttgc aacaaggtat aacaaaatgt tgttcttgcc aacgtctttt aaatggaacg
     7801 gttctgaata tcccattcaa tataattact gcatcaatcc gttttgttgt aattttggga
     7861 aagaacagca caaattcaaa gatgtaaaag ggaaaccaag tcgatataaa atgactggta
     7921 gttccaaaga taaaggtcat aaaggtatgt actgcaatga caatcctatt ggcagaggtg
     7981 tatcgcaaaa ctgtacagta acccctttat ctaattggtc tgttgttgaa gaaattaaac
     8041 gccttataga aatcaatagc atccaagatg ttgagccaga ttatcaattt cataaagaag
     8101 gctgctcgga agaagaatca accccattca atgagccgaa gcaattttac aaaaggggga
     8161 aaagtagggg caaatctcaa cgctatcaat gtaaggcgtg taagaagttc actaatgttc
     8221 taccaaaaag agaagaaacc actacttatc accaacaaaa gaacaccatt cttccaatgt
     8281 tcgctaagat ggttgttgga agggtttcag tcagtcgtac ttgtgatatt ttaggaattg
     8341 gagttggaac ctactatcat aagctggagt ggctgtatag gcgttgtttg gagtttttag
     8401 aacgatatga gacacaaccc cttcaaacaa agaaatttaa cgagatgtgg cttaatacgg
     8461 acaagatgca ctactaccta aataacgtcc gtaagaaagg acaaggttca aagaaatata
     8521 cggggtttga agaccttaat atgcaaacgt atatagttgt atctgctgag gtcttatcaa
     8581 gatatgtgtt ccgttctgat gttgcctatg attggaatat ttcaatggat gaattaaatg
     8641 aagatacaag aaaattcaaa gaagaccatc tcaatacttt tagtcgtaaa aatgataggt
     8701 tagattggtc ttattatcca caagaaccat ccgctaacga tagcgaaaat aggaatgctt
     8761 atcttcacga attagggaaa attactaata ggagtagatt tgtcgatggt ttaaatgttg
     8821 atgctcctta tactacaact gctcactatt ggttaattaa acaaatggtt aatgccgacg
     8881 aatggcgaat gattagtgat gatgacttct ctattagaaa cgccttttat agagtattca
     8941 cgaaagaatt aaggttgagc gatgcacatc attttatttg ccaagttaac aagacgaaaa
     9001 gcagaaaaca atgcttaaaa gaattcggac aagcaaaagc agaactgctc gattggggag
     9061 atatcagagg attcaaaaca aagtttctac ggactttggc atctcattat ctaactgaat
     9121 tactaacaag tcatcaattc cacgaagagg ctattagtaa agatggagaa agatatcgca
     9181 aatatgccga taaccctatc aaacatccat tagccactaa ggataaagga ttttattcag
     9241 ttgattgtag aacggattta tctgctctgg aaccaaacga aatcgcaaag atgctattaa
     9301 atgttaatga ccattcgacc aatagcttta ttcaacagat aagacgttat atatcatctc
     9361 ttgaaagacc attaacaaca gctcgtggtg ataagaagag ctatatttac gctaacttca
     9421 atccgaagta tgctcaattt gcgattacaa ttctaagaac ttactacaac ttttgtcgcc
     9481 cttttaaatc agcggataaa aaggttttga cacctgctca aagattaggg attacagata
     9541 aacaatttga ttggaaagat ataatttatt tcaaatgaca aaaagagctt ggagtaatcc
     9601 caagctcttg tgctttatct ttactgttct tcttcaatta acgcacccgt taactcaata
     9661 agttttttct ttcatataca tgaaaatagt agttatacgg atttttatca ttctttatcc
     9721 ctttttgggc aaatacctca ttccattcct cataattcac ttctggaaaa aaagtatctc
     9781 cttcgaattc atgatgtatt tttgtgatgt acattttctc aacataaggg aaaaacaaat
     9841 tataaatctg ttctcctccg aaaataaaaa tttcttcttc gtttttacat aactcaaaaa
     9901 catcttctat tgaatgaaca atttcacaac cattaaaggt aaacccctta tctctcgtca
     9961 gaataatatt tcttctgtca ggtaaggctc ttccgattga ttcaaggttc ttcctaccta
    10021 atattatcgg atgtcccttt gtagtatttt taacatattc ccagtccttg ggaatcctcc
    10081 aaggaatgtc attctctttg ccaatcactc tattcttatc catcgcagca atcaaagaaa
    10141 ctttcattta taacacagcc tttttaacta atttaccatc taatttctta tttctatcaa
    10201 aaaacctttt ctcattatcc tgcccgttag ttgaataagc aattttttct ataagttata
    10261 ttatcataat accacaaaat cacaaatttt cattcaatta tcattttcaa ttgcatttta
    10321 taaatcattt tgtatgtaat ttggtatgta agttaaaatg aaaaaagtag tccattttga
    10381 agtacatttt gtcattttgt actccaaaac tgaactactt acttgcatat atacgccttt
    10441 atagtggagt actacattta cgagattcgg ttagagtatc agaaaaagaa aaaataaaag
    10501 ttacagaaat gtatacttca ataaatggtg aattatgtaa gattgataga gcttattctg
    10561 gagaaattgt tattttgcaa aatgagtttt tgaagttaaa tagtgttctt ggagatacaa
    10621 aactattgcc acagagaaaa aagattgaaa atccgcaccc tctactacaa acaactgttg
    10681 aaccgagtaa acctgaacag agagaaatgt tgcttgatgc ccttttggaa atctcagata
    10741 gtgatccgct tctacgatat tacgtggatt ctacgacaca tgaaattata ctttctttct
    10801 tagggaaagt acaaatggaa gtgattagtg cactgttgca agaaaagtat catgtggaga
    10861 tagaactaaa agagcctaca gtcatttata tggagagacc gttaaaaaat gcagaatata
    10921 ccattcacat cgaagtgccg ccaaatcctt tctgggcttc cattggttta tctgtatcac
    10981 cgcttccgtt gggaagtgga atgcagtatg agagctcggt ttctcttgga tacttaaatc
    11041 aatcatttca aaatgcagtt atggaaggga tacgctatgg ttgcgaacaa ggattatatg
    11101 gttggaatgt gacggattgt aaaatctgtt ttaagtacgg tttatactat agccctgtta
    11161 gtactccagc agattttcgg atgcttactc ctattgtact ggagcaagcc tttagaaaag
    11221 ctggaacaga attgttagag ccatatctta gttttaaagt ttatgcacca caggaatatc
    11281 tttcacgggc atataacgat gctcccaaat attgtgcaaa tatcgtaaat actcaactga
    11341 aaaataatga ggtcattatt attggagaaa ttcctgctcg atgtattcaa gattatcgca
    11401 atgatttaac tttttttaca aatgggctta gtgtttgttt agcagagcta aaaggatatc
    11461 aggttaccac tggcgaacct gtttgccaga cccgtcgtct aaatagtcgg atagataaag
    11521 taagatatat gttcaataaa ataacttagt gcgttttatg ttgttatata aatatggttt
    11581 cttattaaat aagatgaaat attctttaat atagatttga attaaagtgg aaaggaggag
    11641 attgttatta taaactacaa gtggatattg tgtcctattt gtggaaataa aacaagacta
    11701 cgaatacgag tggatactat acttaaaaat ttccctttat actgccccaa atgtaagaac
    11761 gaaactttaa ttaatgttca aaaaatgaat ataataacaa tcaaagagcc agacgccaag
    11821 acgcagagcc gataatttga gaaatgaaac tctcatctta tcggctcttt ttgtttatct
    11881 gaattttact gactagcctt caatatttct tgcttcattg ataccttttg ctagtgcttc
    11941 cattaaggat agttctttgt ctgtaaagct
//
LOCUS       DARBDV010000263         8425 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.guided.113, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000263 DARBDV010000000
VERSION     DARBDV010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.guided.113"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..185
                     /locus_tag="SCM56_003025"
     CDS             <1..185
                     /locus_tag="SCM56_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111726.1"
                     /translation="IADWLIGMNASPLYTLNLQQKGVQGTFSLGRVQTPTLYLIYQRQ
                     EAIENFKKEPFFLNNS"
     gene            481..978
                     /locus_tag="SCM56_003026"
     CDS             481..978
                     /locus_tag="SCM56_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HEG1111727.1"
                     /translation="MLFLRDGEIKATLTTMMNKLAFSHKLILEPLFKSVSQIDEESDC
                     ERMDAIDKLMEQLLEERNTLIALMSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTN
                     SASGVLRANEIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLK
                     EKVVR"
     gene            979..1395
                     /locus_tag="SCM56_003027"
     CDS             979..1395
                     /locus_tag="SCM56_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HEG1111728.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            1415..2959
                     /locus_tag="SCM56_003028"
     CDS             1415..2959
                     /locus_tag="SCM56_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1111729.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            3102..3326
                     /locus_tag="SCM56_003029"
     CDS             3102..3326
                     /locus_tag="SCM56_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1111730.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            3341..4210
                     /locus_tag="SCM56_003030"
     CDS             3341..4210
                     /locus_tag="SCM56_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEG1111731.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            4191..4925
                     /locus_tag="SCM56_003031"
     CDS             4191..4925
                     /locus_tag="SCM56_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG1111732.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4958..5866
                     /locus_tag="SCM56_003032"
     CDS             4958..5866
                     /locus_tag="SCM56_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HEG1111733.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            5863..6405
                     /gene="sat4"
                     /locus_tag="SCM56_003033"
     CDS             5863..6405
                     /gene="sat4"
                     /locus_tag="SCM56_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HEG1111734.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            6498..7292
                     /locus_tag="SCM56_003034"
                     /note="aph(3')-IIIa"
     CDS             6498..7292
                     /locus_tag="SCM56_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HEG1111735.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            7568..8140
                     /locus_tag="SCM56_003035"
     CDS             7568..8140
                     /locus_tag="SCM56_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEG1111736.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPISLGFYEQNWYLIGFAG"
BASE COUNT         2936 a         1339 c         1852 g         2298 t
ORIGIN      
        1 aaattgccga ttggttgatc ggcatgaacg caagcccttt atataccttg aatttacaac
       61 aaaaaggcgt acaaggtaca ttttcattag gacgtgtaca aacaccgact ttatatctca
      121 tttatcaacg ccaggaagca atagaaaact ttaaaaaaga accttttttc ctgaataatt
      181 catagtttta ctaatagtct ttcctaacat tgatttaata gtctttaaag tattctgctt
      241 catcacccat taggtgatga aaaaagaacc cctttctgat ttagcggaca aatactttga
      301 cctaatctca gaagatataa aagaaaaaat aataaaatat ccgatttata ctttgtataa
      361 agaactaaag tagttagcaa atcatttaca gcaaatcact atgttccaca taccatttta
      421 catacttacc ttatccacga cacatgttga gatagtatcg aagataaaga aagttgctcc
      481 atgttgtttt tgcgagatgg ggaaatcaaa gccacattga caaccatgat gaataagctt
      541 gctttcagtc ataagctaat cctagaacca cttttcaagt cagttagcca aatcgatgaa
      601 gaaagcgact gtgaaagaat ggacgctatt gataagctaa tggagcaact attggaagaa
      661 cgcaataccc ttattgcact gatgtccaaa ggtttcctag aacctgctct ttttaatcag
      721 gaacgaaatg ttttagatag tgagattaaa aatcttacta ccgaaaaaac aaacctggta
      781 acgaactctg cgagtggggt tttgcgagca aacgagataa aggacctcat taattatgtg
      841 tcagcagata attttaatgg tgactacacg gaagaactat ttgaagaatt tgtagtgaac
      901 atcattgtaa attccaggga tgagctgaca ttcaatttga aatgcggtct ttccctgaaa
      961 gaaaaggtgg tgagataaat ggcatatatt ccatatggat acaaaattca agatggagtg
     1021 gttactgtcg atgaaaaggc agcaggtcaa gtaaaggtat tctttgagaa atacatatca
     1081 ggactatccc ttacagtggc tggcgaacag gcaggtattg aaaagacaca ttctgtgatg
     1141 gggcgcattt tgaaaaacgt caactacctt ggaaatgata cgtatccagc aattattgat
     1201 aaagagatat ttgataaagc tgaagaagtt agagataaac gtgcaaagga tttaggacga
     1261 gtggtagagc ttgccgcttt cacctctccc cctcccaaag aacgattcaa aatgaaaaag
     1321 gcagataata agatgccagt ggatcctttt gaacgagcag aatacttata cagtctgata
     1381 gaaagcgagg aataaagtga cagagaaaaa tataatggtt attcctgctc gtaaaagagt
     1441 aggaagtaca gccgcaaaag aaaagataaa gaaacttcgt gttgctgcct attgccgtgt
     1501 ttctacagaa acagaagaac aaaattctag ctatgaggtt caggtctcac actatacaga
     1561 gtttataaag aaaaataatg aatgggagtt tgctggcatc tttgcagatg atggtatctc
     1621 tggtacgaac actaaaaagc gtgacgaatt taatcgcatg attgcagagt gtatggacgg
     1681 taacatcgac atggttatta caaaatccat cagccgattt gcgcgtaaca ccctagactg
     1741 ccttcaatac attagacagc tcaaggataa gaacatatca gtttattttg agaaagagaa
     1801 catcaacacc acggatgcca aaggtgaggt tttgctgact attatggcat ctttggcaca
     1861 gcaggaaagc cagagccttt cacaaaacgt taagcttggg ctacagtacc gataccaaca
     1921 aggaaaggtg caggtcaacc ataagcgatt tatgggctac acgaaagatg aagatggaaa
     1981 tttaactatt gtccccgaag aagctgagat tatcaaacgc atctaccgag aataccttga
     2041 gggtcagagt ctagtgggca ttggtcgagc ccttgaaaag gatggtattt taacagcagc
     2101 gggaaaacca agatggcgac cagaatcagt taagaagata cttcaaaacg aaaaatacat
     2161 cggagatgcc cttctgcaaa agactgtcac agtagatttt ctgaccaaga aacgagtgaa
     2221 aaatgaaggg catgttcccc aatattatgt tgaaaatagc catgaagcga ttattcctaa
     2281 agagttattt ttgcaggcgc aagaagaaat tcatcggaga agcaatatct acacaggaga
     2341 aggcaagaac aaaagaattt acagtagcaa gtacgcttta agtgccatca ccttctgtgg
     2401 agattgtggc gatatttata gacgggtcta ttggaatatc cacggaagaa aagaatttgt
     2461 ctggcgatgc gtgactagaa tcgagcaagg tcctgaagtt tgtaagaacc gaaccgtaaa
     2521 agaagatgaa ctttatggtg cagtaatgac tgcgattaat aagctacttg caggtgggaa
     2581 caatatgata aagacactgg aagagaatat tcatgctgtg attggcgaaa ccacagaata
     2641 ccaaatttca gagattaaca actcactaga ggaaaaacaa aaagaactaa tcaagctggc
     2701 gaacaagggt caagactatg accacttagc agatgagatt gatgagctga gagacaagcg
     2761 acagatcctt ttagtagaag atgcctccct cagtggcgag aacgagcgaa tcaatgagct
     2821 gattgaattt atccgcaaga acaaattccg caccttagaa tatgatgata agcttgtaag
     2881 gaagataatc cagagcgtta cagtctatga aggccacttc gtcatagcct tcaaatctgg
     2941 catcgaaatg gaaatatgaa aaccagaagt aaatagccca tgactctaca gtagagttgt
     3001 gggttttctt ttgcttgctg attataataa tattttaata actattgtgt ttatcaacca
     3061 aatggtttat aatattctta gcgtaaactt ggaggtgctg tatgactacg gcagaaatga
     3121 ttaaagaact gtgtgagcaa atgaatataa gtgtttccga acttgctaga cgtattggcc
     3181 agactccaca gaatttcaat aaaaaattac aacgagaaac agtaaccttg gatgagttga
     3241 aggccattgc tgatgtgcta ggtgtcaagt ttgtgcaggc atttatttta ccagatggag
     3301 aagaaataaa aatatctaac gaataaagga ggatgtttat atggtaggta atgttattca
     3361 aatagtaaca gagaaattat catctttgcc ttttatagaa ggcatcgtat tagggggctc
     3421 acgtgcaaga ggcacccata cagaaaattc tgatatagat atcggaatct attacaattc
     3481 agactcattt gacctgacag cgattaatca aattgctaca gagttggatg atgagaatag
     3541 aaacaacctt gttgtacctc ccggagcgtg gggtgattgg gttaatggcg gtggatggtt
     3601 agttataaac gggtgtcatg ttgacttgat attacgtgat ataaaacggg tggaacaaat
     3661 aatcaaagat acggagcaag gaattgttac tgccaattat cagaccgggc atccccatgg
     3721 ttatataagt gctatgtatc gtggagaatt agcgattagt aaaatactat atgctaagaa
     3781 cgaaagctta tgcgaattaa aaaagcaggc agaaatttac cctactgctc taaagaaaag
     3841 cttgatgaac ttttttatat ttgaagctga attctcttta atgtttgtaa aagcaaatgc
     3901 gggggcagag gataaatatt atattgcagg ccatgttttt cgtattattt catgcttaaa
     3961 tcaagtacta tttgcatgta ataatgctta ttgcattaac gaaaagaaag ctataaaact
     4021 gcttgaaact tttgaatata aacctgaaaa atatgccgaa agagttaatc atatttttga
     4081 agtactcggt ctttctcttt ttgaatgcta cgacatgacc gagaaacttt ataaagaagt
     4141 gaatgaaatt gtatcggaga taaataactt tttaaacgag gagagttcag atgaaagaaa
     4201 acaaatatga tgataatata ttttttcaaa aatacagtca aatgagtcgc tcgcagaaag
     4261 gactggctgg tgcgggagaa tgggagactt tgaaaaagat gctacctgat tttaagggta
     4321 agcgtgtgct tgatttagga tgcggctatg gatggcactg tatatatgcg atggaaaacg
     4381 gtgcttcctc tgtagtaggt gttgatattt ctcataaaat gctcgaagta gcaaaaggaa
     4441 aaacccattt tccacagatt gaatatgaat gctgtgccat agaagatgtg gatttcccag
     4501 aggagagctt tgatgtaata ctaagttcgc ttgcgtttca ttatgtagca gactatgaga
     4561 atttaataaa aaagatatat aggatgctga aggctggtgg caatttagtt tttacagttg
     4621 aacatcctgt ttttactgct catggaacac aagactggta ttataacgaa aaaggagaaa
     4681 tactgcattt cccggtggac aattattatt atgagggcaa acggacagct atgtttttgg
     4741 aagaaaaggt tacaaaatat catagaacac tgaccacata tctaaataca ctgctttcaa
     4801 atagttttat aataaatcag attgtggagc cacagccgcc agagaacatg atggatattc
     4861 cggggatggc ggatgaaatg cgacgcccaa tgatgctgat tgtatcggca aaaaagaaga
     4921 tgtaataata tagaaaaaat aaacgaggag tatgtaaatg agatcagaaa aagaaatgat
     4981 ggatttagta ctttctttag cagaacagga tgaacgtatt cgaattgtga cccttgaggg
     5041 gtcacgcgca aatattaata tacctaaaga tgaatttcag gattatgata ttacatattt
     5101 tgtaagtgat atagaaccgt ttatatctaa tgatgactgg cttaatcaat ttgggaatat
     5161 aataatgatg caaaagccgg aggatatgga attattccca cctgaagaaa agggattttc
     5221 ctatcttatg ctatttgatg attacaataa aattgatctt accttattgc ccttggaaga
     5281 gttagataat tacctaaagg gcgataaatt aataaaggtt ctaattgata aagattgtag
     5341 aattaaaagg gacatagttc cgactgatat agattatcat gtaagaaagc caagcgcaag
     5401 ggagtatgat gattgctgca atgaattttg gaatgtaaca ccttatgtta ttaaaggatt
     5461 gtgccgtaag gaaattttat ttgctattga tcattttaat cagattgttc gccatgagct
     5521 gctgagaatg atatcatgga aggtcggcat cgaaacaggc tttaaattaa gtgtaggcaa
     5581 gaactataag tttattgaaa ggtatatatc cgaggatttg tgggagaaac ttttgtccac
     5641 ctaccggatg gattcctatg aaaacatatg ggaagcatta tttctatgcc atcaattgtt
     5701 cagggcggta tccggtgagg tggcggaaag gcttcattat gcctatccgg agtatgatag
     5761 gaatataaca aaatatacca gggacatgta taaaaaatac actggtaaaa ccggctgcct
     5821 ggatagcaca tatgccgctg atatagaaga gaggcgggaa cagtgattac agaaatgaaa
     5881 gcagggcacc tgaaagatat cgataaaccc agcgaaccat ttgaggtgat aggtaagatt
     5941 ataccgaggt atgaaaacga gaattggacc tttacagaat tactctatga agcgccatat
     6001 ttaaaaagct accaagacga agaggatgaa gaggatgagg aggcagattg ccttgaatat
     6061 attgacaata ctgataagat aatatatctt tactaccaag acgataaatg cgtcggaaaa
     6121 gttaaactgc gaaaaaattg gaaccggtac gcttatatag aagatatcgc cgtatgtaag
     6181 gatttcaggg ggcaaggcat aggcagcgcg cttatcaata tatctataga atgggcaaag
     6241 cataaaaact tgcatggact aatgcttgaa acccaggaca ataaccttat agcttgtaaa
     6301 ttctatcata attgtggttt caaaatcggc tccgtcgata ctatgttata cgccaacttt
     6361 gaaaacaact ttgaaaaagc tgttttctgg tatttaaggt tttagaatgc aaggaacagt
     6421 gaattggagt tcgtcttgtt ataattagct tcttggggta tctttaaata ctgtagaaaa
     6481 gaggaaggaa ataataaatg gctaaaatga gaatatcacc ggaattgaaa aaactgatcg
     6541 aaaaataccg ctgcgtaaaa gatacggaag gaatgtctcc tgctaaggta tataagctgg
     6601 tgggagaaaa tgaaaaccta tatttaaaaa tgacggacag ccggtataaa gggaccacct
     6661 atgatgtgga acgggaaaag gacatgatgc tatggctgga aggaaagctg cctgttccaa
     6721 aggtcctgca ctttgaacgg catgatggct ggagcaatct gctcatgagt gaggccgatg
     6781 gcgtcctttg ctcggaagag tatgaagatg aacaaagccc tgaaaagatt atcgagctgt
     6841 atgcggagtg catcaggctc tttcactcca tcgacatatc ggattgtccc tatacgaata
     6901 gcttagacag ccgcttagcc gaattggatt acttactgaa taacgatctg gccgatgtgg
     6961 attgcgaaaa ctgggaagaa gacactccat ttaaagatcc gcgcgagctg tatgattttt
     7021 taaagacgga aaagcccgaa gaggaacttg tcttttccca cggcgacctg ggagacagca
     7081 acatctttgt gaaagatggc aaagtaagtg gctttattga tcttgggaga agcggcaggg
     7141 cggacaagtg gtatgacatt gccttctgcg tccggtcgat cagggaggat atcggggaag
     7201 aacagtatgt cgagctattt tttgacttac tggggatcaa gcctgattgg gagaaaataa
     7261 aatattatat tttactggat gaattgtttt agtacctaga tttagatgtc taaaaagctt
     7321 taactacaag ctttttagac atctaatctt ttctgaagta catccgcaac tgtccatact
     7381 ctgatgtttt atatcttttc taaaagttcg ctagatagag ttctatatta gagatgtaaa
     7441 gagttttggt gaagaggttt gtgagtatgg cttttataac cagggtgcaa tgagaagcac
     7501 taacagcagc tagcttaaga acgcatagga tatacttttt atggaagtca cagaggagga
     7561 ataaaatttg aacaggatta acagagtaac ctctattctt attcagctgc aatcaaaaaa
     7621 aataattcct gccaaagaaa ttgcacagcg atttaatata agcttaagga cagtttacag
     7681 agatatccgg acacttgaag aagccggaat accaattgga tctgaggccg gaaaaggata
     7741 ttttcttgta gagggcttcc tacttccgcc ggtaatgttt acagcggcgg aagtgggtgc
     7801 attgattaca gcagggaaat ttttaaattg ccatggagat gaatcattta taaaggattt
     7861 tgattcagct atgtataaaa tcaaatctat tttgaagcat ggtgaaaaaa actatgcaca
     7921 ggagttggag aacagtatta acgtgtacag cacatctgga cagaaaaata cattggcgga
     7981 taacgttatt gcagcaattc agactgcaat ctgcaataaa agggtaatat caattcaata
     8041 tcctgcatca ggagggcaag aaccggaaag cagaatgata gagcctatat cactggggtt
     8101 ttatgaacag aactggtact taatcggttt tgcaggctaa gaaatgaata taggaatttt
     8161 cgtgtggatc ggattaaaag catttgcata gaagaagagc tctgtcaaag ccatgatggc
     8221 tctttggaac aaatattaaa acaaatgctt tcatataaaa aactatatgc cctacaatgc
     8281 cgttgtaaaa attggggaaa agggatatat tgattttata tctttctcaa aataaatata
     8341 aaagcccttt aaagagggct tttatatatt aatcacaaat cacttatcac aaatcacaag
     8401 tgattaatca caaatcactt gtgat
//
LOCUS       DARBDV010000264         6097 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.guided.127, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000264 DARBDV010000000
VERSION     DARBDV010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.guided.127"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            261..1172
                     /locus_tag="SCM56_003036"
     CDS             261..1172
                     /locus_tag="SCM56_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HEG1111737.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1325..1810
                     /gene="vanZ"
                     /locus_tag="SCM56_003037"
     CDS             1325..1810
                     /gene="vanZ"
                     /locus_tag="SCM56_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HEG1111738.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            2329..2613
                     /locus_tag="SCM56_003038"
     CDS             2329..2613
                     /locus_tag="SCM56_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1111739.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            2979..3278
                     /locus_tag="SCM56_003039"
     CDS             2979..3278
                     /locus_tag="SCM56_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111740.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     gene            3321..4811
                     /locus_tag="SCM56_003040"
     CDS             3321..4811
                     /locus_tag="SCM56_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HEG1111741.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            5159..5329
                     /locus_tag="SCM56_003041"
     CDS             5159..5329
                     /locus_tag="SCM56_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111742.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            5343..5960
                     /locus_tag="SCM56_003042"
     CDS             5343..5960
                     /locus_tag="SCM56_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1111743.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            5960..>6097
                     /locus_tag="SCM56_003043"
     CDS             5960..>6097
                     /locus_tag="SCM56_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
                     /protein_id="HEG1111744.1"
                     /translation="MSTVILAEKPSQALAYASALKQSTKKDGYFEIKDPLFTDETFIT
                     FG"
BASE COUNT         2253 a          879 c         1161 g         1804 t
ORIGIN      
        1 accttatttg atagtagatt taaaactttg caacagaacc cccattgtca acaggcagtt
       61 cagcctcgtt aaattcagca tgggtatcac ttatgaaaat tcatctacat tggtgataat
      121 agtaaatcca gtagggcgaa ataattgact gtaatttacg gggcaaaacg gcacaatctc
      181 aaacgagatt gtgccgttta aggggaagat tctagaaata tttcatactt ccaactatat
      241 agttaaggag gagactgaaa atgaagaagt tgtttttttt attgttattg ttattcttaa
      301 tatacttagg ttatgactac gttaatgaag cactgttttc tcaggaaaaa gtcgaatttc
      361 aaaattatga tcaaaatccc aaagaacatt tagaaaatag tgggacttct gaaaataccc
      421 aagagaaaac aattacagaa gaacaggttt atcaaggaaa tctgctatta atcaatagta
      481 aatatcctgt tcgccaagaa agtgtgaagt cagatatcgt gaatttatct aaacatgacg
      541 aattaataaa tggatacggg ttgcttgata gtaatattta tatgtcaaaa gaaatagcac
      601 aaaaattttc agagatggtc aatgatgctg taaagggtgg cgttagtcat tttattatta
      661 atagtggcta tcgagacttt gatgagcaaa gtgtgcttta ccaagaaatg ggggctgagt
      721 atgccttacc agcaggttat agtgagcata attcaggttt atcactagat gtaggatcaa
      781 gcttgacgaa aatggaacga gcccctgaag gaaagtggat agaagaaaat gcttggaaat
      841 acgggttcat tttacgttat ccagaggaca aaacagagtt aacaggaatt caatatgaac
      901 catggcatat tcgctatgtt ggtttaccac atagtgcgat tatgaaagaa aagaatttcg
      961 ttctcgagga atatatggat tacctaaaag aagaaaaaac catttctgtt agtgtaaatg
     1021 gggaaaaata tgagatcttt tattatcctg ttactaaaaa taccaccatt catgtgccga
     1081 ctaatcttcg ttatgagata tcaggaaaca atatagacgg tgtaattgtg acagtgtttc
     1141 ccggatcaac acatactaat tcaaggaggt aaggatggcg gaatgaaacc aacgaaatta
     1201 atgaacagca ttattgtact agcacttttg gggtaacgtt agctttttaa tttaaaaccc
     1261 acgttaacta ggacattgct atactaatga tacaacttaa acaaaagaat tagaggaaat
     1321 tatattggga aaaatattat ctagaggatt gctagcttta tatttagtga cactaatctg
     1381 gttagtgtta ttcaaattac aatacaatat tttatcagta tttaattatc atcaaagaag
     1441 tcttaacttg actccattta ctgctactgg gaatttcaga gagatgatag ataatgttat
     1501 aatctttatt ccatttggct tgcttttgaa tgtcaatttt aaagaaatcg gatttttacc
     1561 taagtttgct tttgtactgg ttttaagtct tacttttgaa ataattcaat ttatcttcgc
     1621 tattggagcg acagacataa cagatgtaat tacaaatact gttggaggct ttcttggact
     1681 gaaattatat ggtttaagca ataagcatat gaatcaaaaa aaattagaca gagttattat
     1741 ttttgtaggt atacttttgc tcgtattatt gctcgtttac cgtacccatt taagaataaa
     1801 ttacgtgtaa gatgtctaaa tcaagcaatc tgatctttca tacacataaa gatattgaat
     1861 gaattggatt agatggaaaa cgggatgtgg ggaaactcgc ccgtaggtgt gaagtgaggg
     1921 gaaaaccggt gataaagtaa aaagcttacc taacactata gtaacaaaga aagcccaatt
     1981 atcaatttta gtgctgagga attggtctct ttaataaatt tccttaacgt tgtaaatccg
     2041 cattttcctg acggtacccc cactagcagc catgcttttt ttgaatcttt gtaggacgtt
     2101 ttaaagagaa ttacccactt taaaattaaa catgcgctta gaatcgcttt aggaaacacg
     2161 atccagtcca ataatcgtcg ataaaaactt ttgaaaaagg ttggtgaaat tacctacttt
     2221 tggaataatc acaaatcaca agtgattaat cacttgttta ttaagatatt aaaagctata
     2281 atttaaataa agcgtgaatt ttattacaca aaaagagggg ggagaaactt ggaactagca
     2341 tttagagaaa gcttaaaaaa gatgagaggt accaaatcaa aagaaaaatt ctcccaagaa
     2401 ttagaaatga gtagatcaaa ttattcacga atagaatcag gaaaatcaga tccaaccata
     2461 aaaacactag aacaaattgc aaagttaact aactcaacgc tagtagtgga tttaatccca
     2521 aatgagccaa cagaaccaga accagaaaca gaatcagaac aagtaacatt ggatttagaa
     2581 atggaagaag aaaaaagcaa tgacttcgtg tgaataatgc acgaaatcgt tgcttatttt
     2641 ttttaaaagc ggtatactag atataacgaa acaacgaact gaatagaaac gaaaaaagag
     2701 ccatgacaca tttataaaat gtttgacgac attttataaa tgcatagccc gataagattg
     2761 ccaaaccaac gcttatcagt tagtcagatg aactcttccc tcgtaagaag ttatttaatt
     2821 aactttgttt gaagacggta tataaccgta ctatcattat atagggaaat cagagagttt
     2881 tcaagtatct aagctactga atttaagaat tgttaagcaa tcaatcggaa atcgtttgat
     2941 tgcttttttt gtattcattt atagaaggtg gagtttgtat gaatcatgat gaatgtaaaa
     3001 cttatataaa aaatagttta ttggagataa gaaaattagc aaatatctat acactagaaa
     3061 cgtttaagaa agagttagaa aagagaaata tctacttaga aacaaaatca gataagtatt
     3121 tttcttcgga gggggaagat tatatatata agttaataga aaataacaaa ataatttatt
     3181 cgattagtgg aaaaaaattg acttataaag gaaaaaaatc tttttcaaaa catgcaatat
     3241 tgaaacagtt gaatgaaaaa gcaaaccaag ttaattaaac aacctatttt ataggattta
     3301 taggaaagga gaacagctga atgaatatcc cttttgttgt agaaactgtg cttcatgacg
     3361 gcttgttaaa gtacaaattt aaaaatagta aaattcgctc aatcactacc aagccaggta
     3421 aaagcaaagg ggctattttt gcgtatcgct caaaatcaag catgattggc ggtcgtggtg
     3481 ttgttctgac ttccgaggaa gcgattcaag aaaatcaaga tacatttaca cattggacac
     3541 ccaacgttta tcgttatgga acgtatgcag acgaaaaccg ttcatacacg aaaggacatt
     3601 ctgaaaacaa tttaagacaa atcaatacct tctttattga ttttgatatt cacacggcaa
     3661 aagaaactat ttcagcaagc gatattttaa caaccgctat tgatttaggt tttatgccta
     3721 ctatgattat caaatctgat aaaggttatc aagcatattt tgttttagaa acgccagtct
     3781 atgtgacttc aaaatcagaa tttaaatctg tcaaagcagc caaaataatt tcgcaaaata
     3841 tccgagaata ttttggaaag tctttgccag ttgatctaac gtgtaatcat tttggtattg
     3901 ctcgcatacc aagaacggac aatgtagaat tttttgatcc taattaccgt tattctttca
     3961 aagaatggca agattggtct ttcaaacaaa cagataataa gggctttact cgttcaagtc
     4021 taacggtttt aagcggtaca gaaggcaaaa aacaagtaga tgaaccctgg tttaatctct
     4081 tattgcacga aacgaaattt tcaggagaaa agggtttaat agggcgtaat aacgtcatgt
     4141 ttaccctctc tttagcctac tttagttcag gctattcaat cgaaacgtgc gaatataata
     4201 tgtttgagtt taataatcga ttagatcaac ccttagaaga aaaagaagta atcaaaattg
     4261 ttagaagtgc ctattcagaa aactatcaag gggctaatag ggaatacatt accattcttt
     4321 gcaaagcttg ggtatcaagt gatttaacca gtaaagattt atttgtccgt caagggtggt
     4381 ttaaattcaa gaaaaaaaga agcgaacgtc aacgtgttca tttgtcagaa tggaaagaag
     4441 atttaatggc ttatattagc gaaaaaagcg atgtatacaa gccttattta gtgacgacca
     4501 aaaaagagat tagagaagtg ctaggcattc ctgaacggac attagataaa ttgctgaagg
     4561 tactgaaggc gaatcaggaa attttcttta agattaaacc aggaagaaat ggtggcattc
     4621 aacttgctag tgttaaatca ttgttgctat cgatcattaa agtaaaaaaa gaagaaaaag
     4681 aaagctatat aaaggcgctg acaaattctt ttgacttaga gcatacattc attcaagaga
     4741 ctttaaacaa gctagcagaa cgccctaaaa cggacacaca actcgatttg tttagctatg
     4801 atacaggctg aaaataaaac ccgcactatg ccattacatt tatatctatg atacgtgttt
     4861 gttttttctt tgctgtttag cgaatgatta gcagaaatat acagagtaag attttaatta
     4921 attattaggg ggagaaggag agagtagccc gaaaactttt agttggcttg gactgaacga
     4981 agtgagggaa aggctactaa aacgtcgagg ggcagtgaga gcgaagcgaa cacttgattt
     5041 tttaattttc tatcttttat aggtcattag agtataccta tttgtcctaa tatgatttta
     5101 gcagtataat tgacttggtg aataggtcat ttaagttggg cataatagga ggagtaaaat
     5161 gaaaaaattt atttatcgag ttttagaaaa tgacgaagtg gtggctattt ttaatgagca
     5221 acaatatgcg caagatttta tcgcttacga aaagacaatt tctgataagc aatttgaaat
     5281 tgaaaaagta gatattgctg attggttatt gcaaccgaga gaattttaga ggttggttga
     5341 aaatggctaa aattggttat gcacgtgtca gtagcaaaga acagaactta gatcggcaat
     5401 tacaagcgtt acagggcgtt tctaaggtct tttcagacaa attaagcggt caatcggtcg
     5461 aacgcccaca attacaagct atgcttaact atattcgtga aggggatatt gttattgtta
     5521 ctgaattaga tcgattagga cgaaataata aagaattaac agaattgatg aatcaaattc
     5581 aaattaaggg ggcaaccctg gaagtcttaa atttaccctc aatgaatggt attgaagatg
     5641 aaaatttaag gcgtttgatt aatagccttg tcattgaatt gtacaagtat caagcagaat
     5701 cagaacgaaa aaaaattaag gaacgtcagg cacaaggaat cgaaattgct aagaaaaaag
     5761 gcaaattcaa aggtcgtcag cataaattta aagaaaatga tccacgttta aagcatgctt
     5821 tcgatttgtt tttgaatggt ttatccgata aagaagttga agaacaaact ggaatcaatc
     5881 gccgaacgtt tagaaggtat cgagcaagat ataacgtgac agtcgatcaa agaaaaaaca
     5941 atgaaaagag ggatagttaa tgagtacggt tattttagct gaaaaaccaa gccaggcatt
     6001 agcctacgca agtgctttaa aacaaagcac caaaaaagac ggttattttg agatcaaaga
     6061 cccactattt acagatgaaa cgtttatcac ctttggt
//
LOCUS       DARBDV010000265         6087 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.guided.128, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000265 DARBDV010000000
VERSION     DARBDV010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.guided.128"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..2226
                     /locus_tag="SCM56_003044"
     CDS             <1..2226
                     /locus_tag="SCM56_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEG1111745.1"
                     /translation="VLGFWIVIKNTEFSIGKNRRWFGGILFYFGILLLLHAHLFGKLH
                     TGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYFLIAQPGSYLAAVLLLA
                     GGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAAKEERMKQRAEAKEARR
                     LAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQLSFVPIDSFQENIHPAN
                     LEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQDYELPTVDLLDSIPTVD
                     QSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEVQPAVGVKVSKIVNLTD
                     DIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIEAQPNHPDKLLEVPLGR
                     DVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRAKPHEVKLMMIDPKMVE
                     LNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAATGVRNISGYNEFVQQKN
                     LENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMARAAGIHMILATQRPSV
                     DVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGDMLFLPMGENKPIRVQG
                     AFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQPQDELFEEAKALVVEMQ
                     TASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKVFIEQEETSETLPDDND
                     M"
     gene            2456..3523
                     /gene="rlmN"
                     /locus_tag="SCM56_003045"
     CDS             2456..3523
                     /gene="rlmN"
                     /locus_tag="SCM56_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEG1111746.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(3716..4819)
                     /locus_tag="SCM56_003046"
     CDS             complement(3716..4819)
                     /locus_tag="SCM56_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEG1111747.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            5372..5920
                     /gene="aac(6')"
                     /locus_tag="SCM56_003047"
     CDS             5372..5920
                     /gene="aac(6')"
                     /locus_tag="SCM56_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HEG1111748.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2019 a         1052 c         1316 g         1700 t
ORIGIN      
        1 gtattaggat tttggattgt cattaagaat acagagtttt caattggtaa aaaccgacgc
       61 tggtttggcg gcatactctt ttatttcggt atcttattat tgttacatgc ccatttattt
      121 ggcaaactcc atactgggga accaaatatc atggggacga catgggattt gttagccagt
      181 gatataaaac aaagtcaagt agacaataac gttggtggtg ggatgattgg agcaatcctt
      241 tatcatttta cttacttctt gattgcacag cctggaagtt atttagcagc agtgctatta
      301 ctggcaggag gagcattcct gttcagtaat ctggaaggct atcagctgtt aaatggtatc
      361 caatcaattg gagaacgagt acaggaatta ttggaaggtg atcctgccaa gcaagctcga
      421 aagcaagcgg caaaggaaga gagaatgaaa cagcgcgcag aagcgaaaga agcaaggcgc
      481 ttagctgcaa aagaagctgc agaaaaagaa gctgcagaat atgagaagaa aaaagcggtg
      541 agccagcaaa gggaaaatac tgctgatgaa tggcaggaac cagaaaatac agagccggag
      601 cagctaagtt ttgttccaat cgacagtttt caagaaaata tccatcctgc gaatctagag
      661 aaaccagtac cagatacacc aaaacagacc aatacagcag aaggtttttc agatgagttg
      721 ccagaggatg acggtacctc attagaattt gagattgaag cagaacagga aaatcaagac
      781 tatgaattac cgacagttga tttgcttgat tctataccaa ctgtggatca aagtgatgag
      841 tataaaaaaa ttgaaaaaaa tatcggcgta ttagaacaaa ctttccaaag tttcggtgtc
      901 gatgccaagg tcgtcaaagc cagcttagga ccatccgtca caaaattcga agtccagccg
      961 gctgtagggg taaaagtaag taaaattgtg aacttaacag atgatatcgc gcttgcttta
     1021 gcggcgaaag atgtccggat ggaagcaccg attccaggta aatcactgat tggtattgaa
     1081 gtaccaaatg gaaagatcag tatggtttcc tttagagaaa tcatcgaagc acaaccaaac
     1141 cacccagata aattactaga agtaccttta ggacgtgatg tttctggacg ggtacaaacg
     1201 gctgatcttt cgaagatgcc tcacttactc gtggctggtt cgactggtag tgggaagtca
     1261 gtagcaatta atgggattat cacaagtata ttaatgcgtg ctaagcccca tgaagtaaaa
     1321 ctgatgatga tcgatccgaa aatggttgaa ttgaatatgt acaacggcat acctcatctg
     1381 ttgacgccgg tcgtgacgaa ccctagaaaa gcagcgcaag ccttacaaaa agtagtacaa
     1441 gaaatggaag aacgatatga aaaattcgcg gcaaccggag taagaaatat ttctggttac
     1501 aatgaatttg ttcaacagaa aaacttggaa aacggaacga aacatccaac cttaccattc
     1561 atcgtagtga tcgtagatga attagctgat ttgatgatgg tagccagcaa tgaagttgaa
     1621 gatgcgatta ttcgtttagc gcaaatggct cgggcagcgg ggattcatat gatcctagct
     1681 actcaacgtc caagtgtgga tgttatcaca ggaatcatca aagcaaatgt accttccaga
     1741 atggcatttg cggtatcaag cggtacagat tctcggacga tcattgattc taatggggca
     1801 gaaaagctct tagggcgagg agatatgcta ttcttgccaa tgggtgagaa taaaccgatc
     1861 cgtgtgcaag gagcattcat ttctgaccac gaagtagaac gggtcgttca atttgttaca
     1921 gatcagcaag aagctcatta tgaagagaaa atgatgccga cagatgaagt tgaaacgaca
     1981 ggtgctccag aacagccaca agatgagctg tttgaagaag caaaagcact tgtcgttgaa
     2041 atgcaaacgg caagtatttc ccttctccaa agaagatttc ggatcggtta caaccgagca
     2101 gcacgtttag tagatgaact ggaagctcat ggagttgttg gcccgtcaga aggaagcaag
     2161 cctcggaaag tattcattga gcaggaagaa acatcagaaa cgttgccgga cgataatgac
     2221 atgtaaaact tgtgtgatga agggttgaaa aacgaataac caataaaaag acaaggcatg
     2281 atgataccaa aagaaaaaat ggtttttttc catcttttct aatacggtat gatgtcttgt
     2341 ctttttagtt tttgtgagat taaagttcta ttcgatttat ttgtgaagta ttgcattaag
     2401 gaaatgaatc atgtatagta tgtagcgaaa ctttgagaaa aagagggaaa atactttgga
     2461 aaaacagtcc atttatggct tgacgaatga agaattaatc aattggttta tagaaaacgg
     2521 cgagaaaaaa ttccgagctg cccaagtttg ggaatggttg tatcaaaaac gtgtaagtaa
     2581 tttcacagaa atgacgaatc tatcgaaaca attgatcgaa aaattatcgg agcattttat
     2641 catcaatcct ctaaaacaga tggtcgtcca agaagcttcg gatggtacag taaaatattt
     2701 gtttgaactc cctgataaaa acatgattga gactgtactg atgagacaag aatatggctt
     2761 gtcagtttgt gtgacgacac aagtaggatg taatatcggc tgtacgtttt gtgcaagcgg
     2821 gctgctcaaa aagaaccgtg atctgactgc tggagaaatt gttgcacaga tcatgatggt
     2881 ccaacattat tttgatgagc gcaatcttgg cgaacgcgtt tcacatgtcg ttgtaatggg
     2941 gatcggtgag ccatttgata attacgacaa tgtaatggac tttttgcata tcatcaatga
     3001 tgccaaagga ttggcaattg gtgctcggca cattacagtt tccactagtg gattagcaca
     3061 caaaatcaag gaattcgcta ataacggctt gcaagtcaat ctggctattt ccttgcacgc
     3121 gccgaataat gaggtgcgaa catcgatcat gcggatcaac cgaagtttcc caattgagaa
     3181 attgatggaa gctgtggatg agtatttaga gaaaacaaac cgtcgaatca cgtttgaata
     3241 tatcatgttg aatcaagtaa atgatagacc agagcatgcg caacaattag cagatctatt
     3301 aaaagataag aaaaaattag catatgtcaa cttgattcca tataatccag tcagtgaaca
     3361 tgatcaatat tctcgcagtc caaaagctga tgtattgaga ttttatgatg tattgaagaa
     3421 aaacggcgta aactgtgtga tacgtaaaga acatggtaca gatattgatg cagcttgcgg
     3481 acaattgcga agcaagcaaa tgaaaaaaac agttgccaaa taatcgtatc ctactcgttt
     3541 tttcaattac cataaaagga gcagttttct taattaagga actgttcaac ttaaataaga
     3601 gaaaagattc tatagcgaaa gctataaagc tttttttgac aagaaaaacc ctcatcaagc
     3661 aaagaaatgt cctcaatttg atgacagcct gcttgatggg ggttttagca attatttaac
     3721 tgattttctt atatccttgc tggctttctt gtgtagcatg ataagtttct acgtcaagaa
     3781 tggcctgttt aaatttttct cgggtgattt cataaggcac atgtttaata tcatttgttg
     3841 ccatggcttt atctaagatt gcttctaaat cggtcgtgac attgagattc atgtcggtta
     3901 acgatactgg aagttggttc tttttcataa acacatagat tttatcaaac aaatcgaact
     3961 gctgatccat catcaataag gtcagtacac cataactgac agatgctccg tgaaggtatc
     4021 cctccatttt ttctagaaca gtcgtaccgt aatagaatga gtgtgccata gcagaattat
     4081 agttattgtt aactaaaaca gaaaccaatc cggtagtaac aatgatatcc gaaacgactt
     4141 gttcaattgc atatgaggga cgatcgtcgg cactatcttt caatgcttgc gtaccataag
     4201 tcaagagacg atcaatgcac atttttccga tttggattcc taatctatcc gtgtagccta
     4261 gatcatcgcc acgagcagaa aaagtagatt cgacttcttt ggataaagca tcgccaatac
     4321 ctgcccataa gtatttatca ggtgcttcgg caataattgc tgtatcaatg aaggtgtgta
     4381 caggtggttt tgaaggaaag aagagtccgt ccattacgcc ttcttcgtta tagatgacac
     4441 ataccgcagt gacgggcgaa cagttcgaac caatagtggg aaaggtgaag actggtttag
     4501 ctagtttttc accaatgact tttacagtat cgcaagcacg gccaccaccg actgcaaaca
     4561 ggacgtctgc tttttggaca ctttcatgct ggatcaactg ttcaatattt ccataagttg
     4621 aattaccccc gtaccagaga ctggcgacaa cctctatttc ctgtgtcaaa gctactttta
     4681 ttttgttttc tgcctttgaa agtgcagtct tccctccaat cagcactact tttgtcccat
     4741 attctttgac gattccattt gctttttcat acgcatctgt tccaatggta taatttggta
     4801 agaaaacact ttgactcata ttcatttcct tctttcttcg ttgaaacggg ttaaaatttt
     4861 aaagagtgat cgatctgact gcggctgtag atggagtaga ggtgaaccta agacaggata
     4921 agtgatgaat aaggtggcat gaacaccttg aaatcatcaa taagtatcct aaaaacgaac
     4981 aagtacaaaa acattgaaaa cgacaataat gaatttttgt tcctcttagt atttctaccg
     5041 attccctttg cttttttgta attatctaaa aattctaaaa attaagatta ttatatgaaa
     5101 acaggtttta tttgtcaatt cataattggt tttttttaaa gcaaaagata atctatatca
     5161 tgatgaacaa acagagtttt ttcctccaat tgatggcttt cctgatcttt cctattttga
     5221 ataaagtgtt tctgtataat ttttttattt tataagtaag ataaataatg aaagaataga
     5281 gatactgatt ggtagctaaa gatttttgag cgagaacatt tcgttttttc aaaaggactg
     5341 ttagaataag caagaacaag ggggaagacg tatgataatc agtgaatttg accgtaataa
     5401 tccagtattg aaagatcagc tttctgattt actgagactg acttggccgg aagaatatgg
     5461 agacagctcg gcagaagaag tagaagaaat gatgaatcca gaacgaatcg cggtagcagc
     5521 ggtagaccaa gatgagttag taggatttat tggtgcaatc cctcaatacg gtatcacagg
     5581 ttgggaattg catccattag ttgtagaaag ctcccgacga aagaaccaaa taggtactcg
     5641 attagtcaat tacttagaaa aagaagtagc ttccagagga ggaatcacga tttatttagg
     5701 tacggatgat ttagaccatg gaacaacgtt aagtcaaacg gacctgtatg agcatacatt
     5761 tgataaagtg gcttccatcc agaaccttcg tgaacatccg tatgaattct atgaaaaatt
     5821 aggttataaa atcgtaggtg tcttaccaaa tgcaaatggc tgggacaaac cggatatttg
     5881 gatggcaaaa acgattattc ctcgaccaaa ttctcaataa taaaaagagg ttgtgacaga
     5941 agtaatcagc ttcaagaaat aagaaggaat ttacgaaaat tacttcttga atttttgtga
     6001 aattccaact tatttccgaa gaagctactt ttggaacacc gtgtaccaga ggttgtgaaa
     6061 aagtcgctta gtttcgagta gtaagaa
//
LOCUS       DARBDV010000266         4804 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.guided.142, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000266 DARBDV010000000
VERSION     DARBDV010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.guided.142"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            92..787
                     /gene="vanR"
                     /locus_tag="SCM56_003048"
     CDS             92..787
                     /gene="vanR"
                     /locus_tag="SCM56_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HEG1111749.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            765..1919
                     /locus_tag="SCM56_003049"
     CDS             765..1919
                     /locus_tag="SCM56_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HEG1111750.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2134..3102
                     /locus_tag="SCM56_003050"
     CDS             2134..3102
                     /locus_tag="SCM56_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HEG1111751.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3095..4126
                     /gene="vanA"
                     /locus_tag="SCM56_003051"
     CDS             3095..4126
                     /gene="vanA"
                     /locus_tag="SCM56_003051"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HEG1111752.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4132..4740
                     /locus_tag="SCM56_003052"
     CDS             4132..4740
                     /locus_tag="SCM56_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HEG1111753.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1482 a          922 c         1119 g         1281 t
ORIGIN      
        1 catggccttt tatcttttac acaattatat ggactttatc ctataatctg ataaattatt
       61 aagggcaaac ttatgtgaaa gggtgataac tatgagcgat aaaatactta ttgtggatga
      121 tgaacatgaa attgccgatt tggttgaatt atacttaaaa aacgagaatt atacggtttt
      181 caaatactat accgccaaag aagcattgga atgtatagac aagtctgaga ttgaccttgc
      241 catattggac atcatgcttc ccggcacaag cggccttact atctgtcaaa aaataaggga
      301 caagcacacc tatccgatta tcatgctgac cgggaaagat acagaggtag ataaaattac
      361 agggttaaca atcggcgcgg atgattatat aacgaagccc tttcgcccac tggagttaat
      421 tgctcgggta aaggcccagt tgcgccgata caaaaaattc agtggagtaa aggagcagaa
      481 cgaaaatgtt atcgtccact ccggccttgt cattaatgtt aacacccatg agtgttatct
      541 gaacgagaag cagttatccc ttactcccac cgagttttca atactgcgaa tcctctgtga
      601 aaacaagggg aatgtggtta gctccgagct gctatttcat gagatatggg gcgacgaata
      661 tttcagcaag agcaacaaca ccatcaccgt gcatatccgg catttgcgcg aaaaaatgaa
      721 cgacaccatt gataatccga aatatataaa aacggtatgg ggggttggtt ataaaattga
      781 aaaataaaaa aaacgactat tccaaactag aacgaaaact ttacatgtat atcgttgcaa
      841 ttgttgtggt agcaattgta ttcgtgttgt atattcgttc aatgatccga gggaaacttg
      901 gggattggat cttaagtatt ttggaaaaca aatatgactt aaatcacctg gacgcgatga
      961 aattatatca atattccata cggaacaata tagatatctt tatttatgtg gcgattgtca
     1021 ttagtattct tattctatgt cgcgtcatgc tttcaaaatt cgcaaaatac tttgacgaga
     1081 taaataccgg cattgatgta cttattcaga acgaagataa acaaattgag ctttctgcgg
     1141 aaatggatgt tatggaacaa aagctcaaca cattaaaacg gactctggaa aagcgagagc
     1201 aggatgcaaa gctggccgaa caaagaaaaa atgacgttgt tatgtacttg gcgcacgata
     1261 ttaaaacgcc ccttacatcc attatcggtt atttgagcct gcttgacgag gctccagaca
     1321 tgccggtaga tcaaaaggca aagtatgtgc atatcacgtt ggacaaagcg tatcgactcg
     1381 aacagctaat cgacgagttt tttgagatta cacggtataa cctacaaacg ataacgctaa
     1441 caaaaacgca catagaccta tactatatgc tggtgcagat gaccgatgaa ttttatcctc
     1501 agctttccgc acatggaaaa caggcggtta ttcacgcccc cgaggatctg accgtgtccg
     1561 gcgaccctga taaactcgcg agagtcttta acaacatttt gaaaaacgcc gctgcataca
     1621 gtgaggataa cagcatcatt gacattaccg cgggcctctc cggggatgtg gtgtcaatcg
     1681 aattcaagaa cactggaagc atcccaaaag ataagctagc tgccatattt gaaaagttct
     1741 ataggctgga caatgctcgt tcttccgata cgggtggcgc gggacttgga ttggcgattg
     1801 caaaagaaat tattgttcag catggagggc agatttacgc ggaaagcaat gataactata
     1861 cgacgtttag ggtagagctt ccagcgatgc cagacttggt tgataaaagg aggtcctaag
     1921 agatgtatat aattttttag gaaaatctca aggttatctt tactttttct taggaaatta
     1981 acaatttaat attaagaaac ggctcgttct tacacggtag acttaatacc gtaagaacga
     2041 gccgttttcg ttcttcagag aaagatttga caagattacc attggcatcc ccgttttatt
     2101 tggtgccttt cacagaaagg gttggtctta attatgaata acatcggcat tactgtttat
     2161 ggatgtgagc aggatgaggc agatgcattc catgctcttt cgcctcgctt tggcgttatg
     2221 gcaacgataa ttaacgccaa cgtgtcggaa tccaacgcca aatccgcgcc tttcaatcaa
     2281 tgtatcagtg tgggacataa atcagagatt tccgcctcta ttcttcttgc gctgaagaga
     2341 gccggtgtga aatatatttc tacccgaagc atcggctgca atcatataga tacaactgct
     2401 gctaagagaa tgggcatcac tgtcgacaat gtggcgtact cgccggatag cgttgccgat
     2461 tatactatga tgctaattct tatggcagta cgcaacgtaa aatcgattgt gcgctctgtg
     2521 gaaaaacatg atttcaggtt ggacagcgac cgtggcaagg tactcagcga catgacagtt
     2581 ggtgtggtgg gaacgggcca gataggcaaa gcggttattg agcggctgcg aggatttgga
     2641 tgtaaagtgt tggcttatag tcgcagccga agtatagagg taaactatgt accgtttgat
     2701 gagttgctgc aaaatagcga tatcgttacg cttcatgtgc cgctcaatac ggatacgcac
     2761 tatattatca gccacgaaca aatacagaga atgaagcaag gagcatttct tatcaatact
     2821 gggcgcggtc cacttgtaga tacctatgag ttggttaaag cattagaaaa cgggaaactg
     2881 ggcggtgccg cattggatgt attggaagga gaggaagagt ttttctactc tgattgcacc
     2941 caaaaaccaa ttgataatca atttttactt aaacttcaaa gaatgcctaa cgtgataatc
     3001 acaccgcata cggcctatta taccgagcaa gcgttgcgtg ataccgttga aaaaaccatt
     3061 aaaaactgtt tggattttga aaggagacag gagcatgaat agaataaaag ttgcaatact
     3121 gtttgggggt tgctcagagg agcatgacgt atcggtaaaa tctgcaatag agatagccgc
     3181 taacattaat aaagaaaaat acgagccgtt atacattgga attacgaaat ctggtgtatg
     3241 gaaaatgtgc gaaaaacctt gcgcggaatg ggaaaacgac aattgctatt cagctgtact
     3301 ctcgccggat aaaaaaatgc acggattact tgttaaaaag aaccatgaat atgaaatcaa
     3361 ccatgttgat gtagcatttt cagctttgca tggcaagtca ggtgaagatg gatccataca
     3421 aggtctgttt gaattgtccg gtatcccttt tgtaggctgc gatattcaaa gctcagcaat
     3481 ttgtatggac aaatcgttga catacatcgt tgcgaaaaat gctgggatag ctactcccgc
     3541 cttttgggtt attaataaag atgataggcc ggtggcagct acgtttacct atcctgtttt
     3601 tgttaagccg gcgcgttcag gctcatcctt cggtgtgaaa aaagtcaata gcgcggacga
     3661 attggactac gcaattgaat cggcaagaca atatgacagc aaaatcttaa ttgagcaggc
     3721 tgtttcgggc tgtgaggtcg gttgtgcggt attgggaaac agtgccgcgt tagttgttgg
     3781 cgaggtggac caaatcaggc tgcagtacgg aatctttcgt attcatcagg aagtcgagcc
     3841 ggaaaaaggc tctgaaaacg cagttataac cgttcccgca gacctttcag cagaggagcg
     3901 aggacggata caggaaacgg caaaaaaaat atataaagcg ctcggctgta gaggtctagc
     3961 ccgtgtggat atgtttttac aagataacgg ccgcattgta ctgaacgaag tcaatactct
     4021 gcccggtttc acgtcataca gtcgttatcc ccgtatgatg gccgctgcag gtattgcact
     4081 tcccgaactg attgaccgct tgatcgtatt agcgttaaag gggtgataag catggaaata
     4141 ggatttactt ttttagatga aatagtacac ggtgttcgtt gggacgctaa atatgccact
     4201 tgggataatt tcaccggaaa accggttgac ggttatgaag taaatcgcat tgtagggaca
     4261 tacgagttgg ctgaatcgct tttgaaggca aaagaactgg ctgctaccca agggtacgga
     4321 ttgcttctat gggacggtta ccgtcctaag cgtgctgtaa actgttttat gcaatgggct
     4381 gcacagccgg aaaataacct gacaaaggaa agttattatc ccaatattga ccgaactgag
     4441 atgatttcaa aaggatacgt ggcttcaaaa tcaagccata gccgcggcag tgccattgat
     4501 cttacgcttt atcgattaga cacgggtgag cttgtaccaa tggggagccg atttgatttt
     4561 atggatgaac gctctcatca tgcggcaaat ggaatatcat gcaatgaagc gcaaaatcgc
     4621 agacgtttgc gctccatcat ggaaaacagt gggtttgaag catatagcct cgaatggtgg
     4681 cactatgtat taagagacga accatacccc aatagctatt ttgatttccc cgttaaataa
     4741 acttttaacc gttgcacgga caaacggttc tgttgcaaag ttttaaataa agaataaaat
     4801 ccct
//
LOCUS       DARBDV010000267         4355 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.guided.154, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000267 DARBDV010000000
VERSION     DARBDV010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.guided.154"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..1043)
                     /locus_tag="SCM56_003053"
     CDS             complement(<1..1043)
                     /locus_tag="SCM56_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEG1111754.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILD"
     gene            complement(1192..1371)
                     /locus_tag="SCM56_003054"
     CDS             complement(1192..1371)
                     /locus_tag="SCM56_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111755.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            1426..1509
                     /locus_tag="SCM56_003055"
     tRNA            1426..1509
                     /locus_tag="SCM56_003055"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1460..1462,aa:Leu,seq:gag)
     gene            complement(1749..3119)
                     /locus_tag="SCM56_003056"
     CDS             complement(1749..3119)
                     /locus_tag="SCM56_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111756.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELI
                     FSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLI
                     FLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLF
                     TELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLI
                     MGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFI
                     ITVINR"
     gene            complement(3833..4123)
                     /locus_tag="SCM56_003057"
     CDS             complement(3833..4123)
                     /locus_tag="SCM56_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1111757.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
BASE COUNT         1426 a          859 c          652 g         1418 t
ORIGIN      
        1 tcatccaaaa tattgaatcg ctttgtatca atttttggga atatataaaa agcagctttt
       61 ggttttacgg ctgaaagacc aggaatatca ttgattgcat tatagatata ttctctttgt
      121 tcatagatcc ttccgcctgg caggagcagc tcatcgacac tttgataacc gcctagcgcg
      181 gtttggataa tttgctgaga caatacattg gaacacaaac gcatggagga caacatattc
      241 agcccttcta tatagccttt gacgtgtgac ttatctccac ttaggaccat ccagcctact
      301 cgaaatcctg ctacacgatg agatttggat agaccgttca gcgtaacaac gaacaaatca
      361 ggcgctaatg tagcaatcgg aatatgttct agaccatcca tcactaagcg atcatagatt
      421 tcgtcggaat aaataatcag gttattttgt cgagctactt caacgatttc ttctaataat
      481 tcttttggat aaagtgcccc agtaggatta ttaggattaa tgataacaat agcttttgtc
      541 cgactcgtta tttttgattt gatatcatca atatcaggat accattctgc ctcttcatcg
      601 caaatataat gaacaggtgt gcctccagct aatgcaatag aagccgtcca caacggatag
      661 tccggcattg gaaccagtac ttcatctcca ttgtccagca acccctgcat acacatcgtg
      721 atcaattcgc tcactccgtt gccggtgtag atatcattga tcgtcacatt cggaaaatgt
      781 ttcagttggc aatattgctc aattgctttt ctagcagaaa agatcccctt ggaatctgag
      841 tagccttctg agtcacgggc attcatgatc aaatcacgaa tgacttcgtt tggtgcttca
      901 aatccaaaag gtgcgggatt tcccgtgttc aattttaaaa tactaatgcc ctcttcttgc
      961 atccgctccg cttcttctag aacgggaccg cgaacatcgt aactgacacc ttctaatttg
     1021 ttggattttt caaattctct catcacttag ccaatccctc atttcaaaaa gttagaactt
     1081 tccaaatttt ttgttattta ctatttcaat atcatactcc atcctagaaa attttgccac
     1141 ttatttagga aaaacgtaag tattaaaaaa acacaaacaa gcgttgagaa cttaattgtc
     1201 atgatagtca ttccatagat tcgctgccca atccattgca attccacaaa tgattcctag
     1261 cataaaacca agcaaaaaac gtccgaatgc aagtccaatc aatacaccta agatcattcc
     1321 cagtcctgaa aattctccgt catatttctc catcttaata agtcctccca tttttgagct
     1381 tgacaactat gtaaaacctc gctatcatta gtgtgttatt attacgcaga tgtgttggaa
     1441 ttggcagaca agcatgattg agggtcatgt gagcgttagc tcgtgtgggt tcgagtccca
     1501 tcatctgcac tacaactcgt gaagttaatt ttgcttcacg agtttttttg tttgaacgtt
     1561 tctttatgcc attatataca tatcactgta tcctttacaa aaagaacgta caatcatgta
     1621 aacagcctga ggtgagtata tgaaggtcgg aaacaagcta ctcgtagaaa cttgcaagtg
     1681 ttaaaacaat taatcgaaaa acgagtaaaa tccctcacct aatactaaag cgagggattt
     1741 tttgtatatt atctgtttat caccgttatt ataaaggtac aaataccaag cacggttaat
     1801 ccaaattgca taaattcatt tttttgtaca ctcatgcttt tcattagctc tttaactgcc
     1861 attagtacta agatggcgat aacaacataa agtatatagt acgtccgcat tccggaagcc
     1921 catactgtgg gagaaaatcc cataattaac cttgaacaaa atcccattat catcaaaata
     1981 actaaaaaaa tagaagtttt cttattgtca aaacttaaat aaattccaac aattaaacat
     2041 agtaataatg ccaagactac taaagtagct atccaagtac ctggataaaa aagtgacagt
     2101 ttcgttccta actctgtaaa gagattattc aggttactac tactccaaat cattgccctt
     2161 ttatttccat tgacgtaagt aaagctttga cccattgtat taccaaaata aataatcagg
     2221 tttaagaaaa acggtaatgc agtcaatata cgcacataat agttttgaca tttcctatat
     2281 gttagaagga aaattaagaa aaatagcaat aaaaataaga tattcatgtc taaaaaaaac
     2341 ggctttccaa atgatgaaat acctaaatct aacttattaa gaaagttgaa atttttatat
     2401 tcaggaaacc atttattaat ttcatgtgca gcacgtaatg cattccctgg agttgtcaaa
     2461 gagaaaatta attctgctaa gcttattatt gaaaatactg ataacttata attatatcgt
     2521 ccattgacaa tcaaatataa actaactata cttgttagaa caaaaaaaca ggcgttgact
     2581 tgttcttgat tagttaaaaa tatcaataaa ggaatactta cccaatatat ttttctattt
     2641 atttcttctc ctcttaagag ttgaaaaaac ggataaaaag ttagtaaact aaatgctact
     2701 ggccactgat agttcaaggt ggttgctctc caaccggttt cccccattac tgtcaatggt
     2761 attaaacagt atatagagca aaccaataat aaatttttaa catttatttt ctcaaaaaca
     2821 tacttgcaaa gtaatactac aattcctaac ataatcgtag aatttaataa tttccatagc
     2881 ataaagtgtt tagaaaagaa cattaagaat agttctataa taattcttga actccatgtc
     2941 ttataatgtt ctatcgaagc acccaaatag ccaagttcat ctaatctact tgcataaact
     3001 acgtcatccg ctctcatagt cggcatatac atattaaaga gtaaaaagat accccaaatc
     3061 cctattagta acaagatcag ttctttgtta ttcgttattt ttctattatg tttgttcatt
     3121 tttatactcc ttcttatatt tcctaataga gtataacata gctttaagca ttacttaaag
     3181 ataacttaaa agttatcttt taaatactta attgttaaat accttatttg atgcaaaaga
     3241 aatctacgat agtcaacaaa aaaattttca ttatatccag aaaaattttt cttagttaag
     3301 gacctatgga ttgcagtaat gcagaattcg tcgaataaac tgccaaagac atacaatcat
     3361 attgctttta aaattgacga acaagatata gattcgtttg tctcaaaaat ccaaatgtta
     3421 ggtttaactg tcgaaccagg acgttctcgt gtgaaaggcg aggcttcttc aatttacttt
     3481 tacgactatg ataatcacct cttcgagtta cataccggta ctctccagga acgtttaaaa
     3541 tcctacaatt ccactacctg agactagtaa tgactagtct ctttttatgg cctctgatcc
     3601 ccccattgag cgaatactaa tagggcttcg aattgatctg ttcaatcttt aagcaatatc
     3661 cctcaaatga gtttggaaag tttagtgatt catcacaaat acttaaatac atgtagttat
     3721 atatagattg aggttcttgt gagcgtatgc tcgtgtgggt tcaaatccca tcgtctgcat
     3781 ccatctttcc aaaacaagcg gctaggacat ttttgtctca gccgcttgtt ttctagttta
     3841 ttctttcatc ttgtcggacc ttttttcttg ggtatagact tttctcatat tctaccacaa
     3901 ttttgggttt ccctaacaca tgataggatc tcacaacaaa ttgctttcta ttattataat
     3961 aacctgtaac gactaacttc atcccttcgt ctacttcagc taggaagttc aaactatggg
     4021 aagcaatcaa acaattgacc ccatttaaac tgaaacggat caacggtctg gcactaactt
     4081 gcaaagtacg gatcttatct actgttccat attttttctc cataaccttc acctcaatac
     4141 cattatacga acatacattc cctttgtaaa gaagaaactc tgttttatct ttttgacttt
     4201 ttgtctatta cctttcttaa aatcatattg gcacttcctg cgaagatgaa ggacatgtac
     4261 taaaaaaaag aggcagcagt atagattact gttgcttctt tactaattta ggtttacttg
     4321 ttaagaaaga ttatttacgc aaatgttctt caaca
//
LOCUS       DARBDV010000268         2872 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.guided.175, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000268 DARBDV010000000
VERSION     DARBDV010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.guided.175"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            159..1637
                     /gene="msr(C)"
                     /locus_tag="SCM56_003058"
     CDS             159..1637
                     /gene="msr(C)"
                     /locus_tag="SCM56_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HEG1111758.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            1684..2130
                     /locus_tag="SCM56_003059"
     CDS             1684..2130
                     /locus_tag="SCM56_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HEG1111759.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2329..2844)
                     /locus_tag="SCM56_003060"
     CDS             complement(2329..2844)
                     /locus_tag="SCM56_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEG1111760.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
BASE COUNT          902 a          492 c          601 g          877 t
ORIGIN      
        1 gtagaatagg tgaaagctat gacagatttg gtgatttagt catagttatt tcgttcagag
       61 taggaaaaga tgtgatgcag tttttttgca aacagatttt tctgctcttt tttagtatcg
      121 ataattgttg ttttacatga aataggagtg taaaacatat ggaaaattta gcagtaaata
      181 taacaaatct acaagtcagc tttggcaacc agctagaact atctattgat tctcttcgtg
      241 tctatcagca agatcgtata gggatcatag gagaaaatgg tgtcggtaaa tcgactttgc
      301 tcaaactaat agccggtgaa ctttttcccg atcatgggaa aatccaaaca gagatcacct
      361 tcaactacct gcctcaatta acctatctcg ctgaagcaaa ggacctaaat ttggaattag
      421 ccagtcattt ccagttaaga ctggaagaaa cttcagagcg gaaatggagc ggaggggaag
      481 aacgaaagat cgagttgata cgtcttcttt cttcttatga acaagggatg cttctagacg
      541 agccaacaac ccatctagat agaaaaagta ttgatcgact gattgaagag cttcgttatt
      601 attatggtac gctggttttt gttagtcatg atcgctattt tctagatgag ctagcatcga
      661 aaatctggga agtaaaagac ggagaaatcc gagagttttc ggggaattat agtgcctatc
      721 tcactcaaaa ggaattggag aaaaagactc agctacgaga agcagagtcg atcatgaaag
      781 agaaaaaacg attggaaaaa tcgatccaag aaaagaaaaa acaagcggaa aagttggaaa
      841 aagtgtccag taaaaagaaa aagcaacaaa tcagactgga tcggttgtct tcctctaaac
      901 aaaaagacag tgtacaaaaa gccatccaaa agaatgcgaa aacattagag agaagactcc
      961 aaaaaatagg agaaacaacc aagccgcagc agatgaaaca aatccgtttt ccagtaccaa
     1021 aatctcttga actccacagc cgctatccca tcatgggaca aaatgtccaa ttggaaagaa
     1081 gtgggagaac attactggta aatggtgatt ttcagttttc tttaggtaaa aaaatcgcga
     1141 ttgtcggtga aaatggttca ggtaagacaa ccttattaga acatatccgc aaacaaggag
     1201 aaggaatcct tctctctccg aaagtaagct ttcaagtata tcagcaaaag ggttatcaaa
     1261 tgacatctga agaatccatc attcgttttg tcatgagaca aacagagttt tcagaatcgc
     1321 ttgtccgtag tttgctgaat cacttagggt ttgctcagga aactttgacg aaaccgttat
     1381 gtacattaag tgggggagaa gcgacccgtc tgacgattgc tttgcttttt actaagccaa
     1441 gtaatgtgtt gctgttagat gaaccgacta attttattga tatggcaacg atcgaagctt
     1501 tagagaagct aatgcaaata tatccgggaa cgattttgtt tacttctcat gattcttact
     1561 ttgtcgagcg tacggctgat gaagtttatg aaataaaagg gcagaaaata aaaaaagtac
     1621 ttacgagaaa tttttaaatg tgctacgatg attttcgtga caaatttaaa ggagtggtac
     1681 agattgccta caaataaaaa agaaggaata atttttacaa caatgatgtg ttttttgatg
     1741 gtcttgggga tgagcgcgta taatttatgg ctgcatcaag atcttcattt atcaagtttg
     1801 ttgattggtc tagtgcctgg attcgtggtg gctttcattt tggatgtctt catcatcggt
     1861 gtgctggcga aaaaaattgc ctttaaacta ccagtaaatc cagaaagcaa ggtccaaatg
     1921 atcttaacta tttcatgctt gatggtttta ggaatggtga catgtatgtc tttattcggt
     1981 gtgatcatgg aacaaggaat acctgatcaa ttattctctg cttatatgaa tgcttggaaa
     2041 atgaatgtga ttgccgcttt accgttgcag ttgatcatcg ttggcccgct ttcaagaagt
     2101 gtgctaaaag caattcaaag taatgaatga tggaacactg ttatttgaaa aaaggccttg
     2161 gcatgctttt tgtcatagtc tcttgaatgc agttctagaa aaatagtgat ccgaacgata
     2221 ggttttggcc tgtcgttcgg atcgcttttt cttttccaac tgtattgctc ttttattgaa
     2281 gataatttgc tcttcaatag atatttctag aaaggttctg tttatccctt actcaaaaaa
     2341 ctgctgcatt tgttctctta cttgctgaca ccattggagt tcctgctgac aaagcataat
     2401 tctgcgctgg atcacaacat aacgtccaaa ttcttgtttc ttgtctagcg agaggtcatt
     2461 caattttttt tgattttttt gcatgatttt gttcagctgt tgttctctag tgaatagcag
     2521 tccagtagct gttgttttat cgagcgtcga aaaagcatag atttttgcta gaaattcatc
     2581 tttttgcgta ggagcgggag tttcctcttt gatccattcc tctacaagag cacgtccttc
     2641 atcagtcaag tggtagatct ttttttgaga gtgctcgtct ttgtccaaaa cggtcacata
     2701 accctgctgg tctaacttgg caagcgttgt atagatctga ctgtgatggg cttcccaaaa
     2761 aattgtgatc agctgcttca attcataccc agaacgtggt ttggataaaa gcacgcataa
     2821 taatatatag gctaaagtat tcataaaaat cctacctctc gtcttatttt ct
//
LOCUS       DARBDV010000269         2742 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.guided.177, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000269 DARBDV010000000
VERSION     DARBDV010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.guided.177"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..179
                     /locus_tag="SCM56_003061"
     CDS             <1..179
                     /locus_tag="SCM56_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1111761.1"
                     /translation="SLRERQITANVVSKPKKSSRSEEILIKTLKRRVMELEKENKKLQ
                     NQIQKLYGDLYNKE"
     gene            333..1115
                     /locus_tag="SCM56_003062"
     CDS             333..1115
                     /locus_tag="SCM56_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HEG1111762.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(1241..1972)
                     /gene="erm(A)"
                     /locus_tag="SCM56_003063"
     CDS             complement(1241..1972)
                     /gene="erm(A)"
                     /locus_tag="SCM56_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HEG1111763.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(2030..2089)
                     /locus_tag="SCM56_003064"
     CDS             complement(2030..2089)
                     /locus_tag="SCM56_003064"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HEG1111764.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            2481..>2742
                     /locus_tag="SCM56_003065"
     CDS             2481..>2742
                     /locus_tag="SCM56_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEG1111765.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNM"
BASE COUNT          926 a          402 c          498 g          916 t
ORIGIN      
        1 aatcccttcg tgagcgtcaa ataacagcaa atgtagtctc aaaacccaag aaaagttctc
       61 gttcggagga aatccttatt aaaaccttaa aaagaagagt aatggaatta gaaaaagaaa
      121 ataaaaaatt acagaaccaa attcaaaaat tatatggaga tctgtataat aaagaataat
      181 tattaatctg tagacaaatt gtgaaaggat gtacttaaac gctaacggtc agctttattg
      241 aacagtcatt taggtatatg tccaatctag ggtaagtaaa ttgagtatca atataaactt
      301 tatatgaaca taatcagtta cgaggtgaaa tcatgagcaa tttgattaac ggaaaaatac
      361 caaatcaagc gattcaaaca ttaaaaatcg taaaagattt atttggaagt tcaatagttg
      421 gagtatatct atttggttca gcagtaaatg gtggtttacg cattaacagc gatgtagatg
      481 ttctagtcgt cgtgaatcat agtttacctc aattaactcg aaaaaaacta acagaaagac
      541 taatgactat atcaggaaag attggaaata cggattctgt tagaccactt gaagttacgg
      601 ttataaatag gagtgaagtt gtcccttggc aatatcctcc aaaaagagaa tttatatacg
      661 gtgagtggct caggggtgaa tttgagaatg gacaaattca ggaaccaagc tatgatcctg
      721 atttggctat tgttttagca caagcaagaa agaatagtat ttctctattt ggtcctgatt
      781 cttcaagtat acttgtctcc gtacctttga cagatattcg aagagcaatt aaggattctt
      841 tgccagaact aattgagggg ataaaaggtg atgagcgtaa tgtaatttta accctagctc
      901 gaatgtggca aacagtgact actggtgaaa ttacctcgaa agatgtcgct gcagaatggg
      961 ctatacctct tttacctaaa gagcatgtaa ctttactgga tatagctaga aaaggctatc
     1021 ggggagagtg tgatgataag tgggaaggac tatattcaaa ggtgaaagca ctcgttaagt
     1081 atatgaaaaa ttctatagaa acttctctca attaggctaa ttttattgca ataacaggtg
     1141 cttactttta aaactactga tttattgata aatattgaac aatttttggg aagaataaag
     1201 cgtcctcttg tgaaattaga gaacgcttta ttactttaat ttagtgaaac aatttgtaac
     1261 tattgaaaat agaaagaaat tgttccttcg atagtttatt aatattagtg acatttgcat
     1321 gcttcaaagc ctgtcggaat tggtttttag tgaaaagaac acgatattca cggtttaccc
     1381 acttataaac aaaagatcga tactttttgt agtccttctt tgaaatcaat ggttgatgtc
     1441 gttcaagaac aatcaataca gagtctacac ttggcttagg atgaaaatat agtggtggta
     1501 cttttttgag catttttata tccatctcca ccattaatag taaacccaaa gctcgttgca
     1561 gattttgcaa tcttttcgca aatcccttct caacgataag atagctatat ttagcctgac
     1621 tttcaaaggt aattcttttg acaatatccg tactgatgtt ataaggaata ttaccatata
     1681 tcttatagtt tatatgtttt gggaaggaaa attttagaat atccgtttga atcactttta
     1741 tattctcaga ggggtttacc gcttctttag tcacttgaca taagcctcca tcaatttcta
     1801 tagcagtaac tgatcgactc attttgacta gctctttggt aaaatgtcct tttcctgatc
     1861 cgatttctat tacgttgtct tgtttactga tattcgtgtg attcaatatt tcttttacat
     1921 gctttttaga agtaataaaa ttttgcgtgt ctttagggtt tttctggttc attataacct
     1981 tctccttact ggttataatg aactgacatt aagcgttcat tataaccaat taattttgat
     2041 ttggttgata acgaacttta ttaataacaa atatagaaaa agtacccata cttttatctc
     2101 ctttccattt ttttcattat gaatgagata aagtaatttc tactactgcg atactggtgc
     2161 acataattga aacctcctta tgtaactgaa tatgattgta tcatcttttc attttagaat
     2221 aaacctttat atgtaatttt gcacttgaca cttcagatgg acgttttctt caaaataata
     2281 tgttttgaaa cttgataaga gaagccactg tctttactaa aaagtaaagg ctgcgataaa
     2341 ggacagtgac tttaataaag ttgcactgtt gtattgtctt tggataaaat tgtctaaagg
     2401 gaagttttat ccccaacgaa aaaacatcta aagtggtaag taatgaaaga atgtcgataa
     2461 agtaggaatg ggggatcttt ttgaatacaa tagaatcaca gtttgataag gttgcagaag
     2521 attacgattt cgtgaatgag cttttgaatg attattcatt ctttgtgtct aatatgtctc
     2581 caaagaaagg cagagcatta gatatcggat gtggctcggg tttgttagtg gagaaattag
     2641 caagttatta tgatgaagtg gtagggattg atatttctaa tcaaatgctc gatcttgcca
     2701 aatctaaacg tcaactaaca aatacggtct atctgaatat ga
//
LOCUS       DARBDV010000270         1953 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.guided.194, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000270 DARBDV010000000
VERSION     DARBDV010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.guided.194"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            111..194
                     /locus_tag="SCM56_003066"
     CDS             111..194
                     /locus_tag="SCM56_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HEG1111766.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            319..1056
                     /gene="erm(B)"
                     /locus_tag="SCM56_003067"
     CDS             319..1056
                     /gene="erm(B)"
                     /locus_tag="SCM56_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEG1111767.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1319..>1953)
                     /locus_tag="SCM56_003068"
     CDS             complement(1319..>1953)
                     /locus_tag="SCM56_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEG1111768.1"
                     /translation="ELVKLYEKDVVKHVTPYSNRMTEALISRLSDQGYNLVIEGTGRT
                     TDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYETMYADDPMTARATPKQAHD
                     IVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGK
                     EIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTPPIPKTPKLPGI"
BASE COUNT          635 a          323 c          330 g          665 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaaccaa aattttgtat aataggaatt
       61 gaagttaaat tagatgctaa aaatttgtaa ttaagaagga gggattcgtc atgttggtat
      121 tccaaatgcg taatgtagat aaaacatcta ccgttttgaa acagactaaa aacagtgatt
      181 acgcagataa ataaatacgt tagattaatt cctaccagtg actaatctta tgacttttta
      241 aacagataac taaaattaca aacaaatcgt ttaacttctg tatttattta tagatgtaat
      301 cacttcagga gtgattacat gaacaaaaat ataaaatatt ctcaaaactt tttaacgagt
      361 gaaaaagtac tcaaccaaat aataaaacaa ttgaatttaa aagaaaccga taccgtttac
      421 gaaattggaa caggtaaagg gcatttaacg acgaaactgg ctaaaataag taaacaggta
      481 acgtctattg aattagacag tcatctattc aacttatcgt cagaaaaatt aaaactgaac
      541 actcgtgtca ctttaattca ccaagatatt ctacagtttc aattccctaa caaacagagg
      601 tataaaattg ttgggaatat tccttaccat ttaagcacac aaattattaa aaaagtggtt
      661 tttgaaagcc atgcgtctga catctatctg attgttgaag aaggattcta caagcgtacc
      721 ttggatattc accgaacact agggttgctc ttgcacactc aagtctcgat tcagcaattg
      781 cttaagctgc cagcggaatg ctttcatcct aaaccaaaag taaacagtgt cttaataaaa
      841 cttacccgcc ataccacaga tgttccagat aaatattgga agctatatac gtactttgtt
      901 tcaaaatggg tcaatcgaga atatcgtcaa ctgtttacta aaaatcagtt tcatcaagca
      961 atgaaacacg ccaaagtaaa caatttaagt accgttactt atgagcaagt attgtctatt
     1021 tttaatagtt atctattatt taacgggagg aaataattct atgagtcgct tttgtaaatt
     1081 tggaaagtta cacgttacta aagggaatgt agataaatta ttaggtatag ggcacctcta
     1141 ataactacca attaattcac ctcaaaatga aattagaaaa aaacagcgaa atttttaaat
     1201 ctatttctta tcgatacaaa ttcctcgtag gcgctcggga ccccaacatg aggaaatgtt
     1261 tttataaaaa aagagtagtt accgtttttt ggtaactact cttttccttt tttaaggttt
     1321 aaatacctgg aagtttaggt gttttgggta ttggtggtgt aggtagctgc aagctttcca
     1381 ttttttgttg aattgcttta aattcagggg tttcttggtg ttgattttga accatttttt
     1441 gctctattct ctccaaagtc ggttgaattt ctttccctga tactttacga tttaattctc
     1501 tttctaaggt ctctttcgga ctaatggaag gcgtttctaa gcttgaatag agtttcactc
     1561 cttctctatt gtaaagcctt atatcgctaa ataagcccgt tttatgaagg gtttctaaat
     1621 tggtcggtaa gtttttgaca acaatatcat gcgcttgttt tggtgttgcc ctagctgtca
     1681 ttggatcatc tgcatacatg gtttcatatc gttcaatcgt tcctaagtat gattcaattt
     1741 tcggtactgc catgacgtat atttttgttt cataaccttt agattgaagc attgtggctg
     1801 tttgaatggg aacgtctgtt gttcgtcctg ttccttcaat cactaaatta tagccttgat
     1861 cgcttaaacg gctaataagt gcttctgtca tgcgattcga ataaggggta acgtgtttta
     1921 ctacgtcttt ttcatagagt ttcactaatt cgt
//
LOCUS       DARBDV010000271         1421 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.guided.208, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000271 DARBDV010000000
VERSION     DARBDV010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.guided.208"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1350
                     /locus_tag="SCM56_003069"
     CDS             <1..1350
                     /locus_tag="SCM56_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HEG1111769.1"
                     /translation="RDFILYLKLCNRTESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQ
                     IYKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANA
                     VILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMK
                     YLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLN
                     TNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFL
                     RQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVF
                     EGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFG
                     EDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          597 a          114 c          239 g          471 t
ORIGIN      
        1 agggatttta ttctttattt aaaactttgc aacagaaccg aatcattaaa aaaacattat
       61 acagagcctt gggaagatga agtttttaga gtaattattg aatataacaa tgttcctatt
      121 ggatatggac aaatatataa aatgtatgat gagttatata ctgattatca ttatccaaaa
      181 actgatgaga tagtctatgg tatggatcaa tttataggag agccaaatta ttggagtaaa
      241 ggaattggta caagatatat taaattgatt tttgaatttt tgaaaaaaga aagaaatgct
      301 aatgcagtta ttttagaccc tcataaaaat aatccaagag caataagggc ataccaaaaa
      361 tctggtttta gaattattga agatttgcca gaacatgaat tacacgaggg caaaaaagaa
      421 gattgttatt taatggaata tagatatgat gataatgcca caaatgttaa ggcaatgaaa
      481 tatttaattg agcattactt tgataatttc aaagtagata gtattgaaat aatcggtagt
      541 ggttatgata gtgtggcata tttagttaat aatgaataca tttttaaaac aaaatttagt
      601 actaataaga aaaaaggtta tgcaaaagaa aaagcaatat ataatttttt aaatacaaat
      661 ttagaaacta atgtaaaaat tcctaatatt gaatattcgt atattagtga tgaattatct
      721 atactaggtt ataaagaaat taaaggaact tttttaacac cagaaattta ttctactatg
      781 tcagaagaag aacaaaattt gttaaaacga gatattgcca gttttttaag acaaatgcac
      841 ggtttagatt atacagatat tagtgaatgt actattgata ataaacaaaa tgtattagaa
      901 gagtatatat tgttgcgtga aactatttat aatgatttaa ctgatataga aaaagattat
      961 atagaaagtt ttatggaaag actaaatgca acaacagttt ttgagggtaa aaagtgttta
     1021 tgccataatg attttagttg taatcatcta ttgttagatg gcaataatag attaactgga
     1081 ataattgatt ttggagattc tggaattata gatgaatatt gtgattttat atacttactt
     1141 gaagatagtg aagaagaaat aggaacaaat tttggagaag atatattaag aatgtatgga
     1201 aatatagata ttgagaaagc aaaagaatat caagatatag ttgaagaata ttatcctatt
     1261 gaaactattg tttatggaat taaaaatatt aaacaggaat ttatcgaaaa tggtagaaaa
     1321 gaaatttata aaaggactta taaagattga ttatataata tatgaaaagc tattataaaa
     1381 gacggttctg ttgcaaagtt ttaaatctac tatcaaataa g
//
LOCUS       DARBDV010000272         1421 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4432
            SAMEA114569962-rid20112443.guided.209, whole genome shotgun
            sequence.
ACCESSION   DARBDV010000272 DARBDV010000000
VERSION     DARBDV010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569962
            Sequence Read Archive: ERR12203787
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972646.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972646.1
            Genome Coverage       :: 159x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:52:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 27 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 6 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569962-rid20112443.guided.209"
                     /isolate="VRE4432"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1350
                     /locus_tag="SCM56_003070"
                     /note="aph(2'')-Ia"
     CDS             <1..1350
                     /locus_tag="SCM56_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HEG1111770.1"
                     /translation="RDFILYLKLCNRTESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQ
                     IYKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANA
                     VILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMK
                     YLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLN
                     TNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFL
                     RQMHGLDYTDISECTIDNKQNVLEEFILLRETIYNDLTDIEKDYIESFMERLNATTVF
                     EGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFG
                     EDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          596 a          114 c          239 g          472 t
ORIGIN      
        1 agggatttta ttctttattt aaaactttgc aacagaaccg aatcattaaa aaaacattat
       61 acagagcctt gggaagatga agtttttaga gtaattattg aatataacaa tgttcctatt
      121 ggatatggac aaatatataa aatgtatgat gagttatata ctgattatca ttatccaaaa
      181 actgatgaga tagtctatgg tatggatcaa tttataggag agccaaatta ttggagtaaa
      241 ggaattggta caagatatat taaattgatt tttgaatttt tgaaaaaaga aagaaatgct
      301 aatgcagtta ttttagaccc tcataaaaat aatccaagag caataagggc ataccaaaaa
      361 tctggtttta gaattattga agatttgcca gaacatgaat tacacgaggg caaaaaagaa
      421 gattgttatt taatggaata tagatatgat gataatgcca caaatgttaa ggcaatgaaa
      481 tatttaattg agcattactt tgataatttc aaagtagata gtattgaaat aatcggtagt
      541 ggttatgata gtgtggcata tttagttaat aatgaataca tttttaaaac aaaatttagt
      601 actaataaga aaaaaggtta tgcaaaagaa aaagcaatat ataatttttt aaatacaaat
      661 ttagaaacta atgtaaaaat tcctaatatt gaatattcgt atattagtga tgaattatct
      721 atactaggtt ataaagaaat taaaggaact tttttaacac cagaaattta ttctactatg
      781 tcagaagaag aacaaaattt gttaaaacga gatattgcca gttttttaag acaaatgcac
      841 ggtttagatt atacagatat tagtgaatgt actattgata ataaacaaaa tgtattagaa
      901 gagtttatat tgttgcgtga aactatttat aatgatttaa ctgatataga aaaagattat
      961 atagaaagtt ttatggaaag actaaatgca acaacagttt ttgagggtaa aaagtgttta
     1021 tgccataatg attttagttg taatcatcta ttgttagatg gcaataatag attaactgga
     1081 ataattgatt ttggagattc tggaattata gatgaatatt gtgattttat atacttactt
     1141 gaagatagtg aagaagaaat aggaacaaat tttggagaag atatattaag aatgtatgga
     1201 aatatagata ttgagaaagc aaaagaatat caagatatag ttgaagaata ttatcctatt
     1261 gaaactattg tttatggaat taaaaatatt aaacaggaat ttatcgaaaa tggtagaaaa
     1321 gaaatttata aaaggactta taaagattga ttatataata tatgaaaagc tattataaaa
     1381 gacggttctg ttgcaaagtt ttaaatctac tatcaaataa g
//
